LOCUS       DAXIHS010000001        62195 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXIHS010000001 DAXIHS010000000
VERSION     DAXIHS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 62195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.001"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            81..293
                     /locus_tag="ACFOF2_000001"
     CDS             81..293
                     /locus_tag="ACFOF2_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106406.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(572..1204)
                     /gene="mtrR"
                     /locus_tag="ACFOF2_000002"
     CDS             complement(572..1204)
                     /gene="mtrR"
                     /locus_tag="ACFOF2_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC7106407.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            1608..2846
                     /gene="mtrC"
                     /locus_tag="ACFOF2_000003"
     CDS             1608..2846
                     /gene="mtrC"
                     /locus_tag="ACFOF2_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC7106408.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVDE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQADVQTASEAKTASEAE"
     gene            2858..6061
                     /gene="mtrD"
                     /locus_tag="ACFOF2_000004"
     CDS             2858..6061
                     /gene="mtrD"
                     /locus_tag="ACFOF2_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC7106409.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIASKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            6116..7519
                     /gene="mtrE"
                     /locus_tag="ACFOF2_000005"
     CDS             6116..7519
                     /gene="mtrE"
                     /locus_tag="ACFOF2_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC7106410.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREATYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(7711..7908)
                     /locus_tag="ACFOF2_000006"
                     /pseudo
     CDS             complement(7711..7908)
                     /locus_tag="ACFOF2_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(7908..8114)
                     /locus_tag="ACFOF2_000007"
                     /pseudo
     CDS             complement(7908..8114)
                     /locus_tag="ACFOF2_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            8721..9500
                     /locus_tag="ACFOF2_000008"
     CDS             8721..9500
                     /locus_tag="ACFOF2_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC7106411.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(9686..9943)
                     /locus_tag="ACFOF2_000009"
     CDS             complement(9686..9943)
                     /locus_tag="ACFOF2_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC7106412.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
     gene            complement(9987..10361)
                     /locus_tag="ACFOF2_000010"
     CDS             complement(9987..10361)
                     /locus_tag="ACFOF2_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106413.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            complement(10503..11837)
                     /gene="gdhA"
                     /locus_tag="ACFOF2_000011"
     CDS             complement(10503..11837)
                     /gene="gdhA"
                     /locus_tag="ACFOF2_000011"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC7106414.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            12221..13015
                     /locus_tag="ACFOF2_000012"
     CDS             12221..13015
                     /locus_tag="ACFOF2_000012"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC7106415.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIYELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            13024..13621
                     /locus_tag="ACFOF2_000013"
                     /pseudo
     CDS             13024..13621
                     /locus_tag="ACFOF2_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(13856..15193)
                     /locus_tag="ACFOF2_000014"
     CDS             complement(13856..15193)
                     /locus_tag="ACFOF2_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC7106416.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
     gene            complement(15580..16644)
                     /locus_tag="ACFOF2_000015"
     CDS             complement(15580..16644)
                     /locus_tag="ACFOF2_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC7106417.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(16645..17403)
                     /locus_tag="ACFOF2_000016"
     CDS             complement(16645..17403)
                     /locus_tag="ACFOF2_000016"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC7106418.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(17478..17553)
                     /locus_tag="ACFOF2_000017"
     tRNA            complement(17478..17553)
                     /locus_tag="ACFOF2_000017"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17518..17520),aa:Ile,seq:cat)
     gene            complement(17607..18716)
                     /locus_tag="ACFOF2_000018"
     CDS             complement(17607..18716)
                     /locus_tag="ACFOF2_000018"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC7106419.1"
                     /translation="MEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFSSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPDLP
                     AINLTGSGSRWETEKHIAASSSFAFGGSNCVLIIG"
     gene            complement(18720..19284)
                     /locus_tag="ACFOF2_000019"
                     /pseudo
     CDS             complement(18720..19284)
                     /locus_tag="ACFOF2_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            19331..19831
                     /locus_tag="ACFOF2_000020"
                     /pseudo
     CDS             19331..19831
                     /locus_tag="ACFOF2_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            19834..19917
                     /locus_tag="ACFOF2_000021"
                     /pseudo
     CDS             19834..19917
                     /locus_tag="ACFOF2_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            20198..20632
                     /locus_tag="ACFOF2_000022"
     CDS             20198..20632
                     /locus_tag="ACFOF2_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106420.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            20639..21154
                     /locus_tag="ACFOF2_000023"
     CDS             20639..21154
                     /locus_tag="ACFOF2_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC7106421.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPSYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            21174..22652
                     /gene="ubiD"
                     /locus_tag="ACFOF2_000024"
     CDS             21174..22652
                     /gene="ubiD"
                     /locus_tag="ACFOF2_000024"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC7106422.1"
                     /translation="MKYKDLRDFIVMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTSYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVDNTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(22717..24831)
                     /locus_tag="ACFOF2_000025"
     CDS             complement(22717..24831)
                     /locus_tag="ACFOF2_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC7106423.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRILTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            24922..25815
                     /locus_tag="ACFOF2_000026"
     CDS             24922..25815
                     /locus_tag="ACFOF2_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC7106424.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            25955..26809
                     /gene="folP"
                     /locus_tag="ACFOF2_000027"
     CDS             25955..26809
                     /gene="folP"
                     /locus_tag="ACFOF2_000027"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC7106425.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAEQLLKEGADILDIGGESMRPGAADYVSPEEEWARVAPVLAEVAGWGVPISLDTR
                     RTVIMEKALALGVVDIINDVAALTDKGAVELLARQADTGICLMHMQGLPENMQINPKY
                     QDVVGEVVRYLKERAAECIAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     FPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKA
                     WEALGINL"
     gene            26939..28273
                     /gene="glmM"
                     /locus_tag="ACFOF2_000028"
     CDS             26939..28273
                     /gene="glmM"
                     /locus_tag="ACFOF2_000028"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC7106426.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEANYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMENALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            28462..29145
                     /locus_tag="ACFOF2_000029"
     CDS             28462..29145
                     /locus_tag="ACFOF2_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC7106427.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            29212..30204
                     /locus_tag="ACFOF2_000030"
     CDS             29212..30204
                     /locus_tag="ACFOF2_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC7106428.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWSYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TASGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGAEDVHKELVRLKLV"
     gene            30212..30982
                     /locus_tag="ACFOF2_000031"
     CDS             30212..30982
                     /locus_tag="ACFOF2_000031"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC7106429.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDFVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            31086..32162
                     /gene="prfA"
                     /locus_tag="ACFOF2_000032"
     CDS             31086..32162
                     /gene="prfA"
                     /locus_tag="ACFOF2_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC7106430.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            32287..33285
                     /locus_tag="ACFOF2_000033"
     CDS             32287..33285
                     /locus_tag="ACFOF2_000033"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC7106431.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQIGDCGNAVRADG"
     gene            complement(33363..34658)
                     /gene="serS"
                     /locus_tag="ACFOF2_000034"
     CDS             complement(33363..34658)
                     /gene="serS"
                     /locus_tag="ACFOF2_000034"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC7106432.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            34824..35348
                     /locus_tag="ACFOF2_000035"
     CDS             34824..35348
                     /locus_tag="ACFOF2_000035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC7106433.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            35415..37400
                     /gene="parE"
                     /locus_tag="ACFOF2_000036"
     CDS             35415..37400
                     /gene="parE"
                     /locus_tag="ACFOF2_000036"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC7106434.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(37474..37878)
                     /locus_tag="ACFOF2_000037"
     CDS             complement(37474..37878)
                     /locus_tag="ACFOF2_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC7106435.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(37970..39070)
                     /gene="aroC"
                     /locus_tag="ACFOF2_000038"
     CDS             complement(37970..39070)
                     /gene="aroC"
                     /locus_tag="ACFOF2_000038"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC7106436.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFGSVVQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            39213..40301
                     /gene="trmA"
                     /locus_tag="ACFOF2_000039"
     CDS             39213..40301
                     /gene="trmA"
                     /locus_tag="ACFOF2_000039"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC7106437.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKKLGDAWQQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEQTLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTPHIESSVLLKKKIL"
     gene            40322..41515
                     /locus_tag="ACFOF2_000040"
     CDS             40322..41515
                     /locus_tag="ACFOF2_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC7106438.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEAELVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFAYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(41580..41655)
                     /locus_tag="ACFOF2_000041"
     tRNA            complement(41580..41655)
                     /locus_tag="ACFOF2_000041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41620..41622),aa:Arg,seq:ccg)
     gene            41940..42779
                     /locus_tag="ACFOF2_000042"
     CDS             41940..42779
                     /locus_tag="ACFOF2_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC7106439.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            complement(43245..46097)
                     /gene="gcvP"
                     /locus_tag="ACFOF2_000043"
     CDS             complement(43245..46097)
                     /gene="gcvP"
                     /locus_tag="ACFOF2_000043"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC7106440.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIAKRIHALASAFADALVSDGIKVVHKVFFDTVTVDFGSK
                     EKTDQVFAAALESGYNLRRVNDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQNDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAKVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASADQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(46128..46487)
                     /locus_tag="ACFOF2_000044"
     CDS             complement(46128..46487)
                     /locus_tag="ACFOF2_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106441.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLVGGFPLSLPLAVLLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            46486..46710
                     /locus_tag="ACFOF2_000045"
     CDS             46486..46710
                     /locus_tag="ACFOF2_000045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC7106442.1"
                     /translation="MPSEPAGRLFQKMPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            complement(46762..47403)
                     /locus_tag="ACFOF2_000046"
     CDS             complement(46762..47403)
                     /locus_tag="ACFOF2_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC7106443.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(47393..47944)
                     /locus_tag="ACFOF2_000047"
     CDS             complement(47393..47944)
                     /locus_tag="ACFOF2_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC7106444.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(47948..48466)
                     /locus_tag="ACFOF2_000048"
     CDS             complement(47948..48466)
                     /locus_tag="ACFOF2_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC7106445.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(48466..50127)
                     /gene="pqiB"
                     /locus_tag="ACFOF2_000049"
     CDS             complement(48466..50127)
                     /gene="pqiB"
                     /locus_tag="ACFOF2_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC7106446.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     DLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(50120..51580)
                     /locus_tag="ACFOF2_000050"
     CDS             complement(50120..51580)
                     /locus_tag="ACFOF2_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC7106447.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDSGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGMAFNETEKHD"
     gene            51918..52547
                     /locus_tag="ACFOF2_000051"
     CDS             51918..52547
                     /locus_tag="ACFOF2_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC7106448.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKKLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            52741..53349
                     /locus_tag="ACFOF2_000052"
     CDS             52741..53349
                     /locus_tag="ACFOF2_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC7106449.1"
                     /translation="MSNSSFKLSALAIACGLALSACSSGGGDAPSVKPTPTVPGASNP
                     APANPEPSAPAASPLKTGELGEKYFYGEGEITKLVINGETINLTVADEQLSRRVKLWT
                     DFDKIITSQGFENLIYGAAKLADGSYALFVQGKLSDSLPGDTVTYSGDVLHFRSRNLT
                     EGNWFNEGNSFRAAGSFTATVNFAGKSLNGKINSGDGLYMGT"
     gene            53365..53550
                     /locus_tag="ACFOF2_000053"
                     /pseudo
     CDS             53365..53550
                     /locus_tag="ACFOF2_000053"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(53880..54356)
                     /locus_tag="ACFOF2_000054"
     CDS             complement(53880..54356)
                     /locus_tag="ACFOF2_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC7106450.1"
                     /translation="MLAACQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(54490..55845)
                     /gene="yegQ"
                     /locus_tag="ACFOF2_000055"
     CDS             complement(54490..55845)
                     /gene="yegQ"
                     /locus_tag="ACFOF2_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC7106451.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRSIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDAEGWATVEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            56170..56958
                     /locus_tag="ACFOF2_000056"
     CDS             56170..56958
                     /locus_tag="ACFOF2_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC7106452.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVGALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(57004..57603)
                     /locus_tag="ACFOF2_000057"
     CDS             complement(57004..57603)
                     /locus_tag="ACFOF2_000057"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC7106453.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            57731..58348
                     /gene="gmk"
                     /locus_tag="ACFOF2_000058"
     CDS             57731..58348
                     /gene="gmk"
                     /locus_tag="ACFOF2_000058"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC7106454.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            58407..58613
                     /gene="rpoZ"
                     /locus_tag="ACFOF2_000059"
     CDS             58407..58613
                     /gene="rpoZ"
                     /locus_tag="ACFOF2_000059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC7106455.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            58706..60862
                     /locus_tag="ACFOF2_000060"
     CDS             58706..60862
                     /locus_tag="ACFOF2_000060"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC7106456.1"
                     /translation="MPVPQPTAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGENLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGKHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(60929..62113)
                     /gene="coaBC"
                     /locus_tag="ACFOF2_000061"
     CDS             complement(60929..62113)
                     /gene="coaBC"
                     /locus_tag="ACFOF2_000061"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC7106457.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
BASE COUNT        14525 a        15573 c        17176 g        14921 t
ORIGIN      
        1 aagaatgccg gagaagaatg cagtttacat tttgaatgat attgttatta attgtaaata
       61 aatattggaa ggtaaatgat atgaaaaatg aaattcaaaa aatcatggac aaatataatc
      121 catggcatga agatgatttt gagtcgtatg aagacattgc caaagatgta tcgctgatga
      181 cagataaaac gttcattgaa cattatttgt tagaagttta ttcagaagaa aacggacatt
      241 ttgaccaaga gaatatccat gcgatgatag gggaaattaa aaatgcaatt tagtgaatat
      301 gagacctttg caaaaatagt ctgttaacga aatttgacgc ataaaaatgc gccaaaaaat
      361 tttcgattgc ctaaaacctt cctaatattg agcaaaaagt aggaaaaatc agaaaagttt
      421 tgcattttga aaatgagatt gagcataaaa ttttagtaac ctatgttatt gcaaaggtct
      481 caatatgtat ttttagaaga aggctttgtg tcattttgat gccgtctgaa agggtttgtt
      541 cgtttcagac ggcattgcta ccaaggcttg attatttccg gcgcaggtcg ggatggtttt
      601 ccaagttgtc catcattatc ccgatgatgc gcggggcggt tttgcccaaa tcgaaacgtt
      661 cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt tgatttgatg aagataactg
      721 ccgtttcctt gtccaaatcg tcagccaaat cctgattttc caccgcttcg gtcaaaacgg
      781 cggtaatttt ctcgcgccag attgcctgat gcttgcgggc aatggcgata acggcggcgt
      841 tttgctccgt gtgttcgcat tttaaaaaca ggatgttgtg gaatttgtag tagatgtcgt
      901 tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg gaataccgcc caagaccctc
      961 cttcggcatc ttcggcatct tgcgcgatgc agttttcgat gtcgtcgcag atacgttgga
     1021 acagcgcgtc gaacaagtct tccttatttt tgaaatgcca atagagcgcg ccgcgcgtta
     1081 cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc aatccctttg cggtaaaagg
     1141 tttccaaggc ggcaagcatc aggtgttctt tggttttcaa ggcttcggtt ttggtttttc
     1201 tcataatggc gttttcgttt cgggtcggtt tgatgagggc ggattataaa aaagactttg
     1261 taaccatgca atcgtgtatg tataatgaaa cccatgaaat tgagactaca tctcaacttt
     1321 gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt cgggtatcaa agcaggcggt
     1381 ttttatatcc tgaaatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     1441 cagtacaaat agtacggaac cgattcactt agtgcttcag caccttagag aatcgttctc
     1501 tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata agaatgcctg
     1561 ttgattggca aaacccttta catcaaacca gacagatagg ataaataatg gctttttatg
     1621 cttttaaggc gatgcgtgcg gccgcgttgg ctgccgccgt tgcattggta ctgtcgtctt
     1681 gcggtaaagg cggagacgcg gcgcagggcg ggcagcctgc tggtcgggaa gcccctgcgc
     1741 ccgtcgtcgg tgtcgtaacc gtccatccgc aaaccgtcgc attgaccgtc gagttgccgg
     1801 ggcgtttgga atcgctgcgt accgccgatg tccgtgcgca agttggcggc attatccaaa
     1861 aacgcctgtt ccaagaaggc agttatgtcc gtgccggaca gccgctgtat cagatcgaca
     1921 gttccactta tgaagcaaat ctggaaagcg cgcgcgcgca actggcaacg gctcaggcaa
     1981 cgcttgccaa agcggatgcg gatttggcgc gatacaagcc tttggttgcc gccgaagccg
     2041 tcagccggca ggaatacgat gctgcggtaa cggcgaaacg ttctgccgag gcaggtgtca
     2101 aagcagcaca ggcggcgatc aaatctgccg gcatcaatct gaaccgttcg cgcattaccg
     2161 cgccgatttc cggctttatc ggtcagtcca aagtttccga aggtacgctg ttgaatgcgg
     2221 gcgatacgac cgtgctggca accatccgcc aaaccaatcc gatgtatgtg aacgttaccc
     2281 agtctgcatc cgaagtgatg aaattgcgcc gtcagatagc cgaaggcaaa ctgctggcgg
     2341 cggatggtgt gattgcggtc ggcatcaaat ttgacgacgg cacagtttac cctgaaaaag
     2401 gccgcctgct gtttgccgat ccggccgttg acgaatcgac cggtcagatt accctgcgcg
     2461 ccgccgtacc gaacgatcag aacatcctga tgcccggcct gtatgtgcgc gtgctgatgg
     2521 accaagtggc ggtggataac gcatttgttg tgccgcagca ggcggtaacg cgcggtgcga
     2581 aagataccgt gatgattgtg aatgcccaag gcggtatgga accccgcgag gtaacggtcg
     2641 cgcaacagca gggtacgaat tggattgtta cgtcgggtct gaaggacggg gacaaggtgg
     2701 ttgtggaagg catcagtatc gccggtataa cgggtgcgaa aaaggtaacg cccaaagaat
     2761 gggcgccgtc tgaaaaccaa gccgccgcgc ctcaagctga cgttcagacg gcatctgaag
     2821 ccaaaactgc ttctgaagcg gaataaggaa ggcatcgatg gctaaatttt ttatcgaccg
     2881 ccctattttt gcgtgggtta tttcgatttt cattattgcg gcgggtattt tcggcatcaa
     2941 aagcctgcct gtttcgcaat atccgtccgt cgccgccccg accattactt tgcacgccac
     3001 ttatccgggt gcgtccgcgc aggtaatgga agacagcgtg ctttccgtga tcgagcggaa
     3061 tatgaacggc gtggaaggtt tggattatat gtccacttcc gccgattcga gcggcagcgg
     3121 cagcgtgagc ctgaccttta cgcccgatac cgacgagaat ctggcgcagg tggaagtgca
     3181 gaacaagctt tccgaagtat tgagcacgct gccggcaacc gtccagcaat acggcgtaac
     3241 cgtatccaag gcgcgttcca atttcctgat gattgtgatg ctttcgtcgg atgtgcagtc
     3301 aaccgaagag atgaacgact acgcgcagcg taatatcgtt cccgaactgc agcgtatcga
     3361 aggcgtgggg caggtacgcc tgttcggcgc gcaacgcgcg atgcggattt gggttgatcc
     3421 taagaaactg caaaactaca atttgtcgtt tgccgatatc ggcagcgcgc tgtccgccca
     3481 gaacgtccag atttcagcgg gttctatcgg ttcgcttccc gccgttcgcg gacagacggt
     3541 tacggctacc gtaacggcgc aagggcagtt gggtacggca gaagaattcg gcaacgtcat
     3601 cctccgcgcc aataccgacg gttctaatgt ttacctgaag gatgtggcaa gggtcggact
     3661 gggtatggaa gactattctt cctcaacccg tctgaacggt gtaaatacca ccggtatggc
     3721 ggtgatgctg tccaacagcg gcaatgcgat ggcgacggca aaggcggtta aagaacgcat
     3781 ggcgacgttg gaaaaatact ttcctcaggg tatgagctgg aaaacccctt acgatacttc
     3841 caaattcgtc gaaatttcga ttgaaaaagt gattcatact ttaatcgaag cgatggtgct
     3901 ggtgtttgtc gtaatgtatc tcttcctgca aaacatccgt tatacgctga ttccgaccat
     3961 cgtcgtaccg atttcgctgt tgggcggttt cgctttcatc tcttatatgg gcatgtcgat
     4021 taacgtactg accatgtttg cgatggtttt ggtcatcggc atcgtggtcg atgacgcgat
     4081 tgtggtggtt gaaaacgtcg agcgcattat ggcgggtgaa ggcttgccgc ccaaagaagc
     4141 gaccaaaaaa gcgatgggtc agatttcggg cgcggtcatc ggtattaccg ccgttctgat
     4201 ttccgtgttc gtaccgttgg cgatgttcag cggggcgacg ggcaatattt acaaacagtt
     4261 tgccctgacg atggcgtcat caatcgcatt ctccgccttc cttgcgctga cccttacccc
     4321 tgctttgtgt gccacaatgt tgaagacaat cccgaaaggg catcacgaag agaaaaaagg
     4381 tttcttcggc tggtttaaca agaaattcaa cagttggacg cacggttacg aaggccgggt
     4441 tgccaaagtg ctgcgtaaga ctttccgcat gatggttgtc tatatcggct tggcggttgt
     4501 gggcgtgttc ctgtttatgc gcctgccgac ttcgttcctg ccgaccgaag accaaggctt
     4561 cgtcatggtc agcgtgcaac tgcctgcagg agcgacccaa gagcgcacca atgcgacttt
     4621 ggcgcaagtt acccaactgg cgaaaagcat tcctgaaata gaaaacatca ttaccgtttc
     4681 cggcttcagc ttttcgggca gcggtcagaa tatggcgatg ggttttgcca tattgaaaga
     4741 ttggaacgag cgtaccgcgc ccggcagcga tgccgttgcg attgccagca agctgacggg
     4801 tatgatgatg gggacgctta aagacggttt tggcatcgcc gtcgtcccgc ctccgattct
     4861 ggagttgggc aacggttcgg gtctgagcat caacctgcaa gaccgcaaca ataccggcca
     4921 taccgcattg ctggcgaagc gcaacgagtt gattcagaaa atgcgtgcca gcggtttgtt
     4981 tgaccccagc accgtccgtg ccggcggttt ggaagactcg ccgcagttga aaatcgacat
     5041 caaccgtgcc gccgcggcgg cgcaaggcat ttcgtttgcc gacatccgca ccgcattggc
     5101 aagtgcgctg agttcgtctt atgtcagcga cttcccgaac caaggccgtc tgcaacgcgt
     5161 gatggtgcag gccgacgagg atgcccgtat gcagcctgcc gatattttga acctgaccgt
     5221 gccgaacaaa tccggcgtcg ccgtaccgct ttccaccatt gctactgttt cttgggaaaa
     5281 cggtacggaa cagagcgtac gcttcaacgg ttatccttcg atgaagctgt ccgcttcgcc
     5341 tgcaaccggc gtttccaccg ggcaggctat ggcggcggtt caaaaaatgg ttgacgaatt
     5401 gggcggcggt tacagcctgg agtggggcgg acagtcgcgc gaagaggcaa aaggcggctc
     5461 gcaaaccctg attttgtacg gtttggcggt tgcagccgta tttttggtac ttgccgcgct
     5521 ttatgaaagc tggtcgattc cgctggcggt catccttgtg attccgttgg gtttgatcgg
     5581 tgcggctgcg ggcgtaaccg ggcgcaacct gtttgaagga ctgttgggca gcgttccctc
     5641 gttcgccaac gacatctact ttcaagtcgg tttcgttacc gtgatgggtt tgagtgcgaa
     5701 aaacgcgatt ttgattatcg agtttgccaa agaccttcaa gcgcaaggga aaagcgcggt
     5761 tgaagccgct ttggaagcag cccgcctgcg tttccgtccg attatcatga cctcgttcgc
     5821 ctttattttg ggcgtggttc ccctgtatat tgccggtggt gcaagttctg ccagccagcg
     5881 cgccatcggc acgaccgtat tctgggggat gctcatcggc acgctcttgt ccgtgttcct
     5941 tgttccgctt ttctatgtgg tggtgcgcaa attcttcaaa gaaaccgcgc acgaacacga
     6001 aatggcagta aaacacgccg ccgaagcggg catcaccggt tcggacgaca gccaacatta
     6061 agcaaccatg ccgtctgaac gcccacgggt tttcagacgg catcaggact tttttatgga
     6121 tactacattg aaaaccacct tgacttctgt tgcagcagcc ttcgcattat ccgcctgcac
     6181 catgattccc caatacgagc agcccaaagt cgaagttgcc gaaacgttca aaaacgatac
     6241 cgccgacagc ggcatccgtg cggtcgattt aggttggcat gactattttg ccgacccgcg
     6301 cctgcaaaag ctgatcgaca tcgcactcga gcgcaatacc agtttgcgta ccgccgtatt
     6361 gaacagcgaa atctaccgca aacaatacat gattgagcgc aacaacctcc tgcccacgct
     6421 tgccgccaat gcgaacgact cgcgccaagg cagcttgagc ggcggcaatg taagcagcag
     6481 ctacaaagtc ggactgggtg cggcatctta cgaactcgac ctgttcggac gcgtccgcag
     6541 cagcagcgag gcagcactgc aaggctattt tgccagcacc gccaaccgcg atgcggcaca
     6601 tttgagcctg attgccaccg ttgccaaagc ctatttcaac gaacgttatg ccgaagaagc
     6661 gatgtctttg gcgcagcgtg tcttgaaaac gcgcgaagca acctacaagc tgtctgaatt
     6721 gcgttacaag gcaggcgtga tttccgccgt cgccttacgc cagcaggaag ccctcatcga
     6781 atccgccaaa gccgattatg cccatgccgc gcgcagccgc gaacaggcgc gcaatgcctt
     6841 ggcaaccttg attaaccaac cgatacccga cgacctgccc gccggtttgc cgttggacaa
     6901 gcagtttttt gttgaaaaac tgccggccgg tttgagttcc gaagtattgc tcgaccgtcc
     6961 cgatatccgt gctgccgaac acgcgctcaa acaggcaaac gccaatatcg gtgcggcacg
     7021 cgccgccttt ttcccatcca tccgcctgac cggaagcgtt ggtacgggtt ctgccgaatt
     7081 gggcgggctg ttcaaaagcg gcacgggcgt ttggtcgttt gcgccttcca ttaccctgcc
     7141 gatttttacc tggggtacga acaaggcgaa cctcgatgtg gcaaaactgc gccaacaggc
     7201 acaaatcgtt gcctatgaat ccgccgtcca atccgcattt caagacgtgg caaacgcatt
     7261 gaccgcgcgc gagcagttgg ataaagccta tgatgcttta agcaaacaaa gccgcgcctc
     7321 taaagaagcg ttgcgtttgg tcggtctgcg ttacaaacac ggcgtatccg gcgcgcttga
     7381 tttgctcgat gcggaacgca gcagctattc ggcggaaggt gcggctttgt cggcacaact
     7441 gacccgcgcc gaaaaccttg ccgatttgta caaggcactc ggcggcggat tgaaacggga
     7501 tacccaaacc gacaaataac cggtcgggca ataaaatgcc gtctgaaacg ataaggtttc
     7561 agacggcatt tctttcttta tagtggatta acaaaaacca gtacagcgtt ggctcgcctt
     7621 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gttgtactat ttgtactgtc
     7681 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatgtttga cggttgcggt
     7741 caggctgcgc ggcgtgccgt aaacgtgaat gtcgggcata gggcggtagc gtttgttgaa
     7801 cacgttttca aagtccaaac cgatgcgggt ggatttgccg attttgtaat gcgcggtcaa
     7861 atccagcgtg gcgtaggggc gttgggtcag tgcggctgcg gcggcaggga agaaaccgcc
     7921 atacccgccg gcatcccgaa gccgaatgcg gcaacggcaa gcgcaatcag cagcgtataa
     7981 agccccgcct gatagccttt cagcttcagg acggtcagca cggcaaataa aaagacgatg
     8041 ggtaagagtg cggcggcggc agtcagatac aggctgccgc cgattgccgt gtagttttga
     8101 acccaagttt ccataattga acatctccga aaatattttt ctaatcgtcg gcaatagtgg
     8161 tcaaaccaat taaagcaacg ttgcattact ttgcgaaact ttaatattca ggtcaataga
     8221 gtggattaaa tttaaaccag tacggcgttg cctcgcctta ccgtactatc tgtactgtct
     8281 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tatatttttg ggcggttcgg
     8341 cagatttgaa tcggagcttt cgtttaaatc ctgtcaaaac aaatatttgc ataaacaaaa
     8401 attgtagttt ggtgtagttt tttcctgtgt ttcgggggcg gtgcgaggta aatgccgttg
     8461 acggcgggaa cggcgtgtca ttagaatccg ccctgattgg tcagtccaat ttgatgtttg
     8521 atgtatagtg gatgaacaaa aaccagtacg gtgttgcctc gccttagctc aaagagaacg
     8581 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg
     8641 cttcgtcacc ttgtcctgat ttttgttaat ccactatatt tcggaagggt tctgcaggga
     8701 atctttataa aggaggagta atgaaactgg taaggcctca gaaaatcagc gatcaggtat
     8761 tgtcggtatt ggaggagcgc attgcggaag gggtttacgc ggaagggggc aagattccgc
     8821 ccgaacgcgt tttggcggaa gagttcggcg tgtcccgccc gtcggtcagg tcggcattga
     8881 atgttttggt tgcccgtcag gttttggaag cgaggcaggg agacggttat tatgtttcgg
     8941 tcaagccaca gcaggatttt ttgcaaagtt ggcaggagct tttgggcaag cattccaatt
     9001 gggaacagga tgtttttgat ttcagctgcc acatcgaggg ctgtatggcg gcattggcgg
     9061 cagaacgccg gacggatgcc gatttgaagc ggattgggtt ttggcttgaa aagtttgagg
     9121 aggcgtgcga aagcggcaat ctggaacatc agagcgaagc cgatgtcagc ttccatcaga
     9181 cgattgcgga tgcggcgcac aatttgttgt tcagccattt gtcgggcggt ttgttgaaaa
     9241 tgctgtaccg gcagacgcgc agcagcctta tttatctgaa tcaggaagaa gatccgcgtc
     9301 cgaaactgat ggcgcagcac cgcgtgctgt atgaggcgat atcgaaccgt cggccgggcg
     9361 aggcttcgga ggcggcaaag gcacatttga attatgtggc aagcagcatc atgaaagaca
     9421 gggaatatca gagccgtaac cgccacgcgg atactttggc gcaaaacgat ttgaagcgcg
     9481 tgcaggattg ggaggtatga ataccgttta gacggtctgg catattcgga tgtatcggta
     9541 tagtgaacta aatttaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc
     9601 tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggctcg
     9661 ccgccttgtc ctgatttttg ttaattcact gctgaccaat cattatccgc cagataaagt
     9721 attctgcttc atccttgtct atttccagca ggagaaagcc aaccacttgc ttatcccaat
     9781 agattgccct cggaaacaca tcttcatttt cccggtagag ccatgcgtca gctaaagagc
     9841 gtacatttgg agccacaaaa cgtgctcgtt catcagcttc agatattttc aaatccagca
     9901 ctgcctgaaa actgctctca tctactaatt tcagctcaat cattttttct ccttttgaat
     9961 aaatagggtt ttgaggtgat taaagactaa tctaactcat ataaaaggta gttatcaaaa
    10021 tttatgattc ctttgaggta atctcggtct gctttagaca actcaaattc tggaaaagtc
    10081 gcaattaaac tggacaaata aaaataattc gggtgcctac gataagaaaa acctgcttga
    10141 ccgaatttcc cttgacaaaa atcataggca agtttcaatt cttgacttgt gacaccgtct
    10201 aaaattttat ctaactctga aagctgactt ttatcagtat tttctatcat agctttcact
    10261 gtatttacta aaacaaaaag aaaatcatta gtattatcca gtaggattgg tctgttagat
    10321 aacttattct ttaactccat atagtattgg tccataatca tttcttcctc tccactccca
    10381 aactatccaa caattctgga tacaaatagt atcacccgat tcgaacgggc ggataatgaa
    10441 atgccgtctg aacagcattt gcggttcgga cggcattttg tttgcggagc ggcggcgttt
    10501 gcttagaagc cttgcgccag catcgcatcg gcaactttga cgaaaccggc aatgttcgca
    10561 ccattgacgt agtttactgt gtcgccgact ttgccgtatt tcagacagga ttcgtggatg
    10621 ctttgcatga tgccgaacag gcgttggtcg acttcttcac gagtccaaga caggcggatg
    10681 gcgttttggc tcatttccaa acctgaagtt gctacgccgc cggcattgga ggcttttccc
    10741 ggggcgtaga ggatgccggc tttgataaat tgctcgaccg cgcccaaagt cgacggcata
    10801 ttcgcacctt cggcaacgac gtagcagccg tttgccaaca gggttttggc ggcttcttcg
    10861 tccaattcgt tctgggtcgc gcagggcagg gcgatttcgg cggcgacgcc ccacggtttt
    10921 tggttttcaa agtattgcag accttgctct ttggcgtagg tggcaacgcg ttcgcggcgg
    10981 acttctttca attcgatcaa ggcggcgagt tgcgcttcgg tcataccgct gtcggggaag
    11041 aggacgaagc cgttggagtc ggaaacggtc agcactttcg cgcccagttg gatggctttt
    11101 tcggcggcgt attgcgccac gttgccggaa ccggaaatca ggacgcgttt gccttcaaaa
    11161 ctatcgttgc gggtttgcag catcgcttgg gcgaaataga cgcagccgta gccggtcgct
    11221 tcgggacgga tgaggctgcc gccccattcc aaacctttgc cggtcaggac ggaagaaaac
    11281 tcgttgcgga tttttttgta ttgtccgaac agatagccga tttcgcgtcc gcctacgccg
    11341 atgtcgccgg ccggaacgtc ggtgtccgcg ccgatgtggc ggtagagttc ggtcataaag
    11401 gcttggcaga agcgcattac ttcggcatcg gatttgcctt tggggtcgaa gtcggaaccg
    11461 cctttgccgc cgcccatagg cagggtggtc aaggcgtttt tgaacacttg ttcaaaagcg
    11521 aggaatttca atacgcccaa atcgacggtc ggatggaagc gcaggccgcc tttgtaagga
    11581 ccgatggcgg aactcatttg cacgcggtag ccccggttga cttggacttg ccctttatcg
    11641 tcctgccagg ttacgcggaa catgacgacg cgttcgggtt cgacgatgcg ttccagcagg
    11701 ctttgctggg tgtatttcgg attttttgcc aaaaacggat cgagactcat gaagacttct
    11761 tcaaccgcct gatggaacgg ctcctgattg ggattgcgtt gtttgaggtt ggcaaacagg
    11821 gtgttcaggt cagtcattcg gatactcctt ttggttgata gtgtactaat gggaaaacaa
    11881 atttgtaggt gggaatataa acctattttt ttctgatgcc aagcccgatt tggcaaaaat
    11941 tgttgacatt attttttcca tgttttcgat gtttgatgtt tgttgccgat aattaatcaa
    12001 taatcataaa ttaatttcgt aaataaaaat attattgaaa ttttattcta agtaattaaa
    12061 ctagattttt tgattccaat caaggtaatt ttgttttccc accgggtttt actgtggaaa
    12121 aggactgaaa ttgccaacaa tcctgcctgt ctgtatccgt gttttgggca ttatccggaa
    12181 atgttacaat atgcgccgtt taatttgatg aaagactgct atgaaagcct atttggactt
    12241 gatgcgccac gtcctcgaca acggtacgga caaatcagac cgcaccggta cgggcacgcg
    12301 ctccgtattc ggttatcaga tgcgttttga cttgggcaaa ggttttccgc tgctgacgac
    12361 caaaaagctg catctgcgtt cgattatcta cgagctgctt tggtttctca aaggcgatac
    12421 caatatcaaa tacctaaagg acaacaatgt ttccatttgg gacgagtggg cggacgaaaa
    12481 cggcgactta ggcccggttt acggctacca gtggcgtaac tggcccgcgc ccgacggcag
    12541 gcatatcgac caaatcgcca atgtggtgga acaaattaag aaaaaccccg actcgcgccg
    12601 cctgattgta tcggcgtgga atccggcttt ggtcgacgaa atggccttgc cgccctgcca
    12661 cgcgctgttt cagttttacg ttgccgacgg caaactgtcc tgccagcttt accagcgtag
    12721 tgcagatatt ttccttggtg tgccgtttaa tattgccagc tacgcattgt tgaccatgat
    12781 gatggcgcaa gtgtgcggat tggaggcggg cgagtttgtc catacgtttg gcgacgcgca
    12841 tttgtaccgc aaccatttcg agcaggccgc attgcagttg gaacgcgaac cgcgcgcctt
    12901 gccagtgatg aaaatcaatc ctgaagtcaa agatttgttt tccttcaagt ttgaagattt
    12961 cgagttggaa ggttacgacc cccatccgca catcaaggcg gcagtgtcgg tttaggaggc
    13021 ggtatgggca ggcatttcgg gcgcaggcgt tttttgacag ttgccgccgt tgcggcggcg
    13081 ggcgcggcgg tttctttcct gccgaatcct tttgccgccg atgatgaaaa acgcaataaa
    13141 gatgaaaaac gcaatgaaaa tgtgttcttc tggaaaggtg tcgcgctggg ttccggtgcg
    13201 gagctgcgcc tgttcggtgt ggacgacaga caggcggcgg atttggtcaa caaggttttg
    13261 gcggaagtgg cgcgtttgga aaaaattgtt cagcctttac cgtgaagaca gcctgatcag
    13321 ccgcctgaac agggacggtt atctgacttc gccgtcggcg gattttttgg aactgttgag
    13381 cctgtgccgc gatattcacg cgctgacgga cggcgcgttc gatccgacag tgcaggcgtt
    13441 gtggaaactg tatgccgatt attttgccgc ccgtcccgat gcggaaacgc cgcctcctca
    13501 aaacagcgtg aaggaagccc tgaaacgggt cggcttcggc aaagtctcgt ttgacgaccg
    13561 tgaaatccgt tttgccgaaa agggtatggg tttgtccttg aacggcatcg cgcaaggcta
    13621 tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga gaacgattct
    13681 ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg
    13741 tcgccttgtc ctgatttttg ttaatccact atactttgaa cgaaataggc tgaacctgaa
    13801 gcaaaaatgc cgtctgaaag gctttcagac ggcatttttg ctgtttggat ggcttttaaa
    13861 ggatgtccaa agaattgatg aaaacaataa taatggcaat cggggcgagg tagcgcaagg
    13921 tattcagcca cagcagcaac actgcccgtg gtacggtgct gcccgcgccg gcatctttta
    13981 acacagactg cctgtcctga atccaagcgg taaatacggc aacgctcaat gcgccgacag
    14041 gcatgatgac tgcagaaata aggtagtccc acaaatcaaa aatggttttg ccgaaaacct
    14101 taaactcacc ccatacgccg aaagacagcg cggacgggat gccgacaata aaaatagccg
    14161 tgccgataag ccaagtatgt tttttgcgtt tgcgctcgtc ttggcggatg gttgcggcga
    14221 tgaccgtttc caacatcgaa aatgccgaag tcagcgtggc gaaaacgacc agcagcataa
    14281 ataccgcaaa caaaaccgta ccgaacggca ttttcataaa caccgccggc aatacgataa
    14341 aaatcaatcc cggcccctgg ctcggttcaa aaccgaaggc gaacaccgcc ggaaaaatca
    14401 ccaaacctgc caacagcgaa accaagaggt tcatccacat aatcgtatgc cccgagcgga
    14461 acatatcctg atcttttccc aaatacgaag cataggtaat catagcggaa acgccgatgc
    14521 tcaaggcaaa aaacgcctga cctaaagccg taatcatcgt atcggcttta aagtacgacc
    14581 aattcggttt gagcaggaaa gacacgccct ccattgcacc cggcagcgtc agcgaacgga
    14641 ttgccagcgc aataaagagg ataaacagcc ccggcatcag ataacggttt gccttttcaa
    14701 tgccgtctga aatgccgcct ttgaccaccc aaaccgtaat cagcataaac agtgcctgat
    14761 aggacagcga acctgccgga ttggaaatcg tcgcgccgaa caaggcttca aagtccgcgc
    14821 cggtatgaac cgcccccgtg aaactgtgga cgacataatt taatacccat ccgccgacca
    14881 cgctgtaaaa cgacagcaaa ataaagcagg cggaaacgcc catgcgcccg acccaaagcc
    14941 attgcgtact cggacgcaga gccctgaagg aatcgacggc atttttaccg cctgtgcgcc
    15001 cgatataaaa ctcggcaagc tggacgggca gggcgaccaa gacggtaaat atcaaaaaca
    15061 gcaggaaaaa caccgcgccg ccgttggtgc ctgccgtata aggaaatttc caaatcgcgc
    15121 ccaaaccgat ggccgaacct gccgcagcaa gcacgaaacc gattttggac gaccaagaag
    15181 tatggttact cataaaattt attaagaaat gtgaagatag tatagtggat taacaaaaat
    15241 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt cacttggtgc
    15301 ttgagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggtttt
    15361 tgttagtcta ctatacattg taacaaagca cttgatgcgg cagcacaagc cggggaagac
    15421 gctgcaaaac tttgccaata ggctgcgtaa tatcggggga tattgggatt ttcttatttt
    15481 taaggatgga ttcggggtgt ttctttccaa tgaacgaata taatttggcg gtttgttgca
    15541 tcaaaggctt tcagacggca tatgccgtct gaaagcagtt tatattgcat ccaataattt
    15601 gtcggcggta tggttgatgg taaaacggtt ggcgacgcat tgatgtcctg ccgttgccag
    15661 tgcgtttttc gtttcagggt tcaagacgag gcgttcgatt tcgtccgcca aagattcagg
    15721 tgtcagcctg tccagcagaa tccccgattg atgatgttcg ataatttcct tttgcgcccc
    15781 caaagtattg gaaatcaccg ccgttcggca gtacatcgcc tcgcataatg acaaaccgaa
    15841 tgcctccggg acgaggctgg gcaaaaccac gacatcgctt tggcggtaaa acgaagcaag
    15901 tttttcggta aacccttcaa aagaaacaaa tggttctgct cctgaagcag atacgtcccg
    15961 cttcaggcgg cacatataat ccggatgtcc gtcccctgcc aatttgagcc tgatttgagg
    16021 atatttccga tgcagtatca cacaggcttc aatcaggttt tccaatcctt tttctggact
    16081 gatcctgccg gcgtaggcga cggtaaaaaa acggctgtcg ggtttttctt gagagggagg
    16141 gaaacggccg gtatcgatac cgttatgaac aatccggtat tttgctttaa agggattgtc
    16201 ggcggtttgc acatcgtaaa ccagacggga aacgcaaata aagcggtctg tgtttttctg
    16261 tatcaggcgg tggtaaaaat cggttttgtt ggcgacgaca ttatgtttga caaatatcag
    16321 gcgcaccccg gtcagttttt tcaaaagtat ggataaggcg gcaattttgc cggtgtgtat
    16381 catcaggtgg gaaatgcggt ttttccggat aaagcgggta agcgcgcaga gcgaaaacag
    16441 cccgttgagc gtgtgaaggc gcgttgtgaa aacggaagaa acttcggaaa aacgtctgtg
    16501 catcaactca ttatttttat tgacggcggt aaacatcgtg caaccccgaa gccccaatgc
    16561 ttttgaaaca tcatagacat actgttcgcc cccgccccaa atcttggccg atacgatatt
    16621 taatatcctg aattcttttt ccatttaaaa ttttccgcca gatttgtaca gataatatag
    16681 tttgacatat ttaatcatcg tgtaatagct gtggttgacg gacataatcc aacccatttt
    16741 tccatcgaga aaccctttgt tcaggatata aattttgaaa aaaccccaaa tcgggcggag
    16801 gataatgtcc ctaacgaaac gcacgggctt tccctgctct cggtatttct cggctgagat
    16861 ggaagtgtat ttgttgaatt tgttgaaata ttgttcccag ttgtcgtatg tgtaatggta
    16921 cataaaatgc ttcagacggc gtttggggta gggggtttgt acggtttcgt gtactttgcc
    16981 ttgcaccgaa ctgtcttttt tcggcatcag acggcatacg ctgtcgggac gcatcgcgcc
    17041 gtgtgtggcg ggatggttgg ggaaaaggtt gcggcgttcg acaaaatagg cggcataatc
    17101 gccggtttgg acaatttttg agatttcatc agatagttcc ggcgtgcagc gttcgtctgc
    17161 atcaatcagg aaaacccatt ctccgcctgc ctgttcgatg gcaaatgttt tttgcgctcc
    17221 gaaatccccg ttcaaatgcc gtctgaagac ttttgcaccc aaaccctcgg caatttcggc
    17281 agtattgtcg gtgctgtggt cgtcaataac gataacttct ttatcgaaac ggcaactttc
    17341 aatacattcc cgaatgtggt ttgcttcgtt tttggcaaca atcaatacgc tgactttttt
    17401 cattatcaag gtttctcaaa gcattttggt ttcacagggc ggcaaaaaag cccaaacata
    17461 cgtccgggct tgggaattgg tgggtccggt ggggttcgaa cccacgacca agggattatg
    17521 agtcccctgc tctaacccct gagctacaga cccgtcaaga gtaggcgcgt attgtatagc
    17581 atagcggtgg gattgacaaa cttatttcat ccgatgatta agacgcagtt gcttcctccg
    17641 aaggcaaacg acgagctggc ggcaatgtgt ttttcggttt cccagcggct gccgctgccg
    17701 gtcaggttga tggcgggcag gtcggggtcg ttctgcccgt cccaaagccg gggcggaagt
    17761 ttcccttcgg gattgctttg ccggtcggca atgccccacg cgaacgcggc ttcgattgcg
    17821 cccgccgcgc ccagcgtgtg tccggtttgc ggcttggtgg acgtgcaggg cgtattgttg
    17881 ccgaaaaccg ctgcaacggc acggctttcc atactgtcgt tgtggtgcgt cccggtgccg
    17941 tgcagattaa tccagccgat gtcttcgggc gcaagacctg cgtgttgcaa tgccgtctga
    18001 aaggcaagga ttgcgccttg cgcgtcgggg cggggcgtgg aaatatggta ggcatcgctg
    18061 ttggctccgt agccgacgag ttcggcgtaa attatcgcac cgcgccgttt ggcgtgttcc
    18121 aattcttcca gcacgaaaat ccccgcgcct tcgccgatga ccagcccgtc gcggttcgcg
    18181 tcgtatgggc gcggggtttt ttccggttcg ccgttgcggc ggctggcggc ataaagcgag
    18241 tcgaaaacat acacttcgga cgagaaaaat tcttcgcctc cgcccgccag catcatatcg
    18301 gtcagaccgt atttgatggc ttcgtaggca taacctatgc cttggctgcc ggacgaacac
    18361 gcgctcgatg tcgggatgat gcgtcctttc agcccgaaaa agatgccgat attggcggcg
    18421 gtggtgtgcg gcatcatacg cacataggtg ttggcactga agttgcgcga cgtgccggtc
    18481 agcaacaatt cgcccacatc gccgatgtct ttggtgctgc cgctggaaga gccgcaggca
    18541 acgcccatcc gtccgtcggt aatgctttcg tccccgagca aaccggcatc cgtcaaagcc
    18601 tgctccgccg catcgacgca caaatacgac acacgcccca tacttctgag ctgcttgcgc
    18661 gtccaatgtt tcggcggcgc gtaatcctca atcggcgcac ccagttgcgc ttccaattcg
    18721 gggaaacgtt cgtgccaatc cactatattt tcgcaagacg cgttttgcct gattccaaaa
    18781 gttctcaatg ccgttaatgt ggttttgacg gtctgcaaat tccttggaat ggttgatgcg
    18841 gtaatggata aaactgctca cgtccaactt gtcgcagctg ctcagactat cggtataaac
    18901 aatactgtcc ggcatgattt tctttttgat gacagggagt aacgtttcag acttggcatt
    18961 gtttacgaca acggtataga cccgtccgtt gcgtttcaga atgccgaaaa caaccacttt
    19021 tcctgccgca ccgcgaccac gtctgccttt acgccgtccg ccgaaatcgc tttcgtccgg
    19081 ctcgacagga tcctcaaaaa cctcatcggc agccaagacc aaatgatggt tgataaccgt
    19141 gcggatttta cggtagaaca gtgctgccga attgggatgg atacccaaaa tatcggcggc
    19201 agaacgggcg gtaacttcca gtacaaaaaa atggagcagt tctttctgta cttttttctt
    19261 taatttgcag tgtgttatct tcatatttcg agggtaacat atctgctaat ctagtaaaat
    19321 ttaatccact atagtttatg cctcgcacga cggggaaatg gcgcgcagtt tcgatttgtg
    19381 gttggaactg ttgaaatcgc aaatcgtgtc gccgacttcg ttcggtttgt cggtgcacaa
    19441 tgcaacggcg gggtaatggt cgatattgag gcgggatatg agcgagcaga cggcattggc
    19501 cgtgtgtgcc gacggtgtgg aaacggcatt ggcggaagcg gcatcactgt tggaagaggg
    19561 atgcgggtcg gttttggttt tggcggcgga cgatccgctg ccggaaggat atgccgtatc
    19621 ggcaacgcgc gcgccgtttg cttatgcttt ggcgatggtt ctgaccaaag ggacgcgtta
    19681 cagcctgact ttatctgctt cggacgatat gccgtctgaa gccggtatgc tgcccgaggc
    19741 atattggagc ggtttggaat gggtgcgctt tctgctgaac ggcagtcggg aatgccgccg
    19801 ggtatatcgc aatcgtgaat ggctgtggca gcccgcaagc tgcaggctga agatttccgc
    19861 aatgtccgca ccgtcaacga cgtggtacag gcggtattga agattcaggc gggctaatcg
    19921 ggtatttcta attatttttc agacggcatg tttttatcgg atgccgtctg aaaacatttg
    19981 tgcggattca aaatgaaacc cggttttttc ggacatattt ttctgaccgt attaagcatt
    20041 gcctatcccg ctttgtggta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    20101 agacagtacg gcaaggcgag gcaacgctgt actggttttt gttaatccac tatattacgg
    20161 cagggaggcg ggctggtttg tctggctggc ggcggcgatg tgcgggttgt ggctgctgcg
    20221 cgccctgact gccaaactgc cccaacagcg ttatgcggct ttgtttatta cactgttttt
    20281 tgccgcagta ttggtgttcg ggcggcatga ttcgatgtat tggtatcccg tcctcgtcaa
    20341 tctgatgatg ttggcggtgt tcggcggcag cctgtttgcc ggacagacgg tcatagaaag
    20401 gctcgcgcgg ctgcaacagc ccgacctgcc tgaaaaagct gtgcgctata cccgccgggt
    20461 aacgcaggtt tggtgcgtat tctttattgc caacgggacg cttgccgctt tgttggcccg
    20521 gttggggcga tatgattggt gggcggttta tacaggcgtg attgcctatg tattgatggg
    20581 gatgctgttt gccggcgaat ggctttaccg caaactcgtt ttgaaagtct gaaaccttat
    20641 gtacacttgc acttcctgcg gcgaaaaaca tgaagatatg cccgcaatcg gctttaccgc
    20701 ccccgatccc tataatcaac tcagtgaaga agaacgcatt gcttatcaag cagaatgcga
    20761 ttctgatttc tgcatcatcc gctatcccga ccaaaccgac cgctttatcc gcgcggtgct
    20821 gccgattccg attatcgggc atgaggaaac gctggaatat ggcgtgtggg tgtccgtcag
    20881 cgaaaagagt tttaacgact atcaaagcca ctttcacgac aatccggaaa acgccgttta
    20941 tttcggcatg atctgcaacc ggctgccctc ttatgaatcc gacaccttcg gtttgcattg
    21001 caatgtcgtt acccaacccg atggtcaacg ccctttgctg caacttcatc aaagcggcgg
    21061 acatccgctg gtacgcgact tttatcacgg catagaatat gccgaagcac aggcaaggat
    21121 aggggcggta tccgaatcgg attgttcagg ataaaaggac aaaaaggata aatatgaaat
    21181 acaaagacct gcgcgacttc atcgtcatgc tcgagcagca aggcaaactc aagcgcgtcg
    21241 cccaccccgt ttccccgcat ttggaaatga ccgaaatcgc cgaccgcgtg ctgcgtgccg
    21301 aagggtcggc gttgttgttt gaaaacccga ttaagcccga cggtacgcgc tacgattatc
    21361 ccgtgttggc aaacctgttc ggcacgcccg aacgtgtggc gatgggcatg ggcgcggaca
    21421 gcgtgtccaa gctgcgtgaa atcgggcaga cgctggcgta tttgaaagaa cccgaaccgc
    21481 ccaaaggcat caaagatgcg ttttccaaac tgccgctact gaaagacatt tggagcatgg
    21541 cgccgaacgt ggtgaaaaac gcgccgtgtc aggaaatcgt gtgggaaggc gaaaacgttg
    21601 atttgtataa acttccgatt cagcattgct ggccggaaga cgttgcgccg ctggtaacat
    21661 ggggcttgac cgtaacgcgc gggccgcaca aaaaacgcca aaatctcggc atttaccgcc
    21721 aacaactcat cggcaaaaac aagctgatta tgcgctggct gtcgcatcgc ggcggcgcgt
    21781 tggattatca ggaattccgc aaactcaatc ctgatacgtc gtatcccgtc tccgttgtgc
    21841 tcggctgcga ccccgccacc attttgggcg cggtaacgcc tgttcccgat accttgagcg
    21901 aataccagtt tgccggactg ctgcgcggtt cgcggacgga actggtgaaa tgtatcggca
    21961 acgatttgca agtgcccgca cgtgccgaaa tcgtactgga aggcgtcatt catccgaacg
    22021 aaaccgcgtt ggaaggccca tacggcgacc acacgggcta ttacaacgag caggattatt
    22081 tccctgtgtt tacggtcgaa cgcattacca tgcgcgaaaa cccgatttac cattcgacct
    22141 acacgggcaa accgcctgat gagcctgccg ttttgggcgt ggcgttgaac gaagtgttcg
    22201 taccgctttt gcaaaagcag ttccccgaaa tcaccgattt ctacctgccg cccgaaggct
    22261 gctcctaccg catggcggtg gtgagcatga aaaaacagta cgccggacac gccaagcgcg
    22321 tgatgatggg ctgctggtcg ttcctgcgcc agtttatgta taccaaattc atcatcgtgg
    22381 tggatgacga tgtgaacgtg cgcgactgga aagaagtcat ctgggcggta accacgcgca
    22441 tggaccccgt gcgcgacacc gttttggtgg acaacacgcc catcgactac ctcgacttcg
    22501 ccagccccgt cagcggactc ggcggcaaaa tgggtttgga tgcgaccaac aaatggccgg
    22561 gagaaaccga ccgcgaatgg gggcgcgtca tcaaaaaaga ccctgcggtt acggcgaaga
    22621 ttgatgggat ttggggggaa ttgggcttgt aggttttacg gcaatgccgt ctgaagcgcg
    22681 agggcgtttc agacggcatt tttgtacgca ggatgtttac ggttctttag gtttgagcca
    22741 ctgccattgt tccagcaggg tgtcttcgag gattttctgt ttctctttgc gcgaattgat
    22801 gccgccggtc agcctgccgt aatcgacggt aatggacggc ttgtccaccg taccggtgat
    22861 tttcagggga atcggtttgt ttttcggatg gacggcgttg cggataagga catcttcaga
    22921 caattcctgc gtatccagat tggtatagcc gttgctggta acatagaggc tgtcggagaa
    22981 gagttcggta tcgatgtggc ggctgatgcc gtctgaaatt tcgctgttga gcgtgaatcg
    23041 gtggaagggt gtgctgggtg cggcattgtc ggcagttttg cccgaaatgc cgtttttcag
    23101 gatattgtcc atgtcgatac cgtgccatgc accgttggaa atatttagcg acaggctgcc
    23161 ctgaagcgag cggataagct cttttcgggt ttcgccgccc gcggtcaggt cgatgaccgc
    23221 gtcgccgttg ccgctgaagc tgtggaagcc gaacaggtct tgcagcagcg gttggatttg
    23281 gatgttgctt gcattctgtt gcaggcggta agtggcggga cgggtgttgg cgatgctgat
    23341 gccgccttcg gtatggccgc cgtaaagccc tgacttgaaa cggctgagcg cgatatggtc
    23401 tttgtcggcg tggaggtagg tttccatatc gtccagttgc aggccgggaa gttggacttt
    23461 tccgattttc aggtgcgcct cgatgtcgcc ggacagcttg gcgagggtgt cgggaaatat
    23521 tttgccgttt tgttgccgca cgtcgtcaag atagggggtc aggttcaatt tttgcagtgc
    23581 gacggcggct tccagatgcg gtgcgtcttc atgtgtgtat ctgaatttcg cggcaacggt
    23641 ttggcggtcg aatgtgccgt ttaattcggc attccaattt tgcagattcg gtacggacag
    23701 cgaaccgtcg agccggctga tgaaacgggg ttgcggcagg cggttgacgg tatcctgaag
    23761 ggtcgataca tacaggcgcg gcgcgtccag ccctttgttt tccgtccata cgagcggcga
    23821 attgagggag aagttggtct ggtggcgcgg tgttttgaag ctgccggaga tttcggcgtt
    23881 gccgatgttg gcgatgccgg agtgcaggtt ggctttgtcg agtttgaacg aaccgtccca
    23941 tcgggcatat tcgccgccga tggtaaatgc gccgttgacg gtttcaattt taatgctgtt
    24001 gttcctgagt gccagcgtag ggatttgggc ggtcaggtgg aggttgcgga aggaagtgtc
    24061 tgcacgcagg ccgaggccgg cggcatccgc tccgccggcg ttgaaacgga cagaagggct
    24121 gccggtggtg gaaatggtga tgccgtgtcc gtccagcgaa gtggaagctt caaaatggaa
    24181 cggtgagatt ttgggcgtgc cgatgccgtc tgaaaggaac agccccctgc ttttccacgg
    24241 gacggacagc tttccccaaa ccagtatgcc cgaactttca aacggctgcc ccgacgaatc
    24301 gggggattgc aggttgaggt tgatttcctt caggataagc tgttcctgca ggaaattgag
    24361 gcggacggtg ctgttttcga cgataatgcg gttgactgag gcttggcgtt tttggctgtc
    24421 gatcaggtct tggatgttcc aaacaccttt cccgtcgcgc gtcagggcaa gttccgcact
    24481 cgaaaccacc catttttcaa tctgtatctg atccgaccac aggtttttcc agctcaatcc
    24541 gattttggtt tcttggacgg aaacggcagt ccggtcgccg ccgggttcgg taatggtcag
    24601 gtttttcagg atgacggtcg gccggggcag gagcctgcgc tgaatgtccg catcaaacga
    24661 gattttccgg tgcgtatgag caatgctttg ttggaggcgg cttctgatgt tttcaggtgt
    24721 gaggatgcga tatacggaag cgtgcagccc gattgccgcc aaaagcagta tcgtcagcac
    24781 ggccaccgca tatttcagac ggtatttgtg gaaaaccgat aataaatcca tagcccgaac
    24841 aatctgtaaa acacgccaca gtataacacg gcttgcaaaa atcctatttt gggcaggccg
    24901 tttcttgtgt caggaggaaa tatgattatt gtcatgagca ggcgtgccgc cgaagcggac
    24961 attgccggcg ttgtcgcctt tatccgcagc agaggtctgc gagaacacat ttctcacggc
    25021 gacgagcgta ctgtcatcgg cgcaatcggc gacgataggg ttttgagcgt cagggaggtg
    25081 cagacgctgc cggaggtcga gaaagccgtc cgcattttgg atacatggaa aaccgtcagt
    25141 cgggaaaatc gggcggaaga cagccgcgtt gccgccaaag gcgtggcttt cggcggcggg
    25201 gaaaccgtcc gtatcgccgc agaaccgtca gtatggcgca atgcggatgc agtgtttctg
    25261 gatccgtttt tcacttcggc aaacttatac gacacttcaa gtgcggatga agggcgggga
    25321 cggtgcagac ggttggcgga acagaccgca tcggcgcatg gcgcagggaa acccgtattg
    25381 gtgcgcgtcc gcaacgtccg ccatgtcgaa gcggctttga atgcgggggc ggacatcctg
    25441 tatttgggcg gcgggctgat gtccgatctt gccgtattga atgaagccgg aagcctgaac
    25501 attcccttgg tgctgtgtaa agacaagcac cattctgccg aagattggtt gaatgccgca
    25561 gaatatgtcg tctcgcgcgg caaccgccac ctgattctgg gcgagtcggg cgtgttgagc
    25621 cataccaaag accatcccta ccgtttggat gtcgaatcca tcgtgaaagt ccgccaaatc
    25681 agccatctgc ccgtgattgc gaatattacg ggattatgga accgcgatat gccgcaggaa
    25741 atcctgtacg gcttggcaaa ggcggcaggc gcgtgcggaa tcgtcggaac ctgttttgaa
    25801 aagtcgggcg gatagcaggc ataccggcag attcaaaggc ggagcagaca ttcggacaac
    25861 atgccgtctg ccgcccgaat agcggtgccg tctgaagcgc acaagacggt ttcagacggc
    25921 atataatctg ttttttattt tgggaggagt tcagatggca cgacacgttt ggcaggcagg
    25981 acggtttgaa atcggtttgg acaagccgaa aatcatgggc atcgtgaatc tcacgcccga
    26041 ttctttttcc gacggcggcg cgtattcgca aaacgcccga acagccttgg cacacgccga
    26101 acagctttta aaagaaggtg cagacattct cgacatcggt ggcgaatcga tgcggccggg
    26161 tgcggcggat tatgtttcgc cggaagaaga atgggcgcgg gttgcgcctg tgttggcgga
    26221 agtagcaggg tggggtgttc ccatcagttt ggacacgcgc cgtacggtga ttatggaaaa
    26281 agcgttggca ctcggtgtcg tcgatattat taatgatgtg gcggcgttga ctgacaaggg
    26341 cgcggtcgaa ttgctggcgc gtcaggcgga cacgggcatt tgcctgatgc acatgcaggg
    26401 tttgcccgaa aacatgcaga ttaatcccaa atatcaagat gtggtcggcg aagtggtgcg
    26461 gtatttgaaa gagagggcgg cggaatgtat cgcggcaggc atcgctccgc aacgcatcac
    26521 gcttgatccg ggtttcggtt tcggcaaaaa cctgcaacac aatatcgcgc tgatgcggca
    26581 cttgcccgaa ttgatggcag aaacgggttt cccgctgctg atcggtgtgt cgcgcaaaag
    26641 catgataggc gagctgaccg gcgaggcgga cgcagcggcg cgcgtacacg gcagcgtggc
    26701 ggcggcgctg gcttccgtag cgcgtggcgc gcaaatcgtg cgggtgcatg atgtgaaggc
    26761 gacggcggat gcgttgaagg cgtgggaagc gttgggcatc aatctgtaaa cagcggttcg
    26821 catacttcat gccgtctgaa aaggacgggg cggcttcaga cggcatcaac ggtgcagaca
    26881 gcagggcaca aacagactgt ataatctcgg acaacggttc acattctcgg agcaacacat
    26941 ggcaaaaaaa tatttcggca cggacggcgt gcgcggcgaa gtcgggcagt ttccgattac
    27001 gcccgatttt gtattgaaac tcggctatgc ggcggggcag gttttggtgc agcacgacac
    27061 cgaccaaaaa ccgaccgtcc tcatcggcaa agacacgcgc atttccggct atatgctgga
    27121 agccgctttg gtggccggtt ttacggcggc gggtgtgaat gtggtacaga ccggaccgct
    27181 gcccacgccc ggcgtggctt atctgacccg cgcgttgcgc ctgtccgccg gcgtgatgat
    27241 ttccgcgtcg cacaacgcct attccgacaa cggcatcaaa ttcttcgccg aaggcggcgt
    27301 gaagctttcc gatgaaatcg agttggaaat cgaagccaaa atcgacgaag aaatgaaaac
    27361 ccagccttct gcacgcctcg ggcgcgcccg ccgcatcagc ggtgcggacg accgctatat
    27421 cgaattttgc aaatccacct ttcccagcca ttccgacctt cggggtttga agttggttat
    27481 cgataccgcc aacggcgcgg ggtacggtgt cgctcccaag gtgtttcacg aactgggcgc
    27541 acaagtcgtc agcatcggcg acgaacccaa cggctacaat atcaatgaaa aatgcggtgc
    27601 gacttatacc aagacactcc aggccgccgt tttacagcat gaagccaact acggcatcgc
    27661 cttagacggc gacggcgacc gcttgatgat ggtcgataaa aacggtaagg tttacgacgg
    27721 cgacagcctg atttacgtca tcgctaaagc ccgcgcccga gaaggtatca acatcggcgg
    27781 cgtggtcggc acggtcatga ccaatatggc gatggaaaac gccctgaaag agcagggcgt
    27841 cgatttctgc cgcgccaaag ttggcgaccg ctatgtgttg gaacagttga accaacgcgg
    27901 ctggctcatc ggcggcgaag ccagcggcca tattttgtgc atggacaaac acaacaccgg
    27961 cgacggcatt atttccgcgc tgcaagtttt ggcggcactg caaacgctga accaagacct
    28021 cgccaccgtt tgcgccgatt ggcagccgta tccgcaaacg atgattaatg tccgtatcgg
    28081 caaagggcaa aactggcagg aagcctcaaa agacgtgttg gcggaagtgg aaaaagaact
    28141 cgaaggcaaa gggcgtgtcg tgttgcgcgc ttcgggaacc gaacccgtcg tgcgtgtgat
    28201 ggtggaggcg cggcaggcgg attgggcgcg cgacggtgcg gaacgtattg cttccgccat
    28261 cggcagcctt taggcaaaat caggcaaaac cgtttcaaca gcggcggttt tcctgtttaa
    28321 atgccgtctg aacgtccggc agagggcgcg aatctgcccc ttgccgcttt cagacggcat
    28381 aatctttgat agaatgccgt ttcgcgcaga aaatgtttcg gcttgttctg actttttatc
    28441 tccgacaatg gaatatccgc tatgtttgcc cttttggaag ccttttttgt cgaatacggc
    28501 tatgcggccg tgtttttcgt tttggtcatc tgcggtttcg gcgtgccgat ccccgaggat
    28561 ttgaccttgg taacaggcgg cgtgatttcg ggtatgggtt ataccaatcc gcatattatg
    28621 tttgcagtcg gtatgctcgg cgtattggtc ggggacggca tcatgttcgc cgccggacga
    28681 atttgggggc agaaaatcct caagttcaaa ccgattgcgc gcatcatgac gccgaaacgt
    28741 tacgcacagg ttcaggaaaa attcgacaaa tacggcaact gggttctgtt tgtcgcccgc
    28801 ttcctgccgg gtttgcggac tgccgttttc gttaccgccg gcatcagccg caaagtatcg
    28861 tatctgcgct ttctgattat ggacgggctt gccgcgctga tttccgtgcc cgtttggatt
    28921 tacttgggcg agtacggcgc gcacaacatc gattggctga tggcgaaaat gcacagcctg
    28981 caatccggca tcttcatcgc attgggcgtg ctggcggcgg cgctggcgtg gttctggtgg
    29041 cgcaaacgcc gacattatca gctttaccgc gcacaattga gcgaaaaacg cgccaaacgc
    29101 aaggcggaaa aggcagcgaa aaaagcggca cagaagcagc agtaaatcag attttcgacc
    29161 gtccggacgc atttcgcgtt tcaggcggtc tttgcatttt caggatgagc catgaaacag
    29221 aaaatttttg tcctttatac cggcggcacc atcggcatga cgcaaagcag cgaaggcctg
    29281 cgccccgata ccgcgctggt cgggcaggca ctcgcgccgt tttcaaacgg catggatttc
    29341 gattggcacg tttgtgggac gctgattgat tcttccgccg tccaactgca aaactggtgc
    29401 gcttggctgg acatcattgc cgccgcgctg ccgcagtacg acggcgtttt ggtgctgcac
    29461 ggtacggaca cgctcgccta taccgccaat ctgttcgccc tcgcgctgca gggtttggac
    29521 aagccgatcg tcctgaccgg ttcccaatgg tcgtacaacg ccgtgggcag cgacgcgccg
    29581 cgcaatctgg cgactgccgt tgccgcattc ccactcggat tgaagcagac gcttattgcc
    29641 tttgacggca aactttatcc cgccgtcggc agcagcaaag tcagcaccga aaccgcctca
    29701 ggcttcgata atccgcattt cggtgtcatc gccgaatggg atgaaactca aggctggtac
    29761 aatcttcgta ttccgtccca agatgcggcg gccgtttcag acggcctcaa aatccgctat
    29821 cctgacccgc aggcaaaaat cgccgtccgc acgcttattc ccggctttgc cgtccaagaa
    29881 cttgccgacg gactcggaca gcttcccgcc cacgccctta tcctgcaaag ttacggacac
    29941 ggcaacacgc ccgcagacga aggtttcatc cgcgccgtgc aagactttac gcagcaaggc
    30001 aaactgctgc tcaacatcag ccaagtccga caaggcagaa ccgctgccgt ttacgcgcaa
    30061 ggcaacgcgt tccgcaactc aggcatcatc aacggcggca aatgcaattt ggaaacagca
    30121 accgcattga tgacgcttgc cgtttccgaa ggatggggcg cggaagatgt tcacaaagaa
    30181 ttagtaagac taaagttagt gtgaaaccgg aatgaacttc accgacaccc actgccacct
    30241 cgccgatccc gccttacgcg aaaacctgcc gcacattttg accgccgcgc ggaaagttgg
    30301 ggtgaagcgg tttatcgttc ccgccacctg tccgcaggat tggcaaagcg tggcggaact
    30361 gtcggaaatg ccgtccgaac acggtcaaat ccgtattgcg ctcggcatcc atccttggtt
    30421 ttcagacggc attgccgagc gtgatttcgt ccgcctggaa gcgatgttgg cacgttatcc
    30481 gcaggcatgg gtcggcgaaa tcggtttgga tttttacgac aaaacccaaa cgccgccgca
    30541 gcgcgaacgg caaatccaag ttttcgtccg ccagcttgcc atcgcgcaaa ccctgcgccg
    30601 ccgcgtgatt atccataatc tcaaagccac cgccgacatc gccgccgccg tcaaacagac
    30661 aggctttgcc caaggcggca tcgttcacgc cttctcaggc agcgcggaag aagcgcgcgt
    30721 gttgacgaaa ctgggtttca aaatcggcat cggttcgttg ttgctcaatc cgaacgcgcg
    30781 taaagtgcgc gatacgctta aagccttgaa cgacggggat ttcgtattgg aaaccgacag
    30841 cccgtttatg ctgaaaaaag agattaatac gcctgccaat attccgggta ttgcaaagat
    30901 tgctgcggaa atccgcggaa cctgtgtgga agagattgcc aaggtaacgg agcgcaatgc
    30961 cgatgccctg ttgggacggt aggttatcca tctgagatta tctttttgaa tgtccggaaa
    31021 acctattata ataattcagt gttgatgccg tctgaaattc aggcggcgtt tgttatggaa
    31081 gaattatgaa gccgtctatc ttagaaaaac tgcaacaact cggcgaccgg ttggaagaag
    31141 ttacccacct tttagggcaa cccgaagcca catccgatat ggacaactac cgcaagctca
    31201 cgcgcgaaca cgctgaactg acccccgtcg tcgaagtatt ccaaaactat caactggcgc
    31261 aaagcgattt ggcggatgcc gaagaaatgt tgtccgaccc cgaaatgaaa gactttgccg
    31321 ccgaagaaat cgaagcggcg aaagccaaaa tcggcgcact cgataccgaa ttgcaaaaac
    31381 tgctgctgcc caaagatgcc gacgacgaca aaaacatctt tatcgaagta cgcgccggaa
    31441 cgggcggcga cgaagccgcg ctgtttgctg gcgatttgct gcgtatgtac agccgctacg
    31501 ccgaacgcaa ccgctggcag gttgaaatcg tgtccgccaa cgaaagcgag ttgggcggct
    31561 ataaagaagt tatcgcccgt attatcggct taggcgcgta cagccgtttg aaatttgaat
    31621 cgggcggaca ccgcgtgcag cgcgtccccg ccaccgaaag ccaaggccgc atccacacct
    31681 ccgcctgcac cgttgccgtc atgcccgaag cggacgaact ggaagacatc gagttgaacc
    31741 ccgtcgacct tcgcatcgac accttccgcg catccggcgc aggcggtcag cacatcaaca
    31801 aaaccgattc cgccgtccgc attacccacc tgcccaccgg catggtggtc gaatgccaag
    31861 acggccgcag ccaacacgcc aacaaagcgc aggcaatgaa agttctcgcc gcccgcctga
    31921 acgacgcgca aaaacgcgaa gcccaagcca aagaagccgc cgaacgcaaa tccctcatcg
    31981 gcagcggcga ccgcagcgaa cgcatccgca cctacaacta tccccaaggc agggtaacag
    32041 accaccgcat caacctcacc ctgcacaagc tggattttgt gatggacggc gatttggcag
    32101 aaatcaccga tgccctgatt gccgaacatc aggcggagct tttggcggca atgggcgatt
    32161 gagttttgtg ttttgaaatg gaaatgccgt gcatgcaatc aattttaatc caccatattc
    32221 tactgatttg tttgaattca gtgtattatg ccgtttctca accatagacg acaactggac
    32281 atattgatga agattgcgat ttacggtacg aaaagctatg accgcgaaca ttttacccgc
    32341 gccaaccggc atttcggctt cgagctggag ttttttgatt ttatgctgga tgcgaaaacg
    32401 gcgaaaatgg cggaaggcgc ggaagcggtt tgtatttttg taaacgacga cggcagccgg
    32461 cccgtgttgg aaaaattggc gcaaattggc gtgaaaactg tggcgttgcg ttgtgccggc
    32521 ttcaataatg tggatttaaa ggcggctgaa gagttgggct tgaaagtcgt gcgcgtgcct
    32581 gcctattcgc ccgaatcggt tgcggaacat acggtcggtc tgatgctgac tttgaaccgc
    32641 cgtatccaca aagcctatca gcgtacccgc gatgcgaatt tttcgctgga aggtctgacc
    32701 ggtttcaata tgtacggcaa aacggcgggc gttatcggca cggggaaaat cggtattgca
    32761 accatgcgga ttttaaaggg tttcggcatg aacctgctgg ctcacgatcc gttttgcaac
    32821 cccgaagcgg aaaaaatcgg cggcaggtat gtggatttgg acgagctgta tgcccagtcg
    32881 gacatcatca cgctgcattg ccccgccacg ccggaaaacc actatatgct gaacgaagcg
    32941 gcgttcgaca aaatgaaaga cggcgtgatg attatcaaca ccagccgggg cgggctgatt
    33001 gacagtgccg ccgcgattga ggcgttgaaa cgccggaaaa tcggcgcatt gggcatggat
    33061 gtgtacgaga acgagcgcga gctgtttttt gaagacaaat ccaacgatgt gattaccgac
    33121 gacgtgttcc gccggttgtc gtcctgccat aacgtgctgt ttaccggtca tcaggcgttt
    33181 ttgacggaag aggcgttggg taatatttcg gaagttacgc tgtccaatat ccgcgaggtc
    33241 ggacagatcg gcgattgcgg caatgcggtt cgtgccgacg gctgacgtgt tttaaaagtt
    33301 taaaggaaat gctgtctgaa ccaggtttca gacggcattt gacgttcagg cagcttgcaa
    33361 acttatttca cttccaactt ggcaacgccg cccatatacg gttgcagtgc ggcagggatg
    33421 ttgatgctgc cgtcggcgtt ttgatggttt tccaatacgg cgaccagcgt tctgccgaca
    33481 gccaagccgg agccgttcaa agtatgtacc aagcggtttt tgccgttttc gtctttgaaa
    33541 cgcgccttca tgcggcgggc ttggaaatct tcgcagttgg agcagcttga gatttcgcgg
    33601 taggtatttt gcgccggcac ccaaacttcc aaatcatacg ttttggttgc gccgaagccc
    33661 atgtcgccgg tacacagggt aatcacgcgg tagggcagtt ccaaagcctt caggatgttt
    33721 tcggcatggc cgaccatttc ttccagcgtt tcgtatgatt tttcgggatg aacgatttga
    33781 accatttcca ctttgtcgaa ctggtgctgg cgaatcagac cgcgcgtgtc tttgccgtac
    33841 gaacccgcct cgctgcggaa acagggcgaa tgcgcggtca gcttcagcgg cagttgttcg
    33901 gacgggataa tgctgccggc aacggtattg gtcagggtaa cttcagctgt cggaatcaga
    33961 tattgcgtgg ttttgctctc gtcgccgcca cgggtaacgt ggaacaaatc ttctgcaaac
    34021 ttcggcagct gacccgtgcc ttgcaaggtg gtgtcgtcca cgatataggg tgtgtaatgc
    34081 tcggtgtagc cgtgtttcag cgtgtgcgta tcgagcatga attgcgccag cgcacggtgc
    34141 aggcgggcga tttgtccgcg catcacggta aatcgtgcgc cggaaagttt cgcgccacct
    34201 tcgaaatcca aacccaaagg ttcgcccaaa tcgacatggt ctttgatttc aaagtcaaat
    34261 tcgcgcgggg tgccgacttt gcggacttcg acgttttcgg tttcgtcttt accggcaggt
    34321 acgctttcgt gcggcaggtt aggtatgctc aacaaccatg cgtccaattc tttttgaacg
    34381 gcatccaaat cggcggcagc ctgttccaaa tcggttttga tttgggcgac ttgatccatc
    34441 gccgcctgcg cttcttcgtg tttgccctga cctttcagtg cgccgatttg tttggaaatg
    34501 ctgttgcgcg aggcttgtag ttcttcagtt ttcacctgaa cggacttgcg tcgttcttcc
    34561 agtgcgtcaa aacgtgcggt atcaaagtca taaccgcgcc gtgcaagccg ttcggcaacg
    34621 gcggcggtgt tgctgcggag caattggata tctaacatgg tctgattccc gaattaattt
    34681 gaaacacatt attgtaaacc aaatttaaga tattttcccg aatccgtccg tgtttcgaca
    34741 tgcttgaacc ataccgtctg atttgtcaaa accgttcgat ttgtgggaaa atcccaagat
    34801 tgaatttaac ggagggcgac accgtgttgg acagagaagg ctatcgcccc aatgtcggta
    34861 tcatcttaac caacaaccgc gacgaagtct tttggggtaa gcgcgtgcgc gaacattctt
    34921 ggcagtttcc gcaaggcggc atcaagccgg gcgaaagccc cgaaaccgcc atgtaccgcg
    34981 agttgtacga agaagtcggg cttttgccgc aacacgtcaa aattatcggc cgcacgcgcg
    35041 actggctgcg ttatgacgtg ccgaacaact gggtacgccg cgaatggcgc ggatcctatc
    35101 gcgggcagaa gcagatttgg tatctcctgc gcctgaccgg ccgagattgc gatgtcaacc
    35161 tgcgcgccac ccgccacccc gaatttgacg gctggcgttg gcatcaatat tgggcgcccg
    35221 ttgacgaagt gattgatttc aaacgcgacg tttatttggg ggcgttgaaa gaactctctt
    35281 cccgcttcct gcgcggtatg gaaagttatg aagactttgc cgcccgccaa ccttccggca
    35341 accggtaaaa caacggaacg caacgctttc aggccgtctg aaagcgttgt ttttcttact
    35401 ttcagattat tcctatggct aaaaacaacc aatacagcga atccagcatc accgtcctca
    35461 aaggtttgga accggtcaaa gaacgtcccg gtatgtacac ccgcaccgac agcccgaccc
    35521 atatctgtca ggaagtgatt gataacgcgg cggacgaggc gttgggcggt ttcgctacgg
    35581 caatcgacgt gcaaattcat gaagacggtt cgctttccgt acgcgacaac ggccgcggta
    35641 ttcccgtcgg tctgcaccct gaagaaggcg tacccgtagt cgaactcgtg ttcacccgtc
    35701 tgcacgcagg cggcaagttc aacaaaaaag acggcggcag cgcgtatgcc ttttcaggcg
    35761 gcctgcacgg cgtgggcgta tccgtgacca acgccctttc cacccgcctt gaggtaacgg
    35821 tcaaacgcga aggcaaaatc caccgtatcg tgtttgccgg cggcgacgtg gtcgaaccgt
    35881 tggcacaagt gggcaaatgc gccgtcaaag acagcggcac cgaagtgcgc gtgtggccgg
    35941 acggaaaata ttttgaaagc ccgaattaca gcattcccga actcgaacgc ctgctgcgtg
    36001 ccaaagccgt gctgctgccg ggtgtgcgcg tttccctgac ccgtccggtc aaaggcgaag
    36061 acgaagcgca cacccaaacc tggcattacc ccgacggcct gaaaagctat ctgaccgacc
    36121 tgattgccga cgcgcaggaa gccgtaccgc tgttctcttg cgaaaactac atttcagacg
    36181 aacacaacgg cgatttcagc atcggcgaag gcgccgcctt tgccctgact tggctggaag
    36241 aaggctcgtg tgccaacgaa agctatgtca acctcatccc caccccgctg ggcggcacgc
    36301 acgaagcggg cttgaaacaa gccgtgttca acgccgtcag caacttcatc aacctgcaca
    36361 acctcttacc gcgcggcgtg aaagtgcaaa gcgacgacgt gttcggcaaa actgcctttg
    36421 tcctctctgc ccgtgtcctc gacccgcagt tccaaggtca gaccaaagac aaactgacca
    36481 accgcgacgc gctgaaactc gttgctaccg tatcgggcga ccctttggaa ctgtggctga
    36541 accaaaacgt ggacttcggc aaaaaaatcg ccgaactcgc catccgtcag gcacaggcgc
    36601 ggatgcgttc ggttaaaaag attgaaaaga aaaaaggcag cggcgtcgcc gtcctgcccg
    36661 gtaagctgac cgactgcgaa agcgaagaca tccgcgaaaa cgaactcttc ctcgtcgaag
    36721 gcgattccgc cggcggctca gccaaactcg cccgcgacaa agccacccaa gccatcctgc
    36781 ccctgcgcgg caaagtgctc aacagctttg aagtccaccc cgaccaactc ttcggcaacg
    36841 ccgaaatcca cgacatttcc gtcgccatcg gcgtcgatcc gcacggtatt aacgaccatc
    36901 ccgatttaag cggcctgcgc tacggcaaaa tcgccatcct gtccgatgcc gacgtggacg
    36961 gctcgcatat tcaagttttg ctgctgacct tattctaccg ccacttcccg aaactggttg
    37021 ccgacggaca catctacgtc gcccagccgc cgctgttccg cgtcgatgtc aacgcacaag
    37081 gcaaaagcaa acctgcccgc aaattctacg ccctcgacca aaacgaactc gacagcattt
    37141 tggagcggct gcaaaaagaa ggcgtcaaag aaaccgccta ttccatcagc cgtttcaagg
    37201 gcttgggcga aatgaacccc gaccagctca aagacaccac catgcaccct gacacccgcc
    37261 gcctgttgca ggtacaaatc ccagaaggtg cagatgacga gacgcgcgac atcttcgtca
    37321 aactgatggg caaaggcgaa gccgccgccc gccgcgcttg gatggaacgc gaaggcgata
    37381 cggcggaatt ggatatttga accgtgattg ccgcatacga aaaaatgccg tctgaaacca
    37441 aatcggtttt cagacggcat ttcgtttggg gatttattct gctgcggaag atccggctgc
    37501 ttcggcggca gcttgggcgg actgctgttg caaagcctgc tgcacgaaag gatggttttg
    37561 cgcgatcgcc tgttcttcgg gggtaacttc gcctttgaaa cggttgttca aatcgaaacg
    37621 tttgccgccg cgcgccaagg ctttcagata acgcggacgg cggcagtggt tggcgaggac
    37681 gcgtgcaatc agtgcggaat cgtattgcgg caaagcggca atcaaatcct gatcaatgcc
    37741 gagcgccaac ggtttgaagc gtttgaatac atcgtatttg ccgtagatgt ggtcggcgat
    37801 catttcggtc tgtttctttt tgctcatagt ttggacggcg gatttcagtg ccgcgcccaa
    37861 agcggtttct tgtgtcattt cggttgtatt cctctaaaca aacccgtgcg tcaaagcggc
    37921 acggcaatgc tcggtattct attataaagt ctgtgttttg gctaaatttt tatttgtttg
    37981 aaagggtaat gtcgggcgta ttaacctgaa catccgcatt ttgcgcgcga tggcgcaggg
    38041 cgtggtcgat taaaaccaat gccagcatag cttctgcaat cggcgcggcg cgcaagccga
    38101 cgcaggggtc gtgcctgccg tgcgtggcga gttcgatggg gttgccgttg atgtcgatac
    38161 tgcggcgcgg ggtggcgatg gagctggtgg gtttgatggc gatattgacg tggatgtctt
    38221 gtccggtgct gatgccgccg aggatgccgc ctgagtggtt ggacaggaag ccttgcgggg
    38281 tcagttcgtc gccgtgttcg ctgccgcgtt ggacaacgct gccaaaacct gcgccgattt
    38341 ccacgccttt gacggcgttg atgcccatca tcgcgtaggc gatttcggca tcgaggcggt
    38401 caaaaacagg ttcgcccaag ccgacaggga cgttggcggc ttcgatatgc agcttcgcgc
    38461 cgacggaatc caaggatttg cgcacgctgt ccatatagtt ttccagctcg gcaatttggc
    38521 tatggttggc ggcaaaaaaa ggattttggg aaatgtgttc gcagccttca aaccggattt
    38581 ctttttcgcc gacttgggtc acgtaggcgg tgatttccgt gccgaatttt tctttcaacc
    38641 attttttggc aacggctccg gcagcaacgc gggcggcggt ttcgcgggcg gaactcctgc
    38701 cgccgccccg gtagtcgcgc gtgccgtatt tgtgccaata ggtatagtcg gcgtggccgg
    38761 ggcggaagct ggtggcgatg ttgccatagt ctttgctgcg ctggtcggta ttgcggatta
    38821 agagggcgat gggtgtgccg gtggttttgc cttcgaatac gccggagagg atttcgactt
    38881 ggtcggcttc gcggcgttgg gtaacgtggc ggctggtgcc gggtttgcgc cggtcgaggt
    38941 caaattggat atccgcttcg cttaattcca agccgggcgg gcagccgtcg atgatgcagc
    39001 ccaaacctgc gccgtggctt tcgccgaacg tggtcacggt gaagagttgt ccgaaagtgt
    39061 tgcctgccat attttccttc tgtcggcggt atgttggaga cggcggattt tagcataaaa
    39121 gttggaggaa aacgggttat atgccggtaa aggggttgcg tgttttgcag tgaatcggct
    39181 tataatccgg tctgattttt caggatagcg atatgagcga ctacacgcag cagcttcaag
    39241 ataaaaaaga ccaccttaaa accctttttg caggtttgga tgttcctgag tgggaagtgt
    39301 atgaatctcc ggacaaacat taccgtatgc gtgccgagtt ccgtatttgg cacgaaggcg
    39361 gtgaaatgtt ttacgccatg tttgaaaaag ggcagaaggc tggcggggca agcctgatcc
    39421 gttgcgaccg cttcgatgct gcttcagagg cagtcaattg cctgatgcct gaattgattg
    39481 ccgttgccgc ccaatctgcc gaactgagaa accgttggta tgccgtcgaa tttctgtcca
    39541 cgctcagcgg ggaaatgctg gttaccatga tttaccacaa aaaactcggc gacgcatggc
    39601 aacaggcggc gcaagcgtta cagcaacagt tggatatttc cgttattggg cggagcaggg
    39661 gacagaaaat agtcttaaaa caggactatg taacggaaac tttgaaggtc ggcaaccggg
    39721 atttccgtta tcggcaaatt gaaggcagtt ttacccaacc gaatgccgcc gtgtgtcaga
    39781 aaatgcttga atgggcctgc ggtgcagcag aaggattgga tggggatttg ctcgaactgt
    39841 attgcggaaa cggcaacttt acgcttcctt tatctgaaaa gtttgaacgt gttttggcaa
    39901 ccgaaatctc caaaacctcc gtcagcgcgg cgcaatggaa tatcgaagcc aaccgcatcg
    39961 gcaacattaa aatcgcccgt ctgtctgctg aagaatttac cgaagcctat accagaaaac
    40021 gtgagtttaa acgccttaag gatggcggta ttgctttgac ggattacgca ttttccacta
    40081 ttttcgtcga tccgccgcgt gccggcatcg atgagcaaac tttgaagctg gtgtcgcagt
    40141 ttgacaacat catctacatt tcctgcaatc ccgaaacgct gcgtgctaat ttggacacct
    40201 tggtggaaac ccatgcggtc gaacgtgccg ccctgttcga ccaatttccg tttacgccgc
    40261 atattgaaag cagcgtactg ttgaaaaaga aaatactttg aaaaatcaag aggtaagaac
    40321 tatgtaccga cacatcgaat actatcccgg cgatccgatt ttgagtttgg tcgaaacctt
    40381 caaaaacgac ccgcgccctg aaaaagtcaa tttgagcata ggcatttatt tcgacgacga
    40441 aggcaaaatg cccgtgttgg aatccgtgag ccgagccgaa accgcccgcg ccgccgcgcc
    40501 tgcgccgtcg ccctacctgc cgatggaagg cttggacact taccgcagcg cggtgcagca
    40561 tttattgttc ggcaaagaca accctgcgct cgcgcaagga cgcatcgcta ccgtgcaaac
    40621 cttgggcggc tcgggcgcgc tcaaagtcgg agcagatttc ctgcaccgct ggtttcctga
    40681 agcgcgcgcc tacgtcagcg acccgacttg ggacaaccat cgcggcattt ttgaaggtgc
    40741 aggtttcgag gttggcactt acccatatta cgaccctgcc actgtcggcg tgaaattcga
    40801 cgaaatgacc gcgtttttca acaccctgcc cgaaaacagc gtcctgatcc tccatccctg
    40861 ctgccacaac ccgaccggcg tggatatgtc ggaacaacaa tgggacgaag tgttgcacat
    40921 catcaaaacg cgcaaactga ttccgtttat ggacattgcc taccaaggat tcggcggcga
    40981 tttggacagc gatgcctacg ccgtccgcaa agcggtggaa atggaattgc cgctgttcgt
    41041 cagcaattcc ttctcgaaaa acctgtcgct ctacggcgag cgcgtcggcg gcctgagcgt
    41101 ggtttgcccg aataaggaag aagcggaatt ggtattcgga cagctcaaat tcactgtccg
    41161 ccgtatctac tccagcccgc ccgcgcacgg cgcgtatatc gccgccgacg tgatgaacag
    41221 ccccgaactt tacgccttgt ggcaaaacga ggtttatatg atgcgcgacc gcatccgtgc
    41281 gatgcggcag aaactttatg atgtcttaac tgcgcaaatc cccgatcgcg atttcgctta
    41341 tttcatcaaa cagcgcggca tgttcggcta cacaggattg agcgtggaac aagtccgcag
    41401 gctgcgcgac gaatttgccg tttacctgct ggattccggc aggatgtgcg tcgccggact
    41461 gaatacgtcg aataccgact atgttgcccg cgcatttgcg gaagtcttga aataagagtt
    41521 tgaagtaagt caaaagaccg tctgaaaaat atttatcttt cagacggtct ctttataatt
    41581 ggcgcaccca acagggatcg aacctgtgac ctccagcttc ggaaactgac actcttccaa
    41641 ctgagctatg ggtgcgtaaa acgtaaggtt aacgtaaatt ctgcaaattg gcaaaggtta
    41701 tcttgcgccg gcaggcggcg tatggcggca taaggagagt tttcttgagg ataaaccatt
    41761 attttcaaag gattgtttgt tttatacaga taccgttggc aaattttttc atattttcaa
    41821 tgatagcgat tggaaagacc ctgtgaattc agtataatcc agcaaaatat tgttaactgc
    41881 gtttaacaca ccaaactata atttacagca caacctaacc tgacggcgag gcctaccaaa
    41941 tgaaacaact ccgcgacaac aaagcccaag gctctgcact gtttaccctt gtgagcggta
    42001 tcgttattgt tattgcagtc ctttatttcc tgattaagct ggcgggcagc ggctcgttcg
    42061 gcgatgtcga tgccactacg gaagcagcaa cgcagacccg tatccagcct gtcggacaat
    42121 tgacgatggg cgacggcatc cccgtcggcg aacgccaagg cgaacagatt ttcggcaaaa
    42181 tctgtatcca atgccacgcg gcggacagca atgtgccgaa cgctccgaaa ctggaacaca
    42241 acggcgattg ggcaccgcgt atcgcgcaag gcttcgatac cttgttccaa cacgcgctga
    42301 acggctttaa cgccatgcct gcaaaaggcg gtgcggcaga cctgaccgat caggaactta
    42361 aacgggcgat tacttacatg gcgaacaaaa gcggcggttc tttcccgaat cctgatgagg
    42421 ctgcgcctgc cgacaatgcc gcttcaggaa cagcttctgc tcctgccgat agtgcagctc
    42481 cggcagaagc gaaggcagaa gacaagggtg cggcagcccc tgcggtcggc gttgacggta
    42541 aaaaagtctt cgaagcaacc tgtcaggtgt gccacggcgg ttcgattccc ggtattcccg
    42601 gcataggcaa aaaagacgat tgggcaccgc gtatcaaaaa aggcaaagaa accttgcaca
    42661 aacacgccct tgaaggcttt aacgcgatgc ctgccaaagg cggcaatgca ggtttgagcg
    42721 atgacgaagt caaagcggct gttgactata tggcaaacca atccggtgca aaattctaaa
    42781 tttagattaa aatttcggat taaataaaaa cggggcaggt atctgctccg tttttttatg
    42841 ggaatttata gtgaattaac aaaaatcagg acaaggcgac gaagccgtag acagtacaaa
    42901 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta
    42961 aggcgaggca acgctgtact ggtttttgtt aatccactat atttgtttgt ccgtgtggaa
    43021 atgacatcaa agggtgtgaa taggcttttc atctttgaaa tctgtctgcc cgatcggatg
    43081 aataaaaagt aggatgataa aaaaaactga tgtatggata ctggtttaac ccatttgttt
    43141 taaagttata gtcgagatac gtgtatcggt gcaacgagtc tgccgtcgac gaaaatgccg
    43201 tctgaaagtg tttcagacgg catttgtctt tggggtttca acggtcagtc ttcataattt
    43261 tccatcggtg ggcagctgca cacgagattg cggtcgccgt acacgtcgtc cacgcggttg
    43321 acgaagggcc agaatttgtg ttcgcgcacg aagggcagcg ggaagacggc ttcttcgcga
    43381 gagtacggat gcgcccattc gccggttacg tcggaggcgg tatgcggagc attcaccagt
    43441 gggttgtcct ctttcggcca ttcgccgtcg atgactttct gcacttcgcg gcgaatggat
    43501 ttcagggcgg cgatgaagcg gtcgagttcg gctttgcttt cgctctcggt cggctcgatc
    43561 atcagcgtac cggcaacagg gaaggaaacc gtcggcgcgt ggaagccgta gtccataagg
    43621 cgtttggcga tgtcggtttc ggtgatgccg ctttcggctt tgagcggacg caaatcgacg
    43681 atgcattcgt gcgcgacgcg gccgtttttg ccggtgtaga gaatcggata gtcttcgcct
    43741 agacgtttgg cgacgtagtt ggcgttgagc aatgcccaac gtgtcgcctg ctccatacct
    43801 tgtttgccca tcatggtcag gtacatccaa gtaatcggca ggatggatgc agaaccatat
    43861 gctgcggcag ccacggcagt ttgatcggca cttgcgctgt gggtgtcggt caaggtatga
    43921 cccggggcaa acggggcgag gtgggctttc agaccgatgg gacccatgcc cggaccgccg
    43981 ccgccgtggg ggatgcagaa ggttttgtgc aggttcatgt gcaacacgtc tgcgccgact
    44041 ttggcaggct gcatgatgcc gatttgggcg ttgaggttgg cgccgtccat gtaaacctgt
    44101 ccgccgtttt cgtggacgat gcggcagatg tcgcggatgc cttcttcgta cacgccgtgg
    44161 gtggacggat aggtaatcat gatggcggac aaagcgtcgc ggtgttgctc ggctttggct
    44221 ttcaaatcgt cgatattgac gttgccgtgt tcgtcggtat cgacaacgac gactttcaag
    44281 cccagcatgg ccgcagtcgc agggttggta ccgtgggcgg atttgggaat cagacagatg
    44341 ttgcggtgtc cctcgccgtt ggcttcctga tagcggcgga tggagagcat accgctgtat
    44401 tcgccctgcg cgccggagtt gggctggaag gaaatcgcgt caaagccggt gatggctttc
    44461 aggctgtttt ccatgtcggc aagcaattcg cggtagccgg cggtttgcgc ttcgggagcg
    44521 taagggtgga tgtcggagaa ctccgcccaa gtaatcggca acatttccgc agtggcattg
    44581 agtttcatgg tgcaactgcc cagcgaaatc atactgcggt tcatcgccaa atcgcggtct
    44641 tcgagttttt tcaggtagcg caacatttcg tgttcggtgt ggtaactgtt gaacacagga
    44701 tgttgcagaa tgtcgttttg acgcagtaat tcggcgttca gacggccttt gacatcatcg
    44761 gcaaatgcga acgtatcctt gccggtaaac gcgcggtaca aatcaaccaa atcttcgcgt
    44821 gtcgaagttt catggaaggc tgctgcgact tgagtatcgt tgacgcggcg caggttgtaa
    44881 cccgattcca aagcagcggc aaacacttgg tctgttttct ctttactgcc gaaatcgacg
    44941 gtaacggtat cgaagaagac tttgtgaacc actttaatgc cgtctgaaac cagcgcgtcg
    45001 gcaaaggcag aagccagcgc gtgaatgcgc ttggcaatac gtttcacacc ttcggggccg
    45061 tggtaaacgg catacatacc cgccaagttc gccagcaatg cctgtgccgt acaaatattg
    45121 gatgtcgctt tttcgcggcg gatgtgttgc tcgcgggtgg acaacgccat gcgcaaggca
    45181 ggtttgcccg atgcgtcttt ggatacgccg atgatgcggc ccggggcgga acgtttgaac
    45241 gcgtctttaa acgcgaaata agcagcgtgc ggaccaccga agcccatcgg tacgccgaag
    45301 cgttgcgtgt tgcccaaagc aatatccgcg cccaattcgg caggcggttt cagcaaaacc
    45361 aagctcataa tgtcggcgga aacggcaacg attgtgcctt ttgctttcag acggccgata
    45421 acgtcctgca agtcttgcac atcgccgtct ttgccgacgt attggaacag cgcgccgaag
    45481 tattcgccct cgtcggcttg agcaaaatcg ccgaccacca gctcgaagcc gaaatacttg
    45541 gcacgggttt tcatcacgtc caaagtttgc ggatacacgc gctcgtccac aaagaaacgc
    45601 tcggatttta ccttgcccac gcggtgcgcc atcgccatcg cttcggcggc ggcggtcgct
    45661 tcgtccagca aagacgcgcc cgccacaggg aaaccggtca aatcgataca cacttgttgg
    45721 aagttcaaca aagcttccaa acgaccctgc gcgatttccg cctgatacgg cgtgtaggcg
    45781 gtgtaccaac ccggattttc caatacgtta cgcaaaatca cgttcggcac gcgggtcggg
    45841 taatagccta aaccgatata ggatttgttg atcatgtttt tcgacgcaat gcctttcaat
    45901 tttgccaacg cgtccgcttc ggtcagggca tcgggcaaat cgagttcaga cggcatacgg
    45961 atgctttgcg gcacggtgtt gccgacaaaa tcgtccatac ttttctcgcc gacagcggca
    46021 agcaacgccg cttcgtcgcc aaaactcaaa tgccgcgcgg caaattcgtc ggggttgaac
    46081 agttcggaca gtttcatttc tactccttcg ggtttgtagg gctgccatca ggcagaattt
    46141 ttagacatcg gatgattcgg gcaagggtag cacaaaagac gggataatgg cggacaatgc
    46201 gccagaatgc agcaggtcag gacaagcagc accgcaagcg gcagcgataa gggaaagccg
    46261 ccgaccaaaa ccatgaaacc tgcaggcacc agccaagcta agggatgcgc cggaagcgga
    46321 aaaacaatat tgagcagaac caaaatcacg acaaacagcg gcagcaccgc catcaggttg
    46381 gcacacctgt atgttgcgta cgggtcggac gcttcttcag gttcgcaata ttccatgatt
    46441 tcagcctgtc cgggctttat ccacagcagg ctcggacggt cggtcatgcc gtctgaaccg
    46501 gcaggccgtc tttttcaaaa aatgccatac cgctgcaact tcatgcggct gtatggcgta
    46561 ggttttgcac ttcagacggc atatgcgtcc cgaagcagtt tggtgtccca gtcggttcat
    46621 gattatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatttgt
    46681 actgtttgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat ataaacaact
    46741 tgggtgcgga taaacctttg tttatctaaa cccctgtttt ctcaaaaatt gattcaaatc
    46801 ttcggcaaat ttccgatagc ctttgccgtt ggcgtggatt tggtcggatt tcagattatt
    46861 atcgcccaaa atttccgccc acgcgccgcc gaacagcgga atgccgtatt cctcggacaa
    46921 atcctcatac agcggatgat cgctcaaatg cccgaacaac gcgcccaagg taatgtgcgg
    46981 cacgccgacg aggacggcgg ggatgttttc cttctgcacg gtttcgatga ttttcgcgat
    47041 attggcgcgg gtctgctcct tgggaacttt gcgcagaaag tcgttgccgc ctatgccgac
    47101 aatcacaagc ttgggtttgc gtgccaacag cgcgggcagg cgcgacaggg cttgggcgga
    47161 tgtatcgccc gatacgccgc cgttgacaat attccaaccc gtcagttttt gcagttgcgc
    47221 ggggtaggat tcgccggggt ttgcgccgta gccgaaggta agcgaatcgc ccaaggcaag
    47281 tacggtgctt ccttcgggaa ttttggcgtg ggttcgggcg gattttctgc cgcaggcggt
    47341 aaggagcaac gcgcctgcgc cgaggaggaa ggttcttctg ttcattggtt tttcagatgg
    47401 catcgagcca tttcgggtgg tatttcaggg tttcacggta aaccggacag ctttcggcgt
    47461 gcgcgccttt gaggtagccg gtactcatca gaaattcgcc gacgatttcg ccgccgacga
    47521 atttgaaatg ttttttaaag agtttaaccc attcgtcttt gcttcgcgga tggtgcgcgt
    47581 cgagccagtt cttgaacgag ccgtattctt gttgcaacgc ttggatttgc cgtgcattga
    47641 aaatggcggc atcgattttc aggcggttgc ggacaatacc cgcatcggca agcagccgct
    47701 caatatcggc ttcgccaaat gcggcaacag tatcgatgtc gaacccttca aatgccgtct
    47761 gaaacgcctg ccgcttcttc agcatcagcg tccagctcaa tcctgcctga ttgatttcca
    47821 acaccagccg ctcaaacaat tcattgtcgt cctcaatcgg aaaaccgtat tgggtgtcgt
    47881 ggtaatgttt gttcgggttg tcggtgtttt cgggaagtga ggcggcaaat tcgcaatagt
    47941 tcacggttta ctcgaccatc tgttgcgccg cctgtttcag tccctgttcg agtgcggcgg
    48001 tcatggcggc gtagccgtca ccctgctgtt cggtttcgat atggaagggt ctgttcgtac
    48061 cgtcgggtag gacggcgtag ccgctgatga gggttttgcc cgtgtagctg ccttggaatg
    48121 cgtcgatata gaccgtccat ttttcggtac tgccgctgcg tgaggcagga acaaagatgc
    48181 gtgtgctgtc caaacggttg aatgcattgc tcaacgccgc ttcgagcata tcgtccaagg
    48241 tgtctgccca gacgtggttt tgtgcggtgt tgaggcggta ggggtcggtt tgatagacca
    48301 gtccgccgcg tttgagcggt tcggcaagac ggacttcgac ggcagtttcg ccgccttgcg
    48361 ttgcaggacg gatgtagcgg ctgtcgggca acacgaaata ttgtgtgctt tgcacagtac
    48421 cgcaggcggc aagcgtcagg gcggcggcaa tcgggaagag gcgcattatc ggcttccttt
    48481 cgggataggg tctttgctgc tgctgttgaa aatcagcgcg ttgggttttt ctttcaaagt
    48541 gttaatgacg ggttgaacgt cttttaaggt tttgtccaaa ctttgcagcg tattttgtac
    48601 gtcgccgtag ataggcgatt gaggcgatac gccttgcagg gttgtgcgca actctttcag
    48661 ggtttggttc agttcgttcg gaatgttttg tgtctgcggt ttgccgacca gtttgtcgat
    48721 ggagcttagg gcggcattgg cagatttgag tgtggatttg agctcggcaa gcgaaccgtt
    48781 caattcggca accgtcttat ctaaaggcag tttgtcgaac ttgtccagca aatccgccaa
    48841 tttgacctgc aaatcgtcca aaccgccgcc ctgggtcgcg ataacggtat cgcctgcata
    48901 aacggtatgc ggtcgcagct taggtgatgc ggaaggctga tcgttcaact caatcatttt
    48961 gcttccggtc agcaggttgt tgctggagat ggtggcggtc aggcctttgt ttaaggccgt
    49021 ctgaaattgt tgtttccaat gttctttgct ttgttcgtcg gcattgattt ccaaacggga
    49081 aggttcaatg cggatgcgta cgggtatcca gccgttttca aacaggtgca ggctgtcgtt
    49141 gcggtcgaaa taaggaacgt cggaaaccac gccgacattc agccctttgt actcgacggg
    49201 cgaaccgacg gtcaggccgc gcacggattg tttgaaaaac gcggtgtagt acagcgagcg
    49261 gtcgtcaggc aggttggcga cttcgctgcg gctgtcgtaa agcgtgaagc tgtcttcgct
    49321 tttgacgttt ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc ccgacagcag
    49381 ggcaggcaga ggggcggaat tgagtttgat gccgctgcct gtggtttcga tattgatgcc
    49441 gctttccagc caaaaacggc tggcggaatg aatcagtttg tcgttggggc tttggatgaa
    49501 gatggtgtaa tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga cttgcccgac
    49561 cataaagttt tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt ttttaccaat
    49621 caaattcaag cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt cctgcacctg
    49681 gaacacgtct tttgcctcgt cgcttttgcc gggtgtaaag gcgatgtacg aacccgaaag
    49741 cagcgtaccc aaaccggtta cgccgctttg gtcgatacgc ggcttgacca cccaaaactg
    49801 ggtatcgctg cggatgaggt cggatacgtc cgcattgagt tgggcggtta cttccacgcc
    49861 tttttggtcg tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc tcaatacttt
    49921 gatgaccgta ttgttgacct caatgccttc cgcgctgtcc atcaagagcg taaccacagg
    49981 ccccctgttg cggatttcct taacccaaag ccagccgccg gcaatcagcg cgatcagcgg
    50041 aaccagccag acggcggaga gcaaggtgtt gtttttgcgg acgcgtgctt gggcgtgtcc
    50101 gtttggagga gggctgttgt cagtcatgtt tttccgtttc gttaaaagcc atgccgtctg
    50161 aagcgcgttt gtcccaaagc aggcgcgggt cgaaataata ggcggacagc atcgtcagaa
    50221 tcacgaccag gcagaaatag actgccgcac tgcccggaat gacgcgcgcg gcataagtgt
    50281 ggaacgaaca catcaaaata ataatcacaa aaatatcaat catcgaccag cgaccgactg
    50341 cttcggtgat gcggtagagg tgcgacaatt tctttgcgcc cgccggcaaa gcgaagcggg
    50401 cggacgcaat caaaaccgac attgccgcaa tcttcagcac cggcaccaaa atactcgcgc
    50461 tgaaaataac cgccgcaatc agcctgtcgc cctcgtccca catataagcg atgccgttaa
    50521 ggatggtatt gacctccgtg gcggcaggat tggatgaaat cataatcggc aggatattgg
    50581 cagggaaata caaaataacc gccgccgtca gaaacgccga cgaaatactc agacttttcg
    50641 gccgtcggcg gtacagttcc gcaccgcaca cgccgcaggg ggattcggca ccgtctcgga
    50701 aatacaggca gcggctgcaa caggttttgc cttctgatgc cgtctgaacc gcattatccc
    50761 ccgtcagccg cccgatttga aaatacaccc aatgctgggg aaccgatacc gaagtccgaa
    50821 tcagcataac cgacagcgcg aacatcagat aaaacgccga cccgaagcga acctctgcca
    50881 cagacgagag cttgatatac gccaccagcg tggaaacaaa aaacacatcc accatcatcg
    50941 cctgcctcaa gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac acccgtttcc
    51001 gtatcagcgc ggcatagaca tacaggcaca gcagcagaaa cagaaccggc gcgccgaagg
    51061 tcagcacaaa catcacttcg gccaaaaaac cataatcctg aaacaccatc aggcgcatca
    51121 tctcgggcag cgaaaggacg gatgccgcac ccggtatccc gacctcgata tacgtcatac
    51181 cgtaagcaaa cgccatcaaa atcagcgaag ccgccgcata agcgggcggg gcggaaaaag
    51241 gatgcctgcc caccctgaag agcttatagc cgcagcgcgg gcagaacgcc gcttctccac
    51301 tgtccaaccg gggtacatcc gcgcggcagc cgcattccgg acaatctacc gtatgcggcg
    51361 gtagggcttc gtcgcggcag ttgccgcgaa ggctgtgggg gatattttcg gcaaacggct
    51421 tcatcgggaa agtggcaaat ataagtggcg gcattataaa ggaaaaaagg cgatacaccc
    51481 aatcaggatg caaaaccggc aggttcggca gtatgaacaa acccttgtcg tccggcaaaa
    51541 atattttttc gagcggctgt ccgccgactg ccgaacccat ttgataaaat cccgattctg
    51601 taaagaatag taaaatacct gcttaaaaac atcacgaaaa tgatattttt tccaaaaata
    51661 tgtgataata aatttcgttt atgattcttt attattttta tagtggatta acaaaaatca
    51721 ggacaaggcg gcgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
    51781 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg
    51841 ttaatccact atatatgcgg gcaagtgcgg attttgcccg ccccgcatcc ttatccctta
    51901 ccgacaaagg aaaacttatg agtgaaaccg aaaatcaagc cctgacgttt gccaaacgtc
    51961 tgaaggcgga taccacggcg gttcacgaca gcgtggataa cctcgttatg tctgtccaac
    52021 cgtttgtcag caaagaaaac tacattaaat ttttgaaact ccaatccgtt ttccacaagg
    52081 ccgtcgacca catctataaa gatgccgaat taaacaaagc cattcccgag ctggaatata
    52141 tggcgcgata cgatgccgta acgcaagact tgaaagactt gggcgaagag ccttacaaat
    52201 tcgacaaaaa attgccacac gaaaccggca acaaagcaat cggctggctt tattgcgccg
    52261 aaggttccaa tttgggcgcg gcgtttttgt tcaaacacgc ccaaaaactc gattacaacg
    52321 gcgaacacgg cgcgcgccac ctcgctcccc atcccgacgg tcgcggcaaa cactggcgcg
    52381 ccttcgtcga gcatctgaac gctttgaacc tgactcccga agccgaagcg gaagccatcc
    52441 aaggcgcgcg cgaagccttt gcattttaca aagtcgtgtt gcgcgaaacc tttggcttgg
    52501 ccgctgatgc cgaagcgccg gaaggaatga tgccgcacag gcattgagta atgcaaaaat
    52561 caggtatcgg tgtttttatg gagttgcgct ttatatgata cgcagctgtt tgccggaaca
    52621 tatttgcaac cgactatgaa aacttgaaat tttttttcaa taaatttatg aataagcatc
    52681 atgccttttc gtaaaagagg atgaacttat ttttgttatc catgtcatta ggagaatgtt
    52741 atgtcgaatt cgtcatttaa attgagtgca cttgccatcg cctgcggtct agctctttca
    52801 gcttgtagtt cgggaggcgg agacgcgcca agtgttaagc ctaccccgac tgttccgggt
    52861 gcatcaaacc cggcgcctgc caatcccgaa ccttcagcac cggctgcctc accattgaaa
    52921 accggtgagt tgggtgaaaa atatttttac ggggaaggtg aaatcacgaa gctggtcatc
    52981 aatggcgaaa ctattaattt gacagtggct gatgaacagc tgtcaaggag ggtgaagctt
    53041 tggacagact tcgataagat tattacttcc caaggttttg aaaatcttat ttatggtgcg
    53101 gctaaattgg cagatggttc atacgcactc tttgtgcaag gaaagttgag cgacagtctg
    53161 cccggcgata cggttactta tagcggcgat gtattgcatt tccgttcgcg caacttgacc
    53221 gaaggtaatt ggtttaatga aggtaattca ttccgtgcag ccggttcgtt tactgctact
    53281 gtcaattttg ccggtaaatc cctgaacggt aagattaaca gtggtgacgg attgtatatg
    53341 ggtacctagg aattcgaggg aagtcttgat ggcaaccgct ttaccggtac ttggaaaaac
    53401 ggtgagaaag gtagtatctc tggaggattc tatggtgcca aggctgcaga aatggcaggc
    53461 aggtacagct atgttaagga tgaaccaaaa caggccaaga catatacaaa taacagcttc
    53521 ggcgtatttg gtggtaagaa aacccaataa tcccgtttga atccttgatt tgacgaaagc
    53581 cccggtgcgg atagaccggg gcttttgttt gatattatat ggttaggatg acggaaagtg
    53641 ataatatagt gaattaaatt taaaccagta cagcgttggc tcgtcttgct gtactatttg
    53701 tactgtctgc ggctcgccgc cttgtcctga tttttgttaa tccactatat atccgtatac
    53761 cttgctgcta cagtgttaca gtcacagccg gagtgggatg ttgaaggaat tgccggtaaa
    53821 gcctgaaaaa atgccgtctg aaaacgggtt tcagacggca ttttttgatt gccgatatgt
    53881 taacggacga cattggcgcg catgacgacg cggacgtgga acgagcctgc ctgacagtcg
    53941 tattgcccgc cttgcagcgg ttgcttgtca aagtagctgt ggaagccggt aacgccgcgt
    54001 ccgccgagtt tgcctgcggt ctgttggaat ttgcgggcgg cattgattag ggcgcgttcg
    54061 taggcttctg agtcggattt gcctatgccg tttgccgcct gacgggtaac tagattcctg
    54121 ccgtttgcaa ccggtacgcc cccgccgccg aagctgacgg gaatccctga ttccaatacg
    54181 ccgtctgcaa tggctgcatt tattgccgcc tgaccgttgc agcaacggta aatgatgttt
    54241 ttgccgcttc ctgcggtgtc gccccggatg actttgcatg attctccggc cggtgtgccg
    54301 ggggatgccg tgtgcgcgtt tttaggtgcg ctgccggatt ggcaggctgc cagcatcagg
    54361 gcggcggcgg agagtatgag gatttttttt catgtcggat atccttgctt gcggtgggaa
    54421 atcattgcca ttgtaccgta agattcaaca aaatgccgtc tgaaaacggg gttcggacgg
    54481 cattgtggct caggggttca atacgcgtgc gatgagggct ttttctttgc cccgcatatt
    54541 ggggattttg acctggatgc cgttgccggg tgcaacatcg acaggctggc cttttcgggc
    54601 catttgttcc aatttgatgg tttggttgcc gctcgggtgg atgatttcga gcgaatcgcc
    54661 gatggcaaag cggtttttaa cttccaccgt tgcccaacct tcagcgtcga tttcggtaac
    54721 gtggccgaca tattggcttt gtttggcgat ggagtggccg gtcaggtagt tttgatagtc
    54781 ttgggtttgg tggcgttcga ggaagccgct ggtgtagccg cggttggcga ggccttcgag
    54841 ttcgctcaac aggctgtaat caaacggacg gcctgcaacg gcatcgtcaa tcgctttgcg
    54901 gtaggattga gccacgcgtg caacgtagta gagcgacttg gtacggcctt cgactttgag
    54961 gctgtccacg ccgattttgg cgagtttctc aaccacttca atactgcgaa ggtctttgga
    55021 gttcatgatg taggtgccgt gttcgtcttc cataatcggc atcatctcgc ccgggcggtt
    55081 ggactcttca atcaggaaaa ctttgtcggc gtagggatgg cgtttttgac cgttgatgcc
    55141 ttcaaagttt tggttggctt cttcttgggc tttttcaaag ttgaaacctt gcagaagctg
    55201 ggcatcgccc gcatcgcttt ccgtagcgtt gtggactttg tagtcccaac ggcaggagtt
    55261 ggtgcaggtg ccttggttgg ggtcgcggtg gttgaagtag ccggagagta ggcaacggcc
    55321 ggagtaggcg atgcacaatg cgccgtgaat gaagacttcg agttcgatgt cggggcattg
    55381 ttggcggatt tcggcgattt cttccatact caattcgcgc gacaggatga tgcgttcgac
    55441 gccgatattc tgccagaatt tcacgcccca gtagttggtg gtgttggctt gtacggacag
    55501 gtggatcggc atttccggcc atttttcgcg cacggtcata atcaaacccg gatccgccat
    55561 aatcagcgcg tcgggcttca tcgcaatcaa gggctccata tcggagacga aggttttgag
    55621 tttggagttg tgcggcagcg tattgacggt aaggaagaat tttttgttgc gctcgtgcgc
    55681 ttctttaatg ccttgttcga gaacgtcgag tttggcaaat tcgttgttgc gggcgcgcag
    55741 tgagtaacgc gggctgccgg cgtaaacggc atctgcgccg taatcgtagg cggcgcgcat
    55801 tctttccaat ccgccggcgg gcaataagag ttcgggtgct ttcatcgtgt ttccttttcg
    55861 gttgaaaccc cgccctttag ggcggtagaa tcagacttta tttgggaggg gtgtaaccct
    55921 ttccgaatca gggcaacata tagggcgacg ctttatgtgt cgtcctgtgt gttgaaacat
    55981 gatttgtgtg ctttgtaaaa atgtttcaga cggcattgct gcaaatgccg tctgaaaagg
    56041 gtttgaggat gggcggatta tccgcctttt cgtgttcgcg gtcaattttg ccgggaaatt
    56101 tataaggatt tatcaagtat ttgcctgatt gggatacaat gcggcagttt gaatgcgaga
    56161 gagaatgtga tgaatcctaa aatcgtgttt ttcgatattg acgatacgct gtaccgcaaa
    56221 tatacggata ccttgcgtcc ttccgtgaaa acggcggtgg cggctttgcg cggcaaaggc
    56281 atattgacgg cgttggcaac ggggcggtct ttggcgacga ttcccgaaaa ggtcagggac
    56341 atgatggcgg aaacgggtat ggatgccgtg gtaacgataa acggacagtt tgcgctgctg
    56401 cacggtaaaa ctgtgcgcga agtaccgatg gatgccggtt tgatgggcag ggtttgcgcg
    56461 catttggatg gcttgggcat ggattatgcg tttgtcggcg gagaggggat cgccgtgtcc
    56521 gcgctgtcgg aatgcgtgtg ccgcgccttg cagcatatcg ccagcgattt ttttgccgat
    56581 aaggattatt tttcaagcaa accggtgtat cagatgctgg tgtttgcgga ggaaaacgaa
    56641 atgccgccct ggtcggatat tgtggaacgg gaaggcttga aaacggtgcg ctggcacgag
    56701 gaagcggtcg atctgctgcc tgcgggaatg tcgaaaacag acggcatcag aagcgtggtt
    56761 ggggcattgg gtttggaaat ggcagacgtg atggcgttcg gcgacggttt gaacgatgtg
    56821 gaaatgctgt cagaagtcgg gttcggcgtg gcaatgggca acggggaaca ggcggcgaaa
    56881 gaggcggcga aatatgtttg ccccagcgtt gatgaggacg gtgtgttgag gggcttgcaa
    56941 gatttgggcg tgatttgaac gcataataac aaccctgccg gtttcagacg gcagggtcgg
    57001 ttttcagccc ttcatacagc cttcgttttg aagcagggta aataagggcg cgccgcttgc
    57061 acggatattc ttgccgcctt gaaggtcggt aaattccaaa atggcggcgg cttcgacaat
    57121 ttccccgccg agtttgcgga tcagctccag tccggcaagc atcgtgccgc ccgtggcaac
    57181 caaatcatcg accagcagca cgcgcgaacc gggtttgacg gcatcggtgt ggatttccac
    57241 cgcagcttcc ccgtattcga gcgcgtagct ttgcgatacg gtttcaaaag gcagcttgcc
    57301 ttttttgcgg atggggacga aaccgacgtt gagctggtag gcgagtgccg cgccgataat
    57361 gaagccgcgc gcgtccaaac cggcaacgat gtcgattttc tgatccatat agcggtaaac
    57421 caataaatca accaaaagtc ggaagtattc cgcgctttgc aggacgggcg tgatgtcgtg
    57481 gaataagatg cctttttgcg gccagttttc gattttgcgg attttgtcgg caagcgcgcc
    57541 gacactcata gcttcgggat gaaccagcat tgcgtgttcc aagtttgatg tttttaacgc
    57601 catattgtag ccgtcagcct gccgcttgcc aaatcaatca aaacaatagc ggcaaaatat
    57661 gggcatcggc aaataggaaa ccgagtgtcc gttgtgatac aatcggctgg aaaatataca
    57721 ggtaaaaaat atgtccgctt atcgaaaagg caacatcttt atcatttcgg ccgcttccgg
    57781 cacgggcaaa accacgctgg tgtcgcggct gttggcaaac cataacggtt tgcgcgtttc
    57841 cgtatcgcac acgacgcgtc cgccgcgtga aggcgaagta aacggcgtac attatcattt
    57901 tgtttctaaa gaagagtttg agtcgcttat cgcgcaggaa gcttttttgg aatacgcaga
    57961 cgtgttcggc aactattacg gcacaagcgc ggagggcgtg aatgctttgg cggcggcagg
    58021 ctatgacgta attttggaaa tcgacgttca gggcgcggcg caggttcgca acgcgctgcc
    58081 ggaagccgtc ggcatcttta tcctgccgcc gtctttcgat gtgcttgctg cacgtctgaa
    58141 cggacgcggg acggacagtc gggaagttat ccaaaggagg ctgtcgaagg caaggcatga
    58201 aatcgaacag tccgtattgt tcgattttgt cgtggtcaat gatgacttgg cgcgggcgga
    58261 ggaggatttg cgccatattg tgaatgcctg ccgtctgaaa aggtcgcggc aactgggatt
    58321 tattgcagat ttgttggaaa attcctaaaa aaccgcgaaa atacccagtt tcctatttta
    58381 aatatttttg aaagaaagca aataatatgg cacgtattac caccgaagac tgtaccggaa
    58441 aaatttccaa ccattttgac ctgacattgg tagcggcccg tcgggcccgc cagcttgaaa
    58501 atggcaatac gccgcttgtg gacgatgtcc gcaataacaa accgaccgtt accgccttaa
    58561 gggaaatcgc cgccgggcat atcggtacgg aactgttgac gcgcaataaa taaattctgc
    58621 cggaaacgca cgtcggcaca ttctgccgcc gtgcagtccg acggtttgaa acgaaaaata
    58681 aaatatgttc cgaaagccgt ctgaaatgcc tgtccctcaa cccaccgcac cttacgaccc
    58741 cctgaccgcc gaagcgcgtg ccctgctttt ccataccgcc tcctacctca agcccgagga
    58801 acaggcggag cttgaaaaag ctgtcgccta tgcgtttcgc gcccacgacg ggcaaacccg
    58861 caaaagcggg gagccctaca tcacgcatcc gattgccgtt gcgacgcagc tcgccctttg
    58921 gcatatggac atacagggtc tttgtgcagg cgtgatgcac gacgtattgg aagatacggg
    58981 cgtgacaaaa ggggaaatgg cggcggtgtt cggcaatacg gttgccgaga tggtggacgg
    59041 gctgtccaaa ctcgaaaaac tcaaatttga agatcatgca gagcatcagg cggagagttt
    59101 ccgcaaactg attttggcaa tgaccaaaga tgtacgcgtc atcgtcgtca aacttgccga
    59161 ccgtctgcat aatatgcgga cactcggttc gatgcgcccg gacaaacgcc gccggattgc
    59221 aagggaaacc cttgaaatct atgcacagat tgccaaccgc ataggtttga ataacgcata
    59281 tcaagagctt caggatttat cgttccaaaa cctgcatccc aaccgctacg agactttaaa
    59341 aaaagcgatg gacaagagcc ggaagaaccg gcaggacgtt gtcggcaaag tcttgcgcgc
    59401 attcggccag cggctggtag gcgcgaatat agaggccaaa atcaaaggca gggaaaaaaa
    59461 cctgtacggc atccatcaga aaatgatggc gaaaaagctg cgctttgccg aggttatgga
    59521 tatttacggt ttccgcgtca ttgtcaacag cattccagcc tgttatgccg cactcggcgc
    59581 attgcacacc ctctatcagc ccaagcccgg gcggttcaaa gactatatcg ccattccgaa
    59641 aagcaacggg tatcaaagtc tgcatacgac tttggtcggt ccttacggct tgccgattga
    59701 agttcagata cgtaccaagg aaatggatgc tgttgccgaa ggtggaatcg ccggacattg
    59761 gagctataaa tcatattcta agacggtcga tcaggcggtg cttcacacaa accggtggct
    59821 gaaaaatatc ttagatttgc aggcaagcag tgccaatgcc attgagtttc tcgaacacgt
    59881 caaagtcgat ttgtttccga acgaaatcta catccttacg ccaaaaggaa aaatcctaac
    59941 tttgcccaaa ggggcaacgc ctgtcgattt tgcttatgcg gtgcataccg atatcgggca
    60001 caaaaccgtt gccgcacgta tcaacaatat catgatgccg ttgcgtacga agctcaaaac
    60061 cggtgattcc gtcgaaatta ttacatccga acacgccaaa cccaatcccg cgtggttgaa
    60121 tttcgccgtg tcaggcaggg cgcgcagtgc catacgccaa tatattaaaa accttaaccg
    60181 gcacgatgcg gtcgttttgg gagagaacct cttacaaaaa gccctgtcca gtttgctgcc
    60241 caaagatgtc ctgctttcag acggcatcaa agaaaaatac cttgccgatc tcaacgataa
    60301 acagacttca tttgaggaag tgctgtacaa cgtcggtatg gggcataccc tgcctgttta
    60361 tgtcgctatg catattgccg aattggcagg aaagcatttc ggtagtgagg tcaggctcag
    60421 ttccattaaa gtcgatgggc aggaaagcgg gcatattcat tttgcagagt gctgccaccc
    60481 tgttcccggc gattccatcc gtttgctgtt ggttaaggga aaaggcatga ttatccacag
    60541 agatacctgc ccgacgttgt tgaagtccga tcccgaacag cagctggatg cagactggga
    60601 aaatatgaac gggcagaact accgtgtcgg gcttcaagtc caatcggaag acagccacgg
    60661 cctgttggca ttaatggcgc aagcgatttc cgattccggt gcggacattg agtcggtcga
    60721 aacaccgtct aaatcccagt cgggaacgga aggttttgtc gaattcaaat tcttattgaa
    60781 agtcaagaat ctgaatcaat tgaatcagat tattcaaaat ctgcatagta ttccatatat
    60841 ccgcaaagtc atcagaagtt gaagcaggta aaacgggttg ccttttgatt aacaggcaac
    60901 ccgctttatg gtttatccgt tcaattgctt atttgttcaa atatccggca agcctttcaa
    60961 caatctgcat cgccgcttca tctttacttg tttcgggaaa agatagttcc ccgtcgtccc
    61021 cgataatggt aatccggttg gtcggtttgc ccatcgcgat tgaaacatca ttggcaacta
    61081 tcaccggtat ttttttctta atacgttttt ctcgtgcata tgccattaca ttctccgttt
    61141 cagcggcaaa accgatgcag aacggcgggt tcggcaatga ggcaatagaa gccaaaatat
    61201 cggggttctc atccaattcg atggataacg gtttggcatt tttatctttt ttgaattttt
    61261 gcgtactctt gtttttaacc ttatagtctg agacggcggc aacagaaata aaagcatctt
    61321 gtttttcaat caaacgatgc actgcgcgat gcatatcttc agcgctgacg gcttgaaccg
    61381 tatcggatat gccgaaaggc agcgcggttt gaagctgtcc gtgaatcagg ctgacttccg
    61441 caccggcggc acggcacgcc cgcgccaaag ctacccccat ttttccgctg gaggtatttg
    61501 tgatgcccct gacagggtca atggcttcaa atgtcgcacc tgcggtaatc aagattttct
    61561 tgccccttaa aattttcggt gtccacaagt ccggaagcaa atccagcaat tcggcaggtt
    61621 ccggcatcct tcccatgcca ttttctccgc aagcctgttc gcccaagccc ggcatataga
    61681 cagtaatgcc gtctgaaacc agttgtgcga tattccgttg gttggcaggg ttgagccaca
    61741 tttccacatt catcgcggga gcgatggcaa gcggacattt ccgtgcggct gccagactgg
    61801 tcagtaggtt atctgccacg ccgttacaga tttttgccac ggtattcata cttgccggcg
    61861 caatcagaaa aacatccgca ttccgggtca ggttgatatg ttccataccg tttgaaccgt
    61921 tgccgccgtg cgtgtcggtc aggacaggat tgccgcttaa agcctgaaaa gtcagcggag
    61981 aaacaaattc agttgccgag cggctcataa ccaccgtaac cgaatgcccc tgttttttca
    62041 gcagacgaac caactcgcaa gacttatacg ccgcaatact gcccgttaca cctaaaagaa
    62101 tatgtttgcc cattttgaca atttctaatt acgaagaaaa aacagcagcc attctataac
    62161 aaagtgtgga tacattagat accgtttgga tacaa
//
LOCUS       DAXIHS010000002        62061 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXIHS010000002 DAXIHS010000000
VERSION     DAXIHS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 62061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.002"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(100..747)
                     /gene="adk"
                     /locus_tag="ACFOF2_000062"
     CDS             complement(100..747)
                     /gene="adk"
                     /locus_tag="ACFOF2_000062"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC7106458.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            962..1801
                     /gene="htpX"
                     /locus_tag="ACFOF2_000063"
     CDS             962..1801
                     /gene="htpX"
                     /locus_tag="ACFOF2_000063"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC7106459.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(2102..2662)
                     /locus_tag="ACFOF2_000064"
     CDS             complement(2102..2662)
                     /locus_tag="ACFOF2_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106460.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(2776..3168)
                     /locus_tag="ACFOF2_000065"
     CDS             complement(2776..3168)
                     /locus_tag="ACFOF2_000065"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC7106461.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(3376..3768)
                     /locus_tag="ACFOF2_000066"
     CDS             complement(3376..3768)
                     /locus_tag="ACFOF2_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC7106462.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(3885..4295)
                     /locus_tag="ACFOF2_000067"
     CDS             complement(3885..4295)
                     /locus_tag="ACFOF2_000067"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC7106463.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(4309..4692)
                     /locus_tag="ACFOF2_000068"
     CDS             complement(4309..4692)
                     /locus_tag="ACFOF2_000068"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC7106464.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(5161..6459)
                     /locus_tag="ACFOF2_000069"
     CDS             complement(5161..6459)
                     /locus_tag="ACFOF2_000069"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC7106465.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(6563..7714)
                     /locus_tag="ACFOF2_000070"
     CDS             complement(6563..7714)
                     /locus_tag="ACFOF2_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC7106466.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            8049..9428
                     /gene="norM"
                     /locus_tag="ACFOF2_000071"
     CDS             8049..9428
                     /gene="norM"
                     /locus_tag="ACFOF2_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC7106467.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            9607..10647
                     /gene="murB"
                     /locus_tag="ACFOF2_000072"
     CDS             9607..10647
                     /gene="murB"
                     /locus_tag="ACFOF2_000072"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC7106468.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            10659..11309
                     /locus_tag="ACFOF2_000073"
     CDS             10659..11309
                     /locus_tag="ACFOF2_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC7106469.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            11364..12131
                     /locus_tag="ACFOF2_000074"
     CDS             11364..12131
                     /locus_tag="ACFOF2_000074"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC7106470.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGFPAPDFAPDDSMGKIIRTVCDNS"
     gene            12191..12757
                     /locus_tag="ACFOF2_000075"
     CDS             12191..12757
                     /locus_tag="ACFOF2_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC7106471.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            12777..13667
                     /locus_tag="ACFOF2_000076"
     CDS             12777..13667
                     /locus_tag="ACFOF2_000076"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC7106472.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            13692..14450
                     /locus_tag="ACFOF2_000077"
     CDS             13692..14450
                     /locus_tag="ACFOF2_000077"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC7106473.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWESDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(14505..15170)
                     /locus_tag="ACFOF2_000078"
     CDS             complement(14505..15170)
                     /locus_tag="ACFOF2_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC7106474.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(15370..16143)
                     /gene="folE2"
                     /locus_tag="ACFOF2_000079"
     CDS             complement(15370..16143)
                     /gene="folE2"
                     /locus_tag="ACFOF2_000079"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC7106475.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            16335..17492
                     /locus_tag="ACFOF2_000080"
     CDS             16335..17492
                     /locus_tag="ACFOF2_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC7106476.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(17555..18556)
                     /gene="hemB"
                     /locus_tag="ACFOF2_000081"
     CDS             complement(17555..18556)
                     /gene="hemB"
                     /locus_tag="ACFOF2_000081"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC7106477.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(18739..19023)
                     /gene="yhbY"
                     /locus_tag="ACFOF2_000082"
     CDS             complement(18739..19023)
                     /gene="yhbY"
                     /locus_tag="ACFOF2_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC7106478.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            19133..19753
                     /locus_tag="ACFOF2_000083"
     CDS             19133..19753
                     /locus_tag="ACFOF2_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC7106479.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            19817..21784
                     /gene="ftsH"
                     /locus_tag="ACFOF2_000084"
     CDS             19817..21784
                     /gene="ftsH"
                     /locus_tag="ACFOF2_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC7106480.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            22005..22436
                     /locus_tag="ACFOF2_000085"
     CDS             22005..22436
                     /locus_tag="ACFOF2_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC7106481.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            22429..22707
                     /locus_tag="ACFOF2_000086"
     CDS             22429..22707
                     /locus_tag="ACFOF2_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC7106482.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            22760..23338
                     /gene="pth"
                     /locus_tag="ACFOF2_000087"
     CDS             22760..23338
                     /gene="pth"
                     /locus_tag="ACFOF2_000087"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC7106483.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            23399..23605
                     /locus_tag="ACFOF2_000088"
     CDS             23399..23605
                     /locus_tag="ACFOF2_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC7106484.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            23602..23964
                     /locus_tag="ACFOF2_000089"
     CDS             23602..23964
                     /locus_tag="ACFOF2_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC7106485.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            24195..25610
                     /locus_tag="ACFOF2_000090"
     CDS             24195..25610
                     /locus_tag="ACFOF2_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC7106486.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            25729..26238
                     /locus_tag="ACFOF2_000091"
     CDS             25729..26238
                     /locus_tag="ACFOF2_000091"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC7106487.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKNGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            26405..27787
                     /locus_tag="ACFOF2_000092"
     CDS             26405..27787
                     /locus_tag="ACFOF2_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC7106488.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(27807..28562)
                     /locus_tag="ACFOF2_000093"
     CDS             complement(27807..28562)
                     /locus_tag="ACFOF2_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC7106489.1"
                     /translation="MIKIRNIHKTFGENTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(28572..29288)
                     /locus_tag="ACFOF2_000094"
     CDS             complement(28572..29288)
                     /locus_tag="ACFOF2_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC7106490.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(29278..30105)
                     /locus_tag="ACFOF2_000095"
     CDS             complement(29278..30105)
                     /locus_tag="ACFOF2_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7106491.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            30390..31296
                     /locus_tag="ACFOF2_000096"
                     /pseudo
     CDS             30390..31296
                     /locus_tag="ACFOF2_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            31529..35143
                     /gene="recB"
                     /locus_tag="ACFOF2_000097"
     CDS             31529..35143
                     /gene="recB"
                     /locus_tag="ACFOF2_000097"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC7106492.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPHRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAEISVAAMPSETPTAS
                     DGISIHNFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            35182..35541
                     /locus_tag="ACFOF2_000098"
     CDS             35182..35541
                     /locus_tag="ACFOF2_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC7106493.1"
                     /translation="MNIKHLITTALIASAAFAAQAAPQKPLSAAQTAQHPVVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            35666..36145
                     /locus_tag="ACFOF2_000099"
     CDS             35666..36145
                     /locus_tag="ACFOF2_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC7106494.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTKTDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(36421..37800)
                     /gene="radA"
                     /locus_tag="ACFOF2_000100"
     CDS             complement(36421..37800)
                     /gene="radA"
                     /locus_tag="ACFOF2_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC7106495.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(37978..39042)
                     /gene="hemE"
                     /locus_tag="ACFOF2_000101"
     CDS             complement(37978..39042)
                     /gene="hemE"
                     /locus_tag="ACFOF2_000101"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC7106496.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(39117..40334)
                     /locus_tag="ACFOF2_000102"
     CDS             complement(39117..40334)
                     /locus_tag="ACFOF2_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC7106497.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(40331..41605)
                     /locus_tag="ACFOF2_000103"
     CDS             complement(40331..41605)
                     /locus_tag="ACFOF2_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC7106498.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGNNALATCALVLAALG
                     LGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDG
                     VLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(41680..42441)
                     /locus_tag="ACFOF2_000104"
     CDS             complement(41680..42441)
                     /locus_tag="ACFOF2_000104"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC7106499.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(42462..42881)
                     /locus_tag="ACFOF2_000105"
     CDS             complement(42462..42881)
                     /locus_tag="ACFOF2_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC7106500.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(43132..43452)
                     /locus_tag="ACFOF2_000106"
     CDS             complement(43132..43452)
                     /locus_tag="ACFOF2_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC7106501.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(43475..44101)
                     /gene="upp"
                     /locus_tag="ACFOF2_000107"
     CDS             complement(43475..44101)
                     /gene="upp"
                     /locus_tag="ACFOF2_000107"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC7106502.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(44207..44518)
                     /gene="grxD"
                     /locus_tag="ACFOF2_000108"
     CDS             complement(44207..44518)
                     /gene="grxD"
                     /locus_tag="ACFOF2_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC7106503.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(44619..44833)
                     /locus_tag="ACFOF2_000109"
                     /pseudo
     CDS             complement(44619..44833)
                     /locus_tag="ACFOF2_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            complement(44859..45638)
                     /locus_tag="ACFOF2_000110"
     CDS             complement(44859..45638)
                     /locus_tag="ACFOF2_000110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC7106504.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(45643..45993)
                     /locus_tag="ACFOF2_000111"
     CDS             complement(45643..45993)
                     /locus_tag="ACFOF2_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC7106505.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(45997..46974)
                     /locus_tag="ACFOF2_000112"
     CDS             complement(45997..46974)
                     /locus_tag="ACFOF2_000112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC7106506.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(47008..48138)
                     /locus_tag="ACFOF2_000113"
     CDS             complement(47008..48138)
                     /locus_tag="ACFOF2_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC7106507.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            48306..49007
                     /locus_tag="ACFOF2_000114"
     CDS             48306..49007
                     /locus_tag="ACFOF2_000114"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC7106508.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELVGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            49149..50942
                     /gene="lepA"
                     /locus_tag="ACFOF2_000115"
     CDS             49149..50942
                     /gene="lepA"
                     /locus_tag="ACFOF2_000115"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC7106509.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            51087..52106
                     /gene="lepB"
                     /locus_tag="ACFOF2_000116"
     CDS             51087..52106
                     /gene="lepB"
                     /locus_tag="ACFOF2_000116"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC7106510.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWAVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSDLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(52325..52474)
                     /locus_tag="ACFOF2_000117"
     CDS             complement(52325..52474)
                     /locus_tag="ACFOF2_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106511.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(52520..53425)
                     /locus_tag="ACFOF2_000118"
     CDS             complement(52520..53425)
                     /locus_tag="ACFOF2_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC7106512.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGPYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFNERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            complement(53888..54820)
                     /gene="cysK"
                     /locus_tag="ACFOF2_000119"
     CDS             complement(53888..54820)
                     /gene="cysK"
                     /locus_tag="ACFOF2_000119"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC7106513.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(55014..55619)
                     /locus_tag="ACFOF2_000120"
     CDS             complement(55014..55619)
                     /locus_tag="ACFOF2_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106514.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(55616..56467)
                     /gene="dapF"
                     /locus_tag="ACFOF2_000121"
     CDS             complement(55616..56467)
                     /gene="dapF"
                     /locus_tag="ACFOF2_000121"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC7106515.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFIPEHAPIAAWADRFRGIG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            56605..56901
                     /locus_tag="ACFOF2_000122"
     CDS             56605..56901
                     /locus_tag="ACFOF2_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106516.1"
                     /translation="MFRILLLLFPCAILVACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            56915..57583
                     /locus_tag="ACFOF2_000123"
     CDS             56915..57583
                     /locus_tag="ACFOF2_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC7106517.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(57656..59776)
                     /gene="pnp"
                     /locus_tag="ACFOF2_000124"
     CDS             complement(57656..59776)
                     /gene="pnp"
                     /locus_tag="ACFOF2_000124"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC7106518.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(60013..60876)
                     /locus_tag="ACFOF2_000125"
     CDS             complement(60013..60876)
                     /locus_tag="ACFOF2_000125"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC7106519.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            61075..61950
                     /gene="rfbD"
                     /locus_tag="ACFOF2_000126"
     CDS             61075..61950
                     /gene="rfbD"
                     /locus_tag="ACFOF2_000126"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC7106520.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLLSSGY"
BASE COUNT        14757 a        17667 c        15483 g        14154 t
ORIGIN      
        1 ttcggttgca acacgctgtt tatcaaaaac aggtcgtctg aaaacctgat tttctgtttt
       61 cagagtgcga agcctgcccg tgggtacgac cttttcgatt tatttgccca atgcgcccaa
      121 tacttcggct ttcacggctt ctaccggctg agtgccgtca actttgatgt atttaggcgc
      181 gtgttcgcct tccagtttgc tgtaaaaatc gaccaaaact tcggtttgct cgtggtaaac
      241 ggcaaggcgt tttttcacgg tttcttcttt gtcgtcgtcg cgctgaatca aatcttcgcc
      301 ggttacgtcg tctttgcctt caactttggg cgggttgtag gtaacgtggt aagtacggcc
      361 ggaagccaaa tgcacgcggc ggccgctcat gcggtcgaca atcacgctgt caggcacatc
      421 gatttcaacg actgcatcca aatccacgcc tgcttcaacc atcgcttcgg cttgtgccaa
      481 tgtgcgcggg aaaccgtcaa acaggaaacc gtttttgcag tcgtcttgcg cgatgcgttc
      541 tttgaccatg ccgataatga tgtcgtcgcg caccaagccg ccttcgtcaa tgattttttt
      601 cgcttccaaa cccaagggcg tgcctgcctt aatcgcggca cggagcatgt cgccggtaga
      661 gatttgcgga atgccgaacg ctgcggtgat gaattgcgcc tgagtgcctt tgcccgcgcc
      721 cggtgcgcct aaaagtaatg ctttcatgaa gtgatccttt taaatgtgtt tattggaatc
      781 aaacagatgg tttacgattc tacacggctt ggcacaatag ttcaaaaacc aactgcacaa
      841 actgccattt gcaagcaaat gttttgcata cctttgccga gcctgatatt cacaaggcgg
      901 caactttttg ggacaatccc caaaatttca aaacatcaca ccctaccaaa aaggaacatc
      961 cgtgaaacgc atttttctgt ttttggctac caatatcgct gttttggtcg taatcaacat
     1021 tgttttggcg gttctgggca tcaacagccg gggcggcgcg ggcagcctgt tggcgtattc
     1081 cgccgtcgtc ggcttcagcg gctcgattat ttcgctgctg atgtccaaat tcatcgccaa
     1141 acaatcggtc ggtgcggaag tcatcgacac gccgcgcacc gaagaagaag cctggctttt
     1201 gaacactgtc gaagcccaag cgcggcaatg gaacctgaaa acgcccgaag tcgccatcta
     1261 ccactccccc gaacccaatg cctttgccac gggcgcatcg agaaacagct ccctgatcgc
     1321 cgtcagcacc ggtttgctcg accatatgac gcgtgacgaa gtggaagccg tattggcgca
     1381 cgaaatggca cacgtcggca acggcgatat ggttacgctg acgctgattc aaggcgtggt
     1441 caataccttt gtcgtgttcc tgtcgcgcat tattgccaac ctgattgccc gaaacaacga
     1501 cggcagccag tcccagggaa cttatttcct ggtcagcatg gtattccaaa tcctgttcgg
     1561 cttccttgcc agcttaattg tcatgtggtt cagccgacaa cgcgaatacc gcgccgacgc
     1621 gggcgcggca aaactggtcg gcgcgccgaa aatgatttcc gccctgcaaa ggcttaaagg
     1681 caacccggtc gatttgcccg aagaaatgaa cgcaatgggc atcgccggag atacgcgcga
     1741 ctccctgctc agcacccacc cttcgctgga caaccgaatc gcccgcctca aatcgcttta
     1801 aaccgatttg aaacggcaaa aaccgtacgc gcaagcagta cggttttttg tatatgctgt
     1861 ctgaaaacat caggtttcca taccgacacc aaccgtatcc atttccgcac cggatacgcc
     1921 gcattgatat accctgataa accgtatccg gacagaaaat atagtggatt aacaaaaacc
     1981 agtacggcgt tgcctcgcct tagctcaaag aaaacgattc tctaaggtgc tgaagcacca
     2041 agtgaatcgg ttccgtacta tttatactgt ctgcggcttc gtcgccttgt cctgattttt
     2101 gttaatcccc tatatcagat aaaacagcgg taaaattgcc gtctgaaacc aactctgcgc
     2161 catacgggac acgctctgca cgtctatttg cctccgatat atcctcccta ctgtaggaac
     2221 gtctgccgat ttgcacgaaa tcggcacctc tagcaccctt caggatggcg gacgttatcc
     2281 gttcttggga ttctgcaaga cggtcgacgg cattatacag acgctcggtc tgataccctt
     2341 ccgaagcaac ctgcttcagg tattccaaat aaccgtaacc caacgcccct ccgacaagca
     2401 gggtcgtcaa cgctaccagc gttgccgaac ggattttagg gggcatatct tgaagcatat
     2461 ctctaccgaa ccgaaccaaa gtctctttaa agtcggactc tacacgcgta gaaccctcgg
     2521 aaatcttgaa gttcaactca tactgcttcc gtttgtccgc tgtcaaccgc ttgaggttgt
     2581 catccccgta ttggacggtt gacacaaccc tgtacagact gtcctgtatt tcgaccagcg
     2641 tctgtgcaaa gttggcggtc ataactggat tcccaaccct tgccgccgat tctgatactc
     2701 aaaacggtat ccaggcgttc cagcagggct tcgggagaca gcagtgcgta ttcttcaata
     2761 ttttccatag gttgattata gcagaacctt atacctgtta cagacaaggt tgtcgggatt
     2821 tgaacgtccg tcgagcttgg ttcgtataga gccgtagggg gtgtttaggg ctttggcggc
     2881 agcttgtacg ctggggaacg tgagtgtttc gcctgtggcg gtgtcgagga tggagacagg
     2941 tttttggttt tggggaggcg tattttcctt cattttaagt ttcccgcact ccggacaccc
     3001 atactttgac tttatagctg tgtcgtaata caaaagttgg acaaccccgt gcgtaggaca
     3061 tctaacactg gaaggtttat aaatgccact gaactccaac aattctaaat gtggaaattt
     3121 ctcttgaaag cgtgatttgg cggtatcgaa agtcattcgc ttacccataa tttgtctcca
     3181 taggttatgt gcaaatggat tacacaacca aaatatagaa attttcaaat tttttgagac
     3241 ctacctgaaa tcctgcgcaa gcacaatgta aagaaaaatg gtatacgtct ttactaaatc
     3301 tttacataaa ttaactctaa tcgggatttg aacgtccgtc gagcttggtt cgtaggattt
     3361 agggggttgt gaaccttacc tatcgtaccc aagcactttg tacctgtttg aaataaggtt
     3421 gtcgggcgac gttcgaccct tgagcctgtg attgatttgt tggaaatgta cacctaaagc
     3481 agcacctgcg gcagtaacgc ttgggaacgt gagtgtttcg ttggtcgttg tgtcgaggat
     3541 gaggaggggc ttccctacat ttggaggtgt ttgggttttt gacatcttag cacactcagg
     3601 acacccgtac tttgacttta taagggcatt aaatgtcgaa taacgaacgt tcccgtgcat
     3661 aggacaatta acggttactg ggtaattcac accgtcaaag ccggttagag agatattagg
     3721 gaatatatca gtaagttcag aagctgcttg gttgaaagat ttggccattt gagactccaa
     3781 aattgattgt caaatggatt taatagcaaa tttataaaaa tgtcaaattt ttcgagaagc
     3841 acctgatagt ggcgaggggt atacactgtt aaaaagtgta tacgtcattt agtgcagatg
     3901 tgaaccttat acctccctgc aagtaaagtc tctgtgtgaa cccgaccttt tatacggtca
     3961 gtgatagctg ctgggtttat acccaatgac cttgcagcag atgctcttga ggggaatgta
     4021 agtgtttcgc cagttacagt atcctccaat actgtatttt tagataattt atctattgta
     4081 tcttctggaa ttttgtggga attgacacca taagatgcac attttggaca cccactttta
     4141 gagtccaaca tacttttgaa tgtagagcaa gtaaccctcc cgtgtatagg acagacgatt
     4201 accgtcgggt aacgaacgcc attgaactcc acaagattca aatgtggaaa tttagaggtt
     4261 agcttagaaa ctgcttgagt aaatgtaagt gccatagtgg ttgtgaactt actttaccaa
     4321 cctataacga tacctaatca atcggtcaaa cggagtgcgt ccgcttaaac gtacagctaa
     4381 agtgctgttg gagcaaccga taaatttact ggcagccagt gtacttgcaa actcgtagat
     4441 ttcccctgtc tctgtgtcct ccaacctaat ggcaacggcg tttggagagg gcttttcggg
     4501 atttcgagta cattcaggac atcctgttga ggatttcatg gcagcttgta gattcccgta
     4561 aacaaccgac ccatgcaaag ggcattggat tgttaccggt tcgtagtttt tagtgaactt
     4621 caaaaccgtc ttatcaggaa atttactgga aaaccttaac tgaacatctt gtagcgagag
     4681 tgattttggc attatagacc tttttaaaat gaaagcatta tacaaccaaa atatagaaat
     4741 tttcaaattt tctctgaacg cactggtatc cgatggagct aaatgtaaag aaaaagggta
     4801 tacgtcttta ctaaatattt acacatttta actgtatagt gtttgacaaa caacaggcat
     4861 aggcgcataa tgcacccgta gttagataga tagatagtaa ggagataacc aatgacttag
     4921 cacctgcccc ctctctctcg cgcaccccgc ccctcgcttg cggctaatgg gctttgcgcg
     4981 acatatacac tttcatctcc tgcatggctt aaccgcctat gcaatagtac acacatcaac
     5041 aaatcaggct ggcacgtctt ggcattggct gacgtaccat tgtagcccga aatgttgata
     5101 ccctactatt tatgaccaac ggcggattag ggcttcagac ggcaatttta ctgcaaaacc
     5161 ttatgcgaac ggatgatgca gcacaatcgt ttcttctcgg tcggggccgg tggagacgat
     5221 ggcgaccggc gcgccgcaga cttcttcaat ccgtttcaaa tatgctttgg cgttttcggg
     5281 caatgcgccg tagtccttca cgccgaaagt ggattcacgc cagccgggca tggtttcgta
     5341 aatcggcttg caggcttcta ccgcatcaga gccgcaaggc aggatgtcgg ttttgccgcc
     5401 gtcgggcagt tcgtagccga cgcagatatt gatggtttca acgccgtcca ttacatcgag
     5461 tttggtaatg cacataccgg aaatgccgtt gatttggatg gagcgtttca gggcggcggc
     5521 atcaaaccag ccgcagcggc gcgcacgacc ggttacagaa ccgaattcgt gtccgcgctc
     5581 cgccaaacct gcgcctactt cgtcgaacaa ttcggtcggg aacgggcccg aaccgacgcg
     5641 cgtggtatag gctttgacga tgcccaaaac ataatccagc atttgagggc ctacgcccgc
     5701 gcctgccgaa gccgcgccgg cgaggcagtt ggacgaggta acgaagggat aagtgccgta
     5761 gtcgatgtcc aacaacgcac cttgcgcgcc ttcaaacagc agtttttcgc cgtttttgtt
     5821 tttctcgttc aacacgcggg acacgtcggt aatcatcggc gcaatgcgcg gcgcgacttt
     5881 ttcgataacc gccatcacgt cttccgcttt aaccggttcg gcattgtgca gatgttgcag
     5941 ttggacgttg taataagcaa ggacgacatc cagtttttca cgcagttttt caggatgcag
     6001 caaatcggcg gcgcgaatgg cgcggcgtgc cactttgtct tcgtaggcag ggccgatgcc
     6061 gcggccggtc gtgccgattt tgcctttgcc gcgcgatgct tcgcgggctt ggtcgagcgc
     6121 gatgtgataa ggcaggatca gcgggcaggt cggcgcgatt ttcagacggc cttcgacgtt
     6181 tttcacgcct gccgcgttca actcgtcgat ttcgcccaac agggcttcgg gggagacgac
     6241 aacgcccgaa ccgatgaagc agtccaaact ttcatgcagg atgccgctcg gaatcaggcg
     6301 caaaatggtt tttttgccgc cgacaaccaa ggtatggccc gcattgtggc cgccttggaa
     6361 gcgcaccacg ccgccggctt cttccgccag ccagtcaacg attttacctt taccctcgtc
     6421 gccccactgt gcgccgatta ctacaacatt tttagccata gccatataac ctatcgatat
     6481 taaaaattat tgcggaaatc cccaaaccgg tgcggctttg cgccccgcca cgatttgcct
     6541 ttcatccgcc atgaacgggt atttagcgtt tcacgacctg ccaaacgccg tccgtctttt
     6601 tcagacggcc tgcaagctct tccgaaacat tgtgtccgat accgtagtca atcacgacac
     6661 actgcccttg ttcacgcaag gcttcgactg cttcgcgcgc cgcttcggca tcttccgcat
     6721 cgaccaacac ggcgggctgc cgttcgatgg tgggcaaacg cccgataaag ctgcgcaagt
     6781 cgaaactgaa tcccgttgcc gggcgcgccc tgccgaaata tccgcccaat ccgtcataac
     6841 gcccgccgcg cgcgaccgcg tcgtggaaat cggcggcata ggcggcatac agcaagcccg
     6901 tgtggtaatt gtcgacacgc agctcggaca agtcgatatg gatttcacaa tcggggaatg
     6961 cgtcgcacac cgcctgcaat tcgcccaacg cgccgccgac cgccgacaaa tccggcaacc
     7021 gtccgcgcgc gtcggacaac acttcacgcc cgccgtacag gcgcggcagc agcgagaatg
     7081 cttttgccca cataccgtcc agcttccaag ccttgacctg cgcttcgacc gtctcggtat
     7141 ctttatcctg catcaaggca agcagcgttg cagactgccc cgcatccaaa tgcgccgcat
     7201 cggacaaggc gcgaaatatg ccgatatgcc ccagcgaaag cagcactttg cccatatcgg
     7261 caattttcat gcttttcagc atcaggtcta tcagctcgat gtcgccacgg atgtcggcaa
     7321 aaccgtacat ttccgcccct gcctgcaagg gttcgcgcat attcagcaga ccgtcggact
     7381 gcgcgtgcaa caccggaccg gcataacaca accggttaat cccttggttg gcggataaaa
     7441 gatgggcatc gatacgcgcc acctgcggcg tgatgtcggc gcgtatgccc aactgcctgc
     7501 cgctgagcct gtccgttacc aaaatggttt tcagggaaag ccccgcatcg atatgcgtca
     7561 gcagggaatg tgcgtactcc atcagcggag gctgtaccag ttcataaccg tgtacgcgga
     7621 acagtgccaa caactgctcc ctcgcgcttt caagctgccg cgcgttcgtg ggcagtacgt
     7681 cggcgatatg ttccggaagc tgccatgtct gcatgagaat ctgccttcta ttttatcatg
     7741 taaatacaaa aggctgcccg atccgcaatc agacggcatc tttgccgcaa cgcatcacgc
     7801 cgctgtcaaa acggcgatgc ttccttccga ttcagaatcc gaacagcccc tgtccgacgg
     7861 ctcaatgccg tcaagtcgtt aaaaccaaac tttaccataa aatacacaca atctgaatat
     7921 acttttatac cctgtatccg ccgtctgacg gcacggcatt tttttactat ataatgtgcg
     7981 ccattatttc tcatcacttc ctgctgccgt tttctgcttc agacggcatt tttgttgatt
     8041 gaacacttat gctgctcgac ctcaaccgct tttccttttc cgtcttcctg aaagaagtcc
     8101 gcctgctgac cgctcttgcc ctgcccatgc tgttggcgca ggtcgcgcag gtgggcatcg
     8161 gttttgtcga taccgtgatg gcgggcggtg cgggcaagga agacttggcg gcggtggctt
     8221 tgggcagcag cgcgtttgcc acggtttata ttacctttat gggcattatg gcggcgctga
     8281 acccgatgat tgcccagctt tacggcgcgg gtaaaaccga cgaagtgggc gaaacgggac
     8341 ggcaggggat ttggttcggg ctgtttttgg gcgtgttcgg catggtcttg atgtgggcgg
     8401 cgattacgcc gttccgcaac tggctgacct tgagcgatta tgtggaaggc acaatggcgc
     8461 agtatatgct gttcaccagc ttggcgatgc cggcggcaat ggtacaccgc gcactgcacg
     8521 cctacgcctc cagcctgaac cgcccgcgcc tgattatgtt ggtcagcttt gcggcgtttg
     8581 tgttgaacgt gccgctgaac tatattttcg tttacggcaa attcggtatg cccgctttgg
     8641 gcggcgcagg ctgcggactg gcgacgatgg cggtgttttg gttcagcgcg ctggcattgt
     8701 ggatttatat cgccaaggaa aatttcttcc gcccattcgg actgacggcg aaattcggca
     8761 aaccggattg ggcggtgttc aaacagattt ggaaaatcgg cgcacccatc gggctgtctt
     8821 attttttgga agccagcgcg ttttcgttta tcgtgttttt gattgcgcct ttcggcgagg
     8881 attatgtggc ggcgcagcag gtcggcatca gtttgtcggg gattctctat atgattccgc
     8941 aaagcgtcgg ctcggcgggg acggtgcgca tcggcttttc gcttgggcgg cgcgaatttt
     9001 cgcgggcgcg ttatatttcg ggcgtgtcac tggtgttagg atggatgctc gccgtgatta
     9061 ccgtgctttc cttggtatta ttccgttcgc cgctggtaag tatgtacaac aatgatccgg
     9121 cggttttaag catcgccgcc accgtcttac tgttcgccgg cttgttccaa ccggcagact
     9181 tcacccaatg tatcgcctcc tacgccttgc gcggctacaa agttacaaag gtgccgatgt
     9241 tcatccacgc cgccgccttt tggggctgcg gtctgctgcc gggctacctg ctcgcctacc
     9301 gtttcgatat gggcatttac ggcttctgga cggcattgat tgcctcgctc accatcgccg
     9361 ccatcgcctt ggtgtggtgc ttggaattgt gcagtaggga gatggtcaga tcgcataagg
     9421 ccgtctgaaa actccgccga ttaaaaatat agtggattaa caaaaaccag tacggcgtta
     9481 cctcgccttg ccgtactatt tgtactgtct gcggcttcgt tgccttgtcc tgatttttgt
     9541 taatccacta tagaaacgat gggctgaagc ccgccactgg caatactcat tttaaagttc
     9601 aaacccatgc aacccatccg ataccgaacc gaccttaccc cctacaacac tttcggcctg
     9661 cgcgcccaag cccgagcctt tattgcgctt gaacacgccg acgggttgcg cgacatcgtc
     9721 cggctacccg agttcaaccg agatactgtt ttatggctgg gcggcggcag caatatcctt
     9781 ttgatggagg actacgccgg tctggtcgta catatggaaa acaaaggcat acgcgaaatt
     9841 gctcgttcag acggcatggt tctgattgaa gcgcaggcgg gcgaaatctg gcacgatttt
     9901 gtcctgcacg ccgtcgcgct gggtttgaac ggtttggaaa acctgagcct gattccgggt
     9961 acggtcggcg cgtcgcccgt gcagaacatc ggcgcatacg gcgtagaggc gaaagatgtg
    10021 attcacagcg tgcgctgctt cgatttggat acggaaacct ttgtaaccct ttccaatgcc
    10081 gactgccgct tcgcctaccg cgaaagcctg ttcaagcagg aaggtaaagg gcgttatgtg
    10141 attgtttcgg tcgtatttgc attaaaaacg cattttgtgc cgaacttggg ttacggcgat
    10201 ttggcggccg ccgttgccga actgagcgcg ggcagggtgc cgacggcgaa agatgtttcc
    10261 gatgcagtgt gtgcaatccg caacagtaaa cttcctaatc ctaacgtgct tggtaatgtc
    10321 ggcagtttct ttaaaaatcc cgtcatcggc gcggaaaaag ctgccgccct gttggagcag
    10381 catcccgata tgccgcatta tccgcagccg gacggttcgg tcaaactcgc cgccggctgg
    10441 ctgatcgacc aatgtcgtct gaaaggcttc caaatcggcg gcgcggcggt acacgacaaa
    10501 caggcattgg ttttggtaaa caaaaacaac gcctcggcaa acgatgtccg gcagttggcg
    10561 caacacatca aatttacagt atttgctcgg tttcaggtag aattgcacgc cgaacccaat
    10621 tggctgcctg cttcgttcag cctgtaaatc caaggagtat gcccgtgacc cgccccgcca
    10681 aaatcaatac ttacacacgc atcatcgacg ccagccttgc gcttttcaac gaggaaggcg
    10741 agcgcaacat cagtaccaac catatcgcgg cgcatttggg catcagtccg ggcaatctct
    10801 attaccactt ccgcaacaaa gacgaaatca tcgtccaact gttcaaacgt tacagcgaag
    10861 ccctgcttgc ctacctcaac gaagccgtgt tgccgtctga tgtggaagac tccatcaact
    10921 atatggcggg catttacgac gtgatgtggg aataccgctt cctattcagc gacgtaaaca
    10981 ccctgcttgc acgcagtgcc gaattgttgg gcgaacacaa caccttcacc caagccaaag
    11041 tctccccgct cttggtcaac ctgctgaccc agctcaacgg tctgaacatc atccaagccg
    11101 accaaaccgc tatgaacgat ctcgccgtca atatgtggat ggttacgaaa tactggttcg
    11161 acttcgacag ctccctgcgc ggccgcacca agctgaccga agactccaaa gcacgcggca
    11221 tccgccgcac cttaagcctc cttcgtcctt atcttttgcc cgaacaccgc aagaaatacg
    11281 accggaaaat cggcaacggc aacccgtaaa cccacagcgt ccgaatcagc cttcagacgg
    11341 cacttacccg acggtatcag cgaatgcgcg cagtcccccc gccccatatc tccatcacag
    11401 gcctaggtta cctcggcctg ccgctggcac aaaagtttta ccaacacggc agccgcgtcg
    11461 ccgccataaa acgcagcctg acttcggacg acatcaacct gcccatacat ctcgacacct
    11521 tcgacctcgg cagcaccgat gcctttcaga cggcattgtg gcggcaccac gccgacaaac
    11581 ccgtctggtt cttcctgcta ccgccctcct cgctgacgca ttacgccgac accgtcaaac
    11641 aatgggcaga acttgcccgg gcgtgcaacg tgcaacacct gattttcaca agcagcacca
    11701 gcgtttacgg cgatacagcg cgcgaatgcg acgaaaccgc cacacccgat ccgcaaaccg
    11761 agtccgcccg ccaaatcctc gccgccgaac aacacctgct cgacagcggc atctccaaca
    11821 tcgacatcct gcggctgggc gggctttatt gcgccgaacg ccatcccgtc agccgccttg
    11881 ttcaaaagca aaacatcccg ggcggcaacc gccccatcaa catcgtccac cgtaatatcg
    11941 ccgtcgaaag cctgtttcag acggcattta accccggcgg caggcggctg aaaaacatta
    12001 tcgaaccgcg ccacccgaca cgacgcgaat tctacacgga agaagccgcc aaactcggtt
    12061 tcccggctcc tgatttcgca cccgacgaca gcatgggcaa aatcatccgt accgtttgcg
    12121 ataacagctg aagcctgtaa aataagcgtc aacagcaaca aacaggcatt ctccgccccg
    12181 atcagaaaat atgtccgccc tcctccccat catcaaccgc ctgattctgc aaagcccgga
    12241 cagccgctcg gaacttgccg cctttgcagg caaaacactg accctgaaca ttgccgggct
    12301 gaaactggcg ggacgcatca cggaagacgg tttgctctcg gcgggaaacg gctttgcaga
    12361 caccgaaatt accttccgca acagcgcggt acagaaaatc ctccaaggcg gcgaacccgg
    12421 ggcgggcgac atcgggctcg aaggcgacct catcctcggc atcgcggtac tgtccctgct
    12481 cggcagcctg cgttcccgcg catcggacga attggcacgg attttcggca cgcaggcaga
    12541 catcggcagc cgtgccgccg acatcggaca cggcatcaaa caaatcggca ggaacatcgc
    12601 cgaacaaatc ggcggatttt cccgcgaatc cgagtccgca aacatcggca acgaagccct
    12661 tgccgactgc ctcgacgaaa taagcagact gcgcgacggc gtggaacgcc tcaacgaacg
    12721 cctcgaccgg ctcgaacgcg acatttggat agactaacct tcagacggca tccgacatga
    12781 acagcccttt tcacaacatc ggcatcgtaa cccgccccaa cacgcccgac atccaagaca
    12841 ccgcacacac gctgattacc tttttgaagc agcacggctt taccgtctat ctcgacgaag
    12901 tcggcataaa ggaaggctgc atctataccc aagacaccgt cggctgccat atcgtcaaca
    12961 agaccgaact ggggcaatac tgcgacctgg tcgccgtttt aggcggagac ggcacctttc
    13021 tctccgtcgc ccgcgaaatc gccctgcgcg ccgttccgat tatcggcatc aaccaagggc
    13081 atttgggctt cctgacccaa attccccgcg aatatatgac ggacaagcta ttgcccgttt
    13141 tagaagggaa ataccttgcc gaagagcgca tcctgattga ggccgcactc atccgcgaag
    13201 gcaaaaccgc cgaacgcgcc atcgccctca acgatgccgt cctctcccgt ggcggtgccg
    13261 gacagatgat tgagtttgaa gtcttcgtca atcgggaatt cgtctatacc cagcgttcgg
    13321 acgggctgat tgtctccacc cccaccggat cgaccgccta ttcgcttgcc gccggcggcc
    13381 ccatcatgca ggcaggattg cacgccttca cgctcgtccc catctgcccg caatccatga
    13441 ccaaccgccc catcgccatt ccagacacgt ccgaaatcga aatcctcgtt acccaaggcg
    13501 gcgacgcgcg cgtccatttc gacggtcaaa cccatatcga cgtgcaaaac ctcgaccgca
    13561 tcaccatccg ccgctaccga aaccccttac gcatcctaca tccgaccgac taccaatatt
    13621 tcaaaaccct gcgccaaaaa ctgcactggg gcgaacaatt ggtctgaacc gacccttacc
    13681 ggaaaacacc tatgaatcca aaaactcccc ccgaccatac agaagatgcc gtgcgcctgt
    13741 ccaaacgcat ggcgcaactg gggctttgtt cgcgccgcga agccgacggc tatatcgaac
    13801 agggttgggt aacggtcaac ggcaaaaccg ccgtactcgg tcagaaagtt tcgccggcag
    13861 accgtatcga actaaacaag aaagcccacg aacagcaggc ggcacgcatt accatcctgc
    13921 tgaacaaacc cgtcggctat gtcagcgcac aagcggaaaa aggctataaa tccgccgccg
    13981 aactgattac ctctgaaaac cgctgggaaa gtgacaccag ccgcatccgt ttcgatccga
    14041 aacacaaaat cggtcttgcc cccgccggca ggctcgatat cgattcagtc ggattactgg
    14101 tgttgacgca ggacggccgt atcgccaagc agcttatcgg cgaaaacagc ggcagtgaaa
    14161 aagaatattt ggtgcgcgtg cgcggaaaat tggacgaaaa aggacttgcc ttactgaatc
    14221 acggattgag tttggacggt gagaaactgc gtcccgccca agtagaatgg caaaacgaag
    14281 accaactgcg cttcgtgttg aaacagggca aaaagcggca aatccgccgt atgtgcgaac
    14341 tggtcggact gcgcgtcgtc gggctgaaac gcatccgcat gggtaaggtc aagctcggca
    14401 ggctgccgcc cggcaaatgg cgttatctcg cccccggtga atcgttttaa ataagcatta
    14461 aaaaatgccg tctgaaaatc cttgcgtttt cagacggcat tgccttatgc ccaaataacg
    14521 gtttcttcca agggcctgcg cgccttcgtg gcgatttcct gaacgcggta accgaatgtc
    14581 gcggcgacgg acacgcccca ttctgccgca tcgaacaaac cgaactgccc ggacaatacg
    14641 cgttccatat cggcatagtt gaaaccttcc accgggcagg aatcgatacc tgccatcgcc
    14701 gcacccgtca tcatgttgcc caacgcgata taggtctggc ggcaacacca gtcaaacaag
    14761 gcgcgcgaat cgtccaaaat cttgatgtcg tcggcttgaa acgcctgata cctcgccaaa
    14821 gattttgcca tggcatccgg ttcggtaacg ccgcgccgtt tgaggctttc caacataaac
    14881 gggctgtcga agcgggcatt tttctttgcc aaaaacacca ccaaatgact ggcggtatcc
    14941 aaagcatccg ccatacccca agaaaacggc ttgattgcct gtcggatttc ggggttttga
    15001 accacaacaa actgccaagg ctccgaaccg accgaactgg gcgacaaacg cccgagttct
    15061 aaaataaact gaaaatcctc ggcactgatt ttgcgtgccg catcgtaatg ccggcatgat
    15121 ttacggtttt taaatgcgga tagaacctgc tctctgtcta ataccgtcat agtgcatcct
    15181 tttattactg gtattgcaaa ttgatttcaa accgatacgg cactgccgca ccatacaatc
    15241 cgtcctcgcc atcaagcttc atccggactt tacccatctc tctacggctg ggaaaacaaa
    15301 gcggcggcag actcgggaat aacgccccaa tgcctttcag acggcatttg ggcatcattg
    15361 caaacgcgcc tacgggtagg cgatataggc ataagccgaa tggttgtgga tagactcgaa
    15421 attctcactc tcgacgacga aactcttgat gcgtttgtcg gcgatcagcg aagtagcgac
    15481 atcacgcacc atatcttcca cgaatttcgg gttttcgtag gctttttcgg taacatattt
    15541 ttcatcgggg cgtttgagca aaccgtagag ttggcagctt gcctgcgcct ccacataatc
    15601 aatgacttcc tcgataccga cttctgcatc ggcagtcagg ctgacggtaa catgcgaacg
    15661 ctggttgtgc gcgccatatt gggaaatttc tttggaacac gggcaaagcg aggttacggg
    15721 aatcatgacc ttcatactgt agccgtatgc cccgtctttc atttcgcccg tgagacagac
    15781 atcataatcc agtaaggacc gaataccgga aaccggcgcg gttttcttgc ggaaaaacgg
    15841 aaaagaaacg ctgattttgc cggcgcggga atctaaaagc gcgaccattt cggcagtcag
    15901 cctgtgcaat tgtgcaaaat ccaaggcttc agaatgttgc tccatcaatg cgacaaaacg
    15961 cgacatatgc gtccccttct gctcagcagg caggtaaacc gtcatggtca gacgggcaac
    16021 ggtggactgg atgccttctg cagtttgcag ggtaatcgga aagcgcaggt ctttgatacc
    16081 gacctgattg atcggcagat tgcgcaaatc tcggctggat tgcacgtctg caatagtgtt
    16141 catgagttgt ttttccttaa tattcatgta attgttgaga tgtgtgcatg gtatggcgtt
    16201 tccgccgggg aatataccgt caatctgcaa atgcgagatt atatcactcg cttttagcag
    16261 ctgcattgat tgtttctatt tttcagatag tgataaaatc ttcgattatt cacatctaca
    16321 taggcagaga ccaaatgaaa ttcgccacga aagccattca ttccagctac gattgcgacg
    16381 aacacaaccg cgcgctgatg ccgccgattt atcaaaacag tatgtttacg ttgcacgaga
    16441 ttggcgaaaa tgtgccttac cgttattcgc gcctgagcaa cccgacccgt cagattttgg
    16501 aagacaccgt tgccgatttg gaacacggtg cggcaggctt tgcgttttcc agcggtatgg
    16561 cggggattga tgccgtatgg cgtaccttcc tgcaaccggg cgataccatc gtcgcggtcg
    16621 ccgatattta cggcggcgcg tatgacttat tggtcgatgt ttatcaaaaa tggggggtaa
    16681 atgttgtttt tgccgattta ggcaatcccg acaatttgga cgaattgctc aaagcgcaca
    16741 aggtcaaact ggtttggctg gaaacgccgt ccaatccgct tttacgcttg gtagacatca
    16801 aagcccttgc cgcaaaagcc aaagcgaccg gcgcgctggt cggcatcgac aacacctttg
    16861 ccacgccgta tctgcaacag ccgttggata tgggttgcga ttttgtgttc cattccgcta
    16921 ccaaatattt gtgcggccat tccgacgtat tgatgggcat cgtcgttgcc aaaaccaaag
    16981 agctggctca gcctttgcac gatatgatgg tgcataccgg tgcgattgca ggcccaatgg
    17041 actgctggct ggtgttgcgc ggcatcaaaa cgcttgccct gcgtatggag gcacactgca
    17101 aaaacgcact cgaaatcgcg cgccgtctgg aagaccatcc tgccattgaa aaagtgttcc
    17161 atcccggcct gccgtcgcac gaacattatg aactcgccca agcgcaaatg cctaaaggca
    17221 tcggcggcgt ggtgacggtt tatctcaaaa acgacacgcg cgaagcggca aacagcgtga
    17281 ttaaaaacat gaaactggtc aaaatggctt ccagcctcgg cggcgtggaa agtttggtca
    17341 accattgcta ttcccagtcc cacagcggcg tgccgcataa tgtgaaaatg gaaatgggca
    17401 tcaaagtcgg cctgctgcgt ttctccatcg gcatcgaaga cgcggacgat atttggaacg
    17461 atatttccgc cgcactcgat acgactttgt aaactgtaaa aatgccgtct gaaaccatgg
    17521 tttcagacgg catttcaatt aacccggccg aaaatcaacg cttcaacatc tttgccgcct
    17581 caatagcgta ataggtcaaa atcccgtccg cacccgcacg tttgaatgcc agcaggcttt
    17641 ccaaaaccac tttgccgccg tccagccagc cgttggcaat cgctgcctgc aacatcgcgt
    17701 attctcccga aacctgatag gcataagtcg gtacgccgaa ctcgtccttc acgcggcgga
    17761 caacgtccaa atacggtaaa ccgggcttca ccatcaccat atccgcacct tcccgaatat
    17821 cgagcgccac ttcattcagc gcctcatcgg tatttgccgg atccatctgg taggttttct
    17881 tgtccgcctt gcccaaattg cccgaactgc ccaccgcatc gcggaacggc ccgtaaaacg
    17941 ccgaagcata tttggcggaa tacgccataa tccgcgtgtg gatatgcccg gcatcctcca
    18001 acgcctcgcg aatcgcaccg atacgcccgt ccatcatatc ggaaggggca accacctgtg
    18061 cgcccgcttc agcgtggcac aaagcctgct tgaccaaaac ctctacggtt tcatcgttca
    18121 tcacataacc gttttcgtcc gtcagcccgt cctgaccgtg aaccgtataa ggatcgagcg
    18181 cgacatccgt cataatgccc agttcgggaa acctctcgcg cagggcgcgg acggctgccg
    18241 gcacgagtcc ttcgggattg tacgcttcct ctgcacgctc ggttttgttt gccgtaacca
    18301 cgggaaagag tgccaacatc ggaataccga gctttaccgc ctcttccgcc gtaaacagca
    18361 gcctgtccaa actttgacgc ttcacacccg gcatagaagg cacatcctcc tcgcgcgccg
    18421 acccctccaa tacgaacacc ggataaatca aatcatcggc ggtcagcgtg tgttcgcgca
    18481 tcaggcggcg tgaaaaatcg tccctgcgca tacggcgcat acgcgaagcc ggaacattgc
    18541 ggtaaggaaa ctgcataagc cctccaatca tcctgtcaac aattcaaacc atacggaagc
    18601 cgccgcaact tgcagacagc atcgggcggc aatcaaaatc cgttttcacc ggaaagggtt
    18661 tcggcccgcc gaaatgccgc gccgaaggac ggcgtcccgc ccattaaaga acaatagcaa
    18721 aacaacagct tcaggctgtc agccttcgat attcttacgc cacaatacca aaagtttccc
    18781 gatatgctga acaggttgcg cgtcaaccgc ctcacataag gcagtgcaga tttcgatacg
    18841 ttcggcacgg tcgtcgccga atacgcgcac tttaatcagc tcatgcgccg tcaatgccgc
    18901 atcggtttcc ttgatgaccg cgtccgtcag accctgctga ccgaccatca caacaggatg
    18961 gagatggtgc gcgcgcgctt tcagttccaa aatttctttg gtgttcaatt tggtatccgt
    19021 cattttccta ctgcttgaat aaaaagaata atgcggcatt gtacgcgatt tggcacgaca
    19081 tcggcaaaat taacgtacaa tacgcggttt ccattccgac ccgcgaacca ctatggctgt
    19141 acgttccaaa tcctcaaaag cgtggctgca cgaacacgtc aacgaccact acgtccatat
    19201 ggcgcaaaaa gacggctacc gcgcccgtgc cgcatacaaa cttttggaaa tcaacgacaa
    19261 agacaaatta atcaaacccg gcacggtact tgccgacttg ggcagcgcgc cgggaagctg
    19321 gtcgcaggtt gccgccaagc tgacgggttc ttccggagca gttttcgcct tggacatcct
    19381 gcctatggaa gccatagggg gcgtctcctt cattcagggc gacttccgcg agaacgacgt
    19441 actggcacaa ttcgaaacct tgttggacaa ccgcccgctc gaccttgtaa tttgcgatat
    19501 ggcgcccaat atgtcgggaa acgccgtaag cgatcaggca cgcagctttt atctgtgcga
    19561 actggcttta gacttcgcct cgcaacacct gaaaaccggc ggcagctttt tggtcaaagt
    19621 ctttcagggt gcaggctatc aggaatacat ggcagccatg cgcgaaattt tcggcacggt
    19681 gcagacgcgc aaacccgaag cctcgcgcaa tcgctccagt gagatttatt tattgggcaa
    19741 aaataaacgc tgacaataca gacggcgtgc tttacaatca tttccgtttt acacctcaat
    19801 tatggagcct tgctaagtgg ggaacacctt taaatcaatc cttgtctggg tcgccttggg
    19861 tatcggcctg atggctgcgt tcaacgcttt agacggtaaa aaagaagaca acgggcaaat
    19921 cgaatattct cagttcatcc aacaggtcaa caacggcgaa gtatccggcg tcaacatcga
    19981 aggatccgtc gtcagcggct acctgattaa gggcgagcgc accgacaaaa gcaccttctt
    20041 caccaacgcg cctttggacg acaacctgat taaaacactg ctcgacaaaa acgtccgtgt
    20101 aaaagtaacg ccggaagaaa aaccgagcgc gctggctgcc ctgttttaca gcctgctgcc
    20161 cgtcctgctg ctgattggcg cgtggttcta ctttatgcgt atgcagacgg gcggcggcgg
    20221 caaaggcggc gcattctcat tcggcaaaag ccgcgcccgc ctactggaca aagatgccaa
    20281 caaagtgacc tttgccgatg tcgccggctg cgacgaagcc aaagaagaag tgcaggaaat
    20341 cgtcgattac ctcaaagcgc cgaaccgcta tcaaagcctc ggcggtcgcg tgccgcgcgg
    20401 catcctgctg gcgggcagcc cgggtacggg taagacgctt ttggcaaaag ccattgcagg
    20461 cgaagccggc gtgccgttct tcagcatttc cggttccgac tttgtcgaaa tgttcgtcgg
    20521 tgtcggtgcg agccgcgtcc gcgatatgtt cgagcaggcg aagaaaaacg ctccctgcat
    20581 catctttatt gatgaaatcg acgcagtcgg ccgccaacgc ggcgcaggtt tgggcggcgg
    20641 caatgatgag cgcgagcaaa cattaaacca attgttggtt gaaatggacg gttttgagag
    20701 caatcagact gtaattgtga ttgcggcaac caaccgcccc gacgtactcg atcctgcgct
    20761 gcaacgcccc ggccgtttcg accgccaagt ggttgtcccc ctgccggaca tccgggggcg
    20821 cgaacagatt ttgaacgtcc attctaaaaa agtgcctttg gacaaatctg tggatttatt
    20881 gtccctcgcg cgcggcacgc cgggtttttc cggcgcggat ttggcgaact tggtcaacga
    20941 agccgccctg tttgccggcc gccgcaataa agtcaaagtc gatcagagcg attttgaaga
    21001 cgccaaagac aaaatctata tgggtccgga acgccgcagt atggtgatgc acgaagacga
    21061 aaaacgtgcg acggcgtatc acgaatccgg acacgcgatt gttgccgaaa gcctgccctt
    21121 taccgacccc gtccacaaag taaccattat gccgcgcgga cgtgcgctgg gtctgacttg
    21181 gcagcttccg gagcgcgacc gcatcagtat gtataaagat cagatgttga gccagctctc
    21241 catcctgttc ggcggacgga ttgccgaaga catcttcgtc ggacgcatct ccaccggcgc
    21301 atcaaacgac tttgaacgcg caacccaaat ggcgcgcgaa atggtaacgc gctacggcat
    21361 gagcgacaaa atgggcgtga tggtttatgc ggaaaacgaa ggcgaagtct tcttaggacg
    21421 cagcgtaacc cgttcgcaaa acatttccga gaaaacccag caggacatcg atgcggaaat
    21481 ccgccggatt ttggacgagc agtatcaggt tgcctacaaa atcctcgatg aaaaccgcga
    21541 caagatggaa accatgtgca aagccctgat ggaatgggaa accatcgacc gcgatcaggt
    21601 actggaaatc atggcgggca aacaacccag tccgcccaag gattacagcc acaacctgcg
    21661 cgagaatgcg gacgcggcgg aagataacgc gccgcacgct ccgactcggg aagaaaccga
    21721 agcacctgcc ccggcagaca ccgcttcgac agagtccgag cagcagcctg aaaacaaggc
    21781 ttaacttccc gaacaaacgg cagcccgcaa gctgccgttt tcccatcctg ttttcagacg
    21841 gcattttccg ctcaatgccg tctgaaacgc caaagccgta tcggtttttg cggatctgcg
    21901 atatggcggt ttaaattgaa atcagacgag gcaaggctgt accggtttaa aattaaaccg
    21961 ctataatacc gcctttcgaa tacacgcccg cattggtgat gcctgtgaaa aaagttgaaa
    22021 aaaacatctt ggtgctgcac ggcgcggaca aaatgttcga gctggtcgat aaggttgaag
    22081 actatccgca ctttctgccg tggtacagca agaccgaggt catcgggcgt agcggcaacg
    22141 aactgaaggc gcggctgttt atggactata tgcacgttcg ccaatcattc gccacgcaca
    22201 accgcaacat tccgggcagg gaaatccgta tggaactgct cgagggtccg ttcaaaacct
    22261 tacgtggaac gtggaaattt atcgatttgg gcgacgatat gtgcaaaatc gaattcaatt
    22321 tggaatacga tttttccaat gccgttttgt ccgccttaat ttcccccgtc ttcaaccacc
    22381 tttccgccac gctggtcgaa gcgttcgtca aagaggcaga ccgccgttat gcttgaaatt
    22441 gagattgtgt acgggctgcc cgaccgacag gttttgaaaa ccatgcagct tgccgaggga
    22501 acaaccgtcc gcgccgccgc actgcaaagc ggtttggacg gcatatttga agatttaaac
    22561 ctgcattccg cgcctttggg cattttcggc aaagccgtca aagacgacac gcccctgcgc
    22621 gacggcgacc gcatcgaagt gtaccgcccg ctgttgatcg accccaaaga agcgcgccgc
    22681 aaacgcgttc aaaatcaaga agaataacca tgccgtctga agccttcaga cagcattcga
    22741 cagaaacacg gaaaatatca tgtcaaacaa aatcaaaatg cttgtcggat tgggcaaccc
    22801 cggcaaagaa tacgaacaaa cccgacacaa tgcgggtttt tggttcctcg acgaactggc
    22861 gtggaaatgg aaggcttcat ttaaagaaga aaaaaaattc ttcggcgaag tcgcccgtgc
    22921 cgccctgccc gacggcgacg tttggctgct caaacctgcc acgttcatga accgttccgg
    22981 acaggcagtt gccgcgcttg cacagttcta caaaatcaaa cccgaagaaa tcctcgtcgt
    23041 ccacgacgaa ctcgacattc cctgcggacg gattaaattc aaactcggcg gcggcaacgg
    23101 cggacacaac ggcttgaaag acattcaggc aaaactcggc acggcagact attaccgcct
    23161 gcgcctcggt atcgaccacc cgggcgaccg caacctcgtc gtcggctatg tcctgaacaa
    23221 acccagtacg gaacaccgcc gacagattga cgatgccgtc gccaaatccc tgcaagccat
    23281 acccgacatc cttgccggca aatgggaaga agcaacccgc ttcctgcaca gcaaatgacc
    23341 cgatgccgtc tgaagccctt tcagacggca tgttcccgat ttccatatcc gaacagtcat
    23401 gaccgaactc aagcagctta tccaaaccga atccatcccc gtcatcgaag aaaccctcga
    23461 tttcctgctg tacgaatgca gcatcgacga cgcaccatcc gccgaagaag tggcacaatg
    23521 gcgcgacata cttgccgcac gcggcggcaa attcctgcgc ctgtccaaaa tctgccaaac
    23581 gtggctggac gaggaggcgg catgaagctg ccgcgcaacc gcttcagcct gctttccgca
    23641 ttgtggtttg ccggcggcat ctattcgctg ctcttcaaag ctgccgacac cgcgccgccg
    23701 ccgtttccgc atttcgacaa agcagcacac cttgccctgt ttttcgcaca aatctggctt
    23761 ttgaccaaag cattcaaaac cggaaaactt cccatcccct accgcagcct gatggtcttt
    23821 gccctctgtt tcgccctctt cagcgaatgc gcgcaggcat ggtttaccgc aacgagaacc
    23881 ggcagtttgg gcgatgttct tgccgatatg gcaggtacgg ttctcgcact ctttgccgcc
    23941 cgcgccgccg accgcccgga ctgaatcggt ttatttcccc caaacaggat gcacctcttc
    24001 ccgatacatc ctatttttcc ccttacatat caatcccatc tcataaacaa aaccgacaaa
    24061 tcgtttacaa tatatttaca ctacatcaga ttacaaatat actcgaacca gttgtagaat
    24121 cggcggttcc acaaaccgtt tcggattgca cgacacaaaa gaaaaaccga ttttattgcc
    24181 ttaaaggagt attcatgaac ctgcatgcaa aggacaaaac ccagcatccc gaaaacgtcg
    24241 agctgctcag tgcgcagaag ccgattaccg actttaaggg cctgctgacc accattattt
    24301 ccgccgtcgt ctgtttcggc atttaccaca tcctgcctta cagccccgat gccaataaag
    24361 gtatcgcgct gctgattttc gttgccgcac tttggtttac cgaagccgtc cacattaccg
    24421 taaccgcact gatggtgccg attctcgccg tcgtactcgg tttccccgac atggacatca
    24481 aaaaggcgat ggctgatttt tccaacccga ttatctacat ttttttcgga ggcttcgcgc
    24541 ttgccaccgc cctgcatatg cagcggctgg accgtaaaat cgccgtcagc ctgttgcgcc
    24601 tgtcgcgcgg caatatgaaa gtggcggttt tgatgttgtt cctcgttacc gcctttctgt
    24661 ccatgtggat cagcaacacc gccaccgccg cgatgatgct gcctctagca atgggtatgc
    24721 tgagccacct cgaccaggaa aaagaacaca aaacctacgt cttcctcctg ctcggcatcg
    24781 cctattgcgc cagcatcggc ggcttgggca cgctcgtcgg ctcgccgccc aacctgattg
    24841 ccgccaaagc cctaaatctg gacttcgtcg gctggatgaa gctcggcctg ccgatgatgc
    24901 tgttgattct gcccttgatg ctgctctccc tgtacgtcat cctcaaacct aatttgaacg
    24961 aacgcgtgga aatcaaagcc gaatccatcc cttggacgct gcaccgcgtg atcgcgctgt
    25021 tgattttcct tgccacagcc gccgcgtgga tattcagctc caaaatcaaa accgccttcg
    25081 gcatttccaa tcccgacacc gttatcgccc tgagtgccgc cgtcgccgtc gtcgtcttcg
    25141 gcgtggcgca atggaaggaa gtcgcccgca ataccgactg gggcgtgttg atgctcttcg
    25201 gcggcggcat cagcctgagc acgctgttga aaacatccgg cgcgtccgaa gccttgggac
    25261 agcaggttgc cgccaccttt tccggcgcgc ccgcattttt ggtgatactc atcgtcgccg
    25321 ccttcattat ttttctgacc gagttcacca gcaacaccgc ctccgccgca ttgcttgtac
    25381 cgattttctc cggcatcgct atgcagatgg ggctgcccga acaagtcttg gtattcgtca
    25441 tcggcatcgg cgcatcttgt gccttcatgc tgccggttgc cacaccgcct aacgcgattg
    25501 tgttcggcac gggcttaatc aagcaacgcg aaatgatgaa tgtcggcata ctgctgaaca
    25561 tcctctgcgt agtattggtt gctctgtggg cttatgctgt actgatgtaa accatcgacc
    25621 taaacaacaa gaccgtctga aagaatattt ttcagacgac cttgaagttt gtcgttcaga
    25681 cacaatttgt cgaatcattc aaaaccggat tctaacgaaa ggaaacccat gattatcctg
    25741 cacaccaaca aaggcgacat caaaatcgaa ctcgatttcg acaaagcccc cgttaccgcc
    25801 aaaaacttcg agcaatacgt caaaaacggc ttctacgacg gcgtaatctt ccaccgcgtc
    25861 atcaagggct tcatgattca aggcggcggc atggatgaaa acatgaacga aaaagaaacc
    25921 cgcgatccga ttcaaaacga agcgtccaac ggcctgccca acgataaata caccatcgcc
    25981 atggcgcgta cttccgatcc ccattccgcc agcgcgcaat tcttcatcaa cactgccgac
    26041 aacgctttcc tgaacttccg ttctaaagag ctgtacggca aaaccgtcgt ccaagactgg
    26101 ggctatgccg tattcggtaa agtcgttgac ggttttgacg ttgtcgatgc catcgaaggc
    26161 gtttctacca aacgtcatgg ttaccacgac gacgtaccga gcgaacctgt cgtcatcatt
    26221 aaagccgaag cggtataaac cgacaatccg aaagcagcct gcacaaaggc tgcttttatt
    26281 tcagacggca tgttatttga tgtaaccgtg ctatgccgtc tgaaaaccgg acgcaagcct
    26341 tcagacggca taccgctatg ttaaaatatg cccgtttttc ctaaacggac acaaaggaaa
    26401 ccttatggca agcatcgccc gcgacatctt caaagcctac gacatccggg gtattgtcgg
    26461 caaaaccctg accgacgaag ccgcctacct tatcggcaaa gccatcgccg ccaaagccgc
    26521 cgaaaaaggc attacccgca tcgcgctcgg acgcgacgga cgcttgagcg gtcccgaact
    26581 gatggaacac atccggcgcg gctttaccga cagcggcatc aatgtcctca atgtcggtat
    26641 ggttgccact cctatgctct acttcgccgc cgtcaacgaa tgcggcggca gcggcgtgat
    26701 gattaccggc agccacaatc cgcccgatta caacggcttt aaaatgatgc tcggcggcga
    26761 cacgcttgcc ggcgaagcca tccaagaact tttgtccatc attgaaaaag acggttttgc
    26821 tgccgccggc aaacaaggca gcgtcaccga aaaagacatc tccggcgaat acctcaaaca
    26881 cattaccgga cacatcaggc tcaaacgccc gatgaacatc gccattgacg cgggcaacgg
    26941 cgtgggcggc gcgtttgccg gcaaactcta caaaggcttg ggcaacgaag taaccgagct
    27001 tttctgcgac gtggacggca ctttccccaa ccaccatccc gacccatcca aaccgaaaaa
    27061 cctgcaagat ttgattgccg cgctgaaaaa cggtgatgcc gaaatcggct tggcgtttga
    27121 cggcgatgcc gaccgcttgg gtgtggtcac caaagacggc aacattattt atcccgaccg
    27181 ccaactgatg cttttcgcgc aagacgtgtt gagccgcaat cctaaagcca aagtgatttt
    27241 cgacgtcaaa tccacccgtc tgcttgcgcc ttggattaaa gaacacggcg gcgaacctgt
    27301 gatggaaaaa accggccaca gctttatcaa atccgccatg aaaaaaaccg gcgcgccggt
    27361 tgccggcgaa atgagcggac acatcttctt caaagaacgc tggttcggct tcgacgacgg
    27421 tctgtacgcc ggcgcacgcc tcttggaaat cctgtctgcc tccgataatc cgtccgaagt
    27481 gttaaacaac ctgccgcaaa gcatttccac gcccgaactc aacatcgccc tgcccgaagg
    27541 cagcaacggc catcaggtta tcgacgaact cgccgccaaa gccgaatttg aaggcgcaac
    27601 cgaaatcatc accatcgacg gtctgcgcgt tgaatttccc gacggcttcg gtctgatgcg
    27661 tgcttccaat accacgccga ttctggtgtt gcgttttgag gcggatacgc aagaagccat
    27721 cgagcggatt caaaaccaat tcaaagccgt catcgaaagc aatccgaatc taatctggcc
    27781 tctgtaaaca caggaaaaat gcttaatcaa atcttggtag attggatttg gcttaaaaat
    27841 ctccgcgtcc gttcgtgttt ggggtggtcg aacaactctt tcgggctgcc ctgctctacg
    27901 ataacgccgc cgtccatcac gacaacggta gtcgcaactt ccagcgcgaa cttgatttcg
    27961 tgggtaacga cgaccatcgt ccaaccttcc cgcgccaatt ccttcatggc gttcaacacg
    28021 tcttgcacca actcggggtc aagcgcggaa gtcggttcgt caaacagcat cagctcgggc
    28081 tgaatcgcca atgctcgggc aatgccgacg cgctgctgct gaccgccgga aagctggtag
    28141 ggatacaaat ccactttgtc gcccaagccg actttttcca gcagtttcag agcctcttcg
    28201 cgcgcttggg cggcaggctt gccctgtacg gcaaccggtc cttccatcac gttttccaag
    28261 gcggttttgt gcggaaagag gttgtattgt tgaaacacca tgcctgattt gcggcgcagt
    28321 gccaaaatat cgtgtttgct tggtttttta gaaaaatcga ttttcagcgg tcgctcgttg
    28381 tcgaactcga tttgtccgtc ttcgggcatt tccaacgcgt ttaggcatcg cagaaacgtc
    28441 gttttgcctg agccggaagg cccgaggatg acgaccacct gccctttgca cacatccaaa
    28501 ttgatgccgc gcaaaatggt attttcgccg aaggtcttat ggatattgcg gattttaatc
    28561 atgacaactc cttatttggc gacatagcgg tcgaagcgtt tttccaagcg cgcctgaatc
    28621 aggaacagca ctttgcaaaa acaccagtaa accaaagcgg cttcgatata gacgggcaaa
    28681 aagtcataag tgcggtttgc cgtttcctgc gcgacgcgga ataattccgt taccgtcacg
    28741 actgccgcga gcgaggtgtt tttaaacaaa ccgataaact cgttgctcaa aggcggcacg
    28801 gcaacgcgaa atgcctgcgg cgcgacgatg cggcggaacg tctgcatata ggtcatgccg
    28861 atggagaaac ctgcttccca ttggcctttc ggtacggaca aaattgccgc gcgtatggtt
    28921 tcggaagcat atgcgccgac attgagcgaa aagccgatga tggcggcagg aatcgggtcg
    28981 atatagatgc cgacggaagg cagcccgtaa aacacaatca caagctgaac caacagcggc
    29041 gtaccgcgaa tgacggaaat ataaaattcc accaatttca gcaggatttt ccgcacgatg
    29101 ccgccggcgg gcataatccg caccaaagcc acggctaccg caatcatcat accgataacg
    29161 aaagaagctg ccgccaaagg cagagagacc gcgaagccgg ctttgaccat aggcaaaaac
    29221 gcgctgacaa tcatatcggc gcgtgtttcc gtcataaacg gcagcgaagc aaggaaatta
    29281 ttgaacactg atgtcttttc cgaagaattg ttcgcccagt tttttcagcg taccgtcggc
    29341 tttcagctcg ttgattgccg tactgaattt cgccacggct tcgtcattgc ccttgttgac
    29401 aatcaggccg gaaccgactt tttcatcggc aggtgcggac caaacgattt tcacgcccgc
    29461 attcgggttt ttcttcagat agtccaaaac cgccaattcg tcgttcaggg ttgcatcggc
    29521 acgtttttgt tcaatcaggg tcagcgattg cgccaaaccg tccactgcca ccaaatctgc
    29581 gcctgcagct ttggcttttt cgccgtagtt gctggtcagg gattgtgcgg ttttcacgcc
    29641 tttgatgtcg tcgatagatt tgatgttgct gtcgttacgg gcaaccaata cggcaccgct
    29701 ccagctgtaa ggatcggatt tgtcgaatgt cgcttggcgt tcggggctgg tcagaccgac
    29761 ttggtttgcc accacgtcga aacgccccgc cttcaaaccc gccatcatcg aatcccattg
    29821 cgtttcttta aactcgactt tcacgcccag tttttccgcc acggcgcggg ttacttccac
    29881 atcgtaaccg gtcagtttgc cgtccttgtc gtggtaggta aacggtgcgt aagtgccttc
    29941 cgtgccgacg gtaaccgtgc ctttattgtt gatgcgctcg attaaagaac cggaaactgc
    30001 cgattgtgca ggcgcggaag atgctccgct gccgctttcc gaaccgccgc aggccgccaa
    30061 aaccaatgcg gcaatgccgc cgagtacgaa ttttttcaac atcattctct cctgtaaaaa
    30121 gtgtgcaagt gcggcattct aagtgaaatt gcggcaaaca caaagaatac tttttttata
    30181 tggttattcc aaatcggaat ggaaaagata tgccgaagcc gccggaagcg gaaataaagg
    30241 gggcgggaaa ggcgacgggc ggcacgaaac cgaaaagcgg catcaggtat gcaaacggcg
    30301 gattcacgca cgccgttccg gcaaaaatcc gcctgccgat tcggtgctat aatccatccg
    30361 aaacagatga caccaaaaag gaacatctca tgaacacgcc ccaatccgcc atcattcccg
    30421 accacgccca agccggcatc tttatcgaag ccgacttcgc cgccaaccgc ctcaacgata
    30481 ttaaagccgc ctgccgcgct tcgctcgacg cgttatccgc cttgaaaacc cgctttccgg
    30541 acgatatttt gggtctgacc atcgccttcg gcagcaaagc ctgggcaaca ttcggacata
    30601 cggacgaagg cagtgaaatc aaacccttct ccgaaatggg caacgggctt gcgccgtcca
    30661 cgcagcacga tatgtcccca catccaatct ttccgccaaa acgccgccta cgcgcttgcc
    30721 caatccgttt tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga acacggtttg
    30781 cgcctgtatc aggatcgcgg gctggacggt ttcgtcgacg gcacggaaaa cccgcagggc
    30841 gatgaaaatg tccgcgaagt cgccatcatc cccgaaggaa aacccgatgc gggcggcagc
    30901 tatgtcctgc tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt ccccgtcgcc
    30961 gaacaggagg cctcggtcgg acgcggcaag gaaaccgacg acgaattcgg ccgcgatgtc
    31021 cgcctgcccg attcgcacct cggccgcgtc aacctgaaag aaaacggagt cggcctgaaa
    31081 atcgtccgcc gcagcctgcc cttcggcaaa atcagcggcg aacacggttt gatgtttacc
    31141 gcatactgcc gcacgctgca caatatcgaa gcgcaattat tgagtatgtt cggcgatacg
    31201 gacggcaaaa ccgacctgct gctccaacat ctctccgccg ccgtttcggg cggatactac
    31261 tacgcgccgt ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg tctgaaacca
    31321 tttttcagac ggcattttcc cgttttatag tggattaaca aaaaccagta cggcgttgcc
    31381 tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc
    31441 gtactatttg cactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    31501 ccgtccaacc gttttccgag cgcgcactat gtctgcaccc atccaagcat tcgactcgct
    31561 aaccgtcccc attgccggca ccaacctgat tgaggcctct gccggcaccg gcaaaaccta
    31621 cggcattgcc gccctgttta cgcgcctgat cgtattagaa caaaaaaacg tcgagcgcgt
    31681 attggtcgtt accttcacca aagccgccac cgccgagttg aaaacacgcc tgcgcgcacg
    31741 tttggacgat gtgttgcagg ttttagaaag caaagaaatt gccgaacttg gagacggcgc
    31801 gctttcagac ggcatcgccg cctattgtgc ggaacaccac aaaggcgaca ccttcctgcc
    31861 cgcactctta aaacaggctt tgcaaaaaga gggccggacg cgtctgattg tccgcctcaa
    31921 ggccgccatc gggcaattcg acaacgccgc catctacacc atccacggct tctgccagcg
    31981 catcctgcgc gactacgcct tcttgtgcca agcaccgttc gatgtcgaac tgaccgaaga
    32041 agacggcgac cgcctgctta tcccggcgca agatttttgg cgggaacgcg tcagcaatga
    32101 tccggtgctt gccgcattgg cgtttaaacg caaagctgtg ccgcaaaccg tccttgccca
    32161 aatccgcgcc tacctgtccc gcccgtacct gaatttccgc cgtccgcagg cggatttgaa
    32221 acaggcgcag cgcaacgccg aaacctcatg gcaaaccgtc tgccgcctgc tgcccgagct
    32281 ggaagccggc ttttggcgca tccatcccga cctcaacggc aacagttacc gcaaaaacag
    32341 cttcggcaac ctttttaaag aactggcaca aaaatccgcc gccggacaac tgccctttct
    32401 ggacaaagac acacacgaca gactcttgaa acttgcatcc gacaaactcg aagccggact
    32461 gaaaaaaggc aaaacgcccg atgcggcagt atttgccgaa ttgcagaaac tggcagactt
    32521 cgggcgcgat ttgaacgcac tcgaagaagc ggaagaagca acaatgatcc ggctgcaact
    32581 ggatttaatc gaatacctca accgcagcct tgccgagatg aaaaaatcgc gccgcgaacg
    32641 cggtttcgac gacctgctgc tcgatgtcca caccgcgctg accgacaatc cgcacgccga
    32701 aaccctcgcc cgcgccgttg ccgaaaactg ggaaaccgcg ctgatcgacg agttccaaga
    32761 caccgacccg ctgcaatacg aaatcttcca aaaaatcttc atcgcccaaa accgaccgct
    32821 gttcctcgtc ggcgatccca aacaggcgat ttacagcttt cgcggagcgg acatttacgc
    32881 ctaccttcag gcggcggaag acgcgcggca ccgctacacg ctcgccacca actaccgcag
    32941 ccacgccgcg cttatcggca gcataggcgc gctgttccgc ctcaaagaac gcccgttcgt
    33001 tttggaaaac atcggctatt cggaagtcgg tgcggcgcgt gccgaaagca gattgtcgcc
    33061 caaacgtcct gccgtacagg ttcgttggct gcacgaaaac gacaatgaaa aagccaacaa
    33121 agacgttttg cgccgccgtg ccgccgacta ttgcgccgac gaaatcgccc acgcgctcaa
    33181 cgaagccgcc gaatgccgtc tgaatttcaa aggctgcccg ttgcagtcgg gcgatattgc
    33241 cgtgctggta cgcacgcaca acgaggcggt gatggtttcc gccgccctga aaaaacggca
    33301 ggtgcaaagc gtcctgcttt cgcgcgaatc cgtgttcgcc tcgcccgaag ccgccgccct
    33361 gtccgcactc atcggcttct ggctcgaacc gcaccgcgcc ggaacgctgc gctttgtcct
    33421 aacaagcggc atattcggtt atgacgcgca gcaattgcac gacttcaacc aaaacgaaag
    33481 cgagattttg cattgggcgg aatctgcccg aaccgcgctc gacaactgga atcaatacgg
    33541 cattttcgcc gccatgcagc aattttccca aacacacggc atcgaaacac gcctcttaag
    33601 ccggaacaac gggcgcagcc tgaccaacta tttccaactg ctcgaactgc ttgccgccga
    33661 agacgcgcaa aaccgcaacc ccgccgcgct gcacaaatgg ctgcgcgacc aaatcagcct
    33721 ggccggcaac aacggcggcg acaaccgcgc catccgtctg gaaagcgacg aagatttggt
    33781 caaaatcgtt accatgcacg cctcgaaagg tttgcagtat ccgctggtgt actgcccgtt
    33841 tgcgtgggac gcgcaagata ccgggccgtc cgactggcaa atcctccacc aaagcgcaaa
    33901 ccgaaccgaa ctgttggcaa aggcgcaact gtccgaagac gaacagaaac aatacgccga
    33961 tgaagaaatg gcggaacgcc tgcgcctgct ttatgtcgcg ctgacgcgtg ccgaggaaca
    34021 gctcaacatc tacgccgcgt actccaccaa tactgccgac aaccccctcg cctacctgat
    34081 tgaaggctcg ccccaagaca gccgtgaaac cgtccgccgt gcctatgcgt gtgaaaaaga
    34141 cggcatcgcg atgctcaaac gcaactggcg gcgcgtggcg gacaacgccc cttccggcac
    34201 aaatttcgcc ttcacagaag atgcgccgcc ccctgccgcc tatcgcggca acgccggtca
    34261 aaccgccgaa tttgccgcaa acagcattcc cgaacgcaga ttccgatttg tccgccacac
    34321 cagctttacc gccttaagcc gccataccca aacgcccgac ggcggcgaag aagatgcctg
    34381 cccgtccttg gatgccgccg aaatctcggt agccgcgatg ccgtctgaaa cgccgacggc
    34441 ttcagatggc atatcgatac acaactttcc gaaaggcacg caggcggggc tgtgcctgca
    34501 cgaaattctt gaagatttca aattcggaca agcagccgcc ggacaggaaa ccctcatcac
    34561 cgacaagctg aaaaaatacg gatttgaaga aatatggctg cccgccgttg ccgaaatggc
    34621 ggaagcctgc cgcaaaacgc cgctgacggg ggcatacggc ctgtccgaca tcccgcccga
    34681 gtgccgctgt cccgaaatgg gctttaccct ccacaccgaa gacttcggcc tcaaacggct
    34741 acgcgattgg tttgcccgcg acgacatcag gctgcccgaa gtctgccgtg ccgctgccga
    34801 aacgctagac ttccacaccg tcaacggctt tttaaacggc tttatcgaca tggtctgcca
    34861 agaccccgag ggaaatatct gcgtcatcga ctacaaatca aaccatctcg gcgcggacgc
    34921 atccgcctac acgcggcagg cgatggacga agccgtcgcg caccaccact attaccttca
    34981 ggcgctgatt tacgccgttg ccgccgcacg ctacttcaaa ctgcgcggac aaccgcccgc
    35041 cgccgtttcc gtccgctacc tgtttttgcg cggattggat ggcaaaggcg gcggcgtttg
    35101 gcgttgggac atagatgccg ccgctttgga acagataaaa taacttcccg tcctttcgga
    35161 caacaaccgc aggagaaccc tatgaatatc aaacacctga ttaccaccgc actcattgcc
    35221 tcagccgcct ttgccgcgca ggcagccccg caaaaacccc tatccgccgc ccaaaccgcg
    35281 caacatccgg tcgtttggat cgatgtccgt tccgaacagg aatttagcga agggcatttg
    35341 cacaacgcgg tcaacatccc cgtcgaccaa atcgtccgcc gcatacacga agccgcgccc
    35401 gacaaagaca cgccggtcaa cctctactgc cgcagcggac ggcgtgccga agccgccctt
    35461 caagaactga aaaaagcagg ttatacaaat gttgccaatc acggcggtta tgaagacctg
    35521 ctcaaaaaag ggatgaaatg acgcaatgcc gtctgaaagg acaggctttc agacggcata
    35581 cggcgttccg gcacgcggcg caacatcggg cgcggcgcgc gccgccccgt ccgaagcctg
    35641 ataaacaaac cgaaaaggaa atacgatgaa acacatactc cccctgattg ccgcatccgc
    35701 actctgcatt tcaaccgctt cggcacatcc tgccagcgaa ccgcaaaccc aaaacgaaac
    35761 cgctatgacc acgcataccc tcacctcaaa atacagtttt gacgaaaccg tcagccgcct
    35821 tgaaaccgcc ataaaaagca aagggatgga catttttgcc gtcatcgacc atcaggaagc
    35881 cgcccgccga aacggcttaa cgatgcagcc ggcaaaagtc atcgtcttcg gcacgcccaa
    35941 agccggcacg ccgctgatgg tcaaagaccc cgccttcgcc ctgcaactgc ccctacgcgt
    36001 cctcgttacc aaaacggacg gcaaagtacg cgccgcctat accgatacgc gcgccctcat
    36061 cgccggcagc cgcatcggtt ttgacgaagt ggcaaacact ttggcaaacg ccgaaaaact
    36121 gatacaaaaa accgtaggcg aataagcccg tcgccgacat atggcggatg gaaatcaaat
    36181 gcacacgccc ctcattccca cgaagtcggg aatccaatcg gttgggttac cgtgattttc
    36241 aatcattgca gaaacggacg actccagatt cccacccttg cggggatgac gggcgtgtgt
    36301 gtttttgcca catcacaggc aactttgccg caaatcttat ctgtcgccgg tatgccttct
    36361 tgtttcaatc cgccacaaac caaatgccgt ctgaaacccg atttcagacg gcatttccgt
    36421 ttattccctg ctgtcgcggc aaatatcaat ggcttcctgc aaactcgaaa cgccgtagat
    36481 tttcaggttc ggaaactctt tggcattgcg cggcatattg gctttgggaa cgatggcgcg
    36541 tttaaagccg agcttttccg cttccttgag ccgctcttgt ccgcgcgcga cggggcggac
    36601 ttcaccgctc aaaccgattt ctccaaatgc aaccattttt tcgggcagcg ggcggttgcg
    36661 gaagctggaa agcatcgcga ggatgaccgc caaatccgcc gccggttcgc cgattttcac
    36721 accgccgacg gcgtttaaaa acacatcctg atcgaaacag gcgatgccgc cgtggcggtt
    36781 taatacggca agcagcatcg caaggcggtt ttgttccagt ccgacggtga ggcgtttggg
    36841 ggtgaagccg tgtgcgtcat cgaccaatgc ctgaatttcg accaaaagcg ggcggctgcc
    36901 ttcctgtgta accaaaacgc acgaaccggg cgtgtcgtcg cggtagctgg caaggaagat
    36961 ggcggacggg ttggacacgc ctttcaaacc gttttcggtc atagcgaaca cgcccagttc
    37021 gtttgccgcg ccgaagcggt ttttgatggc gcgtatcatg cggtagttgg aatgttggtc
    37081 gccctcgaaa tacagcacgg tatcaaccat gtgttccagc acgcgcgggc cggcaatcgc
    37141 gccgtctttg gtcacgtgtc cgaccagtat catggcgatg cccatctgtt tcgccatgcg
    37201 cgtcagttgg gcggcacact cgcgcacctg cgacacagaa ccgggggcgg aagtgatttg
    37261 gtcggaatac atggtttgga tggaatcgat aaccacgact tcgggctgat gctgtttcaa
    37321 cgctgcttga atcgcttcca tgcggatttc ggcaagcagg ttcacgcctt cggtgggcag
    37381 ttccaaacgc tgcgcgcgca gggcgacttg ttgggcggat tcttcgccgg aaacgtatag
    37441 cactttacgg ctttgcgcca ttttggcgat ggtttgcaac agcagcgtgg atttgccgat
    37501 gccggggtcg ccgccgagca ggatgaccgc accatcgacc aaaccgccgc ccaatacgcg
    37561 gtcgagttcg cccataccgg tcggattgcg cggcacttcg gtggcggtaa cggcggagag
    37621 ggattggacg gtcgaggtat ccgccgccca agattggaag cgggcgtttt tcggctcggg
    37681 cgcggccaag ctttcctgaa gtgtgttcca ctcgccgcaa tgcgggcatt tgccttgcca
    37741 tttcggggag gtgccgccgc attcggtgca ttggtaaagg gttttaagcg tttttgccat
    37801 cggttttccc tgcatctgaa aacaaaaatg ccgtctgaaa atagggacgg ttgggattgc
    37861 gccaatgcgg caacaccgcc gccgtcatgt actatccgta cacggcggat attgccgtat
    37921 tacctaattt tccatccctc ggctttcaga cggcatatcg ggcggtttcc tgccggctta
    37981 cccgccgtga tactgccgag acagctcgtg tacggtatcg actaagattt tggcgtgttc
    38041 ggggtcggcg tgttggttga tgccgtgtcc gaggttgaag acatggccgc tgccgtgtcc
    38101 gtagtcggct aggatacgtg cgacctcggt gcggatggat tccggcgtac cgaagagggc
    38161 gaacgggtcg aagttgcctt gcagggcgac ttgcttgccg acgcggcggc gtgcttcgcc
    38221 gatgttgcac gtccagtcca agcccaatgc gtctgcgccg atttctgcca tactttccag
    38281 ccacagcccg ccgcctttgg caaatacgat aacaggcacg cggcggcctt cgctttcgcg
    38341 tttgagtccg gcgacgatct ggcggatgta tttgaggctg aattctttaa acgccgcatc
    38401 gctcaacacg ccgccccaag tgtcgaaaat ctgcaccgcc tgcgcgcccg cgtcgatttg
    38461 ggcgttgagg taggcggtaa cggcttgggc gttggtatcg aggattttgt gcagcaaatc
    38521 ggggcgcgag tacatcatgg ttttgatggt gcggaattct ttgctgccgc cgccttcgac
    38581 catataacag gcgagcgtga acgggctgcc ggagaagccg ataagcggta cgcggccgtc
    38641 cagtgcttta cggatggagc tgacggcatc aaagacgtat tgcagttttt ccatatcggg
    38701 aacgtgcagc ttggcgatat cggcttcgtg ttgcagggcg cgtttgaatt tcgggccttc
    38761 gccttcggca aagtacagtc ccaagcccat tgcgtctggg acggtcagga tgtcggagaa
    38821 caaaatcgcc gcgtccaaat cgaagcgttc caaaggttgg atggtaactt cggtcgccaa
    38881 tccggtgttt ttgcacaaat cgaggaagct gcccgctttc gcgcgtgtgg ctttgtattc
    38941 gggcagataa cgccccgcct ggcgcatcat ccaaatcggc gtgtattcga cgggctgttt
    39001 aagcagggcg cggaggaagg tatcgttttt taaaagagtc atacgggttt tctgtcagtc
    39061 tgtttgtcaa gtgaaatggt tttcagacgg catttcaacg atgccgtctg aaggtttcaa
    39121 tgggtttcgg cggaaggtcg gttttcctcg gcaacgcttg ccaaaaccaa gttgcgctgc
    39181 gcctccgcct tctgcggttc tccggtttcg tcaaaaacct ttgccagaac caaacgcgcg
    39241 gaaatactcg gctttaatgc aatgctcgct tcaaggtagc cttttgcctt gccccaaagt
    39301 ttgcggccgt aggcgagccg accgagatac atcagcagaa gcgcgttatc gggctgttct
    39361 ttcagccaag catcggcaaa atcgatggct ttctgctgtt cgcgttcgcc caaaaagcgc
    39421 acgctttcga caaaggcttc caaaagctcg gggcggcggt tgtgcggata atgctgtttg
    39481 acccatttga ccgcatcggc atacagtccc aaacgttcgt acttttccgc aaccgatacg
    39541 ctcaattccc cgtttttgag gctgtcggga atccgcttca ggcaggtttt caaagcggcg
    39601 gcatcggcag catccgccag ctggcggcgg tatgcccaat tttgataccg ttccatttcc
    39661 gatttgccca acgcgcccgc cttggaaagt ttttcggttt ttgccagaac ctgcaacgcg
    39721 tcgcccctgt cgaaagcgta acgaagttgc agacgcacga ggcgcgtaag gttggcattc
    39781 atcttcgccg ccgcatgaag attggcttcc gccgcttcgt aatcgcgccg gttcaacgcc
    39841 gattccgcca acaaaagata acgggaaagc tgctgctttt ccggcagttt ggcgatttcc
    39901 gcaagataac ggtcgcgcag ctcgatgttt tccatctgcc cggcggcatg tgcgcccaac
    39961 atcaatgcca aagtccggtt gtctccggcc tctttgttga ccaacacgcg tgaggcttct
    40021 agttccgcct tttcaaaacg cccttcaaaa tacgccaaac ccgccttgtt caaggcaagc
    40081 gcggccttgc ggcctttacg cgccgaaccg aaacgctgca tcttttcggg gatattgaat
    40141 acgccgataa tgaatttaaa caggaaatac cacaccacga cggcaatcag cgaacctaac
    40201 acaaaggcgt gcaggttgat tctgagcatg gtctgtccga gtacgatata cacgtcgccg
    40261 gtgtaaatgc ccgaagccaa tgccagcccg acggcggcgg caaacaggac gacaatccag
    40321 actaccgttt tcatgcgcgt tctcctttag cggacggcga acgccctgcc ttttccggta
    40381 cgggctgttt ttttgcaggc tgttccggtt tgcgttccga aggcagggac ggtgcttcta
    40441 tggtcttcac atccgatgcg gcagccggtt cggaagctgt gttttcgttt gccggttcgg
    40501 aagccgcctg ttctgcttct tgattttccg ccgccgtcat gcgcgtaccg tcgcgatagg
    40561 cgcggacggc atttaggctg tttttcaaac cgtcatccgc cgtcatgcgc acatccaacg
    40621 ccttcaattc cgccagttct ttcagccacg actgcgtggc gggagacttg gcatcgaaat
    40681 actgtctgac ggcggcttcg gcattgttca aatcgccctg atagacttcg ctgttgcgct
    40741 gcattaatgc agtccgcgca tccaaaaggc ggaggcgcag gttttcacgc acaaaatatg
    40801 cctgttcggg agaaatcagc atggcatcgt tgttttccaa acggcggatt tcgaccagcc
    40861 ccttcaatgt gccgagggat ttttcccata cgttctgcca ccatgaagca gaagcggctt
    40921 cgttcttcac ctgtacgccc ggcttcaata cgccgtccaa aatcagcggc agtccggata
    40981 cggcggtttc cagcctgtcg aggcgcaatg ccgtgccgga aatatcgaca tagggacggt
    41041 ttttcagttc cgccaagtcg ctgctgaccg cctgtttgat cggcagaagc tctgcctgat
    41101 tgaaacggga caggcggctg tcgatatgct ccaatacgcc gactgccgtt tggatattgc
    41161 cggtcaacac cagctgttgc gccgccagat tgagtatggt ctcggtttcg tccaccagcc
    41221 aatcggcgcg tcctttggtc aactcgcgat aggatttttg catttccaag atttgttcgc
    41281 cgtttgcttt gacgtttccg tccaaacggt cgagttctga ttgtatggcg gcttgccggt
    41341 tgaggttgtc tttgagcagg gcggcgtttt ccgactcgcc caaggcggct ttgtcgattt
    41401 tttggttgaa tgccagctct tggtttttca agacattctg tccttggaca aacaaaaaac
    41461 cacttgcgcc caaacccaat gccgccaata ccaaggcgca ggttgccaaa gcgttgttgc
    41521 cggactgttt gacgataaaa ggagcggcag gaatgtgtac ttctgtttcg ttttctttgc
    41581 gtggggaaga ggtttcagac ggcatttctt ttgatgcctg tatctcgcgt acgggttcgg
    41641 atgatttgtt ttcaggttcg cccaccgttt tgctccttat taatttgagg ttccgggaaa
    41701 gaccatgccg tctgaaacgg aaatggaaga atgggaaagc gcggcttcca gcgtagggac
    41761 ggtttcgacc gaacacacgc cttcgcgctt caatgcctcc gcaatgcgcg gatgatgggt
    41821 aaagtatagc aaggatttga agaatcggga aaattgcggc ggaagctgcc cgaacagcaa
    41881 gcgcaccagc tcggtcgacg taatataggc ggcggcaata ttttcggttt ggaaattttg
    41941 aaagttcaaa ggtttatgcc gtctgaaata gacttctgcc acctccgtcc gaaaaccttt
    42001 ctcctgcaag gcattcatca aaaaatcccg cccgccgtgt ccgcgcacaa acaatacgcg
    42061 cgcaccttcg ggcagactgt tccaaaccgg caggcgcaaa accgcctcgc tgtcgttgcc
    42121 gtcatcaggc gcgatgaccg ttctgaccaa acagcgttcc aatgcgcggc ggctgccctg
    42181 ccctacggca atctgcgcct ttatgccgtc tgaaaggtta aggtacggga cggcggtttc
    42241 aacggcggtc ggactgaccc aaaacacggc atccgcacgg gcatattgtt cggacaaaag
    42301 ttccagtccg gcagcatctg cttcgatttc gaccggactc aatacttccg cgcgccaacc
    42361 ggcattcagg caagtttcga catcttctgc ggccctgccc gacggacgga caatcagcat
    42421 gacgggtttc gctgtttcca tcgccttatc cgccccgatt actaggaaaa atcgtcatcg
    42481 ttgaaattcg cctgcgcggt tttcggcgta cagccgaccc gtgcgacttc cttgccccta
    42541 cgctccacaa cgacaccgct gctttccgta tgagtcacgg aatcgaagcc tgtccatatg
    42601 gtgtacataa attcgccgtt ttggaatttc atcgttgccc aacgaccgct gcccatacct
    42661 tgccacctgt cggaacgccc caacaggtca gctttgctgt tgcgtatggc aatttctttt
    42721 tttgccgcac taccgaacga atattcgtaa agattgcggt tgattttttg aacttctata
    42781 tatttgttgt tgtccgtttt acaggaaaac accgtatccg cccacacggt tcgggtcgca
    42841 gccgacagaa gcaatacggc agaaaggaac atttttttca tcgtatttcc ttaatggttg
    42901 aaactccgcc atttggacat ccgtccttcg tggcagcagc atcagatgtt atttgagagg
    42961 ggtgtaaccc cttccaaatt agggcaacac atagggcaac actttatgtg tcgtcctgtg
    43021 tgttgaaaca tcattgcccg tatcctgaaa tagaaaagaa aatgcccgtt ttacggtctt
    43081 ctttccgaca aaatgccgtc tgaaacggat ttcagacggc atggtcgaaa attattgcgc
    43141 ggcaagcggc ttgagcagca aggtttcgcc gctttgcaca actttttcaa gcaggtcgat
    43201 attgggatgc ttgccgggat gggcgcgcgc gtcggcaacg tgttcggcaa accatatcaa
    43261 accctgttct gccgcttctt tgtccaattt tccgtcaaaa cgttctgcca aagcattgta
    43321 cagcttgagc gaaccgagct taccctgaac ggcgggaatg tggtgaaccg ttttgccgtc
    43381 ggcatcctga acgtccaaac cgcccagatg gtcgattgaa gaaaatgtgg cgagatagtc
    43441 ttggaaattc atatcaaatc cgaaaatcag tcagttagcg cgtgccgaaa atcttgtcgc
    43501 ccgcatcgcc caagccgggg atgatgtagc cgttctcgtt caagtggctg tcgagcgcgg
    43561 cggtgtaaat cgtaacgtcg gggtgcgcgt cgttgaccgc cttcacaccc tcgggcgcgg
    43621 caaccagcac cagtgccttg atgtttttgc agcccttggc tttcaaaagg tcgatggtgg
    43681 caaccatcga accgcccgtc gccagcatcg ggtcgataat cagggcagga cgttcgtcca
    43741 tgctgtccac gaatttctcg aagtaggaaa cgggtttcag cgtttcctcg tcgcgctgca
    43801 gtccgactac gctgattttg gcagtcggaa tcaggtcgag tacgccgtca agcataccca
    43861 aacctgcacg caggatggga acgacggtca atgttttgcc cttgatgcga tcgccttcaa
    43921 tctgaccgca ccatccgtcg ataaggtatt tttcgatttc aaaatcacgg cttgcctcgt
    43981 atgccatcag gcgcgccagc tcgatggcaa gcgtccggaa tttgtaggtg ctgcaatccg
    44041 cctccctcat cagggttaat ttgtggcgga cgagcggatg gttgataaca ttaacgttca
    44101 tcgcggtgtt ccagtgtgat tttgagacgc gccattatac ttttttttga tttgccggaa
    44161 aagaaaatgc cgtctgaaac acgttcagac ggcattgccg aatccatcag gctttcagca
    44221 gctcttgcag ctcgcctgcc tcatacattt ccatcaggat gtccgaaccg ccgacaaact
    44281 cgccgttcac ataaagttgg gggatggtcg gccagtcgct gtattcctta atgccttggc
    44341 gtacttcggg attttccaat acgttgacgg taacgtaatc ggtgcagccc gccgcgttca
    44401 ggatttgcac ggcgcgggaa gagaaaccgc attgcggaaa ctgcttcgta cctttcataa
    44461 acaatacgac gcggtgtgtc gttactactt ctttaatttg gtcgtggatg gaagccatcg
    44521 cttattcctt ttgataaagt tgacaaaatt tgtcaggtag gacgcattat acgcaaaagc
    44581 ggaaacttcc cacaatattt gccgtatatc gcgcagtatc aatgttgtga acggaaatat
    44641 tcccactcct cgacaatcct gctgtccggc aaatggcaca gggcatatcc gccgccgctt
    44701 tcgtcttttt tacttcccgt ctgccgcctt tggcaatgca gaatttggaa gcggaatttg
    44761 ccatgcctgt ccgaaacgtg ttggcaggca gccggcagca gcgcggacat acatacggat
    44821 gaattgcctt catggttttc cttatcgtcg gcacagggtc agacggtccg catatcgggt
    44881 acttttttaa acagccggta aaagttttcc gtcgtgtatg ccgcaacctg ttccaatgtt
    44941 tggttccgca atttggcgat atgttcggcg gtatggcgca caaaagccgg ttcgttctgc
    45001 ctgcctcgtt tgggaacggg ggcgaggaac ggcgcatcgg tttccaccaa aatgcggtcg
    45061 tccggcacat attttgccgc ctcctgaacc aagggtgcgt ttttaaaggt aacgattccc
    45121 gagaaagaaa tataaagccc caaatccatt gctgcacggg caaaaccgat gtcttcggaa
    45181 aaacagtgga taacgcccga attaacccgg cattctttca ggatagacaa ggtgtccgcc
    45241 gccgcatcac gcgtatggac gataacgggc agtccggttt gattggctgc ttcgatgtgg
    45301 tctgcaaagc gtttgtgttg ccaggacaaa tcgcctttgc accagtaata atccaaaccc
    45361 gtctcgccaa tgccgaccac tttcggcgag gcggctgcgg caaccatttc cgcaatagaa
    45421 aattcttcgg cttccttgct gtcgggatgt acgcctattg tgcagtaaat atgttcataa
    45481 acttcggcga tagcggatac ttcggcaaaa ctttccctgc tgacgctgat ggcgagcgcc
    45541 tgccccacgc cgtttgcttc catgttggac aaaacttcgg gcaggcgttc tttcaaacct
    45601 tcaaaattga ggtggcagtg cgaatcgatg atatgcatgg cgttacatgg taaacgtagg
    45661 tctgctgccg ccgattgtgt tgcccagttc gacttcgatc tggtctttga ctttcaggca
    45721 gttttcgtgt tttgtgaatg ccagccccac ccctttgggt ttggaggaag tacgcgcagg
    45781 attgatccag gcgactttgg tcggcaggaa cagtttgggg aagttgagga tttctacggc
    45841 aagcagaata tcgtccccga tggaaaatac gtcgttggtc tgcacaaaca gaccgccgtg
    45901 ttccaaaaac ggcatgtagg agctgtacag cagattcatg tctttcagct gcaacgacat
    45961 catttttgcc ggaatatttt gtccgtctga cataattcac ccgttctctt tcttcaattc
    46021 caaatagttg atgagcagat gctcgatctg cattttgaca tttaaagtat gaaacccgta
    46081 gggggcaagc tgtttgagca tatcctccgc cgcaaataca ttgcgcaggc ggaaaccgga
    46141 tgccgtctga agcagcctgt cttcataagc gggataatag acgggtttca tgtgttgcag
    46201 gcacaatccc aaatcgacca gccatttctg catccacccg acaaatacgt cgagcggaag
    46261 tttctcctta tcgaaaagcg cggcgtaatc caaaatcttc aacaaccttg gttctgccaa
    46321 aatatccaac agtttaatcc gcaacgcacg gacaccgtcc gcctcatcaa acagcggcgc
    46381 tccggaatgg aaagccagac gttcctcagg ttccgccaca cccctttcac gcagatatgc
    46441 caatgcctct tcatgggaag gcgcgggcaa aaccatcttc cggcagcggc ttttaatggt
    46501 cggtaaaacc ttgtccgccg cgtggctgac cagcaaaaag accacttgtg gcggcggctc
    46561 ttccaacact ttcaacaaac tgttggcggc ttggacattc atactttccg caggatgaat
    46621 cagaatcacg cgcaaaccgc cccgtaccga agtcaggtac acattatcga tgatttccct
    46681 gacggcatcg attttgatct gcaacagttt gcgtccgttt tcgggttcgt ccgacaaggg
    46741 ggtgatttcg taaaaatcgg gatggcttcc ctgtccaaac agatggcagg acatacattc
    46801 gccacagggt ttgcagcccg gtgcaggggt ttcgcacaac agtgccttcg ccgcaaagcg
    46861 ggcaaatgta gttttccccg tatctttttt gccggcaaac agccatgcat tgggacgacg
    46921 ctcccaatgt tccgcaatct gccgccattg ctcattatgc cacggataaa tcatttcaaa
    46981 ccatccgtcc ggaaatccat accgccatca gagcagttcc aaatcggggc tggaactttg
    47041 cgcgcggcgg ctgcgcaact gtaccgccaa acgcaaatcg tgtgcggaat cggcattttt
    47101 caatgcttct tgcagggaaa tatcgccttt ttcatacaat tggtaaaggt gttgatcgaa
    47161 ggtctgcata cccagggtag tggatttttt catcacttct ttgatttcat ggatgttgcc
    47221 gttgtgaatc aactccgaaa tcaggggcga attgagcagc acctcgactg ccgccaccct
    47281 gcccttgccg ccgtctcgcg gaacgaggcg ttgcgaaata aacgcctgaa ggttgagcga
    47341 caaatccgtc agcaattgtt cgcgccgctc ctcggggaaa aagttgatga tgcggtcgag
    47401 tgcctgattg gtgctgttgg cgtgcagcgt cgccatacac aaatgccccg tttcggcaaa
    47461 ggcaatggcg tagtccattg tttcgcggtc gcggatttcg ccgataagga tgacatccgg
    47521 cgcctgacgc agcgtgtttt tcaacgccgc catccagttt tccgtatcca cgccgacctc
    47581 gcgctgggtg atgatgcagt ttttgtgttc gtggacaaac tcgatcgggt cttcgatggt
    47641 gatgatgtgt ccgaacgaat tttcattgcg gtaatcgata agcgaggcaa gcgaagtcga
    47701 tttgcccgag ccggtgccgc cgacaaaaat aaccagcccg cgttttttca gcgcgacatc
    47761 cttcaagacc ggcggcaggt tcaggctttc aaacttggga atcttgctgg taatcgcacg
    47821 gaataccaac gccgtcgcgc cgcgctgtat catcgcattg acgcggaagc ggctggtgtc
    47881 cggcaggctg atggcgaagt tgcactcgtt ggtcgatgaa aattcttccg cctgcttcgc
    47941 actcataatc gaaaaggcga tttccataca tttttccgcc gtcagcggtt cgtccgtgat
    48001 gcgggtgatt ttgccgtcca gcttcattgc gggcgggaaa tgggtcgtca caaacaggtc
    48061 ggaacctttg tttttgttca tatggcgcag ccatgcgaac agttcttcct ttgcggtcat
    48121 attttcatcg gtaaacatcc tcgtccccta atcttcaaac aatctgtacc ttcccattct
    48181 accaaaaacc cgccgtgccg tctgaaaccg tttcagacgg cacggacaaa ccgcccccac
    48241 tcaatcctgc cgtgttagcg tgtataatcc ggcattctat ccgaaattca caacaggtca
    48301 gcaatatgtc tttgaaaaca gtagccgtta tcggcgcaat ggaacaagaa atcaagcttt
    48361 tgcgcgagat gatggaaaat atcaaagccg tctctttcgg cagattttcc gcctatgaag
    48421 gcgaattggt gggaaaacgc atggtgcttg cattgagcgg catcggcaag gtcaacgcgg
    48481 cggttgcaac ggcttggctt atccatcaat tcgcaccgga ctgcgtcatc aacaccggca
    48541 gcgcgggcgg tttgggcaag ggtttgaaag tcggcgacgt agtcatcggt acggaaaccg
    48601 cacaccacga tgtcgacgta accgcattcg gctatgtttg gggacaagtg ccgcaactgc
    48661 cggcaaggtt tgcttcagac ggcattttga ttgaggcggc aaaacgggcg gcgcggacgt
    48721 ttgaaggcgc ggcggtagaa caaggcctga ttgtcagcgg cgaccgcttc gtccacagca
    48781 gcgaaggcgt ggcggaaatc cgcaagcact tccccgaagt taaggcggta gaaatggaag
    48841 cggcggcaat cgcccaaacc tgtcatcagt tggaaacgcc tttcgtcatc atccgcgcgg
    48901 tttccgattc ggcagacgaa aaggcagaca tcagctttga cgagttcttg aaaacggcgg
    48961 cggcaaattc cgcaaaaatg gtggcagaaa tcgtcaaatc tttataaaac ctttatcaaa
    49021 tcatgccaaa acagcggttt tccgttagaa tatcgggcta tttccacgtt gcgcccgata
    49081 tttggacgta ccgctgtttg ttcagacggc atttctaaaa cctaccgttc agcattaaaa
    49141 acaactcaat gaaaaatatc cgaaatttct ccatcattgc ccacatcgac cacggcaaat
    49201 cgacgcttgc cgaccgcttc atccaatatt gcggcggttt ggatttgcgc gaaatgagta
    49261 cgcaggtact cgattccatg gacatcgaaa aagagcgcgg cattaccatt aaagcgcaaa
    49321 ccgccgcgct caactataaa gcacgcgacg ggcaggtgta tcaactcaac ctgattgaca
    49381 cgccgggaca cgtcgacttc tcttacgaag tctctcgttc gctctctgcc tgcgaaggcg
    49441 cgcttttggt cgttgacgcg tcgcagggcg tggaagcgca aactgtggcg aactgctata
    49501 ccgcgattga tttgggcgtg gaggttgtgc ctgttttaaa caaaatcgac ctgcccgccg
    49561 ccgaccccga acgcgtggaa caagaaatcg aagacattat cggcatcgat gccgtcggtg
    49621 cggtgcaatg ttccgccaaa agcggcatcg gcgtggaaga cgttttagaa gaaatcgttg
    49681 ccaaaatccc tgcgccgacc ggcgacgaaa acgcgccgct gcaagccgtg atcgtcgatt
    49741 cgtggtttga caactatgtc ggcgtggtca tgctgattcg agtgaaaaac ggcaccatca
    49801 aactgaaaga caaagtgcgc tttatgagca ccaaggcgga aacgcaggtc gagcagctgg
    49861 gcgtattcac accaaaatca gttcaaaaac aagaactcaa agccggcgaa gtgggctttt
    49921 tgattactgg cgtaaaagaa ttgggtcagg cgaaagttgg cgatacggtt acactggttg
    49981 ccaaccctgc cactgagccg ctgcccggct tccaagaagt acaaagccaa gtattcgccg
    50041 gactttatcc cgtggaaagc cacgactacg aagccttgcg cgacgctttg gaaaaattgc
    50101 agcttaacga cgcttcgttg aaattcgagc ctgaagtttc ccaggcattg ggcttcggct
    50161 tccgttgcgg cttcttgggt ctgctgcact tggaaatcgt gcaggaacgt ttggagcgcg
    50221 agttcgacat ggacttgatt accaccgcgc cgacggtggt ttacgaagtc gtgttgaaaa
    50281 acggtgagaa aatcgaagtt gaaaacccgt ccaaactgcc cgacatcggc agcatcgaaa
    50341 ccatactcga gccgattatt accgcgacca tcctcgtgcc gcaggaatac gtcggcaacg
    50401 tcatgacttt gtgtaaccaa aagcgcggcg ttcaggtcaa tatgcaatat atgggccgcc
    50461 aagtcatgct gacttacgat ttgcccatga acgaagtcgt catggacttc ttcgacaaac
    50521 tcaaatccac ttcgcgcggt tatgcttcgt tggactacca tttcaaagag ttccaaccgt
    50581 ctgatttgat taagctggat atcatggtca acggcgaaaa agtcgatgcc ttaagcctga
    50641 ttgtgcaccg tcaaagcgcg gttcaccgag gccgcgagct ggcatcgaaa atgcgcgagc
    50701 tgattccgcg ccaaatgttc gacattgccg tccaagccgc catcggcagc cagattatcg
    50761 cccgcgaaaa cgtcaaagca ctgcgtaaaa acgtcttggc gaaatgttac ggcggcgata
    50821 ttacgcgtaa gaaaaaactg cttgaaaaac aaaaggcagg taaacgccgt atgaaacaag
    50881 tgggcaatgt ggaaatcccg caaagcgcgt tcttggcgat tttgcaggtg agcgacaaat
    50941 aatatttcgg cacatagggc ggcgcgtcgg gaacacctct caaaccgccc tgatttcagg
    51001 ggcacgcgcc cgcaaacaaa ttttgcaatt tgccgcctta aaacggttgc acggccgggc
    51061 gggtttttaa ccgttgagga acaacaatga acacaatgct aatgtcgggc gcggctgccg
    51121 cgctgcttgc cggcatcatc ctttatttca aaagcgacaa gaagcggcag gaaaacggtg
    51181 aatggagttc cggcctcgaa tacgcctata tcctgacggc ggtcggcgtg tttgccgctt
    51241 tgtccctgtt tatgagcttt gccgccgttt tcctgatttt cgttgtattg tgcggtacgg
    51301 cttgggcggt atacaaatac cgcctgaaga ctcatcccga aatctcggaa agcagccact
    51361 tcggcgatta tttcggcagt ttcttcccta ccgttttggt attgttcctc atccggtcgt
    51421 ttattgccga accgttccaa atcccgtcca gctcgatgcg cccgggcctg attaagggcg
    51481 atttcatttt ggtcggcaaa ttttcctacg gtctgcgcgt acccgtttta aacaatatat
    51541 ttattcctac aggcaaaatc gaacgaggcg atgtcgttgt ttttaattat cctctgcagc
    51601 cggagatgac ctacatcaag cgtattgtcg gcattccggg cgatgtggtc gaatatcggg
    51661 ataagatttt gacggtaaat ggcaaaccca cttccgatat tcctaacggc acataccgtt
    51721 atcccgacga caccgaccct tccgaaatcc acaacacgga tatgttccgc agcgatctgg
    51781 acggcaaatc cttcaatatt ctgaaaaaag aaggacagcc tgccgtttcc ctgcccgtat
    51841 tgggcaaata tacctccgat attatgtctg aaaacggata ttccatagag caaagcggtt
    51901 tggaacactg ccaatatgcc gacgacggca gcggtttcgt gtgcaaagtt cccgaaggac
    51961 gctatttcgc tatgggcgac aaccgcgaca acagtgccga ttcgcgctac tggggatttg
    52021 tggatgacaa gctggttgtc ggcaaggcaa tgttcatttt gatgaacttc ggcgatttcg
    52081 gcaggtccgg tacggcaatc cgttaggccg gaaaattacg ccggaacatc aaaaatgcca
    52141 tccgaaccct gtttcagacg gcatttttat catcaaacgc cttgtttccc tgccggcagc
    52201 ctcggggata atatagtgga ttaaatttaa tccactatat ttccgtttgt gtggaaaccc
    52261 atcccattga ataaagggca tttcgtccga ctgacggtgt gggcggcata tggaaatacg
    52321 gacattattt tagttgcatt gccattccag cggaggcggc ttttttgatt ctgcccgccc
    52381 cgatgccgtc tgaaagggct tcagacggca ttttccgctc cgcgccgttg ctggcgtgtt
    52441 tggtcttgcc ggttttgact gcctgccagt tcaatcgtac gcaggtttgc cacggcagaa
    52501 aatctgatgc cggacggagt caaggctcgg actgctgttt cagcgaaccg aggtaaattg
    52561 ccgaaagcgt cagcgccacc cctgcccact gcacgccttc aatcggtttg cccaacccga
    52621 aataatcgat gaacagggcg gcaaccggtt cggacaaaag cagcagcccc gtcagcgaca
    52681 gcgaaagcag cggaatcgca taggcaacca tcgcccacgc gaagcactgc atcaccacgc
    52741 cgtacacaag caccaaaccc gcatctttcc aagtcgtcgg ataaagcacg ccgccatcca
    52801 tcagcaatgc cggaacaacc agcgataccg cgccgcccaa actcaaaatc atcattgaag
    52861 ggaaaagcgc caccggctcg atttcatggg ttttgcggac gaacaccatc gacagggcga
    52921 gcatcagtcc cgataccaaa ccgctggcga atccccaaac cgcattaccg ttgtagccga
    52981 attccgcacc ggcaatcatc gccacgccgg caactgccga tattaagcct gcctttttca
    53041 gcccgctcaa acgttcgtta aaaaagaaaa caccgattgc cgacaagaaa aagatttgca
    53101 ggctgttgag cagggtggaa atacccggcc cgaccgcgtg tatgctttcg tgccacaacg
    53161 ccaaatcgaa agcgaggaac acgcccgccg tcagggcata tcggacggtt ttcctgtttt
    53221 ttgggaattt ttgcccgaaa aaccgtgcca aaaaccaaaa tacgaacacc gaaatcagca
    53281 accgccaaaa tgcgattgca tacgggccga cggggacgga cctgacaatc agactgccca
    53341 gaccgaacac cacgcaaccc agcaccagca atggtgcggc atattttttg ccgttaccga
    53401 ccacaatatc ccctcccttc cccatacggc tgccgcgccg acggcaagaa tgccgccatt
    53461 ccgaaaatat gccgtctgaa gcccaaagcc ccggctgcac ccggacaaac ggctgatttt
    53521 acgggcaggc cgactatttt ccgttggggc aaaaaaccgt cctgccagat aaaacccgaa
    53581 tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt
    53641 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
    53701 cgttgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcaatgcg
    53761 gcggcaccaa tgcctgatta aaacacaacc ggcgttttcg gcatatatgc aaaaacgcat
    53821 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
    53881 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
    53941 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
    54001 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
    54061 gggcggtttc aaaagccgct tcgttcgaca ctttggtaat gctgtcgtaa attttggtat
    54121 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
    54181 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
    54241 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
    54301 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
    54361 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
    54421 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
    54481 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
    54541 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
    54601 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
    54661 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
    54721 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
    54781 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
    54841 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
    54901 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
    54961 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
    55021 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
    55081 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
    55141 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
    55201 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
    55261 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaaa
    55321 agcccgagca gcggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
    55381 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
    55441 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
    55501 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
    55561 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
    55621 aatacgccaa ctcgccttca aacaccgttt ccgcagggcc ggtcatcatc acatcgccgc
    55681 cgcaggccca ttcgatatat aaagttccgc ccggcaaaat cacctctacc gttttccctt
    55741 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
    55801 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
    55861 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
    55921 cggtttcgcg cacctgcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
    55981 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
    56041 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacttcggtt
    56101 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata atgcccttgg
    56161 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
    56221 aacaacgcgc tccgttgccg cattgcccga cctcgctgcc gtcggcattg aaaatacggt
    56281 aacggaaatc cacaccttcg gtttccgaac gcccgaccac caaaagctgg tcgaacccga
    56341 tgccgcggaa acggtcagcc catgctgcaa tcggtgcatg ctcaggaata aaatcctgac
    56401 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttagta aatttcaaag
    56461 tgttcatatt attctgaaat cccgtattat ctgttgcttc cgaattccga aatcccccgc
    56521 accgaaggga aatgcttcca tctcccgccg cctgaggatt aaaatcagcc attatactgt
    56581 ttgttacccc ttacaagaaa caccatgttc cgcatattac tgttgctttt cccttgtgca
    56641 atccttgtcg cctgcacccg aaacataccg gcaaacccga ccgtttcaaa ttgcgcgcat
    56701 cttcaaaacc gcaccatcct gtattttgac gcacacggca aacccgccga cggcgcagta
    56761 aagggcggat tttacaaaat tatcgaaata cagggtgcaa actgcatcct tattcaagac
    56821 tttaacagca cgggcaatgc cctcggcgga tcctatgtgc cggactgccg caaatcatcc
    56881 cttttcccga aaaatatttg acttcaaact gcctatgaac cacgacatca ctttcctcac
    56941 cctgttcctg ctcggcttct tcggcggaac gcactgcatc ggcatgtgcg gcggattaag
    57001 cagcgcgttt gcgctccaac tccccccgca tatcaaccgc ttttggctga tcctgctgct
    57061 taacacagga cgggtaagca gctatacggc aatcggcctg atactcggat taatcggaca
    57121 ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat attttataca cggccgccaa
    57181 cctcctgctg ctctttttag gcttatactt gagcggtatt tcttccttgg cggcaaaaat
    57241 cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg atactcaacc ggctgttacc
    57301 cataaaatcc atacccgcct gccttgcggt cggaatatta tggggctggc tgccgtgcgg
    57361 actggtttac agcgcgtcgc tttacgcgct gggaagcggt agtgcggcaa cgggcgggtt
    57421 atatatgctt gcctttgcac tgggtacgct gcccaatctt ttggcaatcg gcattttttc
    57481 cctacaactg aaaaaaatca tgcaaaaccg atatatccgc ctgtgtacgg gattatccgt
    57541 atcattatgg gcattatgga aacttgccgt cctgtggctg taagccccat tcattcccga
    57601 tacccgtaaa aaataccgtc tgaaacctgt tcagacggca ctttcaccct aagcgttact
    57661 cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc tttgatggac aggcgcacac
    57721 ggcctctgtc atccacttcc aatgctttca cgttcaccac ctgaccgact tgcaggtagt
    57781 caccgacatt gcgtacgcgc tcgtgggcga tttggctgat gtgtaccaaa ccgtctttgc
    57841 ccggcatcac gctgacaatc gcgccgacgt tgttgtcgag gattttcacc acagtgcctt
    57901 catacacttt gcccacttcc acttcggcag taatctgctc gatacgtttt ttcgccgcat
    57961 cgccggcttc ttgagtggtc gcagcaatgg taatcgtacc gtcttcggca atattgattt
    58021 ccgtaccggt ttcagcggta atcgaacgga tggtttcgcc gcccttaccg ataacttcgc
    58081 ggattttgtc ttggttgatt ttcatcgtaa acaaacgcgg cgcgtgtgcg gacaactctt
    58141 gcgggcccgc aacggcggct ttcatctgat ccaagatgtg cagacgcgct tctttggcct
    58201 gtgccaaagc gatttgcata atttctttgg taatgccttg gattttgatg tccatttgca
    58261 gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc catatcgccc aagtggtctt
    58321 cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc ttccagaatc agacccatcg
    58381 cgataccggc aacgtgtgct ttcaaaggca cgccggcaga cagcaggctc aggcagccgc
    58441 cgcagacgga agccatagag gaagagccgt tggattcggt aatttcggag accacgcgca
    58501 tggtgtagct gaaatcttca ggtttcggca atacggccaa caatgcacgt ttagccaaac
    58561 ggccgtgacc gatttcacgg cgtttcggcg cacctacgcg acccacttct ccggtagagt
    58621 acggcggaaa gttgtagtgc agcatgaagc ggtcggtgta ttcgccggac agcgcgtcaa
    58681 tgatttgctc gtcgcgcgaa gtacccaaag ttgcaaccgc caaagcttgg gtttcgccac
    58741 gggtaaacaa tgcggaaccg tgtgtacgcg gcaatacgcc ggtttggatg ttcaaaggac
    58801 ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttgacc gtccaaaatt tggctgcgga
    58861 caacatcggc ttccaagtgt ttgaaaatac ctttgatttc gttggcggcc aaagtatcgg
    58921 tttcttcggt aatcaaggct tcttttaccg cattccaagc ctcgtccaat ttggcggaac
    58981 gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt ttcgccggca atcccgcgga
    59041 ctttggcaac taattcttca ttggtttcag gtgctttcca atcccaaact tccggattga
    59101 cttcgtcggc aaattcattg atggcgttaa tcgccgcctg catttgatcg tggccgtaaa
    59161 ccaccgcgcc caacatcacg tcttcgggca ggattttggc ttcggattcc accatcaaca
    59221 cggctttgga cgtaccggcg accaccaagt ccaattgcga tttagccaat tcggctttag
    59281 tcgggttcaa aacgtacacg ccgtttatat aacccacgcg tgccgcacca atcggaccgg
    59341 caaacggtac gccgctcaac accagcgcgg cagacgcgcc caacattgca ggaatgtcgg
    59401 aatcgatttc gggatcgacg gacacgacca tcgctacgat ttggatgtcg tggtagaaac
    59461 cttcggggaa cagcggacga atcgggcggt cgatcagacg gctggtcagg atttcttttt
    59521 cgctttgttt gccttcgcgt ttgaagaaac cgccggggat tttacctgcg gcgtaagtgc
    59581 gttccaaata atcgacggtc agggggaaga agtcttgacc ttctttcact tctttgttgg
    59641 tggtaacggc aaccaaaaca acggtgtcgc ccatagagac tttaacggca gcggcggctt
    59701 ggcgggcaat ttcgccggtt tccaaagtaa cggtgtggtt gccgtattgg aaagttttaa
    59761 cgtgtttgtc gaacattatt gttcctttca aaataccgca ctgctaaaac actaataatg
    59821 cacactaaaa tccgaatgtg catagttagg gtttcagacc gtgcggcagg ttgtaaacaa
    59881 gctttcagac gacatttcgg acgctgaaag caattgcgga ttataaaggc aaccatcctt
    59941 aaaatccagt attgatacaa taaaaaggcc gtctgaaaca tatctctttc agacggcctt
    60001 caaacttaaa aatcaatcct gagtcagcaa agtcaatgct tcccgatact tctcgacagt
    60061 tttctgaatc acatcggcag gcactttcgg cgcaggggct tttttgttcc aaccgctttg
    60121 ttccagccag tcgcggacga attgtttgtc aaaagacggc ggattggtgc cgacttcata
    60181 ttgatccgcc ggccagaagc ggctcgaatc gggagttaat acctcatcca tcagcgtcag
    60241 cgtaccgttt gtatccaaac cgaattcaaa tttggtatcg caaataataa taccgcgcga
    60301 tttggcatat tccgccgctt cggtgtaaag ccgaaccgcc ttggcgcgca cttcttccgc
    60361 caattctttg ccgataatgc gtccgcattc ttcaaagctg atgttttcat cgtgatcgcc
    60421 gactgcggct ttggtcgatg gcgtaaaaat cacttcaggc agttgttgcg cttcctgcat
    60481 accttcgggc agccgaatac cgcaaaccga gccggttttt tgataatctt tccaaccgct
    60541 gcctgccaga taaccgcgca caatcgcctc tactttcacc ggagtgagct ttttagccac
    60601 gacggcgcgt ttttctaaaa ctttggcttc gttttcaggc aaaacatcgt aaaccgtttg
    60661 accggtaaag tggttgggca taatatgcgc cagtttttta aaccaaaaat tggaaatctg
    60721 cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa atcacatcaa acgcggacag
    60781 gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt tcatataaat cgcgcacttt
    60841 tccagaatag atctttacca aaccaatctc actcatttcg ccccccctga aaatatcttg
    60901 aaaataccga cccgacaccc gacaggtttg aatcgcaaac cgatattcta gccgaagtcg
    60961 gcgcaaaaca atacccatgg cacaaaaagc caacccgtca accgtcggca aagttttggc
    61021 actataatac cgacagcaag tcctacaata cacttttacc aaaggaaata cctcatgaga
    61081 atcctattga caggctcgaa aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa
    61141 gactgggaaa ccattgcgac ggattccgca tccctagaca ttaccgatgc cgatgccgtc
    61201 tgcaacatgg tcaaaagttt ccaacccgac gccattgtca acacggctgc ctatactgcc
    61261 gtcgacaaag cggaaggcga tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac
    61321 aaccttgcct tggcagcaca tcgcgcccat gcccgattca tccacatctc aaccgactat
    61381 atctttgacg gtaaagggaa aaggccctat caggaaagcg actttaccaa tccttccaat
    61441 gtatacggac aatccaaaac cgccggcgaa ctgctcgcac tgtctgccaa tcccgacagc
    61501 cttatcctgc ggacttcttg gctgtttagc gaatacgggg acaactttgt ccgcaccatg
    61561 ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc acaaccaaat cggctgcccg
    61621 acctatgccg gcgacctggc tgccgccatc atccgccttt tgcagcaaac gtcgcccgtg
    61681 cgcggcattt accactacgc cggcggcaaa tccgtatcct ggtacgaatt tgcccaacat
    61741 attttccaag cggcatcgca acagcagaca tccttccccg ttcccgaatt gactgccgtt
    61801 tcagacaagg aatatccgac cgccgccccc aggcccgcat acagcatttt ggactgccgc
    61861 aaaatcgaaa acgacttcgg catcaaaccg tcagactggc agaaagccct tgcacaggtc
    61921 gtttccaagc tgctctctag tgggtactag atataatgca gcatccgata aagtaaagcc
    61981 ccgaagtggc agcttcgggg cttatcggat agaaaggagg cttgaagaaa tggcgaaagt
    62041 tattttattc ctgtttcttt t
//
LOCUS       DAXIHS010000003        41791 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXIHS010000003 DAXIHS010000000
VERSION     DAXIHS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.003"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            322..1254
                     /gene="pip"
                     /locus_tag="ACFOF2_000127"
     CDS             322..1254
                     /gene="pip"
                     /locus_tag="ACFOF2_000127"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC7106521.1"
                     /translation="MYEIKQPFYSGYLQVSEVHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIEKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENQRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILTRLV"
     gene            complement(1404..2600)
                     /gene="bamC"
                     /locus_tag="ACFOF2_000128"
     CDS             complement(1404..2600)
                     /gene="bamC"
                     /locus_tag="ACFOF2_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC7106522.1"
                     /translation="MPSEPFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKTTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYADKDASTLLGK
                     LHSELR"
     gene            complement(2609..3484)
                     /gene="dapA"
                     /locus_tag="ACFOF2_000129"
     CDS             complement(2609..3484)
                     /gene="dapA"
                     /locus_tag="ACFOF2_000129"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC7106523.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIESGTDGIVAVGT
                     TGESATLSVEEHLSVIEETVKHVNKRVPVIAGTGANNTLEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3862..5253
                     /locus_tag="ACFOF2_000130"
     CDS             3862..5253
                     /locus_tag="ACFOF2_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC7106524.1"
                     /translation="MAETMKKQADSPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(5315..6067)
                     /gene="rlmB"
                     /locus_tag="ACFOF2_000131"
     CDS             complement(5315..6067)
                     /gene="rlmB"
                     /locus_tag="ACFOF2_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC7106525.1"
                     /translation="MANQRLIYGFHAVNARLWQNPKSITELYIQEGKNDARTREVLEK
                     AANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCVLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(6134..6622)
                     /locus_tag="ACFOF2_000132"
     CDS             complement(6134..6622)
                     /locus_tag="ACFOF2_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC7106526.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGILGVKYTGAGRLAVRGAFIGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVIGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6627..7346)
                     /gene="tadA"
                     /locus_tag="ACFOF2_000133"
     CDS             complement(6627..7346)
                     /gene="tadA"
                     /locus_tag="ACFOF2_000133"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC7106527.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQTGAVQTFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAIIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(7350..8291)
                     /gene="miaA"
                     /locus_tag="ACFOF2_000134"
     CDS             complement(7350..8291)
                     /gene="miaA"
                     /locus_tag="ACFOF2_000134"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC7106528.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTTALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTHLIEAAKRFFGV"
     gene            8645..8923
                     /locus_tag="ACFOF2_000135"
     CDS             8645..8923
                     /locus_tag="ACFOF2_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106529.1"
                     /translation="MFRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKHQTMFLFIPIVLLVVYLFHYFGAF"
     gene            8942..10093
                     /gene="earP"
                     /locus_tag="ACFOF2_000136"
     CDS             8942..10093
                     /gene="earP"
                     /locus_tag="ACFOF2_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC7106530.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLH
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCE
                     AVRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWQQAGSPMTLLLAGTQIID
                     SLKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     AQLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRRLSDDLNGGEALSA
                     TQRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            10136..10696
                     /gene="efp"
                     /locus_tag="ACFOF2_000137"
     CDS             10136..10696
                     /gene="efp"
                     /locus_tag="ACFOF2_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC7106531.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(10746..11582)
                     /locus_tag="ACFOF2_000138"
     CDS             complement(10746..11582)
                     /locus_tag="ACFOF2_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC7106532.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPNKNDKTKEVGASAASSSASSAS
                     SQSDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(11699..12280)
                     /gene="metW"
                     /locus_tag="ACFOF2_000139"
     CDS             complement(11699..12280)
                     /gene="metW"
                     /locus_tag="ACFOF2_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC7106533.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTSNRQVKHFPNLLGSLAFYRVG"
     gene            complement(12277..13416)
                     /locus_tag="ACFOF2_000140"
     CDS             complement(12277..13416)
                     /locus_tag="ACFOF2_000140"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC7106534.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            13566..13766
                     /locus_tag="ACFOF2_000141"
     CDS             13566..13766
                     /locus_tag="ACFOF2_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106535.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            complement(13978..14103)
                     /gene="ykgO"
                     /locus_tag="ACFOF2_000142"
     CDS             complement(13978..14103)
                     /gene="ykgO"
                     /locus_tag="ACFOF2_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC7106536.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(14103..14378)
                     /locus_tag="ACFOF2_000143"
     CDS             complement(14103..14378)
                     /locus_tag="ACFOF2_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC7106537.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            14578..15456
                     /gene="metF"
                     /locus_tag="ACFOF2_000144"
     CDS             14578..15456
                     /gene="metF"
                     /locus_tag="ACFOF2_000144"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC7106538.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15594..17870
                     /gene="metE"
                     /locus_tag="ACFOF2_000145"
     CDS             15594..17870
                     /gene="metE"
                     /locus_tag="ACFOF2_000145"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC7106539.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTGFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKNQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            18149..18886
                     /locus_tag="ACFOF2_000146"
     CDS             18149..18886
                     /locus_tag="ACFOF2_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC7106540.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            19135..20538
                     /locus_tag="ACFOF2_000147"
     CDS             19135..20538
                     /locus_tag="ACFOF2_000147"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC7106541.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDSFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAS
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            20817..21194
                     /gene="sdhC"
                     /locus_tag="ACFOF2_000148"
     CDS             20817..21194
                     /gene="sdhC"
                     /locus_tag="ACFOF2_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC7106542.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            21188..21529
                     /gene="sdhD"
                     /locus_tag="ACFOF2_000149"
     CDS             21188..21529
                     /gene="sdhD"
                     /locus_tag="ACFOF2_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC7106543.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            21532..23295
                     /gene="sdhA"
                     /locus_tag="ACFOF2_000150"
     CDS             21532..23295
                     /gene="sdhA"
                     /locus_tag="ACFOF2_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC7106544.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            23416..24123
                     /locus_tag="ACFOF2_000151"
     CDS             23416..24123
                     /locus_tag="ACFOF2_000151"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC7106545.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITSERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            24127..24375
                     /locus_tag="ACFOF2_000152"
     CDS             24127..24375
                     /locus_tag="ACFOF2_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC7106546.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            24487..25770
                     /gene="gltA"
                     /locus_tag="ACFOF2_000153"
     CDS             24487..25770
                     /gene="gltA"
                     /locus_tag="ACFOF2_000153"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC7106547.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            25975..28803
                     /locus_tag="ACFOF2_000154"
     CDS             25975..28803
                     /locus_tag="ACFOF2_000154"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC7106548.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            28903..30084
                     /gene="odhB"
                     /locus_tag="ACFOF2_000155"
     CDS             28903..30084
                     /gene="odhB"
                     /locus_tag="ACFOF2_000155"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC7106549.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(30136..30252)
                     /locus_tag="ACFOF2_000156"
                     /pseudo
     CDS             complement(30136..30252)
                     /locus_tag="ACFOF2_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            30449..31882
                     /gene="lpdA"
                     /locus_tag="ACFOF2_000157"
     CDS             30449..31882
                     /gene="lpdA"
                     /locus_tag="ACFOF2_000157"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC7106550.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELLTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            31977..32264
                     /locus_tag="ACFOF2_000158"
     CDS             31977..32264
                     /locus_tag="ACFOF2_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106551.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            32318..33529
                     /gene="sucC"
                     /locus_tag="ACFOF2_000159"
     CDS             32318..33529
                     /gene="sucC"
                     /locus_tag="ACFOF2_000159"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC7106552.1"
                     /translation="MEANSGHYTNLKEKSMNLHEYQAKELLAGYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            33540..34430
                     /gene="sucD"
                     /locus_tag="ACFOF2_000160"
     CDS             33540..34430
                     /gene="sucD"
                     /locus_tag="ACFOF2_000160"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC7106553.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(34670..34993)
                     /locus_tag="ACFOF2_000161"
                     /pseudo
     CDS             complement(34670..34993)
                     /locus_tag="ACFOF2_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(34965..35108)
                     /locus_tag="ACFOF2_000162"
     CDS             complement(34965..35108)
                     /locus_tag="ACFOF2_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106554.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIGFAYGLYGCAKT
                     ISL"
     gene            complement(35394..36596)
                     /locus_tag="ACFOF2_000163"
     CDS             complement(35394..36596)
                     /locus_tag="ACFOF2_000163"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC7106555.1"
                     /translation="MLIPQTSLFDQENNRIISQRKLLEQSLLQGIPLNELIRQNQMSY
                     DDLIYIHHDKKPFEKRKNLVLSDFQKKENHIPIISFFSGAGGLDLGFEAAGFQHSMLI
                     EKIPLFCDTLRLNRPNWSVFNADVSETEEMVELIRNNIGSLSFDGVFIGGPPCQPFSI
                     ASNQRFSKTDKNFKRVGFEHKQNGNLLFNYVELIKIFRPKVFLIENVSGLMDIDNGKQ
                     LASVYCDLQQQGYSLYKPQIINSEYFHVPQQRIRLFIIGYLGDKKFSPLQPTNTPLAC
                     GNTFEIPIINQHNHITREHSAESILRYMELAYGQRDKKGRVDRLDPSKPSKTIIAGGT
                     KGGGRSHLHPYIPRTLSVRESARLQTFPDDYIFTGAVARQFTQVGNAVPPILAAQLAT
                     AIKHSYFE"
     gene            complement(36609..37793)
                     /locus_tag="ACFOF2_000164"
     CDS             complement(36609..37793)
                     /locus_tag="ACFOF2_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC7106556.1"
                     /translation="MIEWRFDQGRLDYFRFDEIKKIAKGLYGINQCPKPRAKDIDIVR
                     LSLTQETNLPFAPNSSQYPVWRNYGRVFKCLLLATEHNGLICTTDLCADLVQNDEISC
                     DDYLLHFATHFSYPSPAFSGYQPSENRVFPVLAIIRALISKYKETGVNFLDMDEIIYK
                     IASNNSITGIASFSFSQLIMNKEAIKYDPRQIRELVKFISQFSFLSWYENKLFLDITD
                     FSELHLLENQFIADTQPQERLDSLEIMRLGKTNNNFLLMNEIVSLSIDDQAFSEGGKI
                     KVTHLITERNSILRKIYFSKTQNAQICRMCGLDTQRSYEWSEHIIEVHHLLPLSSPVR
                     VEDKTTSLKDLVGICPTCHRATHKYYTFWLNLHKKKDFVSYEEAYHVYEEAKSKVKRI
                     YK"
     gene            complement(37835..40684)
                     /gene="uvrA"
                     /locus_tag="ACFOF2_000165"
     CDS             complement(37835..40684)
                     /gene="uvrA"
                     /locus_tag="ACFOF2_000165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC7106557.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSITGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDSGGRIIAQGSPEEVAKVKGSYTGKYLKVVLG"
     gene            40839..41636
                     /locus_tag="ACFOF2_000166"
     CDS             40839..41636
                     /locus_tag="ACFOF2_000166"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC7106558.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         9968 a        10050 c        11124 g        10649 t
ORIGIN      
        1 aaatgaccga aattgaacgg actggattcc cgcttttgcg ggaatgacgg cgacagggtt
       61 gctgttatag tggatgaaca aaaaccagta cggcgttgcc tcgctttagc tcaaagagaa
      121 cgattctcta aggtgctgaa gcaccaagtg aatcggttcc gtactatctg tactgtctga
      181 ggcttcgccg ccttgtcctg atttttgtta attcactata tcgcgatttt tcggaatttg
      241 cctttcgggg cggcttgtgt tttgtgcggg atgtcgcgtg tcgggatgtt cggattgcca
      301 gaagcaatat gggagaagat gatgtatgag ataaaacagc ctttttatag cggatacttg
      361 caggtgtctg aagttcatca aatttattgg gaggaatcgg gcaatcccga cggtgtgccg
      421 gtcatttttt tacacggcgg gccaggcgcg ggggcttcgc ctgaatgtcg gggttttttc
      481 aatcccgatg tgttccgcat cgtcattatt gaccagcgcg gttgcggacg ttcgcgcccg
      541 tatgcttgtg cggaagacaa tacgacttgg gatttggtgg cggatattga aaaagtccgt
      601 gaaatgctgg gtattgaaaa atggctggtg ttcggcggtt cgtggggcag cactttgtcg
      661 ctggcttatg cccaaaccca tccggaacgg gtaaagggat tggtattgcg cgggatattt
      721 ttgtgcaggc cgtctgaaac ggcgtggctg aacgaggcgg gcggtgtgag ccggatttat
      781 ccggaacaat ggcaaaaatt tgtcgcgccg attgctgaaa atcagcggaa ccggctgatt
      841 gaggcgtatc acgggttgct gtttcatcaa gatgaagaag tgtgcctgtc tgccgcgaag
      901 gcttgggcgg attgggaaag ttacctaatt cgtttcgaac ctgaagaagt agatgaagat
      961 gcttatgcct cactggcaat cgcgcgtttg gaaaaccatt atttcgtcaa cggcggttgg
     1021 ttgcagggcg ataaggcgat tttgaacaat atcggcaaaa tacggcatat cccgaccatt
     1081 atcgtacagg ggcggtatga tttgtgtacg ccgatgcaga gtgcgtgggc gctgtcgaaa
     1141 gcctttcccg aagcggagtt gagggtagtt caggcggggc attgtgcgtt cgatccgcct
     1201 ttggcggatg cgttggttca ggcggttgag gatattttga cgcgacttgt gtagcgcgtt
     1261 tttgcgctta cttccgtacg ggcaggtttg cttcggcagc gattggaaaa tacagtcagg
     1321 ccaagcgtcc ggattgacga aacctgccgt ctgatgcaaa aaaagcagct tctttacggg
     1381 aagctgcttt tgttttgaac ggtttaacgc agttcggaat ggagtttgcc caacagtgtg
     1441 gatgcgtctt tgtcggcata tgcgctgccg tctttgttga gcaggacgat gcgcgagccg
     1501 ttggcgacag gctcggcata gacaatcagt tccggctgtt cggcaggttt ctccgctttg
     1561 cctttgccca gcaggcgttt gaacaggccg ggtttttgtt cggtaactgc attgctttcg
     1621 ttcggggctt tttgaaccag gaaagcgtgg cgttcggtgt tttgaccgac gacggtcagc
     1681 ccgatgcggt cgagggcgag cgcggtgcgc cgccagtttc tgccgtagtc gccaaagaca
     1741 atcaggcttt tgccttcgat acgcgccatt tcgttggcgg cgggaagggt agtttttttt
     1801 gccgatgcgt tttccgcctg ctgtccgtca acgcccaaat attgcataaa gcgcgtcagg
     1861 aaagcggctt cgaggttggg atcggacggg gagggctgcc atacggtcgt gtctttgtct
     1921 ttgccgccgt acacttcttt catggctttg tgggcgaaga agatgtcgga aacgccgttt
     1981 ttgccctgtt cgatacggac gatgaatttg tcgcgctcgc cggtggagta gatgccgccc
     2041 aaaccgactg tgtcgaatag gcggcgcaag ctgtcttggg ggattttggc acggttttcc
     2101 gcccactcgg tttccatttg tccgatggcg ggttcttcgg atttgatgtc gaagccgttt
     2161 tcctgccaaa aggctttcag gagcggccag atttcggcat gagacttgcc gtcgacaacg
     2221 agccagcgtt ggctgccgtc gcgctcgagg cggacacctt tgacgctttt caatacttcg
     2281 gcatcggcag gctgttggac ggcgggtgtg cggcgttttt ccaaatcgct ggcgcggacg
     2341 gcgcccgaac cggcaggcag gcggtagagg ttgccttggt cggggttgtt caaatcaggt
     2401 gggacttcga gtttgatcag gcggtgcgac cggctttggt agtcgagctt gggctgttcg
     2461 gttttgctgc cggagcaggc ggcaagcccg atgagtgcga gcgcggcaat gacgggtttg
     2521 atatgggtca tcgtgtcatc ctgtgtgatg gatattaaag tgtttgttgc gttatgccgt
     2581 ctgaacggtt cggacggcat ggctatattt aaagttgtcc tgaggctttc agggcggcgc
     2641 ggacttttgc ctgtccgcct tccgtcagcg gaacgagcgg caggcggacg tgcggttcgc
     2701 atctgcccaa ggcggatacc gcccatttcg gtgcggcggg gctgggttcg cagaacatgg
     2761 tgtcgtaaat cggaatcagc cggttgttga gttcgcgtgc gagggcaatg tcgacttgca
     2821 gcgcggcgcg gcacatatcg gcaaagagct tgggcgcggc gttggcggct acggtaatca
     2881 cgccgtgtcc gccgcacagc atgaacggca gggcggtatg gtcgtcgccg gaaaggacga
     2941 cgaagccttc gggcgcgcgg ttgatgagtt cgatgttgct gccgatgttg ccgctggctt
     3001 ctttcacgcc gacgatgttg gggatttcgg caaggcgcag gatggtttcg ttgttcatgc
     3061 tgacgacggt tctgccgggt acgttgtaga taatcatcgg aatcgaagtg gcttcggcga
     3121 tggttttgaa atgttggtaa atgccttctt gggagggctt gttgtaatag gggacgacgg
     3181 agagagtgaa gtctgcaccg gctttttcgg cggcttgcga aagtgcgatg gcttcaaggg
     3241 tgttgttggc gccggtacct gcgatgacgg ggacgcgctt gttgacgtgt ttgaccgttt
     3301 cttcgattac gcttaaatgt tcttcgacgg agagggtggc ggattcgcct gtcgtgccga
     3361 cagcgacgat gccgtcggta ccgctttcga tatgccagtc gatcaaatcg cggagttgtt
     3421 cgtaatggat gctgccgtct tgattcatcg gggtaatcag ggcaaccaag ctaccttgta
     3481 acatacggaa accttttatt cattgagtga ggtcgattat gcttcggatt gtagcttact
     3541 ttgttgtttg tgtgaaacat atcggtcgca cggttggttc taaggcttga gttctatcat
     3601 atcttaataa ataatatttt ttataatcat gaaattattg ggtttaagca gtggatgcgc
     3661 ggttgaggat taaaatgtgt taagcgcatg aaggccctct gaaaatgaag gactgtccgg
     3721 tgcggaaatt caaagaaatt tgaattcgga caaccggtgg tgtttttgca catgaggatt
     3781 tgaattgata aggaatcttt gattcgttac aatttcgttc ttaaaatttt ttaacgtttg
     3841 acaacggata gaaagactcc aatggctgaa acaatgaaaa aacaggcgga ttcgcctgat
     3901 ttggtgtacg gtttggaaga caggccgccg ttcggtaatg cgctcttgag cgcggttacc
     3961 catcttttgg cgatttttgt gccgatgatt acgcccgcgc tgattgtggg cggcgcgctg
     4021 gaactgccgg tggaaatgac ggcgtatctc gtgtcgatgg cgatggttgc gtcgggtgtc
     4081 ggcacttatt tgcaggttaa ccgcttcggg ccggtcggtt cggggatgct gtccatccag
     4141 tcggtgaatt tctcgttcgt taccgtcatg attgcgctcg gtgcggggat gaaggagggc
     4201 ggcttgacta aggatgcgat gatttcgacg ctcttgggcg tatcgtttgt cggcgcgttt
     4261 ttggtgtgtt tctcggcgtg gcttctgccg tatttgaaaa aagtgattac gccgacggtc
     4321 agcggtgtgg tcgtgatgct gattggcttg agtttggtac acgtcggcat tactgatttc
     4381 ggcggcggct tcggcgcgaa ggcggacggc acgttcggct cgatggaaaa cttggggctg
     4441 gcttcgctgg tgttgctgat tgtgttggtg ttcaactgca tgaaaaaccc gctgctgcgg
     4501 atgagcggca tcgcggtcgg gctgattgcc ggctacattg tcgcgctgtt tttgggtaag
     4561 gtggattttt ccgcactgca aaacctgccg ctggttacgc tgcccgtacc gtttaaatac
     4621 ggttttgctt tcgactggca cgcatttatt gtggcgggcg cgattttcct gttgagcgtg
     4681 tttgaggcgg tcggcgattt gacggcgacg gcaatggtgt ccgatcagcc gattgaaggc
     4741 gaggaataca ccaaacgctt gcgcggcggc gtgttggctg acggcttggt gtcggtgatt
     4801 gcgacggctt tgggttcgct gccgctgacg acgtttgcac aaaacaacgg cgtgattcag
     4861 atgaccggcg tggcttcgcg tcatgtgggc aaatatattg ccgtgatttt ggtgttgttg
     4921 ggtctgttcc ccgttgtcgg acgcgcgttt acgacgattc cgagtccggt gttgggcggc
     4981 gcgatggttt tgatgtttgg cttgattgcg attgcgggcg tgcggatttt ggtcagtcac
     5041 ggcatccgca ggcgcgaagc ggtaattgcg gcaacttcgg tcggtttggg tttgggtgtc
     5101 gcgtttgagc cggaagtgtt taaaaacctg cccgtcttgt tccaaaactc tatttctgca
     5161 ggcggcatta cggcagtctt gctgaatttg gtcttgcccg aagataaaac cgaggcggcg
     5221 gtcaagtttg ataccgacca cttggaacac tgattttgaa aatgaatgcc gtctgaaaca
     5281 gaatccctgt ttcagacggc attgtttttg aggcttacgc tttttcgttt ttcaatacgc
     5341 gttgccggcg ggtttcgctt aataccattc cggcagacac ggagacgttc atgctttcga
     5401 ccgtgccgaa catgggtatg gacaccagca tgtcgcagtg ttcgcgcgtg aggcggcgca
     5461 taccgtcgcc ttcgttgccc attacccacg ccgcgctgtc gggcaggacg caatggtaaa
     5521 ggtcggactc gccgctcata tcggtgccga taatccaaat gccgtattct ttcaattcgc
     5581 gcagggtgcg ggcgaggttg gttacggtga tataggggac ggtttccgcg gcaccgcagg
     5641 cgactttgct gacggtggcg ttcagccccg cgcttttgtc tttcggtgcg atgacggcgt
     5701 gtacgcccat tgcgtcggcg gtacgcaggc acgcgccgag gttgtgcgga tcggtgatgc
     5761 cgtcgagtat cagcagcagc ggcggttcgc ttaagttttc caatacgtct tcgagatgga
     5821 cgtggttttt ggaggcatcg ataaatccgg ccacaccctg atggcgcgcg cctttgctga
     5881 tggcatcaag acgggtggtg tcaacaaaat atacgcggat gttttcgttt gccgcctttt
     5941 ccaacacttc gcgcgtacgg gcatcgtttt tgccttcttg gatgtagagt tcggtaatcg
     6001 atttcgggtt ttgccacaat cgggcgttga cggcgtggaa gccgtaaata agtctttgat
     6061 ttgccataat ggtgctttgt gaaaagggtt cagacggcat tatagcaatt tgccggtatg
     6121 ccgtctgaag ggtttaaaac aggtaggcga tgtatttcac caacaggata aacaagatgg
     6181 atacggcgca gccgattttg aacgccgtac cgatgacaag tcccaacagt gtacccaagc
     6241 ccgctttacc tgcctgaagc atattgcgtc tgtcgatgag ttcgcctgcc gccgcgccga
     6301 taaaggggcc gagtattagt ccgggcaggg agaaaaacat accgatgatg ctgccgataa
     6361 aagcaccgcg cacggcgagc ctgcctgctc cggtatattt tacccccaat atgcctgcta
     6421 catagtccgc cagtatgccg gcaaggctga tgagtccgac cgtccacaaa acgcccgcgc
     6481 cgtagatttg gtatccgccg gcataggcaa gcagccatgt tccggcaaac atcaatgcca
     6541 atccgggcag ggcggggtaa acgatgcccg ccgtaccgac ggctatcagg gcgagggcga
     6601 ggatgacgag caaggcggtc ataggttcaa ccttttcttt tgttctgaaa aaaacggctt
     6661 aatacggcgc ggcattcttc ttgcaggatt ccgccccgta tggcggtgtg cgtattgagg
     6721 cgtttgtcgg caaacaggtt gacgatgctg cctgccgcgc cggttttggg ttctgccgca
     6781 ccgtagatca cgcgcctgat tcgtgcctgt atcagtgcgg acgcgcacat ggcgcagggc
     6841 tcgagggtga tgtagatgtc gcatccgtca aggcggtagt tttgcatttc gctgcctgcc
     6901 tgtgccaagg cgttgatttc ggcgtgtcgg ctgacattgc agtcggcaat gcaggtgttg
     6961 tgtgccgatg cgatgatttt gccgtctgaa acgatgattg cgccaacggg tatttcgccg
     7021 tcggcggcgg attgttctgc ttggcgcagt gcttcgcgca tgaagtgttc catttcttcc
     7081 tgcggcggaa aggcggcgac gggcggatgg tttttcaact cggcaagcag gcagtcttta
     7141 tgcgcttggg acatttcttg cggcggcgtg ccgttcagca gcgactcaag ctgccacagc
     7201 gtgcttttcg tgagggtcaa acccgatgcc ttcaataaca agaaggtttg aaccgcgccg
     7261 gtttgacgca gttcttcgag cgtacggata ccgagcctgt gtagggcggc gacggttttg
     7321 ggggcgagcg gcggcgtggt cagcatagtt tatacaccga aaaaccgttt tgccgcctca
     7381 atcagatgtg tgcctgaagt gccgtctgaa aacgggtcgg caacgcagtc taaaggtgtt
     7441 ttgcgcaacc aagtcagttg gcgtttggca agttggcgcg tggcggcaat gcctttctcg
     7501 acaaatgccg ggaaatcggt ttttccgtcc agatattccc atgcctgacg gtagccgacg
     7561 cagcgtatgg caggggagtc ggcggtcaga ccgggataga ggcggcgcag gttttctact
     7621 tcgccgataa agccctgttc aagcatcagg tggaaacgca gggcgatatt ttcatgcagg
     7681 cgggcgcggt tttcgggaat cagagcggtg gtatgtaaat caaaagggag cgtatgggag
     7741 gtcaggctgc cgagatgtgt gctcatcggt ttgccggtta aataataaac ttccaaagcg
     7801 cgtccgatac gctggctgtc gttcggtttc agacggcatg cggtttcagg gtcgactttt
     7861 tgcagggtgc ggtagaggaa atccaagccg tacatctgtt tttgttcgtc caagtcggca
     7921 cgcaggcagg cgtcggcttc gggcaaatcg ttcaaacctt gggtcagggc gcggaaatac
     7981 atcatcgtgc cgccgacaat aagggcaaac ctgccgcgtg aggaaatttc cccgaccaag
     8041 cgcgtgcagt cttcgacaaa gcgggcggcg ctgtatgatt cggtaggcgg gatgatgtcg
     8101 ataaggtggt gcgggacaaa ggcgcgttcg gaggcggacg gtttcgccgt gccgatgtcc
     8161 atatcgcggt aaaccagcgc ggaatcgagg ctgatgattt cgacaggcag ggtttcggca
     8221 attttgaggg cgagcgcggt tttgcctccg gcggtcggcc cgagcagggc aaaggctttc
     8281 ggggtcggca taacgtttca ggtttggaaa aatacggatt atagcggaaa gcgtgccgac
     8341 gttatatttt ggtttgcgga agcacgccga cggcaagggg gcgtgtttac cgtatgcctt
     8401 tataaaattt caaaccgacg cgccgggttt tcaatatgcc cgcgcccgat gcagccttgt
     8461 cctcaggcat cggcggcagc gtccgatttt tcggggaacg cccgtctcgg gcgtatttaa
     8521 aggttcggcg gtgcggtgtt ttcctgcggc aaggcttcag acggcatcgt cggcgcatcc
     8581 gttagacaag gcgcgtgctt ggggcgataa tggcgttttg ctttttttgt ttgaaagcct
     8641 tgcgatgttc cgaaacctgc ttgtccgctg gcttgccgtc tgcctgatac ccttggcgac
     8701 gcttgccgtt ttcgccgcca atccgcccga agacaaaccc cagcatctga ttaacggcat
     8761 catccttgcc tgcgaagcga cgtttctgtt caaattcgtc cttttcgaca ccatcaagca
     8821 tcatttgaaa caagagtttg atttgaaaca tcaaactatg tttctgttta ttccgattgt
     8881 tttgctggtt gtgtatttgt tccattattt tggcgcattt tagcttgttt tctagatggt
     8941 tatgaataca tacgcttttc ctgtctgttg gattttttgc aaggtcatcg acaatttcgg
     9001 cgacatcggc gtttcgtggc ggcttgcccg tgttttgcac cgcgaactcg gttggcaggt
     9061 gcatttgtgg acggacgatg tgtccgcctt gcgcgcgctt tgccctgatt tgcccgatgt
     9121 tccctgcgtt catcaggata ttcatgtccg cacttggcat tccgatgcgg cagatattga
     9181 taccgcgcct gttcccgatg ccgtcatcga aacttttgcc tgcgacctgc ccgaaaatgt
     9241 gctgcacatt atccgccgac acaagccgct ttggctgaat tgggaatatt tgagcgcgga
     9301 ggaaagcaat gaaaggctgc acctgatgcc ttcgccgcag gaaggtgttc aaaaatattt
     9361 ttggtttatg ggtttcagcg aaaaaagcgg cggactgata cgcgaacgcg attactgcga
     9421 agccgtccgt ttcgatagcg aagccctgcg tcagcggctg atgcttcccg aaaaaaacgc
     9481 ccccgaatgg ctgcttttcg gctatcggag cgatgtttgg gcaaagtggc tggaaatgtg
     9541 gcaacaggca ggcagcccga tgacactgtt gctggcgggg acgcaaatca tcgacagcct
     9601 caaacaaagc ggcgttattc cgcaagatgc cctgcaaaac gacggcgatg tttttcagac
     9661 ggcatccgtc cgcctcgtca aaatcccttt cgtgccgcaa caggacttcg acaaactgct
     9721 gcaccttgcc gactgcgccg tcatccgcgg cgaagacagt ttcgtgcgcg cccagcttgc
     9781 cggaaaaccc ttcttttggc acatctatcc gcaagacgag aatgtccatc tcgacaaact
     9841 ccacgccttt tgggataagg cacacggttt ctacacgccc gaaaccgcat cggcacaccg
     9901 ccgcctttca gacgacctca acggcggaga ggctttatcc gcaacacaac gcctcgaatg
     9961 ttggcaaatc ctgcaacaac atcaaaacgg ctggcggcaa ggcgcggagg attggagccg
    10021 ttatcttttc gggcagcctt ccgcatccga aaaactcgcc gcctttgttt caaagcatca
    10081 aaaaatacgc tagaatagcg cgttttacga caaccgattt gattggaaaa tcacaatgaa
    10141 aacagcacaa gaactgcgcg ccggcaatgt atttatggtc ggcaacgatc ctatggtcgt
    10201 tcaaaaaacc gaatacatca aaggcggccg ctcttccgcc aaagtcagca tgaaactgaa
    10261 aaacctgctg accggcgcgg cttccgaaac catttacaaa gccgacgaca aattcgacgt
    10321 ggtcatcctg tcccgcaaaa actgtacgta cagctacttt gccgacccga tgtacgtctt
    10381 tatggatgaa gaattcaacc aatacgaaat cgaagccgac aacatcggcg acgcgttgaa
    10441 attcatcgtt gacggtatgg aagaccaatg cgaagttacc ttctacgaag gcaaccccat
    10501 ttctgtcgaa ctgcccacca tcatcgtgcg cgaagtcgag tacaccgagc ctgccgtcaa
    10561 aggcgacact tccggcaaag tgatgaaaac cgcgcgcctg gtcggcggca ccgaaatcca
    10621 agtgatgtct tacatcgaaa acggcgacaa agtcgaaatc gatacccgca ccggcgaatt
    10681 ccgcaaacgc gcttgatttg ttgcattgaa aaatgccgtc tgaaaacgtt tcagacggca
    10741 tttttttatc ttcgccccaa gtttggattg aagtagatgt ttttttcgta aacgacaata
    10801 cgcgtgattt tgccattttc gtcaaaatgg atgtctaaaa acggtttgtc gggattgcgt
    10861 tcacggttgg acagccggaa gagtgattgg ttttcaagca tttctccgtg tattttcaga
    10921 tagccgggca attggccgat gtattggaaa ttgccgccaa gtccgttgtt gtgccggctc
    10981 gaataggcag gggttgccga aacttcaaaa taacgcgtgc gtttcggctc ctcgccttgt
    11041 ccgcgggttt cttgactgcc ctgtatcagc aacagcgtcg cttctcggag gcggcgttcc
    11101 tgttccatca aggcatcttg tgcctgccgg ctgatcgtgc gctttccgta acgcaagtct
    11161 tcaattgcca gtatgatttt ctgatggtag tcgggatctt cagggcggat gtcgggaaac
    11221 agcagccgct gtatgttgtc atagccgccg ccgggtaagc cgaaacgggt ttccagttcg
    11281 tgatgggaaa gggcaaacag acagtccgag tcgatgtgtt cggcgatttg ctgcatgaga
    11341 tcgtcaatgc cggtggcggg gtgcaggtcg gtgcaatttg acggcggcgc gctttctgcc
    11401 tgcttgacgt tatcaggggc ggatgcggcc ggttgcaaat cggattggga agaagctgat
    11461 gacgcggagg acgaagcagc agatgcaccg acttctttgg ttttatcgtt tttgttcgga
    11521 gaacacgcgg tcagcatgat tgcggtaagc cataagagaa gtgatgaggt tttgtttttc
    11581 attctattgt ttccagtatt aaagaggccg tctgaaaacc taccgtttca tttttcagac
    11641 ggcctgttgt taatagaacc gaagaacctg ttattgccga caaggttctc aacctgtctt
    11701 acccgacgcg gtaaaacgcc aggctgccca aaaggttggg gaaatgtttg acctgtctgt
    11761 tgctcgtcat cacggcgcgt tcgaggacgc ggatgttgtt tttggcgcac aacaaatcaa
    11821 aatctttgag cgtgcaccaa tggatattgg gcgtgtcgta ccaatggtag ggcatacgtt
    11881 cggaaaccgg catatgtccg ccgagtgcga tttggacgcg gttgcgccag tagccgaaat
    11941 tcgggaagct gacaatcgcc tgtttggcaa cgcgcatcag gcagcgcagg attttttcgg
    12001 tattctgcat cgcttggatg gtttggctca acacaatcac atcaaaactt tgatcgttga
    12061 atgcggttaa accttcttcc aaatcggctt ggataacatt tacgccgcgc gacatcgcgg
    12121 cgatgacgct gtcggtgtcg atttcgatgc cgtagccgct gcattttttg tgttcgacca
    12181 atgcggcaag cagttcgccg tcgccgcagc ccaagtccaa gacgcggctg ccttcgggta
    12241 tccggtcgta aatcagttgc aaatcatcgc gcaggttcat tgctgacatt ccttataaac
    12301 gttgttcata taggcggcga ccgcacgcat ataggcttcg tcttccatta aaaaggcatc
    12361 gtgcccgtgt gcggatttga cttcgatata ctgcacggat ttttgggcgg caatcagggc
    12421 cttgaccagt tcgtgcgaac gttcgggcgc gaaacgccaa tcggtgctga agctggcgac
    12481 aaagaatttt gcctgaacat cctgcacggc gcgggtcagg ctgttgccga aatccgccgc
    12541 cggatcgaaa tagtccaaag ccttggtcat cagcaaatag gtgttggcat caaaccgccc
    12601 gacgaatttg tcgccttgat agcgcagata ggattctact tcaaattcaa cgccatagcc
    12661 gtattgatag ccgttggaac gcaaatcgcg tccgaatttt ttgcccaaac cgtcttcggc
    12721 aagataggtg atgtgcccca tcatgcgggc aatccgcaag ccccgagcgg gaacggtgtt
    12781 gcggctgcgg taatgtcctt cgttgaaatc gggatcggtc aaaatcgcct gacgcgccac
    12841 atcgttgaac gcgatatttt gcgtggacag tttcggcgcg gacgcaatca ccaaggcgtg
    12901 gcgcacgcgt tcgggatagg aaatcgccca ctgcaaagcc tgcatgccgc ccaagctgcc
    12961 gccgacaacc gccgcccatt gcccgatgcc gagataatcg gcaagcgcgg cttgggattt
    13021 tacccagtcc ttcaccgtaa ccagcggaaa atccgcgccg tattccctgc ccgtttcagg
    13081 attaatcgac aaaggcccgc tgctgccgtc gcagccgccc agattattca aaccgaccac
    13141 gaaaaaacgt tccgtatcaa tcggtttgcc aggtccgacc atattgtccc accagcccgt
    13201 atatttatct tccgccgaat gcctgcccgc aacatgatgg ttgcccgaca gcgcgtggca
    13261 gattaaaacc gcattgtttt tttcagcatt cagctcgccg taggtttcaa tcatcagatc
    13321 gaaacgcggc aaagttttac cgttttccaa aaccagcggc atctcaaacg gaattttttg
    13381 gggcattaca atgcccaccg aggcattttg actcatatcc tgttccaaca aatgcggcga
    13441 aaagcgttat tatatcgcaa acggcatgac tttttgacac ggtcggacaa gcagccggac
    13501 gcgtttgacc ctcatccgcc gcacacgaat catacttttt cagacgacct ccaccgcttc
    13561 ccgacatgat aggcagactt ttccgtattt ttttcttttt cgcacttgcc gcgttgatta
    13621 tcaaccgcct tttcagccgc aggcaaaaac gcgccctgcg cgaagtcgcc gaaatcagcg
    13681 catgggtact gctcggtgca gccgccgcga tgctgttttg gtatctgttt atgctgtatt
    13741 tcaaacacat tccggattcg tattgacgga aaaaatgccg tctgaaacgc atttttctgt
    13801 ttcagacggc atatttgatg aaaagggctt gcggtaggag gtgctttata gtggattaaa
    13861 tttaaaccag tacggcgttg cctcgccttg ccgtactatt tgtactgtct gcggcttcgt
    13921 cgccttgtcc tgatttttgt taatccacta tacaaccgaa gcaggaaggg cagggagtca
    13981 gcgttggcgc gctttaaaac gcggattgct tttgcagatg acgtaaactt tgcccctgcg
    14041 tctgacgatt tggcagtcgc ggtggcgttg tttggcggtt ttgagtgaag acagaacctg
    14101 cattatttgt cctttctaaa cgatgacatg acggattgga aacgttggtt gaacttgctg
    14161 gcgcgtcctt cggtgttgac gttacgctgc ttgccggtat agacgggatg ggatgcggaa
    14221 gaagtatcca gcgaaaacag cggatattct ttgccgtctg tccaaaccat cgtttttccg
    14281 tgtgtttcgg cacaggagcg gattaaccag ccttcattgg cgctgctgtc gaaaaaaagg
    14341 acggttcgat aattgtcagg atgaatattt ggtttcatat attgccttgc tttcagtgtt
    14401 ataacataac aaactctagc atagtttaga agggctgtac aaggaaattt aactattttt
    14461 gtaatatatt agaaattttc atgataaatc tgaaaatttt gaaattgact catgtttggc
    14521 gcaactttat tatgttgcct gaaacatcat ataaaagata ataaaaggta cgcagccatg
    14581 aattacgcaa aagaaatcaa tgcgttaaat aacagtcttt ctgatttgaa aggcgacatt
    14641 aacgtttcgt ttgaattttt cccgccgaaa aacgaacaaa tggaaacgat gctgtgggac
    14701 tccatccacc gtctgcaaac cctgcatccc aagttcgtat ccgtaactta cggcgcaaat
    14761 tccggcgaac gcgaccgcac gcacggcatc gtcaaacgca tcaaacagga aaccggcttg
    14821 gaagccgcgc ctcacctgac cggtatcgac gcttctcccg acgaattgcg ccaaattgcc
    14881 aaagattatt gggacagcgg catccgccgc attgtcgccc tgcgcggaga cgagccggcc
    14941 ggttatgaga aaaaaccgtt ttacgccgaa gacttggtta agctattacg ctccgtcgcc
    15001 gacttcgaca tctctgtagc agcatatccc gaagtgcatc ccgaagcgaa atccgcacaa
    15061 gccgacctga ttaatttgaa acgcaaaatc gatgcgggcg cgaaccacgt catcacccaa
    15121 ttcttcttcg atgtggaacg ctacctgcgc ttccgcgacc gctgcgtgat gttgggtatc
    15181 gatgtggaaa tcgtccccgg tattttgcct gttaccaact tcaagcagct cggtaaaatg
    15241 gctcaagtaa ccaacgtcaa aatcccaagc tggctgtcgc aaatgtatga aggtttggac
    15301 gacgaccaag gtacgcgcaa tctggtggcg gcaagtatcg ccatcgatat ggtcaaagtc
    15361 ctgtcccgcg aaggcgtgaa agatttccac ttctatacgc ttaaccgcag cgagctgact
    15421 tacgccattt gccatatttt aggcgtgcgc ccttaaagcc gtatcaaaca gtttcagacg
    15481 gcatctaagg tgtctaaaaa gcaaaacacc gccccatccg agccattctg atttacaata
    15541 ccggccgatt cggattgaac cggtccttac aaaatccaac tggagagttc aacatgacaa
    15601 cattacattt ctcaggcttc ccgcgtgtcg gcgccttccg cgaattgaaa ttcgcacaag
    15661 aaaaatactg gcgcaaagaa atcagcgagc aagaattgct ggctgttgct aaagacttgc
    15721 gcgagaaaaa ctggaaacac caggtcgctg ccaacgccga tttcgttgcc gtaggcgatt
    15781 tcactttcta cgaccacatc ctcgacctgc aagtcgccac cggcgcgatt cccgcccgct
    15841 tcggcttcga cagccaaaac ctgtctttgg aacaattctt ccaactggcg cgcggtaaca
    15901 aagaccaatt cgctatcgaa atgaccaaat ggttcgacac caactaccac tacttggtgc
    15961 ctgaattcca cgccgatacc ggattcaaag ccaatgccaa acactatgtt caacaactgc
    16021 aagaagccca agccctcggt ctgaaagcca aaccgaccgt tgtaggtccg ttgactttcc
    16081 tgtgggtggg taaagaaaaa ggcgccgtcg aattcgaccg tctgagcctg ttgcctaaac
    16141 tgttgcctgt ttacgttgaa atcctgactg ctttggttga agccggtgcc gagtggattc
    16201 aaatcgacga gcctgctttg gctgtcgatt tgcctaaaga atgggtggaa gcctacaaag
    16261 acgtttacgc tactttgagc aaagtcaacg ccaaaatcct gttgagcact tacttcggtt
    16321 ctgttgccga acacgccgca ttgttgaaag ccctgcctgt tgacggtctg cacatcgact
    16381 tggtacgcgc ccccgagcaa ctggacgcgt tcgccgacta cgacaaagtc ctgtctgccg
    16441 gcgtgattga cggccgcaac atttggcgcg ccaacctgaa caaagttttg gaaactgtcg
    16501 agcctctgca agccaaactg ggtgaccgtt tgtggatttc cagctcttgc tcgctgctgc
    16561 acactccatt tgacttgtca gttgaagaaa aactgaaagc caacaaaccc gacctgtact
    16621 cttggttggc attcaccctg caaaaaaacc aagaattgcg cgttctgaaa gctgcattga
    16681 acgaaggccg tgattctgtt gccgaagaac tcgccgccag ccaagctgct gccgactccc
    16741 gtgccaacag cagcgaaatc catcgtgcag acgttgccaa acgcctggcc gatttgcctg
    16801 ccaacgcaga ccaacgcaaa tctccatttg ccgaccgtat caaagcgcaa caagcatggt
    16861 tgaacctacc tctgctgccg acgaccaaca tcggttcttt cccgcaaacc accgaaatcc
    16921 gccaggcacg ctcagccttc aaaaaaggcg aactgtctgc cgccgattac gaagccgcga
    16981 tgaaaaaaga aatcgccttg gtggttgaag agcaagaaaa actggacttg gacgtactgg
    17041 tacacggcga agccgagcgt aacgacatgg ttgaatactt cggcgaattg ttgagcggtt
    17101 ttgcattcac tcaatacggc tgggtacaaa gctacggctc acgctgcgtg aaaccaccga
    17161 ttatctttgg cgacgtaagc cgtcctgaag ccatgaccgt ggcttggtct acttacgctc
    17221 aaagcctgac caaacgcccg atgaaaggta tgttgaccgg ccctgtaacc attctgcaat
    17281 ggtctttcgt ccgcaacgat attccccgcg ctaccgtgtg caaacaaatc gcactggctc
    17341 tgaacgacga agtattggat ctggaaaaag caggcatcaa agtcatccaa atcgacgaac
    17401 ctgccatccg cgaaggtctg cctttgaaac gtgccgattg ggatgcctac ctgaactggg
    17461 cgggcgaatc tttccgcctg tcctctgccg gttgtgaaga cagcactcag atccacaccc
    17521 atatgtgtta ctccgagttc aacgacatct tgccggccat cgccgctatg gatgctgacg
    17581 tcatcaccat cgaaacttca cgttccgata tggaactctt gaccgcgttc ggcgaattca
    17641 aatacccgaa cgacatcggc ccgggggttt acgacatcca cagcccgcgc gtaccgactg
    17701 aagccgaagt tgagcacctg ttgcgcaaag ccatcgaggt tgtaccggtt gagcgtctgt
    17761 gggtgaatcc tgactgcggc ctgaaaacac gtggctggaa agaaactctg gaacaactcc
    17821 aagtgatgat gaacgtaacc cgaaaactgc gtgccgaatt ggcgaaataa gccgagaccg
    17881 tatgaataaa taccgtctga aagcctttca gacggtattt tgtcctgatt tgcggcgcaa
    17941 gggcgcagtt gccggaaaat cttttcattg cagcttgttt ttttctcatt cggctttata
    18001 tgtgggaaac aggcaaatcg gagttgtgtt tgatagtttt aaataattta tattatttga
    18061 actataaatt atacaaatca ttttgcatgg ggtagaatgc ccagcgattc acaattattt
    18121 ctcaaaccaa tctattaagg agcttaaaat ggctttgcaa gatcgtaccg gtcaaaaagt
    18181 accttccgta gtattccgca cccgcgtcgg cgacacttgg aaagatgtgt ctaccgatga
    18241 tttgttcaaa ggcaaaaaag tagtcgtatt ctccctgccc ggtgcattta ccccgacttg
    18301 ttcttcttca cacctgccgc gttacaacga attgttcggc gcgttcaaag aaaacggcgt
    18361 tgacgcaatc tactgcgtat ctgtaaacga tacgttcgta atgaacgctt gggctgccga
    18421 agaagaatcc gacaacatct acatgattcc tgatggcaac ggcgaattta ccgaaggtat
    18481 gggtatgctg gtcggtaaag aagacttggg cttcggtaaa cgctcttggc gttactccat
    18541 gctggttaac gacggcgtgg ttgaaaaaat gttcatcgaa cctgaagaac cgggcgatcc
    18601 gttcaaagta tccgatgcag atactatgct gcaattcgtt gctcccgatt ggaaggctca
    18661 agagtctgtg gcaattttca ctaaaccagg ttgccaattc tgcgctaaag ccaaacaagc
    18721 tttgcaagac aaaggtttgt cttacgaaga aatcgtattg ggcaaagatg caaccgttac
    18781 ttccgttcgc gccattaccg gcaagatgac tgcccctcaa gtcttcatcg gcggcaaata
    18841 catcggcggc agcgaagatt tggaagctta cttggctaaa aactgatagc tgtttgctta
    18901 aggcggtttg attaaactgt ctgatatacc ggatagagtt attcgggcgg ttctgcactg
    18961 ctgctccgaa taactctata tttataagag aatttggata ttgttgcact caatcgaaat
    19021 tttgttttta tttatctgaa tgatgttttt gattgggaaa atatttaaat gccgtctgaa
    19081 accgatatgt tctgtgtcgg caatgtttca gacgaaaacg gaaggacaaa gattatgaaa
    19141 aaaattcaag cggatgtcgt cgtactcggc ggcggtactg ccggtatggg tgcgtttcgc
    19201 aatgcccgtt tacattcgga taatgtttac ctgattgaaa acaatgtgtt cggcacaacc
    19261 tgcgcgcgcg tgggttgtat gccttccaaa ctcttgattg ccgccgcaga agcgcgccac
    19321 cacgcattgc ataccgactc gttcggcgtg catttggaca aagacagcat cgtcgtcaac
    19381 ggtgaagagg tcatgcagcg cgttaaatcc gagcgtgacc gttttgtcgg ctttgtcgtt
    19441 gccgatgtgg aagagtggcc tgccgacaag cgcatcatgg gttcggctaa atttatcgac
    19501 gagcataccg tccaaatcga cgagcatact caaattacgg caaaaagttt cgtgattgct
    19561 accggttcgc gtcccgtcat cctgccgcaa tggcagtctt tgggcaatcg tttgattatc
    19621 aacgatgacg ttttctcatg ggatacgctg cctaagcgcg ttgccgtgtt cgggccgggt
    19681 gttatcggtt tggaactggg tcaggcattg caccgtttgg gcgtgaaagt tgaaattttc
    19741 ggtttgggcg gaatcatcgg cggcatttcc gaccccgtcg tttcagacga ggcgaaagcc
    19801 gtgttcggcg aagaattgaa actgcatttg gatgctaaaa ccgaggtcaa gctcgatgca
    19861 gacggcaatg tagaagtcca ttgggagcag gatggcgaaa aaggcgtatt tgttgccgaa
    19921 tatatgctgg cagccgtggg ccgccgtccg aacgttgaca atatcggttt ggaaaatatc
    19981 aatatcgaaa aagatgcgcg cggcgtacct gttgctgatc cgctgaccat gcagaccagt
    20041 attccgcata tcttcatcgc aggcgatgcg tccaaccaac tgcctctgct gcatgaagct
    20101 gccgaccaag gcaagattgc cggcgataac gcgagccgct acccgaatat cggcggcggt
    20161 ttgcgccgca gcaccatcgg cgtggtgttt accagtccgc aaatcggctt tgtcggtctg
    20221 aaatacgcgc aggttgccgc gcaataccaa gccgacgaat ttgtcatcgg cgaagtatcg
    20281 ttcaaaaacc aaggccgcag ccgcgtgatg ctggtaaaca aaggccatat gcgcctgtat
    20341 gccgaaaaag ccaccggccg ctttatcggc gcggaaatcg taggccctgc cgccgaacat
    20401 ttggcgcact tgttggcatg ggcgcatcaa atgaagatga ccgttccgca aatgctggat
    20461 atgccgttct accatcccgt tatcgaggaa ggtctgcgta ccgcgttgcg cgatgccgat
    20521 gcgaaattga aagcctgacc gatatggcaa aacaatgccg tctgaaattt tttcagacgg
    20581 cattttgttt ttggggatgg ggtcggatgc tgataccgtg tcgggaaggg ggcggcaaaa
    20641 ctaaaaatct ttcttttaat ctgctgtttc cacgcgtgtt tgtcaaaatc tatcagtttg
    20701 tttttaaaat acactgttca aaatgggata aaacaggtaa attaacgttt atgtaaccca
    20761 gtgtagcaat gggtttacgg tttttgagtc gatatataac tacagaggaa ttgactatgt
    20821 ctgccaaacc gcgtcctgtt tatctggatt tgccgaacat ccgtctgccg atacccggga
    20881 tagtttccat ccttcaccgc atcagtgggg tcgggctgtt tattatgctg cctttcctgc
    20941 tgtatttcct gtccggtacc ctgagtcaag agtctgcatt tgaaacttac cgtgccattg
    21001 tttcccatcc tttggtcaag ctggttttaa tcggtgtatt gtgggcttat ctgcaccatt
    21061 ctctcgccgg tatccgcttt ttatttttgg atgcgcacaa aggccttgag ctgaatactg
    21121 cgcgcaatac cgctaaagcc gtatttgctt ctgcattggt tttgactgtc gttttgggag
    21181 cgttgttatg gtagaacgta aattgaccgg tgcccattac ggtttgcgcg attgggcgat
    21241 gcaacgtgcg actgcggtta ttatgttgat ttataccgtt gcacttttag tggttctatt
    21301 ttccctgcct aaagaatatt cggcatggca ggcatttttt agtcaaactt gggtaaaagt
    21361 atttacccaa gtgagcttca tcgccgtatt cttgcacgct tgggtgggta tccgcgattt
    21421 gtggatggac tatatcaaac ccttcggcgt gcgtttgttt ttgcaggttg ccaccatcgt
    21481 ctggctggtc ggctgcttgg tgtattcaat taaagtaatt tgggggtaag tatgggtttt
    21541 cctgttcgca agtttgatgc cgtgattgtc ggcggtggtg gtgcaggttt acgcgcagcc
    21601 ctccaattat ccaaatccgg tctgaattgt gccgttttgt ctaaagtgtt cccgacccgt
    21661 tcgcataccg tagcggcgca gggcggtatt tccgcctctt tgggtaatgt gcaggaggac
    21721 cgttgggact ggcacatgta cgataccgtg aaaggttccg actggctggg cgaccaagat
    21781 gcgattgagt ttatgtgccg cgctgcgcct gaagcggtga ttgagttgga acacatgggt
    21841 atgccttttg accgtgtgga aagcggtaaa atttatcagc gtcctttcgg cggccatact
    21901 gccgaacacg gtaaacgcgc ggtagaacgc gcctgtgcgg ttgccgaccg tacaggtcat
    21961 gcgatgctgc atactttgta ccaacaaaac gtccgtgcca atacgcaatt ctttgtggaa
    22021 tggacggcac aagatttgat tcgtgatgaa aacggcgatg tcgtcggcgt aaccgccatg
    22081 gaaatggaaa ccggcgaagt ttatattttc cacgctaaag ctgtgatgtt tgctaccggc
    22141 ggcggcggcc gtatttatgc ctcttctacc aatgcctata tgaataccgg cgatggtttg
    22201 ggtatttgcg cgcgtgcagg tatcccgttg gaagacatgg aattctggca attccacccg
    22261 accggcgtgg caggtgcggg cgtgttgatt accgaaggcg tacgcggcga gggcggtatt
    22321 ctgttgaatg ccgacggcga acgctttatg gaacgctatg cgccgaccgt aaaagacttg
    22381 gcttctcgcg acgttgtttc ccgcgcgatg gcgatggaaa tctacgaagg tcgcggctgc
    22441 ggtaaaaaca aagaccatgt cttactgaaa atcgaccata tcggcgcaga aaaaattatg
    22501 gaaaaactgc cgggcatccg cgagatttcc attcagttcg ccggtatcga tccgattaaa
    22561 gacccgattc ccgttgtgcc gactacccac tatatgatgg gcggtattcc gaccaactac
    22621 catggcgaag ttgtcgttcc tcaaggcgac gaatacgaag tgcctgtaaa aggtctgtat
    22681 gcggcaggtg agtgcgcttg tgcttccgta cacggtgcga accgcttggg taccaactcc
    22741 ctgttggact tggtggtatt cggtaaagct gccggcgaca gcatgattaa attcatcaaa
    22801 gagcaaagcg actggaaacc tttgcctgcg gatgcgggcg agctgacccg ccaacgtatc
    22861 gagcgtttgg acaatcaaac tgatggcgaa aacgttgatg cattgcgtcg cgaattgcaa
    22921 cgctccgtac aactgcacgc cggcgtgttc cgtactgatg agattctgag caaaggcgtt
    22981 cgagaagtca tggcgattgc cgagcgtgtg aaacgtaccg aaatcaaaga caagagcaaa
    23041 gtgtggaata ccgcgcgtat cgaggctttg gaattggata acctaattga agtggcgaaa
    23101 gcgactttgg tgtctgccga agcacgtaaa gaatcacgcg gtgcgcacgc ttcagacgac
    23161 catcctgagc gcgatgatga aaactggatg aaacatacgc tgtaccattc agataccaat
    23221 actttgtctt acaaaccggt acataccaag cctttgagcg tggaatacat caaaccggcc
    23281 aagcgcgttt attgatgcgt tttcagacgg ttttcgcctc ataagctcgt ctgaaatcta
    23341 accataccca cattgaactg cttgaattta taatacaaaa tcattgggca gttgatgaga
    23401 aaaggaacac ttctcatgga aaaaatgagt tttgaaattt accgttacaa cccggatgtt
    23461 gatgccaagc cttatatgca gcgttacgag ttggaattgg aaccgaccga cgtgaaactt
    23521 ttggatgctt tggtacgcct gaaagcacaa gacgatacct tgtctttccg ccgctcctgc
    23581 cgcgaaggca tttgcggttc tgacggtatg aacatcaacg gcaaaaacgg cttggcgtgt
    23641 ttgaccgatc tgcgtggctt gaaacagccg gttaaaattc gtcctctgcc aggtctgcct
    23701 gttatccgtg acctgattgt ggatatgacc cagttcttca aacaatacca ttccgtcaaa
    23761 ccttatgttg tcaacgataa tccgattgat gcggacaaag agcgtctgca aactcaggaa
    23821 gagcgtaaag agttggacgg tttgtacgag tgtattttgt gcgcctgctg ttcgactgcc
    23881 tgcccgtcat tttggtggaa ccctgataaa ttcgttggtc cgtccggttt gctgaatgct
    23941 taccgcttca ttgcggacag ccgagatacc atcactagtg agcgtttgga taatctgaac
    24001 gacccatacc gtttgttccg ttgccacacc attatgaact gcgtagacgt atgtcccaaa
    24061 cacttgaatc cgacccgagc catcggtaag attaaagaga ttatgttgaa acgggccgtt
    24121 taagaaatga tggtttttga cgatattgcc aaacggaaaa tccgttttca aacccgccgg
    24181 ggattgttgg agttagattt aatcttcggc aggtttatgg aaaaagaatt cgagcatttg
    24241 agcgataaag agctgtccga gttttccgaa atccttgaat ttcaagatca agaattgctt
    24301 gccttgatta acgggcattc ggaaacggac aaagggcacc ttatcccgat gcttgaaaaa
    24361 atcagacggg catgaggcat ctgaaatctg cctgcaggca gatttcaaaa tgcaaaagcc
    24421 gtctgaaggc aaagaacgtg ctgcggatgc agtaacgtgg gttataactt gcaaaggagc
    24481 aataatatgt ccaaatcaat caaactcaac gtaccgggtc aggcaggttt ggagctgccg
    24541 gtattggaag ccagcatcgg gcacgatgtg gttgacattc gggggctgac aaaaaataca
    24601 ggtttgtttt ccttcgaccc cggatttgtt tcaaccgcaa gctgtgagtc taaaattact
    24661 tacatcgacg gcgatcaagg cttgctttat tatcgcggat accccatcga gcagctggcc
    24721 gaaaagtccg attatttgga agtctgctac ctgttgattt acggcgaact gccgactccc
    24781 gagcaaaagg cagaatttga caataccgtc cgccgccaca cgatggtgca tgaacagctg
    24841 acttggttct tccggggctt ccgccgcgac gcgcatccga tggcgatgat ggtcggcgtg
    24901 gtcggcgcac tgtctgcgtt ctaccaagac agcttggaca ttagcaatcc cgaacaccgc
    24961 aaaatcgcga tttaccgcct gatttctaaa atcccgacca ttgcggcaat gtgctaccgc
    25021 tattcaaacg gtctgccgtt caattatccg aagaataatc tttcttattc cgaaaacttc
    25081 cttcatatga tgttcgccac gccgtgtgaa gactacaaac ccaatcccgt tttggcacgc
    25141 gcgctcgacc gcatctttat tttgcatgcc gaccacgagc aaaacgcctc aacttcaacc
    25201 gtccgtctgg caggctcttc gggtgcgaat ccgtttgcct gtattgctgc cggtatcgcc
    25261 tgcctgtggg gtgcttcgca cggcggtgcg aacgaagccg tgttgaaaat gttggacgaa
    25321 atcggcgatg tgtctaatgt tgccgcatac atggaaggtg tgaaacaacg caaataccgt
    25381 ctgatgggct tcggtcaccg cgtgtaccgc aatatggatc cgcgtgccag cattatgcgc
    25441 gaaacctgct atgaagtttt gaaggaattg ggcttggaag acagtccgaa attcaaactg
    25501 gcgatggaat tggaacagat tgcgctgaaa gacccgttct ttatcgaacg caaactgtat
    25561 ccaaacgtcg atttctattc cggcatcgtc ctgtccgcgc tgggcatccc gaccgaaatg
    25621 tttaccgtca tcttcgccct gtcgcgcagc gtgggctgga tttcgcactg gcacgagatg
    25681 attagcgatc cttcgctgaa aatcggccgc ccgcgccagc tttataccgg ttcggaacgc
    25741 cgcgattatg tgccgccagg cgagaggtaa catacattaa tatattgtca aacaggcaat
    25801 atcagagaac cggattgttt cccgaatccg tctgattgta gtcggatgaa atcaagacaa
    25861 gcaatccggt ttaaaatagg gtagaataaa atgtcttttc agacggcatc agtttagccg
    25921 tcaggacgcg gacttctacc ctttgtttat attttaaaga aaagagcgca cgccatgatg
    25981 gacgaaaaac tcaatttctc ttacctgttc ggttcgaacg caccctacat tgaggaattg
    26041 tacgaggctt ttttggaaaa ccccgatacg gttgatgaaa aatggaagca gtatttcacc
    26101 gatttgagca aacagccggg gacggttgct gtcgatgtcg cacacacacc gattcgcgaa
    26161 tcatttgtta ctttggcgaa aaagaaaatt gcatctgccg ttgcgggcgg tgcggatgag
    26221 gcaatgctga aaaagcaagt cagcgtttta cggctgattt ctgcctatcg tatccaaggc
    26281 gtgggtgcag cccaacttga tccgctcaaa cgtatccccc cgcgcgatat tgaagccctc
    26341 gatccgaaat tccacggtct gtctgatgcc gatatggcgc ttcaattcaa tatgggcgag
    26401 ggcgattttg ccaatcgcgg caaactgcct ttgtcccaaa tcatcagcaa cctcaaacaa
    26461 acctactgcg gccacatcgc attggaatat atctatattc ccaataccga agagcgccgc
    26521 tgggtacgca attattttga aagcgtattg tccacaccgc attacaatgc cgatcaaaaa
    26581 cgccgtatct tgaaagagat gactgctgcc gagactttgg aacgttatct gcataccaaa
    26641 tatgtcggtc agaaacgttt cggtgtcgaa ggcggcgaaa gcgcgattgc cggtttgaac
    26701 tacctgattc aaaacgccgg taaagacggc gtggaagaag tcatcatcgg tatggcgcac
    26761 cgtggccgtc tgaatgtttt ggtgaacatt ttgggcaaaa aacccggcga tttgtttgcc
    26821 gaatttgaag gtcgtgccga aatcaaactg cccagcggcg acgtgaaata ccatatgggc
    26881 ttcagctccg atattgctac cccgcacggc ccgatgcatg tttctttggc gttcaacccg
    26941 tcccacttgg aaatcgtcaa tccggtagtg gaaggttctg cacgcgccaa gcaaaaacgt
    27001 ttgggcgaaa acggccgcga taaagtcttg cccgtattga ttcacggcga ctccgcattt
    27061 atcggtttgg gtgtcaacca agcgacattc aacctgtcta aaacgcgcgg ttataccacc
    27121 ggcggtacgg ttcatatcgt catcaacaat caaatcggct ttaccacttc cgatatccgc
    27181 gatacccgtt caaccgtaca ctgtaccgat atcgcaaaaa tggtttccgc tccggttatc
    27241 catgtgaacg gcgatgatcc tgagcgtgtt tgctttgcca tccaagccgc tttggattac
    27301 cgcaaaaaat tccataaaga catcgtgatt gacgttgtct gctaccgtaa atggggtcac
    27361 aacgagggcg atgatccgac cttgacccaa ccgatgatgt acaaaaaagt atcgcaacac
    27421 cccggtgcgc gtgctttgta caccgagcaa ctgattgccg aaggcgtggt aacccaagcc
    27481 gaggcagacg gttacatcca agcctaccgt gatgctttgg acaaaggcga acacgtcgag
    27541 caaacaacgt tgagcaactt tcaacgcacg caaatcgact ggagcaaata ccaaggcaaa
    27601 gattggcgcg aacacatcga aaccggtttg cctgccgcag atattgagcg tctcactgag
    27661 aagtttaccg ccgtaccgga aggctttgcc ctgcatccga ctgcaaaacg tgtgattgaa
    27721 gcgcgtaaag ccatggcatc cggcaaacag gccatagatt ggggtatggc cgaaaccttg
    27781 gcatacgcca gcctcgtaac caaaggtcat ggcgtgcgta tctccggtga ggactccggc
    27841 cgcggtacgt tctcgcaccg ccacgccgta ttgcacgatc aaaaacgcga aaaatgggac
    27901 gacggtactt atgttcccct gcgccatatg ggcgaaggaa tgggcgagtt cctggttatc
    27961 gactccatct tgaacgaaga ggccgtgatg gcgttcgagt acggctttgc ctgctccgct
    28021 cctgacaagc tcaccatttg ggaagctcaa ttcggtgact tcgccaacgg cgcgcaagtg
    28081 actattgacc aattcctgtc ttcaggcgaa accaaatggg gtcgcttgtg cggtctgacc
    28141 accatcctgc cgcacggtta cgacggtcaa ggccctgagc actcttctgc acgcgtagaa
    28201 cgctggttgc aactgtgttc tgagaacaat atgcaagtca tcatgccatc tgaagcgtcg
    28261 caaatgttcc acctcttgca acgtcaagtc ttaggttcat accgcaaacc gctggtaatt
    28321 ttcatgtcca aacgcctgtt gcgattcaaa ggggcaatga gcccgctgga aaacttcacc
    28381 gaaggttcga ctttccgtcc ggttgtcggc gataccgccg aacgcgcaag caacgacagc
    28441 gtgaaacgcg tggtattgtg tgccggtcag gtttactatg acttggaagc cggtcgagcc
    28501 gaacgtaaac tggaagatga tgttgcgatt gtccgcgttg agcagctgta tccgttccca
    28561 tacgatgagg ttaaagccga actggcgaaa tatccgaacg caaaatctgt ggtttgggcg
    28621 caagaagagc cgaaaaacca aggcgcgttc taccaaatcc gccaccgcat cgaagacgtt
    28681 atcagcgaag agcaaaaact gtcttatgcc ggtcgtccaa gcagcgcatc gcctgcagtg
    28741 ggctactcaa gcaaacacat tgctcaattg aaacaattgg ttgaagacgc tttggcgttg
    28801 taaaccaagc agcattccgt ctgaggctgc tcaggcggaa tgcccatatg cagaatgaaa
    28861 aacacacaac aggccgtctg aaagggccat tggagacaca aaatgattat tgatgtaaaa
    28921 gtacctatgc tgtctgaaag cgtatctgaa ggcacgctct tggaatggaa gaaaaaagtt
    28981 ggcgaagcag ttgcccgtga cgaaatcctg atcgatatcg aaacggacaa agtggttttg
    29041 gaagtacctt ctccacaagc cggcgtattg gttgaaatcg tagcgcaaga cggtgaaacc
    29101 gttgttgccg accaagtttt ggcacgtatc gataccgctg ctactgccgc tgctgaagca
    29161 cctgccgccg ctcctgccga agctgccccg gccgccgctc ctgctgctgc acaaaacaac
    29221 gccgctatgc ctgccgccgc caaactggct gcagagagcg gtgttgacgt gaacgcattg
    29281 caaggttccg gccgtgacgg tcgcgtattg aaagaagacg tacaaaatgc cgcagccaaa
    29341 cctgccgcag ccgctgctcc tgccgttgca cttcctgccg gcgcacgtcc tgaagaacgc
    29401 gtaccgatga gccgcctgcg tgcccgtgtt gcagaacgcc tcttggcttc tcaacaagaa
    29461 aacgccattc tgactacatt caacgaagtc aacatgaaac caatcatgga cttgcgtgcg
    29521 aagtacaaag aaaaattcga gaaagaacac ggcgtgaaac tgggctttat gtccttcttc
    29581 gttaaagccg ctgttgcagc cctgaaaaaa tacccggttg tgaatgcttc tgttgacggc
    29641 aaagacattg tgtaccacgg ctacttcgac atcggtatcg cgattggcag cccacgcggt
    29701 ttggttgtgc caatcctgcg cgatgccgac caaatgagca ttgccgacat cgaacaagca
    29761 attgttgatt acgcgaaaaa agccaaagac ggcaaaatcg ctatcgaaga tctgaccggc
    29821 ggtacattca gtattaccaa cggcggtact ttcggttcta tgatgtccac cccgatcatc
    29881 aatccgcctc aatctgcgat tttgggtatg cacgccacta aagagcgcgc tgtggttgaa
    29941 aacggccaag ttgttgtccg tccaatgatg tatctggctc tgtcttacga ccaccgtatc
    30001 attgacggcc gcgaagctgt attgaccttg gtagccatta aagacgcgtt ggaagaccca
    30061 gcccgcctgt tgttggatct gtaatcgttt cagacggcct tttatttgtt aatgaaaagg
    30121 ccgtctgaat ttttatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg
    30181 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata
    30241 tttagatgta gcgtaatgta gtatcgtgct acaataggct caacaaacga ttgagaccgt
    30301 ctgaaacatt tgattcgaat gaatcggcag atatggactt tcagacggct ttttcttaaa
    30361 accatcaaaa cgcagtcatt caaaataaaa aagaaacgaa aagtatcgtt tttattttga
    30421 gatactgcta aaagcaaagg atgacacgat gtctcaatat gatgtagtag tgattggtgc
    30481 aggcccgggc ggatacgttg ccgccatccg tgccgcacaa ctgggtttca aaactgcctg
    30541 tgtcgatgca ggcgttaaca aagcaggcaa tgcccctgca ttgggcggta cttgcttgaa
    30601 cgtaggttgt atcccttcta aagccctgtt gcaatccagc gaacatttcc acgcagcgca
    30661 acacgagttt gccgaacacg gtatcactgt cggcgacgta aaattcgacg tggccaaaat
    30721 gattgagcgc aaagatgcca tcgtaaccaa actgaccggc ggcgtgaaat tcctgttcca
    30781 gaaaaacaaa gtaaccagcc tgttcggtac ggcttccttt gccggcaaaa atggcgatgc
    30841 ttaccaaatc gaagtcgata acaaaggtga gaaaaccgtt atcgaagcca aacacgtcat
    30901 cgtagcgacc ggttccgtac cgcgtccgct gccacaagtc gctatcgaca atgtgaacgt
    30961 attggacaac gaaggcgcat tgaacctgac cgaagtacct gccaaactcg gcgtgatcgg
    31021 ttccggtgtg attggtttgg aaatgggttc cgtatggaac cgcgtgggtg cggaagttac
    31081 cattcttgaa gccgcgccga ccttcctggc tgccgccgac caacaaatcg ccaaagaagc
    31141 cttcaaatac ttcaccaaag agcaaggtct gagcatcgaa ttgggcgtga aaatcggcga
    31201 catcaagtct gaaggcaaag gtgtttccgt tgcttacgaa actgctgccg gcgaagccaa
    31261 aaccgaagta ttcgacaaac tgatcgttgc catcggccgt attccaaaca ccaaaggcct
    31321 gaacgccgaa gccgtaggct tggaaaaaga cgagcgcggc tttatcaaag tagatggcga
    31381 atgccgtacc aacctgccta acgtatgggc aatcggcgac gtggttcgcg gcccgatgtt
    31441 ggcacacaaa gccagcgacg aaggcgttgc cgttgccgaa cgcattgccg gtcaaaaacc
    31501 gcatatcgac ttcaacaacg taccgttcgt gatttacacc gatcctgaaa tcgcttgggt
    31561 gggtaaaacc gaagagcagc tcaaagccga aggcgtggag tacaaaaaag gtacttcagg
    31621 ttttggtgcg aatggtcgcg cattggcaat gggcaaagcc aaaggtacgg ttaaagtgtt
    31681 ggcagacgcc aaaaccgacc gcatcttggg cgtacacatg attggtccgg ttgtcagcga
    31741 attgcttacc gaaggcgtga ctgcgctcga attcttcgcc agcagcgaag acatcgcccg
    31801 cattatccat gcccacccaa ccttgtccga agtggttcac gaagctgcat tggcggccga
    31861 caaacgcgct ttgcacggtt gatagacatt aaggcctccg acaaattgaa tgttccgaga
    31921 gcttcgtttt ctgatttata attccgtcag acaaacaaac agcatttaca ttcgttatga
    31981 acaaagaaat agtcggtatt ttctttatac cgatgggcat catcagcatg tgtatggccg
    32041 cattgtggca gatgtatgtg atgatgaccg aaacttatac gctcaaccgt ttcaaagata
    32101 aagaattggt ttggcgcgtg gcattgttgt ttatcagttt cagccttgcc gtttatctgc
    32161 tctgtccgaa ttcgcgtaaa aaaggcattg tcttttttat tctcggggga ggcggtgcag
    32221 ccatgtatct gctggcgcgg atgtggttgc ctttcagcaa gtgaaacgac gattttccga
    32281 ccgccgaaag gtagtctgaa acgcacgggc ttgccatttg gaggcaaact cggggcatta
    32341 cactaatcta aaggagaaat ccatgaattt acacgagtat caggctaaag aactgctggc
    32401 tggctacggt ttgcccgtac aaggcggtat tttggcacac aacggcgaag aagccgctgc
    32461 agcttacgac aaattgggcg gcaaattcgc tgttgtcaaa gcacaagtac acgccggcgg
    32521 ccgcggtaaa gcgggcggcg taaaagtcgt taaaagccgc gaagaagcta aagaagtggc
    32581 tgaaagcctg attggcacca acttggtaac ttaccaaacc gatgccaacg gtcaacctgt
    32641 caacagcgtt ttggtttgcg aagatatgta tccggttcaa accgagctgt acttgggcgc
    32701 agtggttgac cgttctaccc gccgcgttac attcatggct tctaccgaag gcggtgtgga
    32761 aatcgaaaaa gttgctgccg aaactccaga aaaaatcttc aaagtaaccg ttgatccgct
    32821 ggtcggcctg caaccttgcc aagctcgcga agttgccttc caactgggct tgaaagacaa
    32881 acaaatcaac gagttcgtca aactgatgac cggcgcgtac aaagcgtttg tcgaaaacga
    32941 cttcgccctg tttgaagtca acccgctggc agttcgcgaa aacggcgcac tggcttgcgt
    33001 ggacggcaaa atcggcatcg acagcaacgc gctctaccgc ctgccgaaaa tcgccgaatt
    33061 gcgcgacaaa tctcaagaaa acgaacgcga gctgaaagct tccgaattcg acctgaacta
    33121 tgttgccctg gaaggtaata tcggctgtat ggttaacggt gccggtttgg cgatggccac
    33181 catggacatc atcaaactga aaggcggcca acctgccaac ttcttggacg ttggcggcgg
    33241 cgcaaccaaa gaccgcgtgg ttgaagcgtt caaactgatt ctggaagaca aatccgttca
    33301 aggcgtattg atcaacatct tcggcggtat cgtacgttgc gacatgattg cggaagctat
    33361 cgtggcagcc gttaaagaaa tcaacgtcga cgttcctgtc gttgttcgtt tggaaggcaa
    33421 caacgccgaa ctcggcgcga aaatcctgaa cgaatcaggc ctgaaactga cttctgcaga
    33481 cggcctgaat gacgcagccg aaaaaattgt tgcagccgta aacgcctaag gagaaaagaa
    33541 tgagcgtatt gattaataaa gacaccaaag tattggttca aggtttcacc ggtaaaaacg
    33601 gtactttcca ctccgaacaa gctctggctt acggcactaa agttgtcggc ggcgttaccc
    33661 cgggcaaagg cggtcaaacc cacctgaacc tgcctgtatt caacaccatg aaagaagctg
    33721 ttaaagaaac cggcgcggat gcatccgtaa tttacgttcc cgctccgttt gtgttggatt
    33781 ctatcgttga agccgttgat tcaggcgtag gcttggtcgt tgtgattacc gaaggcgtac
    33841 ctactttgga catgctcaaa gccaaacgtt atttggaaac caacggcaac ggtacccgtt
    33901 tggtcggccc taactgcccg ggcgtgatta ctccgggcga gtgcaaaatc ggtattatgc
    33961 cgggccacat ccacactccg ggccgcatcg gtattatttc ccgttccggt acattgactt
    34021 acgaagccgt ggcacaaacc accaaactgg gcttgggtca atcaacctgt attggtatcg
    34081 gcggtgaccc gattcctggt atgaaccaaa tcgacgcact gaaacttttc caagaagacc
    34141 cagataccga cgccatcatc atgatcggtg aaatcggcgg tactgcggaa gaagaagcag
    34201 ccgaatacat ccaatccaac gtaaccaaac ctgttgtagg ttacattgct ggtgttaccg
    34261 ctcctaaagg caaacgtatg ggccacgccg gtgcgattat ttccggcggc aaaggtactg
    34321 ccgaggaaaa attcgccgct ttcgaaaaag ccggtatcgc ttacacccgc agccctgccg
    34381 agttgggcac gacgatgctg gaagtgttga aggcaaaagg tttggcataa tcaggtttga
    34441 caactgatta aacatcaaat gccgtctgaa accggaaatc gggtttcaga cggcattttg
    34501 tttgtcattt caaaaagagg cagcctcaac atacccacat tattttttgc cctttgaggc
    34561 agtcagaata tttatcggtc aaacaggagc aaacccgtaa gtcaggttgc acatattttt
    34621 ttgaagtcag ggaatgataa gtatcgcatt tgaaaagact gatattattt ttgcaggatt
    34681 ctgttttagc acgcgacatt ctttgtttga taggattaga atggatatag tccgcgtaac
    34741 actgcaaatc cgtttcatcg cgcacggtgt gttcgtaaaa acgccgttgc caaataccgc
    34801 gttcgttccg ccgctgtttg ccggcggata agtttttagt ataaggggaa tatgcggaga
    34861 atttggtttt aatcagccgc cggagtaggg aataatccgc atcgtcgggc ggcagcgtcc
    34921 aaatggcgtg gatatggttc ggcagcacgc atacggcgac ggtttcaaag ggatattgtt
    34981 tttgcacatc catataagcc gtacgcgaag ccgatatgtt cgacaagcag gcgcgatttc
    35041 gggtcggcga gtttgacggt gaaaaagaat gtgccgccgg caatgaagtt tctgcgataa
    35101 cgcgccatag gatttttgtt tgtggtttgg gtgttggaga aggaatggtt tgttgtttaa
    35161 ggttttaact ttgttggagc tgatgttttc agacgacatt ggaggccgtc tgaaagcgct
    35221 ggcttcaacg aagttaaaag ttggaacggc aaaccgttaa tcaaccaacc gcgtacgtac
    35281 cgcacacaca ctgcacgttg gctttgaagt ttcgtagact cacaggtagg tgtggcgcaa
    35341 ccacgcatac ggtgggttgg gtataaaggc tacaacttgc tatagcttgc ttgctattca
    35401 aaataagaat gcttaattgc tgttgctaat tgagcagcaa ggatgggagg aacggcatta
    35461 ccaacctgcg tgaattgccg cgcaaccgct cctgtaaaaa tataatcatc aggaaacgtt
    35521 tgtaatcgtg cactttcacg aacagataac gttcttggga tatatggatg taagtgagac
    35581 cttcctccac ctttagtgcc accagcaata atagttttag atggtttgct tggatctaac
    35641 ctatctacac gtcccttttt atcacgttgc ccataagcta attccatata gcgtaaaatc
    35701 gattcagccg aatgttctct tgtaatatgg ttatgttggt taataattgg aatttcaaat
    35761 gtattaccac atgccaatgg ggtattggtt ggttgtagcg gactaaattt tttatctcct
    35821 aaataaccaa ttataaatag cctaattctc tgttgaggta catgaaaata ttcagaatta
    35881 attatttgag gtttatagag tgagtagcct tgctgctgta aatcacaata tacactagct
    35941 aactgtttac cattatctat atccatcaat ccagagacat tttcaataag aaatacttta
    36001 ggacgaaaaa tcttaatcaa ttcaacgtaa ttaaataata gattaccatt ttgcttatgc
    36061 tcaaagccga cacgtttaaa atttttatca gtttttgaga atctctggtt tgaggctatg
    36121 gagaaaggct gacagggagg acctccaata aatacaccat caaaacttaa agacccaata
    36181 ttatttctga ttagttcaac catttcttct gtttcagaaa catcagcatt aaacacagac
    36241 caattagggc gattaagacg caatgtatca caaaataaag gtattttttc aatcagcata
    36301 gaatgttgaa agcctgctgc ttcaaaccct aagtctaatc caccggctcc tgaaaaaaaa
    36361 ctaattatcg gaatgtgatt ttcctttttt tgaaaatcac ttagaaccaa attctttctc
    36421 ttttcaaaag gctttttatc atgatgaata taaattaaat catcatagct catttgattt
    36481 tggcgtatta gttcattcaa tggaatacct tgtaataaac tttgttctaa taattttctt
    36541 tgtgaaataa ttctattatt ttcttggtca aataaagagg tttgcggtat taacatagat
    36601 actcctatct atttataaat tcttttaact ttacttttag cttcttcata tacatgatat
    36661 gcttcttcat aactaacaaa atcttttttc ttgtgcaaat taagccaaaa agtatagtac
    36721 ttgtgtgtgg cacgatgaca ggttggacag ataccaacca aatcttttaa tgaagtagtt
    36781 ttatcttcta cccttacagg agaagatagt ggtaatagat ggtgtacttc aatgatatgt
    36841 tcgctccatt catagctcct ttgcgtatct aatccacaca tgcgacatat ttgtgcattt
    36901 tgggtcttgc taaaatagat ttttcttaaa atactattac gttctgtaat taaatgggtg
    36961 actttaattt taccaccctc actaaacgcc tgatcatcaa tagataatga aacgatttca
    37021 ttcatcaata agaaattatt atttgtctta cccaatctca ttatctctaa actatctaat
    37081 ctttcttgtg gttgtgtatc tgcaataaat tggttttcta ataaatgtag ttcactaaaa
    37141 tctgtaatat ctaaaaacaa cttattttca taccaagaca aaaaagagaa ttgactaata
    37201 aactttacca actctctaat ttgacgagga tcatatttaa ttgcttcttt attcataatt
    37261 aactgtgaga atgaaaaaga agcaatacct gtaatactat tattactagc gattttgtaa
    37321 ataatttcat ccatgtccaa aaaattaact cctgtttcct tatattttga aattaaagct
    37381 ctaataattg ccaaaacagg gaaaacccga ttttcagatg gttgatatcc tgaaaaagct
    37441 ggtgatgggt aggaaaaatg tgttgcaaaa tgtagtaaat aatcatcgca actaatttca
    37501 tcattctgaa ctaaatcggc acataaatct gtagtacaga ttaacccatt atgctccgtt
    37561 gcgagtaaca aacatttaaa aacacgacca taattacgcc aaactggata ttgtgaactg
    37621 ttaggggcaa aaggtaagtt tgtctcttga gtcaggctta atcttacaat atcaatatct
    37681 tttgcacgtg gctttggaca ttgattaatt ccatataatc cttttgcaat ttttttaatt
    37741 tcatcaaatc taaaataatc taatctacct tgatcaaaac gccattcaat catttttaac
    37801 ctctatgaaa tatcattttt cagtaatctg aaaattatcc taaaaccacc ttcaaatact
    37861 tcccagtata actcccctta accttcgcca cttcctcagg gctaccttgg gcaataatcc
    37921 tccccccgct atcgccacct tccggtccta aatctacaat ccaatccgca gttttaatca
    37981 catccagatt atgctcaata atcacaatcg agttgccttt gcctttcaga cggcctatga
    38041 cttccagcag cagggcgatg tcggcgaagt gcaggccggt ggtgggttcg tcgaggatgt
    38101 agagcgttct gccggtgtcg cgtttggaga gttccaaggc gagtttgacg cgctgggctt
    38161 cgccgccgga gagggtggtg gcggactggc cgaggcggat gtagccgaga cctacgtcca
    38221 tcagggtttg cagtttgcgc gatacggtgg ggacggcgtc gaaaaattcg cgggcttctt
    38281 cgacggtcat gtcgaggact tggctgatgt ttttgccttt gtattggatt tcgagggttt
    38341 cgcggttgta gcgtttgccg tggcagactt cgcaggggac gtacacgtcg ggcaggaagt
    38401 gcatttcgac tttaatcacg ccgtcgcctt ggcaggcttc gcagcggccg cctttgacat
    38461 tgaaggagaa tctgccgacg ttgtagccgc gttcgcgcga gagggggacg ccggcgaaga
    38521 gttcgcggat gggggtgaac aggccggtgt aggtggcggg gttggagcgc ggggtgcggc
    38581 cgatggggga ttggtcgacg ttgatgactt tgtcgaggtg ttcgaggccg cggatgtcgt
    38641 cgtatggggc gggttcttct tgggcgcggt tgagttcgcg ggcggtgatt ttggcgaggg
    38701 tgtcgttaat cagggtggat ttgccgctgc cggatacgcc ggtgatgcag gtaatcaaac
    38761 cgagcggcag ttcgagggta acgtttttga ggttgttgcc gcgcgcgcct ttgaggacga
    38821 gcatccggtc gggattgacg ggtgtgcgtt cagacggcac ggcaatggat tttttgccgc
    38881 tgaggtattg tccggtaatg gagttttcgc atttggcgac gttttcgggt gtgtcggcaa
    38941 tcagtacgtt gccgccgtgt tcgccggcgc ccgggcccat atcgacgacg aaatcggctt
    39001 cgcggatggc atcttcgtcg tgttcgacca caatcacgct gttgcctaaa tcgcgcaggc
    39061 gtttgagggt ggcgagcagg cggtcgttgt cgcgctggtg caggccgatg gagggttcgt
    39121 ccaaaacgta cattacgccg gtcaggccgc tgccgatttg gctggcgagg cggatgcgct
    39181 gggcttcgcc gccggaaagg gtttcggcgg aacgggagag attcaggtaa tccagcccga
    39241 cattaatcaa aaagccgaga cgttcggtga tttctttgag gattttttcg gcgatttgtt
    39301 ttttgttgcc gtctaaatcc agtgtttcaa agaattggtg ggttttggtg agcggccagg
    39361 cggaaacttc gtgcaacggc tcaccgctga cgtaaacgta gcgggcttct ttgcgcaaac
    39421 gtgcgccgcc gcagctcggg caggcgcggt ggttttggta ttcgcgcagt ttttcgcgca
    39481 cggtttcgct gtcggtttcg cggtagcggc gttcgagatt ggggatgatg ccttcgaagg
    39541 cgtggctgcg gttgaaggtg gtgccgcgtt cggacaggta agtgaaatca atgacttcct
    39601 tgcccgagcc gtgcaacacg acttttttga tttttgcagg tagggtttcc caagcagcct
    39661 gcacgtcgaa accgtaatgc cgcgccagcg actgaatcat ttggaaatag aactggttgc
    39721 gcttgtccca gccgtcaatc gcgcccgaag ccagcgacaa ttcgggatgc gcgaccactt
    39781 tttcggagtc gaagaaattg gtgttgccca agccgtcgca agtcgggcag gagcccatcg
    39841 ggttgttgaa cgaaaagagg cgcggctcca attcgggcag gctgtacgaa catacggggc
    39901 aggcgaaacg cgcggaaaac caatgctctt cgccgctgtc catttccatc gccagcgcgc
    39961 gctcgttgcc gtggcgcagc gcggtttcaa aactttccgc cagccgctgc ttgatgttcg
    40021 ccttcacttt cacgcggtcg atgaccacgt cgatattgtg tttgatgttt ttttccagct
    40081 tcggcacttc gtcaagctga tagacctcgc cgtccacgcg cacacgggca aagccttgcg
    40141 cctgcaagtc agcaaagaag tcgacaaact cgcccttacg ctcacgaact gccggcgcaa
    40201 gaatcatcac gcgcgtgtct tccggcagtt ttaatacggc atcgaccatc tgagatacgg
    40261 tttggctgga tagcggcaga ttgtgttcgg ggcaatacgg cgtgccgacg cgtgcgtaca
    40321 aaaggcgcag gtaatcgtgg atttccgtaa ccgtgccgac ggtggaacgc ggattgtggc
    40381 tggtggattt ctgctcgatg gaaatcgcgg gcgacagacc ctcgatcaaa tcgacatcgg
    40441 gtttgtccat catctgcaaa aactgccgcg cataggcgga caggctctcg acataacgcc
    40501 gctgtccttc ggcatacagc gtgtcgaacg ccagcgatga tttgccgctg cctgacaatc
    40561 ctgttaccac caccagcttg tggcgcggaa tgtctaaatc gacgtttttc aaattatgcg
    40621 tgcgcgcgcc gcgtatgcgg atggtgtcgt tgtcgtgcga atgttgggga tgatggttgc
    40681 acataatgga tgccgcctga aaaataaagg aaaactggta ttgtagcact ttctcggatg
    40741 ccgtctgaag ccgcgttcag acggcatttg tcggcggagt acggcagatt ccgctataat
    40801 gtcggcaatt ttaacccgct tgaacaaaag gatgacaaat gaaccgtctt tacccccacc
    40861 cgattatcgc ccgtgagggc tggccgatta ttggcggcgg tttggctttg agcctgctgg
    40921 tgtcgatgtg ttgcggctgg tggtctttgc cgttttgggt gtttaccgta tttgccctgc
    40981 agtttttccg cgaccctgcg cgtgagattc cgcaaaatcc tgaagcagtg ttgagtccgg
    41041 ttgacggccg tatcgtggtg gtcgagcgcg cacgcgatcc gtatcgtgat gtcgatgctt
    41101 tgaaaatcag tatttttatg aacgtgttca acgtgcattc gcaaaaatcg cctgccgatt
    41161 gtacggtaac gaaagtggtc tataacaaag gcaaattcgt gaatgcggat ttggacaaag
    41221 ccagcacgga aaacgaacgt aatgcggtgt tggcgactac ggcttccggt cgtgaaatta
    41281 cttttgttca agtggccggt ttggtggcgc gccgtatttt gtgctacacc caagcaggtg
    41341 cgaaactgtc ccgcggcgaa cgttatggct ttatccgctt cggttcgcgc gtggatatgt
    41401 atctgcctgt cgatgcgcag gcgcaagtgg cgattggcga taaagtaact ggcgttagca
    41461 ctgtattggc gcgtttgccg ctgactgcgc cgcaaatcga atccgagcct gagtctgagc
    41521 ctgctttaca aactgctccg gttgaaacag cggcaaaccc atctgccgaa caacggcaaa
    41581 tcgaggcagc ggcggctaag attcaggcgg ctgtgcaaga tgtgttgaaa gattaatttt
    41641 gcgaactgaa atagaaaata tcagtaccat cattcacacg aatgaggaag tttggttttt
    41701 tgaatttttg ctaatgttca caccgttatg ttcacgaaaa tgggaatctg gaaatttaac
    41761 gttacggtga tttatcagaa atatagtgga t
//
LOCUS       DAXIHS010000004        40915 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXIHS010000004 DAXIHS010000000
VERSION     DAXIHS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40915
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.004"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(1..1527)
                     /locus_tag="ACFOF2_000167"
     CDS             complement(1..1527)
                     /locus_tag="ACFOF2_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC7106559.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(1639..3045)
                     /locus_tag="ACFOF2_000168"
     CDS             complement(1639..3045)
                     /locus_tag="ACFOF2_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC7106560.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFILGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            3329..4108
                     /locus_tag="ACFOF2_000169"
     CDS             3329..4108
                     /locus_tag="ACFOF2_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC7106561.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            4105..4806
                     /locus_tag="ACFOF2_000170"
     CDS             4105..4806
                     /locus_tag="ACFOF2_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC7106562.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            4803..6257
                     /locus_tag="ACFOF2_000171"
     CDS             4803..6257
                     /locus_tag="ACFOF2_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC7106563.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6440..6925)
                     /gene="purE"
                     /locus_tag="ACFOF2_000172"
     CDS             complement(6440..6925)
                     /gene="purE"
                     /locus_tag="ACFOF2_000172"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC7106564.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(6973..7689)
                     /locus_tag="ACFOF2_000173"
     CDS             complement(6973..7689)
                     /locus_tag="ACFOF2_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC7106565.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(7689..8357)
                     /locus_tag="ACFOF2_000174"
     CDS             complement(7689..8357)
                     /locus_tag="ACFOF2_000174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7106566.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8368..8553)
                     /locus_tag="ACFOF2_000175"
     CDS             complement(8368..8553)
                     /locus_tag="ACFOF2_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC7106567.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8696..10672
                     /gene="mutL"
                     /locus_tag="ACFOF2_000176"
     CDS             8696..10672
                     /gene="mutL"
                     /locus_tag="ACFOF2_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC7106568.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVVSTPMPSENDSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAINTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10767..12890
                     /gene="dnaX"
                     /locus_tag="ACFOF2_000177"
     CDS             10767..12890
                     /gene="dnaX"
                     /locus_tag="ACFOF2_000177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC7106569.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQASAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEA
                     FAHEAPAEPFYGYGFPDNDCPPEDGAEIPPPDWEHAAPADAEEENNADEGSNDENNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTQDWHDESGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    11956..11995
                     /gene="dnaX"
                     /locus_tag="ACFOF2_000177"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            12970..13305
                     /locus_tag="ACFOF2_000178"
     CDS             12970..13305
                     /locus_tag="ACFOF2_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC7106570.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(15590..16636)
                     /gene="recA"
                     /locus_tag="ACFOF2_000179"
     CDS             complement(15590..16636)
                     /gene="recA"
                     /locus_tag="ACFOF2_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC7106571.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            16890..17654
                     /gene="aroD"
                     /locus_tag="ACFOF2_000180"
     CDS             16890..17654
                     /gene="aroD"
                     /locus_tag="ACFOF2_000180"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC7106572.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            17674..19689
                     /gene="rep"
                     /locus_tag="ACFOF2_000181"
     CDS             17674..19689
                     /gene="rep"
                     /locus_tag="ACFOF2_000181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC7106573.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(19757..20815)
                     /gene="dinB"
                     /locus_tag="ACFOF2_000182"
     CDS             complement(19757..20815)
                     /gene="dinB"
                     /locus_tag="ACFOF2_000182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC7106574.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYDSEVAKEIRAAIFAETGLTASAGIASNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(20959..24048)
                     /locus_tag="ACFOF2_000183"
     CDS             complement(20959..24048)
                     /locus_tag="ACFOF2_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC7106575.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPG
                     AGGKQGSFQTVAKPIGKVVSRGAEAELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            24322..25101
                     /locus_tag="ACFOF2_000184"
     CDS             24322..25101
                     /locus_tag="ACFOF2_000184"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC7106576.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSIRFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYVMHRNRIPGQIMMENGF"
     gene            complement(25311..26696)
                     /locus_tag="ACFOF2_000185"
     CDS             complement(25311..26696)
                     /locus_tag="ACFOF2_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC7106577.1"
                     /translation="MIVTSRWPLLEKAFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGRLHAHK
                     VKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFS
                     NKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACDRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGKTVVLHGMGGALEMDDGLWYFDKDLPDVFKAKF
                     KTDRKNIKEIG"
     gene            26814..26890
                     /locus_tag="ACFOF2_000186"
     tRNA            26814..26890
                     /locus_tag="ACFOF2_000186"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26848..26850,aa:Arg,seq:acg)
     gene            26908..26982
                     /locus_tag="ACFOF2_000187"
     tRNA            26908..26982
                     /locus_tag="ACFOF2_000187"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26941..26943,aa:Glu,seq:ttc)
     gene            27542..28717
                     /locus_tag="ACFOF2_000188"
     CDS             27542..28717
                     /locus_tag="ACFOF2_000188"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC7106578.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(29059..29607)
                     /locus_tag="ACFOF2_000189"
     CDS             complement(29059..29607)
                     /locus_tag="ACFOF2_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC7106579.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(29610..31106)
                     /gene="ccoG"
                     /locus_tag="ACFOF2_000190"
     CDS             complement(29610..31106)
                     /gene="ccoG"
                     /locus_tag="ACFOF2_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC7106580.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRVSVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            31634..33613
                     /gene="tkt"
                     /locus_tag="ACFOF2_000191"
     CDS             31634..33613
                     /gene="tkt"
                     /locus_tag="ACFOF2_000191"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC7106581.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            33754..35142
                     /gene="fumC"
                     /locus_tag="ACFOF2_000192"
     CDS             33754..35142
                     /gene="fumC"
                     /locus_tag="ACFOF2_000192"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC7106582.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(35207..36130)
                     /locus_tag="ACFOF2_000193"
     CDS             complement(35207..36130)
                     /locus_tag="ACFOF2_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC7106583.1"
                     /translation="MENQRPHLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(36655..37179)
                     /locus_tag="ACFOF2_000194"
     CDS             complement(36655..37179)
                     /locus_tag="ACFOF2_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC7106584.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(37182..38564)
                     /locus_tag="ACFOF2_000195"
     CDS             complement(37182..38564)
                     /locus_tag="ACFOF2_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC7106585.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(38675..39298)
                     /locus_tag="ACFOF2_000196"
     CDS             complement(38675..39298)
                     /locus_tag="ACFOF2_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC7106586.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            39358..39597
                     /locus_tag="ACFOF2_000197"
     CDS             39358..39597
                     /locus_tag="ACFOF2_000197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106587.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAETFAKIVC"
     gene            complement(39985..40406)
                     /locus_tag="ACFOF2_000198"
                     /pseudo
     CDS             complement(39985..40406)
                     /locus_tag="ACFOF2_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40443..>40915)
                     /locus_tag="ACFOF2_000199"
     CDS             complement(40443..>40915)
                     /locus_tag="ACFOF2_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC7106588.1"
                     /translation="RNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRERYPFEIL
                     AWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEYTIRDEAD
                     FAWYFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSIGYD"
BASE COUNT        10160 a        11521 c        10336 g         8898 t
ORIGIN      
        1 ctataataaa ctttctatgg gcagcaggga taggattttt gcggcgatgc gtttccaaag
       61 tttggcttcg ggttcgttcg ggtaggtttt tcgggtggcg ggatcgtgcc attgcaggcg
      121 gttgtgcctg tcgagggtaa cgcggtaggc gtattcgggg gtggtatcgg caagggtgcg
      181 ctccatctgt tctgcgattt tggggctttc gataacaacg cccatttcgg tgttgagacg
      241 cgcggaacgg gggtcgaggt tgaacgaacc gatgaagatg cgtttgccgt ccacaatgaa
      301 ggttttggcg tgcaggctgg ttacggagct gccggtcagg cctttgtctt ttgtggcagg
      361 gacggcatgg ttgggttgca gctcgtagag tttgatgccg gctttgagca gcggttttcg
      421 gtatttgaca tagccggaat ggacggcggc aacgtcggtc gcctgtagcg agttggtcag
      481 gacagtaacg tctatgccgt cctgcaccag ttttgctagt gcgtctgtgc cggattttgt
      541 ggggacgaaa tagggtgaaa ccagatagac gcttttttcg ggctgtttga gcgcgtcttg
      601 cagccgcccg gcaatcggcg gtttgcggcg gtcgcggtcg agtccttttg cagggtcgtc
      661 gctgatgagg cgggtttgga cgctctgcca gtcgatgcgt cctgcctgta ttttttggta
      721 gaggggcgac tgttcgacgg tttcgcggta gcgcaggagc gcgtgtctgg acgtttcgtc
      781 gttgtatccg agtgcttgaa gacccttgcc gatgttgccg ctgcggatga tgcgcgtggc
      841 gttgtgggcg gaatggcttg cccagtagcg gtcgaagtcg tgcgatactt cgccgacgac
      901 gctgccggtg gcgaggatgt ccaggtcggc gaaaacggtg tcctcaccga ctttgaagta
      961 ttcgtcgccg atattgcgtc cgccgagtat ggtggcgcgg ttgtcggcgg taaaggattt
     1021 gttgtgcatg cggcggttga ggcgggggaa gtcggtcagg tagccgagtg cgcgccattt
     1081 tcgtaagacg aaggggttga acaggcgcac ttcgatattg ggatggctgt cgagggcgag
     1141 caggaggtcg tccaatccgc gcgtgttgtt gtcgtccaac agcaggcgta cgcgcacacc
     1201 gcgttctgcg gcaaggtaca cgaggttgaa cagcagtcgg ccggaaatgt cgttgcgcca
     1261 gatgtagtat tgcaaatcga ggctgtgttc ggcagattcg ataagggcgg cgcgggcggc
     1321 aaaggcttcg tgggggtcgt tcagcagata gatatcggat agcccgttgg tatgaggggt
     1381 gtgccggatt tgcaggatgt tgtccaggcg gacgggtttg gaagtattga aatgacggct
     1441 ttccgtccgt tcttccagtg ggggcaacca tgaagaacat gaacagagaa ggaggcataa
     1501 aagggaaatt aggctgcgtg ttttcatcag ggatatggtt tcagacggca ttgcctgtgt
     1561 tttggggttg gcgcgcatgg atgtgcggaa tcataatcca aacgttgaaa cgggtaaaag
     1621 ttttgcgtgc ggaccgcttt aggacagtgt gttccgtgtc aggtttgcgc cgtctgaaac
     1681 gtgcagacgc ttgaaaacca acgacgaggc aagggtgatg ccgccgatgc tgagcagggt
     1741 catacggaag gcggaatgca gacctgaaga agccggtatc agaaatgtcc agtttttaag
     1801 gattaatgcg ccgacaacaa tgcccatgct gatggcaagc tgttggttga ccgccatcag
     1861 gctgttgccg ctgccggtct gttgcgggcg caaatcggcg agggtcagtg tattcatggc
     1921 agaaaactgc agggagttgc acgcgccgat cgccagcgag aggaaaatcc aaatccacag
     1981 cgacgagttt ccgtcaggca gggcgagcag catgatgaag gcggcaagca gcttggtgtt
     2041 ccaaagcagt accgtgcggt agccgaaacg tttcatgagc ggtgcaatca gcggtttgac
     2101 cagcagcgaa gacagggcga cgggggcgac cagccaaccc gacaggcttg cgccgaagcc
     2161 gaaagcgact tgaaacatca ggggcatcag aaaaggaatc gagctgatgc cgagacggct
     2221 gaacagattg cccgccagtc ctagacggaa agtgcgtatc agaaacaggt cggcggaata
     2281 aatcggtttg gacgcggttt tcatatgtcg gaaataacgg cgtgcaaaca gcagtccgcc
     2341 gcacagcggc aacagtgcaa aatacggagg cagcgcgtgc gacaggcttt ctgccgaaag
     2401 taacaagagg cacgcggcgg cagaaaaaat cagatagcct ttgaagtcta aagagatatt
     2461 gctgccttta atatcgggca tgatgttgcg tcccaatatg aaacccagca gaccgatggg
     2521 caggttgagc aggaaaatcc agtgccacga agcgtattcg accaaataac cgcccgccaa
     2581 aggccctaaa accggcccga ttaatgcagg catgaccgca taattgatgg cattgagcag
     2641 cttggacttg tcgtacatac gcaagatggt cagacgcggt atcggaacca gcatcgaacc
     2701 gccgatgccc tgaacgacac gggaaagcgt caattcaaac agcgaacccg atgcggcgca
     2761 caatgccgat ccgagtataa aaacggcaat cgaaccgaaa aagacttttt tcgttccgaa
     2821 cctgtccgcc aaataaccgc tcaaaggaat cagcagggca accgtcagcg tataggcaac
     2881 gactgccagt tgcatatcca gaggcgattc gtccaggtcg gtggcaattt caggcagtgc
     2941 ggtatttaaa atggtcgcat ccaacatctg cataaaaatg gcaattgcca gcagaagcgg
     3001 cagccaaggg gatggtgcgc gggcggatag ggtgtttttt tccatagggc gattgtaccc
     3061 catccttgtg ccgttattgt tttcagatgc tgtctgaatg ccgtcagagt cggcgtcctg
     3121 aatgttcaca agcaaacgaa tcggcatttc attgtaatga taattattat cgaaaattat
     3181 cagagtaagg tacagtaagc gttatggggc agtttgtaag aaaaaccgga ttatttttta
     3241 aaattagact tgacccgcaa cagtcaatta cttaaagtaa acgcttacct ttctacagaa
     3301 aaaaacgggt ttcccgttat caaaaaacat gagcgcaacc attcccccaa aaatcatccg
     3361 atacgacagc aatccgaccg atgtctattt tttcggcact tgcgtccttg atctttttat
     3421 gcccgaagca ggcatggatg ccattaccct aatcgagcaa cagggcatac gcgtccattt
     3481 cccgatggcg caaagctgct gcggtcagcc cgcctattca tccggccatc cgaccgaagc
     3541 ctttgatgtc gccagagcgc aactcgacct tttccccgag gagtggccga ttgtcgtacc
     3601 gtccggttcg tgcggcggta tgatgaagca ccattggccg acgctgttta aaggcagcga
     3661 gtacgaggaa agggctgtgg attgcgccgg ccgcatcatc gagtttaccc atttcctgct
     3721 tgccatcggt ttcaaacccg aagacaaggg cgaaccgctc aaagtcgccg ttcacacttc
     3781 ctgcgccgcc cgccgcgaaa tgaatgtcca tctctcgggc tggcaactga ttgacggtat
     3841 ggaaaacgtc gaacgcatcg tccacgacca cgaaagcgaa tgttgcggct tcggcggcac
     3901 attctccgtc aaacaagccg atatttccgg cgcgatggta accgacaaag tcgccgcgct
     3961 gaaagaaacc ggcgcaaccg aaatcatcag cgcggactgc ggctgtatga tgaacatcgg
     4021 cggcaaaatt gctaaggacg aaccgaatat gccgcgtccg aaacatatcg catccttctt
     4081 gttggaacgt accggaggca aagcatgagc gcgcgcgaaa atattttggc aaaactgaaa
     4141 aaagccgacg cattgccgat ggaagaacct gcggtttttg attattaccg tgaaatgggt
     4201 gtttcttggg gcagcgaagc cgagcgtctg aaacattggg ctgccgcaat gcgcgcggtc
     4261 aaaaccgaaa tttattgggt gacgaaaagc aattggatgc aggttttccg cgaagcggca
     4321 gaaggcaagg gtttgaaaaa catcctgctg cccttggcga ccgaacacgg acaaattgcc
     4381 cgtgccgcat tggcggacag caatatcgaa ccgattgcct tcgagcgcga aatcgatact
     4441 tggaaaaccg agtttttcac gaacatcgat gcgggcttca gcggcgcgca atgcggcatc
     4501 gcccgcaccg gcacgctgat gctgttttcc agccccgaag agccgcgtac tttgagtctc
     4561 gttccgcccg tgcatttctg cctgttcgat acgtccaaga tgtacaacga gtttcataat
     4621 gccgtcgaag gcgaaaaact ggtggaaaac ggtatgccga ccaatgtatt cctgatttcc
     4681 ggtccgtcca aaaccgcaga catccaactg acgcttgctt acggcgcgca cggtccgcgt
     4741 gatttggtta tcctcgccat cctgcccgac cacatttccc ctaccgattt ggaggaaaac
     4801 gcatgactac gcaaaccatc aaatttcaca tgaagccgga aactttcaag caaaacgccg
     4861 caatttccct tcaagacaag cctttgcgta aaagcctgcg taccgcgatg gatatgctga
     4921 tgaccaaacg caaagctgtt ttgaccgacg aagaagagct gcaaagcttg cgcgatttgt
     4981 gcgaacacgt ccgtcagcgc tcattgtcta aattgcctgc cctgctggag cagctggaag
     5041 aaaacctgac caagttgggc gtaaaagtac actgggcgga aacaccggcc gaagcctgcg
     5101 aaatcatcca cgacatcatc acagccaaaa acggcaagct gatggtcaaa ggcaaatcga
     5161 tggtcagcga ggaaatcgag ctgaaccatt atcttgaagc aaaaggcatt aaagcggtgg
     5221 aaagcgactt gggcgagttc atcgtccaaa tggcaggtga aaaaccgacc catatcgtga
     5281 tgcctgctat ccacaaaacc aaagaacagg ttagcgaact gttccaccaa aacctcggta
     5341 cgccgctgac agacgatgta gaccaactga ccggcttcgc ccgtaaagca ctgcgcgata
     5401 tttacagcac tgccgatgtc ggtttgagtg gcgtaaactt tgccgttgct gaaacaggta
     5461 cgctgtgtct ggtggaaaac gagggcaacg gtcgcttgag taccaccgta ccgcccgtgc
     5521 atatcgctat taccggcatt gaaaaagtgg tggcgaaatt gtccgacatc ccacccttgt
     5581 acagtctgct gccgcgctcc gccatcggtc aaaacatcac aacctatttc aatatgatta
     5641 ccggcccgcg ccgcagtgaa gaattagacg gtccgcaaga aatgcacttg gttctgctcg
     5701 acaacggccg cagccaagct tacgcagaag atcaaatgcg ccgcaccttg caatgtatcc
     5761 gttgcggtgc gtgtatgaac cactgtccgg tttatacccg tatcggtggt gcggcttacg
     5821 gtacgactta tcccggcccg attggcgaga ttatctctcc gcatttgttg ggcttggatg
     5881 cgacacgcga tctgccgact gcgtgtacta tgtgtggcgc gtgcgttgaa gtttgtccgg
     5941 tacgcattcc gattaccgaa caaatgcaac gtctgcgtgt tgaagcgcaa cgttcgccga
     6001 ccgaaaccgt gccgcacccc atccgagggc aaggcgcatc gcataccttc ggcgaacaaa
     6061 tggcgtggcg cacattcaac ggtattttca gcggcagcaa aacctaccgc gccttcggtt
     6121 gggcagccac caagttccgc aacctgaccc cacgcaaaca gttgggttgg acgcaaaacc
     6181 gcgtgccgat gaaaccggcg aagaaaacct tgcacgaact aatggcagaa aaaatgcgcc
     6241 aaaaagaaca ggcataaaaa gttgttcgca aaaatgccgt ctgaaacccg aaacagggct
     6301 tcagacggca tttgtatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     6361 agtacaaata gtacggcaag gcgaggtaac gccgtactgg tttaaattta atccactata
     6421 tattcgcaga cggtgggttt taaatttgtt ccaattccat attcaaaaca gcctgttcct
     6481 gtttggctcg gaagtctgcc agtttttgcg ccagttcggg ggtttcgttg gcgagcatgg
     6541 aaacggcgaa caatgcggca tttgccgcgc ctgcctcgcc gatggcgaat gtggcgacgg
     6601 gtacgccttt gggcatttgt acaatcgata aaagcgaatc ttcgccgcgc aggtatttgc
     6661 tggggacggg tacgcccaaa acggggacgg tggtcttggc ggcaaccata ccgggtaaat
     6721 gcgccgcgcc gcccgcaccc gcgatgatgg ctttgatgcc gcgcgcccgt gcggtttcgg
     6781 cgtattggaa catcaaatcc ggggtgcggt gtgcggaaac aacgcgcgcc tcatattcta
     6841 cgccgaactc ttcaagaaac tgcgctgcct gccgcataac gggccaatcg ctgttgctgc
     6901 ccatgatgat gccgatttgt atcataaatc ctccttggtg cggatggggt aaaaagcgga
     6961 aaaatggaaa aactatcgtt tgcgcacggc tgcggcggcg cgttttgccg ccgggctgct
     7021 cgggtaagcc tgtatcaggc tgcgccaagt cgcccttgca atgtctttct gctgcaacct
     7081 gtattggcat tcgccgattt tgaacatggc ttcgggcgcg gttgggctgt ctttgaaacg
     7141 gttggcgtaa cgccctccga tttcgatgac ggattcgcag ttgcccatac gcgccctgct
     7201 ttgcagcaac agatacatac tgcgttgcgc gatgctgccg ccgtcgcctc cgtccgcgcc
     7261 tttcaacagg gaggcagcgg cagaaaacct gccgctttta tagtgtttga gtgcctgatt
     7321 gtagaggttt tgtgcggttt cgacggtatg tgcggatgcg ctgccgcctt cggtattgag
     7381 gtaatgctct ttcaacttgc ggtcgtcgag tttttggacg tatgccctgc tggaagggtg
     7441 tgttttcgca tgctccagtg ctttgacttt gccgtttaag gtttccactt cgttcgacag
     7501 gcggacgagt gtgccttcca gataatccaa acggtcttgc agagtgggaa cgggataggg
     7561 aatagcgtcc gaactacctg cctctgcctg cggttcgggc cgcatatcct gaatattgcg
     7621 ggaaacaggg gaagaacagg cggcagatac ggacagccaa atgataaaaa gcggtaattt
     7681 ggtcttcatt attttttcag aagcagggtc aagccgtcgc cgacgggcag ggtgatgggg
     7741 acaatccgcg tatcgttcgg caggttttga ttgaaatctt tgaggatgcc gacgctgggc
     7801 ggcgcatcgg aagccgcttc gcgcatcacc cttccgttca gcaaaatatt gtcgatggcg
     7861 atgatgccgc cttgacggac aagcttgagg caacgctcga aatatcgcgg cgtgggcggt
     7921 ttgtctgcgt ctatcagtgc caaatcgtag cttccggctt caccctgtgc aatcaaatca
     7981 tccaatgtca gcaatgcggg ttgcaggtgc aggctgattt tatgtgccac acctgcctcg
     8041 ttccaaacct gacgcgccgt atcggtaaag gttacattga tgtcgcaggc ggtaatccgc
     8101 ccgtgttctg gcaacgccaa tgcaagcgcg gtgctgctgt atccggtaaa cacgccgact
     8161 tccagatatt tttccgcgcg aatcagcttt gccagccaaa ccaaaaccgc cgcctgttcg
     8221 cgcgcaatcg ccattttgcc catacggtga tgctcggtct tctcacgcag ccgcgtcaaa
     8281 acgggatgtt cgggttcgcc gatgctgttc agatagtttt gcaggtccgg tgcgacattg
     8341 gacagatggg tcgtcatttc ggcgggttca gtcttggtaa taggtataag gttttttcgc
     8401 tacttttgcc gcctcgaagt tttcctgttc ttcgggattg agttcgacat cccacaaaag
     8461 cccccggttt tccaaacgct gctgttccaa ctcaggtttt tcttcaatca ggcggttgag
     8521 gaattgtgtg gcttcggatt ggtagtgata catctttgcg ctccaatttt acgggatacg
     8581 gcgtgattat actggtattt tccaaacggg ataaacggct tttatcaaga atacaggcag
     8641 aaaagtaagg ggttttatta tagaataaag cgtttttgca acggaagcct accttatgcc
     8701 acgcattgcc gccctgcccg accatcttgt caaccaaatc gccgccggcg aagtggtcga
     8761 acgccctgcc aacgccttga aagaaatcgt tgaaaacagt atcgatgcag gcgcaacggc
     8821 gattgatgtc gagctggcgg gcggcggcat ccgcctgatt cgcgtcagcg acaacggcag
     8881 cggtattcat ccggacgaca tcgagcttgc gctccaccga cacgctacca gcaaaatcaa
     8941 aaccttaaac gatttagaac acgttgccag catgggcttt cgcggcgaag gcttggcaag
     9001 catcgcctcc gtcagccgcc tgaccctgac cagccgtcaa gaagacagtt cgcacgcaac
     9061 ccaagtcaaa gccgaagacg gcaaactcag cagccccacc gccgccgccc accccgtcgg
     9121 caccaccatc gaagccgccg aactcttctt caacaccccc gcgcggcgca agttcctcaa
     9181 gtctgaagcc accgaatacg cccactgcgc cactatgctc gaacgcctcg cgctggcgca
     9241 tccgcacatt gccttctcgc tcaaacgcga cggcaaacaa gtgttcaaac tccctgaaca
     9301 aagcctgcac gaacggattg ccgccattgt cggcgatgat tttcagacgg catctttgga
     9361 aatcgacagc ggcagcggcg cattgcggct ctatggcgcg attgccaagc cgactttcgc
     9421 caaaggcaaa accgacaaac aatactgctt cgtcaaccat cgttttgtcc gcgacaaagt
     9481 gatgctccac gccgtcaagc aggcataccg cgacgttttg cacaacgccc tcacccccgc
     9541 tttcgtcctt ttcctcgacc tgccgcccga agccgtggat gtcaacgtcc acccgaccaa
     9601 aaccgaaatc cgcttccgcg acagccggca ggtgcaccaa ctcgtgttcc acacgctcaa
     9661 taaagccctt gccgacacac gcgccgactt gaccgaaagc gtcagcaacg caggcgaagt
     9721 gttgcatgac attaccggcg ttgtctccac cccaatgccg tctgaaaacg acagcgaaaa
     9781 tctgtttgat cgcgcatcca actacccgac aggcaacaaa tcagatacac acaatgcctt
     9841 tggttcatca ggcaaaaccg cgcccatgcc ctatcagtcc gcatatgcgc cgcaacaacg
     9901 cagcctgtcc ctgcgcgaaa gccgcgcggc aataaacacc tatgccgaac tctacaaaaa
     9961 aaccgacgac atcgaccttg agttaagccg attcgagcag gcacgtttcg gcaatatgcc
    10021 gtctgaaacg cctgctcccc aaacagatac gccgctttca gacggcatcc cgtcccaatc
    10081 cgaactgccg ccgctcggtt ttgccattgc ccaattactt ggcatctaca ttcttgccca
    10141 agccgaagac agcctgttgc tcatcgatat gcacgctgcc gccgaacgcg tcaactacga
    10201 gaaaatgaaa cgccaacggc aggaaaacgg caggctgcaa agccaacgcc tgcttattcc
    10261 cgtaaccttt gccgcgtccc acgaagaatg cgccgccctt gccgattatg ccgaaacgct
    10321 ggcaggcttc gggctggaat tatccgatat gggcggcaac accctcgccg tccgcgccgc
    10381 acccgcaatg ttgggcaaat ccgatgtcgt ctccctcgcc cgcgacgtat tgggcgaact
    10441 cgcccaagtc ggcagcagcc aaaccatcga agaacacgaa aaccgcatcc tcgccactat
    10501 gtcctgccac ggctcgatcc gcgccggccg ccggctcacc ctgcccgaaa tgaacgccct
    10561 tctgcgcgat atggaaaata cgccgcgcag caaccagtgc aaccacggca ggccgacttg
    10621 ggtcaaactg actttgaaag aattggacgc actgttcttg cgcggacagt aagccgaaag
    10681 tgctagaata cgccgcccga gaccgccgtt cagacggcat tccgacgcac cgacagaaac
    10741 atcacgaccg aaaccaagag aaaaacatgg cctatcaagt tctcgcccga aaatggcggc
    10801 ccaaaacctt ttccgaccta gtcggtcagg aacacgtcgt caaagccctg caaaatgcct
    10861 tggacgaagg caggctgcac cacgcctacc tgctgaccgg cacgcgcggc gtaggcaaaa
    10921 ccaccatcgc ccgcatcctt gccaaaagcc tcaactgcga aaacgcgcaa cacggcgaac
    10981 cttgcggcgt gtgccaaagc tgtacgcaga tcgatgccgg acgctatgtc gacctgctgg
    11041 aaatcgacgc cgcctccaac acaggcatcg acaacatccg cgaagtattg gaaaacgccc
    11101 aatacgcgcc gaccgccggc aaatacaaag tctatatcat cgacgaagtg catatgcttt
    11161 ccaaaagcgc gttcaacgct atgctcaaaa cgctggaaga gccgcccgaa cacgtcaaat
    11221 tcatccttgc cacgaccgat ccgcacaaag tccccgttac cgtattaagc cgctgcctgc
    11281 aattcgtctt acgcaatatg accgcgcagc aggttgccga ccaccttgcc cacgtcctcg
    11341 acagcgaaaa aatcgcctac gccccccccg ccctgcaact tttgggacgc gccgccgccg
    11401 gatcgatgcg cgatgccttg agcctgctcg accaagccat cgccctgggt tcgggcaaag
    11461 ttgccgaaca agatgtccgc caaatgattg gtgcggttga caaacaatac ctttacgaac
    11521 tgctgacagg catcatcaac caagacggcg cagccctgac cgccaaagcg cgggaaatgg
    11581 cggcgtgtgc cgtcggcttt gacaacgcct tgggcgaact cgccatactg ctgcaacacc
    11641 tcgccctgat acaggcagtg ccgaatgcct tggcgcacga cgaccccgat tccgatattt
    11701 tgcaccgcct cgcccaaacc ataggtggcg aacaaatcca gctttactac caaatcgccg
    11761 tccacggcaa acgcgacttg ggcctcgccc ccgacgaata cgccggcttt atgatgaccc
    11821 tgctgcgtat gctggcgttt gcgcccttgg cggcagcttc gtgtgatgca aacgccgtga
    11881 ttgaaaatac cgaacttcaa tccccatcgg cacaaaccgc cgaaaaggaa accgccgcaa
    11941 aaaagcccca accgcgccct gaagcggaaa ccgcccaaac acccgttcag acggcatccg
    12001 cagcggcaat gccgtctgaa agcaaaactg ccgaacccgt taccaatcaa gaaaacaacg
    12061 atgttccgcc ttgggaagac gcgccggacg aagcccaaac cgcagccggc acgaccgcgc
    12121 aagcatcggc aaaaagcatt cagacggcat ccgaagccga aacatcgcct gacaatcaag
    12181 tttccaagaa caaggcagcc gacaacgaaa ccgagacttc cttgtccgaa gtgccgtctg
    12241 aaaaccccat tcaggcaaca ccgaatgatg aagccgttga aacggaagca tttgcgcacg
    12301 aagcccctgc agaacctttc tacggttacg gctttccgga taatgactgc cccccggaag
    12361 acggcgcaga aatcccgccg ccggattggg aacacgccgc ccctgccgat gcggaagaag
    12421 aaaacaacgc cgacgaaggc agcaacgacg aaaacaacac gccgtccgcc ccgccgcccg
    12481 agttttccac cgaaaactgg gcagccatcg tccggcattt cgcccgcaaa ctcggcgcgg
    12541 cgcaaatgcc ggcgcaacac tccgcgtgga cggaatacca tcccgacacc ggcctgatgg
    12601 ttttggcaat gaccgccgaa gcacgcgcca ccgccgacaa aaaacgcctc gacaaaatcc
    12661 gcgacaccct tgcccaagcc tacgggctgc aactcaccct gcaaacccaa gactggcacg
    12721 acgaatccgg cagagaaacc cccgcaatgc aggacaagcg cgtccaagcc gaagacaggc
    12781 aaaaagcaca agcattgctc gaagccgacc ccgccgcaca aaaaatcctc caagcattcg
    12841 gcgcgcaatg gcagcccgaa tcactggaat tggcggcaaa ccggccataa acagatataa
    12901 tgccgcccga acccttcgga cggcattgcc gtttccctta ttcaatcaaa acagacagga
    12961 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
    13021 caggaaaata tgaaaaaagc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
    13081 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
    13141 agccccgatt tgattcaaga agccgccgac gacaaagaaa tgctcgaaga cctcatcctc
    13201 gccgccctca aatccgcccg tgacaaagcc gaagaaaccg caaacaaaac aatgggcgca
    13261 ttcacgcaag gtctaccccc cggagtgggc gacttcttcc gctgataccc gaccgtcatt
    13321 cccacgcagg cgggaatcta gaacgtagaa tctaagaaac cgttttactc gataaatttc
    13381 cgtgccgacg ggtctagatt cccgccttcg cgggaatgac ggcatcagtt tgcaggattc
    13441 ggcgtgaacg gtaaaaacag tgagaatgat aagaacgcaa aaacggcaag aatagcggga
    13501 atcggcaggc tgaagcccac cctaccatta tttacacatc cgtaccgctt aaatgccgtc
    13561 tgaaatttcg tcattcccat gaaaacggga atccagcccc gtgggagcag aaacttacac
    13621 cccgtcattc ccgcgaacgc gggaatccag taaccgaaaa accacaggaa tctatcggaa
    13681 aaaacagaaa cccccgaccg tcattcccgc gaacgcggga atccagtaac cgaaaaacca
    13741 cgggaatcta tcggaaaaaa cagaaccccc cgccgccgtc attcccgcga acgcgggaat
    13801 ccagtaaccg aaaaaccacg ggaatctatc ggaaaaaaca gaaccccccg ccgccgtcat
    13861 tcccgcgaac gcgggaatct agaacgtaga atctgagaaa ccgttttact cgataaattt
    13921 ccgtgccgac aggtctagat tcccgccttc gcgggaatga cggcatcaat ttgcaggatt
    13981 cggcgtgaac ggtaaaaaca gtgagaatga taagaacgca aaaacggcaa gaatagcggg
    14041 aatcggcagg ctgaagccca ccctaccatt atttacacat ccgtaccgct taaatgccgt
    14101 ctgaaacttc gtcattcccg tgaaaacggg aatccagcct cgtgggagca gaaacttaca
    14161 ccccgtcatt cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga atctatcgga
    14221 aaaacagaaa ccctcgaccg tcattcccgc gaacgcggga atccagtaac cgaaaaacca
    14281 cgggaatcta tcggaaaaaa cagaaacccc cgaccgtcat tcccgcgaac gcgggaatct
    14341 agaacataga atctgagaaa ccgttttact cgataaattt ccgtgccgag gggtctggat
    14401 tcccgccttc gcgggaatga cggcatcagt ttgcaggatt cggcggaaac ggtaaaaacg
    14461 gcagaatcga tgggaatcgg caggctgaag cccaccctac cattatttac acatccgtac
    14521 cgcttaaatg ccgtctgaaa cttcgtcatt cccatgaaaa cgggaatcca gccccgtggg
    14581 agcagaaact tacaccccgt cattcccgcg aacgcgggaa tccagtaacc gaaaaaccac
    14641 aggaatctat cggaaaaaac agaaaccccc gaccgtcatt cccgcgaacg cgggaatcca
    14701 gtaaccgaaa aaccacggga atctatcgga aaaaacagaa acccccgacc gtcattcccg
    14761 cgaacgcggg aatctagaac gtagaatctg agaaaccgtt ttactcgata aatttccgtg
    14821 ccgaggggtc tggattcccg ccttcgcggg aatgacggca tcagtttgca ggattcggcg
    14881 tgaacggtaa aaacggcaga atcgatggga tgcggcaggc tgaagcccac caaaacacaa
    14941 aaattccgat gccgtctgaa atttcgtcat tcccgtgaaa acgggaatcc agccccgtgg
    15001 gagcagaaac ttacaccccg tcattcccgc aaaagcggga atccagtaac cgaaaaacca
    15061 caggaatcta tcggaaaaaa acagaacccc ccgccgccgt cattcccgcg aacgcgggaa
    15121 tctagaacgt agaatctgag aaaccgtttt actcgataaa tttccatgcc gaggggtctg
    15181 gattcccgcg ttcgcgggaa tgacggcata ttttttgcat ttgatataaa gggtcgtttg
    15241 aattttgttc agcaagtgca aagtgttgca cataaaaggg cgcaggatag aggcaaagcg
    15301 ggcgtaggtc gggctgtagc aactgtattt ttcaccccgt cgggcaaaaa tatagtggat
    15361 taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggta
    15421 ctgaagcacc aagtgaatcg gttccgtact atctgtactg tctgcggctt cgtcgccttg
    15481 tcctgatttt tgttaatcca ctataccaaa actcaaatca agccgttcgg aggcggctca
    15541 aaaaaacggt acttcgcagc agaagtaccg tttatcggga tttcaggttt tattcttcgg
    15601 ggcgttcgcc gtcggtttcg tcctgcgtcc cttcggtgat gtgcatttct acgccgttga
    15661 gggcgcggat ttttgcgtcg atttcattgg cgacttcggg attttccttc agccagacgc
    15721 ggacgttgtc tttgccctga ccgattttcg cgccgttgta gctgtaccac gcgccggatt
    15781 tgttgatgat gtcgtttttc acgccgatgt cgatcaattc gccttcccaa ctgatgcctt
    15841 ctccgtagag gatgtcaaac tctgcctgac ggaacggggg ggcgactttg tttttgatga
    15901 ctttgacgcg ggtttcgttg cccaatactt cttcgccttt tttgatggat ccggtgcggc
    15961 ggatgtcgag gcggacggaa gaatagaatt tcagcgcgtt gccgccggtg gtggtctcgg
    16021 ggctgccgaa cattacgccg atcttcatcc ggatttggtt gatgaacaca accagcgtgt
    16081 tggttttttt gatgtgtccg gtcagtttgc gcaaagcctg actcatcagg cgcgcctgca
    16141 gtccgacatg gctgtccccc atatcgcctt cgatttcggc tttggggacg agtgcggcta
    16201 cggaatcgac gactaccata tctatgccgc ccgaacggac gagtgtgtcg cagatttcca
    16261 aagcctgttc gccggtatcg ggctgggaca ggtaaagctc ttcgactttt acgccgagtt
    16321 tgcgggcgta aacgggatca aaggcgtgtt cggcatcgac aaaggcgcac acgccgccgt
    16381 ttttctggca ttgggcgacg gcttcgaggc agagggtggt tttgccggag gattcggggc
    16441 cgaagatttc gacgatgcgc ccgcgcggca gaccgccgac tccgagggcg aggtctaatc
    16501 cgagcgatcc ggtggaaatg acttcgaggt tttcttcctg ctggctgccg tccattttca
    16561 tgatggcgcc tttgccgaaa cttttttcga tttgcgccag tgcggcggca agggctttgc
    16621 ttttgtcgtc tgacattggg gttactccgg aacaaatgcg gtatgtagga tgcggcgcaa
    16681 cacgggctgc ggcgcgggat gtgtatcgtt ttcccgatgt gcgggctatc ggtaatgctg
    16741 cttcacgagg ttgccattat cgcatatttc cttgcttgcc gatatgcggc aagacgcggc
    16801 ggcttgtgcc ggaatggaat ctggatgccg tctaaaaggc ggccggcttt gttataatgg
    16861 cggctgtttt ttctgtgtgt gcctgtttta tgtgttcctg ccttgttgtc aaaaataccg
    16921 ttatcggaag cggacgcacc aaaatcgccg tgccgcttgt cgcccgcgat gccgccgaac
    16981 tttccgccgt acttgaccaa atcaaaaacc tgcccttcga catcgtcgag ttccgcgccg
    17041 attttttgga atgcgcgggc agcatcggcg aagtattgcg ccacacgcag accgtccgcg
    17101 acgcgctgcc cgacaagccg ctgctgttta cgttcagacg gcatggcgaa ggcggcgcgt
    17161 tcccgtgttc ggacgattat tatttcaaac tgctcgacgc gctgatcgaa agccgcctgc
    17221 ccgacatcat cgacatcgag ctgtttgccg gcgaaaccgc cgtccggcgc gccgtggcgg
    17281 atgctcaaaa aaacggcatc gccgccctgc tctgcaatca tgagtttcac cgcacgccgc
    17341 cgcaagaaga aatcgtatgc cgtctgaaac agatggagga ctgcggcgcg gacatctgca
    17401 aaattgcggt gatgccgcaa agcgcggaag atgtgctgac tttgctttcc gccacgctcg
    17461 aagcgaaacg gcttgccgcc aaaccggttg ttacgatgtc gatggggcag acgggtgcgg
    17521 tcagccgcct tgccggacag gtgttcggct caagcatcac gttcggttcg ggaacgcaaa
    17581 actccgcgcc ggggcaaatc ggcgtatccg ccctccgtgc ggcactcgac tgtctcgaaa
    17641 gcggcgcaga ctgatttcag acggcatcaa aacatgatga aactcaatcc ccaacagctc
    17701 gaagccgtcc gttacctcgg cggtcccctg ctcgtcctcg ccggcgctgg cagcggcaaa
    17761 accggcgtga ttacgcaaaa aatcaagtat ttgattgtca atgtcggcta tctgccgcat
    17821 accgttgccg cgattacctt taccaataaa gccgccgcgg aaatgcagga gcgcgtcgcc
    17881 aaaatgctgc ccaagtcgca aacgcgcggg ctgacgattt gcacgttcca ttctttgggc
    17941 atgaagattt tgcgcgaaga ggcgaaccat atcggttaca aaaaaaactt ctccattctc
    18001 gattctaccg acagcgcgaa aatcatcggc gaactcttag gcggtacggg caaagaagcc
    18061 gtattcaagg cgcagcacca gatttccttg tggaaaaacg atttaaaaac gcctgaagat
    18121 gtcgttcaga cggcatcgaa catttgggaa caacaaaccg cacgcgtgta tgcgagctat
    18181 caggaaacct tacaaagcta tcaggcagtg gactttgacg acttaatccg cctgcctgcc
    18241 gtgctgttgc agcaaaacag cgaagtgcgc cacaaatggc agcggcggct gcgttatctg
    18301 ctggttgacg aatgccaaga tacgaatacc tgccaattta cgttgatgaa acttctgacc
    18361 ggcgcggaag gtatgtttac cgccgtcggc gacgacgacc agtccatcta cgcatggcgc
    18421 ggtgcgaaca tggaaaacct gcgtaaaatg caggaaaact atccgcagat gaaggtcatc
    18481 aaactggagc aaaactaccg ctccaccgcg cggattctca aaatcgccaa caaagtcatc
    18541 gaaaacaacc ccaagctgtt taccaaaaaa ctttggtcgc aattgggcga aggcgagccg
    18601 gtcaaagtcg ttgcctgcca aaacgagcaa cacgaagccg actgggtcgt cagccaaatc
    18661 gtcaaacaaa aactcatcgg cggcgacaaa acccaatatg ccgatttcgc cgtgttgtac
    18721 cggggaaagc atcaggcgag gattttcgag gaagcattgc gcggcgcgcg catcccctac
    18781 cgtctctccg gcggacaaag ctttttcgac aaagccgaaa tcaaagacgt gttgtcttat
    18841 gtgcggctgc ttgccaaccc caacgacgat cccgcctttc tgcgtgccgt taccacgccc
    18901 aaacgcggca tcggcgatgt aacgctgggc aagctcaaca cttacgcgca cgaacacgaa
    18961 tgcagcctgt atgaagccgc gcaaaacgaa gaagccctcg ccacgctgaa caataccaac
    19021 cgccaacacc tgcaagcctt tatggatatg ttcggcagtt accgtgccaa agccgaaacc
    19081 ggcgaagcgg gcgagttcat caacagcctg ctcgaagaaa tcgactatga aaaccacctg
    19141 cttgccagtg aagaaggcaa agctggcgaa atcaaatggc gcaacgtcgg cgatttggta
    19201 tcatggtttg cgcgaaaagg cggggaagac ggcaaaaaca tcatcgaact cgcccaaacc
    19261 gtcgccttga tgacgctttt ggaaggaaaa gacgaagaag aaaccgatgc cgtctcgcta
    19321 tccacgctac acgccgccaa aggtttggag tatccgtatg ttttccttgt cggttgcgaa
    19381 gaaggcgttt tgccgcacaa cgacagcatc gaagaaggca acgtcgaaga agaacgccgc
    19441 ctgatgtacg tcggcatcac ccgcgccaaa cgccaactca cactgaccca ctgcgtcaaa
    19501 cgcaaaaaac aaggcacatg gcagttcccc gaacccagcc gattcataga cgaaatgccg
    19561 caggaagatt tgaaaatcct ggggcgcaaa ggcggcgaac cgattgtcag caaggaagaa
    19621 ggcaggcgca accttgccga tataatcgga aggctcgaca acctaaaaaa aagcggcgcg
    19681 gcggattaaa ccggagccgc aatgccgtct gaaggcttca gacggcatat tttttggacg
    19741 gcgcgcgtaa agcggtttac gcccacaaat cctgctgctg gtttttcggc acaagatgcc
    19801 ccacgccgat accgataagg cggaacgcgt cttccgtctg cggcgggacg cgcgccatca
    19861 acatttgcgc cgcctgaagc agggaggcgc agtcgggcaa tacggaggaa taagtcagcg
    19921 tgcgcgtgat gatgcggaaa tcgtaggtct tcagcttgag cgttacactt tgggcttcga
    19981 cgtttttgcg cgtgatttgc cgccacaagt cttcggcaag atggggcaag tgtccggcag
    20041 cctgctcgag cggcaggtct tcgggcaggg tgatttctgt ggagatttgg aggcgttcgc
    20101 gttcggcttt gacggggcgt tcgtccgtac cgcgcgccaa atcatagagg cggtagccgt
    20161 agcgtccgaa atggtttaag agctcgccgc gctcgaaacg gcgcaaatca cccgccgtct
    20221 gcatacccag cgactgcatt tttttcagcg ttaccttgcc cacgccgggg attttgccca
    20281 aaggcagggt ttccaaaaat gccatgactt tgtgcggcgg caacacaaac tgcccgttcg
    20341 gcttgcgcca gtccgacgcg attttcgcca gaaatttgtt cgacgcgatg cccgcggatg
    20401 cggtcaaacc cgtttccgca aaaatggcgg cacggatttc tttggcaact tcactgtcgt
    20461 aagggatgtt tttgaaatta cgggtaacgt caagataggc ttcgtccagc gacaagggtt
    20521 cgattaaatc ggtataacgc ctgaatacgg cgtgaatctg cgcggaaacc tgacggtaca
    20581 aatcgaaatg cggcggcaca tacaccgctt gcggacacag ccttttcgcc gttgccaccg
    20641 acatcgcgga atgcagcccg aactgccgtg cctcatacga tgcggcgcaa atcaccgaac
    20701 gcgcgccctc ccacgcgacg accaccggcc gccctttcaa atgcggctgt tcgcgcagct
    20761 ctaccgatgc gtagaatgcg tccatgtcga tgtggataat tttgcgtgaa gacatcggct
    20821 cttctgagga taaaagggat attctactgc cggcatcggg caaattccaa atatacgccc
    20881 cgataggcct gcctccataa aaatgccgtc tgaaacatac cctgtttcag acggcatccg
    20941 caaaactacg gttttcaatt aaaactgcca atccagtttc atgctgacag tgcgcggttc
    21001 tccatagaag ttgtttgcgc cgcgcgtacg gttgtagttg ttctcaaaat aagtgcgtcc
    21061 gtttaagttc gtaccgatga ggctcaattt agcgtgtttg cccaattcgt aacggacgaa
    21121 accgtctatc agcccgtagc cgccctgcct gatgttgtac aggctgcttg tgccgctttg
    21181 tgcggacacg ccgccgccga cggtcagccc cgtattcggt atatggaagc tcgttccgaa
    21241 acggaatatg tgcacgggtg tgaaattgct gaagttgtac gggtctgcgc ctgtgttttt
    21301 ggcgaggcgt tcggcgttga cttcggcggc gtttttgtag cggctcttgt tgtaggtgta
    21361 acccgcaaag actttccaat cttcgttcaa ctcacccgac aactcggcct ccgccccacg
    21421 gctgaccact ttgcctatcg gtttggcaac ggtttggaac gacccctgct tgccgcctgc
    21481 tccgggaaca tagccaaaat cgacgaccgt gcggtttttc tgttcgaggt aaaacaatgc
    21541 gaacgaagca ttcagccgtc cttgcaagaa tgcgcctttc cagcctacct catagtttgt
    21601 gccgaccaaa ggcggcaaaa cggttttggc actgacatcg acattatcct gctgtttgaa
    21661 gattttggta taacttccgt aaatactctg ttgcggtgtc aagtcatagg tgatgcctgc
    21721 atagggcgtc aatttatgac cttgcatctt ggccgtgtaa tggtcctgat ccgccttaat
    21781 actcgatgct gtctgaaaat cgcttgccgg ctgcccatag cggacaggca tatctttggt
    21841 ttgcgaggtt tcatagcgcg tgtagtgcag cccgcccaaa aggtgcagtc ggccggttac
    21901 gttgaaacgc gtgctggcag tcagcgaatg ggttttgttg gtgttgaggt atttggcgta
    21961 gttatacagc gcaggaacat ggtcatcggc cactttaaca gttttccaaa ccggcaccgt
    22021 accggaaaaa ccggtaaaga caggcgagcc gtcgggattg gtctcctgaa tcttgttgcc
    22081 tttttcgtcc agctcatata catcgacata taccggtgtc cggctgccgc tgtattcgtc
    22141 atagtaatac acctgcttgc cttcggcatc gagcttgggc tcggttttta ttttcttggc
    22201 attcctgcat tcttcggcat aaacggtacg gttgcctttt tcatcgtacg cctgccaatc
    22261 gggttcttta tgcccccctg accaaaggag aagacaaatc accgtccggc tcccctgaca
    22321 acttcccgca tacacgccgt gcgttgcccc tgtattcgga cgtactctgt ggcggcgttc
    22381 gtagatttcc agatattcgg aacgtatctt ttcatcgccg taggcatagc cgacaaagaa
    22441 atcatgctcc cgcccgaaca gcccatatgt gccggtcagg tcaagtttaa ttccccattg
    22501 gcggtcgtct ttggtatgcc gcaacggcat ataactgtat cgtcggttgg cggtagcctt
    22561 ccgattaaag gaagagtcat acaggctgtt tggaaaacgt tgtgccgcat tattaaagat
    22621 gccttccttc gcaagggctt tatcgacaaa ttccgacttg tcggcatcaa cacccggatc
    22681 cccccaagaa ccttgacaga taaagtccag cgcaaaaggg gcactcatac actcgtcaaa
    22741 accggctttg cgttctgcgg cacggcggct gcggtactgc tcgaaagcag tattatcgaa
    22801 acggttttta acaaaatcgt ctttgcgctc acggtattcc ttggcggttt cgtcacgata
    22861 tgctttcagt ttctccaatg ccttatcttt cggctcgaac gggatgactt cgtttttttc
    22921 ggtcaaaaag cctaccgcat cctcatctga caaacccgcc gcatgttcgt ttttcagaaa
    22981 aaactgcccc accttcgcat cggattcatt cttggtataa gacacttcgg cattgagctg
    23041 ccaaccgttg tcaaacacat gtttgaaacc tgagaaaagg ttgtatttgt cagcactcaa
    23101 ccgcgaccaa tcctccccca aataagtgtt gcgcggcagt tgcaaaggcc ggttgcaggc
    23161 aggcgttgaa ctgaacgggg cagttttctg attttcacag gacaaaataa tgcccgaaaa
    23221 atcaggaacc tccctactct tctgatacat gccgcccaaa gtaagcacac tgctgtcgcc
    23281 cgcatcggct tcggcaatgc cgtaaaccat atgtttcctg ccccaaacgc ggtctttaaa
    23341 cgatttttta tactcttccg cacccaccaa ccttccgcgt aaggtattcg ccttattcag
    23401 gctgcctgaa acatccaaca ctgcacgccg gctgccgcga tggtcggcgg tcagctctcc
    23461 ggtatgtttg aaagaagcgg taggtcgctt acggatcaaa ttgacggttc cacccggctc
    23521 tgaattggat tgggtcaacc ccgttgcacc ccgtacaact tcaatatggt cataaaccgc
    23581 caaatcggta ctcggagaca cgtcgatttt cgccgtatat cccgaacggc ctgcaacatt
    23641 gacggtaata ccgtcttcac caatctgatc aatatagaaa ccgcgtgaca aaaaccgcgt
    23701 ctgcaagcct gaatcgcgca caacgttgac acccgtcgtg tttttcattg cctcttcaag
    23761 cgtatgcacc gccttatcgt caaggcggct gcgcgtgatg acgctgaccg actgcggcgt
    23821 atccttgccc gcaatcctca tacctgtggc ggtggacatc cgatctatcg tataagaacg
    23881 ggtcttctcg gtcttgcccg acagagcatg agagccgcgt acattgaccg tatccagact
    23941 gacggtattg ccgtctgaaa caggcacaac accgtctgca aaagaaccac cgtaagccga
    24001 taacagcata acggtcagaa ttttaagtga aaaatgattt tgattcatag agacctctgt
    24061 aatatgcaag tgtgcaaatc gtccaaaggc cctcacaact gttttgattt tttatattaa
    24121 ttgaaaaaaa gtaattctca attaaattta tagatgggat tgtattccca ttttgacaaa
    24181 aaacaaactg ctccttaccg tttatttcaa aaaacgataa tattgtattg aaaaatatcc
    24241 gaatttaaat acagatcgtc aatgcggaaa aaaacaccca aattggctat aatcccgaca
    24301 aacacactca aggacaacaa catggcagcc tcgcccgaag caaaattcac tgaagaaaag
    24361 attttgtggg tcaaacacca cacgccgaaa ctcatcactt tcgccatcag ccgtccagaa
    24421 tcctaccgtt ttaaagccgg acagttctcc cgactcggtt tctacgaagg ggaaggcttt
    24481 atttggcgtg cctattccat cgtttccgca gaatatgccg acacgctcga atattttgcc
    24541 gtactcatcc aagacggccc catgtcgatc cgtttcgcca aaatgcaaca gggcgacacc
    24601 atcctgctcg ataaaaatgc caccggcttc ctcctgcccg aacgcttccc cgacggcaag
    24661 gatttggtga tgctctgcac cggctccggc atcgccccct tcctttccat tctcgaacaa
    24721 cccgaaatcc ggcagcgttt cgataccgtc aacctgatac attccgtatc ttttcccgaa
    24781 gaattgattt tcaacgaccg gctcgccgca ttgtccgaac accccctggt aggcgaatac
    24841 ggacactcgt tccgtttcgt ccctgttacc acccgtgccg ccaacccctc gggcttaagc
    24901 ggaaaacgca ttccggaact cttaaaaaac agcagcatcg aacaggcgct gcataccaag
    24961 ttcaccccgg aatccacacg gtttatgatt tgcggcaacc cggaaatggt caaagacact
    25021 ttccaaacgc tgctcgacat gggttacgtc atgcaccgca accgcattcc cggtcaaatc
    25081 atgatggaaa acggcttcta aaaaccaccc tgcttgtccg atgccttcgg atggaagggc
    25141 aaaccgacac ggcacgaaaa ccgcgtcggt aaaaatgccg tctgaaaaaa ttcagacggc
    25201 atcttcggac acattacctg aaacggtaac acattccaac ccaaaccgat caggcaatca
    25261 atacgaacga ctgtttacat acttaccggc tttcaccaac cggtattgat ttaaccgatt
    25321 tccttaatat ttttcctgtc cgttttaaac ttcgccttaa acacatccgg taaatcttta
    25381 tcgaaatacc aaagcccgtc atccatttcc aatgcgccgc ccattccgtg cagaacgact
    25441 gttttccccg tcagcggatc ggtttcggta atttccactt cgtgcatttt gccccaatcc
    25501 aaaacaggca acttgtgtgc aaacgccaaa acctgttcgt cagagcggtc gcacgcctta
    25561 tcgcatcggc ctgaaacata tacgggacaa ggctcatcct ctgcataacc gtccggaagg
    25621 ataccggctg aggcaggaca caatccgtat gtttccgccc acgacgacaa agcccgtctc
    25681 gccgcctttt tattggaaaa taatccggta ggcggattat ccgtcacacc gtttttcaaa
    25741 gccgctgttt tcgcattcaa catgccgtct gaaccttttt caaacctgac ggtcgtaaat
    25801 gttttaagca gatttttggc agatacgtaa caatccaccc cataatgcct gaccaattcc
    25861 gctttaacct tatgggcatg cagcctgccc aatgcgggaa aaaaacggac ttcctccgta
    25921 ttgcaccaat caaacggggc ttttccggag tccaataaag ccgaaacctc gctatatacc
    25981 cgttcaaacg taccgatgta atttactttc cctccgccgt cgaaacaggc cagcaccccc
    26041 ataccgtcag gcaaaccgta caactgttcc ctcaaccgtt cgggcagcgc ggcaggcaac
    26101 ggtttcggat tcatcaaacg gaaacactgc ctgatccatg cctcgacccc gtgttccgac
    26161 agactgtatt ccaaataatc gcacaatgcc gatacatccg ccatcgcacg atgcctgtct
    26221 tccacaacaa tccccaacct ttcgatgata ctgtccaggc tgtgcttgta aaattgcgga
    26281 tacagacacc gggacagttg cacactgcac aaagcaggcg atgaaaatcc gatacccgca
    26341 cgatgaaact catgctttaa aaacgtatag tcgaaacggc tgttatgtgc aaccagcaca
    26401 caacccttca ataccgaaaa caactcgccg gcaatctctg caaaaacagg tgcgtcggca
    26461 accatgccgt ctgaaatccc cgtcagcccc gccacaaact gcggaatcgg tttttgagga
    26521 ttaaccaacc actcatgcct caccaccctt ccctgctcaa acttgaccaa agccacttcg
    26581 gttaccctgt cttcatacag attgccgccc gtcgattcca aatcaaccac ggcaacaggc
    26641 attccaaacc gtaaaaatgc cttttccagc aagggccaac gggaagtaac aatcatttta
    26701 ttctctttaa attcaaacaa caaaccgata ttttacactt ttgaggcatt tcatccaaca
    26761 aaacaattga cagaatccga tgattaccct aaaattcaaa tctttcttgc agcgcacccg
    26821 tagctcagtt ggatagagta tctggctacg aaccagaggg tcgggcgttc gaatcgctcc
    26881 gggtgcgcca gtaagaaaat acaatatgcg cccatcgtct agcggttagg acatcgccct
    26941 ttcacggcgg taaccggggt tcgattcccc gtgggcgtgc caaattccaa atcccccgaa
    27001 ataatccctc ggggattttt tattgcctcg gcaattcatt accatatctt tacctacccc
    27061 cttcatcaga ctctcagacg taatcgaatc atattcaaac ctttgccgtg caaaccgata
    27121 tcacgtaacc ggatgcggtg tccgtccaac attttacccg attgaaacgc ctgatatatt
    27181 gcaccccatc aacgtggcat tacttttctt aacaatcccc tttgacagca actgactagg
    27241 gcttttttat gccatcatca aatttatagt ggattaactt taaaccagta cggcgttgcc
    27301 tcgcctttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
    27361 attcactata atattttctc tcccgattga aacaggcgta acagaatatg cccgaagctc
    27421 cggctgcttt cttgtttacc gccgcgatat ttagagtata ataccaaatt tgagcaatag
    27481 ttctaaaaca gttagaacca tttttcatga gcctgactga ttcgtatact cggagaaact
    27541 gatgcaaaat atttttgacc ctttggttat tcgtggaaaa tcccttaccc ccatcgtgca
    27601 aggcggtatg ggggtcggtg tttccgcatc gggtttatcc agcgcggtgg cacgtgaaaa
    27661 cggtatcgga acgattgcca gtgtggattt gcgccacctt cacgaagacc tactcgccga
    27721 atcacaaatc aatccgagtg aagagaaata tacatctttg aactgtaccg cattagacag
    27781 ggaaatccaa aaagccaaaa gcgcttcaga gggaaaagga ctgattgcgg tcaacgtgat
    27841 gaaggcggtc aaagaccacg ccgcgtatgt ccgccaggct tgcgaatcag gggcggatgc
    27901 ggttgtaatg ggtgccggcc tgcctttaga cctgccggaa atgaccgagg gctatcataa
    27961 agatgtcgcg ctgctgccga ttctgtccga atcgcgcggt attaatatcg tcttgaaacg
    28021 ttggatgaaa aaaggcatat tgcccgatgc gattgtagtc gaacatcctg cccacgcggc
    28081 cgggcatttg ggcgcgtcaa ccgtcgaagg cgtaaacgat gctaaattcg acttcaaacg
    28141 cgtgattgag gaaacgtttg aagttttcaa aagtttaggg ctggaaagcg aaaaaatccc
    28201 gcttattttg gcaggcggta tggcaaactt tgaaaaagtc aaaaccgccc tgaaaaactg
    28261 gggagcatcc gccgttcaaa tcggtacggc ttttgccgtt accgaagaag gagatgcgca
    28321 ccttaacttc aaaaaaacgc ttgctggtgc ggaaactgaa aaagtagtcg aatttatgtc
    28381 tgttgccggt ttgccggcgc gcggcgtacg taccaaattc ctagacagct atatcaggcg
    28441 cgaaggcaaa cttcaggcaa acgccaaagc cgacccgcgc cgctgcaccc aaggattaaa
    28501 ctgcctgacc agctgcggcc tgcgcgacgg gctttccaaa gcagggcaat tctgtattga
    28561 tatccagctt gctgccgcat tccgtggaga agtggataaa ggcctgttct tcagaggtaa
    28621 agaccctctt cctttcggca atgccatccg caccgtccgc gagacgatac aatatctgct
    28681 gacggggagc gaacctgttg caacgctcgg acgctgacat caggtaggat ttgatttgca
    28741 aacaaaatgc cgtctgaagg gccttcagac ggcgtttttc aggctgcgtt tcggaatagt
    28801 gtcgaaaaaa cggagagaaa taaaaacgtt tttatgtaaa caaaacgcct tccgcattgt
    28861 gtcgggcata caacacaaat gccgtctgaa gcccttcaga cggcattttt caggctgcat
    28921 ttcggaatgg tgttaaaaaa taaacgggat aagatacatt tattttcgtc cgataaatca
    28981 gaccatcacg ccgaataatc aaacaatgcc tgcccgacat tgcatctggc aaaccaatgc
    29041 aatgaaaaca cggctttttt atttgcttcc ggcattattg aaaagtttat ccatcggggt
    29101 cagatccacc gcattgcctt ggctggtaat ccatttgttc tcgacgcgcc acacgcctgc
    29161 cgcgtcctcc acgcgcacat accagtggtt ggtcggcgaa agggttttga acaccgcctc
    29221 atattccgcc ctgccgttct gcgcgctgcc gacgggcttg agggcgacgg tttgatcgtc
    29281 cgccttgcgg gtcgggtgca tcagcagcag gttcaaaggc tgtttgccgt caaactcgcc
    29341 gccgacaaac acttttgccg cattcatatc gggggaaatg agaacctgca ccccgatatg
    29401 ccgtctgacg gcttcttcat cccgatgaag ctggatgtcg atatgtttgc cgtctttata
    29461 ataatcgtcc gtaaccaaat ctgtcgcgtg ctgctgcgcg acaaaaaaca tagcgacgct
    29521 ggcgatgacg acaaaaatcg gccccgccat caagatccac ggccagacgt gtttgtacca
    29581 aggtttgatt ggagtgtttt gagacacggt tattctccga taaaggttgc atcttcttcc
    29641 aagacgaccg gcttgccgtc gggcgcgccg ctttcgcggt attggaaggt aaattcgata
    29701 gggtggctgc ctttgtccgc gtattccgga atggtggaca cttggacggg aagggttacc
    29761 gtttcgcgcg gggcaacctt gataccgcct tcgggcagcc cggtcagggc gatttcgtca
    29821 aagcctttga cacttgcggt aatgagctgt tctttttcac ttttgttgat gatacgcagg
    29881 ctgtatgcgt tttccagcca gcctttggcg ttttcgcgca ccagtacgcc acggtctttc
    29941 aaaatatcga cctcgaccat tttgcgcgtg gacaaaccgg ccaggaaggc aatgataact
    30001 aacgccaaca ccgcgccgta acctgccacg cgcggtctga gcagccgttt tttaatgtct
    30061 ttttcagaat attcgtgttc cagcgcgctt tcggtcgtat aacggattaa tccgcgcgga
    30121 tagcccattt tgtccataat ctcatcgcac gcgtcgatac aggcggcgca gccgatacat
    30181 tggtattgca gaccgttgcg gatgtcgatg ccgacggggc agacttggac gcacatcgca
    30241 cagttgatgc agtcgcccaa acccgcctct tccttattga ccgttttctt gcgcgcgccg
    30301 cgcggttcgc cgcgttccgc gtcataagaa acaatcagcg tgtccttgtc gaacatcgcg
    30361 ctttggaaac gtgcatacgg acacatatgc aggcatactt tttcacgcat aatgtgggcg
    30421 aagaagaagg tcataaagcc gtaaaacgct gcggcaaaca tcgcgccgcc acctgctgct
    30481 ccagtgaata aatcgggaac gaactggcgg atagggacaa accagcctgc aaacgtgatg
    30541 cccgtccacg cgcagacaag gaaaatcagc aggtatttgg tggctttgat gcggatttta
    30601 gtgaaattcc acggcgattt ttccagtttc agccgtttgt ttctatcgcc ttcgaccagg
    30661 ttgtcaatcc acagcataat ttcggtgtaa accgtttgcg ggcaggaata gccgcaccac
    30721 agtcgccctg caatcgtcgt ccaccaaaac agcccgaagg cgcaaatcat cagcagcaag
    30781 gcaaggtaaa tcaaatcgcc cacccccaac gacaatccga aaatgaagaa atgccgttcg
    30841 gggatattga aaacgacggc ctgcctgccg ctccagttga accacggaat gacgtaaaac
    30901 acaaactgcg tcgccaatac ggcggcgata cgcagtttgg cgaaccgtcc ttccgccttt
    30961 ttgggatgga tgcgttcgcc ttcgggatgg atttgaatca cgctgactcg cggatcgaat
    31021 gttttttttg ctttcggcgc ggcttttgtt tgttcggacg tgccgattcc ggatgccgga
    31081 ctgccggctt ggttttccgt ggtcattctg cattccttag atttttgatt gatggtttgc
    31141 ccgttaccgc cgccgtttgc ttttcagacg tcatttttct tgttttttaa ggcgttgtgt
    31201 ttcaagtttt gagaaaatcc gtttttccca aaatatattt ccgctattgt acaactttat
    31261 gcgccgtccg gatgtatggg gcggatacat ttcccatccg catcaaaacg cctggatttt
    31321 accttactgc ccgaacaaaa tccgaatacg gttaaaaaaa aagactaaaa aaaccgacac
    31381 ccccatatcg gcagaaccga cggtgcaagc tcataaacaa acgctatcga caatccggca
    31441 cacaatctat aactttttat ttcaaaagga ataatggcag gcttcgcccg caaatcgaaa
    31501 atccttcccc accggtctcc tgctgccgcc ttcccccgcg tccgcccttt tcttgaaagc
    31561 ataagcgaat cgggcgataa tcaacgcttt ccgattatcc acttatccga aacaccagca
    31621 aggaaaatac aaaatgtctc aactggcaaa cgcaatccgc ttcctctcgg ccgatgccgt
    31681 tcaaaaagcc aattccggcc accccggcgc gcctatgggt atggcggaaa tggcggaaac
    31741 attgtggacg aaattcctca atcacaatcc cgccaacccc aaattctaca accgcgaccg
    31801 cttcgtcctc tccaacggcc acgcgtctat gctgttgtac agcctgctgc acctgaccgg
    31861 ctacaatctg agcattgaag acttgaaaaa cttccgccaa ctgcacagca aaacccccgg
    31921 ccatcccgaa tacggctata ccgacggcgt ggaaaccacg accggcccgt tggggcaagg
    31981 gattgccaac gcggtgggca tggcattagc cgaaaaaatc cttgccgccg aatttaataa
    32041 agatggtttg aacatcgtcg atcattacac ctacgtcttc atgggcgacg gctgtctgat
    32101 ggaaggcgta tcgcacgaag cctgttcgct cgccggcact ttaggcttgg gcaaactgat
    32161 tgttttgtat gacgataaca atatttccat tgatggtaaa gtggacggct ggtttaccga
    32221 aaacatcccg caacgctttg aaagctacgg ctggcacgtc gttcctaacg taaacggtca
    32281 tgacaccgcc gccattcaag ccgccatcga agccgcacgt gccgaaaccg gcaaaccgtc
    32341 catcatctgc tgcaaaacct taatcggcaa aggcagtgcc aacaaagaag gcagccacaa
    32401 aacccacggc gcacctttgg gcgcggacga aatcgaagcc acgcgcaaac atttgggctg
    32461 gacttacccc gcctttgaaa tcccgcaaga aatttacgat gcgtggaatg ccaaagaaca
    32521 aggcgcgaaa ctggaagccg actggaacga actgttcgcg caatatcaag ccaaatatcc
    32581 tgccgaagcc gcagaatttg tgcgccgtat ggataaaaag ctgccggaca atttcgatga
    32641 atacgttcag acggcattga aagaagtgtg cgccaaagcc gaaaccatcg ctacccgcaa
    32701 agccagccaa aacagcatcg aaatcttggc aaaagagttg cctgaattgg tgggcggttc
    32761 ggccgacctg accccgtcca acctgaccga ctggtcaaac agcgtctccg ttacccgcga
    32821 caaaggcggc aactacatcc actacggcgt gcgcgagttc ggtatgggtg cgattatgaa
    32881 cggtcttgcc ctgcacggcg gcatcaaacc attcggtgcg accttcctga tgttcagcga
    32941 atacgagcgc aacgccctgc gtatggcggc actgatgaaa atcaaccctg tatttgtgtt
    33001 cacccacgat tccatcggtt tgggcgaaga cggtccgacc caccaaccca tcgagcaaac
    33061 cgccaccctg cgcctgattc cgaatatgga cgtatggcgt ccgtgcgaca ccgccgaatc
    33121 tctggtggct tgggcagaag ccgtcaaagc cgccgatcat ccgtcctgcc tgattttcag
    33181 ccgtcaaaac ctgaaattcc aagcgcgcag cgaacaacaa ctgaacgaca tcaaacgcgg
    33241 cggctacgtc atcagcgaag cccaaggcaa cgcccaagcc gtcatcattg ccaccggctc
    33301 agaagtcgag ctggctttgg aagcgcaaaa agcactcgcc gcgcaaaaca tcgccgtacg
    33361 cgtcgtttcc atgccgtcca ccaacgtatt cgaccgccaa gacgccgcct atcaagccgc
    33421 cgtcctgccc gaaagcctgc cgcgcatcgc cgtagaagcc ggccacgccg acggctggta
    33481 caaatatgtc ggactgaacg gcgcagtcgt cggcatcaac cgcttcggcg aatccgcccc
    33541 tgccgattta ctcttcaaag cattcggctt taccgtggac aatgtggttg atacggtgaa
    33601 atccgtgctg taaccccaca cctaaacaaa tgccgtctga acgcttttca gacggcattt
    33661 ttttgccctc ccgccttgat gcttcctcat tccgccaatc tccattataa tatttgcgaa
    33721 tcactcttat tcacatttca aaaggagaaa cgcatgagca cccgtaccga acatgacacc
    33781 atgggcaatg tcgaagtccc gtccgaagcc tattggggcg cgcagaccca gcgcagccgc
    33841 aacaatttca aaatcggcgg cgaaaccctg ccgcagccgc tgatttatgc tttggcgctg
    33901 gtgaaaaaag ccgccgccgc gaccaatgtt tccctcggca ggattaagcc ggaacaggcg
    33961 gatttgatta cgcaggcggc ggatgatgtg ttgaacggca ggcttgacgg acagtttcct
    34021 ttagtggttt ggcagaccgg ctccggcacg cagtccaata tgaacatgaa cgaagtgctg
    34081 gcaaaccgcg ccaacgaaat cgccggtacg ggtttggcgg cttatcagcc cgtccatccc
    34141 aacgaccatg tgaaccacgc gcaatcgacc aacgacgcat tcccgaccgc tatccacgtt
    34201 gccgccgcga ttgaaatcaa ccgccacctc atccccgccg taaaagccct gcgcgacacg
    34261 ttggacaaaa aagcccaagc tttcgcccct atcgtcaaaa tcggccgcac ccacttgcaa
    34321 gacgcgacgc cgctgacttt gggacaggaa ttttccggct acgtttccca gcttgatcac
    34381 ggtttaggcc gtctgaacga tgcgcttaaa gacttgtatg aacttgcttt gggcggtacg
    34441 gcggtcggca cgggtttgaa cagccatccc gaatacgccg aaaaagctgc tgctaaactc
    34501 gccgaactgt ctggcttgcc gtttgtcagc gcgccgaaca aatttgaagc cttaggcgga
    34561 cgcgatgccg ccgttgccgc ttcgggcgca ttgaaaacgc tggcggcaag cctgaataaa
    34621 atcgccaacg acatccgctg gctggcaagc ggcccgcgtt gcggtttggg cgaaatcaaa
    34681 atccccgaaa acgagccggg ttcgtccatc atgccgggca aagtcaaccc gacccaatgc
    34741 gaagcaatga cgatggtgtg ctgccaagtg ttcggcaacg acgttaccat cggtatggcg
    34801 ggcgcgtcgg gcaatttcga gctgaacgtc tatatgcccg ttatcgccta caacctcttg
    34861 caatccatcc gcctcttggg cgacgcgtgc aacagcttca acgaacactg cgccgtcggc
    34921 atcgaacccg taccggaaaa aatcgactat ttcctgcacc attccctgat gctcgttacc
    34981 gcgttgaacc gcaaaatcgg ttacgaaaac gccgccaaag tcgccaaaac cgcctacaaa
    35041 aacaacaaat cgttgcgcga aaccgccgtt gagctgggct tgctgacggg cgaagaattt
    35101 gacgaactgg tcgttcctgc cgatatggtt catccgcgct aatctttccc tcaaataaaa
    35161 tgccgtctga aacctcgttc agacggcatt ttccgttgcc tgcgaactag cggcgtttga
    35221 acagcctgtc ccccaccgcc gccgtaaccg cacccccgac cacgaccagt gcgccaacat
    35281 aacccaaacc gttcatatcc ggcgcggcaa aagtatcagg catcacataa tgcccgagca
    35341 aagaaaatat tacggtaaac acggggagca aggttgttac cgcgctgact ttggaagcct
    35401 cccaatgttt caacgcctcg ccgaacgagc cgtaaccgat taacgtattc aagcagcaat
    35461 acgcaaaaca aacccacgcc aacgtaccgt ccaaacttcc gatgtgtgcc ggttcggcaa
    35521 acggcaggaa cacggcggca cttgccgcat aaatcaacag cagaatctgt tgcggcccga
    35581 attgcgccga cagcagcttt tgcgccacgg cataacacac ccatgccata ctgcctgccg
    35641 cacacagcaa cacgcccttc gcatacgcgc ccaaacccga caactcgccg aatttatcgt
    35701 taaaaaacat aagcaaaccg gcaagcagca aaaccaagcc gattttctga gcggcagtca
    35761 tccggtcttt aaacaccaac acaccgacaa caatcatcgt aaacggcgaa atctgccaca
    35821 aaacctgcgt cgtggtcggc gaaatataat gcagcccttg ggcaatcagc acaaagtttg
    35881 ccgaaatgcc cgccacgccg agcagcagca gcctgaatga gcaccaagaa aaatcccgcc
    35941 gcttcggcag ccgcccgccc agtgccagca aaacaaacaa taccgccgcc gccacggtaa
    36001 aacgcaccca caccagcgtc ggcgcatcga caaacttcaa tacctgccgc acggcaatcg
    36061 gcagcgttcc ccacgtcatc gccgccaaaa gtgccaacgc gaagcctagg tgcggccttt
    36121 ggttttccat cctgattttc ctatttttaa acaaccgtat tgccggacga tgccggtttg
    36181 ccgcatcggg caataatggt tcaagcgttt ggcgtttaat tccaaccctt tgatttcaaa
    36241 caaaccggct gaagctcggc tattgcttcg cgctatttga aaacaccgcc tgaattttaa
    36301 aatatagtgg attaacaaaa accagtacag cgttgcctcg ccttagctca aagagaacga
    36361 ttctctaagg tgctcaagca ccaagtgaat cggttccgta ctatttgtac tgtctgcggc
    36421 ttcgtcgcct tgtcctgatt tttgttaatc cactataccg tctgaaaaca gcggggacgt
    36481 gcggcaggca tcactgcctc aaaacgcccg aacggcgcaa tcgcaacgtt ccgcccaacg
    36541 caaagggcgg caacaagccg gcccaaatgc aaaaaagaga aaccctgccc cgtaaggttt
    36601 aaggtttctc cgtcctttat gatttccctc tgcgaggatg tccggccgta aaattcagaa
    36661 cgggatgtcg tcgtcaatat cctcgaccgg ggcggcggca ggcacgggtt ggcggcgcgg
    36721 cgcggctggt gcttcttggg gatgggacgg cgcgtcggag gcgggctgcc ggctttgctg
    36781 tgcggggcgt tggtaagcct cctgactctg accgtaacct tcctcgtaag gcgcaccgcc
    36841 gctgttttca ttgcgcccgc ccaacatttt catttcgttg gcgacaatat cgtaagcggt
    36901 gcgttcgatg ccgtctttgc cttggtattt gcggctttgg attctgcctt ccagataaac
    36961 cagcccgcct tttttgaggt attgcccggc aatttccgcc agtttgcggt acatcgtgat
    37021 gttgtgccac tcagtacgct ctacacgttg gccgttgcgg tcgttccaag tttcgctggt
    37081 ggcgacgctg aaattacaca ccgcctcgcc gttgggcata tagcgcactt cgggatcgcg
    37141 tccgaggcgg ccgataagga taactttgtt caatgacatt tttaaactcc tgtaatgatt
    37201 ttttcagcgg cagcctgatc gaaacccttc tgcaacactt tgagatagac ggtctgcccg
    37261 tcgaaactga aaccgatgtc ttccacgcct tcgagttgca acagggcgcg ttgcaatccg
    37321 tcccgattga cctgccacac gccgccgaca gggtaactga ggtttttgac gggcttgggc
    37381 gcaggcgata aaacggcaat caccagccac agcagcatca atatgctgca aaaggcaaac
    37441 acgccggaaa agccgtattt ttgaaacagc aaaccgcctg ccacgccgcc ggcaaacagt
    37501 ccgagcgact gcatcgtgtt gtacacgccc atcgccgtac ccttcaggtc ggacggcgcg
    37561 attttggaaa ccatagacgg caggctcgct tccaacacat taaaaccgat aaagtaaaca
    37621 accaaataag cggtaatcaa gcctaccgag cgcataccgg acagcaaacc gagctgtgcc
    37681 gccgcgatgc aggcgatgcc caagataaaa acctgtttga gcttgttgcg cgtctcgccg
    37741 acgataatca gcggaaccat caccaccaag cccgtaatgg tcgagggcag atagactttc
    37801 caatgctgga ttttttccaa accgagctgc gtcatcgcaa aaggcagcgc ggtaaacaat
    37861 gccatttgcg cggcgtgcag ggcgaaaatg ccgaaatcga gcgtcagcag cctacggttt
    37921 ttcaaaactt cgcctatgcg cgaaggctgc gcctgcgtat cttcgtgcag cttggaaact
    37981 tcgggatcgg gagtcatcca cgccaccacg ccgatgctga tgacggtcag aatgccggtc
    38041 agcataaaca gtccgcgaac gccgaccgcg tcggcaatca cgggggcaac gacgaggctg
    38101 accgaaaacg tcaaaccgat actcaaaccg atcatcgcca ttgcgcgggt acgtacgccg
    38161 tcgcgcgtca aatccgccag cagcgcggta accgccgcac tgaccgcccc tgcaccctgt
    38221 atggcgcgtg cggcgaccag catgggcagc gtatcggcgg cggcggcaag aaagctgccc
    38281 gccgcaaaca cgaccagtcc cgcataaatg gttttcttgc gcccgaactt gtcggaagcg
    38341 atgcccaaag gcagttgcag cagagcctgt gtcagcccgt aaatgcccat tgccagcccg
    38401 accagcgttt tgttgccttc cgcgccgggc agcgaggcgg catacaccgc caatacgggc
    38461 agcacgagga acatacccag catacgcagc gcgtacacgc cggaaagcgt cgtactggcg
    38521 cgccattcgt gcggaaacat ttggatgcgg ttgtctcttg ccatcatatt ttttcagacg
    38581 gcatcaacag ttgcaatgcc gtctgaactt ccagtgaaca gattttcgga ttatacagga
    38641 ttcgccgtat ttcggttgcg gcgcgggttc aaaattaacg ccactgccag cggttgcgcc
    38701 acgcgcccaa aacggcgttc ggatatttat tgctgcccaa gctgccatta aagcgggcaa
    38761 gcgcgcggac gatgttgcct ttttcaagat tccggtaatg gcgcaggatg gtacagccgt
    38821 aacgcaggtt ggtgcggatg tcgaacaggt tgtgcgccgg tttgccgatg tagtttttcc
    38881 aaaacggcat aacctgcatc aggccgcgcg cgccgacacc gctgattgca tactggcgga
    38941 acgcgctttc cacctcaatc agccccaaca caatctgcgt atccaaaccg gcccggctgc
    39001 tttcatactg aatattgacc agcagcctgc gccgctcctc ctcctcgggg acgaaccttg
    39061 ccaaacgtgc cgacatggca gacaaccaac gttcgccctc tttcggattg tcaaacacca
    39121 gcctcggcgg attgacgctg ccgacagaac tcctcatcac ggaagccaca tcgtcggcaa
    39181 gcgtttcctc acgttgcgcg ccggcgtgcg ccagaggact gagcaacaac gcaccggcgg
    39241 cacacaacag gcggcggcgt tgcagattaa cgggtagggt atcggtcggt tttttcatag
    39301 ggaacggggg cgcgtccgga cgtttcagac ggcattaaat attcaaacag acaatgattg
    39361 cttccaacgc gaaaaaccgc gcgcaaaatc caagcgcggc atatcgccct gcccttttcg
    39421 ggcaaacctc aattctaccg ccctcaagaa cgcttgtcca aacaggcaca ggcaacaccg
    39481 cccgggcatt tccgttttca ccggttatcc gtcgtccgga ttatgcagca gcaccatcag
    39541 cgcatcacgc ttttcgggcg gcagcaggcg gagacctttg caaaaatagt ctgttaacga
    39601 aatttgacgc ataaaaatgc gccaaaaaat tttcaattgc ctaaaacctt cctaatattg
    39661 agcaaaaagt aggaaaaatc agaaaagttt tgcattttga aaatgagatt gagcataaaa
    39721 ttttagtaac ctatgttatt gcaaaggtct caggcggaaa tatagtagat taaatttaaa
    39781 ccagtacagc gttgcctcgc cttgccgtac tgatttaaat ttaatccact atatcttgag
    39841 gcctttgcaa aattcctttc cctcccgaca gccgaaaccc aaacacaggt tttcggctgt
    39901 tttcgcccca aataccgcct aattctaccc aaataccccc ttaatcctcc acggataccc
    39961 gataatcaga catccggtcg ccttttaggt gccaacaggc gcacttagcc tgttggtcgc
    40021 tttcaacagg ttcaaacaca tcgccttcag atggctttgc gcactcactt taatcagtcc
    40081 gaaataggct gcccgggcgt agcggaattt gcggtgcagc gtaccgaagc tctgttcgac
    40141 cacatatagt ggattaaatt taaaccagta cggcgttgcc tcgccttagc tcaaagagaa
    40201 cgattctcta aggtgctcaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc
    40261 ggcttcgtcg ccttgtcctg atttaaattt aatccactat aacgggtttt cgacaaatat
    40321 cggttacgtt tggtttgcac ttccgccagc ggacgattgc ggcaggcttt gcgcataatg
    40381 ccgtcctgca gccgatgctc tttcagtttt ccgtaggtcg gattctcgaa tccgacatta
    40441 cttcaatcgt atccaataga aaagtccgca ttgccgccac cccaattatg cggataaata
    40501 ccctgtttga cataacggtg aaacgtagaa aacccccaat cggaaatttg tcctacatag
    40561 ccatgtttga ccggattgaa atgcagataa tcaaaatacc aggcaaaatc ggcctcatcg
    40621 cggatagtat attcccaaaa gcgtttttgc caaagcctga gattgccgcc gattaaatat
    40681 tggctgtgcc gcttgatttg ccgccagcgt tccgaataag cagaatcatt gtccggcagc
    40741 cgccatatgg tatgcagatg gtcgggcatc aacacccatg ccaaaatttc aaacggatac
    40801 cgttcgcgca ccgccattac cgcctgccgc aaagccaaac gcaccgcatc atcggtcaaa
    40861 atcttctgcc gtttattggt tacaaccgta aaaaagtaag tgccgccatt gcggt
//
LOCUS       DAXIHS010000005        40295 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXIHS010000005 DAXIHS010000000
VERSION     DAXIHS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.005"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(71..2677)
                     /gene="acnD"
                     /locus_tag="ACFOF2_000200"
     CDS             complement(71..2677)
                     /gene="acnD"
                     /locus_tag="ACFOF2_000200"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC7106589.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKTQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2755..3543)
                     /locus_tag="ACFOF2_000201"
     CDS             complement(2755..3543)
                     /locus_tag="ACFOF2_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC7106590.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIISMF"
     gene            complement(3704..4858)
                     /gene="prpC"
                     /locus_tag="ACFOF2_000202"
     CDS             complement(3704..4858)
                     /gene="prpC"
                     /locus_tag="ACFOF2_000202"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC7106591.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(4944..5819)
                     /gene="prpB"
                     /locus_tag="ACFOF2_000203"
     CDS             complement(4944..5819)
                     /gene="prpB"
                     /locus_tag="ACFOF2_000203"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC7106592.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6445..7755)
                     /locus_tag="ACFOF2_000204"
     CDS             complement(6445..7755)
                     /locus_tag="ACFOF2_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC7106593.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(7894..9072)
                     /gene="ftsZ"
                     /locus_tag="ACFOF2_000205"
     CDS             complement(7894..9072)
                     /gene="ftsZ"
                     /locus_tag="ACFOF2_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC7106594.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9191..10435)
                     /gene="ftsA"
                     /locus_tag="ACFOF2_000206"
     CDS             complement(9191..10435)
                     /gene="ftsA"
                     /locus_tag="ACFOF2_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC7106595.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10521..11249)
                     /locus_tag="ACFOF2_000207"
     CDS             complement(10521..11249)
                     /locus_tag="ACFOF2_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC7106596.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11239..12153)
                     /locus_tag="ACFOF2_000208"
     CDS             complement(11239..12153)
                     /locus_tag="ACFOF2_000208"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC7106597.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12267..13676)
                     /gene="murC"
                     /locus_tag="ACFOF2_000209"
     CDS             complement(12267..13676)
                     /gene="murC"
                     /locus_tag="ACFOF2_000209"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC7106598.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(13831..14898)
                     /gene="murG"
                     /locus_tag="ACFOF2_000210"
     CDS             complement(13831..14898)
                     /gene="murG"
                     /locus_tag="ACFOF2_000210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC7106599.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(14902..16227)
                     /locus_tag="ACFOF2_000211"
     CDS             complement(14902..16227)
                     /locus_tag="ACFOF2_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC7106600.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16353..17690)
                     /gene="murD"
                     /locus_tag="ACFOF2_000212"
     CDS             complement(16353..17690)
                     /gene="murD"
                     /locus_tag="ACFOF2_000212"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC7106601.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLSPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(17780..18052)
                     /locus_tag="ACFOF2_000213"
     CDS             complement(17780..18052)
                     /locus_tag="ACFOF2_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC7106602.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(18167..19249)
                     /gene="mraY"
                     /locus_tag="ACFOF2_000214"
     CDS             complement(18167..19249)
                     /gene="mraY"
                     /locus_tag="ACFOF2_000214"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC7106603.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19496..19672)
                     /locus_tag="ACFOF2_000215"
     CDS             complement(19496..19672)
                     /locus_tag="ACFOF2_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106604.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(19675..21033)
                     /gene="murF"
                     /locus_tag="ACFOF2_000216"
     CDS             complement(19675..21033)
                     /gene="murF"
                     /locus_tag="ACFOF2_000216"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC7106605.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMMAKAWRENVDPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21158..22804)
                     /locus_tag="ACFOF2_000217"
     CDS             complement(21158..22804)
                     /locus_tag="ACFOF2_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC7106606.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYVAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(22827..24305)
                     /locus_tag="ACFOF2_000218"
     CDS             complement(22827..24305)
                     /locus_tag="ACFOF2_000218"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC7106607.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24330..26075)
                     /locus_tag="ACFOF2_000219"
     CDS             complement(24330..26075)
                     /locus_tag="ACFOF2_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC7106608.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(26136..26399)
                     /gene="ftsL"
                     /locus_tag="ACFOF2_000220"
     CDS             complement(26136..26399)
                     /gene="ftsL"
                     /locus_tag="ACFOF2_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC7106609.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(26396..27382)
                     /gene="rsmH"
                     /locus_tag="ACFOF2_000221"
     CDS             complement(26396..27382)
                     /gene="rsmH"
                     /locus_tag="ACFOF2_000221"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC7106610.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(27379..27834)
                     /gene="mraZ"
                     /locus_tag="ACFOF2_000222"
     CDS             complement(27379..27834)
                     /gene="mraZ"
                     /locus_tag="ACFOF2_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC7106611.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            28140..29330
                     /locus_tag="ACFOF2_000223"
     CDS             28140..29330
                     /locus_tag="ACFOF2_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7106612.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQSHFLLNL
                     GITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            29462..29538
                     /locus_tag="ACFOF2_000224"
     tRNA            29462..29538
                     /locus_tag="ACFOF2_000224"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29496..29498,aa:Met,seq:cat)
     gene            29546..29621
                     /locus_tag="ACFOF2_000225"
     tRNA            29546..29621
                     /locus_tag="ACFOF2_000225"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29579..29581,aa:Ala,seq:ggc)
     gene            complement(29903..30724)
                     /locus_tag="ACFOF2_000226"
     CDS             complement(29903..30724)
                     /locus_tag="ACFOF2_000226"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC7106613.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(30757..31401)
                     /locus_tag="ACFOF2_000227"
     CDS             complement(30757..31401)
                     /locus_tag="ACFOF2_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC7106614.1"
                     /translation="MKLKHLLPLLLSAALSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(31404..32267)
                     /gene="ftsN"
                     /locus_tag="ACFOF2_000228"
     CDS             complement(31404..32267)
                     /gene="ftsN"
                     /locus_tag="ACFOF2_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC7106615.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(32373..33869)
                     /locus_tag="ACFOF2_000229"
     CDS             complement(32373..33869)
                     /locus_tag="ACFOF2_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC7106616.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(33883..34203)
                     /locus_tag="ACFOF2_000230"
     CDS             complement(33883..34203)
                     /locus_tag="ACFOF2_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC7106617.1"
                     /translation="MFGKQLFEEVGSKISETIVNSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34687..36213
                     /gene="putP"
                     /locus_tag="ACFOF2_000231"
     CDS             34687..36213
                     /gene="putP"
                     /locus_tag="ACFOF2_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC7106618.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIVYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            36464..40069
                     /gene="putA"
                     /locus_tag="ACFOF2_000232"
     CDS             36464..40069
                     /gene="putA"
                     /locus_tag="ACFOF2_000232"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC7106619.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         9532 a        11398 c        10009 g         9356 t
ORIGIN      
        1 tatcgagaat tacgtgaatg agcaaaatgt tgaacccgac ccacgctacg tcttgctttt
       61 cagacgacct ctaagccgcg ttcccttcca aaaaatcctg tgcaaaccgt tgcaacacgc
      121 cgccggcttc atataccaat acttcttctg cagtatcgag gcggcaggta acgggaactt
      181 cgacggtttc tccgttttta cggtgaatca cgagggtcag gtcgcagcgc ggtgtgcgtt
      241 cgccgaccac gtcgtaggtt tccgtaccgt ccagttgcag ggtatggcgg ttggtgtcgg
      301 gtttgaactg cagcggcaac acgcccatgc cgataaggtt agtgcggtgg atacgctcga
      361 agccttcggc aacaatcgct tctacgccgg cgaggcgtac gccttttgca gcccagtcgc
      421 ggcttgagcc ttgaccatag tccgcaccgg caatgatgat gagcggctgt ttgcggttca
      481 tataggtttc gatggcttcc cacatgcgca tggtttcgcc ttcgggttcg acgcgggcga
      541 acgagccttg gcgcacgctg ccgtcttcgt ttttcaccat ttcgttaaac agtttcggat
      601 tggcgaaggt agcgcgttgg gcggtcaagt ggtcgccgcg gtgggttgcg taagagttga
      661 agtcttcttc aggcaaaccc attttcgcca aatactcgcc tgcggcactg acggccaaaa
      721 tcgcattgga cggcgagagg tggtcggtgg tgatgttgtc gggcaaaatc gccagcggac
      781 gcatacctct taatgtgcgt tcccctgcca gtgcgccttc ccagtaaggt gggcggcgga
      841 tataggtgga catcggacgc cagtcgtaca gcggactagg tgctttttgc gctgtgccgg
      901 tgtcgaacat cggtacatac acatcgcgga actgctgcgg tttcacatat tcggcaacga
      961 cggcatcgat ttcttcatcg gcaggccaaa tgtctttcag gcggatttcc ttgccgtctg
     1021 caacgccgag tacgtcgttt tcaatatcga aacggatact gcctgccagc gcgtaggcaa
     1081 cgaccaacgg aggcgaagcg aggaaagcct gtttcgcata cggatggaca cggccgtcga
     1141 agttgcggtt gcctgacagt acggcggtgg cgtacaaatc gcggtcgatg atttctttct
     1201 gggttttcgg atccagcgcg ccactcatgc cgttacaggt ggtgcaggcg aaggcgacga
     1261 taccgaagcc gagtttttcc atttcgggca gcaggcctgc ttctttcaaa tagatttcgg
     1321 ctacttttga acccggggca aatgaagatt tcacccaagg tttgcgtttc aagccgaggc
     1381 ggttggcatt gcgtgccaac agcgcggcgg caacgacgtt gcgcgggttg gaagtgttgg
     1441 tgcaactggt aatcgcggcg atgatgaccg cgccgtcggg catttgtccg tccgaaggct
     1501 cttcgtaagg cttcgccagc cctttcgccg ccaaatcggc ggtcgcaaaa cgggcatgcg
     1561 ggttacttgg acctgccata ttgcgcgtta cgctgctcaa atcaaatttc aaaacgcgag
     1621 gataaacggc ggttttcaag gcatctgccc ataagcctgc ggttttggcg taggtttcca
     1681 ccaatttcac ctgcgcgtcg tcgcgtccgg tcagtttcaa ataatcaatg gtttgctcat
     1741 caatagcgaa catcgcggca gtcgcgccga actccggcgt catgttggaa atggtcgcgc
     1801 ggtcgccgat agacaggctt ctcgcgccct cgccgaagaa ttcgacaaac gccccgacca
     1861 cgcgctcttt gcgcagaaac tcggtcagcg ccaacacaat atccgtcgcc gtaatgcccg
     1921 cctgccgttt gccggtcagc tccacaccga caatatcggg caggcgcatc atggacgcgc
     1981 gtcccagcat tacggtttcc gcttccaatc cgcccacacc cacggaaatc acgcccagcg
     2041 catcgacgtg cggcgtgtgc gaatccgtgc cgacgcaggt atcggggaac gccacgccgt
     2101 ttttaacttg aacaaccggc gacatttttt ccagattgat ttggtgcatg atgccgttgc
     2161 ccgccggaat cacgtccaca ttttcaaatg cggtttttgt ccagttgata aagtggaaac
     2221 ggtcttcgtt acgtctgtct tcgatttcgc ggtttttgcg gaaggcatca ggatcgtaac
     2281 cgccgcattc aacggccaaa gagtggtcga caatgagctg ggtttgcacc accggattca
     2341 ctttggcagg atcgccgcct ttttcggcaa tcgcatcgcg cagacctgcc aaatccacca
     2401 acgcggtctg ccccagaata tcgtggcaca ccacccgcgc cggataccaa ggaaagtcga
     2461 tttcctgttt tccctcaatc agctgaccca gccagctttg cagcgtcggc aaatcgactt
     2521 tgtccgcgcg gttgaccaaa ttctccgcca aaatgcggct cgtgtaaggc agcttgtcgt
     2581 aagagccggg cttgatgtcc tcacacgccg cacgcgcgtc gtagtattcc aaatccgtac
     2641 cgggcagcgg tttgcggtaa cgttggttgg cagccatgtc ggttctcctg gggatctgtt
     2701 tttcttgtgg tttgaggttt cagccgacgt tttgaagggg tcgtctgaaa gggtttaaaa
     2761 catcgaaata atcatctgat acagcggatt tctctcgtct gtcaacaatt tcacagccat
     2821 cgaaatgctg atgacaatca gcagcggctt aatcagcttc gaaccgaagc ggacggcaaa
     2881 tctcgcacct aaattcgcac cgacaaacgc accgaccgcc atcgttgccg caatcgggaa
     2941 aataatcgaa ccgtgcagca ggaataccga tagcgaacca agattgcagg caacgttcgc
     3001 caatttggtg taagacatcg cgttcaacag cttgcagccg agcaaaacaa taaaggcaat
     3061 cagaaaaaac gagccgacac ccggtccgaa cacaccgtcg taaaaaccca aaagtggtgc
     3121 aaccgtcaga ccgaacagaa aaaaagacat tctggctttg ccttccttac tgccgtcgag
     3181 cttgggcgaa aacacaaaat acagcgcgac aaatatcaac aaaaccggca cgaccgccag
     3241 cagaatatct ttggaaacca agctgaccga taatgcaccg accacgccgc ctgcaaacga
     3301 tgctgccgca atcgggagac ctttcttcca atcaatcaaa cctttgcgtg caaaagaaac
     3361 cgtagccgaa aacgtagcag cggctgcttg cagcttgttg gtggcaattg ccgacacggg
     3421 aggaatacct gccaacaaga gtgcaggcag cgtaatcaaa ccacccccac ccgcaatcgc
     3481 atcgataaat ccggcaatca tcgcaaccaa acccaaagcg agtattatgt ataaatcttc
     3541 catgtttctt atcctgttac ttgcaccaat acaggattat cttcttctat taggttaaat
     3601 ttttaaactt atttcagaca acctttccaa tcaggcaagg tcgtctgaaa tcctcagctt
     3661 tgcataccga aattaaagaa caaactgcta ttgcattctt caattatcgt tcttcaatct
     3721 ccacaaacgc caaatcttca gggcctgtgt agtttgcgct cggacggatg attttgccgt
     3781 ctttgcgttg ctcaagaacg tgtgcgctcc aaccggttgt acgggaaatt acgaacagcg
     3841 gtgtgaacat agcggtcggt acgcccaatt tttggtagga aacggcagag aaccagtcca
     3901 gattcgggaa catttttttc tcttcccaca tcacgctttc caaacgttcg gcaatgtcaa
     3961 agaggcgcat atcgccggtt tctttgctca aaccgcgtgc cacttcttta atgacaacgt
     4021 tgcgagggtc ggaaatggtg tacaccggat gaccgaaacc gatcacgatt tctttgcggc
     4081 cgatgcgttc gcggatgtcg gcttcagctt cgtcggcatt gcggtagcgt ttttgaatat
     4141 cgtaagccac ttcgttcgcg ccgccgtgtt tcggaccttt caacgcgccg attgctccgg
     4201 taatgctgga gtacatatca gagcctgtac cggcgatcac gcgggcggta aaggtagaag
     4261 cgttgaactc gtgttcggca tacagaatca gtgaaacgtg catggctttg atgtgtgatt
     4321 cgcttgggcg tttgccgtgc aacagttgca ggaaatgacc gccgatggtc tcttcgtcgc
     4381 tttcaacctc aatgcgtttg ccgttgtgcg aatattgata ccagtacaag aggatgctgc
     4441 cgaggctggc gatcagtttg tcggcgatgt cgcgcgcttc actttccgga tggctttcac
     4501 gttcaggatg aacgcagccc agcatggata cgccggtacg cattacgtcc atcggatggg
     4561 tatgtgcagg caggctttcc aaaactttaa tcacacggat aggcaggccg cgcatggatt
     4621 tgagcttggt tttataagcg gccagctcga atttgttggg cagatggccg tgaatcagca
     4681 ggtgggcgac ttcttcaaac tcgcattttt gtgccaaatc cagaatgtcg taaccgcgat
     4741 agctcaaatc gttgccggta cggccaacgg tacacaaagc ggtattaccg gccgcaacgc
     4801 cagaaagcgc aacggatttt ttaggtttga gggtcggggt ttgagtagtt tcagtcatgg
     4861 tatttctcct ttgtgttttt atgggtttcg ggttttcaga cgaccgatgc ggatttgttg
     4921 aaaggcagtc tgaaagcggt aaatcatttt tgaaacaatt tatccagttt ttgctcgaag
     4981 gcatgatagt tcagatgctc gtacagctcg gcacgggttt gcatactgtc caccaccgcc
     5041 gcctgagtgc catcgcgcat aatcgcttcg taaacattca gagcggcttt gcttgctgca
     5101 cggaacgatg acagcggata cagcaccagc gacacgccgt tttcagccag ctcgctttgg
     5161 gtataaagcg gagtggaacc aaactcggta atgttcgcca acacgggcac tttcaccgca
     5221 tctgcaaatt ggcggtacat gttcaaatcg gtcatggctt cagggaaaat catgtccgca
     5281 ccggcttcga cacaagcttg ggcgcgttcg atagcggcat ccaaaccttc taccgccagc
     5341 gcatcggtac gcgccataat cacgaagttc tcatcaacgc gcgcatctac ggcagctttg
     5401 atacggtcga ccatttcatc tttagataca atggctttgt tcggacggtg gccgcagcgt
     5461 ttttgcgcta cctgatcttc gatgtgaacc gctgcaacac cggcgcgttc aaagttgcga
     5521 atggtacggg caatattgaa tgcaccgccc caacccacat cgatgtccac cagcagaggc
     5581 gtatccacgt tgtccgtaat gcgtcgtgcg tcgatcagca catcttccat tgtggtaatg
     5641 cccaaatcag ggataccgca agaacaggct gccacgccgc cgccggacag atagatggct
     5701 ttgaaaccgc tttgggtggc caatcgtgca aaataagcat tgacgcaacc ggcgacggca
     5761 agcggattcg attctttcac ggcttggcgg aaacgtgctc cggcagagtg ttgactcatc
     5821 atatttctcc tttatagact ttttttcagt atggacaggc ttccatcaca ttcggacggc
     5881 aaaacacagc catccgacgc gtcgggcatc ccaatccaat cattaaaaat atatgggaaa
     5941 aattatctta ttgatattta aaacgaatca aagaaaaaca gcagaccgtt cggaattatg
     6001 cggcaaaacc gcagacaaga agaaaacaag gggattattc agaaaagggg aaaacatctg
     6061 aattggtttc atagtaatgt tcctttgtag tgttatgtag ttttattttt cgataatcta
     6121 aaacacaaga ctgaaaatgt caaacatttt taaggagact cattacataa ttttaatatt
     6181 ttattttaat tccatgatta aactagatta attttgaatt atttgtttca ataaaattgt
     6241 caggaaatca agaaaaattt tcgataatca aaatcctgaa aacttataaa atattttata
     6301 cataaaaaca atgtattatt tttaagcaat ccgatgtatt ttattatata tttgattttt
     6361 atataattta ataaaaatta aatatatcgg caaacaaaat gccgtctgaa aacctttcag
     6421 acggcatttt taatatcgaa tcaatcagcc caaataccag aatttcagtg cccacaatac
     6481 ggcaacaatc cataccatag gcggaacgtc tttggtgcgg cggcataaaa gtttaaccac
     6541 ggcatagctg atgaagccga aggcgatgcc gtctgcaatc gaataggtaa acggcatgaa
     6601 gacaatggtc aggaatgcgg gtgcggcttc cgtcatatcg tcccaatcaa tatccctcgc
     6661 actgcggagc atctgcgtgc cgacataaag cagggcgggc gcggtggcaa aagcgggaac
     6721 acttttcgcc aaaggtgaaa acatcaggca ggcgagcatc aatacgccga cggtaaccgc
     6781 cgtcaggccg gtccgtccgc ctgccgatac gcccgccgcg ctttccacat aaggcgtggt
     6841 ggaagaagta cccaaagccg cacctgccac aatggcggta gagtctgcaa gcagtgcgcg
     6901 tttcaggcgg ggcagcttac cgtccaccag cagcccggca cggtgggata tgccgaccag
     6961 cgttccggta ctgtcaaata gatcgaccaa gaagaagacg aaaatcacac tgaccatgct
     7021 gacggtaaac aggccttcaa aatccatctg cataaaagtc ggcgcaatgc tcggtacttc
     7081 gccgatgatg ccgtgaaatt cattcaaacc catcaggctg gcaatgacgg taatggtcaa
     7141 gatggtgatg atgattgcgc cttgaacgcg gaaatgtccc aatacgacca ccatagcaaa
     7201 accgaacaat gccaacaacg cggacggctg atgaatatcg cccaaaccga ccaaggttgc
     7261 cggattggca acgataatgc ctgcgccttt cagggaaatc agtgccaaaa acaaaccgat
     7321 accggcagca atcgacattt tcaaacccat aggcagtgcg ttgaccagca tttccctgac
     7381 tttaaaaaag ctgaacagga taaaaatcag accggagatg aacaccgcac ccaacgcaac
     7441 ctgccaaggc acgcccatac ccttaacgac ggcaaaggtg aaataggcat tcagccccat
     7501 ccccggtgcg agtgcaatcg gatagttgcc gacaaaaccc ataacaaaac agccgatggc
     7561 agacgcgata caggtagcga cgaataccgc ccccatatcc atgccggtct cgcccaaaat
     7621 cagagggttg acgataacga tgtagcacat cgtcaaaaaa gttgtcaaac ccgccatcaa
     7681 ctcggtacgc accgtcgtac cgtttgcctt cagcttaaaa atcccgtcca acagtgtttg
     7741 ttttgaagtg tccatatctg aaagtctgta tcaagtggaa aaatctgaat ccgccgaccg
     7801 caaaccgtac gaaattgcaa catcttgaaa aacatacctc ccaaaccgga caaaccgatt
     7861 cgggaggcag caggtttacg ggcaaacagc acattatttg tctgaattgt gttgacgacg
     7921 caaaatcgca gggatttcaa agtcgtcaag tacggactga ttgtcgaaat ccgcagcggt
     7981 aaggttcatc gtgcggatac cgcgattggt gcggatcata ccttcgacat tgtggctttg
     8041 ctcctgtttg gacggagcaa ccgcttctac ctcccttgcc ggaacaaaat cgaccgcgcc
     8101 tttttctttc agaccggtag cgataatggt aatccggatg gcatcttcgc tcatggtctc
     8161 gtcttcagcc gcaccgaatt tgcattccaa atcgggatgc gcgctttggt tgacgatttt
     8221 catgacttcg gacaactcgg acattttcaa gcaacccgga gcagtcgtaa tattgaccag
     8281 cacaccgcgc gctccgtcca aggttacatc gtccagcagc ggactggaaa tggcctggtc
     8341 ggtcgccata cgcgcacggt cgataccttg ggcataaccc gaacccatca tagcgatacc
     8401 gcggttgctc atcacggttt tcacgtcggc aaagtcgagg ttgatgattt cgctcgggca
     8461 agttaccact tcggaaatgc ctgcgaccgc atcgcgcaat acattgtcgg cggcacggaa
     8521 ggcttcgcgc atcgttacgt cttcacccaa tgcagtcatc agtttgtcgt tcgggatgat
     8581 gatcagcgaa tcgacgtgtt ctttcaactg ttccaaccct gcctgtgcga catggacgcg
     8641 cttaccttca tatgcgaacg gtcgggtaac cacggcaacg gtcagaatgc ccaaagactt
     8701 ggcaatctca gcaacaaccg gcgcggaacc ggtaccggta ccgccgccca taccggtcgt
     8761 gataaacagc atattcgcac cgcgaatggc ttcttcaatg gcttcccggt cttcctgggc
     8821 tgccgcacgg ccgatatcgg gattcgcgcc cgcgcccaaa ccgcgtgtca gattcgtacc
     8881 caactggatt ctcttcgccg catggttttt tgccagagac tgcgcatccg tattggcact
     8941 gataaactcc acaccgcgca cattgttggc aaccatgtta ttgattgcat tgcaaccgcc
     9001 gccgcccaag ccgattactt taatcaccgc agggctgact gccgattctg ccacgtcgta
     9061 aacaaattcc attcaaaaac tcctgctcgc cccattcaga ggacggttta aataaattat
     9121 tattcattat ataagaagta tcttgctgct ggcaaaatac ttctcacctg tcaaacggca
     9181 atccacctgt tcagaagctg ttttcaatcc accgtttcaa tcttgccaac aaaccgccgc
     9241 ccccttccct ctcttgcact gcaccgtttt ccggctgcgg caagtttcct tccagcttgc
     9301 atgctgcatg aagcagcccg atggcggtag aaaagcgcgg tgtgcggacg cggtcggaca
     9361 aaccgcccat ttcttggggc gcaccggtgc gtacaggcaa atcgaagatt ttttcggcaa
     9421 attccacaat cccggtcatc atggacacac cgccggtcag aacgataccc gcattcagca
     9481 cttctttggg gaaacccgat ttttgcagct cgcccagcac tacgccaaaa atctcctgaa
     9541 tacgtgcgct gatgattgct gccaaaacct tactggaaac ctgacgcgat gtccggtcac
     9601 ccacgcccgg aacttcaatc atctcaccca agccttccgt atcgcatgat gccacgccat
     9661 aatggatttt aatgtactcg gcggcatcga gaggtgttct caacgatttg gacaaatctt
     9721 tggtaatcag attaccaccg gccggaatga cggacgtatg gcggatggca ccgttcatat
     9781 aaacggcaat atcggtcgtt ccgccaccaa tgtcgatgac gcatacgccg aggtcttttt
     9841 catcttcagt cagcaccgcc tgcccgcttg ccaacggctg aagcatgatc tgatcgcttt
     9901 tcaaaccgca ccgctcgata catttttgga cattctgcac tgccgtactt gcaccggtaa
     9961 tgatgtgcac ccgcgtatcc agacgcacac cgctcatacc gatgggctcc ctcacgccaa
    10021 gttgggtgtc aataatgtag tcttgaacca cggcatcgag aattttttga tcgggcggga
    10081 tattgattgc ctttgccgtt tcaatggcgc gatcgatgtc tgcctgcgtg acttccccat
    10141 ctttaatttt aaccacacct tgcgaattga gactgcggat gtggttgcct gcgatacctg
    10201 tggtaacgtg agtaattttg gtatccgcca tcagctcggc atcattgacc gcctgcctga
    10261 tggcttggac ggtggcatcg atattggtta ccatgcccgc gcgcaagccc cgtgaaggag
    10321 cctgccccaa accgacgatg ttgattttgt cgtcatcttg aacttccccg atcagtgcga
    10381 ggactttaga cgtaccgata tccagtacgc tgatgtatct ttgctgctgt tccattgttc
    10441 gtctgctctt aaaactgatt gaaatttgcg tcgcaccgtt tcagacggca cggccgtaat
    10501 ctgtccgata cctgttccca ctattcttcg gattcttttt cgggtaaacc gtcggaagca
    10561 tagcggactg aaaatccgtc cttatacctc atatccacat aggataaccg atttttattt
    10621 ttacgcaaca gatgctgcca cgcttcggta aaaagccgga ggcgtttcat ctcgttttcc
    10681 cttccgagcc tgacggtgat gccgttgtcc aaaacgacaa tccacgccga acgtgccgta
    10741 taggtcatct ctttgatgcc caaaccctgt tttgccaaaa cagtcgaaaa ttcgtcataa
    10801 cggcggagca tttcggcaga cgttccttcc gcgcctctga ataccggcat tccgggtctg
    10861 tccaagcggg cttcaaaaac attgccttcg ccgtccacca aggcatggtc gccccaacgc
    10921 gcgaccggct tgcgctcggt caggacgacc tcaaccgtgt cgggaaaacg gcggcgcacc
    10981 atgaccgacg caatccacgg ataccggcgg taggcctcct gtgcgccatt gatgtccgtc
    11041 ctcaaaatat tcccatggat gtattctttc gccaaactgc ccaatgcttt cttatcggaa
    11101 taaaccaggt tgcccttcag cgacacctgc ttgacgggca gatgattcga attgtaaaac
    11161 caaaccagcc cggacgcagc aagcagcatc gccatcatga caagcagcca gcgcgtcagc
    11221 cgttccatcg cttcggcatt atcccacatg tgcggtcttc aaaatttcaa tacataaatc
    11281 ggcaaaaccc acgcccgtaa cggcagcgga tttcggtact aaactatggc tcgtcatacc
    11341 gggcagggtg ttgatttcca acagatagag tttgccgtcg gtatctttga ggaaatcgac
    11401 gcgcacgcag ccttccgcac cgattgcctg cgcgccgcga accgccagtt cgcgcatcag
    11461 gctttcttcg gcttcggtca gatcttccga aggacattga taaatggtgt cgtcgcggtt
    11521 gtacttggct tcgtagtcgt aaaactcggt cgcggggatg atgtgtatgc cgggcaggcc
    11581 tttgccgttc aggacggggc aggaatattc gccgccgccg ataaaccgtt cggcaatgat
    11641 ttcgccctga aggtgtttca attcttcgta aacgcttttc agacggcctt tttctttgac
    11701 ttttaccaca ccgacgctgc tgccttcggc cgccggtttc acaaacatcg gcaggcccaa
    11761 tttttcttcg acggcatcga aatcagtgtc gtcgtgcagg acggcgaact cgggaacggg
    11821 cagccccaat gcctgccaaa tcagtttgca gcggtatttg tccatgccga tggcggaggc
    11881 ggcgacaccg ctgccggtat agggaatgcc caacagttcc aacgcgcctt gaaccgcccc
    11941 gtcttcgccg taagtaccgt gaaggatgtt gaatgccgtc tgaaaacctt gtgccttcaa
    12001 ttcagacaat ggggtttcct tgggatcgaa ggcgtatgcg tctatgcctt tgctttttaa
    12061 agcgttcaaa atggcggtgc cgctgtccag cgagatttct cgttcgctgg aaaaaccgcc
    12121 catcaatacg gccactttgc caaaattctg cattgttttt tgttctttcc tgattgcttt
    12181 atgcttgttg ccagaggtcg tctgaaacct gatttgcggt ttcagacgac ctttatatga
    12241 tgttccgtct gtcaggaggg tgtgcctcaa atctgtttcg acaattccag tagcgcggcg
    12301 ggaacgcggt tgatgcttcc cgcgcccata ttcaacacga tgtcgccgtc ctgcaaaacg
    12361 ttcaacagca tttcgggcag atcggcaacg ttttcgcagt aaatcggctc gagtttgccc
    12421 aacacgcgga tggcgcgggc aagagcgcgg gaatcggcgg cggcaatcgg ctcttcaccg
    12481 gcggcataaa cttcggtcag caccagcgcg tcaacggtat tgaggacttt ggtaaagtct
    12541 tcaaacaaat cgcgcgtgcg ggtatagcgg tgcggctgga aggcgagtac caaacgtttt
    12601 tccggatacg cgccgcgtgc ggcggcaagg gtcgccgcca tttcgacggg gtggtgtccg
    12661 tagtcgtcca ccaagagcgc ggtcccgccg tttggcaact tgatgtcgcc gtatttttgg
    12721 aagcggcggc cgacgccttc aaagccgagc aagccttttt ggatcgcttc aaccgatgcg
    12781 ccgacttcca gcgccacgcc gatggctgcc aatgcgttca gcacgttgtg tctgccgggc
    12841 atattcagca cgacttcaaa cgacccctgc tcatgtcctt tcatttgaac atggacggtg
    12901 aatttcattt gcgcgccgac gttttcgatg tcggtggcgt agatgtcggc ggtatcgtcc
    12961 aaaccgtaag tagcataagg tttgctcact ttgggcaaaa tcgcgcggac gtgttcgctg
    13021 tcaatacaca aaaaggcttt gccgtagaag ggcatacggt ggatgaaatc gataaacgcc
    13081 tgatgcagct tctcaacact gtgcccgtag gtatccatat ggtcttcgtc gatattggta
    13141 acgacggaca taatcggtgt caggtgcaga aaggatgcat ccgactcgtc ggcttcggca
    13201 acgatgtatt cgcctttgcc caagcgggcg ttggtgcctg cggcgttgag tttgccgccg
    13261 ataacgaaag tcgggtcaag tcctgccgcg ccgaggatgg aggcggtcag gctggtggtc
    13321 gtggttttgc cgtgcgtgcc ggcaatggcg atgccgtcac ggaagcgcat caactccgcc
    13381 aacatcaggg cgcgcggaat aacgggaatt tgctgctcca acgcagcgac aacttcggga
    13441 ttttcttttt tgacggcggt agaggtaacg acgacatccg caccgttaac gtgttctgcg
    13501 gtatggccgg gataaacttg aatgcccagg ctgcccaaat gctcggtagc ggcatttcgc
    13561 gcctgatccg aaccggaaac tttaaaaccc aaattgtgca agacttcggc gatgccgctc
    13621 atgccgacgc cgccgatacc gacaaaatgg atgttggtaa ctcgattttt catcataatg
    13681 ttgcgttccg gttggttttt agtgcgtaaa ggcgttattt taaagggctg accgtttgcg
    13741 cgccatagtt ttctgacaaa tatatagcgg attgaaataa aaaacatcca tgccgtctga
    13801 acggcttttc agacggcatg gttcggcagt ttacgccgca cacgcaatcg cggcttcggc
    13861 aacgtcgtcc gcactgtgcg gcagtgccaa agtacgggcg ttttctgccc atttgagaca
    13921 tttttcgcgg ttcaggctgc cgaggatttc ggcgagtttt tccgccgtca actgggtttg
    13981 cggcaacagc aatcccgcct ccgcctgcac cataaaacgt gcgttggcgg tttgatggtc
    14041 atcaacggcg tgaggatacg gcactaacaa cgcacccaat cccgccgccg tcaactcggc
    14101 aatcgtcagc gcgccggcac ggcaaatcac caaatcggca tcgcggtagg cggacaccat
    14161 gtcggtaata aattccacac attcggcttt cacgcccagc gcgtcgtaat ccgcctgcaa
    14221 gctgcccagc ttgccccgtc ccgattggtg gtacatctgc ggacgcgcat tgtcgggcag
    14281 caaagccaat gcctgcggta cggttttgtt caaaacgtcc gcgcccaaac tgccgccgac
    14341 caccaaaatt ttcagacggc cttcacgccc ttggaagcgt tcggcaggca cgggcaggtt
    14401 gctaatatcg gcgcggacgg ggttgccgac caagccgcct tcgtggctga acgctttcgg
    14461 aaaagcgtac aacacccgct tcgcccagcg cgacaggtgg cggttggaca aacctgccac
    14521 ggcgttttgc tcgtgaatca caatcggcac gcctaatagc ttcgccgcca aaccgccggg
    14581 gaaggtaacg aagccgccga agccgatgac gcactcgaca cggtgtttgc ggataatccg
    14641 ctgcgcttcg cggacggttt gatacaaagt aaacggcagc atcagcttgc gtttgatgcc
    14701 gttgccgcgc acgcctttaa tcgccagcgt ttccaagcgt atgccgtatt gcggcacgat
    14761 acgctcttcc atcgaatcct tgctgcccag ccaaatcaca tgatggccgc gcgcgcgcaa
    14821 tgaatccgcc accgccagcg cggggaaaat atgtccgccc gttccgcccg ccatcagcat
    14881 aaaggtttta ccgcccatga tttactccac ccgataaccg cgcattttcc ggcggttttc
    14941 ataatctata cgcaacagca gcatcatgct gatcagcatg aaaaagactg acgaaccgcc
    15001 ataggacatc aacggcagcg tcagaccttt ggtcggcaaa gcaccgatgt tcacaccgat
    15061 attgaagaaa ctttggatac cgatccaaat gccgataccc gaagcgatat aggcgttgaa
    15121 agtcaaaccc aaatcgcgcg actgcttgcc gatggaaaac gcccgcacca ccagccagcc
    15181 gtaacagaat atcagcacac acataccgaa gaaaccgaat tcttcggcga tgatggcaaa
    15241 aataaaatcg gtatgcgctt ccggcagaaa gccgcgtttg ctcaaactcg cacccaaacc
    15301 cataccgaac cactctccgc gcccgattgc catcagagag tgggtaagct ggtagccggc
    15361 accctgaggg tctttccacg ggtccaaaaa tgccactacc cgctgcacac ggtagggagc
    15421 ggcggtaatc atcagcacca tcccgcccaa gacgctgcct accaggacga aaaaatattt
    15481 ccacggcaat cctgccaaaa acagcattcc aacggcaatg acggtaatga cgacaaacga
    15541 accgaaatcc ggctgtacca ttatcagcac caaaccgaac gccaccagca taatcggcag
    15601 gatgatcgcc cggaaacggc cgtacatttc taatgtttca cgacgtgcct gcggattggt
    15661 ggcggacatg atcagattgg ccgtcccccg ccaaatcgac tgccaaccca aactttccat
    15721 gctgcgcaac acttcttcac ggcgcgtgaa caggcttgcc aaataaagga tgaccgccag
    15781 cttgaacagc tcggtcggct ggaaattcaa cggacccaaa ggtatccaac gggtcgcgcc
    15841 attgatttcg cgcccggcaa tcaatacgac taccagcaac aggccggata gggcaaaaat
    15901 ccacggcaca agccgccgcc atgtcctcat cctgcaaaga aaccataaaa aaccgctcgc
    15961 tatcaagccg gcaacgacga accccgcctg tctggtcaaa tagaaaaact gatcgccgcc
    16021 ttccttcgat gccaaataca cagaagccga ataaatcatc agcaggctga acgccgtcat
    16081 cagcaccacc atccacaaaa gcggcgcgtc gaatttcctg ccgtcgcgca caatcggtct
    16141 gtcgagcagc agagtgtgga caccgtcgcc cacttttacc aatacttccg aaatcttcaa
    16201 aaaaaccacc tgccagtctg tttgcacctg ccgcaaaggg caaaaatttc agacggcaga
    16261 caatgccgtc cgaacatacg atacatccca aatcggtatt ctaaatcttt acttgccgcc
    16321 caacaatgac ggcgtttgca tttcagacgg catcacaaag ccttaaacgc ttcgataaac
    16381 acttccgaac ggtgcgcgta gcctttaaac atatcaaaac tcgcacaagc cgggctgagc
    16441 agcacgatat cgcacgcttc ggcttgggca tatgccgtct gaacggcttc ttccaaagtg
    16501 gcgcagtcgg tcatattcag accgcagccg tccaaatcgc ggcggatttg cggcgcatca
    16561 acgccgatca ggaacacgcc ttttgccttg ccggcaagcg cgtcgcgcag aggcgtgaag
    16621 tcctgccctt tgcccatgcc gcctaaaatc acgaagagcg gattttgcaa accggcaatc
    16681 gcggcggcgg tcgcgccgac attcgtgcct ttgctgtcat caatgaatac cacgccgttt
    16741 ttcttgccga ttttttccac gcggtgcggc aggccttgga aggttttgac gtgttcaagc
    16801 aatgcttcgc gcggcaaacc gacggcttcg cacaaagcca cggcagccat gacgttggcg
    16861 gcgttgtgca agccttgcaa cggaatgtct ttcgtggcaa tcaaatcttc attgccttgt
    16921 ttcaggcggc ctgtctcacg ttccaaccaa aaatcggctt cgtgttccaa cgaaaaccat
    16981 tttacctcgc gcccggcacg tttcatggca cggcagaacg catcgtccgc attcaaaacc
    17041 tgcacaccgt cgccacggaa aatcttggct ttggtatgcg cgtagtcgag caagtcgtcg
    17101 tagcggtcga gatggtcttc ggaaatattc agtaccgtcg ccgccgtcgg gctcaggctt
    17161 tcggtgtttt ccagttggaa gctggaaagc tccaacaccc acacgtccgc ctttttgcct
    17221 tcgcgctgcc attccgcctc caaaaccggc gtgccgatat tgcccgcgat aacggtatcc
    17281 agcccgcact tgatacagag atagccgacc aggctcgtta ccgtggtttt gccgttgctg
    17341 ccggtaatcg caattacctt gtcgccccgg cggttcacaa tgtccgccag caattcgatg
    17401 tcgcccaaca cgcgtccgcc gttttgcttg aacgcctcaa tatccggctg acgctcgctg
    17461 atgccggggc tgagtgccaa aatatcgaaa ccgttgtcca gcgcatcttt cagacggcct
    17521 gtgtaaaaca ccagcccgtc aaacatctta ccgatttgcg acacgcgctc gggcttcaac
    17581 tccgcatcat aagcggccac ctccgcgccg tttttgcgca gataggcaat catggaaata
    17641 cccgtaccgc cgagtccggc gacgaggatt tttttgtttt caagcgtcat tttggttttg
    17701 tcctaaaaca aatcatattg ggcaggtggg gggatgtccg cccctgccca agccgctttc
    17761 agacggcatc gcgggctgtt caataacctg ccttcaggcg ttggtaaatg tcgcagccgt
    17821 cttggtctcc gttttgacaa gccttaccaa accattcttg agcgagcaca aggtcttggc
    17881 gcacgccgcg tcctttgtaa tacattgcgc ccaaattgta ttgggctgct gcatttccct
    17941 gtgctgccgc ctgcaagttt tcccgaaaat ccgatacgtc atccgcccac accgcttggt
    18001 tcaagcccaa ggcaatcagg gcggcggcaa gccatttgac tgtctgtttc atggttttac
    18061 ttctgtttta gtataagccg ccgttaacga tagggctggg cggattgtcg ccgcaggttt
    18121 attgcgcgtt caaatgccgt ctgaaaggtg ttcagacggc ataggttcag cggattttga
    18181 gggtactcaa accgatcaac accaagacga tggtaataat ccaaaagcgg acgacgactt
    18241 gggtttcttt ccagcctttt tgttcgtagt ggtgatggat gggcgccatc aggaagatgc
    18301 gttttttggt tttcttatac cagccaacct gaagcataac ggatacggct tctacgacaa
    18361 ataatccgcc cataatgacg aggacaaact cttggcggac gatgacggcg acggtaccga
    18421 gcgcggcacc caatgccaat gcaccgacat cgcccataaa gacttgcgcg ggataggcgt
    18481 taaaccacaa gaaaccgagg cacgcgccgc acatggcggt acagaaaatc accacttcgt
    18541 ttgcgccggc aacgtaaggt aattgcaggt attgggcaaa ttgtgagtgg ccgctggcat
    18601 aggcgaagat ggcgaggccg gcggcaacga ggacgacggg gaaggtcgca aggccgtcca
    18661 agccgtcggt gaggttgacg gcattggatg tgccgacgat ggtcaggtaa gacaacacca
    18721 aaaagccgac cacgcccagc ggcagggcga tttgtttgaa gaacgggaca atcaaaatat
    18781 tgttggcgga attggcggca aggtaaaaca atgccaaact ggcgataatg gcaacgcttg
    18841 actgccacac cattttgaat ttggcggaca cgccgttggg gtctttatag acgactttgc
    18901 gccagtcgtc gtaaaaaccg agtgcgcccg tggcgagcaa tacgcccaag agaatccaga
    18961 tatacgggtt tgcccagttg ccccacaaca gggtggacac ggtaatggcg gtcagaatca
    19021 gcgaaccgcc catcgtcggc gtgccgtttt tgacgaggtg ggtttgcgga ccgtcggtac
    19081 gcactgcctg cccgcatttg agcgcggtca gcctgcgtat cgtccacggg ccgaacatca
    19141 gggaaaacgc taaggcggtc aacgccgcca tgacggcgcg gaatgtggtg tattgaaaaa
    19201 tattcagacc ggttaaccag ttgctgaaat gtgcgagcca taaaaacatg gggcttcctt
    19261 tttttgtttt gtcgttatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc
    19321 tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc gtactatttg
    19381 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata catcgggcct
    19441 gcgcccgttt gaggttggct tgccgcccgg caaggtttcg gacggctttt gcagattaac
    19501 gtgcagggac tttctcaatc acgcaaattg ggtaactccc cggattttta ccgccgccca
    19561 agtctaaaca taaatcttca taaagatacg cctgcgtccg cattccggca taaaacgcgc
    19621 cgaccagcgc ggcaatcagc aataaggctt taaaaaaacg tcggcttttc attttcactt
    19681 atcctccaat gcctcgacca cttcttccat ctgcataaag cgcgaacctt tcaccaacac
    19741 ggtggcgcgt tcgggcaaat cgtggcgcaa cacttgaatc aacgggtctt tggcggcgaa
    19801 ccacaaaccg tccgcgccaa atttttccgc cgcttcgacg ctgttgtcgc cgacaaaata
    19861 agccgcttcg atgccttggt cgcgggcata cgcgccgact tcggcgtgca tagcggcggc
    19921 ttcgtcctcg cccagttcgc ccatatcgcc cattacgaaa atacgcggcg caggcatacg
    19981 cgccaacacg tcaatcgcgg ctttcatgct gtccgggttc gcattataag tatcgtcaat
    20041 cagggttgcg cccttgattc cggatttgac gttcagacgg cctttgatat tgctgaagcc
    20101 tttcaaacct tccgccacat cgttcaaact caaacctgca gccaaagaca gcgcggcggc
    20161 agcggcggcg ttgtggacat tgtggcggcc gggaacaggc agcaccacgg cggcgcgttc
    20221 gtcgccgcac accaaatcaa attcgcacga caacggtttc agcacgatat tttccgcgtg
    20281 gacatcgccg ctatcgatgc cgaaagtgcg cgtattcaaa ttaagcgttg ccgttttgaa
    20341 gacagccata ttggcatctt cttgaggaat cagtgccatg ccgtctgaac ataagccttg
    20401 ataaatctcg cttttcgctt tggcaatatc gcccactccg tcgaaaccgc agccgacatg
    20461 ggcgcgcatg gcgttgttga ccaatgcggc atcgggtttg gcgatttgtg tcaaaaccgc
    20521 cagttcgcca aaatggttca tacccatttc aatcacggca tagcggtgtt tttcgtttaa
    20581 tttcaacaaa gtcaacggca atccgatgtg gttgttgaag ttgcctgccg tcgccaaaac
    20641 ggcatcatca ccgaaacggc ggcgcaatac cgcagccagc atttccttca ccgtcgtctt
    20701 gccgcccgaa ccggtaatgc cgaacacaaa cggatccacg ttttcacgcc acgccttcgc
    20761 catcatttgc aacgcggtaa gcgtgtcatc gactttcaac gcgccatcca ttgcaacgca
    20821 atcttcgcgc gaaaccacaa ccgccgccgc accagcagcc aatacgtctt caacaaaatc
    20881 atgcgcatca aaccgcccgc ccgccaatgc gaaaaacaca tcgcccgcgc ggatgtcgcg
    20941 gctgtcggtt acgatgcgcg acacgggttt gctttcagac ggcatcggaa gcttgagggc
    21001 ttggcagatg aaatttaggt ccagtggttt catatttact ttcgttaata ttcgggcggc
    21061 ggacacatcg gtagcggctg atttttttat cgcctgtttt gctgtggtaa aacacagatt
    21121 attttcccat tctcattcgg cattttttct gtacgtatca ttttttagac gtatttttag
    21181 tcgatttgcc ttttcccgca taccacggcg cggggtcgtc gggcagtccg tcgataaagg
    21241 caaggttatt gccttcgccc tgcacatcgg gaacattccc ccaaaaatca tagccgtcat
    21301 cgggcaactc gtcggtttcg atacccgtcc aactgccgaa tccgcgtaaa aaattaaacg
    21361 cgctgcgcct cactttaatc atttcgcgcc gcgtgtcatc ggacgaaatt ttaaccagcg
    21421 gcacgccgta gctttgacgc ttccacgcac catgtgtcaa cgttcgctcg ccgcctttac
    21481 cgacatgcat caaaccgtgg tcggaaaagt aaaccaaaga ccagctttct ttattttcat
    21541 tcaatatctt aactgtatct tctaaaaatt tatcggtttg cgcgatggtg gaaacatagc
    21601 aggatatttt ttcagtttga tactgaaacc gccgcgcatc cttatccaaa cgtgtgcaaa
    21661 aatcactgtg cgaacccatc aggtgcatca caatcagccg aggcttcgtg cctgcatttc
    21721 ctatcaaaac ccgtttgaac gccggcaaca aaaggcggtc gctcaacccc gcgcttttgc
    21781 cataatcacc cctttgggta aaccacggat aatcgctgcg tagggcatag gtggaaattt
    21841 cgttggcaaa atgccccaac attccttgat tagacagcca cgccgtccga aaacccgcct
    21901 gcttcgccaa gctgacgatg ttattgttcg gttctcccgg cagccccaaa gtctgcggca
    21961 gcgaaagatt cgtcgcgtgg gcggtcgatt ggtaaccgtt tatcaacagc cctttggtct
    22021 gactcaaaaa aggcgtatcg ggcaatggga aaccgtaaac attcatataa tccgaacgcg
    22081 cgctctcacc gataaccaca acataatttt tatacttggg cgcaacatga cggatatgcc
    22141 atgttgacgc tttttttgct gcttccaaaa tgtgggcgcg cttggcgaca tattgtgccg
    22201 gcgcggaagc caaatcgtaa tacaggtcga aaatattcaa caacaggccg gcatcaggtt
    22261 ctcgcaaatc tttatcgctg gcgattttat ccatcaccgc gcaggacaaa atcaaagtca
    22321 ataatatagt cagccatatt ttgctgcggc gtttatagtt ttttacgtca gcaaataccc
    22381 cccccgatac acaatatttc caaactgtca agccaaaaat gaaaaataat gcctgcacaa
    22441 aataaagcga cccgggaaga ttgccgacaa attcacgcgc ctcggcagga ttgctttcca
    22501 atatcgaacc gactatctga taagacggcg caccatacaa ccagccgacc ggcaaatata
    22561 gggcggttgt gccgacgtaa atcagtaaaa caactgaaga aacgcgcggg aaaccgcgtg
    22621 ccaataataa taaaaataca accgaagcca aagcacccac ggcagaataa cggtagccgt
    22681 attcatattc caagtgataa cctgatgcga tggccgcgcc aaaacaaaag gctaaaagcg
    22741 cgcaaatgcc caatgtattt tttaatgtct gatccatatt ttttattcgg gcggattgtt
    22801 tcatacgatg ccgtccgaat atcccattat ttacgagtta acaaagcctg tccgacgatt
    22861 tcaagatcgg aaaaacggtg cttcacgcct tgtacatcct gatagttttc atgccctttg
    22921 ccggcaatca ggatgatgtc gtttgcggcg gcttgttcaa ccgcataacg gatggcggcg
    22981 gcacggtcgg cttcgacgca ttcgggcgcg ggaacggcag gcaggatgtc gttgatgatg
    23041 tcgtgcggat tttccaaacg cggattgtcg ctggtgacga cgactttatc cgcgccctgt
    23101 acggctgccg cgcccatcag cgggcgtttg ccgcgatcgc ggttgccgcc gcaaccgaat
    23161 acgcaccaca aagccgcacc ctgcggtttg atttcctgca aggtggcgag tgctttttcc
    23221 aatgcgtcgg gcgtatgggc ataatcgaca acgaccaaag gtttgccgct gttcatgatg
    23281 cagtccatgc gccctgaagc gggacggatt tttgccagca catccaatac attatcaagc
    23341 ggatagccgt tggcgcacag caaggcgatg caggcggcga ggttttgcgc gttgaaccgt
    23401 ccgagcaggc gcgtgcggca tttcccttcg ccccacgggg tttggaatac agctgctatg
    23461 ccgtctgaag aggcggtgaa atcgataatg cggatgtcgg cgtgttcgct gaagccgtag
    23521 ctataaacgg ccaaatcggg acagtctttt ttcagacgac ctacgagttc cgcgccgtat
    23581 tcgtcatcca cgttgatgac tgcgtgtttc aagccgtgcc agtaaaacag gcgcgacttg
    23641 atggcaccgt aggcttccat cgtgccgtgg tagtcgaggt ggtcgcgggt gaggttggta
    23701 aagattgcgc tgcggaatga cacgccgttg acgcgcgact ggtcaagccc gtggctggag
    23761 acttccatcg cggcgactgt tgcgccttgt tgacggaaac ggtagagcag ggtttggaca
    23821 tcgacggggg cgggtgtggt atgcgtggtt tcttccaatg caccccaaaa gccgttgccg
    23881 accgtgccga caatggcggt tttttcgccc aacaaatcgg cagcttgcgc cagccattgc
    23941 gtgatggagg ttttgccgtt ggttccggtt acgccccaga atttgagacc gtctgaaacg
    24001 ttgccgtaaa cttgcgccgc caatatgccg gcacggtgtt tcaaatcttt gatgccttga
    24061 ttggggactt tccattcggg attccacgca aatttgccgt cgtcgtccca aaaaacaaaa
    24121 gccgcgccgt tggcaacggc ggcggggata taactgcgtc cgtcggcata ttcgcccgga
    24181 caggcaacga aaatatcgcc ttgtttgatt tggcggctgt ctgaatgcaa caaacgccct
    24241 gccgcgtttg cacacgacag ggtcgggatg ccggtttcag ccaaaggggt taacttgctg
    24301 aacataaaac aatctcgttg atactcggat taagacggtg ttttgacggc tgcggcggtc
    24361 aggggcttgg tcggggaaat gcccaagatg ttcaggctgc cgcccataat ttttttgaag
    24421 ggcggccctg ccactacgcc gccgtaataa ccgtgggcag tcggttcgtc aatggttacc
    24481 gccacaatca cacgggggtt tttggcgggg gcaaaaccga taaaggtagc gatgtgtttg
    24541 ttgtcggcat aacgcccgtt gacgaacttg cgcgccgtgc cggttttcgc gccgacatcg
    24601 aaaccgtcca ccgcacccgc cgtaccggtg ccgcccggct cggtcacgga aaccatcaga
    24661 ttgcgtacct cgcgcgcggt cgattctttg aatatgcgtt tgccttgcgg cgcaaccgcc
    24721 tgtttttcaa agctgaccgg cagcaaaacg ccgtcgtgcg tcagtgcggt ataggcgcgc
    24781 gccaattgca gcaggctcaa ttgcaggccg taaccgaaag acatcgtcgc ctgttcaata
    24841 ggccgccacc tgcgccaatt tctcaacaaa cctgcagttt cgcccggaaa gcccgagtgc
    24901 atacgcacac cgatgcccaa ctcatgatag aagtcataca tttcttcggc accgaaacgc
    24961 gcagacagtt tgcttgtgcc gacgttggac gatttctgca tgatgccgcg cacatccaaa
    25021 gaggggtaaa catgggtatc gcgcacggga gacggtccga ttttataagg ctgcgtattc
    25081 agccgttcgt tcaaatcggt tttgcccgca tccaatgctt tcgcaatcac aaacggtttg
    25141 attgccgaac cgggttcgat catatcggtt acggcacggt tgcgccgctg ttcgctgtct
    25201 gcccggccgg gcctgttggg atcgtaggcg ggcgtattgg ccaaggcgag gatttccccc
    25261 gtgcgggcat ccaaaaccac caccgttccg gcttttgcct gatggtattc gaccgccttg
    25321 ttcaactctt cataggccaa ggtctgaatc ctctgatcga gggaaaggat gatgtctttg
    25381 ccgtttttcg gggctttatt gcgcggggag tccaagctgt ccacaatatt gccctgccgg
    25441 tcccgcaaaa cgacttccgc gccgtcttcg ccatgcaggc tgtcttcaag cgaaagttcc
    25501 aaaccttcct gacctttgcc gtcaatatcg gtaaatccga tgacgtgtgc aaacaggttg
    25561 cccatcgggt aatggcgttt taattctttt tcaaatacaa agttttccaa acccaaggct
    25621 ttgacctctt cggcaacctt gggatcgagc tgccgcttaa tccagataaa cgacttgcct
    25681 ttctgttcga gcttgttcct caaaacatca accggcacat cgacaagctc ggacaggcgt
    25741 tccaattgtg cggcagacgg catttccttc atctctttag gcacggcaaa cagggactcc
    25801 gtcggcgcac tcaacgccaa aaccgcaccg ttccggtccg aaaccgtacc gcgtgtagcc
    25861 ggcaatgttt gagtccgcac aatccggttg tcgccctgtt ctttcaaaaa gttatacgtt
    25921 accgtctgca gatacagtcc gcgagcaatc agaccggcaa acaagaccgc tattgccatc
    25981 aggacgaagc tgatccgtcc gttactggtc atcggctttt tgacctgctc ttctttgggc
    26041 agcatccgag gcttatattc gctcttaatc aacattttta cttctcgtta ttattatcct
    26101 gacgcaggaa tccgattccg gcacacaggc tgcttctatc tttgatgcgc caccataaag
    26161 gtattgcccg aaaccggcgg atggaggttt tgtttttctg ccgccgccct gatcgcttcg
    26221 tggttcgcca aacgcgcctg ttgcagcctc atttgcgcat aatcctgctc caaggcgatt
    26281 tcctgttttt tcgccttgtc caaagctgtg aagttgagcc tgtactggtt ttgctgcatc
    26341 acaacggaaa aagcggaaac gcacaccgca agcagcagaa ggaaattcaa tttgttcatt
    26401 gccattcaga cgtttttctc tgtgattgtt ccggtatcga accggcagtc cgctccgcca
    26461 cacgcaaaac cgcacttctc gccctcggat tggcggcaat ttccgcctca cccggcttta
    26521 atgccctgcc cacgattttc aggggcggct cgggcaaatc cgcttccctg accgccgccc
    26581 agcgcggcag gggcgcgtgt tgcgaatatt ttttgacaaa ctgcttcaca atgcggtctt
    26641 ccaacgaatg gaaagcaatg accgccaaac gtccgccctc tttcagacga cacatgacct
    26701 gcggcaatac tgcccctact tcttcaagct cgcggttaat aaagatgcgg accgcctgga
    26761 aggtgcgcgt cgcaggatcc tgcccccgct cgcgagtacg gacgttttgt gccacgatct
    26821 gcgccagctt gcgggttgta tcgattggac tttccgcccg ttgcgcaaca atggcgcgcg
    26881 caatctggcg gctaaaccgc tcttcaccat aattcttgat tacctcgtgc aaatcctgtt
    26941 ccgacgcaac cgctatccac tctgcggcag acataccgcg cgtcgtatcc atacgcatat
    27001 ccaaaggggc atcgaaacgg aagctgaaac cgcggctgcc gtcatcgatt tgcggggacg
    27061 aaatccccaa atcaaacagc gcaccgtcca ccttgccgat acccaaaccg tccaatgccg
    27121 tctgaaacga agcaaaaccg ccatgcacga caccgacccg tttgtccgaa cgcgccagct
    27181 cttctgccac agcaatcgcc tgagggtctt tgtcgaaaac aatcaaccgc cccgcatcgc
    27241 ccaaacgcga caaaatcagc cgggaatgcc ctcccctgcc gaacgtaccg tccacataga
    27301 caccgtcttc gcgcacggca agcgcatcca ccgcctcatt cagcaagacc gtgatatgcc
    27361 ggtaactttc tgctccactc acaattgcaa atccgtctga cccaactgga aggcaagttc
    27421 gtcaggatcg tcatccaaag cctgaaccat ctcagcctcc cactgctcgc gaccccaaag
    27481 ctccaaacgg ttggcacgac cgaccaaaac gacttcacgg tcgaaatcca cccttttcct
    27541 cagtccggca ggaagcagca cccggccggc gctgtcccat tccaaaattt ccgcgttatg
    27601 cagcaaaaga ttttgaaacc gccgcaaaac agggttatcc gccactttta agtttaaaag
    27661 ttgcgccgca accttttccc actccgcaac agggtacatc aacagcttgt gtttcgactc
    27721 gagcgttacc actacggcag gcgtatagag gcgcgacaga atgtcacgga atttggcagg
    27781 aacagccaac cgccccttac tgtcgatgct taattcgtgt gcgccgccga acatgacatg
    27841 tcccaagccg aaatcaaaat cacaagggta aaagagacac tttgccccac aattccccac
    27901 caatcgacac tataagaaat tttaaacact cggtcaaatc agggcatgaa aactcattaa
    27961 catatctgaa atttttattc cttttaaaaa caataagata aaaaatgacg acaacggcac
    28021 ggcgggtgcg gtacagaata atcgaaccaa taaacaacta tatatgatta atttaataat
    28081 ataaacacaa tatatagtat taagataaag ccatgacagc acccgtacca acgtgtaata
    28141 tgtcgggaaa tccaataaat ttacacaagc taacacttat catgcccctc cccctcccct
    28201 ctcccgaagc acggcaattc tcgctcaaac tgcaaaccct cattgccgaa aaaatcggca
    28261 aacacggcaa ctggattcca ttttcacgtt ttatggaatt ggttttatac gctccgcaat
    28321 acggctacta caccggcggc agccataaaa tcggcaatac cggggatttt attactgcac
    28381 cgaccctcac ctctctgttt gcacagacac tggcacgcca acttcaagaa cttctatccc
    28441 aaacggcggg caatatctat gaattcggcg cgggaaccgg acagcttgcc gccgatttgt
    28501 tgggcagcat ttcggacggc atcagccgtt actatattat tgaaatatcg ccggagctgg
    28561 cagcacgtca gaaaaacctg attcaagccc gcgcaccgga agcatctcaa aaagttgtcc
    28621 acttgaccgc acttcccgaa gcgtttgacg gcatcatcat cggcaacgaa gtactcgatg
    28681 ccatgcctgt cgaaatcgtc cgtaaaaatg aaggcggctc attcgagcat gtcggcgttt
    28741 gcctagataa tgaccgtttt acctattcgg cacgaccgct gcacgactta cagctatctg
    28801 ccttggcttc cctctatttt cctcaaacag attatcccta caccagcgaa ctacatccgc
    28861 aacaatatgc ctttatccgc acccttgcct caagacttga acgcgggggt atgatattca
    28921 tcgactacgg ttttgatgca gcgcagtatt accaccctca acgcaatcaa ggtactctga
    28981 tcggacacta ccgacatcac gttatccaca atccttttga cttcatcggg ttggccgacc
    29041 tgaccgcgca tgtcaacttt accgacattg cccaagccgg tacggatgcc ggattagatt
    29101 tgataggtta ccttccccaa tcccatttct tattgaacct gggcattacc gagctattgg
    29161 cacagacggg gaaaacggat tcggcagcct atatccgtga agctgctgcc gttcagaaac
    29221 tgattgacca gcatgaaatg ggcgaactgt ttaaagtcat cgcattcgga aaaaatatcg
    29281 gcgtcgactg ggcaggattc cgcttcggcg acatctgcca caaactctaa ccctcatgcc
    29341 gcctgaatcc gcttcagacg acataaactt tttaacattt aaaaacagtc aactaattca
    29401 aaattaaaaa atacggcttg tcaaaaaaac agaaaaacat ataatagcgt cttcacgaaa
    29461 cggcgaatta gctcagtcgg ttagagcaga ggaatcataa tccttgtgtc cggggttcga
    29521 gtccctgatt cgccaccaaa ttttcggggg tatagctcag ttggtagagc gcttgcatgg
    29581 catgcaagag gtcagcggtt cgattccgct tacctccacc agataaaaaa gcacagaccg
    29641 taaaaaggta tgtgcttttt tattgcctga ttgccagcaa ataaagaata aaccactgcc
    29701 ttcaaaacag gcaatcgact ttaaacctat cgccccgcct gtcctgattt tatagtgaat
    29761 taaatttaaa ccggtacagc gttggctcgc cttgccgtac tatctgcggc ttcgtcgcct
    29821 tgtcctgatt tttgttaatt cactatatca gcccgccaga caaacccgac ccgaataatg
    29881 tcttcaggtc gggtttatgg tttcattccc aacttatcca gcctgacagc cacaatataa
    29941 tgatggcaat accaaaaaca atgcggtaat aggcaaaagg aatataattt ttcttggaaa
    30001 caaacctcag caacgctttt accgctacca agcctgaaac aaaggcagca ataaagccta
    30061 tcagaatcaa accgacatca tgcagggtga aaaatcggta atgtttcagg acatcataag
    30121 ccgttgcggc aaccatcatc ggcacagcca agaaaaacga gaattctgtc gcagttttcc
    30181 gttcgatgcc ccaaagcatc ccgcccataa tcgtactgcc cgaacgggac gtacccggaa
    30241 ccagtgcaaa cacttgggca acgccgatca tcaaggcatc aatcggacgc aatgcatcaa
    30301 catcggcaat tttaggctct gctcggcttt ggcgtttctc cacccacaaa ataaaaaaac
    30361 cgcccaaaac cagcatgact gcaacactca aggggttaaa cagatactct ttgatttgtt
    30421 tgccgaacaa cagccccatc acggcggcag gtataaaagc aatggcaaga ttaaggacga
    30481 agcggttggc tttccggtct tttcccaagc cgtgcaacac attgctgaaa cgttgccggt
    30541 attcaaacac taccgccaaa accgcaccga gctggatggc aatttcaaaa accttgtgat
    30601 tgctgtgaaa accaatcaga ttgccgaaca caatcaaatg tccggtgctg gaaatcggta
    30661 aaaattcggt aaaaccttct accaagccca tcatcagggc tttcaggaca atcagaaaat
    30721 ccattgcttg cgcttctttc ggatacggga gttcggctat ttctgtacag caggggtctg
    30781 acgcttgcgt tcttccctga ctttggcaac caattcttta atcgtatgaa cgccgccgtc
    30841 aaagccgtta ttgaagataa cgcggtattt tccgccgaca ataacggtcg gcgtgctgtc
    30901 gatgcggtat tgttccgtca gtttctgcat ttttaatgcg gcggcggcag cttcggggga
    30961 atcataggcg cgcatcagtt ttttgccgtc aaagcctttt tgagacaaag cccattttcc
    31021 ggcaacagcc ctgttttcca agcggatttt ttgttcgtaa actgctttaa acacggcagg
    31081 gtttgcctga tatttcaaac ccgacaaatt gacggcagcc gccatcctgg ccagaccgag
    31141 catttcaggc tgccagacca cgtgctccgt cctcaaatag gcatcagacg gcaatgcctt
    31201 gcccagtttc aataacaaag gatcgaaatg atggcaatgt acgcagaaat agccgaaaaa
    31261 ttccaaaacc tcaattttac ccgactgttc ttgaggaatg ggtttatcca acacaagata
    31321 gtcttcccct tccgtcaggg catatgcctg cgcggacaac gctgccgaca gcagcagcgg
    31381 caacagatgt ttgagcttca taattattta ctttcgatag aacggatcag gctggcgact
    31441 tcatgttttt tcaactcgtc ctgcattttt ttcaccgcat cggcagacat attgccgctt
    31501 tgcacccggt aaagcgtttt atgtcccgcc tgataaccga ccaccttgga agatatgccc
    31561 aagattgcca gtttggcacg ctgtccttcc gcgctccggc ggtcggcata cgcgcccatt
    31621 tgcagataat gcgttgcttc cgccttgtcg ggcgttttca ttttctgcac ttctttggca
    31681 gcggcactgc gcgctttttc gatgctgccg ctgttgagga tttgttccgg ggtcggtttg
    31741 ggtgcaactt tttccttctc cgcctttttc tcttcttttg aagctttttt ctctgtttct
    31801 ttagatggtt ttaccgcttg ttttttaacc gtttcggcat ctttcttctg cgctttttcc
    31861 ccgacggttt gttcacgctc ttccgtcagt gctttcttgc gcactgcctg tccgtccgac
    31921 ttttcccgct ccggctcgtc cgccttttcc tcaacctcgt cggctttgtc ggcaacgggc
    31981 tgcttgtcgg cagctttttc cgcatccgac tgctctgcct ctttcgcagc atccggttcg
    32041 gacaaggcgt tttgatcggc cggttcaggt tggatgtctt ccttaggctg gtttttcggt
    32101 ttcaggattt ccgtttctgc aggctgcttc gacggaaccg ggattttgaa cgcattttga
    32161 ccgctctggt tcagataaaa caaaataccg gcaataatga ccgtcgccag tatcaaaccg
    32221 aagaaaaaac cggacagacc ttttccggat tgggaaaatt tgttcataaa cataccttaa
    32281 tgtgtttcag acggcattaa cgccgtttgc ttacgggcgg atattctaac aatatcgcca
    32341 tatttgggca aaacctgctt ccattcccat tcctataaag cacgacggaa acctatggct
    32401 ttcatgacgt ggcttctgcc gacttcttcg tcgcccgcca aatccgccaa tgtacgcgcc
    32461 acgcgcataa tgcggtggaa gctgcgggcg gaaagggaga gtttttccag caggccgccc
    32521 aatgcttcct gcgcttcttt ttgaatgcgg gcttgggaat cgagttcgct gacactcaaa
    32581 gcggcgttga ctttgccctg ccgcgcgtat tgtttgtcgc gggcggcgat aacgcgttcc
    32641 aaaacggacg cgctgctttc acccgcttcc tgctgcatca gttcggcggc ggacaggctg
    32701 gggacttcga tggtcaaatc gatgcggtcg agcagcgggc cggaaatttt gctgcggtaa
    32761 cgcgcgacgc tttcgggcgt gcagcggcag ggtttgacgg gatgcccgag ataaccgcac
    32821 gggcaggggt tcatggcggc aacaagttgg aatttggcag gatagacggc ttggcgcgcc
    32881 gcgcgggaaa tgtggatttc gccgttttcc aacggttcgc gcaaaacttc caaaactttg
    32941 cggtcgaact cgggcagctc gtccaaaaac aatacgccgt ggtgcgccaa tgaaatctcg
    33001 cccggacgcg ggtcggaacc gccgccgacc atagccgccg cgctggcact gtgatgcgga
    33061 ctgcggaaag gacggtggct gtcgagttgt tgctggtggt tgggcaggag cgaacgcaat
    33121 gcccaaactt caaccaattc gtcttcggtc agcggcggca ggatgcctgg cagccgttgg
    33181 gagagcatag acttgcccgt tcccggcgga cccatcatca agaggctgtg tccgcctgcg
    33241 gcagcgattt ccaaagcaag gcgggcagtg tgctgacctt tcacatcaca caaatcaggt
    33301 tgtccgccat gttcagacgg catttgcggt acggaacatt cggtttgcgc caaaggttcg
    33361 ataccgttca aatgggcggc gacttcgccc aaagagcgcg cgccgtaaac ggtaatgccg
    33421 cgcatcacgg cggcttgtcc tgcgttttct tcaggcaaaa caaatgcacg ttttgcctgc
    33481 ataccctgcc acgccatcgc caacgcgccg cgcacggggc gcaacagccc cgacagtgcc
    33541 aattcccccg caaactcgta ttccgccagt ttttcgggcg caacctgccc cgatgcggca
    33601 aggatgccga ttgcaatcgg caaatcgaaa cgccccgact ctttgggcag gtcggcgggg
    33661 gcgaggttga cggtgatttt tttggcgggg aattcaaaac cgctttgaat aatggcggca
    33721 cggacgcggt cgcgactttc tttgacttcc gtatcgggca gtccgacgat gttgaaatgt
    33781 ggcaggccgt tggcaaggtg ggcttccact tcgaccaacg gcgcattcat accgctcaag
    33841 gcgcggctgt aaaccaaggc aagcgacata tttcagacgg ccttattcgc cggcttcggt
    33901 ttgctgcctg atttcggcga cggcttcttc ggcagcggct tcagccgctt ccaatgctgc
    33961 ccgttcggga ttttgcgcgg cttcgagttt tgccaaacgc gcttccaaag ccgccagttt
    34021 ggtacgggtt ttgattaaaa cctgctgctg gatgtcgaat tcttcgcgcg taaccaaatc
    34081 catacggttg aacgcgccgc ccagcatcgc cttaatattt ttttccacat ctttggcagg
    34141 gctgttgacg atggtttcgc tgattttcga accgacttct tcaaaaagct gcttgccgaa
    34201 cataatctgt atccttcctg aacatatcaa attcaatcgg ctattgtata aggaaaaatg
    34261 ccgtctgaaa acgggcggcg gataatcggc aaaacatacc gcgcctcctt tgcggttgct
    34321 aaattacacc tcaaaacacc tgccgcctga aaacggattt catattccgc gcaacgccag
    34381 attacaagac actacaaaac aatatactgt tttaaatgat ttttccgacg cgcatcgttt
    34441 caaactcggc tttttaagcc attaagtgct ttgcaaacaa cagaaattgg gttatgctga
    34501 aacggattat ttacaatttc atatagtttt attacatatc ttattgtgat tgaagataat
    34561 ttatccgaat ccccctttcg ggtatccgga ttttccgttg tacttttatt tagaaaaaca
    34621 tttcaggcgc aagttgcttg caatttcaaa gcctgtaatt ctgaagtttt agacgaggaa
    34681 tagcacatga atcccatgta cattactttt gcaatctatc tggttgcagt ccttctcatc
    34741 gggcttgccg cctatttttc tacgcgcaat ttcgacgatt atattttggg cgggcgcagc
    34801 ctaggcccgt ttgttaccgc gatgtcggca ggcgcgtccg atatgtccgg ctggctgctg
    34861 atgggtctgc cgggcgcgat ttacctgagc ggtttgaatg aggcttggat tgccatcggc
    34921 ctcttggtcg gcgcgtattt caactggctc ttggtggcgg gccgcctgcg cgtgcatacc
    34981 gaatacgcca acaatgcgct gacgctgccg gattatttct tccaccgctt cggcgcgggc
    35041 ggacacttga tgaaagtggt ttccgcacta attatcttgt ttttcttcac aatttactgt
    35101 gcttccggca ttgtggccgg cgcaaccctg ttccaaagtc tgtttgaagg tatgacttac
    35161 aaccaggcaa tgtggctggg tgcgggcgcg accatcgtct ataccttctt gggcggtttc
    35221 ttggcagtca gctggacgga tacgctgcaa gcatctttga tgattttcgc cctgatttta
    35281 acgccggtga tggtctatct gggcttgggc ggcgcggaac agatgtctgc cgcgattcaa
    35341 agcgtcgccg caggcacggg caaagaatac ggcagcctgt ttgccggtac gaccgtcatc
    35401 ggcatcattt ccaccgccgc atggggcttg ggctatttcg gacagccgca cattttggcg
    35461 cgctttatgg cggccgaaag cgcgaaatcg ctggtatccg cacgccgcat cggcatgact
    35521 tggatggcgt tgtgccttgc gggcgcggta gcggtcggtt atttcggcat tgcgtatttc
    35581 ggtgcaaatc ccgacaaagt ttcttctatg agcggcaacc acgaacgcat cttcatcgcg
    35641 ctttccaccc tgctgttcaa cccttggatc gccggcatta ttttgagcgc gattttggcg
    35701 gcggtgatgt ccaccctgtc ctgccagctt ttggtttgct ccagcgcgat taccgaagac
    35761 ttctacaaag gctttttgcg taaaaacgcg caacagtcgg aactggtatg ggtcggccgc
    35821 ctgatggtct tggcgattgc cgtgatttcc atcctgattg cttccgatcc gaacagcaaa
    35881 gtattgggac tggtgtctta cgcgtgggca ggtttcggtg cggcattcgg cccgattgtg
    35941 attctgtccg tattgtggaa acgcatcact gcctacggcg cgttgtcggg tatggttgca
    36001 ggtgccttaa ccgtggtcgt atgggcggaa tgggtgaaaa aaccggctca agcggcaggc
    36061 gaaagcggct tgttgacgat gtacgaaatc gtcccgggct ttattgtttg cttgatcgtt
    36121 gccgtgttgg tttccctgtt caacaaagaa ccttcgcgcg aaacccaaga acgctttgaa
    36181 aaagccgacg cagattaccg cgccgcccga taaacccctt tcagacggca cggcatcccg
    36241 gttcgtgccg tctgaacccg tttccgggtc aaacacaaat aaaccttgcc tgcatacagg
    36301 ccttatttgt gttttacccg tttcattttc aaagaaacgg aagcacctac cctgcccgca
    36361 tttgaagcct tgtatttcat ctgcggattt attacgatat taccgtattc agaccgcacc
    36421 gatgccgtct gaaccccccg aaaaactttg gagaatccaa aaaatgtttc attttgcatt
    36481 tccggcacaa actgccctgc gccaagcgat aaccgatgcc taccgccgta atgaaatcga
    36541 agccgtacag gatatgttgc aacgtgcaca gatgagcgac gaagagcgca acgccgcctc
    36601 cgagcttgcc cgccgtttgg ttacccaagt ccgcgccggc cgcaccaaag ccggcggcgt
    36661 ggatgcgctg atgcacgagt tttcactctc cagcgaagaa ggcatcgcgc tgatgtgtct
    36721 ggcagaagcc ctgctgcgta tccccgacaa cgccacgcgc gaccgcctga ttgccgacaa
    36781 gatttcagac ggcaactgga aaagccattt gaacaacagc ccttccctct tcgtcaacgc
    36841 cgccgcctgg ggcctgctga ttaccggcaa actgaccgcc acaaacgaca gacaaatgag
    36901 ttccgcactc agccgcctga tcagcaaagg cggcgcaccg ctcatccgcc aaggcgtaaa
    36961 ttacgccatg cgcctgttgg gcaaacagtt cgtaaccgga cagaccattg aagaagccct
    37021 gcaaaacggc aaagaacgcg aaaaaatggg ctaccgcttc tccttcgata tgttgggcga
    37081 agccgcctac acccaagccg atgccgaccg ctactaccgc gactatgtcg aagccatcca
    37141 cgccatcggc aaagatgcgg caggacaagg cgtttacgaa ggcaacggca tttccgtcaa
    37201 actctccgcc atacatccgc gctactcgcg cgcccaacac ggccgtgtga tgggcgaact
    37261 gttgccgcgc ctgaaagagc tgttcctttt gggtaaaaaa tacgatatcg gtatcaacat
    37321 cgatgccgaa gaagccaacc gtctggagct gtctttggat ttgatggagg cactcgtttc
    37381 cgatcccgat ttggcaggct acaaaggcat cggtttcgtt gttcaagcct accaaaaacg
    37441 ttgtccgttc gttatcgact acctgatcga ccttgcccgc cgcaacaacc aaaaactaat
    37501 gatccgcctc gtcaaaggcg cgtattggga cagcgaaatc aaatgggcgc aagtggacgg
    37561 cttgaacggc tatccgacct acacccgcaa agtccacacc gacatctcct acctcgcctg
    37621 cgcgcgcaaa ctgctttccg cgcaagacgc ggtatttccg caattcgcca cccacaacgc
    37681 ctacaccttg ggcgcaatct accaaatggg caaaggcaaa gactttgaac accaatgtct
    37741 gcacggcatg ggcgaaaccc tgtacgacca agtcgtcggc ccgcaaaact taggccgccg
    37801 cgtgcgcgtg tacgccccgg tcggcacaca cgaaaccctg ctcgcctact tggtgcgccg
    37861 cctgttggaa aacggcgcga actcgtcttt cgtcaaccaa atcgtcgatg aaaacatcag
    37921 catcgacacg ctcatccgca gcccgttcga caccatcgcc gaacaaggca tccacctgca
    37981 caacgccctg ccgctgccgc gcgatttgta cggcaaatgc cgtctgaact cgcaaggcgt
    38041 ggacttgagc aacgaaaacg tattgcagca gcttcaagaa cagatgaaca aagccgccgc
    38101 gcaagacttc cacgccgcat ccatcgtcaa cggcaaagcc cgcgatgtcg gcgaagcgca
    38161 accgattaaa aaccctgccg accacgacga catcgtcggc acagtcagct ttgccgatgc
    38221 cgcgcttgcc caagaagcgg ttggcgcagc cgttgccgcg ttccccgaat ggagtgcgac
    38281 acctgccgcc gaacgcgccg cctgcctgcg ccgtttcgcc gacctgctcg aacagcacac
    38341 ccccgcgctg atgatgcttg ccgtgcgcga agcaggcaaa acgctgaaca acgccattgc
    38401 cgaagtgcgc gaagccgtcg atttctgccg ctactacgca aacgaagccg aacataccct
    38461 gcctcaagac gcaaaagccg tcggcgcgat tgtcgccatc agcccgtgga acttcccgct
    38521 cgccatcttt accggcgaag tcgtttccgc attggcggca ggcaacaccg tcatcgccaa
    38581 acccgccgaa caaaccagcc tgattgccgg ttatgccgtt tccctcatgc acgaagccgg
    38641 catcccgact tccgccctgc aactcgtcct cggcgcaggc gacgtgggtg cggcattgac
    38701 caacgatgcc cgcatcggcg gcgtgatttt caccggctcg accgaagtgg cgcgcctgat
    38761 caacaaagcc cttgccaaac gcggcgacaa tcccgtcctg attgccgaaa ccggcggaca
    38821 aaacgccatg attgtcgatt ccaccgcact tgccgaacaa gtctgcgccg acgtattgaa
    38881 ctccgccttc gacagcgcgg gacaacgctg ctccgccctg cgtatcttgt gcgtccaaga
    38941 agacgttgcc gaccgtatgc tcgacatgat caaaggcgcg atggacgaac tcgtcgtcgg
    39001 caaaccgatt cagctcacta ccgatgtcgg ccccgtcatc gatgccgaag cacagcaaaa
    39061 cctgttgaac cacatcaaca aaatgaaagg cgttgccaag tcttaccacg aagtcaaaac
    39121 cgctgccgat gtcgattccg aaaaatccac gttcgtccgc cccatcctgt ttgaattgaa
    39181 caacctcaac gaactgcaac gcgaagtctt cggtcccgtc ctgcacgtcg tccgctaccg
    39241 cgccgacaaa ctcgacagcg tcatcgacca aatcaacagc aaaggctacg ccctgaccca
    39301 cggcgtacac agccgcatcg aaggcacagt acgccacatc cgcagccgca tcgaagccgg
    39361 caacgtttac gtcaaccgca acatcgtcgg cgcagtcgtc ggcgtacagc ccttcggcgg
    39421 acacggtctg tccggcacag gccccaaagc aggcggttcg ttctacctgc aaaaactgac
    39481 ccgcatcccc gaatgggttg ccccgaccct gagccaaatc ggacaggcag acgaagccgc
    39541 gctcaaacgc ctcgaagcac tggttcacaa actgccgttc aacgccgaag agaaaaaagc
    39601 cgcagcggcc gctttgggac acgcccgcat ccgcaccctg cgccgtgccg aaaccgtcct
    39661 taccggcccg accggcgagc gcaacagcat ctcatggcac gcgccaaaac gcgtctgggt
    39721 acacggcggc agcctcctgc aagccttctg cgccctaacc gaacttgccg cctccggcgt
    39781 acaggcagtg gtcgaacccg acagcccgct ggcttcctac accgccgact tggaaggtct
    39841 gctgctggtc aacggcaaac ccgaaaccgc cgacatcagc cacgttgccg ccctgtcgcc
    39901 tttggacagc gcgcgcaaac aggaacttgc cgcccacgac ggcgcactca tccgcatcct
    39961 cccttcggaa aacggactcg acatcctgca agtgtttgaa gaaatctctt gcagcgtcaa
    40021 caccacagcc gccggcggca acgccagcct gatggcggtt gccgactgat tttaccgaaa
    40081 tccccgagcg cggcccgtga accaatgccg tctgaaaacc tttcagacgg catttttata
    40141 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggaa
    40201 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
    40261 acgccgtact ggtttttgtt aatccgctat aacag
//
LOCUS       DAXIHS010000006        39271 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXIHS010000006 DAXIHS010000000
VERSION     DAXIHS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.006"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            1..178
                     /locus_tag="ACFOF2_000233"
                     /pseudo
     CDS             1..178
                     /locus_tag="ACFOF2_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..1086
                     /gene="rplC"
                     /locus_tag="ACFOF2_000234"
     CDS             442..1086
                     /gene="rplC"
                     /locus_tag="ACFOF2_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC7106620.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1086..1706
                     /gene="rplD"
                     /locus_tag="ACFOF2_000235"
     CDS             1086..1706
                     /gene="rplD"
                     /locus_tag="ACFOF2_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC7106621.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1703..2017
                     /gene="rplW"
                     /locus_tag="ACFOF2_000236"
     CDS             1703..2017
                     /gene="rplW"
                     /locus_tag="ACFOF2_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC7106622.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2023..2856
                     /gene="rplB"
                     /locus_tag="ACFOF2_000237"
     CDS             2023..2856
                     /gene="rplB"
                     /locus_tag="ACFOF2_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC7106623.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2863..3141
                     /gene="rpsS"
                     /locus_tag="ACFOF2_000238"
     CDS             2863..3141
                     /gene="rpsS"
                     /locus_tag="ACFOF2_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC7106624.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3150..3479
                     /gene="rplV"
                     /locus_tag="ACFOF2_000239"
     CDS             3150..3479
                     /gene="rplV"
                     /locus_tag="ACFOF2_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC7106625.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3489..4181
                     /gene="rpsC"
                     /locus_tag="ACFOF2_000240"
     CDS             3489..4181
                     /gene="rpsC"
                     /locus_tag="ACFOF2_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC7106626.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4165..4581
                     /gene="rplP"
                     /locus_tag="ACFOF2_000241"
     CDS             4165..4581
                     /gene="rplP"
                     /locus_tag="ACFOF2_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC7106627.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4581..4772
                     /gene="rpmC"
                     /locus_tag="ACFOF2_000242"
     CDS             4581..4772
                     /gene="rpmC"
                     /locus_tag="ACFOF2_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC7106628.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4772..5035
                     /gene="rpsQ"
                     /locus_tag="ACFOF2_000243"
     CDS             4772..5035
                     /gene="rpsQ"
                     /locus_tag="ACFOF2_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC7106629.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5257..5625
                     /gene="rplN"
                     /locus_tag="ACFOF2_000244"
     CDS             5257..5625
                     /gene="rplN"
                     /locus_tag="ACFOF2_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC7106630.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5637..5960
                     /gene="rplX"
                     /locus_tag="ACFOF2_000245"
     CDS             5637..5960
                     /gene="rplX"
                     /locus_tag="ACFOF2_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC7106631.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5970..6509
                     /gene="rplE"
                     /locus_tag="ACFOF2_000246"
     CDS             5970..6509
                     /gene="rplE"
                     /locus_tag="ACFOF2_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC7106632.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6512..6817
                     /gene="rpsN"
                     /locus_tag="ACFOF2_000247"
     CDS             6512..6817
                     /gene="rpsN"
                     /locus_tag="ACFOF2_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC7106633.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6833..7225
                     /gene="rpsH"
                     /locus_tag="ACFOF2_000248"
     CDS             6833..7225
                     /gene="rpsH"
                     /locus_tag="ACFOF2_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC7106634.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYADRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7239..7772
                     /gene="rplF"
                     /locus_tag="ACFOF2_000249"
     CDS             7239..7772
                     /gene="rplF"
                     /locus_tag="ACFOF2_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC7106635.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7786..8139
                     /gene="rplR"
                     /locus_tag="ACFOF2_000250"
     CDS             7786..8139
                     /gene="rplR"
                     /locus_tag="ACFOF2_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC7106636.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8158..8676
                     /gene="rpsE"
                     /locus_tag="ACFOF2_000251"
     CDS             8158..8676
                     /gene="rpsE"
                     /locus_tag="ACFOF2_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC7106637.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8669..8854
                     /gene="rpmD"
                     /locus_tag="ACFOF2_000252"
     CDS             8669..8854
                     /gene="rpmD"
                     /locus_tag="ACFOF2_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC7106638.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8856..9290
                     /gene="rplO"
                     /locus_tag="ACFOF2_000253"
     CDS             8856..9290
                     /gene="rplO"
                     /locus_tag="ACFOF2_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC7106639.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9302..10612
                     /gene="secY"
                     /locus_tag="ACFOF2_000254"
     CDS             9302..10612
                     /gene="secY"
                     /locus_tag="ACFOF2_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC7106640.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10617..10835
                     /gene="infA"
                     /locus_tag="ACFOF2_000255"
     CDS             10617..10835
                     /gene="infA"
                     /locus_tag="ACFOF2_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC7106641.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10856..10969
                     /gene="rpmJ"
                     /locus_tag="ACFOF2_000256"
     CDS             10856..10969
                     /gene="rpmJ"
                     /locus_tag="ACFOF2_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC7106642.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11035..11397
                     /gene="rpsM"
                     /locus_tag="ACFOF2_000257"
     CDS             11035..11397
                     /gene="rpsM"
                     /locus_tag="ACFOF2_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC7106643.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11417..11812
                     /gene="rpsK"
                     /locus_tag="ACFOF2_000258"
     CDS             11417..11812
                     /gene="rpsK"
                     /locus_tag="ACFOF2_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC7106644.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11832..12452
                     /gene="rpsD"
                     /locus_tag="ACFOF2_000259"
     CDS             11832..12452
                     /gene="rpsD"
                     /locus_tag="ACFOF2_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC7106645.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12478..13464
                     /gene="rpoA"
                     /locus_tag="ACFOF2_000260"
     CDS             12478..13464
                     /gene="rpoA"
                     /locus_tag="ACFOF2_000260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC7106646.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13488..13856
                     /gene="rplQ"
                     /locus_tag="ACFOF2_000261"
     CDS             13488..13856
                     /gene="rplQ"
                     /locus_tag="ACFOF2_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC7106647.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14003..14716
                     /gene="minC"
                     /locus_tag="ACFOF2_000262"
     CDS             14003..14716
                     /gene="minC"
                     /locus_tag="ACFOF2_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC7106648.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSDERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14745..15560
                     /gene="minD"
                     /locus_tag="ACFOF2_000263"
     CDS             14745..15560
                     /gene="minD"
                     /locus_tag="ACFOF2_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC7106649.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15564..15827
                     /gene="minE"
                     /locus_tag="ACFOF2_000264"
     CDS             15564..15827
                     /gene="minE"
                     /locus_tag="ACFOF2_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC7106650.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVDVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15831..16751
                     /gene="oxyR"
                     /locus_tag="ACFOF2_000265"
     CDS             15831..16751
                     /gene="oxyR"
                     /locus_tag="ACFOF2_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC7106651.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16748..16939)
                     /locus_tag="ACFOF2_000266"
     CDS             complement(16748..16939)
                     /locus_tag="ACFOF2_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106652.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(17081..19918)
                     /locus_tag="ACFOF2_000267"
     CDS             complement(17081..19918)
                     /locus_tag="ACFOF2_000267"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC7106653.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHTFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(20015..20824)
                     /gene="zupT"
                     /locus_tag="ACFOF2_000268"
     CDS             complement(20015..20824)
                     /gene="zupT"
                     /locus_tag="ACFOF2_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC7106654.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(21005..22261)
                     /locus_tag="ACFOF2_000269"
     CDS             complement(21005..22261)
                     /locus_tag="ACFOF2_000269"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC7106655.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETKTGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTHFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22340..23731)
                     /locus_tag="ACFOF2_000270"
     CDS             complement(22340..23731)
                     /locus_tag="ACFOF2_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC7106656.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVLLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWITAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(24088..24864)
                     /gene="lpxA"
                     /locus_tag="ACFOF2_000271"
     CDS             complement(24088..24864)
                     /gene="lpxA"
                     /locus_tag="ACFOF2_000271"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC7106657.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKNVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
     gene            complement(24907..25587)
                     /locus_tag="ACFOF2_000272"
     CDS             complement(24907..25587)
                     /locus_tag="ACFOF2_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC7106658.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDVTGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(25794..26243)
                     /gene="fabZ"
                     /locus_tag="ACFOF2_000273"
     CDS             complement(25794..26243)
                     /gene="fabZ"
                     /locus_tag="ACFOF2_000273"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC7106659.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(26277..27323)
                     /gene="lpxD"
                     /locus_tag="ACFOF2_000274"
     CDS             complement(26277..27323)
                     /gene="lpxD"
                     /locus_tag="ACFOF2_000274"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC7106660.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27356..27856)
                     /locus_tag="ACFOF2_000275"
     CDS             complement(27356..27856)
                     /locus_tag="ACFOF2_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC7106661.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(27922..30315)
                     /gene="bamA"
                     /locus_tag="ACFOF2_000276"
     CDS             complement(27922..30315)
                     /gene="bamA"
                     /locus_tag="ACFOF2_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC7106662.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLALN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30372..31712)
                     /gene="rseP"
                     /locus_tag="ACFOF2_000277"
     CDS             complement(30372..31712)
                     /gene="rseP"
                     /locus_tag="ACFOF2_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC7106663.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(31747..32982)
                     /gene="ispC"
                     /locus_tag="ACFOF2_000278"
     CDS             complement(31747..32982)
                     /gene="ispC"
                     /locus_tag="ACFOF2_000278"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC7106664.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(32987..33784)
                     /locus_tag="ACFOF2_000279"
     CDS             complement(32987..33784)
                     /locus_tag="ACFOF2_000279"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC7106665.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(33787..34533)
                     /locus_tag="ACFOF2_000280"
     CDS             complement(33787..34533)
                     /locus_tag="ACFOF2_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC7106666.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34589..35146)
                     /gene="frr"
                     /locus_tag="ACFOF2_000281"
     CDS             complement(34589..35146)
                     /gene="frr"
                     /locus_tag="ACFOF2_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC7106667.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35326..36453)
                     /locus_tag="ACFOF2_000282"
     CDS             complement(35326..36453)
                     /locus_tag="ACFOF2_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC7106668.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36527..36602)
                     /locus_tag="ACFOF2_000283"
     tRNA            complement(36527..36602)
                     /locus_tag="ACFOF2_000283"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36567..36569),aa:Thr,seq:cgt)
     gene            36709..37181
                     /locus_tag="ACFOF2_000284"
                     /pseudo
     CDS             36709..37181
                     /locus_tag="ACFOF2_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37277..37900
                     /gene="rsmG"
                     /locus_tag="ACFOF2_000285"
     CDS             37277..37900
                     /gene="rsmG"
                     /locus_tag="ACFOF2_000285"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC7106669.1"
                     /translation="MERKERLQSGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDHLPQDVCVEKVQRLDVPGLDAERHIVILRKR"
     gene            37999..38772
                     /locus_tag="ACFOF2_000286"
     CDS             37999..38772
                     /locus_tag="ACFOF2_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC7106670.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT         9222 a         8892 c        10773 g        10384 t
ORIGIN      
        1 ccgttacctc cgagattacc gcggccgtcc cgcctacccg ctgttgtcca tgttcaaagc
       61 cgtcctgctc ggacaatggc acagcctctc cgatcccgaa ctcgaacaca gcctcatcac
      121 ccgcatcgat ttcaacctgt tttgccgctt tgacgaactg agcatccccg attacagtca
      181 tcaaccatat tccggtttgt tggagaaaga tgcatacgct gtgatgaccg gataccgacc
      241 ctttaaaaga gtctgaccct atgccgtctg aaaattcaaa acgcttcaga cggcatattg
      301 aagatatttc tgatatttct gttgatattt ctttaacttg tcagatataa tgccgagctt
      361 ggtacatttg tgccaagttt aactttgtct gaaagacagg ccaatcgtag cctgtccctt
      421 tactttaaaa ggaaaataat catgacttta ggtctggttg gacgcaaagt tggtatgacc
      481 cgcgtgttcg acgaacaggg tgtttctgtt ccggtaaccg ttttggatat gtctgccaac
      541 cgcgttacac aagtaaaatc caaagatact gacggctata ctgccgttca agttaccttt
      601 ggtcagaaaa aagccaatcg tgtcaacaaa gccgaagccg ggcactttgc aaaagcaggt
      661 gttgaagccg gtcgcggttt gattgagttt gctttgactg aagaaaaact ggctgaattg
      721 aaagctggtg acgaaatcac cgtttctatg tttgaagtcg gtcaactggt cgatgtaacc
      781 ggtacctcta aaggtaaagg tttctccggc acgattaaac gtcataactt cggcgcccaa
      841 cgtacttccc acggtaactc ccgctctcac cgcgttccag gttctatcgg tatggcacaa
      901 gacccgggtc gcgtgttccc cggtaagcgc atggccggcc aatacggcaa caccaaggca
      961 actgttcaaa aattggaagt tgtccgtgtt gatgcagaac gccaactgct gttggttaag
     1021 ggtgctgttc cgggtgcggt caacagcgat gttgtagttc gtcccagcgt gaaagtaggt
     1081 gcgtaatgga attgaaagta attgacgcta aaggacaagt ttcaggcagc ctgtctgttt
     1141 ctgatgcttt gttcgcccgc gaatacaatg aagcgttggt tcatcagctg gtaaatgcct
     1201 acttggcaaa cgcccgctcc ggtaaccgcg ctcaaaaaac ccgtgccgaa gtaaaacact
     1261 caaccaaaaa accatggcgt caaaaaggta ccggccgtgc ccgttccggt atgacttctt
     1321 ctccgctgtg gcgtaaaggt ggtcgcgcgt tcccgaacaa acccgacgaa aacttcactc
     1381 aaaaagtaaa ccgcaaaatg taccgtgccg gtatggcgac tattctgtcc caattgactc
     1441 gtgacgagcg tttgtttgcg attgaggcgt tgactgccga aactcctaaa accaaagttt
     1501 ttgctgaaca agtgaaaaat ctgggtctag agcaagtgtt gtttgtaacc aaacagctcg
     1561 acgagaatgt ttacttggct tcacgcaact tgccaaacgt gttggttttg gaagctcaac
     1621 aagttgatcc ttacagcttg ctgcgttaca aaaaagtaat catcactaaa gatgcagttg
     1681 cacaattaga ggagcaatgg gtatgaatca acaacgtttg actcaagtga ttttggcacc
     1741 tatcgtttct gaaaaaagca acgtattggc tgaaaaacgc aaccaaatga cgtttaaagt
     1801 tttggcaaat gcaaccaaac ctgaaatcaa agcggctgtt gagctgctgt ttggtgttca
     1861 agttgcttct gtaactaccg ttacaactaa aggcaaaact aagcgttttg gtcgtacttt
     1921 gggccgccgc agcgatgtta aaaaagctta tgtaagcttg gctgccggtc aagagttgga
     1981 tttggaagcc gctgctgcag ctgcagataa ggaataaaca aaatggcaat cgttaaaatg
     2041 aagccgacct ctgcaggccg tcgcggcatg gttcgcgtgg taacagaagg tttgcacaaa
     2101 ggtgcgcctt atgcaccttt gctcgaaaag aaaaattcta ctgccggtcg taacagtaat
     2161 ggtcatatca ccacccgtca caaaggcggc ggtcataaac accattaccg tgttgtagac
     2221 tttaaacgta acaaagacgg cattcctgct aaggtagagc gtatcgaata cgatcctaac
     2281 cgtactgcct tcatcgcact gttgtgctat gcagacggcg agcgtcgtta catcatcgct
     2341 cctcgcggta ttcaagtcgg tgccgtattg gtttccggtg ctgaagctgc catcaaagta
     2401 ggcaacaccc tgccgatccg caacattccc gttggtacga ctatccactg tatcgaaatg
     2461 aaacccggta aaggtgctca aatcgcacgt tctgccggtg cttctgcggt attgctggct
     2521 aaagaaggcg cgtacgctca agtccgtctg cgctctggcg aagttcgtaa aatcaacgta
     2581 aattgccgtg cgaccatcgg tgaagttggt aacgaagagc aaagcctgaa aaaaatcggt
     2641 aaagccggtg ccaaccgttg gcgcggtatt cgtccgaccg tacgcggtgt tgtcatgaat
     2701 cctgtcgatc acccgcatgg tggtggtgaa ggccgtacgg gcgaggcccg cgaaccggtc
     2761 agcccatggg gtactcctgc taaaggctac cgcactcgta ataacaaacg cacggataac
     2821 atgattgttc gtcgccgtta ctcaaataaa ggttaattta gtatggctcg ttcattgaaa
     2881 aaaggcccat atgtagacct gcatttgctg aaaaaagtag atgctgctcg cgcaagcaac
     2941 gacaaacgcc cgattaaaac ctggtctcgt cgttctacca ttctgcctga ttttatcggt
     3001 ctgaccattg ctgtgcacaa cggccgcacc catgtgcctg tgtttatcag cgacaatatg
     3061 gttggtcata aattaggcga attctcattg acccgtacct ttaaaggcca cttggccgat
     3121 aaaaaggcta aaaagaaata aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc
     3181 gtatctctgc tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc
     3241 aagctttgaa tattttggct ttcagtccta aaaaaggtgc cgagctgatt aaaaaagtat
     3301 tggagtcagc tattgctaat gccgagcaca ataacggtgc ggacattgat gaactgaaag
     3361 tggtaactat ctttgttgac aaaggcccaa gcttgaaacg ttttcaagct cgcgccaaag
     3421 gtcgcggtaa ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag
     3481 gaaaagctat gggacaaaag attaacccta caggctttcg cctggcggta actaaagact
     3541 gggcttcaaa atggtttgct aaaagcaccg acttttctac tgttttgaag caggatatcg
     3601 atgttcgcaa ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga gtggttattg
     3661 aacgccctgc aaaatctgca cgcattacca ttcactccgc tcgtccgggt gtggttatcg
     3721 gtaaaaaagg tgaggatatc gaggttttga aacgtgactt gcaagtcttg atgggtgtac
     3781 ctgttcatgt aaatattgaa gagattcgcc gtcctgagtt ggatgctcaa attattgctg
     3841 acggtattgc ccagcagttg gaaaagcgcg ttcaattccg tcgtgctatg aaacgagcaa
     3901 tgcaaaatgc aatgcgttct ggtgctaaag gcattaagat tatgacttca ggccgtctga
     3961 atggtgcgga tattgcccgt agcgaatggt atcgtgaagg tcgtgtgcca ctgcatactt
     4021 tacgtgcaaa tgtagattat gcaaccagcg aagcgcacac cacatatggt gtattgggtc
     4081 tgaaagtttg ggtttatacg gaaggcaata ttaaatcttc caaacctgaa catgagagta
     4141 aacaaagaaa ggcaggtaga cgtaatgctg cagccaacta gactgaaata ccgtaagcaa
     4201 caaaagggtc gcaatactgg catcgctact cgcggtaata aggtaagttt cggtgagttc
     4261 ggcttgaaag ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt
     4321 gcaatgaccc gtcatatcaa acgtggtggt cgtatttgga ttcgtgtatt ccctgataaa
     4381 ccgattactg aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat
     4441 tacattgccg aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa
     4501 ctggctcgtg aagcattcga gttggctgct gccaaattgc ctattcctac aacctttgta
     4561 gtaagacagg tgggtcaata atgaaagcaa atgaattgaa agacaaatcc gttgagcagt
     4621 tgaatgcaga tttgttggac ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta
     4681 ccggtcaatt aggcaaacca agtgaattga aacgtgtacg tcgcgatatt gctcgtatta
     4741 aaaccgtttt aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt
     4801 gcaaggcaaa gtagtaagcg acaaaatgga taaaaccgta acagtattgg ttgagcgtaa
     4861 agtaaaacat ccgctgtatg gtaagattat tcggttatct actaaaatcc atgcccatga
     4921 tgaaaataat caatatggaa ttggtgatgt ggttgttata tcggaatccc gtccattgtc
     4981 aaaaactaaa tcttgggttg tcagtgagct ggttgagaaa gcacgttcta tttaagaatt
     5041 aaagcaacgt gcttggaatg ggaaacgaag tattgcagca aatttaattt gcgtgtaaac
     5101 ttcgtttcct gtctttcagt ttcttctgga agtttcttcc ctttcggggt ccaagactgg
     5161 tttacttgaa ccgcaaggtt tcatttaata agcagcggct ttgctgtaag ttatctgaaa
     5221 gtggtaaatt aagttggtta atttaaaggt aataacatga ttcaaatgca gaccatctta
     5281 gatgtggctg ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt gggcggatct
     5341 aagcgtcgct acgcttctgt tggcgatatt attaaagtgg cagttaaaga tgcggctccg
     5401 cgtggccgtg tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac tgctaagggt
     5461 gtacgtcgtc ctgatggtgc gttaattaaa ttcgataaca atgccgccgt gttactgaat
     5521 aataaacttg aacctttggg tactcgtatc tttggtccgg taacccgtga attgcgtact
     5581 gagcgattta tgaaaatcgt ttcattggca cctgaagtat tataaggaat ggcacgatga
     5641 ataaaatcat taaaggcgat agggttgtag taattgctgg taaggataaa ggtaagcagg
     5701 gtcaagtagt tcgagtgttg ggtgataaag ttgttgttga gggcgttaat gttgtaaaac
     5761 gccatcaaaa acctaatcca atgcgtggca ttgagggcgg tattattact aaagaaatgc
     5821 ctttggatat ttctaatatc gcaatcctga atccggaaac taataaagcg gaccgtgttg
     5881 gtattaagct gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc aaatcaaatg
     5941 gctctatcat tggggcataa ggagataaca tggctcggtt gagagagttt tataaagaga
     6001 cagttgttcc tgaattggtt aaacaatttg gttacaaatc agtaatggaa gtcccgcgta
     6061 ttgaaaaaat caccttgaat atgggtgtgg gtgaggctgt tgctgacaaa aaagttatgg
     6121 aacatgctgt ttccgattta gagaaaattg ccggtcaaaa accggttgtt actgttgccc
     6181 gtaaatctat cgcaggtttt aaaatccgtg ataactatcc ggttggttgc aaagtaacat
     6241 tgcgtcgtga tcaaatgttt gaattcttgg atcgtttgat tactattgca ttacctcgcg
     6301 tacgtgactt ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat tacaatatgg
     6361 gtgttcgtga gcaaattatt tttccggaaa ttgaatacga taaaattgat gctttgcgtg
     6421 gtttgaatat tactattact actacagcaa aaaccgatga ggaagcgaaa gctttattgt
     6481 cattgtttaa atttccgttc aaaggataat catggctaag aaagcactta ttaatcgtaa
     6541 tctgaaacgt caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa ttaaagcggt
     6601 aatcaatgat tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga ggtttcaatc
     6661 cattcctcgt aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga caggtcgccc
     6721 tcgtggtact ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa tcgccatgcg
     6781 tggcgaaatt ccgggtgttg ttaaagccag ctggtaatag gagtaattaa gaatgagtat
     6841 gcatgatcct atttccgata tgttgactcg tatccgcaat gcgcaacgtg ctaataaagc
     6901 agcggttgca atgccttctt caaaattaaa gtgtgctatt gcaaaggtat tgaaagaaga
     6961 aggatatatt gaggacttcg cagtttcatc tgacgtaaag tctatattgg aaattcaatt
     7021 aaaatactat gcagatcgtc ctgtaattga acaaatcaag cgtgtatctc gccccggttt
     7081 gcgtatttat aaagcgtcta gtgagattcc aagtgttatg aatggcttgg gtattgctat
     7141 tgttagtact tctaaaggtg taatgactga tcgtaaagca cgttctcaag gtgttggtgg
     7201 tgagttgtta tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc gcaaaaaacc
     7261 cagtgactgt tcccgctggt gtagaagtaa aatttggaac agaggcatta gttattaagg
     7321 gtaagaacgg tgaattgtct tttcctttgc attctgatgt agccattgaa tttaatgatg
     7381 gcaaattggc ttttgttgcg aataacagca gtaaacaagc aaatgcaatg tctggtactg
     7441 ctcgcgcatt agtcagcaat atggttaaag gtgtttcaga aggttttgag aaaagattgc
     7501 aattgatagg tgtgggttat cgtgctcaag cacaaggtaa aatcttgaat ctgtctttgg
     7561 gtttttctca tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa actcctagcc
     7621 aaacagagat tgttttaacc ggctcggata aacaagttgt tggtcaagtt gctgctgaga
     7681 ttcgtgcgtt ccgtgctcct gagccttata aaggtaaagg tgttcgctat gtaggagaag
     7741 tagtggtaat gaaagaagcc aagaaaaaat aattgaggtt cactaatgga taaacataca
     7801 acccgactcc gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa aatggtaaga
     7861 ttatgtgtgt tccgaagcaa taatcatatt tatgctcaag taattagtgc tgaaggtgat
     7921 aaagtattgg ctcaagcctc tacattggaa gctgaggtgc gcggtagtct gaaatctgga
     7981 agcaatgttg aagcagctgc aatagttggt aaacgtatcg ctgagaaggc taaagcagca
     8041 ggtgtagaaa aggttgcttt tgatcgttca ggtttccaat atcacggtcg tgtgaaggct
     8101 ttggctgaag ctgctcgtga aaatggttta agcttctaaa tatttggaga ctttcagatg
     8161 gcaaaacatg aaattgaaga acgcggtgac ggtctgattg aaaagatggt cgcagttaac
     8221 cgtgtaacca aagtagttaa aggtggtcgc attatggctt tctctgcgct aactgttgtt
     8281 ggtgatggag atggtcgtat tggtatgggt aaaggtaagt caaaagaagt gcctgttgca
     8341 gtccaaaagg cgatggatca agcacgacgc tctatgatta aggtaccatt aaaaaatggt
     8401 acgattcatc atgaggttat tggtcgacat ggtgctacta aagtatttat gcagcctgct
     8461 aaagagggta gtggcgtaaa agccggtgga cctatgcgtt tggtttttga tgctatgggc
     8521 attcataata tctccgccaa agtgcacgga tctactaacc catataatat cgtacgtgca
     8581 acattagatg gtttgtctaa gttgcatact cctgctgata tcgcagccaa acgtggcttg
     8641 acagtggaag acattttggg agttaaccat ggctgaacaa aaaaagatta gggttacatt
     8701 ggttaaaagc ctgattggta caattgaatc tcatcgtgca tgtgcacgcg gtttaggttt
     8761 gcgtcgtcgc gagcatacgg tagaggtttt agatacccct gaaaaccgtg gtatgattaa
     8821 taaaatcagc tacttgttga aagtggagtc ttgatatgtt tttgaataca attcaacctg
     8881 ctgttggtgc tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt ggtcttggca
     8941 aaacgggtgg tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt cataaggtgg
     9001 gtttcgaggg tggtcaaatg cccttgcaac gacgcctccc taaaagaggt tttaaatctt
     9061 taacagcatc agctaatgca cagcttcgtt taagtgaact ggaatcaatt gctgttaatg
     9121 agattgatat tttggtctta aagcaagcgg gtctgattgc atctacagtc tctaatgtta
     9181 aagttattgc ttctggtgaa atttctaagg cagttgcttt gaagggtatt aaagttacca
     9241 aaggtgcgag agctgctatc gaggctgttg gtggtaagat tgaaatgtaa ggtttaatat
     9301 tgtggctaat caacaaacgt catcaggttc atccaaattt ggagatctta agaaacgtct
     9361 tttgtttcta tttggagcat tgattgtttt tcgaattggt gcccatatac ccgtacctgg
     9421 agttgatgct gttgctttag ctaaattata cgaaagcgct ggaaacggca tcctgggaat
     9481 attgaatatg ttttccggtg ggtcgttaga gcgctttagt atatttgcaa taggaattat
     9541 gccatatatt tcagcttcta ttattgtaca gctcgcttct gaaattttgc catcattgaa
     9601 ggctttaaaa aaagaagggg aggctggtag aaaggtaatt acgaaatata ctaggtatgg
     9661 tactgttttg ttagcaattc ttcaaagtct aggtgttgca tctttcgtat ttcagcaagg
     9721 aattgttgta acaagttcat ttgagtttca tgtttccacg gtagtttctt tggtaacggg
     9781 aaccatgttt cttatgtggc ttggggagca aattactgaa aggggtatcg ggaacggtat
     9841 ttctttaatc attacggcag gtattgcttc aggtattcct tcgggtattg caaagctggt
     9901 tacactgacg aaccaaggtt ctatgagcat gcttacggcg ttgtttattg tatttggtgc
     9961 cttattatta atttatttgg ttgtatactt tgaaagtgca cagcggaaga ttcctattca
    10021 ttatgcaaaa cgccagttta atggtagggc gggtagtcaa aatacgcata tgcctttcaa
    10081 gttgaatatg gctggtgtta ttcccccaat ttttgcttcc agtattattc tatttccatc
    10141 tactctttta ggttggtttg gttcggctga tacaaatagt gttttgcaca aaatagctgg
    10201 attgttacaa cacggtcaat tgctgtatat ggctttattt gcagcgacag ttattttctt
    10261 ttgttatttt tatacggctt tggtttttag ccctaaagaa atggcagaga atttaaaaaa
    10321 gagtggtgct tttgttcctg ggattagacc tggtgagcag acctctaggt atttagaaaa
    10381 agttgtatta cgtttgacat tgtttggagc tctttatatt acaactattt gtttaattcc
    10441 agagttctta actacggttt taaatgtacc tttttatttg ggtggcacgt ctttgttgat
    10501 tctagttgtt gtaacgatgg attttagtac acaaataaat tcgtataggc ttactcaaca
    10561 gtatgataag ttaatgactc gttcagaaat gaaatcattt tctcggaaat agaattatgg
    10621 cgaaagaaga tactatccaa atgcaaggtg aaattcttga aactttacct aatgcaacat
    10681 ttaaagtaaa acttgagaat gaccatattg tattgggtca tatttctggg aagatgcgga
    10741 tgcattacat tcgtatttct ccgggagata aggtcacagt agagctgaca ccttatgatc
    10801 taactagggc tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa ataaaatgcg
    10861 tgtacaacca tctgttaaga aaatttgccg aaattgcaag attattcgtc gaaatcgtgt
    10921 agttcgtgta atttgtactg atctccgtca caaacagcgt caaggttaat ggaatatttc
    10981 ttttaatgtg attctgtgat atagtgacac actttgccct aaaaaggaaa aaatatggct
    11041 cgtattgcag gggtaaatat ccctaataac gcacacatcg taattggtct tcaggctatt
    11101 tacggtattg gtgctactcg tgctaaattg atttgtgagg ctgcaaatat tgcgcctgat
    11161 actaaagcaa aagatttgga cgagactcaa ttagatgctt tgcgtgacca agttgccaag
    11221 tatgaagtag aaggtgattt gcgtcgtgag gtaactatga gtatcaagcg attgatggac
    11281 atgggctgct atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg tcaacgcact
    11341 cgtacaaatg cgcgtacccg caaaggtccg cgtaaagcga ttgctggtaa gaaataaatt
    11401 ttaaggaatt ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta aaaaagtacg
    11461 taaaaccgtg agtgagggta ttgtgcacgt tcatgcatct ttcaacaata ccatcattac
    11521 aatcactgac cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg ctggttttaa
    11581 aggttctcgt aaaagtacac catttgcagc acaagttgca gcagaagcag ctggtaaagt
    11641 tgcccaagag tatggcgtta aaaatttaga ggttcgtatt aaaggtccag gtccaggtcg
    11701 tgaatcctct gtacgtgctt tgaatgctct tggtttcaag attaccagca ttactgacgt
    11761 taccccgttg cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt aatattggag
    11821 tgatttgaaa catggcacgt tatattggcc ctaaatgtaa gttggcacgt cgcgaaggta
    11881 cggatttgtt tttgaagagt gcgcgccgct ctttggattc taaatgtaaa attgattccg
    11941 ctcccggtca gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg cagttgcgtg
    12001 aaaaacaaaa aatccgccgt atttatggcg tattagaacg tcagttccgt cgttatttcg
    12061 cagaagctga tcgtcgtaaa ggttctaccg gcgagttgct gttgcagttg ctggaatctc
    12121 gtttggataa tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa gcaagacagc
    12181 ttgtttctca taaggcgata gttgtgaatg gacaagttgt caatattcct tctttccaag
    12241 tgaaagctgg tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta cgtattcaag
    12301 aagcattggg tttggcaact caaatcggct tgccgggttg ggtttctgta gatgcggata
    12361 aacttgaggg tgtgttcaaa aacatgccgg atcgctcgga attgaccggt gatattaatg
    12421 aacagctggt ggtagagttc tactctaaat aatgctagct cagtgaggga cagttaaatg
    12481 cagaatagca caaccgaatt tttgaaacct cgtcaaattg atgtaaatac tttttctgca
    12541 actcgtgcaa aagtatctat gcagccattt gaacgtggtt tcggtcatac cttaggtaat
    12601 gctttgcgcc gtatcttact gtcatccatg aatggttttg ctcctactga agtagctatt
    12661 gccggtgtat tacacgaata ttctactgtt gatggtattc aggaagatgt tgttgacatt
    12721 ttgctgaata ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt tcaacttgtg
    12781 ttgaagaaat caggttcagg tgtcgtatct gccggtgata ttgagttgcc gcatgatgta
    12841 gaaattctga atcctggtca tgtcatttgt catttggctg ataacggtca aattgagatg
    12901 gaaattaaag tagagcaagg tcgtggttat caatctgttt caggtcgtca ggtagttcgt
    12961 gatgagaacc gtcagattgg tgcaatccag ttggatgcga gcttttcgcc catcagccgt
    13021 gttagctttg aggttgaacc tgcacgtgta gagcagcgga cggatcttga taagttggtt
    13081 ttggatatcg aaaccgacgg ttctattgat cctgaggaag ctgtacgcag tgcggcacgt
    13141 attttgattg atcagatgtc tatttttgct gatttgcagg gtacgcctgt ggaggaggtt
    13201 gaagaaaaag cacctcctat cgaccctgtt cttttgcgtc cggtggatga tctggaattg
    13261 acagtacgtt cagctaattg tttgaaagct gaggatattt attatattgg cgatttgatt
    13321 caacgcactg aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc tttgaatgag
    13381 attaaggaag tattggcatc taaaggtttg acactgggtt ctaagttgga agcatggcca
    13441 cctgtaggct tggaaaagcc ttaatgaaga attaaaggat aattgatatg cgtcatcgta
    13501 atggcaatcg caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg cgtaatatgg
    13561 cgaattcatt attgactcac gaaactattg taacaactct gcctaaggcc aaggaattgc
    13621 gccgtgtagt agagccgttg attacattgg gtaaaaagcc gtcattggca aaccgccgtt
    13681 tggcatttga ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc gatttgggtc
    13741 ctcgttttac tgctcgtaac ggtggttatg ttcgggtgtt gaaatacgga ttccgtaaag
    13801 gtgataatgc acctctggca ctggttgaat tggttgacaa accggctgct gagtaatttt
    13861 agtcatataa cgccatctgc cgaaaagcag gtggcgttat ttttgcaata tctgataggt
    13921 aatagggtat tggctatcat gtttaaaata ttaattgaat agctaaggtt tgcgcggtaa
    13981 acttacatca ttaaaaaatt ctatgatggt ttatataatg aatgccttcg atataaagtc
    14041 gacaaagatg gacgtattgt ctatatcttt gcatacatca gacttgtttg atttggaaga
    14101 tgtgctggtc aaattgggca agaagtttca agagtctggt gttgttccat ttgttctgga
    14161 tgttcaagag tttgattatc ccgagtcttt ggatcttgct gcattggttt cgttgttttc
    14221 aaggcatggt atgcaaattt tgggtctgaa gcattctgat gaacgttggg ctgctgcggc
    14281 tatgaagtat catttgctgt tttgtctgtc tcattcggaa aatgttaaag aactgggtca
    14341 ggttgaggtg cagaaaacgg aggatggtca gaaagcaagg aaaacagtat tgattacatc
    14401 ccctgtccgt accggtcagc aggtttatgc cgaagatggc gatttgattg ttacgggggc
    14461 ggtcagccag ggggcagaat tgattgcgga tggcaatata catatttatg cgccgatgag
    14521 ggggcgtgct ttggccggcg ccaagggtga tacttctgcc cgcatattta tccactccat
    14581 gcaggcagaa ctggtttctg tggcgggtat ttaccgtaat tttgaacagg atttgccgga
    14641 ccatctgcac aagcagccgg tacagatatt gttgcaggat aaccgattgg ttatcagtgc
    14701 aattggctca gagtaattgt ttgatatttt aaaaaggaaa tattgtggca aaaattattg
    14761 tagtaacttc aggtaagggc ggtgtcggta aaacgactac cagtgccagt attgcgacag
    14821 gtttggcatt acgcggatat aaaactgcgg taattgattt tgatgtgggt ttgcgtaacc
    14881 tcgacctcat tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat gtcattcagg
    14941 gggaggcgac gctcaaccag gctttgatta aagataaaaa ttgtgaaaac ctgtttattt
    15001 tgccggcttc ccagactcgg gataaagacg ctttgacacg cgagggcgta gaaaaagtga
    15061 tgcaggagct gtccggcaag aaaatgggct ttgagtatat tatttgcgac tctcctgccg
    15121 gtattgagca gggtgcattg atggcgttgt attttgctga tgaagccatt gtaacgacca
    15181 atcccgaggt ttccagtgtg cgtgactccg acaggatttt gggaattttg caaagcaaat
    15241 cccataaggc agagcaaggc ggttcggtta aagaacatct gttgattacg cgttattctc
    15301 ccgaacgtgt ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat attctgcata
    15361 ttcctttgct gggtgtgatt cccgaatccc aaaacgtctt gcaggcatcc aattccggag
    15421 aaccggtcat ccatcaggac agcgtggcgg cttccgaggc atataaggac gttattgccc
    15481 gtcttttggg cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa agcttcttca
    15541 aacgtctgtt cggaggatga ggtatgtcat taatcgaact tttattcggc agaaagcaga
    15601 aaacggcaac cgttgcccgc gaccgccttc aaatcatcat tgcccaagag cgcgcccaag
    15661 aaggtcaggc tccggattac ctgccgactt tacgtaaaga gttgatggaa gtcctgtcca
    15721 aatatgtgga tgtttcatta gacaatatcc gtatttccca ggaaaaacag gatggtatgg
    15781 atgtgcttga gttgaacatt actttgccgg aacagaaaaa ggtataggac atgaccttaa
    15841 ccgaattgcg gtacatcgtc gcagtcgccc aagaacgtca tttcggcagg gcggcgcggc
    15901 gttgttttgt cagccagccc actttgtcta ttgccattaa gaaattggaa gaagagcttg
    15961 ccgtctcttt gtttgaccgg agcagcaacg atattattac gaccgaggcg ggggaacgta
    16021 tcgttgcaca ggcgcgtaag gtgttggaag aggcagagct tatcaggcat ttggcaaatg
    16081 aagaacaaaa cgagctggag ggtgcgttca aactcgggct gatttttacg gttgcgccgt
    16141 acctgctgcc gaaactgatt gtctcgttgc gccgtactgc accgaaaatg cctttgatgt
    16201 tggaagagaa ttacacgcat actttgaccg agtcgctcaa acgcggggac gttgacgcga
    16261 ttatcgttgc cgaaccgttt caagagccgg gcattgttac cgaacccttg tatgacgaac
    16321 cgtttttcgt gattgtcccg aaagggcatt catttgagga actggatgcc gtttcgccac
    16381 ggatgctggg tgaggagcag gttttgctgc tgacggaagg caactgtatg cgggatcagg
    16441 tactctcaag ctgttccgaa ttggcggcga aacaacgcat acaggggttg accaatacat
    16501 tgcagggcag ctcgatcaat acaatccgcc atatggtcgc cagcggtttg gcaatcagcg
    16561 tgttaccggc aaccgcgctg accgaaaacg atcatatgct gttcagcatt attccgtttg
    16621 aaggtacgcc gccaagccgg cgggtcgtat tggcgtaccg ccgcaatttt gtccgtccga
    16681 aggcgttgtc ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg gtaagtttta
    16741 tccgcgacta ggcgcaggca ttgttttcaa aacgccattt ccctgagccg acaacacggt
    16801 atgccaagat attgccgtca tcatcgattt tgagtatagc atcgccacgg aaactgccgt
    16861 cctgaagata ttcgactttt gcatcactgt gaatgttttc atcagtgccg atgcaatgcc
    16921 atgtatagtg gattaacaaa aaccagtacg gcgttgcctc accttgccgt actatttgta
    16981 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa agaggccgtc
    17041 tgaaaaacat ttttcagacg gccttgttga ttcaatcaaa tcagtctttc aacttcgaca
    17101 actgagtttg cacttttgcc attttgtctt ccaattccgc caaatcggct ttgtcttttt
    17161 ccaccaaatg cgccggggct ttttcggtgt agccgggttt ggagagtttg gcgttgagtt
    17221 tgtccaaggc tttttgcagc ttctcggctt ctttgctcaa acgggcggtt tcggcagctt
    17281 tgtcgatttc gactttcaac atcaggcgcg cgccgttgca gacggcgacg ggcgcgtctt
    17341 cgctttcggg tagggcggcg acttgctgtg cttcggtcag gcgggtcatc atcggcaggt
    17401 atttgaggta gtccgccaag tcgtctgcgc tttcgacaaa cagcggggct ttcacgttgg
    17461 gctggatgcc catttcgccg cgcaggttgc ggactgcgcc gatcaaatcc tgcaacacgg
    17521 tcatttgccc gaatgccgtc tgaacaatct cgccgccgtc ggcttcgggg aagcgggcga
    17581 gcatgatgct gtcggcggtt ttcgcgtcgc acataggggc gacggtttgc cacagttctt
    17641 cggtgatgaa cgggataatc gggtgcagca ggcgcagggc ggcttcgagt acgcgcaata
    17701 aggtatggcg tgtggcgcgt tggcggctgg cgcagccggt ttgaagctgc actttggcaa
    17761 gttccaaata ccagtcgcaa tagtcgttcc acacgaagct gtacagggtt tccgccgcca
    17821 aatcaaagcg gtaggtttcg taggcttgcg taacctgctc gatggtctga ttcagacggc
    17881 ctacaatcca catatcgggg aaggaatagc cgcgcggttc ggtggcggtt gcgccgtagc
    17941 cgcagtcttg gttttcggtg ttcatcaaga cgaagttggt ggcgttccag attttgttgc
    18001 agaagttgcg gtagccttcg gcgcgtttga agtcgaagtt cacgctgcgc cccaagctgg
    18061 cgtagctcgc catggtgaaa cgcaatgcgt ccgcgcccat actcggaatg ccttcgggaa
    18121 agagtttttt ggtggcttct tccactttcg gcgcggtttc gggtttgcgc agaccggtgg
    18181 tacgtttcac cagcagtttt tccaagtcga tgccgtcgat caaatccaca gggtcaatga
    18241 cgttgccttc ggatttggac atttttttgc cttcgtggtc gcgcacgatg ccgtggatgt
    18301 acacgtcttt aaacggtact ttgccggtaa agtgggtggt catcataatc atgcgcgcca
    18361 cccagaagaa gatgatttcg tagccggtga ccaagacgtt ggacggcagg aaggctttga
    18421 gttcgtcggt ttcagacggc cagccgagcg tggagaacgg cacgagtgcg gaggagaacc
    18481 atgtgtccaa tacgtcttct tctcgggtca agcctgtttt gccggcttgt ttttcggctt
    18541 cttcctgatt gcgggcaacg tacacattgc cttcgttgtc gtaccatgca gggatttgat
    18601 gcccccacca cagttggcgc gagatgcacc agtcttggat gttgttcatc cattggttgt
    18661 aggtgttgac ccagttttcg gggataaagc gcaccgcgcc gctgtcgacg gcttttttgg
    18721 ctttgtcagc aaggcttaag cctttgaatt cgctgtcagg ctcgccgccg tttggcgtgg
    18781 cggacatggc gacaaaccat tggctggtca gcatcggctc aatcacggaa ccggtacggt
    18841 cgcctttcgg cgtcatcagc gtgtgcgctt tgatttcgac caagaaacct tgttcctgca
    18901 aatcggcaac catttgtttg cgcgcggcaa agcggtccaa gcctgcgtat ttttcaggca
    18961 gggcaaagct tggttgcgct tcgcctttga agttgaacac ttcggcgttt gccaatactt
    19021 tggcttccaa gtcgaacaca ttaaccaagc gcgtatcgtg gcgtttgccg acttcgtagt
    19081 cgttgaagtc gtgcgcaggc gtgattttca cgcagccggt gccgaagtct ttttcaacgt
    19141 attcgtcggc aatcacggga atggtgcggc cggtcagcgg caggattaat tctttgccga
    19201 ttaagtgggt ataacgttcg tcttcgggat tgacggcaac ggcaacgtcg cccaacagcg
    19261 tttcgggacg ggtggtggcg acgatgacgg cttcggcagg attgtccgcc agcggatagc
    19321 ggatgtgcca catagagcct tgttcttcca cgctttccac ttccaaatca gacactgcgg
    19381 tacccaatac cggatcccag ttcaccaagc gtttgccgcg ataaatcaag ccctgttcat
    19441 acaggcgtac gaacacttcg gttacggttt cggcgcgtac atcgtccatt gtgaaatact
    19501 cgcgcgtcca gtcggcagaa cagcccacac ggcgcatctg ctgcgtaatc gtgccgccgg
    19561 aaacttcttt ccattcccac actttttcca agaatttttc acgacccaag tcatgacggg
    19621 acacgttttg cgcggcaagc tgacgctcga ccacgatttg cgtggcgatg cccgcgtggt
    19681 cggtgccggg aatccaggcg gtgttgcagc ctttcatgcg gtagtagcgg gtcagaccgt
    19741 ccataatggt ttggttgaag gtatggccca tgtgcagcgt gccggttacg ttgggcggcg
    19801 gcagttggat ggaaaaagac ggtttggtca aatccatatc gggctggaaa taaccttggc
    19861 tttcccagtt ttgatagtgt ttggattcga tttcggcggg ggagtatttg tctaacatga
    19921 tggaactttg tgaaattaag gttatttttg atgtgcggat tataacgcaa aaaggccgtc
    19981 tgaatcattt cagacggcct ttggcatacg ggttttaaaa atggaacaat accaggctga
    20041 cggcaatcac cgccataccc gttgtcaggc cgtaaacggt ttcatggccg tctgaatagc
    20101 gtttggcggc cggcagcagc tcgtccaacg ccaaaaacac catcacaccg gctatcacgc
    20161 cgaataccga accaaacacg gcaggcgaca aaaacggctg caaaaccaaa tagcccaaag
    20221 ccgcccccaa cggctcggcc aagccggata gcagacacgc ccacaccgtt ttcttacggc
    20281 tgcgggtggc aaaataaacc ggcgcggcga tggaaatgcc ctccggaata ttatggatgg
    20341 caatcgccaa ggccaaaggc atcccgaccg ccggattttc caatgtggca aaaaacgtcg
    20401 ccaagccttc ggggaaattg tgcgcagtaa tcgcaaacgc cgccatcatg ccgactcgcg
    20461 cgatatggcg gcgtttgctt tcttgaaacg acgggtcttg cgcgtctaaa gtttcatgcg
    20521 ggttcggcac cagacggtca atcagcgcaa tgccgcccat cccggccaaa aatgccatgg
    20581 tcgccgccgc aaacgcgtgg tctttatcat aaatttcagc gaacgcctcg ctggacttac
    20641 tgaaaatctc cgtcagggaa acatacacca ttgcaccgcc ggcaaatgcc aaaccaaacg
    20701 acaacacacg cggattgggc gttttggaaa acattaccaa gccgctgcct aatacggtaa
    20761 acaaaccggc agccaacgtg atggaaaagg caacggccaa attggacatc gaaaaatcgg
    20821 gcatgagaaa acctgcgcta aaagctggga caggttcaga ctaacacttt ttaatgcgta
    20881 tgataatagt tattatttat tttattggtt ggatacacgg attttgaaac aaaaggccgt
    20941 ctgaaaaata gttttcagac ggcctttaca tttgaaatgc cactaaacct tatcgctttc
    21001 cagcttaagc ctgatagcgc gacaggctca aatcgtcgct gcggatttcg gtgtctttgc
    21061 cgctcacgat atcggcggtt aattttgccg aacccagcga catggtccag cctaaagtac
    21121 cgtggccggt attcagaaac aggttgtcaa agtgggtgcg accgattaac ggcgtgctgt
    21181 cgggcgtcat cggtctgagg ccgctccaga acgatgcttg gctcaaatcg ccgccttccg
    21241 ggaacaagtc gttgacgacc aaagccaagg tttcgcggcg tttttcgggc agtttgattt
    21301 cgtagcccga caattctgcc atgccgccga cgcggattct gttgtcaaag cgcgtgatag
    21361 cgactttgta gctttcatcc aaaacggtgg acaccggcgc gccgtctgaa ttggtgaccg
    21421 gcagggtcaa ggaatagcct ttgacgggat aaatcggcag atcgaggtct aactgcgcca
    21481 acacagtcct actgaagcag ccgagcgcac agacaacggc atctgcttca aaccgccctg
    21541 ttttggtttc aacggctttg atgcgcagcc cgttatggtc gatgcggctg atggtttggt
    21601 tgaaatgaaa ctgtacgccc ttttcctgac acaatttgta taggttttcg gtgaagaggt
    21661 ggcagtcgcc ggtcgcatct gcaggcaggt gcaggccgcc ggcaattttg gcggtaacgc
    21721 gtgccagcgc aggctcaaat tctgcacatt cttcgggttt cagacggcgg tacggcacgc
    21781 cgtagcgttc caaaacggca atgtcttgtt ctgccgcttc aacttcttta gcctgacgga
    21841 aaatctgcaa cgtccctttt ttgcgttcct caaaattcat gccggtttgc gcttcaaaac
    21901 ggcggaacat ttcacggcta tattcggaaa tcctgaccat gcgctcttta ttggtttgat
    21961 agtgcgctgc cgtgcagttt tgcagcattt gccacagcca ttcgatttga tacaggctgc
    22021 cgtcggggcg aaacagcaaa ggcggatggc ttttaaacag ccatttcagt gctttggtcg
    22081 gaataccggg tgcagcccaa ggcgtggtat agccgtaaga aagctggcct gcattggcaa
    22141 aactggtttc catcgccaca ccctcggcgc ggtcgatgac cgttacttca tgtcctgcct
    22201 ctgccagata ccacgcggaa gacacgccgg caacacccgc acctaaaaca agcactttca
    22261 tgtttctccc tccggctttt tcaaaacaga cttaatatgc cgtgccgtct gaatattcgg
    22321 attcagacgg cctcggatat taatgcggca attcgccatt tgtgattttt tgtttgaagt
    22381 cgcgcgtttc attgacgatg actttcgcca tcaataaaag tgcaatcagg ttgggcaatg
    22441 ccatcaagcc gttgaatgtg tccgaagcca gccacaccaa atcaaggctc aacacggtac
    22501 ccagcataac ggaagaaaca taaccgacgc ggtacaaacc ggcaaatttc tcgccgaaaa
    22561 catacaccgc gcatttttcg ccgtaatagc accagccgag aatggttgag taggcaaaga
    22621 aaatcaggcc gatggtaaca atccagccgc cgatgccggg cagcattttt tggaatgtga
    22681 cggttgtcag tgccgcgccg ctcacttcag gtttgacaaa ctcgccgccc gcgccgagca
    22741 gtcccattac caacacgatg ccggtaatcg agcaaacgac gatggtatcc aaaaacgtac
    22801 cggtcataga aaccaaggcc tgacggacgg gatggtcggt tttcgcggct gcggcggcaa
    22861 taggcgcaga acccataccc gcctcattgg agaacacgcc gcgcgccacg ccgtagcgga
    22921 tgaccgtacc gatagcgccg cccgccactg cctgcgcgct gaacgcatcg gagaaaatca
    22981 gcttgacggc aggcatcagt gcatcggaat taatcgcgat aatggaaaga ccgcccaaca
    23041 cataaaacac cgccatagca ggcacgatga aagaagcggc tttggcgatg cctttaatac
    23101 cacctaaaac gacaacggca gtcagaacgg tcaacgtaat gccggtatag gcaggttcga
    23161 taccgaagct ggtttgcacc gcctgtgcaa ccgagttgga ctgcaccgag ctgccgatac
    23221 cgaaggaagc gaatgtgccg aacagcgcaa atgcaacggc catccatttc cagtttttgc
    23281 ccaagccttt ttcgatgtaa tacatcgggc cgccggacat ttcgcctttg gaattgttga
    23341 cgcggtattt caccgccaac acgccctcgc cgtatttggt ggccataccg aaaatggcgg
    23401 ttatccacat ccaaaatacc gcgcccgggc cgccggttac caccgcagtc gccacgccgg
    23461 cgatgttgcc cgtaccgatg gtggcggaca gcgcggtcat caacgccgca aaatgggaaa
    23521 tatcgccttc gtggccttcg ccgccttcat acttctttga cggcacaaac gcctgtttca
    23581 gcgcatagcc caacatagtg aactgcaagc ctttcagcaa aacggtcagc acaatgcccg
    23641 tgccgaccag cagcatcaac ataacagatc cccagaccca gccgctgacg gtttcgaaaa
    23701 aggctttgag attgtctaaa aacaattgca tggctttctc ctttgtctgt tttattttta
    23761 aaacaccact tttgtagtgt cctataattt cagcacagaa tattcaataa gacaatatgt
    23821 tcttttgaaa aatacttttg gttttttcgc cgaaagcagg tcggttcaag ctgcggaaat
    23881 tgtttgcaat tctttaaaag cagcggcgga ggtcacaatg aaatgtccga atggggatgt
    23941 ggcgggcggc agaaatcatc aatgctgccg actgccatac ttctgaaatc tacaaaatga
    24001 tgcatcgatc aaacaatata ccgctttaaa aaaaccgatg ccgtctgaaa cgctttcggg
    24061 gcttcagacg gcatcaaaag gatacggtca gcggatgatg ccgcgcgtcg attgtgcgaa
    24121 aaagtctcgg aatacggcaa gctcggcttg ggtttcggcg cggcggagga tgtctgcctt
    24181 ggcctcttca aacggaatgc cgcgatggta gagggttttg tacacgtttt tgacggcgga
    24241 aatctgctct gcggtaaaac cgttgcggcg catgccttcg ctgttgagcc ccgccggttc
    24301 ggcgcggtag cccgatgcca taaagtaggg cggcacgtct ttgtgtacgc ctgcggcaaa
    24361 cgcggtcatg gcgtagtcgc cgatgcggca gaattggaaa accagcgtgt agccgcccaa
    24421 aacgacgtag tcgccgacgg taacgtgtcc ggcaagcgag gcgttgttgg cgaagatggt
    24481 gtggttgccg accacgcagt cgtgcgcgag gtggcagtac gccataatcc agttgtcgtc
    24541 gccgatacgg gtttcgccga tgccggttac cgtgcctaaa ttaaaggtgg tgaattcgcg
    24601 gatggtgtta ccgttgccga taatcagctt ggtcggctcg tcgcggtatt ttttgtcctg
    24661 cggaatttcg ccgaggctgg caaattggaa aatgcggttg ttttcgccga tgctggtgtg
    24721 gccgttgatg acggcgtgcg gaccgatttc ggtattcgca ccgatttgga cgttggggcc
    24781 gataacggtg tacgcgccga ctttgacgcc ggagtcgagt tcggctttgg ggtcgatgac
    24841 ggcggtcggg tggatgaggg tcatgttttg aatactccgc tgcttgttaa gacaaataat
    24901 agaaaattac agtttgtcga tttcatcgca gaccgcaatc acttgatcgc taaaatggct
    24961 aaatactgaa gcagtatcta catccatgta attaagcagt tcaaaaatag attggtgaat
    25021 atttctagcg tagttggtat cttttattgc acctttaaaa caaatcggct cgtgatgtgc
    25081 caaacgatta cggaatttgt taatattgga aagttctcga aaaatccatt tttggttata
    25141 ttgcacactt ggtgtagatt tgggcttttt tggaaaaact ttcatcaata ctttccctgt
    25201 aacatcaaat tgggcatctt tccctcctgc aaacagatac cgccaaaaac cgaatcctag
    25261 tcctgcaacc aattggttgt ggtcaaactt tcccccgcta ttattttgta ttttagataa
    25321 tgcaaccttg attaattcag cagattcttt cgtgccattt cttaaaaagc aaccttgata
    25381 ttttaatgcg ggattcgtct gtggctgtat gctgttatat aaccagtttc tgtctttaaa
    25441 cgcctgctga aggcaaatgt caattttgtt tcttaaaaca atctcaaata ttgaaacaac
    25501 atgaaaaagt tctcttgata aagataaatt caataaatac aattcagctg ctttgtcttc
    25561 cccaacggca tttttatacc gttccattct gaaatcagaa aaaatactac agcattcctt
    25621 ataatccacg ctaacacctg attctttatt tttaaattga cgtttcaaac tatatttgat
    25681 acagttaggt aaaactaatc cccaccgtgt ccctgggttt gaataaacct aaaccggtgg
    25741 gttttctttt tattaggtcg tctgaaactt tcagacgacc tttttctgaa cactcaaacc
    25801 acgcgtttgg cacacatgat gatggcttcg acggcgactt gcccgtccac tttggcaacg
    25861 gcgttgaatt tgccgatgcc gcgccggctg gtcagcagct cgacttcaaa gacgagttgg
    25921 tcgccgggga tgacttggcg tttgaagcgg gcttcgtcta tgccggcgaa gaagaagaat
    25981 tcgttttctt tgcgcccgcc ctcgctcaaa atcgccaacg tgccgcacgc ctgcgccatc
    26041 gcttcgatga tgagtacgcc gggcatcacg ggcaggtcgg gaaagtggcc ttggaactgc
    26101 ggttcgttta tggttacgtt tttgatggcg gtcagggttt tcatcggctc gaaggcggtg
    26161 atgcggtcga gctggagaaa cggatagcgg tgggggatga gtttttggat gtctttggct
    26221 tcgatgggga gttgtacgtc catgttcgtc gtattccttg aataaagtcg gtttggttat
    26281 ttgctgtctt gaccggcatc tgaaagctgc tgctccagtg ttttgaggcg tttgttcatt
    26341 tcgcttaagc ggtggatgta aacggcgttg cgcgcccatt ctttatgggt ggacatcggg
    26401 aagatgccgg cgaggtgttt gccgctttcg gtaatgctgt gggtgacgga cgtgccgccg
    26461 ccgatggtgg ttttgtcggc gatttcgatg tgtccgaccg taccgacccc gccgccgatg
    26521 atgcagtagc tgcctatggt tacgctgccg gagatgccgg ttttggcggc gatgacggtg
    26581 tgcgaaccga ttttgcagtt gtgcccgatt tggacttggt tgtcgatttt ggtgccgttg
    26641 ccgacggtgg tgtcgctcat cgcgccgcgg tcgatgttgg tgttcgagcc gatttctacg
    26701 tcgtcgccca gcgttaccgc gccggtttgc gggattttga accacgaatc gccggcaaag
    26761 gcgagtccga aaccgtccgc gccgatgacc gcgccgctgt ggatttcgac gcgtctgccg
    26821 agtgtgcagc cgtaataaac gacggcgttg ggatgcagga cgacttcgtc gcccagtctg
    26881 caatcgtgtt ggacgacggc gtttgccaag atgcggcagc cttcgccgag tacggtgttc
    26941 gcgccgatgt aggcgttcgc accgatttcg cagcttgcgg ggacggtcgc gcccggttcg
    27001 acgacggcgg tcggatggat gccgccgcgc gctttgacga cgggtgaaaa caggcgcgcg
    27061 actttggcga aatagagata ggggtcgtcg gcgacaatca ggttgcgccc ttcaaatccg
    27121 tctgccgctt tggcggaaac gatgaccgcg cccgcgctgc tgtcgtggac ttcggctttg
    27181 tatttcggat tggcaaggaa gctgatgtgt tccgcctgcg cgtctgcgag cgggcgcacg
    27241 gcggtaacgg aaatgtcctc gccgcgccat tcgccgccga gccgcgcggt gatttgggac
    27301 agggtgtagg tggccggaat catggttttc ctgttcggta tgccgtctga aagggtcagc
    27361 gggcgttcat ttctttaatg acgctgtcgg taacgtcgta ttgggtgttg acgtaaatca
    27421 cgttctgcaa aatgacatcg taaccttcct gtttggcgat tttgacgatg acgcggttgg
    27481 cgttttgctg gagggaggca aactcttcgt tgcggcggag gttgtagtct tcttcaaact
    27541 gcgcctgttt tttgcggaac gctgcgacca gcccgcgcca tttttcttcg gcttgcacct
    27601 tttttgcgtt tctgagtttg ccttcggcaa gctgcctttc caaatccaga ccttcgcgtt
    27661 gcagtttttg caattcgtcc tgacgagcgg aaaattcgct gtccagcgtt ttttgaatct
    27721 tgcgcgcctg cttggattcg aggtagatgc gctcggtgtt gataaagccg attttttgga
    27781 aggtgtcggc gtgcgcgcct gcggtgcagc acaaaccgat cagagccgcg gcaaacgcgc
    27841 gggtcaaacg ggtcatggta aaactccttc gaatgttgcc gcgaaatgcc gtctgaaggg
    27901 cttcagacgg catttgcggg attagaacgt cgtgccgagt tggaattgga agcgttggat
    27961 ttcgtcttcc ggttttttct tcagcgggta ggcgtagctg aatttcatcg ggcctaaagg
    28021 cgagagccag gtaaccgcgc cgccggcgga atagcgcaat tcgttggtaa aggtggattt
    28081 atgggtattg ccggcgccgt aaatgttttg aaccctgccg ccggtcgcgg aactgctgtt
    28141 gtcgtcgtag gttttgccgt cccacacgct gcctgcgtcg gcaaacaggc tcaggcggac
    28201 ggtgcgcgcg tctttcgcgc cgggcatcgg gaagagcagc tcggcggaga cgttggcttt
    28261 tttgttgccg ccgtagctga ttttttcgcc gtattcgtca tagactttcg gaccaagtgt
    28321 gccgctttcg taacctcgta ccgaacccag gccgccgccg tagaagtttt caaagaaggg
    28381 gatttctttg gttttgccgt agccgttggc atagccgatt tcgccgccaa gcatcagtgt
    28441 gaaggttttg ctcaggggga agaaccaggt ttggttgtgg gtggcggagt agtattgcag
    28501 tttgctgccg ggcagggcga tttcggcgtt cacgcccgtc aggtagccgc gcgtcggcca
    28561 taacgcgctg tcggttttgt tgcgccccca gccgacggta cctttgtaca gccagccttt
    28621 gaagctgccg tctgtgccgt cggttttgcc gtattgtttg ataaagtcgg catagtgttt
    28681 gggcgctttg ttgtaggtgt tgacggtcag gtgttctgcc accaaaccga aattcacgcg
    28741 gtcgtattcg gtaacaggca cgctcatgcg gatgcctgcg cctgccgtgg tggttttata
    28801 ttgtttgatg ctggtcgatg ctttgcgcgg gtcgaaggct tttccgtaaa catcgtagcc
    28861 taggctgacc ccgtctgccg tgaagtacgg gtcggtaaac gacagcgagc cgttgagcgt
    28921 ggttttgctt cgtgaggcgc gcagggcggc cgacttgccc gtaccgaaca ggttgtcttg
    28981 ggaaacgcct gcggacatga ccaggccggt atcctgtacc cagcccgcgc tcaagtcgag
    29041 cgagccggtg gaacgttcgg tcaggctcat gttcaaatcg actttgtcgg gtgtgccggc
    29101 aagcgggacg gcatcaaact gtacgttgtc gaagtagccc aaaagctcga cgcgctcttt
    29161 ggagcgttgc agcttggagg tgtcgtaagg cgcggattcc atttggcgca attcgcggcg
    29221 cacgacttcg tcgcgggttt tgttgttgcc ggtgatgtgg atttcgttga cgtagatttt
    29281 ccggcccggt tcgatgtgca ggacgaaatc gacggttttg gtttcggcgt ttggcagcgg
    29341 ctgtacgctg atttcgctgt atgcgtagcc tgccgagccc atgcggttct gaatctcacc
    29401 caaaacggcg gtcatctgct ggcgttcgta ccatttgccg ggcttcatgg tcagcagttt
    29461 ttccagttcg gctttgggga cttcgttggt gtcgccttcg atggagactt tgccccaacg
    29521 gaaacgtccg ccttcgtgga cggtgatttt gatggtctgc ttggttttgt cttcgttggt
    29581 ttggatgtcg gtatcgagga tgcggaaatc gaagtagccg ttgttctggt agaagtcggt
    29641 tactttttcc atgtcttggg caaatttctg ctcgttgaat tggttgcttc gtgtcagcca
    29701 tgtccaaatg ccgccttcgg tcagcgacat ctgccgcatc agtttgcggt cggaatagac
    29761 ttggttgcct tcaaattcga tgtcggtgat tttggcggat ttgccctcgt caatcgtgat
    29821 gtcgatgtcg acgcggttga gggcgagttt ggttactttg ggcgtgattt ggatattgag
    29881 tttgccgcgc ccgaggtatt cttctttcag gccggcgact gcctgattga gtgtcgcctg
    29941 attaaagtat tgcgactgcg ccagcccgaa cgattcgagg tttttcttaa tggcgtcgtt
    30001 ttgcagcatt tttgcgccgg tgatgttgag cgagccgatg gtggggcgtt cgataacggt
    30061 cagcaggagc tgcccgtccg cagtttcgac gcgtacgtcg tcaaagaaac cggtggcgta
    30121 caggcttttg atgatggcac tgccgtgtgt gtcgttgtag gtgtcgccga ctttgacggg
    30181 caggtagttg aatacggtac tcggctcggt acgctgcaag ccttcgacgc ggatgtcttg
    30241 gatggtgaag tcggcaagtg ccaaaggcga tatgcccaag accatcagtg cggaagcaat
    30301 ctgtttcagt ttcattgtca gttccttgtg gtgcggaatg cggtttcaga cggcattccg
    30361 aaacgtaaaa tctaaccgag cagccgggta acgtcgttga agaaggcgac cgccatcatc
    30421 agcatcatga gggcaagccc gaagcgcaaa ccgatgtttt ggacgcgttc gcccaaaggt
    30481 ttgccgcgta tccattcggc agtataaaac acgaggtggc cgccgtccaa aacggggacg
    30541 ggcagcaggt tcagcacgcc gaggctgatg ctgaccagtg ccaaaaattc caaataactt
    30601 tgcaagccga gttcggcgga ctgtccggca atgtcggcaa tggtcagcgg cccggaaata
    30661 tggctgacgg aggcgttgcc gctgattagt ttgccgaaaa atttgagggt tgtccacgag
    30721 tgggaaacgg ttttttccca gcccatgccg aatgcgcgga taacagacgg acggtagctg
    30781 cggcggattt gcgcgtccca cgccctgtcc ggctgcggac ggaggccgac gcgcccgatc
    30841 agggtgtggt cgggctgttc gacagtatcg gggcggatgt cggcggtatg ggtttgtccg
    30901 gcgcgttcgt aggtcagggt gatttttttg ccggggcttt ggcgggtcag gtttgcccat
    30961 tcctgccatg aggcgatggg tttgccgtcg gcggcagtca gcctgtcgcc cggtttcagg
    31021 cctgcttttt cggcggggct gcctttttcc acgccgccgg caacggttgt gattttaaag
    31081 ggcatcagtc cgatgtagcc ttggtttttt gcgattttac cggcttccgg cgtgcctgcg
    31141 gcatcgatgg tgcggacggt ttgcgcgccc gatgccgtct gaacgccgac ggcgactttg
    31201 ccggcttcga ggttgaggac gatttcggtt tgcgcgctgc cccaatctgc aacgggtgtg
    31261 ccgttgacgg attgtatttt gtcgccgctt tggaagccgg cgcgggcggc aatggtgtcg
    31321 ggttcgactg tgccgacata ggggcgcagt tcggttacgc cgaaggaaaa gctcagtccg
    31381 tacagcaaaa ccgccagtgc gagattggtc agcgggccgg cggcgacgat ggcgatgcgt
    31441 ttggcggggt gttgtttgtc aaaagcgtag ggtaaatcgg cttctgatac ttcgccttcg
    31501 cgcgtatcga ccattttgac gtaaccgccc aacggaatcg gggcgaggca ccattcggtg
    31561 tcgccgcgct ttcgggtgaa aaacggtttg ccgaagccga cggaaaagcg tacgactttg
    31621 acgccgcaca atctggcaac gatgtagtgt ccgaactcgt gcaggctgac caaaatcagg
    31681 atggcgaaga taaaagctag aagggtgtgc aaatggtttt cctttgataa cggtgttcag
    31741 acggcatcag cgcagtgtgc cgataaatgc ccgcgcttgt gcgcgtgtcc gggcatcttg
    31801 cgccaagagc ccccctatgt cgcctatgcc gtctgaaaag tcttgtgaaa gacaatgggc
    31861 gacggttttg gcaatgtcgg taaacttaat ctgtccgtcc aaaaaggcgg cgacggcggc
    31921 ttcgttggcg gcgttcaata cgcagggcgc ggctccgcct gcgttcatgg cttcataggc
    31981 gagcttcagg caggggaagc ggtcaaagtc gggcttttgg aaggtcagcg cggacaatgc
    32041 gtcgaaatcc aggtcgccga cacccgaatc gatgcgctcg ggcaaaccca aacaataagc
    32101 aatgggcgta cgcatatcgg gattgcccag ttgcgccaac acggagccgt cgcggtagcg
    32161 caccatgctg tgtatcacgg attgcggatg gatgacgact tcgagcttgt caggcggaca
    32221 gttgaacagc caatgcgctt caatcagctc caaacctttg ttcatcatgg tggcggaatc
    32281 gacggagatt ttgcgcccca tgctccaatt ggggtgtttg accgcttggg cgggcgtaat
    32341 gctgtcgaac gtgtttaaat cggcggtcag aaacgggccg ccggaagcgg tcaggataat
    32401 cgaagcgatg ccgtgttcgt tcagacggcc ggcgtaatcg cgcggcaaaa cttggaaaac
    32461 ggcgttgtgt tcgctgtcga cgggcagcac tgccgcgccg tttgcacggg cggtttccat
    32521 aaacaacgcg ccggaaacca ccagcgtctc tttgttcgcc agataaatgg ttttgccttt
    32581 ttgcgccgct gcgagcgcgg aaggcagccc cactgccccg acgatggcgc acatgacacc
    32641 gctgacttcg tcggcagacg caacgtcaac caatgcctgc gcgccgtgta aaacctgcgt
    32701 cgccgtgccg tcgcgtttca acagggcttc aagccgggcg gcgtgttccg catcggcaac
    32761 gacggcatat tcggggcgga acgtttgaca ttgagccgcc aatttctcga cctgcttatg
    32821 ccctgccagc gcgaatacgc ggaatttttc ggggtggcgg gagacaacgt ccagcgtgct
    32881 ttcgcctatg ctgccggtac tgcctaatat ggtcaggact tgtggtgtca taatgaggat
    32941 aactttatac cggatgccgt ctgaagcgtt ttcagacggc atagaatcaa tttaaaaccg
    33001 acatcatcgc cgcatagacg ctgataacgg caatcaggct gtcggtacgg tcgaacacgc
    33061 cgccgtgtcc ggacagcagg ttgctgctgt ctttaatgcc cgccgcgcgc ttgagccagc
    33121 tttccaacag gtcgccgcat acgctgacaa cggtcagcac caaaccgatt aacacggtat
    33181 cgaaccagcc tgtatcgaat gccagccagc cggcacttcg tacggcggtc atgtacactg
    33241 ccacgcaaac cgcgccgccg attgcacctt cccagctttt gccggggctg attgccggtg
    33301 cgattttgtg tttgccgaac gccttgccgc tgaaatacgc gcaaatatcg gcaacccaca
    33361 ccaaacccat cacggcgagc agcggcaggg catcatcggg atgcgggcgc agtgatacga
    33421 gcgcgaacca aaacggcatg accagaagcc agccgacggc ataaacctgc caaccgccgt
    33481 tgagcctcca tttgaatctc aaccataaag gcataacggc gagccaaaat gccaaaacaa
    33541 cataccaaac caaattaggc agcatccagc cgcccgcata ggcaaccacg ccgaaaacca
    33601 aggttgcggc gaggtaatgg ttggttttaa ttttgcacaa accgcccata cgggcatatt
    33661 cccacaaggc aatcagggca atcagtccgc aaaatgcagc ccacaaccat tgcggcgcgt
    33721 aaaacagcat gcccagcatc agcggcagca gccacatggc ggttattacc cgttgtttca
    33781 gcatattcag ttcctttgct gttcgatagg cagttgctcg gaggtgcgtc cgaaccgccg
    33841 ttcgcgtttt tggaacgaag cgacggcatc gtccaaagcc ttgccgtcaa aatcgggcca
    33901 caaaatatcg gtgaaataca gttctgcata tgccatctgc cagagcagga aattgctgat
    33961 gcgcgtttcg ccgccggtgc ggatgaacaa atccggttcc ggtgcatcgc ccagcatcaa
    34021 gtgtttcgcc agcgtgtctt ctgtaatctc ggatacgcct tcggcaatca gtttgtttgc
    34081 cgcctgcaaa atatcccagc ggccgccgta atcggcggca atgctcaggg tcaggccggt
    34141 attgtttgcc gtcaacgcct ccgcttcttc gatgccttgc agaatctgcc ggttgaagcg
    34201 ttcgcggctg cccagtattt tcaggcgcat attgttttcg tgcagacggc gtacctgttt
    34261 ttgcaaagcc tgtaaaaaca gccccatcag gaacgaaact tcgtcttcgg ggcggcgcca
    34321 gttttcggtt gaaaaggcaa acacggtcag atattgcaca cccagtttgg cgcaatgctt
    34381 caccatattt tccaatgcgt ccaaaccgcg tttgtgtccc attatgcgcg ggaggaaacg
    34441 ttttttcgcc caacggccgt tgccgtccat aatcacggcg atatgcttgg gaatggcggt
    34501 gtgttccaaa acggcctgcg tgctgctttt catgtctgcc tttcgcggtt cggcattcaa
    34561 atgccgtctg aacgccgaac cgtgcaggtt aaattgccat caaatcttct tctttggcag
    34621 tcaggagttt gtcggcttcg gtaatgtatt tgtcggtcag tttttgaacc gcttcttcgc
    34681 cgcgacgtgc ctcgtcttcg gaaatttctt tgtctttgag gagttttttg atgtggtcgt
    34741 tggcatcgcg gcgcacgttg cggatagaga cgcggccttc ttccgcttcg ccgcgtacga
    34801 ctttaatcag gtctttgcgg cgttcctcgg tcagcatggg catcggcacg cggatcaggt
    34861 cgccgacagc tgccgggttc agccccaagt ttgaatcgcg gatggctttc tcgactttgg
    34921 ccgccatatt gccctcaaac ggtttcacgc cgatggtgcg cgcgtccaga agcgttacgt
    34981 tggcaacttg gctgacgggg accatgctgc cccagtattc gacttccact tggtcgagca
    35041 ggccggtatg cgcgcggccg gtacgcactt tcgccagatt ttctttcagt acttcgaccg
    35101 aacgctgcat tttgccttcg gctgtttttt gaatatcgtt gatcatattg ttctttcggt
    35161 gggataaggt gggcgggaga ccgtctgaac gcgtttcaaa cctttcagac ggcatgaaaa
    35221 ctgttaactg cgaatagtac cgttattcgg gcatgacgac aaggcaggcg gattggggat
    35281 gccgtctgaa gcggcaggcg tttcagacgg catcgtgtcc gaccgtcagc cgtgttcccg
    35341 tgtttcaagc aggctttggc gcaggtgttg gcgttcgtgg gcatccagcc atttgcggcg
    35401 ggtgcgttgc agcaggatga cgagggcgga aatttcctga cgcatattgg tgctgagcca
    35461 gaggaagccc tgccattggt agtggaggtg ttcggcgagg gcttccagtt cggggttgat
    35521 ggcggtgtcg atgcggatgc ggcgggcgtg tctgccgttg ataagggcga cggtttgttg
    35581 caggtcggtt tggagcagtg tgaagtggcg gtcaagcagc cggatttcgc tgccgttgag
    35641 tttgggagat tgcagcttgg cggcagtggt caggagcagc tcggtggtgt tgacaatttt
    35701 acggtgggcg tgctgcatgg cttccatcat ggcggggctg atgcggcttt cgcccgatgt
    35761 ggcggcgagg tggctgcggc ttttgaccat gcgtgcgttg atttggcgca ttttaaccat
    35821 attctgctcc aaacgttcgc gcgtcatacg cctgccgttg ctgatttcgg caatcatttt
    35881 gctgcagtcg gtcaggttgt cggcaagcat gaaacgccac atcagtgtgg atttcagcgg
    35941 cagcagtttg gcggcggcga tggcgatggc cgcgccgatg aggacgttca tcgcgcgcat
    36001 caggccgctg tcgaaccatt cgctgccgtt gtcgccgatg agcatgcaca tcgtcagccc
    36061 cgccagcata gggacgtagc cgtttttgcc gaccgccgcc cagccggcca gtgcgcttgc
    36121 cgtgccgacg gtgaggtaga agaggaggtt gccgtggaaa taatgctggt tcagccataa
    36181 aacgcccaaa cccgcgccca gcccgatgac cgtgccgagc atacgttcca ccgccttgga
    36241 gtaaatcgcc ccttgaaact ggagcatgcc gaggacgacg aagacggtca tgcctatcca
    36301 ttcgccgtgt tggaggtgga gcagccgggc gagtgcggtg gcgaacagga cggccccgcc
    36361 gagccgcacg gcgtgtatga ggcggcggtg gcggtagcgt tcgtaggagt tgagccagcg
    36421 gctgacgagg cggttgcgtt gcgaggtgtt catatcggtt gccgtctgaa gcggaaatgt
    36481 gaaaaagcac aggcttcccg aggaagggag ggtctgtgct tggtattggt gccggagaag
    36541 ggaatcgaac ccccgacctt cgcgttacga atgcgctgct ctaccgactg agctacaccg
    36601 gcgttttttc gttatgatat atatgaacgg ttgtttgtgc aacttttctg gcgggcggca
    36661 aggcagtgcg cggtataatg cggtctgctt cggaagaggg ggacggcgat gtttgtgaac
    36721 gagaaagatc cttatgcggc tctgtttgcg gggctggtgt ttttgacgtt gccgtttgcg
    36781 ttggcggtgc atgatgcctt tgcgcttgcg ttcggacggg cagggctgct ggtgtcggtg
    36841 tcggccggcg tggcggttgg gacggtactg tttggtttgt gttcggtgtg tttgcgtttt
    36901 tgaacctggt cgtgtcggca ggcctgacga agctggcgta caaaaagatg atgcggcggc
    36961 attcccgtta cgcgctgttt ctgtcgggcg tggcggcttg cgcggcggca gcggtggctt
    37021 ggattttcga gctgctgctt ggcagtgggg ctttgggcgg gctgcgtggg gaggcggtgt
    37081 tggaatatgc gtttgccgtg tggctggtgg cgatgctgac gctgcccaaa cgcctgacgc
    37141 gcgcgccggt gcagccggtg gtgtttcaca ggaaaaaata ggttggaacg ggaaatgccg
    37201 tctgaacccc gacacgcggt ttcagacggc atgtttttcc gttaacatta cgcctgaata
    37261 tggacaggaa gcagatatgg aacgcaaaga acgcctgcaa tcaggcattg ccgcgatggg
    37321 gctggatatt tcggaaacgg cgcaggacag gcttttggcc tatgtggatt tgttgaaaaa
    37381 gtggaacaaa acctacaatc tgaccgccct gcgcgacgag gaaaaaatga ttgtccatca
    37441 tcttttggac agcctgacgc tgctgcccca tatcgagggt gtgcaaacga tgctggatgt
    37501 cggttcgggc ggcggtcagc ccggcattcc ggcggcggtg tgccgtccgg atgtgcagat
    37561 aacccttttg gatgcgaata cgaagaaaac ggctttttta cagcaggcgg ttatcgagtt
    37621 ggggttggac aatgtgcgcg tggtatccgg acgcgtggag gcggtttcgg acgtgcgtgc
    37681 cgacgtggtt accagccgtg cgtttgcaga actggcggat tttgtgtcgt ggacggtgca
    37741 tctgttgaaa gacggcggct actgggcggc gatgaagggc gtgtatccgc agggggaaat
    37801 cgaccacctg ccgcaggatg tgtgcgtcga aaaagtccaa aggcttgacg tgccgggctt
    37861 ggatgcggaa cgccatatcg tcatactccg caagcgttga gcgcgcttca gacggcatga
    37921 ataccttttt tgtgcggata aaggtaaaat tccgcactgt ttttcttttt tcaacatcag
    37981 acgggacacg ggcgggacat gagtgcgaac atccttgcca tcgccaatca gaagggcggt
    38041 gtgggcaaaa cgacgacgac ggtaaatttg gcggcttcgc tggcatcgcg cggcaaacgc
    38101 gtgctggtgg tcgatttgga tccgcagggc aatgcgacga cgggcagcgg catcgacaag
    38161 gcgagtttgc agtccggcgt ttatcaggtc ttattgggcg atgcggacgt gcagtcggcg
    38221 gcggtacgca gcaaagaggg cggatacgct gtgttgggtg cgaaccgcgc gctggccggc
    38281 gcggaaatcg agttggtaca ggaaatcgcc cgggaagtgc gtttgaaaaa cgcgctcaag
    38341 gcagtggcgg aagattacga ctttatcctg atcgactgcc cgccttcgct gacgctgttg
    38401 acgcttaacg ggctggtggc agccggcggc gtgattgtgc cgatgttgtg cgaatattac
    38461 gcgctggaag ggatttccga tttgattgcg accgtgcgca aaattcgtca ggcggtcaat
    38521 cccgatttgg acatcacggg catcgtgcgc acgatgtacg acagccgcag caggctggtt
    38581 gccgaagtca gcgaacagtt gcgcagccat ttcggggatt tgctgtttga aaccgtcatc
    38641 ccgcgcaata tccgccttgc ggaagcgccg agccacggta tgccggtgat ggcttatgat
    38701 gcgcaggcaa agggtaccaa ggcgtatctt gccttggcgg acgagctggc ggcgagggtg
    38761 tcggggaaat aggtcaatcc aaatcgggct gcccgtgcct ttatgctgtt tggccgggtg
    38821 cgttaatatg gcggattaaa ataaaaatac ttatatcgtc atttatagtg gattaacaaa
    38881 aaccagtacg gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
    38941 accaagtgaa tcggttccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcttgat
    39001 ttttgttaat ccactatatc gtcattcccg caaaaacaaa aaaatcaaaa acacaaaact
    39061 gaaatatcgt cattcccgtg caggcgggaa tctaggtctg tcggtacgga aacttatcgg
    39121 gaaaaacggt ttttccaacc ctgagactcc ggattcctgt tttcgcggga atccggtttt
    39181 ttgagtttca gtcatttttg ataaattctt gcagctttga gtttctagat tcccgctttt
    39241 gcgggaatgg cggtttggaa gttacctgaa a
//
LOCUS       DAXIHS010000007        37228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXIHS010000007 DAXIHS010000000
VERSION     DAXIHS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.007"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..808
                     /locus_tag="ACFOF2_000287"
     CDS             <1..808
                     /locus_tag="ACFOF2_000287"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7106671.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIA
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="ACFOF2_000288"
     CDS             849..1379
                     /locus_tag="ACFOF2_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC7106672.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1413..1529)
                     /locus_tag="ACFOF2_000289"
     CDS             complement(1413..1529)
                     /locus_tag="ACFOF2_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC7106673.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1754..3349)
                     /locus_tag="ACFOF2_000290"
     CDS             complement(1754..3349)
                     /locus_tag="ACFOF2_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC7106674.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWTPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3456..3851)
                     /gene="hisI"
                     /locus_tag="ACFOF2_000291"
     CDS             complement(3456..3851)
                     /gene="hisI"
                     /locus_tag="ACFOF2_000291"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC7106675.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3882..4649)
                     /gene="hisF"
                     /locus_tag="ACFOF2_000292"
     CDS             complement(3882..4649)
                     /gene="hisF"
                     /locus_tag="ACFOF2_000292"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC7106676.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4662..5399)
                     /gene="hisA"
                     /locus_tag="ACFOF2_000293"
     CDS             complement(4662..5399)
                     /gene="hisA"
                     /locus_tag="ACFOF2_000293"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC7106677.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5432..6070)
                     /gene="hisH"
                     /locus_tag="ACFOF2_000294"
     CDS             complement(5432..6070)
                     /gene="hisH"
                     /locus_tag="ACFOF2_000294"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC7106678.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGYRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6201..7703)
                     /gene="pta"
                     /locus_tag="ACFOF2_000295"
     CDS             complement(6201..7703)
                     /gene="pta"
                     /locus_tag="ACFOF2_000295"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC7106679.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMSQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7888..8946)
                     /gene="fbpC"
                     /locus_tag="ACFOF2_000296"
     CDS             complement(7888..8946)
                     /gene="fbpC"
                     /locus_tag="ACFOF2_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC7106680.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8967..10484)
                     /gene="fbpB"
                     /locus_tag="ACFOF2_000297"
     CDS             complement(8967..10484)
                     /gene="fbpB"
                     /locus_tag="ACFOF2_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC7106681.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIYWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10552..11547)
                     /gene="fbpA"
                     /locus_tag="ACFOF2_000298"
     CDS             complement(10552..11547)
                     /gene="fbpA"
                     /locus_tag="ACFOF2_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC7106682.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11799..12269)
                     /locus_tag="ACFOF2_000299"
     CDS             complement(11799..12269)
                     /locus_tag="ACFOF2_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC7106683.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESLDGREQELVLIW
                     RAEADIGNGGILQFVCNWGFPATEKTCSVLKKIGAVHSAMLIHRAADTLDKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLLNSLDEQYWQDPDKLYLLGWQYYSGNPVQTAF"
     gene            complement(12327..13703)
                     /gene="argH"
                     /locus_tag="ACFOF2_000300"
     CDS             complement(12327..13703)
                     /gene="argH"
                     /locus_tag="ACFOF2_000300"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC7106684.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQNLQTALVDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVSELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13722..14591)
                     /gene="galU"
                     /locus_tag="ACFOF2_000301"
     CDS             complement(13722..14591)
                     /gene="galU"
                     /locus_tag="ACFOF2_000301"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC7106685.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14619..15218)
                     /gene="rdgB"
                     /locus_tag="ACFOF2_000302"
     CDS             complement(14619..15218)
                     /gene="rdgB"
                     /locus_tag="ACFOF2_000302"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC7106686.1"
                     /translation="MSEKTEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYSTFVENALAKARHATKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHQDDPRPIIAEGVWHGQWHGTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15211..15444)
                     /locus_tag="ACFOF2_000303"
     CDS             complement(15211..15444)
                     /locus_tag="ACFOF2_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC7106687.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15552..16085)
                     /locus_tag="ACFOF2_000304"
     CDS             complement(15552..16085)
                     /locus_tag="ACFOF2_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC7106688.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPTIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESVERWNKQA"
     gene            complement(16187..16645)
                     /gene="nudB"
                     /locus_tag="ACFOF2_000305"
     CDS             complement(16187..16645)
                     /gene="nudB"
                     /locus_tag="ACFOF2_000305"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC7106689.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16856..16932
                     /locus_tag="ACFOF2_000306"
     tRNA            16856..16932
                     /locus_tag="ACFOF2_000306"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16890..16892,aa:Pro,seq:ggg)
     gene            17245..19137
                     /gene="mafB"
                     /locus_tag="ACFOF2_000307"
     CDS             17245..19137
                     /gene="mafB"
                     /locus_tag="ACFOF2_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC7106690.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISESSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGEIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEMKKGRGG
                     KPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFTEPNRTAY
                     PLYNSK"
     gene            19164..19583
                     /gene="mafI"
                     /locus_tag="ACFOF2_000308"
     CDS             19164..19583
                     /gene="mafI"
                     /locus_tag="ACFOF2_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC7106691.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            19673..19744
                     /locus_tag="ACFOF2_000309"
                     /pseudo
     CDS             19673..19744
                     /locus_tag="ACFOF2_000309"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            19741..19983
                     /gene="imm31"
                     /locus_tag="ACFOF2_000310"
     CDS             19741..19983
                     /gene="imm31"
                     /locus_tag="ACFOF2_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC7106692.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            20061..20306
                     /locus_tag="ACFOF2_000311"
     CDS             20061..20306
                     /locus_tag="ACFOF2_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC7106693.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            20309..20698
                     /locus_tag="ACFOF2_000312"
     CDS             20309..20698
                     /locus_tag="ACFOF2_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106694.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSCSLSKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            20746..20916
                     /locus_tag="ACFOF2_000313"
                     /pseudo
     CDS             20746..20916
                     /locus_tag="ACFOF2_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            20919..21317
                     /locus_tag="ACFOF2_000314"
     CDS             20919..21317
                     /locus_tag="ACFOF2_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106695.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            21418..21807
                     /locus_tag="ACFOF2_000315"
     CDS             21418..21807
                     /locus_tag="ACFOF2_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106696.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            21955..22344
                     /locus_tag="ACFOF2_000316"
     CDS             21955..22344
                     /locus_tag="ACFOF2_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106697.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(22385..23842)
                     /locus_tag="ACFOF2_000317"
     CDS             complement(22385..23842)
                     /locus_tag="ACFOF2_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC7106698.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(23857..24687)
                     /locus_tag="ACFOF2_000318"
     CDS             complement(23857..24687)
                     /locus_tag="ACFOF2_000318"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC7106699.1"
                     /translation="MAHYAIGDIQGCFDELTSLLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(24765..25154)
                     /locus_tag="ACFOF2_000319"
     CDS             complement(24765..25154)
                     /locus_tag="ACFOF2_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC7106700.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(25290..25814)
                     /gene="nspA"
                     /locus_tag="ACFOF2_000320"
     CDS             complement(25290..25814)
                     /gene="nspA"
                     /locus_tag="ACFOF2_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC7106701.1"
                     /translation="MKKALAALIALALPAAALAEGASSFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGMRVKF"
     gene            complement(25985..26179)
                     /locus_tag="ACFOF2_000321"
     CDS             complement(25985..26179)
                     /locus_tag="ACFOF2_000321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106702.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            complement(26399..27574)
                     /gene="hemW"
                     /locus_tag="ACFOF2_000322"
     CDS             complement(26399..27574)
                     /gene="hemW"
                     /locus_tag="ACFOF2_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC7106703.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCFL"
     gene            complement(27631..30156)
                     /gene="ligA"
                     /locus_tag="ACFOF2_000323"
     CDS             complement(27631..30156)
                     /gene="ligA"
                     /locus_tag="ACFOF2_000323"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC7106704.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(30304..31590)
                     /locus_tag="ACFOF2_000324"
     CDS             complement(30304..31590)
                     /locus_tag="ACFOF2_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC7106705.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(31784..32356)
                     /gene="ampD"
                     /locus_tag="ACFOF2_000325"
     CDS             complement(31784..32356)
                     /gene="ampD"
                     /locus_tag="ACFOF2_000325"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC7106706.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            32440..33435
                     /gene="mltG"
                     /locus_tag="ACFOF2_000326"
     CDS             32440..33435
                     /gene="mltG"
                     /locus_tag="ACFOF2_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC7106707.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            33495..34115
                     /gene="tmk"
                     /locus_tag="ACFOF2_000327"
     CDS             33495..34115
                     /gene="tmk"
                     /locus_tag="ACFOF2_000327"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC7106708.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            34337..35617
                     /locus_tag="ACFOF2_000328"
     CDS             34337..35617
                     /locus_tag="ACFOF2_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC7106709.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            35853..36056
                     /locus_tag="ACFOF2_000329"
     CDS             35853..36056
                     /locus_tag="ACFOF2_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106710.1"
                     /translation="MKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDSFGQF
                     LFGCSAEATRTAPISVKRSCLFT"
     gene            36164..37198
                     /gene="lpxK"
                     /locus_tag="ACFOF2_000330"
     CDS             36164..37198
                     /gene="lpxK"
                     /locus_tag="ACFOF2_000330"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC7106711.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
BASE COUNT         8341 a         8689 c        11040 g         9158 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcagatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaacta ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aacgcggaaa tggaatttga tagggatata tcaagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagcgtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcaggat
      781 tttaaaagca atcaggggga atatttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttctg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaagggt
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gtatcggcaa ccgcgtcctg gtcggtatgg gcagcaccgt tttggacgat gccgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgtgcctcc gcgcaaacgt ttggcgggcg
     1261 gctaccttta tgtcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg atgggacgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaaagccttt tttcgggttc agacggcatt ttatcgccga ttgcttttta cagtttgacc
     1561 gaatgttcgc gcgtttcgtg gaacacgatg tccggccaac gttcttgcgt cagccctaaa
     1621 ttcatgcgga cgggatgccc gataacggta tccgatgcgc cttgattttg atcggtgcat
     1681 ttgagttgca ggcaaaaatg ccgtctgaag cccttttttc gggttcagac ggcattttat
     1741 tgccgattgc tttttaaagt ttgaccgaat gctcgcgcgt ttcgtggaac acgatgtccg
     1801 gccagcgttc ttgcgtgagt cccagattca cgcggttagg ggcgaggtag gcgaggttgc
     1861 cgcccgcgtc gatggcgagg ttgcctgcat tggctttttc aaattccgcc agttttttct
     1921 tgtcgtcgca cgatacccag cgcgccgacc agatggatgc gctgtcgaac acggcttcta
     1981 cgccgtattc gttggccagc cgcgaggtaa cgacttcaaa ttgcaacacg ccgaccgcgc
     2041 ccaaaatcaa atccgcgccg ctcatcggtt tgaacacctg caccgcacct tcttcgccga
     2101 gttgttgcaa gcctttttgc agttgcttga ttttcagcgg gtttttgatg cggacgctgc
     2161 ggaacagttc gggcgcgaag aatgggatgc cggtgaacgc cagttgttcg ccttcggaga
     2221 agctgtcgcc gatttggatg ttgccgtggt tcgggatgcc gatgatgtcg ccggcgtagg
     2281 cttcttccac cagctcgcgg tcgtgcgaca tgaaggttac cacgctggag gcggcgattt
     2341 cgcggttgat gcgaaggtgt ttcatcttca taccgcgctc gaatctgccg gagcagacgc
     2401 gcaggaaggc gatgcggtcg cggtgtttcg gatccatatt ggcttggatt ttaaagataa
     2461 atccggagaa tttcggctcg tccggctcga ccatacgtac ggtcgcgtcg cgcggtttcg
     2521 gcgcgggcgt ccagtcaatc aatgaattga ggatttcctg aataccgaag ttgttaatcg
     2581 cagagccgaa gaatacgggc gtgagttcgc cggcgaggaa ttcgtcgaga ttaaactcgt
     2641 tggaagccgc ctgcaccaat tcgatttcgt cgcgcaactg ctggatttcc aatggaaagc
     2701 gttgttccaa ttcgggattg tcgatgcctt tgatgatgtc gaactcgtgc ggcagacgtt
     2761 cgccaccggc ttcaaagaga tagatttcgt cgttcaggat gtggtacacg cctttgaagt
     2821 ttttgcccat gccgatcggc caggttacgg gcgcgcagcg gatttgcagg atgttttcca
     2881 cttcgtccag caactccaaa gaatcgcgca cttcgcggtc gtatttgttc atgaacgtga
     2941 caatcggcgt attgcgcagg cggcagacgt tcaagagttt gatggtttgc gcttccacac
     3001 cttttgccgc gtcgatgacc atcaacgcgc tgtccacggc ggtcagaacg cggtaggtgt
     3061 cttcggagaa gtcttggtgt cccggcgtgt ccaagaggtt gacggtgtgg tctttgtagt
     3121 cgaactgcat cacgcttgat gccacggaaa tgccgcgctg cttctcgatg tccatccagt
     3181 cggaggtggc gaatttgccg gtttttttgc cttttaccgt acccgcgctc tgaatcgcgc
     3241 ccgaaaacag caacagcttt tcggtcagcg tggttttacc cgcgtcgggg tgggagatga
     3301 tggcaaacgt gcggcggcgg cgcacttggt cgaggatttc ttgggacatg gttttctttg
     3361 caaaaaggtt caggccgctt ttcagacggc ccggacagtg tttgagacgg cgaaattgta
     3421 caaaaaaatg cctgataatt caatgttgga ggcggtcagt gcgtgctgcc gtaaatctct
     3481 ttttcgtctt tcaggacggc atcggcggtt tcccacgcgc cgccgcgcca gactttgtaa
     3541 aagcagcttt ctcgcccggt gtggcaggcg atgccgccgt tttgggcgat gagcatcaca
     3601 atggcgtcgc cgtcgcagtc gaggcgcagt tcgcggactt tttgcgtgtg tcccgactct
     3661 tcgcccttca tccattgttt ttggcgcgaa cggctgtaat agtgggcaaa gccggtttcg
     3721 acggtttttt gcagggcttc ggcgttcatc cacgctacca ttaaaacacg tttggtttcg
     3781 gcatcttggg cgatggcgca aaccaaacct ttttcgtcaa atttgacggc ttcaagcagg
     3841 tttttatcca tatttccttt cagacggcat ggtcggggcg gtcagaggcg cacttcgatg
     3901 ccggcttcgc gcatagcgcg tttggcttcg cggatggcga tttccccgaa atggaaaacg
     3961 ccggcggcaa gtacggcatc ggctttgcct tcggttatgc cttcaatcag gtgccggaca
     4021 ttgccgaccc ctccggaggc gatgacgggg atgtcgacgg cttcggcaac ggcgcgggtc
     4081 agcggcaggt tgaaaccctg tttcgtaccg tccctgtcca tcccggtgag caggatttcg
     4141 cccgcgccgc gtttttgcat ttcgatcgcc cattccaccg catccaaacc ggtcggattg
     4201 cgcccgccgt gggtaaagat ttcccagcgt gtgttttcgg agttgacggc tttggcatcg
     4261 acggcggcga cgatggcttg cgaaccgaaa aatccggcgg cttcgtcaat caggtcggga
     4321 cgggtaacgg cggcggtgtt gatgctgact ttgtccgcgc cggcattgag caggcggcgg
     4381 atgtcggcaa cggtgcgtac gccgccgccg acggtcaggg ggatgaagac ttgtccggca
     4441 acctcttcga tgatgtgcag gatggtgtcg cggttgtcgg atgaggcggt gatgtcgagg
     4501 aaggtcaatt cgtccgcgcc ttcgccgttg tagcgtttgg cggcttcgac ggggtcgccc
     4561 gcgtcgcgca aaccgatgaa gttcacgcct ttgacgacgc gcccgtcttt tacgtcgaga
     4621 caggggatga tgcgttttgc cagtgccata atcggatgcc tttagtcgag ggaatctgcc
     4681 agttgctgcg cttgggcaaa atcgatgctg ccctcgtaaa tcgcgcggcc ggtaatcgcg
     4741 cctgctacgc catgtttttc ggcggcacac agggcgcgga tgtcgtccaa gccggtcagt
     4801 ccgccggagg cgatgacggg aatgcggacg gcttgggcga gtttgaccgt cgcgtcgatg
     4861 ttcacgccgc tcatcatacc gtcgcgcccg atgtcggtgt agatgatgct gttgacgccg
     4921 tcgtcttcaa agcgttttgc caaatcaatt acatgatgcc cggttacggt tgcccagccg
     4981 tcgatggcgg ccataccgtc tttggcatcc agcccgacaa taatcctgcc ggggaaggct
     5041 ttgcacgcct cgcgcacgaa gtcggggttt ttgaccgccg ccgtgccgat aatcacgtcg
     5101 tttaagccca aatccaaata ttgtccgatg gttttcaaat cgcgtatgcc gccgccgagc
     5161 tgtacgggga tgtctttggc gacagcggca aggatgtctt tgatggcggg caggttttgc
     5221 ggaacgccgg caaacgcgcc gttcaaatct accagatgca ggcggcgcgc gccttgtttg
     5281 aaccagtgca gcgcggtttc ggcgggcgaa tcggaaaaga cggtcgcctc ttccatcagc
     5341 ccttgtttca ggcggacgca gcgtccttct ttcaaatcga tggcgggtat cagcagcata
     5401 atttttctcc ttgtgcgggg ccgtgtccgg cttaccagtt taaaaagttt ttcaacatcg
     5461 tcagcccggc atcgtggctt ttttcggtgt gaaattgcgt ggcgaatacg ttgtctttgc
     5521 cgacgatgca ggcaaacggg gacgggtagt cgctttcgcc caatatggtt tcgggatttt
     5581 cgggggcgaa atagtagctg tggacgaagt aaaaacgcgt gtcttgggga atatctttaa
     5641 acagcgggtg gttttgggtt tggcgcacgg tgttccagcc catatgcggg actttcagac
     5701 ggtatccctg cgggtcgcgg aggtcgcgct caaagcgtct gactttgccg ccgaaccagc
     5761 ccaagccgtc ggtgtttcct tcttcactgt ggtcgaataa aagttgcgcg ccgacgcaga
     5821 ttccgaaaaa gggtttgttt tttaaggcat ctttgactgc ctcgtccagg ccgccgcgcg
     5881 tcagtgccgc catacagtcg ggcatcgcgc cctgcccggg aaaaatgact ttgtcagcgc
     5941 gggacacgcg ttcggggtcg ccgcttaaaa agatttcggt attttttccg gcaagctgcc
     6001 ccgccgtccg gacggatttc aatacggaat gcaggttgcc cataccgtaa tcgataatgg
     6061 cgatttgcat ggcttcctcc tctttttttg caatatggct gcgattttaa caaacaaatg
     6121 tgccgtgctg ataaaaatgc cgtctgaaaa cgggagtctg tcttcagacg gcatagggtt
     6181 caaacccgga aagccgtttg tcagccttcc atttgttttg cctgaacggc agtcagggcg
     6241 atggtgaaca cgatgtcttc caccagcgcg ccgcgcgaca ggtcgttgac cggtttgcgc
     6301 aggccttgca gcagcgggcc gacgcttaag acgttggcac tgcgttggac ggctttatag
     6361 gtgcagttgc cggtgttcag gtcggggaag accaaaacgg ttgcctgtcc tgccaccggg
     6421 ctgcccggag ctttggattt gcccacgccc ggcacggttg ccgcatcata ttgcagcggg
     6481 ccgtcgatgg cgaggtcggg gcgtttttcc cgggcaagtt tggttgcttc gatgacgata
     6541 tcgacatcgg ggccgctgcc ggagttgacg gtggagtagg aaatcatcgc cactttcggg
     6601 tcgatgccga aggcttttgc ggaatcggca gactggatgg cgatgtcggc aagctgttgc
     6661 gcggtcgggt tcggattaac cgcgcagtcg ccgaagacga ggacttggtt gggcagcagc
     6721 ataaagaata cgctggacac gaggcttgcg cccggtgcgg ttttaatcag ttgcaaagcg
     6781 gggcggatgg tgttggcggt ggtgtgaacc gcaccggata ccaaaccgtc cacatcattt
     6841 tgcgacatca tcatcgtacc gagtaccacg gtgtcttgca gttgcttgcg cgcgtcttcg
     6901 ggtgtcaggc ctttggattt gcgcagttcg cacatcggct cgacgtattg ttcgaccaat
     6961 gaggcgggat cgatgatttc caaagagtcg ggcaggctga tgccgcgttc tttggcaacg
     7021 gcttcgactt cttcgcgttt ggcaagcagg acgcagcggg caatgccttt ttcgtggcag
     7081 atggcggcgg cttggacggt gcggggttct gcgccctcag gcaggacgat gcgtttgtcg
     7141 gcttggcggg cgaagtcgat caggttgtag cggaattgcg ccggcgacag gcgttttgct
     7201 tcgcggcctg ccaatacgga tacgtctttc agcgcgtcgc tcgaaccgaa gaaggtcagg
     7261 ccggtttttt cggctgccgc ttcggcaacg gaggctgccg cgccgtccac gacaaaacct
     7321 tccaatacgc ccggcgcggc ggcgaagaac tgtttggcaa ggttcagccg atttgccagt
     7381 tcgtcggcat cggcgttgtc ggaacggacg gcgaagacgg ctgccgcgtc aagggacaat
     7441 gccagttcga cgtttttgcc tgcgaggtag attttgtcgg catcgggcgc gatgccttcg
     7501 atgacgaggt tggcggcatc gagtgcggca actttgccga ccagtgcgtc gaaccagtcg
     7561 tcgcttttgc cttgcgcgag cagggtttcg gcggttgcgt caacggcttg gaaaatttgt
     7621 gcgtccagtg cttttgcaaa ggcttgtgcg gcggcggagg cgtccagtcc ggcagatacg
     7681 ggtacgatga gtacttttgc catgatatat cctttcgtat gctgcggtgt gcggcatatg
     7741 tggttggaag gggcggcata taggcagaaa cggctgcctg cgtgccgtgc gtgccgtgtt
     7801 tggcttgagg cgcgcaggtt gaatatagca aacaaattct gtttccaaca agataaatat
     7861 ccgcaggctt gtggatgctg ccgcctttca gagggtattt ccggggaaga acagggcggg
     7921 accgtccaaa tggaggacgg cggaaatgcc gtctgacagg gtgggggcgg aagggaggtt
     7981 gagcgtgagg acggtttgtc cgacccggag actgatttcg gtatgccgcg ctttgggcgt
     8041 ggttttgaga accacggcgt gaatggaggc ggtgggtgcg gaatgggggt gaaggctgaa
     8101 ctgttccgga cggatgagca gtgtgccgcg tgtgcctgcg ggtgcgccgc tttgaacggg
     8161 caggcggccc aatccgcaat cggcggtgcc gtcggcgttg agcgcggcgg ggaacacgat
     8221 gccttcgccg ataaacaggg cggcatcaag gtcggcaggt tgtcggtaca attcgtgagg
     8281 gcttgcagtt tggaggatgc gcccctgttt catcacggca atccggtcgg cgtattgcag
     8341 ggcttcttcg cggtcgtggc tgacgaaaac ggcagatttg ccgttggcgc gcagggcggc
     8401 aatcatgtct tcgcgaatct ggcggcgcaa ctgttcgtcc agcgcgctga agggttcgtc
     8461 caacaaaatc agttcgggat cgggtgcgag ggcgcgggcg agggcgacgc gctgttgctg
     8521 tccgcccgaa agttcgtgcg gatagcgtcc ggcaagttcg gaaatgccgg tcaattccaa
     8581 catagcttcg atgcgctgcc gctcttgcgc cgtcttgcct ttgccgttgc ccagcccgta
     8641 ggcggtgttg cggtaaacgg tcaggtgggg gaacagcaca ccttcctgta cgacataacc
     8701 caaacggcgt tcgcggacgg ggaggttggt atttttcgag aagatggttc tgccggaaag
     8761 cgaaatttcg ccaaaatcgg gttgttcaaa accggcaagg cagcgtaaaa gggtggtttt
     8821 gccgcagccg gacgcgccga cgataaagag gatttcgccc gggtcgaggc tgagcgaaat
     8881 gtcgtttaaa actggggtgt tttgaaaact tttggacagg tgtccgatgt gcagggcggc
     8941 ggtcatggcg gtacttcctc aagctgttat ttgaaggcgt atttcttcag caggaatacg
     9001 gggatgccgg aaaataatac cagcatcagc gcgtaagggg tggcggcggc gtattgtgcg
     9061 tccgatgtgt attcccaaac ggcggtggag agtgtgtgga catcgtcggt ggtcagcagc
     9121 agggtggcgg tcagctcttt catcagtttg aggaagacga gtgcgaatgc ggcggtaatg
     9181 ccgggcagga tggacggcag taccaacgtc ctgaaaataa agaagtgtcc gcgccccaat
     9241 gttgcgccga cctgttccat cccttttggg agttgttcca aggaagtcct cagggtggtt
     9301 tgcgccatcg gcaggtaaag catgaaatag gcaaggatga cgacgataaa ggtttggtaa
     9361 acggcagggg tgtagttgat gctgaaataa accaaggata gggcgataac caaaccgggg
     9421 acggcgtgca gtaaaaacgg cagcctgtct atccaaacgg ttaaaaaatt gcgatagcga
     9481 accgatgccc aaacaagggg caaggcacat aatatagtca aaatcgcacc taaagccgat
     9541 acgcttaagg aacggataaa ggcatcaaat acggatacga gcgcgaatgt gccggaagtg
     9601 ccgaccatca tccaatatat caatacgcca aaggggataa taatgcccaa agtcaacaag
     9661 ctgcttaaaa aaacaatcgc gccaatctga ccgggcagtt tgagggtttt gacgggataa
     9721 ggacgggcaa cgcctttgcc gctgtggtaa atcttggctt tgccgcgaaa tatgctttct
     9781 ccaaatacga cgatgccgca caccgccatt aaaacagcgg aaagcagggc ggcggtattg
     9841 ttgttgtagg acatttcgta ttcttggaaa atggcggtgg taaaagtggg gtagttcaaa
     9901 atggataccg cgccaaattc gaccagcata tgcagggcaa tcagtaacac gctgctgccg
     9961 atggcgggtt tgagctgggg gaggatggcg gaaaaaaagg tttgcaggcg gcttttgccc
    10021 aaggacaggc tgacttcttc gtaagacagg ctgatgcgtt tgagtgccgc ctcgacgggc
    10081 aggtaggcga gcgggaacga ggacaggctc ataatcatca ctgtccccca aaagccttcg
    10141 acacggaagg tcaggctgat ccaggtgaaa cagctgacaa atgcggggat gcacaaaggc
    10201 agggtgattg ccgtctgaaa aaaggttttg ccgaagaagc ggtaacgttg gaacaaaagg
    10261 gcgcaggcaa tgcccaaaac aatggaaatc agggtaacgc ccgccatcat cgtcaaggtg
    10321 ttggagagca aatcccacat acgcgggcgg aacaacagtt cgacggcgcg gttgatgccg
    10381 acctgccacg aacgcatagc gacatataaa aaaggcaggg taagcggtag ggcaatcagt
    10441 aggatgaggc cggtaagcca aatgggtatt tttttaggag acatagtgtt tttcatcggc
    10501 aaaacgggcg gacagtataa atgtccaccc gtttgacaat ccgaaaacgg cttatttcat
    10561 accggcttgc tcaagcagcc gggtggcgtg ttctttttcg gaaacagtgg tggcggacac
    10621 ttggggtgct tccaacttgg cgatgggttc caaattgaag gtggatacca cgtgcggatt
    10681 caaaggatat tcggcacgga cggcggtcag ggcgcgctgt ccttccttgc tggcgaggaa
    10741 ggcgacgaat tttttcgcct catccttgtt ttgggaggat tttaacacgg ctgcgccgga
    10801 ataggtaatg agtgcgccgg gatctctgtg gcggacgaaa ttcaggcggg tgtggacatt
    10861 ttgtacgcct ttttcacgcg cgaaagcgtg ccagtagtag ttgttgatga gggcggcatc
    10921 gatttcgccg ttttcaaccg cttgaagggc gacggagttt ttagcgtaag gcttgccgta
    10981 ttctttcaga cctttgagcc atttcaatgc ggccgcttcg cctttcagtt tgacgatggc
    11041 gacaacctgt tccaagaacg cgccggaagt gggggcgtaa ccgatgcggt ttttccattt
    11101 cggcgtggcg taattcagga cggatttttc caaatctttt tcagacagtt tgcgggtgtc
    11161 gtaaacgacg acgcgcgaac gtccgctcag tgccacccag tcttttttgg cggcaaccgg
    11221 cacacccttg ccgcgtgttt cgttgatggt ggaggcgggc aggggctcta ggaggttggc
    11281 tgcggaaagg gtggcgagtg ccgggatttg ttcggaatag aatacgtcgg cggggcttcg
    11341 gctgccttct tctttgattt gaccggcaag ctggtcgcct ttggcgctgt tgagtttgac
    11401 tttgatgccg gtagcccggg taaaggcatc tgcaacggct tgtgccgctt ctttgtgttg
    11461 gccgttgtac acggtaatgt ctgccagcgc gggggttgcg gcggtcaggg ctgcggcaag
    11521 cagtgcgtat cggatagatg ttttcatatc gattttctcc taatgaatga gggtgtatat
    11581 cttgttaaga cataacgggg tgtagtgtat tccttctttt ttataaatgc aaataattat
    11641 ttttttaaat ttgttgttgt ccgatccggt tatggtttgt tatgacttgt attttttccg
    11701 tgcatcgcgc ccgtaaggcg gaagcggcga gtaatgcgtg tcggaatgtg ggtaaaggcg
    11761 gcattttgat ttgtcggaat gcttgagaac atctcccttt aaaacgccgt ctgaacaggg
    11821 ttgccggaat agtattgcca tcccagcaga tacagtttgt cggggtcttg ccaatattgt
    11881 tcatccagac tgttcagcag tgaggcggtt ttgttgtcga gatgttttgt tatatcccac
    11941 atttctttca ggtttttacc ttccgattgg aggtggcgga tttctttgtc caatgtgtct
    12001 gccgcccgat ggatcagcat tgcgctgtgt accgcgccga tttttttcag aacggaacaa
    12061 gtcttttcgg tggcgggaaa accccagttg cagacaaatt gcagtatgcc gccgttgccg
    12121 atgtcggctt ccgcccgcca aatcaaaacc agttcctgtt cccgtccgtc caagctttcg
    12181 agtttgccgt catgctgttc aaaaagtttg tccaccgcct gccacatcat ctgttcgaag
    12241 cggtcggctt gggtatccgt atcggtcatc gtgttcttgc cttttaaaaa tgccgtctga
    12301 acatttcttc agacggcatt tgggggttaa gccaacattt cccgccagcg tttcacttgg
    12361 aagcggactt gttccggcgc ggtaccgccc aagtggttgc gggcgttgag gctgccttcg
    12421 ggtgtcagta cgccgtacac gtcgtctgaa atcagcttgc tgaagccttg tagaacttcg
    12481 agcggcaatt cgctcaaatc gacgccggct tcatcggcgt gtcgcacggc ttgggcgacg
    12541 acttcgtggg catcgcggaa gggcatacct tttttgacca gataatccgc caagtcggtg
    12601 gcggtggcaa aaccctgcat cacggcggcg cgcatattgt cgggtttgac ggttacgccg
    12661 cgcatcatat cggcgtaaat ccgcagtgtg tcgatgagtg tgtcggcagt gtcaaacagc
    12721 ggttctttgt cttcctgatt gtctttgttg tacgccaagg gctgggattt catcagggta
    12781 atcaggccga taaggtgccc gataacgcgt ccggatttgc cgcgcacgag ttcggacacg
    12841 tcgggatttt tcttctgcgg catgatggac gaacctgtgc agaaacggtc ggcgatgtcg
    12901 ataaagccga aacgcggact catccacaaa atcaattctt cagacaggcg gctcaagtga
    12961 accataacca gcgaggcggc ggcggtgaac tcgacggcaa aatcgcggtc ggatacggca
    13021 tcgagcgagt tttggcagat ttgctcgaaa cccagtaatt cggcggtaat ttcgcgccga
    13081 atcgggtagg tcgtaccggc aagggcggcg gcaccgagcg gcatacggtt gacgcgtttg
    13141 cggcagtcgg ccatgcgctc gaaatcgcgg ccgagcattt caacgtaggc gagcatgtga
    13201 tgcccgaagc tgaccggctg ggcaacttgt aagtgggtaa aaccgggcat gacggtttcg
    13261 gcattttgtt ccgccaaatc aaccaaggcc gtctgaaggt tttgaatcag gctttgaata
    13321 acggtaattt gatcgcgcag ccacaggcgg atgtcggtag cgacttggtc gttgcggctg
    13381 cggccggtgt gcaggcgttt gcccgcatcg ccgattttgt cggtcaggcg gcgttcgatg
    13441 ttcatgtgga catcttccaa atcggacgac cattcgattt tgccgctgcg gatttcttcg
    13501 aggatttccg ccatgccccg acggatgtcc gccaaatcgc cttcgtccaa cacgccggtt
    13561 tctttcagca tttgcgcgtg ggcaagcgag ccttggatgt cccattcggc aaggcgtcgg
    13621 tcgaaaccga tggaggcggt gtattgtttg acgagttcgg aaacgggttc gttgaaacgt
    13681 ccggaccagg ttttgtcgtg cataaggatt ccttgatgtg gttattcggt acggtatttt
    13741 tccaaaaggc ggcggaaggg ttcgccggtt tcggggtgtt tcaggccgta ggcgacggtg
    13801 gcttccagat agcccagttt gctgccgcag tcgtagcgcg tgccttcaaa ggggtgcgct
    13861 aggacaaatt cgtgatcgag cagcttggcg atgccgtctg taagctggat ttcgttgccc
    13921 gcgccgcgcg gcagtccggt cagtaagtcg aaaatgcgcg gggtgaggat gtagcgtcca
    13981 acaacggcaa ggttggaggg cgcgtcttcg ggcttgggtt tttcgacaat gccggtaatg
    14041 cgttggaact gtttgagctg ttcggtttcg acgatgccgt atgagccggt ttgcgatgct
    14101 tcaacggttt ctacgcccaa aatgctgttg ccgctgcgcc cgtacacttc gaccatttgt
    14161 ttgagcgcgc ctttgggggc atcaatcagg tcgtcggcaa ggataacggc aaagggttcg
    14221 tctccgatgg cggcgcgggc gcacaagacg gcgtgtccca agcccagtgc ttccgcctga
    14281 cggatgtaga ggcaggtaat gttcggcggc aggatgttgc ggacgtgttc caacaatttg
    14341 tctttatggc gcatttccaa ctcggtttcg agttcgtatg ccttgtcgaa atggtcttcg
    14401 atgctgcgtt tgttgcgtcc ggtaacaaac accatttccg tgcagccggc ttccacggct
    14461 tcttctacgg cgtattggat cagcggcttg tcgacgatgg gcagcatttc tttcgggctt
    14521 gccttggtgg cgggcaggaa gcgtgttccc atccctgcga cggggaaaac ggcttttttt
    14581 atcggtttca ttctttttcc tttgtattgt tttgatgttt aaagggcgag tttgcggatt
    14641 aaatcggcga gtgcctgcgc gcggtggctt tcgcggtttt tgacctccga atccaattcg
    14701 gcggcggttt tgccgtgttc gggcagataa aaatacgggt cgtaaccgaa accgttttgc
    14761 ccgagcggcg tgccgtgcca ttgtccgtgc cacacacctt cggcgataat cgggcgcggg
    14821 tcgtcttgat ggcggacaaa aaccaatacg cagacgtagc agcagctttt gtcggctttg
    14881 ccggcaagtt cggcggcaag tttcaggttg ttggcggtat cggatttggg attgtcgccc
    14941 gcgtaacgtg cggaatggat gcccggcgcg ccgtttaagg cggcggcaca gatgccgctg
    15001 tcgtcggcga gtgcgggcag cccgctgtat ttggtggcat ggcgtgcttt tgccagcgcg
    15061 ttttcgacaa aggtggaata gggttcgggg cattcgggta tgccgaatgc ggattgcggc
    15121 aatacggtga tgccgtaagg tttgaataag ttgccgaatt ctttgagctt gccagcattg
    15181 ccgcttgcca aaacgatttt ttccgttttt tcagacatag cggttttcct ttgcggcgga
    15241 ttgggcggcg cgtagggatt tgtgccgcag gtagagggcg agtgcgccga tttgcccgaa
    15301 cagcgcgccg aatgcgaaaa gaaaggacgc gacggcgaag gctttgctgc cggcggtcag
    15361 cagataagca cccaagagga cgagcagcat aaatgccagt gtgaagaggg caacagacaa
    15421 cagatagatt tttcggcggt tcatggcgtt cggtcggaaa cggtatgttc ggattatatc
    15481 cgattgggac ggtattccct agagcttgga aaaatgccgt ctgaagcggc gttcagacgg
    15541 catggcgggc gttatgcctg tttgttccaa cgttcgacgg attctttgat gacttttttc
    15601 gcttcttccg catcgcccca ggattcgact ttggttgtac ctgctttttt caggtctttg
    15661 tagtggttga agtggaattc gatttgtttg atgagctgtt gcggcaaatc ggacaaagtt
    15721 ttgtaggcgt tgccgttgtt gcggtcgtcg gcggggacgc agacgatttt gtcgtccact
    15781 tcgccatcgt caacgaattt catcacgccg ataacgcgcg cttccaagaa tacgccggtt
    15841 gccagaggct gttcggtaac gagcagcacg tccaattcgt cgccgtcttc gtctaatgtt
    15901 tggggaatga agccgtagtt ggtcggtttg gcgaagatgg tgggttcgac gcggtcgagt
    15961 tggaatgcgg cgagtttgcg gttccattcg attttgtggt tgctgccggc ggggatttcg
    16021 ttgacgacgt tgatgatgcc gccgtccacg tcgccggggg tcaggatttg gttgaagtct
    16081 gccatttggt ttcctttatt tgggaaagtt tgaagtttga aagtatagca caaacgtccg
    16141 gctggaaatg cgcccgatgc cgtctgaaag ggtgtacggg cgcgtgttac cgtttgccca
    16201 aaaacctgcc cagttccaga atcgcgcgcc tgttggacgg ggaaaacact ttttccgccg
    16261 cttcttccaa gccgaaccag ccgtaggaga cgtgttcctc gggttgcagg gcgatgggcg
    16321 tatcgcgcgg gatttcggca gagaagaggt gttcgcggtt ttcaaacacg ccttttggat
    16381 agcggtgccg ccagtggtgg tagatttcgt aaaccgtgct gtcgtgccag tcttgaagct
    16441 gcccatccgc cagcaggatg ccggtttctt cccaaacttc gcgccttgcc gtttgggcga
    16501 cggtttcgcc cggttcgagg ctgccggtta ccgactgcca aaatcctttc ggatgcgtgc
    16561 gttcgatgag caggatgccg ccgtccccgc tataaaggac gaccagtgcg gagacggggt
    16621 atttgagcgg ttttgccatc ggcatctttc ggcgggctgc ggtaatgaag gggcggatta
    16681 tagcaaacgc cgcacgttat ggcgtttatc cttttccgta tccttttttc tgcacggatg
    16741 ggacgcgccg gtgtttgccg gtaaattttc cgttgtgtca aaaagataag ggcggttgtg
    16801 attttaatgc ttgccaaagc gtcgggcgga aactataatc cgaaacttat cgagtcggag
    16861 tgtggcgcag tctggtagcg cacttgcatg gggtgcaagg ggtcgaaggt tcgaatcctt
    16921 tcactccgac caaaaattcc gaaagccgct ttcaaaagcg gcttttttgc cgtccgtatg
    16981 attatgatgt agagtacgcg gcgacagaca ttcaaatgcc gtccgaaaac cgttcagatg
    17041 gcatttcttt atcttaatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
    17101 cagtacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
    17161 tttgagctaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat agtttcattc
    17221 cgtaccatct taaggaacat caaattgggc atttcccgca aaatatccct tattctgtcc
    17281 atactggcag tgtgcctgcc gatgcatgca cacgcctcag atttggcaaa cgattctttt
    17341 atccggcagg ttctcgaccg tcagcatttc gaacccgacg ggaaatacca cctattcggc
    17401 agcagggggg aacttgccga gcgcagcggt catatcggat tgggaaacat acaaagccat
    17461 cagttgggca acctgatgat tcaacaggcg gccattaaag gaaatatcgg ctacattgtc
    17521 cgcttttccg atcacgggca cgaagtccat tcccccttcg acaaccatgc ctcacattcc
    17581 gattctgatg aagccggtag tcccgttgac ggatttagcc tttaccgcat ccattgggac
    17641 ggatacgaac accatcccgc tgacggctat gacgggccac agggcggcgg ctatcccgct
    17701 cccaaaggcg cgagggatat atacagctac gacataaaag gcgttgccca aaatatccgc
    17761 ctcaacctga ccgacaaccg cagcaccgga caacggcttg ccgaccgttt ccacaatgcc
    17821 ggcgctatgc tgacgcaagg agtaggcgac ggattcaaac gcgccacccg atacagcccc
    17881 gagctggaca gatcaggcaa tgccgccgaa gccttcaacg gcactgcaga tatcgtcaaa
    17941 aacatcatcg gcgcggcagg agaaattgtc ggcgcaggcg atgccgtgca gggtataagc
    18001 gaaagctcaa acattgctgt tatgcacggc ttgggtctgc tttccaccga aaacaagatg
    18061 gcgcgcatca acgatttggc agatatggcg caactcaaag actatgccgc agcagccatc
    18121 cgcgattggg cagtccaaaa ccccaatgcc gcacaaggca tagaagccgt cagcaatatc
    18181 tttacggcag tcatccccat caaagggatt ggagctgttc ggggaaaata cggcttgggc
    18241 ggcatcacgg cacatcctgt caagcggtcg cagatgggcg agatcgcatt gccgaaaggg
    18301 aaatccgccg tcagcgacaa ttttgccgat gcggcatacg ccaaataccc gtccccttac
    18361 cattcccgaa atatccgttc aaacttggag cagcgttacg gcaaagaaaa catcacctcc
    18421 tcaaccgtgc cgccgtcaaa cggaaagaat gtgaaactgg caaacaaacg ccacccgaag
    18481 accaaagtgc cgtttgacgg taaagggttt ccgaattttg agaagcacgt gaaatatgat
    18541 acgaagctcg atattcaaga attatcgggg ggcggtatac ctaaggctaa gcctgtgttt
    18601 gatgcgaaac cgagatggga ggttgatagg aagcttaata aattgacaac tcgtgagcag
    18661 gtggagaaaa atgttcagga aacgagaaga aggagtcaga gtagtcagtt taaagcccat
    18721 gcgcaacgag aatgggaaaa taaaacaggg ttagatttta atcattttat aggtggtgat
    18781 atcaataaga aaggcacagt aacaggaggg catagtctaa cccgtggtga tgtacgggtg
    18841 atacaacaaa cctcggcacc tgataaacat ggggtttatc aagcgacagt ggaaattaaa
    18901 aagcctgatg gaagttggga aatgaaaaaa ggaaggggcg gaaaacctat gacaaatcat
    18961 tctatgttcc caaaagattg ggatgaggct agaattaggg ctgaagttac ttcggcttgg
    19021 gagaaaagaa aggaattaga aggtcaaaaa tggagaggta caagtaaatc tggtgttgaa
    19081 atagaaggat ttacggaacc taatcgaaca gcttatccac tttataatag taaatgaagt
    19141 attgattaaa aaggtgtttt taaatgtata aaataaaatg tttttatgat cagtttagtt
    19201 ctggtgaatt atttaactac tgccagtttt tagataattc ttctaatcaa aagataaata
    19261 cacgaggaac ggtgtatcaa tatattattt atgttttaac aggagacctt tatctacaaa
    19321 aagacataga tgaaaattta gaatttatcc atcaagcaga aaataatccc aatgaagttt
    19381 attcaggagg tggacaaggt ttttgctggg atatttctgc tgaaaaagta gttttctatc
    19441 acaacgaatt tgatgaagaa gacggctggc ctgatttaag ctgttcgctg catactttta
    19501 aaactgcttt aattgcttgg aatgcttttt tgcaattgcc taaaagtatt cattcggtgg
    19561 tggagactgt gattgaggaa taagcataat tagcttaatg aatagaatca gcgatataga
    19621 ttggactgca aatccacgct tatacgctgt gccatgatta acaggaagag agcaacttgg
    19681 atgggctagt agggatggta agcatttaaa tatatcaatt gatggaaaga ttacacacaa
    19741 atgactaatt taaaattaga tttctactct gaagttatta taaaagattc ttgccctaat
    19801 gatttgttag aaaatggtga gactattaaa ggaaaaaaag gagtggtatt aggtataagc
    19861 gaagaagatg gtataatcta tggctatact attttacttt ttgatataaa atattgtata
    19921 tatatagata agaaatatat tataccgact ggaaaaaaat tctctcgcga tgatttttat
    19981 tagctaattt aagtaattta agcaagtttt ttagatactg aaactggtaa ttatgtttat
    20041 gtaagcataa ggagttcaga ttgaaggatt ctacagggca aatgaggctg gcaaccaagg
    20101 atttggcgga agccattaaa cgaggagaag tacgtagttc tgcttttaca acaaagcaac
    20161 taaaggcaat cgaaaaagga gaatctaaaa taccaaatta cacttggcat catcatcaag
    20221 acactggaag gatgcaattg attaatgaag acttgcatca tgataccggc catatcggtt
    20281 ggagagcgat gagtaaagga aagtaactat gtggaaaatc ataaaagagg atagtaatga
    20341 tttaggattt gcaattaaat gcttattctc tcagtctatt gatttaaatg aattcaaatt
    20401 atggattgaa caagtaatac gagatatgcc catcgaggac atcccgtttt atatttttga
    20461 tttggcggat tttgatgggg gaattgccga tattgacaat attgtaggtt ttgtttcgag
    20521 ttgcagccta tcaaaatcga aaaaaaatgc cttgacgggc attgccttct taaggggtat
    20581 agatgtctat gaccctcccg tttcaaaaga aaaagcatta aaagccttag agaaatatcc
    20641 tgaaatttat cagaagtttc agcatttctt tccgtttgta gagctgcctc cgctttaaac
    20701 agtcaaaatg ccgtctgaaa caatattcgg ctttcagacg gcatttttga tacaaagcag
    20761 gtaaccaaaa gagtgtttga cggaaaagga gaagctaaaa taccgagcta tacttggcat
    20821 catcatcagg atacgggacg aatgcagtta atatccgaaa gaaagcatac gcaaacaggg
    20881 catataggtg gtgaagcaat ggataaagga aaatgattat gttggggctt acacggaaca
    20941 attgtgatga tttgggattt gctatattgt gttatgagcg caatgcagcc gacaaaaaag
    21001 aattcttgga ttggttatat ggaattgtca ggactgtccc tgccgaaagt tttccaaggt
    21061 atctgattga tttaatagat tgtttggaat cagatgattg ttccataaat atatataata
    21121 ttattggctt tgttccaact accggtatga cgaaaaagga aaaagatgcc gtatatggca
    21181 ttacctatat acggaatcaa gagttttacg atattccgat tactagggag aaggcgttaa
    21241 aagccttaaa aaaacatcct gaaatttatc agaagtttcg gcatttcttt ccgtttgtag
    21301 aactgccccc gctttaaaca gtcaaaatgc cgtctgaaac gatattcggc tttcagacgg
    21361 tatttttgat ataaagcggg taactaaaag agcgtttgac ggcaaaggaa gataattatg
    21421 tggaaaatta taaaagagga tagtaatgat ttaggattcg caattaaatg cttattctct
    21481 caatctattg atttaaatga attcaagtta tggattgaac aagtaatacg agatatgccc
    21541 attgaggaca tcccttttta tatttttgat ttggcggatt tcgatgtagg aattggcgat
    21601 attggcaata ttgtaggttt tgctccgagt tccagcctgc cgaaatcgaa aaaaaatgcc
    21661 ttgaccggca ttgccttctt aagggggata gatgtctatg accctcccgt ttcaaaagaa
    21721 aaagcattaa aagccttaaa gaaacatcct gaaatttatc agaaatttca gcatttcttt
    21781 ccgtttgtag agctgcctcc gctttaaaca gtcaaaatgc cgtctgaaag accgtccgcc
    21841 gttcagacgg catttttgat ataaagcgga taactaaaag agcgtttgac ggcaaaggag
    21901 aatctaaaat accggatgta tcggttggga agcaatggat aaaggtaaat aattatgtgg
    21961 aaaattagta aagaaaattg tgaagattta ggatttgcaa tagtctgtat gttctatgat
    22021 gctattaatc tttctgaatt taaattatgg ttggatatag ttgtcagaga tactcctatt
    22081 gatacaattc cattgtatat ttttgatttg attgattttg ataagagtat aggggaaatt
    22141 tatgatgtaa ttggagtcgt taattatggt tacatttcaa atgatcaaaa aaatgcatta
    22201 acgggcattg ccttcttaag ggggatagat gtctatgatc cgcctatttc aaaagaaaaa
    22261 gcattaaaag ccttagagaa atatcctgaa atttatcaga ggtttcagca tttctttcct
    22321 tttgtagagc ttccgctttt ttaaaagaca atatgccgtc tgaaaagttt tcagacggca
    22381 ttttttattt cttccagtag gcgggggtga agaggatgaa gacggtgaag atttccagcc
    22441 tgcccaagag catggcggta acgcagatcc atttctgcat cacgtccaaa ccggcgtaat
    22501 tgccggcggg cccgacttcg cccaggccgg ggccggcgtt ggtgatgcag gcgatgacgg
    22561 cggtgaaggc ggtggtaaat tccataccgc tcgccatcag caggaagctg aagaggacga
    22621 cggtcataaa gtagatgaag atgaaggaca taacggtcag cgcgaggcgg tcgggtatgg
    22681 ccttgccgct gattttgacg gtgcggacgg ctttggggtg cagcagcacc atcatttcgc
    22741 gcaggctgaa tttgaacagg acgagggcgc gtatggtttt gatgccgccg ccggtcgagc
    22801 cggagttggc gaggatgttg gcgaggaaaa acatccacag ggaaatcagg agcggccatt
    22861 gtgcgaagtc ggtgttggcc agcccgtttg ccagtccgat ggagacgaag ttgaaggcgg
    22921 tgtagcgcag ggattcggta aaaccggcgt aatagccggt gtgccacagg tacagggcgg
    22981 cggcaaggat gctgccggag agcagcagca gcatcgtccg gcattcttcg tctttccaat
    23041 aggttttgag gctgcggctg ttgagggcgg cgaaatggtt ggcaaaattg atgccgccga
    23101 caatggtgaa aacgatgatg accgcttcga tgagggggga gttgtaataa gctatgctgg
    23161 catcgtgggt ggaaaacccg cccagcgaga gggtcgccat cgcgtgacag acggcatcga
    23221 accagcccat cccggcaaaa tgcaggcagg ctgccgcgag gatggtgatc agggtgtagc
    23281 cgaaccagag ttttttcgcc acttgggaaa tgcgcggcga cattttgctt tctttgtcaa
    23341 tgccggggat ttcggctttg aataattgcg tgccgcctac gccgagcata ggcaggatgg
    23401 cgacggcaag gacgatgatg cccatcccgc ccagccagtt gagcatatgc cgccaaaagt
    23461 tgacggaggg ggcgagcccg tcgacgtggg ggatgacggt cgcgccggtg gtggtcagtc
    23521 ccgacatcga ttcaaaaaat gcgtcggtaa agcccatatt cgggaaatac aggtacatcg
    23581 gcatcgcagc catagcggca aacgccagcc acaacatcag gacgagggtg aagccgtcgc
    23641 gcgggcgcag ttcgcgcctg aaccggaggg tggcgagccg gacgatgtac gagccggaaa
    23701 gggtaacggt cgcggtggtg gcgaaggcgg tgtacgcgcc gtccgaaaag gcgtaggaga
    23761 gggcggcggg tatcagcagg ataaaggaaa acagcatacc cagtcgggag aggacgtggg
    23821 cgatgggcag gattttgtgc atagtggggc ggtccgttat tttgcgaagc ttttccagtc
    23881 tatgccgccg gcggcttgga cttgggtaat ttcttccgtt tcgaggttga cggcggtcag
    23941 ctgtccgccc cacagcgcgc cggtgtccag cgagatgacg ttgtcggcat tcgtgtagcc
    24001 cagcgaggac cagtgtccga agatgatggt gcggtcgagg ttttgccggt cgggggcttt
    24061 gaaccacggg cgcaggtaag gcggcatttt tttcactgtg gatttgtagt cgaaatccag
    24121 ttcgttttta aaggtcaggg cgcgcatccg cgtgaaggcg ttgacgatga agcgcaggcg
    24181 ggcatagcct tccaaacctt cgtcccacgc ggccggtttg ttgccgtaca ttttggagaa
    24241 gaatttgacg tattttttgc cgcgcagttc ggcttcggct tctctagcga gcgattcggc
    24301 tttggcgatg cgccattgcg gcaggatgcc ggcgtgtacc atcacgcggc tgccctcgcg
    24361 tatcaacagc ggttgcgcac gcagccagtc gagcattttt tttccgtcgg ggtgtttgag
    24421 tatgggttcg attgtgtcgc tgcgtttggg cgcgccttcg ccgcagccga cagcgagcag
    24481 gtgcaggtcg tggttgccga ggacgatttg cacgctgttt tcgtgccgga tgcagaattg
    24541 cagcgtttcg agggatttcg ggccgcggtt gacgatatca cccgtcagcc agagggtgtc
    24601 cgtgccgtgg ttgaagccga ttttgccgag cagtgaggtc agttcgtcga aacagccttg
    24661 tatgtcgccg attgcgtaat gtgccattgc agatgttgtg aagtgggaaa gtgttgcggt
    24721 tcggacggca tggttttgaa atatcatgca gtccgaacgt ggaattatgc gttcaaaacg
    24781 aggacggctt cggcttcgac ctgcacgcct ttgggcagcg aggcaacgcc gacggcggcg
    24841 cgggcgggga agggctcggc gataaattcc gccatgactt cgttgaagac ggcaaaattg
    24901 cccaagtcgg tcaggtaggc gttgagtttg acgatgtcgg ccagcgtgcc gcctgccgct
    24961 tcggcgacgg cttgcaggtt ttggaacact tggcgcgctt cggcgcggaa atcgccgttg
    25021 ccgacgacgg tcatcgtggc gggatcgagg gggatttgac cgctcatgta aacggtgtcg
    25081 cctgctcgga cggcttggct gtacgcgccg atggcggcgg gggctttgtc ggtgtggatg
    25141 atggttttgg acatttcgga ttcctcaaaa aatagggcgg cagaagccgc agcattcggg
    25201 attatcgtac aaaaccgccg gcttgtgtag ttgcggtggc agaaaacaaa accgccgaag
    25261 gctcggcggt ttgcagaata aggcgcatat cagaatttga cgcgcatacc ggcggacagt
    25321 tcgccggaac ggacgttttt gacagtgttg actttgccga tgtagttgta gcggtagccg
    25381 gcatccaaat cgacattcgg ggtaacggca tagcttacgc ccgccaatac gccgaggccg
    25441 gtggaggttt ggctgaagct gtcgctgccg cccaagtcga cggaggcgcg gttgaggctc
    25501 aagcgcgcgc cgagatacgg tttgacgggc gattgggtgt cgaagtcgta aatggcggac
    25561 gcgccgatgc tgtaaagttt gaaatcggtg gatggggctt tatagttttt gtagcgcgtg
    25621 taatcgacgg cgaagcggag gtcgttgatg cggtagcctg cggagatgcg cgggctgaag
    25681 cctttggcag aacctaaaga gcttgaggct tttgcgtgtg cggcatcggc ttggacgtaa
    25741 aagctggatg cgccttccgc cagtgcggcg gccgggagag cgagggcaat cagtgcggca
    25801 agtgcttttt tcatattttg gttcctttat ggtcagttag aaaaattgtt aagaatccgt
    25861 taaagaatcc tgctgtatta tacttaaatt ttctttttgc atcgtaatat tttcaatact
    25921 tcaagatacg tagcggtatc cggctgcttt gccgacggca aagccgttaa cccgcgcgtt
    25981 gcctttaaat ggtggcggcg gcatcacgcg gcggatgggt gaaacttgca aacggtttgg
    26041 aaaaaacagc ggtatctgtc ggattgttgc aggtgcaggc atacggtttt gtgtgcgtct
    26101 gtgccttaag cgtcggacat ttccggcggc ggctgtgccg tctgaaacgc ccggcggggg
    26161 atgcggctgc gttttccatc gataagcata ttttccggac gcgttcgggg cgggttttcc
    26221 cgggcggccg ccgatttgtt tgcgcttata tagtggatta acaaaaatca ggacaaggcg
    26281 acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta
    26341 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact
    26401 ataaaaaaca ctgcagcaaa tcgtttaaaa acaagcgtcc tttttcggtc gggcggaata
    26461 cggcagggtc ggtttccagc aggccttttt gccttgccgt ttcgatttgc gccatgattt
    26521 tggcactcgg tacgcccgtg cgctcctgca acatcgcggc gggtacgccg tcggtcaggc
    26581 gcagggcgtt catcatgaat tcgaacggca aatcttcggc agcgacggtt ttgcgttcga
    26641 cggcttcact cggttggctt tgcattaagg cgaggtagtc gttggggtgg cggcggcgga
    26701 cggtgcgctc gatgcggtcg ggataggaaa ttttgccgtg cgcgcccgcg cctatgccta
    26761 aataatcgcc gaactgccag tagttcaaat tgtggcggca ctgcatggct ggtttcgcaa
    26821 aagccgatgt ttcgtagtgg acaaaacccg cgccttccag cgcgccgtgt accgcgtctt
    26881 cgatgtcgag ggcggcttcg tcttgcggca aacctttcgg cggcgtatga ccgaacggcg
    26941 tgttcggttc catcgtcaga tgatacgcgc tgatgtgggt cgcgcccgta gcgatagcgg
    27001 tttgtacgtc gtccaatgcc gtctgaacgg tttggttcgg cagggcatac atcaagtcga
    27061 tattgacttt atcaaataat ttcaaggcgg tatcgatagc ggttaaggct tccttaccgt
    27121 tgtggacgcg ccccagcctt gagagcatat cgtcgttgaa actctgtacg ccgatagaaa
    27181 gccgcgtaat acccgcgtct ttaaatcctt gaaacttctc gatttcaaat gtccccggat
    27241 tggcttccaa ggtaatttcc gcttccggct gcaagcgcaa cagcgaacgc acgccgctta
    27301 acaaacggtc aatcgattcc gcctgaaaca ggctgggcgt accgccgccg aaaaagatcg
    27361 tttccaccgg cctgccccaa atattgggca attcaagctg caaatcggtc agcagcgcgt
    27421 cgatataggc ggcttcgggc aatccgtttt tcaggctgtg ggaattgaag tcgcaatacg
    27481 ggcatttttt gatgcaccac gggatgtgga tgtaaagcga caggggcggc agggcggtga
    27541 gtcgggtgcg gtttggaaag gaaatggtgt gcatggtgtg gttcggaaaa gtgggcaatg
    27601 ccgtctgaag gcggttcaga cggcatgggt tcagccgagc agggtaagca gttcggcttc
    27661 gctgaggacg gaaacgccca aggcattggc tttttccagc ttgctgcccg cggcttctcc
    27721 ggcgacgacg taatcggttt ttttggacac gctgccggaa actttgccgc ctgcggcttc
    27781 gattagggat tgggcttggt cgcgtttgag ggtgggcagt gtgccagtta acacgaaggt
    27841 tttgcccgct acggctttat tgatgccgtc tgaaccttgc gccgcctcgt cttcagacgg
    27901 catttgcgcg aagaaggttt tcaggttttc gagtagggcg gcgttttgtg gttcgctgcg
    27961 ccatgcttgc cagtcggcgg ggagggattt gtcggtttgc agaccctcta tacttttgcc
    28021 ggcgagttcc cataaggctt gggctttgtt ttcgctaatt ttgaaaccgg gcaggcgggc
    28081 aatccagcgt tgcggttcgg catattgtgc ggcaggcagg ctgacggctt gggcttgcgg
    28141 ggcaacgcct gcggcgagca gctcgtctat catcgcctgc tgttcggctt gggcgaagaa
    28201 gtgggcaatg gaacgcgcca ctaccgtacc gatgtcgggc aggcaggcga ggacgggttc
    28261 gggggcgcgg cggacgcgtt ccaatgtgcc gaatgcctgt gccagcgttt tggcggtgcg
    28321 ttcgccgacg tggcggatgc cgagcgcgaa caggaagcgc gcgagttcgg gcgttttgcc
    28381 ggattctatg cctgcgagga tgttttccgc ccacttggtc ggttgttttt tatgtttgcc
    28441 cgacgcaccg accgaactgc cttcagacgg catttgatcc gattcggcaa cggttttgtc
    28501 cgccgtttcc ttcatttttt gcagggtcgg aatatcgagg cggtagaggt cggcgaagtg
    28561 gcggacgagg tcttgggcga caagctgttc gatttgtttt tcacccaagc cgtcgatgtc
    28621 catggctttg cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat
    28681 accgccgctg catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat
    28741 ggggcaatgg gtcggcaggc ggtagggctt gtagagcgga acggattggg tttggtttgc
    28801 agccggtgct tcggcaaaca aatcgtcctg ccgatgcccg atgccgtctg aaacagcaac
    28861 ggcggtttcc tgcatcgggc ggcgttcaaa aatcacacgc acgacttcgg gaatcacgtc
    28921 tccggcgcgg cgcacgacga cggtatcgcc gacgcgcacg tcttttcggg aaacttcgtc
    28981 ctgattgtgc agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgtaatcg
    29041 ggcaaccggc gttaccgcgc ccgtcctgcc gatttgcacg tcaatcgctt cgacgacggt
    29101 cagggcttct tcggcaggga acttgtgggc aatcgcccag cgcggcgcgc gggagatgaa
    29161 gccgagttcg cgctgttgcg ccaggctgtt gactttaacc accatgccgt cgatttcgta
    29221 gggcagttcg gggcgttttt gctgcatgtg ttcgtaaaac gccaatactt cgtcgatatt
    29281 tttgaaacag ccgaaattgc cattgggcag actgaagccg agtgcttgga aataggcgag
    29341 ttcttggatg tgttcttccg cgacgaaacc gccttgccgg cgggcgacgg agtaggggaa
    29401 aaagtgcagt ttgcgttgcg cagtgatgcg cgaatcgagt tggcgtaggc tgccggcggc
    29461 ggcgttgcgc ggattggcaa agggtttttg cccgttttcg gcttgtcttt tattgagggc
    29521 ggcaaaatcg gctttttgca tcagtacttc gccgcgtact tcgatgagtt cgggcgtatt
    29581 ttcgccgtgc agccgcaagg ggatgttgga tacggttttg atgttttggg taacgtcttc
    29641 gcccgtcgtg ccgtcgccgc gcgttgccgc ctgcaccaat acgccgtcgc ggtagagcag
    29701 gctgatggcg aggccgtcga atttgggttc gataacgtat tcgggattgc cgccgtccaa
    29761 gccgtcgcgc acgcgttggt cgaaggcgta catttcggca tggtcgaaca cgccgttttc
    29821 atcttgcggg gaaaaagcgt tggtcagcga cagcatcggc acttcgtggc gtacttcggc
    29881 aaatcccgcc aaaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gtttgagctc
    29941 gggatggttt aactccaacg cttcgagttc gcggaacaat ttgtcgtatt cggcatcggg
    30001 tacgctgggc gcgtcgaggg tgtagtattc gtaggcgtag cggttgagga ggtcggtgag
    30061 gcggcagatg tgttgtgcgg caaattgttt tatatcacta tcagacggtt taagaagatt
    30121 ggtaaagtta gtgttatgtt ttgagtttgg attcatgaga gaaggttttc agacgacctt
    30181 tgtctgatac gggatgaaac gggcaaaggt cgtctgaaaa atgataggtt gaaaacagct
    30241 gaattttacc cgaaaaaaag cggatatgcc gtaacgacat atccgctttg attgcattcg
    30301 attttaggag aacaggcgca atgcggtttt gccgcccggt tcgataccga ctttgagcat
    30361 ctcggactga cgcgccaata cataagtgcg cacgtctttg agccattggg tcgaaacttc
    30421 ttccattttg tcgttgacca gattcaggtt caactggccg gacaggcgta ccgccaaatc
    30481 cataaacaaa tcgtcgaagg ttttttcgcc tgccggagag tgcgggatgt cgagcagcat
    30541 actgaagcct ttgtaggact ggttgtccaa aagggcgttg gtaaacggct cgttgttgag
    30601 cgagcagatg gagaacatgg tcgagcccga cgtgtcggta tagtggaacg cgccgtcgtc
    30661 ttccaaaacg aaacccacgc ccgttacggc ggaacgcagt tctacgccgc tgatgctggt
    30721 cggggaaacc aaatggatgg cgatggtctg gtcgacgcgc gcgcagaatg cgtccagtgc
    30781 ggaagccact tcgataaagg cggcaaggtc ggtgtgcagc gtctgaccgc ccatgctttg
    30841 tgcgaatgcg tccacctggc ggttgaatgc ggagagttct tcctgcgagg caagtccgtt
    30901 gcggctgact gcctgaatac ccacgataaa tgcctgatag cggatgcccg ggatgggttc
    30961 ggcaatctgg aaatggtcgt ccatggtgca gccgacaatc tggtagcggc agcggttgga
    31021 aaggcgtggc agtgcgtgca gttctttggc ttcggtcagc gcgatatagg agatgaagtc
    31081 gaagcgcacg tcaaaccagg gtaattcgac ttttgacagt tctttgagcg taatcagcgg
    31141 ttttgcaggt gtttgcggaa cgggtgcagg ttttgccggc gcgtcggcag gtttcggtgc
    31201 ggaatgtccg gtttggggtt cggaaacggt gtgggcggag ttgccgataa tgccgctttc
    31261 ttccaaggcg gtttcgattt cggttttgaa cggggaggct tttgcctgtt tctgcttggc
    31321 gatgtagacg gcatcctgtt cttgcaggtt gcgcatagca gggtcttgga gttttgccgt
    31381 ttttttgacc gccggttggg gtttcggcat catgactgac ccgccggacg gtttgccgtc
    31441 gcggacatgg ctggttttgc tgttgagcag ggcatctttg tcggagtgtc cgaactggtc
    31501 gcgcactttt ttgcggtatt ggttttcctg atacatattg taggcgacaa cggcgaggac
    31561 ggcggcgagg aacagtacga tgtaaatcat ggcaatcact ttaaatttcg ggatgcagga
    31621 tgcgcgaagt gcgggtactg cggttaaatc gggcttgcgt ttccggcagt ctgatggaac
    31681 ggccggttat aacgtttgaa ttataacgaa aattgcaggg tctgacagca gtgtgtcgaa
    31741 ataagcggaa attttccgaa atgccgtcta aaatctgtgg ttttcagacg gcatttctgt
    31801 ccacgggaaa ccctttcccc cgtatccgcc gccagtcgaa aaaatgaccg gggtcggttt
    31861 tgcggccggg cgcgatgtct tggtgccccg ttaccgccgt gatggggtag cggcggcaga
    31921 ttgcgtccaa caaggcttcg agcgaacggt attgcgcttc ggtaaagggt tcgaaatcgc
    31981 agccttccag ttcgatgccg atggaaaatg cgttgcattt ttcccgtccg cgaaacgagg
    32041 atacgccggc atggtatgcc atattgtcgc aggaaacgaa ctgtaccgtt tcgccgtcgc
    32101 gtttgattaa aaaatggctg gatacgcgca aagtgtgtat caggctgaag aacggatgtc
    32161 cgtcggggtc gagccggttg gcaaacagct tttccaccgc atccgtacca tattcgaacg
    32221 gcggcagcga aatgttgtgc aacacgatca gggaaaccgt ttcccccgtt tccctcgggc
    32281 tgaaattggg cgacggggta tggcgtatgc tttgaagcca gccgttttgc cagtgtgctt
    32341 cggcgtgatt gtccatgatg ttcttcctgt ccggcgggca atttgggtta tactgtcgcc
    32401 cgaattttaa gacgtattcc gaatgctggg aatcctacca tgttgagaaa attgttgaaa
    32461 tggtctgccg tttttttgac cgtatcggca gccgttttcg ccgcgctgct ttttgtccct
    32521 aaagacaacg gcagggcata caggattaaa attgccaaaa accagggtat ttcgtcggtc
    32581 ggcaggaaac ttgccgaaga ccgcatcgtg ttcagcaggc atgttttgac ggcggcggcc
    32641 tacgttttgg gtgtgcacaa caggctgcat acggggacgt acagactgcc ttcggaagtg
    32701 tctgcttggg atatcttgca gaaaatgcgc ggcggcaggc cggattccgt taccgtgcag
    32761 attatcgaag gttcgcgttt ttcgcatatg aggaaagtca tcgacgcaac gcccgacatc
    32821 gaacacgaca ccaaaggctg gagcaatgaa aaactgatgg cggaagttgc ccctgatgcc
    32881 ttcagcggca atcctgaagg gcagtttttc cccgacagct acgaaatcga tgcgggcggc
    32941 agcgatttac ggatttacca aatcgcctac aaggcgatgc aacgccgact gaatgaggca
    33001 tgggaaagca ggcaggacgg gctgccttat aaaaaccctt atgaaatgct gattatggcg
    33061 agcctgatcg aaaaggaaac agggcatgaa gccgaccgcg accatgtcgc ttccgtcttc
    33121 gtcaaccgcc tgaaaatcgg tatgcgcctg caaaccgacc cgtccgtgat ttacggcatg
    33181 ggtgcggcat acaagggcaa aatccgtaaa gccgacctgc gccgcgacac gccgtacaac
    33241 acctacacgc gcggcggtct gccgccaacc ccgatcgcgc tgcccggcaa ggcggcactc
    33301 gatgccgccg cccatccgtc cggtgaaaaa tacctgtatt tcgtgtccaa aatggacggt
    33361 acgggcttga gccagttcag ccatgatttg accgaacaca acgccgccgt tcgcaaatat
    33421 attttgaaaa aataaaccat gccgtctgaa aagtttgtgt tttcggacgg cataccctta
    33481 ccggaactgc aagcatgaaa ccgcaattca tcactttgga cggcatagac ggtgccggca
    33541 aatccaccaa ccttgccgtc atcaaggcat ggtttgaacg gagggggctg cccgtgctgt
    33601 tcacgcgcga accgggcgga acgccggtcg gtgaggcctt gcgcgaaatc ctgctcaacc
    33661 ctgaaaccaa agccggtttg cgtgcggaaa cactgatgat gttcgccgcc agaatgcagc
    33721 atattgaaga cgtgatattg cctgcacttt cagacggcat ccatgtcgtg tccgaccgtt
    33781 ttaccgacgc aaccttcgcc tatcagggcg gcgggcgggg gatgccgtct gaagacattg
    33841 aaattttgga acattgggtg cagggcggtt tgcgccccga tttgaccctg ctgttggatg
    33901 tgccgctgga agtgtcgatg gcgcgtatcg gacagtcgcg cgagaaagac cggttcgagc
    33961 aggaacaggc agatttcttc atgcgtgtgc gcagcgttta tctgaaccgt gccgccgcct
    34021 gtcccgaacg gtacgccatt atcgacagta accgcagctt ggatgaagtc agaaacaaca
    34081 tagaaaaagt gttggacgga catttcggct gttaatgcgg caaatattga aacaagcgca
    34141 tccacccgcg ccgaaaatca aacggcagtg ccgcaggtga aaatggcggt atgcgccaaa
    34201 ctttcggcat gatagaatta cgctcggtta caaggcagga tgcgtcggca atattaacga
    34261 accgcccgta acatgatgac ccgaaagcgt ttcggacagt ccgattcaaa tctttttctc
    34321 gcaacaggat tgacacatgg aaaactcatt gaaagaagcc gccctcaagt tccacgaatt
    34381 acccgttccg ggcaaaattt ccgttacccc gaccaaatct ttggcgaccg acaaagattt
    34441 ggcgttggcg tactctccgg gcgtagccgc tccttgtatg gaaatccatg ccgatccgca
    34501 aaatgcctac aaatacaccg ccaaaggcaa cttggtcgcc gtcatttcca acggtacggc
    34561 cgttttgggc ttgggcgaca tcggcgcgct ggcgggcaaa cccgtgatgg aaggtaaagg
    34621 cgtattgttc aaaaaattcg ccggtgtgga cgtgttcgac atcgaaatcg atgaaaaaga
    34681 cccgcaaaaa ctcgtggaca tcatcgccgc tttagagccg accttcggcg gcatcaacct
    34741 cgaagacatc aaagcacccg agtgtttcta catcgaacgc gaattacgca aacgctgcaa
    34801 aatccccgta ttccacgacg accagcacgg cacggccatc attaccgccg ccgccgtatt
    34861 gaacgccctg cgttttaccg gccgtaaaat cgaagaagcg actttggtgt gttccggcgc
    34921 aggtgcggcc gcgattgcct gcttgaacca attgctggat ttgggcttga aacgcgaaaa
    34981 cgtgaccgtt tgcgactcca aaggcgtgat ttaccaaacc cgcgaagaca aagaccgcat
    35041 ggacgaatcc aaaaaattct acgccgttga agacaatggc cagcgcgtac ttgccgatgc
    35101 cgtcaaaggt aaagacatct tcttgggcct ctccggcgcg aacctgctga cccccgaaat
    35161 gttgaacacg atgaacgaaa aacccatcgt gttcgccatg gccaacccga atccggaaat
    35221 cctgccgccg ctggcgaaag aaacccgtcc ggacgtggtt atcggtaccg gccgctccga
    35281 cttcccgaac caagtgaaca atgtattgtg cttcccgttc atcttccgcg gtgcgttgga
    35341 tgtcggcgcg acgaccatca acgaagaaat gaaacgcgcc tgcgtgtatg ctttggcgga
    35401 tttggcgatg gaagaagtaa ccgaagaagt ggttgccgct tacggtaaga aatttgaatt
    35461 cggcgcggaa tacctgattc cgactccgtt cgattcccgc ctgctgccgc gcgttgctac
    35521 ggctgccgcc aaagcagcga tggaaagcgg cgtggcaacc cgtccgattg cagatttgga
    35581 agcttacgct gccaagctga gcgaatggaa gctgtaagcc gtttgcggtt taaaatgccg
    35641 tctgaactgt tttcaggcgg cattttgctg tcagattgat atagtggatt aacaaaaatc
    35701 aggacaaagc gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgct
    35761 tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgctgt actggttttt
    35821 gttaatccac tataaatgaa agatactgga aaatgaaaga gatgaaacct gtccgttatc
    35881 atattggcga tatgcccgag acttcaaaac aaaccgcctc ccgtcatgac gacagggcag
    35941 tgggtgttga cgatgattgt tttcatgatt cctttggtca atttttgttt gggtgttcgg
    36001 cagaggcaac ccgaaccgcg ccaatttctg taaagcgcag ttgcttattt acctgattgg
    36061 ttcgcttatc ggtttggtct tcgcgttgtt tataggtggg tctgtatcgg gtacgcatga
    36121 ttaatgcccc gggctgattt tgcttcgagg atttgtatcg aatatgccga attgtttcaa
    36181 atttcatact gttatcgaac ggcattggca aaaaccttat ccggttttgt cttttctgct
    36241 taagccgctc tccgggctgt ttgccaaaat tgcggcaaaa cggcggacgg attttttatc
    36301 gggaaaacgg caaagcgaaa agctgcccgt gcctgtggtc gtggtcggca atattcacgc
    36361 gggtgggacg gggaaaacgc cgattgttgc cgcgctggtg tcgggtttgc aggaaaaggg
    36421 cgtcaaggtc ggcatcatca gccggggcta cgggcgcaag agcaaggcgg ttcatgtatt
    36481 gaatgctgag agccgagcgg aagatgcggg cgatgagcct ttgctgctgt tccgcaaaac
    36541 cggtgcgccg acggcggtgg gcagcagccg tgcagaggca ggcagggcgt tgctggcggc
    36601 gcatcccgac atcggactga ttgtggcgga cgacggtttg cagcattacg ccctgcggcg
    36661 agatgtggaa atcgcggtgt ttccggcggc ggatacgggg cgcacggatt tggatttact
    36721 gcccaacggc agtttgcgcg aacctttgtt gcggctggat tcggtggatg cggtcgtcgt
    36781 cagcggcggc aaggcggatg cgctgtttag gccgtctgaa aatatgtttc acagccgtat
    36841 cgaagcggga cggatttacc gtttgaacaa tccgtccgaa atactggaca caggccgtct
    36901 gaaaaatcaa accgtcgtcg ccgtggcagg tattgccaag ccggcgcgtt ttttcgattc
    36961 gttgcggaat atgggcatta ccgtgaagcg aaccgtcgcg ctgcccgacc acgccgacat
    37021 ttcggcggca gatttgcccg atgcggacgc ggtcattatt acggagaaag atgcggtcaa
    37081 attttcagac ggcatttgca ccgataatgt ttgggtgttg cccgtttgtg cgataatcga
    37141 acctgatttg gcggcgtttg tgttggagcg gttggaagat gtaccgaagg ccgtctgaaa
    37201 gcacggtttg ggcggagtga ttacggat
//
LOCUS       DAXIHS010000008        37169 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXIHS010000008 DAXIHS010000000
VERSION     DAXIHS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.008"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(139..1254)
                     /locus_tag="ACFOF2_000331"
     CDS             complement(139..1254)
                     /locus_tag="ACFOF2_000331"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC7106712.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKA
                     AKAAKVAKVAKVAKVAKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVAD
                     NEVITRRRLAEAVAEAKANLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASE
                     AEIDAVVAQNPALKNLSPAQRRELADNIIAEKVRQQAVMQNSRVGEAEVDRFIEQAQK
                     QGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQ
                     DASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTP
                     QERIRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
     gene            complement(1359..3767)
                     /locus_tag="ACFOF2_000332"
     CDS             complement(1359..3767)
                     /locus_tag="ACFOF2_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC7106713.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLYATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            3837..4841
                     /gene="amgK"
                     /locus_tag="ACFOF2_000333"
     CDS             3837..4841
                     /gene="amgK"
                     /locus_tag="ACFOF2_000333"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC7106714.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(4898..5596)
                     /locus_tag="ACFOF2_000334"
     CDS             complement(4898..5596)
                     /locus_tag="ACFOF2_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC7106715.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMHTID
                     LLADKVREEQKAAQ"
     gene            5623..5814
                     /locus_tag="ACFOF2_000335"
     CDS             5623..5814
                     /locus_tag="ACFOF2_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106716.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGSFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            5814..7352
                     /gene="murJ"
                     /locus_tag="ACFOF2_000336"
     CDS             5814..7352
                     /gene="murJ"
                     /locus_tag="ACFOF2_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC7106717.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            7485..8441
                     /locus_tag="ACFOF2_000337"
     CDS             7485..8441
                     /locus_tag="ACFOF2_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC7106718.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLTVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     AVSAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8490..9272
                     /gene="trpC"
                     /locus_tag="ACFOF2_000338"
     CDS             8490..9272
                     /gene="trpC"
                     /locus_tag="ACFOF2_000338"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC7106719.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLHALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADNIEAEVGKLF"
     gene            9337..11637
                     /gene="recQ"
                     /locus_tag="ACFOF2_000339"
     CDS             9337..11637
                     /gene="recQ"
                     /locus_tag="ACFOF2_000339"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC7106720.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMCEGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(11780..12181)
                     /locus_tag="ACFOF2_000340"
     CDS             complement(11780..12181)
                     /locus_tag="ACFOF2_000340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106721.1"
                     /translation="MYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDGYDVYRQLR
                     WQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELKAFPCLSSL
                     FLTKWHKNLYDSGLTKTSTALPRLSSKRTIL"
     gene            complement(12993..13781)
                     /locus_tag="ACFOF2_000341"
     CDS             complement(12993..13781)
                     /locus_tag="ACFOF2_000341"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7106722.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAESIVPVLQNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(13783..14526)
                     /gene="bioH"
                     /locus_tag="ACFOF2_000342"
     CDS             complement(13783..14526)
                     /gene="bioH"
                     /locus_tag="ACFOF2_000342"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC7106723.1"
                     /translation="MPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            14525..15250
                     /locus_tag="ACFOF2_000343"
     CDS             14525..15250
                     /locus_tag="ACFOF2_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC7106724.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCAADLAAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            15315..15779
                     /gene="trmL"
                     /locus_tag="ACFOF2_000344"
     CDS             15315..15779
                     /gene="trmL"
                     /locus_tag="ACFOF2_000344"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC7106725.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            15790..15912
                     /locus_tag="ACFOF2_000345"
     CDS             15790..15912
                     /locus_tag="ACFOF2_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC7106726.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            15909..16628
                     /locus_tag="ACFOF2_000346"
     CDS             15909..16628
                     /locus_tag="ACFOF2_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC7106727.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSALV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRHYEYVVKMGPFSSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            16690..17016
                     /locus_tag="ACFOF2_000347"
     CDS             16690..17016
                     /locus_tag="ACFOF2_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC7106728.1"
                     /translation="MNIKHLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            17098..17682
                     /gene="ruvA"
                     /locus_tag="ACFOF2_000348"
     CDS             17098..17682
                     /gene="ruvA"
                     /locus_tag="ACFOF2_000348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC7106729.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            17682..17819
                     /locus_tag="ACFOF2_000349"
     CDS             17682..17819
                     /locus_tag="ACFOF2_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106730.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            17845..19701
                     /locus_tag="ACFOF2_000350"
     CDS             17845..19701
                     /locus_tag="ACFOF2_000350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC7106731.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            19706..20629
                     /gene="rsgA"
                     /locus_tag="ACFOF2_000351"
     CDS             19706..20629
                     /gene="rsgA"
                     /locus_tag="ACFOF2_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC7106732.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            20876..21100
                     /locus_tag="ACFOF2_000352"
     CDS             20876..21100
                     /locus_tag="ACFOF2_000352"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC7106733.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            21084..21980
                     /locus_tag="ACFOF2_000353"
     CDS             21084..21980
                     /locus_tag="ACFOF2_000353"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC7106734.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(22089..22388)
                     /locus_tag="ACFOF2_000354"
     CDS             complement(22089..22388)
                     /locus_tag="ACFOF2_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC7106735.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(22500..23945)
                     /gene="nuoN"
                     /locus_tag="ACFOF2_000355"
     CDS             complement(22500..23945)
                     /gene="nuoN"
                     /locus_tag="ACFOF2_000355"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC7106736.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVG
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARLAAGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(23955..25451)
                     /locus_tag="ACFOF2_000356"
     CDS             complement(23955..25451)
                     /locus_tag="ACFOF2_000356"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC7106737.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGEIRNPHVADM
                     KDINCREFAVLAVLAAAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(25561..26724)
                     /locus_tag="ACFOF2_000357"
     CDS             complement(25561..26724)
                     /locus_tag="ACFOF2_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC7106738.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            complement(26908..28932)
                     /gene="nuoL"
                     /locus_tag="ACFOF2_000358"
     CDS             complement(26908..28932)
                     /gene="nuoL"
                     /locus_tag="ACFOF2_000358"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC7106739.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(28933..29805)
                     /locus_tag="ACFOF2_000359"
     CDS             complement(28933..29805)
                     /locus_tag="ACFOF2_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFC7106740.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKPFLIKNILFIGI
                     VFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIVLETAYLKTGRSASMIAYGTIF
                     SLIAISIYILLVQI"
     gene            complement(30100..30405)
                     /gene="nuoK"
                     /locus_tag="ACFOF2_000360"
     CDS             complement(30100..30405)
                     /gene="nuoK"
                     /locus_tag="ACFOF2_000360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC7106741.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            complement(30402..31073)
                     /locus_tag="ACFOF2_000361"
     CDS             complement(30402..31073)
                     /locus_tag="ACFOF2_000361"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC7106742.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(31085..31564)
                     /gene="nuoI"
                     /locus_tag="ACFOF2_000362"
     CDS             complement(31085..31564)
                     /gene="nuoI"
                     /locus_tag="ACFOF2_000362"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC7106743.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(31645..32721)
                     /gene="nuoH"
                     /locus_tag="ACFOF2_000363"
     CDS             complement(31645..32721)
                     /gene="nuoH"
                     /locus_tag="ACFOF2_000363"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC7106744.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(32724..34985)
                     /gene="nuoG"
                     /locus_tag="ACFOF2_000364"
     CDS             complement(32724..34985)
                     /gene="nuoG"
                     /locus_tag="ACFOF2_000364"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC7106745.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFSYEGLYHESRLKK
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(35275..35712)
                     /locus_tag="ACFOF2_000365"
     CDS             complement(35275..35712)
                     /locus_tag="ACFOF2_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106746.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(35712..37013)
                     /gene="nuoF"
                     /locus_tag="ACFOF2_000366"
     CDS             complement(35712..37013)
                     /gene="nuoF"
                     /locus_tag="ACFOF2_000366"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC7106747.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
BASE COUNT         8896 a        10362 c         9574 g         8337 t
ORIGIN      
        1 taaattcctg ttacttttcg ttgctagatt cccgcctacg cgggaatgat gaatgacggt
       61 gtaaaagtaa ctcgaaatcc aaaaagccat accgcccgga tttttgccga tcggtatggc
      121 tttttgcttc aaaccgcctt agcggatgtc gacatacgcg ccggaatgca ggtcacgcaa
      181 caggttgacg gttgcctgtt cggctttttg ttggaagatg tattgccgca cggaattgcg
      241 gatacgttcc tgaggtgtgc cggcatcgcg cacttcgttc aatttgatga tatgccagcc
      301 gaattgggtg cggacgggcg cgccgacctg tccgggtttg agcgcgtgga cggcttcttc
      361 aaaggcggga accatcacgc cgtcggcaaa ccagcccaaa tctccgccgt tgcccgcgct
      421 cgcgtcttgc gaatattggc gcgctaggct ggaaaagtct gtgccgctgc gggcctctcc
      481 gtagattttg cggatggtgc tttccgcgcc gacggcggcg ttttcgctgt cggccttaat
      541 caggatgtgt tgggcgcggt attggcgcaa cggtgcgcct tcgggcaggg tgatgccttg
      601 tttttgcgcc tgctcgataa agcggtcgac ctctgcctcg ccgacccggc tgttttgcat
      661 cactgcctgc tggcggactt tttcggcaat gatgttgtcg gcaagctcgc ggcgttgggc
      721 ggggctgagg tttttgaggg cgggattttg tgcgacgacg gcatcgattt ccgcttcgct
      781 tgcctgaatg ttgcggcgtt tgcccgcctg tacgatcagg gattggttga caagctgcat
      841 cagcacctgt cgggacagct cggattcgct tatctgcgcg tctttgggca ggttggcttt
      901 ggcttcggca acggcttcgg caagccggcg gcgcgtgatg acttcgttgt cggcaacggc
      961 ggcaatgccg tctgaaaagc ggataccgcc ctgctgttgt gcgggtgcgg cttctttttg
     1021 cgccgtggcg gcaactttgg caactttggc aactttggca actttggcaa ctttggcaac
     1081 tttggcaact ttggcaactt tggcaacttt ggcagctttg gcagctttgg cagctttggc
     1141 agctttggca gctttggcag ctttggcgga tgcggttttt gccttttgcg gtgcggcgtg
     1201 gacatcggct gctgccagca atgcggcggc aatcatcagg gctttgattt tcatcattct
     1261 ttccttatcg tttgaatcct gcctttgatg gcgggtttgg ggttgtcggt ggaatgccgt
     1321 cttgtgcggg aacggtgctc ctgccgggtt tccgacggtc agggccgttt gttgcgtccg
     1381 gcggaaagag agtgggcggg gatgtagccg ggaacggcga catccatcct gtctgcgggg
     1441 tttctgccga cactgctgag gtctttcaac tgaagtgaga aaaagacagc gtttttgtag
     1501 gtgttttcgc cggtaacgta gcgttgggcg tacacgcccg cgccccagca gccgcaactg
     1561 cttttgtatt ccgcacccgc cagcacctct atcggttttt tggcttcaaa accgtagttg
     1621 taacggacga cggccgacag gttgcgcgtc agcggccatt gtgcggacag gtcgagctgg
     1681 ctgagtttgt cgtaaaaata ggaaccgtcg gacttcaggt agattttttc gttgcgcccg
     1741 tatttgtagc gggcgttcag cactttgccc tgtgcgggac ggtagcttgc accgacggcg
     1801 tagttctcgg cgcgtttgtc gttttggttg tagtggatgc tgctgtcgag gatgaagcgg
     1861 ctgccgatgc tgccggaggc aaatgccacc cagtcggaac ggttgcgcgg ttttttgccg
     1921 acgctgccgt caagcatcac cgcatcatcc ttgaaataga atttctgacc gatgccggcg
     1981 cggaaacgct cttcccccgt cgcgccgtcc aaaatacggc tttgcacggc ggcggaaagg
     2041 ctgtttgcgg tgttaatcct gtcgttgccg taatagaggt tttcgcgaaa gagctgcccg
     2101 tagccgaagc tgctttccga cgaatcgaaa ttgggcaggt cgttttggga tttggcagga
     2161 atatagttgt agaacaggcg cggctcgagg gtttgcagga ctccgccgcc gaacatccgc
     2221 gtattccgct caaaagttgc gccgctgtcg atgttgacaa tgggcagagt gcggctgacg
     2281 cgtcgggctt cttggctgcc gaagcggttg aggctgtaat aggtggcgta cagtccgagt
     2341 ttgggacgga cgtaacccca gctgttgctg aaatcccatt tgatgtcggg atagacgacg
     2401 aggcggctgc cgtcttggcg gctgtcgtgg ctgaagcggg taaattgtgc ggacacgccg
     2461 atttgcgccc tgccggtgtt tttgcgccaa tcggcggaaa ggcgcggcat cagggcatac
     2521 ggtttgtctt tgtagccgct ttggtttgcc agcgtctggt atttcagaac cgaaaggccg
     2581 gcattcaggc tgccgcccgc cgccctgccg ccataatcca gccatacacg gcggttgagg
     2641 ttgacgttgc cggcgatttc tttgttgccg taaaagtcgc ggtagtagcc gctgtcggag
     2701 acttggttga aatcgacacc cgcctgaagc gtgtcggaaa tgtcgtgccg gtgctgccat
     2761 ttcgcctgat agcggttatt cctgccgctt tttttgtcgt gcggcagcca ggtcaggtcg
     2821 gactggccgg cataatccgg ccgcaggtag cgtacctgcc cgtcaaagac cgcgccgcgt
     2881 tcgccgatca cgccgggcgc gaacgtggca tcgagattgg gggcaaggtt gaaataatag
     2941 ggaacggaaa gggaaacgcc gtccgaaccg gcggacagtg agggaacaag caggccgctt
     3001 ttgcggttgc cgtcaagcgg gaagtccgcc caaggcgtat agaaaagggg aacgccgccg
     3061 aacacgaagg cggcgtgttt ggcaacgcct atgccttttt cccgatcggc ttcgacggaa
     3121 gcggccttga cataccagcc ggcatcgccg gcggaacagg tgttgaattg ggtttccgtc
     3181 agtttgtaat gcccttcgcc caacatttcg gcggtgcggc tgacgctttg cagccgccgt
     3241 ccgccttgtt cggtttccat acggacgttg tgcgcttccc cggtctgctg ctcgagattg
     3301 taggtcaggg tttcgccccg aatcagcgta ccgtcctgtt ggagggcgaa ccggtcgcct
     3361 gcggtaacgg tgtcgcccga ctggtcgtaa tccgcccaat cggtattgag ggtcgtccgg
     3421 ttgcgttcga cgacgacgtt gccttcggca cgcacctgca cctgcgactg tccttccacc
     3481 ttgtcggcaa caatgcgcgt atagtcttcg gggatggatg cttcgccgct gcctcggacg
     3541 gcggcttcgg ttctctcggg gctgccgctt tcgttactgc aaaacaggca ggtcgaaccg
     3601 aggctcaggc tggtaggctg tatgggttcg gacacgctcc gaacgcttcc tccggcggtc
     3661 ggattgtccg tttcctccgc cgcaacggca tcggcggcgg cgcaatgcgt gccgaagcag
     3721 aggcccaatg ccagcaccag tggtttgagt gaaaataaac gagccaaaat cgcccctcaa
     3781 gtcggtttgc cagttagaat agcgtttatt gtaacctgaa atgctttagt actgttatgc
     3841 aacgacaaat caaactgaaa aattggcttc agaccgttta tcccgaacgg gacttcgatc
     3901 tgacttttgc ggcggcggat gctgatttcc gccgctattt ccgtgcaacg ttttcagacg
     3961 gcggcagcgt cgtctgtatg gatgcgccgc ccgacaagat gagtgtcgcg ccttatttga
     4021 aggtgcagaa actgtttgat atggtcaatg tgccgcaggt attgcacgcg gacacggatt
     4081 tggggtttgt ggtattgaac gacttgggca atacgacgtt tttgaccgcg atgcttcagg
     4141 aacagggcga aacggcgcac aaagccctgc ttttggaggc catcggcgag ttggtcgaat
     4201 tgcagaaggc aagccgtgaa ggggttttgc ccgaatatga ccgtgaaacg atgctgcgcg
     4261 aaatcaacct gttcccggaa tggtttgtcg caaaagaatt ggggcgcgaa ttaacattca
     4321 aacaacgcca actttggcag caaaccgtcg atacgctgct gccgcccctg ttggcgcagc
     4381 ccaaagtcta tgtgcaccgc gactttatcg tccgcaacct gatgctgacg cgcggcaggc
     4441 cgggcgtttt agacttccaa gacgcgcttt acggcccgat ttcctacgat ttggtgtcgc
     4501 tgttgcgcga tgcctttatc gaatgggaag aagaatttgt cttggacttg gttatccgct
     4561 actgggaaaa ggcgcgggct gccggcttgc ccgtccccga agcgtttgac gagttttacc
     4621 gctggttcga atggatgggc gtgcagcggc acttgaaggt tgcaggcatc ttcgcacgcc
     4681 tgtactaccg cgacggcaaa gacaaatacc gtccggaaat cccgcgtttc ttaaactatc
     4741 tgcgccgcgt atcgcgccgt tatgccgaac tcgccccgct ctacgcgctc ttggtcgaac
     4801 tggtcggcga tgaagaactg gaaacgggct ttacgtttta aacccaatca aaatgccgtc
     4861 tgaaaaccaa gtttcagacg gcatttttca aacgggctta ctgcgcggct ttttgttctt
     4921 cgcgtacttt gtccgccaaa aggtcgatgg tgtgcatacc ggactcccag tcggcaaatt
     4981 cgactttata tttgccgccg acgataaccg tgggcgtacc gtcgatttgg aaggtttcgg
     5041 tcagctcctg cattttgtcg gcgcgcgcct ggctttcggg ggactcgtag gcggcaagga
     5101 cttttttgcc gtcaaaggcg gtttgttcgc ccagccattt tttgaggact tccggctctt
     5161 gcagcttgat tttttggttg accatcgcat cgaaaatatg gctgttcgcc acatctttgc
     5221 tgtcggcggc agccatatcg acggcggcgg cgaggcgtgc gagcgtcagc atttctttct
     5281 gccagacgac gtgttcggta cgcaggtaca tatcgtcttt aaaagacttg gcgtgtttgc
     5341 ttaaaacagg ttcgaggtgg gcgcagtgcg gacagaaata gccgaaaaat tcaaggactt
     5401 cgactttgcc tgcctgctgt tggggaatcg ggttggcaag gacggtatag ttttgccctt
     5461 cgaccagccc tgccggggcg gcggctgccg aagcggcagg cgcgctgtcg gcggggacgc
     5521 tggtttggac tttgctgtcg cacgcggcaa gggcgaacag ggcggcaacg ccgagggcga
     5581 ggtgtctgga tttcatacgg ctctccgtga tgttggaaac agatgttgga aatcaatcgg
     5641 aatccggcta ttttattgca tttccgcgtg ttgatacagt ttgccgccgg aaaaggaagc
     5701 tttcgtttcg ggaaaccgct tcagacggca tcaaacccga tgccgtctga agcggtttct
     5761 gtcgtacaat acgcgccgtt gccccaaacg ggtacgactg ttgaggaaca atgatgaata
     5821 tgctgggagc tttggcaaaa gtcggcagcc tgacgatggt gtcgcgcgtt ttgggatttg
     5881 tgcgcgatac ggtcattgcg cgcgcattcg gcgcgggcat ggcgacggat gcgttttttg
     5941 tcgcgttcaa actgcccaac ctgcttcgcc gcgtgtttgc ggagggggcg tttgcccaag
     6001 cgtttgtgcc gattttggcg gaatataagg aaacgcgttc taaagaggcg acggaggctt
     6061 ttatccgcca tgtggcgggg atgctgtcgt ttgtgctggt catcgttacc gcgctgggca
     6121 tacttgctgc gccttgggtg atttatgttt ccgcacccgg ttttgccaaa gatgccgaca
     6181 aatttcagct ctccatcgat ttgctgcgga ttacgtttcc ttatatctta ttgatttcac
     6241 tttcctcttt tgtcggctcg gtactcaatt cttatcataa gttcggcatt ccggcgttta
     6301 cgcccacgtt cctgaacgtg tcgtttatcg tattcgcgct gtttttcgtg ccgtatttcg
     6361 atcctcccgt taccgcgctg gcttgggcgg tctttgtcgg cggcattttg cagctcggct
     6421 tccaactgcc ctggctggcg aaactgggtt ttttgaaact gcccaaactg agtttcaaag
     6481 atgcggcggt caaccgcgtg atgaaacaga tggcgcctgc gattttgggc gtgagcgtgg
     6541 cgcagatttc tttggtgatc aacacgattt tcgcgtctta tctgcaatcg ggcagcgttt
     6601 catggatgta ttacgccgac cgcatgatgg aactgcccgg cggcgtgctg ggggcggcac
     6661 tcggtacgat tttgctgccg actttgtcca aacactcggc aaaccaagat acggaacagt
     6721 tttccgccct gctcgactgg ggtttgcgcc tgtgtatgct gctgacgctg ccggcggcgg
     6781 tcggactggc ggtgttgtcg ttcccgctgg tggcgacgct gtttatgtac cgcgaattca
     6841 cgctgtttga cgcgcagatg acgcaacacg cgctgattgc ctattctttc ggtttaatcg
     6901 gtttgatcat gattaaagtg ttggcacccg gcttctatgc gcggcaaaac atcaaaacgc
     6961 ccgtcaaaat cgccatcttc acgctcatct gcacgcagtt gatgaacctt gcctttatcg
     7021 gcccactgaa acacgtcgga ctttcgcttg ccatcggtct gggcgcgtgt atcaatgccg
     7081 gattgttgtt ttacctgttg cgcagacacg gtatttacca acctggcaag ggttgggcgg
     7141 cgttcttagc aaaaatgctg ctctcgctcg ccgtgatggg aggcggcctg tatgccgccc
     7201 aaatctggct gccgttcgac tgggcacacg ccggtggaat gcaaaaggcc gcccggctct
     7261 tcatcctgat tgccgtcggc ggcggactgt atttcgcatc tctggcggct ttgggcttcc
     7321 gtccgcgcca tttcaaacgg gtggaaagct gaccgatgcc gatatccttt ttcggaaaac
     7381 gctttttccg cgcctatatt cgggcgcgca tcccccgcgt ccgcaccagc cggacgggct
     7441 gccgcatcat caatgccgtc tgaaaacaga aaaaccgata ccttatgatt ttgacaccgc
     7501 cggacacacc gtttttcctc cgcaacggca atgccgatac gattgccgcc aaattcctgc
     7561 aacgccccgc acccgcatac cgccgcgagc tgcttcccga cagcacgggt aaaaccaaag
     7621 tcgcctacga cttttcagac ggcatttcgc ccgatgcgcc gctggtcgtg ctgtttcacg
     7681 gtttagaagg aagcagccgc agccattacg cggtcgaact gatgctcacg gtacgcagcc
     7741 gcggttggca cggcgcagtc gtccatttcc gcagctgcgg cggcattgcc aacaccgctc
     7801 cggtgttcta ccacttgggc gataccgccg aaatcgcctt tactttggac acgcttgccg
     7861 cgcgttaccg tgaaatatac gccgtcggcg tatcgctggg cggcaacgcg ctggcaaaat
     7921 atttgggcga acggggcgaa aacgcgctgc cgcaagccgc tgccgccgta tccgcccccg
     7981 tcgatgcaga ggcggcaggc agacgcttcg acagcggcat cacgcggctg ctctacacgc
     8041 gctacttcct ccgcaccctg atacccaaag cacggtcgct ccacggtttt cagacggcat
     8101 ttgccgcagg gtgcaaaaca ctgggcgagt ttgacgaccg tttcaccgca ccgctgcacg
     8161 gctttgccga tcggcacgac tactaccgcc aaacttcctg caaaccgctg ctcaaacacg
     8221 ttgccaaacc gctgctcctg ctcaatgccg tcaacgaccc cttcctgccg cccgaagcgc
     8281 tgccgacacc ggacgaagtg tccgaagccg ttaccctgtt ccaacctgca cacggtggtc
     8341 atgtcggctt tgtcagcagc accggcggca ggctgcacct gcaatggttg ccgcagaccg
     8401 tcctgtccta tttcgacagc ttccgtacaa acaggcgtta acggtttgat gctaatattc
     8461 ccctttccca gacgaatacg gaacccgaca tgaccgacat cctcaataaa atccttgcca
     8521 ccaaagcaca ggaagttgcc gcccaaaaag ccgccgtcaa cgccgaacac atccgcgcac
     8581 ttgccgcaga agccgcgccc gtccgcagct tcatcgattc gatacgcggc aaacaccgcc
     8641 taaacctgcc cgccgtcatt gccgaaatca aaaaggcaag cccgagcaaa ggcctgatcc
     8701 gtccggactt ccgccctgcc gagattgccc gcgcctatga aaacgccggg gcggcgtgtt
     8761 tgtccgtact gaccgacgaa ccctatttcc aaggttcgcc cgaatacctc aaacaggcgc
     8821 gcgaagccgt atcgctgccc gtgctgcgca aagacttcat catcgatgaa tatcaggttt
     8881 atcaggcgcg cgcatggggg gcggatgccg tcctgctgat tgccgcagca ctggaacagg
     8941 aacaattgga acgctttgaa gcggtggcgc acgaattggg catgaccgtc ctgctcgagc
     9001 tgcacgacga aaccgaattg gaaaaatgcc gcaacctgac cacgccgctg tggggcgtaa
     9061 acaaccgcaa cctgaggact tttgaagtct ccctcgacca aaccctgtcg ctgctgcacg
     9121 cgctggaagg caaaaccgtc gttaccgaaa gcggcattac gggtaaggcg gatgtggaat
     9181 ttatgcgggc gcgcggcgtg cataccttcc taatcggcga aacgttcatg cgtgccgaca
     9241 atattgaggc ggaagtgggc aaactcttct aaatcccgat ttcagacggc atattgccgc
     9301 cgaccgatca acatccttac cgagcacgga accatcatgc accgaccgac cgccaaacag
     9361 attctgcacg aagtatttgg ttatcccgaa tttcgcggca ggcaggaggc tgtcatcaat
     9421 actttggcag gcggcgggag tttgacggtg ctgatgccga cgggcggcgg caagtctttg
     9481 tgttaccaga ttcccgcgct gatgtgcgaa ggcgtggcgg ttgtcgtatc gccgctgatt
     9541 gcgttgatga acgaccaagt ggcaaacctg cacgccgccg gcatcgaagc ggcggcagtc
     9601 aacagcggca catcggcaga tggggcgcgc gagattgccg acaagcttgc ccaaggccgt
     9661 ctgaaactgc tttatgtcgc accggaacgc ctagttaccg accgcttttt gcgttttctc
     9721 gaccaacaaa ccgtcagcct gttcgccatt gacgaagcgc actgcgtcag ccagtgggga
     9781 cacgatttcc gccccgaata ccaacagctc ggcatccttg ccgaacgcta tccgaacgtc
     9841 ccgcgcatcg ccctgaccgc taccgccgat gccgccacgc gcgccgacat caagcattat
     9901 ctgcacttgg acaatgcgcc cgaatttgtc tccagcttcg accgtccgaa tatttattat
     9961 caggttatcg aaaaaaacaa cggcaaaaaa caattgctgg atttcatccg caaagaaatg
    10021 acggggcaaa gcggcattgt gtattgccta agccgcaaaa aggttgaaga tgtggcgcag
    10081 tttttgcgtg aaaacggatt aaacgcgatt ccgtatcatg ctggtttgag catggacgtg
    10141 cgcgaggaaa accaacgccg ctttacgcat gaagacaata ttatcgtggt ggcgaccgtg
    10201 gcgttcggca tgggcataga caaacccgac gtgcgctttg tcgcccatct cgatatgccc
    10261 cagagtgtcg aacatttcta tcaggaatcg gggcgtgccg gacgggacgg gctgcctgcc
    10321 gcgagctggc tgtgttacgg tttgaacgat tgggtgttgc tgcgcgaacg gattgccgaa
    10381 ggcaacagcg acgaggtgca aaagcaaatc gaaatgcaaa aactcgatgc catgctttcc
    10441 gtctgcgaaa ccgccgcctg ccgccgcgta ctgctgctca aacatttcgg cgaagaatcc
    10501 gaaccctgcg gccattgcga caactgcctg catccgcccg tacggtttga cggcacggtg
    10561 ttggtgcaaa aattactcag ctgcgtgtac cgcgccggac aacgttttgc cgccggttac
    10621 atcaccaacc ttttgcgcgg caaaagcgac gattggatac gcggcaaccg gcacgaacaa
    10681 ctgtccacct tcggcatcgg tacggagttg tccgacaaag aatggcgcag cgtcatccgc
    10741 cagtgtatca gcctcggcta cctcaccgtc aacattaccc gatatcaggc attgcaactg
    10801 accgaagccg ccaaaaaagt cctcaaaggc gaaaccgaag tgatgctgcg tccgctcaag
    10861 cgcgacaagc ccgccgcccg caccctcaaa gacaactggc tgcgtaccga acgcgaagaa
    10921 cgcctgtggc aggcattgcg cgtttggcgt atgaaacagg cagaagccga agtcatcccc
    10981 gcctatatga ttttcggcga caaaaccctg cgcgaccttg tcgaaaaaat gccgcaagac
    11041 ctcaacggac tgcacgacat ctacggtttg ggcgaagcca aaatcgaccg tttcggacac
    11101 ggcatcctcg aagtctgccg caacaccgcc ggcttcagtc gcgacgcggt catccgtccg
    11161 caaaccgaac gcgaacaaca actgcgtcaa aaactcgagg cctggcggta tgaacaggca
    11221 agggcggaaa actgcgccct gcatgccgtc ctctccgacg aaagccttgc cgatatgctt
    11281 gccgccacgc ccgaaaccga aaccgacctc gaaggcgtac acggcttggg cagcgtacgc
    11341 gccgccaaat acggacggga catcctcgcc gtctgccgtc cgttttcaga cggcatcgat
    11401 gaaaccgcca aacacaaacg ccgcctgatg cgcgccctga tccaatggtg caacgaaacg
    11461 gcaaaacacg aacagtccga accctaccgc attctcagca aagccgccct acgcgccatt
    11521 gccgccaaac agccggaagg tttggcggag cttgccgccg tatatggcgt aggcgaagaa
    11581 aaagccgcac gttacggtgc ggcggtgttg gcggtattgg aacgaaatgc cgtctgaaaa
    11641 ccgttaccaa gtttcagacg gcattgcctc tatttaaaaa ttcctgtttt tatagtggat
    11701 taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt
    11761 cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgccg
    11821 tactggtttt tgttaatcca ctatcatata gatttttatg ccatttggtc agaaacagcg
    11881 aagacaggca gggaaacgct ttcagttcca tcgcgtcttc aaaatcatcc caaacatcgc
    11941 tcaaattctg tttggatatg ccgtattccc gtccggcaaa catcacggtc tttttgcttt
    12001 tggcggcttt tttgaatgcc tttctctgtt tttcgggtat ctgctgccat ctcaactgac
    12061 ggtacacgtc gtagccgtcg ccccaaaaag aggcataccg ttgcgtgtct tcgccgacca
    12121 gcggcgcggt attcccaacc cgtacatatc ccgttggcac gacagcactt gccttataca
    12181 tgtgcagacc gcgttcccaa tacatattca attgaatcgc tcataaattt ttgctccaaa
    12241 atttaaagtt ttactatagt agaaataaat atcaaaactg aaaatattac tgaaaaaatt
    12301 ttatattgtt taacttttct tttatacgaa ttcgcctttg ttgaaattaa ttgataatca
    12361 aaccagcaag aaataattcc ataataaaac attccaatcc aataataaaa ttcaggcctc
    12421 ttagaattag taaaaatttt aaatccataa tctctttcaa aagtagctat tatacaagta
    12481 aacaatatca ccaaccctaa acagtccaac ataaaaatat agtttaattt tttgttcata
    12541 caatatttcc aatattattc tttaagtctt tgtaaagtgg tttcaaatcc gccagctcct
    12601 ccgccccaag cggtttgttg ccctgccagt ccaaagccag cagggtcgca tgtgtttttt
    12661 tgccaccgtg ttcttcaact cgggtaacct ggcaggcagt gtactgcttc agtctgatgt
    12721 tatagacgca atatacgtct ccggcatcag catatagtgg attaacaaaa atcaggacaa
    12781 ggcggcgaag ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac
    12841 cttagagaat cgttctcttt gagctaaggc gaggcaacgc tgtactggtt taaatttaat
    12901 tcactataca tcggcatttc ctttagttct gtcggttttc acaaacaaat gccgtctgaa
    12961 acttggaaac ggcttcagac ggcatctgca catcatctct tcggaaaaaa ctgcaccacg
    13021 ttctcccctt gcggcagcat cagtttttta acggcgtgtc cgtacactgt ttccagcgta
    13081 atactcaaac cgccttcccg ttcaaatatt gtcccgacac acgaacgcgc ggcgttttca
    13141 tcgttccacg acatcgcccg ccaaacgccc agcgtgtcca tatccgccca aaacgtttcc
    13201 gcctttttgt aatccaacac cagcttcgcc gtatcggtaa cggggtcgta aaacccgttt
    13261 tcagcaagca tatcgcccaa gtcgtgcata tcggggaaaa gcgcgctgcg gctttcaatg
    13321 ccgttttctt tcagacggca tttcagttcc gccaaggtat ctcgcccgaa gcaggtaaaa
    13381 aacagcagcc cgtccgtctt cagcgcgcgc gcccaatttt gcagcacggg gacgatgctt
    13441 tccgccgcca acagtccgag attcgaccac aacatatcgg cacacgcttc gggcagcggc
    13501 gcggtcgggg attggcagtg ctgcaccacg cccttacccg caaacctttg ccaaaaaccg
    13561 cctttgcgcg cggctgccgc cgccgccaaa aaatccgcac gggaatcgta ttcttcaaat
    13621 accgcctgcg gatagcgttt cgccagcagg ctgcggctga tgtccgcatc cgccccggca
    13681 agcaggatat gtttgggcgc gttgcggacg agtgtcaggc gttggtcggc atattcggcg
    13741 agatggcggt gaacctgcca gcatttgtcc tgcggattca tgtcaaaccc cttcgacaaa
    13801 gtcgcggtac agcgcggcaa acgcttccgc atggctcaaa aacggcgcgt gtgccgcctt
    13861 ttccatcaca accagcctgc ttcctttcaa atgccgatgc agatactcgc ccatgcgcgg
    13921 cggcgtaatc gcgtctttgc taccgaacac cagcagtacc ggaacatcta tcttgtccaa
    13981 caaatgccgc gcatccgccc tttccgccgc gtccaaagct tcctgtaagg cggagggtgt
    14041 gccgcagcgc gccaaatcgg gcaggattct gcctatgatt ccgtccgcat catgcgtatg
    14101 caaaagctgt aattgtagaa actgtttgat atgtttggca taatccgttc ggaacgcgcc
    14161 gaccattttg cccaatgcag gcgcggcaag cccttcggga tagtcttcgt cagccgtcag
    14221 tcgggcgaaa ctcgccgtca ggcagagcga acggactttg tcgggatggc gcgccgccag
    14281 atacagcgcg accaatccgc cgagcgacca gccgagaatg tcggcgggcg tatcgatttg
    14341 tgcggcaaca gcgtcggcgg ctgcctcaat atcgaagggt tgcgcgaagg gcgcgtcgcc
    14401 gtgtccgggc aaatcgacgg cggacaccgg ccacgttgca ggcagacggg gcatcaggtc
    14461 gtcgaacacg tggcggttcg ccccccaccc gtgtatcagg taaacttttt tggcagcatc
    14521 aggcatgaat tttctctctt gttggcggcg catcgcagac gcgcccacta tcaggcgttg
    14581 cgtattatgc cacggttcgt ccggcgtttc agacggcatc tgcgccggct gcgcggcgga
    14641 tttggcggca taccgcaccg acgcggcaaa cagctgcccc ctgtgtttca gacacatcca
    14701 aggcggcgcg gtgtgcggcg gctgtcagaa aaaaccgccc gcattcgacc ggatgtgggc
    14761 ttcgctgcat tacgaaccgc ccgtcagcaa tatgatacac gcgctgaagc acttggctga
    14821 tttgagcatg gtgcagccgc tggcagacct gatgatgcag aatccgcccg accggcttgc
    14881 agacgaatgt ttcgatttcg tcctgcccgt accgctaagc agggagcggc tgctgcaacg
    14941 cgggttcaac caaagtgaaa gcatcgtcgg gctgctggca caacgctacg gctggcagat
    15001 actgccccga cacaccgttt tccgacacca ccgcccgccg caaagcacgc tcaaaggcgg
    15061 cgaacggcgg cgaaacatca aaaacgcctt tgaaatccgc acaccgatac cggaaaactg
    15121 taatattttg ttaatcgacg atgtctttac caccggcgcg acgctggacg aactggcaaa
    15181 gacgctgaaa aaatcgggcg caaaccgaat ctgctgctgg acgctggcac gcacgccaat
    15241 gaaaaaataa ctaaattttt tgacacccca tccgccttgt ggcaaggtta cgcgcctata
    15301 agtgattgat atttatgttt accatcgttt tataccaacc ggaaatcccg ccgaacacgg
    15361 gcaatatcat ccgcctgtgc gccaataccg gcgcggattt gcaccttgtc aaaccgctcg
    15421 gcttcccatt ggattccgcc aaaatgaaac gcgccgggct cgactaccac gaattcgcca
    15481 gcctgacggt gcacgaaaac ttcgacgact gcctcaaggc actcgcaggc aggcgcattt
    15541 tcgccctgac caccaaaggc acggcgcgcc ccgatgaaac cgcgtttcag aaaggcgatg
    15601 ttttactgtt cgggccggaa acgcgcggac tgcctgccga catcctcgac agcctgcccg
    15661 ccgcgcaaaa aatccgcctg ccgatgcagc ccggcagccg gagtatgaac ctttccaata
    15721 ccgttgccgt gattctcttt gaagcgtggc ggcaacacgg ttacgcaggc ggcgtttgaa
    15781 cgcaagttca tgccgtctga aaacctatcc ggacacattc cgaaccgccg tccgcactgt
    15841 acggcggttc ggaacgtatc cggcgggcat gatgcccatt cgtcttcaac tcaagaacgg
    15901 aacacgtttt gactttaacc cgaaaaaccc ttttccttct caccgccgca ttcggcacac
    15961 actcccttca gacggcatcc gccgacgcag tggtcaaggc agaaaaactg cacgcctccg
    16021 ccaaccgcag ctacaaagtc gccggaaaac gctacacgcc gaaaaaccaa gtcgccgaat
    16081 tcacgcaaac cggcaacgcc tcgtggtacg gcggcaggtt tcacgggcgc aaaacttccg
    16141 gcggagaacg atacgatatg aacgccttta ccgccgccca caaaaccctg cccatcccca
    16201 gctatgtgcg cgtaaccaat accaaaaacg gcaaaagcgt catcgtccgc gtcaacgacc
    16261 gcggtccctt ccacggcaac cgcatcatcg acgtatccaa agccgccgcg caaaaattgg
    16321 gctttgtcag ccaagggacg gcgcacgtca aaatcgaaca aatcgtcccg ggccaatccg
    16381 cactggttgc cgaaaacaaa gacatcttca tcgacttgaa atctttcggt acggaacacg
    16441 aagcacaagc ctatctgaac caagccgccc aaaacctggc ttcatcggca tcaaacccga
    16501 acctatcggt tgaaaaacgc cattacgaat atgtcgtcaa aatggggccg ttttcctcgc
    16561 aggaacgcgc cgccgaagcc gaagctcagg cgcgcggtat ggttcgggcg gtattgactg
    16621 ccggctgacg gttatttgat accttattaa tatccacatt ttccaaccca cgatttacaa
    16681 aggcaaaata tgaacatcaa acaccttctc ttgaccgccg ccgcaaccgc actgatgggc
    16741 atttccgccc ccgcactcgc ccaccacgac ggacacggcg atgacgacca cggacacgcc
    16801 gcacaccaac acagcaaaca agacaaaatc atcagccgcg cccaagccga aaaagcagcg
    16861 ttggcgcgtg tcggcggcaa aatcaccgac atcgatctcg aacacgacaa cggccgtccg
    16921 cactatgatg tcgaaatcgt caaaaacgga caggaataca aagtcgttgt cgatgcccgt
    16981 accggccgcg tgatttcctc ccgccgcgac gactgaattt gatacaatcc gtgccgtctg
    17041 aagcccgaac cggtttcaga cggcattttg cacccgacac ttcaggatcc ttcatacatg
    17101 atcagcagac tgaccggcaa actggttgaa aaaaaccctc cgcaaatcgt catcgatgtc
    17161 aacggtgtcg gctatgaggc cgacgtatcg atgcagacct tctacaacct gccgcccgtg
    17221 ggtgaaagcg tacaactgtt tacccagctt atcattcggg aagacgcaca tcttttattt
    17281 ggttttgcca ctgcggaaga acgcaagacc ttccgccaac tgattaaggt cggcggcatc
    17341 ggcgcgaaaa cggctttggg cattttgtca gcgatgacgg cagacgagct ggcgcgggcg
    17401 gttgcagaag aagatgtcaa acgcctgtct tccgcgccgg gaatcggcaa aaaaaccgcc
    17461 gaacgtatgg tcttggaact gcgcggcaag ctggtcgcgc atacggtaac ggacgggctg
    17521 tttgccgccg cacccgccgc cgacgaaacg gaagacatcg tcagcacact gcttgcactg
    17581 ggttacagcg aacgcgaagc aaaagcggcg gtcaaaggcg ttccgaaagg gacggacgtg
    17641 ggcgaaggcg tgcgccttgc tttgaaaaac ctgctgaaat aatgccgtct gaaggcggcg
    17701 cggcgtttgc cctgacgaaa ccctgcgttt ccgcctcgcg ctgtcttccc gacggctttt
    17761 cccgtaaaat ggcgttattg tccttccttt cagacggcat tgtgtttgat gccgtctgaa
    17821 actgttttac cgaaatcgaa aacaatgttg aacaaaatat tttcctggtt cgagtcccga
    17881 atcgaccctt atcccgaagc cgccccgaaa acgccagaaa aaggcttgtg gcggtttgtc
    17941 tggagcagca tggccggcgt gcggaaatgg atagccgccc tggctgcgct gaccgccggc
    18001 atcggcatta tggaagccct ggtttttcaa tttatgggca aaatcgtgga gtggctcggc
    18061 aaatacgcgc ccgccgaact gtttgccgaa aaaggttggg aactggcggc aatggcggcg
    18121 atgatggtgt tttcggtcgt gtgggcgtat gccgcgtcca acgtgcgcct gcaaaccctt
    18181 cagggcgtgt tccctatgcg cctgcgctgg aacttccacc gcctgatgct gaaccaaagc
    18241 ctcggttttt atcaggacga atttgccgga cgcgtgtccg ccaaagtcat gcagaccgcg
    18301 ctggcgttgc gcgacgcggt gatgacggtt gccgatatgg tcgtttatgt gtcggtgtat
    18361 ttcattacct ccggcgtgat tctcgcctcg ctcgactcat ggctgctgct gccctttatc
    18421 ggctggattg tcggtttcgc ttcggtgatg cgcctgctga ttcccaaatt ggggcaaacc
    18481 gccgcatggc aggcggatgc ccgctcgctg atgaccggcc gcattaccga tgcctattcc
    18541 aatatcgcca ccgtcaaact cttctcccac ggcgcgcgcg aagccgccta tgccaagcag
    18601 tcgatggaag aatttatggt tacggtgcgc gcccaaatgc ggctggcgac gctgctgcat
    18661 tcgtgcagct tcatcgtcaa cacctccctg accctctcca ccgccgcact gggcatctgg
    18721 ctctggcaca acgggcaggt cggcgtgggc gcggtcgcca ccgccaccgc gatggcgttg
    18781 cgcgtcaacg ggctgtcgca atacattatg tgggaatccg cgcggctgtt tgaaaacatc
    18841 ggcaccgtcg gcgacggcat ggcaaccctg tccaaaccgc acaccatcct cgacaagccg
    18901 aacgccctgc cgctgaaagt gtcgcaaggc gaaatcaaat ttgaacacat cgatttctgc
    18961 tacgaagccg gcaaaccgct gctcaacggc ttcaacctca ccatccgccc gggcgaaaaa
    19021 gtcggcttga tcggacgcag cggcgcgggc aaatccacca tcgtcaacct gcttttgcgc
    19081 ttctacgaac cgcaaagcgg cacggtttcg atcgacgggc aggacataag cggcgttacc
    19141 caagaatctt tacgcgccca aatcggtttg gtcacgcaag atacctcgct gctgcaccgt
    19201 tccgtgcgcg acaacattat ttacggccgc cccgacgcga ccgatgccga aatggtttcc
    19261 gccgccgaac gcgccgaagc cgccggcttc atccccgaac tttccgatgc caaagggcgg
    19321 cgcggctatg acgcgcacgt cggcgaacgc ggcgtgaaac tctccggcgg gcaacgccag
    19381 cgcatcgcca ttgcccgcgt gatgctcaaa gacgcgccca tcctgctgct cgacgaagcc
    19441 accagcgcgc tcgattccga agtcgaagcc gccatccagg aaagcctcga caaaatgatg
    19501 gacggcaaaa ccgtcatcgc catcgcccac cgcctctcca ccatcgccgc aatggacagg
    19561 ctcgtcgtcc tcgacaaagg ccgcatcatc gaagaaggca cacacgccga actcctcgaa
    19621 aaacgcgggc tttacgccaa actctgggcg caccagagcg gcggcttcct caacgaacac
    19681 gtcgagtggc agcacgactg aaccgatgcc gtccgaacac ccgttttcag acggcatttc
    19741 cacacccaac cccaaagaaa ccatgaacga caccgcccaa attaccgcca gctacggccg
    19801 ccgctacatc gtgcgcacgc ccgacggcac aacctacgaa gccagcaccc gcaaaaaacg
    19861 cgtcgatttc gcctgcggcg accgtgtccg catcagcccc gtcaacgccg aacaagttgt
    19921 gattgaagat tttttaccgc gccaaagcct gctctaccgc caagacgcgt ggaaaaccaa
    19981 actcatcgcc gccaacgtta cccaactcct catcgtaacc gccgccgtcc cgagtccgag
    20041 cgtgcggctg ctgcaacgcg ccctgcttgc cgccgaagcc gccggtattg aagccgtcat
    20101 cgtcctgaac aaagccgacc tgcccgaaac cgccctttgg cgcgaaaaac tcaaattcta
    20161 cgaaacgctg ggttatcccg tcatcgaaac ccgcgcactg gaaaacgccg gcagcctgcg
    20221 ccccgccctg caagggcaca gcaacatcct gctcgggcag agcggtatgg gcaaatccac
    20281 cctgaccaac gcccttttgg gcagccaaac cgcccgcacc ggcgacattt ccgccgcact
    20341 cgactcggga aaacacacca ccacccacgc ccggctttat gatttgaacg gcgaaaccca
    20401 actcatcgac tccccgggtt tgcaagaatt tggtttacac cacctccaag ccgccgattt
    20461 gccgcgctat ttccccgatt tccgccacct tgtcgggcaa tgccgcttcc acaactgcac
    20521 ccaccgcgcc gaacccggct gcgccttcaa agccgccgcg caaaccgggg cggcaagccc
    20581 cgaacgcctc gcctttttgc agggcatcac cgacgaactg cccgggtaac gccttgccgc
    20641 ctgggcggaa aaaatgccgt ctgaagccgg attcgggttt cagacggcat ccgtttttca
    20701 aaaatgctac aatccgcttt ttaccggaac atccgaaact atgttccaac acacaggacg
    20761 gcacataaag caccgcccta tgtattgccc tgatttggaa agggttacac ccctcccaaa
    20821 taaagtctga tcctgccgcc ctaaagggcg gggtttcaac cgaaaaggaa atacgatgaa
    20881 gaaaaacgcc ccgaaatcgt ttgaagaagc cttgtcgcgc ctcgaatcgc tgacgcagtc
    20941 tatgcagggc gaaatgccct tggaagacgc gcttgccgcc tatcaggaag gcaacgagct
    21001 ggtcaggtac tgccaaacca aactggcaca agtcgaacag aaattacagg ttttggatgc
    21061 ggacgggctg aaggagttga accttgaatc cgacgaatga tttgaaagca tggcagctta
    21121 gggcgcaggc gcaaacagag ctgctgcttg aacggttttt accgtctgag ggggaaatac
    21181 cgcacacact gcacgaagcc atgcgttatg cggctttgga cgggggcaaa cgtctgcgtc
    21241 cgatgctggt gctggcggct tcggaattgg gcggagccgt ggcggatgcg gtcggacagg
    21301 caatggcggc aatcgaaatg atccacgtct attctttggt tcacgacgat atgccggcga
    21361 tggacaacga cagcctgcgg cgcggcaaac cgacttgcca tatacaatac gacgaagcca
    21421 ccgcgctact ggtcggcgac gctttgcaaa cccaagcctt cgacgtgtta agccgtccga
    21481 cagaactgcc cgcagcacgc cagttggcaa tgttgtcggt gttggcgaaa gcgggcggca
    21541 gcgcgggcat ggcgggcggt caggcaatcg atttggcgaa tgtcggcaaa caaatggttc
    21601 aagccgattt ggaacagatg cacagcctga aaacgggtgc gctaatccgt gcggcggttt
    21661 tattgggggc gatgtcctgc ccgaatctgt ctgaagacag tctggcggta ttggacgatt
    21721 atgcggcaaa actggggctg gcgttccaag tcattgacga tgtgttggat tgtgaagcgg
    21781 acacggcgac tttgggcaaa acggcgggca aagacgcaga caacgacaag ccgacttatg
    21841 tgaaactgat gggcttggaa gcggcgcgct catacgcaca caaactggtt gccgaagcgg
    21901 tcgcgctgct cgaacccttc ggcgacaaag ccctgcgcct gcggcagttg gcagaatttg
    21961 cagtcgcccg caaatattaa aaccggcgta tatcgccggc tgaaacgtgc cggcacagcg
    22021 atgccggcct gcacttgaat gaaacaaatg ccgtctgaaa gggtttcaga cggcattcct
    22081 atgcctgcct attccctgtt gcgcgtgtgc caaaaatacc gccacacgaa acagggaaac
    22141 gcaaaaatca ggtacaggca gacgacggtg gcgtagaact cccaaccctg attgtgtacc
    22201 gctcccgcac gggattcgag gatgtaggca agaacggcgg tcagcgcgaa acctgccgcc
    22261 agctcgatca agtggtgtcc gaaatgtttg cgcttgagcg cggccacgcc gaacagtctg
    22321 gtcgtgagga agggggcgtt ggcaaagatg agtgccaaga ccaaaaggat gtacatggat
    22381 gcggtcatgg tgggctttct gtttgggcgt tgtcggcggc gatgccgtct gaactgcatt
    22441 gcggaacggc atatcttaac aaaaacggca gcctctgaca cggctgccgt tgcggcggct
    22501 tacagcgtgt tttccaacgc cttggcgcac cagtcgataa cggtttgcgg cataatgccc
    22561 cacagcacca acagcagcgc gttgacgctc aagaggaatt ttgcggcatt gttgccgccg
    22621 gcggcgagac gggcgcggcc ggattcgtca aaatacatga ctttgaccac gcgcaggtag
    22681 tagaacgcgc caatcagcga catcacgacg gcaaacaccg acaaccaaac atagccttgt
    22741 tttaagagtg ccataatcac gccaaatttg gcgtaaaaac ccatcagcgg cggaatgccc
    22801 gccatagaga acataaccag taacatcaaa aaggcaagcc atacgcggtg ttggttcagc
    22861 cctgccaaat cgctgatgtt ttcgcactcg ttgtccccat ccgacaacac catcaacacg
    22921 ccgaaccctg ccgccgccat cagcgcgtag gtaatggcgt aatagagacc cgccgcaaag
    22981 ccgaccgcgc ccgccataaa cgccaacagg atgaaaccca tatgcgatac ggtggaatag
    23041 gcgagcatac gtttgatatt ggtctgcatg atggcggcaa ggttgccgac cagcagcgag
    23101 gcggcggcaa gcagggcaaa catcagagac cagtcatgat gcacggttcc cagcccggta
    23161 acgaggatgc ggaaagcgaa aacgacggcg gcgactttcg gggcagtgcc gactagggcg
    23221 gtaacagaag tgggcgcgcc gtgatacacg tcgggcaccc acatatggaa cggtaccgcg
    23281 ccgagtttga acccaacggc gacgacgata aacaccagac ccagtttcaa cagccattcg
    23341 ttggcttctt cattgaagga agaggcaagc acgccggcaa attccagcga accggttgcg
    23401 ccgtaaacca tagaaatacc gtagagcagc aggccggatg ccagcgcgcc tagaacaaaa
    23461 tatttcaagg cggcttcggc ggcaaagccg gaatcgcggc gcagggcaat cagggcgtaa
    23521 agggcaagcg acaagagttc caaaccgata taggcagtta aaaaatgccc cgcgctcacc
    23581 atcacgccca tacccaagag ggcaaacagc gacagggtgt aaaactcgcc tttgaaaata
    23641 ccgcgcactt ggttgtaggg cctggcatag acaaacaggg caaaggtcaa ggcatataaa
    23701 accatttttg ccaaacgcga cataccgtct gcaatataca tgccgttgaa cgaagacgtg
    23761 ctgccctgtt cccaaacggc aagctgcacc acagccgtaa ccgccaccgt tgccaacgcg
    23821 ccgtaatgcg tccaacggcg tttgtcatca ctgacccaca agtctgccag caacaataac
    23881 accagcagcg acagcagcac gatttcgggc atggcgggca ttaaattcaa atcagaccag
    23941 ttcatttaca cacctcaaat cttgctttgt gccacatggg caatcaaatc gtttgccgcc
    24001 tgatgtacca cttcgataaa cgcgttcgga tacaggccca tacccaaaac agccgccgcc
    24061 aaaaccgcca aaaccgcaaa ttcgcggcaa ttgatgtctt tcatatcggc aacgtgcgga
    24121 ttgcgaatct cgccgaaaat cacgcgtttg tacatccaca gggtataaga cgcgccgtaa
    24181 atcagcgtca tagccgccaa cgcgcccacc cagaaattca ctttaaccgc gcccataatc
    24241 accataaact cgcccacgaa gccggaagtc gccggcaaac cggcgttcgc cataccgaac
    24301 aacatcataa acgccgcaaa cttgggcatc acgttgacca cgccgccgta atcggcgata
    24361 ttgcgcgtgt gcaggcggtc gtacatcaca ccgatgcaca taaacatcgc cgccgacacg
    24421 aaaccgtgcg aaatcatctg aatgaccgcg cctttcaatg cccagtcgtc caactgcccg
    24481 tcaacaaaca aaaacatccc aagcgttaca aaacccatat ggctgatgga cgaatacgcc
    24541 accagttttt tcatatcggt ttgcaccaaa gccaccatac cgatataaat cacggcaatc
    24601 agactcaata cgatgatcac gggggcaaaa tagcgtgccg catccggcat aatcggcagg
    24661 ataaagcgca agaaaccata cgcacccagt ttcagcgtaa tcgcagccaa caccatcgaa
    24721 ccgccggtcg gtgcttcaac gtgggcatcc ggcagccaag tgtgtacggg gaacatcggc
    24781 acttttacgg caaatgacag gaagaatgcc acaaacaaaa gctgctgtac gcccaacgga
    24841 atctgtttga tgttttggaa atcgacaata gagaagctgc ctgtttgata gtacaggtaa
    24901 accatggcaa ccagcatcaa gagtgaaccc atcagcgtat agaggaacag cttgaccgac
    24961 gcatagacgc ggcgcgggcc gccccataca ccgataatca ggtacagcgg aatcagcata
    25021 ccctcaaaga acacataaaa cagaatcgca tcctgagcgg caaacgcgcc gttaatcaaa
    25081 cccgacatca tcaggaaagc ggccatatac tgcgccggac gtttctgaat gacttcccaa
    25141 ccggccaata ccaccagcag cgtaataaac gcattcaaga tgataaagag cactgaaata
    25201 ccgtccacgc ccaatgcata gttgattttc agcagcggaa tccactcgtg gaactcggta
    25261 aattgatagc cgccgctcaa acggtcgaaa ccggtaaaca ggggcagcgt taccaagaaa
    25321 ccggcaagcg cacccataaa ggcaagcaca cgcgccagcg gcgcacggct gtccttaccg
    25381 gtcgacaaaa ccagcacacc tgcggcgatg ggtatccata ttgccaagct gagtaggtag
    25441 ttggaaaaca tagtggttaa cctgtggtta aaataaaaat atcgtagtgc gggttagttt
    25501 gtcagacgac gtttcaggag gtcgtctgaa aaagggatga atactctctc ccctctttac
    25561 tcactcccaa tcaatatctg tactatcgcg atattctgcc tgcactaaaa catctaattc
    25621 agccaaattt tttatttctg actgcattgc tagtaaagct cttaacttca tacttaaatc
    25681 aatatatcta gagccttcac ttgctccttt tgcagactta aatttatttt tttctaaaaa
    25741 ttttttaata ggagcgttta acactggata tctatctgga tattcttgac aaagcatttc
    25801 agaaaaaact gaaccacgag tggaaactcc acattctttt agccaatcaa tttgcttggc
    25861 aacaacatta tctctctcag caagtggtgc atcaaaaact gattggatag caatgcataa
    25921 ttttctaaaa tctgaagact ttccggtcct tttccacgtt tgatcttgaa atcgattacc
    25981 taccccttct cctttatttt cgcttttcca agaccataat ttattaaatt ccacataaaa
    26041 ctcatcattg tctatctttc ctgcagcaca ttgcttaata agacttttta attgattaca
    26101 cacagtttga tggtttctca tctgttttct ccgtgctgca atcagttctt gattataatt
    26161 tggcatttcc ataaataact tatccatcaa tgattggcta acagttgatt tcttataatt
    26221 aatttcagct tcctgatact cctcaagcca atcttccgat actgggatag atttcatcgc
    26281 aatttcatca gcccaagatt tgacttttat aaaatcctgc tcggaaattt tagtcaaaat
    26341 atttgcttca taattgctat taaacgcagc atgagtcaaa tttgaagaac cgattaaagc
    26401 ataatattca ttcttatcat tcttccaaaa tacagcttta ggatgaaaac cctgaatgcc
    26461 ttgagctacc atcagtgtga atttcaaatg ggacggaagc catttcaaca ctttccgaat
    26521 agcctctttt ttagacaatc cgaaatcttg tccaaaaata aatttaaact cttgcaattt
    26581 gctattgagc ttatttttta catcccaatg agtgagatac gcagagaaaa tatacagctc
    26641 tactgcctct gaaacagctt tctcatatat tgaaatcagt tccttctcat ttggtgtatc
    26701 tgaatgtaaa aataactgtg tcatcccttt tgactcctct atatttgttg agattgccta
    26761 aaaaatttag gaaacagata atttgcatgt tggctatttc acgcctgttg ggtaccattt
    26821 tgttccaccc tttgacttaa tgcccacttc tcaaactttt cagacgacct ctacaaaact
    26881 atgaacaggt cgtctgaaac tccgccttta tctgaacaat ccccagaagg tcatgccgag
    26941 cagtaccaat acgccgaata ccatagcggc ggcgtaggtg tagataaagc cggtttgggc
    27001 tttacgcact tgcgcggcaa tcgcgccgac cagtttggca gagccgttga cgatgccgtt
    27061 atcgataatg gcggtatcgc cgactttcca gaagaaggta cccaatgcgc gtgtgccttt
    27121 ggcgaaaacg ttgaaataca gggcgtcgag gtagtatttg ttttcaaaca aaacgtaaat
    27181 cggacggaac gtctgtgcaa ttttcgctgg caggtgcggc agtttgacgt acaaaagcca
    27241 tgcgctcaac acgcctgcga tagcaaggta gagtacgggc gaatgcaggc tgtgcgacac
    27301 cattgccaat gcgccgtgga actcttcctt catgatgtgc atggtcggat gcgcgtcggt
    27361 attgacgaaa atcacatctt tgaagaaatc gccgtaaagc ataggctcga tggcaaggta
    27421 gccgatgatg acggacggaa tggcaagcaa aatcaaaggc agggtaacaa ccaacgggct
    27481 ttcgtgcgga ttgtcgtttt tgcccaagct gtgatgttct tcgccatgat gatcgtcatg
    27541 atgttcaggc aagctgcgcc atttctcttc gccgtggaac accataaagt attggcggaa
    27601 tgcgtagaac gcagtaacaa aaacgctggc gaggacggca aaataggcaa agccgctgcc
    27661 cggcagggtg ctgtatttcg ccgcttcgat aatcgaatct ttggaataga agccggagaa
    27721 gaacggcgta ccgatcagcg acaagttacc gatcagcatg gtcagccaag taatcggcat
    27781 gtattttttc agattgccca tgtggcgcat atcttggtca tggtgcatac cgataatcgc
    27841 gctgcccgcc gccaagaaca gcaaggcttt aaagaaggcg tgggtcatca catggaacat
    27901 cgccacggaa taggcagacg cgcccagagc cacggtcatg tagcccagtt gcgacagggt
    27961 ggaatacgca accacacgtt tgatgtcgtt ttgaatcacg cccaagaaac ccataaacag
    28021 agcagtaatc gcaccgatta ccatgatgac agacagcgca gtgctgctca tctcataaat
    28081 cggcgacata cgcgatacca tgaacagacc ggcggtaacc atggttgcgg cgtgaatcaa
    28141 tgcggaaatc ggggtcgggc cttccatcga atcgggcagc cagacatgca gcgggaattg
    28201 tgccgattta cccatcgcac cgacaaacag aagtaaacaa gttacggtaa tcaaagacca
    28261 ttccacgccg gggaaaagct ggatggtggc attttggacg ttgggcagat aggcgaatac
    28321 gtcctgatag cgcaggctgc cgccgaaata ggcaagcacc aagccgatac cgagcaaaaa
    28381 gccgaagtcg ccgacacggt tgatcaaaaa ggctttcagg ttggcaaatg tcgcgctcgg
    28441 gcgtttgaaa tagaaaccga tcaagagata cgacaccaaa cccaccgctt cccaaccgaa
    28501 gaagagctgg atgaagttgt tgctcataat cagcatcaac atgctgaatg taaacaaaga
    28561 aatgtagctg aagaagcgtt ggtagccgac tttttcgtcg tgcatatagc cgatggtgta
    28621 gatatgcacc atcaacgaca cgcccgttac cacgaccatc atcatcgccg tcatcgtatc
    28681 aaccaagaag ccgacggaaa aatccaagcc gcccattgtc agccaggtat agacgttttc
    28741 gtcaaacttg gcacggctgc cattgaggaa tccccacagc acataagccg acagcacggc
    28801 ggacacagcc acgccgagta tcgtaaccgt atgcgcgccg gcacgtccga ttttgttgcc
    28861 gaacaaaccc gcaatcagcg agcctgccaa aggcacgagg gcgatgatga gatataaagt
    28921 catgtcgttc atttaaattt gcaccagtaa aatataaatg ctgattgcga ttaaactaaa
    28981 aatcgtacca tacgcaatca tagaagccga acgccctgtt ttcaaatatg ccgtttccaa
    29041 cacaataata ttggcagcag gcggcagcaa cggtattaaa tacaatgttt tttggttttc
    29101 aagaatcaac ttcaagtcaa aatatgcagc tatttggata aaaagaaaaa caatccctat
    29161 aaataaaatg ttttttatta gaaacggttt aatacttttc ttcaaatcat tcaaatttag
    29221 ttttatctca gccagccaaa taccgagtat caccatcccc aatattccca ttgctatttt
    29281 cgagaaaaaa taaacttccc caaaataatc tgctacaaaa tgccctagcg gaataccgaa
    29341 cattcctatt accaatgcaa caacgggagg ggattttaaa gtctttttca atccggatat
    29401 gaaattacta ttattcaata acaacccgac cccgaccgaa tttccgaata aagaacttcc
    29461 cacgtatgcc gaaataagca cttgcgccgc ctcatctcca aacaaggttg caactatggg
    29521 aagccccaac catccgatgt tcaaatagca caaacacaaa ttccaaacag gatctttatc
    29581 aactcttttc ccgattagga taatcaaccc catcgaaata atcatccata acataacata
    29641 ctgcatacca tcacgatatg tcgttatgtt atagacaata acaaaaggga taatccatct
    29701 gctcaatatt acagaaaaag tgcctttaat ttttctaaaa ggggttttcc caatcaaaat
    29761 gccaataaac agatatacca atggaagagc aattgaaaat gacataatca ctctccactt
    29821 tctgaaattc taaaattttc aaaaacccat tgttttaccc aattacccgc catacagccc
    29881 aatggctgcc aatgagccct tgaaattgta aacattctct ttcactcata ctaatccaca
    29941 atttatgcat atatgggcgt gtggagacca aagtgccttg atatggcaaa caagagtttt
    30001 tacctgtaac tgtttgatta agctaactcc tgctgcacct aatccaacag cagcaatatc
    30061 aaacattatc tatctccttt tttctttaaa aattccaaat caacctttaa gtttgttcaa
    30121 atcggcaacg ttgattgttt gtcggttgcg gtacaccaat accataatcg ccaaaccgat
    30181 ggcagattcg gcagcggcaa cggtcagtac gaagaatacg aaaatctgtc cggcggtatc
    30241 gcccaaatgt tgcgagaagg cgataaagtt gaagttcacc gccaaaagca tcagctcgat
    30301 ggacatcagc aagaccagca cgtttttgcg gttcataaag atgcccatcg cgctgatacc
    30361 gaacaggagc gcgcccaata ccagataatg cgtcaaggta atcatgcttt gccctcccct
    30421 tccggcttga gaccgtctga aacttcgctt tcttcggcag attcgacttg cggtttgacc
    30481 gcttccattt tcaccagacg catacggcct tggtcggcgc gtactttaac ctggtcggca
    30541 ggatccatac gtttcggatt gatggtttta cggtgaacca gcgcaatcgc cgccaccata
    30601 cccaacaaca gcaataccgc cgccaattca aacggcaaca gatagtcggt ataaatacgg
    30661 ctgcccaaat cgcggatatt gttgtaatcg gcaggaatgt ctttcatcag accaaatgcg
    30721 gcaaggtcgg ttttcgggtt gaccagaatc aggatcagcg caaccgccaa caatgtgccg
    30781 accacaccgg caacaggcgc gtgccgccag aaaccggcac gcatttcttc aatgtcgatg
    30841 ttcaacatca tcacgacgaa caggaacaac accatcacgg cgccgacgta aaccaccacc
    30901 agcgtcacgc ccaaaaactc agcctgcatc agcatccaaa gcatcgcgct cacgcagaag
    30961 gtcagcacca gatgcaaagc ggcgtggaca gggtttttag cggtaacggt acgcagcgcg
    31021 ccgtataaga cgatggtggc aaggatatag aacagaatcg cggaaaaagt cattcgtctg
    31081 ctccttaacg gtacggcgcg tcagcggctt tgcgtttggc gatttcggct tcgtatttgt
    31141 cgccgattgc caagaggatc ggcttggtca tgtgcaagtc gccttttttc tcgccgtggt
    31201 attcaaaaat atgcgtttcc acaatcgcat cggttgggca ggcctcttcg cagaagccgc
    31261 agaagatgca cttggtcagg tcgatgtcgt aacgcttggt gcggcgggtg ccgtcttcgc
    31321 gttcttccga ttcgatattg atcgccattg ccggacacac cgcctcgcac aacttacacg
    31381 caatgcagcg ttcctcaccg ttcggatacc gccgctgcgc gtgcaggccg cggaaacgca
    31441 cggattgcgg cgttttctct tcgggaaaat aaatcgtgtc tttgcgggcg aaaaagtttt
    31501 tgagcgttac gcccatacct tttaccaatt cgccaagcag aaaggttttt actaagttag
    31561 ccatattatg ttccctcaaa acagggattt cgttaggtat tcaaaatcgc tttgttcaga
    31621 cggcctcaag atgccgtctg aaacttattt ccacaaattc agcggtgaaa tcatccacac
    31681 gcccaaaacc acgatgtagg cgaagccgat cggaatcagc actttccagc ccaaacgcat
    31741 gatttggtcg taacggtagc gcgggaaggt tgcacggatc cacaggtacc agtacaacac
    31801 cgccgccatt ttcacgaaca tccagaatgc ggaaggtgtg ccgataaagc cccaactttg
    31861 cgggaacggg gacaaccagc cgccgaggaa catcagcgat gtcagcgcgg caatcagaat
    31921 catgaaaatg tattcggcaa ggaagaacag cgcgaacgcg aagccggaat attcgacgtg
    31981 gtgaccggca acgatttcag actcaccctc tgccacgtca aacggcgcac ggttggtttc
    32041 ggcaacggcg gaaatcagat aaacgatgaa gatggggaag agcggcagcc agttccacga
    32101 gaacacggaa ccgcccgcga tgcctttggc ttgtgcggca acgatgtcgg agaagttcat
    32161 gctgcccgat accatcacaa cgcacaccag cgcggcactc atggcaatct cgtaggaaat
    32221 gctttgtgcg gaagcacgca ttgcacccaa gaacgaatat ttggagttgg aagcccagcc
    32281 cgcgatgatc acgccgtaaa ccgacagcga ggtaatcatc aggatgtaca aaaggccgat
    32341 attgatgttg gtcagcaccc attcttcgtt gaacggaatc actgcccatg ctgcgaaaga
    32401 cggagcaagc gacataatcg ggccgatgta gaacagggct ttgtttgaca gcttcggacg
    32461 ggttacctct ttaaacaaga gtttgaacac gtcggcaaac ggctgaatca gaccccacgg
    32521 gccggttacg ttcggaccga cgcgaagctg catgaagccg atgactttac gttcgaaata
    32581 cgtcaggtag gcaacggtca gaatcagcgg aatcaggata atcacgattt tgacgatgac
    32641 ggataccacc aagcctacgg tgatgcccaa atcgcccaga ccgagcgttg cggcaaagag
    32701 gttttggaac cattcttgca tgatcaagct cccgccagtt caatagtgtc catcaacgca
    32761 cccagcgcgg cattttcggt atgcagcggc agatgcacca cgttttcagg caaaccggca
    32821 tcggctttga cagcaaccga tacgcttgcg ccgttttgtt tggcgacagc ggtttgtccg
    32881 tcttgcaggc ccaagcgtgc caatgtgttc ggatttacac gcgcggcagg cacggcggca
    32941 tggctggttt cttgcaacgg tgcggaacgg cgtacgatgg catcggtgtg ataaataccg
    33001 acgccgccga cacggacgag gcggtctgat gccgtctgaa cgccctcccc tgtccatgta
    33061 ctgcggttgt ccagtttgga cgacaggctt tctgcatcca gcgcgtcttt caaaatcgca
    33121 gcggtatcgt ggtattcaaa acctttcagg tcaaacagat tgcccaatac gcgcaacact
    33181 ttccacatcg gacgcgaatc gccgaagcct tgtaccacgc cgtggaaaga ttgcagacgg
    33241 ccttccatat tgatgaagct gcctgaggtt tcggtaaacg gcgcaatcgg caacaacacg
    33301 tcgcacacgt ccagcagcgt ttcgctgaca aacggtgtaa acgccatcac gctttttgcc
    33361 tgtttcaacg cggctacggc ttttgcaccg tcaaccgtat caatttcagg ctcgacgttg
    33421 agcagcaaga ctgcctgttt cggcgcgttt gccatttcgg taacgctctc acctgagttc
    33481 acgcccaata cgtccgcacc gacgctgttg gcggcttgcg gcaaaatacc cagtaccgcg
    33541 ccggttgcat ccgccagctc ttgcgcggcg gcataaatgg cggcgtaatc aggatggttt
    33601 tgcacttccg cgcccaaaat caccgctgct ttttcagcgt ttttcaggct ggcggtaacg
    33661 gcgtgttccg cattggcaga caggtttttc agacggcctg cccactcgtc gggatgtgcg
    33721 gcttcttgag agagaagcgg catcaacaac tcttctttac tgccggccaa tacactcaac
    33781 gccatacggt ctttggcggc gcggcgcagg cgggcggtca ggagcggctg ttctttgcgc
    33841 aggttcgcac cgactaccaa tacggcatcg ttgtccgcca gcgattcaat gctttgtccc
    33901 aaccattgcg cacctttaag gccgtctgaa agacgtttgt cttgttggcg caaacgggtg
    33961 gcaaagtttt taacacccaa gccgtcggca agttttttgg ccagatacag ctcttcaacc
    34021 gtattcatcg ggttcgccca aatgccgact tggttttggt tgccgtcttt ggcgatacat
    34081 tcaatcgcgc tgcggacata ttccaacgcg gttttccaat ccacgtccat ccactcgccg
    34141 ccctgtttga ttttcggttt tttcagacgg ctttcgtgat acaggccttc gtaggaaaaa
    34201 cggtcgcggt cagacagcca acattcgtta atcgcttcat tttccagcgg caacacgcgg
    34261 cggacggtat ggtctttggt ttgtacaatc aggttgctgc ccaaagcgtc gtgggcggaa
    34321 acggatttgc ggcggttcaa ttcccaagta cgcgcattga agcggaacgg tttgctggtc
    34381 agcgcgccga cgggacacaa atcaatgacg ttgcccgaca attcggtttc caccgttttg
    34441 ccgataaagg gcatgatttc ggagtgttcg ccgcgattca ccatcgcaat ttcctgcaaa
    34501 ccggcgattt cttcagtgaa acgaacgcag cgggtgcagt ggatacagcg gctcatttcc
    34561 tcggcggaaa ccaaggaccc catatctttg ccgacgacgg aacgtttttc ttcggtgtag
    34621 cggctggtgg tttttccgta gcccaccgcc aaatcctgca actggcattc cccgccttgg
    34681 tcgcaggtcg gacaatcaag cggatggttg atgagcagga actccatcac gccttcctgc
    34741 gcctctcggg cttttgccga atgcgtacgc acaatcatgc cgtctgtaac cggcgtggca
    34801 caggcaggca ggggttttgg ggctttttct acgttcacca gacacatacg gcagttggcg
    34861 gcgatggaaa gttttttgtg gtaacagaaa tgcggaatat aagtaccgag cttgtgcgcg
    34921 gcttcaatca ccgtcgcgcc ctgctccaca gatacttgtt tgccgtcgat ttcgatttgt
    34981 aacatggttc gttcctagtt acgggtattt gataaataat cttttatgga ggtcgtctga
    35041 aaaatgggtt tcagacggcc ttttgagttt cattgacaat caatactgtt taaaacgttt
    35101 actgccacct tgccgtcatt cccgcacagg cgggaatcca ttttttgaat ttcggcaact
    35161 gcttttcaaa tatcgggttc tgtaaattcc actatggatt cccgcctacg cgggaatgac
    35221 ggcaaagtta aatttttagc attttgcttt taaccaatat aaaaccaact aaaactataa
    35281 ctcatttatt atatttttaa atgacttaaa taactcttta acagcacgct cattgtcttc
    35341 atctgtaggc tcaaactttt cacaccaatt aattatattt acatcgccat gcaataactc
    35401 tgcataacat cctactgcat cgcaactacc accccgtgtc cttgacgtgc caaaaataaa
    35461 atcacaatta tgcgaataaa gtttgtataa atttctccta acctcgtatt cattatcacc
    35521 agatgaataa aaaccaattc tctgaccttc atgatcaaat actgcaacaa aatctaagcc
    35581 atttccatgt ctttcaaaaa agactagaaa tttagaaaat ttccaacaaa tctgattaaa
    35641 aagcgtattg agtgtcgtac tcttaccttt gttggctgca ccgtatagaa taaaaatttt
    35701 agcttccacc attaccaccc tccccactta tgctctttca tcggtccgcc gtgttcgatg
    35761 taatgcacaa actcatcacg gaaatgcttg gtaaagcttc ggacggggaa gacggcggca
    35821 tcggcaaggg cgcagatggt gcggcctgcc atttggttgc cgacagaatc cagcaaatcc
    35881 aaatcttcca tgcggccttt gccttctacg atgcggtgga cgatgcggta aagccagccg
    35941 gtgccttctc ggcaaggcgt acattggccg caagactcgt cgtagtagaa gtagctcagg
    36001 cgctcaaggg ctttcaccat gcacacgtct tcgtccatga cgataatcgc gccagaaccc
    36061 agcatcgagc ctgctttgga aatcgagtcg tagtccatat ttgtctgcat catgatgtcg
    36121 gcaggcaata cgggcgcgga cgaaccgcca ggaataacgg ccttgagttt tttgccgccg
    36181 cgcataccgc ctgccatttt caagacttcg gcaaacggtg tgcccaatgg cacttcatag
    36241 ttgcccggac gctcgacatg gccggaaata cagaataatt tggtaccgcc tgcattcgga
    36301 atacctttat cggcaaatgc ctgtccgccg tcacggataa tgaatgaaac ggaggagaaa
    36361 gtttcggtat tgttgatggt agtcggtttg ccgtacaggc cgaacgaagc agggaatggc
    36421 ggtttaaagc gcggctggcc ttttttgcct tccagcgatt caagcaatgc ggtttcttcg
    36481 ccgcagatgt atgcaccgta gccgtggtgg gcgaagagtt cgaattcaaa atccgaaccc
    36541 aaaatatttt tacccaaaaa gcctgcggca cgcgcctgct ccaaagcggc ctcaaagcgt
    36601 tggtagcctt caaaaatttc gccgtggata tagttgtaac cggctttcgc gcccatcgcg
    36661 taaccggcga taatcatgcc ttcaatcagg gcatgggggt tgaacataat aatgtcgcgg
    36721 tctttaaatg tacccggctc gccttcgtcg gtgttgcaaa cgacgtactt ttcgccgggg
    36781 aaagaacggg gcataaagct ccatttcaaa ccggtcggga agcccgcacc gccacgaccg
    36841 cgcaagccgg aggttttgac ttcgtcaatc acatcggttt gcgagatgtt ttcggacagg
    36901 attttacgca gggcggtata accgccacgt ttgacgtatt cgtccaatgt ccagcaatcg
    36961 ggattggcgg tatccacttg gtcaaaaatc acgcctgatt ggtaaatagc catttttggt
    37021 gtgcctgttt gttttcgtat cggttgcggc cgctgtttca gacgacctta agatgtcttt
    37081 gtgtaccggc ttgtaacgtc gtctgaaata aaatctagtt tatcaaatcg atattttcag
    37141 acgaccttta atcgtttttg ttggatctc
//
LOCUS       DAXIHS010000009        36294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXIHS010000009 DAXIHS010000000
VERSION     DAXIHS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.009"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(281..1723)
                     /gene="aldA"
                     /locus_tag="ACFOF2_000367"
     CDS             complement(281..1723)
                     /gene="aldA"
                     /locus_tag="ACFOF2_000367"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC7106748.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1758..1958)
                     /locus_tag="ACFOF2_000368"
     CDS             complement(1758..1958)
                     /locus_tag="ACFOF2_000368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106749.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1967..2356)
                     /locus_tag="ACFOF2_000369"
     CDS             complement(1967..2356)
                     /locus_tag="ACFOF2_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106750.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2565..3482)
                     /locus_tag="ACFOF2_000370"
     CDS             complement(2565..3482)
                     /locus_tag="ACFOF2_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC7106751.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYMEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3655..4455
                     /locus_tag="ACFOF2_000371"
     CDS             3655..4455
                     /locus_tag="ACFOF2_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC7106752.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4503..5279
                     /gene="mlaE"
                     /locus_tag="ACFOF2_000372"
     CDS             4503..5279
                     /gene="mlaE"
                     /locus_tag="ACFOF2_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC7106753.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5330..5824
                     /gene="mlaD"
                     /locus_tag="ACFOF2_000373"
     CDS             5330..5824
                     /gene="mlaD"
                     /locus_tag="ACFOF2_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC7106754.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5861..6451
                     /locus_tag="ACFOF2_000374"
     CDS             5861..6451
                     /locus_tag="ACFOF2_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC7106755.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6502..6780
                     /locus_tag="ACFOF2_000375"
     CDS             6502..6780
                     /locus_tag="ACFOF2_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC7106756.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6777..7604
                     /locus_tag="ACFOF2_000376"
     CDS             6777..7604
                     /locus_tag="ACFOF2_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC7106757.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7729..8091
                     /locus_tag="ACFOF2_000377"
     CDS             7729..8091
                     /locus_tag="ACFOF2_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC7106758.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8091..8474
                     /locus_tag="ACFOF2_000378"
     CDS             8091..8474
                     /locus_tag="ACFOF2_000378"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC7106759.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8471..8959
                     /locus_tag="ACFOF2_000379"
     CDS             8471..8959
                     /locus_tag="ACFOF2_000379"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC7106760.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(9042..9170)
                     /locus_tag="ACFOF2_000380"
     CDS             complement(9042..9170)
                     /locus_tag="ACFOF2_000380"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC7106761.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9176..9556)
                     /locus_tag="ACFOF2_000381"
     CDS             complement(9176..9556)
                     /locus_tag="ACFOF2_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC7106762.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9794..10009
                     /gene="rpmE"
                     /locus_tag="ACFOF2_000382"
     CDS             9794..10009
                     /gene="rpmE"
                     /locus_tag="ACFOF2_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC7106763.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            10325..10954
                     /locus_tag="ACFOF2_000383"
     CDS             10325..10954
                     /locus_tag="ACFOF2_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC7106764.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            11100..12761
                     /locus_tag="ACFOF2_000384"
     CDS             11100..12761
                     /locus_tag="ACFOF2_000384"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC7106765.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
     gene            12878..13483
                     /locus_tag="ACFOF2_000385"
     CDS             12878..13483
                     /locus_tag="ACFOF2_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC7106766.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            13555..13947
                     /locus_tag="ACFOF2_000386"
     CDS             13555..13947
                     /locus_tag="ACFOF2_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC7106767.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(13976..14125)
                     /locus_tag="ACFOF2_000387"
     CDS             complement(13976..14125)
                     /locus_tag="ACFOF2_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106768.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFNTPKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(14146..14592)
                     /locus_tag="ACFOF2_000388"
     CDS             complement(14146..14592)
                     /locus_tag="ACFOF2_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC7106769.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSVKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(14724..14936)
                     /gene="rpsU"
                     /locus_tag="ACFOF2_000389"
     CDS             complement(14724..14936)
                     /gene="rpsU"
                     /locus_tag="ACFOF2_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC7106770.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(15225..17072)
                     /gene="ltgA"
                     /locus_tag="ACFOF2_000390"
     CDS             complement(15225..17072)
                     /gene="ltgA"
                     /locus_tag="ACFOF2_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC7106771.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAVATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSHVGAQGLMQVMPATAREIA
                     GKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            17603..18340
                     /locus_tag="ACFOF2_000391"
     CDS             17603..18340
                     /locus_tag="ACFOF2_000391"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC7106772.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKTRVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            18342..19028
                     /locus_tag="ACFOF2_000392"
     CDS             18342..19028
                     /locus_tag="ACFOF2_000392"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC7106773.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKAVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIIRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            19187..20050
                     /locus_tag="ACFOF2_000393"
     CDS             19187..20050
                     /locus_tag="ACFOF2_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC7106774.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPVAWNEGAAK"
     gene            complement(20112..20681)
                     /locus_tag="ACFOF2_000394"
     CDS             complement(20112..20681)
                     /locus_tag="ACFOF2_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC7106775.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            20839..21699
                     /locus_tag="ACFOF2_000395"
     CDS             20839..21699
                     /locus_tag="ACFOF2_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC7106776.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            21835..22173
                     /locus_tag="ACFOF2_000396"
     CDS             21835..22173
                     /locus_tag="ACFOF2_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106777.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            22541..22894
                     /locus_tag="ACFOF2_000397"
     CDS             22541..22894
                     /locus_tag="ACFOF2_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC7106778.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            22884..23750
                     /gene="atpB"
                     /locus_tag="ACFOF2_000398"
     CDS             22884..23750
                     /gene="atpB"
                     /locus_tag="ACFOF2_000398"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC7106779.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            23807..24043
                     /gene="atpE"
                     /locus_tag="ACFOF2_000399"
     CDS             23807..24043
                     /gene="atpE"
                     /locus_tag="ACFOF2_000399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC7106780.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            24114..24584
                     /locus_tag="ACFOF2_000400"
     CDS             24114..24584
                     /locus_tag="ACFOF2_000400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC7106781.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            24589..25122
                     /locus_tag="ACFOF2_000401"
     CDS             24589..25122
                     /locus_tag="ACFOF2_000401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC7106782.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            25133..26680
                     /gene="atpA"
                     /locus_tag="ACFOF2_000402"
     CDS             25133..26680
                     /gene="atpA"
                     /locus_tag="ACFOF2_000402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC7106783.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVVSVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            26705..27580
                     /gene="atpG"
                     /locus_tag="ACFOF2_000403"
     CDS             26705..27580
                     /gene="atpG"
                     /locus_tag="ACFOF2_000403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC7106784.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            27618..29015
                     /gene="atpD"
                     /locus_tag="ACFOF2_000404"
     CDS             27618..29015
                     /gene="atpD"
                     /locus_tag="ACFOF2_000404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC7106785.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            29026..29448
                     /locus_tag="ACFOF2_000405"
     CDS             29026..29448
                     /locus_tag="ACFOF2_000405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC7106786.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            29853..30758
                     /gene="glyQ"
                     /locus_tag="ACFOF2_000406"
     CDS             29853..30758
                     /gene="glyQ"
                     /locus_tag="ACFOF2_000406"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC7106787.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            30835..31245
                     /locus_tag="ACFOF2_000407"
     CDS             30835..31245
                     /locus_tag="ACFOF2_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC7106788.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLEVNDFGMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHTTPLVDGFYGDDGWENYSETYKQAD"
     gene            31266..33329
                     /gene="glyS"
                     /locus_tag="ACFOF2_000408"
     CDS             31266..33329
                     /gene="glyS"
                     /locus_tag="ACFOF2_000408"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC7106789.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            33346..34392
                     /locus_tag="ACFOF2_000409"
     CDS             33346..34392
                     /locus_tag="ACFOF2_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC7106790.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     GGGYIARTDADDIASPGWIEKIVGEMEKDRSIIAMGAWLEILSEEKDGNRLARHHKHG
                     KIWKKPTRHEDIAAVFPFGNPIHNNTMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQTKAAAYELPEKDLPEEDFERARRFLYRCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            34434..35261
                     /locus_tag="ACFOF2_000410"
     CDS             34434..35261
                     /locus_tag="ACFOF2_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC7106791.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            35273..35368
                     /locus_tag="ACFOF2_000411"
                     /pseudo
     CDS             35273..35368
                     /locus_tag="ACFOF2_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            35410..36252
                     /locus_tag="ACFOF2_000412"
     CDS             35410..36252
                     /locus_tag="ACFOF2_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC7106792.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         8591 a         9631 c         9382 g         8690 t
ORIGIN      
        1 tgtaaatctt agagatggcg gaatatagcg gattaacaaa aaccagtacg gcgttgcctc
       61 gccttagctc aaagagaacg attctctaag gtgctgaagc accgagtgaa tcggttccgt
      121 actatctgta ctgtctgcgg cttcgtttaa atttaatccg ctatacaaag acaaaaacat
      181 cgacctcggt catgactgat tgccgatgaa gcaataaaaa tgccgtctga acctgaaaac
      241 gggttcagac ggcattttct atcggggttt taaagcggca ttaaatgtcg gtttccaaat
      301 aaacgacttg ggtttgcaga tattcttcca aaccgtgttt gccgtccgcg ccgccgatac
      361 cggatttttt ccaaccggcg tggaaaccct gcatcgcttc aaagttttcg cggttgatgt
      421 aggtttcgcc gaattgcagg cggcgggtaa cgtagaaggc ttcgtttaaa ttagtcgtat
      481 aaacagaact ggtcagacca aactcgcaat cgttagccag tgcgatgacc tggtcgagcg
      541 tgtcgaaagc ggaaacgggc agcacggggc cgaaggtttc ttctttcata atgtccatac
      601 tgttgtcggt gtcggtcagc agggtcggct cgaagaaata accgcgtcct tcggcgcgtt
      661 tgccgccgca aaccaatttc gcaccttgtt tgactgcccg ttccactttt tcggcaacgg
      721 ctttgacggc gcgctcttca atcagcgggc ccatttccag cgcgcctgct tcggcttcgg
      781 cagggttgcc gtagcgtacg cctttcatgg cggcggtcat tttttcaatg aatgcgtctt
      841 tcagactgct gtggacataa acgcgctcgg cgcagttgca gatttgacca gtgttgccga
      901 cgcgcgaagc caagatggat ttcactgcca agtccaaatc cgcgtctttc aaaacgatag
      961 caggtgcttt gccgccgagt tccagcgaga ctttggtgat gttggcagaa gcggcttcca
     1021 tcacttggcg gcccgcttca acggagccgg tcaggctgac catatcgact tgcggatggg
     1081 cggacaaggc attgccgatt tccgcacccg gaccgttcac aacgttgaac acgcctgctg
     1141 gcagtccgac cgcatcgacg atttcggcga agatgtggca gttgatcggg gttacgctgc
     1201 tgggtttgac gacgatggtg ttgcccgtta ccaaagcggg gcccattttg cgggcaatca
     1261 ggaagaaggg gaagttccac ggcaaaatgc cggcgattac gcccagcgga cgtttgaaca
     1321 ataaaatatt ttcgcgcggg cggtcgcttt ggatgatttc gccttcgtaa cgacgcgccc
     1381 attcggcttg gtaatcgaga tagtcggcgg taaacatgac ttccacgcgc gccaagtctt
     1441 tggttttgcc gccttcggca acgatggtgt cggtcagctc gtcggcgcgt ccgcgtatgc
     1501 cttgggcgat tttgcgcaaa tacgcgccgc gttcgaccgc aggcaggcgt tcccaagccg
     1561 gttgcgccgc gcgtgccgcc gccacagctc ggtcaacgtc tgcttttccg cctttgggtt
     1621 cgcgggcgat ggtttcttcg gtggatgggt tcaatacgtc gcgccattcg ccgttgaaat
     1681 cgttttcaaa gcgtccgttg atgtacatgg ccaattgttt catttcgggt tctccagttt
     1741 tgtagtcaga tgtagtctta gtttattccc aaataaattg ccgtacaagt ttcttttgaa
     1801 atgtttagac gagaagctac tatgtctgca tattgttctg ctaatttata ttcactcgaa
     1861 tgcaactgcc gattattcca atctgcattc gcatcagtaa aagccccagc attgccgcct
     1921 gtggcataac caatcgctaa actgccaacg gtatccacaa caggtttcag gctgcctgaa
     1981 tttctctaat aaatgaaaca cttattctat cagccgttaa tgtaaccaaa caattatcaa
     2041 gataagagta gtttactaaa tactcatctt cttcaaattg tatattttgt agggttagat
     2101 gattattttt taattctgcc gttatttcat acttgaaact atcatctatt tttctaaatc
     2161 caatatttga agttgattta tccagtatgt aatcattaaa gatttcatag gataattcca
     2221 cttcataaaa ttgattttca aatattttga atgggcagta tgatataaaa caatgtagtt
     2281 cataaccatt tatttcaatc aatactgcct cttcaatggt gttatctaac tttttgattt
     2341 tacatttata taacatttta tcttgccttt agataaattg ttttgttttt tggattgtta
     2401 ttcaaagttc tccaaaagtc ttttcctgat tttgtatgta tcgtggtaac actaccattt
     2461 tcattggttc caacaaaaat aaatttagat tgacctttgg aatcagtacc caaaaactga
     2521 acatatccag acatgcggat ttcgggtttt aaatcaaatg cggcttaccg gttttctttt
     2581 ttggtttccg caggcgtgct gccgaaatat tgcctgaatg cctgagtaaa gctggaaacg
     2641 tggcggtagc cgcacaaata cgcggtttcg cctatgcttt tgcccccgtt ttgcaacagg
     2701 tagagcgcgt gctgcatttg tttgtggtgc agccactcgc ttgcggtaat gccgaaatgg
     2761 tcgcgcatac ggcgttgcag cgtcctttcg ctgatgttca gcgcgtctgc cagccggttg
     2821 acttggtgtg cgccgtcggc aaacgcggca tttagggtgc ggctgaagtc ttcagacggc
     2881 atagcgtctg cttccgccgt ttgccccgcc gccggcccga tgccgtctga aaccgtgtcc
     2941 cacaaatccg acagcagccg caaaacgtcc gcctcgcggc gcaatgtttc gcccaaatgc
     3001 ccctttggga cggcttgcag gcaggatgcc gccaaaccgc gcaggtttgg ggacaaattc
     3061 catattctga ccggttcgcg gtaaagtatg ggtgtaaaac acgcatactc cggacgcagc
     3121 agccattgct ccataccttt aattgtcatt ttgaccgttt tgccgcctcg ataaagatag
     3181 cggctaaaca ggacttcttc cccgactgaa atcaggatga ccttgccgcc gtccgcatcg
     3241 atttggaatc ggctccggtt gatgccgaag ttcaaagagc cttccagcaa aagcacgaac
     3301 gacacataag gttcggcgag gcggctgctg caaaaatcgc accgtgccgt taccgtgccg
     3361 ccgtggatgg aaatgccgtt ggacaaagta tcgaaacggt agctgccctc catatagctg
     3421 ccgccgccgc ttttgaccag attgacgaac cggctgctgt tcaaatgttc tgctttttcc
     3481 atagttttct gataaaggtt atcatttgaa agataacatt tttcgccaca gcaaacaatc
     3541 cgccgccgac aacggcattt tcacaacact taccgtccgt atgccgtctg aaaattgccc
     3601 gtcttcccga taagccttaa aatacacgcc gtcaaaccta ccgtccgtcc gtacatgagc
     3661 ccatccccct ttatcgaaat gaaagacgtc gccttcgcgt atggcgaccg cccgattctg
     3721 aacgacatca atttcagtat tccgcaaggt aattttgccg ccgtgatggg cggttcgggc
     3781 agcggcaaaa ccacgctgat gcgactgatt acaggacaga ttcgtccgca gtccgggcag
     3841 gttttgattg aaggacgtga tttgtcgggc ttttcggctg acgagttgta tgaacaccgc
     3901 cgccgtatgg gcgtattgtt ccaacacggc gcgctgttta ccgatttgtc tgtatttgac
     3961 aatattgctt ttccgatgcg cgaactgacg caactgccgg aagcggtgat tcgagatttg
     4021 gttttgttga aactgaacgc ggtcggtctg cgcggtgtag aaaacctgat gccgtccgag
     4081 ttgtccggcg ggatgtcgcg ccgcgtcgcg cttgcccgca cgattgcgct cgaccccgaa
     4141 attatgttgt acgacgaacc gtttaccggt ctcgatccga tttccttggg cgtgattgcc
     4201 cacttgatca gccgcgtcaa caaggctttg cgttcgacca gtattatggt cacgcacgac
     4261 attgaaaaat ctttggaaat cgtcgatcag gtgattttct tggcgcacgg cgaaattatg
     4321 ttctccggct cgccgcagga aatgcgcgaa ctggattcgc cttgggtgcg ccagtttgtc
     4381 ggcggcttgg cagacggccc cgtggcatac cgttatccgg cgcaaacgtc gttgcagcag
     4441 gatttgctcg ggtaaatgag ccggtgccgt ctgaaaccgt tgaactttac atatagaaat
     4501 ttatgaactt tatccgttcc gtcggggcga aaaccctcgg ccttattcaa tccctcggca
     4561 gtattacgct gtttctgctg aacatcttgg cgaaatccgg tacggctttc gtccgtccgc
     4621 gcctgagcgt gcgccaagtg tatttcgccg gcgtgctgtc ggtgctgatt gttgccgttt
     4681 cggggctgtt cgtcggtatg gttttgggtt tgcagggtta tacgcagttg tcgaaattca
     4741 aatccgccga tattttgggc tatatggtcg cggcttcgct attgcgcgag ctgggtccgg
     4801 tattggcagc gattttgttt gccagcagcg cgggcggtgc gatgaccagc gaaatcggtc
     4861 tgatgaaaac gaccgaacag ctcgaagcga tgaatgtgat ggcggtaaac cctgtcgccc
     4921 gcgtggttgc gccgcgcttt tgggcgggcg tgttttccat gccgcttttg gcttcgattt
     4981 tcaacgtggc gggcattttc ggtgcgtatt tggtcggtgt aagctggctg ggcttggaca
     5041 gcggcatttt ctggtcgcaa atgcagaaca acatcacgat acattacgat gtaatcaacg
     5101 gtctgattaa atccgccgcg ttcggcgtgg cggtaacgct gattgccgtg catcagggct
     5161 tccattgcgt cccgacttcg gaaggcattt tgcgcgccag cacgcgcacg gtggtttcgt
     5221 ccgccctgac gattttggcg gtcgatttta tattgaccgc gtggatgttt acagattgac
     5281 aggccgtctg aagacgaaac gcggaacatc ggatattcaa ggaactttaa tgaaaaagaa
     5341 catattggaa ttttgggtcg gactgtttgt cctgctcggc gtggcagcgg tcggctttct
     5401 cgctttccgc gtggcaggcg gcgcggcctt cggcaattcc ggcaagactt ataccgtata
     5461 tgccgatttc agcgacatcg gcggactgaa ggccaacgcg ccgatcaaat ccgcaggcgt
     5521 attggtcgga cgcgttggtt ccatcgagct tgacccgaaa tcctatcagg cgaaagtccg
     5581 tctcgatttg gacagcaaat atcagttcag tagcgacgtt tccgcacaaa tcctgacttc
     5641 gggacttttg ggcgagcagt acatcgggct gcagcagggc ggcgacacgg aaaaccttgc
     5701 tgccggcgac accatctccg taaccagttc tgcaatggtt ctggaaaacc ttatcggcaa
     5761 attcatgacg agttttgccg agaaaaatgc cgacggcggc aatgcggaaa aagccgccga
     5821 ataaacgtat gccatcatat tcaaactagg acacttaatc atgaaaaaat cctccctcat
     5881 cagcgcattg ggcatcggta ttttgagcat cggcatggca tttgccgccc ctgccgacgc
     5941 ggtaagccaa atccgtcaaa acgccactca agtattgagc atcttaaaaa acggcgatgc
     6001 caacaccgcc cgccaaaaag ccgaagccta tgcgattccc tatttcgatt tccaacgtat
     6061 gaccgcattg gcggtcggca acccttggcg caccgcgtcc gacacgcaaa aacaagcgtt
     6121 ggccaaagaa tttcaaaccc tgctgatccg cacctattcc ggcacgatgc tgaaattaaa
     6181 aaacgccaac gtcaacgtca aagacaatcc catcgtcaat aaaggcggca aagaaatcat
     6241 cgtccgcgcc gaagtcggcg tacccgggca aaaacccgtc aacatggact tcaccaccta
     6301 ccaaagcggc ggtaaatacc gtacctacaa cgtcgccatc gaaggcgcga gcctggttac
     6361 cgtgtaccgc aaccaattcg gcgaaattat caaagcgaaa ggcgtggacg gactgattgc
     6421 cgagttgaaa gccaaaaacg gcggcaaata accgacaaac cataccgtcc gagggcggta
     6481 tgggttttcc gagcaaacgc tatgcataca gaactcaaaa acggaatatt gcacatcggc
     6541 ggcgacatca ccgtcaaaac cctgaccgcc gccgctttta cccgcttcag acagcaatgt
     6601 cgtctgaaag acacctgcgc ggttgatttg agcggcgtcg gacgcgccga ttccgcctgc
     6661 gtgtcgctgc tgctcgaagt gctgcgcggt tgcaaaggca gcgtcaggct gaccggcatt
     6721 cccgaatccg tgcgcgcgct gtccgaactg tacgaaatca aagactggct gaaatcatga
     6781 aaaaaaccgc ctatgccttc ctcctgctga tcgggttcgc ttccgcccct gcatttgccg
     6841 aaacccgccc cgccgaccct tatgaaggct acaaccgcgc cgttttcaaa ttcaacgacc
     6901 aagccgaccg ctacattttc gcccctgccg cgcgcggcta ccgcaaagtt gcgccgaaac
     6961 ccgtccgcgc cggcgtgtcc aattttttta acaacctgtg cgacgtggtc agcttcggta
     7021 gcaatatctt gcgcttggac atcaaacgcg caagcgaaga ccttgtccgc gtcggcatca
     7081 acaccacttt cggtttgggc gggcttatcg acatcgccgg cgcgggcggc attcccgaca
     7141 ataaaaacac cttgggcgac acgtttgctt cgtggggatg gaaaaacagc aattatttcg
     7201 tgttgcccgt cttagggccg tccaccgtcc gcgacgcgct cggcacgggt attacctccg
     7261 tttattcgcc caagaatatc gtcttccgca cccctgtcgg acgctggggc acgactgccg
     7321 tatccgccgt cagtacgcgc gaaggtctgc tcgatttgac cgacagtctg gacgaagccg
     7381 ccatcgacaa atacagctac acgcgcgacc tctatatgaa agtccgtgcg cggcagaccg
     7441 gtgcaacacc tgccgaaggt acggaagata acatcgacat cgacgaattg gtcgaaagtg
     7501 ccgaaaccgg cgcggcggaa actgccgttc aagaagattc cgtatccgaa acacaggcag
     7561 aagcagcagg ggaagccgaa acgcaacctg gaacacaacc ttaaagccat gccgtctgaa
     7621 aatccgtgtt cggacggcat tcccttctgt cggaacggac agtccgaaac cacagattgg
     7681 gctataatct cttttttgcg cataccgcgc gacagcaaag gaaaaaccat gtacgaagtc
     7741 aaccgcagcg tattcgtcct catcccttta gaaccttttt ggaactggct gcaaaccctg
     7801 ccgggcaacc acctcgacgg actgactctt gaagacatcc aagccgacgc caattcctac
     7861 cttgtccgcc cgtgcgaaac cgccgacgaa gtatgggacg aaatcgaagc ccgttttgaa
     7921 gacattttcg ccgccgaact tgccgactgg tgcgaagacg aacgcgagtg gcctgcgctc
     7981 gatgccgaca ttttcaacga atggttcgac atccagcttt ctaccgtcat caccgacctc
     8041 gaacacgaac cgcttgcccg cgaagccttc caacccatca acctgaactg atgaagctga
     8101 ccgtccgcaa ctaccatctc gacggctacg gccatgtcaa caatgcgcgc tacctcgaat
     8161 ttttcgaaga agcgcgctgg gcgttttttg aagaacgcgg actgctgcac gagttggcag
     8221 gcctcatact gattgtcgcc cgcatcgaca tccgatacag ccgccccgcc gtcgaaggcg
     8281 acgtattgca gttttcatgc cgcctgaaaa cccccggcac gcgccgtatt gtgctgaccc
     8341 agacgattac actgccaaac ggcaaaaccg ccgcagaagc cgacatcacc ctgatgcccg
     8401 tccacgccgc cacacaacgc accgtcagcc tgcccgccac ccttgcccgc gcattggaag
     8461 ccttgtccga atgaaaaaaa tactcaccgc cgccgtcgtc gcactgatcg gcatcctcct
     8521 tgccatcgtc ctcatccccg acagcaaaac cgcgcccgcc ttctccctgc ccgacctgca
     8581 cggaaaaacc gtttccaacg ccgacctgca aggcaaagta accctgatta atttttggtt
     8641 tccctcctgt ccgggttgtg tgagcgaaat gcccaaaatc attaaaacgg caaatgacta
     8701 taaaaacaaa aacttccaag tccttgccgt tgcacaacct atcgatccga tagaaagcgt
     8761 ccgccaatat gtcaaagact acggtttgcc gtttaccgtc atgtatgatg cggacaaagc
     8821 tgtcggacag gcgttcggca cacaggttta tccgacttct gtcctcatcg gcaaaaaagg
     8881 cgaaatcctc aaaacttatg tcggcgaacc cgatttcggc aaactctacc aagaaatcga
     8941 taccgcgctg gcacaatagc gatgccgtct gaagcgcaaa aaaaacagaa acgatgttcc
     9001 tgttttttca gatcatccgc cccgacacgt tcagacggca ttcaaccttc cggcagattc
     9061 tttttcacca ctgccggatt gcctgccgcc aaagagtagg gcggaatgtc ttttgtaacc
     9121 accgcgcctg cgcccacgac cgaaccgcgt ccgacggtta cgcccgccat tacaatcacc
     9181 ctgtgccccg gccagacatc gtcctccaac gtaatcggac ggatttccgt gtagccctca
     9241 aagcgttttt tgaacggtca aacttgtggt tatttgaata aaacagacat tccggctcca
     9301 tcatcacatt tttgccgacc accagcccac ggcagatttc acagtttgcc ccgatgcccg
     9361 agccgtcgcc caaaaccgta tccggaaaca catacgcccc gcgttcgata ttgaccccgc
     9421 gtccgatatg cggagaaacc cgccgcgcca aaaaaccgcg cacccgtctg ccgacgatgc
     9481 cgatgccgcg catatgtgac ggcggcaacc ccgaaccggc aaaacgcagc agcaggaaaa
     9541 agaattttga tcccatcaaa cctccctcaa aagaactttt tatagtggat taacaaaaat
     9601 caggacaagg cgacgaagcc gcagacagta cagatagtac ggcaaggcga ggcaacgctg
     9661 tactggtttt tgttaatcca ctatacattt tatcgaatat cctcttgacg aaacaacaga
     9721 aaatagcgag aatgccgagc ttgtctgaag cataggtaca tcccctatgc ccgctttttt
     9781 gaaaggaaat caaatgaaac aaggtattca cccgaactac cacgaagtca acgttacctg
     9841 ctcttgcggc aacaaattcg caaccaaatc cgcgatggaa aaagaaaact tcaatatcga
     9901 ggtttgctcc ctgtgccacc cgttctatac cggcacccaa aaaatcgtcg ataccaccgg
     9961 ccgcgtggac aaattcaaca acaaattcgg caacctgttc aaacgctgat tgccgctttg
    10021 caagaattaa gccctgccat catttcgatg gcagggcttt ttaatatttg gcacactgac
    10081 ttcttatcta tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    10141 caaatagtac ggaatcgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
    10201 gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatattttg atggggttga
    10261 agttgacatc tacaaagttt aatgtttgaa tacattaaaa aaatatattt gaatgagatg
    10321 ttttatgatt caaaatgttg ttacttcaat aatcctgtat tctgggacag ccgtagactt
    10381 acttattatc ctaatgttat tttttgccaa aagaaaaagc agaaaagaca tcattaacat
    10441 ctatttagga caatttctag gctctgttag cctgatattg ctaagtttgc tttttgcatt
    10501 tgtcttagat tatattccta gtaaagagat tttaggtttg ctcggcttga ttccaatttt
    10561 cctaggcatc aaagttttgc tgttaggaga ttctgatgga gaggctattg ccaaagaggg
    10621 tttgcgcaaa gataataaaa acctgatttt tctagtcgct atgattactt ttgcaagttg
    10681 tggtgctgac aatattggtg tctttgttcc atattttact accttaaatt tagcgaattt
    10741 gatagtggct ttacttacct ttctagtcat gatttatctc ttggtttttt ctgcccaaaa
    10801 attagcacaa gtcccttctg ttggagaaac tttggaaaaa tatagcagat ggtttgttgc
    10861 tgttgtttat ttgggattgg gggtatatat cctgattgaa aacaacagtt ttgatatgct
    10921 atggactgtg tcgggccagg aaaaaatatt atgaaaaaga tagtatctgc aaagactgcc
    10981 atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat gccgtctgaa gcagtctctg
    11041 cacgaccttt gtgcgaatat ttgctacact tggcaatccg tccatttctt ctcacgctta
    11101 tgctgaccta cactccgccc gatgcccgcc cgcccgccaa aacccacgaa aagccgtggc
    11161 tgctgctgtt gatggcgttt gcctggttgt ggcccggcgt gttttcccac gatttgtgga
    11221 atcctgccga acctgccgtc tataccgccg tcgaagcact ggcaggcagc cccacccctt
    11281 tggttgccca tctgttcggt caaaccgatt tcggcatacc gcccgtgtat ctttgggttg
    11341 ccgccgcgtt caaacatttg ctgtcgccgt gggcagccga cccgtatgat gccgcacgct
    11401 ttgccggcgt gtttttcgcc gttgtcggac tgacttcctg cggctttgcc ggtttcaact
    11461 ttttgggcag acaccacggg cgcagcgtcg tcctgattct catcggctgt atcgggctga
    11521 ttccagttgc ccatttcctc aaccccactg ccgccgcctt tgccgccgcc ggactggtgc
    11581 tgcacggtta ttctttggct cgccggcgcg tgattgccgc ctcttttctg ctcggtacgg
    11641 gctggacgct gatgtcgttg gcagcagctt atccggcagc atttgccctg atgctgccct
    11701 tgcccgtact gatgtttttc cgtccgtggc aaagcaggcg tttgatgttg acggcagtcg
    11761 cctcacttgc ctttgccctg ccgcttatga ccgtttaccc gctgctcttg gcaaaaacgc
    11821 agcccgcgct gttcgcgcaa tggctcgacg atcacgtttt cggtacgttc ggcggcgtgc
    11881 ggcacgttca gacggcattc agtttgtttt actatctgaa aaacctgctt tggtttgcat
    11941 tgcctgcgct gccgctggcg gtttggacgg tttgccgcac gcgcctgttt tcgaccgact
    12001 gggggatttt gggcattgtg tggatgcttg ccgttttggt gctgcttgcc gtcaatccgc
    12061 agcgttttca ggataacctc gtctggctgc ttccgccgct tgccctgttc ggcgcggcgc
    12121 aactggacag cctgaggcgc ggcgcggcgg cgtttgtcaa ctggttcggc attatggcgt
    12181 tcggactgtt tgccgtgttc ctgtggacgg gctttttcgc catgaattac ggctggcccg
    12241 ccaagcttgc cgaacgcgcc gcctacttca gcccgtatta cgttcccgac atcgatccca
    12301 ttccgatggc ggttgccgta ctgttcacac ccttgtggct gtgggcgatt acccgcaaaa
    12361 acatacgcgg caggcaggcg gttaccaact gggcggcagg cgttaccctg acctgggctt
    12421 tgctgatgac gctgttcctg ccgtggctgg acgcggcgaa aagccacgcg ccggtcgtcc
    12481 ggagtatgga ggcatcgctt tccccggaat taaaacggga gctttcagac ggcatcgagt
    12541 gtatcggcat aggcggcggc gacctgcaca cgcggattgt ttggacgcag tacggcacat
    12601 tgccgcaccg cgtcggcgat gtacaatgcc gctaccgcat cgtcctcctg ccccaaaatg
    12661 cggatgcgcc gcaaggctgg cagacggttt ggcagggtgc gcgtccgcgc aacaaagaca
    12721 gtaagttcgc actgatacgg aaaatcgggg aaaatatata aaaaacaaca gattgagccg
    12781 aatttctgga ttaagtgccg gaaatcgcgt ataattgcgc gattaaacct ttataaatca
    12841 gccgttttac aggcatcaca caggagcgac aattattatg atgaccctct attccggcat
    12901 tacctgcccc ttcagccacc gctgccgctt cgttttgtac gaaaaaggca tggattttga
    12961 aatcaaagac gtcgatattt acaacaagcc cgaagacctc gccgtcatga acccgtataa
    13021 ccaagttccc gtgctggtcg agcgcgattt ggtgctgcac gagtccaaca tcatcaacga
    13081 atacatcgac gaacgcttcc cccatccgca gcttatgccc ggtgatcccg ttatgcgcgg
    13141 caggggccgg ctggtgctgt accgtatgga aaaagaattg ttcaaccacg tccaagtttt
    13201 ggaaaacccc gccgccacca acaaggaaca ggcaaaagcg cgcgaagcca tcggcaacgg
    13261 tctgaccatg cttgcccctt cgttcagcaa aagcaaatac atcctcggcg aagatttttc
    13321 tatgattgat gtcgcccttg ctccgctgct gtggcggctc gaccactacg atgtcaaact
    13381 gggcaaaagt gccgcgccgc tgctcaaata cgccgagcgc atcttccagc gcgaagcctt
    13441 tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa taagtccgaa atgcttgcaa
    13501 aacccaccgt tttgcaggca ttttcctatt ttggcgtaca acacggaacc catcatgccc
    13561 acctcaacca aaccctacgt cctccgcgct ctctgcgaat ggtgcagcga caacagcctc
    13621 acaccgcaca tccttgtttg ggtcaacgaa cacacgcgcg tccccatgca gtatgtccgc
    13681 gacaatgaaa ttatgctcaa catcggtgcg accgccacgc aaaaccttca aatcgacaac
    13741 gattggatca gcttttccgc ccgtttcggc ggacaggcgc acgacatatg gatacctgtc
    13801 ggacacgtcc tcagcctttt cgcacgggag accggcgaag gtatggggtt tgaattggag
    13861 gagtaccgcc ccgacacgcc gcctgaaaac acctctgccg aaaccgcgcc ccgacccgcc
    13921 aaaaaaggct tgaaattggt caaataaatc tatgccgtct gaacggaatc gtgtttcaga
    13981 cggcattttg tccgatgggg cgcaaacgga atccgtttat cggcaaaacc tgtttcggcg
    14041 tattgaaacc gtgttgcccc tgccgattcg atattgggac attgaaaatg ctgtctgaac
    14101 ctgcgatacg ggcttcagac ggcattttgt ccgatattcg ggcaatcagg cggtcagcac
    14161 ggctttcagg attttgttga cttcgcccat atcggctttg ccggcaagtc gggttttcaa
    14221 tacgcccatc actttgccca tatccgccat acctgccgcg ccggtttcgg caacggcggc
    14281 atcgaccgca gtacggattt cgccggcgga gagcatttgc ggcaggtagc ggtgcagtac
    14341 ctcgatttcg gcgttttctt tgtctgccaa atcctgacgg ccggcttcag tgtagatttt
    14401 cacgctgtct ttgcgctgtt tgaccatttt ggtcaggatg gcggtgattt tggcatcgtc
    14461 ggcttcggtg cgttcgtcca cttcaaactg tttgacggcg gcgttgatga ggcggatggt
    14521 gccgagggaa acttggtctt tggcgcgcat cgcggttttc atgtcttcgg taaggcggat
    14581 tttcaggctc ataatgttct cgctgggatg tcggatggaa acggcgggtt tcgatgccgt
    14641 ctgaaaagca aaacacaccg cagagcaggt cttgcggtgt gtcctcatat cacagggctg
    14701 acctgtaatc tgtacttgaa cgtttagtac attttgggcg gcagttgttg gctgcgcagg
    14761 cgtttttgca ggcgttttac ggctgccgct tttttgcgtt tgcgttcggt agtcggtttt
    14821 tcgtaggctt cgcgggcgcg cagctcggtc agcaggccgg ttttttctac ggcgcgtttg
    14881 aaacggcgca tagcgacttc aaagggttcg ttttctttta cgcggattgc aggcatttta
    14941 tttcctttaa taaattctgt tgttgcatct gcccatcata tcggtggaaa gggtagggca
    15001 gacggtgttg aaggtttacc gtatcgctgt gccttgcggc ggcgggatac gcgccgtgac
    15061 ggaaacatca cctcctaacg ggtcggcttg tgccgtgtca agctgcttga aaaagcagga
    15121 aaaacaattt tcggattgtc ttatatttat ggttcgccgt caatgccgtt cgggataaaa
    15181 atgccgtctg aaagaccggg cgggtttcag acggcatcgg tacgtcagcg tgcaggaaca
    15241 atgcccatac gctgtttgag cgggatgtgc ggcgcgccga agagggcggc gtagtaggcg
    15301 gcattggtca tcactttttt gacatagtcg cgcgtttcgg aaaacgggat ggtttcggca
    15361 tataccgcgc cttcgagggg cgtgtccgcc tgccatcggc gcgccctgcc gggaccggcg
    15421 ttatagcctg cggtggcgag gacttcgttg ttttgcaggc ggcgtttggt gtccgccata
    15481 taccacgtcc ccatacggat attgccgtcg gcggtgtaaa attgtgcggc atccataccg
    15541 attttgccgg cgatttcgcg cgcggtggca ggcataacct gcatcagccc ctgcgcgcct
    15601 acgtgggatt gcgcgcctat aacgaagcgg ctttcctgac gaatcagacc gtaaacccaa
    15661 gccggatcga cattaacatt ttgcgcgtgg cggattaccg tgtctttaaa cggcgaaata
    15721 tagcgcaagg tgtagttgag tttgcggtcg gtgcgttccg cgctgttgac cgccatatcg
    15781 taaaaaccgt ggtcgaacgc ggtttgcgcg gcggtcagca gcttgtcttc gtcaaagccg
    15841 cgtgtggcaa aacgccattc cgcctgagcc tgacggcgca ttttcgcatc gccggcggtt
    15901 cggctgtttt ggaacagtac cagcgcgcgc ttaatcgcgc cgtcttccgc catacggagg
    15961 acgctgtttt tgccggcatc gggaacattg ttgcgcgtat cgattttgcg ccccaactct
    16021 tcccctgcca gtacggcata aaaattcctg cccgtcgcta ccgcctgttt gtagagtttt
    16081 tccgcctctt gcgtgttgcc cgttgcggcg cggctgcgtg ccagccagta gagccaggtc
    16141 gggctttttt gcagtttttc gggcatatgc gagattacgg aagccagctc gtcccaacgt
    16201 cgggcgcgca aggcggcgcg ggcgtaccac tcgatttggt cgtcggtcag ttggcggcgg
    16261 tcggcaacct tgccgtaata gtccaaggcg gcaggcacat tgaggttttg cgactgataa
    16321 tgccccaata cgccccacgc gaaactgcgt tgttcgaggc ttaaaccgct ttccatttcg
    16381 gacagcaggg cggcggcatt cggcgatttg cgtgcttctt tgccgatgac gttcaacagg
    16441 gcatattcgc gcgaaccttg tgtaccgccg tcaaacgggc tgcccaatgc ggcggcaagg
    16501 ttgcgtgcgt ctgtggtttg gcggccggcc agcagtccgc gcacgcgcct ccaggcgtcg
    16561 ttgccgtcca acaagccgga tgcggctgcc tgttccaaca gtttggtgca gcccgaaggc
    16621 agtttgcccg tatttttgac cagttcggcg gcacgcgtat agtcgttgcg gctcgaatcg
    16681 gcgtagcatt cgacttcttg ggcgcgccct tcaggtttga gtttggcata ttcctgtgca
    16741 aacagcgtcc actgtctgcg tgcgcccaaa gacttcagcc actcgttgcg gacattttcc
    16801 gccatcgcgc tgtcgccggc gttttccaaa taggcggcga cggcggcatc gtttttctgt
    16861 ttcactgcat ccagtgcgga agggtagctg ctgtaatctg ccagcgtttt tccttcgggt
    16921 tcggcagggc gggtgggaac gcttgccgaa agaccggcag tttctatatt gtctgccagg
    16981 gtcttgctgc tgggcgcgtg tgtcgaagaa cacgcggcaa gcaccagggc cgccagcagc
    17041 ggcagggaat gcttcataga gggtaggtac atcggatttc cttaagaatc ggaaccctga
    17101 acggtcaggg ttggaaaaga caaaatgccg tctgaacagg cgtttgcccg aattatatgc
    17161 cgaaactgca ccgcctttgg aatgtttccg acataattta tagtggatta aatttaaacc
    17221 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
    17281 agtggatcgg ttccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt
    17341 gttaatccac tatatgtttt tcaattattt gccgttttgg tgcgaaccgc tgcctttgcc
    17401 cgtttcagac ggcattgtcc gaaatggttg cccgcttcct gctttattga caaaaaaatg
    17461 ctttcccgat aatatcctac gaaaattaac ctgccgattt gacacagctt gcgggcataa
    17521 cagctaaagc gttccgacaa tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg
    17581 gctttgttgt atggaaaaga caatgattat tttggacaag gtttccaagc attaccaaac
    17641 gcgcgacaag acccgttttg ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga
    17701 aatcttcggg ctgatgggtt attcgggtgc aggcaaatcc accctgttgc gcctgattaa
    17761 cctgttggaa cgccccgaca gcggcaaggt caacgtctgc ggacaagagc tgaccgcgct
    17821 tgatgccgcc gcattgcgtc aggcgcggca gaatatcggc atggtatttc aacagttcaa
    17881 cctgttgaac aaccgcaccg tcgccggcaa cgtcgccttc ccattggaaa tcgccggatg
    17941 gccgtctgaa aaaatcaaaa cgcgcgttgc cgaatgcctc gaaatcgtcg gactgaccga
    18001 acgcgccggc cactatcccg cccagctttc cggcgggcag aaacagcgtg tcggcatcgc
    18061 ccgcgcactc gcgcccaaac cccaagtcat cctcgccgac gaacccactt ccgccctcga
    18121 ccccgccacc acgcgcagcg tcttggaatg tttggaagac atcaacaaac gattcaacgt
    18181 aaccatcgtc atcgtaaccc acgaaatgag cgtcatcaga cgactgtgcg accgcgccgc
    18241 cctgttggac aaaggcaaag tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc
    18301 ccgatccgac atcgggcgcg aactgattcg ggaggactga tatggcagac ttaacattcc
    18361 aacaagccgt ttccaacatc atcggtatga aaggcgaaat cgtccgcgca ttgggcgaaa
    18421 ccttcgtgat ggtcggcttg tccaccacat tcgccgtcgt tttcggcacg ttgctgggcg
    18481 tgttgctctt cgtaacttcc agccgccaac tgcattacaa caaggcggtg aacttcctgc
    18541 tcgacaacct cgtcaacctc atgcgcgcct tccccttcgt cattttgatg attgcgatga
    18601 tacccgccac acgcgccatc gtcggcagca ccatcggtcc ggttgccgcc tcgctggtgt
    18661 tgagcgtgtc gggattgttt tattttgccc gtttggtgga acaaaacctg cgcgaagtcc
    18721 ccaaaggcgt gattgaagcc gccgccgcta tgggtgcgcc gccggttgcc atcatccgca
    18781 aagtcctctt aaacgaagcg cgcgcgggca tggtttccag cattaccgtg cttgccatcg
    18841 ggcttttgtc atacagcgcg gcggcaggga tgataggcgg cggcggcttg ggcgacctcg
    18901 ccatccgcta cggctactac cgctaccaaa ccgaagtcat cgtcttcatc gtcgccctcc
    18961 tcgtgctgct ggtcatcctg attcaaagca ccggcaacgc gttggcgcgg aaactcgaca
    19021 aacgttgaac ccaaatgccg tctgaacgcc aaaaccccca ccgctatccg aaaaatgcta
    19081 taaaatcccc ctgttcgcgg caaatgccgt ctgaacgccg aatccggacg gcaggactcc
    19141 ctgcccgtca tttttgtttg aaactgccac aacatcagga gaaaatatga aaaccttctt
    19201 caaaaccctt tccgccgccg cactcgcgct catcctcgcc gcctgcggcg gtcaaaaaga
    19261 tagcgcgccc gccgcatccg cttctgccgc cgccgacaac ggcgcggcga aaaaagaaat
    19321 cgtcttcggc acgaccgtcg gcgacttcgg cgatatggtc aaagaacaaa tccaacccga
    19381 gctggagaaa aaaggctaca ccgtcaaact ggtcgagttt accgactatg tgcgcccgaa
    19441 tctggcattg gctgagggcg agttggacat caacgtcttc caacacaaac cctatcttga
    19501 cgacttcaaa aaagaacaca atctggacat caccgaagtc ttccaagtgc cgaccgcgcc
    19561 tttgggactg tacccgggca agctgaaatc gctggaagaa gtcaaagacg gcagcaccgt
    19621 atccgcgccc aacgacccgt ccaacttcgc ccgcgtcttg gtgatgctcg acgaactggg
    19681 ttggatcaaa ctcaaagacg gcatcaatcc gctgaccgca tccaaagcgg acattgccga
    19741 aaacctgaaa aacatcaaaa tcgtcgagct tgaagccgcg caactgccgc gtagccgcgc
    19801 cgacgtggat tttgccgtcg tcaacggcaa ctacgccata agcagcggca tgaagctgac
    19861 cgaagccctg ttccaagaac cgagctttgc ctatgtcaac tggtctgccg tcaaaaccgc
    19921 cgacaaagac agccaatggc ttaaagacgt aaccgaggcc tataactccg acgcgttcaa
    19981 agcctacgcg cacaaacgct tcgagggcta caaatcccct gtcgcatgga atgaaggcgc
    20041 agccaaataa ggcagtcgta taaaatgatg ccgtctgaac cgtatccgtg ttcagacggc
    20101 atttttgtcc tttaatccgc cattccctgc cattccgccg aatccggcgt atcgattccg
    20161 aacagcgaca aagcgtgtgc aacactgtgc gccactatgt cgtccgccgt ctgcggtttg
    20221 cggtacatcg caggaacagg gggaaacacc acgccgccca tttccgttac ccgcttcata
    20281 ttgtccaaat gggcaaggtt cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt
    20341 tccttcaaaa ccacatccgc cgcacgcgtc aagaggttgt cgccgaagcc gtgcgcgacc
    20401 gaggcaagcg tccgcatcga acagggggcg accagcatcc cgtccgtttt aaacgtaccg
    20461 ctggcaatgc acgccccgat attgccgatc ggatgcacga agtccgcgag ggcatatacc
    20521 tcgtctctcg cataagccgt ttccgaagcg cgcgccatct ccgcaccttt cgataccaca
    20581 aggtgcgttt cgatatcttg cgcgcgtaaa agttccaaag ccttcacgcc gtattggaaa
    20641 ccgctcgccc cactgatgcc gattatcaaa cgccgtacca tcatccgcct ttcccataaa
    20701 accgcctgca acggcaaacc ggctattata gtgaaaaaac agaaatccga taaatacgga
    20761 tacaaattgt cggcaacacc caatatccga taaaatatcc gatttaacat cctatctgaa
    20821 taggcacggg agggcggtat ggcaaaagta aaaggcggat tggggcgcgg tttggattcg
    20881 ctgctcgcca acggcgcaga caacagcagc ggcgaccgat tgaccacggt tgcggttaaa
    20941 gatatccggc ccggccgcta tcaggcgcgt gttcaaatcg atgacgaagc cttgcaggag
    21001 ctggcagatt cgattaaggc gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga
    21061 ctgtcccgat acgaactgat tgcaggcgaa cgccgttggc gcgccgcaca gattgccggc
    21121 ctgaccgaaa tccccgccgt tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt
    21181 ttgatcgaaa acctccagcg cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa
    21241 cgccttgccg acgagttcgg gctgacccac gaaaccatcg cccaagccgt cggtaaaagc
    21301 cgaagcgcga tttccaacag cctgcgcctt ttaagcctgc ccgaacccgt gcaggaaatg
    21361 ctttaccaac gccgcctcga aatggggcac gcccgcgcac tgctgaccct gcccgtcgtc
    21421 gaacagctcg aattggcgca aaaggctgtc aaaaacggct ggtcggtgcg cgaagtcgaa
    21481 cgccgcagcc aagccgccct tcaaaacaaa cgtcccgagc ccaaaaaaac tgccgccgcc
    21541 gacatcggcc gcctgaatga tttgctgact gaaaaactgg gtgtcaacgc cgaaatcaaa
    21601 accgccaacc ataaaaaagg cagaatcatc ctgcatttcg acacgcccga aaccttcgac
    21661 catatcttga agcaattggg catagattac cgaccttaat tttgcgggat ataccgtctg
    21721 aaatatagag aatagctttc cagattttaa gtgggaaata taattctatt gacatttttt
    21781 ctgcttcacg taagaatcgt tttcctgttt tcatttttaa ttttcgagga aattatgaac
    21841 acacgcatca tcgtttcggc tgcgttcgtt gcgttggcat tagcaggttg cggctcaatc
    21901 aataatgtaa ccgtttccga ccagaaactt caggaacgtg ccgcgtttgc cttgggcgtc
    21961 agcccaaatg ccgtaaaaat cagcaaccgc aacaatgaag gcatacgcat caactttacc
    22021 gcaactgtgg gtaagcgcgt gagccaatgc tatgttacca gtgtaatcag cacaatcggc
    22081 gttaccactt ccgatgcaat ttgtttggga ggcggaacgc acaaaggcaa aagtcaatgc
    22141 aatgctttgc ttaaagcggc aggccgttgc taatccttta ttcggaaaag gtcgtctgaa
    22201 aatattttca gacgaccttt tatttattga gcaaattccc caaactgccg acaatccgac
    22261 cgataaactg ctacaatttc gcgttgattt tttattattt ccacgtaaag caaacaaatc
    22321 acgtaagaac attaaaagtg taaagaaatt gcggacaaac tccttaatat cctgtaatta
    22381 tatgtaatgt gtgttagaat gacacctgtc gccgttattc aggcggcggg tgcggcggat
    22441 tgggatacgt tgtttcacaa agattaactg tcgcttgacg taataaggct tctaaattat
    22501 attagccgcg ccccaaatcc gaggcggaaa cattcccttt atgaagcaga ttatcatcct
    22561 acaatccgcc gtattgtcca tatgcgccgc agttgccttt gctgtatggg gttttgccgg
    22621 attcctttcg gcggtcggtg gcggtttgtc ctacctgctt cccacgtttg ttgcagtttt
    22681 acttttaaaa cttttcaggg gaaacccctt cctgcaaagc aggatgttcg tcttcggaga
    22741 gattttaaaa gtagtgctgt cgctgttgtc catgcttgcc gtatttgcaa tatggcatca
    22801 atcgctggtg ttcgccccgt ttctgatggg gctgctcggt gtcagccatt tggttttttt
    22861 agtattgttg agagtgaaag attatggcag gtgaaaccat taccgctgcc gactacatca
    22921 agcaccactt gcagagcttg accagtttgt cggatgttac tcagggtcag ggactgaaaa
    22981 acattgctga tttttcgttt attaaccttg atgccgtctt ttttgccgtc ctgttgggcg
    23041 taatcggcag ctttctgttg tggcgcggtg caaaaaaagc gacggcaggc gttcccggac
    23101 gtttccaggc cgcagtggaa atcttgtttg agtttgtgga cgatatgtgt aagagcatca
    23161 ttcacaacga aaagtctaga aaagccgtcg cgccgttggg tctgacgctg tttgtctgga
    23221 tttttctgat gaacgcgatg gatatgctgc cggtcgattt gctgccgatg gtatggcagg
    23281 gcattaccgg caaccatcac gccctgctgc gcgtcgtacc gactgccgat ttgaacacca
    23341 ctttggcact cgctgtcggc gtgttgctga tttgtatcta ttacaacatc aaaatcaaag
    23401 gattgggtgg ctggtttcac gagttgttca gcgcgccgtt cggtgcaaaa ctcgcacctg
    23461 cgaacttcct gttgaatttg gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt
    23521 tgttcggcaa tatgtatgcg ggcgaattgg tattcttgct gatagccttg cttggcggtg
    23581 cttgggcggc ttccggcagc gtcgaagtca tggatccgat tctgtttgta ttccacatta
    23641 ttgccggttt ggcatgggcg attttccata ttttggtgat taccctgcag gcgtttattt
    23701 tcatggcgtt ggcgttcgtc tatatcggac aggcacatga tgcgcactga ttttcctaag
    23761 tagtttgttt ttgtagttta acctttgttt ctttaaggag tttaaaatgg gtttgattgc
    23821 tatcgcatgt ggtttgatcg ttgcattggg tgcattgggt gcctctatcg gtatcgcaat
    23881 ggtcggttct aaatatttgg agtcttctgc ccgtcagccc gaactgatcg gtccgctgca
    23941 aaccaaactg ttcctgattg ccggtttgat tgatgccgca ttcttgattg gtgtcgccat
    24001 tgcactgctg ttcgccttcg ttaacccgtt tgcaggtgca taatcaggac ggattcgtcc
    24061 gttccgtaca ggcgcagcct gtttgattaa ccccagtacg aaggttaagt aacgtgaata
    24121 tcaatgcaac attattcgct caaatcatcg tctttttcgg tttggtatgg tttaccatga
    24181 aattcgtgtg gccgccgatt gcaaaagctt tggatgagcg tgccgcaaaa gtcgccgaag
    24241 gcttggctgc cgccgagcgt ggtaaaagcg atttcgagca ggctgaaaaa aaggttgcag
    24301 aactcttggc agaagggcgt aatcaggttt ccgaaatggt tgccaacgcc gaaaaacgtg
    24361 ccgccaaaat tgtcgaagaa gccaaagaac aggcttcttc cgaggcggcg cgcattgcag
    24421 ctcaggcaaa ggccgatgtg gagcaggaat tgttccgcgc acgcgaatcc ctgcgcgagc
    24481 aggttgccgt gttggctgtc aaaggtgccg aatctatttt gcgcagcgaa gtcgatgctt
    24541 ccaaacacgc aaaactgctc gataccctga aacaggagct gtaatcttat ggcagagttc
    24601 gcaacgattg ccagacctta tgcaaaggca ttgttcggtc tggctcagga aaaaaaccaa
    24661 attgagtctt ggttgggcgg actggaaaaa cttgcggcgg ttgttcagga agggaaggtg
    24721 gcttcattga ttgaccgtcc tgaaacgaat gcttcagaaa aagcagatat cctcatcgat
    24781 ttggtcggtt tgaaagacaa ggagttgaaa aactttgtta ttgtcttggc cgggcagaaa
    24841 cgtttgtcga tattgccgga agtatatgct caatatcaag acttgacctt atcattcaac
    24901 catatcaaat ctgccgtcat ttacagtgcc tatccgttga ccgacaaaca ggtcggcgag
    24961 ttggcgcaaa tgctgaataa gcgtttcgac agcgagctga aaatctctgt cgaaatcgaa
    25021 ccggagctga ttggcggcat aaaagttgaa gtgggtgatc aggttttgga tttgtctgta
    25081 caaggcaaat tgagtgcctt gtacacgact atgacgaatt aggagagttt tcatgcagct
    25141 taatcctgct gaaattagcg atttgattaa agccaagatc gaaaatctgt ctgtaaatgc
    25201 cgaagtgcgt actcgtggta ccgttgtttc tgttacagac ggcattgtgc gcattcatgg
    25261 cttgtcagac gcgatgcaag gtgagatgct cgaattcccc ggtaacactt tcggtctggc
    25321 catgaacttg gagcgcgact ccgtcggtgc cgtagtgttg ggtgagtacg aacacattaa
    25381 agaaggcgat acagttactt gtaccggtcg tatcttggaa gtgccggtcg gacgcgaact
    25441 ggtcggacgc gtcgttgatg cattgggtcg gcctatcgac ggcaaaggcc cgattaatac
    25501 aactttgacc gcccctatcg aaaaaatcgc accgggcgtg attgcgcgca aatcggttga
    25561 ccagccgatg caaaccggtc tgaaggcgat tgactctatg gttcctgtcg gtcgcggtca
    25621 gcgtgagttg attattggcg accgtcagac aggtaaaacg gccgtagcat tggatgccat
    25681 cgtcaaccaa aaaggtacgg gtgttatctg tatctatgtc gctatcggtc aaaaagcatc
    25741 ttctattgcc aacgtggtcc gcaaattgga agagcatggc gcgatggagc ataccatcgt
    25801 ggttgctgca actgcatctg aagcggcggc attgcaatat atcgcacctt actccggttg
    25861 tacgatgggt gaattcttcc gcgaccgcgg cgaagatgcc ttgattgttt atgacgattt
    25921 gtccaaacag gctgtggctt accgtcaaat ttccctgctt ttgcgccgtc cgcccggccg
    25981 cgaagcctat cccggcgatg tgttctacct gcactcccgt ctgttggaac gtgcagcacg
    26041 tgtcaatgaa cacgaagtgg agaaattgac caacggcgaa gtaaaaggca aaaccggttc
    26101 tctgactgct ttgccgatta tcgaaaccca agcgggcgac gtatctgctt tcgttccgac
    26161 taacgtcatt tcgattaccg acggtcagat tttcttggaa accgacctct tcaacgccgg
    26221 tatccgtcct gcaatcaatg ccggtatttc cgtatcccgc gtaggcggtg ctgcacaaac
    26281 caaagtgatt aaaaagctgg gtggcggtat ccgtttggcg ttggcgcaat atcgtgaatt
    26341 ggcggcgttc tcgcaatttg catccgattt ggatgaagct acgcgcaaac aattggagca
    26401 tggcgaagtc gtaaccgaac tgatgaaaca gaaacagttc agtacgttga atacggctga
    26461 aatggctttg accctttggg caatcaacaa cggttcgtat tctgatgttc cggttgccaa
    26521 agctctggct tttgaatctg aatttttgag ctttgtccgt acccaacatc cggaagtttt
    26581 ggaagccgtc aatgcttcag gtgcaatgtc cgacgagagc gagaaaacgc ttgaagcagc
    26641 catgaaatcc ttcaaatctt cttacgccta tcaggcataa ggcttgaagt gaaaggagtc
    26701 tgaaatggca gtaggaaaag agattctcac caaaatccgc agtgttcaga atacccaaaa
    26761 gatcactaaa gcgatgcaga tggtgtcaac ctctaaaatg cggaagactc aggaacggat
    26821 gcgtttggcg cgtccgtatg ccgaaaaagt gcgtatggtg atgagccatc ttgcgcaaac
    26881 caataccgat catggtattc cgttactgga atctcatcgg gaaatcagac gtgtcggttt
    26941 tattttgatt acgtctgata agggtttgtg tggcggtttg aacgccaacg tgctgaaaaa
    27001 gtttttggca caagttcaag agtatcggaa tcaaggtatt gaagaggaaa tcgtatgcct
    27061 tggcagtaaa ggtctgatgg cgtgtcagag cattggtctg aatgtggttg ccagtgccgt
    27121 aaatttgggc gataccccaa aaatggaaat gctgctcgga cctttgacag aactcttcca
    27181 acggtatgag aaacatgaaa ttgacagaat ccatctggtg tattcgggtt ttgtcaatac
    27241 catgcgtcaa gaaccgagaa tggaagtatt gctgcctatc ggtgagaacg tgattggcga
    27301 ttcagctccc aaatcaccgt tcagctggga ataccgctac gaaccgactg cacttgcagt
    27361 gttggaatat ctggttcgcc gctatttaga gtctgtggtt tatcaggcgt tgagcgacaa
    27421 tatggcatcc gaacaggcag cccgcatggt tgccatgaaa gctgcaacag acaatgcagg
    27481 caatgccatc aaagagttgc gtttggtata taacaaatcg cgtcaagctg cgattaccac
    27541 ggaattgtca gaaattgtag caggtgcggc ggctgtctga tgccgtctga atcctgtaca
    27601 gaaattagga tacgataatg agccaaggca aaatcgtaca aattatcggt gcggttgttg
    27661 acgtggaatt tccacgcgac atgattccgc gcgtttacga cgctttgaaa ttagacgaaa
    27721 acggtctgac tttggaagtc caacagcttt tgggcgacgg cgtagtccgt accattgcga
    27781 tgggcagctc ggacggtttg aaacgcggca tgactgtgag caataccggt gcgcccatta
    27841 ctgtgccggt aggtaaaggt acattgggtc gtattgtcga cgtattgggt acgcctgttg
    27901 acgaggcagg tccgatcgat accgacaaga gtcgtgccat ccaccaagcc gctcctaagt
    27961 ttgacgaact gtcttccaca accgaattgc tcgaaacggg cattaaagtg attgacttgc
    28021 tgtgtccgtt tgccaaaggc ggtaaagtag gtctgttcgg cggtgccggt gtgggtaaaa
    28081 ccgtgaacat gatggaattg atcaacaaca tcgccaaagc gcacagcggc ttgtccgtgt
    28141 tcgcaggcgt gggtgagcgt acccgcgaag gtaacgactt ctaccacgag atgaaagatt
    28201 ccaacgtatt ggataaagtg gcaatggttt acggtcagat gaacgaacct ccgggcaacc
    28261 gtttgcgcgt cgcattgacc ggtttgacca tggcggaata cttccgtgac gaaaaagacg
    28321 aaaacggcaa aggccgcgac gtattgttct tcgtggacaa catctaccgt tacactctag
    28381 ctggtaccga agtatccgca ttgttgggcc gtatgccgtc tgcagtgggc taccaaccga
    28441 cattggcaga agaaatgggt cgtttgcagg agcgtattac ctctacccaa accggttcta
    28501 ttacttctat ccaagccgta tatgtacctg cggacgactt gaccgacccg tctccggcaa
    28561 caacgttcgc ccacttggac gcaaccgtcg tattgagccg cgatattgcc tctttgggta
    28621 tttacccggc agtcgatccg cttgattcca cttcgcgcca actggatccg atggtcttgg
    28681 gtcaagagca ctacgacgtg gcgcgcggcg tacagtccac cctgcagaaa tacaaagaat
    28741 tgcgcgacat catcgccatc ttgggtatgg acgaattgtc tgacgaagac aaactgaccg
    28801 taatgcgtgc gcgtaaaatc caacgcttcc tgtcccaacc gttccacgtt gccgaagtgt
    28861 ttacaggttc tccgggcaaa tatgtcgccc tgcgcgatac cattgccggc ttcaaagcca
    28921 tcttgaacgg cgaatacgat catctgcccg agcaggcatt ctatatggtc ggcagcattg
    28981 aagaagcggt tgagaaagcg aaaaccttaa actgaggagg tcggcatgag catcatgcaa
    29041 gttgaggtgg taagtagcga gcagaaaatc tattcaggcg aggcaacatt tatcgttgtt
    29101 ccgactgtac agggcgaact cggtatttat ccgcgacacg agccgattat gagtttggtg
    29161 cggccggggg ctttgcgttt gaccgttccg ggcgaggata aagaggtttt ggttgctgtt
    29221 tccggcggtg ttttggaagt acagcctgat aaagtaactg tcttggcgga tgttgccgtc
    29281 cgcagtgcag agatggatca ggcacgtgcg gaagaggcga aaaaagccgc agaagcgggc
    29341 atttcccaag ctaaagacga taaggctttg gcggaggcgc ataaagcatt ggctgctgcg
    29401 attgcacagc ttaaaacttt ggactatatc cgttcgcaca agaaataaac ttgtttggtt
    29461 tggaaaagca cgaccgtttg gtcgtgcttt ttattttccg gatttaaatt aaagactgtg
    29521 tttggatatg gcgttggcag gcatagcctg ctttaaggta ttcaataggg cagcctgccg
    29581 cctaaccgaa acatatagcg gattaacaaa aatcaagaca aggcgacgaa gccgcagaca
    29641 gtacaaatag tacggaaccg attcacttgg tgcttgagca ccttagagaa tcgttctctt
    29701 tgagctaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat catgtatgcc
    29761 gtgttttcaa atcagtctgt ctttgagata aacctatcca aacagcaatg gaaacagtaa
    29821 aataaaccct ttattttatc gaatcataag ccatgcttac cttccaacaa atcatcttca
    29881 aactgcaaac cttctgggca gacaaaggct gcaccgtcat ccaacccttc gacatggaag
    29941 tcggtgccgg cacatcccat cccgccacct gcctgcgcgc gctcgggccc gagccttggt
    30001 ttgccgccta cgtccaaccc agccgccgcc ccaaagacgg ccgctacggc gacaacccca
    30061 accgcctgca acactattac caattccaag tcgccctcaa acccgccccc gccaatatcc
    30121 aagaccttta tctcgactcc ctgcgcgaat tgggcatcga ccccaaagtg cacgacatcc
    30181 gcttcgtcga agacgactgg gaaaacccca ccctcggcgc atggggcttg ggctgggaag
    30241 tttggctcaa cggcatggaa gtaacccagt ttacctattt ccaacaagtc ggcggcatcg
    30301 actgcacgcc cgtactcggc gaaatcacct acggcatcga acgcttggcg atgtacctgc
    30361 aaggcgtgga aaacgtctat gacctcgttt gggcaaaaac gccggacggc aacaccgtaa
    30421 gctacggcga cgtgtaccac caaaacgaag tcgaacaatc cacctacaac ttcgaataca
    30481 gcgatgccga ttggctgctg cgccagttca acgactacga agcgcaagcc aaacgcctgc
    30541 ttgccgaaga aaacgccgcc ctcgccttgc ctgcctacga gctggttctc aaagcggggc
    30601 atacgttcaa tcttttagac gcacgcggcg cgatttccgt aaccgagcgg gcaacttata
    30661 ttggacgtat tcgtgcattg agccgcgccg tggcgcaaaa atatgttgaa agccgcgaaa
    30721 aactgggctt ccctttaatg aaggcaaatg ctgcctaagg gggcgacgat tttttacgtt
    30781 cgggcggcac gataaggcag ccgtccgtaa accattatat attttaggaa aatcatgcct
    30841 gtctatttcc gcaccccaag caggaaacgc ctgcacaaaa tgaaccgccg ccagcataaa
    30901 aagctgcatt tgggcgagtt tcaaacattg attttcggcg ttcaaggcag tttgcaccaa
    30961 gctgaggacg atgccgaagc gttcgaccgc tttatggacg agctgattgc gtatcttgag
    31021 gtaaacgact ttggaatggc gggcgggggc gattgcggcg atttttcgtg cctgtttcaa
    31081 aaatccggtg cccgaagctt gggcgaaacc gagaaaaaag ccattatctc ttggctggca
    31141 ggccgcgaag atgtcgccac tttgcacacc accccgctgg ttgacggttt ttacggggat
    31201 gacggctggg aaaattacag tgaaacctat aaacaggcag attaaccaaa tttaaagaaa
    31261 gccgaatgat gacccaaacc cttttaatcg aactccttac cgaagaactc ccgcccaaag
    31321 cactgaataa tctgggcaac cattttgccg ccgccgttgc cgaaggcttg gaaaaagcgc
    31381 aactggttga cggtgcagcc gaatttacgg cttatgcctc gccgcgccgt ttggcggttc
    31441 aagtcaaaaa cgttaaagcc gttcaggccg accaaaaaat cgtgaaaaaa ggccctgccg
    31501 tggcgaatgc catgaaagac ggtgcgccga ccaaggcttt ggaaggtttc gcacgcggtg
    31561 cgggcgcgaa aatcgaagac ttgaccatcg tccacgacgg caaacaggat gtgtacgcct
    31621 acgaatacgt ccaaaccggc aaaccgctgg gcgaactttt ggaagacatt atcaatcaag
    31681 ccgttaagaa actgccgatt ccgaaagtga tgcgttgggg cagcagcacg tttaccttcg
    31741 tgcgccctgt tcacgggctg attgtgctgc acggcggcga catcgtaaac gtcagcgttt
    31801 tgggtctgca aagcagcaat caaaccttgg gacaccgctt cttgtccaac ggcgaaatca
    31861 ccattgaaaa cgccgacagc tacgccgaac aaatgcgcgg gcaaggcaaa gtcgtcgctt
    31921 cgtttgccga gcgcaaagcc gcgattcaga cggcattgga agggcaggcg cgcagtctga
    31981 acgccgccgt cgccgccgat gaagcattgt tggacgaagt aaccgcgctg gtcgaatggc
    32041 ctgttgtttt ggaagccggt tttgaggaac atttcctcgc cgtgccgcag gaatgcttga
    32101 ttttaacgat gcagcaaaac caaaaatact ttccgctgct cgaccaaaac ggcaagctga
    32161 tgaaccgctt cctgctggtt tccaacctgc aaaccgaaga cccgtcgcac atcatccgag
    32221 gcaacgaacg cgttttgcgc gcgcgcctgt ctgatgccga gttcttctac aaacaagacc
    32281 aaaaagcgac tttggaaagc cgcctgccga aattgtccag cgtggtgtac cacaacaaaa
    32341 tcggctcgca agccgaacgc atcgaacgcc tgcaaagcat tgccgcccac atcgccaaag
    32401 cattgggcgc ggatgccgcc gctgccgaac gcgccgcgcg tcttgccaaa gccgacttgg
    32461 taaccgaaat ggttggcgag ttccccgaac tgcaaggcac gatgggcaaa tactatgcac
    32521 gtttggacgg cgaaaccgaa gaaatcgccg aagccatcga gcagcactat cagccgcgtt
    32581 ttgccggcga caagctgccc gaaagcaaaa ttgccgccgc cgtggcactg gcggacaagc
    32641 tggaaaccct ggtcggcatt tggggcatcg gtctgattcc gaccggcgac aaagacccct
    32701 acgccctgcg ccgcgccgcc ttgggtattt tgcggatgct gatgcagtac ggtttggacg
    32761 tgaacgaact gattcagacg gcattcgaca gcttccccaa aggtttgctc aacgaaaaaa
    32821 cgccgtctga aaccgccgac tttatgcagg cgcgccttgc cgtgttgctg caaaacgatt
    32881 atccgcaaga catcgtcgcc gccgtactcg ccaaacagcc gcgccgtttg gacgatttga
    32941 ccgccaaact gcaagccgtt gccgcgttca aacagctgcc cgaagccgcc gcgctcgccg
    33001 ccgccaacaa acgcgtgcaa aacctgctga aaaaagccga tgccgcgttg ggcgcggtta
    33061 acgaaagcct gttgcaacag gacgaagaaa aagccctctt tgccgccgcg caaggcttgc
    33121 agccgaaaat cgccgccgcc gtcgccgaag gcaatttcca aaccgccttg tccgaactgg
    33181 cttccgtcaa accgcaagtc gatgcattct ttgacggcgt gatggtaatg gcggaagatg
    33241 ccgccgtaaa acaaaaccgc ctgaacctgc tgaaccgctt ggcagagcaa atgaacgcgg
    33301 tagccgacat cgcgcttttg ggcgagtaac cgttgtacag tccaaatgcc gtctgaagcc
    33361 ttcagacggc atcgtgccta tcgggagaat aaattgcagc ctttagtcag cgtattgatt
    33421 tgcgcctaca acgtagaaaa atatttcgcc caatcattag ccgccgtcgt gaatcagact
    33481 tggcgcaact tggatatttt gattgttgat gacggctcga cggacggcac acttgccatt
    33541 gccaaggatt ttcaaaagcg ggacagccgt atcaaaatcc ttgcacaagc tcaaaattcc
    33601 ggcctgattc cctctttaaa catcgggctg gacgaattgg caaagtcggg ggggggggga
    33661 tatattgcgc gcaccgatgc cgacgatatt gcctcccccg gctggattga gaaaatcgtg
    33721 ggcgagatgg aaaaagaccg cagcatcatt gcgatgggcg cgtggctgga aattttgtcg
    33781 gaagaaaagg acggcaaccg gctggcgcgg caccacaaac acggcaaaat ttggaaaaag
    33841 ccgacccggc acgaagacat cgccgccgtt ttccctttcg gcaaccccat acacaacaac
    33901 acgatgatta tgaggcgcag cgtcattgac ggcggtttgc gttacgacac cgggcgggat
    33961 tgggcggaag attaccaatt ttggtacgat gtcagcaaat tgggcaggct ggcttattat
    34021 cccgaagcct tggtcaaata ccgccttcac gccaatcagg tttcatccaa acacagcgtc
    34081 cgccaacacg aaatcgcgca aggcatccaa aaaaccgcca gaaacgattt tttgcagtct
    34141 atgggtttta aaacccggtt cgacagccta gaataccgcc aaacaaaagc agcggcgtac
    34201 gaattgccgg agaaggattt gccggaagaa gattttgaac gcgcccgccg gtttttgtac
    34261 cgatgcttca aacggacgga cacgctgccc gccggcgcgt ggctggattt tgcggcagac
    34321 ggcaggatgc ggcggctgtt taccttgagg caatacttcg gcattttgca ccgattgctg
    34381 aaaaaccgtt gaaaaacgcc gctttatcca acagacaaaa aacaggataa attatgcaaa
    34441 accacgttat cagcttagct tccgccgcag aacgcagggc gcacattacc gataccttcg
    34501 gcgtgcgcgg catcccgttt cagtttttcg acgcactgat gccgtctgaa aggctggaac
    34561 aggtaatggc ggaactcgta cccggtttgt cggcgcaccc ctatttgagc ggagtggaaa
    34621 aagcctgctt tatgagccac gccgtattgt ggaagcaggc attggacgaa ggtctgccgt
    34681 atatcaccgt atttgaggac gacgttttac tcggcgaagg cgcggagaaa ttccttgccg
    34741 aagacgcttg gctgcaagaa cgctttgacc ccgattccgc ctttatcgtc cgcttggaaa
    34801 cgatgtttat gcacgtcctg acctcgccct ccggcgtggc ggactacggc gggcgtgcct
    34861 ttccgctgtt ggaaagcgaa cactggggga cggcgggcta tatcatttcc aaaaaagcga
    34921 tacggttttt cctggacagg tttgccgccc tgccgcccga agggctgcac cccgtcgatc
    34981 tgatgatgtt cagcgatttt ttcgacaggg aaggagtgcc ggtttgccag ctcaatcccg
    35041 ccttgtgcgc ccaagagctg cattatgcca agtttcacga ccaaaacagc gcattgggca
    35101 gcctgatcga acacgaccgc ctcctgaacc gcaaacagca atggcgcgat tcccccgcca
    35161 acacattcaa acgccgcctg atccgcgcct tgaccaaaat cagcagggaa agggaaaaac
    35221 gccggcaaag gcgcgaacag ttcattgtgc ctttccaata aaaggaggag agatggacat
    35281 cgtatttgca gcagacggca ggatgcggcg gctgtttacc ttgaggcaat acttcggcat
    35341 tttgcgccga ttgctgaaaa accgttgaaa aacgccgctt tatccaacag acaaaaaaca
    35401 ggataaatta tgcaaaacca tgttatcagc ttagcttccg ccgcagaacg cagggcgcac
    35461 attgccgcaa ccttcggcgc gcgcggcatc ccgtttcagt ttttcgacgc actgatgccg
    35521 tctgaagaac tgaatcggat gatggcggaa ttggtgccgg gattggcaaa acagcacctg
    35581 ttgagcgaag tggaaaaagc ctgctttatg agccacgccg tattgtggaa acaggcattg
    35641 gatgaaggtc tgccctatgt tgccgtattt gaggatgatg tcctgcttgg caaagacgca
    35701 gaaaagttcc ttgccgaaga tacttggttg gaagagcgtt ttgataagga ttctgccttt
    35761 atcgttcgtt tggaaacgat gtttgcgaaa gttattgtca gaccggataa agtcctgaat
    35821 tatgagaacc ggtcatttcc tttgctggag agcgaacatt gggggacggc tggctatatc
    35881 atttcgcgtg aggcgatgcg gtttttcttg gaaaggtttg ccgttttgcc ggcagagtgg
    35941 attaaagcgg tagattggat gatgtttact tatttctttg ataaggaggg gatgcctgtt
    36001 tatcaggtta atcccgcctt atgtacccaa gaattgcatt atgccaagtt cctcagtaaa
    36061 aacagtatgt tgggtagcga tttggaaaaa gatagggaac aagaaagaag acaccgccgt
    36121 tcgttgaagg tgatgtttga cttgaagcgt gctttgggta aattcggtag ggaaaagaag
    36181 aaaagaatgg agcgtcaaag gcaggcggag cttgagaaag cttacggcag gcgggtcata
    36241 tcgttcaaat agtttgtgta aaatatagcg gattaaaatt agaaatggat acac
//
LOCUS       DAXIHS010000010        36067 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXIHS010000010 DAXIHS010000000
VERSION     DAXIHS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.010"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(29..391)
                     /locus_tag="ACFOF2_000413"
     CDS             complement(29..391)
                     /locus_tag="ACFOF2_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC7106793.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            complement(669..1538)
                     /locus_tag="ACFOF2_000414"
     CDS             complement(669..1538)
                     /locus_tag="ACFOF2_000414"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC7106794.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQVGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1633..2550)
                     /gene="hisB"
                     /locus_tag="ACFOF2_000415"
     CDS             complement(1633..2550)
                     /gene="hisB"
                     /locus_tag="ACFOF2_000415"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC7106795.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(2547..3644)
                     /gene="hisC"
                     /locus_tag="ACFOF2_000416"
     CDS             complement(2547..3644)
                     /gene="hisC"
                     /locus_tag="ACFOF2_000416"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC7106796.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3690..4979)
                     /gene="hisD"
                     /locus_tag="ACFOF2_000417"
     CDS             complement(3690..4979)
                     /gene="hisD"
                     /locus_tag="ACFOF2_000417"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC7106797.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4976..5875)
                     /locus_tag="ACFOF2_000418"
     CDS             complement(4976..5875)
                     /locus_tag="ACFOF2_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC7106798.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5979..6632)
                     /gene="hisG"
                     /locus_tag="ACFOF2_000419"
     CDS             complement(5979..6632)
                     /gene="hisG"
                     /locus_tag="ACFOF2_000419"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC7106799.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6711..7364)
                     /locus_tag="ACFOF2_000420"
     CDS             complement(6711..7364)
                     /locus_tag="ACFOF2_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC7106800.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7640..7724
                     /locus_tag="ACFOF2_000421"
     tRNA            7640..7724
                     /locus_tag="ACFOF2_000421"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7674..7676,aa:Leu,seq:caa)
     gene            8433..10160
                     /gene="ilvB"
                     /locus_tag="ACFOF2_000422"
     CDS             8433..10160
                     /gene="ilvB"
                     /locus_tag="ACFOF2_000422"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC7106801.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            10171..10662
                     /gene="ilvN"
                     /locus_tag="ACFOF2_000423"
     CDS             10171..10662
                     /gene="ilvN"
                     /locus_tag="ACFOF2_000423"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC7106802.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            10729..11022
                     /locus_tag="ACFOF2_000424"
     CDS             10729..11022
                     /locus_tag="ACFOF2_000424"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC7106803.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            11102..12115
                     /gene="ilvC"
                     /locus_tag="ACFOF2_000425"
     CDS             11102..12115
                     /gene="ilvC"
                     /locus_tag="ACFOF2_000425"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC7106804.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12339..13334)
                     /locus_tag="ACFOF2_000426"
     CDS             complement(12339..13334)
                     /locus_tag="ACFOF2_000426"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC7106805.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(13476..16061)
                     /gene="acnB"
                     /locus_tag="ACFOF2_000427"
     CDS             complement(13476..16061)
                     /gene="acnB"
                     /locus_tag="ACFOF2_000427"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC7106806.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            complement(16371..17441)
                     /gene="lptG"
                     /locus_tag="ACFOF2_000428"
     CDS             complement(16371..17441)
                     /gene="lptG"
                     /locus_tag="ACFOF2_000428"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC7106807.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(17438..18553)
                     /gene="lptF"
                     /locus_tag="ACFOF2_000429"
     CDS             complement(17438..18553)
                     /gene="lptF"
                     /locus_tag="ACFOF2_000429"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC7106808.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            18723..20129
                     /locus_tag="ACFOF2_000430"
     CDS             18723..20129
                     /locus_tag="ACFOF2_000430"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC7106809.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            20192..20632
                     /locus_tag="ACFOF2_000431"
     CDS             20192..20632
                     /locus_tag="ACFOF2_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC7106810.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            20891..21697
                     /locus_tag="ACFOF2_000432"
     CDS             20891..21697
                     /locus_tag="ACFOF2_000432"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC7106811.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKAEAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            21804..22430
                     /gene="purN"
                     /locus_tag="ACFOF2_000433"
     CDS             21804..22430
                     /gene="purN"
                     /locus_tag="ACFOF2_000433"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC7106812.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNRETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            22446..23117
                     /locus_tag="ACFOF2_000434"
     CDS             22446..23117
                     /locus_tag="ACFOF2_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC7106813.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            23235..23657
                     /locus_tag="ACFOF2_000435"
     CDS             23235..23657
                     /locus_tag="ACFOF2_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC7106814.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            24226..24942
                     /locus_tag="ACFOF2_000436"
     CDS             24226..24942
                     /locus_tag="ACFOF2_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC7106815.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            25091..25993
                     /locus_tag="ACFOF2_000437"
                     /pseudo
     CDS             25091..25993
                     /locus_tag="ACFOF2_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
     gene            26036..26809
                     /locus_tag="ACFOF2_000438"
     CDS             26036..26809
                     /locus_tag="ACFOF2_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC7106816.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TVEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            26921..28609
                     /locus_tag="ACFOF2_000439"
     CDS             26921..28609
                     /locus_tag="ACFOF2_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC7106817.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFADFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            28702..29658
                     /gene="gshB"
                     /locus_tag="ACFOF2_000440"
     CDS             28702..29658
                     /gene="gshB"
                     /locus_tag="ACFOF2_000440"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC7106818.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVSYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            29970..30353
                     /locus_tag="ACFOF2_000441"
     CDS             29970..30353
                     /locus_tag="ACFOF2_000441"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC7106819.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            30646..31119
                     /locus_tag="ACFOF2_000442"
     CDS             30646..31119
                     /locus_tag="ACFOF2_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC7106820.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            31245..32348
                     /gene="mnmA"
                     /locus_tag="ACFOF2_000443"
     CDS             31245..32348
                     /gene="mnmA"
                     /locus_tag="ACFOF2_000443"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC7106821.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            32419..34089
                     /locus_tag="ACFOF2_000444"
     CDS             32419..34089
                     /locus_tag="ACFOF2_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC7106822.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            34201..35835
                     /locus_tag="ACFOF2_000445"
     CDS             34201..35835
                     /locus_tag="ACFOF2_000445"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC7106823.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     TGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         8497 a         9202 c         9837 g         8531 t
ORIGIN      
        1 aaaatcccct aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggtct
       61 ctttgtcgct gaattcctgc gcgctttctg ttaaaggtgc ggcatgggct ttgccttttt
      121 cggtcagcga cagcaaccgt tcgcgccggt cctgttcgct ttcctgccat tcaatcaacc
      181 cttgtccggc aagggttttg catacgcctg aaacggtctg tttgggcagg ctccactttt
      241 cgccgatatg cttttgcgtg cggctgcctt cggttgccag ggtataaagt acggcaaaga
      301 ggttgtaatt cagatcctgc tgcccgatcc atttgtcgaa gacattgcaa atcaggttga
      361 tacgggtgcc aagttggtcg agttggttca taattggtct tgatattgac taaatttgtg
      421 cggattatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagagaa
      481 cgattctcta aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtcttc
      541 ggcttcgtcg ccttgtcctg atttttgtta atccactata tagtcataat cgggactgat
      601 tttaagcgtg gggtgataaa aatgccgtct gaaatatgtt cagacggcat tgtgtttgga
      661 aaaggcaatc agtgttctgc caatttcagg taaacgccgg aaacgtcctg ttcgccgtag
      721 ccggcttcga ctgctttgcg gtagctggca gcaacggttt cgacggcggg cagggtgttg
      781 cctacctgtt caagctcttt gacggcgagg ttgaggtctt tggaggcgtg tttgagggcg
      841 aaggcgggtg ggaattcgcg gtttgcccac agggattttt tggtttggaa catgggcgag
      901 tccattgccg agccgccgat ggcttcgacg atggtgtcgg tatcgatgcc gaactgccgc
      961 gccatcagca tcgcttcgct gtacgcttcg ccgaaaatgc ccaagagcga gttcaagacg
     1021 agtttcgcgc ccgaaccttt gccgacatcg ccgaaatgga aggttttttt gccgacgagg
     1081 gaaaatattt tttgcagcgg gtttaaaacg gcttcgctgc cgccgaacag aatcagcagc
     1141 gtgccgttgg tggcgggccc gaccgatccg gaaacgggtg cttcggcaaa ctgtccgcct
     1201 gcggcttcga caagtgcttt gacggcgagg ttttcggtcg gggagatggt gctcatgttg
     1261 acgatgattt tgccggccaa tccgtcgcgg actccgttca ggatgtcgca cacggcggca
     1321 tagtcggaaa ccatcaggaa aatgacggga tagtcgcgga cgagttcggc ggtgttgccg
     1381 taaacttttg cgcctttggc ggagatgggg gcagttttgt cgggcgagcg gttgtatacg
     1441 ccgacttcga tgccgccgtc caagagccgc gttaccatag gcagacccat ttgccctaag
     1501 cctatccagc cgatttgtgt gtattcgttt gcggacatga tgtttactcc ttttctgttt
     1561 gtagtgtagt gtaatcttat ctgttccgat accgtctgaa aagcctgcgg gtgtttcaga
     1621 cggtatggtt ttttatgcgg tcagcgtgcc tttggtcgag ggggtctgtc ctgccatgcg
     1681 cgggtcgtgt tcgactgcca tacgcagggc gcgcccgaag gctttgaata cggtttccgc
     1741 ctgatggtgg gcgtttttgc cgctgaggtt gtcgatgtgc agggtcatca tactgtggtt
     1801 gacgatgccg tggaaaaatt cttcaaacaa atcgacatcg aaacgtccga ttagtgcgcg
     1861 ggtaaattcg atgttgtaca cgagtccggg gcggccggaa aggtcgatga cgacgcggct
     1921 gagggcttcg tcgagcggga cgtaggaatg tccgtaacgg cggatgcctt ttttgtcgcc
     1981 gagtgcctgc cggattgctt gtccgagtgt gatgccgatg tcttcggcgg tgtggtggtc
     2041 gtcgatgtgc aggtcgcctt tgcagctgat gtcgatgtca atcatgccgt ggcgggcgat
     2101 ttgatcgatc atgtgttcga ggaagggtac gccggtatcc agcctgcttt tgccgctgcc
     2161 gtcgaggttg atggagacgg tgatttgggt ttcgcaggta ttgcggttga cggtaacgct
     2221 gcggcagccg gcagcagatg cggtttcggc aatttgggtt tcggcagttc cggtgctttc
     2281 tgcggcggtt tcggggacag tgcgttcgcg gtgttttctg tcgagccagc ctttgggttt
     2341 gccggtgtgt ttttcgagtt ttgccatcag gctgggacgg atgccgcgtg catttgggtc
     2401 ttctgcctgc ttttcaaggc gttgtttgag tttgtagagt gcgacggggg tacggtagcc
     2461 gcagagtttg gcgagcttgg acagcgaacc tgcttcttgg gcgagggtca gaaagttgtg
     2521 cagttggcgt tgtgttttag tcaaattcat aggaaatccg ttggttggca gtaaagttgg
     2581 cgaatgatgt tgagaacggc atcgttttgt gcggggctgc ctacggtaat gcgcaggcag
     2641 tgttccaaaa gcgggtgcgc gccatgcagt tttttaacca agatgcggtt ttgtttgagc
     2701 gtgtcaaaca acaaatcggc atcgggtacg cgtatggtaa tgaagtttgc ctgacttgga
     2761 aaggtgttca gacggcatat tttgcccaat tcggcgaaca tccgttcgcg ttcgtttttc
     2821 aggctgtcga tgttggcaga gatgatgccg tagtgttgca gggcgagttt ggcggtggtc
     2881 aggctcagtt ggttcatatt gtagggcggc aggatttttt gcagttcgcc gatgacttcg
     2941 ggacagcctg ccgcatagcc gatacgcagt ccggcaaaac cgattttgct gacggtacgc
     3001 atgacgatga ggttgggaat gctgcccgcc tgcggcagga agctgtcgcc gttgaatgcg
     3061 ccgtaggctt cgtcgacgac gacgatgccg tctgaagctt cgatgacggc ttcgatttcg
     3121 gcacgcgtga aacatacgcc ggtggggttg ttggggtagg cgataaaggt cagggcaggg
     3181 cggtgcttcc tgacggcttc gaggacggcg ggcaggttga gggtgaaatc tccgttcagt
     3241 ggaacgccga cataatccat gccgtacagc gcggcgttgt ggcggtacat gacgaaactg
     3301 ggttcggctg ccaacattgc cgcgcccggt ttggcggtca gcatcgtgat gaactgtatc
     3361 agctcgtccg agccgttgcc cagcgcgatg tcggcgcagt cgggaatgtc gaacgccgaa
     3421 cgtaatgctt cctgtaaacc gctgccggag ggattggggt aaagatggat gggcgcggcg
     3481 gcaagccgtg cctgccattc ctgcaacagc gtttcatgtc cggcaaaagg gtggacggga
     3541 ctttccatcg aatcgagttt ggcaaagccg ggcggaacgt cggcaatctg atatgccgac
     3601 atagcttgta tgtcgtcgcg gatgaaggag cggacggatt tcatcgtgtt tccttaatgg
     3661 ttggaatatg cctgtacgcc gtttcggcat tatttcatac ggaactctgc cgcgcgggcg
     3721 tgggcggtca ggctttcgcc gtgtgccagc acgctggcgg tttcgcctaa tttttgcgcg
     3781 ccctgttccg aaacctgaat caggctggag cgtttttgga aatcatatgt ccccaaaggc
     3841 gaggaaaagc gggcggttcg gctggtgggc aatacatggt ttggaccggc gcagtagtcg
     3901 ccgaggcttt cgccggtgta gcgtcccatg aaaatcgcac cggcgtggcg gatttttttc
     3961 gcccattcct gcggattttc gactgacagt tccaagtgtt cgggggaaat gtagttggcg
     4021 atttcgcagg cttcgtccaa gtctttggcg agtatcatcg cgcccctgtt gccgagcgag
     4081 gcttcgatga tgtcgcggcg cggcatagtt tcgatcaggc ggtccatagc ggcttctact
     4141 tcgtcgagat acgcttgcga cgtgccgatt aagatggctt gggcaatttc gtcgtgttcg
     4201 gcctggctga acaaatccat cgccacccaa tcggcaggtg tcgtgccgtc ggcgatgacc
     4261 aggatttcag acggccccgc caccatgtcg atgccgacca cgccgaacac gcggcgtttg
     4321 gcggcggcga cgaaggcgtt gcccggaccg gtgattttat cgacttgggg gatggtttcc
     4381 gtgccgtagg cgagggcggc aaccgcctgc gcgccgccga cggtgaagac tttggttacg
     4441 ccggcgacgt atgcggcggc aagcacgatg tcgttgcgtt cgcctttcgg tgtcggcacg
     4501 accatgatga tttctttcac gcctgcgacg tgggcgggca tggcgttcat gatgacggaa
     4561 ctcggatatg ccgccttgcc gccggggacg taaatgccga cgcggtcaag cggggtaatc
     4621 tgttgtccca acagcgtgcc gtcttcatcg gtgtagctcc acgattccat tttttggcgt
     4681 tggtggtagc tttcgacacg gcgggcggcg gtctgcaatg ccgtctgaac gtcgttcgga
     4741 atgcgctcga acgccgcgtt caaatcggct tgcgtgagta ttaaatcatc gatgctttta
     4801 gcgtttgtct gatcgaattt gttggtgtat tcaatcaaag ccgcatcgcc gcgcttttgc
     4861 acgtcggcgc aaatgtcggc gacgatgcgt tcggtttcgg ggttttgcgc ggtttcaaaa
     4921 gccagcaggg ctttgagtcc ggcttggaaa tcgggcgatt gggtgttgag ttttttcatg
     4981 atttggtttt ttcctttttt atttttggat tgggggtcgt ctgaatattc tcttgttcag
     5041 tttctgtctc tgttttgggt ctgatatacg ctttttctcc cccgctacca aacttcaatt
     5101 cgggcaggca tttttgttgc agaccgagtt tggctaatac ctcttcgcta cgagaggtag
     5161 tgcggaaacg gtaacgggat tgttttctac cgtttttggt ttcctcaacc attgtttcaa
     5221 aaccgtccac ttcgtatgca ctgacaacag cgttttgcag accggtgtga acgccgataa
     5281 cgtatttcac ttttgaagca acatctttac cgataaccca gttgcccaac gtacgcgatt
     5341 tgaggttggc atcgtcttgg ttgtcgaaaa ggtagtctaa ttcttcgtca gtatctaaat
     5401 cgaaagcatt gtggattttg atggcgagaa tcagcccgtc ggggttaatc tcgttaagtg
     5461 ggagagaaga gaatccaaag cggcgatcta gttcttctac gctaatacca cccggaccat
     5521 gcccggcaat tttatttttg agttttttac ccaagacaga tttaacaaaa tgaattaagg
     5581 cagattcggc ggcgagtgct tcgacttcag tcagatgata gctgatgata tagcgaccga
     5641 gtttcttgca tttggagatg gctttgagtt tccgatcgat aatctcgccg gagactgggt
     5701 cttgtgaacg actagcaacc cattcatgct cgaagatacg attaccgcag cctttgccga
     5761 tgtagaggat tttgtctttt ttcaggtcgg tcagacaata gacataaaac cgacgctcgc
     5821 cgttattcaa aacggaaagg gttgaatctg agaatttttt tatttttgca accattttaa
     5881 cttctcctat acagtttact gccggtgata ttttcagacg acctgtcggc gggaacggat
     5941 agggtcgtct gaaaacgcat ctttattcaa atggatgctt acttcgcctt cactgcgccg
     6001 ccgaacgcct gaataatcgg ctccagcagc gcgtatttcg ttttcaaagc agccttgttg
     6061 accaccaggc ggctggaaat gtcgacgatg tgttcgactg cttccaagcc gtttgccttc
     6121 aaggtgttgc ccgtcgaaac caagtccaca atcgcatcgc tcaagccgac cagcggcgca
     6181 agttccatcg agccgtacag tttgataatg tccacatgga cacccttgcc ggcaaaatga
     6241 gatgccgcga tttcaggata cttcgtggca atcttcagac ggcatccggg ttgcgaagct
     6301 gcttcgtaat caaacccttt acgcacagca accatcatgc ggcacttggc aatctccaaa
     6361 tccaaaggcc ggtaaagccc cgtgccgccg tgttcgatca gcacgtcttt gcccgcaatg
     6421 ccgaagtccg ccgcgccgta gcggacataa gtcggcacat cggttgcgcg gacaatgaca
     6481 aggcggatgt tttcatggtt cgtcccgata atcagcttgc gcgatttttc aggctcttca
     6541 gtcggaacaa tgccggcagc ggcaagcagc ggcagcgtct cctcaaaaat gcgccccttg
     6601 gataaggcga tggtcaaagc attatcctgc atggtgtgga tgtcctttgt cggttgaaac
     6661 aataatgccg tctgaatagg cgccgacggt gcagacggca tacggtttta tcagagcagg
     6721 gtccttacat cggcagcaat cgtttccggc tcgcttccgt aaggcgagaa aatggcaacc
     6781 tcaccgtttt tgtcgatgag atacgcaccg gaagagtggt cgaccaaata gttttcgctg
     6841 tcgtcttttt gattgacttt ggcagaaacc acgcggtatt gctgcttgat gaccggcagg
     6901 ttttggccgc ccgttgccgt cagaccgata aagtccggat tgaactgttt ggcatacttg
     6961 ccgatgattt caggcgtgtc gcgttccgga tcgatgctga cgaacaccac tttcacgtcc
     7021 ttagcctgcc cgcccaactg cttcaacgtg tcgctgtacg tcaaaagctc tgtcgggcag
     7081 acatcgggac agtgcgtaaa gccgaaagac agaatcacga ccttgccttt caaatcgctc
     7141 aggttgaaag gcttgccttc gccgtcggtc agcgtgaaat cgccgccgat gtcttcctta
     7201 cgcatatccg taccgcgcgt ttgcggcttt gccgcatttt ccgcagctga cgcggatgca
     7261 cttgaagaag cggcttgcgc cgcactgttg tcttgaggtt tgcaggcggc aagcgcggca
     7321 agtacgaaaa cgcccggcaa aaaggaacgc ggtacggaaa acatgacaat ctcccaagaa
     7381 taaatagcaa aacctgattc gtacggaatt tgaatatttt catcttaacg cgtttggcag
     7441 atttaatgta gtattttttt acgttgtctg cacggcagga attgccgaat catacagaat
     7501 ctttggagcg gaaagaacag taagattaaa ataataaaaa ttctatttaa aatcaaaaat
     7561 aaaataaaaa cagataaaat aatttcaaaa aaaggtttac agaatttcaa aagccattat
     7621 aatgacgact tcttcaccag cccaggtggc gaaattggta gacgcagggg actcaaaatc
     7681 ccccgccgca aggtgtgtcg gttcgagtcc gaccctgggc accacaaccg cttttggagc
     7741 ggttattttt ttgtctattg ctgtttaatg aaatagataa gactaaatat agtggattaa
     7801 atttaaacca gtacggcgtt gcctctcctt gccgtactat ttgtactgtc tgcagcttcg
     7861 tcgccttgtc ctgatttttg ttaatccact atacaatcaa tatattaagt cgttgttagc
     7921 cctcttggtt ctgtcaatgc cgtccaatac agtccggtgc aatgagccat tttcaggaag
     7981 tatggttggg aatatttttg atatatcaaa aatgaaatca agttcaatca atatcccgag
     8041 tctttattgg gatgaaaccc tgtaaatttt acaattcttg atatcgaaga agggtttttc
     8101 acatctgccc ctcctacaca aactgtcaac aatttccatc tttcaaaaaa ggtactcctg
     8161 attttattct tttaaaatca atcaataatt tctctgcttt caaaaaatat taaaagatga
     8221 aagatttttc ttgattgaac tgtcggtttg cgttatcgtt tccactcttt ttcacaaact
     8281 ctgtgttcct ttccaattga ttggataatc cgtctgttgc cgtgttcccc caatgcgcgt
     8341 accctaaatc gctgcttggt ggaattgcat tacaggtgct gtggcaaagc ggtttgtccc
     8401 tattggtttg aaaccgtata aaagaggtcg ttatgcagct atcaggcgcg caaatcatag
     8461 tgcagagtct caaagccgaa ggtgtcgagt acgttttcgg ttatcccggc ggtgcggtta
     8521 tcgaaatcta cgatgccctt ttccaactca ataaattcaa gcacattttg acccgtcacg
     8581 agcaggcggc agtacacgcg gcagatgcgt atgcgcgcgt cagcggtaag gtgggcgtgg
     8641 cattggttac atccggcccg ggcgttacca atgcactgac cggtattgct actgcctata
     8701 cggattcgat tccgatggtg gtcatcagcg ggcaggtagg caattccctg attggtacgg
     8761 atgcgttcca agaagttgat acggtgggta ttacccgtcc gtgcgtcaaa cacaatttct
     8821 tggttacgga tatcaatgag ttggcggaaa ccattaaaaa ggcgttccaa attgccgcaa
     8881 gcggccgacc ggggcctgtg gtggttgatg tcccgaaaga tgttacgcag gcgatggcga
     8941 aattcagcta tcctcaggaa gacattttta tccgttcgta tcaaccggtt gttcaagggc
     9001 atatcggtca gattaaaaag gccgtgcaga tgttggcatc tgccaaacgt ccagtcgtct
     9061 attttggcgg cggcgtggtg ttgggtaatg cttctgaaga gctgacccga tttgtccgaa
     9121 tgacgggtgc tccgtgcacg ggtacgctga tgggcttggg cgcttatcct tccagcgacc
     9181 gccaattcct cggtatgctc ggtatgcacg gtacttacga ggcaaacctt gccatgcaga
     9241 atgcggatgt cgttcttgcc gtaggtgcgc gttttgacga ccgtgtggtt tccgtaccgt
     9301 ccaaattttt cgagaaggcg aagaaggtta tccatatcga tgtcgatcct tccagcatcg
     9361 ccaaacgcgt gaaggcggac attccgattg tcggcgacgt gaaaaacgtt ttgtccgaga
     9421 tggttgcgct gtggcaaaaa caagagtccg tgccgtctga agatactttg ggcaaatggt
     9481 ggaaaaccat agaggaatgg cgttcccgag attgcttgtg gtttgacaac ggcagcgaaa
     9541 ttatcaagcc ccaatatgtg attcagaagc ttgccgagat taccggcaat tcggcaatca
     9601 tcacatcgga tgtagggcag catcaaatgt ttgcggctca atattatccc ttcgaacgtc
     9661 cgcgccaatg gctcaattcc ggcggtttgg gtacgatggg cgtgggtctg ccttatgcga
     9721 ttggtgcaaa acttgccgcc ccggatcaag acgtattctg tattaccggc gacggctcga
     9781 ttcagatgaa catccaagag ttgtccacct gtttccaata tcggattccg gttaacgtca
     9841 ttacgctgaa caacggttat ctcggtatgg tacgccagtg gcaggaaata tattacggcg
     9901 gccgagagtc ggaaacttat ttcgattctt tgcccgattt cgtcaaactt gccgaggcat
     9961 acggtcatat cggcatccgc gtggacaaga agtctgatgt ggaaggtgcg ttgttggaag
    10021 cattgaacca aaaagacagg ctggtgttta tcgacttcct gaccgaccag aaacagaatg
    10081 tgatgcccat ggtcggtaac ggcaaaggtt tggacgaaat ggtacttccg ccgcatatgc
    10141 gtgcggacga aaaggcgtaa ggagaggcaa atgcgacata tcttatctgt tctgattgaa
    10201 aacgaatcag gtgcgatgag ccgcgtggtc ggtttgttct ctgcacgcga ttacaatatc
    10261 gattctttgg cggttgcgcc gaccgaagac aaaaccctgt cccgtatgac catcgttacc
    10321 cacggcgacg agcaggtcat cgaacaaatc accaagcaac tcaataaatt gattgaagtg
    10381 attaaagtgg tcgatttgaa cgaaagccgt tttgtcgaac gcgaactgat gttggtaaaa
    10441 gtccgtgccg ccggcaaaga ccgcgacgaa tttttacgct tgaccgaaat ctaccggggc
    10501 agcatcatcg acgtaaccga ccgcagctat acgattgaaa ttaccggctc gacagacaag
    10561 ctggattcct ttttggaaac ggtcggacgc gcccaaattt tggaaaccgt acgcacaggc
    10621 gcggccggca tcggtcgcgg tgagcgtatt ttgaaaattt aacgccgtaa cctttcagac
    10681 ggcacggtat ttgaatgccg tctgaaaaac gaacggcagg agagatttat gtcgaacatt
    10741 aaaattgtcg cactggttac cgtcaaaccg gaatacacag aaacactggc agcacagttt
    10801 aaagaactgg tcaaagccag ccgtgcagaa gagggcaaca tcagctacga tctccatcag
    10861 gaaatcggca aaccgaaccg ttttgttttc gtggaaaatt ggaaatctca agcagctatt
    10921 gacgaacata acgccagcgc gcatttccaa gccttcgtcc agtccgtcga cggcaaaacc
    10981 gaagcgttgg aaatcgtatt gatgaatgaa gttgccgatt aagcggcaca ctctgtttaa
    11041 cccgtccgaa gccgctcaac attttaaggc ttcgacaacc atttacctta aaggaaatca
    11101 aatgcaagtc tattacgata aagatgccga tctgtcccta atcaaaggca aaaccgttgc
    11161 catcatcggt tacggttcgc aaggtcatgc ccatgccgcc aacctgaaag attcgggtgt
    11221 aaacgtggtg attggtctgc gccaaggttc ttcttggaaa aaagccgaag cagccggcca
    11281 tgtcgtcaaa accgttgctg aagcgaccaa agaagccgat gtcgttatgc tgctgctgcc
    11341 tgacgaaacc atgcctgccg tctatcacgc cgaagttgca gccaatttga aagaaggcgc
    11401 aacgctggca tttgcacacg gcttcaacgt gcactacaac caaatcgttc cgcgtgccga
    11461 cttggacgtg attatggttg cccccaaagg tccgggccat accgtacgca gtgaatacaa
    11521 acgcggcggc ggcgtgcctt ctctgattgc cgtttaccaa gacaattccg gcaaagccaa
    11581 agacatcgcc ctgtcttatg cggctgccaa cggcggcacc aaaggcggtg tgattgaaac
    11641 cactttccgc gaagaaaccg aaaccgatct gttcggcgaa caagccgtat tgtgcggcgg
    11701 cgtggtcgag ttgatcaagg cgggttttga aaccctgacc gaagccggtt acgcgcctga
    11761 aatggcttac ttcgaatgtc tgcacgaaat gaaactgatt gttgacctga ttttcgaagg
    11821 cggtattgcc aatatgaact actccatttc caacaatgcg gagtacggcg aatacgttac
    11881 cggccctgaa gtggtcaatg cttccagcaa agaagccatg cgcaatgccc tgaaacgcat
    11941 tcaaaccggc gaatacgcaa aaatgtttat ccaagagggt aatgtcaact atgcgtctat
    12001 gactgcccgc cgccgtctga atgccgacca ccaagttgaa aaagtcggcg cacaactgcg
    12061 tgccatgatg ccttggatta ctgccaacaa attggttgac caagacaaaa actgattgtt
    12121 ttcaaacggg actacctaca catcgtgtag gcagtttttt tatagtggat taacaaaaat
    12181 caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg
    12241 tactggttta aatttaatcc actatatatg gataccgtct gaacatcgtg ttcagacggt
    12301 atctgtgttg cggatgaatt taaacaggca cagttctgtc agtcgcccag agccgcgacc
    12361 attaccgctt taatcgtgtg catacggttt tccgcctgat cgaacacgat gctggcgggg
    12421 ctttcgaata tttcttctgt aacttccaca ccgttcagcc cgaaggtttc gtaaatccat
    12481 tcgccgactt tggtttcgcg gttgtggaag gcgggcaggc agtgcatgaa tttgacttgc
    12541 ggattgcccg atgccgccat cagttcgggc gtaacgcggt aatctttcag caaatcgatg
    12601 cgttcctgcc agacttcttt cggctcgccc atgctgaccc acacatcggt atgaataaaa
    12661 tcaacattct tcacggcttc atgcgcgttt tcggtcaggg taatttttgc gccggtttct
    12721 ttggcggcgg cgtgtgcggc ggcaataatg ccttcagacg gccacaggct ttgcggtgcg
    12781 ccgatacgca cgtccatccc caattttgcc cctaaaatca gcagggaatt gcccatgttg
    12841 taacgcgcgt cgccgacgta ggcaaacgcg gtttggttca aaggtttgcc gctgtgttcg
    12901 cgcatagtca gtgcgtcggc aagcatttgt gtgggatgga actcgttggt cagcccgttg
    12961 aacacgggta cgcccgcgta tttcgccaat tcttcaacaa cttcctgacc gaaaccgcga
    13021 tattcgatgg catcgtacat cctgcccaac acgcgggcgg tgtctttgat gctttcctta
    13081 tgcccgattt ggctggcgga cggctctaaa taagtcactc ccgcgccttg atcgcgcgcg
    13141 gcgacttcaa acgcgcagcg cgtccgagta gaggtttttt caaaaatcag ggcgatgttt
    13201 ttccctttca tccgctgaat ctcgcgccct gcttttttgg cggctttcaa ttcggcggca
    13261 aggtcgaggt aggcggtgat ttcttccggc gtgaagtcta aaagtttcag aaaatggcgg
    13321 tttttcaggt tcactgtcgt ttcctttatg tgggggcgtg aacgccgtat ttgcagcgtt
    13381 tgcggaaaga agggttagcg gaatgttgca atgccgtctg aaggttgttc agacggcatt
    13441 gcggtcaatc ccttacttca tacggatgac ggggattaaa cgttcacggt ctcggctact
    13501 tcgttgtagc tgtcgatttc gttgaagttc atatagcggt agattttatc gccctgttcg
    13561 ttgatgatgc cgatattggc ttgatattct tcaacggtcg ggattttacc cagtttggag
    13621 caaatcgctg ccaattccgc cgaaccgagg taaacaaagg tgtttttacc caaacggttc
    13681 gggaagttgc gggtggaggt ggacataacg gtcgcacctt cgcgtacttg cgcctgatta
    13741 cccatacata aggagcaacc cggcatttcc atacgcgcgc cggcacgtcc gagtacgccg
    13801 tagtgtcctt cgtcggacaa ttgtttcgcg tccattttgg tcggcggcgc aatccacagg
    13861 cggacggggg tgtctgcctt gccttccaaa agtttggagg cggcgcggaa gtggccgatg
    13921 ttggtcatac acgaaccgat gaatacttcg tcgattttgg tgccggagcg ttcggacatg
    13981 aagcacacgt cgtccgggtc gttcgggcag gcgataatcg gctctttgat gtcgtccatg
    14041 ttgatttcaa tcacggcggc gtattcggca tctttatccg cttcgagcaa ctcgggattt
    14101 gccagccatt tttccatagc tttgatgcgg cgttccaaag tgcgcgggtc ttgatagccg
    14161 ttggcaatca tgtttttcat caacacgacg ttggatttca tgtactcgat aatcggctct
    14221 ttgttgagct tcacggtaca gccggcggcg gagcgttcgg cggatgcgtc ggtcaattca
    14281 aaggcttgtt ccactttcaa atcaggcagg ccttcgattt cgaggatgcg gccggagaag
    14341 atgtttttct taccggcttt ggcaacggtc agcaaacctt gtttgattgc gtacagcggg
    14401 atggcgttca ccaaatcgcg cagggttacg cccggttgca gcttgccgct gaagcgtacc
    14461 aatacagact cgggcatatc gagcggcatt acgcccgttg cggcggcaaa ggcaaccaag
    14521 ccggagccgg cggggaagga aataccgatg gggaaacggg tatggctgtc gccgccggtg
    14581 ccgacggtat cgggcagtag caggcggttg agccacgagt ggatgacgcc gtcgcccgga
    14641 cgcagtgaca cgccgccacg ggtggaaata aaggcgggca gttctttatg ggtttttaca
    14701 tcgacaggtt tcggataggc ggcggtgtgg cagaaagact gcatcaccat atcggcggag
    14761 aagcccaaac aagccaagtc tttcaactcg tcgcgggtca tcgggccggt cgtgtcttgc
    14821 gagccgaccg tcgtcatacg cggttcgcag taagtacccg gacgcacgcc ttgtccttcg
    14881 ggaagaccgc aggcgcggcc gaccattttt tgcgccaagg tgaaaccagc tttgctttcg
    14941 gcaggcgctt gcggcaggcg gaatgcagta gaggcaggca gtttcagggc ttcgcgcgct
    15001 ttagcggtca gacctcggcc gataatcagg ttgatacggc cgccggcttg cacttcgtcc
    15061 agcaatactt gtgatttcaa ttcaaactcg gcaacagtct cgccgttttt cacgattttg
    15121 ccttcataag gcaggatatc gacgacatcg cccattttta gagcagatac atcgacttca
    15181 atcggcagcg cgccggaatc ttcttgggta ttgaagaaaa tcggcgcgat tttgccgccc
    15241 aaacatacgc cgccgaagcg tttgttcggc acgaacggaa tgtcttcgcc ggtatgccaa
    15301 atgacggagt tggtcgcgga tttgcgtgaa gaaccagtac cgaccacgtc gccgacgtaa
    15361 gcaaccggat ggcctttggc tttgagttct tccaacaatt taatcggacc gacttcgccc
    15421 ggtttgtcgg gcgtgatgcc gtcgcgcggg tttttcagca ttgccagcgc gtgcagcgga
    15481 atatcgggac gactccacgc gtcgggcgcg ggggagaggt cgtctgtatt ggtttcgccg
    15541 tcaactttga aaacggtaac ggtgattttt tcgggaactt tggcgcgtga ggcgaaccat
    15601 tcggcatctg cccaagattg caaaacttct tgcgcgtatt tgttgccttt ttcggctttt
    15661 tcctgaacgt cgtggaagga atcgaacatc agaagcgtat gtttcaagcc tttggcggca
    15721 atgggcgcga gtttgtcgtc gtccaagagt tcgattaagg cgtggatatt gtaaccgccg
    15781 agcatcgtac ctaagagttc ggtcgcatat tcgggggaga tcagcgggct ggacgcgctg
    15841 ccttcggcaa cggcagccag gaatgaggct ttgactttgg cggcatcgtc cacaccgggc
    15901 ggaacacggt gggcaagcag ttcgaccaag aactcgcctt cgcctgcggg cgggcttttc
    15961 agcagctcaa ccaaatcggc ggtttgctgc gcgttcaaag ggagggcggg aatgccgagg
    16021 gcggcgcgct cggcggcggc tttacgatag gcttctaaca tctctttgtt cctttttctg
    16081 tttttctttt gtcgggttgc aaatgatttg cgttaaatat tgtaaacaat atttgtaccg
    16141 ctatcataga ctagttttaa caaaaatgga actgttatga caggtaatgt atagtggatt
    16201 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aatagtacgg caaggcgagg
    16261 caacgccgta ctggtttttg ttaatccact ataataggga acggctatct gtctacagga
    16321 atatgataat aaaaaccaat gccgtccgaa gggggtcaga cggcattggt tcaacgtttt
    16381 tcctgtttgc gtatcagcca aacagcgagc aaggcgaagg ctatggtagg tagtgcgccg
    16441 gcgaggaagg gcgggatgcc gtagagttgg ctggtaaacc cgaagagccg tccggcaagg
    16501 tggaacagca atccgagaca gatgccgccg aagagtttta agcccatatt gccgtggcgg
    16561 gtggtttgcg gggtaaaggc aaaggcgacg agcgccatca cccaggctgc ggcggggtaa
    16621 accaatttgc gccaccatgc gatggcgtag attcgggtgt tttggctgtt gttttggagg
    16681 tggcggatgt aggtggtcag ttcgccgacg gacatttggt cgggtttgac gagcaatacg
    16741 tccatcaggt tgcgtttgac ggaaatcggc cagttttctt cagccgcaat agagacctcg
    16801 actttgtctt cgccaagcgt gctgcggcgg atgtttttca actgccaact gccgtcgctg
    16861 ttcaaaacgg cggaatcggc ttccactgcc tctgccaatt cgtttttatc gttgcgcgcc
    16921 caaattttga tgcccaaaag cgtatggtcg ggcaacattt cgcgcacatt gataatgctg
    16981 tttttttctt tcagccaaag gccggtattg ccggtgctga ttttgccgtt gatggcggcg
    17041 gctttgatgt tttcggcttt ttggctcagt gtgggcgcaa cccattcgcc gagcgcgacg
    17101 gtggcaatag caaaaataaa accgaactgc gacagaatca acagcagctt tttggtgctc
    17161 atgccgctgg ctttgatgac ggtcagttcg ctgccggcgg caagctggct gagagagacc
    17221 agtccgccga taaggacggc gaggggcatc agttcgtagg cgcgggcggg cattttgagg
    17281 gcggtgtagc ccagcatttc ccatatgccg taactgcctt tgccgaggtt gccggtttcg
    17341 tacaggattt caaaaaagct gtacaaagcg aggaaggcaa ggagcgcgta aaccgccata
    17401 accgccattt gacggatgat gtaacgtgaa atcaggttca ttttccgcct ttcaatgtca
    17461 gacttttgcc aaccgcctgc cagaagggct ggctgggcat gctgcggacg cgcagaagta
    17521 cgattgcgat gacgaacatg atgatgtgca taggcagcag tccgagccaa aaatggattt
    17581 tgccgtcttc cacggcttca aaaagcaggg tcagcccgtt ttggtaaatt aaaaacaaac
    17641 cgatggcaat caagatattg taggtatgtc cgctgcgcgg gttgaaatag gaaagcggca
    17701 cggcaagcag gcagagtagg aggacgctga cggtcagcga gatgcgccac atcaattccg
    17761 cctgatgttg cgggttgctg ctgccaatca gttgggcggt cggaatggta cggcggtggg
    17821 aaacggggtc gatgagtttt ggcgtggtgc tgataatcag gttgagtttt tggaaggaaa
    17881 cctgattgta gtcggcgcgt ccgggcgtgc cgctgtaacg gtagccgtgg cgcaattcga
    17941 gcgtgcgttt gttgtcgttc agcgagaagt taccttcttt ggcgaagatg atgttgtcgc
    18001 cgccgttttt gtcctgttcg cgcaggaaca ggtttttcat gatgccggat tcggtatcga
    18061 aggtttcgac aaaataaacc ctgccgttgc gcttgcccaa actgttgaac ccgcctgcct
    18121 ccaccaaaga caattcctgc ttctgcttca ggatttcagc gtattcgcgg ctgcgtagct
    18181 ctgcccacgg tatcacccaa agctgcatga cggcaaccaa aacggcaaac ggcacggcaa
    18241 actgcatcac cgggcgtatc cattgtttca atgccaatcc gcaggatagc cagaccgaca
    18301 tttcgctgtc gcgccagtag cgggtcaaca cggtcaacgt actgataaat gcggtcaaca
    18361 ccagcaaaag cggcgtcata ccgatgaccc agaagccgac caatgccaac acggcatcga
    18421 tggcgacacg cccgtcggcg gcacggccga gcaggttgat tgcctgcgtg gagaccaata
    18481 ccgccaagag gacgacgaaa atgccgacgg cggtaaaaga gagttctttg atgaggtttc
    18541 tttgataaat catagaatcg cggtcagggg gcggtaagcg gttcggcgca ggcggattgt
    18601 ccgcgcaagg tttcagacgg tgcttcaaat ggggtacaat cgtgccagcc gtcttcagac
    18661 ggtcttttga gcgaaaggcc gtccgaaaac taccgacaac cgtctcaatc aggagaataa
    18721 acgtggaatt tagcacaaaa accgaaatct tgcaggaaca gcaggcaggc gcacagttat
    18781 ttgtctgcac cgaagaggcg caattaaacc atccgaccgc ccttgccctt ttgtcttcgc
    18841 ttgaagaagg tcaaaatttt gccgacacaa aaatcccgac aggcaacggt ttgcaggctg
    18901 ttgccgtatg ctgtctgaaa agcaccggcc gcgccgcatt gaacaaggcc gccgccgaag
    18961 ccgccaaatg ggcgcaaaat caggaaacgg tcaatgtgga catccatgcc ttcgatgaag
    19021 cgcaagccgc cgccgtagcc gaagcgtttg cgattgcgtt cggcaacgcc gcctaccgtt
    19081 tcgaccgcta caaaaaagaa gccaagcccg ccaaatttga aaccgccgtg ttccacagcg
    19141 cacacgaagc cgtcgttaaa gaagccttgc gcgtcgccga agcgcaagtt tacggacaaa
    19201 gcctctgccg cgacttgggc aacgccgcgc ccaacgaatg cacgcccgaa tttttagcgc
    19261 gcaccgccaa agccgaagcc gaaaaactgg gcgcgcacgc caaaatcatc gaaaaagact
    19321 acatcaaaga aaacatgggt tcgttttggt cggtcgccaa aggcagcgcg gaagccccct
    19381 atttggtcga actgagctat ttcggtgcag ccgacaaaga agccgcgcct gtggtattgg
    19441 tcggcaaagg cattaccttc gacaccggcg gcatctccct caagcccggc ctgaacatgg
    19501 acgaaatgaa gtttgatatg tgcggcgcgg caaccgtcat cagcaccttc tgcgccgccg
    19561 tcaaactgca actgccgatt aacctgattg ccgtcgtcgc cacttgtgaa aatatgcctt
    19621 ccggcgcggc aaacaaaccg ggcgatgtcg tgaaaagtat gaaaggtctg accatcgaag
    19681 tgttgaacac cgatgccgaa ggccgtctga ttttgtgcga cgcgctcact tacgccgagc
    19741 agttcaaacc caaagccgtc atcgacgtcg ccaccctgac cggcgcgtgt attgttgcct
    19801 tggggcacga tgtcagcggc gtgatgggca acaatcagga tttgatcgac agcctgcttg
    19861 ccgcttccta caacgtggac gacaaagcat ggcaactgcc gctctttgag acctacaaag
    19921 accaactcaa atccaacttt gccgacattc caaacatcgg cacgcccggc gcgggcacga
    19981 ttaccgccgc aacattcctg tcttacttca ccgaaggcta cccgtgggcg caccttgaca
    20041 tcgcgggtac ggcgtggaaa tccggagcgg aaaaaggtgc gactggccgt cccgttccct
    20101 tgctgctgaa ctatctgcgg aatctttaat tttgccacaa tgccgtctga agcctttcag
    20161 acggcatcgt cctgccaaat gctcaaacaa tatgccgaaa gccacctttt acacgcacgt
    20221 tgcccaagtt ccgattttca cctgccgcct gattgcccgt gcgattcggg acggcggcag
    20281 gatactggtg tggtccgatt cgttcgggcg gcttcaggaa ttggacaaaa tgctttggca
    20341 atacgaagcc gagagtttca ttccgcacga aatttgggaa atggaagaag ccatgccgtc
    20401 tgaaacatcc gtcctgcttg cttgcggcgg caatccgccc cgaattcccg aaggcatgac
    20461 tgttttgaac ctgtccgacg gtttttggaa caccgctccg gtcttgcccg cgcgcgtttt
    20521 ggaaatcgtc ggcaacagct tggaagatct cgccgacgcg cgcgaacgct ttaccgccta
    20581 ccgccgaagc ggttttgcca tcgaacatca cggcatggag ggcaaggcat aaaatgccgt
    20641 ctgaaatgcc gcatttacaa aatttaaaca ggcttgattc tatttgatta aaatttcctt
    20701 atcggttgaa ccccgccact tggacatctg tccctcgggg cggtagaatc aaaacttatt
    20761 ttggaaggtt caatcccttc caaaacaggg caacacacga ttgacgcttt atgtgtcatc
    20821 ctgtgtgttg aaacattcaa actcggctat aatcccgttt ttccgacctt atcgacagcg
    20881 aagatccatc atgaacacca ttttcaaaat cagcgcactg accctttccg ccgctttggc
    20941 actttccgcc tgtggcaaaa aagaagccgc atccgaacct gccgccgctt ctgccgcgca
    21001 gggcgacacc tcttcgatcg gcagcacgat gcagcaggca agctatgcga tgggcgtgga
    21061 catcggacgc tccctgaagc aaatgaagga acagggtgcg gaaatcgatt tgaaagtctt
    21121 taccgaagcc atgcaggcag tgtatgacgg caaagaaatc aaaatgaccg aagagcaggc
    21181 tcaggaagtc atgatgaaat tccttcagga acaacaggct aaagccgtag aaaaacacaa
    21241 ggcggaagcg aaggccaata aagaaaaagg cgaagccttt ctgaaagaaa atgccgccaa
    21301 agacggcgtg aagaccactg cttccggcct gcaatacaaa atcaccaaac agggcgaagg
    21361 caaacagccg accaaagacg acatcgttac cgtggaatac gaaggccgcc tgattgacgg
    21421 tacggtattc gacagcagca aagccaacgg cggcccggtc accttccctt tgagccaagt
    21481 gattccgggt tggaccgaag gcgtacagct tctgaaagaa ggcggcgaag ccacgttcta
    21541 catcccgtcc aaccttgcct accgcgaaca gggtgcgggc gacaaaatcg gcccgaacgc
    21601 cactttggta tttgatgtga aactggtcaa aatcggcgca cccgaaaacg cgcccgccaa
    21661 gcagccggct caagtcgaca tcaaaaaagt aaattaagtc cgaatccatg cccgaaacag
    21721 gttttcgggc atttttacgg caaatgccgt ctgaaaccac caaacagcgg ttcagatggc
    21781 atccctttca aaagcgtacc attatgaaaa acatcgtcat cctgatttcc ggacgcggca
    21841 gcaatatgca ggcaatcgtc aatgccgcca ttcccaacgt ccgcattgcc gccgtgttga
    21901 gcaacagaga aacggctgcc ggtttgcaat gggcggccga acgcggcatc ccgaccgata
    21961 gcctgaatca taaaaacttt gaatcacgac ttgccttcga tacggccatg atggaaaaaa
    22021 tcgacgcata tcaacccgac ttggtggttt tggcaggttt tatgcggatt ctgacccccg
    22081 agttttgcgc ccgttacgaa ggcaggctga tgaacattca cccgtccatc ctgccctcgt
    22141 ttaccggact tcatacgcac gaacgcgctt tggaagcggg ttgccgcgtt gcaggctgta
    22201 cgattcactt tgttactgcc gaactggatt gcggcccgat tgtatcgcaa gggattgtgc
    22261 cgatacttga cggcgatacg gcagacgata ttgccgcacg ggttttggct gtcgagcata
    22321 aactttatcc gaaagccgtt gccgattttg ccgccggccg cctgattatc gagggaaacc
    22381 gcgtcagaaa ttcggaaaac gccgatgccg cccgtttcct gacggcgtaa accgggcggg
    22441 agcaaatgat gaagactttt aaaaatatat tttccgccgc cattttgtcc gccgccctgc
    22501 cgtgcgcgta tgcggcaggg ctgccccaat ccgccgtgct gcactattcc ggcagctacg
    22561 gcattcccgc cacgatgaca tttgaacgca gcggcaatgc ttacaaaatc gtttcgacga
    22621 ttaaagtgcc gctatacaat atccgtttcg agtccggcgg tacggttgtc ggcaataccc
    22681 tgcaccctac ctactataga gacatacgca ggggcaaact gtatgcggaa gccaaattcg
    22741 ccgacggcag cgtaacctac ggcaaagcgg gcgagagcaa aaccgagcaa agccccaagg
    22801 ctatggattt gttcacgctt gcctggcagt tggcggcaaa tgacgcgaaa ctcccctcgg
    22861 ggctgaaaat caccaacggc aaaaaacttt attccgtcgg cggtttgaat aaggcgggta
    22921 caggaaaata cagcataggc ggcgtggaaa ccgaagtcgt caaatatcgg gtgcggcgcg
    22981 gcgacgatac ggtaatgtat ttcttcgcac cgtccctgaa caatattccg gcacaaatcg
    23041 gctataccga cgacggcaaa acctatacgc tgaaactcaa atcggtgcag atcaacggcc
    23101 agtccgccaa accgtaaatc ggttttcaag tgtttttttg ccgttttgtc catatttctg
    23161 cataagccgc ttgaaattgt ttacaataac gccatatgca aagcggtttt aacgctattt
    23221 tcaggaatga caccatgcag gttacatcaa aatggataga cggaatgtgc tttgtcggca
    23281 cgacggaagg cgggcacagc gtcgttatgg agggttcggc ggcagaaggt gcggccaagc
    23341 gcgggcccag ccctttggaa atgctgctgt tgggcgtggc gggctgttcg agcatcgatg
    23401 tggtgatgat tgccgaaaaa cagcgtcaga aagtgactga ctgccgtgcg acggttacgg
    23461 cgaaacgggc ggacgatgcg ccgcgcgtgt ttaccgaaat ccacatccat ttcaaagtat
    23521 tcgggcatga tttgaaagaa tcggccattg agcgcgccgt tcagatgtct gccgaaaaat
    23581 actgttcggc ttcgattatg ttgggcaaag cggcaaagat tacccacagt tttgaaattg
    23641 ccggggcaga taaataggca tcgggcggca ttatggggaa acggctgatg ccgtctgaag
    23701 ggaaaggtgc ggcaggcaat ccggaggcgc ataaaaaagc agatatattc ggactgcacc
    23761 tcccgaatat atctgcctgc tgtttcctct ttattcagcc tttataatac ttggacttgt
    23821 cggggtattg tgcagacttg attccggatt gtcaacaatt ttcggtcaaa ttttaaatgc
    23881 cgcgttttaa aatgataccc gcctgcttct gcgggcgggc gggaggcgag aatcccgtta
    23941 ttttaagcca tatattaatt tattgaatta tagtggatta acaaaaacca gtacggcgtt
    24001 gcctcgcctt ggctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
    24061 tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
    24121 ataaaataaa tttgtgtaat actttgattt ccccgacacg atttgaagca cagccgcctt
    24181 aaaacatctt aaaaaatttg aaagcgtaaa aatacggaaa acatcatgaa ctttgaaaac
    24241 gacgacatta tccatgcgcc gaccacgtct tccttgattc tcgaagagcg gcacgattcg
    24301 gagctgttcc gtgtttacgc cctgattttg gacggcatta ccgatcaggt gctgctgccc
    24361 ggcaaaaagc tgaccgaatc cgaactttgc cgccagatgg tgtgttcgcg caacaccgtc
    24421 cgcggcgcgt tgtcgctttt ggcgcacgac aagattgtcg atttgcaacc caacaggggc
    24481 gcgttcgtcc acgttcccga tttgaaagaa atgcaggatg tgttcaatgc gcgcatcgaa
    24541 atggagacga tgattttgaa tatcctcgca gatttgccgg atttggaaac gcgcctcaag
    24601 ccgctttatg cgatgatacg gcgcgaagaa gaggcctccg gcaggggaga ccgcgttggc
    24661 tggaaccgcc tgtccaatgc cttccacgtc gaactggcgc gcctggtggg caacgatgtg
    24721 ctgttcgaca ttatgaacac gctgtgcgcg cggtcttccc tgattgtcgc cgtggcgggc
    24781 gtgcatcgcg aggaaaaaca cgccatcaat acgcatacgc attccgaaca ccgcgaaatc
    24841 ctcgacctgc tgctggcggg caggcgcaac cgcgtggtca aaatcctgcg ccgccatttg
    24901 ggcaactgta tggagcgttt ggaaaagact ttggaagatt gaatgccgga gcggataagc
    24961 cgcctgaacc ttcaggcggt tttttaatgg cgaacctgat gccgtctgaa atatggatgc
    25021 gggtatctgc aattttcaga cggcaatttt taaaccgcat atatcatgcg gcaataaagg
    25081 aggtcaggag atggacagcc tgatgacgtt gctttcggta ttgataccga tgtttgccgg
    25141 attttttatc cgtgtgccca agccttacct gcccgctttg gataaggtgc tatcggtctt
    25201 ggtgtatgct gtgctgctgc tgatcggcgt ctcgttgtcg cgcgtggagg atttgggttc
    25261 gcggttggac gatatggcgt tgacggttct gtggctgttt gtttgtacgg tcgggacgaa
    25321 cctgcttgct ttggcagtgt tgggaaagtt attcccgtgg cggataaagg ggaaagggaa
    25381 gggcgtttcg gtcggtgtgt cgggcagtgt ggggcagctc ggatgcgtgc tgctcggatt
    25441 tgcatccggc aaactgatgc gcgatatttg gatgccgtct gaaaacgcgg gtatgtattg
    25501 tctgatgctg ctggtgctcc tcatcggcgt acagctcaaa agcagcggcg tatcgttgcg
    25561 gcaggttttg gtcaaccgca ggggtattcg gttgtcggtc tagtttatgc tttcatctct
    25621 ttcaggcggg ctgctgtttg ccgcatcggc agacggtgtg tcgtgggtga aaggtttggc
    25681 gatggcttcc ggcttcggtt ggtattccct ctcgggtttg gtgatgaccg aggcttacgg
    25741 cgcggtatgg ggcagtatcg cgcttttgaa cgatttggca cgagagctgt tcgcgctggc
    25801 atttattccg ctgctgatga agcgttttcc cgatgcggca gtgggggtcg gcggcgcgac
    25861 cagtatggat ttcacattgc ccgtgattcg gggtgcgggc ggcttggaag ccgtaccggt
    25921 agcggtcagc ttcggcgtgg tggtcaatat cgccgctccg tttctgatgg tggtgttttc
    25981 cgctttgggc tgaacgcggt aaaatcggca tcccgatgca aggaggcaga aaacgatgaa
    26041 accgaaaatc caaaggcacg gagagatttt aagccttgtc cgccggcatc agtttatgtc
    26101 ggtggacgag cttgccgccg cattggacgt taccccgcag acgatacgcc gcgacatccg
    26161 cgaattggag gatgctggca gcctgaaacg ccaccacggc ggcgcatcct cgggcggaaa
    26221 cttgccggag gggctgcctg ccagccgcca aattcaatgt caaaacgaaa aaaacgccat
    26281 tgcccggctg attgcggaac atattcccga cgagtcatcg ctgtttgtca gtatcggcac
    26341 gacggtggaa gccgtggcat cggagctggt gagacggcgc ggcagcctgc ggattattac
    26401 caacaatatc cacgtcgcct ccatcgtttc ggcgcggacg gattacacgg tcatcatcac
    26461 ttccggagtc gtccgccctt tggacggcgg cattaccggc gtggcaaccg tcgattttat
    26521 caaccagttc aaagtcgatt atgccgtgat gagtacgcac ggcgtggaaa gcgacggttc
    26581 gcttttggac gacgactaca aagaagtcag cgtgatgcag gcgatgattg ccaacgcgcg
    26641 cgtccgcttc ctcggcgtgg accacagtaa attccgcagc aacgcgctgg tcaggctcgg
    26701 cgacattacg gcgtttgaca aagtatttac cgaccggctg cccgataccg cgatgcagaa
    26761 gatgctgaaa gaggcggggg tggaatgcct gattgccgat gccgtctgaa cgctatgtca
    26821 aagcgcgcaa gtcgggtaca ataaacacat catcaaaccg cttcagacgg cataaggaac
    26881 cgccccaatg ccgtctgaag ccatctgttc aaagaaaact atgctcaata aagaccaatt
    26941 tgcggacaac cattttatcc gcaccttcat cgaagaagac ctcaaaagcg gcaaacacga
    27001 aaccatccaa acccgttttc cgccggagcc caacggctac ctgcacatcg gacacgccaa
    27061 atccatctgc ctgaacttcg gtttggcgta tatttacgac ggcttgtgca acctgcgttt
    27121 tgacgatacc aaccccgaaa aagaaaacga cgaatacgtc aacgccatca aagaagacgt
    27181 tgagtggctc ggtttccatt gggcgggcga gccgcgtttc gcttccaact atttcgacca
    27241 gctttatgac tacgccgtcg gtttggttaa agacggcaaa gcgtatgtcg atgatttgac
    27301 gccagaagaa atgcgcgaat accgcggtac gctgaccgaa gcaggcaaaa acagccctta
    27361 tcgcgaccgc agtatcgaag aaaacctcga cctgttcacg cgcatgaaaa acggtgagtt
    27421 ccccgacggc agcaaaaccc tgcgcctgaa aatcgacatg gcatccggca acatcaatat
    27481 gcgcgatccg gtcatctacc gcatccgccg cgcccatcac cacaacaccg gcgacaaatg
    27541 gtgcatctat ccgatgtacg actacacgca ttgtatttcc gatgccatcg aaggcatcac
    27601 gcattccttg tgtacgctcg aatttgaagc ccatcgtccg ctgtatgact gggtgttgga
    27661 caacatcccc gcgccgcacg ccacacgtcc gcgccaatac gagttttcac gcttggagct
    27721 tttgtacacc attacctcca aacggaaatt gaatcagttg gttgtggaaa aacacgtttc
    27781 cggctgggac gatccgcgta tgccgaccat ttccggtatg cgccgacgcg gctacacgcc
    27841 cgaagggctg cgcctgtttg ccaaacgcgc cggtatttcc aaatctgaaa acatcgtcga
    27901 tatgagcgtg ttggaaggcg cgattcgcga agagttggaa aattccgccc cacgcctgat
    27961 ggcggtgttg aacccgctca aagtgaccct gaccaacttt gaaaccggca gaacccaaag
    28021 ccgccgtgcc gcgttccatc cgaaccacga ggaaatgggc gagcgcgaag tacctatttc
    28081 acaaaccatc tatattgaag ccgacgactt tgccgaaaat ccgcccaaag gcttcaaacg
    28141 cctgattccc ggcggcgaag tacgcttgcg ccacggctat gtcatcaagt gcgatgaagt
    28201 cgtcaaagac gaagcaggca atgtggttga actcaaatgc agcatcgacc acgacacctt
    28261 gggcaaaaat ccagaaggcc gtaaagttaa aggcgtgatt cattgggttt ccgccgaaca
    28321 cgcagccgaa atcaaagtcc gcctgtacga ccgcctcttt accgtcgagc gtcccgatgc
    28381 cgtgcgcggc gaagatggcg aatacctgcc gtttgccgat ttcctcaatc cggaatccgt
    28441 taaggaaatc actgcttacg ccgaacctgc cgcgaaagat ttgccggcag aaagccgttg
    28501 gcagttcgag cgcatcggct attttgtgac cgaccgcaaa gaccacggca aagacacgcc
    28561 ggtgttcaac cgcacggtca cgctgaaaga ttcttggcag cctaagtaaa accccatccc
    28621 tgccgtctga atattgttcg ggcggcattt cccctttgcc cgcgaaatgc ggcacattcg
    28681 gcacactgga aaggaaatat gatgaaagtc ctctttatcg ccgacccgat ggcaagtttc
    28741 aaaacctaca aagacaccac ctacgccatg atgcgcgaga tggcaaaacg cggttggcgg
    28801 ctgtttcata ccttgagcag cggattgtcg gttaaaaacg gtctggtggt tgctgatgcg
    28861 tctgcttttg agtttctcgg cgcacaagac gaccacgaca aaaactggtt tgccgccgcc
    28921 gacaaagttc agacggcatt gaaagcattt gatgccgtga ttatgcgtac cgatccgccg
    28981 ttcgatatgc aatacctcta cgccacacag cttctgacgc tggcggagca gcagggcgcg
    29041 aaggtcttta acagcggaca ggcgatgcgt gatttcaatg aaaaactggc gattttgaac
    29101 ttcagccgct ttaccgcgcc cacgctggta acgacccgtt ccgccgatgt ccgcgcattt
    29161 ttgaaagaac acggcgacat catcattaaa ccgctcgacg gcatgggcgg tatgggcatc
    29221 ttccgcctga ccgaaaaaga ccccaacatc ggcagcatcc tcgaaaccct gatgcggctt
    29281 gattcccgca ccattatggc gcaacgctac attcccgaaa tcgtacacgg tgacaaacgc
    29341 atcctgatta tcggcggcga agtcgtctcc tatgctttgg cgcgtatccc gcaaaacggc
    29401 gaaacacgcg gcaaccttgc ggcaggcgga cgcggcgtgg cgcaggaatt ggacggacgc
    29461 gaccgcgaaa ttgccgaaac gctcgccccc gaactcaaac ggcgcggcat cctgctggcc
    29521 ggtttggacg ttatcggcag caacctgacc gaagtcaacg taaccagccc gaccggattc
    29581 caagaaatta tgaaacaaaa aggtttcgac gtggcggcaa tgtttgccga tgccgttgcc
    29641 gcgtggtcgg tacgttaaac cgatgccgtt tgaaaggctt ttgcttcaca accgtttggt
    29701 ttgttcgggc aggcagggtt ttccctcagc cggcagggat gcggttaatg ccgtacggac
    29761 accccgccgc cccgttttca gacggtatat atagtggatt aacaaaaatc aggacaaggc
    29821 ggagaagctg cagacagtac aaatagtacg gaaccgattc actcggtgct tcagcacctt
    29881 agagaatcgt tctctttgag ctaaggcggg gcaacgccgt acaggttttt gttaatccac
    29941 tatattgaga acattttgaa aggataccga tggaaccttc ctcctacgcg gcagaaaaaa
    30001 aaggcaaagg cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg
    30061 ccgccgccta ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg
    30121 tgtgcgcggc atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt
    30181 cttttgtgtc ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata
    30241 cttcgaccga aaaacacgag ctggccaaac gcgccaaaga cgcaggttct gccgcacaat
    30301 tgttcgcgat gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt
    30361 gcagcaggat ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatctac
    30421 atcaaatttc caggcagtat ttgtatgtat agtggattaa caaaaatcag gacaaggcga
    30481 cgaagccgca gacagtacaa atagtacgga accgatccac ttggtgcttc agcaccttag
    30541 agaatcgttc tctttgagct aaggcgaggc aacgctgtac tggtttttgt taatccgcta
    30601 taagattaag cacattcccc gtctgatatt ataaggagca gaaagatgaa aaaattattg
    30661 gcggccgtga tgatggcagg tttggcaggc gcggtttccg ccgccggagt ccacgttgag
    30721 gacggctggg cgcgcaccac cgtcgaaggt atgaaaatag gcggcgcgtt catgaaaatc
    30781 cacaacgacg aagccaaaca agactttttg ctcggcggaa gcagccccgt tgccgaccgc
    30841 gtcgaagtgc atacccacat caacgacaac ggcgtgatgc ggatgcgcga agtcgaaggc
    30901 ggcgtgcctt tggaagcgaa atccgttacc gaactcaaac ccggcagcta tcatgtgatg
    30961 tttatgggtt tgaaaaaaca attaaaagag ggcgataaaa ttcccgttac cctgaaattt
    31021 aaaaacgcca aagcgcaaac cgtccaactg gaagtcaaaa tcgcgccgat gccggcaatg
    31081 aaccacggtc atcaccacgg cgaagcgcat cagcactaat ctgctgaaaa tgtttgaaat
    31141 gccgtctgaa aaagcccggg ctttcagacg gcatttttat gcccgccttt aaaatgtgtt
    31201 aaaatccgcc tttaaaaacc gccgtttcca accatattct acgcatgaat acgacagcaa
    31261 acccttccaa tatcatcgtc gggctctccg gcggtgtcga ttcttccgta accgccgccc
    31321 tgctcaagca gcagggttat caagtgcgcg gtgtgttcat gcagaactgg gaagacgacg
    31381 acaacgacga atattgcagc atcaaacagg attcgttcga tgccatcgcc gttgccgata
    31441 ttatcggcat cgatatcgac atcgttaatt tcgccgcgca atataaagac aaagtctttg
    31501 cttattttct tcaggaatac agtgcggggc gcacgccgaa tccggatgtg ttgtgcaacg
    31561 ccgaaatcaa attcaaatgc tttttggact acgccgtagg gcagggcgcg gataccatcg
    31621 ccaccggcca ctatgcgcgc aaagaagttc gcaacggcgt gcattacctg ctcaaaggtt
    31681 tggatcgaaa caaagaccaa agctatttcc tctaccgcct caagcctttc caactcgaac
    31741 gcgcgatttt tccgttgggc ggtttggaaa aacccgaagt gcgccgcctt gccgccgagt
    31801 tcaaactgcc gaccgccgcc aaaaaagaca gcacgggcat ctgcttcatc ggtgaacgcc
    31861 cgtttcgcga gtttctgcaa aaatacctgc cgaccgacaa cggtaaaatg gttacgcccg
    31921 aagggaaaac cgtcggcgaa cacgtcgggc tgatgttcta cacgctcgga cagcgcaaag
    31981 gtttgggcat cggcggcgcg ggcgaaccgt ggtttgttgc ggctaaagat ttgacgaaaa
    32041 acgaactgat tgtcgtgcaa ggacacgacc atccgctgct ctatacccgc agccttgtga
    32101 tgaacgattt gagtttcacg cttcccgaac gcccgaaggc aggacgctat acctgcaaaa
    32161 cgcgttaccg tatggcggac gcgccttgtg agttgcgcta tttggatgaa gaaaccgccg
    32221 agctggtgtt tgacgaaccg caatgggcgg ttacgccggg ccagtctgcc gtgctgtacg
    32281 acggcgacat ctgtttgggc ggcggcatca tccaaacgac cgacaaaccc gtcatcatca
    32341 cacgataaag gtaatgccgt ctgaaacggt tttcagacgg cattgttccg ctcaattcca
    32401 ctttaaagac cgatacctat ggaaaaaatc tggttagaca gctacgagaa gggcgtcagt
    32461 gccgaaatcg acatcacgca atacaattcc gtcagcgacg tattccgcca aagcgtggaa
    32521 aaatttgccg accaacctgc ttttcaaaac atgggtaaga cgctgactta tgccgaaacc
    32581 ggcaaactgg cgaccgattt cgcctcttat ctgcaaaacg tcctcaagct gccgcgcggc
    32641 gaacgtgttg ccattatgat gccgaacgta ttgcaatacc cgattgccct tttcggcatt
    32701 ttgcaggcag gtttggtagc ggtgaacacc aatccgctct ataccccgcg cgagctggag
    32761 catcagttga aagacagcgg cgcgaccgcc atcatcgttt tggaaaattt cgccaacacg
    32821 ctggagctgg tgctgccgcg cacgcagatc aaacacgtca tcgtagcctc cgtcggcgaa
    32881 atgttcgggc tgcttaaagg ttcgctgatc aatttcgtca tccggaaaat caagaaaatg
    32941 gtgcccgaat accgtattcg ggaaaccatt tcctttcaga cggcattgaa agagggggcg
    33001 aagcacgttt tccaacctgt cgcattaaac cgcgaagata ccgcgctgtt gcaatacacg
    33061 ggcggcacga caggtgttgc caaaggggcg gtgctgagcc acggcaacat ctgcgccaat
    33121 atgcttcagg caaaagaatg gattaaaaac cagttgcgtg agggaaaaga aaccgttatc
    33181 gccgccttgc cgctgtacca tatttttgcc ttaaccgtga atctgatgat ttttgccaat
    33241 gccggctcga aaattgtcct gattaccaac ccgcgcgata tgaaaggctt tatcggagaa
    33301 ctgaaaaaac agcgggttaa tgtatttatc ggcgtgaaca cgctgtttaa cgcgatggtt
    33361 aaccgtcctg attttgccga agtcgatttt tcaggattgc ggctgacttt gggcggcggt
    33421 atggcgaccc aaaaagccgt tgccgaaaaa tggaaaaaaa tcaccgacac gcccatcgtc
    33481 gaagcctacg gtctgaccga agccagcccc ggcgtgtgct gcaacccctt aaacatcgaa
    33541 tcatacagcg gcagcatcgg tttgcccgtc tcgtccaccg aagtcgaact gcgcgacgca
    33601 aacggcaaag aagtccccgt cgggcagccg ggcgaattgt gggtgcgcgg cccgcaagtc
    33661 atgaaaggct attggaaccg tcccgaagaa accgctaaag ccatagacgc gcgcggtttc
    33721 ttggaaaccg gcgatattgc cgtcatggac gaaaaaggct ggttgaagct ggttgaccgt
    33781 aagaaagacc tcattgttgt ttctggtttt aatgtttatc cgaacgaaat cgaggaagtc
    33841 atcgcgcatc acggcaaagt gatggaagtc gcctgtatcg gcgttcccga cgaaaaaacc
    33901 ggagaagctc tcaaagtgtt tgtcgtcaga aaagatccgt ctttgacgaa agaagaactt
    33961 atcgcctttt gccgcaccga attaaccgca tataaagtac cgaaaaatat cgaattccgc
    34021 gacgagttgc ccaagtccaa cgtcggcaaa atcctgcgcc gcgagttgcg gcaaagtact
    34081 gggaaataaa gaaaagatgc cgtctgaaaa cagccgtcca ccgttcagac ggcatccgtc
    34141 cgtttgccag aaccgcgcgc ttcaagttaa aatcgcacat tccaacatgg gtattccatc
    34201 atgaccaaat tcattttcgt aaccggcggc gttgtctcct cactgggtaa aggtatcgcc
    34261 gccgcttcta ttgccgctat cctcgaatcg cgcggcttga acgttaccat gctcaagctt
    34321 gatccttata tcaacgtcga tcccggcacg atgagcccgt ttcaacacgg cgaagtgttc
    34381 gtaaccgacg acggcgcgga aaccgacctc gacttaggac actacgaacg gtttatcgat
    34441 tccacgatga cccgccgcaa cagcttcagc acggggcagg tgtacgaaaa cgtcatcgcc
    34501 aaagaacgac ggggcgacta cctcggcggc acggttcaag tcatcccgca cattaccgac
    34561 gaaatcaaac gccgcatcca cgaaggcgcg gcaggttacg atgtggcgat tgtcgaaatc
    34621 ggcggcacgg tcggcgacat cgaatcgctg ccgtttttgg aagccatccg ccagatgcga
    34681 agccagttgg gacgcaacaa caccctgttc gcccacttga gctacgtccc ctacatcgcc
    34741 gctgcaggcg aaatcaaaac caagccgacc cagcacaccg ttaaagaaat gttgagcatc
    34801 ggtttgcaac ccgacatcct gatttgccgt atggacagga caatgcctgc ggacgaacgc
    34861 cgcaaaatcg ccttgttctg caacgtggaa gaacgcgcga ttgtcggcag ctacgatgtg
    34921 gacagcatct acgaatgccc cgaaatgctg cacgaccaag gcatcgacaa catcattacc
    34981 gagcagttgc agcttaatgt gcagcaggcg gatttgaccg cgtggaaaaa aatcgtccac
    35041 gccatccaaa acccgaaaca caccgtcaaa atcgccatgg tcggcaaata cgtcgatttg
    35101 accgaatcct acaaatcact gattgaagcc ttgaaacacg caggtgttca caccgaaacc
    35161 gacgtacaaa ttacctttgt tgacagcgaa agcatcgaga aaaacaacgg cgacgtttct
    35221 atgctcaaag atatggacgc catcctcgtt cccggcggtt tcggttcgcg cggcgtggaa
    35281 ggcaaaatcg ccgctgtgcg ctatgcccgc gaaaacaacg tgccatactt gggcatctgc
    35341 ctcggtatgc agattgcgct gatcgaatac gcccgcgacg tggcaggttt gaaaggtgcg
    35401 aattccaccg agttcgacct caaatgcgcc gcccccgtcg ttgccctgat tgacgaatgg
    35461 caaactgccg acggcagcgt cgaaacccgc gacgaatccg ccgatttggg cggcaccatg
    35521 cgtttgggcg cgcaagaagt cgaattgaaa acaggcagcc tcgccgccaa aatctacggc
    35581 agcggacaca tccgcgaacg ccaccgccac cgctacgaag tcaacaacaa ctatgttcct
    35641 acgctggaac aggcaggttt ggtcatcggc ggcgtatccg ccggacgcga acgcttggtc
    35701 gaaaccatcg aactgccgaa ccatccttgg ttcttcgcct gccagttcca ccccgagttt
    35761 acctccaatc cgcgcaaagg gcatcctttg ttcaccgcgt ttgtcaaagc tgcgttgaac
    35821 aataaaaaag cctgataaag cgttacttga tgatcaaaat gccgtctgaa agcctgaatg
    35881 aagactttca gacggcattt tcgcaaatcg gggattataa aagccatcaa tttaattttt
    35941 atggttttat ctaatattta ccaactgaat attgttaagc ggggcgtgaa actcaagttg
    36001 agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt aggagcacct
    36061 tcttccg
//
LOCUS       DAXIHS010000011        34899 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXIHS010000011 DAXIHS010000000
VERSION     DAXIHS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.011"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(186..260)
                     /locus_tag="ACFOF2_000446"
     tRNA            complement(186..260)
                     /locus_tag="ACFOF2_000446"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Glu,seq:ttc)
     gene            complement(278..354)
                     /locus_tag="ACFOF2_000447"
     tRNA            complement(278..354)
                     /locus_tag="ACFOF2_000447"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Arg,seq:acg)
     gene            complement(464..2137)
                     /gene="recN"
                     /locus_tag="ACFOF2_000448"
     CDS             complement(464..2137)
                     /gene="recN"
                     /locus_tag="ACFOF2_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC7106824.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2321..2923)
                     /locus_tag="ACFOF2_000449"
     CDS             complement(2321..2923)
                     /locus_tag="ACFOF2_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC7106825.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(3024..3965)
                     /gene="ylqF"
                     /locus_tag="ACFOF2_000450"
     CDS             complement(3024..3965)
                     /gene="ylqF"
                     /locus_tag="ACFOF2_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC7106826.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGKKPSEA"
     gene            complement(4046..4900)
                     /gene="rapZ"
                     /locus_tag="ACFOF2_000451"
     CDS             complement(4046..4900)
                     /gene="rapZ"
                     /locus_tag="ACFOF2_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC7106827.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4881..5843)
                     /gene="hprK"
                     /locus_tag="ACFOF2_000452"
     CDS             complement(4881..5843)
                     /gene="hprK"
                     /locus_tag="ACFOF2_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC7106828.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5845..6294)
                     /gene="ptsN"
                     /locus_tag="ACFOF2_000453"
     CDS             complement(5845..6294)
                     /gene="ptsN"
                     /locus_tag="ACFOF2_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC7106829.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6479..7369)
                     /gene="ubiA"
                     /locus_tag="ACFOF2_000454"
     CDS             complement(6479..7369)
                     /gene="ubiA"
                     /locus_tag="ACFOF2_000454"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC7106830.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7399..7875)
                     /locus_tag="ACFOF2_000455"
     CDS             complement(7399..7875)
                     /locus_tag="ACFOF2_000455"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC7106831.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7878..8261)
                     /locus_tag="ACFOF2_000456"
     CDS             complement(7878..8261)
                     /locus_tag="ACFOF2_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106832.1"
                     /translation="MITRCFKVVIIVFIMVMTTSCNPCFFATSSCNYPFDDMTIRANN
                     DIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLPDEKGNYQYIIKKYYDNKYNDN
                     AVKSTNCIGDNARCMLNNKGYSTKW"
     gene            complement(8332..8775)
                     /locus_tag="ACFOF2_000457"
     CDS             complement(8332..8775)
                     /locus_tag="ACFOF2_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106833.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            complement(8769..9155)
                     /locus_tag="ACFOF2_000458"
     CDS             complement(8769..9155)
                     /locus_tag="ACFOF2_000458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106834.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            complement(9179..9409)
                     /locus_tag="ACFOF2_000459"
     CDS             complement(9179..9409)
                     /locus_tag="ACFOF2_000459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106835.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            complement(9376..10023)
                     /gene="bioD"
                     /locus_tag="ACFOF2_000460"
     CDS             complement(9376..10023)
                     /gene="bioD"
                     /locus_tag="ACFOF2_000460"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC7106836.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(10020..11321)
                     /gene="bioA"
                     /locus_tag="ACFOF2_000461"
     CDS             complement(10020..11321)
                     /gene="bioA"
                     /locus_tag="ACFOF2_000461"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC7106837.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(11535..11774)
                     /locus_tag="ACFOF2_000462"
     CDS             complement(11535..11774)
                     /locus_tag="ACFOF2_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106838.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            complement(12034..12519)
                     /locus_tag="ACFOF2_000463"
     CDS             complement(12034..12519)
                     /locus_tag="ACFOF2_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC7106839.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(12849..13157)
                     /locus_tag="ACFOF2_000464"
     CDS             complement(12849..13157)
                     /locus_tag="ACFOF2_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106840.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(13141..13443)
                     /locus_tag="ACFOF2_000465"
     CDS             complement(13141..13443)
                     /locus_tag="ACFOF2_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC7106841.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(13517..15880)
                     /gene="pheT"
                     /locus_tag="ACFOF2_000466"
     CDS             complement(13517..15880)
                     /gene="pheT"
                     /locus_tag="ACFOF2_000466"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC7106842.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(15916..16434)
                     /locus_tag="ACFOF2_000467"
     CDS             complement(15916..16434)
                     /locus_tag="ACFOF2_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106843.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(16441..17505)
                     /locus_tag="ACFOF2_000468"
     CDS             complement(16441..17505)
                     /locus_tag="ACFOF2_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC7106844.1"
                     /translation="MIKSFELENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKDKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(17682..18674)
                     /gene="pheS"
                     /locus_tag="ACFOF2_000469"
     CDS             complement(17682..18674)
                     /gene="pheS"
                     /locus_tag="ACFOF2_000469"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC7106845.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(19020..19379)
                     /gene="rplT"
                     /locus_tag="ACFOF2_000470"
     CDS             complement(19020..19379)
                     /gene="rplT"
                     /locus_tag="ACFOF2_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC7106846.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(19392..19589)
                     /gene="rpmI"
                     /locus_tag="ACFOF2_000471"
     CDS             complement(19392..19589)
                     /gene="rpmI"
                     /locus_tag="ACFOF2_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC7106847.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(19736..20257)
                     /gene="infC"
                     /locus_tag="ACFOF2_000472"
     CDS             complement(19736..20257)
                     /gene="infC"
                     /locus_tag="ACFOF2_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC7106848.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(20275..22188)
                     /gene="thrS"
                     /locus_tag="ACFOF2_000473"
     CDS             complement(20275..22188)
                     /gene="thrS"
                     /locus_tag="ACFOF2_000473"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC7106849.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLGAEYVTENNDRSRPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(22242..22318)
                     /locus_tag="ACFOF2_000474"
     tRNA            complement(22242..22318)
                     /locus_tag="ACFOF2_000474"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22282..22284),aa:Val,seq:gac)
     gene            22497..23612
                     /gene="tgt"
                     /locus_tag="ACFOF2_000475"
     CDS             22497..23612
                     /gene="tgt"
                     /locus_tag="ACFOF2_000475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC7106850.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            23622..24704
                     /gene="hemH"
                     /locus_tag="ACFOF2_000476"
     CDS             23622..24704
                     /gene="hemH"
                     /locus_tag="ACFOF2_000476"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC7106851.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            24896..25297
                     /locus_tag="ACFOF2_000477"
     CDS             24896..25297
                     /locus_tag="ACFOF2_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC7106852.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(25360..25650)
                     /locus_tag="ACFOF2_000478"
     CDS             complement(25360..25650)
                     /locus_tag="ACFOF2_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106853.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
     gene            complement(25803..26123)
                     /locus_tag="ACFOF2_000479"
     CDS             complement(25803..26123)
                     /locus_tag="ACFOF2_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC7106854.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
     gene            26319..27045
                     /locus_tag="ACFOF2_000480"
                     /pseudo
     CDS             26319..27045
                     /locus_tag="ACFOF2_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            27114..28652
                     /gene="lnt"
                     /locus_tag="ACFOF2_000481"
     CDS             27114..28652
                     /gene="lnt"
                     /locus_tag="ACFOF2_000481"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC7106855.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFSLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28916..29779
                     /gene="rpoH"
                     /locus_tag="ACFOF2_000482"
     CDS             28916..29779
                     /gene="rpoH"
                     /locus_tag="ACFOF2_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC7106856.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29838..29984)
                     /locus_tag="ACFOF2_000483"
     CDS             complement(29838..29984)
                     /locus_tag="ACFOF2_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106857.1"
                     /translation="MLIHYINFANLSEKQLMRQCLVHVCFLISAIAMAWTDKIVYGTT
                     HKPH"
     gene            30263..31147
                     /locus_tag="ACFOF2_000484"
     CDS             30263..31147
                     /locus_tag="ACFOF2_000484"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC7106858.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIDEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(31231..31746)
                     /locus_tag="ACFOF2_000485"
     CDS             complement(31231..31746)
                     /locus_tag="ACFOF2_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC7106859.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHLLGISPKDAFGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(31822..32241)
                     /locus_tag="ACFOF2_000486"
     CDS             complement(31822..32241)
                     /locus_tag="ACFOF2_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106860.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(32362..33360)
                     /gene="holA"
                     /locus_tag="ACFOF2_000487"
     CDS             complement(32362..33360)
                     /gene="holA"
                     /locus_tag="ACFOF2_000487"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC7106861.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNGLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            complement(33362..33841)
                     /gene="lptE"
                     /locus_tag="ACFOF2_000488"
     CDS             complement(33362..33841)
                     /gene="lptE"
                     /locus_tag="ACFOF2_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC7106862.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(33893..34672)
                     /gene="pgeF"
                     /locus_tag="ACFOF2_000489"
     CDS             complement(33893..34672)
                     /gene="pgeF"
                     /locus_tag="ACFOF2_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC7106863.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALGGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
BASE COUNT         8698 a         9418 c         8132 g         8651 t
ORIGIN      
        1 tgagaaatga gaaatgagaa atgagaaatg agaaatgaga aatgagaaat gagaaatgag
       61 aaatgagaaa tgagaaatga gaaatgagaa atgagaaatg agaaatgaga aatgagaaat
      121 gagaaatgag aaatgagaaa tgagaaatga gaaatgcctc cgatataatc ggaggcattt
      181 tgaattggca cgcccacggg gaatcgaacc ccggttaccg ccgtgaaagg gcgatgtcct
      241 aaccgctaga cgatgggcgc gtattgtatt ttcttactgg cgcacccgga gcgattcgaa
      301 cgcccgaccc tctggttcgt agccagatac tctatccaac tgagctacgg gtgcagcaca
      361 atcacttgtt caagtgatta agaaagcgta ataatacgga tttatctgag ttctgtctat
      421 tgttttagac ttttattttt agttattttt taactgattg attttaaaat aaactatttt
      481 tcgacgcaag ttgcagcaat tctgccgcat gttgccgcgt cgtatcggta atgacttctc
      541 cgcccaacat acgggcaacc tcttcgaccc gttggatttc atccaatata ctgatttcgc
      601 tgacggtttg ctctccctcg ctgtgcttgc gcactcgcca gtggttttcg ccgcaggatg
      661 cgacttgcgg aagatgggta acggcaagca cctgatgttt tctgcccaac gcacgtaatg
      721 ccttgccgac catttcagcc actccccctc caataccggt atcgacctca tcaaaaatca
      781 gggtgggaac ttgggtatat tggctggcaa caacctgtaa ggcaaggctg atacgcgcca
      841 attcgccgcc ggaggcaact ttattcagcg gacggggcgg attgcctttg ttggcggcaa
      901 cttgaaattg aacctgctcc aaaccgtgtg ccgtcggcga cgagggcaac aggacgatgt
      961 cgaaacgtgc acctttcatg gcaaggtgtt gcatatgctc ggtcgtttcg ccgctcaaac
     1021 gctctgccgc ctgatggcgc atggcagaaa ggatgtgggc agcttcctga tattcggcaa
     1081 aattgtgggc aacattatgc tcgagcgcgt ccaaatcggc ggcagcttgc aggctttgca
     1141 ggcgttcttc gatttctgcc aacttggcag gcaactcttc aggctcgatc cggtatttcc
     1201 gcgccatccc catcagctcg cccatacgct gctcttgtgc ggcaagttcg ttgggattga
     1261 tgtcgctgcg acctgccaca tcgcgcatat tggcactgat ttcgcccaat tcggcttcga
     1321 tgcttgccaa catattcagg ctctcggcaa agcgcggctc gatgttttgc agattggcca
     1381 atagtttttg acattgatag atatggcgtt ggatgccgtt gtcgccgtca atcttgcttc
     1441 cgacttcttc ggcagcctgc aacagctcgg cagaatgggc aaggctgtcg tggctttggc
     1501 tgagggcttc ccattcgcct tgtttaatgt ccaactgatt caattcgtta aactgccatt
     1561 ccagacgctc ttgttcgagt gcaatgtttt ctgcctgttc ctgcgcttcc tgcaatgctt
     1621 tttttgagtt tgcccagttt tgataaagct gcctgacggt ttccgcctgc gccctgctgc
     1681 ccgcaaatgc gtccaacaat tcgcgctggg cggcttcctg attaagcgaa tgatgggcgt
     1741 tttgcccatg gatgtcgata agctggctgc cgacggcttt gagttgcgcc aaggtagcgg
     1801 cctgattgtt gataaagctg cggcttttgc ctttggcatc gataatgcgg cggatactga
     1861 gttcttcttc tccgtcgttt aaaagcccct gttcatacaa ttctgctttt aaaacaggta
     1921 aatgggaaat atcaaacaac gccgacaact gcgcttcttt tgcgccgctg cggacttggc
     1981 tgtaatcggc tttatcgccc aacagcagac caatcgcatc caaagtaatg gacttgcccg
     2041 cgccagtttc tccggtcaat acggtaaagc cgctttgaaa atccagattc agattttcga
     2101 caatgacaaa atcacgcaaa gaaagtgtta gaagcatcgt gaggattcct tagcaattct
     2161 accgacaaaa gcaatatcaa ctttagatat acgaattata actaagattt tttaaattaa
     2221 aaataagttt ttccttaatt ttgcttagtg cttgttttta tctgcttatt tgttgaaatg
     2281 ccgtctgaag caatgtgcgt ttcagacggc atttggaatt tcagttgggc agggtatcgg
     2341 acggtacggc ttccggctct tcttcatccg taggcggcat ttccattaaa tcgggtaaaa
     2401 ccagttcgcc cagttcggtc agcggcggaa gttcttccag accgtccaag cgcaaatcgc
     2461 tgagaaaagt tgccgttgtc gcccacaatg cgggttttcc caatgtgtcc cgatgtccga
     2521 tgacttcaat ccacccccga tcctgcaagg tctgcatcac gttctgcgac accgccacac
     2581 cgcgtatgcc ttcgatgtcg ccgcgcgtta cgggctgctg gtaggcgata atcgccagtg
     2641 tttccatcac ggcgcgggag tagcgcggcg cacgctgttc ttgcaggctg cccagccgct
     2701 cgaatgccgt ctgaacaatc tgaaaacgcc agccctcttg cgtatgcacc agttgcaacg
     2761 ccctatcctg ccaacgcgtt ttcaactgcg ccaacacatc aatcagtttg tcctgcgaca
     2821 gtggcggcac acacaattcg cgcatagatt tttcggtcag cggttcggtt tgggtcagca
     2881 gtgcggcttc aatcagcgcg tcgggagaaa ttttgtcggt catacgggta tccgtgctga
     2941 aacggcatgg gtttggatat gccgtctgaa atcggttgga gtagagagaa gctgcctgaa
     3001 aaatattttt cagacggcat tctttatgct tccgaaggct tcttcccttt acgctctgct
     3061 tttctggctt cgcgtatggc tttgagttcg gcttctttct gacgggcttt tttgagccaa
     3121 gtttcccatt ggttcggcgt ttcgagggtg attctgccga ttttgccttc acggaagtcg
     3181 gtgaggatgt tttcggcggc tttttggtag ttgatccgtc cgccgctgag gactgcgccg
     3241 cgttttttgg ctatccattc gagccaaacg ttttcgtccc agtggctgct ggggtctttg
     3301 tcggcttggt agcgttcttg caacataggg aggtagtggc ggcggaggta gtctaaaagt
     3361 tcgagggcga cttcttcttc gtccaatgcg ttgcgtccga ctgcgccgcc ggcggcgagg
     3421 ttgtagccgc tttcttcgac aatgattttc ggccacagca ttccgggggt gtcgtacagc
     3481 cagaagtcgt cggcaaggaa aaggcgttgt tcggctttgg tgatgccggg ttcgttgccg
     3541 gttttggcgg attttttgcc tatcatgccg ttgatgaggg tggatttgcc gacgttggga
     3601 atgccgcaaa tcaagacgcg caagggtttg tctatgcctt gccgatgggg aatcatcgcg
     3661 cggcaggctt gggtgatttt gccgtgtgcg ccggtttcgg aggaatcgag ggcaatggcg
     3721 cacgtgtcgg ggcggctgtt gtagtggtca agccagacct ttgtgcgttc ggggtcggca
     3781 agatcttgtt tgtttaggat tttaagtttg ggtttgcctt tggaaagctg ggcaagcagg
     3841 gggttttcgc tggaggcggg cattcgcgca tccagcattt caatcaccat gtctacgctt
     3901 ttggcgcgct cggcgatggc ttttttcgct ttgttcatat ggccgggaaa ccattggatt
     3961 gccatgtctt tcctttcttc tcaatatttg aaatgccgtc tgaaacagaa aatcgggttt
     4021 cagacggcat catcgttcaa cgggattagc ggctcggcag gttttgcgcc tgtctgtgcc
     4081 gtatcagcaa ttcataacgc cctttcaacc ttcgggcgag tttttcgaca atatagaccg
     4141 aacggtgctg tcctcccgtg caaccgatgg cgacggtaac gtagctcctg ctttcatcct
     4201 ccaaacgcgg taaccaatgc gtaacaaacc tttcgatgtc gtcaaccatt tcctgcacaa
     4261 gcggctgtcc gtccaaataa tcccaaacgg gcttgtccat accggtgtaa ggcctcaact
     4321 cgggatcgta atacgggttg ggcagactgc gcatatcgaa cataaaatcc gcgttgttcg
     4381 gcacaccgta tttgaacccg aaggactcca aaatcaccag cagcccggta cgttcgacct
     4441 tcagccactg ccggactgca tggcggagct gttgggcatt catcttggaa gtgtcgatac
     4501 aataggcgat ttctttaagc gggaacagcc attcccgttc tttctttaag ctttccaaca
     4561 gggtcatatc ctgattgctc agaggatgtc ctcgcctggt ttcggaaaac cggcggacca
     4621 acacgctttc ttccgcctcg acaaacaaaa cttcaaccct gtgccccagt ccgcgcagag
     4681 aggcaatctg ttcccgcgcc tgtccgatgt caatgccgga acgtacatcg acgctgaccg
     4741 ccaattcggt ttcgtccgca cgctcgatat gatacgacac cagcgcgggc aacatttcca
     4801 agggcaggtt gtccacgcag aaataacccg aatcttccat ttggcgcagt gcgacggatt
     4861 tgcccgaacc ggacaacccg ctaatcagga cgattttcat gattctgttc gttttcttta
     4921 agttgcgtct gatggcgttc caaaaattcg cgcgtactgt ccttaccgcg caactgcaaa
     4981 atgtaattgc gtaccgccgc ctcaaccaaa acggcgaggt tgcgtccgac ggcgacgggc
     5041 agcgtaaccg aacggacgtt gacgttgagg atggattcgg tttcggtgcg gatactcaac
     5101 cggtcgagct gcttcatata ctcgtcgtct gcctcgacca aattgataat gagttgcagg
     5161 atttttttgg ggcggatgga agtttcgccg aaaatatggc ggatattgag tatccccaag
     5221 ccgcgcactt ccaaaaaatc acgcagcata ggcgaacaac gcccttccaa tgtttccgga
     5281 ccgatgcgga acagctcgac cgcatcgtcg gcaatcaggc tgtggccgcg cgaaatcagt
     5341 tccaacgcca attcgctctt gcccaagccg gaatgtccgg taatcagcac gccgatttca
     5401 aacacatcga gaaatacgcc gtgtttgacg gacgatgccg ccagggtgcg ttgcaggtaa
     5461 atccgcaaca cgtccatcag ataggggctt tcgagtttgg aagtcagcag tggaatatcg
     5521 tttttatggc aatagtcgcg cagccccggg gaaaccggca agccgtttgc cacaataacc
     5581 aaagacatag aaatgtcgaa caggtcgccg aactgataac ccgtttcccc cgattcgaga
     5641 cggttcagat attccgattc cgccaaaccg accacttgga tttggttggg gtggatgaaa
     5701 ttcaggtgtc cgaccaaagc gaggacgggc ttgtccgcct ctacgccgat acggttgtcc
     5761 gcacccgaat tgccggcggc ccaggcaagt tgtaacttgt attggttgtc gtcaaacagt
     5821 cggcggacgg aaatactggg catattattc ttcagtcaaa atggcgcggg cttcttccgc
     5881 agaagaaacc gtcatcagcg attcgcggat gcttttttgg gaaaacttgc cggccagttt
     5941 ggataagact tccaaatgtt cgccagttgc gttttccggc accagcaaga taaaaatcag
     6001 ggaaaccggc ttgccgtccg gtgcgtcaaa tccgacgggt tcgcgcgtgc ggatgaacgc
     6061 gcccgtcgcc tgcttcacgc cggcatgacg gccgtgcggg atggcaacgc cctgccccaa
     6121 accggtcgaa ccgagttttt cacgggcaaa aagacattcg aaaacatcag catgggacaa
     6181 tgaggattcg cgttccaaaa gcaggcccgc ttcctcaaac agcctttttt tactgcccac
     6241 ctccatatcc aaaacaatat gggacaaagg caaaatttcg ccgataaggc tcataagctt
     6301 ctcttttcag acatcgcaaa acagaaagat tgtaccgact gctagggcaa atctcaatcc
     6361 cgcatacggt acgggctgac ataacacagc gttttaaaaa catattttaa cgcttttcgg
     6421 cacagataga aatgccgtcc aaagcagttt acggctcttc agacggcatt gccctgcctt
     6481 atttcgcgaa aaacgtatgg gcaaaaatgg cggtaaacca cacccaacca attctgttgt
     6541 ttgccaaaaa cgtttcaaaa cagatttgcc ggacgcggct tttgatggcg gcatattggc
     6601 ggtattgcag cagcaggacg atggggattg ccgtccaata tgcccatgcc gcaccgataa
     6661 ccgcacccaa tactgccatc agcagggtaa agccgccatg acacagcata acggcggcga
     6721 tgtcgtaacg cccgaacgtg acggcggagg ttttgatgcc gattttcaaa tcgtcttctt
     6781 tgtccgccat tgcataaacc gtgtcatacg ccagagtcca taacacattg gcggcaaaga
     6841 gtatccacgc ttgaggcggc acgtttccgg caacggcggc aaacgccatc gggataccga
     6901 aggaaaaggc aagcccgaga tagagttggg gaatcggaaa aaagcgtttg gtaaacgggt
     6961 aagtcagcgc aagaaacagc gcgggcaggc tcatcagcca tgtcagatga ttcagcggaa
     7021 tcaggcacaa tgcggcaagc aggcacaaaa atgccgtcag cagcagcgct tctttttttc
     7081 cgacccgacc ctgtgcgaac ggacggtttt tggtgcgctc gacagcaccg tcaaaatcgc
     7141 ggtcggcaaa atcgttgatg acgcaaccgg cactccgcat taaaaacgtg ccgattgtaa
     7201 acgccgccaa taccgccaaa tcgggaatgc cgtctgaagc cagccacaat gcccagtagg
     7261 tcggccacag caaaagcagc gtcccaatgg gcttgtccgc ccgcatcagg cgcaggtaca
     7321 catccaaacg atcggacagt cgtaaaaata aaggggattt aggattcata ttgccgcaca
     7381 gcttgaaaaa acggtatttt atccgataaa gcgtttcagt tcgggcagaa aatactcggt
     7441 cagcagcatt tcctcgccgt gacgggaaaa ccgagaacgc cgcgcggcaa agtaccgtcc
     7501 gcatccttcg ccggaaacgg caaactcaaa cgccgaacgc gccccttcca aatcggcttg
     7561 aaacagacgc tcgcccaaag gacgcgtgcc gcagtccaaa atgttttgcc aaaacgccga
     7621 accgatacgg cactcgctcc ttgccgcgac aacagggata ccgtccagct tcaacaaaac
     7681 ttcgcgcacc agcctccctc cgcattccgt ctccaattcg cccagtttca gcagttccac
     7741 cgaaaatgta tgcggcaagg cgcgcaatgc ggcggtcagc gaccgggctt tcaataaccg
     7801 gctcatcggc aggccgatgc cgtctgaaac aggggcgggc aggtcgggca gccattcctc
     7861 aaagccgtaa cccattatta ccattttgtt gaatatcctt tattattaag catacatctt
     7921 gcattatccc caatacaatt tgtcgatttg actgcattat cattgtattt attatcataa
     7981 tattttttta taatatattg ataattaccc ttttcatcag gtaattgata taatttatat
     8041 atttcaggac aagcaacgga aaggcaactt ctgaaatcat caattcgttg ttcgttagtc
     8101 gtattatttt taacaaaata ttcaatatcg ttgtttgctc ttatcgtcat atcatcaaaa
     8161 ggataattac aagacgaagt tgcaaaaaag caaggattac aagaagttgt cattaccatt
     8221 ataaaaacaa taatgacaac tttaaaacaa cgcgtaatca taaatcaatg cccgcttaat
     8281 ttcataatca cttggataaa gtcgctatca actatatcgg caatagcaaa tttaatggtt
     8341 ttcatttaac aatttctcca tttctttttt ataatctatt ttctgaaatt ctgcggtttt
     8401 ttgataataa tcataaccac ccaaagaact caaacaaata atgtgcatat ttctaactgc
     8461 attataagcc aattccttat catttcttgg aatacctttt tctttaagca tgaaaatgta
     8521 attatcaaaa tcttgcggaa tatttaattc ccgtatattg gaaaaataca tattagcaca
     8581 atatttaata tcactcctaa cttgatttat gcttttatta ggattttttc taataacatt
     8641 ttcaatatca gaacttacag gacaatgaac gctttgtggg gcaacaaagc atttttcatt
     8701 atcaatagtt gtgtaataac agccctgcat tatcaacaat acaagggctg ttgataataa
     8761 gcggaacatt accattttgt tgaatatcct ttattatttt tcatgcattc tccaatcttt
     8821 ccgtagcatg tcatgacatc atctgcattc ttatagccct ttttataatc atctttatat
     8881 tttctacgaa taatctccct ataatctcca ttttcatctg gtagttgata tttttgataa
     8941 acttctggac atgcataaga aatacaatag cgtaaatccg caattcttgt ttctttgctc
     9001 gtattattct taacataata ttcatcgtcg ctatttatag gatagttttt tctaaaagaa
     9061 taatcacaag aagaagttgc aaaaaagcaa ggattacaag aagttgtcat taccattata
     9121 aaaacaataa tgacaacttt aatatagcat ttcataacaa ccaccaatat atagataatc
     9181 aatgaccagt aattttcata atcgcttgga taaaatcctg atcgactata tcagcaatag
     9241 caaatggagc ggctactggt atcgctgcaa cagttacttt acttgaaata gtttcatctt
     9301 ttttatttgt tgttcttcta tttggcgttg tatttccata ttcatcatat aggtgttcca
     9361 tatttttccc aatctttata ccgcgtctgt ttttgccaac tccatccatt ccgcttcgga
     9421 aaaatcggct ttcagacggc atttcaagta gctcaggctg tcttgggcga tgtgtgcgtc
     9481 gcggctgtcg tggatgtggt tgaacacgag gctgtacaag cgaatgccgt attgtttgag
     9541 cgctgcgaaa ctgagtaaag tgtggttgat actgccaagc cgtccgctgg taacgaggat
     9601 gacgggatag ccttgctgac ggatataatc aatggttaac aggttttccg tcagcggaac
     9661 catcaatccg cccgcgcctt cgaccaaaac gacttcgtac tgcgccgcca attcttgtgt
     9721 ggcggtgcgg attttgtcca agtccaaagc cctgccgtca agacgggcag cgagatgagg
     9781 cgaagctgga taactgaaga tttcaggcat ggtcagccct tgtttgtcgg cttcctgcat
     9841 gggtttaccc atgattttac gatggacggc gatgtcgtcg gcaatgtttt ggcaaccggt
     9901 ttgcacgggc ttttgcgtaa tcacgctttt gccctgctgc aacaattgtt ttgccaacac
     9961 gccggtggcg acggttttgc cgatgtccgt gtctatgccg ctgacgaagt aaacgccttt
    10021 catttgctgt gttccttcaa gatttgcacg gttttgtcgg caagtttggt caaaacgccg
    10081 tctgaaatga tgtaaggcgg catcagatac accaacctgc cgaacgggcg cacccaaatg
    10141 ccctgcgcca cgcaatccgc ttgaaaacgc gccatatcca cgcctttttc caactcgatc
    10201 acgccgatgg cgcccaaaac gcgcacgtct ttcacaccac gaatgtccca cgcggttttc
    10261 agacggcatt ttaagatgct ttcgatgcgg cggatatttg cctgccaatc ttgggacaaa
    10321 agcagtttga ccgaagcgca ggcaacggca cacgccagcg ggttcgccat aaatgtcggg
    10381 ccgtgcatga acacgcccgc ttctccgcgc gaaatcgttt cggtaacttt ttgcgaagtg
    10441 attgctgccg ccagcgtcat atagccgccg ctcaaaccct tgccgatgca cataatatcc
    10501 ggcacgacct ccgcgtgttc gcaggcaaac atcttgcccg tgcgcccgaa tccggtggcg
    10561 atttcatcaa aaatcaaaac gatatcaaac tcgtcgcaca aatcgcgcaa tccgcgaaga
    10621 tactgcggat gataaaaata cataccgccc gcgccctgca cgaccggttc caaaataaag
    10681 gcggcaatgt ccgtatgatg cgcttcaaac aaggcgcgga caggctgcaa atccgcctcg
    10741 tcccattcat cgtcgaaacg gcttgtcagg ttatcgacaa aataacgctg cggcaacgcg
    10801 ctgccgaaaa tatggtgcat ccccgtttcc ggatcgcaga cggacatcgc gttccaagta
    10861 tcgccgtgat acccgcgccg caccgtcgcc atattttgtt ttgcacgcaa accccgcgcc
    10921 tgctggtatt gcaccgccat cttcaacgcg acttccaccg aaaccgaacc cgaatccgca
    10981 taaaaaatac gatccagccc ctgcggcaaa atcccgacca acaatttgcc cagctccacc
    11041 gccggctcat gcgtcaaacc gccgaacatc acatgcgaca tctgtttcat ctgcgtctca
    11101 accgcctgat tcaaaacggg atgattgtaa ccgtgtatcg cacaccacca agacgacatc
    11161 ccgtcaatca gccgcgtgcc gtccgccaat tcgataaaca ccccttctgc acgtttgaca
    11221 ggataaacgg gaagcggatc ggtcatggaa gtatagggat ggagcagatg ggtacggtcg
    11281 aaatcaagca atgatgatgt gtgttgatgt tcagacggca taagtttctc tcttttctta
    11341 ttgctgcatt caaacgcaaa acgcgtattc tactccgaca gaccgcttcc cacacctttc
    11401 catccgtttc gggcgtaaaa ccacgcaaca aatcgtccgc agtataagcg cacaccgttt
    11461 cgcattcccc aagcccgatt ggaatcagac gacccaacgc ccaatattgt ttcacacagc
    11521 ctccgcccaa cgcttcaatc agtcatagac aaaacggcaa tccatacgat acagatcata
    11581 gcaacagcca tcgcaacagc gttagcaaaa tcaggggact ccgacatagg cgcatagcat
    11641 ctaccgatgc acagctcctc attcggctct atgaatacca tacccatcac aaaatccacc
    11701 gccaaaacca ggcacggctt cttatactta tgatagattt ccaccatcct gccccatata
    11761 tcccaaacat tcataccgca tatcccgcag gcaacaaatt ccgattgaag gttacagccc
    11821 tattttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
    11881 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
    11941 ggcgaggtaa cgccgtactg gtttttgtta atccactata ttttcaaacc ggaaaaatgc
    12001 cgtctgaatc ttcagacggc attccattca tatttattcg tcttttttgt gttcgatggc
    12061 gtttcgggaa cgattgccgc cgtaaggctc ttcaaccgta tattccccct cgataatatc
    12121 gtcatcgcgg gaaaagccct cttttctgcc cgattggttc atgttgaaaa aattttccgc
    12181 acctcctgcc tgcaacactg cacctccctt aaacggcagc agcagcaaca ccgccaacac
    12241 cgaggatacg aatcccggac tcatcagaca caccgccgcc accgtataac ggataggcca
    12301 caacatctga taaacggata ccctcccgcc gcttctcatt gccgcgcccg ccaataaaag
    12361 accggacagc cccgtatgcc tgagcatcag cacaccggcg gcaaaacctg ccgccatcaa
    12421 aaacaacgtc cagccgccgc ccaaccaatc ggcaacccac acaatcgaca taatctccaa
    12481 aaacagcagc accaaaaaac cgataccgaa aaatctcatt gaccgtcatc cttatattta
    12541 agtaaacagc aaaccgcccg aacaggactc caagcgacct gcctgtaaat gattacaaaa
    12601 ccatgtgctt caagccgaaa caatgtgaaa tcacgcaata tagtggatta acaaaaacca
    12661 gtacggcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa
    12721 gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
    12781 ttaatccact ataattattt tttagcgtgt aaaacaaaac cggctgcata cctgcaaccg
    12841 gcctcaaatc agcacaattc cttatccaaa tccgccaaca ggtctttcag accgtctccg
    12901 atttcctgaa gcgtaaccgg acggttgccg tccgaacccg attccgcacc ttctgcatac
    12961 ggtgcaaacg tgctctgcaa tttcaacagt ttcaccacat ccaaacgggt gtagcgttcg
    13021 ccgccgtaac cgacaaccac accgtgatta tgctgccatt gcgcaaaacc ataggggctg
    13081 atttgcaaca gtccgcacaa ctcgtccagc gtgaaatagc gttttgcggg aaccttcaaa
    13141 tcaacgttgt ttgtcatagt agtgttccac catgcttttg agtttctggc tggcatggaa
    13201 agttaccacg cggcgggcgg taatcggcac ttcctcgccg gttttaggat tacgacccgg
    13261 gcgttgcggc ttgtcgcgca actggaaatt tccgaaaccg gaaattttga tttcttcgcc
    13321 gcttgccaaa gtgctgcgga tttcttcaaa aaagagttcg acgatttctt tggcatcgtt
    13381 tttggtgacg ttgctgactt tgtctaccaa aatatcggcc agttctgctt tagtgagagt
    13441 catatgatta ccttctgttg taacagttgt ggattattac caatttttat ttaaaattca
    13501 agcgaatatt tattttttaa ctgcgaagcc gcgcccctgc cgccgttgcc gcgccaatca
    13561 gttttccgat aagcggctcg accgcctcat ccgtcagcgt gttttccata tcctgcaaaa
    13621 tcactttgac cgccacgctc ttcatccctt cgggcagtcc cgtgccgcga tacacgtcaa
    13681 acacgctgat ttcctgtacc aacttgtttg ccgcgccttt caagacaagc agcaaatcat
    13741 catggctcat agcttccggc atcacaaacg ccaaatcgcg gcgcaccggc tggaatttcg
    13801 atacggcccg atagcgcgtt ttcccgcatt ccaacacggc cgccatatcg atttcaaata
    13861 ccagcggcgc ttgcggcagg tcgtattttt gcagccattt cggatgcagt tcgccgacaa
    13921 agccgatgac gttgccgtct gaaacgatat tggcggcgcg tccgggatgc agggcaggat
    13981 gtccggtttt aacgaactcg accgctttgt ttttcaacag attttccaca tctgccttaa
    14041 tgtcgtaaaa atccgcattg cgcgttttcc cgccccattg ttcgggcatt gccgcgccgt
    14101 accacaagcc gccgatgcgt tcgttttgga caaaccggcc gtctgaacct ttgccgaaca
    14161 cgcgggcgat ttcaaacacg cgcacgcggt tttgtttgcg gttcaggttg ttttgcaaaa
    14221 tttccaccaa gccgccgatg agtgtggaac gcatcacggc atattgcgcc gccagcgggt
    14281 tttgcaggcg gatggggtcg gcgttggcgg caaaatcttg ttcccactgc tcgtcaacga
    14341 aggcatagct gaccacttcg cggtagccgc gcgccgccat ttcgttgtaa acggcaaaac
    14401 gcgggcggcg tgtttcgggc agttccagca ttttcagacg gcctgacgtg taatcgtcgg
    14461 ggatgttttc atagccgtaa acgcgtccga tttcttcaat caaatcagcc tcaatttcga
    14521 tgtcaaaacg gaagctcggc gcggtaacgc ggaagccttc cgccgttttc tcgggctgca
    14581 ggcccaagtg ttgcaaaatg gtttccacct gttcggcagg aatgtccacg cccaacacgg
    14641 ttttcagacg gcccaaacgc aatccgacct gcttcgcttc gggcaattcg ccttgcgctt
    14701 ccaccatttc gcctgccgca ccgccgcaaa tctgcaacac caattcggta gcacgctcaa
    14761 tggcatccgc ctgcaaacgg taatccacgc cgcgctcgaa gcggaacgac gaatccgaac
    14821 cgaaaccgta ttggcgcgat ttgccggcga tgatttcggg cgcaaaccaa gccgcttcca
    14881 gcacgatatt ttgcgtgccg tctgaaaccg cgctcgccgc gccgcccatt aagcccgcca
    14941 aactcaacac gcctttttcg tccgccacga ccagcgtgtt ttcagacagg gaaacggttt
    15001 tctcgttcag gcattccagc gtttcccctt cgcgcgcgcg gcggatgtgc aggctgccgg
    15061 aaagtttgtc ggcatcaaaa acgtgcatcg gctgaccgat ttccagcatc acataattgc
    15121 cgatgtccac cagcgcggaa atactgcgga tgccgctgcg ctccaaacgt tgtttcatcc
    15181 aatccggcgt agtagcgcgc gcgttcacgt tttcaatcac acggctgata aagcgaccgc
    15241 aatcggcagg cgcgtcaatc tgcacgggct gttttcgact gcccgtgatc ggcgcggtat
    15301 ggatttcggg ctgcctgaac gcgcaccccg tcaatgcgga cacttcgcgc gcaatgcctt
    15361 tgatgctcaa gcagtcggcg cggttaggcg taattttcaa cgtaaacagc gtatcgtcca
    15421 aatccaagta ttcgcggata ttggtaccga cgggcgcatc ttcaggcaga atgtgcaggc
    15481 cgttcacacc gtcgtcgggc agaccgagtt cgtcggtgga acacaacatc ccgtccgaca
    15541 ccacgccgcg cattttggtc ggcttgattt tgaaattgcc cggcaacact gcgcccggca
    15601 acgaacacgg cactttgatg cccgctttca cattcggcgc accgcacaca atctgcacca
    15661 acccgcccgt acccgcatca actcgggtaa cgttcaaacg gtctgcatcc ggatgttttt
    15721 caacggattt cacttcggca atcaccacgc ccgcaaacgc aggcgcggca gtttcggcct
    15781 cttccacttc caagccggac atcgttaaca gatgttccag cttatcggag gaaagttcgg
    15841 tatcggcttg ggttttcagc catgaatagg agaattgcat gttatgttct caatcaatta
    15901 attaacttta gattttcaag agctagctaa acctttttga atggctaagt ccctattaat
    15961 tacttctaca gaaatgttcc ataaagaaat caaccgcaaa gtatctttct tattaaattc
    16021 ttgctttatt aattcattca attgcccaag ataattaagc ggcacatccg ggataaccag
    16081 taataatttg atttctatgc taccccattg caaatttgaa aaagcactag gcataattgt
    16141 ttctttgata tcttctctgc ctaatattcc caaaatttca acatttaatg cattacacca
    16201 tttttgataa atctctgaaa tatattcatc aaaacgctca ggagatcttt cttgatttgg
    16261 aatactttgt tttgcctcaa caaataacaa attatcttct tgcagataga taaactctac
    16321 tgtcttaata ccttcttgcc ttctcttccg gatatatggt tctatataaa acaggttatt
    16381 ttttactaga ttaaaagtta tacctgattc agtgagattt attatttcac tcataaaaac
    16441 ctaaccaatc catgctgttt ccggagataa aacacctatt tcttctctga acaatcgaat
    16501 agactcacca ataatttcat tatccggcat gctattttgt aaattttcct gcacccaagt
    16561 agtctgttcc ttgtctttgc caggcataaa aacaggaata ctttcactat gctttctagc
    16621 taataaacac agcttcttga taacaaaata tgaatgcgta ctcataaaaa tctgtattcc
    16681 caatttggat ttagataagg aaaacagaat ttctagaaat tgattaattg ctttcggatg
    16741 tagattagct tctggctcat caataaataa gattgatcca ggactcaaaa accgatttcc
    16801 taatagagtg tctaatatcc caatcttttt taccccctct gctgtagaat tgatagagaa
    16861 agtttgtcta tctttttcat atacccatgc cttcttttta tcattgtagc taacataacc
    16921 gccaaacaat gcttctagat cctttctagc ttccgctgcc tcagggtaat tcctcccttt
    16981 tgttggttta atatccaatg cttttaccaa atcaatgtaa gtagcatcaa aaccaaatct
    17041 cttttcttcc tctgattttt taattacatc aaatagtgat agaacttctt tgggtggtaa
    17101 aaaaatagag ttagcgttta ttctgggaga aagttcgtat agctccggtt taattagctt
    17161 tttggtagtt ctaccaaaag aaaacaaaac tgaagaacta tcttctaaag tcatacttaa
    17221 cttaagaggc cgttcgtttg gattttcttt tcctcttgta actaaatcac caatttcctc
    17281 tacttggaac gtccaatata gcttatctcg caattcctca gaaaaatcac gaatattctt
    17341 tcctttatga gcttcctcat gactttttaa aacactatat aatgccttta aaataaaagt
    17401 tttccctttg ctattttctg caataattaa atttattttc cctaaattat cagccttcaa
    17461 taagctaatc ggcccgaaat tttcaagctc aaatgattta atcatataat ccccctaata
    17521 tatatcaaca atatttgtac aagccacatt gtaaaaagac agcaaccaca gcaccatagt
    17581 aaacgcatag caggcggata atggagcgac atagcgtttc atgatttagc tttctgaggt
    17641 aggcacaggc tgcttttttc atttttaggc agcctacaca atcattttac aaactgcttc
    17701 aaaaaattca aatcgttatc aaaaaacaag cgcaaatcgt tcacgttgta acgcagcatc
    17761 gcgaaacggt cgagaccgat accgaaagcg aaaccggtgt atttttcaga atcgatattc
    17821 acatttttca acacgttggg atgcaccatg ccgcagccgc cgacttccag ccatttgccg
    17881 ttttctccca taatgtcgat ttcggcagac ggttcggtga acgggaagaa agacgggcgg
    17941 aaacgtactt gcaaatcatc gcgttcaaag aagcgacgga taaaatccgt gaacactgct
    18001 tttaagtcgg caaaagttac gccctcttct acccacaaac cttccgcctg atggaacata
    18061 ggcgagtgcg tggcatcgct gtccacacgg taaacgcggc cgggggcgat aatgcggatg
    18121 ggcggctctt ttttatcgag catatagcgg atttgaatcg gggaagtgtg cgtacgcaaa
    18181 acatcaccgt tttcaacgta aaacgtatcc tgcatcgcac gggcaggatg gtttgcaggg
    18241 atgttcaggg cttggaaatt gtgaaaatcg tcttcgattt caggcccgtc cgccacttcg
    18301 aaacccattc cgtgaaagag ttcgaccaca cgttgcaagg tcagggttac gggatgcagg
    18361 ctgccgcctt cctgagcgcg tccgggcagg gtaatgtcga gggcttcggc ggcaagtcgg
    18421 gcttgcagct tggcttcgtt gagggcatcg cgtttggcat taaaagccgt ctgaaaccgg
    18481 tttttgcatt cattgatatg cgcacctatg gttttgcgct cttcaggcga catttgcccc
    18541 aaagttttca gaagtccggt caactcgccg gttttgccaa gataacgggc tttgatttgt
    18601 tctagagcgt tgaagtcttg cgcagcttct actgcggcaa tgccttctgc aacgatgcgg
    18661 tttacatttt ccataatatc aagtctgtca attaatgata tgccacctga aacaacgagg
    18721 ccgcctgtat agcctgacat atcagactct tcagacgacc ttattttctt cacggtcaat
    18781 ccattggata aattttctgt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc
    18841 ttgccgtact atctgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc
    18901 actatatttt aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac
    18961 atagaatttc tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat
    19021 taagcagcca aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac
    19081 acggccaaat cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc
    19141 ataaatttgc tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac
    19201 aattgacgga attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc
    19261 attaccgcct gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct
    19321 aacgcgaaga ttttttggtg acgggcacgg gcggtaacac cgcgttttac gcgtggcata
    19381 ttctaaactc cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt
    19441 accatagagg taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg
    19501 cgtttgaacg catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc
    19561 gcgctagact tggttttcat tttaggcatg ggaaaactcc attcgttatc agataaggca
    19621 ttagggggtt ttaaaacatc ggtttcaaac acttgaaccc acaataccac ggttttttgc
    19681 cgcaattgaa aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt
    19741 ttttcttcgg cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga
    19801 tttgcgccac ttcagccaaa tcttctttca cacgttccaa aagttgcgcg ccgagttgct
    19861 ggtgagccat ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa
    19921 ggaagcggtt gatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga
    19981 atttgatttc cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg
    20041 cctgctggta tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg
    20101 gggaaatctc taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg
    20161 aaacaacacc aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt
    20221 cgccgttgat tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg
    20281 attgacgagg gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa
    20341 agaacccaaa tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc
    20401 gccgacaacg atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat
    20461 tttttcgtta cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac
    20521 ttcccgacaa taatctgcct gattttcggt aatattcata atcaccattt gaaccggagc
    20581 caaccataac gggaaagagc ctgcatggtt ctcaatcaga atgccgataa atcgctccaa
    20641 agaacctaaa atggcgcgat gcaacataac aggtcgcgag cggtcgttgt tttcggttac
    20701 atattctgca cccaagcgtt ccggcaagac gaaatccagt tgtaatgtac cgcattgcca
    20761 agaacgaccc aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc
    20821 gcccggcaat tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc
    20881 tgccttatcc cacacgtcat ctgaacctgc acgtttttca gggcgaagag aaagcttgac
    20941 ggatacatca tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg
    21001 ggcctcgctg acgatttggt cttcggtaca gaaaatatgc gcatcatcct gcacaaatcc
    21061 acggacccgc atcagaccat gcaatgcacc gctcggctcg ttgcggtggc aggaaccgaa
    21121 ttccgccaaa cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac
    21181 atgacccgga cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa
    21241 catattatct ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccatgat
    21301 ttgaggcgtt ttgacctctt tataaccggc ggcgttcagt tctttacgca tatgctgttc
    21361 aatcacttgc cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc
    21421 ttgcagatgg aacagatcca attgcttgcc aagcttgcgg tggtcgcgct tttcggcttc
    21481 ttcgatacgt tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtatat
    21541 gcgttgcagc atttcattat tgctgtcgcc gcgccagtat gcgcccgcca gcttggtcag
    21601 tttgaagttt ttcaggaaac gggtattcgg aacgtgcgga ccgcggcaca tatcgacata
    21661 ttcctggtga tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag
    21721 tttgtattct tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat
    21781 tttgaccaca tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac
    21841 atcttccggt gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac
    21901 ggggccgata accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg
    21961 ggcgcaggaa tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac
    22021 agcagaattt tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc
    22081 caccgccgcc ctcgccaaac cgacaccgat agacgcagca atttgagcca cggtaacggg
    22141 ggattcgtat tggcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca
    22201 aaacattaaa aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt
    22261 cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc
    22321 aaagaatttt tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcatttctt
    22381 tatgcaacat tgtggcaaga tttggggatg tcgtctgaaa aaccgtggga attcatttat
    22441 aatgccgtct tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc
    22501 ttaaatttac cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc
    22561 acggcaaaat tgaaacgccg gtatttatgc ctgtcggtac ttacggctcg gttaaggcga
    22621 tgaacccgca aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt
    22681 tgtggctgcg tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg
    22741 gctgggacaa accgattctg accgactcgg gcggttttca ggttttttct ttgtcggata
    22801 tgcgcaagct gacggaagaa ggctgtacgt tcaaaagccc gatcaacggc gacaagctgt
    22861 ttttatcgcc tgaaatttcc atgaaaatcc aaacggtttt gaactcggac atcgcgatgc
    22921 agctggacga atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgttgcaaa
    22981 tgagcctgcg ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg
    23041 cactgttcgg catcgtgcaa ggcgcgatgt atgaagattt gcgcgaagag tcgctgaaag
    23101 gcttggaaga gctcgatttc cccggcttgg ccatcggcgg cttgtccgtt ggcgaaccta
    23161 agcccgaaat gtaccgcatg ttgcgcgccg tcggccctat cctgccggaa cacaaacctc
    23221 attatttaat gggcgtcggt acgcctgaag acttagtgta cggcgtggca cacggcgtgg
    23281 atatgttcga ctgcgtcatg cctactcgca atgcgcgtaa cggctggctg tttacccgtt
    23341 ttggcgattt gaagatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa
    23401 gctgcacctg ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg
    23461 ccggcgaaat cttgggcgca cagctcaaca ccatccacaa cctgcatttc taccaagtca
    23521 ttatggcgga aatgcgcgat gccatagaac aaggcaaatt tgccgactgg caggcgcgat
    23581 tccacgaaaa ccgtgctcgc ggcacagact gactcccggc aatgccgtct gaagccgctt
    23641 cagacggcat tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc
    23701 cattctttcc agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt
    23761 tgaacctcgg cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct
    23821 ttctgaccga ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg
    23881 gtctggtact gacgttgcgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta
    23941 aggaaggttc gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta
    24001 tgcccgacct catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat
    24061 tgtcggaact gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat
    24121 atgccgcttc cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac
    24181 ggaaccaaat gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg
    24241 cgatgaaaaa ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt
    24301 tgagttttca cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt
    24361 gccgccacac cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg
    24421 tatcgttcca aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt
    24481 tcggcaaact gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg
    24541 cagactgttt ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttacgaag
    24601 caggcggcaa aagctaccgc tacatcccct gcctcaacga caaccccgac tggatagacg
    24661 cactcgtcgc acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg
    24721 tctaaaatgc cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga
    24781 tggcatctat gccggacaac aacatcaagt gcaaccttac taccgattcg gcaaaaaaat
    24841 ctttcacccg ctacgcaaaa tcaacaacaa ggtttcaaac attaaggaaa caccaatgaa
    24901 cacaacccga ctgccgaccg ccctcgtctt gggctgcctc tgcgccgccg cttctgccgc
    24961 cgacaacagc atcatgacaa aagggcaaaa agtgtacgaa tccaactgcg tcgcctgcca
    25021 cggcaaaaag ggcgaaggcc gcggaaccat gtttccgccg ctctaccgct ccgacttcat
    25081 catgaaaaaa ccgcaggtgc tgctgcacag catggtcaaa ggcatcaacg gtacaatcaa
    25141 agtcaacggc aaaacctaca acggattcat gcccgcaacc gccatcagcg atgcggacat
    25201 tgccgccgtc gccacttata tcatgaacgc ctttgacaac ggcggcggaa gcgttaccga
    25261 aaaagacgta aaacaggcaa aaggcaaaaa aaactaaaca gacaaaatgc cgtctgaaac
    25321 cggcaatccg gcttcagacg gcattcaaat caaacctttc taacgcggta ctttcagccc
    25381 catagacccg atattgccgt ccccgtcaac ctccttcgcg gtcagcataa agccgccgat
    25441 gtcaatacgg ccgcactcga ccggctcctg actgtcggaa cgcttatcga aaagctcggc
    25501 aaggctcaaa ccctcttcgc ccgcttccag cctcaaaccg taagcaagtg ccaaatcacc
    25561 cgaacgtgct gccggcaaaa cgacaaactc accgaagaaa tcaaaatact cacggacgct
    25621 aatacccgtc tcggtaaagt atttcgccat tttatagtga actaaattta aaccagtacg
    25681 gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa
    25741 tcggttccgt actatttgta ctgtctgcgg ctcgccgcct tgtcctgatt tttgttaatc
    25801 cactatatcg accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg
    25861 gtgtcggact gcatttcgat tcggctatac gccttttcac gcaggctgta attgaatttc
    25921 cacaaaccgc cgaacactgc cggcggacgc gcgaccagca tcaggaacgc cgcaatcgcc
    25981 aaggcttccg ccgccccgtc caacacgccg gcgggagaaa ccagcagcaa aggcattccc
    26041 aaacgtgcgg gtaaagtggt cgaaaccacg ctcagaaaca acagtgcgcc acccggcagg
    26101 aacagactgc ccatccaatc cacgctaacg attctccctt aaaattcatg aaattaaaat
    26161 cgaaagactg taacacggca ttttcttttc acgaaacttt acttcacgtc cgccacagcg
    26221 ttcagacggc attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat
    26281 ataatgtcgg actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta
    26341 ccctgccgga cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg
    26401 caaagaaaac cgccggcgtg gtcggcgacg atttggcacc caatgccaac cgggttacgg
    26461 gcgtatcggc agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca
    26521 acaagccggt tttggtgcct gcggccctgc tgctttccgc cttcctgccg caactcatta
    26581 cgccgctttt gacggcaggc ggcatttatc tgtgctttga cggcgtagaa aagctgctgc
    26641 acaagtttct gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg
    26701 actgttttgg tgtacggaat cgttgccgtc atcgtcaaac tcgacaactt aggcatgctg
    26761 ctgatgcgcc gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg
    26821 ccttgcttta tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tgtcggcggc
    26881 gtactggtcg cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac
    26941 gctggctggg cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc
    27001 tgcgcccccg ccttgccgct gatgaatcgt ttcggcaggc attgatttct ttttcaaata
    27061 ccgatgccgt ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatgtttt
    27121 ccaaactgga caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg
    27181 ccgccgccac cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt
    27241 tcggtgcctt cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc
    27301 tgttcagcct gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg
    27361 tttccggcct gcccgacctc tatgccgtac cgctgacttt cctactcccc gcctaccttg
    27421 ccctttatcc ggcactgtgt ttctggctgt ggaaaaaatt taccctgcct cggggcataa
    27481 aaatcggttt ggtactgccc atcctgtgga cactgaccga gtttgcccgc gaacgtttcc
    27541 tgaccggatt cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg
    27601 ccggctttgc cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg
    27661 tctggctggt tttggcgagt gataacaccg cacgttcggg caaacgcctg cttccaatta
    27721 tcctgattgc cgccctgctt gccgcaggct acaccgcccg acaaaccgac ttcacccgcc
    27781 ccgacggcag ccgcagcacc gtcgccctgc ttcaaggcaa catcgaccaa accctcaaat
    27841 ggcgtgaaga ccaagtcatc ccgaccatac agaaatatta cgaacaggtc ggcaaaacca
    27901 ctgccgacat cgtcatcctg cccgaaaccg ccatccccgt tatgcgccaa aacctgccgg
    27961 aaaacatact ggcaaaattt gccgaacagg cgcaaaacaa cggcagcgcg ctcgccgtcg
    28021 gcatcagcca atacacttcg gacggcaacg gttacgaaaa cgccgtcatc aacctgaccg
    28081 gttatcagga aaacaatcag gacggcatcc cctactacgc caaaaaccac ctcgtcccct
    28141 tcggcgaata caaaccgctg cccttcctga ccacgccgct ttacaaaatg atgaatatgc
    28201 ccctttccga cttccgcaaa ggcggcggta agcaatccgc cctgctgatg aaaaaccaaa
    28261 aaatcgcctt caacatctgt tacgaagacg gattcggcga cgaactgatt gccgccgcca
    28321 aagatgccac cctgcttgcc aatgccagca atatggcgtg gtacggaaaa tccaacgcca
    28381 tgtaccagca cctccaacaa tcgcaggcgc gggctatgga actcggacgc tatatggtcc
    28441 gtgccaccaa caccggcgca accgccatca tctcccccaa aggcaacatc atcgcccaag
    28501 cccaacccga tacggaaacc gtattggaag gacacatcaa aggctatgtc ggcgaaacgc
    28561 cctatatgaa aaccggcagt tcatggtggt tgatgggcat attgacccta gccgcactga
    28621 ttcttttcat cttccgaaac aaagaacact gatacctgca cagcccgtcc ggacgggctg
    28681 tcttttttca aaacggctat ttacatacaa ttacgtatgt aatataattc tgaaatcgaa
    28741 ttgagccatc aggtttacaa aatcaatcaa acctgcctta caaaattttc ctgtctttca
    28801 aaccttagcg catatctcct gccaaacata caaaatacaa gccaagcagt gatatactgc
    28861 atcccaacac agcaaaccgc cgatacgcag tttcagccga aaggaaatgc cccaaatgaa
    28921 taacgctttc gcattacccg ccatccaaag cggcaacggc agcctcgaac aatacatcca
    28981 caccgtcaac agcatcccga tgctgtccca agaagaagaa acccgcctcg ccgaacgccg
    29041 gataaagggc gacctcaacg ccgccaaaca actcatcctg tcccacctgc gcgtcgtcgt
    29101 ttccatcgcg cgcggctatg acggctacgg gctgaatcag gcagacctga ttcaggaagg
    29161 caatatcgga ctgatgaagg cggtcaaacg ctacgagcca ggtcgaggcg cgcgtctgtt
    29221 ttcatttgcc gtacactgga ttaaagccga aatccacgag tttattttga gaaactggcg
    29281 tttggttcgc gttgccacca ccaaaccgca acgcaagctg tttttcaatt tacgcagtat
    29341 gcgtaaaaac ctcaacgccc tgtctcccaa agaggcacaa gacatcgccg acgatttggg
    29401 tgtcaaactt tccgaagttc tggaaatgga acagcgcatg acgggacacg acatcgccat
    29461 catggcagac aacagcgacg acgaggacag tttcgccccc atcgactggc ttgccgacca
    29521 cgattccgaa ccgagccgac aactatcgaa acaggcgcat tacgccctgc aaacagaagg
    29581 tttacaaaac gctttggcgc aactggatga caggagccgc cgtattgtgg aaagccgctg
    29641 gcttcaagat gacggcggtc tgacgctgca ccagcttgct gcagaatacg gcgtctctgc
    29701 cgagcgcatc cgccagattg aagcgaaagc catgcagaaa ctgcgtggtt tcctgaccga
    29761 agaagccgaa gcggtttgaa atatacgcaa taaaataccc gacaaattgt tttgtcgggt
    29821 attttatttg gtaaacatca atgcggtttg tgcgtcgtgc cgtacacgat tttatccgtc
    29881 caagccatcg caatcgccga aatcaaaaag cagacatgca ccagacactg ccgcatcagc
    29941 tgcttttccg acagattggc gaaattgata tagtggatta acaaaaatca ggacaaggcg
    30001 gcgaagccgc agacagtaca aatcgtacgg aaccgattcg cttggtgctt cagcacctta
    30061 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggttttgg ttaatccact
    30121 atacatttcc cttccttgca gcaacaaaag cgcgtgattc caatctgccg ctgcaaaatc
    30181 ttcaattttt cacacccgaa aacgcttgcc gccatacaga tgtgatatat tgattccatt
    30241 ttaacgggct tcggagaacg acatgagcag cggaaatatc cacatccaca gcatgaccgg
    30301 atttgccaac gcggcggcag agtgcggcag caaacgcatc aatctcgaca tccgcgccgt
    30361 caaccaccgc tttttggaca ttcaaatcag gatgccggat gatttgcgct atttggaaag
    30421 cggtatccgc gagaaaattt catcccatat cgcgcgcggc aaagtcgaat gcaaaatcca
    30481 aattcaagat acggaaaaca gcagacagtc cttggaactc aactcggatt tggtgaacca
    30541 gcttgccgaa atcaacaaaa acctccgcaa gcatcacgat ttggcaaaac tgggcgttgc
    30601 cgacatcctg cgtttccccg gcgtattggc aagccaaagg gaaaatacgg aggaattggc
    30661 aaaaagcatt accgaactga ccgaaaaagc gttaaaagac tttactgccg cacgccggcg
    30721 cgaagggaaa aaattgggcg agcacctgtt acaacgcctc gaaagcatgg aagaaatcat
    30781 agatgcattg agcgaactct ttcctaccct gctggaaaca cataaagaaa aaatccgcgc
    30841 ccgcctcgtc gaagcagtcg gcgacatcaa taatgacagg ctgcaacagg aatttgccct
    30901 ctttattcaa aaatccgaca tcgacgaaga attcagccgc ctgcgcacgc atatcgacga
    30961 agtacgccgc atcgttaccg aacacaaagg cagcagcgga aaacgcttgg atttcctgat
    31021 gcaggaatta aaccgcgaag ccaacacttt gggcagcaaa tccattgccg ccgaatgcac
    31081 ccaagcctcg gtcgaactga aagtcttaat cgagcagatg cgggaacaag tgcagaatat
    31141 cgaataatcg gtttccccga taaccaaatg ccgtctgaaa gccctttcag acggcatttt
    31201 ttcaggttga aacctttgcg gtataaaggc ttaaggcttt cctttgaccg ccaccaatct
    31261 tgttcctgca agaaaatcat acagaaactg ccgatcggga ttcagcaggg caaaccccca
    31321 cggtaaaatc agccaaatca atgccgcacc gaatgcatcc ttgggtgaaa taccgagcag
    31381 gtggcgtaat ccggcatagg ctaacatagg aatgaagacg atgaatatgc acgcccaaat
    31441 aaagcgcagg cgcagcaggt acaaagacgg ctgtatgccg ttaaggtcgc acaagccgat
    31501 tttccatgtc ctcatcgcca aggtctgccc ctgaccgtgc cagttggcgc gaaaataaag
    31561 ccaccatgat cccattatca ggatacttgt taccaatgca gaaaccgcga tagaaacggg
    31621 attcagaaaa atggcggcaa taccggcaaa caatgctgcc aaacaggttg ccgcaccgac
    31681 cagcagcatt tcgtacagca tggcggcaaa gcggcgtttg agcggggcgg ttttcagtgt
    31741 catcatcttt tcaatcagaa tatgccgtct gaaacgccaa caggcttcag acggcattta
    31801 tgcaaagatg ccttgcggcg gttaacggtg cggacgtttc tgaccgcgcg actgttttgc
    31861 cgccatttga ttgatttcct gacgtgtcgg gcgttttttg aacatagcgt gcgcctcgtc
    31921 caccgaacat ttttgcgaca ggcagatcag ggtaatcgca tctttctggt gttgtccgcc
    31981 gctgtggtac atacggcgca ggtttgcctc gttgattctg cctttttcgg gatcgatgtc
    32041 gaattttttc aggcgttcga taaactggcg ttgctgtcgg cgggatgtcc aaacaaaacc
    32101 gcccgcagcg gcggcgacga tgacggcggc aagcaggatg gcaagcggcc aggagtcgac
    32161 aatcaatacg ataatgaggc ttaaaacggc ggtggtcagt aaaatcaggc cgcccaagcg
    32221 cagtttggtc ttattgtcca tagggttaat gttcctttat tgaggtggtc gtgccgtctg
    32281 aaatgttttc ggacggattg gtgaatactt ttcaggatta acggtatttt acagtatttt
    32341 cggattttgg ggattaccgc tttattccgc cagcgacacg acaagccgtt tgaataccgt
    32401 ccatgcgtcg ccctcttccg cgcctttgat gattcggtcg atttgggcac aggttttgag
    32461 cgcgtcaagc aggcggacga cggaaatccg cttgactgca agcggtgcga gcgtctgctt
    32521 gtcgccccaa agcctgaggc cgttgcggac ggattggatg ctctgcccct gcttcaaggc
    32581 ggcggcaagc cggatcagcg tccgcacgtc ttcggcaacc gcccacagaa gcagcaccgg
    32641 ttcttcgcct tcctcctgca atccgtccaa aagcctgcat acgcgcggga catcgccctt
    32701 catccatgcg ccggcaagct gaaaaacgtc aaagcgggca acattggcaa cggcggtttg
    32761 cgcctcatcg atattgacgg tatgcccttt cggatacagc agcgcgagct tgtcgatttc
    32821 ctgacgcgcc gccaacaaat tgccttccac gcgctcggca aacagtgcca atgcgtctgc
    32881 ctcgataccc aaaccgattt tgtccagccg tccgcgtatc cattggggca aggccgccgc
    32941 gccgaccggt ttggcttccc acacttcccc ctttgccgcc aatgcggcaa accatttgga
    33001 ctggagctgg gttttctcca gtttgggcag caaaaccagc gttaccgtat cttccggcag
    33061 gcgggcggca aaatcctgca acgcctcgcc gccggttttg ccgggttttc cgttggggat
    33121 atgcagttcc aacagcttca aatcggcaaa cagacccgca ctgcctgcgg tttgcaacag
    33181 ttcgttccaa tcgaaggcat tgtctgccgt ataaacttcc cgattaaggt aaccttgttt
    33241 cttcgccgcc gccctcaatg cgtccaatgc ctcgatacgc aacagttctt cctcgccgtg
    33301 gatgacgtac aggggtttca aaggcgcgtc cgtatcaatg tgtccgatat gtgccgccac
    33361 gtcattccgc cttcagaaag gtcaggcggc ggacaatctg ttcggcggca tcctgccgca
    33421 tttccgccca caatgccgct tcctcttcct gtttgcccaa gatctcgttg tcggcataag
    33481 caaggacgcg gcggacggac acggtcatcg gtttgccgac cggctcgccg cgtttcaata
    33541 cctgcgcttc aaccgtcaag attaaaagat attcgttgat aactgccgca cgggtaacgg
    33601 tgtaggtttc cttgttttgg gaaacgctgt ctatacgcag ggtcatctgc gcgccggcag
    33661 catcgtccac cctgcccgaa gcctgataca gcgcggtttc caaaggaaac tgcaatgcct
    33721 gtccgccttc gatgtgccag ctccggtagg tcagcggcgg agaaatgccg tctgcacctt
    33781 tcaggtggaa accgcacgcg cccagcatca gcactgcggc agtaagaaac agtttgttca
    33841 tggcttgcct ttctcaaaag cacaaagtcg gtagattata tcagcggcgt gttcagacgg
    33901 cattgccgtc cagccaaatc aggctcgcca tacgccctgt cgctccgtcg cggcggtagg
    33961 aaaagaaagt gtcccgttcc aaaaccgtac aatgcgtgcc gccatatacg ccgcccacgc
    34021 cttcacgctt cagaatcagg cgggcgagcg cgtaaaggtc ggcaaggaat ttgccgccgc
    34081 ctatgccttc aaatgcggcg gcggcttcgg gcatgggcgt gcagaacgca tcaaacacat
    34141 cctgtccgac ttcaaacgca tccgcactga tggcggggcc gagatacgcc atcatttcga
    34201 caggcggaac cttcatcgca gcaatggtgt tttgcaatac gccgcccgcc aaaccgcgcc
    34261 agcccgcgtg tgcggcggca accgccgtac ccgccctgtc gcaaaataga acgggcagac
    34321 agtctgcggt catcacggca caggcggcct tgcccgtgtc gtccacggaa gcgtcggcat
    34381 cgggcgtgcc gcctaacgct tcggcagcgt tgacgacgat ggtgctgtgg atttgattga
    34441 ggtaggcaac gggcaatccg acctgctgtt gcacgatttc gcggttacgg cgcacggctt
    34501 cagggtcgtc gccgacgtgc gtaccgaggt tcaaactctg atacgcacct tggctcacgc
    34561 cgccgttgcg cgtggtaatc agggttttca cattggcggg cgcgggccaa tcggcggtca
    34621 ggaagttttt gcctttcggg gcgagattta gggtttctgt gatggttttc attggatatt
    34681 ggaataggtg tgtggcggaa agggttgatt aactgccttc ggggcaaatg gatgtttgtt
    34741 ttttcgtcat tcccgcacag gcgggaattc ggagattcgg gattgttttc aattgttcaa
    34801 acgttcggat tcggattctt gcaggtgcgg gaatgatgcc cttatatata gtggattaaa
    34861 tttaaatcag gacaaggcga cgaagccgca gacagtaca
//
LOCUS       DAXIHS010000012        33532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXIHS010000012 DAXIHS010000000
VERSION     DAXIHS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.012"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(188..934)
                     /locus_tag="ACFOF2_000490"
     CDS             complement(188..934)
                     /locus_tag="ACFOF2_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC7106864.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGMTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVPELSYVQKTITGRYSIYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(918..1097)
                     /locus_tag="ACFOF2_000491"
     CDS             complement(918..1097)
                     /locus_tag="ACFOF2_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106865.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(1113..1784)
                     /locus_tag="ACFOF2_000492"
     CDS             complement(1113..1784)
                     /locus_tag="ACFOF2_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC7106866.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1853..2524)
                     /gene="rpiA"
                     /locus_tag="ACFOF2_000493"
     CDS             complement(1853..2524)
                     /gene="rpiA"
                     /locus_tag="ACFOF2_000493"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC7106867.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2601..3083)
                     /gene="ispF"
                     /locus_tag="ACFOF2_000494"
     CDS             complement(2601..3083)
                     /gene="ispF"
                     /locus_tag="ACFOF2_000494"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC7106868.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDEDSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3115..3804)
                     /gene="ispD"
                     /locus_tag="ACFOF2_000495"
     CDS             complement(3115..3804)
                     /gene="ispD"
                     /locus_tag="ACFOF2_000495"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC7106869.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3801..4535)
                     /gene="dnaQ"
                     /locus_tag="ACFOF2_000496"
     CDS             complement(3801..4535)
                     /gene="dnaQ"
                     /locus_tag="ACFOF2_000496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC7106870.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(4693..6003)
                     /locus_tag="ACFOF2_000497"
     CDS             complement(4693..6003)
                     /locus_tag="ACFOF2_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC7106871.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5993..6178)
                     /gene="ccoS"
                     /locus_tag="ACFOF2_000498"
     CDS             complement(5993..6178)
                     /gene="ccoS"
                     /locus_tag="ACFOF2_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC7106872.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6178..6477)
                     /locus_tag="ACFOF2_000499"
     CDS             complement(6178..6477)
                     /locus_tag="ACFOF2_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106873.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6828..8024
                     /locus_tag="ACFOF2_000500"
     CDS             6828..8024
                     /locus_tag="ACFOF2_000500"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC7106874.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8660..10501)
                     /locus_tag="ACFOF2_000501"
     CDS             complement(8660..10501)
                     /locus_tag="ACFOF2_000501"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC7106875.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10592..11062
                     /locus_tag="ACFOF2_000502"
     CDS             10592..11062
                     /locus_tag="ACFOF2_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC7106876.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSVLEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11133..11945)
                     /gene="hpnC"
                     /locus_tag="ACFOF2_000503"
     CDS             complement(11133..11945)
                     /gene="hpnC"
                     /locus_tag="ACFOF2_000503"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC7106877.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(12032..12514)
                     /locus_tag="ACFOF2_000504"
     CDS             complement(12032..12514)
                     /locus_tag="ACFOF2_000504"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC7106878.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12987..13253
                     /locus_tag="ACFOF2_000505"
     CDS             12987..13253
                     /locus_tag="ACFOF2_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106879.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13363..14730
                     /locus_tag="ACFOF2_000506"
     CDS             13363..14730
                     /locus_tag="ACFOF2_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC7106880.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14911..16896)
                     /locus_tag="ACFOF2_000507"
     CDS             complement(14911..16896)
                     /locus_tag="ACFOF2_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC7106881.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFENSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFHYIEVTDNSHVIGQTISLDE
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKVS
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQWTF"
     gene            complement(17312..17758)
                     /gene="smpB"
                     /locus_tag="ACFOF2_000508"
     CDS             complement(17312..17758)
                     /gene="smpB"
                     /locus_tag="ACFOF2_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC7106882.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17813..18823)
                     /gene="waaF"
                     /locus_tag="ACFOF2_000509"
     CDS             complement(17813..18823)
                     /gene="waaF"
                     /locus_tag="ACFOF2_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC7106883.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18954..19029)
                     /locus_tag="ACFOF2_000510"
     tRNA            complement(18954..19029)
                     /locus_tag="ACFOF2_000510"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18994..18996),aa:Asn,seq:gtt)
     gene            complement(19220..19972)
                     /locus_tag="ACFOF2_000511"
     CDS             complement(19220..19972)
                     /locus_tag="ACFOF2_000511"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC7106884.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGGKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20283..20807
                     /locus_tag="ACFOF2_000512"
     CDS             20283..20807
                     /locus_tag="ACFOF2_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC7106885.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGDNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVRRRN
                     AASLQLSRCASIADDPSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSVSTAAA"
     gene            complement(21547..22692)
                     /gene="dapE"
                     /locus_tag="ACFOF2_000513"
     CDS             complement(21547..22692)
                     /gene="dapE"
                     /locus_tag="ACFOF2_000513"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC7106886.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22806..23426)
                     /locus_tag="ACFOF2_000514"
     CDS             complement(22806..23426)
                     /locus_tag="ACFOF2_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC7106887.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23460..23969)
                     /locus_tag="ACFOF2_000515"
     CDS             complement(23460..23969)
                     /locus_tag="ACFOF2_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC7106888.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(24064..24615)
                     /gene="azu"
                     /locus_tag="ACFOF2_000516"
     CDS             complement(24064..24615)
                     /gene="azu"
                     /locus_tag="ACFOF2_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC7106889.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(24896..25318)
                     /locus_tag="ACFOF2_000517"
     CDS             complement(24896..25318)
                     /locus_tag="ACFOF2_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC7106890.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25449..28199
                     /gene="secA"
                     /locus_tag="ACFOF2_000518"
     CDS             25449..28199
                     /gene="secA"
                     /locus_tag="ACFOF2_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC7106891.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28343..30115
                     /gene="dnaG"
                     /locus_tag="ACFOF2_000519"
     CDS             28343..30115
                     /gene="dnaG"
                     /locus_tag="ACFOF2_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC7106892.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30302..32230
                     /gene="rpoD"
                     /locus_tag="ACFOF2_000520"
     CDS             30302..32230
                     /gene="rpoD"
                     /locus_tag="ACFOF2_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC7106893.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            32377..33371
                     /locus_tag="ACFOF2_000521"
                     /pseudo
     CDS             32377..33371
                     /locus_tag="ACFOF2_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         8511 a         9513 c         7904 g         7604 t
ORIGIN      
        1 tgacggctca tacatcaccc caaaaaattg aaaccaaaca gatgaaattc cctcttgcgt
       61 gaggctgaca gatgccgtct gaaagacttt cagacggcat agggcgtttt ctctttgaat
      121 gtaaatttgc cacaaaaaag ccgcctcaaa tgaataccgg ggcagctttt tgttgatata
      181 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      241 agaaagtcgt catcaggaga taaatcaggg cgacggtgta aagcggttct tcataaatcg
      301 aataccggcc cgtaatcgtt ttctgaacat aggacaactc cggcacggca atgaccgaca
      361 gcagcgagct gtctttcaag agcgtgatga attcgctcgc caaaggcggc agcatacggc
      421 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataagtcata cccaaagaac
      481 gcgccgcctc catctgccct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      541 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      601 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      661 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      721 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      781 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      841 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      901 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      961 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
     1021 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
     1081 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1141 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1201 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1261 tcggcatagt cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1321 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1381 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1441 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1501 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1561 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1621 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1681 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1741 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1801 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1861 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1921 cgcgggcgaa tatgccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatca
     1981 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     2041 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     2101 aacgcgcgct ttcgacgact tccactggca gagggaattt gcccaaacgt gaaacatatt
     2161 tgctttcatc cgcaatgcag atgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2221 ggtgcgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2281 cgatatagac cgccaacccc gatacttcgt tcaaagaaac gacgggaata tcatattggg
     2341 caagcagttc gctggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2401 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2461 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2521 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgtcatctta acattttttt
     2581 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2641 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2701 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2761 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2821 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt cctcatcttt gaactctgcg
     2881 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2941 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     3001 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     3061 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg gtttgaattg aagttcagac
     3121 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3181 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3241 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3301 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3361 gtccgcgcgc ttgagcgtat cggcaacggg aattgccaaa atcccgcctt cggcggcgtt
     3421 gcccgcctgt tctatcaaac gcgtcagagc ttcagacggc aggcagcagc gcgcggcatc
     3481 gtgtaccaaa atattgtcgg tttccgccgc caaaccgatt tccaacagtt ttgccacacc
     3541 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3601 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3661 gacgccctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3721 ttcgacatac tgcttaggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3781 cagcgcgata tttttgcgct tcatgcgtcc gccccgccgt tttccgacgg cacggcttcc
     3841 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccagttcg
     3901 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3961 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     4021 atagcaagat agactttgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     4081 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4141 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcgacagtc
     4201 ggcagcccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4261 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4321 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4381 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4441 accatttcta aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4501 gtttcggtat cgaggatgat ttggcgttga atcatgtctg cttctttctt caaattttcc
     4561 atctgctgac tattttttaa gcaatatatt tttctgtttt catttcaatg cacaaaccca
     4621 cttattcaca gtgtgttcac aacattgggc aggcggattg tgtattttgg ggacaatttt
     4681 ttcagacggc attcaaggtt ttttcctgat tgccgccgcg cctaaaaacc gcctttcgcg
     4741 cttaatcaaa aataccgaca acggaatatt gcccaaagcg acaatcagat acaacaagga
     4801 aatgctgtca aacaaaaaca gcaacaccgc gctcaaaacg gcggcggaaa ccataaaaat
     4861 accgttaacg atattgttgg cggcaacggc gcgggcgcgg aaagtctcgc tactggcggt
     4921 ttgcagccaa gtatagagcg gaacggagaa aaatccgccg aaaaagccga tcagcgtcat
     4981 caccgccatc acgggatatg cccatccttg cgataaaaac caaaaaatgc cgttcagccc
     5041 ttcaaaacgg tgtccgtgcg tcagccacac caaaaccagg ccgcaaaccg tcaaacccaa
     5101 cgcaccaacc gttacccaag ccaacatcag ccgttccctg ctgaacttgg cacacagtac
     5161 cgaaccggcg gcaataccga tggaaaacag ggcaagcatc aggttgaaaa cattgtcgtt
     5221 gccgcccaaa tggatttggg taaaggtcgg cagttgcgta gtatagaccg cgccgacaaa
     5281 ccaaaaccac gaaataccga taatagcggt aaagacgggt ttgtaccgca ccgtttcacg
     5341 aagcagggat tttgtgccac ggacaatatt ccactcaatt tgggtatcgg cagccttggc
     5401 ggggacggac ggcataaaca ggctgccgac cgtgcctccg acggcgacca gcaaaaccag
     5461 tatcccgaca atataaggcg gtacacctgc caccgccgtt cccaaaatct gaccgaacag
     5521 gatggcgaca aacgtacccg attcaatcag gctgttgccc atcatcaact ctttgtcgtc
     5581 gagataatcg ggcaggatgg cgtatttcag cggcccgaac agcgtcgatt gcgcgcccat
     5641 gcaaaacaga cacgccaaaa gcagcggggc agaccggata taaaacccgt atgccgccac
     5701 cgccataatg atcatttcca gcaccttgac ccaacgcgcc aaaacggcct tgtcgaattt
     5761 gttacccaac tgccccgaca gcgaggaaaa caggaaatac ggcaaaataa acaacaacgc
     5821 gcccaagttc aacatctgtc cggcaggcag gaagccgttt tgccccaaac cgtaaaaccc
     5881 aatcatcaca aacagcgcgg ttttgaacac attgtcgttg aacgcgccga gaaactgcgt
     5941 agcgaaaaga ggtgcgaaac ggcggctttt aaccagtccc aaaccgcctt ttttagcgta
     6001 catcgttttc cctctctttt tcaatcagtt tacttgtcga atcatcatcc atcaggatgc
     6061 ggtgcgccgg cccttccaag tcgtcaaact gcccgttttt gcccgaccac caaaaaaacc
     6121 agccgatgac aaacgccaaa ataatgctga tgggcaccaa tataaacatg ctttccatca
     6181 catattccct gtcaaatcgt tcaaaacaaa agtctgcccc gacacggtca gatattcgtt
     6241 acgcaaagtt ccgacgggag cttcgtcaaa aaacagctcg atacggtctt tgaccacgcg
     6301 ccaatattgg ggaatttccg tctgaccgaa cggcgacagg acatgatttt ccattccgcc
     6361 ttcaagtttg acggcaaaac gcccgctttg cggccgtgct tccgattcgt cgtcggcaag
     6421 caggatgaaa aagcctatat gccgtcccga ttggtcatga atactgaaat aatgcataaa
     6481 tttcccaccc gccttttttc agacgacacc aactaaaaac agggcgaatg taccagtttg
     6541 gacgggaaga atgcaaagaa attctccctc ccccagccga aaacaccggc aaaccgcata
     6601 tccccctttt ttccgtcaaa atacccgact tccgccattt tcacgcaaac gcccgattaa
     6661 gccaagcaat tgcaaagatt ttttgttaga atagcctgct tcttttatca accgtttcag
     6721 acggccccac tactttcccg cctaggaagg caaaacggat tcggcacgaa tccggttagt
     6781 atccgtgtcc gattccaatg ccgtctgaaa ctttccggag taagaaaatg tcccaaaaat
     6841 tgatcttggt tttgaactgc ggcagctcgt ccctcaaagg cgcggtcctg gataacggca
     6901 gcggcgaagt cctgctcagc tgccttgccg aaaaactcaa cctgcccgat gcctacatca
     6961 cattcaaagt aaacggcgaa aaacacaaag tcgatctgtc cgcacatccc gaccacaccg
     7021 gcgcggtcga agccctgatg gaagaactca aagcccacgg cctcgacagc cgcatcggcg
     7081 ccatcggcca ccgcgtcgtc agcggcggcg aactgtacag cgaatccatc ctcgttgacg
     7141 acgaagtcat tgccggcatc gaaaaatgca tcccgctcgc ccccctgcac aaccccgccc
     7201 acctcttggg cctgcgtgcc gcgcaaagca ttttcaaagg cctgcccaac gtcgtcgtat
     7261 tcgatacctc cttccaccaa accatgcccg aagtcgccta caaatacgcc gttccgcagg
     7321 agttgtatga aaaatacggc ctgcgccgtt acggcgcgca cggtaccagc taccgcttcg
     7381 tcgccgacga aaccgcgcgc ttcctcggca aagacaaaaa agacctgcgt atggtcattg
     7441 cccacttggg caacggcgcg tccatcaccg ccgtcgccaa cggcgaatcg cgcgacacca
     7501 gtatgggcct gaccccgctg gaagggctgg taatgggtac gcgcagcggc gacatcgatc
     7561 cttccgtatt cggcttcctc gccgaaaacg ccaatatgac catcgcccaa atcactgaaa
     7621 tgctgaataa aaaatccggt ctgctcggca tttccggcct gtccaacgac tgccgcacca
     7681 ttgaagaaga agccgccaag gggcataaag gcgcgaaatt ggccttggat atgtttatct
     7741 accgccttgc caaatacatc ggcagtatgg cggttgccgc aggcggtttg gacgcactgg
     7801 tctttaccgg cggcatcggc gaaaactccg acatcatccg cgaacgcgtg atcggctact
     7861 tgggcttcct cggtctgaac atcgaccaag aagccaacct gaaggcacgc ttcggcaacg
     7921 ccggcgtgat taccactgcc ggcagcaaag ccgttgccgt ggtcattccg accaacgagg
     7981 aattgatgat tgcacacgat accgcccgct tgagcggtct gtaagattca atccccacgc
     8041 gaactgcctc cgaaaacgga ggcagttttt ttatccggct ttccatgctt aaacagcact
     8101 gcctcttttc agacattgac ggttgcagcc gcttacctga accttatagt ggattaacaa
     8161 aaaccagtac agcgttgcct cgccttagct caaagagaac gattctctaa ggtgctcaag
     8221 caccaagtaa atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     8281 tttttgttaa tccactatat ccgactttcc atacttaaac agcactgcct cttttcagac
     8341 attgacggtt gcagccgccc acctgaacct taatgattga ctatttttta atcatgttat
     8401 tattttccat aaaatacatg gcattaagat gtttttccac aaaagataca cacaccggca
     8461 aacaccggct gtgtttatct tttcttatgc ctatttttta atcatcgtat ttttatcttt
     8521 taatttcaat acgcaaacta acttatacac acggttttca catctttaga ctgcttccgt
     8581 gtgtatagtg gatattgccg ttttcctttc tgacaaaaat gccgtctgag aacttcagac
     8641 ggcatttgaa acatcggaat cagcggtttt gttcatacca ctcgataaac ttgtctgctt
     8701 tgacaaaacc cagcagcggc tcgctgcggc tgccgtcgga gcggacgaca aacacgcccg
     8761 gcggcccgaa cagaccgtat tctttcaaca acgcctgatg ttcaggcgtg ttggcggtta
     8821 cgtcgatttg gaaaaagcgt tccatatcaa ccgcctgatg cacttccggc tgattgagcg
     8881 tgtatgccgc catttctttg caggaaatac accagtcggc ataaaaatcc aaaacgacgg
     8941 gtttgtcggg atgttctttc aacgccgtat ccatcgctgc cttcagcgcg gcagtatcgg
     9001 caaacatttt gccgtgttcc gaagatttgc ctgcttcggc tggtggattg agggtcagga
     9061 aatggtgcag cgcggtcgtt ttgccgtttg cgccctgcca gccgaaccac gcgccgccta
     9121 tcagcaatat gccgcccaat gcgaatgcca cagctttcgg acggcgtttc tgcctgcgtc
     9181 cgttgaccag cagcatacag gcaggaacca gcatcagcag cgtgtacagc gcgacgacga
     9241 gataataggg caagtgcggc gtggcgaggt aaacggcgac ggcaagcagg ataaagccga
     9301 aagcgtattt gacggcattc atccaatcgc ctgccctagg caggatatgc ccgccgaacg
     9361 tgccgatggc aatcagcgga acgccggtgc ccaacgccaa agtgtaaagt gccaaaccgc
     9421 ctaaaaccgc atcgcccgtc tgaccgatgt agcccaaagc aaatgccagc ggcggggcga
     9481 cgcacggccc gacaatcagc gcggacaata tgcccataat aaagacggaa acgattttac
     9541 cgcctgaaag cctgctgctc tggttttgga aatacgactg cacggcgttg ggaagctgga
     9601 tgttgaacag cccgaacata gacagtgcca agacgaccat taaagccgat gccgccaaca
     9661 ccacccaagc ctgctgcaac catacggtca gcagcgcgcc cgtcagtccg gcaacaatgc
     9721 cgaccagcgt ataagtcaga gccaaaccct gaacataaac gacggacagc acaaacgccc
     9781 gcgccttgcc cgcctttttg tcgccgacca caatactgga aacaatcggc aacaggggat
     9841 acatacaggc ggtaaaactc agacccaaac cggcgagaaa aaacgccaaa agattggcgt
     9901 tgagcgtatc ccaagacagc ttgaaacggc tgtcgccgcc ctcatccccc ttcgggggcg
     9961 gcaacgcccc gctgccgttt tgagaggaag gctgcaaaaa gcggtctttg gcggatgccg
    10021 gttcgtcggt ttgcggatgg taagtgccgt tgccggaaat atcaaactcg gtatccacgg
    10081 gcggatagca cacgccggct tcggcacagc cctgataggt caaaaccaat ttatacggtt
    10141 cgccgacagc ctttgcataa ggaaaggcaa cctgcgcttc gtgatggtaa accgtctgcc
    10201 tgccgaaaaa ctcgtcttcc ttctcttcgc ccttgctgaa agaaggctgt cccaacaaat
    10261 ccgccggatc ggtcttgccg acgattttcg cctgatacat atagtatccg tcggcaatcc
    10321 tgaaacggac gttcacaccg tcgtcggcaa cggcaagctc cggcacgaat gccttttccg
    10381 gcggcagcag atcgttcgca tccagcgcga aagctcgtcc gcacaacatc aaaaatacgg
    10441 cgaacaggca aatcagtttt ttcataatcg aatccgtttc agacaaataa tttgtctgca
    10501 ttataaatgg taaggttgac ggtgggattt aatttatgta aaacccgcca ttatccgaac
    10561 ctatttccat aaacatctta ccgaacccgc catgtacgat gtcaataccc acgatgtccg
    10621 ccgctttttc gcccgcgtgt ggcagcagcg gctcaatccg ctgcaactga gcgtactgga
    10681 acagaaagcc ctccgcatta tcgaagccca tcccgaatac caccgttatc tcgaacgcat
    10741 cgaagaccat ctggacaccg actggctacc cgaaaacggc gaaagcaacc ccttcctgca
    10801 tatgtcgctg catttgtccg tccaagaaca ggcgggcata gaccagccgc acggcatacg
    10861 cgcaatccac gacaccctgt gcgccaaacg cggctggctg gaagccgaac acgaaatgat
    10921 ggaggcactg gcggaaacac tgtggacggc gcaacgctac ggcaccggtt tggatgtcaa
    10981 tttctacatg acccgactgc gcaaactcat cggcttgggt gcagaagatc aagccaggtt
    11041 gaacccgcat gaaatcgcct gaccacacca accgcctgca aaatgccatc tgaagcggaa
    11101 caaccccttt cagacggcat gcatttcccc catcatttcc acaacgcgcg tttcagcata
    11161 atcaaccaat ccttcttatc caaaacgggg cgttgtgcaa acacatcgta tcggcacgcg
    11221 tccagtttct gcaaaatcaa ctgcgccccc aacacaatca tacggagttc caaaccgata
    11281 cgccctttca attcgcgcgc taaaggcgaa cccgccttca gcatacgaaa tgcgcgccgg
    11341 cactcatacg ccatcagccg ctgaaacgcc gcatccgccc taccctccgc aatctgtcct
    11401 tcggaaacac cgaatttcaa caaatcgtcc tgcggaatat aaaccctgcc cttttgccaa
    11461 tccacagcca catcctgcca aaaattcacc agttgcaaag ccgtacagat gccgtcgctt
    11521 tgcgccacgc acaccgcatc cgttttcccg tacaaagcca gcataatgcg tccgacaggg
    11581 ttggcggaac gccggcagta atcggccagt tcgccgaaat gcgcgtaccg cgttttaacc
    11641 acgtcctgcg aaaacgccga aagcaaatca taaaacggct gcaaatccaa accgaacggc
    11701 acaaccgcct cggcatccaa tcgtgcaatc aaaggatgcg ccgaccggcc gcccgatgcc
    11761 aacacgtcca actcgcgccg caaagcatcc aaccccgcca acctggcttc agacggcata
    11821 ctgccctcgt ccgccatatc gtccgccgtc cgtgcaaacg catacaccgc atgaaccggc
    11881 ttcctcaacc tgcgcggcaa aatcagcgaa ccgacgggaa aattttcata atgcccaacc
    11941 gacatacctt ctccatccat caaacaaaat gccgtctgaa acggaacaaa cccttttcag
    12001 acggcatcag atacctccaa gctgccggca atcagtggtg gtgatgaccg tgcgggccgt
    12061 ggacatgacc gtgtgcgatt tcctcatcgg atgcatcgcg cacgctttca accgtagcct
    12121 taaaacggat tttcatgcct gccaaaggat ggttgccgtc caccaccgcc ttgccgtcgg
    12181 caacatcggt tacacgatag acgacaacat cgccggtttc aggatcgtcg gcttcaaaca
    12241 tcatgccgac ttcgacttca acagggaaca cgcccacatc ttcaatacga accaactccg
    12301 aatcctgctc gccgaacgca tcgtcgggcg aaagcgccac atcgaccgta tcgccggcat
    12361 ccttaccgtg caacgcctct tccaccaaag ggaaaatgcc gtcgtaaccg ccgtgcagat
    12421 acgcaatcgg ttcttcggtt ttgtccaaaa gctgattgtt ggcatcatac atctcataat
    12481 gcagcgaaac cacggaattc ttcacgatag ccatatttgt cctttcagga acagcagatt
    12541 gattacaggc gcattctaac acaaccgccg cgccggccga ttaccgttaa cctgttcata
    12601 aactgtacag cacatatttc aatgtaaatt tttgttattt tattgcgatg taactttttt
    12661 acaacattat taaaaccatt ccgacctgtc tgccgacttt cccaatccgc cttaataaat
    12721 catacaagat actgaaatta tattaatctc tataatattt atccctatcg aatttttaac
    12781 agcaaaaccg ttttacagga tttatcaatc cgcccgccag aaaacttttc attcaaacct
    12841 ttttcccatc tgtacgacat tgcaatccct tattccatag tgcataatta cgcaaattca
    12901 gcgatgaatt tccaacccgg tttgtagtat ggtcgataaa gacctatttg tttcaataat
    12961 ttaaattggt tctaaaggtt actaaaatga aaaaatccct gtttgccgct gctttgttgt
    13021 ctttggtcct ggcagcctgc ggcggtgaaa aagccgctga agctcccgct accgaagcac
    13081 ctgccgccga agctcccgct gctgaagctg ccgctactga agcacctgcc gccgaagctc
    13141 ccactgctga agctgccgct actgaagcac ctgccgccga agctcccgct gctgaagctg
    13201 ccgctaccga agcacctgcc gctgaagctc ctgctgccga agctgcaaaa taagcatttt
    13261 ccgcttgcaa aaaagcagga tacgttcagt atcctgcttt tttgattttt cagacggcat
    13321 cagattccct tcctcaatct tctccctacc cttccgacaa acatgcctga ccttcatacc
    13381 gaattttccc gactcctgcc ggcagatgaa attgccgaac tttctccgac gcttttaaaa
    13441 gaccagcgca accgctttac gtctgcacca gacatcattt tgcagccgcg cagcgttgaa
    13501 agcgtgcaaa ccattatgcg tttttgttac gaacaccgta ttccggttac gccgcaaggc
    13561 ggcaataccg gtttgtgcgg cgcggcagta tcggaaaacg gcgtattgct gaacctttcc
    13621 aaactcaacc gcatccgcag catcaatttg tcagacaact gcataaccgt cgaagcaggt
    13681 tccgtactcc aaaccgtcca acaggcagcc gaagcctcaa acaggctgtt cccactcagt
    13741 ctcgccagcg aaggctcctg tcaaatcggc ggcaacatcg cctgcaatgc cggaggtttg
    13801 aacgtattgc gttacggcac gatgcgcgac ctgattatcg gtttggaagt cgtcctcccc
    13861 aacggcgagc tggtttccca tctccatccc ctgcataaaa acaccaccgg ctacgacctg
    13921 cgccatctgt ttatcggcag cgaaggcaca ctgggcatca tcactgccgc cacactcaag
    13981 ctttttgccc gtccctcgga caaggcaacg gcatgggtcg gcatacccga catcgaatcc
    14041 gccgtccgcc tgctgaccga aacccaagct cactttgccg aacgcctatg cagttttgag
    14101 ctgatcggcc gttttgccgc cgagttgtct tccgaattca gcaaattccc cctgccgaca
    14161 cattcagaat ggcatatttt acttgagttg accgactcat tacccgacag caatcttgat
    14221 gatcggctcg tcgaatttct ttataaaaaa ggatttaccg acagcgtatt ggcacaaagc
    14281 gaacaagaac gtatccatat gtgggcattg cgcgaaaaca tctccgcctc ccaacgcaaa
    14341 ctgggcacca gcatcaaaca cgatattgcc gttcctatcg gacgcgttgc cgactttgtc
    14401 cgccggtgcg ccaaagattt ggaacagaat tttaaaggca tacaaatcgt ctgcttcgga
    14461 catcttggcg acggcagcct gcactacaat actttcctgc ccgaaatcct cagcaatgaa
    14521 gtctatcgtt acgaaaacga catcaacgac gcagtttatc gcaacgttct tgcctgcaac
    14581 ggcacgattg ccgccgaaca cggcataggt atcatcaaaa aacagtggct ggacaaagta
    14641 cgcacgcctg ccgaaatcgc cctgatgaaa agcatcaaac aacaccttga tccatataac
    14701 attatgaatc cgggcaaact gcttccgtaa ccggcatttc tgatttgcat acacaacaaa
    14761 gaaagggaca atagatccga ttgtcggttt agcgcgagct cgtgagtgcg gtaaaaaatt
    14821 ggtggaaatg acacgaaaaa tgaccgcatt tttaaaataa aaaaatcggc agtgaatttc
    14881 cctgccgatt ttattttgtt acaacttaac ttaaaacgtc cactgcaaat tcaacgcacc
    14941 ttgtttagcc tgatgatatt tgcttgtttg gcggttgaat gaggcttgta aagttaagtg
    15001 agatttgatt ttcactgcta cacctaattg gctctcaatt gccgtcttat tgtttatcac
    15061 tcgacgctct ccgtctattt ccacaccgaa aggtttgttg tggtaaagcg cgttcacagc
    15121 ggcgaaaggt tcaatagcga tatttttata gagtgaaaat tgagctttag cttgaacgcc
    15181 aacccgagtt tgtaattggc gggagccaag taaattcacg tgggcatttt cgctatcgct
    15241 gaattttccg tttaccccca aataagtcaa ttgtgcctgt ggttgtaggt aaacacgaag
    15301 gctgttgccc tttttagtga agtgttctgc caataaagca ttgtaacccg cttcaattga
    15361 ggcggtaata ccttttgaag taaaacgttc tgtagcatct tcagtgttaa tgcggtggcg
    15421 gaagcgttga tattgtaccc agctatccgc atacgcacct gtctgtttgt cctgaagctg
    15481 atgccaagtg gcataaatgc ccgccccaaa gcctttcaca tttcccgttg taagattgtc
    15541 tgtatctgga ttgcggaaag tgctgcgttg ttctgcttgt ccgctcatta agccaacgga
    15601 aagttgatta ctttcgtttt gccatgtgaa tacttcgcca ccgagttgca cacctttacg
    15661 atagccttct acaggtgctg ttttgccttg cacccattgg ttggaatgtc cgtcaatcac
    15721 acgcaaccac aagcctttgc gtggtaaagt gcggtcaaaa atatcgctgt ttttgtcgtt
    15781 caaacgcaag gcgaataagg tattggcggc ttgagcctgt tgtgcataaa tcgaaatatc
    15841 atcgcgttct tgcactttgg taaaaaagcc ttctgggcgt tgttgtaaag aaagcgtata
    15901 aattcccttt tggtgtttgc cagaaagacg gaatgcgtgt ttatctgctg tgccatttac
    15961 tttgataatt tgatgcccgt cgagactttt taaatcgtct attggatttt cgaagatgat
    16021 gtcggaagtg ccagttatat ttttttcaaa agttaaaact ggtgtttcag agactttatc
    16081 ttttagatcg taagcaaaac gaaaacgagc tccgccagca taatcttctt ttacgagtaa
    16141 actttcactt ttagtattaa aacggatgtc tgcattcgtt gtttttaatt ccccaacatc
    16201 agaatcccaa cggggttccc agagagaatt ttctaagcgg aattcatcca aactaattgt
    16261 ttgtccgata acgtgtgagt tgtctgtaac ctcaatatag tggaatggat ctaaaccaga
    16321 atagagatgc tcttgccaac taggaagctt attaagtgtt gggcgaagcc attcgatata
    16381 attacctaca gataaaattt cgctgttgat atggctattt tttatttgtg gagctaaggc
    16441 gaaaatatcg ctctttatta atcttgggcc agagttttcg aaggatggtt gttcacactt
    16501 atcattattg gaattcccag tatttacaca gtccttaaaa tattgctgga gtgttttttc
    16561 ttcttcccca tttctttttt caaccccgaa acctcgggcg aagccaacaa ggtaatcttc
    16621 agtgtattcc ttgtcataaa aagaaatctt ttttgagttg ttgatgtttt tgaattggtg
    16681 tgttttagag tatagtgcgg gaactttgag agagttctga gagctaccat tcgtcggttt
    16741 tagaagataa gtttcttgtt tccagtattc gctgctttta tctctaagtt tttttaagcg
    16801 atcaatttgc tcatttgtta atttagaatt gtcgtaaacg taatcaacag ccaaaagcgg
    16861 agaggtataa agaatagaaa aaaatagact tataataaat gattttttta aacttctgct
    16921 tgcttgcttc gagtttcata ataaattttc ctttgtcaag taaaaataaa tggggcgtgg
    16981 attttagcat aaaactgaac aaaaaatgtc atttagctca catttttctc tatttatttc
    17041 ttatttatta aaggtaaacg tttgcttttt gctattttgt caagccagtt tgaaaatgtg
    17101 tataattgcc ctcgttattt acaaaaattt caggaaaaat gaccgcactt tacccttggc
    17161 taatgccaat ttatcatcaa attgctcaaa cctttgacga aggtttgggg catcatgctg
    17221 tgctgattaa agcggatgct ggtttaggtg tagaacgttt acacatcagg cggcagcctt
    17281 gcccataccc tctgaagcac tgtttccaca atcagcgcgt atgcttaatc aaccgctgtt
    17341 tctcgcgttt ccaatccgcc tctttcatac tctgacgttt gtcgtgctgt ttcttacctt
    17401 ttgccaaacc gatttccatc ttgatttttc cgcgcgaaaa atgcaaatcc agcggcacga
    17461 tggtgtagcc ggcacgttcg gttttgccga ttaatttgtt gatttccgac tggttcaaca
    17521 agagcttgcg cggacgtacg gcatctggtt taatgtgtgt cgaggctgtg ggcaaagccg
    17581 taatatggca gccgaccaga taaaacgcgt cttttttcca atagatataa ctctctttaa
    17641 gctgtacgcg cgcggcgcgg attgctttga cttcccagcc ttccaagacc aaaccggctt
    17701 caatccggtc ttcaatgaaa aaatcgtgaa atgctttttt attgttcgca atagccataa
    17761 acatcctatc aatatccgcc gtcagacggc ataaacccga aaacagaacc catcataccg
    17821 cctcttcaac cgcctgcaca atcttctcgg gatacagcct gttgaggcag tcggtatgcc
    17881 ccagcggaca ttcccgctta aaacagggcg aacattccaa gtgcaggctg acgattttcg
    17941 ccctgtcgct caaaggcggc gtatgcgtcg ggctggaaga accgtaaacc gccaccacct
    18001 tcctgcccaa tgccgccgcc aagtgcatca gcccgctgtc gttgcacaca accgtgtccg
    18061 ccaacgacag caaatccatt gcctgcgaca aatcggtttt gccgcacaaa ttgacacaca
    18121 taccgtctga aaggcagttg atttcctcgg caatttcatc atctttttgc gaaccgaaca
    18181 gccaaacctg ccaacccgcc gccagataat gtttgcccaa ctcggcaaaa tgccttgtcg
    18241 gccaacgctt tgccggcccg aattccgcgc ccgggcaaaa tgccaaaaca ggctttccaa
    18301 gattcaaacc gaaagtttcg acagaaattt cccgccgccg ttcatcaatg gaaaactcgg
    18361 ggaatcccga atgcccgtca aaatcttcct gactcggatg cgcgagagcc gtatatctgt
    18421 ccaccatcaa aggcagacgt tccttatcca gcctgcgtat atcgttcaac agaaaataac
    18481 ggctttcacc gacataaccc gtccttttgg caatacccgt tgctagcgcg atgactgccg
    18541 atttcaaaga accgggcaac acgataacct gatcgtatcc gcgccgcccc aaatccctgc
    18601 cgacccgcca gcggcgtttc aactccaacg caccgtgtcc gaacggattc tcaagaattt
    18661 cattcacttc cggcatacgc tcgaacaccg ccatcgacca cttcggcgcg aacacatcaa
    18721 tcgtgcaacc ggggtgaagt tccttcaaac ggcggaacaa gggctgggtc atcacgcagt
    18781 cgcctatcca actgggagaa ataatcagga ttttgatgga cataacaaga aaccgaaatc
    18841 agacaggcag aattttaccg cgaaaccgtt ggaaaaccta tcttgtctca ttccgaacgc
    18901 cgtacgtgca aatatgaaaa agcccgaaca ttcaagttcg ggcttcaaaa ttctggctcc
    18961 ccgacctggg ctcgaaccag ggacctgcgg attaacagtc cgtcgctcta ccgactgagc
    19021 tatcggggaa tggggcgtat tatagcgtcc ggaaaaaatg tgtcaatcct taattttgga
    19081 aaaatgggcg acaaaacgac aagcatatga atcagaaaga cattaagacc gatgccttaa
    19141 aaggattgcc gttgtatgaa tttccacagc cgtcatcaca ccatatttaa gcccgatgag
    19201 ccgttctgcc ctcccccgct taaaacaatg ccgtctgaac ttcgccgcgt tccaaagcca
    19261 gtaaaaactg tttgcggttc aaaccgcctg cgtagccggt cagtttgccg tcgctgccaa
    19321 tgacgcggtg gcagggaatc aggacagaca ccttgttctg cccgttggcg gcggcaacgg
    19381 cgcggacggc tttggggttg cccaaatgct gcgcctgttc cttgtagctg cgcgtttcac
    19441 cgtaaggaat cgccaaaagc gcgtcccacg cctgtttttg aaacgccgta ccaatcattt
    19501 ccaaaggcgt ggcaaaggtt ttcagacggc attggaaata caaatccaat tcctgccgca
    19561 aaagttgcgt ccgctcatcc tcccgaaaca caaaccgtcc gcgcaaggct ttttggacgg
    19621 cggcaatttc ctgttccaaa tgcttctgcc cgacaaattc cagcaaacac aagcctttac
    19681 cgccgaacac cgccagcatc tcgcccaaag gcgtggcaat ggcggcacac accagctcgt
    19741 tcaagctgtc gggataacgc acttccaaca gacggatggc tcggcggata cggacatatt
    19801 cttcaggcgc gcagccgata ttgtcccaaa aatcccgatc gaactgcccg gcttcgcatt
    19861 ccgtcagatt gggatgcggc ataacgccgc actcaaaaac ccgggtttcg aggcaatggc
    19921 ggatttcatc ccattttgac ggcagattgt ttaaggaagg cagggtaatc attcgtttgc
    19981 tccgtatctc tatcatggat ttggcggcaa aatccccaat tctcgtcatt cccgcacgcc
    20041 gatgcaggaa cgggctatga cgtaaatctt gagggttggg ttgcggcaat gcctaaatat
    20101 gcgatatttc taaagcctca gggaaaggaa ttaaagactt cagacgacct gcaaagggtc
    20161 gtctgaaacc acgatttttg catttacgca ttccggcaca tcatccaacc gtttcggcac
    20221 attcctgccg ccgttgacag cctataatga atccacttat tcatcaagca aaggaatcat
    20281 ctatgcaaac ccgcatcctc tccgccgtac tgctggcttt ttcaaccgct gcctttgccg
    20341 ggggcgcatt cacgctgcaa ttcgacaacc cgtccgaaga cggcggcttc acgcaaaacc
    20401 agcttttgag tgcgccttac ggcttcggct gttcaggcga caatgctccg cccgcgctgt
    20461 cgtggaaaaa tccgcccgcc gggacaaaaa gtttcgtcct gaccgtttac gataaagacg
    20521 cgccgaccgg actgggctgg atgcaccggg tggtcgccga cattcccgcc gatgtccgcc
    20581 gccgcaatgc ggcctcgctg caattaagcc gctgcgccag catcgccgac gacccgtccg
    20641 cagccatatc ggcggtaatc agtttgcgga tttgccgcat caggttgacg ccttcgtaca
    20701 cggcaaaacc gatgccgtca tgctgcaacc acgccaacac gccgcaaagc gcggcctccg
    20761 ccgcattgtg cggcacttct tcatccgtca gcaccgccgc cgcataatca aacgccgcgc
    20821 ccatccgccc agggtgcggc agcttcaccg catcgcacac tgcctgcgcc gtcccgtatt
    20881 gtgcggcaaa cctttctacg gtttcctgtt cgaaagcaat ccattgcgcc tgatagaggc
    20941 cgtctgaatc gggaatatta ataacgtcaa acgtctgtcc gcctgccaac gcaaccgcct
    21001 cacccgccgc aatcctcaat tcgcgcctcg cccaccgcag cttcttgctt ccgcgccgca
    21061 cgataagcac agctggttca tataccgcca cgctgcggta caaggcggta tgatgttgca
    21121 cgatgccgcc caaagcaccc aatcgttcgc gcgtatgaaa gtaatttgac ggcactatta
    21181 tctccaacac gcagggccgc acataaggcg cagccctgtt taccctgatt tggaaggggt
    21241 tacttcccaa aaataaacca aaatcccact gccccgaatg gccgagtttc aaccatattc
    21301 cgtcggtgtg cctgcaactt gactccctcc cctgtggggg agggttgggg agagggtaaa
    21361 acggggcaga tacagacaat atttccgtta ccgccccgat gccctctccc taaccctctc
    21421 ccacgggaga gggaatggat tgccgttgaa ataaatcgct ctacataaaa aatcaatgtg
    21481 ttatctcaaa cccacgttag gtctaagcaa atggtcggac atccgtatcc ggcaagcaag
    21541 ctgctttcag acggcatttc cagccaacaa gcgcgccaat atcccctcat acaccgcaga
    21601 cagcttcgga atatcgtcca gccgcacgtt ttcgttgatt tggtggatgg tggcattgga
    21661 cgggcctaat tcgatgagtt cttttgcaat ggctttgatg aagcgtccgt ccgaagtgcc
    21721 gccggtggtg gacaattcgg cctcaatgcc gcaggtttcg gcaatggctg cgcgtgccac
    21781 gtcggtcagt ttgcccgctt gggtcagaaa gggctgcccc gaacacgacc actgcaaatc
    21841 gtattgcacg ccgtgtttgt ccaaaatggc gtggacgcgt tgtttcagcc ctgcttcggt
    21901 ggactcggtg gagaagcgga aattgaattt gacgttcaac tcgcccggaa tgacgttggt
    21961 cgcgcctgtg ccgccgttga tattggaaat ttgaaagctg gttggcggga aatattcgtt
    22021 gccttcgtcc cagacttcct gcgtcagctc taacaaggcc ggggcaaaag tatgcacggg
    22081 attgattgcc aaatgcggat aggcaatatg gccttgcttg cctttgacgg tcaggttgcc
    22141 cgacagcgag ccgcgccggc cgtttttaat catatcgccc aatttgtcca cggctgtcgg
    22201 ttcgccgacg atgcagtagt caatcaactc gccgcgcgct ttcaacacat cgacgacttt
    22261 ggtcgtgccg tccagcgcgt cgccctcttc gtcggaagta atcaggagcg cgatgctgcc
    22321 ttgatgatcg gggtgttcgg caacaaagcg ttcgcaggcg gtaacgaaac aggcgatgct
    22381 ggttttcatg tccgccgcac cgcgcccgta taatcttccg tcgcgctcgg ccggctcgaa
    22441 cgggggcgaa tcccattttt cgacagggcc tgtcggtaca acgtcggtat gccctgcaaa
    22501 acagacgacg ggagctttcg tgccgcgtcg caaccagatg tttttggtgt cgccgaaatg
    22561 gagttcttca gccgcaaaac cgattttgtg caggcgttcg gcaaggagtt tttggcaatt
    22621 ttggtcgtcg ggggtaacgg atgggcggga aatcaattct ttggcgagtt ctagggattg
    22681 ggtttcggtc atatttgttc acttttgaaa ttagaccatc tgaaacgttc tgaatgtgat
    22741 tttcagacgg catttaggtt aggttggcat acggggtggg tatttttacc cgtcagtctt
    22801 ctgaatcatt tgccgtgaca cgcttcgtaa agcggcagca aatcttccac cgtttccgct
    22861 atccatttcg cgacatcctg cctgcccaaa tcgtcgcgtt cgatgtgttt gccgatgcag
    22921 aagaaatctt cgtcgttttg caaccttcgg tcggattcat tttgctgcgc gacagtgcgg
    22981 tagtcgtcgt attcgctttc cgcgccgtgc cacatatcga aagaagcgta tttttcagta
    23041 tcaaaattat ccaaccaacg gttgtaatca ggcagcgcaa tcggcgacac gtcggctttg
    23101 tagcaatgcc agtccaagct gacactcaaa cggcggcggt tgagcagtat cgacaaaatc
    23161 gcggcggaat ttttatattg ttcgtatttg aagtaggcaa agaaatgggc gcgaacctgc
    23221 cagccgttgc accagcgttc gatgtgcggc ggcgcaaacg gcgcacccaa ttcggcggca
    23281 acctgctgaa tcagctgctg ccatatctgc cagttttcct tgtagtcggc tttgatttgc
    23341 ggaatgcttt caggctggta ttttttaagc tgggcaaact ggaaaaacgg gatattgaac
    23401 aaatcgcaac ttttcggggt cagcataata tatccttgag acgattgttt cagacggcat
    23461 tatttgcgcc ggcgcgccgc cataatttcg ccgatttcgg tcagtttttc ttttgggata
    23521 aaggtgttgc ccatatcaaa cagcggctct tcaatcgcca aatgaacatc atatcccgcc
    23581 acaaaacgtt tgaacgcttc ctcatcgggg acataagcgt tgtctgcttc gagtttggca
    23641 aattcggcgg aaacagccgc ccagttgtcg tgcagcccga tatgttggcg caaaagctcg
    23701 tccacgcttt cttgggcttg cggcgcatat tgcagcagca gcgggaagaa gttttcttct
    23761 tcgtcttcat ggtgcagcgg cgcggcaacg ttgaaatact gggcgatttg gcggatggtt
    23821 tgcaaaacaa tctgattgca gccgttttcg gcgatatagt ccgacagcat ggcgacttgt
    23881 ccgcaaaaac ggcgcacttt gccgtggcag gcatacagca tttcaatcgg ttcggcaaag
    23941 gtaacgcttt tggtttcaaa cggattcatg ttttcgttct caacgggcac ttttcaagca
    24001 gtcattttat aataaaacag cctgcacaaa gcaggctgtc cgtcttttga gactttaagc
    24061 ggattaatcg accaaagtca ctttgccgtt catcaaagca ccgtgacctg ggaaggtaca
    24121 agcgaatttg tattcgccgt cagccagttt ggcaggatcc aaagtcaggg aagactcttc
    24181 gccgccgccg atcagtttgg tgtgggcaac aacgcgcgca tcgtcaggtt tgacatagtc
    24241 ggtatcggca gcacctacgc cgtctttaaa tacgccgtcc atgtcttcag ctttggcaat
    24301 cacaaggttg tgacccatgc tggctttggg ttgcgtaccg gtatgtttca gagtgatggt
    24361 gaactcttta catgctttgc tgacttggat gtctttggtg ttgaactgca tattgtcgtt
    24421 ggattcgaca gttgccgcac agttgccggc agcaggggct tcggcagcat ctgcaggagc
    24481 agcttcggcg gcaggtgctt cggaagcggg tgcttcagca gcatgagttg cctcggcagc
    24541 aggcgcggca ggttcttgag agcaggcagc caaaccgata acggcagcag aaatcagagc
    24601 cagatacgtt ttcataacaa atctccaatc gataaaataa tattcggttt tacggaaatc
    24661 aaagtgcaac cgccattaac aaaaccttga aaaagattcc gccgcgttgc acaaacagat
    24721 gtttcggggc ggcattttgc tacaaatttc atttgaaatc aaagcctgtc tgcaagttta
    24781 caatcgttta ctttaaaagg ggcaatttta ccccgaacct atttctttag tattagaccc
    24841 attatccttt acttcttaat attaacggat gtttacacaa attcccgtat acattttatg
    24901 cgccatgcct tctaaccaag tttgccaatg cctccgccaa ttcgggatgc cgtttttcca
    24961 actttaccgc cgccgaaccg aaactctcca gcgcagcctt actcaaatgc agggtattgg
    25021 ttttcggcgg tttttccggt ttcggggcca gcctgaccga aacagagcgt atcgaagcat
    25081 caagccctgc caactgcggc aataccgacg gtgcaatcat tttcaagcgc gatgccgcca
    25141 tattgtttgc cgccaaaagg acaagcctgc cgtcttcgat acatgccgtc tgaaaatgcg
    25201 ggtgcaggtt ggcaggcagc agttttttca cggcggcatc caaccgccgc cactgtcccg
    25261 cctgtttcaa aagtccggaa agcagcgcgt cccgcctgcc caactgttcc aaattcataa
    25321 aacatacacc caaaaagatt gaaataccgc aaacgcgcct ttatttcaga cggcattagc
    25381 actttgcaca aacgcttgtg ttaaaatcgc gttttcgccc actattatat caggcgcagg
    25441 aattattcat gctgacaaac attgccaaga aaatcttcgg cagccgcaac gaccgcttgc
    25501 tgaaacaata ccgtaaatcc gttgccagaa tcaacgcgct cgaagaacag atgcaagccc
    25561 taagcgatgc tgatctgcaa gccaaaactg ccgaattcaa acaacgcctc gccgacggtc
    25621 agactttgga cggcattttg cccgaagcct tcgccgtctg ccgcgaagcg tcccgccgca
    25681 ccctcggtat gcgccacttc gacgtgcagc ttatcggcgg tatggtgctg catgacggca
    25741 aaatcgccga aatgcgtacc ggcgaaggca aaaccctggt cgccaccctc gccgtctatc
    25801 tcaacgcgct ggccggcaaa ggcgtacacg tcgttaccgt caacgactac ctcgcctcac
    25861 gcgatgcggg catcatggaa ccgctctata atttcctcgg ccttaccgtg ggcgtgatta
    25921 tttcagatat gcagccgttc gaccgtcaaa acgcctatgc cgccgatatc acctacggca
    25981 ccaataatga attcggcttc gactacctgc gcgacaatat ggttaccgac caatacgaca
    26041 aagtgcagcg cgaattgaat tttgccgttg tcgatgaagt ggattccatc ttgattgacg
    26101 aagcgcgcac tccgctgatt atctccggcc aggcagacga caacatccag ttgtaccaaa
    26161 tcatgaacac cgtcccgccc cacctcgtcc gccaagagac agaagaaggc gaaggcgact
    26221 actgggtaga tgaaaaggcg caccaggtca tcctgagcga agccggccac gaacacgccg
    26281 agcagattct gacccaaatg ggattgctgg cagaaaacga ctcgctctat tctgccgcca
    26341 acatcgccct gatgcaccac cttatggcgg cattgcgcgc gcatacgtta ttccacaaag
    26401 accaacatta cgttatccaa gacggcgaaa tcgtcatcgt tgacgaattt acaggccgtc
    26461 tgatgtccgg ccgccgctgg tccgaaggcc tgcatcaagc cgtggaagcc aaagaaggcg
    26521 tggaaatcaa acgcgaaaac caaaccctcg cctccatcac cttccaaaac tatttccgcc
    26581 tgtacaccaa gctctccggc atgaccggca cagccgatac cgaagccttc gagttccaaa
    26641 gcatctacaa cctcgaaacc gtcatcatcc cgaccaaccg ccccatacaa cgcaaagact
    26701 tcaacgacca gattttccgt tccgccgaag aaaaattcga agccgtcgtc aaagacatcg
    26761 aagaatgcca caaacgtggg cagcccgtcc tcgtcggcac aaccagcatc gaaaactccg
    26821 aactggtctc ccatcttttg caaaaagccg gtctaccgca caacgtcctc aacgccaaag
    26881 aacacgaacg cgaagccctg attgtcgccc aagctggcaa agtcggcgcg attaccgttg
    26941 ccaccaatat ggcgggacgc ggtacggaca tcgttttagg cggcaacctg aagcaccaaa
    27001 ccgatgccat ccgtgccgac gaaaccttga gcgacgaaga gaaacaggca caaatcgccg
    27061 cactcgaaaa cggctggcag gcagaacacg ataaagtaat ggaagcaggc ggcctgcaca
    27121 tcatcggtac ggaacgccac gaaagccgcc gtatcgacaa ccaattgcgc ggacgttccg
    27181 gccgtcaggg cgaccccgga tccagccgtt tctacctttc gtttgaagac ccgctgctgc
    27241 gcttattcgc actcgaccgc gccgccgcca tcctcaaccg tctcgccccc gaacgcggcg
    27301 ttgccatcga acacaacctg ctgacgcgcc aaatcgaagg ggcgcaacgc aaagtcgaag
    27361 gcagaaactt cgatatgcgc aaacaggttt tggaatacga cgacgttgcc aacgaacagc
    27421 gcaaagtcat ttacagccag cgcaacgaaa ttctgaccag caaagacatc agcgacctga
    27481 tgcaggaaat ccgttctgat gtcgtcagcg acctcgtgga tacctatatg ccgcccgaca
    27541 gcatggaaga acaatgggac atcccgactt tggagaaccg tctggctgcc gaattcagac
    27601 tgcacgaaga catccaatcc tggctgaagg cggacaatgc gattgacggt caagacatca
    27661 aagaacgcct gatcgaacgc atcgaaaacg aatatgccgc caaaaccgaa ctggtcggca
    27721 agcaggcaat ggccgatttc gagcgcaacg tgatgttgca ggtcatcgac aaccaatggc
    27781 gcgaacacct cgccgctatg gactacctgc gacaaggcat acacctgcgc agctatgccc
    27841 aaaaaaatcc gaagcaggaa tataaacgtg aagcctttac catgttccaa gacctgtgga
    27901 acggcatcaa attccatatt gcctccctgc ttacctcggt tcaaatcgaa caaaaccctg
    27961 tcgcggtggt tgaagagcaa cccatcggca acatccagtc catccattcc gaatcgcccg
    28021 atatggaaga acttttgggt cagtcgcaaa ccgatctggt taccgaagcc tttaatcccg
    28081 atgggacgga tttcagcccc gaagccttgg aagcgcgggg acaaatcgtc caccgcaacg
    28141 acccctgccc ctgcggcagc ggtttgaaat acaaacaatg ccacggcaaa ctggcttgag
    28201 cgtttgaacg caaatgccgt ctgaacatcc ggtttccggc ttcagacggc attttgcctg
    28261 aaccgccaca tccgaccgcc attccgaaaa atcccgattt cgtaccgtcc aaactaaaaa
    28321 caggcatccg ccccgccgaa ctatgattcc atccgacttc attgacgaac ttctggccaa
    28381 aaccgatatt gtcgacatca tcgacgaaca ggttccgctg aaaaaaggcg gggcgaacta
    28441 tatggcgtgt tgcccgttcc acaaggaaaa aacgccgtcg ttttcggtca gtccgaccaa
    28501 gcagttttac cactgtttca gttgcggggc gcacggttcg gcgattggtt ttgtgatgga
    28561 acatcagggg ctgtcgtttc cggaggcagt gcagttcctt gccgaccgcg tgggtatgat
    28621 tgtgcctaaa gtgcgcgggc agaacgataa tcccgaagtc cgtgccgaac gtaagaaaaa
    28681 gcagcagacg ctggaagaaa cgacagcggc ggcggctgat ttttatgcgc aacagttgaa
    28741 attcaatccg acggcgaagg cttatttgga caagcgcggc ttgagcgcgg aagtcatcgc
    28801 gcattatggt ttgggctacg cgcccgacgg ctggcagcct ttggcgcaag tattccaacc
    28861 gtacccgaat accgcgttgg tggatacggg tatggtgatt gacaatgagg ggcggtatta
    28921 cgaccgattc cgtcatcgga ttatgttccc catccgcaat ccgcgcgggc aggtcatcgg
    28981 tttcggcggc agggtactgg acgactccaa acccaaatat ctaaactctc ccgatacgcc
    29041 tttgtttgat aaggggaaaa acctttacgg cttgtatgag gggcgtgccg ctgtcaagga
    29101 agcggagcgg attctggtgg tcgaaggcta tatggacgtg gtcgcgctgg cacagttcgg
    29161 cgtgggctac ggtgtggcgg ccttgggcac ggcaacaaca gctgaacacg tcaaaatcct
    29221 aatgcgtcag gcggacagta tttatttctg tttcgacggc gacagcgcgg ggcgcaaagc
    29281 ggcttggcgc gcgctggaaa acgcgctgcc gcagttgaag gacgacaaat cgctgcattt
    29341 tttgttcctg ccggaagaac acgaccccga cagttacatc cgtgcctacg gcaaagcgca
    29401 atttgaagac gcgcttctga atcaaagcaa gcccttgtcg gagtatttct gggaacacct
    29461 ttcagacggc attcatctca atacgcagga aggcaaggcg gaattggtaa aaaccagttc
    29521 gccgcttttg gcgcagatta ccgcgccggc attggcttat ttgttaaaac aacggcttag
    29581 cgaactggtc ggcatcgacc ccgacaacct cgcgcaactg ctgggacagg aagcgccgaa
    29641 gcggcacgtc aaacaaaaaa actacaaact gccgccgatt tccgtcaaac agcccgttat
    29701 gccgacattg gtgcaacggc aaatccgcag cctcttgata aatccggatt gggctgcata
    29761 tatagacctg cccgattatc tggcgttgga cggcgatttc gcctgccttg ccaacctcgc
    29821 cgaaaccatt aaaaaccatc cttccgtgcc ggcaaccgca caggttttgg aacatatgcg
    29881 cggttcgccc tacgaagaaa caatcaaccg catcttccgg tcggcccttc aatcggaaga
    29941 aatggaaggc ggcggcgaag aagattgcga aaacttccaa atcggcatca aaaaactgct
    30001 caatgagtta aaatacagcc aaatcgaaac attaaaacaa aaaagcctgc aatccggctt
    30061 aaatgaaagc gagaaaaaac ttttgctgtc gctgctgacc gcaaaacaaa attgaccggc
    30121 ggattccgcc atccgtaaac cgttatgccg tctgaaaagc attcaccccg gctgcaacaa
    30181 cgacacctgc agaacaccca tccccaaaag ccttcagacg gcatcagagt accctactct
    30241 gccacgcctt caggtgcgtc caaacgcaaa ccgtcggcat cttgccaaca gaaagcagac
    30301 aatgtccaaa aaccaaaatc acgaagaata ccaagacgat gcccgccccc tgacgattga
    30361 ggaacaacgc gcgcgcctgc gccaactcat cattatgggc aaagaacgcg gctacatcac
    30421 ctactccgaa atcaatgacg cactgcccga cgatatgtcc gatgccgatc aaatcgacaa
    30481 catcgtcagt atgatttccg gtttgggcat ccaagttacc gaacacgccc ccgatgcgga
    30541 agacatattg ttaagcgaca atgccgccgt taccgacgat gatgccgtcg aagaagccga
    30601 ggccgccctt tccagcgcag attccgagtt cggcaggacg accgaccccg tccgtatgta
    30661 tatgcgcgaa atggggcagg tcgacctgct gacccgcgaa gacgaaatca tcatcgccaa
    30721 aaaaatcgaa aacgccctga aaaatatggt tcaggccatc tccgcctgcc cgggttccat
    30781 tgcggaaatc ttagaactca tcgaaaaaat ccgcaaagac gaaatccgcg tcgacgaagt
    30841 cgtagaagcc attatcgacc cgaatgaagt attgctcaac gaattgggct tggggcactt
    30901 ggaaaccaca gcacccgaga aaccttccaa cgacaattcg gacgaaaacg aagacgacga
    30961 agaatcggaa gaagatgcgg atgaaatctc ggcagccaat ctcgccgaat tgaaacaaaa
    31021 agtcatcggc cactttgccc aaatcgaaaa agactacaaa aaaatgattg gccgtttgga
    31081 aaaacaccac agccggcaca aagactatct cgcctaccgc gacgcgattg ccaacaaact
    31141 gctggaagtc cgtttcgcca cccggcaaat cgacagcctc agcagcagcc tgcgcggaaa
    31201 agtagaaaac atccgcaaac tcgaacgcga aatccgcgac atctgcctcg accgcgtcca
    31261 tatggagcgc gactacttca tccaaaactt cctgcccgaa atcaccaatc tggaatggat
    31321 tgaagaagaa atcgccaaag gcagggtttg gagcgacgcg ctcgaccgct tccgccacgc
    31381 catcctcgaa aaacaaaccg agttggcgga tatggaaaaa gaaacccgca tttccatcga
    31441 agagttgaaa gaaatcaaca aaaatatggt gtcgagcgaa aaagaaaccg cagccgccaa
    31501 acaggaaatg attcaggcaa acttgcgcct cgtgatttcc atcgccaaaa aatacaccaa
    31561 ccggggctta caattccttg atctgattca ggaaggcaac atcggtttga tgaaagcggt
    31621 cgataagttc gaataccgca gaggctataa attctccacc tacgcaacct ggtggatccg
    31681 ccaggcaatt acacgctcga ttgccgatca ggcgcgtacc atccgcattc cggtacatat
    31741 gattgaaacc atcaacaaga tgaaccgcat ctcgcgccaa caccttcaag aaaccggcga
    31801 agaacccgat tccgccaaac ttgccgaact gatgcagatg cccgaagaca aaatccgcaa
    31861 aatcatgaaa atcgccaaag agccgatttc gatggaaacc cccatcggcg acgacgacga
    31921 ttcgcacttg ggcgacttca tcgaagatgc caacaatgtt gcgccggccg atgcggcaat
    31981 gtacaccagc ctgcacgaag taaccaaaga aatcctcgaa agcctgaccc cgcgtgaggc
    32041 aaaagtcctg cgtatgcgtt tcggcatcga tatgaacacc gaccacacgc tggaagaagt
    32101 cggcagacag tttgacgtaa cgcgcgaacg catccgacaa atcgaggcaa aagcactccg
    32161 caagctgcgg catccgacaa gaagcgaccg tttgagaagt ttcttggaca gcgaagacag
    32221 caagctgtaa accaaaaaac cgcaggtttc aaatacctgc ggttttttct tacacaataa
    32281 acaacgcttc cacatatccc acactcctat cccgagacct ttgcaaaatt ccccaaaatc
    32341 ccctaaattc ccaccaagcc atttagggga ttttccatga gcaccttctt ccaacaaacc
    32401 gcccaagcca tgattgccaa acacatcgac cgtttcccgc tattgaagtt ggaccaagtg
    32461 attgattggc agccgatcga acaatacctg aaccgtcaaa gaatccgtta cgtccgagac
    32521 caccgcggtc gtcccgccta tcccctgttg tccatgttca aagccgtcct gctcggacaa
    32581 tggcacagtc tctccgatcc cgaactcgaa cacagcctca tcacccgcat cgatttcaac
    32641 ctgttttgcc gttttgacga actgagcatc cctgattaca gcaccttatg ccgctaccgc
    32701 aattggctgg cgcaagacga caccctgtcc gaattgctca aactgatcaa ccgacaactg
    32761 accgaaaaag gcctaaaagt agagaaagca tccgccgccg tcgttgacgc caccattatt
    32821 cagaccgccg gcagcaaaca gcgtcaggcc atagaagtcg atgaagaagg acaaatcagc
    32881 ggccaaacca caccgagtaa ggacaaagat gcccgttgga tctctacaaa ctcggttaca
    32941 aacaacatac ccgtaccgat gcggaaggct atatcgagaa actgcacatt acccccgcca
    33001 atgccaatga gtgcaaacac ctgtcgccgt tgttggaagg actgcccgaa ggtacgaccg
    33061 tctatgccga taaaggctac gacggtgcgg aaaaccggca acatctggaa gaacatcagt
    33121 tgctggacgg cattatgcgc aaagcctgcc gcaaccgtcc gctgtcggaa gcgcaaacca
    33181 aacgcaaccg gtatttgtcg aagacccgtt atgtggtcga acaaagcttc ggtacgctgc
    33241 accgtaaatt ccgctatgcg cgggcagcct atttcgggct gattaaagtg agtgcgcaaa
    33301 gccacctgaa ggcgatgtgt ttgaacctgt tgaaagcggc caacaggtta agtgcgcccg
    33361 ctgccgccta aaaggcagcc cggatgcctg attatcgggt atccgggcag gattaagggg
    33421 atatttgggt agaattaggc ggtatttggg gcgaaaatag acgaaaacct gtgtttgggt
    33481 ttcggctgtc gggagggaaa ggaattttgc aaaggtctca tcctgttatt tt
//
LOCUS       DAXIHS010000013        32778 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXIHS010000013 DAXIHS010000000
VERSION     DAXIHS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.013"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(151..453)
                     /locus_tag="ACFOF2_000522"
     CDS             complement(151..453)
                     /locus_tag="ACFOF2_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC7106894.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(425..712)
                     /locus_tag="ACFOF2_000523"
     CDS             complement(425..712)
                     /locus_tag="ACFOF2_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC7106895.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            792..1397
                     /locus_tag="ACFOF2_000524"
     CDS             792..1397
                     /locus_tag="ACFOF2_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106896.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            1376..1672
                     /locus_tag="ACFOF2_000525"
     CDS             1376..1672
                     /locus_tag="ACFOF2_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC7106897.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            1669..1878
                     /locus_tag="ACFOF2_000526"
     CDS             1669..1878
                     /locus_tag="ACFOF2_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106898.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(2048..3230)
                     /locus_tag="ACFOF2_000527"
                     /pseudo
     CDS             complement(2048..3230)
                     /locus_tag="ACFOF2_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(3672..4319)
                     /locus_tag="ACFOF2_000528"
     CDS             complement(3672..4319)
                     /locus_tag="ACFOF2_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC7106899.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(4316..4684)
                     /locus_tag="ACFOF2_000529"
     CDS             complement(4316..4684)
                     /locus_tag="ACFOF2_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC7106900.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(4694..5365)
                     /locus_tag="ACFOF2_000530"
     CDS             complement(4694..5365)
                     /locus_tag="ACFOF2_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC7106901.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGIFGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(5530..6249)
                     /locus_tag="ACFOF2_000531"
     CDS             complement(5530..6249)
                     /locus_tag="ACFOF2_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC7106902.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(6333..7646)
                     /gene="hpnE"
                     /locus_tag="ACFOF2_000532"
     CDS             complement(6333..7646)
                     /gene="hpnE"
                     /locus_tag="ACFOF2_000532"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC7106903.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARYADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKGKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(7676..7882)
                     /locus_tag="ACFOF2_000533"
                     /pseudo
     CDS             complement(7676..7882)
                     /locus_tag="ACFOF2_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224552.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8044..8916)
                     /gene="hpnD"
                     /locus_tag="ACFOF2_000534"
     CDS             complement(8044..8916)
                     /gene="hpnD"
                     /locus_tag="ACFOF2_000534"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC7106904.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYCE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            9087..10949
                     /gene="hscA"
                     /locus_tag="ACFOF2_000535"
     CDS             9087..10949
                     /gene="hscA"
                     /locus_tag="ACFOF2_000535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC7106905.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGGNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMPIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLEAEELQQI
                     RQGIADLQGRVKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            11025..11672
                     /locus_tag="ACFOF2_000536"
     CDS             11025..11672
                     /locus_tag="ACFOF2_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC7106906.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRTELFAVDENLVKRLFLSLRGEIVFPKNETA
                     ESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRL
                     FKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR"
     gene            11708..12469
                     /locus_tag="ACFOF2_000537"
     CDS             11708..12469
                     /locus_tag="ACFOF2_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC7106907.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGASPASTTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            12525..12866
                     /gene="fdx"
                     /locus_tag="ACFOF2_000538"
     CDS             12525..12866
                     /gene="fdx"
                     /locus_tag="ACFOF2_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC7106908.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(13153..13740)
                     /locus_tag="ACFOF2_000539"
     CDS             complement(13153..13740)
                     /locus_tag="ACFOF2_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC7106909.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            13953..14150
                     /gene="iscX"
                     /locus_tag="ACFOF2_000540"
     CDS             13953..14150
                     /gene="iscX"
                     /locus_tag="ACFOF2_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC7106910.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(14218..14619)
                     /locus_tag="ACFOF2_000541"
     CDS             complement(14218..14619)
                     /locus_tag="ACFOF2_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC7106911.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            14830..15789
                     /locus_tag="ACFOF2_000542"
     CDS             14830..15789
                     /locus_tag="ACFOF2_000542"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC7106912.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15857..17167
                     /gene="tilS"
                     /locus_tag="ACFOF2_000543"
     CDS             15857..17167
                     /gene="tilS"
                     /locus_tag="ACFOF2_000543"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC7106913.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            17218..18003
                     /locus_tag="ACFOF2_000544"
     CDS             17218..18003
                     /locus_tag="ACFOF2_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC7106914.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            18013..18210
                     /locus_tag="ACFOF2_000545"
     CDS             18013..18210
                     /locus_tag="ACFOF2_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106915.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            18472..18824
                     /locus_tag="ACFOF2_000546"
                     /pseudo
     CDS             18472..18824
                     /locus_tag="ACFOF2_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18836..19141
                     /locus_tag="ACFOF2_000547"
     CDS             18836..19141
                     /locus_tag="ACFOF2_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106916.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            19203..20579
                     /gene="mpl"
                     /locus_tag="ACFOF2_000548"
     CDS             19203..20579
                     /gene="mpl"
                     /locus_tag="ACFOF2_000548"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC7106917.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            20786..21838
                     /gene="bioB"
                     /locus_tag="ACFOF2_000549"
     CDS             20786..21838
                     /gene="bioB"
                     /locus_tag="ACFOF2_000549"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC7106918.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(21985..22551)
                     /locus_tag="ACFOF2_000550"
     CDS             complement(21985..22551)
                     /locus_tag="ACFOF2_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC7106919.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITNKQGKFIS
                     GTIIPITQADDKIPKIDPEKPLSGGLFI"
     gene            complement(22675..22914)
                     /locus_tag="ACFOF2_000551"
     CDS             complement(22675..22914)
                     /locus_tag="ACFOF2_000551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106920.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            22901..23134
                     /locus_tag="ACFOF2_000552"
     CDS             22901..23134
                     /locus_tag="ACFOF2_000552"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC7106921.1"
                     /translation="MQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(23197..25056)
                     /gene="ilvD"
                     /locus_tag="ACFOF2_000553"
     CDS             complement(23197..25056)
                     /gene="ilvD"
                     /locus_tag="ACFOF2_000553"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC7106922.1"
                     /translation="MPDYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPHSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDIAQIER"
     gene            complement(25228..26997)
                     /gene="cysI"
                     /locus_tag="ACFOF2_000554"
     CDS             complement(25228..26997)
                     /gene="cysI"
                     /locus_tag="ACFOF2_000554"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC7106923.1"
                     /translation="MTVQTKTKGLEWQEKPLSNNERLKTESNFLRGTILDDLKDQLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSYYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDDLDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(27024..28838)
                     /locus_tag="ACFOF2_000555"
     CDS             complement(27024..28838)
                     /locus_tag="ACFOF2_000555"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC7106924.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRLAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQAMPPADLQTVPESRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADCVEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQHKIREQAAELWQWLQEGAYVYVCGDAAKMAKD
                     VEAALLDVIIQAGHLNRDDAEEYLNTLREEKRYQRDVY"
     gene            complement(28835..30121)
                     /locus_tag="ACFOF2_000556"
     CDS             complement(28835..30121)
                     /locus_tag="ACFOF2_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC7106925.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDESTYGTVAAGMIL"
     gene            complement(30160..30372)
                     /locus_tag="ACFOF2_000557"
                     /pseudo
     CDS             complement(30160..30372)
                     /locus_tag="ACFOF2_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
     gene            complement(30406..31146)
                     /locus_tag="ACFOF2_000558"
     CDS             complement(30406..31146)
                     /locus_tag="ACFOF2_000558"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC7106926.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRDIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(31157..32608)
                     /gene="cysG"
                     /locus_tag="ACFOF2_000559"
     CDS             complement(31157..32608)
                     /gene="cysG"
                     /locus_tag="ACFOF2_000559"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC7106927.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
BASE COUNT         7245 a         8604 c         9325 g         7604 t
ORIGIN      
        1 gacggaaagt ggcgggaata acgaaaggcg ggaatgaccg cgcaaaaagc cgctgccccc
       61 ttcggacggc accggcaaca aaaaaccgca cggccgaaac cgcgcgggaa aggatagtcg
      121 ggcgcgcccg ataagcagcg gccgcccccg ttatttcaat tgggcgacat attggcgcaa
      181 aacctcgttg atgcgcgtct gccagccctt gccgccggcg cggaattttt cgaccacatc
      241 ggcggacagg cgtatggtaa cgagttgttt cggggttttg cctgtgtttc gtttttgcat
      301 tacgcccttt tcttccaatt gtttttgatg ggaaaaaagc acctgtgcca agtcttcggg
      361 cagggcttcg gcaatggggc gggcaagcgc aaaatcttcg gcggcaagtt cccgcacttc
      421 gccgtcagcg tttgttaagg attgacgttg catatttttt aacctctctt ttattcgctt
      481 tgcgaaaact gatgacacgg atgccgtctt ttatcggcgt aaaacagaca atgtgcaggc
      541 gttgcgtatc gcctagataa gcagcggcaa cataacgcgg ttcggggtaa tcaaagcgga
      601 catcgggcac aataacggcc gttgtccagc gtatttgccc gactgattca aagggcaaat
      661 tccgctcttc gatattgcgt tgattttttt cggagtcaaa ttcaatcttc attgcagctt
      721 gcagcgtatt ttgtcgttac attataagcg gcaaaaaacc aaaatgtaaa tacaaaaaag
      781 gaaaccccga aatgaccatc tatttcaaaa acggctttta cgacgacaca ttgggcagca
      841 tccccgaagg cgcggttgcc gtccgcgccg aagaatacgc cgcccttttg gcaggacaga
      901 cgcagggcgg gcagattgcc gcagattccg acggccgccc cgttttaacc ccgccgcgcc
      961 cgtccgaata ccacgaatgg gacggcaaga aatgggaaat cggcgaagcc gctgccgccg
     1021 cccgtttcgc cgaacaaaaa accgccacgg cattccgcct cgcggcaaag gcggacgaac
     1081 tcaaaaacag cctcttggcg ggctatcccc aagtggaaat cgacagcttt tacaggcagg
     1141 aaaaagaagc cctcgcgcgg caggcggaca acaacgcccc gaccccgatg ctggcgcaaa
     1201 tcgccgccgc aaggggcgtg gaattggacg ttttgattga aaaagttgtc gaaaaatccg
     1261 cccgcctggc cgttgccgcc ggcgcgatta tcggaaagcg gcagcagctc gaagacaaat
     1321 tgaacaccat cgaaaccgcg ccaggattgg acgcgctgga aaaggaaatc gaagaatgga
     1381 cgctaaacat cggctgaaaa aatacgttta ccacctgttg gtagccatcg accaactgtt
     1441 caacgcccta accggcggcg cggcggacga aaccctctca agccgcacct atcgcggcgc
     1501 gcggctcgcc caaaagccca aaacccgctg gaaggtttta tacaccctga tcaacggcgt
     1561 atttttcgac cgccaccact gccgacaggc gtatatcagc gaactgaaag gcaggcagca
     1621 cgacgcgcgg ttcaaccaaa gccgcgccgc tggggaaaag gggacgcgat gaactacttc
     1681 ggcatggtag agtttctgcg cctgatggca atggtgcggc cgccatttgt cttcttctcc
     1741 ggcacccgca gcgaactgcc tgcttatctt gacctcgtgg cagaactgcg cctgaccgga
     1801 tgggagcgtt ttgcaggcag ccaaaccttg actgtgagca gcacatcaac agcaattcaa
     1861 gctacgacga ccacctgatt tataagttct gaccgcaagt agcgtactac ttttaaaggc
     1921 ataagataat ccccgtttaa cagaccatta aacggggata aatttgtgca aaagctaata
     1981 caatttccta cgcttcggcg gtgcaaaagc tgccgccaat tcgtgcaaaa gctgccgccg
     2041 ccttacattc cagtgcaatg ccgtctgaaa cttcgctaat ctcgggttgc tgcgtgctgt
     2101 gtttttcttc cgtactcagc aaaaagccgt acaaacgttc caatcgggcg cggtaggcag
     2161 tgaatttgtt atagaacatc cggaagaaag acagcgcgtt ttgcagtcgt gcgaaggctt
     2221 ggacggtctg ctggatgtcg ccgattttga tttggccggc aaacaggcgt ggggcttgca
     2281 aaataatggg aaaaagcttg atgccgttgg tgaacatatc gttaaagcca ctcaggcaga
     2341 cgctttgccg cgcaatgcgc cagcggttgc ggataatggc tttgaagcgg tcggcaagcc
     2401 ggtcgtgttc gtgtttttcg ccgctgtaaa acgccacgct ttcggcgtgg tcgcgcacgc
     2461 ggatgaggga ataacggtag tcgccgttga gcttttcgtt ttcatagttg taacgaatca
     2521 atgggttgcc tatccacatg gcgataaagg tcgccaaaat tacaaagata aagacgaacc
     2581 aaacaatgcc gcgaggaatg tcaaagccaa acacggtcag gatgcccgcc aagccccaca
     2641 aaacaacggc aaactccagc gaggtaacga ccgaattgac catgccgcgc acaaattcga
     2701 tggtcgaggc gatgaaatcc tgtgcgtcct gttggatacg ttggtcgatg ttgtccggcg
     2761 cgtggcggcg catttgcagg cggtagtagt ttttgtcggc aagccagcgt gctgtcagca
     2821 cttcgttgag ccgctccgac catttaatcg ccaagccttg attgaggaag tcgttgacga
     2881 cgttgttaaa cgcccgtatc agcaccacgc ccgcgttcat tgcggcaaac atccaaaatg
     2941 ccgaagcatt tcaaatcctg catcgagtcg taaagccctt tggacataaa ggtactcaac
     3001 acattcagcc gcatttcggt taacaccagc gtaatcatct ccgtaatcag cagcaagact
     3061 ttgaccgcgc ttttcggtgt cagacaaagg cgcaggatat aggcaaactc gcgcccgaaa
     3121 cgcgttttcc gtgtcaaaaa cagaatcacc gccgaagcgg cggaaactat caagagagtc
     3181 tgtaaaagcc aaagcggtgt ggaataaagc tcggtttgcc atttctgcat gggaaatttc
     3241 ttacggtatc aatgccgtct gaaaaagacg ggtacagttg attttttgat gaagtttggg
     3301 gaagttttgc cggtcagggt acattgcgtg ttaatttata gtggattaac aaaaaccagt
     3361 acagcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt
     3421 gaatcggttc cgtactattt gtactgtctg cagcttcgtc gccttgtcct gatttttgtt
     3481 aatccactat accatacaac cacgccggaa ttaagtttaa atttgaataa aaggttcggg
     3541 ttctgcaaaa tacagaaccc gaaccttgtt cggatattga aaccggctgc ccgattttgg
     3601 gcggtgcggc ttgcaagtat caagattcgc atatgccgtc tgaagctcgg agaggttcag
     3661 acggcatatg cttatttggg ctgctcttcg acgaatctcg gacctttcaa gatgccgttg
     3721 tgagaatagg gcgacagcag gttgtatgcg gcggttttgg aaacctgata accgcggtcg
     3781 gtcaggctgt tggcaatctg attgaccact gcgctgacca aagcccccaa caggccgctg
     3841 ttgctgttgt tgctgccttc gcggatgctg gccgaacccg accacaactc ttttccgttg
     3901 cgggaatcga ccagccgtgc tttggcggat acggtcgtca cgctgtctaa aatttgatat
     3961 gaagtgccgt attcggtaac cgtaatgtac aaaaccgcat cattgccgaa aatctgatgc
     4021 agtttttccg gccggacggc gtgaatatcg gcggcattgg tcaagccgtt ttgtttgaag
     4081 gtttcctcca cgactgcggc ggggaagacg taatagccgg cttcggaaag cggagcggcg
     4141 gtcgaagcca gtacacccca tgttccgttg acatcgggcg attcgttcag cgggggaacc
     4201 accaaaattg aagccggttt gctttccttg aatgacgtgt agtcgaagtc gggagctttt
     4261 tgaacttggc aggcagacag cgccaacacg gcggcaagcc ctaaaatcaa aggtttcatc
     4321 gcttgcctcc tttaccggtt ttcatcagga agtccataaa tacgcccgat tcgggaaaca
     4381 gccttttctc ttcttcaaac tggcggaacg cgccctcttt gtctcccgaa cgggaaagca
     4441 gcagtcccag atgggcgtgc gcacccgggg cggcattcat ttttttgttg ccggcttcca
     4501 caaagtattt ttccatcttt tcggtctgct tgcccaacga agtgtcgtcg tttttcaaac
     4561 cttcatagac ggtatcggga tagccgccgt aataatacag ggatttttgc ccgttgccgc
     4621 cgcaggcggt cagagccaag accgccgcac acagcgacaa acggctcaag gttttcggat
     4681 tcatcatttc tccttaacgg ttgggttgcc atgcgccgtt gtcaacagcc tgaaccaggc
     4741 tgttgacggc ttcgcggatt gccaagtcta aaactttgcc gttcaaagtc gcatcgtagc
     4801 cggaagtgcc gccgaaaccg atgatttcac ggttggaaag tgcgtattcg cccgcgccct
     4861 gtgcggaata gacgatttcg gaagtattga cgttgacgat attcagagcc acttttgcat
     4921 aggcgatttg cgatttgccg cgaccaaaaa tgccgaagag ctgatgatcg ccgacatctc
     4981 tgcgtccgaa ttcggttaca tcgccggtaa cgacataatc tgcgcctttc aggttatgcg
     5041 ctttgccgga aatgccggat tcctgtttta atgcgttcaa attggtgcgg ttcagtacgt
     5101 tgaagcggtt ggtctgttgc aggtgcgtta ccagaatggt ttttgcctgg ctgcccaaac
     5161 ggtcttcccc gtcggagaaa atgccttttt ggaagctgga gcggttgtcg aatgttccga
     5221 cggaaatcgg ggtacgaaca ccgtgatatt gcgtattgta ggaggcgact tttgctacct
     5281 cgagactgcg tgaggattcg gtcgcacagc cggtcagtga aacggcagcg gcggcaagga
     5341 caacggcggt ggaaacggtt ttcataaaat ttaccctaag gtcaagttaa ggaaataacg
     5401 ggttgtcatt attgtcctta tgtaaattta agtcaaggtg tttgtctgtg cgggacggat
     5461 gcgcgcggaa ggtacggata tttttcaaac ggaatcgcgg cgcgggcggg atgcctgcct
     5521 tttccggatt tagaggtaaa cgatttcgcc gctccgccct ttgctttcgg tacttgccca
     5581 ccagacaaat gcgggcagca cgtccccgta gcttttgcgt tcgcttttgg cttcgcccgg
     5641 atgggatttg atgcgttgcg gggaattgat ggggccgggg acgaggacgt tggcgcgcag
     5701 gttgccgaag cgttcccatt cgtcggcggc gactttgcac aggtagttca acgcggcttt
     5761 ggacgcgccg aagccgcccc agtaggcttt gggtgtttcg ccgtggcttt cgccgacgaa
     5821 gatgacggac gcgtcgggcg actgcttcag cagcgggaac agggcgcggg tcagccccat
     5881 aggtgcgacg gtgttgatgc ggtattggtt gacccattcg gcgacggttt ggaaatccag
     5941 cggcgagagg gcgtaaaaat agccggcgca gtggacgatg ccgtccagtt tgccttgcgt
     6001 ggcttcggca atggtggcgg cgaactgatt gaattctttt tcttccgcac tcatcaggtc
     6061 gaaacggatg gcgaagggtt cggggtgtcc ggcttcgaca atcgcgtcat agactttttc
     6121 cagttttttc tgatggcgtg ccaccagaat cacggttgcg cctgccgccg cataggcttt
     6181 ggcgacctgt tcgcccagac cttgcgatgc gccggtaatt aagatggttt tgtcggacag
     6241 tgtcgccata ctttttcctt tttggtggtc ggtcgggggt attttagcgt tttgccgcac
     6301 cttgtaaagc gtcccgatac cggccggcgt tttcagacgg catcgctcag gctttgcagg
     6361 caggcttccg ccgacgcgaa acctgattgt acggcggctt cgagcgtggc ggggtagtct
     6421 gggtggaggt agtcgccggc ggggaagatg cggtgccggt gcaaccacga caaatccggc
     6481 ggcggggcat cggctgcggt tgtggcgcgt ttttcggtga tgacgcgcac ggcttcgggt
     6541 tcgcccaaat gcggaaggat gcgtttgagg tcggcgtgaa ctttgtccgc ccacgctcgg
     6601 tttgtaaacg cgccgacgcg gtcggaaacg ctgatgacgg cggacacttc gttttcaggc
     6661 agtccgagcc tgccccggca aagcaaccat tgtgccgtgc cgtcggcaag gccggtcagc
     6721 ggggcgggca ggcggacggg ttcggcgtag cgcagataga cggtggtgat ggcgtgatag
     6781 cgaaggtttt gatatgccgt ctgaacgtgt tcgggcgtgc cttcgggcag gagcgcggcg
     6841 gcgtggtagg gcgcggtggc ggggacggcg gcatcgaaag cttcgccgtt gacgagcact
     6901 ttcccgtccg ggagggggtt cagacggcat acgcgcgttt cgaggcggat gtccgcgccg
     6961 agccgttgaa gctccgccaa ggcaggttcg gcgacgattg cgcccaaatc ctgcttgggt
     7021 aggagatagt cgctgccgga ttttttcgtc agcacgccgt cggacaaaac gttgcacaac
     7081 acgcgcaggc ttgcggtttc caaaggcgtg ttgagcgcac cccacaccag cggctgccaa
     7141 aactgcatca cggcggcacg cggcacgttc cgctgtttca gccattgtgc aactgtcgtg
     7201 tcgggctgtc cgaggcgtgc ggacttctgc aaatcggaca tatcggcaag cagtttgcct
     7261 ttgaatgcgg acggtacgcg ccgggcaagc agcacgccgc ccaaaatatg cagcggtgcg
     7321 ggcaggggga gggcgcggaa ctgtaaaccg ccgtgcatat gccagtgcag cggtacgcgc
     7381 aaaaaggcgg cacgggggtc tgaaccgatg gttttcatca ggcgcaacac gccccggtat
     7441 gcgccgagca aaatgtgctg cccgttgtcc aaaaaaccga aaccgtcggt atttccggcc
     7501 agtgcgcgcg ccctgccgcc cgcctgccgg ccggcttcaa acagggtaac gtcggcgtac
     7561 cgcgccaagg tgacggcggc ggacagtcct gcccagcctg cgccgatgac ggcgattttc
     7621 gggcgcggat gcggcgtgtt catcatttat tcctccaatg gttttgcagc cgtatctatt
     7681 tccgtttctg aaaatgacgg tagaaaagga tacaggctaa aaataaaggc agcagaaagc
     7741 gcgcataggg atatcacgga tggtctgcat gccagtatcc gtaccgtccc tgtggaacgg
     7801 tatcatagcg gtaattgtcg gggaaaaagt tgatccaaac cgttactgcc ggccataagg
     7861 ctatatccct gactgctttc atttgcttct gctcctgttt aaattcccgg caattcgatt
     7921 tcaaagcgcg aacgccaacg ggattgcgcg gttacgatgc agactttcag acgatggttg
     7981 aaaccccgtc cggcggggct gtgggaatcg ggtttgcctg acgggcggcg ggcggtatgc
     8041 gccttatgcc cgttccggcg tgccggggcg cggtttgaat ccgaataacc aggttttcag
     8101 ggcaatgcgt tttttgcgcg gcgaagggag ggcgattttg tatttgagga cgttttgtgc
     8161 gccgtcccgg tcgatttcgt tcaatagctc gtaataaacc gccgccataa ccagtccgac
     8221 tttttgggct tttttatcgg cagccggcag cagcgatacg gcttcgcagt aggtttcacg
     8281 ggcgcgtttg atttggaacg ccatcaattc ggcaaaattg cccgtcgggc tgcattgcaa
     8341 aatcacgctt gcgggtacgt caaaccgccg catttcctcc atcggcaggt aaatccgtcc
     8401 cctgcgcgcg tcttcgccga catcgcggat gatgttggtc agttgcagcg caagtcccat
     8461 cttgtcggcg tattccagcg tttggtcgtc tgaaaacccc aaaatccgcg caatcaggca
     8521 gccgaccacg cctgcgacgc ggtggcaata cagtttcaat tcttcaaaac tgccgtaacg
     8581 ggcttgaacc aaatccatct gcatcccgtc gattaaggct tccagttcat atttcggcag
     8641 cttgaaggtt tccttaactt gccgcaaggc ctgattgacg gggtgttccg gcatcgcgcc
     8701 gccgaatacc ttgtccaaat cgccgcgcca ccagttcaat gtcgcctgtg caacatcggg
     8761 gttggaacat tcgtcaacca catcgtccaa ttcgcggcaa aaagcatata aaaccgttac
     8821 cgcatcccgt ttttcctgag tcaggaaacg gaagcccgac aaaaaactgg agcggctttc
     8881 ttctgctttt tggcggcaat agtcgagtcc tttcacgatt tatattccta atgatgggcg
     8941 ggaaaggcgg attttatcgg catttggcgg tagagggcaa tttcggcggc acgacctaat
     9001 ccttagcggt ttgctcaact atcggcgcaa attctgttaa aatgccgctt tccttccttt
     9061 acacaccgca ccgacaggca gaatttatgg ctcttttgca gatttcagaa ccgggtatgt
     9121 ccgccgcccc gcaccggcac cgtttggcgg caggcatcga tttgggtacg accaacagct
     9181 tggtcgccac cgtccgcagc ggcagtgccg cctgcctgcc cgatgccgaa gggcgcgtta
     9241 ccctgccttc cgtcgtccgc tatctggaaa acggcggcat tgaagtcggc aaaaccgccc
     9301 tgtccgccca aaaaaccgac ccgctgaaca ccgtcagctc cgccaaacgc cttatcgggc
     9361 ggacgcttgc cgatctgcat caaaatacgc actacctgcc ttaccgtttc ggcggcaatc
     9421 aacgcgttat cgaactgcat acgcggcagg gggtgaaaac gcctgtcgaa gtgtcggcgg
     9481 aaatcctcaa aacccttaaa tcgcgcgccg aagaaacctt gggcggcgat ttggtcggcg
     9541 tggtgattac cgtccccgcc tatttcgacg atgcccaacg ccaggccacc aaagatgccg
     9601 cgcgtctggc gggtttgaac gtattgcgcc tgctcaacga acccaccgcc gccgcaatcg
     9661 cctacgggct ggacaacgcc tcggaaggca cgtttgtcgt gtacgactta gggggcggca
     9721 cattcgacgt atccgtattg caactgacca aaggactgtt tgaagtcaaa gccaccggcg
     9781 gcaacagcgc gttgggcggc gacgatttcg accaccgcct gttctgccgc ctgctcgaac
     9841 aaaacggact ctcccaactc aacgaacaag acagccaact cctgctctcg ctcgtccgcg
     9901 ccgccaaaga acaattaacc acgcaaaccg aagcgcgcat tcaggcgacg ctttcagacg
     9961 gcatgccaat cgacacaagc atcagtcgcg ccgagttcca caacctgacg cagcatttgg
    10021 tgatgaaaac gctcgaaccg gtcacacagg cgttgaaaga tgccggtgtc ggtaaaaacg
    10081 aagtcaaagg cgtgattatg gtcggcggtt cgacccgtat gctgcacgtc caacaggcag
    10141 tcgccacctt cttcggacaa accccgctga acaacctcaa tcccgacgaa gtcgtcgcgc
    10201 tcggcgccgc catacaggca aacgtcctcg caggcaacaa aaccgacggc gaatggctgc
    10261 tgctggacgt tacacctttg tcgctcggtt tggaaaccta cggcggcttg gcggaaaaaa
    10321 tcatcccgcg caattccacc atccccaccg cgcgcgcgca ggactttact accttcaaag
    10381 acggtcagac cgcgatgacg atacacgtcg tacaaggcga gcgcgagctc gtttccgact
    10441 gccgcagcct tgccaaattc accctgcgcg gcattccgcc tatggcggcg ggtgcggcgc
    10501 gtatccgcgt aaccttccaa atcgatgcgg acggtctgct gtccgtttcc gcccaagaac
    10561 aaagcaccgg cgtacaggcg caaatcgaag tcaaaccttc ctacggcttg gacgacgaca
    10621 ccatcaccca aatgctcaaa gacagcatga gcaacgccgc cgaagacatg gcggcacgtg
    10681 cccgagccga agccgtggtc gaagccgaaa gcctgaccga tgccgtcaac gccgccctcg
    10741 agttggacag cgatttgctg gaagccgaag aattgcaaca gattcggcaa ggcatcgccg
    10801 atttgcaagg ccgtgtgaaa gacggaaaag ccgaagacat ccgtgccgcc gccgccaaac
    10861 tcggcagtat taccgacaat ttcgccgcca aacgcatgaa ccgcaacatc caacgcgcgt
    10921 tgactggtca aagcgttgat aatatttgac ggcaaatggt ttcagacggc atagatataa
    10981 cctgataccg tctgaaggct cgaaaacact tgaaaaacat cgatatggaa aagtcaggca
    11041 ttgtctatct gatgaaaacc gtcatcaagg gcgtgtataa aatcggcatt tcggacgtaa
    11101 gcaattttga aggcagaatg cgccatttgg aaaacaacgg ctatgcgaac gttgccggat
    11161 tggaacgcat cctcgccgtc aaaaccgaca attacaaaga aaaagaaaac ctgctccatg
    11221 aaatttttag taaaagcagg accgaattgt tcgccgtgga cgagaatctt gtaaaacgtt
    11281 tgtttttatc gcttcgcggc gaaatcgtgt tcccgaaaaa cgaaacggcg gaatcggaat
    11341 ttgaaaaaag cgtccacgaa cgcaggcagg aagggaatgc cggatccggt cgcaaacaac
    11401 tgcttgattt ggtacggcgc ggacaccggg aataccctta cgcgctgccc cggcttttgg
    11461 cgggcgcggc atcctacaag ccgaaaaaat cgaaaatccg cctttttaaa gaagcatatt
    11521 tacgcaaaag cggtacgagg ctgaccgacg aaattgcaga cggcatccat atttacacct
    11581 gtttttcgcg ggcggatttg gaaaaagcct attccgaata tttggaactt ttcaaatccg
    11641 aatcggatgc cgaaggcaga aaaccacggt aaggtgcaaa cagataccgt acacgttgag
    11701 gagcagtatg atgggcgatt ccgtcattta ttatgtagaa caggcagacg aaccggtaaa
    11761 ccgtgccggc gaacgcgccc gtaaaacatt caaatacttt tggcgcgagc ttttttggga
    11821 acgccgccgc atcattcccg ccttagattt tgccatggtc aaagcccctt ttttccaaga
    11881 cggcgaagac ggcgaaattt gcgaacatat gtggatcgac gatatttatt ttgacggtct
    11941 ttacatttat ggcgtgctga acaatgaacc cggcgaactg accaatgtcg aacaaggcga
    12001 aagcatttgc gttccggttg acgacatcag cgactggatg ttcgtgtgca acggcattcc
    12061 ctacggcggc tttaccgtac aggcaatgcg cgggcagatg acggaagagg agcgcaccga
    12121 acacgatgcc gcatggggaa tcgatttcgg cgatcccggg cagatattgc tggtgtatga
    12181 agaaaaagaa catcccgaaa atctggaaga gcatccgatg tgccggaact gtattgacga
    12241 ttttcggcaa cagttgtccc aaaattcgga ttttctgcat gagcaggacg aagacggcta
    12301 tacgccgctt catcatgaag ccatcgcagg aaatgcactt atggttcaag ccatgcttga
    12361 atatggcgca agtcctgcct caacgacatc ggaaggctat accgccctcg attttgcccg
    12421 cctggcgggc tggcaaaatg ttgccgacct gctcgaaccg agacattagg cagacagttt
    12481 tccgaaaaac ggacgcaacc actttttaca gaaagacaat aaaaatgccc aaaattaccg
    12541 tacttccaca cgcgacgtta tgccccgagg gtgcagtcat cgataacgcg ccggaaggca
    12601 aaaccgtcct cgacgtactg ctcgaccacg acatcgaagt cgatcacgcc tgcgaaaaat
    12661 cctgcgcctg caccacctgc cacgtcatca tccgcaaagg tttcgacagt ttggaagaac
    12721 cgaccgaatt ggaagaagac ctgctcgacc aagcatgggg tttggaagcc gattcgcgcc
    12781 tgagttgtca ggcggttgtc gccggcgagg atttgattgt ggaaatcccc aaatacacca
    12841 tcaaccacgc gcgcgaagaa cactgaaaac aggccgtctg aagccggcac gcttcagacg
    12901 gcattgttgc gcggataagg cgcaatcgcc cgaaaacagg cgttcgtaca ggcggaactt
    12961 tcgattctat agcgaattaa caaaaatcag gacaaggcgg cgagccgcag acagtacaaa
    13021 tagtacggca aggcgaggca acgctgtacc ggtttaaatt taattcacta tatattgatt
    13081 tttatcggtt ttctgacgga acggattggt gcggcatccg aggcggatta ctcgggcgcg
    13141 atgcacgggt atttatcggt tttgcagccg gaaaaaccgc cggcgggtta ctttcagggc
    13201 ggcggtcggg cggtatgccg gatagcgttc cactttcgct tctatattga ttttgattgg
    13261 ttttctgacg gaatgacccg atgcggcatc cgggacggct tgtgtttttt cctgcccgcc
    13321 tgccggattt tcccatcctt gcgtgaaacc gaaagagacg gcggcgcggc ggacaagctc
    13381 gagatagcgt ccttcaagct ccgggcaggc ggcggacacg ctttctgccg taaccgtgaa
    13441 gccgccgccc gacccggaaa ggcgttccgc aatctgcgtg tagatacgcc aacgttgggc
    13501 gcgcaacatt gccgcatcgc aaccggcagc cgctttatcc aaggcaatca ggccgcgccg
    13561 gttttggtgg tgaaggcaaa ccgttactct ggagaggata tgttgcccgt ccaatatttg
    13621 atacagtttg cgtatcagca gaatcaggcg gtcaaactcc tccatctccg acagggaatc
    13681 aggatggtcg gaagcggtat aaaccagttt ggacaatttt gcggcaaaca tattgttcat
    13741 caatcttcct tgtcggttga aaccccgctc ttcggggcgg tagaatcaga tttgtttggg
    13801 agggacaact cctcccagat caggacgaca cataggctgg tgcttgatgt gttgtccggc
    13861 gagttgaaac attcagccat cctcaaggtg cggcagtttt gccgaaacat attctacacg
    13921 gcttcaatgc cggacgataa aaggaaattc atatgaaatg gaccgacacc cggcgtatcg
    13981 ccgaagaact ctatgacctg cacggcgaaa ccatcgatcc cagaaccgtg cgctttaccc
    14041 aactgcgcga cctgattatg gcattgcccg aatttgatga cgatcccgcc cgttgcggcg
    14101 aacgtattct cgaagccgtg cagcaggcat ggatagacga ggcagaataa gtttcgggaa
    14161 tgccgtctga aatacggcgg tacgcggttc gtgcttctgt ttgcagtggg aacggtttta
    14221 ccagtctcct tttttcagcc tatccagttg gcggcggtcg cgcttggtcg gtctgccgtc
    14281 gggatgggcg gaagtgatgc ggctgaattg gtcgagctgt ttgcgctctt ccctcaatgc
    14341 tgccgttttt gcgtcttctt catacagaag ccgcgcctcg gatgccgggc ggcgttggtg
    14401 gttcaaacct ttaaccttga ttttataggg aagggaattg agcgtcaggt cgataatatc
    14461 gccgatgtct atggttttac tgtttttgac cttcgagccg tttacttgaa ccctacccag
    14521 ttcgatgtgc ttttgcgcaa gggaacgggt cttgaaaaaa cgtgccgccc aaagccattt
    14581 gtccagccgc atggcggaag aatcgtgctt gtctttcata cgattttgtt tgaaataatt
    14641 gaatttgttt cgagtttagc ataagatacg ccgcctataa ctagtatata tgcactaatc
    14701 cactgttttc catgctgtcc gaacacaaaa agagggtgtg gaaaagccgt tttggacaat
    14761 aaattaactg cggaatatgc acaaatagcg tatgatagcg gcagaatctg ttgatgagag
    14821 cttcattcta tgaaacctgt ttttttggat tttgaacaac ccatagccga actgaccaac
    14881 aaaatcgatg agctgcgttt cgtccaagac gagtctgccg tcgatatttc ggacgaaata
    14941 caccgtttgc agaaaaaaag caacgacctg accaaatcga ttttcagcaa actcacaccc
    15001 gcccaaattt cacaggtttc ccggcatccg cagcgtccct atactttgga ttacattgag
    15061 gcactgttta ccgattttga agaactgcac ggcgaccgcc actttgccga cgattatgcg
    15121 attgtcggcg gattggcgcg tttcaacgga caaagcgtga tggtcgtcgg gcatcagaaa
    15181 gggcgcgaca ccaaagaaaa aatccgccgc aacttcggta tgccccgtcc tgaaggctac
    15241 cgcaaagccc tgcgcctgat gaagacggca gaaaaattcg gcttgcccgt catgaccttt
    15301 atcgatacgc cgggcgcgta tcccggcatc ggcgcggaag aacgcggaca gtcggaagcc
    15361 atcggcaaaa acctgtacga actgacgcgc ctgcacgtcc ccgttttgtg taccgtcatc
    15421 ggcgaagggg gctcgggcgg tgcgttggcg gtcgccgtag gcgattacgt caatatgctg
    15481 caatattcga cctattctgt tatctccccc gaaggctgcg cttcgatttt gtggaaaacc
    15541 gccgaaaagg cggcggatgc ggctcaggct ttgggcatta ctgctgaccg cctgcaaaag
    15601 ctggacttgg tcgataccgt catcaaagaa ccattgggcg gcgcgcatcg ggatttcggg
    15661 caaaccatga aaaacgtaaa agccgttttg gaaaaacaac tgcacgaagc gcaaagcatc
    15721 ccgcttgccg atttgctttc gcgccgtttc gaccgcatta tggcttacgg caaattttcg
    15781 gaacaataat tcaggtagaa caagcagcaa gcagtttgtc tgaaactgct tgctttttct
    15841 ttatcgggac ggaaccgtgc tgactttaga tgcgtttgag caatgcttga aggattgttt
    15901 tcctcaaggt ctgaatggaa aaaaaacagc ggtggcatta agcggcggct tggattccgt
    15961 cgttttgctg catctgcttg tccgcgccgg gaaaaaaggc ggttttattc cggatgcatt
    16021 gcatatccat cacggcttga gtccccgtgc cgacgattgg gcggatttct gccaaaacta
    16081 ttgcgatatg ctcggggtgg ggctggaaac ggttaagatc tgcgtggaaa aaaacggttt
    16141 gggcatcgag gcggcggcgc ggcaaaagcg ttatgccgcg tttgccgaaa aaggctttga
    16201 cgttttggcg ttggcgcacc acagggacga tcaaatcgaa acctttatgc tggcggtcgc
    16261 gcgcggcggc ggtttgcgcg ctttggcggc tatgcccgcc gtccgccctt ttggggaaaa
    16321 aggcattatc tggcggccct tgctgccttt ttcgcgccaa gatatatggg attatgccca
    16381 aaaacacggt ttgccgaata tcgaggacga aagcaatagc gatacggctt atttgcgaaa
    16441 ccgcttccgg caccgtattt tgcccgaact ttcggcgcag attccccatt tcgggcggca
    16501 tctgttgaac aatgtccgtg ccttgcagga ggatttggct ttgttggacg aggtcgtcgt
    16561 tcaagactgc cgttgggttt gcggagccgg ttatttcgat acggcgcggt ggctgacgtt
    16621 ttccccgcgc cggaaaaccc atattttgcg gcattttttg aaggaaaacg gcattcccgt
    16681 gccgaatcag aatgcccttg ccgacattgc ccgggttttg acggaggcaa aaaccggacg
    16741 ctggaacgtg caaggctttg aattgcatca ttatgcaggc aggctgtttg tgttccgacc
    16801 ggaaaaaacg gataaactgc ggtttttgaa agacaggcag ataagcggaa atttaaggga
    16861 aatattggcg gggcagggat ttgttttaaa acgtcatccg ttcggacttc ccgggcatct
    16921 tttggagcag gacggaatct tgcggacggt tgcggcatcg gacaggctgg ctgtcggcgg
    16981 tatccataag gatgtgaaga aaatccttca ggaaaagcgg gttttgcccg cgctgcgccc
    17041 gttttggccg gtcgtcgccg acaaggacaa ccgtccgctg gcgttggcaa attacggtac
    17101 ggattttcaa atctcggttt cagacggcat tttgcccgtc catccggatt ttcccgtttt
    17161 attttgatgg aatcggaatc gccgttacct attatatatt cttgaaaagg cggtaaagtg
    17221 aagcatatca gttcggcaaa caatgaacac atcagacacc tgcaccgcct gttgtctcaa
    17281 gggaagttca gacggcaata cgcccaaacc gttttggagg gcgtgcacct gcttcaggtt
    17341 ttcctgcaat ccggcgggat gccggtcggg gtatatattc ccgaagcgaa aatgccgtct
    17401 gaagaagtcc gtaaattgac ggcggttttg ccggaagacg gggttttttc cgtttcagac
    17461 ggcatattga agaaaatcag cagcctgact tgcgcggatg atgtgcttgc gctgattgat
    17521 attccggatg cgggtgcttt gccggccggc ggcgactgtg tggttttgga cggcgtgcaa
    17581 gatccgggca atgtcggcac ggtgttgcgg agcgcggcgg cggcaggaat cggcgcggtc
    17641 gttttgggca aaggttgtgc ggacgcgtgg tcgcccaaag tgctgcgagc cggaatgggc
    17701 gcgcatttct tgtcggagat ttatccgcag gcggatttgg aaatatggtt ggcgcgttat
    17761 gaagaccgtg tgtttgccac cgcgttgcgt gaggaaaaac aggcggttct gtataacgaa
    17821 gatttgcgcg aaccgacagc ctgggtgttt ggcaacgaag gcgcgggtgt cggcaaagcg
    17881 gttttagaca gggcggacaa gtgcgtcagg ataccgatgc acgatgcaac cgagtcttta
    17941 aatgtcgcga tggcggcgac aatctgcctg tttgaacaga tgcggcagcg gtcggcgtat
    18001 tgaggaaaag aaatgccgtc tgaaaaaatc tattacggcg tattgatttt cttatgtatc
    18061 gcttctatgc tgctgtcgcc gtttttttat gcgggtgctt tgaagcccaa gaaggcggca
    18121 ttgcggaagg acgggcagtg gaaactcatc ttattgtcca ataccgtggc ggcggcggtt
    18181 ttggcttggg tgtggtggaa atggttttga cagatattgc tcaaaatcgt gctaatggaa
    18241 tccgaacaaa taaagaattt tgtaaaaaat ttgttaaatc aacgaattaa agttttgtgg
    18301 aaaacaaaac agctctaagc aaatagggcg tttgtcggta aatacggaag agttgcggca
    18361 ttatcgggca tctttaacaa gtagtgccgt cttggcaggc aatcggtttt tatgggcagc
    18421 ttgcaaaatc gcggatataa aattgcgaat cggttaaagt gtggggacgc tatgaaaaat
    18481 tgcgaatttt ttatgacccg acaagggcaa tctatgatag cggtgcagat tatttaacca
    18541 gagaaaaaca tagattcgtc gtaattgcaa atagtgcttg ggggctattg cttaatttat
    18601 cttgttatta tgacaaggtt ttggaaaagc ggaaaatacc gttcggcaaa caggaaattg
    18661 atgacgatat ggacaaagtg tccgcccata agcggaagtt taaagatatt tctgaaatca
    18721 aagtagggga tggttgggaa tacccgttca attatgagca gggaatgaaa gaattagatg
    18781 aagtgctatt gaaatacatt cccttttttg aagaagaacg ataaaggagg ttgatatgcg
    18841 cgtatctaaa ataattggaa gtatgttgct tgttacagcg gttcagaccg tattttcggc
    18901 aaatgtttac gcgtgccgcc ataatggtaa aaccagttac agccaaactc cgggaaaaca
    18961 ttgtaccaac gcgggtttgg ggcgggatcg ggtgtacagt tcggttagac cggcagtaaa
    19021 agacagggcg gaagacgcag gggtcggcga ttattcggac acggtgaggg acgaacacgt
    19081 ccaaaatccg agagagaata cgcataagga tgcttcgaat accggtgcaa agacgcattg
    19141 attgaaaccg aatcagccat gacggcgttg aataatagaa tttgaacgat tagggaactc
    19201 tgatgaaaca catccacatt atcggtatcg gcggcacgtt tatgggtggg attgccgcca
    19261 ttgccaaaga agcagggttt gaagtcagcg gttgcgatgc gaagatgtat ccgccgatga
    19321 gcacccagct cgaagccttg ggcataggcg tacacgaagg cttcgacgcg gcgcagttgg
    19381 aggaatttaa agccgacgtt tacgtcatcg gcaatgtcgc caagcgcggg atggatgtgg
    19441 ttgaggcgat tttgaaccgc gggctgcctt atatttccgg tccgcaatgg ctggctgaaa
    19501 acgtgctgca ccatcattgg gtactcggtg tggcggggac gcacggcaaa acgaccaccg
    19561 cgtccatgct cgcatgggtc ttggaatatg ccggactcgc accgggcttc cttatcggcg
    19621 gcgtaccgga aaacttcagc gtttccgccc gcctgccgca aacgccgcgc caagacccga
    19681 acagccaatc gccgtttttc gtcattgaag ccgacgaata cgacaccgcg tttttcgaca
    19741 aacgctccaa attcgtgcat taccgtccgc gtaccgccgt gttgaacaat ctggaattcg
    19801 accacgccga catcttcgcc gatttgggcg cgatacagac ccagttccac cacctcgtgc
    19861 gtaccgtgcc gtctgaaggc ctcatcgtct gcaacggacg gcagcaaagc ctgcaagaca
    19921 ctttggacaa aggctgctgg acgccggtgg aaaaattcgg cacggaacac ggctggcagg
    19981 ccggcgaagc caatgccgat ggctcgttcg acgtgttgct tgacggcaaa aaagccggac
    20041 acgtcgcttg gagtttgatg ggcggacaca accgcatgaa cgcgctcgcc gtcatcgccg
    20101 ccgcgcgtca tgccggagtc gacattcaga cggcctgcga agccttgagc acgtttaaaa
    20161 acgtcaaacg ccgcatggaa atcaaaggca cggtaaacgg catcactgtt tacgacgact
    20221 tcgcccacca tccgaccgcc atcgaaacca ccatagaagg tctgcgtcag cgcgtcggaa
    20281 acgcccgcat cctcgccgtc ctcgagccgc gttccaacac gatgaagctg ggcacgatga
    20341 agtccgccct gcccgaaagc ctcaaagaag ccgaccaagt gttctgctac gccggcggtg
    20401 tggactggaa cgtcgccgaa gccctcgcac ctttgggcgg caggctgcac gtcggcaagg
    20461 atttcgatgc cttcgttgcc gaaattgtga aaaacgcccg aaccggcgac catattttgg
    20521 tgatgagcaa cggcggtttc ggcggaatac acaccaaact gctggacgct ttgagatagc
    20581 ccgggcgatg ccgtctgaaa gcccttcaga cggcatcgtc cggctgcgcg gcacaaaggc
    20641 ggaaaaaccg tttgccccgt attttcaaac gcgttacact tgccgccgct gttttcagcc
    20701 atttgattac ccgcaaccgc cgtcattgcg ccggcggttt gcctgtcagc gtcattgcgc
    20761 cgctgtaaat acgaaagaac acattatgac cgtatccccc gtcgccttgc gccgtaagac
    20821 cgagtgcaag cctcatccca ccgcgcgcta ttggaaaaaa tgcgatgtcg aagccctgtt
    20881 cggacttccc ttcctcgacc tcatttacca agccgccgaa atccaccgcc aaaatttcaa
    20941 cccgcgcgaa atccagcttt ccacgctgtt gtccatcaaa accggcggct gtcccgaaga
    21001 ttgcgcctac tgtccgcaat cggcgcacca caacaccaat ctgggcaaag aacagatgat
    21061 ggatgtggat gaaatcgtcg aaaaagccaa aatcgccaaa tcgcgcggcg caagccggtt
    21121 ttgcatgggc gcggcgtggc gcggccccaa acccaaagac gtggagacgg tttccgcaat
    21181 catcaaagcc gtcaagggct tgggtatgga aacctgcggc acgttcggta tgctcgaaga
    21241 aggtatggcg gaagacttga aagaggcggg cttggattat tacaaccaca acctcgacac
    21301 cgaccccgac cgctacaacg acatcatcca tacacgccgg catgaagacc gtatggacac
    21361 cttgggcaaa gtccgcaacg ccggtttgaa agtctgctgc ggcggcatcg tcgggatgaa
    21421 cgaaacccgc gccgaacgcg cagggctgat tgccagcctc gccaacctcg acccgcagcc
    21481 cgaaagcgtg ccgattaacc ggttggtcaa agtggaaggt acgccgcttg ccgatgccga
    21541 ggatttggac tggacggaat ttgtccgcac catcgccgtg gcgcggatta cgatgccgca
    21601 aagttatgtc cgactgtcgg cagggcgcag caatatgccg gaagcgatgc aggcgatgtg
    21661 ctttatggcg ggcgcgaact cgatttttta cggcgacaag ctgctgacca cgggcaatcc
    21721 ggatgaagac ggcgacagaa tcctgatgga aaagctcaac ctgtatccct tacagtttga
    21781 gctggaaggc gaggtcgccg aagtggaaaa agcctctggg attaaagtgg attattgacg
    21841 attgcaaaaa tgccgtctga aacccgaaaa aaggctttca gacgacattt gttcggacgg
    21901 tatttccaat atctttttac cggcgcgtga tgttgccgtc gggcgagaca tccagcccgt
    21961 tctcgttggg gaagtcgctg cggttcagat aaataatccg cccgataacg gtttttcagg
    22021 gtcgattttg gggattttat cgtcggcttg ggtaataggg atgatagttc cggaaataaa
    22081 tttaccctgt ttgttggtga taattttgaa aatcggcgca atgccgctga tgccggaggt
    22141 gttgattgcg ccgtaggtgg caaagttgcc gccgctgtaa gagatgaagc ggtcgcggta
    22201 aagttcgacg gcgcgtgtaa cgtgcggccc ctgcccgaat acgacatccg cgccggaatc
    22261 gacggcaagc cgtgcaaact cgacgacgtt gcccctgttt tccccataga agatttcggt
    22321 atcgaacggc aggtgttccg cctgtttccc ttccgcgccg ccgtggaaca ttacaatgac
    22381 gatgtcggct ttctgtttgg ttttggtaat ccgttttcta actttggcat aatcgttcag
    22441 tttgacggcg gcaaggttgg gggcgaagga gacgaagccg gatcttacgc cgtttttctt
    22501 caggatggcg gtttcaaacc tgttttcgat gcccgaatat ttgatgttca attcgtcaag
    22561 gttcgccctt tatgccgttt ccgcaccgca aaccgccggg gtcaagccct cggcgcatcc
    22621 tgccggaacg gaatccccgt gctgccgatt gacggttcaa accccgcgcc cgtttcaaat
    22681 accggcgatg tggacggaca ggatgcgcct gacgaaaaga cagccgatac cgtttccatt
    22741 atcggcgtgg gcgacattat gctcggcagc aattatccgg tcgattacct gcccgatacc
    22801 aatattctga aaaacgtcga atctgccttg caagacgcgg acattaccgt cggcaacctc
    22861 gaaggcacgc tgtttgacga aggcggtacg ccgaaaaaat gtgcaacccc ccaaaatatg
    22921 ctatgcattc cgaacgccct ccgcatacgg gcaatacctt gccgacgcgg gattcgacta
    22981 cctcagcttc gccaacaacc acagcaacga cttcggcgcg caaggcatca cggcaacggc
    23041 ggcgagcggc agctctttta catactcgat cgcgctaaag ccgctgccga taacgaggcc
    23101 aaaattgcgg aaaataccgc catcgcccag ataaatttgt ccatcatcag acctttactg
    23161 ttcagacgag acagcatttg ccgcacgttt tggggcttat ctttcgattt gcgctatgtc
    23221 gcgcaccgcg cctttgtcgg cggaagtcgc catcgcgccg taagctctta atgcggcgga
    23281 gacgtagcgg tcgcggtttt taggcttcca tgctttgctg ccgcgcgctt ccatttcggc
    23341 acggcgtgcg gcaagctctt catcggaaat ggcaaggtgg atgctgcggt tggggatgtc
    23401 gatttcgacg gtatcgcctt cgtgtaccaa accgatcgcg ccaccttccg ccgcttcggg
    23461 cgaggcgtgt ccgatggaca aacctgatgt gccgccggag aagcgtccgt cggttaagag
    23521 agcgcaggct ttgccgaggc ctttagattt caggtaggaa gtcggataca gcatttcctg
    23581 catgcccggg ccgcctttcg ggccttcgta gcggatgatg acgatgtcgc cggcgacgat
    23641 ttggttgccc aaaatgcctt ctactgcgtc ttcttggctt tcaaacacgc gggcgcggcc
    23701 ggtgaatttg aggatgctct cgtccacgcc tgcggttttt accacgcagc cgcgctcggc
    23761 gatgttgccg aacaagaccg ccaaaccgcc gtcttgcgag taggcgtgtg ccacgtcgcg
    23821 gatacagcct ttttcgcggt cgaggtcgag ggttttccac atacggtttt gcgagaacgc
    23881 ttgggtggtg cgtacgccgc cgggcgcggc tttgaagcgt tcgatggcgc gggtgttttc
    23941 agggttggtt acgtcccatt tttcaatcgc gtctttcagc gtcggtgcgt ggatagtgtg
    24001 cacgtcggtg tgcagcttgc ccgctttgtc caattctttc aggatggcga aaatgccgcc
    24061 ggcgcggtgc acgtcttcca tatagtagtc gtggttgttg ggtgcggttt tgcagatgca
    24121 gggcacaacg cggcttaagc ggtcaatgtc ggccattttg aaatcgacac cggcttcgtt
    24181 ggcaacggcc aacaggtgca aaatggtatt ggtgctgccg cccatcgcaa tatccatcgt
    24241 catagcgttt tcaaacgctt ttttggtggc aatgctgtgc ggcagcacgg tttcgtcgtt
    24301 ttgttcgtaa taacgtttgg tgatttcgac aatcatacgt cctgcttcga ggaacaattc
    24361 tttacggcct gcgtgggtcg ccaaatacga accgttgccg ggtagggaca gaccgagtgc
    24421 ttcggtcagg cagttcatcg aatttgccgt aaacataccc gaacacgaac cgcaggtcgg
    24481 gcaggcgttt tgttcgactt cctcgacttg ccggttgctg acattgtcgt ccgccgattc
    24541 gatcatcgcg tcaatcaagt ccaaacggcg ttcgggttgg atgtttgcca cgccgataac
    24601 tttgcccgct tccatcgggc caccggaaac gaagatggtc gggatattca ggcgcatggc
    24661 ggcaatcagc attcccgggg tgattttgtc gcagttggaa atgcacacca gcgcgtcggc
    24721 gcagtgggcg ttgaccatgt attcgatgga gtcggcaatc aaatcgcggc tgggcaggga
    24781 atacagcatg ccgctgtgtc ccatagcgat gccgtcgtcg atggcgatgg tgttgaattc
    24841 tttggcgatg gcgccggctt tttcgatttc gcgggcaacc agctggccca tattgtgcag
    24901 gtggacatga cctggcacga attgggtgaa cgagttggca acggcgataa tcggtttgcc
    24961 gaagtcggtt tccatcacgc cggtggcgcg ccacaatgcg cgcgcgcccg ccatattgcg
    25021 accgtgggtg gaggttttgg agcggtagtc cggcatagtg tgtttccttg tctgctatgc
    25081 cgtctgaacg gcaaaaggct ttcagacggc atttttgttg tgttttgaaa tgaaaaaaaa
    25141 ggtattttat accaagtatc ggaattttgc gggattgaaa cggcgtgcgg caaaaaagaa
    25201 aatccccgca ggaatgcggg gacgggttca ggcgcgggca atcgcgacgg ctttggatgc
    25261 gtcccaaaaa tcaacggggg catttaatac gggtttgacg atgcccgtcc gtatggcgaa
    25321 atcgccgaaa ccttcgccga tattgcgttc tgccgcccat ttgccgatca ggtcgtccaa
    25381 ttcggcaagg atttcgggca gggtgatgtt ttctttgtaa aggcggggga tgcgtacgcc
    25441 ttcacggtcg ccgccgatat ggaggttgta gcgtccgacg gctttgccga ccagcccgat
    25501 ttccgccaac attgcccgtc cgcagccgtt ggggcagccg gtaatgcggg taacgatata
    25561 gtcgtccgac gtgccgtgtt tcgccataat cttatccaga tcgtcgatga agtctggcag
    25621 cacgcgttcg gcttccgcca ttgccaaggg gcaggtcgga aaggaaacgc aggacatcgc
    25681 attttcgcgc agcttgctga catcgttgcg gattaatccg tatgttcggg caagttcttc
    25741 gatttttgct ttgtctgctt cggcaacatt tgccacgatg aggttttggt ttgcggtaat
    25801 gcggaaatcg cctttgtgga ttttggcgat ttccaacacg ccggtcagaa gctgtttccc
    25861 gccttcgtca accaaacgcc cgctttcgat gaaaagggtc aaatgccagt tgccgtctat
    25921 gcctttcacc cagccgatgc ggtcgccgcg cccggtaaat ttgaacgggc gtatgggttc
    25981 gaacggcata cccatacggc gttcgacttc cgcgcggaag ttgtccaagc ccatattttg
    26041 aatggtgtag cgggtgcggg cgtttttgcg gtcgctgcgg ttgccgaagt cgcgctgtgt
    26101 ggttaccacc gcttcggcgg ccttcagcgc gtgttccgga ggtacgaaac ccagttccag
    26161 tgaaatgttc ggataggttt tggtgttgcc gtgttccatt gaaagcccac cgcctgccaa
    26221 aacattaaaa ccggcaagct gtccgccgcc gtctgaaatg gcgacaaaat ccaagtcgtt
    26281 gccgtagcag tccacatcat ttaagggcgg gatgacgact gcggttttga atttgcgcgg
    26341 cagataggtt ttgcccaaaa tcggctcgtc ttcttgaagg aagtcgtcgg aactttgaac
    26401 ttttttgccg tccacccaaa catccagata accgcgcgtg cgcggcagca agtgttcgga
    26461 aatctttttc gcgtattcgt aagcctgttg gtggagtgcc gactcgatgg gattggaggt
    26521 gcaaagcacg ttgcggttca tatccgccgc cgtggcgatg gaatccaaac ccagtttgtg
    26581 caagaggcgg tgcatcgtct gcaacttggt tttcggcacg ccgtgaaatt ggaaggtttg
    26641 ccggttggtc agcctgatgg atcggtaata actgttttcg cgggcgaatt tgtccaattc
    26701 tatccattgg gacggcttga tgatcccacc cggcagccgg cagcgcaaaa gcataaattt
    26761 caagggttcg agttttgcct cggcgcgttc ggcgcggata tcgcggtcat cctgctcata
    26821 catgccgtgg aagcggatga gttggaaatt gtcgcccttg aagccgcccg tgagctggtc
    26881 tttcaaatcg tccaaaatcg tgccgcgtaa aaaattgctt tcggttttca ggcgttcgtt
    26941 gttggatagc ggtttttctt gccactccaa accttttgtc ttggtctgta cggtcatttt
    27001 gtgttccttc cgatttcatt taatcaataa acatcacgct gatagcgttt ttcttcacgc
    27061 agcgtattta aatattcttc tgcgtcgtcc cggtttaaat gtcctgcttg aataatcaca
    27121 tccaataagg cggcttcgac atcttttgcc atttttgccg catcgccgca cacataaaca
    27181 tatgcgccct cctgcaacca ttgccaaagc tctgccgcct gttcgcggat tttgtgctgc
    27241 acatagattt tttcttcctg atcgcgggac caggcgaaat cgtatttatg caggaaaccc
    27301 tctttggcaa actgctgcca ttcggtctga tagaggaagt cggcggcaaa atgcggattg
    27361 ccgaaaatca gccagttttt gccttccgca ttttctgcgg cacgttgttg gacgaaagcg
    27421 cggaacggtg cgacgccggt gcccgagccg atcatcacaa tcggcttgcg gctgtcttcg
    27481 ggcagtctga agccgtcgtt gcgctccaca aatacacgca ccgtgccgtc ttcttcgacg
    27541 caatcggcaa gaaaaccgga tgcgccgccc gttctggcgc ggccttcgtg ttcaaaacga
    27601 accacgccga cagttaaatg cacttcatcg cccacttccg cctgtgctga agaaatcgaa
    27661 tacaaacggg gtgcaagcgg acgcagtaaa cggatgaatt gttctgccgt caggcttgcc
    27721 gggaagcggt gcagcacatc gacaataggc gtgttttgca cgaaatcctg caaaacggcg
    27781 ttatcggcaa tgattttatc gagttcttca taatgggcga acgcggcata gcctttgacg
    27841 aaagccggag tgttttgcgt gagttcgaaa tgagatgaaa gtgcgcgcgc aaccggcatc
    27901 atctttccgc ctgcctgtat ttccgttgcc ggatcgatgc cgagcaggtc taggatttcc
    27961 ccgaccagtg ccggattgtt gtcaaaccaa acgccgagcg cgtcgcctgc ctgatagctt
    28021 aaatccgaac cgctcaaatc aagttcgatg tggcgcacgt ctttatcgga ttggcgggcg
    28081 gtgattttct gattggccag cagggcggcg ggaaaggggt ctgccttgca gtacctgctc
    28141 tccggtaccg tctgaaggtc ggcggggggc attgcctgtg gcgcgggtgt tgcccggttt
    28201 tttgcggctt cttcttttaa gagtgcggcg atattgtctg tccaggcgtt tgcagaggcg
    28261 gtaaagtcca aatccgcatc aacgcgttcg agcaggcgtt tcgcccccaa ttcttcaaaa
    28321 cgccggtcga aatctttacc tgcctgacag aaattcggat aggagctgtc gcccaagccc
    28381 agtacggcaa attggagttt gtccaatttc ggggcttttt tgccgttcag cagtttgtgc
    28441 agcacgacgg cttcttccgg cggttcgcct tcgccctggg tggacgtaac cagcagcagg
    28501 cggcgttcgt ctgcgatgtt tttcgccttg tagtctttca gttcggcgag cctgacttgt
    28561 atgccggcgg ctttcaggct gtccgccgct ttgtcggcaa cggatttcgc attgccggtt
    28621 tgcgaggcgg aaaggacggt aacggaaaag ggttctgccg ccggcaatgc tgtctgaagc
    28681 gcgggcagcc ctgcggatgc cccgtttcct gcttttgccc aagcatagcc ggacagccac
    28741 gcccattgtg ccgcgtccag ccccgacagg agctgcgtga tttcgggcgg cagaggcggt
    28801 aatggcggat ttgtgttctg catatcgtgt tcactcataa aatcatacct gccgcaacag
    28861 tgccgtatgt cgattcgtct atcaggataa acgaaccggc ggcggtgttt tccgcataag
    28921 gcgttgccgt aacgggtttt tgaaggttga tgcggacttt ggcgatgtcg ttcatcttca
    28981 aggattccgc gccggcctct tgttccagcg tgcggacatc caaaacgctt tcaatttccc
    29041 cgacttttgc cggcacggtt tgcgtgccgt gcttgagcag gtatttgcgc gcggtgttga
    29101 gcggacgttc gtcaaaccag caaagcgtgg cttccagatg tttttgcggg gcgagcgggg
    29161 aatttttatc gacaaaaagg tcgccgcgcg aaacatcgat gtcgcggtcc agccggaggg
    29221 ttgccgcctc gccggcaaaa gcctgcgcca cttccccttt cggcgtgatg atttcggaca
    29281 cttcggcggt cagcccgttc ggttcgatgc ggacggtttg cccgacggcg accgaaccgc
    29341 gttcgatgcg cccctgatag cctcggaaat catcggcctt gtcggcatct tggcggacga
    29401 ccagttgcac ggggaaataa aaatcgtcgg cggtgcggct gacttcgtcc gcccccggca
    29461 gggtttccaa aatggacaat aagggttcgc ctttatacca aggcatattg ccgccggggt
    29521 aaacaatgtt gtcgcccaag agtgcggaca ccggtacgaa atgcgcgtct ttcaaaccga
    29581 gctgttcggc aagtcggcgg tatgcctcca caatggcgtt gaatttgtct tcgctgtaat
    29641 ccagcaggtc cattttgttg accgccacca caatatgcgg gcagttgagt tggcggagga
    29701 tggcggaatg gcgtttggtc tgcggcagaa gctgtaaggg ctgcgcgccg aaatccagtt
    29761 gggatgcgtc aaccagtacg actgccgccg aagcggtgct tgcgcccgta accatattgc
    29821 gcgtgtattg ttcgtgcccc ggcgtgtcgg cgatgatgaa tttccgtttc gccgtggaaa
    29881 aatagcggta tgccacatcg atcgtaatgc cctgttcgcg ttcggcttcc agtccgtcgg
    29941 tcaggatgga gaagtctatg gtttctttca aacctttgct tttgccggat tccaaggttt
    30001 tgatttggtc ggacagcagg gctttgctgt cgtagagcag ccgtccgatc agggtgcttt
    30061 tgccgtcatc gacgctgccg gcggtaatga agcggagcgg ggcctggtgt tgtgccgtca
    30121 tattttcttc ctcatatctg cttaaagggt ttttgaaatt tagaaatagc cttctttttt
    30181 gcgtttttcc attgccgcct cgcttgcctg atcgtccagc cgggtcgcgc tgcgttcgga
    30241 aatgtcggca accgctgttt ctctgataat ctccgtcggc gtggacgcgg tgctttctac
    30301 cgggcaggtg cagctgatgt cgccgacggt gcggaagcgg acatcaagga tttcggaggt
    30361 ttcagacggc attattcctt atttcttctg tctgatattt atgaattatt tgtgcagccc
    30421 gcattctttg ctgtttctgc cttcccacca ccaccgcccg gcgcggatgt cttcgcccgc
    30481 cttgacgggg cgggtgcagg ggtcgcagcc tatgctggga aatccttgcc ggtacaaatc
    30541 gttgtaaggc acattgttgg cgaggatgta tgcccacacg tcgtgttccg accagtcgaa
    30601 aatcgggttg tatttgccga tgtcccgtcc ggcatcgtat tcggcaaacg gcagttccgt
    30661 gcgtgtggcg gactgttcgc ggcgttgccc ggtaagccag gcgtccgcgc ctgcaatggc
    30721 gcggttgagc ggttcgattt ttcggatgcg gcagcattcg cggcgcgctt caacgctgtc
    30781 gtaaaaggca aacctgcctt tgctttccac ataacggtcg gcatcttctc gaaccggccg
    30841 gaaacgcttt atccgcaaat ggggatatgc gcgtccgagt ctgtccagca ggtttagggt
    30901 ttccgtgtgg agcagccccg tatccaaggt aaaaatgccg atattgaggt tttcgccggc
    30961 gataaggtcg gtaatcacca tatcttctgc cgcaaggctg ctggcaaacc gtgcatcccg
    31021 gtggctgccg acaatccggt gcaggcgttg tttgagggtt tccgtttttt ccgcaagggc
    31081 ggtttcgccg ccggatccga tatgcggtat ctgccacagg gcgggtttga acagtgtcgt
    31141 ttccattttt cccgccttat gccgcccgtt gtccggcatt cagtccgccc aatgcgggat
    31201 acgtctgccc gacccggttt tctccttcgc cgttttcacc gaaccaggcg agtttttcgt
    31261 gcagccccac cacttcgcct atgacaatca atgccggatt cggcgcggtt tcggcgagtt
    31321 cggcaaggtt ggcgagcgtg ccggttgcgg ttttttgagc cggcagcgtg ccttggctga
    31381 tgacggctgc tggcgtgtcg ggcgagcgtc cgtgctgttg cagccgttcg gcaatcaggg
    31441 cggctttgag cgcacccata taaatcacca aggtctggcg gctgcgggcg agggtctgcc
    31501 attcgatgtc gggcgcatcc gccttgcggt ggccggttac gaaaaccgca ctttgggcat
    31561 aatcgcggtg cgtgagcggg atgccggcat aggcggtcgc gccgacggcg gcggtaatgc
    31621 cggggacgac cgaaaacggg atctgatggc gtgccaaggt ttccaattct tcgccgccgc
    31681 gtccgaacac gaaagggtct ccgcctttca aacgcaccac gcgcctgcct tcgcgggcca
    31741 gcctgaccat aagcgcattg gtgtcctctt gcggggtgcg gtcgccccgg gcgcgcttgc
    31801 cgacaaaaat ccgttccgca tcgcggcgga cgagggacag tatgccgtct gaaaccagcg
    31861 cgtcgtaaag caccacgtct gcctgctgga tttcctgcag ccctttgagc gtcagcagcc
    31921 ccgcatcgcc gggacccgcg ccgaccagcg agacggagcc gccttgatca ttttgacgac
    31981 tttgttccaa ttggcctgcc aattcccgtt cggcaagggt gttttgccgg tttttgacga
    32041 gggcggcgaa acgtccgtta aactgctttt cccaaaagcg gcggcgttcg gtaacggatt
    32101 tcagtttgcc cttgacggca tcgcgccacc ttcctgaaat ttccgccata tcgcccaaag
    32161 acggcggcag cagggcttcc agcctttcac gcagcagtcg ggcgaggacg ggcgcgctgc
    32221 cggagctgga aacggcaatc tgaaccgggt tgcggtcgat aaccgacggg aagatgaagc
    32281 tgcaatggtc gcggtcgtcc accacgttga ccggcttttg gcagctttcg gcaagatgga
    32341 aaacgcgccg gttgagggct tggtcgctgc ttgccgcaat gatgaggaaa accgtgcgga
    32401 tgtgttcggc acgaaattct tcggcaagcc acaggatttt gttttccgcc gccaacgcgg
    32461 agagttcggc attcaggtgt tttgcggcaa ccctgacctc tgcgcccgcc ttcagcagca
    32521 ggctgatttt gcgtgcggcg accgcgccgc cgcctacgac caatacgggg cggccggcga
    32581 ggttggcgaa aatagggaaa taattcactg gctgactcct ttgctgtttg cccgcacctt
    32641 gtttccgata cggtgcgtcg cggcattttt gtcggaatgc gggtcatttt agacaaaagg
    32701 attttcccgg ttaaataata aaaaggcatt tgttagaagc tgaaagctat atgagggcgg
    32761 ctgcggatac ggcggttt
//
LOCUS       DAXIHS010000014        31575 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXIHS010000014 DAXIHS010000000
VERSION     DAXIHS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.014"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(115..1716)
                     /locus_tag="ACFOF2_000560"
     CDS             complement(115..1716)
                     /locus_tag="ACFOF2_000560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC7106928.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCHALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPKEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDTSGRLKKFDFIVSNPPFKLDFSDFREQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSSIDKKIREYLVENKMLAGMVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAKLEKEIKDRLAMLEFNS"
     gene            complement(1764..2957)
                     /locus_tag="ACFOF2_000561"
     CDS             complement(1764..2957)
                     /locus_tag="ACFOF2_000561"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC7106929.1"
                     /translation="MKRQITIGEIAEINANSLTQKDMFQEIMYLDTGNITRNEIDNIQ
                     VLNVMMDKIPSRAKRKVKDKTIIYSTVRPNQEHYGFLENPGDNFIISTGFSTIDVYDE
                     NTDEKFIYYLLTQKHITDYLHTIGENSVSSYPSINPDDIANLKFAVPDLKTQQSIAAV
                     LSALDKKIALNKQINARLEDMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKR
                     EIPKGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNP
                     QDAHIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYA
                     RHFKFLKEFKIILPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQ
                     VSVAE"
     gene            complement(3084..6088)
                     /locus_tag="ACFOF2_000562"
                     /pseudo
     CDS             complement(3084..6088)
                     /locus_tag="ACFOF2_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6202..7512)
                     /locus_tag="ACFOF2_000563"
     CDS             complement(6202..7512)
                     /locus_tag="ACFOF2_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC7106930.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            7824..8245
                     /locus_tag="ACFOF2_000564"
                     /pseudo
     CDS             7824..8245
                     /locus_tag="ACFOF2_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8331..9031
                     /locus_tag="ACFOF2_000565"
                     /pseudo
     CDS             8331..9031
                     /locus_tag="ACFOF2_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(9112..9176)
                     /locus_tag="ACFOF2_000566"
                     /pseudo
     CDS             complement(9112..9176)
                     /locus_tag="ACFOF2_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9170..9274
                     /locus_tag="ACFOF2_000567"
                     /pseudo
     CDS             9170..9274
                     /locus_tag="ACFOF2_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(9295..10209)
                     /locus_tag="ACFOF2_000568"
     CDS             complement(9295..10209)
                     /locus_tag="ACFOF2_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC7106931.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            10465..11934
                     /locus_tag="ACFOF2_000569"
     CDS             10465..11934
                     /locus_tag="ACFOF2_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC7106932.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            12185..12556
                     /locus_tag="ACFOF2_000570"
     CDS             12185..12556
                     /locus_tag="ACFOF2_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106933.1"
                     /translation="MNKTLSILPMAILLGGCAGGGGNTFGGLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQVEWENKICGCATEEAPNQLTGNDVMQMLNQSTR
                     NQAITTVTAKTVSACFKRLYR"
     gene            12629..14038
                     /gene="thrC"
                     /locus_tag="ACFOF2_000571"
     CDS             12629..14038
                     /gene="thrC"
                     /locus_tag="ACFOF2_000571"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC7106934.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAVLKGLETLPQRV
                     QTAPNSADAVKGIIEQTLA"
     gene            14085..14879
                     /locus_tag="ACFOF2_000572"
     CDS             14085..14879
                     /locus_tag="ACFOF2_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106935.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSVGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            15067..15843
                     /locus_tag="ACFOF2_000573"
     CDS             15067..15843
                     /locus_tag="ACFOF2_000573"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC7106936.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            15919..16025
                     /locus_tag="ACFOF2_000574"
                     /pseudo
     CDS             15919..16025
                     /locus_tag="ACFOF2_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16614..19085)
                     /locus_tag="ACFOF2_000575"
     CDS             complement(16614..19085)
                     /locus_tag="ACFOF2_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC7106937.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(19082..20260)
                     /gene="hflX"
                     /locus_tag="ACFOF2_000576"
     CDS             complement(19082..20260)
                     /gene="hflX"
                     /locus_tag="ACFOF2_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC7106938.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(20265..21545)
                     /locus_tag="ACFOF2_000577"
     CDS             complement(20265..21545)
                     /locus_tag="ACFOF2_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC7106939.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            21547..21675
                     /locus_tag="ACFOF2_000578"
     CDS             21547..21675
                     /locus_tag="ACFOF2_000578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106940.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(21698..22423)
                     /locus_tag="ACFOF2_000579"
     CDS             complement(21698..22423)
                     /locus_tag="ACFOF2_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC7106941.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(22496..23173)
                     /gene="radC"
                     /locus_tag="ACFOF2_000580"
     CDS             complement(22496..23173)
                     /gene="radC"
                     /locus_tag="ACFOF2_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC7106942.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            23200..23322
                     /locus_tag="ACFOF2_000581"
     CDS             23200..23322
                     /locus_tag="ACFOF2_000581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106943.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(23296..24645)
                     /gene="gshA"
                     /locus_tag="ACFOF2_000582"
     CDS             complement(23296..24645)
                     /gene="gshA"
                     /locus_tag="ACFOF2_000582"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC7106944.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWYSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            24905..26314
                     /gene="leuC"
                     /locus_tag="ACFOF2_000583"
     CDS             24905..26314
                     /gene="leuC"
                     /locus_tag="ACFOF2_000583"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC7106945.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            26411..26665
                     /locus_tag="ACFOF2_000584"
     CDS             26411..26665
                     /locus_tag="ACFOF2_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106946.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            26727..27368
                     /gene="leuD"
                     /locus_tag="ACFOF2_000585"
     CDS             26727..27368
                     /gene="leuD"
                     /locus_tag="ACFOF2_000585"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC7106947.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETFAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            27537..28808
                     /locus_tag="ACFOF2_000586"
     CDS             27537..28808
                     /locus_tag="ACFOF2_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC7106948.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            28824..29555
                     /locus_tag="ACFOF2_000587"
     CDS             28824..29555
                     /locus_tag="ACFOF2_000587"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC7106949.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            29589..30659
                     /gene="leuB"
                     /locus_tag="ACFOF2_000588"
     CDS             29589..30659
                     /gene="leuB"
                     /locus_tag="ACFOF2_000588"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC7106950.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            30822..31385
                     /locus_tag="ACFOF2_000589"
     CDS             30822..31385
                     /locus_tag="ACFOF2_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC7106951.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         7722 a         7870 c         8009 g         7974 t
ORIGIN      
        1 tggattctct ctttcaggga aagaacggca taagtatttt ccaaaccaaa caaaatgccg
       61 tctgaaagcc tttcagacgg cattttaagt ttgaccggtt tcattcatcg gtatttacga
      121 attgaattcc aacattgcca gcctatcctt aatctccttt tccaatttgg cagattcggc
      181 gaaaagttta tccaaatccg ctgaaaatcc cgccattttt tgcgcaaatt cgtcggcgga
      241 aatatccaca taatcaatct ttacctcgaa atactgcccc gccgacaggc tgtaattctt
      301 ggctttgatt tcatcgtagc cgaccaccac gctgaaatct tccactgcct gtttgtgcgt
      361 gaaagtattg caaatttttt gttcttcttc gcgggagagt acggtttttt ggtttttgcc
      421 gtctttgatt ttttcgccca agcccgatgc gtcgattaat accacgttgt ctttattggc
      481 tttatcgata aacaggatgg aaacgttggt tcccgtggtg gcgaaaatat tggaaggcat
      541 ggaaaccatg ccggcgagca ttttgttttc taccagatat tcgcggattt ttttgtcgat
      601 actcgattgg gcggtgataa agcccgtcgg caacacaatc gccgctttgc cgttttcttt
      661 caggctaaac aagatatgct ggataaaaag ctggtaaatt tccatcttat ccgtatcttt
      721 tgccttgatt tttgggatac cggcaaaaaa gcgttcgcgg ttttcctcgc cttccaattg
      781 ctcgcgaaaa tcgctgaaat ccaatttaaa cggtggattg gaaacaatga aatcaaattt
      841 tttcagacga cctgaagtgt ctttatgggc gggcgacaaa atcgtattac cctgaaccac
      901 attattcaaa gaatgcacca aattgttcaa aatcagattc agccgcaaaa gattggacga
      961 tttttgcgaa atatcctgcg tataaatcat acatttgtct tcaccgatgg cgtgcgccac
     1021 gttcatcagc agcgttcctg aaccggctga cggatcgtaa acgtccacgc tgcggatttg
     1081 cccacgtacc tcctttggca ccaaaatatc cgccataatc cgcgctactg cgtgcggcgt
     1141 gtagtattcg gcatatttgc cgcccgaatt actgttgtag tctttaatca ggtactcaaa
     1201 aatcgtggcg aaaaaatcaa atttttgcgc aaaaatcgcc tcgaagctga aacccgccag
     1261 tttggaaatc agcgcgtggc aaaaatcgtc gcggcgacct tcatccgtaa tatattgact
     1321 gatacgctcg aacaacacga ttttcgcccc gccttcggtt tttaccgaaa acagttccgc
     1381 attatgtgcg gcaatatcgg tcagcgtatc gtcaaagagt ttggcaaagt ccgcgccgtt
     1441 ttgtcgctct gataaatact tgatggaatg ttcaggcttc aaaacggctg tttttccctc
     1501 aatatccata ttgatgaatt cgattggctc gtcgggattt tctttgcgaa tttgtttaac
     1561 ttcgaaatcg tatttgtcgt ttaggaattt atacaaaaat gcctgactga tgattttgaa
     1621 ctcgttgccg tcattgccca aaccatagtt ggcgcaaatg gtttttaagc tgtcgattag
     1681 ggatttggtt tgttcggtaa agtgttgttc ggtcattttt ttctcgtttg ttttattttg
     1741 taggtctgat tcttgaattt ggcttattcc gccacagaaa cctgcccgtt catcagcatg
     1801 ggaagtagga aatcgcggag ttgggttagt tggtggtttt gttttatatt atttcgaact
     1861 ttaacaaaga aagtatttgc tatttcatta tatttttgag aaatatcttt actaggtaaa
     1921 attattttaa attcttttaa gaatttgaaa tgtctagcat atccataact tgataaatct
     1981 atttgattta tgatttggta aaacaaatag tttggcattc gttcattatt gcttaatatt
     2041 acttgtgtac catctgctcc ccgcgcatat tgaaaattaa ctaacttaac aattcttgta
     2101 tgatcaccaa aaataatatg agcatcttgc gggtttaata tagatttttc atcattggta
     2161 aatccacaaa taaaatcttg actttggtca acaacaggat attttccaaa atccttaata
     2221 tctttattta atatttttgt agtatttggg atttttgcta agcaagattg taattctata
     2281 cttccccacc ccttcggaat ttcccgtttc aaggtttcat caaacacaat ttcgccgcct
     2341 gaggatttgt agggcttgcc gttggcgtca gggaagtcga actgcacgaa ccaatagtcg
     2401 tacagggttt tcgccatatc ttccaggcgc gcattgattt gtttattcag ggcgattttt
     2461 ttgtccaaag cggataggac ggcggcgatg gattgttggg ttttgaggtc aggaactgca
     2521 aattttagat ttgcaatatc atcaggattt atcgatggat atgatgaaac actgttctcg
     2581 ccaattgtat gtaaataatc agtgatatgc ttttgagtca ataaatagta aataaatttt
     2641 tcatccgtat tctcatcata aacatcaatt gtactaaacc ctgttgatat aatgaaatta
     2701 tcaccaggat tttccaaaaa accgtaatgc tcctgatttg ggcgaactgt agaatagata
     2761 atagtcttat cttttacttt tctttttgca cggctaggta ttttatccat catgacattc
     2821 aaaacttgga tattatctat ttcatttcta gtaatattgc cagtatccaa atacatgatt
     2881 tcttgaaaca tgtctttttg agtgagggag ttggcattta tttctgcgat ttcaccaatt
     2941 gtaatttgtc gtttcatctt aacttaatct ctcgaaactc ttcaaaggcc gtctgaaaaa
     3001 aatttcaaaa tccaaccgca cttttattgt ccacttcgaa cgtatgcaat acgcccataa
     3061 aggctaccta aaaaaccgct ttctcacatc cgcccgcttt cttttaaata ttcccgcacc
     3121 aacaggcggt tgatggcttg gatgtcttgg ggttgaacag gaaattgttg ctccgttcta
     3181 aagcgtttta atacgatgcc ctgcatttgt ttttcaaagt aattttgatt gtccaaaatc
     3241 tgctccatat ccagcacttt ttggtcggca tcggctttga cgccgtttag agcgttaaaa
     3301 actttgagtt tgtcgccgta gaaggcggcg ttttccatca gccgcttgtg ggtgcgggca
     3361 tacttagcgt cgccgccgta tttgtgccgc aaaaggtcgt tttggcggtt cagctctttg
     3421 attttggcat agacgatttg gagtgtggcg atgtttgcct gcatttcttc ttgcccgact
     3481 tcggcgaggt tctttttctt gaagatgcgt tccagctctt cgcgcaggct gatgtattcg
     3541 gggtcgtctt ggtcgaagtt gcccgccagc ccttcacgga ctttgcgcat gatgtctttt
     3601 aaatcgtcgg caagtttgag ttccgcctcg ccgattttga cgaattgaaa gtaaacgtct
     3661 tccagcgctt cattgagcaa atgggcggtg tcgccttgct gtaatttttc ggcaaggttt
     3721 aaggtatcca gacgggcggc agtttcgcgg tacagaaggt tgagcttgtc aaaatccaga
     3781 tgcacaagaa attcgtggct gccttgcagg cgcagagcgt tgtatagctc tttggaagtt
     3841 tgcagggctt tttttagggc gagaagctgt tttttgtctt ctatttggct aatttggctg
     3901 caaaattctt cggcgttttc ggtatcgaaa tcaaacaggg cgtttttaat gtctgcaatt
     3961 tcctgttcga tttcttcggc ggttttgaat agctggctgt aactgccgat ttcgtcgccc
     4021 aattcgttag aaagttcgtc ccaataggcg cggttggttt tgtcaaattc gcgttcgatg
     4081 tcggcaaaat cgacaacata gccgtaacgg taagatttgt aggtgcggtt gacgcgggtc
     4141 agggtttgta aaaggttatg ggctttgatg agtctgccca aatagagctt tttcaggcgc
     4201 ggcgcgtcaa agccggtcaa aagcatgttg tacacaaaca ggatgtcggt tttgcctgct
     4261 ttgaaatctt tgacccattg gtcgcgttcg tctttggtgc cgacgtcgtg caatatcagt
     4321 gcggcggtga aattatggtc tgaggccgtc tgaaaatgct caaaaagctg gcgcgcctgt
     4381 tctgcgctat cgcaaacgac catggcgccg aggctctcgt cttggttggt ttttctgaat
     4441 ttggcgaagt cgcccaagat gtaatcgagc atcgggcgga cgaaatgcgg atgggcgtag
     4501 atttctttgc ggtcaaagct gcctttttcg acttcaagct gcgccagtgc agcctgcaat
     4561 tgggctttgt attggctgct gatggcttcg cgaatcaggc gcagggtgta gccgtcggcg
     4621 atggaggcgt tgtaatagta tttatggatg tagtcgccga agagttcgcg ggtattgacg
     4681 ttgcctgcgg ttacgccgat aagcggcgtg ccagtcagcc cgattttgac ggcgtttacg
     4741 tcggactggt tgaggttggc aagaaacgag cctttggggt tgtagctgcg gtgtacttcg
     4801 tcgagaaaat agacgcgctg aatggcgagg tcgtagtcgt tacgagcgac gacatcgggg
     4861 tcgtcttgga atttttggat attaacaaca gtgatttccg ctttgcctgc gtggttgtgc
     4921 aaagtttggg cggatttgat gtcggcagca aaggcttcgc ggctgtcgat ggtatggacg
     4981 actaaatcgc gggcagtaaa ttcgcgctgc gcctgtttca ataaatcaag cctgtccacg
     5041 atgaaataga atttcggcac gatgccctgt ttggcataat agtgggtcag gtagcgggtg
     5101 ttgtaatagg caagcgcggt tttgccagag ccttgggtgt gccaaatcac gccttttttg
     5161 ccgccgtttg ccaaatgttt ttcaatggcg agagtggcaa atagctgcgg ataccgcatg
     5221 atgtgttttt gcaccaagcc ttgacttgct ttgacgtaag tcagcccgtg ttgcagaaga
     5281 aaagaaagac gttcgcggca aagcagcgaa gtcaggatgc ggttggtcgg cgtatcgggc
     5341 gatttattgc tgataaattc ggggctgtgt ttgataacgg gcaggttgtt gtcttgcagg
     5401 acgttgtttt cgaggccgtc tgaaagcgtg ttcaataatt cggcaagatt taatttatgt
     5461 tcttcgcgga agtagttaaa cacgggctta ccgtaagcag acgaagcgta aaacgcgcct
     5521 tgtgccggtt cggttgcgcc gtcgtcgtat tccatgttgt tggaaaaaat catgaattgg
     5581 gtgatgttga tgaaacggcg aaatttggaa tttttcgcac gtttgcccat gcgctcccgc
     5641 tcttcgccga ttccgccttt gttgttgggc tttttgactt cgataaaaac caaaggcatg
     5701 ccgtttacca gcagggtaat gtcggggcgg aattcttcat cgccgttgat acagggcaat
     5761 tcggttacaa cgtggaagct gttgttatca aaattttgga agtcgatcag tttcttgccg
     5821 ccggattgat tggtaaggcg ttcgtaaaac ttttgcccaa gtcttcgttg tccagttcta
     5881 agcgtatgtc ggtcagcaaa cggcgtgcat cgtccggcga caaatcggga ttgatgcggc
     5941 agaggctttc tataaagatt ttgggaaaga tgttggttgg tttatcccaa ttttcgtttt
     6001 tgagcgagag gtagtcgtag cccaagcgca tcaggtgcaa aacggcgggg attttgacgc
     6061 gggaattttc gttgtgagtt tgggacataa acgtaatgat tcatcaaaat ttattgaaac
     6121 ggattataac acctgaaaaa cccgatacgc ctttttggtg taccggcttt gccataccgt
     6181 tctgcttcag acggcattgc ttcattttgc ctttaatgcc tcttcatcca gcgatttgag
     6241 ccacgccagc ttttcgccga ttttgatttc caacccgcgc gggacgggtt ggtagaagtc
     6301 cggttcgtcc aagccgtcgg gcatatagct ttcgccggcg gcgtaggcgt tcggttcgtc
     6361 gtgggcgtag cggtattcgc gtccgtagcc caattccttc atcagcttgg tcggggcgtt
     6421 gcgcaggtgg acgggcactt cgtcgctagc gttttctttg acgaagtggc gcatttggtt
     6481 gtatgccttg tagcccgcgt tggatttcgc ggcggcggca agatacaata ccgcttgcgc
     6541 caaagccagt tcgccttcgg gcgagcctaa gcgttcgaag gtggcggcgg catcgttggc
     6601 gatttggaag gcgcgcgggt cggcaagccc gatgtcttcc caagcgatac gcacgatgcg
     6661 gcgggcgagg tagcgcgggt cggtgccgcc gtcgagcata cggcagaacc aatacagcgc
     6721 ggcgttcgga tgcgaaccgc gcacggattt gtgcagggcg gagatttggt tgtagaaact
     6781 ctcgccgcct ttgtcgaaac ggcggatttg cgccccgaga ctgtcggcga gaaattcggc
     6841 ggttaagttt ttcagacgac gtgtatcggc ggcgcgtaaa agttgttcca acaaattcaa
     6901 caatctgcgc gcatcaccgt cggcggtatt cacgagtaat ttttgcgcat ccgtttcaat
     6961 cgtaaactct tggtattcag gcaaagccaa taccttggca atcagctttt tcaggtcgtc
     7021 tgaagacaag ggttgcaaaa catacacctg agcgcggctc aacagcgcgg gattgacttc
     7081 aaacgacgga ttttccgtcg tcgcaccaat aaaggttagc aaaccgcttt cgacatgcgg
     7141 caaaaacgcg tcctgctgcg ccttgttgaa gcggtggact tcatcgacaa acaaaatcgt
     7201 cgcgcgtccc tgctgcaaag cgatttcggc tttatcgatt gcctcgcgga tgtccttcac
     7261 gccggaaaat acggcggaaa caggcaaaaa ctgggcgttg aaactctgcg ccaaaatccg
     7321 cgccaacgtc gtcttgccca cgcccggcgg cccccacagc aacatagaat gcggcttgcc
     7381 gccttctacc gccacgcgca aaggtttacc ttcgccgatg aggtgttcct gccccaccac
     7441 gtcgtcaagc gtatgcggac gcaatcgttc ggcaagcggc gcgtcgggtt ctcgggcaaa
     7501 caaatcggtc ataacggctc cgtcaacagg ttttcaaaca atatgattat acggcaggga
     7561 acggcggcgt gccgcatacg gattccgccc ctccgtttgc cttaagccga tattaggcgc
     7621 atactggaaa agacgagaga cttcacacaa tatatccggc acggagaccg attccgcatc
     7681 ggcatgacaa tacccaaatc agcgtttcaa ttaaacatta aggagactaa aatagaaaat
     7741 ttgctttatc taccattgct ttgttgattc aatcggcatt atgttttgag gcggaagccc
     7801 atgaatatac taatattcaa gagatggaat gggtgtcttt attttctgat ccgcaaagag
     7861 acgatgatag tcttataacc cttaaagatg aaaaaatcac tgtaaaaaac tatattgtgc
     7921 cttggtggaa aaaaggtgaa aactttagaa aattagaact tattaccact ggtaaaaatg
     7981 ataatggaaa tttaacggta aatggaattt acggcatcca atcttttgca gactctcaat
     8041 tgggatatac gcctgaatta ttggtaaaaa gcaataatga tattctattt aaaaataatc
     8101 tccaatctgt tgacgtgaag ccccattccg gaaatatcca tttggatgcc aaaggaaaaa
     8161 ttcaatttaa tttgaatgtt cccgatgttg atggggtaca agatgtagcc agtgaaaaaa
     8221 ataccgacaa acaatagtgc cttagttcgg tatgtcaaca ctatcagccc cccgatgaaa
     8281 atgacaaagg cagcatatcc tttcattcaa aaacaggcaa tttgttcaat atgaacgtta
     8341 aagttcataa agacacacac catttcggta ttcacaacct caacaatacc gatgttcatc
     8401 tgactgccga agacagcgat aatgtgattg agattaaagg ggaactcgcc gaccctatgc
     8461 cgacaggaaa tgttgagagt acaagttttt taaggggcat tgaaacggct tttaaatcca
     8521 ataccgtatt aacggggaat aacaataaaa tatccatcgc cactgagggc aagggcttgg
     8581 gattgggcgt aaatggtctc cagtccaact ccccctattc cggacacacg acgggcattt
     8641 tcatcaataa tcaatcaagc gaccttaccc aaccttccct tgatggcatc agcaccatca
     8701 aactcaaagc cgtcaaggga aataatgaag ttgatttaaa tgtaaaaaac cacgcctccg
     8761 taaaaggcat tatcgcgtcg cattctgcaa aagcaacgct tgaagccgaa ggggacaata
     8821 ttgtccaggt taaaaatccg gacaccaata cggctttaat cgatgctttg aaaaaacaat
     8881 accccaatgc ttcgaaagaa ctatacaggg tgggggttca atccacgact ggaacagtac
     8941 gcatttcgcc aaacggcaaa atcgcattaa cggaattgaa aacttttgga attgtacaaa
     9001 acggcatttg cgtcagttta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt
     9061 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat
     9121 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataacggcat
     9181 ttccaaaggg cattttaagc cgtatttgag agaatgcgga tggcgttttc agtgttttca
     9241 gcgtgataaa attcaaattt ctattttaaa acaatcagca aagcaggatt tatttcagtt
     9301 gtccgagaca acgggcggct gcaccgcctg cactccccat actttttcaa tgcactcgag
     9361 caggagtgag acgtggggat tatcctcgta ttcttccgca taaatgaaat tgagcggcat
     9421 actgtggcgg cacgattcga taatagccac gcccgcgcct tcttttgccg ccgcttcggc
     9481 tttgtttccc ggcaccattg ccacacctat accgcctgca accaaatcga taatggtttg
     9541 ggggtagtcg cacaagatct gttttttggg tgagagccgg ttgctgcgcc aaaactggtg
     9601 caggtgcttc ctacttccgg acacgcccga catttctatc catacgcatt cttgcaggct
     9661 ctgcggaagg gagcgggtca gatggggata ttggctttgc gggcaaatca gcgcgtaggt
     9721 caggttttgc aggaatacgc tgcggatgcc gcgttgggcg gcgttgccga gtataaagcc
     9781 gccgtgcagg gttttgtgtt ggatgcgcga gaggatttcg ccgctcattc cgtattggat
     9841 gtgcaggcgc gttttggggg ctgtttgacc gatattgtcc gtcagcgcga cgagttttgc
     9901 cgaatcgatg gggtggataa tgcccaaact ggtctcttcc gaataatcgc ctgccagcgt
     9961 tttggcaaaa tgctccagct tgtgtttgta ttgcagcagg gattccgctt cgggcaacag
    10021 tatttcgccc gcccgcgtca ataccatgcc tttccccgtg cgcctgaaca gcggcgtgcc
    10081 gacatattct tcaagggctt taatttgggc agaaacggca ggctgggaaa ggaaaagtcg
    10141 tttggcggct tgggtaaggt tgccctcgtg cgcgacggcg acaaatgatt ttaattgtac
    10201 ggcatccata tatccctcct tgtgcggatg ttttctatat ttgtgcaatc gaaatctttt
    10261 aggtggattg ttgctgaaaa ttaacttttt aatcaagtgg tttgtaaatt gtatcagttt
    10321 tccggatgat ggttatcaaa aaaaaagatt ggttttattg cctgtttggg ctttaaatgg
    10381 ggttacggct tccgaacgca gcccgtatca aaaagaaaag tcatgcgccc cttttacgag
    10441 gcgcgatata taaggaggaa ggttatggaa aaacataatg ggacttatcg ggatttgcac
    10501 cgtccggctt cggaatttgc gacgcgggac gaatatttgg aacatgaatt gcagattatg
    10561 caaccaaaac gctggcggcc caacctgccc tttcgcgatt accgcttcga gtgggaggat
    10621 ttgattcctg cgatggcggg aacgattgga aaagtggtga tggtgggggc ggtggcggcg
    10681 gcgtttgccg cacctttggg gctgcctgac agctttgtac tggaaaatgt gcgctatgag
    10741 cttttaatcg ccgccgcgtt tatcttattg gtatcgggct tttttctgcc cggcgccaac
    10801 ctgcccggta cgcacgggcc gctgattccg atgattccca tcgttgtgtc ggcaggcggg
    10861 catcctttgg cgttcggcat ttcgattgcg gttttaggtc tgctgatggc tttatttcgc
    10921 ggcggcagta ttatggcgaa gctgacaagc aacggcgtat gcggcggatt attactctat
    10981 ttgggcttta tcggcacgac ggggcaggta aaaaaattgt tttcgtgggc aggcggtttt
    11041 aatatgccct acatcgcttt taccgtcatt attgtaacga ttgtgatgta cgctttgttg
    11101 gagcattgga aaaaacgctg gttagccgtg cctttgggat gcttgattgc cggtgtggtg
    11161 gcatttgcat tgggtgcgcc gtttgagttt cacaccgccc ccggcctgcc tccaatgagt
    11221 cctgcttatt ggtggggtga aaacagcggc tggcatctgg ggttgccgac ggcagaaagt
    11281 tttttggttg tctttccatt tgcggtattg gctgttgcaa tgtggtcgcc cgatttttta
    11341 ggacatcaag tgttccaaaa aatcagctat ccggaaaaaa ccgataaggt attgatgaat
    11401 atagacgaca ccatgacaag ttgttctgtc cgtcaagcag tgggttctat tttagggggt
    11461 gcaaatttta cctcttcttg gggaacttat atcgtaccgg catcgattgc caaacgcccc
    11521 attccgggcg gtgcggtttt aacggcggtt ttatgtatta tcgccgggtt gtggggctat
    11581 ccgatggact tggcgatttg gcagccggta ttgagcgtag ccttggtcgt aggcgtatac
    11641 ttaccgcttt tggaagcggg catggaaatg acgcgcaaag gcaaaaccac ccaatccgcc
    11701 gccatcgtgg tgttctcttc cgccttggtc aatccggttt tcggctgggc gttgacgatg
    11761 ctgttggata atttgggctt aatcggctgc aaagagcgca gtgcgcaatt aggtttcgcc
    11821 ggacgcgtgt tgatacccgc agtaggtttc ttgatcttgt gtgtggcgat gggtgcggtc
    11881 gggatgctgc ccggtatccc gccgtttttg gaacacttca aatctttggg ctaggctgaa
    11941 atcggaaatg ccgtctgaac cgctttcaga cggcattttt gcaaacaggc aaaatgacgg
    12001 cggcgggatt ttttattttc ccaattgaag tataatgttg ccgacttcaa ccggatattc
    12061 aaacggtttg ctccaacact cggaacggcg cataaaacgc ggccctgcgc gttatcccga
    12121 acggggcggc taatcagatc ctatcgccat aaaacggcgg ggtttcaacc gaaaaggaat
    12181 tgagatgaat aaaaccttgt ctattttgcc gatggcaatc ttactcggcg gctgcgccgg
    12241 cggcggcggc aacacattcg gtggcttaga cggcggcacg ggtatgggcg gcagcatcgt
    12301 caaaatggcg gtagaaagcc aatgccgtgc ggaattgaac aaacgcagcg aatggcgttt
    12361 gaccgcgctg gcgatgagtg ccgaaaaaca ggtggaatgg gaaaacaaga tttgcggctg
    12421 cgctaccgaa gaagcaccca accagctgac cggcaacgat gtgatgcaga tgctgaacca
    12481 gtccacgcgc aatcaggcaa ttaccaccgt taccgccaaa acggtttccg cctgcttcaa
    12541 acgcctatac cgctaacctt cttcagacgg cataaggcag atgccgtctg aagccgtttt
    12601 cctgcaattt cgacagacga gaaaaatcat gaaatatatc agcacgcgcg gcgaaaccgc
    12661 acacaaaccg ttcagcgaag ttttattgat ggggctggca cccgacggcg gcctgatgct
    12721 gccggaacat tatccgcaaa tcgggcgcga aaccttggac aaatggcgcg gtttggctta
    12781 tcccgaattg gcgtttgaaa ttatgcgcct gtttgttacg gatattccgg aagacgattt
    12841 gcgcgatatt ctgaaccgta cttacacgga agcggcgttc ggtactaagg aaattacccc
    12901 cgtccgcacg ctttcagacg gcatcaaaat ccaagccttg tccaacggcc cgacactggc
    12961 gttcaaagat atggcgatgc agtttttggg caatgcgttt gaatatgttt taaacaaaga
    13021 aggcaaaaaa ctcaatatct tgggcgcaac cagcggcgat acgggttcgg ctgcggaata
    13081 tgccttgcgc ggtaaaaagg gcgtgaatgt atttatgctg tcgcccgacg gcaaaatgag
    13141 cgcgttccaa cgtgcgcaaa tgtacagcct gcaagacggg aatatccaca atatcgccgt
    13201 gaagggtatg tttgacgact gtcaggatat tgtgaaggcg gtgcagaacg atgccgcgtt
    13261 caaggaaaaa taccacatcg gtacggtcaa ttctatcaac tggggacgca tcgtcgcaca
    13321 agtggtttat tactttgcgg gctatttcaa agcgacgcaa agtaatgacg agcaagtcag
    13381 cttttgcgtg ccgagcggca acttcggcaa cgtttgcgcg ggacacatcg ccaaacaaat
    13441 gggcttgccc atccgccgcc tgattgtcgc gaccaatgaa aacgatgtgt tggacgagtt
    13501 tttcaaaacc ggcgcatacc gcccgcgcaa cagcgagcat acttatgtta cctccagccc
    13561 gtcgatggac atttccaaag cgtccaactt cgagcgtttc gtgttcgacc tgatggatcg
    13621 cgatcctcag gaaatcaata cgctgtgggc ggaagtggct gcaggcaaag gctttgacct
    13681 gcgatttgcc ttggacaaag tcggtggcaa atacggcttt acttccggca agtccaccca
    13741 cgccgaccgc ctcgccacca tcaaacaggt ttacgagcaa gaccaagaac tcatcgaccc
    13801 gcacactgcc gacggcgtaa aagttgcccg cgaagtgcgc gaagaaggtg aaacggtggt
    13861 ttgtttagaa actgcgttgg cagcgaaatt cgatgcaacc atacgcgaag ccgtcggcga
    13921 tgtcgccatt ccgcgccccg ccgtgctgaa aggtttggaa acattgccgc agcgcgtgca
    13981 aaccgcgccg aatagtgcgg atgcggtaaa aggcatcatc gaacaaaccc ttgcctgatg
    14041 tcccgtgtgg gcggcattcc gtccgaactt aagattgaaa gaaaatgacc ttacctatgc
    14101 aggaaacccg tttttctata ctgctggacg aactggcggc aaaacaggag gcagccatcg
    14161 ctccccacct gcttgccgac ggaaccaagg tatgggtacg caaagcaggc aggcacaatg
    14221 cgcggtggcg ttacgcgctg ctcggtatgg ttgcccgata tttgaaactg ggggtgttga
    14281 agccggttcc cagcctcggc ggcgagcctg ccattgcaac cgaatcaaaa cgcctgtacg
    14341 aattgcgctc ggtggggata gccgttcccg aattgctcgc cgtcaggaaa aatgccctta
    14401 tgttcggcaa tttggcaggc atcccgctcg atgcgcaaat ccgccaagaa gccgaagccg
    14461 gaaaggcgga cgcttggctt gccggtttgg aagccattgc acgcgtgcat aaaaaacagc
    14521 agttcctcag tcaggcgttt gcacgaaata tgatgtggga cggaaaaaac atcagctttc
    14581 tagattttga agacgaccct tccgaagtcc tgaccatagc gcaatgccaa gcccgcgatt
    14641 ggctctgcta catccattcg accgcgctga tattgaaaaa cggcggactg ctggaagcgg
    14701 ctgcggaaaa atggggcggc gtactgtctg agcagcctgc cgaaatacaa aggctgattg
    14761 ccgccaccgt caaaccgatc ctcccgatac gcaggctgga acacccccgc tggggacggg
    14821 acgcgctcag gctggcagcc tcgatttccc tgatttccct tgccgatata ccgccataag
    14881 ccgccgcccg ccccgcgatg ccgtctgaag cccttcagac ggcatataac caacgcgcat
    14941 caagcattgt cgcaaccatc cgtttcgttt agaattttga ctttatcccg aatcgggcag
    15001 acattttcac tctgccgcac atcccgaaca agcactatcc atttctttta ctgacaggaa
    15061 acccaaatgg cagcattcaa tacccaaaaa gtattgtccg tacaccactg gacagacgca
    15121 tattttacct ttacctgcac ccgcgacgaa tcgttgcgct ttgaaaacgg acagttcgtt
    15181 atggtcggat tgatggtgga cggcaagccg ctgatgcgcg catacagcgt cgcctccgcc
    15241 aactgggaag aacacctcga atttttcagc attaaagtcc aagacggtcc gctgaccagc
    15301 cgcctgcaac acctcaaagt cggcgacgac gtgttaatca gcaaaaaacc gaccggaacc
    15361 ctggttgccg gcgacctgaa tccgggcaaa cacctttact tgttgagcac cggtaccggc
    15421 atcgctcctt tcttgagcat caccaaagac cccgaaattt acgagcaatt tgaaaaaatc
    15481 atcctcgtac acggcgtgcg ctacaaaaaa gatttggcgt actacgaccg ctttaccaaa
    15541 gaattgcccg aacacgaata cctcggtgac ttggttaaag aaaaactgat ttactacccg
    15601 attgtttcgc gcgaagaatt cgaacaccac ggtcgcctaa ccgacctgat ggtgagcggc
    15661 aaactgtttg aagacatcgg cctgcccaaa atcaacccgc aagacgaccg cgccatgctg
    15721 tgcggcagcc ccgcgatgct gaaagatacc tgcaaagttt tggacgattt cggactgacc
    15781 gtctctccga aaacaggcgt acgcggcgat tacctgattg agcgtgcgtt tgtagatcaa
    15841 taagctgttt gaaaatcccg tctgaacggc accagcaatc aaaaaccccg aatcctgcca
    15901 atcaggattc ggggttttta tagtgaatta aatttaaatc aggacaaggc ggcgagccgc
    15961 agacagtaca aatagtacgg caaggcgagc caacactgta ccggtttaaa tttaattcac
    16021 tatatagtgg gtaaaaatta gaaatgcaca tttccgtcat tccaacacag gtgggaatcc
    16081 ggacttacct gtttcattat cgtttgaaaa taaaagagaa accaaacata tagattcccg
    16141 cttgcgcggg aatgacgacg gtgttttgcc gtagccaaag cagaaaaaca tccctattgt
    16201 catttcatca tgatatgaca ataggatgtt aaaaattaat tatcattata tgattgatat
    16261 ataagaaaaa ctgtcccaat aagaaatgtt atgcagaaaa taaaatgaaa aggatggttt
    16321 tcattgaaaa tggttgttct gccaccgtca tacacagagc cagtatttgg aaaaaaatga
    16381 tccatattga catcattatt ccatttgtcg cataaatcca ctttttaatt ttttccacat
    16441 taatataatc tcttatttaa taaaatcaaa ataaaaatca aatgcctaac aattcgatct
    16501 caaattttga acgccaacgt gtttgagcgg taacaataca tactttaagt ttgcaattct
    16561 tcatcgtctt atttttgttt tcatcaacta tacgtctaaa gcaacgccgt ccgtcattgt
    16621 tcggacggca ttttttcaga ctgcattttc ccccgtttgt gaaggcgcag ggcgttgccc
    16681 aaaaccgcca gcgaactgaa gctcataccc agtgcggcta tccacggttg gacatagccc
    16741 aaaacggcaa gcggtacggc aatgatattg tacgcgcccg cccatatcag gttttgccgg
    16801 ataatatggc gggtgcgccg cgcctgatcg agcaggtggg cgacggtacg caaatcttcg
    16861 ttcaataaca caatgtccgc gccgtccctc gcaatatccg tcccgcccgc tgcggcggcg
    16921 gatacgtctg cctgcgccaa aacgggcgcg tcgttgatgc cgtcgcctat catcagcact
    16981 tttttccctt ctttttgcaa ggctttgacg tattccagtt tgtcctcggg catggcttgg
    17041 gcgcggtagt gcgcgacacc cagggcgcgt gcggtttcgg caacggcggt ttcgcggtcg
    17101 ccgctgagga tgtgcagggt caggtttttg cctgccaact gccgcaccgc ctccgccgcg
    17161 ctgtctttca aggggtcggt caggtagaac acggcttgga aaccgctttg actgccgagg
    17221 taaaccgcgc tgccgccgcc ttctgtttgc ggttctttac ctgaaatttc ggcgacatag
    17281 gatgccctgc ccaatgccca aacctgtgtt tcgccgttga cggtcagttg cgcgcccacg
    17341 ccttcgccga tgcggttgag gcgttgttta atagcgatgt cggggacgct gccgtctgaa
    17401 atgcggcagt tgaggatggc gcgggcaagg ggatgttcgg actgttgttc taaagcctgc
    17461 gccaccgcga gaacaaaggc ttcgtctgtg cctctcaaca atgagatacg gcggacggcg
    17521 ggtttgcctt gggtcagcgt gccggttttg tcgaagatga tgtcggtggt ttgggcgagg
    17581 gtttcgattg cctgctttcc gccgattaaa ataccttcgc gcgccagcgt accggtagaa
    17641 gctgccagcg cggtcggcgt ggcaagcgat aaggcgcagg ggcaggtaat gaccagcagg
    17701 gcgacggtaa tccacaatgc ggtgtgcgcg tcggcgtaca gcgtccagcc gatgaagacg
    17761 gggacggcaa gcaggagttc gccgaatatg aaagacgagg cgtattgttc cgccaactcg
    17821 gcagtgcgcg gtttttgcgc taaggcgcgg tcgagcaggc ggacgatgtg cgacagtcgc
    17881 gtgccaccgc cggtgcggtc ggtgcgtata atcagggggc tttgcgtgtt gagtgtgccg
    17941 gcggttactt tttcagacgg cattttggcg acgggcaggc tctcgccggt cagcatagat
    18001 tcgttgacgg cactgcttcc ttccagcacc gtgccgtcaa cggggatggt ttcgcccggt
    18061 ttgaccagca cgatatcgcc cgccttcaat ttgacgacag ctgcctcgca ggtttcctgc
    18121 gtatcggggt aatcgggcat atgatggcaa aacgcaggaa tcagcttcac cagcctctcg
    18181 gcggcatcgc ctgccttacg gcgggcaatg tgttccataa agcgtccgcc cagcaggaaa
    18241 aacagcagca tcgcgatgga ttcgaaatac atcccctgcc ccgcatttgt cgcaaggctg
    18301 taaacgccgg cgataaaggt catgatgatg gcgacggtaa tcggcgtatc catgccgacg
    18361 cggcggtttt tcaagtcgcg cagcgcgcct tgataaaacg ggacggcgca atagaatacg
    18421 acgggcagca ccattaaaaa gccgccccaa tggaggattt gcaggaaatc gggttcgatg
    18481 tcgccgccgt aaaggtaggt cggcagcgcg aacatcatcg tctgcatcat ccccagcccg
    18541 gcaacggcga ggcggacgat gtattgtttg cgttcttttt ggttggcggc ttcgattttt
    18601 tgcgcgtcat agggtgcggc ggtgtagcct atctgcctga ttttcaacag aatgtcggaa
    18661 aggcggattt tgccgtcgtc ccagacgacg cggcagcggt gcgtgctgta attgaggtcg
    18721 atgcggacga tgccgtctgt acgcaaaagc tgctgttcga tcagccagac gcaggcggcg
    18781 caggtgatgc cgccgagcat taaaaccgcc tcgcgcgtgc cgccgtgggt ttccacaaag
    18841 tcggactgga cttcgggcag gtcgtacagg cggatttggt cgaggatttc ttggggcggc
    18901 agctcggttt tttgcgcgtc ggcggtgcgt tgtttgtaat aactgcccaa gcccgcgtca
    18961 ataatgcttt gtgcgaccgc ctgacagccg gcgcagcagg tttcgcggtc ttcgttttcg
    19021 taacggacag tcaggtggag gtgttcggga acatccagac cgcagtggaa acaggttttt
    19081 ttcatggcat ttcggtttcg tctgtgtttg gtgcggcggc acaagactcg gcaatggctt
    19141 cgcgcagtgc gtctataccg gtattttcag caacggaaat gcggacggcg gcaatttttc
    19201 ccgcagcgtc gcgccatatg cccgtgtttt gttcttcaga cggcagcagg tcggttttgt
    19261 tgtacacctt gatgcacgga atatcgccgg catggatttc ttgcagtacg ttttccacgt
    19321 cttcaatctg ctgtccgctg ttcggagcgg cggcatcgac gacgtgcagc agcacatcgg
    19381 cttgcgcggt ttcttccagc gtggcggaaa aggcggaaat cagtttgtgc ggcagatcgc
    19441 tgacgaatcc gacggtatcg gtcaggataa tgctgcattc gggactgatg tacagccgcc
    19501 gcgccgtcgt gtcgagtgtg gcgaaaagct ggtctttcgc atatatgccc gacttggtca
    19561 gccggttgaa caggctggat ttgccgacat tggtatagcc gaccagcgca aacgttttga
    19621 ttgtgcccga ttcgcgggac ttgcggcgca gggcgcgctg ttttttgagg ttggcaagct
    19681 gttttttcaa ggcattgatc cgatgggcga tcaatcggcg gtcggtttcc agtttggttt
    19741 cgccggggcc tttcatgccg ataccgccgc gctggctctg cagatggccg taaccgcgta
    19801 tcaagcgtcc cgccaaatgg ctcaattgcg ccaactcgac ttgcagcctg ccttcctgcg
    19861 tgcgggcgcg gcgagcgaaa atcgccagaa tcagccctac cctgtccaat acgcggcatt
    19921 tcagttcttt ttcaaggttg cgttcctgcg tgggcgtgag ttcgtggttg aatacgacca
    19981 aatcgatgcc gtctgcggca actgcttctg acagctccgc cgccttgccc gtgccgacaa
    20041 acagcgcggt gtgcggacgg tcgcgtttgg cagtctccac gcgcacggaa tcgccgcccg
    20101 ccgctttgac cagctcgaca gcttccgcca atgccgtctg aaaaccgttc agacgggcgg
    20161 cactgctgcc cgtaccatct ttgtccaaca ttacgcccac cagcatgacg cgttcgggtt
    20221 gcgcttgggt actgccgttg ccgcctgttc tgcctgtcat gtattcaatc ctgtaaataa
    20281 agggttacgc ctcgttccgt ttctccttcc ggtgcaagcg tgatgcggct gcccgccatt
    20341 ccgtcaacgc tgaagacgat gccgcccgcc tcgtgccttc ctatggacag gcggctgccg
    20401 ggtcgctgcc ggtaggtata acgggtacac gcttccaaac accagcgtcc gcgccagtgg
    20461 atgaaatatt tttcgccttt tctaaccgca agtgtctcgc cgccgtttac ctcaaaataa
    20521 gggtaatcgg gttttcgggt tctaaactgc catttcgcct gcgcgcgccg tccgttgcgg
    20581 acatagtcga ataccgccgt ataaagcgtg ccgtattcca aaggcttgag cggaaaaagc
    20641 gcgaattggt acgcggtcag cctgccgttg gggtcgttgc cggcggttaa aaccctgacg
    20701 gggcggattt cgtttttacc ctgatacagc ttgaaacttt tcatcgtaat tttgcctgcc
    20761 gcctcggaaa aatcaatgct ggcaggattg cccgtgattt catattccgg cacggggtct
    20821 ggacgctcgc cgtggaaata aggcagtgcg ccgctgccga cgggataggc tgtatagagc
    20881 agctcctgtg cgggcatggc ttcgtcggtg tacacgaccg caccgttatg gcaggcgttg
    20941 cggtaatatt tccgtcctgc ttccggctga tttctgcctt gggcgcaatg ccgctcaaac
    21001 ctgctgttgc cctgattgaa tacgagaacg gttttaccgt tttcgcgcac aaatgccgct
    21061 cctgcctcat ccgtatggcg gtcaagtagg gaaaggcggt ggtagattgc gctcattaat
    21121 ccgtccactt ggcgttgctg cgtgcggatg tcgctgtcgg acgattcggc ggcttcctct
    21181 tccgtgctga tgttttcatg cacgccgttg tagagatacc cggcaaggcg tgtgcgttcg
    21241 gtcagctttt gtgcggtgta gtgcggattg tcgggatggt gttcgccgtg tccgtcttcg
    21301 ggattgagcg tgaggtagct tgcgtgcctg cgggcggagt tttccaaaac cggcgcgtgt
    21361 gccagcttgt gcaaaccgat ttgggctcgg atgcggttga ggtagtgcag cgcatcgccg
    21421 ccgtccctga tttgcggtgc ggacggatag acaagttcgc ccgcgggcag ggattggttt
    21481 tgggtatggt agaaaacgcc tgccgccgaa cccaacagga gcagccgaat aaaaagggat
    21541 ttcataatgc cgtcctcccg acgcggcaac ggggtgtttt atcaaaacgg caaacttgcc
    21601 aatgcggttt ccgattgcag attgccaaac cggcaaacct ttccccgtgc cgatgccgaa
    21661 cctgatgccg tctgaacctt cagacggcat cggatgttta tttgtccacc cggatgggtg
    21721 caggttcata ttgtgtcgat tcgtcgccgt aatacagcac gccgagtttg atggggattc
    21781 tgccctgcga tttgcggtgg gcattggaat cgcgcaagga ataggcgcaa ccgcaatatt
    21841 cctgctggta gaagttttca cgtttgctga tttcaatcat gcgcgcgctg ccgccgcctt
    21901 tgcgccagtt gaaatcccaa tacaccacat catcgtaagg cgcggcggcg cggtgtccgc
    21961 agtcgttgat ttgcgccata tttttccagc gtgaaatgcc cagcgaactg gtaaagacgg
    22021 ggaacccgtg ttcatgcgcg tattgcgccg ccttttcaaa acgcatatcg aaacacatgg
    22081 tgcagcggat gccgcgttcc ggctcaaact ccatgccttt ggctttggca aaccattctt
    22141 tgcggtcgtt ttcgtagtcg tcgtctttat cgatgaaagg aatgccgaac ttttccgcaa
    22201 agcgcacgtt ttcctctttt cggagcatat actctttgtg cggatggata ttgggattgt
    22261 aaaaataaat ggtgtagtcg atgccggagg caagcatcgc ctccatcact tcgccgctgc
    22321 acggggcgca gcaggaatgc agcaagactt ttttatgtcc gccgggcggg acgagtatgg
    22381 ggcggtcaat gtcggtaacg gtaggtttgt tttgtgtttc cattaactta acgatatctg
    22441 tcgaatacgg ttgaagcggc tattttatca gagccgccgc atgtaaaaca gggtgtcagg
    22501 gcatcagccc gagctgcctg aacgaacgga cggtttgcgc cgttacgata aaatggtcga
    22561 gcagcgacac atcgaccagc gacattgcct gtgccagccg ccttgtgaac atgatgtctt
    22621 cctgcgaagg ttcgggcgag ccgcccggat ggttgtgcgc gataatcagg ctgtcggcat
    22681 attcgtccaa tgccagtttg acaatttcac ggatgtaaat cgtgttttcc gcaaccgtac
    22741 cgcgcgacag ctctctgacc gcaatcagtt ggttttggcg gttcagcagc agcgcgacgc
    22801 tgacttcgac tttttcctgc cccaaatgaa agcgtaaata atcggccacc gtatccggat
    22861 cggacaggac gatgctctcc tgcaattctt ccgccaatat ccgccgcccg atttccctga
    22921 ccacggcaaa ctgtgtgaaa cttgccgtcc ccatcccttt gtatgccgac agtttgccga
    22981 cctccgcgct catcagcctc cccaaactgc cgaactcctg cagcaaataa cgcgccaaat
    23041 cgaccgcact cattccgcgt gtgccgacgc gcagcaggat tgccaaaagt tcggcatcac
    23101 tcaacgccgc cgccccgcgt tccaacagct tttccctggg tctttcgcct tccggccatt
    23161 gcttgatgct catgttttcc cttttttaaa tagtctgaga tgccgtctga aacagatgtt
    23221 cccacacgcc aaatatccga aatgccgtct gaaccggatt tccgcttcag acggcatctg
    23281 tatttcaaaa cagtcttacg ccgtttcttc caactcgatt gaagccgcca gaagcgacag
    23341 gcgtgccatt acgccgtaaa cgtagaggcg gttgtgttcg ttgtccacgt cgcagtcttt
    23401 ttcagagcgc ggcataccca gcgagtacca ttgttcgaat acgcgcttgc aggcttcggg
    23461 cgcttcttgg gaattatcgc cgttaccggt aggaatgctg ttagacagcg gaacaaacac
    23521 cataccgccg gcgttgaggt tttcgtccgc accgcgccct tcgtgtacgc ggaaaaagcc
    23581 gccgatgacg aagcggtcca tcatatacac gacgggttcg cacaccgcgc cgtttaaggt
    23641 ttcataagta taaatccctt cttggacaat cacttcgctg acttccaagc cttctttgac
    23701 tttcgccatt ttattgcggt ttttacggtt caatccgcgc acttcgtcgg cggatttgac
    23761 gctcatcacg cccatgccgt aagtgccggc atcggctttg acgatgacga aaggtttgtc
    23821 ggtaatgccc gattcgtcgt atttggcttg aattttcgcc agcacacgtt ctaccgcttc
    23881 cgccaacgcg tcttcgcctt cacgcccttg gaagtccaaa ccgccgattt tttcaaaata
    23941 agggttaatt tgccattcgt cgatgccgat taacttggca aattcggcgg taacttggtt
    24001 gtacgcgccg aaatgatttg ttttgcggcg cgtcgtccaa ccgccgtgca acggcggcaa
    24061 aacggtttgg ctgatgcctt tgaggatgtc gggaatgccg gcagacaaat cgttgttcaa
    24121 caaaaccacg caaggcgaaa agccgtctgc aagatggacg cggtcgcggg tacgcaataa
    24181 aggttccaac aggattttgt cgcccaatgc agtttcaaat tcggtcggtt cggttacttc
    24241 cggattcagg ctgcccaagc gcacttcata ccctgccgaa cgcaaaatct cgccgagggc
    24301 gtaaatgttt tgcaggtaaa acgtattgcg cgtgtggttt tcgggaataa tcaatacgga
    24361 tttcgccgtt tcgcaggcgc gttgcaccgc atcttgcgcg gcaaccgccg ccagcgggat
    24421 aaagttggga ttcagattat tgaagccgcc ggggaacaaa ttcatatcga tagacgaaat
    24481 tttgtaaccg gcattgcgta tatcgaccga accgtaaaac ggcgggcggt gtacgctcca
    24541 ctgcgtgcgg aaccacgcct cgattttggc gtgattggac aggatttttg cctcaaacgc
    24601 ctgaagttgc gccgaatgtt cgggcgacat caccggtaat ttcatctttc gtctgcctct
    24661 gtgtgttttt tgacaagatg cagatgataa tgcctttgaa agaatgacga caagtattct
    24721 ttcgtatcga atcatgcttt agatataact gtttactgaa atggacaata cgtctaattt
    24781 tttcgttaaa attgtttgca aaataacacc ggctcaaaaa ttagacaaaa tttgttgcgc
    24841 ggtataaaga atacgtctaa aatccgccca atccgcatta ccatttatcc aaaagaacaa
    24901 catcatgaca gcacaaaccc tctacgacaa actttggaac agccacgtcg tccgcgaaga
    24961 agaagacggc accgtcctgc tctacatcga ccgccatttg gtgcacgaag ttaccagccc
    25021 tcaggcattt gaaggcttga aaatggcggg gcgcaagctg tggcgcatcg acagcgtcgt
    25081 ctccaccgcc gaccacaaca ccccgaccgg cgattgggac aaaggcatcc aagacccgat
    25141 ttccaagctg caagtcgata ctttggacaa aaacattaaa gagtttggcg cactcgccta
    25201 ttttccgttt atggacaaag gtcagggcat cgtacacgtt atgggccccg aacaaggcgc
    25261 gaccctgccc ggtatgaccg tcgtctgcgg cgactcgcac acttccaccc acggcgcatt
    25321 cggcgcactg gcgcacggca tcggcacttc cgaagtcgag cacacgatgg cgacccagtg
    25381 cattaccgcg aaaaaatcca aatccatgct gattgccgtt gacggcaaat taaaagcggg
    25441 cgttaccgcc aaagacgtgg cactttacat catcgggcaa atcggcacgg cgggcggtac
    25501 aggctacgcc gtcgagtttg gcggcgaagc catccgcagc ctttctatgg aaggccgcat
    25561 gactttatgc aatatggcga tcgaagccgg tgcgcgctca ggcatggttg ccgtcgacca
    25621 aaccaccatc gactatgtaa aagataaacc cttcgcaccc gaaggcgaag cgtgggacaa
    25681 agccgtcgag tactggcgca cgctggtgtc tgacgaaggt gcggtattcg acaaagaata
    25741 ccgtttcaac gccgaagaca tcgaaccgca agtcacttgg ggtacctcgc ctgaaatggt
    25801 tttagacatc agcagcaaag tgccgaatcc tgccgaagaa accgatccgg tcaaacgcag
    25861 cggtatggaa cgcgcccttg aatacatggg cttggaagcc ggtacgccat taaacgaaat
    25921 ccccgtcgac atcgtattca tcggctcttg caccaacagc cgcatcgaag acttgcgcga
    25981 agccgccgcc atcgccaaag accgcaaaaa agccgccaac gtacagcgcg tgttaatcgt
    26041 ccccggctcc ggtttggtta aagaacaagc cgaaaaagaa ggcttggaca aaattttcat
    26101 tgaagccggt tttgaatggc gcgaaccggg ctgttcaatg tgtctcgcca tgaacgccga
    26161 ccgcctcgca ccggggcaac gctgcgcctc cacctccaac cgcaattttg aaggccgtca
    26221 aggcaacggc ggacgtaccc acctcgtcag ccctgccatg gcagccgcag ccgcagtgac
    26281 cggtcgcttt accgacatcc gcatgatggc gtaagtattt tcaggcggtc tttaagaatt
    26341 tgacgcggcc gtctgaaata tgtcccgtaa ggcagaaaca cctttaatat tcaaaccaag
    26401 gagtttaacc atgaacaaac ttttcattac cgccctgtcc gccctcgcct tgtccgcctg
    26461 cgccggcact tgggagggcg cgaaacaaga caccgcccgc aaccttgaca aaacacaggc
    26521 cgccgccgaa cgcgccgccg aacaaacagg caacgccgtc gaaaaaggct gggacaaaac
    26581 caaagaagcc gtcaaaaaag gcggcaatgc cgtcggacgc ggcatttccc atctcggcgg
    26641 aaaaatcgaa aacgccaccg aataaccgat tcgcgatgcc gtctgaaacc gcttcagacg
    26701 gcatcgtcag cacaagacgc aaacccatga aagcctttac caaaattacc gccatcgtcg
    26761 ccccgctcga ccgcagcaac gtcgataccg acgccatcat ccccaaacaa tttttaaaat
    26821 ccatcaaacg cagcggcttc ggccccaatg cctttgacga atggcgttac ctcgaccacg
    26881 gcgaaccggg catggacaac agcaaacgcc cgttgaaccc tgatttctcg ctgaaccagc
    26941 cgcgttacca aggcgcgcaa atcctgttga cgcgtaaaaa cttcggttgc ggctcttcac
    27001 gcgaacacgc cccttgggca ttggacgact acggcttccg cgccgtcatc gcccccagct
    27061 tcgccgacat cttctttaac aactgctaca aaaacggcct tttgcctatc gtgttgaccg
    27121 aagaacaggt tgaccggctt ttcaaagaag ttgaagccaa cgaaggctac cggctctcca
    27181 tcgaccttgc cgggcaaacc ctgaccaccc ccggcggcga aacattcgca ttcgacatta
    27241 ccgaacaccg caaacactgc ctcttaaacg gcttggacga aatcggactg accctgcaac
    27301 acgccgatga aattcacgcc tttgaagaaa aacgccgcca aagccagcct tggctgttta
    27361 acggttaaac ctatgccgtc tgaaggccgt tcagacggca ttcctgataa catataattg
    27421 ctgcaactaa tgtaaacaga ggggctataa attgctttca tctttcaagc gggcttagca
    27481 acgctctaaa ttaatgcaat acagctctat ttcaaataaa tttttagatt tttaatatgc
    27541 aacagattaa atttattgac ttattttccg ggatgagcgg tatcaggaaa ggatttgaac
    27601 aagcctgtcg gaaacagtcg gttgcttgcg aatgtgtttt tacctcagaa atcaaaccgg
    27661 cagctttgga agtattgaag caaaactacc ctgatgaagt gccgtatgga gatataacga
    27721 aaattgaaac gggagatatt cccgattttg acatcttgtt ggcaggcttc ccttgccagg
    27781 ctttttcttt cgccggaaaa agattgggct ttgaagatac gcggggaacg cttttctttg
    27841 atgtggcaag gattttaaag gcaaaaaaac caaaaggttt tattttggaa aatgtggaag
    27901 gattggtgac gcacgataga aaagatccga cacaaaaaat aggacgcacc ctgaccgtta
    27961 ttttggaaac cttggaaaca ttgggctact atgtttcttg gaaagtttta aatgcaaaag
    28021 atttcggcat tccccaaaac aggaagcgca tttatctgac aggcagtttg aaatccaaac
    28081 ccgacttgtc ttttgaaaca accctaagtc cgaaattaaa aaatattttg gaatcgggac
    28141 tgcctaccga aagcagtcct ttcatcaaaa aattgctaaa gaaatttccc ccgtccgaac
    28201 tgtacggaaa atcagtaaag gacaaacggg gagggaaaaa caatatccac agttgggata
    28261 ttgaattaaa aggcacagta accgaagaag agaagcaatt attaaatatg cttctaaaag
    28321 aacggaggaa gaaaaaatgg gcttcagaaa tcggcatcga ttggatggat gggatgcctt
    28381 tgacaaaagc gcaaatttca actttctata aacaccccaa tcttcaaaat attttggaca
    28441 gcctgacaga taaaggctat ttggttttgg agcatccgaa acaaaaaatt ggcgggcgaa
    28501 gaatcaaaga tgaatccctg cccaaaggct acaatattgt ttcaggaaaa aaatcatttg
    28561 aaatcaataa aatattagac ccaaatgatg ttgcgccaac cttagttgca atggatatgg
    28621 agcacctttt cgtcgttgac aacggcggtt tgcgcacact taccggaaaa gaaggtttac
    28681 gcttattcgg ctatccggac gattattcgt ttgatattcc taaaaaagac aaatacgatt
    28741 tattgggcaa taccgttgcc gttcctgtga ttaaggcggt atctgaaagg cttctgcata
    28801 ctttataaac cgatagggat aaaatggtaa aacttactgc acaacaaata tttgataagt
    28861 tgctgaatga ggaaaaaatc ttatcggtca atggtcaaat tagatttttc ttgggcgatg
    28921 tggatattat cgtcaaacaa aaagatgtcg tcggcaacat cattcaagaa tggctcggcg
    28981 gatggttgag aaaaagggaa attgaatttg atgtttcaac caatacccaa atgccccccg
    29041 atttcttttt aaataaaaaa gaccgcagta gagaattgtt ggaagtaaag gcgttcaacc
    29101 gaaatgccgg cccgggtttt gatattgcag attttaaaat gtattctgat gaaatcattc
    29161 ataaacccta tatgctggat gtagattatt taatattcgg ttacgatatg gacgacaacg
    29221 gcaatgtaac catcaaggat ttatggctta aaaaagtatg gcaaattacg agaagcatgg
    29281 atgggtgggc aattaatctt caagtcaaaa aaggcgtggt gcataaaatc cgcccgggtg
    29341 tttggtacag cataaataaa aagaatatgc ctatgtttga atgcttggaa gattttgttt
    29401 ccgcaattga agaaaccgtt tatcaaaacc cggcaacacg gcataacgca tccttatgga
    29461 aaaagaaatt tgaggaagcc tataaaaaac actacaaccg atcgatttcc atacctcgtt
    29521 ggcacgaaat tgcacacaaa tacaaaaaga aataaccctt caaccatcaa cccataagga
    29581 aacttgaaat gaccaaacat atcgccatcc tccgaggtga cggcatcggt cccgaaatcg
    29641 tcgccgaaac cgtccgcgta ctcgacaaac tcatcgccca aggcttggac atcagctacg
    29701 aatacgcccc attgggtggt gaagcctacg acgaatacgg ccatccttat cccgaattta
    29761 cgcaaaacct ctgccgcaaa gccgatgccg tcctgcttgg tgcagtcggt tctcctcaat
    29821 acgacaacct cgaccgtccg ttgcgccctg agcgcggcct gttggcaatc cgtaaggatt
    29881 tgaacctgtt tgccaatttg cgtcctgctg ttttatatcc cgaattagcc aacgcctcca
    29941 cgctgaagcc tgaaatcgtt gccggtttgg atattttgat tgtgcgcgaa ctcaccggcg
    30001 acatctattt cggcgaaccg cgcggcatcc gcgttctgga aaacggcgaa cgcgaaggct
    30061 acaacaccat gaaatacagc gaaagcgaaa tccgccgtat tgcccatgtt gccttccaat
    30121 ccgcccaaaa acgcagcaaa aaagtctgtt ccgtaggcaa agccaacgtt ttggaaacca
    30181 ccgaactgtg gcgcgaaatc tttgaagaaa tcggcaaaca atatcccgat gtcgaactct
    30241 cccatatgta tgtggacaac gccgctatgc agctcgtgcg cgcgcccaaa caatttgacg
    30301 tgattgccac cggcaacatc ttcggcgata ttttatcgga cgaggcctct atgctgaccg
    30361 gctccatcgg tatgctgcct tctgcttctt tggacgaaaa cggcaaaggc ctgtacgaac
    30421 cgtctcacgg ctccgctccc gacatcgccg gacaaaacaa agccaacccg ctggccacca
    30481 tcctctcgct cgcaatgttg ctgcgttaca gcctgaacga cgaagcgcgc gcgcaacaag
    30541 ttgaaaacgc cgtccaaaaa gtgctgcaac aaggcttgcg taccagcgac atttacgaag
    30601 aaggcacaaa actcgtttcc tgctccgaaa tgggcgatgc gatactcgcc gccttgtaaa
    30661 aaaacaaagc gcggatcaca ccgcgctttt tcatctgccc ttttccgccc gcaccgttcg
    30721 tcaaccgcct gcaaacccac cgtttaccgg caggaaatgc cataaatccc cgcctatgtt
    30781 aggataaaca cctttatcgg cccacacaaa ggattatcat catgaaaaaa atcatcttcg
    30841 ccgcactcgc agcggcagcc gttggcactg cctccgccgc cacctacaaa gtggacgaat
    30901 atcacgccaa cgcccgtttc gccatcgacc atttcaacac cagcaccaac gtcggcggtt
    30961 tttacggtct gaccggttcc gtcgagttcg accaagcaaa acgcgacggt aaaatcgaca
    31021 tcaccatccc cgttgccaac ctgcaaagcg gttcgcaaca ctttaccgac cacctgaaat
    31081 cagccgacat cttcgatgcc gcccaatatc cggacatccg ctttgtttcc accaaattca
    31141 acttcaacgg caaaaaactg gtttccgttg acggcaacct gaccatgcac ggcaaaaccg
    31201 cccccgtcaa actcaaagcc gaaaaattca actgctacca aagcccgatg gcgaaaaccg
    31261 aagtttgcgg cggcgacttc agcaccacca tcgaccgcac caaatggggc gtggactacc
    31321 tcgttaacgt tggtatgacc aaaagcgtcc gcatcgacat ccaaatcgag gcagccaaac
    31381 aataaatctt gatgcgaatc agcacaaatg ccgtcttcat caaaccgccc cgccaacggt
    31441 aagccgatat ggcggagcac aatccgccga aagatatttc agcggcaggc agggcttgcc
    31501 ggaaagtccg gaaagtatag tggattaaca aaaaccagta cggcgttacc tcgccttagc
    31561 tcaaagagaa cgatt
//
LOCUS       DAXIHS010000015        31233 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXIHS010000015 DAXIHS010000000
VERSION     DAXIHS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.015"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..278)
                     /locus_tag="ACFOF2_000590"
     CDS             complement(<1..278)
                     /locus_tag="ACFOF2_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC7106952.1"
                     /translation="MITVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPI"
     gene            complement(275..1045)
                     /locus_tag="ACFOF2_000591"
     CDS             complement(275..1045)
                     /locus_tag="ACFOF2_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC7106953.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDEASGKTGYKPVTTRYGNPYQ
                     ETVYIEISDGIGNNQTLISNSIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGSSSAKGKSGSERNTAKRGT
                     FRQETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNR
                     QHSSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            complement(1260..1619)
                     /locus_tag="ACFOF2_000592"
     CDS             complement(1260..1619)
                     /locus_tag="ACFOF2_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106954.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVHWKINNGNLIQTTN"
     gene            complement(1840..2295)
                     /locus_tag="ACFOF2_000593"
     CDS             complement(1840..2295)
                     /locus_tag="ACFOF2_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106955.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILGTFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            complement(2276..2905)
                     /locus_tag="ACFOF2_000594"
     CDS             complement(2276..2905)
                     /locus_tag="ACFOF2_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC7106956.1"
                     /translation="MVKTADGYKAIARIRTGDRVFSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKGSQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMPKNERLKIEKTIKNITKNANRKAKGEEHGRRGR"
     gene            complement(3039..3356)
                     /locus_tag="ACFOF2_000595"
     CDS             complement(3039..3356)
                     /locus_tag="ACFOF2_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106957.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="ACFOF2_000596"
     CDS             complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="ACFOF2_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC7106958.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVETLVNVLPFAKVKNLT
                     KAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGK
                     HISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKS
                     GLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="ACFOF2_000597"
     CDS             complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="ACFOF2_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC7106959.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            complement(5691..6791)
                     /locus_tag="ACFOF2_000598"
     CDS             complement(5691..6791)
                     /locus_tag="ACFOF2_000598"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC7106960.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRAVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAANARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            7116..8690
                     /locus_tag="ACFOF2_000599"
     CDS             7116..8690
                     /locus_tag="ACFOF2_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC7106961.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="ACFOF2_000600"
     CDS             complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="ACFOF2_000600"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC7106962.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            10669..11403
                     /gene="fnr"
                     /locus_tag="ACFOF2_000601"
     CDS             10669..11403
                     /gene="fnr"
                     /locus_tag="ACFOF2_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC7106963.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(11516..12466)
                     /locus_tag="ACFOF2_000602"
     CDS             complement(11516..12466)
                     /locus_tag="ACFOF2_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC7106964.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            12830..13558
                     /gene="rmpM"
                     /locus_tag="ACFOF2_000603"
     CDS             12830..13558
                     /gene="rmpM"
                     /locus_tag="ACFOF2_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC7106965.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            14409..15365
                     /gene="thiL"
                     /locus_tag="ACFOF2_000604"
     CDS             14409..15365
                     /gene="thiL"
                     /locus_tag="ACFOF2_000604"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC7106966.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            15358..15843
                     /locus_tag="ACFOF2_000605"
     CDS             15358..15843
                     /locus_tag="ACFOF2_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC7106967.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(15903..17579)
                     /gene="ettA"
                     /locus_tag="ACFOF2_000606"
     CDS             complement(15903..17579)
                     /gene="ettA"
                     /locus_tag="ACFOF2_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC7106968.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGCFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            17966..19321
                     /locus_tag="ACFOF2_000607"
     CDS             17966..19321
                     /locus_tag="ACFOF2_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC7106969.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            19325..20335
                     /locus_tag="ACFOF2_000608"
     CDS             19325..20335
                     /locus_tag="ACFOF2_000608"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC7106970.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
     gene            20569..22827
                     /locus_tag="ACFOF2_000609"
     CDS             20569..22827
                     /locus_tag="ACFOF2_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC7106971.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVVFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPYDEELGVFVQHDGFLDKGIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
     gene            22840..23505
                     /gene="pgmB"
                     /locus_tag="ACFOF2_000610"
     CDS             22840..23505
                     /gene="pgmB"
                     /locus_tag="ACFOF2_000610"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC7106972.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYLEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(23683..25191)
                     /gene="nadB"
                     /locus_tag="ACFOF2_000611"
     CDS             complement(23683..25191)
                     /gene="nadB"
                     /locus_tag="ACFOF2_000611"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC7106973.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(25397..26509)
                     /gene="nadA"
                     /locus_tag="ACFOF2_000612"
     CDS             complement(25397..26509)
                     /gene="nadA"
                     /locus_tag="ACFOF2_000612"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC7106974.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            26816..27754
                     /locus_tag="ACFOF2_000613"
     CDS             26816..27754
                     /locus_tag="ACFOF2_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106975.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            27830..28711
                     /gene="nadC"
                     /locus_tag="ACFOF2_000614"
     CDS             27830..28711
                     /gene="nadC"
                     /locus_tag="ACFOF2_000614"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC7106976.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            28739..29017
                     /locus_tag="ACFOF2_000615"
     CDS             28739..29017
                     /locus_tag="ACFOF2_000615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106977.1"
                     /translation="MSGKEPLNPNPVIASGRKCRLKDSSDGIFRKGRDALEKTAFQAG
                     ILFARQRGGIGRAGKRTGGGSFMPSESPPLRLFAKKWEKEHTILYNHP"
     gene            29117..29398
                     /locus_tag="ACFOF2_000616"
     CDS             29117..29398
                     /locus_tag="ACFOF2_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC7106978.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            29458..30228
                     /gene="xth"
                     /locus_tag="ACFOF2_000617"
     CDS             29458..30228
                     /gene="xth"
                     /locus_tag="ACFOF2_000617"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC7106979.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
BASE COUNT         6973 a         7869 c         8419 g         7972 t
ORIGIN      
        1 aaaataggtt gtctattgtc aatcaaatat tcagccactc ctgatagaaa agctgcaata
       61 ttttctttat tccattcttc agaacaggta aaaattaatt caaacctact tttatagttc
      121 aagtcatatc gattcaagcc taaagtagaa aaagtagtta cccctttata tggttgtgat
      181 gggaagacgg aaacgctgag atgataacgt cgatccaagc attttatacc gctttcgatt
      241 tcacccaaat aatgctctaa atgttcgact gtaatcattt tttcttaaac ctttcatcct
      301 tgttgccacc agatcgattg caatgtctgc attctaaact tactccttta ttataatcat
      361 ttagttgctc cttacgatca gaactatgtt gacggttact ccatgatgga ttatgacttc
      421 catcccaacc atcttttgta ttacgtttct ccttgttatg gggattacca tatacatctt
      481 taccacatgt aggacatttt tttgaattag gctggctacc atctgctgcc aaatcccatg
      541 cattttttag cgtttcttgt ctgaaggttc cacgtttagc agtatttctc tcactgccac
      601 ttttaccttt tgcgctagaa ctaccatacg gacaatcatt atgaacccaa accccttccg
      661 tttccgccct atcacccttg acgaagtaag tatgccaatc ggcaacggtc agattgtagg
      721 ctttgagcgg ctgctgtttg agggtaatgt tttgaaccgt ctgttttgca ccgctttcgg
      781 aaagcagggt gtcgcctttt ttcagacgac ctgcctgtat ccattttcct tgactgtaaa
      841 acggatggat gctgttggaa atcagggttt ggttgttgcc gatgccgtct gaaatttcaa
      901 tgtaaacggt ttcctggtac ggattgccgt atcgggtggt aacgggtttg tatcctgttt
      961 ttccgcttgc ctcgtccttg gcaaagacgc ggtcgccgac tcggatacgg gcaatggctt
     1021 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     1081 aaagaactta gttctcaata tcctgtttca ttcatcaaaa tgccgtctga aagctgaata
     1141 ccgcttcaga cggcattttg gtggttgggt ttttaagcca acctacgctt actgaaaacc
     1201 aaattgagtt tcagacagtt tttaggtttg ggtgtccaat ctaacttata tttgtccatt
     1261 taattagtcg tttgtatcaa atttccatta tttattttcc aatgtacttt ataactatct
     1321 tcataataat ctaattcaaa aaaacctgat atttcaatat ccaattccat tattgtttta
     1381 atgcattttt caaaataaat aatgaaataa gattttacgc atgcaccaaa aaaaatatag
     1441 ctgctccaat taaaactatt tgtcgggaaa acccacccgc ttttatatat ttttgcagat
     1501 tctttctctt cgatattgaa gggacaatta ttccaaaaat tattaatgtt ttcttggatg
     1561 atttttatat catcgtcaga gcattcaatc catcctttta tggaaacata tgatgccatg
     1621 tttaatctcc taaacctgtt ttaacaatgc cgccttttga ttcaatatat gacttaactt
     1681 gtgaatgaac accgtgcctg ttccgtacag caagcacagg ttggattaag attcgacaat
     1741 ctgtttggat tgacagaaag gtcgtctgaa aaatcgatta cggtttcaga cgaccttttg
     1801 ttgttgagtt ctcaacccaa cctatgcctg ccattaatgt tattcatcgt agtaaggttc
     1861 tgtcgaataa ttgtctttgc ccccggcaat gatagtaaca attttccctt ttgcttccca
     1921 agcttgtact cctatttcat caaactcata gacatatgtc ggataagatt catttgataa
     1981 ataatattta tcaacaccgt atgatttagg gtgatggaaa agctgtttaa aatcttcaaa
     2041 attcagacct attatattaa cgcccataaa atatagctcc tgataacaaa atatcgaaat
     2101 aattttgttt ttcttgacgg caattagtaa atttgagtcg ggagattcat aatattctgt
     2161 tctaaactca tcaggaggtt catacataaa agttcccagt atgtttttgt actgtgttat
     2221 atccgcacca aaacggaata ttcctacaga agtaaaaggt aaaaattcgg gagttttaac
     2281 gaccgcgtcg accatgctct tctccttttg cttttcgatt ggcatttttt gtaatatttt
     2341 tgattgtttt ctcaatcttt aagcgttcat ttttgggcat tccgggaata attttatttt
     2401 ttaattcagc aattcttgat tccgctgata tttgacttcg accgccgtct ccatgttttt
     2461 cattcttgga gcttcctgtt cttttaggcg gacaagaatt atgaacccaa accccttccg
     2521 tttccgcctg actgcccttg acgaagtaag tatgccagtc ggcaacggtc agattgtagg
     2581 ctttgagcgg tgttggtttg acaacgatgt tgcggacggt ttgggttttg ccactttcgg
     2641 ataacagcct gcttcccgct ttcaaatctt ccgctttaat ccatttgccg cccgaataaa
     2701 acggatggat gcggttggaa atcagggttt ggctgttgcc gatgccgtct gaaactttaa
     2761 tgtaaacggt ttcttgatac ggattgccgt atcgggcggt aacgggtttg tatcccgttt
     2821 ttccgcttgc ctcgtccttg gagaagacgc ggtcgccggt tcggatacgg gcaatggctt
     2881 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     2941 gaagaactca gttctcaata tcctatttca aatgaaggaa atgccgtctg aaaattaaac
     3001 acggtttcag aagacattgt gattgttggt tttttaacct acgcttgcga aattaattcc
     3061 tttctcctga atactgcacc atttatttgg tttaactcca tagaagaatg attagaataa
     3121 atacattcaa agtcagcttc atcaaaatat tctccacaca tagaccataa ctctgtaatc
     3181 aaatattcca tatcttcatg gtcttctgtg atggcttctc ttgataaata atttttgatt
     3241 tttaaaaacc ttcttccttc ctcgtaatct acggtaatta acctgatatc tgagtgtatc
     3301 atgcccagca ttgctatatt cataccgact cgcagccagc ttggtaatat attcataact
     3361 attgcacctt tttaatggtt ttagggttag ccggaacaat gtgaactcca tttttacctg
     3421 aatgaatcgt accaaaattg gtacttaatc ctgatttccc atcgctgcct ataggatacc
     3481 caaaatcaac aacaggattt cctcttgctc ctttagaaat aactggataa gcacctgaat
     3541 gtattccgtt caacaattct tgaggattaa tgttttgatt taaagtactc ttaccttcaa
     3601 tgtagtttct atgtcctgaa atatgtttcc cttgagctcc atcatgaatt ttagtcccaa
     3661 tagattttgc tttaaaatca acattgcggg tttgaaacat ttctccatct acagactgag
     3721 ataatcttga agcagtgtta tgcttgtagg agtctgagaa atccccacta accgcagcct
     3781 tccccggttt tgccgccttt gtcaggtttt tgactttggc aaacggcagg acgttgacca
     3841 gggtttcgac ggtttcggcg gcattggggt tttcctgcat ccactggtca acggcttcgc
     3901 ccgcgctgat aaaggggtta agcgcgccgg cggcgacgcc gttgataaac tccatgctgt
     3961 tgccccagcg gtcgagcttg gcattgtgct cgaacatttt tctgttggct tcatcggcgc
     4021 ggtcggagaa attgctgccg aggttgttgt agttgtcgga tatgcgttgc cggatgctgc
     4081 gggtgtcggt cggattgagt ttgatgctgc gtgctgtgcc gttgacgtga taggtgtatt
     4141 cgtcgcgtgc gcccgtaggt ttggggtaat tgccgccctt cgggccgtcg taggcatcgg
     4201 cgggatggtg ctcgtgtcct tcccagttga gccggtacac ggtaaagcct tcgtcaacgt
     4261 tgccttgttc tttgcttgcg ctgtcggcgg cgtggttgtc gaagggggcg tgttcttcgt
     4321 gtccgtgtcc ggaaaagcgg gtgtggtagc cgattgtgcc gttgatgttt gcctgttgga
     4381 tgagcaggtt gcccatctgg tgggtatagt cttggatgac gttgattttg ccggtgcggt
     4441 cggaaacgct gccgcgcggg tcgccgaaga ggtggtattt gccgccgggt tcgtagtgct
     4501 gccgttgggc gttatcggta atgaacgggt cttgcgccaa gtccgccgcg agggcgggct
     4561 gtccgaaggc ggcgaccgct acggcgcagg cggcaaggag gtttgtcagt ctgcgcagcg
     4621 gtttcacggt ttatcctcct ttgcggcggc ggatgacttc gttgccgaca tcggggtttt
     4681 taccgttgtt ttgtttgaag tcgggacggt tttgggcggt tgtgtcgccg tagggggtga
     4741 tgtcggagaa atcgaccatc aggcggtctg aggctttgac ggttttgctg actttgtaag
     4801 ggccggtcca aagggcgtat tgttcttggt attgggattc gtaggcggcg gttttagggg
     4861 caatcagcag tttccggctg tcgcggtcga cggcgaaata ttcgagcttg gtttgggctt
     4921 taagggtttc ggcgttgtag aggtgcagtt cggtacggct gcggacggtg ccgaatacgt
     4981 cgacggttac gaatacgtcg gtgtcggcgt attcgggcgg tacgacttcg atgccgcgca
     5041 ggtagaagac ggtttggatg aggttggtca ggaaggaaac gtcgcggggg ttggcgagca
     5101 gggtttcgtt gcggtagtcg cccatgccgt tgacggacag tccggcggag cgttcgcctt
     5161 tgcgtccgct gtttctagtc agggcggcgg cgggggcgtt caaaagcgat gtggaagtgg
     5221 ttacgctgga gagcgcgtcg gatttggtgg tggcggtagt gtcgtaggcg gggtagctgt
     5281 attgggtggc actttcgggg ttgttgtggt agccaccgcg tatcagtgcg tcgatagagt
     5341 agcgtccgcc gcttatgttg cccgaacctt ggtcgcccat aacggagacg taaagggcgg
     5401 ctttgcgtcc tttcagggcg gacaaatcca tttctttgac ggcggcgcgg gacgatgcgg
     5461 cgacgagttc ttgttcgacg gcaaagcgtt tgccgccgcc gtgggcgggt atgccggtca
     5521 gtgtgccgca ggcggttagg acgaggggga tgaggaggag cagggttttc atggcagggt
     5581 ttgttttgtt ttgaacgagt ttgagtgtaa atggatttta agggtttgta aacgaaagag
     5641 gaaaaaatgc cgtctgagcg gcggaaatgg ctttcagacg gtatttgcgc tcaataataa
     5701 tatcccgcgc ccagaataca cggtttggat gcgccggttg ctttgtgcgg actaccggga
     5761 atgcgattaa tccaacacgc cgccaaccac gcaaatgcgg cggcttccac ccattgcgga
     5821 tcgaggttca ggtcggcggt gctgtgcagg gaaacgcgtg tgccgaaaca ttccgccaaa
     5881 tccgccatta aaacaggatt gcggataccg ccgccgcaaa tgtacatttg acgggcattt
     5941 gccgctgcgt gtgagacggc gtcgaaaacg gtttgcgcgg taaaacggga aagcgtccgc
     6001 aatacgtcgt atcggttttc gccgccgcca aggtaggttt cgagccaatt tagggcaaac
     6061 agttcgcgcc ccgtgctttt ggggtggggt cgtgcgaaat acgggtgggc gagcagcctg
     6121 tcgagcagtt gcggcaatat gttgccttgt gccgcctttg caccgttttt gtcgtaagga
     6181 agctgccagt gtgcctgcgt ccacgcgtcc atcagcatat tgcccggccc tgtgtcgaag
     6241 ccgaaggcgg gtgcgtcgga ggggagtacg ctgatgttgg caatcccgcc gatgttcagt
     6301 accgcgcgtg tttctttgtc atcgcggaac agggctttgt gaaaggcggg gacgagcggc
     6361 gcaccttgtc cgccggccgc aaggtcgcgg ctgcggaagt cgccgacggt aaaaatctga
     6421 gtcagttccg ccagcagcgg caaatcggca agctgtatgc tgtaaccgtg ttccggtgca
     6481 tgtcggacgg tttgcccgtg gcagccgagg gcggtaatgt cggacggcgc gaggttttga
     6541 ctgcacagca gttcggcggc ggtttgcgca tataggcggc tgagttcttg cgacaaaatc
     6601 ctgctgcggt gcagttcgtc tgtgcctgtg tcctgcaaat ccagcaattg gcggcgcagc
     6661 ctgtcgggat agggggtaaa ggcgtgccct tccgcgccca gccatttgcc gccgtccatc
     6721 cgtaccagca cggcatccgc cccatccata ctggttcccg acatgatgcc gatgtaaagc
     6781 tgtgtttcca tcatcactcc caaactggtg caaaacgcca ttttaacgtg tattgacgct
     6841 cgtataccga tttgccgccg cagtgtaaat aaagtgtaaa taaatgtttc aagacggatg
     6901 gaaaaatatt ataatgcgcc cgcaacatcc agtagtagaa gtgtcataca aaccgtttcc
     6961 ggcagcagtt ttgcattcgg tcaggtttgg gggtattcgg atgcggttca gaaggaagga
     7021 tgcgtctgcc atatcccgaa acggcagttc gaccggaggc agcagtacag tgtcggcaac
     7081 actcatgatt tccaccacat taaaggaaga ttgccatggc tcaaatccaa atgagcgcaa
     7141 atgttaaaac catcaacgcc gtctttgccg ccatgctggt aggtacagtc ggctatttta
     7201 tttattgggg cttgggttat acccattaca attacgccgc cttattcatt attgccacga
     7261 tgttcggcgt gtttatggcg ttcaacatcg gcggcaacga tgttgccaat tctttcggca
     7321 ccagcgtcgg tgcgggtacg ctgaccatcc cgcaggcttt gctgattgcg gcggtatttg
     7381 aggtcagcgg cgcggtcatc gcgggcggcg aggtaaccaa taccatacgc aaaggcatcg
     7441 tcgatttgaa gggtgttgat ttcgaaccca tacagtttgt gtttattatg atgtccgcgc
     7501 ttttggcggc ggcgttgtgg ctgttgtttg cctcgaaaaa agggcttccg gtatctacca
     7561 cccattccat tatcggcggc attgtcggca gcgcggtatg tatggcggta atgaacgatg
     7621 ccgcatcggg cgatttgata cgttggggca agctgggcgg tattggtgtt tcttgggtat
     7681 tgtcgcccgt gttgggcggc gcggtgtcct atttcctgtt ttcgcgcgtc aagaaaaacg
     7741 tcttagatta caacgcttgg gcggaaggca cgctcaaggg catcaagcag gaaaaaaagg
     7801 cctataaaga acggcaccgc ctgtttttcg agggtttgtc cgaagccgaa aaagtcgagt
     7861 acgccaccaa aatggcgcac gacgcgcaaa tttacgacga acccgaattc gatccgcaag
     7921 agctgcaatc ggagtattac cgcggtcttt atgcgttcga caaccgtaaa aacaatgtcg
     7981 attcctacaa agcgctgcat tcttggattc cctttatcgc ttcgttcggc gcgatgatga
     8041 tttccgctat gctgattttc aagggcttga aaaacctgca tttggggatg agcaacgtca
     8101 acagcttcct gaccatcttt atgataggcg cggcggtgtg gatggggacg tttgtgtttg
     8161 ccaaaagcct caagcgtaaa gacttgggca aatcgacctt tcagatgttt tcatggatgc
     8221 aggtctttac cgcctgcggc ttcgcattca gccacggtgc gaacgatatc gccaacgcca
     8281 tcggtccgtt tgccgcgatt atggatgttt tgcgtaccaa cagcgttgcc gcgcaaaatg
     8341 tcgtcccccc gattgcgatg ctgactttcg gcatcgcgct gattgtcggt ttgtggtttg
     8401 tcggtaaaga ggtgattaaa accgtcggta cgagtttggc cgaaatgcac cccgcctcgg
     8461 gttttaccgc cgaattgtcc gccgcatccg tcgtgatggg cgcgtcgctg atggggctgc
     8521 ccgtgtccag tacgcatatc ttggtcggcg cggtactcgg catcggtctg gtcaaccgca
     8581 atgccaactg gaaactgatg aagcccatcg gtttggcgtg ggtcattacc ctgcctgccg
     8641 ccgccgtatt gtcggttgtc tgctacttgg ttttacaggc agtattctga ttgtaaaata
     8701 ctgatgccgt ctgaacccgt gttcagacgg cattttgttg atggaatgtg cgggcttgtg
     8761 ccttatgcac aatctgttct gtcgggatat gccgtttggt atagtgatta acaaaaatca
     8821 ggacaaggcg acgaagccgc agacagtaca gctagtacgg caaggcgagg caacgctgta
     8881 ctggtttttg ttaatccact atatcttggt ttcggaacgg tcggacacaa aggtgcggaa
     8941 cgttatgata tgccgccgcc tgttcttgaa aacacttatc ctgccggcag caaaatgccg
     9001 tctgaaaaag cctttcagac ggcatttgta cgttagacgc aatcacaccg tttgcgaata
     9061 tttcgccttg gtttctttat ggcgcaggtg gtaatcgaac accatggcga tgttgcggat
     9121 gaggaagcgt cctttcgggg taacggtcag cccgtggctg ttcaggcgca ccaatcccaa
     9181 accggcgagt ttttccaaat ccgccagttc gtctttgaag tagcggtcga acgggatgcc
     9241 gaacatactt tcgtaaatcc ggtagtcgag cgcgaaacgg cacatcaaat cctgaatgat
     9301 gttgcggcgc aggatgtcgt cctgattgag ctgataaccg cgcatgatgg gcagtctgcc
     9361 ttcgtcgatg gcggcatagt aggcatcgat gtcgcgttcg ttttgggaat aggtgctgcc
     9421 gattttgccg atggacgaca cgccgatggc gaccaaatcg caatccgcgt aggtcgaata
     9481 gccttggaag ttgcgctgga ggaagccttc tttgagggcg atggagagtt cgtcgtcagg
     9541 tttggcgaaa tgatccatgc cgatgaagac gtagccgcgt tcggttaggg tttggacgca
     9601 gtattgcagc atatcgagct tctcttcgct gtcgggaacg gcggcggtat cgatgcggcg
     9661 ttgcggtttg aacacgtgcg gcaggtgggc gtagtgataa agggcgaggc ggtcgggatc
     9721 gagcgacaaa acggtatcga tggtggtttt gatgctttcc gaagtctggt gcggcaggcc
     9781 gtaaatcaaa tcgacgctga cggatttgaa ccccgcttcg cgcgccgcat cgatgacttc
     9841 tttggtttct tcgtaacttt ggatgcggtt gaccgccgcc tgcactttgg ggtcgaaatc
     9901 ctgaatgccg atgctcatgc ggttgaagcc gagtctgccg agcatgagga cggtgtcgcg
     9961 gctgactttg cgcgggtcga tttcgatgga gtattcgccg gtggggatta actcgaaatg
    10021 tttgcgtatc atgcggaaga cacgttcgat ctgttcgtcg ctcaaaaagg tcggcgtgcc
    10081 gccgccgaag tgcagttggg caagctggtg ccgtccgttc agatgtggag cgagcagttc
    10141 catttctttt tcaagatatt cgatgtaggc atcggcgcgg cttttgtctt tggtgatgat
    10201 tttgttgcag ccgcagtagt agcagatggt gttgcagaac ggaatgtgaa tgtaaaggga
    10261 aagcggtttg tttaacgcgc ccataccgcg caaatgtaaa gctttgatat attcgccttc
    10321 gcggaaaccg tcatggaaac ggtcggcggt agggtaggaa gtgtagcgcg ggccgctggc
    10381 gggcaggctg gcaatcagcg cgcggtcaaa ctcggggcgg tcatcgttta cattgtgatt
    10441 gttctgtatc tgaatgattt tcatggtgtg tgtgtgcgat tttatgatgt tagtcaaatt
    10501 ttggatagtt tggtagaatg ccacagtatg ataaacctgt cttgatatgt gtcaataagc
    10561 acatatagtg gattaaattt aaataaggac aaggcgaggc aacgccgtac tggtttaaat
    10621 ttaatccact ataatcatga tggggcaaag cgcacaaaaa ggtacggtat ggcttcgcat
    10681 aatactacac atcagatgaa aacgctgtgt tcttcctgtt ctttgcggga actctgcctg
    10741 cctgtcgggc tgctgcccaa cgagctcagc caactcgatg ccgtcatccg tcaaagccgc
    10801 cgcctgaaaa agggcgaata cctgttctgt gtcggcgaag cctttacctc gctctttgcc
    10861 atccgttcgg gcttcttcaa aacaaccgtc gccagtcagg acggccgcga tcaggtaacg
    10921 ggtttcttta tgtcgggcga actcatcggc atggacggca tctgttccca tgtgcacagt
    10981 tgcgacgcgg tcgccttgga agacagcgaa gtgtgcgaac tgccgtttac ccacatcgaa
    11041 gaactggggc aaaacatccc cagcctgcgt acgcacttct tccgcatgat gagccgtgaa
    11101 atcgtgcgcg accaaggtgt tatgctgctg ttgggcaata tgcgcgccga agagcggatt
    11161 gccgccttcc tgctgaacct ctcccaacgc ctttattccc gtggttttgc tgccaacgat
    11221 ttcatcctgc gcatgtctcg tgaagaaatc ggcagttatc tcgggctgaa acttgaaacc
    11281 gtcagccgca cattatccaa gttccatcag gaaggattga tttccgtcga gcataagcac
    11341 atcaaaatcc tcaatctgca ggtgttgaaa aaaatggtgt ccggctgctc gcacgccatt
    11401 tgattaaccc gtacgaacat ttcagacagc attctcaata aacacagggc agacgaaaac
    11461 atctgtcctg tttgttgtat ctgccgcaaa gtgccgtctg aaaaccggca gccgcctaaa
    11521 tcgaaaaatc ctcgctgatg ggcgtgtaca gaatcctatc caccttctcg cgtgtcaggt
    11581 gcggcgcaaa cgcttggata aagtcgtagg catatccgcg caaataagta tcgctgcgca
    11641 aagcaatcca cgtcggcgac ggctcgaaca ggtgtgccgc atccacaagc tgcaaatcgc
    11701 cgtccgtatc cgggttgtac gccattttcg ccatcagtcc cacgcccaaa cccaagcgca
    11761 cataagtctt caatacgtcc gtatccgccg ccgccaatgc gacatccggc tgttccaaac
    11821 gggctttgga aaatgcccgc gcgatgctgc tgcccgcatt gaatgcaaat tcataagtaa
    11881 tcagcggaaa cctcgccaaa tcttcaatgc ggagggggtt tctgcattcg agcaaggggt
    11941 ggtcgttcgg tacgataacc gcatgagtcc agtcatagca gggaagtttt cccagttcgg
    12001 gatggtcgtc tatccgttcc gtaacaatcg ccaagtccgc ctcgcctgag gtaaccatac
    12061 gtgcgatggc ggcagggctc ccctgtttga tggtcaggtt gactttcgga tagcgtttca
    12121 caaaatcggc aacaatcaag ggtagggcat agcgtgcctg agtatgcgtc gtggcaaccg
    12181 tcagcgaacc gctgtcctgt ccggtaaact cgctgccgat atttttaatg ttctgtacat
    12241 cgcgtaaaat acgttccgca atatccaaaa ccaccttgcc cggctgcgag accgaaacca
    12301 cgcgcttgcc gctgcggata aaaatctgaa tgccgatttc ttcttccagc aatttgattt
    12361 gtttggagat gccgggttgc gaagtaaaca aggcttcggc cgcttcggaa acgttcaggt
    12421 tgtgctggta aacttctaag gcgtatttca attgttgtaa tttcatggcg ggtcggtgtg
    12481 ggtctgtgtc gggtggctga acattgttta taatttatca tattttcttg ccggtacggt
    12541 atggggcttt gccgttgtgt ttgttgtttt tgtgcaacgg caatcgtgcg atatggaaaa
    12601 aatcccccta aagtaatgac acggaattga tttttcggca tgatagacta tcaggaaaca
    12661 ggctgtttta cggttgtttt caggcgttga gtattgacag tcctccccct gcttctttat
    12721 agtggagact gaaatatccg atttgccgcc atgtttctac agcggcctgt atgttggcaa
    12781 ttcagcagtt gcttctgtat ctgctgtaca aatttaatga gggaataaaa tgaccaaaca
    12841 gctgaaatta agcgcattat tcgttgcatt gctcgcttcc ggcactgctg ttgcgggcga
    12901 ggcgtccgtt cagggttaca ccgtaagcgg ccagtcgaac gaaatcgtac gcaacaacta
    12961 tggcgaatgc tggaaaaacg cctactttga taaagcaagc caaggtcgcg tagaatgcgg
    13021 cgatgcggtt gctgcccccg aacccgagcc agaacccgaa cccgcacccg cgcctgtcgt
    13081 cgttgtggag caggctccgc aatatgttga tgaaaccatt tccctgtctg ccaaaaccct
    13141 gttcggtttc gataaggatt cattgcgcgc cgaagctcaa gacaacctga aagtattggc
    13201 gcaacgcctg agtcgaacca atgtccaatc tgtccgcgtc gaaggccata ccgactttat
    13261 gggttctgac aaatacaatc aggccctgtc cgaacgccgc gcatacgtag tggcaaacaa
    13321 cctggtcagc aacggcgtac ctgtttctag aatttctgct gttggcttgg gcgaatctca
    13381 agcgcaaatg actcaagttt gtgaagccga agttgccaaa ctgggtgcga aagtctctaa
    13441 agccaaaaaa cgtgaggctc tgattgcatg tatcgaacct gaccgccgtg tggatgtgaa
    13501 aatccgcagc atcgtaaccc gtcaggttgt gccggcacac aatcatcacc aacactaagg
    13561 ctaggcaata tcttgccgat gcatgaggtt agtggatttt gtaccaggta ctgttgcaat
    13621 attcgtgaaa cgtcggtcgg catcgatgat gtgaaacaaa cccccgcttt tgcggggttt
    13681 gtttttttgg gtggttttct gaaacggcta tcgtcagaat cggggtgcag gttcggattc
    13741 ggattcggat tcggattcag attcagattc agattcaggt ttgtgtccca ttgccgcgct
    13801 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
    13861 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggtg
    13921 aggcaacgct gtactggttt aaatttaatc cactatatcg gttgaaactc tgattttaag
    13981 gcggtaggat gtgggtttgc ccatagaaag ggaatccttt ctgtatcaag ccctgaaagg
    14041 gataattcat acaaattcac gcctttcccc ctcattggga aatggatgga atcgtgccag
    14101 atgtgtgcgg cactgtatgc cggatatggt tttatcatca gcccttttcg gttgaaacct
    14161 cgccagttgc agcgattgag cctaatcggt ggcggaagtt gccgctttgc attcggggtg
    14221 gcgtgcagtg cggtgctttg atatgccgtt tgtgtgttga aacagggtgg tcggtgcata
    14281 cgggtacggt atggccaaag ctaaaagtga aatacgctga aacactgaat gagccgcttt
    14341 attgtttgta cggcctttgc tgccttgcta tgatttaaat tggattcgcc cgccggatat
    14401 tttgggatat gaaagaattt gacttcatca aacggtattt gcaaacaggc acggataatg
    14461 atgtcgtatt gggcataggc gacgatgcgg cgattgtccg cccgcgtgaa ggcttcgatt
    14521 tgtgtttcag tgcggatatg cttttgaagg acaggcattt ttttgcagat gtcaaacctg
    14581 aagacttggc ttggaaggtt ttggccgtca atatttcaga tatggcggcg atgggtgcga
    14641 taccgcgttg ggtgttgctg agcgcggctt tgcccgaatt ggatgaggta tggctgaaac
    14701 ggttttgcgg cagctttttc ggtttggcaa aaaagtttgg cgtaacgtta atcggcggcg
    14761 atacgaccaa gggcgatatg gcgttcaatg taaccattat cggcgaattg ccgaagggta
    14821 gggcgttgcg gcgtgatgcg gcggttgcgg gcgacgatat ttgggtgtcg gggcgtgtcg
    14881 gtatggcggc ggcggctttg aactgccgtc tgaaacggtg tgtgttgccg gatgatgtgt
    14941 ttgccgaatg cgaacaaaag ctgctccgcc ccgagccaag ggttgggctg gggcttgcgc
    15001 tgttgccgtt tgcccgggcg gcgcaggatg tttcagacgg catcgcgcaa gatttggggc
    15061 atatcctgac cgcttccggc gtgggggcgg aaatttgggc cgattcgctg ccgtctttat
    15121 ccgtattgaa agatattttg ccccgagcgc aatggctgtc ttatactttg gcgggcggcg
    15181 acgattacga gctggtgttt accgcgccgg aaagttgccg cagccgcgtg cttgatgcgg
    15241 cagaacggtg cggcgtgccg gtaacgcgca tcggcaaaat caacggagga tgccgtctga
    15301 aagttttaga tgacggcggc agggaattgg aactacattc tttaggattc gatcattttg
    15361 gctgatttta aacctgactt tgcgtggctg ttgaaacgac cgttgtgttt tttggctttt
    15421 ggtttcggca gcgggctggc tccgttcgcg ccgggtacat ttggcacttt ggcggcactg
    15481 cctttggcat ttgtgctgat tttgctcggc atagacgggc tgctgctggc ttttttgtgt
    15541 atcgtgctgt ttatgtgggg catacgcatt tgcgcttatg cggaacgtga aacgggtgtc
    15601 agcgaccatg gtgggattgt ttgggacgag attgtcgcca tgctgtttgt gctggcgttt
    15661 gtgccgttca ggtggacgtg gtggctggcg gcatttgtcc tattccgtct gtttgacgcg
    15721 ctcaaaccgt ctcccgtcgg ttggtttgac aagaatctgc acggcggttt gggcattatg
    15781 gcggacgata tggcggctgc ggtgatgact ttgattgtct tgaggattgc aatgctgttt
    15841 taaacggtgc tgccttgtaa aaatgccgcc tgaaagcctt tcagacggca ttgtttcgga
    15901 ggttaacgcg ttaccggttt gtatttgatg cgtttcggtt tcgcgccttc ttcgcccaaa
    15961 cggcgtttct tatcggcttc gtattcctga tagttgccgt cgaagaacac ccatttggag
    16021 tcgccttcgc aagccaagat atgcgtagca atacggtcga ggaaccaacg gtcgtgagaa
    16081 atcaccatca cgctgccggc aaattccaac aatgcgtctt ccagcgcgcg cagggtttcc
    16141 acatcgaggt cattagacgg ttcatccagc agcaatacat tgccgccgct caacaaggtt
    16201 ttcgccaagt gcaaacgtcc gcgttcgccg ccggaaagct gccccgtgat tttgctttgg
    16261 tcgctgcctt tgaaattgaa gcatcccaaa tattggcggg cggggatttc aaactgcccg
    16321 acctgtaaaa tatcgcgacc ttcggcaatg ttgtcgaaca cggttttgtc gttttgcaaa
    16381 ccttcgcggc tttggtcaat caagctcatt ttcacggttt gcccgatttt cacttcaccg
    16441 gaatcgggct gctctttgcc cgcaatcatt ttgaacagcg tagatttacc cgcgccgttc
    16501 ggaccgatga tgccgacaat cgcgcccgca ggcactttga agctcaaatc gtcaatcagc
    16561 actttatcgc cgaacgattt ggaaacattt acaaattcaa tcacttcgtt acccaaacgc
    16621 tcggcaacgg gaataaagat ttcctgcgtt tcattgcgtt tttggtattc gtagttgctc
    16681 atttcctcaa aacgcgccaa acgcgctttg gacttggctt ggcggccttt ggcattttgg
    16741 cgcacccatt ccaattcctg cttcatcgcc ttcacgcgcg cggcttcgga ttttgcctcg
    16801 ttttccaagc gtttttcttt ctgctccagc caagacgagt aattgccttt ccacggaata
    16861 ccatggccgc ggtcgagttc caaaatccat tcggcggcgt tgtcgaggaa gtagcggtcg
    16921 tgcgttaccg caacgactgt gccggggaag cgcacgagaa attgctccag ccactcgacc
    16981 gattccgcat ccaagtggtt ggtcggctcg tccagcaaaa gcatatcggg cttgctcaac
    17041 aagagtttgc acaaggcaac gcggcgtttt tcaccgccgg acaaattatc gattttggca
    17101 tcccattccg gcaggcgcag cgcgtcggca gcgatttcca attcatgctc tgcaccgccg
    17161 cccgtggacg aaccggccgc gataatcgct tccaagcggc cctgctcttc cgccaacgcg
    17221 tcaaaatccg catcgggatt ggcatactcg gcatacactt cttccaagcg tttctgcgcc
    17281 gccgccactt cgcccaaacc gctttccact tcctcgcgca cggttttttc aggatcaagc
    17341 tcaggctctt gcggcaggta gccgattttg atgccgccca tcggcacggc ttcgccctca
    17401 aattccttat ccacgcccgc cataatccgc agcacggtgg acttgcccgc gccgttcaaa
    17461 ccgagcaggc cgattttcgc gccggggaag aaagaaaggg aaatatcttt aataatggtt
    17521 ttctgcggcg gcacaacctt gctcacgcgc agcatagaat agacgtattg ttgggacatg
    17581 gttttctcgt tttcatcaaa caaatttcag acggccattt taaccgataa tttgatttaa
    17641 gccagtttat ccgcgaaccg gtattgccaa aatcgggcag gattcataaa atccgcttat
    17701 ccctttgaaa ttatatagac aaaaaaataa taatgatagg ggatcgccgc cccggcaacc
    17761 atttcggatt ttccaaagca aatatagtgg attaacaaaa atcaggacaa ggcgacgaag
    17821 ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
    17881 cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc tactatactt
    17941 ttcaaatcaa aaaaggattt accttatgtc ggaatatacg cctcaaacag caaaacaagg
    18001 tttgcccgcg ctggcaaaaa gcacgatttg gatgctcagt ttcggctttc tcggcgttca
    18061 gacggccttt accctgcaaa gctcgcaaat gagccgcatt tttcaaacgc taggcgcaga
    18121 cccgcacaat ttgggctggt ttttcatcct gccgccgctg gcggggatgc tggtgcagcc
    18181 gattgtcggc cattactccg accgcacttg gaagccgcgt ttgggcggcc gccgtctgcc
    18241 gtatctgctt tatggcacgc tgattgcggt tattgtgatg attttgatgc cgaactcggg
    18301 cagcttcggt ttcggctatg cgtcgctggc ggctttgtcg ttcggcgcgc tgatgattgc
    18361 gctgttggac gtgtcgtcaa atatggcgat gcagccgttt aagatgatgg tcggcgacat
    18421 ggtcaacgag gagcagaaag gctacgccta cgggattcaa agtttcttag cgaatacggg
    18481 cgcggtcgtg gcggcgattc tgccgtttgt gtttgcgtat atcggtttgg cgaacaccgc
    18541 cgagaaaggc gttgtgccgc agaccgtggt cgtggcgttt tatgtgggtg cggcgttgct
    18601 ggtgattacc agcgcgttca cgattttcaa agtgaaggaa tacgatccgg aaacctacgc
    18661 ccgttaccac ggcatcgatg tcgccgcgaa tcaggaaaaa gccaactggt tcgaactctt
    18721 aaaaaccgcg cctaaagcgt tttggacggt tactctggta cagtttttct gctggttcgc
    18781 cttccaatat atgtggactt actcggcagg cgcgattgcg gaaaacgtct ggcacaccac
    18841 cgatgcgtct tccgtaggtt atcaggaggc gggcaactgg tacggcgttt tggcggcggt
    18901 gcagtcggtt gcggcggtga tttgttcgtt tgtattggcg aaagtgccga ataaatacca
    18961 taaggcgggt tatttcggct gtttggcttt gggcgcgctc ggctttttct ccgttttctt
    19021 catcggcaac caatacgcgc tggtgttgtc ttatacctta atcggcatcg cttgggcggg
    19081 cattatcact tatccgctga cgattgtaac caacgccttg tcgggcaagc atatgggcac
    19141 ttacttgggc ctgtttaacg gctctatctg tatgccgcaa atcgtcgctt cgctgttgag
    19201 tttcgtgctt ttccctatgc tgggcggctt gcaggccact atgttcttgg tagggggcgt
    19261 cgtcctgctg ctgggcgcgt tttccgtgtt cctgattaaa gaaacacacg gcggggtttg
    19321 agcgatgagc gatacccccg ctacccgcga tttcggcctg atcgacgggc gtgccgtaac
    19381 cggctatgtg ctgtccaacc ggcgtggtac gcgtgtctgc gtgctggact tgggcgggat
    19441 tgtgcaggaa ttttccgttt tggcagacgg cgtgcgcgaa aacctcgtgg tgtcgttcga
    19501 cgatgcggct tcctatgcgg acaatccgtt tcagattaac aagcagatag ggcgcgtggc
    19561 cggacgcatc cgcggtgcgg cgttcgacat caacggcagg acttaccgcg tggaggccaa
    19621 cgaaggcagg aacgcgctgc acggcggttc gcacgggctg gccgttaccc gtttcaacgc
    19681 ggtggcggca gacggccgtt cggtggtgct gcgcagccgc ctgcaacagt cggccgacgg
    19741 ttatcccaac gatttggatt tggatatttc ctaccgcttg gacgaggacg accggcttac
    19801 cgttacctat cgcgccaccg cgctcggcga cacggtgttc gacccgacgc tgcacattta
    19861 ctggcggctg gacgcgggcc tgcacgatgc ggttctgcat attccgcagg gcggacatat
    19921 tccggccgat gccgaaaaac tgcccgtctc aacggtttca gacgacctcg aagtatttga
    19981 tttcagccgg cccaagccgc tggatgccgc cgttgccgcc ctgcgccgcg aaacgggtcg
    20041 ggccggtttt gacgacgctt accgcgtgcc gtccgatata ggccgtcccg ccgctgtgtt
    20101 gcaagccgga cgccgccgtc gtatcagcat atacagcgac cgcaacggct tggtcatctt
    20161 taccgccgcc ccgcaggatt tcgcgcggca cgatgcgggc gtttacgacg cgctggcgac
    20221 cgaggcgcag acgctgcccg acagcctgaa ttggcccgag ttcggcaata ttcgtctgaa
    20281 caagggtgat actagggagg cgacgattgc ttacggcatc gaatcccttt cttaggagct
    20341 tcctaacacc ggttgcagac ggccttttta tagtggatta acaaaaaccg gtacagcgtt
    20401 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtaaatcggt
    20461 tccgtactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
    20521 ataagatttc accattccct caaatcaatc caaacaggag cttcataaat gtacacaaga
    20581 attatggaaa tcagcccttg gacgctgcgt tcggcaaagc tggaaaaaga acacaaacgg
    20641 ctgcaagaga gcctgaccag tttgggcaac ggctatatgg gtatgcgcgg cagctttgag
    20701 gaaacctatt ccgccgacag ccacttgggt acctacatcg ccggtgtgtg gtttcccgac
    20761 aaaacccgcg tcggctggtg gaaaaacggc taccccaaat atttcggtaa agccatcaac
    20821 gcgctcaatt tcagcaaagt caaaatcttt gtcgacggac aagaggtgga cttggcgaaa
    20881 aacgatgttg ccggcttctc cgtcgaactc gatatgcagc acggcgtgtt gcgccgctcg
    20941 ttcaccgtat tcggtgtgcg tttcaatgtg tgcaaattcc tgtccgtcgc gcaaaaagag
    21001 ctggcggtta tccgctggga agccgtatcc gttgacggca aaacccacca agtccgcatc
    21061 gattccatca tcgatgccga cgtgaaaaac gaagactcca actacgaaga aaaattctgg
    21121 caggtattgg acaaaggcgt ttcagacagt ctctcctaca ttgccgccca aaccgttgcc
    21181 aaccctttcg gcgtggaaca atttatcgtc aacgccgagc aaactttcgc cggcagcttc
    21241 aaagccctca gcggcagcca aaccgactgg caagtctcca attctttcga ggtcgaagtc
    21301 ggcggcacgc ccaaaacctt tgaaaaacgg gttattgtta ccaccagccg cgattatcag
    21361 ggcttggaag cagtgaaagc cgcaggccgc gccttgtcgg aaaaagtcgc aggcgttgtg
    21421 tttgaaacct tgctggacgc gcacaaagca ggctggctgc accgttggga aatcgccgac
    21481 gtggtcatcg aaggcaacga cgaagcgcag caaggcatcc gtttcaatct gttccaactg
    21541 ttctccacct actacggcga agacgcgcgt ctgaacatcg gcccgaaagg ctttaccgga
    21601 gaaaaatacg gcggcgcgac ctattgggac accgaagcct acgccgtgcc gctctacctc
    21661 gcactggcgg aacccgaagt tacccgcaac ctgctgcaat accgccgcaa ccaactgcca
    21721 caggcgcaac acaacgcgcg cgaacagggc ttggcgggcg cactctatcc gatggtaacg
    21781 tttacgggca tcgagtgcca caacgaatgg gaaatcacct tcgaggaaat ccaccgcaac
    21841 ggcgcgattc cttacgccat ctacaactac accaactaca ccggcgacga gggctatctt
    21901 gccaaagaag gcttggaagt cttggtcgaa gtgtcccgct tctgggcgga ccgcgtccac
    21961 ttctccaaac gcagcggcaa atacatgatt cacggcgtaa ccggcccgaa cgaatacgaa
    22021 aacaacatca acaacaactg gtacaccaac accctcgccg catgggtatt ggactacacc
    22081 cgcgaagccc tggcgaaata cccgcgtccg gatttgaacg tgagcgccgc cgagttggaa
    22141 aaatgggcgg acatcagcgc gaatatgtac cgtccgtatg acgaagaact cggcgtattc
    22201 gtgcaacacg acggcttcct cgacaaaggc atccgccccg tgtccgcgct ttcgcccgac
    22261 gatttgccgc tcaaccagaa atggtcgtgg gacaaaatcc tgcgttcgcc ctttatcaaa
    22321 caggcggacg tattgcaagg catctatttc ttcggcgacc gtttcaatat cgacgaaaaa
    22381 cgccgcaact tcgacttcta cgaaccgatg accgtgcatg aaagctcgtt gtcgccttgt
    22441 atccactcta ttctcgccgc cgaactgggc aaagaagaaa aagccgtgga aatgtaccag
    22501 cgcaccgccc gcctggactt ggacaactac aacaacgaca ccgaagacgg cctgcacatt
    22561 acttctatga ccggctcgtg gctcgccatc gtccaaggtt tcgcccaaat gaaaacttgg
    22621 ggcggtaaac tcagcttcgc accgttcctg ccgggcgcat ggacaggcta cgccttccat
    22681 atcaactacc gaggccgtct gattaaagtc gccgtcggca aagaaaacgt cgtcttcacc
    22741 ctgctcaaag gcgagccgct cgatttgcag gtgtatggca aagacatcac gctcgacggc
    22801 agccacgccg ccgcgttgga aaaataagga gggtgcaaaa tgacgtttac cgcagtcctc
    22861 ttcgatctcg acggtgtcat caccgacacc gccgaatacc actaccgcgc gtggaaaaaa
    22921 ctcgccgaag aactgggcat cggcatcgac cgcaagttta acgagcagct caaaggcgtg
    22981 tcgcgcgacg attcgctcaa acgcatcctc gcgcacggcg gtaaaaccgt cggcgaagcc
    23041 gagttcgccg aactgacccg ccgcaaaaac gacaactact tggagatgat acaggcagtc
    23101 aaacccgaag acgtgtatct tggcattttg ccgctgctgg aagcattaaa agcaaacggc
    23161 aaaaaaatcg cccttgcgtc cgccagtaaa aacggcccct tcctgctcga acgcatgggg
    23221 ctgacccact tcttcgacac cgtcgccgac cctgccgccg tcgcgcattc caaacccgcc
    23281 cccgacatct tcctcgccgc cgccgagggc gtgggcgcgg acatccgccg ctgcatcggc
    23341 attgaagacg ctgccgcagg tgtcgccgcc atcaaagccg ccggcgcctt gcctatcggc
    23401 gtgggcaaag ccgaagactt gggcagcgac atcgcgctgg tgtccgatac cgccgagctg
    23461 acctacgcct acctgcaaaa cgtgtgggca cagtcgggca ggtaaaacgc gtcagataaa
    23521 gtgtcaagga agtaaaaggc cgtctgaaca gtgtttcaga cggcctttct gcttttagaa
    23581 cagattgata acccaactta cgcaacccta aaaactaaat gccaatttct taaccatgct
    23641 attcaaattt atttgaacga ttttttttct aaccagccaa ccttaacaat cactattaaa
    23701 atgcgcgccg atgttctgtc tccgcctgta tgcggcttgg gcgacggcga ggctgcattc
    23761 gagcaggttg cggttttcgt attcggacgc ggtgtgcggt tcggtttggt tttgtttcca
    23821 aagccgcagc tgggcgatgg cgcagcgcag accggtatcg ttgcggagga tgcccagatg
    23881 gcgttggttg aacgcttgca ggacggggcg gctgaatgcg ctttggatgc cgtctgaaaa
    23941 gatgcctgct tcggaggaga ggctttcaga cggccttgga gacgttttgg cttggaatgc
    24001 ttgtccgtct gcgatggttt gggcggcaag tctggcggta acgacacatt cgagcaggga
    24061 gttgctggca aggcggttgg ctccgtgcaa gccggtgcag gcggtttcgc ctaaggagta
    24121 gagctgcggc agggaagttc tgccgcaggg gtcggtttgg atgccgccgc aggtgtagtg
    24181 ttgcacgggg cggacgggga tggcttggcg cgtgatatcc aggccgcatt gggataaaca
    24241 gtgccgatgg atggacggga aatgccggcg gacgaacgct gcgggttgat ggctgatatc
    24301 gagtgagacg aagtcttgcg tttgtttggc gatttcggcg gcgatggcgc gggcaacgat
    24361 gtcgcgcggc gcgagttcgg cgcggcggtc gtaatacggc ataaaccgtt cgcccgcttg
    24421 gttggtcagg atgccgcctt cgccgcgcac ggcttcggaa atcaggaagg tgcggccgtt
    24481 ttcagacgat cttgccaagc ctgtggggtg gaattggata aattcgaggt tttcgactgc
    24541 gcagcctgcg cgtatcgcca tggcgatggc gtcgcccgtg cattcgggcg gcgtggtggt
    24601 ggcggcgtaa atctgtccca agccgccgcc tgcgagtacg gtatggcggg cgcggatgct
    24661 gtaggttgct tgcgttcggc agtcgaggac ggtcagtccg cacgccgcac ctgattcggt
    24721 ttggatgtcc aacgccatct gctgctcgca aacgcggatg ttcgggcggc agcgtatttg
    24781 gacagtcagg ctctgcatga cggcttcgcc cgtgtagtcg gcgacgtggg cgattcggcg
    24841 gcaggtgtgc ccgccttcgc gcgtcaggtg caggccgtta ttattttggt cgaacgccac
    24901 gccctgcgcc agcagccatt cgattgccag tttgccctgc gacaggatgg tgcggacggc
    24961 ggcttcgtcg cacaaacccg cgcccgcctc caaggtatcg gcaacgtgtt tttcgatgtc
    25021 gtcctctccc gaccacgccg ccgcaatccc gccttgcgca tgacggctgg cagtgtcgtc
    25081 cagccggttt ttgcacaaaa tgacgatgcg gaacgattca ggcagcgaca gggcgagcgt
    25141 cagtgccgcc agcccgtttc cggcaatcaa tacgtcgcaa tcggtttgca tggtgttgtc
    25201 cttgtttgag agatcgtctg aaacggtata gtggattaaa tttaaaccag tacggcgttg
    25261 cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt
    25321 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
    25381 taatatggac ggcaaatcag gcgggtccca tgccgttgaa cacatccccg cgcttgagtc
    25441 ctgccgcgaa gtcgagcata cgctgcaaag gcagtttggc ggcttcgccc agcttcctgt
    25501 ccaacaggat ttcgttgtgt ccgcttgtca gggcgtattt gatgccgccc agcgaattca
    25561 tcgccatcca agggcagaac gcgcagcttt tacagcttcc accgttgccc gccgtcggtg
    25621 cggcgataaa ttctttgtcg ggtgcctgct tttgcatttc gtgcaggatg cccaagtcgg
    25681 tcgctacgat gaattttttt tcagggtgcg atacggcggc tttgagcagt ttgctggtcg
    25741 agccgaccac gtcgcccagt tcgatgacgc tttgtggcga ttcgggatga accagcacca
    25801 ccgcttcggg gtgttccgcc ttcaacgccg ccagctcctg ccctttgaat tcgttgtgga
    25861 cgatgcacga accctgccac agcagcatat ccgcgcccgt ttcgcggcgg atgtagtcgc
    25921 cgaggtggcg gtcaggtccc caaatcagct tctcgccgcg cgatttcaaa tacgatacga
    25981 tttccaacgc caccgaagac gttaccaccc aatcggcacg cgctttcacg gcggcggaag
    26041 tgttggcgta cactacgacc gtgcggtcgg ggtgttggtc gcaaaacgct gaaaacgctt
    26101 cttccggaca gcccaaatcc aaagaacatt ccgcctccaa atcaggcatc agcactgttt
    26161 tttcagggca gaggattttc gcgctctcgc ccatgaagcg cacacccgcc accaccaatg
    26221 tactagcttc gtgttccgcg ccgaagcgcg ccatttccag cgaatcgccc acgcatccgc
    26281 ccgtttctaa agccaaatcc tgaatcagcg gatcgacgta ataatgcgcc accaagaccg
    26341 cgtttttctc cttcagcaaa gccttgattt cgtctttcag acggcctgcc gtctcgcggt
    26401 cgggcgtgtc ggcaaccttc gcccacgcct gacggatttg gcaggcggaa gtcggggttt
    26461 ggatgagggg catatcgtaa tcgaacgagc ggcgggcggc ggtttgcatg agacttcctt
    26521 atggctggtt tttcagacgg cataaagatt tgccgtttat attttgaaat atttttatat
    26581 tatgctcaaa ttgagtataa taagcaaggt cgtctgaaaa caggtttgcg ataccgtaaa
    26641 accgacccgc ttcgttccga caaaccgctt tggtttatag tggattaaca aaaatcagga
    26701 caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgctgtactg
    26761 gtttaaattt aatccactat acaataaagc ctttcccacc cgcagaaagc cgagcatgga
    26821 tgcctacccc gaagccgaag ccccgccgca aagcatcgtc gagctggttc ccgtattgat
    26881 tgccgttacc gacggcggcc tgcgggtatt aaccgtcgcc caaggcacac tcctgcccaa
    26941 cggcccgctc tcccctctgc gcaattccct gcaggcgggc gtgaagctgt gggttgccaa
    27001 gcagacttcg cagcctatgg gctatgtgga acagctttac acctttgtcg atacccaccg
    27061 ccgcaacgaa cacggcatgc ccgtcttgta cgtcagctat ttggggctgg tgcgcgaggc
    27121 agccgacagc atcctgcacc cggatgcgaa atggcaggac tgctacggct atttcccgtg
    27181 ggaagacttg cgcaccgacg gcgggcagcg cgacgccgtc gtcagccgcc tgcgcatttg
    27241 ggcaaattcg gcggacacgg aggaagtgcg tcaaatgcgg ctcaagcgca ttcatttgtg
    27301 ctggggggtc gaaccggaaa actggtcgga agaatacgtt ttacaacgct atgaaatgct
    27361 gtatgaaagc ggtctgatag tggaagccgc cgagccgcag gcaaacttcg acttcgcgct
    27421 cacggggcag cccatgcgcc acgatcaccg tcgcgtactg gcgaccgccc tgtcccgcct
    27481 gcgcgccaaa atcaaatacc gccccgtgat ttttgaactg atgccgcccg aattcacgct
    27541 gctgcaactg caaaacagtg tcgaagccat cagcggaaga ttgctgcaca agcaaaactt
    27601 ccgccgccag attcagcagc aaaaccttat cgagccgtcg gataccggcg tatcgggcag
    27661 caaaggccgt cccgcgcagc tttgccgctt ccgcgacgac gtcctgcccg acaggctgat
    27721 ttcggacatc ggattgccgc tgggcagccg ttagcccgtt ttcagacggc atcaaatgaa
    27781 gttttgcccc atcggtgcaa catcaatctt tttcgacaaa ggaaacctca tgccgtctga
    27841 aaacaccctc ttttccctgc ccgacaccct gttgcgcccc atagtcgaac aagccttgag
    27901 cgaagacttg ggcaggcgcg gcgacattac gtccgccgcc gttatcgccc ccgacaaaac
    27961 cgccaaactc ttcctcgtca gccgcgaaga cggcgttatc gccggcatgg atttagcgcg
    28021 cctcgccttt cagacgatgg atccgtccgt ccgcttccaa gccgaaatcc gagacgggca
    28081 agccgtccgc gcaggtcaga cgcttgccgc cgtcgaaggc aacgcccgcg cgctgctcgc
    28141 cgccgaacgc accgcgctca actacctcac gcacttaagc ggcatcgcca ccgccaccgc
    28201 gcgtgccgtt gccgaagtcg ccgaatacgg tacagacatc gtgtgcagcc gcaaaaccat
    28261 ccccctgctg cgtgtcctgc aaaaatacgc cgtcagggca ggcggcggtg tgaaccaccg
    28321 catgggtttg gacgacgccg tgctcatcaa agacaaccac ctcgcctatt gcggcagcat
    28381 cgcccaagcc gtgcagcagg caaaacaggc tgtcggagca ttgacctgcg tggaaatcga
    28441 agtggatacg ttggcacaac tggacgaagc catcgcagcg ggcgcggaac ggattttgct
    28501 ggataacatg gacgacgaaa ccctgaaaga agcggcaaac cgctgccaca cgcaaaccgc
    28561 ccacccccac accatctatt gcgaagcatc gggcggcatc ggcttcgacc gcctgaagcg
    28621 cgtggcgcaa accggagtgg acggcatcgc cctcggctat ctgacccaca gcagccgttc
    28681 gttggacata ggtttggatt tcgtggcgtg agttttaggg tgcgggcggc tgtctgatat
    28741 gtcaggcaag gaaccgctta accctaatcc ggttattgcc tcagggagga aatgccgtct
    28801 gaaagattct tcagacggca tttttcgtaa aggtcgtgat gctttagaaa aaacagcatt
    28861 tcaggcaggt attttgtttg cccgacagcg cggcggcatc ggtagggcag gaaaaaggac
    28921 ggggggcggc agttttatgc cgtctgaaag cccgccttta cgcttgtttg caaaaaagtg
    28981 ggaaaaggaa catacaatcc tgtacaatca tccataaata tttgatttat aatacgattt
    29041 ataaagataa tcacaatcat ccatatctgc cgcccgtcaa tccgcttggc gggcggcaaa
    29101 ggttttagga ataccgatga acacaatacc gctccacacc atactcaaac ttatggcgca
    29161 tcccgaacgt atggcgatac tgattcaatt gttggacagc gaacgcaata tcgccgaact
    29221 ggcaaaatcc ttatccctgc cggccaccgc agtttccaac catttgaacc gcctgcgcgt
    29281 ggaaggtcta gtcgatttta cgcgttacca ccgcattatc gaataccgcc tggtttccga
    29341 agaagcggcg gcgattctgc acacggttcg cgatttggaa aacaaacgcg tggcatagtg
    29401 ttagaatcct ttccttttgc cgtctgaacg tttcagacag catttttcgg aaatgttatg
    29461 aaaatcacca cttggaatgt caattcgctc aatgtgcggc tgccgcaggt gcaaaacctg
    29521 cttgccgaca atccgcccga tattttggtt ttgcaggaac tcaaactcga tcaggacaaa
    29581 tttccggccg ccgctttgca aatgatgggc tggcactgtg tttggagcgg gcagaaaacc
    29641 tacaacggcg tggcaatcgt cagccgcagc gtgccgcagg acgtgcattt cggtttgccc
    29701 gcactgccgg acgatccgca acggcgcgtg attgcggcaa ccgtcagcgg cgtgcgcgtc
    29761 atcaatgtct attgcgtcaa cggcgaggct ttggacagcc ccaaattcaa atataaggaa
    29821 cagtggtttg ccgcactgac ggagtttgtc cgcgatgaaa tgacccgcca cggcaaactg
    29881 gtgttgctgg gcgatttcaa tatcgcgcct gccgatgcgg actgttacga ccctgaaaaa
    29941 tggcacgaaa aaatccactg ttcgtccgtc gaacggcagt ggtttcaaaa cctgctggat
    30001 ttgggactga ccgacagcct gcgccaagtc catcccgaag gcgcgttcta tacctggttc
    30061 gactatcgcg gcgcgatgtt ccaacgcaaa ctgggcctgc gtatcgacca tattttggtg
    30121 tcgcctgcga tggcggcggc gttgaaggat gtccgcgtcg atttggagac gcgcgcgctg
    30181 gagcgtccga gcgaccacgc gccggtgacg gcagaattcg attggtaaaa gaccgtgttt
    30241 tgatatggcg ttgacaaaca tccatatctt taagtgattc aataggatag gctgctgact
    30301 aaccgaaaat aattgccttt tccctgacaa acagccggag tcggcggatt gttcaagcac
    30361 agcctatttt tctgaaaaat ttatgaaatc catagggtta atatcagatt ttggagcagt
    30421 aaaatttatt atgtatataa tccggagcag aatcaaatat taaaaaccgg atttatttta
    30481 gaatgaatag tcaggctgcc ttatgtatag taataaatta atctcctttt tttcctattg
    30541 cctttattgt gctatgcagt tgagtttgat gaaacccaat ataacgactg taaagatgaa
    30601 cctatgttat gtgctgtcag aattgattct cccaaaggca ataactatag tggattaaca
    30661 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa
    30721 cgacgtactg gtttaaattt aatccactat ataaatctat gtggtttgac aatggcaagt
    30781 tagtatttat atcctttact aatcaacaaa tggaaaatca aagtcgccca tctctagcga
    30841 tgtttattag tgatgacaaa atatccagta ccaatattga tgaattttta gcatctttcg
    30901 atcctgataa atatcgaata tttcatgatc caagatataa atttttacct agtatgtcga
    30961 actcattgta atccttattc tctttttgat attgatagca aatataaacc tgatgagaaa
    31021 gataaaatct ttttttcaat cccgacagat aacacagatt tttataaggg tttttattta
    31081 aataaggatt atatagaagg tatatatcct agtaggcata atggcagcta ttacaaaata
    31141 tagtggatta aatttaaacc agtacagcgt tgccgtacta tttgtactgt ctgcggcttc
    31201 gtcgccttgt cctgattttt gttaatccac tat
//
LOCUS       DAXIHS010000016        29180 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXIHS010000016 DAXIHS010000000
VERSION     DAXIHS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.016"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            1..148
                     /locus_tag="ACFOF2_000618"
                     /pseudo
     CDS             1..148
                     /locus_tag="ACFOF2_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(158..1267)
                     /gene="mltB"
                     /locus_tag="ACFOF2_000619"
     CDS             complement(158..1267)
                     /gene="mltB"
                     /locus_tag="ACFOF2_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC7106980.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1176..1427)
                     /locus_tag="ACFOF2_000620"
     CDS             complement(1176..1427)
                     /locus_tag="ACFOF2_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7106981.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1468..2895)
                     /locus_tag="ACFOF2_000621"
     CDS             complement(1468..2895)
                     /locus_tag="ACFOF2_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC7106982.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3080..7192
                     /gene="mfd"
                     /locus_tag="ACFOF2_000622"
     CDS             3080..7192
                     /gene="mfd"
                     /locus_tag="ACFOF2_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC7106983.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKAKSSLHLVASNLH
                     GQIRQQPTPSPVGEGWGEGKAVSAQTEFSAAAISPLPSPLPQEREQSAAAVSDDLKTK
                     SSLHPVASNLHGQIRQQPTPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHN
                     RRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGE
                     AQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAR
                     RAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGF
                     GKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSK
                     ATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLREL
                     KRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITK
                     DAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQA
                     VRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQIN
                     AIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPT
                     EIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7341..7724
                     /gene="panD"
                     /locus_tag="ACFOF2_000623"
     CDS             7341..7724
                     /gene="panD"
                     /locus_tag="ACFOF2_000623"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC7106984.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7746..8588
                     /gene="kdsA"
                     /locus_tag="ACFOF2_000624"
     CDS             7746..8588
                     /gene="kdsA"
                     /locus_tag="ACFOF2_000624"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC7106985.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8603..9043
                     /locus_tag="ACFOF2_000625"
     CDS             8603..9043
                     /locus_tag="ACFOF2_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC7106986.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            9091..10377
                     /gene="eno"
                     /locus_tag="ACFOF2_000626"
     CDS             9091..10377
                     /gene="eno"
                     /locus_tag="ACFOF2_000626"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC7106987.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            10391..10669
                     /gene="ftsB"
                     /locus_tag="ACFOF2_000627"
     CDS             10391..10669
                     /gene="ftsB"
                     /locus_tag="ACFOF2_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC7106988.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10710..11000)
                     /gene="yfaE"
                     /locus_tag="ACFOF2_000628"
     CDS             complement(10710..11000)
                     /gene="yfaE"
                     /locus_tag="ACFOF2_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC7106989.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(11198..12331)
                     /gene="nrdB"
                     /locus_tag="ACFOF2_000629"
     CDS             complement(11198..12331)
                     /gene="nrdB"
                     /locus_tag="ACFOF2_000629"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC7106990.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12392..13579)
                     /locus_tag="ACFOF2_000630"
     CDS             complement(12392..13579)
                     /locus_tag="ACFOF2_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC7106991.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(13576..14586)
                     /gene="dcm"
                     /locus_tag="ACFOF2_000631"
     CDS             complement(13576..14586)
                     /gene="dcm"
                     /locus_tag="ACFOF2_000631"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC7106992.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KK"
     gene            complement(14644..16923)
                     /gene="nrdA"
                     /locus_tag="ACFOF2_000632"
     CDS             complement(14644..16923)
                     /gene="nrdA"
                     /locus_tag="ACFOF2_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC7106993.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDDSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17452..17763
                     /locus_tag="ACFOF2_000633"
     CDS             17452..17763
                     /locus_tag="ACFOF2_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC7106994.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            complement(18075..18842)
                     /locus_tag="ACFOF2_000634"
     CDS             complement(18075..18842)
                     /locus_tag="ACFOF2_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC7106995.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(18930..19757)
                     /gene="mutM"
                     /locus_tag="ACFOF2_000635"
     CDS             complement(18930..19757)
                     /gene="mutM"
                     /locus_tag="ACFOF2_000635"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC7106996.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(19809..20474)
                     /locus_tag="ACFOF2_000636"
     CDS             complement(19809..20474)
                     /locus_tag="ACFOF2_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC7106997.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            20653..22629
                     /locus_tag="ACFOF2_000637"
     CDS             20653..22629
                     /locus_tag="ACFOF2_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC7106998.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASRSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            22697..23389
                     /locus_tag="ACFOF2_000638"
     CDS             22697..23389
                     /locus_tag="ACFOF2_000638"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC7106999.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLESPSALLLIITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKL"
     gene            complement(23590..23696)
                     /locus_tag="ACFOF2_000639"
                     /pseudo
     CDS             complement(23590..23696)
                     /locus_tag="ACFOF2_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            24019..25334
                     /locus_tag="ACFOF2_000640"
                     /pseudo
     CDS             24019..25334
                     /locus_tag="ACFOF2_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            25348..25482
                     /locus_tag="ACFOF2_000641"
     CDS             25348..25482
                     /locus_tag="ACFOF2_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107000.1"
                     /translation="MPSESHTSFRRHCVDARHNTRNARIRTGLPASVGYFRAGGVPSA
                     "
     gene            25578..26234
                     /gene="cmk"
                     /locus_tag="ACFOF2_000642"
     CDS             25578..26234
                     /gene="cmk"
                     /locus_tag="ACFOF2_000642"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC7107001.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26389..28074
                     /gene="rpsA"
                     /locus_tag="ACFOF2_000643"
     CDS             26389..28074
                     /gene="rpsA"
                     /locus_tag="ACFOF2_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC7107002.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28085..28399
                     /locus_tag="ACFOF2_000644"
     CDS             28085..28399
                     /locus_tag="ACFOF2_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC7107003.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(28750..29157)
                     /locus_tag="ACFOF2_000645"
     CDS             complement(28750..29157)
                     /locus_tag="ACFOF2_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC7107004.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
BASE COUNT         7447 a         8027 c         6787 g         6919 t
ORIGIN      
        1 tgcaaaaatg ccgtccgaac cgaaacgtgg ttcagacggc attttatagt ggattaacaa
       61 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcaaggcaac
      121 gccgtactgg tttaaattta atccactata aaaaacatta caatcccggg ccgccaagcg
      181 aattggcaat gtccctgacc gccgttacat acatccgact gtgattgtac tgccataccg
      241 tataaaaatt gttcaagccc aaataatatt caaacacgcc gggtgcggtt tccagtttga
      301 acaaaaccgc cttttcatca tctgcaagct cttcgccggg gatgatgccg tacgccttta
      361 aatccgccac cgtccgcgtc agggcggttt tttcgccaat gattgcctga acatccgcac
      421 ccggcgccaa tgttgcagac accagtattt tcccgcccgt gcgccaaccg tgctgcttca
      481 tataattggc aaccgatgcc gcgacatcgc cgatgttgcc ccatatgtcc cgatgtccgt
      541 ccccgtcata atccaccgcc catttccggt agctcgaagg cataaattgc ggcatcccca
      601 ttgcgcccgc atagctgcct ttaaaggcga aaacatcgcc gccttcttct tttgccagct
      661 ttaaaagctc gaccaattct ttttggaaaa acccggcgcg gcgggggtaa tcaaagccta
      721 aggtcgccaa tgcgtccgcc acacggaaac tgcccgtatt tttgccgtaa ttcgtttcaa
      781 tcccgataac cgccacgata agttcggcag gcacgccgta tttttgcgcc acatcatcga
      841 taagcgcgcg gttttccgca taaaaccggc gcgcgccgcg aaatttcgcc ttgcccgaat
      901 ttcccgtgcg gaacacatac cacggacgcg atgtggaggg gcggtgcata atcttgacga
      961 tgtccgcctt gtaagccgct ttgtcaaaaa aatcctgcca ttccgcccgg gaaaaatccc
     1021 ctttcccgac ttcatcgtcc acaaaacggc ggacatttgc attggcggca aacccgctgt
     1081 cggataccgg tacggctgcc gcgtcaaata cggctgccgc gtcaaacgcg gggcggcttt
     1141 cttttttcat ttcatccgcg cggggggctt gggcttcatt tgcccggggc gggcgtgcct
     1201 ccatcgccgt acaggcagac aaagccgcca aacaaattgc cagcggcagt atttttctct
     1261 ttttcataaa tatgttccga acaaataggg taagtgggaa agcggcacaa gggggcggcg
     1321 cgaaatgcgg catccgccgc aatcggcggc tttgcggaat gccgcgcgtt gccttttgcg
     1381 ccgcccgaaa tctgcctgcc gccgccgaaa atgccgtcta aaggcacttc ccctttcaga
     1441 cggcattgcc tgcgccgtat ttgcccgcta tccgcaatat cggcagtcca atatgtcttt
     1501 gcggatatcg ttcagcaggc aggctgccgc gtcttcgtgt tccgcctgta cgaagacaaa
     1561 gagtcgggcg atgattttgc cgataaagac tttttgcagg gcgcaggcgt cggtcagcgt
     1621 gtgttcctgc aggaagctgc ggatgtcttc gggtaaggtg taatcacggg caacggcggc
     1681 aaagcgggca tcggtttgca ggcggtcgag cagggtttgg tttttgtcca acttcatgcc
     1741 tgcttctttt tcttcggcgg tggcgcggac aaactggtcg taaatttcgg cataaagcat
     1801 atcgttcggg ccttcaaaaa tcgtgaaggg gcggatgtcg atagcgatat tgccggcggt
     1861 gtgtccgcgt tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg cggcggcgta
     1921 agtgtattcc gtggcgaggg ttttgacgat gttcgcctcc atcagctgat gggcgacggg
     1981 ggcaacaggc gaaacggaat ggcagacgta gcggtaaaga atctcggaaa cctgatggcg
     2041 gcgccggatt tcgcggcgtt cgtaatcgac gaatctgatg tcgttgcgga cgtatcgttc
     2101 caggttttca aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt gcaggcggct
     2161 gcggataaag atgttttgga acgcgcgcaa accggcagcg tcgctctggg agagtttcat
     2221 cacggcggtt gcaggcattt cggcatcgat gcggttgacg gcgtaacgga cggcgcgcaa
     2281 gccttcggat gcgagggttt cgcagcggat gtatgttttg gggacgagca gcaggtcgat
     2341 gactttggcg agtttgccgt ttttgcgttc tttggcggca acgaggagga agtcgctttg
     2401 cgagttgccc tgccagtatt ttgcggcgtt gacgtaaatg gtttgtccgt cggtatattc
     2461 gtagtaagac tgcatttctc gggcaatcgc cgcgccggag gtttcgggtt cggtaacgcc
     2521 caaaccaccg ccctcgcctt tgaaaaccat gtccaaaccc tgtgcgattt gcgcttcatc
     2581 gccgaactct tgcagtggct gcaacaccag cgcgccttcg atgccggtac gcagcgtaac
     2641 gggcacgccg taatgccccg caatccgcaa gacttcctga atttcaaact ggctgccctt
     2701 gcgcccgccg tgttttttgt cgaggaaggg caacagcaaa cccgcctgct tcaaggcaag
     2761 ccatttgtct tcgggcaggt atcgcatcag gtcgataccg tctgaaaaaa tgcggcggaa
     2821 tgcggattcg atgtgcttta aaaaagcagc cgtgtccata gttgacggct gcgcgctcgg
     2881 ttcggtgtgt atcatcggct tcctctgtcg gttcccatta atcggcggcc ggtcaaaccg
     2941 cctgccacag tttagagttg attttctaaa ctttaccaca aagtgcgccg ggcaacaatc
     3001 cgccgacctt tcagacggca tcgcgccccc tcccgtgcta aaatgaccgt ttgcatcact
     3061 gtccgccgat tgccgcacta tgacctaccc catccccaaa ccccgtgaaa aatcccgttg
     3121 gtccaatctt tcgcaaggct cgctgccctt ggctttggcg cgttatctgc cgcataagcg
     3181 gctcaaggtc gtgctgaccc aagatgcgga acaggcgcta cgccttcaga cggcatggcg
     3241 gtttttccgt ccgcacgaca cggcggtgtt cctgccggac tgggaaacgc tgccttacga
     3301 gcgtttttcg ccgcatcagg atttggtgtc ggaacggctg tcggcgttgt ggcagattaa
     3361 gagcggcgcg gcggacgtgt tgttcgtgcc ggttgccacg gcgatgcaga agctgccgcc
     3421 cgtgccgttt ctggcagggc gcacgttttg gctgaaaaca gggcagactt tggatatagg
     3481 ccgtctgaaa accgatttgg tagatgcggg ctacaaccat gtttcccatg ttgttgcggc
     3541 gggcgagttt gccgtgcgcg gcggcattgt cgatttgttc cccatgggca gcgaaatgcc
     3601 ctaccgcatc gatttgtttg acgatgaaat cgacagcatc aaaacctttg atacggacac
     3661 gcagcgcacc atctcccccg tttccgaaat ccgcctgctg ccggcgcacg aattccccac
     3721 cgacagcgag gcgcaaaaaa tcttccgcag ccgcttccgc gaggaagtcg acggtaatcc
     3781 gaacgatgcc gccgtgtaca aagccgtcag caacggccac ttcggtgcgg gcgtggaata
     3841 ttacctgccg ctgttttttg aaaacgagct ggaaacgctg tttgactata tcggcgagga
     3901 tgcgctgttt gtctctttgg gcgatgttca cgccgaggca aaccgctttt ggagcgacgt
     3961 caaatcgcgt tacgccatgg cgcagggcga tgaaacttat ccgcctttgc ttccacagca
     4021 tttgtatctc tctgccgatg tattcgcagg ccgtctgaaa aactacggac aagtgctgcc
     4081 cgatgtttcc ggcaaggaac acatcctgcc cgaccttgcc gtcaaccgcc aatccgacga
     4141 gccgttgcag gcattgaagg attttcagac gacctttgac gggcggattt tgctgtgcgc
     4201 cgaaagcttg ggacggcgcg aaaccatgct cggtttcttg cagcaaaacg gtttgaaagc
     4261 caaacctgtg tccgactggc agggcttctt gtcggcgcat gagccgctga tgattacagt
     4321 ggcgccgttg gcttacgggt ttaaactggg cggactgcaa tcgccgaacc aacagcaaac
     4381 tacttccgcc tccgaaggag aaggcgatgc ggttaccgac caaaccgaat tttccgcatc
     4441 cgcaacaaac cctctcccta gccctctccc acaggagagg gaacaaagtg cagccgtcgt
     4501 ttcagacagt ctgaaagcag ccgccgtttc aaccgaaagc agcctgcccc ccggtaaaag
     4561 taatctgcac gggcaaatcc aacagcaacc tgccccctcc cccgtggggg agggttgggg
     4621 agagggcaaa gcagttgccg ctcaaaccga atttcccgca tccgcaacaa accctctccc
     4681 tagccctctc ccacaggaga gggaacaaag tgcagccgcc gtttcagacg acctgaaagc
     4741 caaaagcagc ctgcatctcg tcgcaagtaa tctgcacggg caaatccgac agcaacctac
     4801 tccctccccc gtgggggagg gttggggaga gggcaaagca gtttccgctc aaactgaatt
     4861 ttccgcagcc gcaataagcc ctctccctag ccctctcccg caggagaggg aacaaagtgc
     4921 cgccgccgtt tcagacgacc tgaaaaccaa aagcagcctg catcccgtcg caagtaatct
     4981 gcacgggcaa atccgacagc aacctactcc ctcccccgtg ggggagggtt ggggagaggg
     5041 caaagcagtt gccgctcaaa gtgccatcgc cgtcatcacc gaatccgatc tctaccaata
     5101 cgtcgcccgt tcgcgcgtcc acaaccgccg caagaaacac gccgccgttt cagacggtct
     5161 gttgcgcgac cttgccgaaa tcaacatcgg cgaccccgtc gttcacgaag aacacggcat
     5221 cgggcggtat atgggcttga tcacaatgga tttgggcggc gaaaccaacg aaatgatgtt
     5281 gcttgaatat gcaggcgaag cgcagcttta tgtgcctgtt tcgcaactgc atttaatcag
     5341 ccgctactcc ggtcaggcgc atgaaaacgt cgccctgcac aagctcggca gcggcgcgtg
     5401 gaacaaagcg aaacgcaaag ccgccgaaaa agcgcgcgat accgccgccg agttgcttaa
     5461 cctttacgcc cgacgcgctg cccaatcggg acacaagttt gaaatcaacg agttggatta
     5521 tcaggcgttt gccgacggct tcggctacga agaaaccgaa gaccaggcag ccgccatcgc
     5581 cgccgtgatt aaagatttga cgcaggcgaa gccgatggac cgcctcgtgt gcggcgatgt
     5641 cggtttcggc aaaaccgaag tcgccctgcg cgccgcgttt gtggcggtaa tgggtggcaa
     5701 acaagttgcc gtactcgcac cgaccacgct tctggtcgag cagcacgcgc aaaattttgc
     5761 cgaccgcttc gccgatttcc ccgtgaaagt cgccagcctt tcgcgtttca acaacagcaa
     5821 agccaccaaa gccgcgctgg aaggcatggc ggacggcacg gtcgacatcg ttatcggcac
     5881 gcacaaactg gtgcaggacg acatcaaatt caaaaactta ggtttagtga ttatcgacga
     5941 agaacaccgc ttcggtgtgc gccaaaaaga gcagctcaaa cgcctgcgtg ccaatgtcga
     6001 catcctcacc atgaccgcca cgccgattcc gcgtacttta agtatggcgt tggaaggact
     6061 gcgcgacttc tcgctgatta ccaccgcgcc cagccgccgc ctcgccgtca aaacctttgt
     6121 caaacccttt agcgaaggca gcgtgcgcga agccgtgttg cgcgaactca aacgcggagg
     6181 acaggtattt ttcctgcaca atgaagtaga tacgattgaa aatatgcgcg agcggctgga
     6241 aaccctgctg cccgaagccc gcatcggcgt ggcgcacgga caactgcgcg agcgcgagct
     6301 ggaacaagtc atgcgcgact ttttacagca acgattcaac gtgttgctct gttccaccat
     6361 catcgaaacc ggtatcgaca ttcccaacgc caacaccatc atcatcaacc gcgccgacaa
     6421 attcggactg gcgcaactgc accagcttcg cgggcgcgtc ggacgcagcc accaccaagc
     6481 ctacgcctac ctgctcacgc ccgaatacat caccaaagac gcagaaaaac gcctcgacgc
     6541 catcgcggca gcagacgaac tcggcgcagg tttcaccctc gccatgcagg atttggaaat
     6601 ccgcggcgca ggcgaaatcc tcggcgaagg gcagtccggc gaaatgatac aggtcggctt
     6661 cacgctctac accgaaatgc tcaaacaagc cgtgcgcgat ctcaaaaaag gccgccaacc
     6721 cgacctcgac gcgccgctgg gcatcaccac cgaaatcaaa ctgcacagcc ccgccctact
     6781 gcccgaaagc tactgccccg acatccacga gcgtctcgtc ctctacaaac gcctcgccgt
     6841 ctgcgaaacc gtgcagcaaa tcaacgccat acacgaagaa ctcgtcgacc gcttcggcct
     6901 gcccgaacaa cccgtcaaaa cccttatcga aagccaccac ctgcggctta tggcaaaaga
     6961 attgggtatc gatgccattg atgcggccgg cgaagcggta acggtaacct tcggtaaaaa
     7021 caataatgtc gatccaaccg aaatcatcct gctgattcag aacgacaaaa aataccgcct
     7081 tgccggcgcc gataagctgc ggtttaccgc agagatggaa aatatcgagg tcagaatcaa
     7141 caccgtaaaa aacgttttaa aaaccttgca aaacagatgc ctgcccaaat aaagccgaca
     7201 ccgcaatgcc gtctgaaaca ccgttttcct tgtccgaaag ccgccattat gaatttgaag
     7261 gaaactccac tataatacgg cattcagatt tccagacggc atcgcgcccg tcagaccgca
     7321 cacaaaccaa aaggaaatac atgttccgta ctatgcttgg cggaaaaatc caccgcgcca
     7381 ccgtaaccga agccgatttg aactacgtcg gcagcattac cgtcgatcaa gatctgttag
     7441 acgcggcagg catctacccc aacgaaaaag tcgccatcgt caacaacaac aacggcgaac
     7501 gttttgaaac ctataccatt tcaggtaaac gcggcagcgg cgtgatttgt ctgaacggtg
     7561 ctgcggcaag gctggtgcag aaaggcgata tcgtcatcat catgtcttac gtccaactct
     7621 ccgaacccga aatcgccgcg catgaaccca aagtcgtctt ggtagacgga aacaacaaaa
     7681 tccgcgacat catctcctac gagccgccgc acaccgtgct gtaaccctga aaacggacat
     7741 tgatcatgga tattaaaatt aacggcatta ccgtcggtaa cgatgctcct ttcgtactgt
     7801 tcggcggcat caacgtttta gaagatttgg attccaccct ccaaacctgt acgcattacg
     7861 tcgaagttac ccgaaaactc ggtattccct atatctttaa agcatctttc gacaaggcaa
     7921 accgttcctc catccattct tatcgcggcg taggcttgga agaaggctta aagatttttg
     7981 aaaaagtcaa agcggaattc ggcatccccg tcattaccga cgtacacgaa ccctatcagt
     8041 gccaacccgt cgccgaagtg tgcgatgtca tccagcttcc cgcattcctt gcgcggcaga
     8101 ccgatttagt ggttgccatg gcaaaaaccg gcaacgtcgt caacatcaaa aaacctcagt
     8161 tcctcagccc ctctcaaatg aaaaacattg tggaaaaatt ccacgaagcc gacaacggga
     8221 aactgatttt atgcgaacgc ggcagcagct tcggctacga caacctcgtt gtcgatatac
     8281 tcggtttcgg cgtgatgaaa cagacttgcg gcaacctgcc ggttattttc gacgttaccc
     8341 attccctgca aacccgcgat gccggttctg ccgcatccgg cggtcgtcgc gcacaggctt
     8401 tggatttggc acttgcaggc atggcaaccc gccttgccgg tctgttcctc gaatcgcacc
     8461 ccgatccgaa actggcaaaa tgcgacggcc ccagcgcgct gccgctgcac cttttagaag
     8521 attttttaat ccgcatcaaa gcattggacg atttaatcaa atcacaaccg attttaacaa
     8581 tcgagtaaca cggtttcgcc ttatgatgca gactttccga aaaatcagcc ggtatgtcgc
     8641 aaccttgtgg ctcggtatgc agattatggc gggttatatc gccgcaccgg tgctgttcaa
     8701 aatgctgccc aaaatgcagg cgggcgaaat tgccggcgta ttgttcgaca tcctctcttg
     8761 gagcgggctt gccgtttggg gcgcggtgtt ggctgccgcc tttgccatcc tgacccgact
     8821 gcaaaccgcc ctgctgcttt ttttattgtc cgcccttgcc gccaaccaat ttttggttac
     8881 ccccgttatc gaggcactga aatacgggca tgaaaattgg ctgttgtcgt ttgtaggcgg
     8941 atccttcgga atgtggcacg gtatttccag catgattttc atggcaaccg ccctactttc
     9001 agcagtttta agttggcggc tttccggcaa agatgccgtc tgaagccctc ccattttttt
     9061 acctcccttc acttcacttg gagaacattc atgagcgcaa tcgttgatat tttcgcccgc
     9121 gaaattttgg actcacgcgg caaccccaca gtcgagtgtg atgtattgct cgaatccggc
     9181 gtaatgggac gcgcagccgt accgagcggc gcgtccaccg gtcaaaaaga ggctttggaa
     9241 cttcgcgacg gcgacaaatc ccgttattcg ggcaagggcg tattgaaggc ggtcgaacac
     9301 gtcaacaacc aaatcgccca agccctcatt ggtatcgatg ccaacgagca atcttatatc
     9361 gaccaaatca tgatcgaatt ggacggtact gaaaacaaag gcaatttggg tgcgaatgcg
     9421 actttggcgg tttctatggc ggttgcacgc gccgctgccg aagactcagg cctgccgctt
     9481 taccgctact tgggcggcgc aggcccgatg tccctgcccg taccgatgat gaacgtcatc
     9541 aacggcggcg aacacgccaa caacagcctg aacatccaag agtttatgat tatgcccgtc
     9601 ggcgcaaaat ctttccgcga agcgttgcgc tgcggtgcgg aaattttcca cgccttgaaa
     9661 aaactgtgcg acagcaaagg cttcccgacc acagtcggcg acgaaggcgg tttcgccccc
     9721 aacctgaaca gccacaaaga agccctgcaa ctgatggtcg aggcgaccga agccgccggc
     9781 tacaaagcgg gcgaagacgt attattcgca ttggactgcg cctccagcga gttctacaaa
     9841 gacggcaaat accacttgga agccgaaggc cgctcctaca ccaacgcgga atttgccgaa
     9901 tatctggaaa gtctggtcaa cgagttcccc atcatctcca tcgaagacgg catggatgaa
     9961 aacgactggg aaggctggaa actgctgacc gaaaaactgg gcggcaaagt ccaactcgtt
    10021 ggcgacgacc tcttcgttac caacccgaaa atccttgccg aaggcattga aaaaggcgtg
    10081 gcaaacgcac tattggtcaa agtcaaccaa atcggtactt tgagtgaaac cctgaaagcc
    10141 gtcgatctgg caaaacgcaa ccgctacgcc agcgtgatga gccaccgctc cggcgaaacc
    10201 gaagacagca ccattgccga cttggcagtc gccaccaact gtatgcagat caaaaccggt
    10261 tctttgagcc gttccgaccg catggcgaaa tacaaccaac tgctgcgtat cgaggaagaa
    10321 ttggcggaag ccgccgacta ccccggcaaa gccgcattct accaactggg caaataaaaa
    10381 aggttaaggt atgaagtggg taactgtcgt tttatccttc gcacttgtct gttgccaata
    10441 cagcctctgg ttcggcaaag gcagcatcgg acgcaacagc agtctgagag aacagattgc
    10501 cgttcaagaa gaaaaaaacc agacactcgc cctacgcaat cattcccttg ccgccgaagt
    10561 ctatgatttg gaaaacggtc aagaagccat ttcggaaatc gcccgggtag aactgggtta
    10621 tatccaagac ggtgaaacct tttaccgact catcaggcat aaccggtaat accgtcaaaa
    10681 agccgtccga accaatgttc ggacggcttt tatttcaaca aactgtcaga cagcccctca
    10741 tcctcccccg acaaaccgca atccagcctg acatccccct cgacgcaaca gcagcacggc
    10801 agtatctcgt cccgccccaa aaaagccaaa ggcggctccc gataagtaac gcttccctcc
    10861 aaaatcttca ctcggcacga tccgcaatat ccgcttcggc actgatattc caccatatgc
    10921 cccgtccgtt ccaagccttc caacagggtc tcgccctcca agagctcaaa aaaaccctta
    10981 ttcgtaccaa tgcgcgccat ttccgaccaa tcaaaaatat agtggattaa atttaaacca
    11041 gtacggcgtt gcctcgcctt gtccggattt ttgttaatcc actataatcc actataaaag
    11101 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
    11161 aacatccggc ttcagacggc attttttcaa aaaacattta caactcaaaa tcgcccaaat
    11221 catccgtatt cacttcagaa tctatctgac cgatcaaata agaggatatt tccacttcct
    11281 gcggcgcgac ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt
    11341 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
    11401 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca
    11461 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
    11521 cctgcaactc ggcggcaatt tccgccattt cagaatcatc aacaccagaa cgcatcagat
    11581 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
    11641 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
    11701 cgtagaaacg gatggcttcc aacacattga cgcacatcag gcagagatag agttttttct
    11761 tcaacccgcg caaagacacg gtaacgggtt tgccgccgac attgtgcacc ccttcgccca
    11821 acaggttgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
    11881 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
    11941 caatattgcg gatgatgtgg gtatagctgc gcgagtggat ggtttcgctg aagctccacg
    12001 tttcaatcca cgtttccaac tcgggaatcg aaaccaaagg cagcaaagca acattcggac
    12061 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
    12121 tttcgtgttc tggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
    12181 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
    12241 ggtcataacg ggcgacatta accggctgac cgaaaaacat cggctcattc agcgcgtcgt
    12301 ttttggtttt gggaaaggtg ctgtatgaca tagttaagtg ttcgcaggac ataatctatt
    12361 ctctatatta attaaaaatg atctgtaagt tttaactaac ctgctgagac tttatatatt
    12421 taataaaatt ttctaaagtt aaaacatggg ttttcccaaa agttccaaat gtttttacgg
    12481 attctttagg gacaactaag gttaaattct catcactcat ttcctgaatt tgagtatcag
    12541 atataccttc ttgtagagta aataaatgct tatgaggaat tcgatctgct tcatttaaaa
    12601 cttggcgcca gcgatcctta caagtagtct ttgaacctag cattgtcagc ttttcagttt
    12661 gaaatatgaa attccctgat tcatccatcg catggtactc ttcacttcca ggaaataaaa
    12721 aatctggttt ctttttccct tctgtcttcg cttgcgtctc aaattttaag gaaaattcgt
    12781 caaatattct tgataaatgt aactctaaag attttcctgc tcttgctttt ctgcgatttg
    12841 taaaagaatg tgcaaaatta atgaagctat ccaaatcagt aatttttgat ttaataattt
    12901 caaattcacg tttttcaaaa atggaaaata attcatattc ctttgaaacc cattgtttta
    12961 acacattact aacgttagtt ttatttaatg caatattttc ccttgccaaa actgccattt
    13021 cttcagtttt ggggaagtta ggaaatagtg aaaataattt tgttaggtta tcctctactt
    13081 cttgtttttt aggtaaatat aaacttcctg gtaaaatgtt agtttctgcg ataaatattt
    13141 caatatcttc atctgaagat aaaacaaaag catggaataa taaatcttta taacaagctc
    13201 tgcacaatac caataaggaa ccactatatt tatcacttaa aaattcaaaa ttcttgccaa
    13261 aacccgtaat tctcgcctca tttctcgtgc cttggccgta atatttaaaa ttgctattag
    13321 tgacgcttcc atcttgccaa ttaattttga ttgaaatagt tttatttgtt cccttttggc
    13381 aaactacatc aaagaggtcg tctgaaaaat gtttttgaat ataaaatcct gattggtgac
    13441 taccagtagt accaacatca ttgggacgaa tataacgaca gtatactgca attgatttat
    13501 ttgctgtctg tattgctgaa gctactaagt catcggattt atttactact tgtattgtct
    13561 gagctgccaa gtcattcatt tttttaactc atttataatt tgtttagcaa tagcttgaac
    13621 caacggtaca gcaattgaat tgccaaactg cttgtatgca gctgtcttgg atactgcatc
    13681 aataacaaaa tctttaggaa atcccattaa acgcgagcac tccctaggtg tcagcttcct
    13741 aggatttttt cctttctgag ggatgagtat ttcggaacca tctttgtaat atcgtgcaga
    13801 tagagttcgt gatattccat ctaaatcaac taatccaaaa ccaaatccat taccctttgc
    13861 cttatgtttt ttagcgtaat tttgaaggta aagccataag ttatcagaaa gagtaaaaga
    13921 attatctaca tcatcttcca aaatttgctt taattttggt tgtgattctg gtggggaagg
    13981 aaaattgaaa tttatttcct tattaaaata ttgtctatca aaacctacaa taaaaatacg
    14041 ctccctattt tgaggaacat aatattttgc attcataact tgataaaata tctgatagtc
    14101 aagctcttct aaagtccctt taattacttt aaatgtattt cctttgtcat gcgaaacaag
    14161 gtttttcaca ttctctaaaa gaaaaatttt aggtcgatgt tttccaataa tttcagcaac
    14221 atcaaaaaat agagttccct gcgccttatc taagaagcct gtttctcgtc ctaggctttt
    14281 tttctttgaa acaccagcta tagagaatgg ctgacacggg aatcctgctg ttaatacatc
    14341 aaacttactt ggaatagctg ctttggtttc ctttaatgta atatctccat aaggaatatc
    14401 attaaaattt acttggtagg tttgacgggc tttatcatcc cattcactag aaaatacaca
    14461 tcgcccacca acattctcca ttgcaatgcg aaaaccacct attcccgcaa ataaatcaat
    14521 aaaagtaaat ttctcattat ttaaggtaac tatattatct aattgatttt gtattttatt
    14581 tttcatattt tatatttcag atgatttatt aatctaaagg ccatctgaaa actccccctt
    14641 tcatcaaatc ttacaagccc cgcccgcgca gccgtcatcc tgaatatcgg tctgcgtatc
    14701 gtccgcaccg tcgcgggtgt tatggtagta cagggttttg acgccgtatt tgtaggcggt
    14761 cagcaggtct ttgagcattt gtttcataga aactttgccg ccttcgaatt tgcccgggtc
    14821 gtaggcggta ttggcggaaa tcgattgatc gacgaatttt tgcatcacgc cgacaagttt
    14881 caggtagcct tcgttgccgg gaagctgcca cagggtttca taggcatttt tcagggtttc
    14941 aaactccggc acgacttgtt tcaaaatgcc gtctttcgat gctttgaccg ttaccaatcc
    15001 gcgcggcggc tcgatgccgt tggtggcgtt ggcgatttga gagctggttt cagacggcat
    15061 gagcgcggtc agagtagagt tgcgcaggcc gtatttgacg atttcggcac gcaggctttc
    15121 ccagtcgtaa tgcaaaggct cgccgcagac ggcatccaaa tcttttttgt aggtgtcgat
    15181 gggcagtttg ccttgcgaat aaacggtttg gttaaagagc gtacacgcac cgtattcttt
    15241 ggcaaggttt gccgatgctt tgagcaggta atactgtatg gcttcaaagg tacggtgggt
    15301 cagaccgagc gcggagtcgt cgctgtagcg gacaccgttt ttcgccagat aataagcata
    15361 gttaatcacg ccgataccga gcgaacggcg gcccatagtg gaggtacgcg cggcttctac
    15421 cggatatccc tgataatcta aaagtgcatc gagtgcgcgc acagtcaaat cggcaagccc
    15481 ttccaattcg tccaggctgt ttaatgcgcc caagttgaag gcggacaggg tacacagggc
    15541 gatttcgccg ttcggatcgt tgatattgtc cagcggtttg gtcggcaggg cgatttccat
    15601 acacaagttg gactgatgaa caggcgcgac gcgcggatcg aacgggctgt gcgtattgca
    15661 gtgatcgacg ttttgaatgt agatgcgccc ggttccggca cgctcctgca tcagcgtgga
    15721 aaacaggtcg gcagccggaa tgatgcgctt gcggatatca gggtcttgct cgtatttcgt
    15781 atagagccgc tcaaattcgt cttggtcggc aaaaaacgct tcgtacaatc ccggaacctc
    15841 gttgggcgaa aacagcgtaa tgttgccgcc cttaatcagg cgggtgtaca gtaggcggtt
    15901 gatttgcaca ccgtaatcaa gctggcggat acggttgtct tccacgcccc ggttgttttt
    15961 caacaccagc aggctttcgg cttcgatatg ccacaagggg tagaacaagg ttgccgcgcc
    16021 gccgcgcacg ccgccttgcg aacaggattt gaccgccgcc tgaaacattt tgaaaaacgg
    16081 gatgcagccg gtatgccgcg cttcgccgcc ccggatttcg ctgtccaaac cgcggatacg
    16141 tccggcattg atgccgatgc ccgcacgctg ggaaacgtat ttcacaatcg cgctggtagt
    16201 ggcattgatg gaatccaaac tatcgtcgca ttcaatcagc acacagcttg agaactggcg
    16261 cgtaggcgta cgcacgccgc tcataatcgg ggtcggcagc gatactttaa atgtggaaac
    16321 ggcatcgtaa aaccgtttga cgtaatcgag gcgtgtttct ttgggatatt tgctgaaaag
    16381 acacatcgcc accagaacat ataagaactg cggtgtttca taaatctggc gggtaacgcg
    16441 gttctgtacc agatatttgc cttcgagctg tttgacagcg gcataggaaa aggacatatc
    16501 gcgttcgtgg tcgatatagg cgttcagctc gtcaaattct tcgcggctgt aatcctcaag
    16561 gatatgcctg tcgtattttg cggcatcggt aagtttttta acgtggtcgt aaaggtgcgg
    16621 cggctcgtac tcgccgtagg ctattttacg cagatggaaa atcgccaaac gcgcggcaag
    16681 gtattggtag tccggggtat cttccgaaat taaatcggca gcggctttga tgatggtttc
    16741 gtggatgtcg tcggtgcgga tgccgttgta gaactggatg tgcgatttca actcgacctg
    16801 agatacggaa acattttcca atccgtccgc cgcccaagtg acgacacggt gaatcttatc
    16861 caaatcaatg gcttctaatc ttccgtctcg tttggttact ttcaaatcag tcggtgtatt
    16921 catcgcttcc tcttccactc ttgatattca agacacggtc ttttcaaata aattaaggca
    16981 gacaatatag tggatttttg gcattttctc cagtcttgac aacggttgta tttttcagtt
    17041 tcaggcaatg cgcgataaaa cccgcctgtc gtatttttcc tataatattt gttttatctg
    17101 ataattcttt accgataaaa aacgggtaaa ttttttgcct tttgaccggc tccggctaca
    17161 aggcggtgaa ataaggattt tccgacgaaa aaggaaagct tcctgttttc tgcccctgca
    17221 aattgttaaa ttttgcaaag tatgattttg ccgcgccgcc gccgacaaat tccattttct
    17281 taccgattgg aatttattat tgagattaat gtgttatttg aatctgcata tcaaacggca
    17341 agttttgtcg gctgaatgag tctgaaactg ccgacaattt gcccgctcca tccttccatc
    17401 ctatactgga aggaatgaca aactgaaagg atcgtcatgt ctgtcaaaaa gatgcttgcc
    17461 atactgttgt ctgcaatatt gggactggta tcgacaactg ccgctgccgg tacgtcagaa
    17521 cccgcccacc gcgataccaa acatatccgc aaggcaaaca agcagatgct gcaccccgaa
    17581 tgcaggaaat atttggaacg ccgtgccgcg tggtaccgat cgcaaggcaa cgtgcaggaa
    17641 ttgcgcgaaa acaaaaaggc gcgcaaagca ttccactccc tgccttatgc ggaacagaaa
    17701 atccaatgcc gggcggctta tgaggctttc gatgatttcg acggcggcag tttccgccgt
    17761 taatcccata taaaatatgc cgtctgaaca caggttcaga cggcattttc catagataca
    17821 acaaactatc cttacgccat ttcgtttgag gtcctcccgt tgccgcagcc ttaatatagt
    17881 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacgaaacc
    17941 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac
    18001 gctgtactgg tttaaattta atccactata catccacaaa agcgaatcat actgccacat
    18061 tccgacaacc ggtttcagac agcgatatcc gcatcgtcgg cgacaacggc acggatgctt
    18121 tcttcgattt tatcttttaa agcataacgg tcttcgcttt ccgccgcatc cgccacgcaa
    18181 acgaaatcga cttttatcgt caattttttc atagacacga tgcgccacag gcaggtcggc
    18241 aaaccgacat cggcatatga gggacgagcc gtcctttttc ccgtttcgtc ataataacgc
    18301 agcgcgaccg ccaaaacctt tgcccccgca tcgatggcgg attggaacag cgcggctttg
    18361 aacggcaaaa gccccaatcc ggaggaagtc cgcgcttcgg ggaaaaaact gacgttttga
    18421 ccgcgttgca aggtttcgca gacggcgcgg ttaatcggtt cgatgtcgcg ccgcgaattg
    18481 cggttgatga acaccgttcc cgcgttctgc cccatcttgc ccaataccgg ccagcttttg
    18541 atttcctgct tggcgataaa gctgctcgga taaaccgcgc tcatcgcgaa aatatccagc
    18601 caggacacgt ggttggcggc aaccaagaca ccgttcggat gttcgggtgc gggtctgccc
    18661 acctccaatc cgatatccaa agccgccaaa acccccctgc ccaactcgat taccgcccga
    18721 ttgcgcgact cggggcaacc gccgtcaata ccgcgcaggt ttttcccggt tttgaacagc
    18781 cagaccgcca aacggcacaa gcggcgcaga cgtgtaaaaa atgaagcttt atttgaagac
    18841 atgagcaatt ctttcccgat aatcggtgtt tgttgttgaa acgttcggac ggcataccga
    18901 taaaatgccg tctgaaaccg ttttcagtcc tatttctgac agttcgggca ataaaacgtg
    18961 ccgcgctgcc ccaaagtttc tttcacaacc aaaccgccgc actgggggca cggctgattg
    19021 tgccgcccgt acaccgtata ttcctgttgg aagtagccgc ttttgccgtc gctgtccaca
    19081 aaatccctca aggtactgcc gcccgtttca atggcgcgcc gcaacaccgc tttgacggtt
    19141 tcaaccaaaa gcgcgcactc tttctttttc aggcggttgg caggacggta gggcgaaatg
    19201 cccgccctaa acaggctctc gttggcataa atgttgccca caccgaccac gaccgcattg
    19261 tccatcaggg caagtttgac cgcgcgcttc tgcgccttca gccttgcata cagataatcc
    19321 gcacaaaatg cctccgacaa aggctccggc cccagttttt ccaacagcgg atgatgttct
    19381 tcgattccct cataccaaag tatcgcgccg aactttctcg gatcgcggta acgcatgacc
    19441 gtgccgtctg aaaacacaat atcgacgtga tcgtgtctgt ccggcctgcc gatacgtccg
    19501 tccgacggcg taaaaatccg caagctgccc gacatcccca agtgaatcag cagcacgccc
    19561 gtttgaaagc ggataagcag gtatttcgcc ctcctgccgc aggacaacac ctgccggccg
    19621 gacaaaatct cccccaaatc gggattaatc tgccagcgca gcttcaattg gcgcaatacc
    19681 acggcttcca ccgttttccc ttcaatatgc ggcgcgatgc cgcgcaacgt cgtttccact
    19741 tccggcaatt caggcataac ccctcccgac attttttctg acagatgccg tctgaaagac
    19801 ggccgtccct aatccgcaac ccttgccgca cccgccgcaa gggctttgcc gcccaaatac
    19861 ccgtcccacg cgggcagggg cgttacgccc gccgcccttt tgaccaaaac caaagcgtac
    19921 actgcggcac attgcggcca ccaacggtcg cccgcctttt ccataaaccg ccaaaagcgt
    19981 atttgcccga gcgacgaaac cggcggcaga tacaccataa atttcccaaa ttcaatatcg
    20041 aaaccggcat cggcaagccg ccttttcaac tcgggcagcg gcagacaaaa ccgtttttcc
    20101 ggcaggcgtt cgccgtcaaa ccaacggctg aatccccaga gcgaatacgg attgaaaccc
    20161 gtcagcatca agcgtccgca cggtttcaat atccggtgcg cttccgacag gatttgcgaa
    20221 ggaacaccgc cttcaagcgt atgcggaaaa agcagcatat ccgcagaaac atccgccaaa
    20281 gccatattct ccgccgacat cgacatatct cgcggcacac agacaacatc ttcagacagg
    20341 ctcagccacc tgccgcccac ctgaaccgca cacattcccg aaaaacggta tgaatccaga
    20401 taccgcccga agaaatcctg ttccaatttt gcaacatacc gccccatcgc cgtatcttca
    20461 aaccatgcat ccatattgcg tccgtttcaa acagattgcc tgccgatatt ctattccaaa
    20521 caggatttct gtcaaaaaac acatcggccg cccatttccg aatccgcata aagttcctgt
    20581 aaaacttgac gctttttcag tcaaacagta ccatcggacg ataaaatatg tttattccgc
    20641 cgaatataaa tcatgtccaa actcaaaacc atcgctctga ccgcatcagg tctgtccgtt
    20701 tgtccgggtt tcctatacgc ccaaaacacc tcatcacacc aaatcggttt ggcgattatg
    20761 cgcttaaact cttcaatact cgacctgccc ccgacaaaac aatatttcca atccggcagc
    20821 ctgtggagcg agctgcgcca aggcttccgg atgggcgaag tcaatcccga actggtacgc
    20881 cgccacgaaa gcaaattcat cgcaagccgc agctatttca acagggtcat caaccggagt
    20941 agaccctata tgtaccatat cgccaacgaa gtcaaaaaac gcaatatgcc cgccgaagcc
    21001 gccctgcttc ccttcatcga aagcgcgttc gtcaccaaag ccaaatcaca cgtcggcgca
    21061 tcaggattat ggcagtttat gcccgctacc ggcaggcatt acggcctgga aaaaacaccg
    21121 gtttacgacg gcaggcacga cgtttacgcc gccaccgatg ccgcactcaa ctatctgcaa
    21181 tacctctatg gactgttcgg cgactggccg cttgcctttg ccgcctacaa ctggggtgaa
    21241 ggcaacgtcg gacgcgccat caaccgcgcc cgcgcccaag ggctcgaacc gacctacgaa
    21301 aacctgcgta tgcccaacga aacgcgcaac tatgtcccca agctgctcgc cgtgcgcaac
    21361 attattgcca ctccccaatc tttcggcatg aatatcagcg acatagacaa caaaccctat
    21421 tttcaggcag tcgaaccgga tcgtccgctc gacaacgaag ccatcgcccg gcttgccggc
    21481 atcacgcaaa gcgagctgct cgccctaaac cccgcattca acgtccccgc gtttatcccc
    21541 aaaagcaaac gcaaactgct gcttcctgtc gcgtccgtac aaaccttcca aagcaactac
    21601 ctcaacgccg cacccgacag cctgttttca tgggaagtct atacgcctgc cgccaaaacc
    21661 agcctgtccg acatctcgac ggcaaccggc atgagcattg ccgacatcaa acgcctcaac
    21721 aacctgaacg gcaaccttgt caacgcagga cgcagcatcc ttgtcgccaa gaacggcaaa
    21781 acccttcaga cggcatcgga atccgtcgtt tccatcgaca tcgacaatac acccgacacc
    21841 taccgttcca atatgccggc aggcacggtg aacgtcggca ttgcccgaat ccgacccgcc
    21901 gccgcacaga cagcggacat taccgtcgca cctttgccgc agaaaaccgt ccgtacggaa
    21961 cccgatcccc ttgtccgtat tgccgaacct gcccttgcga cagccgcagc gcaacctcaa
    22021 accgaaaaac agaccgccat gccgtctgaa acccaaaccg caacactcgc gcagatcatc
    22081 ccccaaaacg acatgcaggc ggcagacgaa ctcatgcagc ttgttgcccg aaacaacctg
    22141 cgccgacagg ctgaagaaac catctccgcc gtcatcggca cgcctgacac agttgccgaa
    22201 cacaacattt cctcatctcc gcaacatacc gttgccgccg acggcaaacg ccgggcacgt
    22261 ttggaaacgc gcgtagccaa agctgccgac ggcgaagccg aaatctcccc gctccatgcc
    22321 agcatccacc gcgttgtaga aggcgacacc ctgttcaaca ttgccaaacg ctacaacgtc
    22381 agcgtagccg acctgattgt cgccaacaac atcaaaggca acaccatcca aaaaggacag
    22441 gtactccgcc tggcgcaggc agcccctgcc caaacccgta ttgaaaaagt atcctacacc
    22501 gcgcgcaaag gcgacacctt caaaagtatc gccgcgcgct tcaatatcca tatcgacgac
    22561 atccgccggc tcaatcccaa cctgaacacc atcaatccgg gacagagggt caaactgatt
    22621 ggaagctgat tcggatacgg cacatgacag gactttctca gtcctgtctt tttcatccca
    22681 catttcccat accatcatga aactcatcaa atacctgcaa tatcaaggca taggaagccg
    22741 caagcagtgc caatggctga ttgccggcgg ttatgttttc atcaacggaa cctgtatgga
    22801 cgacaccgat gcagacatcg attcctcatc tgtcgaaacg ttggatattg acggggaagc
    22861 agtaaccgtc gttcccgaac cctatttcta catcctgctc aacaagcccg aagattacga
    22921 aacttcgcac aaaccgaaac actaccgcag cgtattcagc ctgttccccg acaatatgcg
    22981 gaacatcgat atgcaggcgg tcggcaggct ggatgcggat acgaccggcg tattgctgat
    23041 taccaacgac ggcaaactga accacaacct gacttcgccg agcagaaaaa ttcccaagct
    23101 atacgaagta acgctcaaac accccacagg agaaacgctc tgcgaaaccc tgaaaaacgg
    23161 tgtcctgctc cacgacgaaa acgaaacggt ttgcgccgcc gatgccgtgt tggaaagccc
    23221 gtccgcattg ctcctgatca ttaccgaagg caaataccac caagtcaaac ggatggtcgc
    23281 cgccgcaggc aaccgcgtgc agcatcttca ccgccggcga ttcgcacatt tggaaacaga
    23341 aaacctgaaa cccggcgaat ggaaatttat cgattgtccg aaactctaaa ataacatcca
    23401 agaattccat ttatattcat ccacatactc attaaatata tggtttaacc caatttaacg
    23461 caaaaaatca atttacgata taatccattt gtctgagtaa cgcctgttca agcaggctta
    23521 tgagtaagac gttttccccg taatgtgttt tgccatctat acttctcccc ttgtatagat
    23581 ggttttttat agtggattaa atttaaacca gtacagcctt gcctcgcctt gccgtactat
    23641 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatttcaaa
    23701 ttccctctct ttcatcatta tttaaatgct atcattaata taattaacaa aatttcattt
    23761 ccatacaccg atgtagaaga ataacaaatt aatctattta taaactccga ttcttcctca
    23821 tcgggttata ctcatcagaa atactcactg aaatatatgc cttttacatg gaaaatcact
    23881 agacagttga aaaacaatta gtcagttcct gttaaaaaac gcgtgtagaa tttccgcttt
    23941 atctcaatcg gacacggttt caatatgtct tccccttcaa ataccaatcg tcaaacctga
    24001 tccagccgat taacctatat cctgaccgtt gccggcgcga ctgtcggctt cggcgcgacg
    24061 tggcgttttc cctaccttgt cggcgaaaac ggcggcggcg cgtatgtgtt tttattctgt
    24121 atcgcgatgc tggttatcgg catcccgatg attttggtgg aaaatgtcat cggacggcgc
    24181 aaaggcgtga acgcgctgga tgcgttcggc ggcccgatga acggcaaacc cattgccaaa
    24241 atttggaaac tggtcggctg gatgggctgc tcggcgcgtt cggcatcatg gcttattaca
    24301 tggtgctcgg cggctgggtt atcagctaca ttgttaatat catcggcggt aatttggaca
    24361 tttccagtcc tgtttccagc gaagtcacca aaagtttctt taccgaacac atcgaaaaca
    24421 gtccttggga aattgctttc tacaccttcc tatttgtggt tgtgaaccaa tggatcttgg
    24481 taaaaggcgt gatcggcggt attgaaaaag cggcaaaata cctgatgcct ttgctgtttt
    24541 tattcctgat tgccatggtt gtgcgcaacg ttaccctgcc gggcgcaatg gaaggcatta
    24601 ccttctatct gaaacctgac ttcagcaaaa ttacagccga actgttcgtc ttcgttttag
    24661 gacaagtgtt ctttaccttg agcttgggtt tcggcgtgat gattaccttg tccagctatc
    24721 tggataagaa cgaaaatctg gtgcaaaccg cggtcatcac ggccattacc aataccatca
    24781 tcgccgtact ggccggcttt atgattttcc cgtcgctctt cagcttcggc gtcgcgccca
    24841 attctggccc gactttggta ttccaaagtt tgccgattgt gttctcacat atgtgggcag
    24901 gacctgtgtt cgctgtcatt ttcttctctc tgctcctgat tgccgcgctg acaacttcgc
    24961 tgacgattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
    25021 ccgcaatcac gattgtattg tccgccatct tcatcttcgg caatattccg tccattctga
    25081 gctatggccc gtggaaagat gtttccatat ttggcaaaaa tatttttgac gcttttgact
    25141 acatcagcgg caacatcttg tttatgctga ccgcgctcgg ctccgcgctg tttgtcggtt
    25201 ttgtgatgaa ggatgaagcg aaggacgaat tgctttataa aggcaaccat ataacggtca
    25261 atatttggtt tgcctatgtg aaataccttg tgccgctggt gattttgctg attttcgtca
    25321 gcaacctgtt ctaatccgca gcaatcgatg ccgtctgaaa gtcatacctc tttcagacgg
    25381 cattgcgttg atgcccgcca caatacccgc aacgccagaa tccgaaccgg cttgccggct
    25441 tctgtcggat atttccgggc aggcggcgtt ccgtctgctt agacaatcgt cctttaaaac
    25501 aggtagaatc tgccccaacg ggaaacacat ccttcagacg gcaaaaccca taccccaaac
    25561 catcaggaat cccccttatg aacagacaaa aagtcatcgc catcgacggc ccgggcgcat
    25621 cgggcaaagg cacggttgcc gcccgcgttg ccgccgcatt gggatacgat tatctcgata
    25681 ccggcgcact ctaccgcctg accgccctat atgcacaaaa acaaggcgtg gaatggcacg
    25741 atgaagaaaa cgtttccaaa ctggcaaaaa aactgcccgc cgtattttca ggcagccgca
    25801 tcctgctcga cggcgaagac gtttcagacg gcatccggac agaagccatc ggcatgggcg
    25861 catccgcagt cgcacagttg cctaaagtcc gcgcctccct gctgcaacgc caacgcgatt
    25921 ttctgaccga aaaaggactg gtcgccgacg gacgggacac cggatcggtc gtcttccccc
    25981 aggccgaact caaaattttt ctgacagccg aatccaaaat cagggccgaa cgccgcgcca
    26041 aacaaatcgg catcccctgc gaaggtttgg cattcgagcg catcctgtcc gacatcgaag
    26101 ccagagacga ggcagaccga aaccgcaagg ttgcccccct gaaacaacaa cccgatgccc
    26161 tgcttttgga tacgagccgc ctgactatag aagaaactgt aaaaaaagtg cttgattggt
    26221 atcgtaaagt ttaaattttc aggtataatc gcccgcattg cgtttcagac agcatgaatt
    26281 tccccatatg ccgtctgaaa ccttatttac ccgtctgccc tgccaaggac ggcagacacc
    26341 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
    26401 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
    26461 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
    26521 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
    26581 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
    26641 ctgtcccgcg aaaaagccaa acgcgctgcc gattggattg ccctggaaga agccatggaa
    26701 aacggcaaca tcctgtccgg catcatcaac ggcaaagtca aaggcggcct gaccgttatg
    26761 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
    26821 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
    26881 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
    26941 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
    27001 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
    27061 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
    27121 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
    27181 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
    27241 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
    27301 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
    27361 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
    27421 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
    27481 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
    27541 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
    27601 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
    27661 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
    27721 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgtcgatgcg
    27781 aaaggcgccg ttgtcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
    27841 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
    27901 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
    27961 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
    28021 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
    28081 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
    28141 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
    28201 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
    28261 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
    28321 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
    28381 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
    28441 gtggtttaac ttcatccgct gcttcaatac cttgagccaa gcaagcaacg gattagagcg
    28501 tggatttttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
    28561 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
    28621 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatcatt gcttacaatc
    28681 cgctttttaa acaacaaatt tttgatttct attacgaaca ggacaaaaat cctgcttatt
    28741 gcactaaaac taagccgttt caggaatttg cggcaaattt acagctttta ccgagcctaa
    28801 tgctttcgct ttttggtaaa acgccaattt gtattcaagc aaatctaaat agcgttttaa
    28861 ttcggcaatt tgacacttca cattttctat ttgattttca aacaaggaaa ggcgttcttc
    28921 aatggtatcg tcgccaatga cggtacattc cgcaaagcgt ttgatgtctt ttaagctcat
    28981 tcccgtattt ttcaagcatt gcaataagcc caaccattgc aaatcgttat cggtaaaaca
    29041 gcggttaccg tattcatcac gtccgatatt aggcaacaaa ccttctttgt cgtaaaaacg
    29101 tagggtgtgg gcggagatgc ctattttttc ggcggctttg gcagtggtat aagtcatttt
    29161 ccctccttct aaacaacaac
//
LOCUS       DAXIHS010000017        28840 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXIHS010000017 DAXIHS010000000
VERSION     DAXIHS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.017"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(61..1317)
                     /locus_tag="ACFOF2_000646"
     CDS             complement(61..1317)
                     /locus_tag="ACFOF2_000646"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC7107005.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLFDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1665..2006)
                     /locus_tag="ACFOF2_000647"
     CDS             complement(1665..2006)
                     /locus_tag="ACFOF2_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107006.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2209..3066)
                     /locus_tag="ACFOF2_000648"
     CDS             complement(2209..3066)
                     /locus_tag="ACFOF2_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC7107007.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFTNAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFHAFEPPLNPYGWRDVYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3127..3942)
                     /locus_tag="ACFOF2_000649"
     CDS             complement(3127..3942)
                     /locus_tag="ACFOF2_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC7107008.1"
                     /translation="MTTLKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4006..4176
                     /locus_tag="ACFOF2_000650"
     CDS             4006..4176
                     /locus_tag="ACFOF2_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107009.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            4186..4971
                     /locus_tag="ACFOF2_000651"
     CDS             4186..4971
                     /locus_tag="ACFOF2_000651"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC7107010.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            complement(4952..5170)
                     /locus_tag="ACFOF2_000652"
     CDS             complement(4952..5170)
                     /locus_tag="ACFOF2_000652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107011.1"
                     /translation="MLIHYKQIVQGSPFNQIWVSIFGSFGHLAVSTFLPPLPVKMPSE
                     ISARYFRRHTRPFSRIPGSGLKFKRVRK"
     gene            5241..7336
                     /locus_tag="ACFOF2_000653"
                     /pseudo
     CDS             5241..7336
                     /locus_tag="ACFOF2_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            7460..9010
                     /locus_tag="ACFOF2_000654"
     CDS             7460..9010
                     /locus_tag="ACFOF2_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC7107012.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLITAVKGEASGLFTHNPVLNIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     GMVSEPQQ"
     gene            complement(9370..11157)
                     /gene="lpdA"
                     /locus_tag="ACFOF2_000655"
     CDS             complement(9370..11157)
                     /gene="lpdA"
                     /locus_tag="ACFOF2_000655"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC7107013.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(11234..12817)
                     /gene="aceF"
                     /locus_tag="ACFOF2_000656"
     CDS             complement(11234..12817)
                     /gene="aceF"
                     /locus_tag="ACFOF2_000656"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC7107014.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAAPVAAGGATVQV
                     AVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVG
                     DKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPSAPAAAKID
                     EAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAA
                     AGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEAD
                     MTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFN
                     IGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSL
                     GGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAG
                     MRFTVFLAKLLKDFRRITL"
     gene            complement(12967..15630)
                     /gene="aceE"
                     /locus_tag="ACFOF2_000657"
     CDS             complement(12967..15630)
                     /gene="aceE"
                     /locus_tag="ACFOF2_000657"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC7107015.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            16324..18036
                     /locus_tag="ACFOF2_000658"
     CDS             16324..18036
                     /locus_tag="ACFOF2_000658"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC7107016.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVVRVTA
                     LLNG"
     gene            18099..18755
                     /locus_tag="ACFOF2_000659"
     CDS             18099..18755
                     /locus_tag="ACFOF2_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC7107017.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(18765..19220)
                     /gene="ruvX"
                     /locus_tag="ACFOF2_000660"
     CDS             complement(18765..19220)
                     /gene="ruvX"
                     /locus_tag="ACFOF2_000660"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC7107018.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(19213..19761)
                     /locus_tag="ACFOF2_000661"
     CDS             complement(19213..19761)
                     /locus_tag="ACFOF2_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC7107019.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(19763..20323)
                     /locus_tag="ACFOF2_000662"
     CDS             complement(19763..20323)
                     /locus_tag="ACFOF2_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC7107020.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            20651..22441
                     /locus_tag="ACFOF2_000663"
     CDS             20651..22441
                     /locus_tag="ACFOF2_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC7107021.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            22568..24052
                     /locus_tag="ACFOF2_000664"
     CDS             22568..24052
                     /locus_tag="ACFOF2_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC7107022.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            24381..26408
                     /gene="uvrB"
                     /locus_tag="ACFOF2_000665"
     CDS             24381..26408
                     /gene="uvrB"
                     /locus_tag="ACFOF2_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC7107023.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIAQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVMRDRIRNIKESLLFGAE"
     gene            26454..27722
                     /locus_tag="ACFOF2_000666"
     CDS             26454..27722
                     /locus_tag="ACFOF2_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HFC7107024.1"
                     /translation="MKLSKTRIDKSGLALAKQQYKDEIDFIELEECFDLYRQSHLSPL
                     SKTTIDIQNILREYIGEYYIAQRLKRKPQIIRKLNRLSVRLTQLQDIGGCRIIVPTNR
                     DVDKIYKFLSSKKDNLDFLIKKVTDYREKGRDDSGYRALHIIIERENLNFELQIRSRI
                     QHYWAESIEKTSVVYGYYLKEQEGDRDVINYFKELSDVFYEIESGRKPTTIQKLNLED
                     LRKRCEVIIQKSDTHKILNSYVNEGIISALIEKEKRNTNSLNNWILIFDWNAGVFVSW
                     DVVAQDPKQAVEVYVNYENQYPSLEGFEVVLIGSSDIASVQKTHSHYFGIASYENILE
                     TLDESIFCFAKKDDIDIGARRILAAMVRHKFWGTKTVTSETLKNHFCQNLFGFDYSLR
                     DLIRKELIKYGRDKDLSLNIAKKMEIESYL"
     gene            28132..28617
                     /locus_tag="ACFOF2_000667"
     CDS             28132..28617
                     /locus_tag="ACFOF2_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC7107025.1"
                     /translation="MPSFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRERY
                     PFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSKYLIGGNLRLWQKPFWEHTI
                     RDEADFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIGY
                     D"
     gene            28654..28836
                     /locus_tag="ACFOF2_000668"
                     /pseudo
     CDS             28654..28836
                     /locus_tag="ACFOF2_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         7283 a         6991 c         7664 g         6902 t
ORIGIN      
        1 tgatgaaatg ccgtctgaac gctttgaaaa gggttcagac ggcattttgt ttaaacaggg
       61 ttatttgcgt tgcaatacgg cggcgaagaa gccgtcggtt tggtgtcggg cggagttgag
      121 gcgcaggtat ttgccggtat ccaaatcgat tttcaaaccg gcaagcagtt cggcgcagtt
      181 gacgggttca aattcgggat gttcggacag gaaacgttcg acttgcagct cgttttcttc
      241 gggcaggatg ctgcaagtgg cgtaaaccaa gcggccttgc ggttttacca gtttggaggc
      301 ggcatcgagg atgctgtgtt gctgttccaa aaggttggcc acggtttcgg cggattggcg
      361 gtatttgagg tcgggattgc ggcgtaaagt acccaaaccg gagcagggcg cgtccaccaa
      421 cacacggtcg gctttgcctg ccagtcgggc gatacggctg tcgtgttcgc tgctgatgcg
      481 ttcggggtgg atattggtca gtccggcgcg ggtcatacgc ggtttgaggt tggcaaggcg
      541 tttttcggcg atgtcgaagg cgtagattct gcctttattg gccatttgcg caccgacagc
      601 caaggttttg ccgccggcac cggcacagaa atcgacaatg atttcgccgc gttttgcgcc
      661 caccaataaa gcaagcagct ggctgccttc gtcttggact tccagtgtgc cgtctaaaaa
      721 cagttcgtgt ttgttaagcg cgattttgtt tttcaggcgg atgccccaag gcgaataagg
      781 cgttgcctct gcatcggcac tttcggcttg caacagcggc agcactttat cgcgtttgcc
      841 tttcaaagtg ttgacgcgga tgtcgagcgg ggcaggctgg ttgatgctgc ggccgaaggc
      901 gaggatttct tcttcacgcc aatgctgttg cagttgttcg accagccatt gcggcaattc
      961 tgcggcggta ttcaggccgt ctgaaaactc ggttttacgg gctttcaaat tgccgaggaa
     1021 cgctgtttct tcttcatcaa acaggtcttt gatttggctg atgttggtgc ttctgccgag
     1081 aaccagtgcg gcgagtgcgg ctttgcgcgg ctgtgcgtgc ggacggcgca gggcagtgct
     1141 gattttttga tagtggcgca gcgcggcaaa ggcggtttcg gcgatttcgt ggcggtcttg
     1201 gcggccgagc tttttatgtt cgcggaaata ggcggagagg acggcatcgg caggctgttt
     1261 gaaagtcagc atttcagcca aaactttggc ggtatggtcg agttgtgcgg cgttcataat
     1321 gtaatgggtt ctcttactgt gggttattca taatcaatct aacgtcctta aaataacaat
     1381 agcatcttta tcattactta gtattctatc cgcaataaga aagatgtgtt aaaagtgcgc
     1441 aatcgggatt tgtctaaatt tctccgttaa ctacaaaggc agcctgaaaa aagttctatt
     1501 ttcaggtcgc ttgaacagca tccgatataa tcacatcagc gatttcattt tgtaatcact
     1561 ccccaaaaca tgaggaaaaa aacaaaattt attttcttcg ttcagtatat ctatcctaaa
     1621 tatgccatca ctccattgag tttttaattt gtatttcgct cttgttaaat gtttttctgt
     1681 ttgccgattt cttttgctaa tgtaaaaaat actctaccac tctcattggt taaagttttc
     1741 aagtattcat caacatcagt aacattaata acaactgttt catttgtatg tttattaaat
     1801 tctaaatcaa taataatatt cccattaacc aatatttcat atatatcttc ttcttcaggg
     1861 agcatagaca aacaataagc acttgaagct gaaaactctt ttttcagata ctgaagaaga
     1921 aaagtatttt taattaaaat attattaaat ttatctaatg attgcttgac ttctttagtt
     1981 gtccaatttt taacattatt cttcattgta ttctacctgg gtcgtagtta ttaaattacc
     2041 atacttattt gtttggatcg tcataatatt agttggcatg ttgttgttat atttatcata
     2101 tccaatatcc ttattaactc ggatgactct ttcataaatt gtatgggaaa tgagatttcc
     2161 atcactaagc ctctcaatag atatacgcgt taatgttggg aaagggtttc agacggcctt
     2221 cataatgcgt tcgatttcgt gccaaaagcc gtccggccga cattccaaaa cggcaatcaa
     2281 gccttggtcg atacggcgga tttcggtttc gttcaatgtt tcggtttcgg cggcacgcgc
     2341 aggtgcgagg taggccatgc ggtcgagcag atggtagcgg acttcttggc gttcaaaccc
     2401 gtattccgcc caatcttgaa tatagacgtc gcgccagccg tatggattaa gcggcggctc
     2461 aaaagcatgg aaaccttgcg gaaaaaatcc gatgccgcgc acgatggaaa cgggttttac
     2521 gtcaagcggc agaccgtgtt gccgcaaggt tcggatgccg tctgaagtgt gcggcagacc
     2581 gagttgggcg gtcagtttgg cggcttttcc caacagcgtg tctttggggt cgagtccgcg
     2641 catgtcttcg agcctgcccg tgccgccgcc gtaatatttg caggtcagct cgatatggta
     2701 gggcttgccg ttaagcctta ccacaaaatc cgccgcgcct tgcgtattgc cgtccgaacc
     2761 ggaaaccgtg aggttgtgcg cgagcagttc ggcgtgtggc gcgttggtaa accaaaatgc
     2821 cagcagttct tcggcgtaaa tgccgaggcg gtgtccgaac ggggcgcgtc gggtgaggtg
     2881 ttccgtcagc ggcgcgggat cggcatccaa tgccaaaaga tagcggaaac cgcgttcccc
     2941 caatagttcg cgcacgccca attcgcaacc gctttgccac aaaggcggcg cagtcagcag
     3001 cgaggcgagg tcgcggacgg gttggctggt gagcttccac cataatgcgt ctagggcgta
     3061 attcatgctt atccgaatat cagtcatgcc gtctgaaaag ctggaaatgt tttcagacgg
     3121 ccttaatcag tcttttttat ggatacgatg gctgacggta ttgaaaaaac cgcgcaggat
     3181 atcgatgtct tcggtttgcg tgttggcgcg tccgaacagg ctctgcatac ggcgcatcag
     3241 acgctcgccg ttgcggcggt tgaaaaagcc gatgtcgttc atcacgcttt ccatgtgggc
     3301 gaccatgcct ttgatttgct cgtgggtcgc agcgtggtct tcttgttgaa gatgggtcat
     3361 gggcgaaccg gtttggctga agatttcgta gcacacgacc tgcacggctt gggcgaggtt
     3421 gagcgagaaa tagtcgggat tgccgttgat ggtcatcagt cggttgcagg cttggacttc
     3481 ttcgatgctc aagccgaaag tctcgttgcc gaaaaccagt gccactttct cgcctcggtt
     3541 tgcggtctgc agtaattcgg ataccaaatc gcgcggggtt tgcagcggcg cagtaatttc
     3601 gcggcggcgg ctggtcaggg cgcaggcgat ggtggtgtcg gcaagggctt cgtccaaaga
     3661 agcggcaatg gtggcatttt ccaaaacgtc tgccgcgccg gaagcgagga tgaagctttc
     3721 ttccggtaat ttaaacgatt gaggatgctc cgggtcaaac acgggcgggt tttccgtcat
     3781 cggcgttgcc atcagatttg gggcgacgat ggtcagtttg tgcagaccca ttgttttcat
     3841 cgcgcgcgcg gcagagccga tgttggcggg atggctggtg cgcgtgagga taatgcggat
     3901 gttgccgaga tagtcgggca gggcgggttt gagagtagtc atgttcggtt ttcggtcgga
     3961 atggaatagg gtataatgcg ccgtcttttt cgttcggacg gtcaaatgcc gtctgaaatg
     4021 ttctttaaca acgtcggaaa tttaacgttg cgcctgaagc agacacgcgt cgggcggtat
     4081 tttacccgat ttggcaggat ttaccccaac caaataaact ccgattttgc cgctccgaag
     4141 gactgcacgt ccgaaccggc ggtatgccac ccttaaggaa atctgatgaa tccgtttttg
     4201 aatacagcct ttaaagccgc ccgccgtgcc ggtcagatga tgatacgcgc cgcgggcaat
     4261 ctcgatgccg tcaaaaccga cagcaaagca ttcaacgatt ttgtttccga tgttgaccgc
     4321 aattccgaaa tcatcctggt tgaggctttg aaagaagcct atccgcacca caaaatcact
     4381 tgcgaagaaa gcggctccca cggtaaagcc gcagccgaat acgaatggat tatcgatccg
     4441 ctcgacggca cgaccaactt ccttcacggc catccccaat acgccatctc tatggcactc
     4501 ctgcacaaag gcgtgttgca agaagctttg gtgtacgccc ccgaacgcaa cgacgtttac
     4561 atggcttcgc gcggcaaagg cgcgttgctc aatgaccgcc gcatccgcgt ttccaaccgc
     4621 atcgaattga accgctgcct gatcggtacc ggctttcctg ttgtcgatca aagcatgatg
     4681 gacaagtatc tggcgatttt gaaagatttc ttggcaaaaa ccgccggcgg ccgtcgtgaa
     4741 ggcgcggctt ctttggattt gtgtgctgtg gcgactggtc gttttgacgg ctttttcgag
     4801 ttcaacctca aaccttggga tattgccgca ggcgcattga ttgttcagga agcgggcggt
     4861 atcgtaaccg atatgacggg tgaagacggc tggctggaaa gcggcgatgt tgtcgctgcc
     4921 aatcccaaag tactggcgca aatgttgaaa atcatttccg cacacgttta aatttcagcc
     4981 cgctcccggg aatgcgggaa aacgggcggg tatgccgtct gaagtatcgc gccgatattt
     5041 cagacggcat ttttactggc agcgggggca ggaaggtgga aacggctaaa tgcccgaaac
     5101 tgccgaagat ggaaacccat atctgattaa acggagaacc ctgtacgatt tgtttatagt
     5161 ggattaacaa aaaccagtac ggtgttgcct cgccttagct caaagagaac gattctctaa
     5221 ggtgctgaag caccaagtga atgggcaaag ccaacgcctt taccgcaccg attaccgtcg
     5281 tcaattacga cgaacaagcc ctcaataaca ccgaagcgcg tactttggtg gatgccgtag
     5341 cgaaaaaaga cgcttccgtt tggcagttcg gcggcgaaag caacacgctg accggtctgt
     5401 atttcagagg ttatcagctt gactcgcgcc aattcagcgt caacggtttg gcaggtatgt
     5461 acggcacgca aggcacggcc agcgtgcaag tcggctcggc acaactgatt aaaggcgcgt
     5521 ccaccgccgt aaacggcatg gaccctgaag gcgcggtatc cggttccgtc aatattgaaa
     5581 ccaaaaaagc cgccgacgaa gacaaccgca aaatcggttt gggctggttt agcaacaacc
     5641 gcgcccaagg tacgttcgac ttgggtcaac gcttcggcga aaacaaagaa ttcggcgtgc
     5701 gcgccaacgg caaactgcgc catggcgaca ccccgcgcca cggttacagc gaagacaaca
     5761 aagaatttgc attgaatgct gattatcgcg gcgaaaaagt gcgcgtggcg ttcgactcca
     5821 tctacgcgaa acgcaaaacc aacggcggcc gcgcgcgtat gcaggatatt caaaacgccg
     5881 gcggccgctt gtttgacgcg ccagacggca aaatcaacct gctgctttcg tggaactggc
     5941 aaaacaccgt cggcgaaacc aatatgctga cctttgaatg ggatgcgttt gacaatgccc
     6001 aaattaccgg cggtatcggc tacaacaagg cgcgttatta cggcacactg atttccccga
     6061 ccgtttgcgg tacaagcggt gcgagcgacc aaaccgctac ctgcacaagt gccaagcaat
     6121 accacaccgg cacggcgagc ctgaccgacc aatatttccg cacattgagt atgaacctga
     6181 ccgcacgcgg cgaatttgaa accggcccgg taacacataa ctggagtatg gctttcgacc
     6241 gcgttatccg ccagcggaaa accataaacg gcagcgggaa cggcaacagc aagatagaag
     6301 tgaaggcaaa cgaaaatatt gcgaatcaat tggcatcgtt tacagccgac tatcccaatt
     6361 cttgggagaa ctctgccaat ttggatgcca atatcaaagt caacagcctg gctttgtccg
     6421 acaccttggg cttcgtggat aacaaatacc gcctgacttt gggcggacgt ttccaaacag
     6481 tcgaatatac cgacaaaaaa tcacagagcg gcgatgccaa acgtttcagc ccgatgctga
     6541 tggcggcgtg ggtgccgcaa cctgatttgg ttgtgtacgg caactatatg gaagacttgg
     6601 agcctgccga catcaaaacc gatgattccg gcgaaacgac aatggcgaaa ccgcgtgtca
     6661 gccgccagtt tgaagtgggt gtgcgtaaaa actggggcaa tttcgtaacc acgttaaacg
     6721 cgttccaaat caaacgcccg gctactggcg cggtcataca actaccaaga caacaaaagg
     6781 ggtaacaaca acgctgacct atggcaacaa ttccgatttc gcccgctata aggcgcaagg
     6841 cggcgtagcc ggcgacgagc aaggtatgga acgcaatcgc ggtatcgagt tcaatgccta
     6901 tgctaacctg ttaaacaaaa ccctgcgtcc gactttgggc ctgatgtatc tgcaatcgac
     6961 cgtgaaagac tacccaaatt cacgcgatat gctggttaac ggcgtacaag tcgccaatcc
     7021 gcgcgtaatt gccaaggcag gtttggaatg ggatacaccg tttgccaaag gcttgacttt
     7081 aaacagcaat gtttcgtatt tcggcaagtc ttaccaagac acgcaaaaac aatacgcctt
     7141 cccatcctat accttggttg atgtaggcgc gcgctacaaa accaagctcg gcaaaaatac
     7201 gttgaccgtc agcagctcgg tggaaaatct gttcaacaaa aactactggc aggtacagcg
     7261 cggccaatac gaccgcagct tcgccgtcgt cggtctgccg cgtacttact ggctgaaggc
     7321 ggaattggat ttctgatttc tccgcatctg gaaaatgccg tccgaacagg tttcagacgg
     7381 catttctttt aaagagatat ggcgggatac gcttaaataa tgctattcta gcgtctttga
     7441 aacctgcttg tttgaagtga tgaaaaaacc tttgatttcg gttgcggcag cattgctcgg
     7501 cgttgctttg ggcacgcctt attatttggg tgtcaaagcc gaagaaagct tgacgcagca
     7561 gcaaaaaata ttgcaggaaa cgggcttctt gaccgtcgaa tcgcaccaat atgagcgcgg
     7621 ctggtttacc tctatggaaa cgacggtcat ccgtctgaaa cccgagttgc tgaataatgc
     7681 ccgaaaatac ctgccggata acctgaaaac agtgttggaa cagccggtta cgctggttaa
     7741 ccatatcacg cacggccctt ttgccggcgg atttggcacg caggcgcaca ttgaaaccga
     7801 gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc ttttttggga aacaagctcc
     7861 ggtttccctt gccaataccg tttattttaa cggcagcggt aaaatggaag tcagtgttcc
     7921 cgccttcgat tatgaagagc tgtcgggcat caggctgcat tgggaaggat tgacgggaaa
     7981 aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc tatgatgcgc ccttgttcaa
     8041 aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa gcgcatttcg attcggaaac
     8101 ttcagacggt atcaatccgc ttgctttggg cagcagtaat ctgaccttgg aaaaattctc
     8161 cttagaatgg aaagagggtg tcgattacaa cgtcaaattg aacgaactgg tcaatctcgt
     8221 taccgatttg cagattggcg cgtttatcaa tcccaacggc agcatcgcac cttccaaaat
     8281 cgaagtcggc aaactggctt tttcaaccaa gaccggggaa tcaggatcat ttatcaacag
     8341 tgaagggcag ttccgttttg acacactggt ttacggcgat gaaaaatatg gcccgctgga
     8401 tatccatatc gctgccgaac atctcgatgc ttctgcctta accgtattga aacgcaagtt
     8461 tgcacaaatt tctgccaaaa aaatgactga agaacaaatc cgcaatgatt tgattactgc
     8521 agtcaaaggc gaggcttccg gattatttac ccataacccg gtgctaaata tcaaaacttt
     8581 ccgtttcacg ctgccgtcgg gaaaaatcga tgtgggcgga aaaatcatgt ttaaagacat
     8641 gaagaaggaa gatttgaacc aattgggact gatgttgaag aaaaccgagg cagacatcag
     8701 aatgagtatt cctcaaaaaa tgttggaaga tttggcggta agtcaggccg gaaatatttt
     8761 cagtgtaaat gccgaagatg aggcggaagc cagagcaagc attgcagata ttaatgaaac
     8821 attgcgcctg atggtggaca gtacggttca aagtatggca agggaaaaat atcttacttt
     8881 agacggtaat cagattgata cggttatttc ccttaaaaac aacgccctga agttaaacgg
     8941 gaaaacgctg caaaatgaac ctgatcccga ttttgatgag ggcggtatgg tttcagagcc
     9001 gcagcagtaa tgggtcaaat gccgtctgaa atgttcaggc ggcattttgt tgtattggaa
     9061 actgccgttt tcaaccattc ctttagataa gccgtgtcgg attcgttttt atagtggatt
     9121 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
     9181 tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc gttgccttgt
     9241 cctgattttt gttaatccac tataggtcgg agaagatatt ttaatccgct gtaaaataga
     9301 aaatgccgtc tgaacatttc agacggcatt tgctcaaata aaccttatcg gctgtttatt
     9361 cagtcggatt tattttttct tttgcggagg caggtcggta caagtaccca atgccacttc
     9421 cgccgccata ccgatggatt cgcccaaggt cgggtgcggg tggatggttt tgccgatgtc
     9481 tgccgcgtcg cagcccattt cgatggcaag gcagacttcg ccgatcatgt cgccaccgtt
     9541 cggaccgaca atgccgccgc cgatgatgcg gccggtttcg gcatcaaaaa tcagcttggt
     9601 aaagccgttg tcgcaaccgt tggcaatcgc acggccggaa gccgcccacg ggaagttggc
     9661 tttggtgatt ttgcggccgg atgctttggc ggacaactcg gtttcgccca cccacgccac
     9721 ttcgggggaa gtgtaggcaa cgcccggaat cacgcgcgcg tcgaagtagg ctttgtggcc
     9781 ggcgcagttt tcagcagcaa cgtggccttc gtgaacggct ttgtgtgcca acattggttg
     9841 accgacgatg tcgccgatgg cgtagatgtg cggcacattg gtacgcattt gtttgtccac
     9901 ttcgatgaag ccgcgatcgg taacggcaac gcctgctttt tccgcgctga tgagtttgcc
     9961 gttgggcgcg cggccggcgg caaccaatac ggcatcgtaa cgttgcggct ctttaggcgc
    10021 atttgcacct tcaaaggtaa cgtaaacgcc gtcttctttc ggctcgactg caacggtttt
    10081 ggtgttgacc ataatgttgt caaaacggta ttcgttttgt ttttgccata ctttcaccaa
    10141 gtcgcggtct gcgccttgca tcaggccgtc catcatttca accacatcca aacgcgaacc
    10201 cagcgtgctg taaaccgtac ccatctcgag gccgataatg ccgccgccga taatcagcag
    10261 tttgcccggt acttctttca gagccaatgc gccgctggaa tcgatgatgc gcggatcttc
    10321 aggaatgaaa ggcagtttgg ttacgcggct gcctgctgca atgatacagt ttttgaaggc
    10381 aacgattttt ttctcgccgg tagtggctgc ctgttcgtac gcgtcgccgg cagtcagcga
    10441 cacttccaag tggtgcgggt ctaagaattg cccgtcgcct tggataacgt ccactttacg
    10501 gcttttcgcc atacctgcca aaccgcccgt gaggcgggaa actacgccgt ctttgtaggc
    10561 gcgaagcata tcgatgtcga gttccggctc ggggtatttg ataccgttgg cagccaagtg
    10621 gcgcacttcg tcgataacgg cggcattgtg caacaaggct ttggaaggga tacagccgac
    10681 gttcaggcaa acgccgccca aagttttgta acgttcgacg atggcgactt tcaagccttc
    10741 atcggcagcg gcaaatgcag cggagtaacc gccgggaccg ccacccaata cgaccacgtc
    10801 gtactcggca tcggcagaac cgccgaattg cgcggcttgc ggagcaggag cagcagcttt
    10861 aggggcttct tgcgccgggg cggcagcagg tgcttcagct ttgggagcgg ctgccgtgcc
    10921 ttcagcttca acgacgacaa tcaagccgcc ttcggagatt ttgtcgccga ctttgacttt
    10981 cacttctttg accacgcccg caacttcggc aggcacgtcc atcgtcgctt tgtcggtttc
    11041 cagggtaatc agggtatcgt caacggcgat ggtgtctcct acgttcactt caactgcgat
    11101 aatatctaca ttttcgtgtc cgccaatgtc gggcactttc aattcaacta agctcatgtc
    11161 tcatctttct gaatgatgtt caaacttcct tttcagacgg cacaggccgc tatgccgtct
    11221 gaagccgatc gggttacagg gtaatgcggc ggaagtcttt caacagcttc gccaagaata
    11281 cggtaaagcg cataccggcc gcaccgtcga tgacgcggtg gtcgaaagac aggctcaacg
    11341 ggcacatcag gcgtggggca aattctttgc cgttccaaac cggtttgatt tgggatttgc
    11401 acacgcccaa gatggcgact tcgggagcgt tcacaatcgg cgtgaaacct gtgccgccga
    11461 taccgcccaa gctggaaatg gtaaagcacg cgccttgcat ttcttgcggt ttgagcttgc
    11521 cttcgcgggc ttttttagac aattcggtca gctcttggct gatttgtttc aagccttttt
    11581 gatccacgtc tttgatgacg ggaacaacca agccgttcgg cgtatcggct gcgaaaccga
    11641 tgttgaagta gtttttcagc accaggttgt cgccgtccag agaagcgttg aattcgggga
    11701 aggctttcag cgcggaaacg gaggctttga tgatgaacgc tagaggagac agtttcacgc
    11761 cttcgcgttc ccattctttg ttcagttgtt tgcggaattc ttccagctcg gtcatatctg
    11821 cttcttcgtg tacggtaacg tggggaatca ccacccagtt gcgagacagg ttttgaccgg
    11881 agattttctt aatgcgggac aattctttaa cttcgacatt gccgaatttg gagaagtcca
    11941 ctttaggcca cggcagcaag tccagaccgc tgcccaaaga tgcgccggct gcggcaggtt
    12001 ttgccgcgcc accctgcatt acggatttca caaaggcttt gatgtcgtcg cccacgatgc
    12061 ggcctttcaa gccggtacct ttgacttggc ccaaatccac gcccaattcg cgcgccagtt
    12121 tgcgtgcgga aggaccggcg tgtgctttgg cgaaagcggc ctcgtcgatt ttggcagcgg
    12181 caggtgcaga aggtgcaggc gcggcggcgg gtgcggcggc agcagtcgga ggcggagcgg
    12241 cagccggtgc ggcagcttga gcggcttgag caggggctgc tgcggcagag ccgacggttt
    12301 ctacttcgat aatggcagag ccttcggaca ctttgtcgcc gacttttaag aatacggctt
    12361 taacgacacc ggcagcggta caaggtacgt ccattgtcgc tttatcggtt tccaaagtaa
    12421 tcagcgtgtc gtcttcggca acggtgtcgc ccactttgat ttcaacggcg attacatcca
    12481 catcggtatg gccgccgata tcgggaacgg ctacttgaac ggttgcaccg cctgcggcaa
    12541 cgggtgcggc agcagccgag gcggcttcgg cagcggcggc accggtttca acggtcaaaa
    12601 ttacgccgcc ttcggagatt ttgtcgccga ctttgacttt tacttctttc acgacaccgg
    12661 cagcatcggc aggcacatcc atcgtggctt tgtcggtttc cagtgtaatc agggtgtcgt
    12721 caacggcgat ggtgtcgccc gctttaactt ctacggcgat gatgtcgacg ttttcgtgac
    12781 cgccgatatc ggggactttg atttctacga tactcatttg agttccttta atcttgttca
    12841 gttggatgcc gtctgaaagc tgcttcaggc ggcatcggta cggtttgtct gctcaaacca
    12901 ttcgggcatt cggttttcag acggcctgat gtcggaatgg ggcaaacaga ccgtctgaaa
    12961 catcaatcag cgtttccagc taggagctga atcggctttg atgccgtatt tctcaatggc
    13021 ttgttgaacg gtttctttgc tgactttgcc ttgttccgcc aatgcgctca atgctgccac
    13081 ggcaacgttg tagcgatcca cttcaaagaa gcggcgcagg ttggcgcggc tgtcggagcg
    13141 gccgaagccg tcagtaccca agacgtggta gtcgttcggg atgtaggcgc ggatacggtc
    13201 agcatagctg cggatatagt cggtagcggc aataaccgga ccgtcatgac cttgcaattt
    13261 ggaagtaacg aaaggtactt tttcggcttc cagcggatgc aggcggttga agcgttctac
    13321 ctcgacagcg tcgcggtgca gcaggttgaa ggatgggcaa gaccaaatgt ctgcttccac
    13381 gccgaagtcg gctttcagca gctcggcacc ggtaaccact tcttgcaaaa tcgtaccgga
    13441 acccatcagt tggactttct tgtcgccttt gccgccggct ttcagcaggt acataccttt
    13501 caagatgtct tgttccgcac cttcgggcat atccggatgg gtgtagttct cgttcatcag
    13561 ggtgatgtag tagaacacgt cttcattatt ggcatacata cggcgcagac cgtcttgtac
    13621 gatgacggcg acttcgtatt ggaaagtcgg gtcataagat acgcagttcg gaatcaggtc
    13681 ggcctggatg tggctgtggc cgtcttcgtg ttgcaggcct tcgccgttca gcgtcgtacg
    13741 gccggcagta ccgcccagca ggaagccgcg cgcgtgcata tcgcccgccg cccaagccaa
    13801 gtcgccgata cgttggaaac cgaacataga atagtaaatg tagaacggaa tcatcgcgaa
    13861 gtcgctgttg gcgtagctgg ttgcagccgc aatccagtcg gccatcgcgc ccggttcgtt
    13921 aataccttct tgcaagattt gaccgtcaac ggattcttta tagaacatca gttggtcttt
    13981 gtcttgaggg gtatattgct gacctttcgg attccaaata ccgtattggc ggaacatacc
    14041 ttccatgccg aaagtacggc tttcgtcggg aacgataggt acgacgcgtt tgccgatttt
    14101 tttgtctttc agcaaagtgg acaggatgcg gacgaatgcc atcgtggtcg agaactcgcg
    14161 ttcaccgctg gatttgagtt gtgcgtcgaa tgctgacagc tcgggcactt ccaatacttc
    14221 ctgcgtcggt ttgcgttgcg gcaggtagcc gcccaaagca tcgcggcgtg cgtgcaggta
    14281 tttgtattct tccgtatcgg gggcaaaagt caggtaaggc agatcgccgc tttcgatttg
    14341 ttcgtcggta accggaatgt caaagcggtc gcggaattgt ttcagggacg ctttgtccat
    14401 ttttttggct tggtgggcaa cgttctgacc ttcgccggat gcgcccatac cgtaaccttt
    14461 aatggttttc gccaagatga cggtaggttt gccgtcggca tggttcgctg cgcggtcgta
    14521 ggcgttgtac actttttgcg ggtcgtggcc gccgcggttc aatgcccaga gttgctcatc
    14581 ggtcatatcg gcaaccaatg ctttcagttc gggcgtattg aagaagtgtt cgcgcacata
    14641 cgcgccgtct ttggatttgt aagtttggta gtcgccgtcc aaacattctt ccatacgttg
    14701 gcgcaggata ccgtctttgt ctttcgccaa gaggcggtcc cagcggcggc cccaaatgac
    14761 tttgacgaca ttccagccgg cgccggcaaa gttgccttcc aattcttgga tgattttgcc
    14821 gttgccgcgt accggaccgt ccaagcgttg cagattgcag ttgatgacga aaatcaggtt
    14881 gtccaagcct tcgcgtgcag ccagtgcgat tgcaccttga gattcgggtt cgtccatttc
    14941 gccgtcgccg cagaaacacc atactttacg gcctttggtt tttgccaaac cacgcgattc
    15001 caagtatttc aggaaacgcg cctgataaat cgccatgatg ggccccaagc ccatggatac
    15061 ggtcgggaac tgccaaaagt cgggcaagag gtgggggtgc ggataggaag gcagaccgtg
    15121 tccgtccact tcttggcgga agttgttcag ctggtcttcg gtcagacggc cctcgacgaa
    15181 tgcgcgtgca tagatgcccg gggcgacgtg accttggaag aagaccaaat cgccttcttc
    15241 gccttcgcct ttggctttcc aaaagtggtt gaaaccgact tcgtacatgg tggcggcaga
    15301 ttggaaagat gcgatgtgcc cacccagttc caaatctttc ttgccggcgc gcaatacgat
    15361 ggcggcggcg ttccagcgca cgaatgcgcg gatgcggtgt tcgatgtttt ggtcgcccgg
    15421 aatgcctttt tcgttttcaa ccgaaacggt attcaaatac ggtgtggtcg tgccgtgtgg
    15481 catacgtacg cccttgtcgc ggcagtattt gaccaggttt tccaagaggt attgcgcgcg
    15541 ttcgccgcct tcatattcga ggacggagct taacgcgtcc agccactctt gggtttcgat
    15601 agggtcaaca tcgtgtaatt gggtggacat agtatctatc ctttatgttg agtgtttaat
    15661 cggcagggag ggtgtagcca acccctttat tttcgtttgt gaaaaataat aagcaaaaac
    15721 gaaaatttct aaatcaaacc gaaaaaaata atttaagttt aatgttttca aaaaattata
    15781 aaagttttgg aaacaaggga aaacttgaaa aaaatgttcg atctgctcca cacgaaatcc
    15841 tatcagcaat cgggtctgct ggcaaataac catgcctgta aaagcaggtt ttatactgaa
    15901 gttggcgttt ggtaaggtca agtctttttg atttcgaaat attctgtttg attttggcga
    15961 tattttttcg gaattgtggt atatcgaatc attatgtctc tgtcggcaat tttcctattg
    16021 tagaaaatct atacacaagc tttgtggata accgtgttga taagccacgg cttgtgttaa
    16081 aaaagtctta cgcaacaaat gtacgcttat tttgattaaa aaataggcac atttaaatct
    16141 ataaaaatca tgtgcttatg ttcaatatga tgtttctggt catgtttttg ccgtctgtta
    16201 tgggatttct cccgcgcttg gggcaggggg tgaattttgg aggttggtgc aaaacctgct
    16261 tttttcgcgt tacaatctgc acccgattta cttttgaatt tgcctgtttg gaaaaatact
    16321 tttatgaaag ccagccaatt ctttatctct actttaaaag aagcccctgc cgaagccgcg
    16381 cttgccagcc acaagctgat gattcgcgcc ggtctgatta aagccaacgc gtccggcctt
    16441 tatacttgga tgccgatggg gctgcgcgtg ttacgcaaag tcgaaaacgt cgtgcgcgag
    16501 gaaatggcgc gcgcgggcag cgtggagctg ctgatgccgg tggtgcagcc tgccgagctg
    16561 tggcaggaat ccggccgctg ggaattttac ggtaaagaac tgctgcgcct gaaagaccgc
    16621 cacgaccgcg atttctgcat gggcccgacc tgcgaggaag tcatcgccga catcgtgcgc
    16681 aaagaaatca acagctacaa acagctgccg aaaaactttt accacatcca aaccaaattc
    16741 cgcgacgaag tgcgcccgcg tttcggcgtg atgcgcgcgc gcgagttcgt catgaaagac
    16801 gcttattcct tccatgccga ctacgcctcg cttcagacga cctatgatgc catgtatgac
    16861 tcttactgcc gcatcttcac ccgtctgggc ttggcattcc gccccgtcgc cgcagacacc
    16921 ggcagcatcg gcggtacggg ttcgcacgag tttcaagtgt tggcggaaag cggcgaagat
    16981 gttattgcgt acagcgacac ttccgattac gccgccaata tcgagttggc accgaccttg
    17041 ccgcttaaag gcgaacgcgc cgctgctcag gctgtgttga ccaaagtaca tacaccaaac
    17101 gtcaaaacca ttgagtcttt ggttgaattc ctgaatattc cggttgaaca aaccctcaaa
    17161 tccatcgtgg tcgaaggcga aaacgaaggc gaaatcgtct tattactgtt gcgcggcgac
    17221 catgagttca acgacatcaa ggcagaaaaa ctcgcaggcg tgagatcgcc attgacgatg
    17281 gcaagtcctg ccgcgattgt cgaacagttc ggcgcaaacg gcggctcgct gggtccggtc
    17341 ggctttgcag gtaaagttta tgccgatttc gccactgaaa aaggcgcgga ctgggtcatc
    17401 ggtgcgaacg aagacggcta ccactatacc ggcttcaact tcgggcgcga tgccgccgag
    17461 cctgaattcg tcgatttgcg taatgtggtc gaaggcgacg aaagccccga cgggcaaggc
    17521 cgtctgaaac tggcgcgcgg cattgaagtc ggacacgtct tccagttgcg cgacaaatac
    17581 acccaagcca tgaacgtaag cttccttgac aacaacggca aatcgcaaat tatggaaatg
    17641 ggctgctacg gcatcggcat cacccgcgtc gttgccgccg ccatcgagca gaataacgac
    17701 gaaaaaggca tcatctggac caaagcaatg gcgccgtttg aagtcgttat cgtgccgatg
    17761 aactacaaaa aatcagacac cgtacgcgaa gccgccgaca aaatctatgc cgaattgctg
    17821 gcggcaggcg cggatgtgct gctggatgac cgcgacgaac gcgcaggcgt attgctgaac
    17881 gattccgagc tgttgggtat tccgcaccgc atcgtcatcg gcgaccgcgc tttgaaagaa
    17941 ggcaatgtcg aatacgccga acgccgcaac aacgaagcgc aggcagttgc aatcggagaa
    18001 attgttgtgc gtgtaacagc tttattaaat gggtaagctt ggtcaaatgc cgtctgaaaa
    18061 taatttcaga cggcattttt atttggggag aaagtgaagt ggcttcagta tttttagaag
    18121 gagaagcagt cgaggtcggc aatattttct gtatcggcag gaactatgcc gcacatattg
    18181 aagagttaaa aaacgaaata ccttccgaac ctgtggtgtt tatgaagccg tcaggcagca
    18241 ttttgaacag cggcggtaca atcttgctgc ccgagtttag tagggatgtg cagtttgagt
    18301 gcgaactcgt gctgctggtc ggtaaggatt cagacggcac gggcgagggt aaagatattt
    18361 tgggatgtgt cgcaggatat ggtgtagggc tggaccttac ggcacgggat atccaatgcc
    18421 gtctgaagga aaaagggctg ccctggctga aggcaaaagg gttcaggcat tccgcgtgcg
    18481 tgtcggattt tgcggcggca ggcagaatcg gcaatcccga gaatgtgttg ttttcattaa
    18541 aacaaaacgg cgtattgaaa cagcgcgggg ataccggttt gatgatttac ccgattcggg
    18601 aaattttaca caaactggcg gcagattacg gtttggggaa aggggatttg gtgtttaccg
    18661 gaacgccctc cggcgtgggc gcaatcggtg cgggggacaa tttggcattg gagctggacg
    18721 gcttggtgcg cgcctctttc acggtagggt gttaaccgcg ccgcttaacc ctcacgcccg
    18781 ttgaaacatt ccgccggacc gccctcgaaa aaaccgtgca agatggcttg cgccgccact
    18841 tggtcgagca ccgatttgcg ttttttgccg aagacctgtg cttccgaaag caggctttcg
    18901 gcatagacgg acgacagccg ttcgtcaacc caatagacgg ggagattgaa cctgccgttc
    18961 agcctgcgtc cgaacttgcg cgacaggtgc gtcatttcat gtttcgtgcc gtcggtatgc
    19021 acgggcagtc cgacgacaaa ataacgcggc tgccattctt gaaccagctt ggcgattgcc
    19081 gcgaactttt catcgttgct gccgccggta acggtgctca aaggatggga tagccctaat
    19141 tccgcgtctc cttgtgccac gccgatacgc gcttcgccga agtcgaatgc cagtgccgtt
    19201 ccttttggaa ttttatgcat ggccggctcc cgaaaacagg gcgagcgggt cgatgccgag
    19261 tttggcgaat gcggcggcgt aacggtgttc gtaggggatg tcgaacagga tgtgttcgtc
    19321 ggcgggaaca gtcagccacg cattgtcggc aagttcgcgt tcgagctggc cttttcccca
    19381 gcttgaatag cctatgctga tcaaggcttt gtcaaccgca ccttcgcgtg aaatattttc
    19441 aatcacgtct cgggaagaag ttagcgcgat attgtctgaa acgccgatac tgctttgcca
    19501 gttgccgatc ggggtatgca cgacataacc tcgctcgact tgcaccggcc cgcccatcat
    19561 cacgctgtcg tgctgcatcc gcatggggat gtttttgccg gtggcggaaa aaatcatgtc
    19621 catcgtaatc ggagagggtt tgttgatggc gatgccgagt gcgccgtctt catcgtgttt
    19681 gcagatatag acgaccgatt gtgaaaaaaa cgcgtcttcc atatcgggca tggcgacgag
    19741 aaagtggttg gagaggttca tatcagcgtt tgtcgggatt ttctatcggg ttggaaatca
    19801 tgcaggcgcc ggcaagcagg ttgccggaag tatcggcttg caccccatcg gtttgcggta
    19861 tgccgccgcc gaaacaggaa accgcgctta aggaattttt cggcgagcct tggatgattt
    19921 tatcgctgta caccaaatag gcgaaggttt tgcgtttggg gtcgtaataa cggacaatct
    19981 gccggctctt gaacgcgaag ctcgcaccgt gtttgaaaac ttctttcggt ttgcgcacgg
    20041 cggtttcgtc aaaagaaatc gaagatgccg tctgaacgca cgaaaccgat gcgtcggacg
    20101 cgtcctcttc caaattgacc atttccttca agccgccttt ttttgcatac gaaatataac
    20161 aggcaacccc ttgaacgtcg ggatcgtcga atccttccac ttcgatacgg tcgtttttgc
    20221 ccagtatgtt gaaaacggta cttgcccgtc cgattttatc ggtttcgccg ctgccgcagg
    20281 cagtcagcag gacggcggca gacagcagta gcagtctgtt catcaaaatt tccttatcgg
    20341 ttgaatcccc gttttccgtg aaggcggcag ggcttgcttt gtttggaggg gcgcacgttc
    20401 ccaaaatcgg gcaacatacg gtatgcggct taatgtgcta ccctgtgtgt cggaacacaa
    20461 tatccgttgc ggcattgaaa atatgacgca caatataagg ctgtttgagc cgattgcaag
    20521 tatccgcgca aagaaggcgc aagattgggc aaacggcatt ttcagacggc ataactgaca
    20581 gtataatcca aacgtccggc tgctttcgtg cagccttcag tattatccaa ctttttccga
    20641 aggtcgaacc atgcgctaca aaccccttct gcttgccctg atgctcgttt tttccacgcc
    20701 cgccgttgcc gcccacgacg cggcacacaa ccgttccgcc gaagtgaaaa aacagacgaa
    20761 gaacaaaaaa gaacagcccg aagcggcgga aggcaaaaaa gaaaaaggca aaaatggcgc
    20821 agtgaaagat aaaaaaacag gcggcaaaga ggcggcaaaa gagggcaaag agtccaaaaa
    20881 aaccgccaaa aaccgcaaag aagcagagaa ggaggcgaca tccaggcagt ctgcgcgcaa
    20941 aggacgcgaa ggggataaga aatcgaaggc ggaacacaaa aaggcacatg gcaagtccgt
    21001 gtccggatcc aaagaaaaaa acgcaaaaac acagcctgaa aacaaacaag gcaaaaaaga
    21061 ggcaaaagga cagggcaatc cgcgcaaggg cggcaaggcg gaaaaagaca ctgtttctgc
    21121 aaataaaaaa gtccgttccg acaagaacgg caaagcagtg aaacaggaca aaaaatacag
    21181 ggaagagaaa aatgccaaaa ccgattccga cgaattgaaa gccgccgttg ccgctgccac
    21241 caatgatgtc gaaaacaaaa aagccctgct caaacaaagc gaaggaatgc tgcttcatgt
    21301 cagcaattcc ctcaaacagc ttcaggaaga gcgtatccgc caagagcgta tccgtcaggc
    21361 gcgcggcaac cttgcttccg tcaaccgcaa acagcgcgag gcttgggaca agttccaaaa
    21421 actcaatacc gagctgaacc gtttgaaaac ggaagtcgcc gctacgaaag cgcagatttc
    21481 ccgtttcgta tcggggaact ataaaaacag ccagccgaat gcggttgccc tgttcctgaa
    21541 aaacgccgaa ccgggtcaga aaaaccgctt tttgcgttat acgcgttatg taaacgcctc
    21601 caatcgggaa gttgtcaagg atttggaaaa acagcagaag gctttggcgg tacaagagca
    21661 gaaaatcaac aatgagcttg cccgtttgaa gaaaattcag gcaaacgtgc aatctctgct
    21721 gaaaaaacag ggtgtaaccg atgcggcgga acagacggaa agccgcagac agaatgccaa
    21781 aatcgccaaa gatgcccgaa aactgctgga acagaaaggg aacgagcagc agctgaacaa
    21841 gctcttgagc aatttggaga agaaaaaggc cgaacaccgc attcaggatg cggaagcaaa
    21901 aagaaaattg gctgaagcca gactggcggc agccgaaaaa gccagaaaag aagcggcgca
    21961 gcagaaggct gaagcacgac gtgcggaaat gtccaacctg accgccgaag acaggaacat
    22021 ccaagcgcct tcggttatgg gtatcggcag tgccgacggt ttcagccgca tgcaaggacg
    22081 tttgaaaaaa ccggttgacg gtgtgccgac cggacttttc gggcagaacc ggagcggcgg
    22141 cgatgtttgg aaaggcgtgt tctattccac tgcaccggca acggttgaaa gcattgcgcc
    22201 gggaacggta agctatgcgg acgagttgga cggctacggc aaagtggtcg tggtcgatca
    22261 cggcgagaac tacatcagca tctatgccgg tttgagcgaa atttccgtcg gcaagggtta
    22321 tatggtcgcg gcaggaagca aaatcggctc gagcgggtcg ctgccggacg gggaagaggg
    22381 gctttacctg caaatacgtt atcaaggtca ggtattgaac ccttcgagct ggatacgttg
    22441 atcggattgc cgaaacggta ggctttatgc aaatcgtgcc gatatggtgc gctgttccgt
    22501 gaagtttgcc gtttgccgta cctgtatttt ccggtttttg gaaatcaaga gagaaagtta
    22561 aagaagaatg tcgaaacctg tttttaagaa aatcgcactt tatactttgg gtgcaatcag
    22621 cggcgtggcc gtcagtctgg cggtgcaggg ttttgccgcc gagaaggaca ggcgggataa
    22681 cgaagtcctg ccggtgcaat ccatccgcac aatggcggag gtttacggtc aaatcaaggc
    22741 aaactactat caggacaaac ccgatgccga tttgtttgaa ggtgcgatga agggtatggt
    22801 ggccggtttg gatccgcatt ccgaatatat ggataaaaaa ggttatgccg agataaagga
    22861 gtccaccagc ggcgaatttg gcggcttggg gatggaaatc gggcaggaag acggatttgt
    22921 caaagtggtt tcgccgattg aggacacgcc tgcggaacgg gcgggggtga aaagcggcga
    22981 tttcattgtg aaaatcgata atgtttcgac acgcggcatg acggtcagcg aagcggtgaa
    23041 gaaaatgcgg ggcaagccgg gtacgaagat tactttgacg ctgtcgcgca aaaatgccga
    23101 caagccgata gtcgtcaacc tgacccgtgc cattattaaa gtgaaaagcg tccgccatca
    23161 cctgatcgaa cccgattacg gctatatccg cgtgtcgcag ttccaagagc ggacggtcga
    23221 aagcgtcaat accgccgcaa aagagctggt aaaggaaaat aaaggaaaac cgctcaaggg
    23281 gctggtgttg gatttgcgcg acgaccccgg cgggcttttg actggcgcgg tcggcgtgtc
    23341 ggcggcattt ctgccgtctg aagcagtcgt cgtcagcacc aagggacgcg acggcaaaga
    23401 ccgcatggta ctgaaagcca ttcctgaaga ttatgtgtac gggatgggcg gcgattcgtt
    23461 ggcgggcatt cctgccgagt tgaaaaccat accgatgacg gttctgatta attcgggttc
    23521 ggcttccgcg tcggagattg tcgcaggtgc attgcaggat cataaacgcg cggtcatcgt
    23581 cggtacgcag agcttcggca aaggttcggt tcagactttg attcctttgt ccaacggcag
    23641 cgcggtcaag ctgacaacgg cactgtatta tacgccgaac gaccgttcta ttcaggcgca
    23701 ggggattgtt cccgatgtcg aagtaaaaga taaggaacgc atttttgaaa gccgcgaggc
    23761 ggatttggtc ggacacatcg gcaatccttt gggcggcgag gatgtgaaca gtgaaaccct
    23821 tgccgtgccg cttgaaaaag atgcggataa gcccgctgta aaagaaaaag gtaaaaagaa
    23881 aaaggacgag gatttgtctt caaggcggat tcccaaccct gccaaagacg accagttgcg
    23941 gaaagctttg gatttagtca agtcgcccga gcagtggcag aagtctttgg ggctggcggc
    24001 gaaaaagccg gtttcaaata aagataagaa agataaaaaa gataagaagt aggttttgac
    24061 gatgccgtct gaaaggattt cagacggcat attatgtttg aaaggacagg ttatgagcga
    24121 ttttttaccg gaatttgaac cgtgcggcat ttatagtgga ttaacaaaaa tcaggacaag
    24181 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt
    24241 ttgttaatcc actatacttg ggtagcgatt gggtttcaga cggcatacat tcaggatttc
    24301 accgccttat gccgtctgaa agacgtttcc gacccatccc tactacacac tttgtccgca
    24361 aacgcatcac aggcaagatt atggaagtta tccaataccc aaattccccc ttcaaactcc
    24421 accaaccctt cccgcccgca ggcgaccagc ccaccgccat tgcaggcctg ctcgaagggc
    24481 tttcagacgg ccttgcctat caaaccctgc tcggcgtaac cggttcgggc aaaacctaca
    24541 ccatggcgaa cgtcatcgcc caaagcggcc gccccgccat catcatggcg cacaacaaaa
    24601 cccttgccgc ccagctttac gccgaaatgc gcgaattttt ccccgaaaac gcagtggaat
    24661 atttcgtctc ctactacgac tattaccagc ccgaagccta tgtgcccagc cgcgacttgt
    24721 tcatcgaaaa agacagcgcg atcaatgaac acatcgaaca aatgcgcctt tccgccacca
    24781 aaaacctgat gacgcgcgac gacgtgatta tcgtcgccac cgtgtccgcc atttacggta
    24841 tcggcgaccc gaccgagtat caacaaatgg tgttgtccgt caaagaaggc gataccatag
    24901 cccagcgcga catcatcgcc acgctcgttt ccatgcagta cgaacgcggc gatttggact
    24961 tcaaacgcgg cagcttccgc gtgcgcggcg acgtgattga cgtgtacccc gccgaaagct
    25021 ccgaaaacgc cttgcgtatc agcctgttcg acgacgaaat cgaccgcctc gatatgttcg
    25081 acccgctttc aggcagcctt attcaacgcg tcggccgcta caccgtcttc ccgtccagcc
    25141 actacgttac cccgcgcgac accgtattgc gcgcctgcga gtccatcaaa gaagaattgc
    25201 gcgaacgcat cgaatttttc gcccgcgaac aacgccccgt cgagcaacaa cgcatcgaac
    25261 aacgcacccg cttcgacctc gaaatgctct acgaaatggg cttctgcaaa ggcatcgaaa
    25321 attattcccg ccacttctcc ggcaaaaaag aaggcgaacc gccgcccacg ctgatggact
    25381 atctgcccga caacgccatc atgttcatcg acgaaagcca cgttaccgtt acccaaatcg
    25441 gcggcatgta caaaggcgac gcatcgcgca aacaaaacct cgtcgactac ggcttccgcc
    25501 tgccttccgc ccgcgacaac cgcccgctca aattccacga atttgaaaaa gtcatgccgc
    25561 aaaccgtctt cgtttccgct actcccgcca aatacgaaga agaacacgcc ggacaagtgg
    25621 tcgaacaagt cgtccgccct acagggctgg tcgaccccca aatcatcatc cgccccgtcg
    25681 ccacccaagt cgatgactta atgagcgaaa tcaacgaccg catccaaaaa ggcgaacgcg
    25741 ttctcgttac caccctcacc aaacgcatgg cggagcaact caccgactat tacagcgaac
    25801 tcggtatcaa agtgcgctac ctgcacagcg acatcgacac cgtagagcgc gttgaaatta
    25861 ttagagattt gcggcttgga ctgtttgacg tacttgtcgg catcaacctc ttgcgcgaag
    25921 gcttggacat ccccgaagtc tcccttgtcg ccatcctcga cgccgacaaa gaaggcttcc
    25981 tgcgctcgca ccgcagcctg attcaaacca taggccgcgc cgcgcgcaac gtgaatggcg
    26041 ttgcaatttt gtatgccgac aaaatcaccg actccatgaa agctgccatc gacgaaaccg
    26101 aacgccgccg cgaaaaacaa attaaattca acgaagaaca cggcatcgtg ccgcagcaga
    26161 ttaaaaaaca ggtcaaagac atcatcgacg gcgtgtacca cgaagaagac agcggcaaag
    26221 gccgtagaca aggcaaaaac aaagttaaag tcggggagat tcataacgaa gaagacgcga
    26281 ttaaagaaat cgccaaactg gaaaaagcca tgcagcaggc ggctcgggat ttgcagtttg
    26341 aggaagcggc ggttatgcgt gatagaatac ggaatattaa ggaaagcctg ttgtttgggg
    26401 cagagtaatg aaatagttta atattctcag cttaatgtat aaaggataat tttatgaaat
    26461 tgtcaaaaac aagaatagat aaatctggct tagcattggc taaacaacaa tataaagatg
    26521 aaattgattt tattgaattg gaagaatgtt ttgatttgta taggcaatca catctttcac
    26581 cattatctaa aacaactatt gatattcaaa atattcttag ggaatatatt ggtgagtatt
    26641 atatagctca gcgactaaaa agaaaaccac agataatcag aaagttaaat aggcttagcg
    26701 ttagactaac tcagttgcaa gacattggtg gttgtagaat tattgttcca acaaatagag
    26761 atgttgataa gatatataag tttttatcaa gtaagaagga taatcttgat tttttaataa
    26821 aaaaagtaac cgattaccgt gaaaaaggta gagatgattc agggtataga gctttacata
    26881 taattataga aagagaaaat cttaactttg aattacaaat tagaagtagg atacaacatt
    26941 attgggcaga aagtatagag aaaacctctg ttgtttatgg atattactta aaagagcaag
    27001 aaggggatag agatgtaatt aactatttta aagaattatc agatgttttt tatgaaattg
    27061 aatcagggag aaaaccaact actatacaaa agttaaatct tgaagattta agaaaaagat
    27121 gtgaagttat tattcagaaa tcagatactc ataaaatatt aaatagctat gttaatgaag
    27181 gtataattag tgcgttgata gaaaaggaaa agagaaatac taattcattg aataactgga
    27241 tactaatttt tgattggaat gcaggggttt tcgttagttg ggatgttgta gctcaagatc
    27301 cgaagcaagc cgttgaggta tatgttaatt atgagaatca gtatccatct ttagaggggt
    27361 tcgaggttgt tttgatagga tcatccgata tcgcttctgt acaaaaaaca catagtcatt
    27421 attttggcat agcctcatat gaaaatattc tagaaacact tgatgaatca attttttgct
    27481 ttgctaaaaa agatgatatt gatattgggg caagaagaat attggcagca atggtaaggc
    27541 ataaattttg gggaacaaaa acagtaacat cagaaacttt aaaaaatcat ttctgccaaa
    27601 atctttttgg atttgattat tctttaaggg atttaataag aaaagaatta ataaaatatg
    27661 gaagagataa agatttatca ttgaatatag caaagaaaat ggaaatagaa tcgtatttat
    27721 aagcaagcgc agcttgtatc cccatcaacc gtgtgcgtgg ctacgccaca caccttacct
    27781 gcgtatggca caaaaacttt aaaaccgagg tgtagggtgt gtgcggtacg cacgcacgcg
    27841 ttctttgtag tgcaaaccac tgcttcaaac tttcagacga cctcaaacgc cgtctgagaa
    27901 tctatggcat aaaggccgtc tgaaaaaatg gtttcagtac aactgccact ggttgtaact
    27961 tgattaaaac caccctttca gacagcctgc cgttttcagc aagcgtaggt cggatacttg
    28021 tatccgacaa aacctttaac attcccatca ttgcaatcca ttgcagcaat gcccaaaatg
    28081 tcgaattcaa gaatccgacc tacaaaatca ttccgagcat aatactatga aataccgtcg
    28141 ttttaccgca atggcggcac ttactttttt acggttgtaa ccaataaacg gcagaagatt
    28201 ttgaccgatg atgcggtgcg tttggcttta cggcaggcgg taatggcggt gcgcgaacgg
    28261 tatccgtttg aaattttggc atgggtgttg atgcccgacc atctgcatac catatggcgg
    28321 ctgccggaca atgattctgc ttattcggaa cgctggcggc agatcaagcg gcacagcaaa
    28381 tatttaatcg gcggcaatct caggctttgg caaaaaccct tttgggaaca tactatccgc
    28441 gatgaggccg attttgcctg ccattttgat tatctgcatt tcaatccggt caaacatggc
    28501 tatgtaggac aaatttccga ttggcggttt tctacgtttc accgttatgt caaacagggt
    28561 atttatccgc ataattgggg tggcggcaat gcggactttt ctattggata cgattgaagt
    28621 aatgtcggat tcgagaatcc gacctacgga aaactgaaag agcatcggct gcaggacggc
    28681 attatgcgca aagcctgccg caaccgtccg ctgtcggagg cgcaaaccaa acgcaaccgg
    28741 tatttgtcga agacccgtta tgtggtcgaa cagagcttcg gtacgctgca ccgtaaattc
    28801 cgctacgctc gggcagccta tttcggactg attaagtgag
//
LOCUS       DAXIHS010000018        28307 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXIHS010000018 DAXIHS010000000
VERSION     DAXIHS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.018"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            356..1660
                     /locus_tag="ACFOF2_000669"
     CDS             356..1660
                     /locus_tag="ACFOF2_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC7107026.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
     gene            complement(1905..3200)
                     /locus_tag="ACFOF2_000670"
     CDS             complement(1905..3200)
                     /locus_tag="ACFOF2_000670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC7107027.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(3326..4213)
                     /locus_tag="ACFOF2_000671"
     CDS             complement(3326..4213)
                     /locus_tag="ACFOF2_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC7107028.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(4213..5178)
                     /locus_tag="ACFOF2_000672"
     CDS             complement(4213..5178)
                     /locus_tag="ACFOF2_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC7107029.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(5194..6318)
                     /gene="potA"
                     /locus_tag="ACFOF2_000673"
     CDS             complement(5194..6318)
                     /gene="potA"
                     /locus_tag="ACFOF2_000673"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC7107030.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLSNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(6514..6621)
                     /locus_tag="ACFOF2_000674"
                     /pseudo
     CDS             complement(6514..6621)
                     /locus_tag="ACFOF2_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6700..6969)
                     /gene="rpsO"
                     /locus_tag="ACFOF2_000675"
     CDS             complement(6700..6969)
                     /gene="rpsO"
                     /locus_tag="ACFOF2_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC7107031.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(7192..8127)
                     /gene="secF"
                     /locus_tag="ACFOF2_000676"
     CDS             complement(7192..8127)
                     /gene="secF"
                     /locus_tag="ACFOF2_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC7107032.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGTALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(8131..9984)
                     /gene="secD"
                     /locus_tag="ACFOF2_000677"
     CDS             complement(8131..9984)
                     /gene="secD"
                     /locus_tag="ACFOF2_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC7107033.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(10196..10462)
                     /gene="yajC"
                     /locus_tag="ACFOF2_000678"
     CDS             complement(10196..10462)
                     /gene="yajC"
                     /locus_tag="ACFOF2_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC7107034.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            10590..11699
                     /locus_tag="ACFOF2_000679"
     CDS             10590..11699
                     /locus_tag="ACFOF2_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC7107035.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRYDI"
     gene            12062..13126
                     /locus_tag="ACFOF2_000680"
     CDS             12062..13126
                     /locus_tag="ACFOF2_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC7107036.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            13267..13590
                     /locus_tag="ACFOF2_000681"
     CDS             13267..13590
                     /locus_tag="ACFOF2_000681"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC7107037.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            13662..13985
                     /locus_tag="ACFOF2_000682"
     CDS             13662..13985
                     /locus_tag="ACFOF2_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC7107038.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            14032..14235
                     /gene="tatA"
                     /locus_tag="ACFOF2_000683"
     CDS             14032..14235
                     /gene="tatA"
                     /locus_tag="ACFOF2_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC7107039.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            14239..14925
                     /gene="tatB"
                     /locus_tag="ACFOF2_000684"
     CDS             14239..14925
                     /gene="tatB"
                     /locus_tag="ACFOF2_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC7107040.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANPASDGISDVMPSERSYTSAETLGDDRQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            14939..15709
                     /gene="tatC"
                     /locus_tag="ACFOF2_000685"
     CDS             14939..15709
                     /gene="tatC"
                     /locus_tag="ACFOF2_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC7107041.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            15762..16367
                     /locus_tag="ACFOF2_000686"
     CDS             15762..16367
                     /locus_tag="ACFOF2_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC7107042.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            16383..16736
                     /locus_tag="ACFOF2_000687"
     CDS             16383..16736
                     /locus_tag="ACFOF2_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC7107043.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            16726..18540
                     /locus_tag="ACFOF2_000688"
     CDS             16726..18540
                     /locus_tag="ACFOF2_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC7107044.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            18730..19407
                     /gene="misR"
                     /locus_tag="ACFOF2_000689"
     CDS             18730..19407
                     /gene="misR"
                     /locus_tag="ACFOF2_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC7107045.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            19420..20826
                     /gene="misS"
                     /locus_tag="ACFOF2_000690"
     CDS             19420..20826
                     /gene="misS"
                     /locus_tag="ACFOF2_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC7107046.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            20944..23370
                     /locus_tag="ACFOF2_000691"
     CDS             20944..23370
                     /locus_tag="ACFOF2_000691"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC7107047.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHSLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKNLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            23415..23660
                     /gene="rpsP"
                     /locus_tag="ACFOF2_000692"
     CDS             23415..23660
                     /gene="rpsP"
                     /locus_tag="ACFOF2_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC7107048.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            23676..24185
                     /gene="rimM"
                     /locus_tag="ACFOF2_000693"
     CDS             23676..24185
                     /gene="rimM"
                     /locus_tag="ACFOF2_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC7107049.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            24185..24934
                     /gene="trmD"
                     /locus_tag="ACFOF2_000694"
     CDS             24185..24934
                     /gene="trmD"
                     /locus_tag="ACFOF2_000694"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC7107050.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            24950..25315
                     /gene="rplS"
                     /locus_tag="ACFOF2_000695"
     CDS             24950..25315
                     /gene="rplS"
                     /locus_tag="ACFOF2_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC7107051.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            25334..25639
                     /locus_tag="ACFOF2_000696"
     CDS             25334..25639
                     /locus_tag="ACFOF2_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107052.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPRSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHIIITALPLSLSKPQIIMTPSLLLSGLTFRLVLALIAVSLLWGVYFWAVSA"
     gene            25636..26361
                     /locus_tag="ACFOF2_000697"
     CDS             25636..26361
                     /locus_tag="ACFOF2_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC7107053.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            26396..27271
                     /locus_tag="ACFOF2_000698"
     CDS             26396..27271
                     /locus_tag="ACFOF2_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC7107054.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            27348..28262
                     /locus_tag="ACFOF2_000699"
     CDS             27348..28262
                     /locus_tag="ACFOF2_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC7107055.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
BASE COUNT         7230 a         8402 c         6633 g         6042 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggaatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtct ggagcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggagaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaacatata
     1741 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa
     1801 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
     1861 acgctgtact ggtttttgtt aatccactat actgcctgcg acgcttaacg gctgtctttc
     1921 cactgataat atttcgagcc gaggaaagaa ccgagtttgc gcaaaaacgg ttgcaggata
     1981 atattcggct ggcgcaaccg ctcaaacggc ttcagacggc attcgtcccc taaaattgct
     2041 tcggcaaccg ccagacctgc aatgcctgtt atcgccatcc cgtgtccgga ataaccttgt
     2101 gcataaaaaa catttgcggc caaacgtccg aaatgcggga caaggttggc ggtaatgtcg
     2161 cactccccgc cccacgaata ttcgattttg acatcgtcaa gctgcggaaa aactttaagc
     2221 atatcttggc ggacaagctc ggtcatacgc gcaggattgt cgataaactc gttatcctta
     2281 ccgccgaaaa gcagtctgcc gtccgcgctg aggcggtaat aatccaaaat atggcggttg
     2341 tcgcatactg ccatattgtt acggataagc ccttttgcgc gcgcccccaa gggttcggtc
     2401 gcaataataa aggtgctaac ggcaatcgcc ttgcgttcca aaggctggaa tatcgggttc
     2461 aaacctgcat aggtattgac ggcatacacc agatttttgc attcgacgct gccttcgggc
     2521 gtgtaaacca gccaaccgtt ttgatgcggt tcgatgcgcg tcatcgggga ctgctcgaaa
     2581 atctgcgcgc cggcttcggc agcggcaccg gcaacaccca acgtgtaatt gagcggatgc
     2641 aggtgtccgg ataaggggtc gaattgtgcc ccttggtaca tatcgctgtc aagctgctgt
     2701 ttcaactcgg ctttatccca aagttgataa tgactcgcac cgtaatgccg ttgggcgtgt
     2761 tcatgccact gctgcaactc ttcccaatgc tgcggacgga cggcaaccgt ggcataaccg
     2821 cgctgccaat cgcaatcaat ggcatgtttg cggacgcgtt cgtccaccag ttcgaccgcc
     2881 tgcaaagact gttgccaaaa ccattgcgcc tgctccaagc cgacctgttt ttcaatttcc
     2941 cccataccgc aggcgtaatc gctgataacc tgcccgccac tccgtcccga cgcgccgaaa
     3001 ccgatacgcg cggcttccaa cacaaccgtt tcatgtccct gctccgccaa gggcaatgca
     3061 gtgcacaaac cactcaatcc gccgccgatg atacaggtat cggttttcag acggcattga
     3121 agtttcggat aaacagtatg aggattaacc gaactgaaat aataagaagg cagatattct
     3181 tgaaaatcag ggcgaatcat tgtgtttgct ttatcaggtg tattttcgga cggaatgata
     3241 caggctgtcg ggccatatcg tccaaacaga aaatcggttg aagaaaacag gctgacccag
     3301 tcatgcggtc agcctgcctt attaattaat ttgctttctc ggcagccaat ttttcttggc
     3361 ggtaggcttc tgccgcttct cggtcacgct tggttgcctg cctcatcatc caataattga
     3421 cgatgatgac caatgttccg atgatgccga ttaggatggt cgccaagaca ttcatctgcg
     3481 gatcgagacc caacttgatt ttggagaaaa tcacctgcgg caatgtggat gaaccggggc
     3541 cggagaggaa tgaggtaatc accaaatcat ccaaagacag ggtaatgccg agcagaaagc
     3601 ctgaagcgat ggcaggggca atcaaaggca aagtgatgac aaaaaagatt ttcagcggac
     3661 gcgcgcccaa atccattgcg gcttcttcga gcgactggtc aagctcaacc agacgcgaac
     3721 ggataacaac ggtaatgtac gccatacaca gcgtcgtatg tccgaggaag atggtgaaaa
     3781 agccacgatc gaagtagaga tgttgtaacc attcgctgcc ctgcaaaaat atctgtacct
     3841 gaataatcag cagcagcata gacagaccgg taatcacgtc gggcatcacc ataggtgcgg
     3901 aaatcatacc ggcgaacaag gtactgccgc gaaaacgttt aatccgcgcc atcgcatagc
     3961 ctgccagcgt gcccaaaacg acggcagcaa gcgaagacac aacggcaatc cgcagcgaca
     4021 gccaagcggc ttccaagatg gtgtcgtttt ccagcaatgc gccgtaccac ttggtcgaaa
     4081 agccgcccca aacggttacc agcttggatt cgttaaacga atagatgacc aaaacaacca
     4141 gcgggatata cagaaacgcc agcgacagtg ccaacatcag tttcaagaac caagataatt
     4201 tggatttctg cattatttgg ctccttcttc caattcgcgg ttttcataat gctgaaacag
     4261 ggcaatcggc acgaccagca gcgcgaccat cacgacggcg acggcggaag ccagcggcca
     4321 gttgttttga tcgaagaacg cctgccacaa gactttacca atcatcaggt tttccgaacc
     4381 gccgaccagc tcgggaatga cgaactcgcc gacagcaggg acgaaaacca gcatggagcc
     4441 tgcaataatg ccggttttcg acaaaggcag ggtaatcgtc aagaacgatt tgaccggccc
     4501 cgcgcccaaa tcggaagccg cttcaagcag gcggttgtcg agtttcacca gttgcgtgta
     4561 tagcggcaga atcataaacg gcagataggc gtaaaccatc accaaattga gcgaaaaggc
     4621 attgtagaac aaatccaaag gctcgctgat aatacccatt ttaatcaaca ggttgtttac
     4681 aatgccgtta tgcccgagca gacccatcca cgcatagacg cgcaacagga acgatgtcca
     4741 aaagggcagc ataatggcaa gcagcaaacc attgcggaca gaaggattgg cacgagaaat
     4801 cgcataggcg gtcggataac cgaccaacag acaaattacc gtcgtagtca gcgcagtctt
     4861 aattgaagac caataagtca tcagatagat attgctgttt tcaccgtcgc cgaacggatt
     4921 gagcgtactc caaaaatttt ggaagatgtc tgcataattt tggtagctga cagcaatatt
     4981 cagacgaccc aaatcctcat ctatcgtcgt taaaggagta aacggcggga tggcgatttc
     5041 ttgttcggca aagctgattt tcagcacgat ggcgaacgga atcagaaaca gcaccaaaag
     5101 ccaaatatac ggtacggcaa tcaccgcacg ctgccccgga cggcggaaca gtttgttttt
     5161 cagtttatta aggttcattg cattcccctt aaatcaacgg aacagtggcg taggctggtt
     5221 ttccggccag ctgatataga cggtttcgtc ccaagttggc ggtgtaatgt tgcgcacata
     5281 ccagtaaggg gcggggactt ggctcttgac gacgcgcccg ttggagagtt tgatatggta
     5341 tatggcgaag ctgcccaaat aggcgatttc ttttaccgtg cctttcgccc agttgtagtc
     5401 gcccaaatat tcgggttttt ctttatataa atcaatatcc tctggtcgaa tactaaccca
     5461 aaggtcctgc tcgctcggac cacccaaacc gtggtcgatg cggacgtggt tttccaaacc
     5521 ttcgcattcg ataacggcat agtcggcatg atcttcaatc accacaccgt caaagatgtt
     5581 ggtttcgccg ataaactcgg cagtgaagcg gctgttggga tagtcgtaca cgtcgctggg
     5641 tgtgccgact tgctgcaact gaccgtcgga cataatggcg atgcgggtcg ccatcgtcat
     5701 cgcctcttct tggtcgtgtg taaccataat acaggttacg ccgacttgtt ccagcgtatt
     5761 gaccaactca agctgggttt gctggcgcag ttttttgtcc aacgcgccga ggggctcatc
     5821 cagcagtaga attttcggac gttttgccag actgcgtgcc aaagcaatgc gctgctgctg
     5881 accgccggac aattggtgcg gtttgcgttt agcaaatttg gtcatctgaa ccaggcggag
     5941 catttcttcg acgcgcgcgg cgatttcgcc tttaggcatt ttgtcctgtt tcagaccgaa
     6001 ggcaatgttt tgttctacgg tcatatgcgg aaaaagcgcg taactttgga acatcatatt
     6061 gatggggcga tcatagggtg caagtttggt aatatcctga ccatcaagga taatttttcc
     6121 ctgattggga ctttccatac ccgccagcat acgcagcagc gtggattttc cgctgccgga
     6181 actgcccaaa agagcgaaaa tttcgtgttg ataaatgtcc aagtcgatgt tatcgacagc
     6241 gtaattgtca ccaaactttt tcaccaaacc ttggatttgc agataaggct tggttgaaga
     6301 cgcagtggtt gcggtcataa tggcaatact ccaataaaaa gacgagtacc ggcaaaacgg
     6361 attttcgaat gggtgataaa aagctgtttg attgctggcg ggagtcgcaa cgtctgatgc
     6421 cgtctgaaac tcttgtgaag cgcacgggca gcatgaaatg gaacaagatt ccaaagaact
     6481 ttatattata ttagtttatg cggttttcgg gcaatatagt ggattaaatt taaaccagta
     6541 cagcgttgcc ttgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     6601 atttaaattt aatccactat aaatatggat ttgagcttgt cggaaagcaa cagaaaagaa
     6661 aacaccgccc atttttctgg gcggtgtcgg aaagcgtaat tatttacgca gacccaagcg
     6721 ggtaatcaac gcgcgatacg tatcgggctg ggtacggcgc aagtaggcca gcaggcggcg
     6781 gcgttggctg accattttca acaggccgcg acggctgtgg tggtcttttg ggttggcttt
     6841 gaagtggggg gtcaggtcgt tgatgcggaa agtcaacaga gcgacttgta cttcggaaga
     6901 gccggtgtcg ccttctttgc gttggaaatc tttaacgatt tgtgcttttt gttctacggt
     6961 cagtgccata atgaaaactc caaaaatata agaatcccca tagggattca gacaagtttg
     7021 ccaagcctga agacaacggc aaactcccta tgcccaagat aggacaacgt ggcattatga
     7081 cacaattccc gcatttctgc acaatatttt aagaccgtgg aagtaaccgt taaaaatgcc
     7141 gtctgaagca ttgtctgctt gagacggcaa tgttcagacg gcatatgcgc ttcaaaccac
     7201 gatttcttct ttcttcttcg gttctttacc gatattgtcg cggctcaaac cgaacattag
     7261 cagaagcggg ctggcaacca atacggaaga ataaatgccg aacacgatgc cgatggtcaa
     7321 cgccatagaa aagccgtgca aggccgtacc gccgaacacc agcatggata cgaccatcgc
     7381 ctcggtcgaa ccgtgggtaa tgatggtgcg gctcatcgtt gcggtaatcg cgttgtcgat
     7441 gacttccggc acggcatgtc cgcgcatcgc cggcttgcgg aagttttcac ggatacggtc
     7501 gaagacgacg acggattcgt tcacagaata gcccaatacg gcaaggatac ccgccaagac
     7561 ggtcagcgaa aattcccatt ggaagaaggc aaagcagccg agaataatca cgatgtcgtg
     7621 catattggcg ataatggcag atacggcaaa acgccattcg aaacgcatcg acaggtaaat
     7681 aatgatgccg ataacgacaa aacctaaagc catcaatcca ttacttacca attcctcacc
     7741 aacttgcggg ccgataaatt cgacttggcg caaagtaacg tcgggactgt cttttttcag
     7801 caaatccata acctgattgg acaactgtgc ggaagtaaca ccttctttgt tcggcaggcg
     7861 gatcatgatg tgtttgttcg tacccaatgc ctgaacctgt acatcaccta ttttcagcgt
     7921 atcgaggcgt tcgcgcatct tattgacatc cgcaccctgc tgatattgga cttccattac
     7981 cgtgccgccg gtaaattcga cagagaaatt cagacctctg gtaaccaaaa agaatacggc
     8041 ggcaataaag gtaaccaacg aaataaaggt cgtcagtttg ccgtagctca taaacggaat
     8101 atcgcgtttg attttaaaga gttccatagc ttactccttg cctcctgcca tttcggcttt
     8161 cggcttccac accgaaccga tggaaatatt ctgcaatttg cgtctgcgtc cataccacag
     8221 attgaccaac gcacggaata cgacgacgga cgaatacatc gaagtcagaa tacccaaaca
     8281 gtgtacgacc gcaaaaccgc gtaccgggcc ggaaccgaat accaaaagcg cgataccggc
     8341 aatcagcgaa gtcaggttgg aatcgacaat ggtcgcccat gcgtgttgga aaccgagatt
     8401 gattgcctgc tgcggcggca cgccggcacg caattcttcg cggatacgtt cgttaatcaa
     8461 gacgttggag tcgattgcca tacccaaagt caacgccagc gcggccatac ccggtaacgt
     8521 caacgttgcc tgcatggcag acaaaatacc gattaggaac agtatgttgg cactcaatgc
     8581 aatggtagaa aagaaaccca tcagacgata gtaaaccacc atgaatgcag caacgatggc
     8641 aaaaccccat aaagtcgaat ggaagccttt ttcgatgttc tccttaccca aagacggacc
     8701 gatggtacgt tcttcgacaa tctgcatcgg cgcggcaaga gaaccggcac gcaacagcaa
     8761 agacgtatca ttggcttcgg ctgtcgtcat gcttccggaa atttccacgc gtccgccggt
     8821 aatggcagta cggataaccg gcgcggttac aacctcggat tttccttggt cgatcaaaac
     8881 catcgccatg cgtttgccga catttgcggc agtcagttcg ccaaaaatgc tgccacccgc
     8941 gctgtccaag ctcagactga cagcaggtgc gcccatttgg tcgaaactcg gttgcgcatc
     9001 gttgatgttg tcgcccgtca gctcgacctg tttgctgatt agcagagttt cgggacgatc
     9061 tccgccgctt gaaagcagct cataaccgct cggcacgttg ccttccaatg cctcgcgcaa
     9121 cttggcagga tcgtcctcca ccatacgcaa ttccaaagtc gcggtacggc cgatgatgtc
     9181 ttttgccttg gcagtatcct gaacgcccgg aagctgcacg acgatacggt cggcaccgga
     9241 ctgctggatg acgggttcgg ccacgcccaa ctcgttcaca cggttgtgca gggtagtgat
     9301 gttctgtttg accgcatcgg aacgcacttt attgaccgcc tcttccgaaa gcgtcaagac
     9361 gatattgctg ccgtctgaat tcagcgttgc ttcaggaaac agcttgcgca actgcggcag
     9421 tgccttttgc acatcacctg catcctgcaa agggacggtc aggctgtttc cagcctgacg
     9481 caccgtgccg ctgcggattt tttcgcggcg cagttcgcgg cggatgtcgc ccgaataacg
     9541 ttcaaacgtt ttctgcatcg ccgctttcat atcgacctgc atggtgaaat gcacgccgcc
     9601 gcgcaggtcc aaacccaaaa acatcggatt ggctttgatt ttcgccatcc attcggggct
     9661 gtccgccaac aggttgagcg cggtaatata cccttcgccc aaagtgtttt cgatgacgtc
     9721 gcgcgcttta agctgcgttt ctgtgtcttt gaaacgcact ttcagtgaat tgtccacaac
     9781 aaacatcccg tcggtctgaa tacctgcgtt tttcagcgcg gcatccactt tggattgagt
     9841 ctgttcgttg atgatgatgg cttgtcggtt ggtcgatacc tgcacggcgg gtgtttcgcc
     9901 gaataggttg ggcagcgaat acactgcggc aaccgcaatc gtgaacacaa tcagcagata
     9961 tttccataaa ggataacggt tcatcattgt tccttaatgg ttggaacccc accctttcgg
    10021 tggtgtcgga atcgggctat ttcagaagag gcaaaaaccc ttcccagcca ggcaagaccg
    10081 gaaggcggca tcttgaatat gccgccctgc gtgtcggaac atggtcaagc cttcggttgg
    10141 aattcaaaac aaagtgccgc attcgggctt tccagatgcg gcttgtcggc acaaatcaat
    10201 cgacttttgc ggcaatcgca ttgcgttcta cttcgacctc gatttttgta ccctgtccga
    10261 tatccacggt aaaaaactgt tcgccgactc tggttacctt acccttgaaa cctgccgcca
    10321 agaccacttt gtcgccgact ttcaaggcgg caagcattgc ctgatgcgct ttgaatttct
    10381 tttgctgcgg acgcatgatc aggaagtaga acaccaccat aatcaacact aaaggagcaa
    10441 attgtgcaac agcttgattc ataatttatc cgttctttct aatatggttg aaaatcgaga
    10501 ggggtatata ataacataag accgtaaaca atatatcggg tttgccgtcc gtaccgaccg
    10561 tataccgcag cctgcccgtc cacaaaccca tgtccttgac ccgcctaatc ttgaaattct
    10621 atgcactgtt gcgccttttt ttgggcaaaa acgcccgcac cgcatggatt tcgcatcccg
    10681 cctgtgccgg gcacgaaccc ggcgcaaacc atcccgattc gcccgaccgc atcctctgca
    10741 tcgagcaggc attgcgccgc gccggtattt ggcagcacct ccaaaccata gaggcggaag
    10801 aaatcagcga tacgcgcctc gcacttgtcc actcgagcaa atatctgaac cgtttggaat
    10861 cttgcctgcc ccaaaaaggc aagatttccc gcctggataa cgacactgca atcagcacag
    10921 gctcgctgtc tgccgcacgc tttgccgccg gttcggcagt tcaggcagtc gacatggtca
    10981 tgaaccgtaa agcatggcat gccttttgcg ccgcccgccc gcccggacac catgcgggca
    11041 gcggcaaagc cggcggattc tgcctgctga acaacgttgc cgccggcgtc atgcatgccg
    11101 ttgccgaata ccggctgaac cgcatcgccg tcatcgattt cgatgtacac tacggcgacg
    11161 gtacggcaga aatattcaaa gacgatccgc gcatcctgtt tttcaacctg tttgaaaccg
    11221 accttttccc cttccccgaa aacaacgata tgcccgacgg cggcaatatg gtgcacctgc
    11281 ccttgccgcc gggaacgggc agccgcgcat tccgcgaagc cgtccgcaga cagtggctgc
    11341 cccggcttgc cgcattcaaa cccgaactgg tgctgctgtc ggcaggattc gacgcacacc
    11401 gtctggacga atcgggcagg cttaacctgc acgaggcgga ttttgcctgg ctgacacaca
    11461 aaatcattca gacggcatcg ggctgccctg gcaaaatcgt atccgtactc gaaggcggct
    11521 acacccttga acctctggca caatctgctg ccgagcatat ccgcgtgctg gcagggctgg
    11581 gcaaatccga tgccgcaacc gcctatcaga aaacactgga cccaacaaaa aaacggtttg
    11641 ccaaaccgaa aaccggacag gtgcgccaac ctacccaatc cgaccgttac gacatatagt
    11701 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacggaacc
    11761 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac
    11821 accgtactgg tttttgttaa tccactataa atgccatctg aagccgattc ggttttcaga
    11881 cggcatcatt atttgacctg acgcaaacag aaagccttcc gcgtgcttaa acacatctct
    11941 tgtagtaaaa tatctaaaca gattcaactg gaaacggtca ggcttcttca cgccattccc
    12001 atttcaaccg ccgcctgcca gattcagacg gtattcctac ttgaaaacaa ggagcgttac
    12061 aatgaaagca gcacgttttt acgacaaagg cgacatccgc atcgaagaca tccccgaacc
    12121 gaccgtcgcc cccggcaccg tcggcatcaa tgttgcctgg tgcggcatct gcggtaccga
    12181 cctgcacgaa ttcatggaag gcccgatttt cattccgcct tgcggtcatc cgcacccgat
    12241 ttccggcgag tccgcacccg tcacgatggg acacgagttc tccggcgtgg tctatgccgt
    12301 cggcgaaggc gtggacgaca tcaaagtcgg tcaacacgtc gttgtcgagc cttacatcgt
    12361 acacgacgac gtaccgaccg gcccgggcag caactaccac ctctctaaag acatgaactt
    12421 catcggcttg ggcggctgcg gaggtggctt gtctgaaaaa atcgctgtca aacgccgttg
    12481 ggtgcatccg atttccgaca aaatcccgtt ggatcaagcc gctttgatcg aaccgctgtc
    12541 tgtcggacac cacgcctatg tacgcagcgg cgcgaaagaa ggcgatgtcg cattggtcgg
    12601 cggtgcaggc ccgatcggtt tgctgttggc tgccgtgctg aaagccaaag gcatcaaagt
    12661 catcatcacc gagttgagta aagcacgcaa agacaaagcg cgcgaatccg gcgttgccga
    12721 ctacatcctc gacccgtccg aagtcgatgt tgtcgaagaa gtgaaaaaac tgaccaacgg
    12781 cgaaggcgtg gacgtggcat tcgaatgtac cagcgtgaac aaagtgttgg atactttggt
    12841 cgaagcctgc aaacctgccg ccaatttggt tatcgtatcc atctggagcc accctgcaac
    12901 cgtcaacgtc cacagcgtcg tgatgaaaga gctggatgtg cgcggcacga ttgcctactg
    12961 taacgaccac gccgaaacca tcaaactggt cgaagaaggc aaaatcaacc tcgaaccctt
    13021 catcacccag cgcatcaagc tggacgaact ggtttccgaa ggcttcgagc gtctgatcca
    13081 caacaacgaa tccgccgtta aaattattgt gaatccaaac ctgtaagcaa attacatttg
    13141 caacagcaca aatgccgtct gaaacgctca gacggcattt tccaacgaag cgcaacaggc
    13201 gtattgcccg agagcagccg tttagcctat cattccgaac aaacagaaac aaacagaagg
    13261 aaaatcatgg gagattccgt actatccgcc atccaacaaa ccatcaccca gcgaaaatct
    13321 gccaatccgt ccgaatctta cgtcgcacag ctcttgcata agggcgaaga caaaatccta
    13381 aaaaaagtga ttgaagaggc gggcgaagtg ttgatggcat ccaaagacaa aaacccgtcc
    13441 cacctggttt acgaagttgc cgacttatgg tttcacacca tgattcttct gacacaccac
    13501 gacctgaagg cggaagacgt attggacgaa cttgcgcgcc gccaaggttt gtcgggcttg
    13561 gccgaaaaag ccgctcgcac agaatcttga atttatatta aaatccgcac tttcccacat
    13621 tcaatccgtc tgaccgcttt tcagacggca tcggagccgt tatggacaac tgtattttct
    13681 gcaaaatcgc cgccaaagac attccggcgc aaaccgtcta cgaagacggc gaaatggttt
    13741 gtttcaaaga catcaacccc gctgctccgg ttcatctgct gctgattccc aaagtccatt
    13801 tcgattcgtt ggcacacgcc gcgcccgaac atcagcccct tttgggaaaa atgatgctga
    13861 aagttcccga aatcgccaaa gcggcaggac tggcagacgg cttcaaaacc ctgatcaaca
    13921 ccggaaaagg cggcggacaa gaggtcttcc acctgcatat acacatcatg ggcacacccg
    13981 tataaaccgt tatttcacaa tcaaccccta atacttactt aaggatacat catgggcagt
    14041 ttttctctga cgcactggat tatcgtactg attatcgtcg ttttgatatt cggcaccaaa
    14101 aaactgcgca acgtcggcaa agacctcggc ggtgcggttc atgacttcaa acaggggctg
    14161 aacgaaggca cagacggcaa agaagcccaa aaagacgatg taatcgaaca caaaaaagac
    14221 gaagacaaag cgtaatttat gtttgatttc ggtttgggcg agctggtttt tgtcggcatt
    14281 atcgccctga ttgtcctcgg ccccgaacgc ctgcccgagg ccgcccgcac cgccggacgg
    14341 ctcatcggca ggctgcaacg ctttgtcggc agcgtcaaac aggaatttga cactcaaatc
    14401 gaactggaag aactgaggaa ggccaagcag gaattcgaag ctgccgccgc tcaggttcga
    14461 gacagcctca aagaaatcgg tacggatatg gagggtaatc tgcacgacat ttccaatggt
    14521 ctgaagcctt gggaaaaact gcccgaacag cgcacgcctg ctgatttcgg tgtcgatgaa
    14581 aacggcaatc cgcttcccga tgcggcaaac cccgcatcag acggcatttc cgacgttatg
    14641 ccgtccgaac gttcctacac ttccgccgaa acccttggag acgacaggca aaccggcagt
    14701 acagccgaac ccgcggaaac cgaccaagac cgcgcatggc gggaatacct gactgcttct
    14761 gccgccgcac ccgtcgtaca gaccgtcgaa gtcagctata tcgataccgc tgttgaaacc
    14821 cctgttccgc ataccacttc gctgcgtaaa caggcaataa gccgcaaacg cgatttgcgt
    14881 cccaaatccc gcgccaaacc taaattgcgc gtccgtaaat cataaagagg gcaatccggt
    14941 gtccgaaaca caaaacgaac aacccgtcca accgcttgtc gagcatctca tcgagctgcg
    15001 ccgccgcctg atgtggacgg ttgtcggcat cttagtctgc tttttcggcc taatgccgtt
    15061 tgcccaacaa ctctatactt ttatcgccga cccgctgatg gcaaacctgc ccaaagacac
    15121 cagcatgatt gccaccgatg tcatcgcacc gtttttcgtg ccggtcaaag ttaccctgat
    15181 ggcggcattt ttaatttcgc tgccgcatac gctctaccaa atctgggcat ttgtcgcgcc
    15241 cgcactctac caaaacgaaa aacgcctgat tacgccgctc gtcctctcca gcgtcagcct
    15301 gtttttcatc ggcatggcat ttgcctactt tttggttttc cccgtcattt tcaaattcct
    15361 tgccagcgtt acccctgtcg gtgtcaatat ggcgacagac atcgacaaat acctctcctt
    15421 catcttgggg atgtttgtcg cattcggtac aacgtttgaa gtccccattg tcgttatcct
    15481 gttaaccaaa attggtgtgg taacaaccga acagctcaaa cgcgcccgcc cctatgtgat
    15541 tgtcggcgcg tttgtcattg ccgcagtcat cacaccgccc gatgtgattt cacaaaccct
    15601 gcttgccatt ccgctgattc tcttatacga agcaggtatt tggttcggac gctttttcac
    15661 gccacgttca gaacaggatg gcgacataca gccgcctgca acaacctgac actatgccgt
    15721 ccgaacctcc gcctcatacc gcaacagatt aaggaatacc tttgaatacc ctctatttag
    15781 gttcaaacag cccgcgccga atggaaatcc tgacacagtt gggctatcag gtcgtcaagc
    15841 tgcctgccga catcaatgaa acggtcagac agaacgaaga ccctgcccgt tacgttcaaa
    15901 ggatggcaga agaaaaaaac cgaaccgccc tgaccctctt ttgcgaaacc aacggcacaa
    15961 tgcccgattt ccccctgatt accgccgaca cctgcgtcgt ttcagacggc atcatattgg
    16021 gcaaaccccg ctcccaagct gaagcggcaa cctttctaaa cctgctgtcg ggaaccgggc
    16081 ataccgtcct gactgctgtc tgcattcatt atcgcggcaa aacgtcaagc cgcgtccaaa
    16141 ccaaccgcgt cgttttcaag cccctgagtt cggaagaaat ttccgcctat gtccaaagcg
    16201 gcgaaccgat ggacaaagcc ggcgcctacg ccgtacaagg cataggcggc atctttatcc
    16261 aatctatcga aggcagcttc agcggcatta tggggctgcc cgtttatgaa accgtttcga
    16321 tgttacagga tttgggatac cgtaccccac ctttcatccg agcctaaccc gtaaagaccg
    16381 ccgtgaacag acaaaccgct tacctcctta cctctttcag cctgatcgca ctgataatcc
    16441 tgtccctttc ctgggaactg tggattgcac cgttgcgccc gggcggctcg tggctcgcgc
    16501 tcaaagccct ccccctctgt ctgccgcttt caggcatctt gaaaaagaaa atctatacct
    16561 accaatacag ctccatgctg gttctgattt actttgccga agccgtcatg cgcctgttcg
    16621 acgcctatcc cgcagaacag atttgcgccg cgctttccgc agtattcagc atcatcttct
    16681 tcatatcctg cctgtcgttc gtcaaacaat acaaggaaac aaacaatgcc cgctgaaacg
    16741 accgtatccg gcgcgcaccc cgccgccaaa ctgccgattt acatcctgcc ctgcttcctt
    16801 tggataggca tcgtcccctt taccttcgcg ctcaaactga aaccgtcgcc cgacttttac
    16861 cacgatgccg ccgccgcagc cggactgatt gtcctgttgt tcctcacggc aggaaaaaaa
    16921 ctgtttgatg tcaaaatccc cgccatcagc ttccttctgt ttgcaatggc ggcgttttgg
    16981 tatcttcagg cacgcctgat gaacctgatt taccccggta tgaacgacat cgtctcttgg
    17041 attttcatct tgctcgccgt cagcgcgtgg gcctgcaaga gtttggtcgc acacttcgga
    17101 caagaacgca tcgtgaccct gtttgcctgg tcgctgctta tcggctccct gcttcaatcc
    17161 tgcatcgtcg tcatccagtt tgccggctgg gaagacaccc ccctgcttca aaacctcatc
    17221 gtttacagcg ggcaaggcgt aatcggacac atcggacagc gcaacaacct cggacactac
    17281 ctgatgtggg gcatactcgc cgccgcctac ctcaacggac aacgaaaaat cccgcccgcc
    17341 ttgggcgcaa tctgcctgat tatgcagacc gccgttttag gtttggtcaa ctcgcgcacc
    17401 atcttgacct acatagccgc catcgccctc atccttccct tctggtattt ccgttcggac
    17461 aaatccaaca ggcggacgat gctcggcata gccgcagccg tactcctaac cgcgctgttc
    17521 caattttcca tgaacaccat tctggaaacc tttaccggca tccgctacga aactgccgtc
    17581 gaacgcgtcg ccaacggcgg tttcacagac ttgccgcgcc aaatcgaatg gaataaagcc
    17641 cttgccgcct tccagtccgc cccgatattc gggcacggct ggaacagttt tgcccaacaa
    17701 accttcctca tcaatgccga acagcacaac atacacgaca acctcctcag caacttgttc
    17761 acccattccc acaacatcgt cctccaactc cttgcagaga tgggaatcag cggcacgctt
    17821 ctagttgccg caaccctgct gacgggcatt gccgggctgc tgaaacgccc cctgaccccc
    17881 gcatcgcttt tcctaatctg cacgcttgcc gtcagtatgt gccacagtat gctcgaatat
    17941 cctttatggt atgtctattt cctcatcccc ttcggactga tgctcttcct gtcccccgca
    18001 gaggcttcag acggcatcgc cttcaaaaaa gccgccaatc tcggcatact gaccgcctcc
    18061 gccgccatat tcgcaggatt gctgcacttg gactggacat acacccggct ggttaacgcc
    18121 ttttcccccg ccactgacga cagtgccaaa accctcaacc ggaaaatcaa cgagttgcgc
    18181 tatatttccg caaacagtcc gatgctgtcc ttttatgccg acttctccct cgtaaacttc
    18241 gccctgccgg aataccccga aacccagact tgggcggaag aagcaaccct caaatcacta
    18301 aaataccgcc cccactccgc cacctaccgc atcgccctct acctgatgcg gcaaggcaaa
    18361 gttgcagaag caaaacaatg gatgcgggcg acacagtcct attaccccta cctgatgccc
    18421 cgatacgccg acgaaatccg caaactgccc gtatgggcac cgctgctacc cgaactgctc
    18481 aaagactgca aagccttcgc cgccgcgccc ggtcatccgg aagcaaaacc ctgcaaatga
    18541 cccgcgccgg cggatgcgga taccgcccga aatgtaaagc tccatgcaag acattgcaaa
    18601 aaacagcaaa ccggtaggga aatacgctat caaaaaacat tgcagccgtg ttaagataaa
    18661 ccgtcaaaca atcttttcac gccccgcccg aaacagggtc ggggcatacc cttacgaaaa
    18721 ggaaacacca tgagccgcgt attactcgta gatgacgatg ccctgctaac cgaactgctg
    18781 accgaatacc tgagcgccga aggtctgaac gtccgcagcg ttcccgacgg ggaagcaggc
    18841 gtacaggaaa tcctgagcgg gcagtacgat gtagtcgtat tggattccat gatgcccaaa
    18901 atgaacggct tggatgtctt gaaaaacgta cgcgcccgaa gcaccgtccc catcatcatg
    18961 ctgaccgcca aaggcgacga catcgaccga atcatcggct tggaaatggg cgcggacgac
    19021 tatgtcccca aaccctgcac accacgcgaa ctcttggcac gcatcaatgc catcctgcgc
    19081 cgcgcacaac acagcggcga acagaacaac gcacccaaca gcatctccgt cagcgatgtc
    19141 gtcctgtacc ccgccaaacg ccaggcatcc gtcaaagaca tgccgctcga actgaccagc
    19201 accgaattca acctgctcga agtcctgatg cgccatgccg gacaggtagt cagcaaagaa
    19261 accctgtccg tcgaagcact cgaccgcaag ctggcaaaat tcgaccgcag tatcgacgta
    19321 cacatctcca gcatccgcca caagttgggc gatgcctctc tgattcaaac cgtacgcggc
    19381 ttgggctacc tgtttgtcaa aaactgaagc aacgggcaaa tgaaactgtt ccaacgcatt
    19441 ttcgccacat tttgcgcagt tatcgtctgt gcaatctttg tggcgagttt ttctttttgg
    19501 ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac gccgcaccat cgaaaccact
    19561 ttgatgggca gcatcatttc cgcattccgg gcacgcgggg acgcgggcgc gcgcgaaatc
    19621 ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt acgttataca gggcgacgag
    19681 aaaaaagata tcctgaaccg gtatatcgac agctacacca tcgaacgcgc ccggcttttc
    19741 gccgccggac acccgcattc caacctcgtc catatcgaat acgaccgctt cggcgaagaa
    19801 tacctgttct tcaccaaaga ctgggacaaa ctccaagccc gccgcctgcc cagccccctg
    19861 ttgatccccg gtctgccgct cgccccgatt tggcacgaac tcatcatatt gtccttcatc
    19921 atcgtcgtcg gactgctgat ggcgtacatc ctcgccggca atatcgccaa acccatcaga
    19981 atcttaggca acggcatgga cagggtggca aacggagaac ttgaaacccg tatctcccaa
    20041 caggtcgacg accgcgacga cgaattgtcc catcttgcca tccaattcga caaaatggtg
    20101 gaaaaacttg aaaaactcgt tgccaaagaa cgccacctgc tccatcacgt ctcccatgaa
    20161 atgcgttctc cccttgcgcg catgcaggca attgtcggac tgattcaggc gcagccccaa
    20221 aaacaggagc aatatctcaa acggctggaa ggcgaactga cccgcatgga tacgctggcc
    20281 ggggaactgt taaccctgtc ccgtctcgaa acttccaata tggctttgga aaaagaaagc
    20341 ctgaaactcc tgcccttcct gggcaacctg gtagaagaca atcaaagcat tgcccagaaa
    20401 aacggacaaa cggttaccct gtctgccgac ggaaaaatcc ccgaaaatac aaccatcctt
    20461 gccaacgaaa gctacctgta ccgcgccttc gacaacgtca tccgcaacgc cgtcaactac
    20521 agtcccgaag gcagcaccat cctgatcaac atcggacaag accacaaaca ctggataatc
    20581 gacgttaccg acaacggccc cggcgtggac gaaatgcagc tcccgcacat cttcaccgct
    20641 ttctaccgtg cagactccag tgcgaacaaa cccggaacag gactggggct tgcattgacc
    20701 caacatatta ttgaacagca ctgcggaaaa atcatcgccg aaaacatcaa accgaacggt
    20761 ctgcggatgc gcttcatcct gcccaagaaa aaaaccggtt ccaaaacaga aaaaagtgcg
    20821 aactgaccat aatgccgtct gaaccggctt cagacggcat tgcacaaaca gttaccccaa
    20881 aacaaacaca cagcgggtgc atctccaaca atcacgcaca taccgtccgc aaaggaatat
    20941 atcatgtcgg cacaaaccga tccgggctac tttttcatgc ccaaccacat tattctgata
    21001 ggcgcgagcg aacgtccgta cagcctgggc gaacgcgtac tcagcaacct gctgagtacg
    21061 ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc acaccatagc cgggcttccc
    21121 gcctacacca gcctcaacaa aatccccggc aatgcagacc tgattattgc cgttacccta
    21181 cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa agcagctccg acacatcatc
    21241 ctcatacagg actgggacag cctgtctgcc gcagaactgc acaccgccga aactgccatc
    21301 cgcaaacacc acggcaacgg acttaacatc accgcctgca ccaccgcagg catccaactg
    21361 ccctcactcg gactcaacat cagtacccaa gacggatacg ccgcaggcca taccgccata
    21421 ctgaccggca atgccgccgt cagccgtcaa atcgacaaca tcctgaacaa actccgtcaa
    21481 ggcacatccc gccacatcag cctgcatccc ggcatcagcc ccatcacatc cgccgattgg
    21541 ctcaaccgct tcggacacag cctgcacacc aaaaccgccg tcctacacca caaccctgaa
    21601 gaagaccagc gcaaactgtt cagcgcaatc cgccaattta cccgccatac gccgctgatt
    21661 ctccacatca cctgccgcac gacagaaacc gaccgtgccg tactgcacag cctcgcccga
    21721 cactgcaact tcctcatcag tttcaacgcc gacgacctcg aagccgcact gcgcgcccaa
    21781 ctgtccgacc ttcctcccct gtcccgactc gacatcctgt ccgacacgcc tgccgaatgg
    21841 ctgaacgcgc acgcgccaaa aaacctcgcc ctccactttc ccaaccttcc ccaccacatc
    21901 cgcaacggac acctgaccgg cacacccaca ccctcaatct gccacgacat cgcctcacgt
    21961 cagcttgccc gccccgacac ccaagccgtc ctaaccatcc tcagtccctc cggacacgag
    22021 gattacaaaa aaacagcacg cgcccttatc cgcctgtccg aacagaccgc caaacccctg
    22081 ctcgtcagca gccccttttc agacggcata acacatttcg acacccccac tcaggcaatc
    22141 cgcacccttt cctaccgcaa caccgccgcc gccctgaaac aggcacagct cgacattgca
    22201 ccgccgcagc catgccgtct gaaaacaccg caaccccaaa acatcaaaaa agcccttgca
    22261 gcggcaaacc cctccctgct cgccgaagcc ctgcacctcc ccccctaccg gcacaccacc
    22321 cataacgccg tacaattcca attcgacagc caccccctct atggcgacat cctgaccgca
    22381 cgctgcaacg gacaaaccac tgccgtactc ccgccgttta ccacgctcga cagccgccac
    22441 cttgcccgct ttgccgaact cgacggcaca caaaccctcg accagttcct gcacacactg
    22501 accatcattc ccgaataccg ccaacacatt ctcggcatca ccctcaacct aaacggcgga
    22561 caatacagca gcgatttcat cttaaagaca cccgaaacac acgacacgcc caaacgcaag
    22621 aacacaggca aagccgccca aaccctcgaa catgccgccg caaaaatgca gagtgccgcc
    22681 gcatacctga aacacaaaaa cccgacagcc gccgaatttc tccgccacac aagcgaagcc
    22741 gccgcagaac tgctcggcag caaaaccgaa accggagcag ccgtacccaa cgtacttgcc
    22801 ccctatcccg cagcataccc caaaacactg tccctaagaa acaacacgac cgttaccatt
    22861 acccccattt tgcccgaaga cgcagaagcc aaacagcagt tcgtccgcag cctcggtccc
    22921 gaagcacggt acacacgctt catgacccac accaacgaac tgcccgcagc cacgttggca
    22981 cgcctgtgca accccgatta ccactgtgaa gccgcatgga cggcaaagga tgccgacagc
    23041 aacatcgtcg ccgtcgtccg ccacagccgc ctgaatcgca acgaatgcga attcggcatc
    23101 acactggcgg aacatatgcg cggcagcgga ctggcacaga aaatgatgga actcatcatc
    23161 caaaccgccg cacagcaagg ctaccggact atgagtgccg acattctcaa aaccaatacc
    23221 cccatgatca aacttgctga aaaatcagga tttaccctca aggaatcgga caccgaaaaa
    23281 aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa caacagaaaa aacaaataaa
    23341 aacttgcgca ccgaccacaa aataacctaa aatcggcagt ttccatatat ccgattttct
    23401 caaaaggact caaaatggta gttatccgtt tggcacgcgg cggctcgaaa caccgcccct
    23461 tctacaacgt catcgttact gactcacgca gccgccgcga cggccgcttc atcgaacgcg
    23521 taggcttcta caaccccgta gccaacgaaa aacaagagcg cgtccgcctc aatgcagacc
    23581 gcctgaacca ctggattgca caaggcgcgc aagtcagcga ctccgttgca aaactgatta
    23641 aagaacaaaa agccgcctaa tccgcatttg ccgccatgac agacactcaa aaccgggtag
    23701 ccatgggcta catcaaaggc gtattcggca taaaaggctg gttgaaaatt gccgccaaca
    23761 ccgaatattc cgacagcctt ttggactacc ccgagtggca tttggtcaag gacggcaaaa
    23821 ccatcagcgt tacccttgaa gccggaaaag tcgtcaacgg cgaactccaa gtcaaattcg
    23881 aaggcataaa cgaccgcgac ttggcattct cattgcgcgg ttacaccatc gaaatacccc
    23941 gtgaagcatt cgccccgaca gaagaagacg aatactactg gacagacttg gtcggcatga
    24001 ccgtcgtcaa caaagaccat accgttttag gcaaggtaag caacctgatg gaaaccggcg
    24061 caaacgacgt attgatgatt gacggagaac acgggcagat tctgattccg ttcgtttccc
    24121 aatatatcga aaccgtcgat accggcagca agattattac tgccgactgg ggtttggact
    24181 actgatgctt atccaggcag ttaccatttt ccccgaaatg ttcgacagca ttacccgcta
    24241 cggcgtaacg ggacgcgcga acagacaggg aatctggcag tttgaagcag tcaatccccg
    24301 aaagtttgcc gacaacagat tgggctacat cgacgaccgc ccgttcggcg gcggcccggg
    24361 aatgattatg atggctccgc cgcttcatgc agcaatagaa cacgccaaag cacaatcttc
    24421 ccaagctgca aaagtcattt acctcagccc ccaaggaaaa ccgctgacac acccaaaagc
    24481 ggcagaactg gcagaacttc cgcatctgat tctgctgtgc ggacgctatg agggaataga
    24541 cgaaagactg ctgcaaacca gcgtcgatga agaaatcagc atcggagact tcgttgtttc
    24601 cggcggagag cttcccgcca tgatgctgat ggatgcggta ttgaggctcg tacccggcgt
    24661 attgggcgat atgcagtctg ccgaacaaga ttcgttctca agcggcattt tggactgccc
    24721 ccactacacc aaacccttag aatttcaagg catggctgtt ccggaagtat tacgttccgg
    24781 caatcatggc ttgatagcgg aatggcggtt ggaacaatcg ctgcgccgca ccttggagcg
    24841 cagacccgat cttttggaaa agcgcgtttt aatcccaaag gaatcccgcc tcttagaaac
    24901 catccggcaa gagcaacggg aaatccaatc ataatttagg aaaaaaacaa tgaacctgat
    24961 tcaacagctc gagcaagaag aaattgcccg cctgaataaa gaaatccccg aattcgcacc
    25021 gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt acccgcagcc gtctgcaagc
    25081 ctacgaaggc gtggttattg cccgtcgcaa ccgtggtctg aacagcaact tcatcgtccg
    25141 caaaatctcc agcggcgaag gtgttgaacg tactttccaa ctgtactctc cgaccgtcga
    25201 aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc aaactgtact acctgcgcgg
    25261 cctgaccggt aaagctgcac gcatcaaaga aaaactgcct gcacgcaaag gttgattcaa
    25321 accgttttcc ccaatgccgt ctgaaccttc tgacggcatt tcttattgcc gcaacatccg
    25381 caacggaaca gcaccaagaa gcacacccct tccggcacat cccgaatccc cgtccgaacc
    25441 gaacaaaatt tacagaccaa acgttataca gtatcatatc agccatataa taataaccgc
    25501 tttaccgctt tccctttcaa aaccgcaaat catcatgaca ccttcccttt tactatcagg
    25561 attgaccttc cgcctcgtcc ttgccctgat tgccgtatcc cttttatggg gcgtttactt
    25621 ttgggcagta tccgcatgag catcattgtc gaaaacctga ccgtcagcta ccgccgccgt
    25681 cctgccgtac accacgttga cattactttt gaagaacata gtatgtgggc ggttttcggt
    25741 cccaacggcg cagggaaatc cacctttctc aaatccttga tgggattgca gcctatcgat
    25801 acaggcagca tccggctgga tggattgagc cgccaagaca tcgcctacct tccccagcag
    25861 tccgatatcg accgctccca acctatgact gttttcgact tggcggcaat ggggctttgg
    25921 tatgaaatcg gctttttcaa agggataaat accgctcaaa aacaacgcgt tcacgaagcc
    25981 ttggagcgtg taggaatgcg gcagtttgca cgccgccaaa tcgcccacct ctcaaacgga
    26041 caattccaac gcgtcctttt tgcccgaatg ctggttcaaa atgcaaaatt cctgcttttg
    26101 gacgaacctt tcaacgccgt tgatgcacgg acgacctacg aacttctcga cgtattgcag
    26161 aaatgccatt gcggcggaca cgctatcatt gccgtactgc acgattacga acaagtccgt
    26221 gcctactttc ccagtaccct gctgctcgcc cgagaaaaaa ttgcggcagg cgcaaccgag
    26281 accattctga cagaacccta cctcgcccaa gccaacgcca aaatgcagca acaggaaagc
    26341 cccgactggt gcgcctcata aatactgccc gaaaccacgc cccacaccag acaccatgaa
    26401 tctctacgac ctgctccttg ccccctttgc agaattcgac tttatgcgct acgccctcgc
    26461 atccgtcttc tgcctgtccc tcagtgccgc acccgtcggc gtattcctcg tcatgcgccg
    26521 tatgagcctg ataggcgacg cattgagcca cgccgtcctg cccggtgccg ccgtcggcta
    26581 catgtttgcc ggcttgagcc tgcccgccat gggtttgggc ggcgtagccg caggcatgct
    26641 gatggcactg cttgccggac tcgtcagccg cttcaccacc ctgaaagaag atgccaactt
    26701 tgctgccttt tatctcagca gcctcgccat cggcgtagtc ctcgtcagca aaaacgggag
    26761 cagcgtcgat ttgctccacc tccttttcgg ctctgtactt gccgtcgata ttcctgccct
    26821 gcagctcatc gccgccgtct ccagcctcac gctcattacc cttgccgtca tctaccgccc
    26881 gctcgtactc gaaagcatcg accccctgtt tctcaaatcc gtcggcggca aaggcgggct
    26941 ttggcacgtc ctctttctcg tcctagtcgt catgaacctc gtatccggct ttcaagccct
    27001 cggcacactc atgtccgtcg gactcatgat gctgccagcc attaccgccc gcctgtgggc
    27061 gaagcacatg ggcgcactca tcctcctatc cgttctgaca gccctgctgt gcggcttgag
    27121 cggactgctc atttcctacc acatcgaaat tccctccggt cccgccatca tcctctgttg
    27181 cagcgtcctt tatctctttt ccgtcatact cggcaaagaa ggcggcattc tgaccaaatg
    27241 gctcaaaaac caccgccacc acaccacctg accccgcaag ccgcttgaac aaacaggcag
    27301 cttgttttac cataacagat gttacgttat atcacaggag aacagttatg aaacacctca
    27361 aactcaccct tattgccgca ttgctaacca ccgccgcaac tgccgccccc ctgcccgtcg
    27421 taaccagctt cagcatttta ggcgacgttg ccaagcaaat cggcggagag cgcgtatcca
    27481 tacaaagttt ggtcggagcc aaccaagata ctcatgccta tcacatgacc agtagcgaca
    27541 ttaaaaaaat ccgcagtgca aaactcgtcc tgctcaacgg cttaggactt gaagctgccg
    27601 atgtacaacg tgccgtcaaa cagagcaaag tatcctatgc cgaagcgacc aaaggcatcc
    27661 aacccctcaa agccgaagaa gaaggcggac accatcatga ccacgaccat gaccacgaag
    27721 gacaccacca tgaccacggc gaatttgacc cgcacgtctg gaacgacccc gtccttatgt
    27781 ccgcctatgc ccaaaacgtc gccgaagccc tgataaaggc cgaccccgaa ggcaaagttt
    27841 attatcaaca acgcttgggc aactaccaaa tgcagcttaa aaaactgcac agcgacgcac
    27901 aagccgcatt taacgccgtc cctgccgcca aacgcaaagt cctgaccggg cacgatgcct
    27961 tttcctacat gggcaaacgt taccatattg aattcatcgc cccgcaaggt gtgagcagcg
    28021 aagccgagcc ttcagccaaa caagtcgccg ccatcatccg acaaatcaaa cgcgaaggca
    28081 tcaaagccgt ctttaccgaa aacatcaagg acacccgtat ggttgaccgt atcgccaaag
    28141 aaaccggtgt caacgtcagc ggcaaactgt attccgacgc actcggcaac gcgcccgcag
    28201 acacctacat cggcatgtac cgccacaaca tcaaagcctt gatcaacgcg atgaagcaat
    28261 aaaaccgttt gattggtttg aatttgaaaa ggcaaaaggc cgtctga
//
LOCUS       DAXIHS010000019        27931 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXIHS010000019 DAXIHS010000000
VERSION     DAXIHS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.019"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(63..1991)
                     /gene="dnaK"
                     /locus_tag="ACFOF2_000700"
     CDS             complement(63..1991)
                     /gene="dnaK"
                     /locus_tag="ACFOF2_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC7107056.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2199..2471)
                     /locus_tag="ACFOF2_000701"
     CDS             complement(2199..2471)
                     /locus_tag="ACFOF2_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107057.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2659..3345
                     /locus_tag="ACFOF2_000702"
     CDS             2659..3345
                     /locus_tag="ACFOF2_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC7107058.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3477..3815
                     /gene="erpA"
                     /locus_tag="ACFOF2_000703"
     CDS             3477..3815
                     /gene="erpA"
                     /locus_tag="ACFOF2_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC7107059.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3832..3978
                     /locus_tag="ACFOF2_000704"
     CDS             3832..3978
                     /locus_tag="ACFOF2_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107060.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            3975..4439
                     /locus_tag="ACFOF2_000705"
     CDS             3975..4439
                     /locus_tag="ACFOF2_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC7107061.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            4474..5985
                     /gene="ubiB"
                     /locus_tag="ACFOF2_000706"
     CDS             4474..5985
                     /gene="ubiB"
                     /locus_tag="ACFOF2_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC7107062.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6342..7160)
                     /gene="cysE"
                     /locus_tag="ACFOF2_000707"
     CDS             complement(6342..7160)
                     /gene="cysE"
                     /locus_tag="ACFOF2_000707"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC7107063.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7344..7922
                     /gene="grpE"
                     /locus_tag="ACFOF2_000708"
     CDS             7344..7922
                     /gene="grpE"
                     /locus_tag="ACFOF2_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC7107064.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8199..8414)
                     /gene="nqrM"
                     /locus_tag="ACFOF2_000709"
     CDS             complement(8199..8414)
                     /gene="nqrM"
                     /locus_tag="ACFOF2_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC7107065.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8440..9495)
                     /locus_tag="ACFOF2_000710"
     CDS             complement(8440..9495)
                     /locus_tag="ACFOF2_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC7107066.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9648..10865)
                     /gene="nqrF"
                     /locus_tag="ACFOF2_000711"
     CDS             complement(9648..10865)
                     /gene="nqrF"
                     /locus_tag="ACFOF2_000711"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC7107067.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(10879..11472)
                     /gene="nqrE"
                     /locus_tag="ACFOF2_000712"
     CDS             complement(10879..11472)
                     /gene="nqrE"
                     /locus_tag="ACFOF2_000712"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC7107068.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(11476..12102)
                     /locus_tag="ACFOF2_000713"
     CDS             complement(11476..12102)
                     /locus_tag="ACFOF2_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC7107069.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(12102..12878)
                     /locus_tag="ACFOF2_000714"
     CDS             complement(12102..12878)
                     /locus_tag="ACFOF2_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC7107070.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(12871..14103)
                     /locus_tag="ACFOF2_000715"
     CDS             complement(12871..14103)
                     /locus_tag="ACFOF2_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC7107071.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFGALTPDLLQQNIANDWHYTLAD
                     ALGINMSSEASVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(14106..15449)
                     /locus_tag="ACFOF2_000716"
     CDS             complement(14106..15449)
                     /locus_tag="ACFOF2_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC7107072.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAAPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(15797..17218)
                     /locus_tag="ACFOF2_000717"
     CDS             complement(15797..17218)
                     /locus_tag="ACFOF2_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC7107073.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(17233..17613)
                     /locus_tag="ACFOF2_000718"
     CDS             complement(17233..17613)
                     /locus_tag="ACFOF2_000718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HFC7107074.1"
                     /translation="MSPYGCGAGPNLECRTERDKGKQTGGKPVSLQIYVKINSFFVFD
                     RLFIKDIYLFVLNKIIYQKEHIFPSTAIRLPAAFRFGKLVYPDYLLYNQSENFFQKQT
                     GSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(17576..17935)
                     /locus_tag="ACFOF2_000719"
     CDS             complement(17576..17935)
                     /locus_tag="ACFOF2_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC7107075.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(17932..18537)
                     /locus_tag="ACFOF2_000720"
     CDS             complement(17932..18537)
                     /locus_tag="ACFOF2_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC7107076.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGILQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(18584..19066)
                     /locus_tag="ACFOF2_000721"
     CDS             complement(18584..19066)
                     /locus_tag="ACFOF2_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC7107077.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      19270..19367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19578..20678
                     /gene="gcvT"
                     /locus_tag="ACFOF2_000722"
     CDS             19578..20678
                     /gene="gcvT"
                     /locus_tag="ACFOF2_000722"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC7107078.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYCIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            20839..21225
                     /gene="gcvH"
                     /locus_tag="ACFOF2_000723"
     CDS             20839..21225
                     /gene="gcvH"
                     /locus_tag="ACFOF2_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC7107079.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            21372..22619
                     /gene="hemA"
                     /locus_tag="ACFOF2_000724"
     CDS             21372..22619
                     /gene="hemA"
                     /locus_tag="ACFOF2_000724"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC7107080.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            22947..23468
                     /locus_tag="ACFOF2_000725"
     CDS             22947..23468
                     /locus_tag="ACFOF2_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC7107081.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDVVNTRGYSSKPIDTLMALA
                     KDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFTSTNRLRLKGLPT
                     VLQPSKETGAGVP"
     gene            23357..24556
                     /locus_tag="ACFOF2_000726"
     CDS             23357..24556
                     /locus_tag="ACFOF2_000726"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC7107082.1"
                     /translation="MASGVYQTKLHIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDGLLKEAETRQLEWGR
                     AENGSLN"
     gene            24614..25228
                     /locus_tag="ACFOF2_000727"
                     /pseudo
     CDS             24614..25228
                     /locus_tag="ACFOF2_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            25323..25454
                     /locus_tag="ACFOF2_000728"
                     /pseudo
     CDS             25323..25454
                     /locus_tag="ACFOF2_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            25451..25945
                     /locus_tag="ACFOF2_000729"
     CDS             25451..25945
                     /locus_tag="ACFOF2_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC7107083.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(26033..27694)
                     /locus_tag="ACFOF2_000730"
     CDS             complement(26033..27694)
                     /locus_tag="ACFOF2_000730"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC7107084.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSETGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTKKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGMKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMIAAEAVFPLLEHLEEAEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         6619 a         6790 c         7623 g         6899 t
ORIGIN      
        1 gttggaatat agggttaaaa tagggctctt ttgggcttat tcaaattagg gacacgccct
       61 aattattttt tgtcgtcttt tacttcttca aagtcggcat ctacgacatc atcgtctttc
      121 tttgcagaag cattggcttg ttcgctttcg cctgcttggg cttcagcttg tgcttgagcg
      181 taaaccattt cccccagttt ttggctggct gcgcccagcg cctcggtttt ggcatcgata
      241 gcggctttgt cgtcgccttt aactgcttct tcggcttctt tcagcgcggc ttcgattttt
      301 tctttctcgg ctgcgtcgag tttgtcgccg tagtcggcca aagatttttt cacagagtga
      361 atcagggctt cggcttggtt gcgggaagcg accaattcag tcagtttttt atcttcctcg
      421 gcattggctt cggcatcttt caccatgcgt tcgatttctt cttcgctcaa acctgaagaa
      481 ccttggatgg tgatgttggc tgctttgcca gtgcctttgt ctttggcgga aacgtgcagg
      541 ataccgttgg cgtcaatatc gaaggtgact tcgatttgcg gcataccgcg cggtgcgggt
      601 gcgatgtcgc ccaagttgaa ctgacccaaa gatttgttgg cagaagcgcg ttcgcgttcg
      661 ccttgcagta cgtggatggt tactgcgctt tggttgtctt cggcggtaga gaacacttgc
      721 gacgctttgg tcgggatggt ggtgttcttt tgaatcagct tggtcatcac gccgcccatg
      781 gtttcgatac ccaaggacag aggggttacg tccagcagca atacgtcgct gcggccgccg
      841 cttaacactt cgccttgaat cgctgcgcct acggcgacag cttcgtcagg gttcacgtct
      901 ttgcgcggct ctttgccgaa gaagtcttta acggcttctt gtactttcgg catacggctt
      961 tgaccaccga ccaaaatcac gtcgtcgatg tcgccagtgc tcagaccggc atctttcaat
     1021 gcggtgcggc aaggctcgat agagcgggca atcaggtctt caactaggct ttcgaatttg
     1081 gcgcgggtaa ttttcatcgc caagtgtttc gggccggttg cgtccatggt gatgtacggc
     1141 aggttgattt cggtttgctg gccgctggac aattcgattt tggctttttc ggcagcttct
     1201 ttcagacgtt gcagagccat gacgtcttgt ttcaaatcaa tgccttgttc ttttttaaac
     1261 tcgtcaatga tgtagtcgat caagcgttgg tcgaagtctt caccgcccaa gaaagtatcg
     1321 ccgttggtag ccaatacttc aaattgtttg tcgccgtcga ggttggcgat ttcgatgatg
     1381 gaaatatcga aagtaccgcc gcccaagtca tatacggcta ctttgcggtc tttgttgtcg
     1441 cctttgtcca taccgaatgc caaagcggct gcggtcggct cgttgatgat gcgttttacg
     1501 tccaaaccgg cgatacggcc tgcgtctttg gtggcttgac gttggctgtc gttgaagtag
     1561 gccggaacgg taatcacggc ttcggttact ttttcgccca agtaagcttc ggcggcttct
     1621 ttcattttac gcaggacttc tgcggaaatt tgaggaggag acagctcttt gccttgtgct
     1681 tttacccatg cgtcgccgtt gttggctttg atgatttcga aaggcataga ttcgatgtcg
     1741 cgttggactt ctttgtcttc aaatttgtgg ccgatcaaac gtttggcggc gtaaatagtg
     1801 tttttggcgt tggttaccgc ttggcgtttg gcaggcgcgc cgacgaggat ttcgccgccg
     1861 tccaaataag cgataacgga cggcgtggtg cgtgcgcctt ctgcgttttc gataactttg
     1921 gtttgaccgt tttcggaaat ggccaaacaa gagttggttg tacctaagtc gataccgatt
     1981 acttttgcca tgtggataat cctatttgat tttgcttatt ttgagaaata tgttggaaca
     2041 ttttgtcccg atgggctgta aatagggcgg gcggcgggct gtttcaagct acagcatggc
     2101 tataagtata taactttatg aatatattgg ttttatattt gatttaatac atttggctcc
     2161 aatgcattca agcataatat ttcaaatggc aggcaggttt attcgtagac gatgccggcg
     2221 agcatttcct gttcgttcaa gttgccgtac tctttttccc agtcgtgtga agactcgatg
     2281 atgtcgcatt ctttggaaag ggagacttgt tctgcatcca tatctttggc gttcagtatg
     2341 ttgaattgtt cgcacaggga tgcggataaa gtgatgtcgg gctgtttggc ttcagaacgg
     2401 ttttcttgga aggcaaagca gaatgcggta aatgccgcag tatagataag atatttgccg
     2461 gttttcttca tttttctatc ctttttctgt caattcagga ttaaacctat ggaaaaatct
     2521 gaaaaattat gtattaagta agaaaaatca taatttaaat ttagtttatc ataattgttc
     2581 cgttttttgg atagctaagg taaaatatat ttcatgttta ctttagatga ttgaatgaag
     2641 gggagtggaa ggatatttat ggaaaccttt aaagacagac tggttttttt atggaaaagc
     2701 gaagcgaggc aggcaaaaat cgcatccgat attgaaatga cgattgcggg cttcagcagg
     2761 atatggaatg aaggcggtct gccaaagtct gaaacattga aaaaaatcaa gcagttgaag
     2821 gggtgtagta tcgattggct gctgaccggg gagggtaatc cgtttccgga tgaagcccca
     2881 aaaaaatccc ttgcttacga tactttgggc aatgaagtcg atacggacga gtttgtcttc
     2941 gtgccgagat atgatattcg ggcggctgcg ggatacgggc agtttgtcga tcatgaggaa
     3001 ccggtattta caatggcgtt cagacggcat tggattgaga attatgttac ccgcgatacg
     3061 aaaaacctgt ctgtaatttc cgtcaagggg gattcgatgg agggggtttt gaatgacggc
     3121 gattcgattt tggtcaatca tggtgaaaat acgccgaggg acggtctgta tgtgttgcgg
     3181 attaatgaaa atctgctggt taaacgttta cagattgtac cgggcgggat tatcaatgtg
     3241 atttctgcaa acgaggctta tcctgctttt gaaatcaatt tgaacgattt gaccgatgat
     3301 gtggagatta tcgggcgtgt cgagtggttc ggcaggacga tttgagtttg gggcttgaaa
     3361 ttgcaggcgg tcaaacttat ctattggaac aattcctttt tcaaaggcga agcctgcttg
     3421 cctttgaagg gggtttgaga gagaatgcag aaaatattat attaaggaat aacaccatgt
     3481 cggatgaaag ccctattatt tttactgaca gctgctgtgc caaagttgcc gatttgattg
     3541 ccgaagaaaa caatcccgat ttgaaattgc gggtttttgt caatggcggc ggctgttcgg
     3601 gtttccagta cggatttact tttgacgaaa tcaaaaacga cgacgatttt gaaattgaga
     3661 aaaacggttt ggtctttttg gtcgatccga tgagctatca atatctggtc ggtgcggaaa
     3721 tcgactatac ggaaagtttg cagggttcgc aattcgtcat ccgcaatccg aatgcggaaa
     3781 caacctgcgg ttgcggatcg tcgttttccg tatgaccgct tggtttgtgt gatgccgtct
     3841 gaacgttcag acggcatttt tacttttaga aaatatatta tcgggatgaa ttcacatata
     3901 atccgattgt ttgaagatga atcgggtttc ccgaaaggaa cgggcggaac ggtatcaggc
     3961 gtatttgttc ccttatgatt gagatgagta aagattaccg aaacgatttg tacgatgtat
     4021 atgtttctta cccgccccaa gtggatcgcg ggcttatccg ggagtgcctt aaggagaatc
     4081 tcggcgagga aaaggcggaa ggattgatcg aatcgctcga ttccaaacct caagtgctgg
     4141 ttgaggaaaa atgcacttgg gcgaaacggg aagagttgca tgattatttc agctatttgg
     4201 gtttggatat tattacccgg agatatatgg agttggaaac ggtcgtgccg ccggaggaag
     4261 gggagggcga aggagaaggc gcggatgggg aaatgcccga atatcttgaa cttcacggcg
     4321 ggcgggaaga tgatatttcc gcaccttcgc aacccgaacc gccgtcccgc aatatcaaac
     4381 tgctggtttt cgggctgctg attgcctttt tgggctatct gctcggtaag attttttgat
     4441 tgtccgataa atgctgtatt cgggatttta tatatgaaat ggttgaaacg cctgacggtt
     4501 attgtcggga ctttttaccg ctatcggctg gcaggtctgt gtgcttcgct gatgggtagc
     4561 ggttggatat gcgctctgct gaaaatgatg ccgcagtcgt ccaaattgaa aaacgaaccg
     4621 cctgctgtcc gtctgcgcct tgccttggaa agcctggggc cgattttcat caagttcggg
     4681 caggttttgt ccacacgccc cgatttgatt ccgcacgatt acgccgtcga actggcaaag
     4741 ctgcaagaca aagtgccgcc ttttgacgcg cggctttcgc gtgaacaaat cgaaaaatcg
     4801 ttgggtcagt ccatcgaaaa gctgtatgcg gaatttgaaa ccgagcccat cgccagcgcg
     4861 tccatcgccc aagtacacaa agcccgcctg cattcgggcg aacgagtggc ggttaaagtt
     4921 ttgcgcccca accttttgcc ccttatcgaa caggatttgt cgctgatgcg tttcggcgcg
     4981 gcctgggtcg aacgcttgtt ttcagacggc aggcgtctga agccgctcga agtggtggcg
     5041 gagttcgaca aatacctgca cgacgagttg gatttgatgc gcgaagccgc caatgccgga
     5101 cagctcgggc gcaatttcca aaacagcaat atgctgattg tgcccaaggt gttttacgac
     5161 tactgcacca gcgacgtgct gacaatcgaa tggatggacg gcacgccggt tgccgacatc
     5221 gccaaactca aagcagacgg catcgatttg cacaaactcg ccgattacgg cgtggaaatc
     5281 tttttcacgc aggtcttccg cgacggcttt ttccacgcgg atatgcaccc cggcaatatt
     5341 ttggttgccg ccgacaaccg ctacatcgcc ctcgatttcg gcatcgtcgg cacgctgacc
     5401 gattacgaca aacgttatct cgccatcaat ttcctcgcct ttttcaaccg cgattaccgg
     5461 cgcgtcgcca ccgcccacat cgaatcgggc tgggtgcctg ccgacacgcg tgcggaagaa
     5521 ttggaagccg ccgtccgcgc cgtgtgcgaa ccggtgttca acaaaccgat ttcgcagatt
     5581 tccttcggct tggtgctgat gcgcctgttt gaagtcagcc gccgcttcaa tgtcgaaatc
     5641 caaccgcagc ttgtgctgct gcaaaaaacg ctgctcaaca tcgaaggctt gggccgccag
     5701 ctcgatcccg atttggactt gtggaaaacc gccaaaccgt ttttggtgaa atggatgaac
     5761 gggcaggtcg gccctaaagc cctttggcgc aacctcaaaa acgaagcccc cgactgggcg
     5821 caaatcatcc cttcattgcc gcgcaaaatc agtgcgttga ttgatgaaaa ccgccagcag
     5881 gaaatgcgtg atgcctatat ccatttggtc aaagtgcagc agcggcaaag cctgtggctg
     5941 gctgtgattg cggttgtttt gctgctgatt ttgcttttga aataggcttt gtccgaatca
     6001 tcgcccgact ccgcccgttt ataaggaaat cggttatagt ggattaacaa aaaccagtac
     6061 ggcgttgtct cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga
     6121 atcgattccg tactatccgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     6181 tccactatat ttccggttgc gtgggaatcg ggtgtattga ataaaaggca ttttgtccga
     6241 ctggcaagtg ccgacatcgg cggcatatca aggcgcaggc ttgaagcggg caatgtcgtc
     6301 tgaagcccgt ttggcgtttc agacggcatt ggtgcggata ttcaaatcat aaagtcgatt
     6361 tcggtaaact ggatattttg atccatatcc gccgacggtg ttttgagcga tcgcgccacg
     6421 ggtttggcgg gtacgccgac aaccgtgatg gacggcggca cgtctgaaac cacgacgctg
     6481 cccgccccga ttttggcatt gctgccgatg cggatattgc ccaatatcga ggcgtttgcg
     6541 ccgatcatca cgccgtcgcc gattttaggg tggcggtcgc cgccttcttt gcccgaaccg
     6601 ccgagcgtta cgccgtgcaa aatcgaaata ttgttgccca acacggcggt ttcgccggca
     6661 acaaagccgg tggcgtggtc gagcatcagc ccgtatccga aacgggcggc gggatggatg
     6721 tccacgccga atacttcgga catacggttt tgcaggaaat acgccagcgt tttgcgcccg
     6781 tcgagataca gccgatggtt gatgcggtgt gcctgaatcg cgtggaagcc tttgaaatat
     6841 aaaagcggca gcgaatattc gtcgcaggcg ggatcgcgtt cgtagatggc ttttaagtct
     6901 gcttcgacgc atttgccgat ttgggtgtcg ctgcccaacg cctgctggta gatttcaaac
     6961 agcgcgcgca cgtccataat cgggctgccg agtttgctgg aaaggtggta ggcaaggacg
     7021 gagccgaggg actcgtggcg caacacggtt tggtgcaaaa aacttgccag catcggttcg
     7081 gcggagaccg cggccgcggt ttcttcgcgg atggtgtgcc agaggtcgaa accggttgtg
     7141 tttaaatggt cttttttcat gagtgatgac gtttgaaaat cgatatggtc ggcagtatct
     7201 taccgtctat attatttttt cggtagggga tttgaaaatg aatttgaaat tctctgcttt
     7261 tgcttgaagt ttcttgaaaa tgtccttatc ttgcgcgggt aataactgga ttttgatttc
     7321 caatttgttt taagggatac gatatgagcg aacagacaca gcagcaaaac agtgaagaag
     7381 cggttgaaaa tgtggaggcg gtggaaaccg tcgagacagt aggaaatgcg gacggtgtgc
     7441 aggaacaggc tgccgcagag ccggcttatg aggatttgca ggcgcggatt gccgagctgg
     7501 aagcgcagtt gaaagacgag cagctgcgcg ctttggcaaa cgagcaaaac ctgcgccgcc
     7561 gccaccagca ggaaattgcg gatacgcaca agttcgccgg acagaagttt gccgtggaaa
     7621 tgctgccggt caaggattat ctggaaatgg cgcttttgga tcagagcggc aatttcgatg
     7681 cgctgaaaat gggcgtgcag atgactttga acgagttgca gaaagcattt gatgctacgc
     7741 aaatcaagga aatcaaccct aaagcgggcg ataagctcga tccgaatatc catcaggcga
     7801 tgcaggcggt ggcaagcgaa caggagccga ataccgtggt gggtgtgatg aagaagggtt
     7861 atacgctgtc cgaccgcgtg ttgcgcccgg ctatggttac ggtggcgcag aaggaagcct
     7921 gaaggcgtct ggggaataat ctgatttatt tcctgaagcg cgttttgcgt ataaaccgat
     7981 cgaagtaaag cggcaatgcc gtctgaaccc gcctgtcggg cttcagacgg cattttatag
     8041 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggaac
     8101 cgattcactt ggtgcttcag taccttagag aatcgttctc tttgagctaa ggcgaggcaa
     8161 cgctgtactg gtttttgtta atccactata tttcggcgtt aacggtcaac ccgtatgccg
     8221 cctgcctgtt tttcttcatc cagtttcttt tgcagggtgt cgcaaggtgt gtcgcagtcg
     8281 cacatttttt tcatacccaa ggcagtaatg ccgccgcaac tgcctttgat gctgcgtttg
     8341 gagaaaatat agccgaccgc cataccgatg atgacggtca ggaagatgcc gaaggtaagg
     8401 agcagggttt tcatggtgtt tcctaatcgg tttgtatgtt tagcggagca gtttttcaaa
     8461 ttcggaagac atggcggtgc ggtagccgcc tttatccctg acaatcagga aaacagcgag
     8521 tttttcgcgc tctgccagct ttaaggcttc ggtttcgccc aatacgaata atcctgtgga
     8581 caagccgtcc gccgtcatcg cactgtctgc gaccacgctg atggaggcga ggttgtggct
     8641 gatgggtcgt ttgttgttcg ggttgatgat atgggagagg cgtttgccgt ttttatcgac
     8701 gtggaaaata cggtaatcgc cggaagtggc aagcgaacgg ttgttcagcg ggacgataat
     8761 ctgcgtattg ccgccttgga cgatattggg ctgctcgata ccgatgcgcc acggttcgcc
     8821 gcgcgcgttt ttgcctttgc cgtgcaactc gccgccgatt tcgaccagat aattttgaat
     8881 gccgtatttt tccagttcgc ccgcaacttt atcaacgccg aagcctttgg caatcgaaga
     8941 taaatccaaa taggccttgg ggtgggtttt gctcaaggaa gcgtaatctt tgccttgttt
     9001 caaaatgatt ttgtctatgc ccgtataaga tgccgcctgt ttgatttgtt ccggcgacgg
     9061 ttcacgggta acggatttgt cggggccgaa tccccaaagg ttgaccaagg ggccgacggt
     9121 tacgtccagc gcgccgtgtg tcaggcggtt caggcggacg gcttcggcag taacgtgtgc
     9181 gaagtcgctt gaaatgcgga ggggcttgcc ggctgtgtgt tggttgaacc ggctgatttc
     9241 ggagtcgggc tgataggtgg acatctgccg gttgacttct ttaagcgcgt catcgatgcg
     9301 tttttgtatt tcggcaggtg aggggagttt gtcccgatta tttgaaaggt atttgacggt
     9361 ataggtcgtg cccatcgttt cgccttgcag ggtaacggtt tgcgcggttt gttccgaaca
     9421 ggcgttcagg aagatgaaac ccagggcaaa tatcaagacg cggataaagt tcggcaggcg
     9481 tgtttcagac ggcatagtgt ttgacggttt tggcaaatgg tttgaattat atcgcaaaac
     9541 ggccggtatg tttctatgcc gatgccgtct gaagggtgtt cggatggcat cggcatagaa
     9601 aaaggaagaa accgaggttt cttccttttg tatttgaagc cgaatattta accgccgaaa
     9661 tcgtccaaga ggatgttttc gtcttccacg cccaagtctt tgagcatttt gatgacggac
     9721 tggttcataa tcggagggcc gcacatataa aattcgcagt cttccggtgc ttcgtggttt
     9781 ttcaggtggt tttcgtaaac cacgttgtga atgaagcccg tgtagccgtc ccagttgtct
     9841 tccggcagcg ggtcggacag ggcgacgtgc cacgtgaagt tcgggaactc tgccgcgagt
     9901 tggtcaaagt cttcgacata gaacatctcg cgtttggaac gtgcgccgta ccagaaggta
     9961 atcttacgtt tggagttcaa acgtttcaac tggtcgaaaa tgtgggaacg catcggagcc
    10021 atacccgcac cgccgccgat aaataccatt tcggcatcgg tgtctttggc gaaaaattcg
    10081 ccgaacgggc cggaaatcgt aactttgtcg ccgggtttga gcgaccagat gtaggacgac
    10141 atttgtcccg gaggcgcatc aggtacgcgc gggggcggcg tggcgatacg cacgttcagc
    10201 ataatgatgc ctttttcttc aggatacgaa gccatagagt aggcacgcaa aatcggctcg
    10261 tccactttgg aaacgtattg ccacaaattg tatttgtccc agtcttcgtg atattcctta
    10321 ggaatgtcga agtctttgta ggcaacagtg tgaggaggag cttcaatttg aatgtagccg
    10381 ccggcgcgga aggggacttc ttcgccttcg ggaatggcaa gcttgagttc tttaatgaac
    10441 gtggctttgt tatcgttgga gatgacggtg cattcccatt ttttcacgcc gaacacttct
    10501 tcgggcactt cgatgtccat gtcggttttg acgctgactt ggcacgacag acggcagcct
    10561 tcgcgtgctt cgcgtttgct gatgtgggac agctcggtcg gcaggatgtc gccgccgccg
    10621 ctttttacga cgacgcggca ttgtccgcac gaaccgcccc cgccgcaggc ggaggggata
    10681 aagatgcctt cgttggcaag cgcgcccaag agtttgccgc cggcgggcat cgtcagctct
    10741 ttttcgccgt tgactttgat ggtgatgtcg ccttcgctga ccagtttgga tttggcaaat
    10801 agaatcatca gtgccaaaac caaaacgatg acggtaaaca tcacgatacc taaaataatc
    10861 tccataccgg tccctttctt ataactggat gccggagaac gacataaacg ccatcgccat
    10921 caggccggcg gcgataaagg taatgcccaa gcctttgagg cctttgggag cgtccgaata
    10981 tttcattttt tcggtaatgc ccgccaaagc gacaatcgcc aacatccagc ccaagcccgc
    11041 gccgaagccg tatacaacgg actcgccgaa gttgtattcg cgttgcgcca taaacgatac
    11101 ggcgccgaaa atcgcgcagt tcacggtaat cagcggcagg tagatgccca acgcgttata
    11161 gagggcgggg acgaatttat ccaagaacat ttccaaaatc tgcaccaaag cggcaatcac
    11221 gccgatgaag gtgatgaatt tcaaaaaggt caaatccacg ccttcgacaa tcgcgccgtc
    11281 tttgagcagc gagtaaacga gttggttgac agggacggac agcccgagta cgaaaattac
    11341 cgccacaccc aaaccgaatg cggtggatac ttttttggat accgccaaaa acgtgcacat
    11401 acccaaaaag aaggatagtg ccatattttc aatgaagacg gatttgatga agaggctcaa
    11461 atagtgttcc atagcttatt cctccgcctg ttcgggtttc caggtacgca gtccccaaat
    11521 caaaaagccg atgatgaaga acgcgctggg ggcgagcagg aacaagccgt tggtctgata
    11581 ccagccgccg tcctgcacgg tttggaaaac ggtgtagccc aagagtttgc ccgagccgat
    11641 cagttcgcgg acggtggcga cgacaagcag catcatcccg tagcccgcgc cgttgccgat
    11701 gccgtcgatc aggctttcca gcggcggctc tttcatcgca aatgcttcgg cgcggcccat
    11761 cacgatacag ttggtaataa tcagaccgac gaatacggaa agctgtttgg acaattcgta
    11821 ggcaaatgcc tgcaagagtt ggtcgaccag cgtaaccagc gacgcgataa tcgccatttg
    11881 cacgataata cggatgctgt tggggatgta gttgcgtacc agcgaaatga agaagctgga
    11941 aaaaccggtt accaaagcta cggaaatacc catcacgatg gccgtctgaa gtttggtggt
    12001 aaccgccaaa gccgaacaaa tacccaaaac ctgcaaggca atcgggttgt tgtcgataaa
    12061 gggtgaaaac atcaaatgtt tcaagcgttt catatcagcc attattgcgc tcctgctgat
    12121 ttcaatttgt tcaggtaggg gatatagccg ttttcgccga accagtaggc gaacgaacct
    12181 tgcacgcctt tggatgtcag cgatgcgccg gagagggcat ctacgccgtg ttctttgtcc
    12241 gaacccgcgc ctttgccgac gtgcagggcg agtttgcctt gtccgtcaaa cagttttttg
    12301 ccgacgaatt tttgctgcca caacggattg ccgatttcgc cgcccaagcc cggggtttcg
    12361 ccttgttcgt agtaggtaat gccgttgatg gtgttgccgt cgggctggat ggcgacaaag
    12421 ccgtacatga ccgaccacaa accgttaccg tgcataggca ggatgatttg cccgattttg
    12481 ccgtcttcgc cttttaccaa ataaacctcg gtgtatttgg cacggctttt gatgcctgcc
    12541 aaatcgtctt cgggtttgat gcggatgctt tgggcagggt ctttgcctgc gatgcgcgcg
    12601 ctgaagtctt taggcgcatc ggcgacgtat tcgccggtcg ccaaatcgac aacacgttgc
    12661 tcgatacgct cggcaaaggt tttaccgatg tcggtgtcct tatccatcaa accggctacg
    12721 ctcaagatat agccttgttt gtcttggagt ttttgtttct cttggatggg tttcaagccg
    12781 acgaccgcac cggcaacgat gaccgagcaa atcaggctga ccgccaacac gacaatcagc
    12841 gtaccgctga agctgtcttt atcgaatttc ttagccattg ctgcgcgcct ttctgcgttt
    12901 gatgttcgct tgtgcgacga aatagtcgaa aatcggggca aacaggttgg caaacagaat
    12961 cgccaacatc atgccttcgg ggtaagccgg attgaccacg cggattaata cgcacatcac
    13021 accgatcagt gcgccgtacc accatttgcc gacattggta aaggaagcgg aaacggggtc
    13081 ggtcgccata aacagcatac cgatggcgaa gccgccgacg accaaatgcc agtaccaagg
    13141 catagcaaac atagcgttgg tgtccgaacc gatgaagttg aacagcgaag acatcgcaat
    13201 cataccgatc atcacgccgg caataatgcg ccaagaagcg atgcgggcaa acacgataaa
    13261 cgcgccgccg attaagagtg ccaaagtgga gacttcgccg atggagccgg gcagtttgcc
    13321 gataaacgcg tccatccaag tgatggtttg accggttacg gcgtttttca ggccgtctgc
    13381 accgtgtgcc gcccattgcg ccagcgcggt tgcgccggaa tagccgtcaa ccgccgtcca
    13441 aaccgcatcg ccgctcaagt tggcggggta ggcgaagaac aggaaggcgc ggcctgccag
    13501 cgcagggttc ataaagtttt tacccgtacc gccgaatact tctttcgcaa ccacaacgcc
    13561 gaaagaaata cccaaagccg cctgccacag cggcagcgtg ggcggaacga ttaaggcaaa
    13621 cagaatcgag gtaacaaaga aaccttcgtt gatttcgtgt ttgcgcacgg tggcgaacaa
    13681 gacttcccag aaaccgccga cggcaaatac ggtcgcgtaa atcggcagga agtaaatcgc
    13741 gccgaacagc attttgccca acacgctcgc ttcagacgac atattgatgc ccaaagcgtc
    13801 ggcaagggtg taatgccagt cgttggcgat gttttgttgc agcaaatcag gcgttaacgc
    13861 accgaatgcc tgtacgccga cgttgtacat accgtagaac atggcaggga acaaagccag
    13921 ccacaccaaa atcatcatgc gtttggagtc gagcgcatcg cggacgtgtg ccgctttgcg
    13981 cgttaccgca ccggatgtat agaaaatcgt cgccgcagct tcgtagaggg cataccattt
    14041 ttcatgtttg ccgcctggca ggaagtgcgg ttcgattttt tccagaaaat gtttcaagcc
    14101 cataatcagc cttccttctc aatggtttcc agcaccttac gcaacagcgg gccgtattcg
    14161 tatttgcccg ggcagacgaa gctgcacaaa gcgaggtctt cttcgtccaa ttccaagcaa
    14221 cccaatgcct gcgcgctgtc ggtatcgccg acgattaaat cgcgcaaaag cagggtaggc
    14281 aggatgtcta gcggcattac gcgctcgtaa gtaccaatcg gcaccatggc gcggtcgcca
    14341 ccgttgacgg ctgtcgtgaa cttgaagagt ttgtttttca ggaaatgacc gagggtcgta
    14401 cgcgtgatcg agtatttgtc cggttgtggc gcaacccagc cgaacagttc tttactgcgg
    14461 ccttcttcga taacggaaat ctgattgtgg tagcgtccca aataatcgtg cgcgccttgt
    14521 gcaatcgcgc cgttcaatac cgaaccggaa atcacgcggt tgtctgcgtc aaccaattcg
    14581 cccgcagtaa tttgcgatac tttcgcaccc aaaacggtac gcaagaggcg tggtttgttg
    14641 acttgagaac cacccaaggc aaccacgcgc tcggtgttca gacggcctgt tgcaaacaaa
    14701 cgtccgatgg caattacatc ttgataattg atggtccaaa cggttttgtt cgcaccgaca
    14761 ggctcaatga aatgaatgtg cgtgccactc aaaccggcag gatgcgggcc gccaaattca
    14821 tgagtttcga tgttggcggc gttttcagac ggcacgtctg cgcctgctgc tttacacaca
    14881 tggattttac gctcggtcaa acggctcaat accagcaaac ctcgtctgaa atcctcggcg
    14941 gcttctttga tcacaaccac agggtctgcc gccagcggat tggtgtccat cgcattgacg
    15001 aagatggcga acggcgcggc atcgacggca gggattttgc tgaacggacg ggtacgcagc
    15061 gcagtccaca aaccggattg gatcagattg cggcgcactt cttcgccgct taagtttgcc
    15121 aacgcttcgg gtgcgtagcg ttcaaactcg atttcgtcgt tgccttcaac ggcaatcacg
    15181 actgactgaa gtacgcgctt ttcgccgcga tggatggcgg cgattttgcc agaagccggc
    15241 gcggtaaaca ccacgcccgg attctttttg tcttcaaaca gcacttggcc ttttttgacg
    15301 gcatcgcctt ccttgacttt catcgagggg cgcataccgg catattcttc gccaagcaac
    15361 gcgacttcgg taatggccgg gccgtcataa atgacttgct ccggtctgcc cgcgatgggc
    15421 aggtttagac cttttttgat tttaatcata tatttgcatt acttgtgatg gttaaggtaa
    15481 aaacggcgtg ttttgatacc gtgtcgcgtg gcatcaaaag cattgaataa attaatgtag
    15541 caaagtgtta gattctatca ggaattgtac ctgtttgtca gatttgctgc ttttttcctt
    15601 gcggaagccg tttttatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc
    15661 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    15721 attgtcggaa ggggggatat tgatttgatt atgccggaat ttaaaatgcc gtctgaatgt
    15781 tcagacggca tagcgtttac agcagtttga aaacgaaaaa gataagggta tgtacgatga
    15841 agacgggtgt caggaaggcg accgaccaca tcatatagcc gaagaaagtc ggcatcggta
    15901 cgccgcgctg ttcggcaatg gccttgacca tgaagttcgg tgcgttgccg atgtaggtca
    15961 gtgcgcccat gaataccgaa cccatagaaa ccgccagcag cgaatgaaac agggtacccg
    16021 tcatcaaggc ttgggcatcg ccgcccgcca tattgaaaaa aacgagataa gtgggcgcgt
    16081 tatccaagaa tgccgacaat atgccgctca tccaaaaata catcacatta atcggatgac
    16141 ctgccgtatc gtgaaccagc gataccaccc cgcccagcgc gcctgcctcg cctgctttca
    16201 gaatgctcag gacgggaaag atggtgatga agatgccgag gaagagtttg cccacttcgg
    16261 cgatgggttc aaagttgaat tcgttgcctg cgcggacttg tttgggcgtg attgccatag
    16321 atacggcggt caatgcaatc aggatgacat cgcggacgag gttttgcagg gcgtaacggc
    16381 tgccgaggat ttcaaatccc gggtgttcgg gtttccaaag gccggacatt agaaccgcgc
    16441 cgaccacgcc cgaaagcagg aggaagttcc atttgccgaa gatggcgatt ttttcgggtt
    16501 tttcctgttg tgccggcgta tcttgtgcaa tgctttcctg tttgaagaaa cggttgtcga
    16561 tgaaatagaa ggcggtcaac aggacagcgg tgctgatcag gacgggggcg aacatatgtt
    16621 tgaccgtcca catgaaatct acgcctttga ggaagccgag gaagagtggg gggtcgccca
    16681 aaggggtcag accgccgccg atgtttgcaa ccaagaaaat gaagaagatg acgatgtgca
    16741 cgcggcgggt acggttttgg ttggctttca gcagcggacg aatcatcagc attgctgcgc
    16801 cggtcgttcc catgatagag gcaagtgcag taccgacggc aagcagggcg gtgttgagct
    16861 tgggtgtgcc gttcaagtcg ccccaaacca aaatgccgcc tgaaatggtg tacagggcaa
    16921 gcagcagcag gatgaagggg atgtattctt caacgagtgc gtgtgcgacg gtatggatac
    16981 cggcggacgc gccaaaaacc aaactgaacg ggatgaggaa gagcaatgtc caaaaggcgg
    17041 taattttgcc gtaatggtga tgccaggtat gcgaaaaaaa caagggaccc aatgcgatag
    17101 acagcaaaat cagggcaaag ggcaggcccc acagcaggtt taggtttgcg ccgtccaaat
    17161 ctgcggcgta aaccgatgct gggaaaagca ttagtgaaaa caggggtagg tggcgcatcg
    17221 tgtttcctcg attcaagcac tgccttgcgc ggcgcgtggg agtgatacag gcaccgtgcc
    17281 gcccggacat aggcggactg tgctgcctgt ttgtttttga aaaaagtttt ccgattgatt
    17341 gtaaagtaaa taatcaggat aaaccagttt cccaaaccgg aaggcggcgg gaaggcggat
    17401 tgccgtgctt gggaatatat gttctttttg ataaataatt ttatttaaaa caaataaata
    17461 tatgtcttta ataaataatc tatcgaaaac gaaaaatgaa tttattttaa catatatttg
    17521 caatgaaaca ggtttgcccc ccgtttgttt gcccttatcc ctttcagtac ggcattcaag
    17581 attcgggcct gcgccacatc catatggcga caagggaaca aaaaaccgat gaaaccgccc
    17641 cgacccacca gcgttgggga aactgccaaa acattatcag gcaggatgcg gtcatcatac
    17701 tgatggcgaa tattttggct ttgcgcggca ctgcgccgtt ttgttcccag ttatgaacca
    17761 tcggaccgaa atagcggtgc cggtgcagcc agcggtgaaa gcgcggggat gcctttgccc
    17821 agcaggcggc ggagagcagt acgaacggcg tggtcggcaa cagcggcaaa aaaatgccga
    17881 tgatacccaa cagcagggaa atgcagccgc aggcaattaa aagataacgt atcattttga
    17941 aatatttttc ttattgtgcg gataagggca ggatgtgata ccgagttttg cccagccttc
    18001 atgtcccatt ttttccagca gggcgatatt gcgttcgaat attgccgaag cgtcgggaaa
    18061 ggcttgtgcg gctttggcaa tgctgtcttc gcggatcaga tgcagtgtcg gatagggaga
    18121 acggttggtg tagttgccga tgtcgtcggg ttcggtattc tgaaagcgga attggggatg
    18181 gaacggtgcg atttggagga tgccttccaa gccgttttcg acaacggcgg catcggcaat
    18241 atcgagcata tcgttgaata cgtcgaaatc ggggaatagg gtcgggtgaa ccagcagggt
    18301 ggtttccagt tcggtggcgg gtgtattgcc cagtcgctgc agttcttcgt ccaagtcttc
    18361 caaaaaaccg tcaaggtgtt tggcttcgct gatcgcgatg cggacaaggt ttttaacgtg
    18421 gggggctttg gcaaagggac acaggttcag accgatgacg gctttttcca accattgtcc
    18481 ggtgtgttcg gcaacagcat ctttattttc ggaagtattg atattcatta ttgtcatgta
    18541 aatgtgtttg cagattgcac gtgcgggaaa atcgggaagg gcactattcc ttcagcaggt
    18601 ggttgagtgg cagggaggtg gtgtgtttga tttcttttaa aacaaagctc gattgggcat
    18661 cctgtacgcc gtggtgggac aggagcgtat ccaaaacaaa atgggaaaac gcgttcatat
    18721 cggtaaaaaa cgcctgaagc aggtagtcgg tttcccctgt cagggcgaag cagctcaaga
    18781 cttcaggcca ttttcgaacc gatgcggcaa agtcttcccg cgcgtctttt gctttgcgga
    18841 tggaaacgcg gataaatgcc tgaagcccca ggttgacgga ttccggagac agcagcgcgg
    18901 catattggcg gacgatgccg gcatcttcca actgcttcag acggcgcagg cacggagaag
    18961 gcgaaagtgc gacacgttcg gacagttcga cattggtcag cctgccgttt tcctggagaa
    19021 cctgtaagat tttaatatcg gttttgtcta aagtgagttg gggcatattt gcgttccgtt
    19081 ttaaggaatt cggattgtct gtccgtatgt ttgcggcaat ccgcacagat ggagaccata
    19141 ttaacatata aaaagttata ccgtcatccg ggacaaattt tgttttcgga aaatcatgtg
    19201 aaaacagagg cggtcggttt gcatctcttt taagacggct tgcccaaacc gccgattcaa
    19261 gacataatcg ggaaatgtgc aggagagtgt tacacccaac tacaatgtaa ccaccgaagg
    19321 cgcagacacc cttaaatcgc tcaggtatca gggactgcac attgaaacaa acaatctgga
    19381 gagcggcgtt ggaataacgt ccaccgaagg ggagaaggcc gtctgaacca ccattcagac
    19441 aaccgcgcaa agcagtgagc agactggttt gccatcatgc ggatacggcc gaaaatctca
    19501 ggttcaagga cagatagggt catccgcgca caggtgcgcg ggtggcatct gaacaaaaaa
    19561 tccggagaaa cttgagaatg attactctga aaaccacccc atttcatcaa gcccatcaag
    19621 atgcaggcgc gaagctggta gattttgccg gctgggagct gcccatccat tatggttcgc
    19681 aaatcgccga acacgaagcc gtgcgcaccg acgcaggtat gtttgacgta tcccatatgc
    19741 ttgttaccga cgtagcaggc gcaaatgcca aagccttttt ccgcaaattg attgccaacg
    19801 atgtcgccaa gctcgctttt gtcggcaaag ccctttattc cgctttgctc aacgacaacg
    19861 gcggcgtgat tgacgacttg atcgtttacc gcaccaatga agccgaaacc caatactgca
    19921 tcgtgtccaa cggcgcgact cgcgaaaaag atacggcaca attccacaaa gtcggacaag
    19981 agttcggcgt ttctttcaat ccgcgctacg acctcggcat gctcgccgta caaggcccga
    20041 aagccatcga gaaactcctg accgtcaaac ccgaatgggc agatgtcgtc aacaacctca
    20101 aaccgttcca aggcgcggat ttgggcaacg actggtttgt cgcccgcacc ggctacaccg
    20161 gcgaagacgg cgtcgaagtc atcctgcccg gcaccgaagc cgtcgcattc ttcaaagccc
    20221 tgcaacaagc cggcgtacaa ccctgcggcc tcggcgcgcg cgataccctg cgcatggaag
    20281 ccggtatgaa cctctacggc aacgatatgg acgacgacac cagcccgctc gaagcaggca
    20341 tgggttggac ggttgatttg aaagacgaaa gccgcgattt tgtcggtaaa gccgccttgc
    20401 tggcattgaa agaaaaaggc gttgccgtca aacaggtcgg tctgttgctc gacaaaggcg
    20461 gcatcctgcg cgcgcatatg gaagtgttga ccgacaaagg caaaggcgaa accaccagcg
    20521 gcgtattctc tccaagtctg aaacaatcca tcgccatcgc ccgcgtgccg aaagactttg
    20581 acggcgatac cgccaaagtt ctgattcgcg gcaaagaagt ggacgtacgc gtactgaagc
    20641 tgccgtttgt gcgcaacgga cagaaacagt ttgattgatg cggtttcaga cggcattttc
    20701 atttcatatg ccgtctgaaa gcaggtttta attgttgtcc gatacggacg tttgtagaaa
    20761 gcattgaaca aggcatctgt ggatattgat tcatgcagat gccgtctgaa aataacccct
    20821 atcaatgagt atcaaaccat gagcaacaac atcccggccg aactgaaata cgttgccagc
    20881 catgaatggc tgcgccttga agaagacggt acgattaccg tcggcattac ccaccacgcg
    20941 caagagctgt tgggcgacat cgtgttcgtc gagctgcccg aagtcggcgc gaaccttgcc
    21001 gctgaagagc aagccggtgt ggttgagtct gtaaaagccg cgtccgacgt gtacgcaccg
    21061 attgcaggcg aagtcgttgc cgtcaacgaa gatttgccaa gtgctccgga aactgccaac
    21121 agcgattctt acggcgcagg ctggttcttc aaaatcaaac ctgtcaatcc tgccgattac
    21181 gacggtctgc tgactgccga acaatacgcg ggcgaagtgg actaaatctc tatcctgata
    21241 atgggatgac tgatttgaga aactgcactt tcagacggca aggatgcgtt tggaagtgca
    21301 gtttttgtct gtgtcttcaa agttcagtta ggctataata acggtctata ttcatcttta
    21361 ccgatttttt catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca
    21421 tacgggaaaa gctggcgttt gccgccgccg ccctgcctaa agccgtccgc aatcttgccc
    21481 gaagcaatgc ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact
    21541 gcgtcggtga ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg
    21601 aagaaatccg tccgtatctg tacgcgctgg atatgcagga aaccgtgcgc catgccttcc
    21661 gcgttgcctg cggcttggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta
    21721 aggatgcggt cagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt
    21781 tccaaaaaac cttttctgtt gctaaagagg tccgtaccga tactgccgtc ggcgaaaact
    21841 cggtttccat ggcttccgct tccgtcaaat tggcggaaca gatttttccc gacatcggcg
    21901 atttgaatgt cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg
    21961 ccgccaaaag tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt
    22021 tgtgcgacaa gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgccattc
    22081 tgcacgatta cgacgtagtg gtttcttcaa cggcaagcca gttgcccatt gtcggcaaag
    22141 gcatggtgga gcgtgcattg aaacaaaggc agagtatgcc gttgttcatg cttgatttgg
    22201 cagtgccgcg tgacattgaa gcggaagtcg gcgatttgaa tgatgcctat ctttatacgg
    22261 tggacgatat ggtcaacatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg
    22321 ccgccgaaac gctggtatcc gagaaggttg ccgaatttgt caggcagcag cagggcaggc
    22381 agagcgtccc cttgattaaa gccttgcggg acgagggcga gaaagcgcgc aagcaggtgt
    22441 tggaaaatgc gatgaaacag cttgccaaag gcgcaacggc agaagaggtt ttggaacggc
    22501 tgtccgtcca actgaccaac aagctgctgc attcgccgac ccaaactttg aataaggcgg
    22561 gggaagaaga taaagatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac
    22621 ggtgcgccgg aaaaggatgc cgtctgaagg ggtttcagac ggcatatttt tgtgctgtct
    22681 gacaacatcg tgaaatccca cattatatcg atgtaatcac aaagtatagt ggattaacaa
    22741 aaatcaggac aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac
    22801 gctgtactgg tttaaattta atccactata ttatcccgta tgcggattgg ttttaagatt
    22861 tgtaaatttg atttgcatca aaaaatcgcc gatagatgat tcatataata tcaatattaa
    22921 agagtatcgg tatatcgggg atagtcatgt cctgtttttc aatcaaacgt atgtccgcgt
    22981 ttcgggcgcg gataacggcg ttttttgccg cctttgtctt tttgacggcg gcactgcccg
    23041 cttatgcgga gcgtctgcct gattttctgg cgaaaataca gccttcggaa atttttccgg
    23101 gtgcggaccg ttacggcaag ccggaaggta agcctatggt tgcccgcgtt tacaaaggcg
    23161 atgagcagct cggtttggtc tatatcacaa ccgatgtggt caatacgcgc ggttattcga
    23221 gcaaaccgat tgatacgctg atggcgttgg ctaaagacgg tacgatagcc ggagcgaaat
    23281 tggttgatca ccatgagtcg attatgctga tcggtatccc gcatttgccc gcgcccgggc
    23341 gggcgatacg ctcaaactgg cttccggcgt atatcaaacc aaacttcaca tcgacaaacc
    23401 gattacgatt gaagggcctg ccgaccgttc tgcaaccatc gaaggagaca ggagcgggcg
    23461 taccatagcc gtacacgcgc cggacgtaac gctccgcaac ctgaccgtta cccgttccgg
    23521 tatgagcctg cccgcaatgg atgccggtat ttatctcgaa gaaactgccc cgcgcgccct
    23581 gattgaacac aataatattt tggataattc ggtcggcgtc tatctgcatg gttctgccga
    23641 tgcgatggtg cgggagaata aaatcgtcgg cgacgcgact ttgcgcgtga acgagcgcgg
    23701 caatggcgtt accgtttgga acgcgcccgg cgcgcaggtc gtcggcaacg atatttccaa
    23761 agggcgggac ggcatttttt ccaataccag cacgcacaac acctataaaa acaaccgctt
    23821 cagcgatttg cgtttcgccg tccactatat gtacaccaac gacagcgaaa tcagcggcaa
    23881 tatttccgtg ggcaacaata tgggctatgt gctgatgttt tccgagcggc tcaaagtgtt
    23941 tgacaatatc gccgtcggca gccgcgacca aggcatcatg ctcaactatg tcaactattc
    24001 cgatattcac gacaacatta tcaacaaagc gggcaagtgc gtttttgcct acaatgccaa
    24061 ctacgataaa ctgtccgcca atcattttga aaactgccaa atcggcatac actttaccgc
    24121 cgccatcgaa ggcacgtcac tgcacgacaa ttcctttatc aacaacgaaa gccaggtcaa
    24181 atacgtcagc acgcgctttc tcgactggag cgagggcgga cacggcaact attggagcga
    24241 caacagcgcg ttcgatttga acggcgacgg cttcggagac agcgcgtacc gtcccaacgg
    24301 catcatcgac caaatcatct ggcgcgcacc cgtatcgcgc ctcttgatga acagtcccgc
    24361 aatcagcatc gtcaaatggg cgcaggcgca gtttcccgcc gttctgcctg gcggcgtggt
    24421 ggacagcaaa ccgctgatga agccttatgc ccccaaaatt caaacccgtt atcaggcgat
    24481 gaaggacggg ctgctcaaag aagccgaaac gcggcagttg gaatggggca gggcggaaaa
    24541 cggttctttg aactagtctg tttcagacgg catccggatt caaatgccgt ctgaaaacac
    24601 aaaaggaaaa accatgacca cacatcatgt cgaattgagg aatgtaacca aacggttcgg
    24661 ggcgcaaaaa gcggtcaacc aagttgattt ggttttgaag gcaggagaaa gcgtcgggct
    24721 tgccggacac aacggcgcgg gcaagtccac cattatgaag ctgatactcg ggctgattac
    24781 cccgaccgaa ggcgaagtga tgcttttggg cgaacgtacc ggtagcaaag cgggggcgcg
    24841 gcttcgcagc caaatcggct acctgcccga aaccgttgcg ctgccccctt cgctgaccgg
    24901 catcgaaacg ctggattttt atgccaaact taaaaaacag ccgctcacgc agaaccgggg
    24961 gctgcttgag cgtgtcggta tttcacaggc ggcacaccgc cgcgtcggca cttattctaa
    25021 agggatgcgc caacgccttg ccttggcaca agccctgctg ggcgagccca aagtcctgct
    25081 gtttgacgaa ccgacaaccg gcctcgaccc tgcatcacga caaatgtttt acgaagtcgt
    25141 gcgcgaactc aacgggcgcg gcgcgaccgt attgctcagc acccacgccc ttgccgagtt
    25201 ggacggacac gccgaccgca ttatcgtgga ttaaatttaa accagtacgg cgttacctcg
    25261 ccttgccgta ctatttgtac tgtctgcggc ttcgttgcct tgtcctgatt tttgttaatc
    25321 cactatatgc gggtatggcg ggtttgagcg gacaaatcag cctgaccgtc cccgttttgc
    25381 tgaccgctca ggttttatgg gttatcattc cgcttgtttt ggcagccgga atttttagaa
    25441 agcgacaaat atgaaaaaaa ccctgttggc aattgttgcc gtttccgcct taagtgcctg
    25501 ccggcaggcg gaagagggac cgccgccttt accccggcag attagcgacc gttcggtcgg
    25561 acactattgc agtatgaacc tgaccgaaca caacggcccc aaagcccaga ttttcttgaa
    25621 cggcaaaccc gatcagcccg tttggttctc caccatcaag cagatgttcg gctataccaa
    25681 gctgcccgaa gagcctaaag gcatccgcgt gatttacgtt accgatatgg gcaatgttac
    25741 cgattggacg aatcccaatg ccgacacgga gtggatggat gcgaaaaaag ccttttacgt
    25801 catcgacagc ggctttatcg gcggtatggg tgcggaagac gcgctgccgt tcggcaacaa
    25861 ggaacaggct gaaaaatttg caaaggataa aggcggtaag gttgtcggtt tcgacgatat
    25921 gcctgatacc tatattttca aataatattg aaaaaaccgg ccgaaacttt aaaaacgagt
    25981 ttcggtcggt ttttcttttc ttgttcggta tagtgtcggc aggaaagaac cttcacatcc
    26041 cgccgtaatt cggcccgctc gcgccttcgg ggcaaatcca agtgatgttt tgcgtcgggt
    26101 ctttgatgtc gcaggttttg cagtgcacgc agtttgccgc gttgatttgc aggcgcggat
    26161 tgccgttttc ttcaacaatt tcgtacacgc cggccggaca atagcgcgtt tcgggcgagg
    26221 cgtattcttt gtagttcacg tctatcatcg tttgcggatt gttcagcacc aaatggtcgg
    26281 gctggttttc ttcgtgcgcg agattggcaa ggaagacgct gctcaagcgg tcgaaggtca
    26341 acacgccgtc gggtttcgga taatcaatcg gtttgcacgc ggcggctttt ttgagctgct
    26401 cgttgtcttt gccgtaatgt ttcaaggtcc acggggcttt gcctctgaaa atcatctggt
    26461 cgatgccggt atagattgag ccgaggtaaa cgccccattt gaatgacgga cggacattgc
    26521 gcgcggcgta aagctctcga tacagccagc tttgttcaaa acgttgctga taatccgccg
    26581 cttctttgcc gctgtcgaaa ccctccgctt cttcaaggtg ttccaacaag gggaacacgg
    26641 cttcggcggc gatcatagcg gatttcatcg cggtatggat gcctttgatg cgcggcatat
    26701 tgaggaaacc tgccgcatcg ccgattaaaa cgccgccttt gaacgagagc ttcggcaaac
    26761 tttgcaaacc gccttcaatc agcgaacgcg cgccgtaagc aatgcggcgg ccgccttcaa
    26821 aggttttgcg gatttcggga tgggttttga aacgttggaa ctcttcaaac ggcgacagat
    26881 aaggattttg atagtccaaa ccgaccacga agccgacggc gactttgttg tcgtcgaaat
    26941 ggtaaacaaa cgcgccgccg taggttttgc tgtccagcgg ccagcctgcg ctgtgcacca
    27001 ccaaaccggg ctgatgctgt tcggacggca cttcccaaac ttctttcatg cccaagccgt
    27061 aagtttgcgg ctggctgttt tggtcgagtt tgaagcgttc gatgatttgt ttggaaagcg
    27121 aacctcggca gccctcggca aacagggttt gccgtgccca aagctccatg ccgggctgga
    27181 atgaatcggt cggctcgccg tctttgccca cgcccatatt gccggtcgca atgcctttga
    27241 ccgaaccgtc ttcgtgatac agtacttcgg cagcggcaaa gccaggatag atttccacgc
    27301 ccatattttc cgcctgctcc gccaaccagc gcacgacttc gcccaagctg acgatgtagt
    27361 tgccgtgatt gtcgaaattc ggggtaatag gcagattgaa cgcctttttc ttagtcagga
    27421 acaatacttt gtcctgcgtt accgtacgcg tcagcggcgc gcctttttct ttccaatcgg
    27481 gaatcagctc gttcagcgca atcggatcga taaccgcgcc cgccagcgaa tgcgcgcccg
    27541 tctcagaacc tttctccacc acgcaaacgc tgatttcgcg cccgttttgt tcggcaagct
    27601 gcttgagttt gatggcggca gacaaacccg acgggcctgc gccgacaatc acgacatcgt
    27661 attgcatact gtcgcgggtg atggattctg tcatggcggt tcctgtgtat ttattattga
    27721 attgcaaatc cgtaattata caacgggaac atatagttac caaatacaac aaaggtcgtc
    27781 tgaaaaccat attttcggtt ttcagacgac ctttgtcgaa atttcaataa gcacgccacc
    27841 attttacctg tccgaccgca aactccgtct gacgtttcgg actgcgtgtg aaaaacgcct
    27901 tatccccgcc ggcatccctc cctttcggca c
//
LOCUS       DAXIHS010000020        27120 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXIHS010000020 DAXIHS010000000
VERSION     DAXIHS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.020"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(320..2296)
                     /locus_tag="ACFOF2_000731"
     CDS             complement(320..2296)
                     /locus_tag="ACFOF2_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC7107085.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIESKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNGLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRHRDGLLEALQD"
     gene            complement(2307..2867)
                     /locus_tag="ACFOF2_000732"
     CDS             complement(2307..2867)
                     /locus_tag="ACFOF2_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC7107086.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2864..3918)
                     /locus_tag="ACFOF2_000733"
                     /pseudo
     CDS             complement(2864..3918)
                     /locus_tag="ACFOF2_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(3932..4279)
                     /locus_tag="ACFOF2_000734"
     CDS             complement(3932..4279)
                     /locus_tag="ACFOF2_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC7107087.1"
                     /translation="MDKELNPDTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(4379..5047)
                     /locus_tag="ACFOF2_000735"
     CDS             complement(4379..5047)
                     /locus_tag="ACFOF2_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC7107088.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(5044..6189)
                     /locus_tag="ACFOF2_000736"
     CDS             complement(5044..6189)
                     /locus_tag="ACFOF2_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFC7107089.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(6179..7510)
                     /locus_tag="ACFOF2_000737"
     CDS             complement(6179..7510)
                     /locus_tag="ACFOF2_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFC7107090.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7510..9681)
                     /locus_tag="ACFOF2_000738"
     CDS             complement(7510..9681)
                     /locus_tag="ACFOF2_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HFC7107091.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9836..10360
                     /locus_tag="ACFOF2_000739"
     CDS             9836..10360
                     /locus_tag="ACFOF2_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107092.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLRQRIQSDR
                     SNSAKRRSGRWYFI"
     gene            complement(10462..10731)
                     /locus_tag="ACFOF2_000740"
     CDS             complement(10462..10731)
                     /locus_tag="ACFOF2_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107093.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10671..11036)
                     /locus_tag="ACFOF2_000741"
     CDS             complement(10671..11036)
                     /locus_tag="ACFOF2_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107094.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(11040..11417)
                     /locus_tag="ACFOF2_000742"
     CDS             complement(11040..11417)
                     /locus_tag="ACFOF2_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC7107095.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11482..12891)
                     /locus_tag="ACFOF2_000743"
     CDS             complement(11482..12891)
                     /locus_tag="ACFOF2_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFC7107096.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGVAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12894..13091)
                     /locus_tag="ACFOF2_000744"
     CDS             complement(12894..13091)
                     /locus_tag="ACFOF2_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFC7107097.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(13088..13738)
                     /locus_tag="ACFOF2_000745"
     CDS             complement(13088..13738)
                     /locus_tag="ACFOF2_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp37"
                     /protein_id="HFC7107098.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13728..14150)
                     /locus_tag="ACFOF2_000746"
     CDS             complement(13728..14150)
                     /locus_tag="ACFOF2_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HFC7107099.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(14150..14548)
                     /locus_tag="ACFOF2_000747"
     CDS             complement(14150..14548)
                     /locus_tag="ACFOF2_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107100.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(14613..15521)
                     /locus_tag="ACFOF2_000748"
     CDS             complement(14613..15521)
                     /locus_tag="ACFOF2_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFC7107101.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(15521..16582)
                     /locus_tag="ACFOF2_000749"
     CDS             complement(15521..16582)
                     /locus_tag="ACFOF2_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC7107102.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKSETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(16813..17229)
                     /locus_tag="ACFOF2_000750"
     CDS             complement(16813..17229)
                     /locus_tag="ACFOF2_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC7107103.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(17357..18682)
                     /locus_tag="ACFOF2_000751"
     CDS             complement(17357..18682)
                     /locus_tag="ACFOF2_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC7107104.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(18669..20237)
                     /locus_tag="ACFOF2_000752"
     CDS             complement(18669..20237)
                     /locus_tag="ACFOF2_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC7107105.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(20237..21859)
                     /gene="terL"
                     /locus_tag="ACFOF2_000753"
     CDS             complement(20237..21859)
                     /gene="terL"
                     /locus_tag="ACFOF2_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC7107106.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDALPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIATANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            21871..22383
                     /locus_tag="ACFOF2_000754"
     CDS             21871..22383
                     /locus_tag="ACFOF2_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107107.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            complement(22380..22886)
                     /locus_tag="ACFOF2_000755"
     CDS             complement(22380..22886)
                     /locus_tag="ACFOF2_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC7107108.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(22889..23086)
                     /locus_tag="ACFOF2_000756"
     CDS             complement(22889..23086)
                     /locus_tag="ACFOF2_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107109.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(23083..23349)
                     /locus_tag="ACFOF2_000757"
     CDS             complement(23083..23349)
                     /locus_tag="ACFOF2_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107110.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(23361..23699)
                     /locus_tag="ACFOF2_000758"
     CDS             complement(23361..23699)
                     /locus_tag="ACFOF2_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107111.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(23696..23914)
                     /locus_tag="ACFOF2_000759"
     CDS             complement(23696..23914)
                     /locus_tag="ACFOF2_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107112.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            23927..24097
                     /locus_tag="ACFOF2_000760"
     CDS             23927..24097
                     /locus_tag="ACFOF2_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC7107113.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(24142..24447)
                     /locus_tag="ACFOF2_000761"
     CDS             complement(24142..24447)
                     /locus_tag="ACFOF2_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107114.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(24344..24763)
                     /locus_tag="ACFOF2_000762"
     CDS             complement(24344..24763)
                     /locus_tag="ACFOF2_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107115.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQVVIE
                     KMKRDKAQAMLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            complement(24735..24971)
                     /locus_tag="ACFOF2_000763"
     CDS             complement(24735..24971)
                     /locus_tag="ACFOF2_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107116.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETASE"
     gene            complement(24974..25138)
                     /locus_tag="ACFOF2_000764"
     CDS             complement(24974..25138)
                     /locus_tag="ACFOF2_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107117.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(25141..25347)
                     /locus_tag="ACFOF2_000765"
     CDS             complement(25141..25347)
                     /locus_tag="ACFOF2_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107118.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(25344..25886)
                     /locus_tag="ACFOF2_000766"
     CDS             complement(25344..25886)
                     /locus_tag="ACFOF2_000766"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC7107119.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
     gene            complement(26194..26622)
                     /locus_tag="ACFOF2_000767"
     CDS             complement(26194..26622)
                     /locus_tag="ACFOF2_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC7107120.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(26603..27037)
                     /locus_tag="ACFOF2_000768"
     CDS             complement(26603..27037)
                     /locus_tag="ACFOF2_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC7107121.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT         5605 a         7680 c         6967 g         6868 t
ORIGIN      
        1 ctttcgcggg aatgacggcg gcgggggttt ctgttttttc cgatagattc ctgccgcgtt
       61 tggggtctag attcccgctt tcgcgggaat gacggtggcg ggggtttctg ttttttccga
      121 tagattcctg ccgcgtttag gggctggatt cccgctttcg cgggaatgac gaaagcggcg
      181 ggaatgacga agagtggcag gattggcggg gcataggttt cgggggattg gcggcgcggc
      241 cggtttcggt cggattgctt ggatcggcgg gctgaagccc atctctacag ccatctctac
      301 aacccatctc tacagcccgc tagtcttgca gtgcctcgag caggccgtct cggtggcggc
      361 ggcggtttcg gggtccggtt gatgttcttc cactctctct ttgcggttct tcgttggttt
      421 caggtatttc ggggcctatc ggggagctgg cggcgtttcc cgaggcggct ttgccgatgg
      481 ccatccagtc gcaactgcct tcgtaccagt tggtaataat ggcggcggcg tggttttttg
      541 cgcggataaa cagccagttg tgttgccttc gatcgccggt gacgcgtccc gaatggcgtt
      601 cggatacgaa gcatttgacg ttgccgtcgg caaaggcgac ggggaagacg acttcgtttt
      661 cgatggggcc gccgcttctt gtaaacctgt aactgccggt ctgtatcatt gtgccgtcgg
      721 ggaatctgac aatatccacg ccgccgatgg tttggcgtgt gaattgcgcg gcaacggcgg
      781 tgctgatttt ttcgtccagc ccttggattt gggcggcggt gtgggtgtgg gcgcggtcgg
      841 ctttgtcttg caggttttgc gccagcgtgc ccgcgtccag cgcgccggcg ttgatggttt
      901 cgccgtggga ctttatccaa aacacggctt cgcccaaggt gtcggcggct ttgatgcaca
      961 gttttaaaac cagggctttg gggcggtttt ctgcgccgcc tgtcgccatt ttgctgtcca
     1021 ggcgcggggt taaaaagccg ttgtcgttta aatcgccgtc cgtccaagtc gatacgagcg
     1081 cgcttctctg cctttcgttg tggtcttcgt aaccgacggc ggctgcgtct gtgtggtttg
     1141 tccagtgtga aaatactttg tggacgtgcc gtttgatttc gtcttcctgc ttcgttccga
     1201 ctgctaagcc gttgcccgcg ttgcggataa agcggtcttc cgcctgcggg acgttttgga
     1261 tgctgccgta ttttccggtc agcagacggt acagctcggg gtaagcggtt tcggttacgc
     1321 gcgtgcggat gtcgtcaaac gccagccagc cggtcgggat ttggtcggac ggaaaccacg
     1381 cggtgatgcc gatgtcggta cggcttaaat cgggcaggcg gttgctgttg cccaaggcgc
     1441 ggtagaggtc ggggaaggtg ttttgtgcaa aggtcgtgcc gttggctttg agatagcctg
     1501 cggggtttcg gacggctttg ggaaaggata cgatcgcgcc gacggggatg ccgtccgaat
     1561 tgagtttttt ccatgcggac cagttgtcgt ttgcgctgct cgtttggtgg cgttcgtaca
     1621 cgtcggacgt gtaggcggga taacccaact gcctgcacca gccgtgtttc gtgccggcga
     1681 tgacttggat atggcaggcg gtgttttcaa cgggcaggtt ggagctgccg cccgccgtcg
     1741 gcagggaata gatgccgtct gttttgaggg tgttcaaatc gccccggaag gtttcgactt
     1801 tgaaattgcc gatgccgtat ccggacagtg tgttcggctt actttcgatg tcttcggcaa
     1861 aacgcggttt gttggctttg tggaagatgt cgtggttgtt gtaggaaatg cggttgtttg
     1921 cgccgtgcag gcggagggtg tcgtcgccga agacgatgtg ggcgttgtcg gcatccgcgc
     1981 cgatgtaggc tttgtggccg ccgttccagt tcgtctggtt ggcggataaa tggatgccgc
     2041 tttggaattg ggtttgggcg gtaaaggttt tttggccggt gatggtttgg tcgccggtgg
     2101 taactacagc cagcctattt agtgctgcca gtaattgatt aggctgcgac ttatccggct
     2161 ggataccggc cgcagtcagc acggccagca gctcggattg cacttggttg agccaccacg
     2221 cgggcaggat ggtgcccaat tcggacacgc cgtcgccgtc gtggaaggtc ttgtcggggg
     2281 tttcgatggg gtgcatggtt tagtccttat cggtaggtaa atcggatagc ggtgtgggcg
     2341 ggcttgaggc ggttgaacag gctctcgatc acggcatcgc tgtaatcggt cagcctgtcg
     2401 cccgccgccg agataccggc gcggaatcgg gtaatgcggt tgttgccgcc gcgcacgtta
     2461 acgtgccaca cccacatgat ttcctgcggc gcaagacggt cgccggcgcg gtttacaccg
     2521 gcgcggaacg gctgcggttc gtcgatttgg atttgatagc ccgccgcctc ggccaaacgc
     2581 acaaaataag gaatactcaa gccgcctgtt tcgtttagct tggccatgac ggccaacaca
     2641 cggcgctggc ggtttttgcc cgtaccgtcc aaacctaata cgcgctccca atcggccagc
     2701 atttggccgg cgctgcgcgg gtcgacggca tcggcaacgc tttgagccga ttccgccacc
     2761 gcatccagcg ctgcgccgtc tatttctgcc tgcgcccgca cacgcggggc attgcgggca
     2821 tacgacacgg ggggcaacag gccccgcaag atgtcttgat agctcatgac ggcatctccg
     2881 ttacattaat cgcgcccgct ttaaaccact cgatgcggtt aaccgtatcg gcggcacggt
     2941 tggccgtcgg cgcagtcaga cggcggtcga tcacaccatc cacattgctg atagccgcct
     3001 cgatttgcga cacagtcagg ccgtcgccgg ggatcagggt gtcgaaatat tcttttaggg
     3061 ccgtccgtat gcggcgcttg gcctcgtcca agtcgatgcc gtcgagcttg acttgcacgg
     3121 taacaggcac cgccgttacg gttggcttga gtaccagcgc attttttgcc gttaccgggc
     3181 gcatctcgtc gatataagcc tgtacgcggc gcacagtttc ttccgatggc acaccgtcgg
     3241 cggaggtaat ggcaatatcc accgtaccca agccgcggcg cagcggataa acatatgcgc
     3301 tggttacgcc gtcaacactc aacgcccagt ttttatagtc gtaacggttg ccgcctgcgg
     3361 gcggtcggcg gatgatttcc aacagacgcg ccagcagtga ggcatcgctt tctcggtcgg
     3421 tgccgccttg tacggtaagg cggcattcgg tggccacacc ggcgggggcg gccatcagtt
     3481 gcgcctcgcc gtcgccacat tggcggccgc gcccggctcg tcggcgattg ccggtatttc
     3541 cgccgtgccg ccgctgccga taacggcgcg ggcggtagtg cggtaaaaac gctggccgat
     3601 acgcacttgc aggccgtctg aaagcatgga ttgcgcaata ccgcttacgg tcagcgtgcc
     3661 gctggccgtg gtaggattgc ggcggcgcaa gccgcgcatg gaggcatgcc gctccaaata
     3721 ctcgcggtcg gcggtatcag ggaaaatctg ccgcacaatc cagctttgat gcgcatattg
     3781 cccttcggcg cagctggcca aacggctggc gtgcacataa tggtcgctgt cggggctgat
     3841 atcggcatcc ggccacaggc ttttggtatc gcgcaggata cgctcgcgga tttgctcaaa
     3901 tgtcggcgtt tcaaacacgt ttaaaacctc tttaaatcac gggcacttcg tggcggtaat
     3961 caaacccgcc cgccgccgta tccacccgga tatgcaggat caggcggccg tcgtgcggct
     4021 gctctgcgcg cacggtaatc ttgtcggcac gcccgctctt aacaatcggt tgcagtgcct
     4081 catcggcata ttgctcggcc aacaggctga cccgttgcag gtctttttcg cgctgcaaca
     4141 aatgcagcag cgagccgagc gttttatccg cccaccagct gcccaacggt gtcatcaaac
     4201 ggatatacac ggcattttgc agcgtatcga cggtgcggcc ggtatagtcg ccggtgtcgg
     4261 ggtttagctc tttgtccatg ttggcatttt gccgttttca gacggcctca cggagtacac
     4321 gggcttcaat cccgcacgca caaaaaagca gcccgaaggc tgccaaaggt ctgcttgtct
     4381 atgccggttc cgccggtaag gttttgccgc cgatgctgtc ggtatgcggg tgctggcgca
     4441 acgatatatt gccggccacc acgtcgccgt cggtgttaaa gctgccgccc gtttggttaa
     4501 catcgccgct aaaggtggct ccgtcgccgc cctcgacggc catgccgccg ttgccgttga
     4561 tttggccttg cgccgtcaac actgcactgg tctccaccaa cggagcgtta aatttggcat
     4621 ccgtggccgc attaacctcg tattgtttgc agttaacccg gtacacgtcg caatcggcct
     4681 caatgatttt gccttgctta atcacgattt ttgcaccctc atgattaaaa atcgccgtct
     4741 cgccgggctt aaggtttttg atgcggtagc tgccgtgctg gctgcacaca atcacaccgt
     4801 gcgaagtatt gccgcccagc ggtatcacta ccgcttcgct gccgtcgggc ggatggctgg
     4861 caaagccgta ttcctgcaaa tgttcaaggt cttgcagggt ttcgtcggcc aagccgctca
     4921 actgcacgcg ctgtatcggc tcggacgaca ccaccagcgt gatttttccc cgaaaggccg
     4981 cgcgcagggt ttcgccgata tttttagcag tttgtgccgt ttttttcgcc aatttactca
     5041 aactcattca aacaccgccg tttctgcttg ttttttgccg ccttttttgc ccttatggct
     5101 cacgcctttg cgtttgcctt tgcgcttgcg cgccgcctcg gcctttttgg ggtaagcgtc
     5161 gggtgtccaa ataccgtcct ctttaagccg cagctcggtt tgcgtgccat ccatgcggga
     5221 tagcataaac cgccgcccca tcagaaaaaa caccgcatcg ataccgtgct cgtcgtcgat
     5281 cacatgcaca cgctggccag gttgccacaa tacgccgtcg cgggttttat ggccgcccac
     5341 ggttatcgtg agtgtaaatc cctccagccg ccagtcggcc agctgctttt tagcctgctt
     5401 ttgcaatgcg gccaaattgt cggcatcgga caccaccacc gttttagggc ggtgcagcgt
     5461 catcgtcggg tctttgtaca cccactttaa atcgtgtttg gcgctgtcgc cgctgcggcc
     5521 gtgcgattgc gccaaaaaag taacctcgga aaagcggttg tcggtatccc actcaatgtc
     5581 catgcgctcg atattgcagc ggctgtcggt gcggctccaa cacaatgtcg ccaccggcgg
     5641 gctgctgtaa tccgcaccgc ccaccaccaa cgtgccgtcc ggctccagcc acggatgcag
     5701 cccgaccgag ttggcaatat gggttaatgc ctgccatacg gtttcgcccg gctcgatgtc
     5761 gattttgtcc aaagcggggt tgttttcggc cttaagcacc accgctttaa tctgcggcca
     5821 cggcgcggcc agctttttgg ctgcatccaa taccgtcatg ccctttacat tgagctgcgg
     5881 cgcggagcaa tccaccaaaa aaccggccaa atcacgcccg ctcaagctca actcgcggct
     5941 gcccttgctt ttgccgtggc gctggctgcc gatgatgccc gtcatcacga tttgcccgtc
     6001 tatcactacc tcgcagctct ctccgcttaa atcgggtatg gccgcctccg gccccaacct
     6061 gccgatgaca aaatcgaagc tgtcggcagg gattaaaaag tcgctgtcga tgtcgtagcg
     6121 ctcccagtgg cggtgctctt taccgcccac gcgcaccgac acggcatagc cgtatgaatt
     6181 attttgcata gctgttgacc aaagtgccgc gcttgataaa cgcggggtgg tggatatggg
     6241 gattgagccg caccagctct gctgcgcggg ccatatcgcc gtaaaactcg tgggcgattt
     6301 ggtgtatcgt accgtcgatt ggggcttggc gcacaatcag cggcggcttt tggttgatgg
     6361 ccgccgcaac caacgcattc agacggcctg ccgccgcgcg cagggattct gccgtttggt
     6421 aagcctcggt atacacggcg ttggccgtca gcccaccaga ctcggcagca gccgtctgca
     6481 ccgcccgcaa ggcggcaatc tcggcctgca tacggcggcg catggcgcgg ttaacctcaa
     6541 tcaaatcggg cgcggtcatc tcttcgccat gcgcctcgat taatgccgtt gccaccgaca
     6601 acagcgatga cgtggataac aggcgcaccg cctgcgctac cggctgcacc tgtttggcgg
     6661 ttaaccggtt caggcggttt tgcaggccgt ctgaaaagcg gccggtcagt aaattatcag
     6721 ggatagcggc ggcacggtcg gcaacagcca cagccccgtc aaaccgctgt cgcggcgacc
     6781 aaccggcatg gtgcatggca ttatcggcca aacccgcctc acggcgtatg ccagtatcta
     6841 ccatgaccga tatatccgca aacagcttgg ccgagccgtt tttaaacgcc gctgcgctgt
     6901 atccgccccg atcgggaaag gcaattttgt ccaaatcaaa caaacggcgc accgcaccaa
     6961 acgtacccga tgccgcactc caaatgccca gcgcgctgcc ccataaagcc gatacgcccg
     7021 catccaccgc caacaccgca tcaacaaagc cgatagccgc ctcgcggtag gtgtcgatat
     7081 cagcaatcaa cgcctcaagc tcgaccaaaa aggcgttttc aaaaacaaag atttcctgcg
     7141 cttcggccgc ctcgcggaaa gtaatatcga tgcccgcata atccacataa tcggcctcat
     7201 gtcggtaact ccatgatgcc gcaatcatgt tgtgcatccg cccccaaaca gggtgcacca
     7261 gcacgccgcc gcccggctgc tccagcgcat ccagcagctt tttcagacgg cctgcatagc
     7321 ccttgcccca aaacaccgca ttaatctgca cctgccgccc ggtcatgccc atgtcttcaa
     7381 ggtcgatacc ctgcacaaac ggccgcgcat gctcggccaa tgccttgccg ttgctctcgt
     7441 ccaccacctc aatatcaaag ccgacgccct tgtaagatgc gtcctgcaat aaggtatgcc
     7501 atccgctcat tattgacccg ctccacggcc gaacatggcc acttgatacc gcgatacctc
     7561 attcgcgata acacgtccgt cgatattcag gctcacattg gtattgatgg tctgacttgc
     7621 cgacgccatc gcactttgca cttgtgccaa acctgttgta actgcagccg tatcctgcgc
     7681 caatgctgcc tgatacgccg ccgtctgctg ctgaatggct gcctgatacg ccgccgtctg
     7741 agttgaaaac aacggagaca acatcttatc ggtaggctgc gctgacggag cagccaaata
     7801 ctgagggaca ccaccccgcg cctgttcccg cttcggcgca ggttcaaatc ggataccgtt
     7861 gtttttcaga cgattaatca tttcaggagt tgtcttggta ccacgcaaac cctttggcaa
     7921 tgtgccgtca cccaaagact cggaatacag caacatacct aacaaacccg cccgtttaac
     7981 taacgctgga ttattcatga gcccgctacc ggcagatttt ccccatgaca gcaacttacc
     8041 gcctgccgct gtggcacctg ctgccacgcc gcctgcggaa gccttacccc ataccaacgc
     8101 tttactacct acatctttca gaaagcctgc acctttaccg ccaccggcgg ttaataacat
     8161 tgcggcagac gccgctgttg ccgccgtcgt caatgcttgc agggctagag tggcattagg
     8221 gtattccgct gccagctttt taaacgaggt ttccatatcc accaatgttc ccgtcagact
     8281 ttcccgcaac attgccagcg attcctgttg ctcttgttgc gccgctgccg acaacattcg
     8341 cgcctcgttg ttcttagcaa tttttccgtt atccaacgtt acgccagcca acgaagccat
     8401 atattcacgg atttgcgtca tatccgttgc agccagcaaa ccttgttttg cctgcaaatc
     8461 aggcagcagt tgcgccaaaa gcgcgccctt aagcatattt gcctgctccg ccgccgtctt
     8521 atcgcctgca gccgcgcgtt tcttataatc ttggtattgc ttatccttta ctagcatggc
     8581 atcggcaaga cgggacaaca cctgcactgc gttttcgccg ttttgcttgc cttgcacaac
     8641 cgagcctatc caatcgacac ctttcttcgg gtcatttgga tttgccatct tcttcagacg
     8701 acctatcgtg taaggcgaca gagttttact caaaagattt tgcacattag tcgccgcttc
     8761 ggcaggactg cccgatttat tcgccgcaga ttgtaagagt gagagcaggt agtccaaacc
     8821 gccgacacca ttcatccctg cctgttgcgc ggcagggagc aggctcggca gctcccgaac
     8881 catatcccgc acctcgaaag tgccgtctaa acccgattgc aagacgtgct caagcccgag
     8941 ttgcaggtct ttaccgctca tgccgccatc tttcagggtt ttaatcagtt ttgccgtatc
     9001 ctcgccactg ccttctgagg caagtgcaaa agcatatgcc gcctgcgctt cattcttggt
     9061 ttgggcaaaa ttcagactgg tggtcatcat gccgctgatt aaatccaaag ccttatcgtg
     9121 ggtcccgcca tttttctcga caagttcaag tgccaaatcc ttgatctgtt gcgcaccttc
     9181 agttgcaatc cacgctgccg atttactgtt atcctcaata aatgcctgtc tggacacgcg
     9241 gttgatgttc tcatcaagct gctttctgtt gtccatagca ggcttaagca ccgtatacgc
     9301 tgccgcgcca ccagccaccg cagctgcacc gaatcttccg atttttccca tcttgtccgc
     9361 aaatcccgtg ccctgtttca gttccgcgtt cagctcgcga attcggttac gcgtagcgac
     9421 cgccgcccgt gccaaatcat tttgtgatgc cttgccgctg cgcgccaaac ggttaaacgc
     9481 agcctgtgtc agcatgattt cgcgttgaat ctgtttttct gagcggatgc cggtcaacgc
     9541 ataagtatgg ctttgccgtt caagttgcgc ccgcgatttc gcggtacgat ctaattgtcg
     9601 ttgagtatca gccagtagcc gtctcgctcc gtcatctcgg gcggttaaca ccaacgacag
     9661 tttcatgttc ccgtttgcca taaaaagcag cctttaaacg tattaactca aggtcaatta
     9721 tcgtttaaag gctgtttaag ggaggcggta tgggcttcaa ccccgttttc gtgctttatt
     9781 tgtttgttgc gttataatac cagaatgata accattttat tcaaagggat attttatgaa
     9841 tatgaaaaaa ttgatttccg caatttgtgt ttcaattgtt ttatcagcct gcaaccaaca
     9901 atcaaaaacg gcacaagccg aagaacctgt ccaaagtatc caggctgctg attgtaccgc
     9961 cccaatggac atcacagttg aacaatatct catcaatttg gagcaagcat ttaaaactca
    10021 gaacgtctca acaaaaatcc ataataaaaa tattgtcaag accgattgtg gttatgacct
    10081 tactttggta atggattttg gggcgattgc gctcaaactg gacgagcagc aaaaaattag
    10141 agctatctca gtaggctaca ttttaaaaac cgacggagag aaaggacaaa atctagtcaa
    10201 taatgccata aatggattac acagtattca ggcagttctg tctttaacta ccacagacaa
    10261 attgggcgaa tcggaagcag gaaaacaact tcgtcaaaga atccaatcag acaggagcaa
    10321 cagcgcaaaa agacgttccg gcagatggta ttttatatag cgttgttttt gaaaaagaaa
    10381 caaacaccat tgcaataatc ggcagaaaac aaccctaacc gtcaaataaa aaaaaggtcg
    10441 tctgaaactc agacgacctt tttattttcc ctccggcttt ctcagccgtt tcgacacaat
    10501 cactccctct tccttcggcg tttttatgcc caggctcgac aggatgtctt caaaccatgc
    10561 cgacagctcg gcgtggcaca tatcgcgcac tgcgtccgcc cctatccccg cctttgccat
    10621 caggatgacc gcctgacggt agcttttgtg tgccgtcagg tagctgcgtt ttactgctcc
    10681 gccgccgacg gcgcgtcggg gctcgccgta gcggcggacg gttttaggca caaatcctcc
    10741 tgagccgcca tcacggtatg gtagtcttcg gtagagaggt tttccatcag ccactgcgcc
    10801 gtcaggatgt cggatgggat accttgggct tcaatctgct gcgaccagta ggtcagcgtc
    10861 tctaggacgt cgcgttgcag cagttcggct tttgtggcgt tttcgggcag ctcgggcaag
    10921 tcatcaatca gggcgaacgc cgccagttcg ccgccgacgg tcagtggctt gaggcgtaat
    10981 tcagatacga ctttgccatt gtaggtcagc ccgtgggtca gtttgacggt aatcacgcat
    11041 tactccttga ttttgtgcaa agcaatcatc tcaatgtcga tgcgtgcctc gttatcgact
    11101 tcatattgct cgctggtatc gacggtaaag cagtcgaggt aggaagtgcg gcgaccttcg
    11161 tcgttgatgg ggtaaatcgt gattttcgcc ttggtgatgt tgtcccagtc gatttccgta
    11221 ccgtcgatcg gaatggcggc ggtaacgctt aatttgtgtt cggttacgcc gtcacaataa
    11281 ccgttgactc ggccgttgcg gttcatcgtt ttgaccggct tgcggcctgt agtggtttgc
    11341 ggtttgatgc tcacaatctc gatatcgcgg ccgttcacct ccataatcac cgcgccggta
    11401 taggtagcgt cgctcatgct ttatcctttt ttaaggtttc agacggcctt taaacctgtt
    11461 ttaaacgccg tctgaaaagg gttacaaaat caaatcaatg cgcccggcaa agacgtgcag
    11521 gccgttgacc acatcggcgg ggataatggc attaacacgg ttggggtcgt tttgcgcacg
    11581 cgccaccacc agcttgcctt tgttggcctc ggcgttttcg atgatttcgg cttggtcgag
    11641 cttaatcagc acgtccaaaa tctcgctctt aaccttgggc agcaggcggt cgctcaattt
    11701 gtcgcgcgga aaacgcaggg caatgcgctc tttaacgctg cggcgcacat aatccagcgt
    11761 gcgaatggtg gtaatgtcga gtagtgccgg gtcgtcggtg ttgttggccg acttggtata
    11821 ggttgatacg gcacgcataa tctgcacgcg gttgttgacc gctgtgagcg gggtcaagcc
    11881 gttgtacagc gcattgttgc attctgcaaa ccacggccat tgcgcatcag gtgtaacggc
    11941 cagccctttg atttccagcg tgttcagcgg cttggcaggg tcttcttcaa aggccaacac
    12001 cgccgcataa cctgcggcga tgatgccgtt tggctctacc gcacctttgt accaagcaca
    12061 ggtaatgcgg ccgtcgttga ttttgccggt agcggtggtg gccgtgctca aggccgcact
    12121 catacccaat acgccgatac agccgcgctg ctcgatggcg ttgaatacgt tggtaatatg
    12181 gttgctcaag gctttggcgt tggcgtcatc gctaaacggg ctgcaaataa tgtgataatg
    12241 cttgcccgcc actttggaca aggccgtggc aatgtccgca tttttggcac cgccggtaaa
    12301 gacattggct tgataagtca ggccggtatt gccggtgctc acggttaagc cgctctcgtt
    12361 gccgatctcg cctttgtgtt tggccgtcag cgtaacggct acgccgctgc cggatgcggt
    12421 aacggttaca tcggcggcag tgatggcggt tttcagacgg cctgccactg tggcggcggt
    12481 ctcgccggtg ttaacggccg tgcttacctg cttgccgcca atcgtgattt ccaccacgcc
    12541 cggcgcggtg gccgtgccgg aaagggtaac ggttgcggtt gcctgcacgc ctgcgctgtg
    12601 gtcggcaata ccgataacgg tcaaatccaa ataagggttg ttggcaaatg cttggcgcac
    12661 catcaaatgc gccagcgagc cttgtccgaa caaatcggcc gcctcggcat cgctaaatag
    12721 ttgcaccggc tctaaggcgg gctgtatgcc cgcggtcagc atgggtgcaa ccatcaatac
    12781 cttttgcgga ttttgcggca aaccttgcac ggcattgcgg gtgttaaatt caatatactg
    12841 cccgggcacg cggatgctgc ccggaatcgt gtcaaaatca atatggggca tcattactct
    12901 cccttagtat tgcgggtttt ggtatttggg gcggcatcct caaccaccac caaatcgccg
    12961 tcatcaatca tgcggcggta atacaggctg ttgccgtcca cctccaccgg ctcttggccg
    13021 atatattcgt gcgggttgtg ttcggtcggc acacgcaggc catctgccgc cgttactttg
    13081 attttgctca ttgcttatcc ttaagggtta ggtttacagg tatttcatcg gcggattgcg
    13141 ggtcgtaaat tttgccgtcc aacccctcga aatcaggcca cggctcgctc aatgtaccct
    13201 gatacttggt aaagatatgg ttggggtcgt cgggattgtc ggtgcgttcg gggtagcggt
    13261 cattttccaa cccgcaggtg ttaaagcgga tggcatactc aaccgcatat atgcttactg
    13321 cggcgttttg caccagcaca tgattggcaa tcgcgcgcac cgctttgggc accaagccgc
    13381 ggctatcggc aaaaccgagc cgctggccgt caagcaggcg gcgaacagcg cggattaaat
    13441 cgttgctgcc gatttcgcgg ctgtcgatgc cgccttgccg ctgcgcctgc tcgttgcgca
    13501 gattgcgggc cgccaccatc accacaaatt cggcggtatc ctgataacgt ccgcatacgc
    13561 cgccggtgct ggcaggctca actttgctgc cgccatacgt tacccaaacc gcaggcagcg
    13621 tatggatttg ccccgccaaa tcgtcggcct cgccgttgta gcttttaacc gtgcgcacca
    13681 tgcgacccaa gccccgtttc agacggtctg ttatcgcttg ctcaatatca gtgatcacgc
    13741 ccgaacacct tatttttacc gtttacaaac acaataccgt cttcgcccgc ggccacttct
    13801 tcgccgctat catccacgcc cagctgcact tcgcctttgg ccatagcacg cagcaggtcg
    13861 agcacgtcga ttttgtagcg gttgcggatt tcgtcggtaa tcaacacgcc ctgagccgcc
    13921 gtcagacggt agcgggcaat gtcgcagcaa aggcgcacca agatgggcgg cagatcctca
    13981 aaaggtcgtc tgaaccgccc cagatacgcg tcgatttcgg cagtggcgtc caccagcgcg
    14041 gtttgtgcga cctcgcggtc aatcagcccc tcgttgttgc ggtcggtgag ctgcaagact
    14101 tccagctcac cgaaacgcgc aaccatatcc tcaaccgtcg cgtatgccat tactcgaccg
    14161 ccttgcgttg cagcataggc tcggcgcaga ttgccttcca caccgcttcg ccgacttcgg
    14221 cgcgcttcac ttcacgccag ccgccgtcaa acagcaggcc gccgcgccaa aattctttgc
    14281 cgtctgcgcc ggtactgacg agcatcacat cgcggctgtc cgccaaagcg tcggcggcgc
    14341 gttgcgtatg ctgcactttg agttcggcaa gttcggcgga cagtgccttt ttgtcgtctt
    14401 cggctttttc caaggctgtg gtcagcattt cgacatcgtt tcgggcggcg gcaagctctg
    14461 cctgcacggc gtccaattcg gctttgatgt cttcaaacga cggggcggcg gtttcggcgg
    14521 tttctggttt gttgttggtt tttgccatga tgactccttg tttcagacgg cctttaaata
    14581 ggtgttaaag gtcgtctgaa aacggttcgg ggttacaggt ggacgcaaac gtgcagtttc
    14641 agacggcctt tgaaggtatt ggtcgtgccg ttgattttgt cggcttccaa taattcacgg
    14701 gctttgtctt ccagctcggg cggaacgacc aagaggctgg gcttcacatt caacacatag
    14761 ccaccgtcgg ctttgatttt ctgcattttg acgataatgg cggcgaggtt ttcgcgggtc
    14821 agcgcggttt tttccgccat gtgcatcagt tgccacaaac cgaaaccggc attgcagcgg
    14881 cggcgcgatc cgtacaggta ttcgtcttcc ataaacacgc ggtcggattt ggacgggtcg
    14941 aatttggttt caaactcgga gtcggtgcgg ttttggaaaa tcagcggctt cagggtcttg
    15001 gtgtcatcga cgacgtagaa ggtcggcgcg tcgttgtccg taccggtcgt cagattggag
    15061 tgcggcgtat tctgacccgt gccgtccgat ttctcgtaaa cggggtggtc ggtatcgaag
    15121 aaatattggc cgtcataacc gacagtggtt ttaccttttt tgagcagacc ccacaccagc
    15181 gtatcgggca gggcggcggc ggactcgccc attgccgcca tcatcgggcg gtacatgccg
    15241 acttggtcgt cttcgatgtc cgtacgcgct acgccgacgg ttgcttcaaa ctttttgttg
    15301 tcgaggctca tcgcctgctt gttcattttt tcgatttggc gcgagccgac ccattcgcgc
    15361 atttggggga atttgccgag ccacgcataa gtattggttg ccgtattgga cggaatggtc
    15421 atcgcaacgg cggtatagtc gggcttgacg gattccaaac ccgtttgaaa ttctttgcgg
    15481 aatgctgccg tgattgcggt caaaatcgct gatttgtcca ttacttacct tcgctttctt
    15541 tgatttttac aaattcttcg ccggacatgc ccagcatttt tgctgcggct gcctcttccg
    15601 cagtcagtgc ggcgacgcgt tcgtcgggtg ctttaccgcc cgtttgcgag cctgccagtg
    15661 cagcgaccgg ctgggcgttt tcaataaagc cggtcaaaaa tgccaagccg cccggctgtt
    15721 tcaatacgcc ttctgcccac tccttctgag caggcagcaa tttgcctgaa gtcagcgcgg
    15781 cggtaatcaa ttcgttgcct ttgtctgctt cctgcttggc agtcagcgcg gcgactttgc
    15841 tttgcagctc ttgaaccact gagataggcg cgtatttagt caaatcaggc ttggcggttt
    15901 gcgccgtcaa tgccgcgatg cggctgtctt tttccgccag ctgcgcgaac acgtcggcag
    15961 acaatgccac gtctttcggc ttggcttcca cgagcgcgga caatgccgcc ttcagttctt
    16021 cttcgcccgc atcaggcaga ccgaacagtt gctgtaacaa ctctttcata gggttttgct
    16081 ccgtttccga ttttaaaatt tgcgccgacg ctgccgccag cacctcgtcc ataccgtcca
    16141 acgcggggaa atttgtcagc gcggcgtgaa aaattttgct tacatatccc tttgtgtcat
    16201 aggaaaacac agcagagatg tagcgatact cttttgcggc aattgccgca gccgccttgt
    16261 ccgtccactc cacttcggca aacatgcctt taggcgtgaa ctccagccaa cgcatccaac
    16321 cggcggcagg tgcaggttgt ccgtttttct ctttgtagag cgtctggtgt tcataatcga
    16381 caaccaactg attgcgcgag ctgttggcca acaacgcgac atcatgaccg ttttcttcgg
    16441 tcagatacca cgcagggaca tcagtcggac gaccgtctac tgcgcgaaat tcgccatatg
    16501 gcagcagttg gattcgcccg tcttttggct gcacctcgaa actgcacacg gcaagtaggg
    16561 ttttttgtgc atttttggac atgtgtacct ctctcaatac ggtcattttg cctatttgaa
    16621 aaaacaacaa agagtaggcg tgcttcaaac cctcccgcca tttttttaaa acctgtccgc
    16681 gcgatacaca attaatcgtt ttcaggggct ttttagaagc gtctaggatt gatttttata
    16741 tcttgggcag gggtttgtat tacccgcata aaaaaacgcg ctaaaaagcg cgtttttgag
    16801 ggttttataa attcacggta tcagacccga aaaataatcc tgcacatcgt ccatcaaagc
    16861 ctgtttgtcg tcgtccgtca gcgtcaaaaa ttcccgttgc ggaatccgaa ctttgcggtt
    16921 gcgccccgcc ataccgccga agttgtggat ggcggcatag acgatattcg taccgacaag
    16981 ggcggtatcg ttgtctgaca gtgtggaaaa actgtctttc agacgacccg aatccgaaag
    17041 cggcttgccg tcgcggtatt ttagccccaa ccatttcgga cggcctgcgt agcggaaatt
    17101 gagcttgacc gccgtgtgca tggtttcgga cagtcggcgc atcagcaggt agcggttttc
    17161 gatcccgttg ccaagccgct cgatttggtt taggacgaca aagatattgt ctattttgac
    17221 atcaatcatg gtattatcct gcctaggcta ctaaaaaagc ggtgaatttg tctgccctcc
    17281 gcagccctta tgcagtcctc ggactgggat tggtatccgt tacataagcg agagtgtgtc
    17341 gggattgcag tccgacttag tagccttttt tcatttcata gcgtttctta cttttcacaa
    17401 tttctttttc atccgccctg cgaaacgaat ccaaaaaaat cgaatcgtac tgtggcagat
    17461 atttcaaaac tgccatcaat ctgtattttt cgttcccctc caacggtttt ccgagcaaaa
    17521 caaaactttc ttttcctctg cttttgtcat aaaaaatata ttcgggctca tgaatcagat
    17581 cggggagcat ggcatataaa tctgttccaa acgattgccc ctcccgactg tccacctgtt
    17641 tgaccaatgt atcatcagac agccacaccg tcgctcgtgt catacctgcc aattcttgcg
    17701 tttccttgct caatacaccc gcagcaaatt taagctgtct tgatagcgca tttcggattt
    17761 tgattttctg ctctttatcg ggcttgccat caatatccaa acgttgcttg acttcataaa
    17821 actctttttc aagctgttta aagctggttt taaaatccgc accacccatt tccgctttgg
    17881 caaattgatg cgccaacgca cggtcgtact tgtctaaatc ggggcggtag ttcattcgtc
    17941 cggcgttgta atcaaatcct cgatcggtcg tgtacagact gccatccggt gctttatagg
    18001 caagggtcag ataagtatcg ccttttttgt tgtagatttt atgggtctcg actagattgt
    18061 ccgccgtgct ttgcccgaca atccgcccct ggcgttccac atcccgctcc gacagcgcga
    18121 tgaccgagca acggcagttg tagccgttgg gcgggtaaaa cgttgcccaa aacgggtcgt
    18181 cgtagcggta caccagcccg tctattgccg aatgcgccgg acgggtgcgg ctgtcgccta
    18241 ccgcgtcata catccaatag ggccgtgcat caatatttgc catatatcct tgatattgac
    18301 cggcgttgta ggcagtttgc atgttggtac ggtaaatcgt ctccaacctc cgcggcgaac
    18361 cgaatacctc gccggtggct gggtcgatga tatccttacc gttatgcccg ttcggatgca
    18421 gccagccctt gttgccgagc agattcaaga taccttttcg ccaatcgtca aacgactgtc
    18481 cactttttgc cgattcaacc atcgaagttt tgatgtcgtt gagcatatcc aagtcggtca
    18541 tgcgggcaat cgtatagact ttggcaattt cggaggctgt cagattgcgg tagctctccg
    18601 ccgtaacctt tttactttcc agccactcga ttgccttttt tggcggcaga cttaaggcaa
    18661 atcccaaatc aggcgcgggc atggtcttgt cccaaaatat cgctgataaa caaggcctgc
    18721 tgcatatagg tacgcagttt cgcgttgtcc aaattcggat aaagcgcatt cagtgcggca
    18781 tctgcctcct cgtagctgtt gcaagcatta agtgcggcaa ccgcctgacg caccatcggg
    18841 ttgagctgag aattgaaatc gggctcaacc agcgcgtcat ccaacgcgcc gtccaatatt
    18901 tcctgatgcc tgcccgtagc cttagatggt acggtgtggg cggataaagc cgccaatgca
    18961 gttctgttta tcggattgtc cggtacctgc cgcaccaaca cagcctcacc ctcctgcaca
    19021 tctggaatga ccagtttgtc gcgcacccag ctttcgggga tttgtacgcc gacatccacc
    19081 agtttcggga tagcgtcggc aaagaccgcg atgtctttcg gctcgcgcgt gtcaaattca
    19141 aatttcggca cgcggtttgg gtcggcattg ggatagttga tttgcaggaa cggtccgatg
    19201 atttggcttg tgatggtttg cgccacctgt tttgcgtccg acaccagcaa atcgcggcgt
    19261 acctcgttgt ggatattgcc cagcgcgttg gtgctggatt ttccgtccgc accgctggtt
    19321 agcgtttgcc ccaaaatcag ccgcgccgcc gatttttcgc accagtcggc catctgcaaa
    19381 aacggattgc tggttgccat cgtaccgctt gcagcgttgt gcagctcgat ttccatacct
    19441 tctggcatga tgcctgccgc gttgtgaccg atttccgcca ccgctcgaag cagggtgttt
    19501 ttttcctctt tggttgcgcc cgcgccgtat ttgccgatac ggatgggcat gccgtacagc
    19561 tccaaaaact cggcaaaatc gtggacggcg tagtgtttga acatatacag ccaggaaagc
    19621 gtgcggaaaa gcccgttgcg cgcctgctgg acgctgcgcg atttttgggt atgaacgacc
    19681 cagcccagcg gccacaacgc ttcgccttcc ggattttcgc gggtacgcag cagcagcccg
    19741 ttgtctttgt cccatttgaa ccagctttgc gggcggtgga taaagtttcg gggtaggtaa
    19801 aggccgtctg aaaataccca ctcgacctcc aacgcagaaa atccgtgccc taccgcgtcc
    19861 atcaaatcca taatcaggtc ttcgagggta ggcaggctgt ccatcatttc gtaggcttgg
    19921 tcggacagct tttcttcttc gggcgtcgca tttcgcggcg gggcgacgcg ccagttgagc
    19981 gtcagcagcg cgcgtttgcg cgtccccata tttgccgcga tgtcgctgtc gcgctcctca
    20041 atgtccgcga aaagctcgtg ttgggcgcgg atgtcgccgc tttccgcatc ctcgaagagg
    20101 gcccgcatct tttgcggtgt aataaaattg gacggatgct cggcgataac ccgaccggtc
    20161 gccgtaattt gagccacgtc cgtctgcaat gcagcttcgg gcttttggat tttagtttta
    20221 ttgttctttt ttgccattat cggctccatt tactttttat ctcgtcatca aaatcgttat
    20281 cggtagggct ttgccattct atcggcacac aattggccac cgcccccgac cagagcatat
    20341 gcactgcatc aggaccatca tcatgatcag cctttggaaa atggcggaac tgctgtatca
    20401 gcgtctgttg gctctcattc aacagaatca aaccgttcgc catgtgaggc tgtaaagtct
    20461 caatccgcaa gagcttgtcc gataccggct tgaccgcccg cgccgggaca ggtattccac
    20521 gcgccgcgct gcgcttgacc agctcatctt tcaaaaattc ctgaaactga accgtctcaa
    20581 caaaccacag tttgcaacga tattgacggt gcaatctgat aacgtcctca ataatcaaat
    20641 cagggagacg ttttttaatc tgagcttcca cgacatacag tttgccggtt tcacgttgat
    20701 aaccgccaat gataatcgcc gacgggtcac ggctcgcccc cgctttaccc agcgacgggt
    20761 cgagcgcacc gaaatacacc aaatcggacg gcaggtcgtt ccaaaacttc atcgacttgg
    20821 caaacggcgc atcttcgccg ctgaccgggt cgttttgata ctcgctgtca aatgtcgcat
    20881 gaccgtcacg ggcgcggatt ttcatcagcg cgagtacgcc gcgagccgcc cagcttgttt
    20941 gcgcgccgcg ctccatctcg tctttattgg cgagataaaa cgcctcggcc accgtctcgc
    21001 cgtcgtttcg gaaaagttcc tcccatctgt cccacaagtc catgcggtcg ggccagcgtt
    21061 tcatcgcctt aaacttaaca ccccgccaaa acggattatt cagggtgcgg tttagcacgc
    21121 tgtcgtaatg caaaatcgtg ccgatataaa tcacatcgta tttctgaccg acaccgccca
    21181 aaggcaatac agtcttagtc agccacgcat tgagcttgtc gcgctgttcg gggttgcgga
    21241 cttgctcgtc attctcaata tcgtccaata tggtcaggtc aggacggtaa gggccgtgac
    21301 gcaaaccgcg cagcttttta ccgctaccgg ccacttggac tttaacgtca ttggccgtcg
    21361 caatcgtacc ggcctgccat acgcggccct gcccgcatac ttccggaaag tcggttttca
    21421 agcgcggatt aaattccaat tccgccttga tggcttcgag catcgggtat gcctgatcta
    21481 tgctgtccat cacaataacg gcataatgtt tttggcctgt cacaatacac cacagcgtaa
    21541 acaactgagt aacctgcgtc gacttaccct cgccacgcgg cgcacccacc gcctcatttt
    21601 cccctttggg ggagcggata atctccggca gacggctgaa taaaaacgca tgcagttcgg
    21661 atttttcagg ggagcggata taatgcggaa agtaggtgtt gacgaaatac tcgtaaccgc
    21721 ctaccgggtc aaacaccttg gcacggcgtg cagcaatagc cttgggcaac gcatcaaagc
    21781 cgtccacttc cgcttcgata acttggcgca aactggcggc cagttcggca agggatttga
    21841 ggaattcttt tgttttcatg agtatttata atgactggat tcatttctaa cgcatttata
    21901 ggcctaaaag aagcaggatc acttttaaaa gggtttaacc agctcaaaac agaggcagag
    21961 gtagcgcaaa aaaccatcga acttaatcaa atcattgctg gtattcaaac tgatttattt
    22021 gaagcccaat cttcttatac ggcggcaatt agccgtatag atgagttgga aaaagaactg
    22081 gtggcagtaa aaaattggtc tacagagaag caacgctacc aattgtatga acttgctcct
    22141 ggttctttcg tctatcgatt gaaaacagag atggcgaaca gcgagccaat tcatgatatt
    22201 tgtccccaat gttacgagca gggtgtcaaa tcgattcttc aattcaatgg gtatgaaggt
    22261 gcttttcata aatattcctg ttcccgttgc caaaccataa tattgggaga gcgtttaaag
    22321 tcagatgttg aagtattttc agtaggagga tcacgttatg ggagattgat agacggctat
    22381 taaccaaact tcttctccac ttccgcccca aacggctcca atacctccac aaaggcaggc
    22441 aaatgtttgg gatgtttttc ttgcacaaac gccatcaaga actcaatcaa ttccaaagcc
    22501 gtcgccagtt ttgacgtttc cggcatcaca cgcgcattgg ccgatacggt tttcgtaaac
    22561 gcatcggcca ggctggccaa cagcttggcg cggtcggacg ggggcaaatc ttcggtactc
    22621 gaatcctgca gcatcgtcat cgtgctgttg tactgcacca taaaaccggc caacatcgca
    22681 cggctcaagt cctcaatacc gccgccggcc aaagtgtagg cggcgcgcat cttatcccag
    22741 tcgtcgcctt tttccttatc cgcacgtttc cacgcacgcg cagtggtctg cgggatttcg
    22801 cacatcaagg ccgccgtttc caaagtttgc tcgccgctca catagagccg acgtaacttt
    22861 tcacggattt cttgcgggtg agccataatt acagtcccat tttcgcttta agcaattccc
    22921 agccgaccgt aatcacgccg ccgcccagtg cgccgaatgt aatggccgtg cgtttcgtgt
    22981 cttggcggat ttgcgcaatt tccgcctgca tttctttctg atttttcagc gtttgatcag
    23041 tcttgttttc aatacgcgcc aaggcttcca aaatcgggtc gctcatgatt tgtctgcttt
    23101 cctgtccagt ttttcgttta ctttttccaa cttgttttcg atgcgtccca acgaagccgc
    23161 aatattgtcg cggtctgcct tggcatcttg cttggtgtga taagagagct tgaccgcgtg
    23221 cagctcctct ttcaggtcgt cgatacgctt gtccgcctct ttcagacggc ctgaaatgcc
    23281 gttgacccaa aaccaaaatg ccgccgtcgc aatcggccac agggttctga aaccaaattc
    23341 aaagtccatt taaaacccct ttaaaccggc acatcgccga atacgatacg gacggcgtaa
    23401 ccgtcagggc gattgctggc aatctccagt ccatcaccat cattacaaac acagtaatac
    23461 gcccccaaag tttgccaaac cgcacgttta aaggtgtcat agttcgtaat tggatattca
    23521 aggtgaaacg tcgtctgaaa atccttatcc atgcgtaccg cgtactcaat gcctgcctta
    23581 tccaacagat cggaaacatg aatgacaaac ggctcttgct cgcgtgcgcg gcttaagccc
    23641 aattccaaat cggcatggcg cacagccaac tgacgttcaa ctaattcacg gtaggtcatt
    23701 ctttgatacc cattaaatat tttgtccgct tgtacaattt cttaacccac gaaatattta
    23761 caaatgtata aacctttgct acaacttcgc cgtcatagtg cgcattttct cgtgcagccc
    23821 gaaatttcgc cctggcttct tcagggttgt ccgcccaaat gctcaatgac caggacttgc
    23881 cgttaaagcg gtaagaaaac gtgtactcat tcataggaga aaccttatgt attttgaaat
    23941 ctataaagac gcaaaaggcg aataccgttg gcgtttgaaa gcagccaacc atgaaatcat
    24001 cgctcagggc gaaggctaca ccagcaagca aaactgtcag cacgcagtcg atttgctgaa
    24061 aagcactacc gccgctaccc ctgtaaaaga ggtataaaat ccgctttcac cctcagcccg
    24121 cgccctacgc gggctttttt gtcagtttcc gactttgcgg gagtgattgc ccgcccagtc
    24181 gcgccaagcc tgattttgat tttccaactc cgccacatag ccgccaaact cggcggcgtg
    24241 ttccaacagc gtggccgtct tgccgtcttt cggcggattt gggcgtaccg gcgcgaccat
    24301 caatgcggca ggcggtgtcg gcatgaccgc cttttcgaca accttaattt ccgtagccga
    24361 gggcgcgctt gtagagctgc aagccgtgat ggccaaagcc gtcaatacaa ccgccgcctg
    24421 catttttacg gtcttgagta aggacatttt cgatttcctt tttattttcc gttttcagac
    24481 ggctgacttc cgcctgtttt ttcgccaaag ccatgccgac ggcgtgcgcc ttgacttcat
    24541 attttttcgc ttccgcgcgc gcctgttcca gctcgcgtgc atagttttga gccgacaaca
    24601 gcatggcttg cgccttgtcg cgcttcattt tctcaatgac cacctgctgc ttcgcaaacg
    24661 ccgacttata gccttgatgg tgcgacaccg ccaaacccgt gccgacaagc gcgataatgg
    24721 caattggttg ccagttattc gctagcagtt tcacgagatt cattctcgac ctcctgacgt
    24781 ttgacgctga ccaacgaacg cgccaccgca tagccgccga caatgcccaa atacaccgcc
    24841 caaatctccg ccgatgggtc gggcaacatc acaaatttaa acgtccccgc cgcgcaggcg
    24901 acgtttgccc acagcttcga gtgcgacaca ttgcctgtcg ccgggttttt aaaaatatcc
    24961 aaaatacgca ttgctattcc acacttttgg tttgcaggtg ccgtttcagc atttcctgat
    25021 aattggccag ttcgccctcc gcaaattcaa acgccgccaa gtctgcctgt tcgcttgcct
    25081 cacggctttt ggccgaccac agcccaatca tcttttcgta aaacgcaacc tgtcccatga
    25141 ttaacgacga ttcttgcgtt tgcgcgcagc ccgtttcgcg gctgccacgc ctgagtttct
    25201 taaatttggg cgtggatagg tgtgtatcgg acgaacattt ggcatctgta taagtgcgcg
    25261 gtttacctgc tcattactag gattttctcc aatcaatttt gtaagaaaat aggaagcagc
    25321 ttctttcaat ttaccaataa tattcatact cttgccgccc ccaatttcat cgcaatcgcg
    25381 tccgcaatcg cgcggcagat gccccatttg gtcgtcttaa acaaggccaa atcagtgtcg
    25441 ttgctgatga aaaaaggctc aacaatgccg ccagcctgcg cataagccag gcgcgaatga
    25501 tggcccgcat tgtcgggttt aaagccgtct tcgccgcgca gtttccagcc ggttttcttg
    25561 gcaacagctt tgctcaacac ctgacaccag cgtttgtttt taacggtact caacgcctca
    25621 atgcccgtag ctgctttacc ggccgccgag ttggtatgga actcaatcgc cacatccgag
    25681 ccgcgaatca gttttacagc ttcacgcagc ggcatattgc ctttgcctgt gccgtcggtt
    25741 ttaacagtca aaccgtagtc atcgcgcaaa atagcagcca caatgttgcg catatcctgc
    25801 gccaagtccg cctcacggtc gctgccgttg accgcacccg ggtcggtgtt gctgtgtcca
    25861 gcggttaagg ttacggtttt gcccattcat catctccaaa aaatattgat tgcatatagt
    25921 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacggaacc
    25981 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac
    26041 gccgtactgg tttttgttaa tccactataa catttgaaaa ccccattaaa ccgtctttaa
    26101 cccatcgtcc cgaacggtag atgtttcacc cctgcaaacc aaaaaaaaga ccgtctgaat
    26161 acagacggcc aaagcctggt cacacatcac tgcctaaaat aaagctgcct gctgtgccgc
    26221 aggtcggctc atttcattga taatcgtata tcccgttcgt gaagagatgc cgtatttagg
    26281 gcatagcttc gtcatcgcca taagcccgct ctccttatca atatcgcgca atttgacaaa
    26341 ctcctgataa aacctatggt ttctcaactg tattaacgcc ttgccgcacc gtgggacata
    26401 caattcctcg ccaccatata cctgcaacag ctcatgtgtt ttcacttcgc cgatggcttc
    26461 gaccaaaatt gccaaacgct cggtgtccac cttgccctta ccaaatttaa accgcgcccc
    26521 gccaatcgcc ttgaccagct gttccgtcgc tgccagtccg atgacatcca caatgtccaa
    26581 cacggtatcc ggcaataaat gttcaacttt ttcgaacccc atcatcccca cccgcttttt
    26641 ccgttttcct gttttccgca atctgcaacg cagcaaccag tttatgtagc tgcgtatcgt
    26701 ctaaatattc gaccttatcc ttaccaaaca tccgccgcgc cattgcgtgt gcatagttcc
    26761 aatgtttgcc gccgacggtc agcagggctt cgactttgtc caacattgcc gctgatgatg
    26821 tccgacgcag atgtggttta ccgtgtgggt taccttttgc tttaggctta aatccgtgcg
    26881 accgcatatc agcgacaaca gactcaagtt cggaaacatc catatccgca cacgaccgct
    26941 tgcccgtcac gcgctccaac accgcgcgat aggtaccgtc gtccaagccc agctcctttt
    27001 gggcaatttt aatttttgca atcaacgccc ggcgcatctc aaacccataa aacacaatat
    27061 attgactaat taacgcatat tataccaata aaatacaata tatagtatta agccgatgtt
//
LOCUS       DAXIHS010000021        25999 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXIHS010000021 DAXIHS010000000
VERSION     DAXIHS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.021"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(140..700)
                     /locus_tag="ACFOF2_000769"
     CDS             complement(140..700)
                     /locus_tag="ACFOF2_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC7107122.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
     gene            829..1020
                     /locus_tag="ACFOF2_000770"
     CDS             829..1020
                     /locus_tag="ACFOF2_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107123.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            complement(1216..1410)
                     /locus_tag="ACFOF2_000771"
     CDS             complement(1216..1410)
                     /locus_tag="ACFOF2_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC7107124.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(1400..3481)
                     /locus_tag="ACFOF2_000772"
     CDS             complement(1400..3481)
                     /locus_tag="ACFOF2_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC7107125.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3957..4337
                     /locus_tag="ACFOF2_000773"
     CDS             3957..4337
                     /locus_tag="ACFOF2_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC7107126.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(4470..4541)
                     /locus_tag="ACFOF2_000774"
                     /pseudo
     CDS             complement(4470..4541)
                     /locus_tag="ACFOF2_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4992..6425
                     /locus_tag="ACFOF2_000775"
     CDS             4992..6425
                     /locus_tag="ACFOF2_000775"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC7107127.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGTLASKF
                     RNSGQTCVCTNRVYAQSGIYEEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            6534..7322
                     /locus_tag="ACFOF2_000776"
     CDS             6534..7322
                     /locus_tag="ACFOF2_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC7107128.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7690..9246
                     /locus_tag="ACFOF2_000777"
     CDS             7690..9246
                     /locus_tag="ACFOF2_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC7107129.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            9263..10009
                     /gene="surE"
                     /locus_tag="ACFOF2_000778"
     CDS             9263..10009
                     /gene="surE"
                     /locus_tag="ACFOF2_000778"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC7107130.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
     gene            10265..11512
                     /locus_tag="ACFOF2_000779"
     CDS             10265..11512
                     /locus_tag="ACFOF2_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC7107131.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(11719..12201)
                     /locus_tag="ACFOF2_000780"
     CDS             complement(11719..12201)
                     /locus_tag="ACFOF2_000780"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC7107132.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(12354..12515)
                     /locus_tag="ACFOF2_000781"
     CDS             complement(12354..12515)
                     /locus_tag="ACFOF2_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107133.1"
                     /translation="MSEQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(12589..13050)
                     /gene="recX"
                     /locus_tag="ACFOF2_000782"
     CDS             complement(12589..13050)
                     /gene="recX"
                     /locus_tag="ACFOF2_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC7107134.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(13107..13814)
                     /locus_tag="ACFOF2_000783"
     CDS             complement(13107..13814)
                     /locus_tag="ACFOF2_000783"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC7107135.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            14221..15486
                     /locus_tag="ACFOF2_000784"
     CDS             14221..15486
                     /locus_tag="ACFOF2_000784"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC7107136.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(15544..16350)
                     /locus_tag="ACFOF2_000785"
     CDS             complement(15544..16350)
                     /locus_tag="ACFOF2_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC7107137.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            16695..16904
                     /locus_tag="ACFOF2_000786"
     CDS             16695..16904
                     /locus_tag="ACFOF2_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC7107138.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLAHVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            16993..18207
                     /locus_tag="ACFOF2_000787"
     CDS             16993..18207
                     /locus_tag="ACFOF2_000787"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC7107139.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(18294..20873)
                     /gene="clpB"
                     /locus_tag="ACFOF2_000788"
     CDS             complement(18294..20873)
                     /gene="clpB"
                     /locus_tag="ACFOF2_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC7107140.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            21134..22144
                     /gene="trpS"
                     /locus_tag="ACFOF2_000789"
     CDS             21134..22144
                     /gene="trpS"
                     /locus_tag="ACFOF2_000789"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC7107141.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            22150..22491
                     /locus_tag="ACFOF2_000790"
     CDS             22150..22491
                     /locus_tag="ACFOF2_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107142.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            22810..23154
                     /locus_tag="ACFOF2_000791"
     CDS             22810..23154
                     /locus_tag="ACFOF2_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107143.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAAKDTLNKAADAT
                     QEAADKMKDAAK"
     gene            complement(23363..24871)
                     /gene="ppx"
                     /locus_tag="ACFOF2_000792"
     CDS             complement(23363..24871)
                     /gene="ppx"
                     /locus_tag="ACFOF2_000792"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC7107144.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24988..25719)
                     /locus_tag="ACFOF2_000793"
     CDS             complement(24988..25719)
                     /locus_tag="ACFOF2_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC7107145.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSRRLSERWRLTLNVGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTLVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         5957 a         6490 c         7211 g         6341 t
ORIGIN      
        1 tctaaggtgc tgaagcaccg agtgaatcgg tttcgtacta tttgtactgt ctgcggcttc
       61 gtcgccttgt cctgattttt gttaatccac tataaaattc cggattccca cttttgtgga
      121 aatgatgaga taaacgtgat tatggtttaa acggggcgta tgcccattcc ccgggtttta
      181 aatccaaatc aaacagtttc agtctgccgc ttgccactct gatcagacgc aggcagggat
      241 agcccgcctt ggcggtcatt cgcctgactt ggcggttttt gccctcagaa atggtaattt
      301 caatccaaaa atcgggaacg gttttgcgga cgcgtatcgg cgggatgcgc tcccataacg
      361 aatctgcttc tccgtgtttc aagatgcgga tgcttgccgg acgggtaacg aaaccgccta
      421 agtctatccc ttttcttagg ctttccaatc ggctttcgtc gggtacgccc tccagttgcg
      481 cccagtaggt tttagggtgt ttgaatttgg ggtcggtaat ttgtgcctga agcctgccgt
      541 cgtcggtcag cagcagcagc ccctcgctgt cggtgtcgag ccgtccggcg gggtagaagc
      601 cgggaaaatt gataaagtct ttgaggcttt tgtgtttttc gtgcggtgaa aattggcaga
      661 taacgccata gggtttgttg aaggcgatga ggtttttcat agcttttggg tttgggttga
      721 aatgggcggc ggtatcggga aaatttgttt attgcgaagg gacatccgat aaatggtgct
      781 tccaaagtga aaaggtttga atgcagatta aattttaagg tgcatgaaat ggattttcaa
      841 ttctttgtcg aaacaatccg ccgacaggaa aagaaactga agcatggcgt gtaccggctg
      901 tttgagattt acagttggtt ggaagatgga aaagcaacaa aggcgcgtat ccggtatttt
      961 gtcaaaattg aggtcgataa acagatattg cgaaggattc atcgtggtat agcggattaa
     1021 caaaaaccgg tacgtcgttg tctcgcctta gctcaaagag aacgattctc taaggtgctg
     1081 aagcaccaag tgaatcggtt tcgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     1141 tgatttttgt taatccacta tattataaaa attccgtctg aagcggcgtt cagacggaat
     1201 ttgtttcaag caggcttaac agcagcgtcc gccgtttgcg ccgcagcggc gtttgcggca
     1261 atagtcttga aactgcagct tggtcatcac gggggcgttg ggattatgtt tgcgctgctg
     1321 tgcaacgtag ttttcataat cgggcacgcc tgccatcaag tttgccgtca gcttgatggt
     1381 tttccaccaa gacgcgagct tatgcttcac tttgtgcctc cggctgtttg ccgtcgcggt
     1441 acaccgccgg gatttctttg gcggtcggcc agccgacttt gcgtgctttg agggcggtac
     1501 gcaaaccgta cgcggcgaca atcacgacaa ccgacaagaa gaggatggtc agaccggcat
     1561 taatcttgtc gttgaagatg atttgcgcca tttcgccgat gtctttggca ggcgcaagga
     1621 cttcgttttt agccaatgcg tcgctgtatt tgccggcgtg ggcaaggaag ctgatgcgcg
     1681 ggtcgctgtg gaacagtttt tgcaggccgg cgtagcaggt tacgaacagt acgccgacgg
     1741 cgggaacgag taccacccag acataacggt cgcgtttcat cttaatcagc accacggcgc
     1801 acataatcaa ggctacgcct gccagcattt ggttggcgat gccgaacaaa ggccagagcg
     1861 agttgatgcc gcccagcggg tcggttacgc cggtgtagag aaagtagccc cacaatgcca
     1921 cggcgaagaa ggtcgcaatc aggttggcgg ggatggagtc ggtgttgccg aaaggtttgt
     1981 agaagatgct gcccaagtct tgaatcataa aacgtgcgac gcgcgtaccg gcatcgacgg
     2041 cggtcaggat gaacaaggct tcaaacaata gggcgaagtg ataccagaac gccatcatcg
     2101 cctcgcccgg aatcaggcgg ctcataatgt gcgccatacc gactgcgagg gtgggcgcac
     2161 cgcctgcacg ggaaaggatg gtgttttcgc ccacttcctt agcggtgtgc aacagggttg
     2221 cagcatcgac agggaattgc agcttggtgg taatcacttc ggcggcggta ttggcatccg
     2281 taccgatcag ggcggcaggg ctgttcatgg cgaagtacac gccgggatca agcgatgcgg
     2341 cggcggcaag tgccataatg gctacgaaac tttccatcaa cataccgccg taaccaatca
     2401 tacggacgtg ggtttcgttt tccagcattt tcggcgtggt gccggaggaa atcagcgcgt
     2461 ggaagcccga aaccgcaccg caggcgatgg tgatgaagag gaacgggaac aatgcgcctg
     2521 agaataccgg gccggaaccg tcgataaagt gggttacggc aggcatttgc aaagtgggat
     2581 tgacgatgac gatgcccaaa gccaaggccg caatcgtacc gattttcagg aaggtggaga
     2641 gatagtcgcg cggagtgagc agcagccata cgggcaatac ggaggcgaca aagccgtaaa
     2701 tcataatcgc ccaagtgagc tggatgccgt caaggtcgaa ccaatgtccg atggaacttt
     2761 tcgccacatc ttcgccgtaa attaccgcca gcatcagcaa aataaagccg acgatggaaa
     2821 tctcgccgat tttgcccgga cggatatagc gcgtgtaaat acccataaac agggcaatcg
     2881 gcatagtggc ggcgatggta aacgtacccc aagggctgtg aaccagtgct tttacgacaa
     2941 tcaacgccaa caccgccata atgatgacca taatcatcaa aataccgatg gaggcaatca
     3001 cgccggggac agtgccgagt tcctgtttca caatatcgcc caaagactta ccgtcgcggc
     3061 gcatagagac gaacaagacc atcatatcct gtaccgcgcc ggcaaacacc acgccgaaga
     3121 taatccacaa agtaccgggc aggtaaccca tttgcgccgc caaaaccggg ccgaccaaag
     3181 ggcccgcgcc cgcaatcgcg gcaaagtggt gtccgaacaa tacgcctttg tgcgtcggaa
     3241 cgtagtccaa gccgtcgttg tggcgttctg ccggagtcag gcggttagga tcgagccgca
     3301 ttacgcggtt ggcgatgtag aggctgtaaa aacggtaggc gatgcagtaa acggatatgg
     3361 cggcggtgac catccatacc gcgctgacct gttcgcctcg gctgagggcc agagtggtaa
     3421 aggatgctaa gccgaccagt accactatgc cccaaatgag gaaggttttg agtgatttca
     3481 tcgaataaat ccttatctca cactgtcgga atatgcctga acggcgggaa acggatgacc
     3541 gcctgccggc acgggcacaa accggagtct gaatgattga cgaagtatga aacagtgcgc
     3601 ttgtcgggat ttgtgcctta tgcccgcatc aaacagtgca gggatgcggg cgcacaaaac
     3661 gttaaacgcc gaataggatt ttaacgcaaa ttagcacact gatagcggtt tttacttgga
     3721 atttggaaaa aatttacatt ccttcgggcg gacaggcggg tttggacggc atcgtcaggc
     3781 aaaaggcggt gccggaagac gggtaaagaa ggggcgcgca atccggatta ttgattcatc
     3841 gcgggtaaat tccggttatc gggcttgtgt gtttgcgcgt ccgtttatag tatggcgttg
     3901 ccgcagcttg gaatcagggc ggttgtttca tatcttattt ttattgggga gcttttatga
     3961 atatcaggtt tttcgcgctg accgtaccgg ttttgtcttt ggcggcctgt gccgtgccgg
     4021 aagcgtatga tggcggcgga cgcgggcata tgccgcctgt tcaaaaccaa gccggcacgg
     4081 acgattttcg ggcgttttcc tgcgagaacg gtttgtctgt gcacgtccgc cgtttggacg
     4141 gcggcagaat cgcgttgcgg ttggacggca ggcgtgccgt cctctcttcc gacgttgccg
     4201 catccggcga acgctatacc gccgaacacg gtttgttcgg aaacgcaacc gagtggcacc
     4261 agaaaggcgg cgaagccttt ttcggcttta ccgatgccta cggcaattcg gtcgaaactt
     4321 cctgccgcgc ccgctaaacg gggcagcctg tttgaaccgc cgtccggatt tttcgggcgg
     4381 ttttttgtgt cggatttgtt ttggctgtcg gctggtttcg gcggttttcg ggcgtggcgg
     4441 attatttttc gtttcgttcc gggtttatcc tatcggcagc ggctcaatgc caactttaaa
     4501 cctgctccga tttcttcagg gctgttatcc aatgataaaa tatagtggat taacaaaaat
     4561 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt cacttggtgc
     4621 ttaagcacct tagagaatcg ttctctttga gctaaggcaa ggcaacgctg tactggtttt
     4681 tgttaatcca ctataatgtt tggatttttg ccatactcta ccacacgttg caactgcaat
     4741 ctttgttcct tatatattat taaagggctg cattactttt ctttctaaat taccattgta
     4801 taaaaagtat ttttcttaaa cgacagggat tgcgcccgct ccatattcag acaccgcccg
     4861 gatgttgcgc tgcccgatcg gatgcttcag acggcatcgg aagggtttgc agttttggaa
     4921 tatgaagatg ataatgtccg cgagattgac ggcatttgaa agcggttatt ccgcataccg
     4981 gaggatacga aatgaacgaa tattcccaat taatcaagca tcccgatatt tcccttcccc
     5041 cggtttcaga cggcatcaaa gtcgataatc cggcaacggg cgagactttg gcgtttgtcc
     5101 gcaagacgga ttcggacaag ctgaaaaacc tgattcaaaa ggcggcggca gcgcaaaaat
     5161 tatgggcggc aaaaaccgcg ttggaacgcg ccgatgtgtt gtggcgttgg tattttttga
     5221 taaaggaaaa caaagaagca ctggcgcgtc tcatgacgat ggagcagggc aaaggcctga
     5281 ccgaggcgcg cggcgaaatc gattatgcgg cttcgtttgt gcgctggttt gccgaagagg
     5341 cgcggcggat tgacggcgat gtgctgacga gtgtgaaagc gtcgcaaaaa ctggtcgtgt
     5401 tgaaacagcc tgtcggtgtt accgccgcga ttacgccgtg gaactttccg tccgcgatga
     5461 ttgcgcgcaa ggctgcgcca gctttggcag tgggttgcgc gatgatagtc aaacccgcgt
     5521 cgctcacgcc tttgagcgcg tatgcgttgg ccgtgttggc ttacgaagcg ggcgtgccgc
     5581 aggatttgct gcctgttgtc agcggcagtg cttcggaaat cggccgtgaa tttgccacaa
     5641 atccgattat ccgcaaaatc agtttcaccg gctctaccga agtcggcgcg aaaatttttg
     5701 ccgacagcgc ggcagacatt aaaaaactca gcttggaact gggcggcaac gcgccgttta
     5761 tcgtgtttga cgatgccgat ttggacaaag ctgtcgaagg tacacttgcc agcaagttcc
     5821 gtaatagcgg tcagacctgc gtctgcacca accgcgttta cgctcaatcc ggcatttacg
     5881 aggaattttg ccgcaaattg agtgaaaaag cagccgcgct caaattgggc aacggcttgg
     5941 aggatggcgt aaaccaaggg ccgctgattg aggaaaaagc ggtagaaaaa gtcgagcagc
     6001 acatcgccga cgcgctctca aaaggcgcgg tctgtctgac cggcggcaaa cgcagcgcgt
     6061 tgggcggaac gttttttgag ccgaccgtct taagcggtgt aacggcgcaa atggcggtag
     6121 ctcgcgagga aactttcggg cctttgtgtc cggtattccg ttttgaaacc gaagccgagg
     6181 tcatcgaggc tgcgaacaat acggaatacg gtttggcagc ttaccttttc accgccgaca
     6241 ccgcccgcca atggcgcgtc ggcgaagcct tggaatacgg catggtcggc atcaatacgg
     6301 gcttaatcag caatgaagcg gcaccgttcg gcggcgtgaa acgcagcggt ttgggacgtg
     6361 aaggcagcaa atacggtgcg gacgaatatc tagaattgaa atatttgtgt atggatgtcg
     6421 ggtgagggat gccatccgaa cggcagttcg ccggactgtg ccgcatttta agcagtctct
     6481 atctgttgta caatgcgccc tgtttttacg gttatttttg atttgaacaa gatatgatgg
     6541 aaaacggaaa aacagtcccg agatggcgtt ttgccttgaa aagtgcgggc tggcacctct
     6601 taatcagcct atcggttgca ggactggcgg cattgctggt ttttaaggtt tggtatcctt
     6661 atccttatgc cgagctgacg ggagggctgt cgctttatca gctggtggtg gctgtcgata
     6721 tcgtatgcgg cccgctgctg actttaattt tggcaagccc gaagaaaaag acaaaggcac
     6781 gcatggtcga tttttccatg gtcggcatca tccagctggc ggctttggcg tacggtctgc
     6841 acagcgtttc gctggcgcgt cctgtggtgg aagcgtttga acaggatcgt atgaccattg
     6901 ttacggcggc ggaagtcgtg gtcgaagatt tgcacaaagc ccccgaaggg ctgcaaagcc
     6961 tgtcgtggtt cggcatccgc cgcattgcat tgaaagaacc tgaggatgcg gatgagaaga
     7021 acaagacgct ggatttgtcc ctgaaaggta tcgagccgag tatgcgtccc gaccggtggc
     7081 tgccgtattc cgacaaggaa gcagaagaaa tccgcaaaca tctgaaaccg ctgaaagtct
     7141 tggcggatgc gagaaaaacg acggttgcgg acattctgaa acaggcaggt ctcgccgaag
     7201 gggaggagct gtattacctg ccgtttacca gcagcaggca gaaagagtgg atagtcatta
     7261 ccgataaaga gggcaacacc aaaggctacg cgccgataga cggcttcatc atcacccctt
     7321 aagcgttggg actccgtccg cactcgaaca tccgttcttc gcggcggtag aatcagactg
     7381 tatttgagag aggcgcaacc cctcaatcag ggcaacacat aggggaaggc tttatgtgcc
     7441 gtccgtgtgt tgaaacattt caaaataagc tcatgccgtc cgaacatcct ttcagacggt
     7501 atggcgtttt gccattgccc cgataagctg ttaaactatt taaattattt cgaccgaagg
     7561 tacacgcaca tgcaagaccc cgagcagagc agcaagcccg cccgccgttt tttgtgcgta
     7621 ccgtctgttc aggcagcttg agcgcttcag gctgcctttt cgcgtttgaa cttgaggaat
     7681 acgaaaatca tggatttcag ttggttggca gaaccgcata cctggatagg ttttgccacg
     7741 cttttggtgt tggaagtcgt attggggata gacaatcttg tctttgtggc gattttggca
     7801 aacaaggtcc agcccgcacg gcgcgaccgc gcacggatta tcgggctggg gctggcagtc
     7861 gtcatccgca tcattatgct tgcttttatg gcgcacatca tcacgctgac cgagccgctg
     7921 ttccaaatcg gcggcctcgc cgtttccggc aaggacatga ttatgctcgc gggcggtatt
     7981 ttcctgcttt acaaagccac caccgaactg catgaacgcc tcgaagggca caaccgtttt
     8041 accgttgccg acagccaaaa aaaacacgcg ccgttttggg gcgtggtcgc gcaaatcctg
     8101 atactggatg ccgtgttttc catcgattcg gtcattactg cggtggcgat ggtcgatcat
     8161 atcgtcgtgg cgatgggtgc ggtcgtcgtc gcaatggctg taatgatttc tgccagcaaa
     8221 ctcttgaccg aatttgtcga cagacaccct accgtcgtga tgctctgcct tggttttttg
     8281 ttgatgatcg gtttcagcct gattgccgaa gccttccatt tccacattcc caaaggctac
     8341 ctctacgccg ccatcggctt ctcgatttta atcgaactgt ttaaccagat ttcccagcgc
     8401 aacagccgca aaaacgacta catcggcagc tcgtggcgca aacggactgc tgaaaatgtc
     8461 ttgggtatga tgggcatacg cgaaagcgtg cttgccgatg cgggcggcga atctggggac
     8521 gacgcgcatt ttgaagaaaa cgaaaaatcg atgatacgca gtgtgctgac gcttgccgaa
     8581 cgcccgatta tgggggtgat gatcccacgc cgcgacatcg aacggctgga catttcccaa
     8641 agccgcgaag aacagtatgc ccaactgcaa aacacgcctt acagccgcct gctcgttgtc
     8701 ggaaaggcgg gcgtggacga acctttgggc tacatcaaca aaaaagacct gctgtcccaa
     8761 ctgctggaaa caggcggtct cgacattcag acggcattgc gccagccgct cgttttgccc
     8821 gacagcacta ccgcgctggg cgcaatcgaa ctcttccgcc aaagcagcgc ggattatgct
     8881 ttggtggtgg acgagttcgg cgcggtattg ggcatggtaa ccatgaaaga cctgctcgaa
     8941 accatcgcag gcgagttccc cgaagaattt gagcgcgaag aagaaccagc cgttcagggg
     9001 aatcccgatg aaagcctgac ggtggaaggc gcgttggaat atgtggaact cgcaccgcaa
     9061 ctcaacctgc cgcagcagca ggaagatgcc gatttccata cggttgccgg gctgattatg
     9121 gaagaattgc aaaccatccc cgatgtcggc gattttgccg atttccacgg ctggcggttt
     9181 gaagtggtcg aaaaagaagg gcagcgcatc gagcgggtca aaatcaccaa attgcccgaa
     9241 gaataagcat tcaggataga aaatgaacgt tttgatttcc aacgacgacg gctacctctc
     9301 cgaaggtatt gccgttttgg cgcgcgttac ggcggaattt gccaacgtca gggtggttgc
     9361 gcccgaacgc gacaggagcg gggtcagcaa ttcgctgacg ctggaacgcc ctttgcagtt
     9421 gaaacaggcg caaaacgggt tctactatgt caacggcacg ccgaccgact gtatccacat
     9481 cgggcagtct gtattttcgg attttcaggc cgattttgtc ttttcgggca tcaaccgggg
     9541 cgcgaatatg ggggacgaca cgctttattc ggggacggtt gcggcggcaa ccgaagccta
     9601 ccttatgggc atacccgctg tggcgttttc cttaaacgac gcttccggac gctattgggc
     9661 gaccgcagaa caggcactgt ggacattgtt ggcgcatttt ttcaaaaacc ccccgcagtc
     9721 ccctattttg tggaacatca atatccccgc cgtcgagccg gaagatgtgc ggggcattaa
     9781 aatcgcccgt ttgggcagga ggcatcacgg tcagaacgtc atccccgcgc gcaatccgcg
     9841 cggcgaacag atttattgga taggcccggt aggcagggtt tccgacaaag aagagggaac
     9901 ggatttcggt gaatgcggcg caggtttcat taccgtaacg ccgctgcaaa tcgacctgac
     9961 cgcctatccg gacatggcgg aaacagcggt gttctggcat gcggactgac cgtttcatca
    10021 aatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga
    10081 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctgcggc
    10141 ttcgtcgcct tgtcctgatt tttgttaatc cactataccg tcgggctttg cgcttgagaa
    10201 gaacgcgcat ttttattatg atataccgca ttcaggctat atatcggaac atgaaggtta
    10261 tcttatgttg aaacaaacga cacttttggc ggcttgcgcc tcctttgccg cactgttggg
    10321 cggttgtgct acccagcagc ctgcccccgt cattgcaggc aattcaggta tgcaggacgc
    10381 gccgtcttcg gcagtttaca acaaccccta tggagcaacg ccgtacagcc cggctcctgc
    10441 cggcgatgcg ccttatgtgc cgccggtgca aagcgcgccg gtttatacgc ctcctgctta
    10501 tgttccgccg tctgcacctg ccgtttcggg tacatacgtt ccttcttacg cacccgtcga
    10561 catcaacgcg gcgacgcata ctattgtgcg cggcgacacg gtgtacaaca tttccaaacg
    10621 ctaccatatc tctcaagacg attttcgtgc gtggaacggc atgaccgaca atacgttgag
    10681 catcggtcag attgttaaag tcaaaccggc aggatatgcc gcaccgaaaa ccgcagccgt
    10741 aaaaagcagg cccgccgtac cggctgccgc gcaaccgccc gtacagtccg cacccgtcga
    10801 cattaacgcg gcgacgcata ctattgtgcg cggcgacacg gtgtacaaca tttccaaacg
    10861 ctaccatatc tctcaagacg atttccgtgc gtggaacggc atgaccgaca atatgttgag
    10921 catcggtcag attgttaaag tcaaaccggc aggatatgcc gcaccgaaaa ccgcagccgt
    10981 agaaagcagg cccgccgtac cggctgccgt gcaaacccct gtgaaacccg ccgcgcaacc
    11041 gcctgtgcag tccgcgccgc aacctgccgc gcccgctgcg gaaaataaag cggttcccgc
    11101 gcccgccccg caatctcctg ccgcttcgcc ttccggaacg cgttcggtcg gcggcattgt
    11161 ttggcagcgt ccgacgcaag gtaaagtggt tgccgatttc ggcggcaaca acaagggtgt
    11221 cgatattgcc ggtaatgcgg gacagcccgt tttggcggcg gctgacggca aagtggttta
    11281 tgcaggttcc ggtttgaggg gatacggcaa tctggtcatc atccagcata attcttcctt
    11341 cctgaccgca tacgggcaca accaaaaatt gctggtcggc gaaggccagc aggtcaaacg
    11401 cgggcagcag gtcgctttga tgggcaatac cgacgcttcc agaacgcagc ttcatttcga
    11461 ggtgcggcaa aacggcaaac cggttaaccc gaacagctat atcgcgttct gactttctgc
    11521 ctgaaatcaa gttggaaatg ccgtctgaaa ggttttcaga cggcattttt gtttttggcg
    11581 gctgtgtttt caaatcattc ggaagggacg gggcatataa atttgtttga ctgtgtttgg
    11641 gtatttgctt gatttcatcc gtttgcagta gaaacggatg gcgcgatgtg cctgtctttc
    11701 agacggcata gtccgccgtc agcagccgca ggacatgtct tcagaggctt gcaggctgat
    11761 gtctctgcgt tttttggctt tttcgtagcg gcagcgttgg cggtcggtca ggatttccag
    11821 cggagggaca gggacgggtt tgccgtcttt gaccgcaacc atcgtgaagt agcagctgtt
    11881 ggtgtggcgg atttcgccgg tgcggatgtt ttgtgcttcg acacggatgc cgatttccat
    11941 cgaggtgcgt cccgtgtagt tgacagcggc gtaaaaggta accaaatcgc cgatgtggat
    12001 gggttcttta aacaggactt tgtcaaccga cagggtaacg caataattgc cgctgtaacg
    12061 gctggcgcag gaataggcaa cctggtcgag caggagcagg agttcgccgc cgtgtacgtt
    12121 gccgctgaaa ttggcggtgt ccggcatcat cagttcggac ataatgagtt cgtgggaagg
    12181 cagttggcgt tgttgtgtca tagtgtacct gttttaagaa agggtatttt gaaatgggtg
    12241 tggaaaaatt ttagggaatg ggaaagtgga attcaagcta aaaaaagcta tgatgggata
    12301 gcctgccgaa atgggctgcc gatgccgtct gaaggtttca gacggcattt tgtttaggaa
    12361 agccaaaaac ggaagaccag gaaaaacacg agtgccaata cgcccgccca aacgacgagc
    12421 atgaatccgg caacagcccg tcccatacgg taaaccgggt ttttgcgttc gtcttcgatg
    12481 ggggaggtgt ggtgtttttc gggttgttcg gacattttgt tctcaatcgg aaaagttgga
    12541 aacgggcgta aggttatgtt gccgtgcaaa aagattcaag aaggaaggtc agcagccgtc
    12601 ttcccaattt tcgtcccaag cgtgtttcaa tgccgtctga acggtatcgg catcgaatcc
    12661 gcgataggcg aggaaacgcg cctgtttttg tttttcttta aggtcggcgg cggggtgttt
    12721 gaatttttta cgcaacacgt ctatggcggc ctgtttttcg cttgagcggt cgggaagcag
    12781 gttgcggctg gtttcttcat ctatgccctg ttgcgccaaa gcctgtttca gcctcaatga
    12841 accgtgtttg cggcttttgc tgcggatata ggcttcggca tagcgcaaat ccgactgcca
    12901 gttgcgttcg gcaaattcgt ttaacacgtt ttccaactct tcttcgcttt cggcgtgcgg
    12961 tgcaagtttg cgtttcagac cgatgcggct gacttcttgg cgcgagagga tgtccatcgc
    13021 gcgggcgcgt agggattttt gcggtttcat ggcggcagtt tcggcgtgcg gcaggtttca
    13081 gacggcatag cgtgccgtcc gaatgcctac tcttctttat ttttctgcgg ttgcaggttt
    13141 tcgtaaattt cgggcagcga gccgataatc cagtcggggc gggtcgcatc gtcttgcgag
    13201 agcagcgtca tatcgccgta accgaaggta acgccgacgc tcaggcagcc ggcggctttg
    13261 gcggcgatga tgtcgttgcg cgagtcgccg accataacca tgtttgcaac atcgataccc
    13321 aaaacttcgg cggcgtgccg cagcggcagg gggctgggtt ttttttcggg caggctgtcg
    13381 ccgccgagta tcaggctgaa gtagtcggca agtccgagct gtttcaacag ttcggcggca
    13441 aggatttcgt ttttattggt aatgactgcc agcgggatgc ccaaagattt gagcaatgcc
    13501 agcccggctt cggtttcggg ataggggcgg gtgaagacgc tcaaatggtc gcggtagtat
    13561 ttcatatagg atacgaaacc tttttcccac agttcggaat cggcttcgcg gtcgcggtcg
    13621 ttggagagga cgcggtgaac cagtttgccg atgccgtcgc ccacatagct ttcgaccact
    13681 ttggcgggca gcggtttcat accgagttgt tccaacatcg cttctgcggc ggcggcaagg
    13741 tcggggacgg aatcgcacag tgtgccgtcc aaatcgaagg cgacggcttg gacgtgttcg
    13801 atggctgcat tcataatctg atttcctgtc gggttgaaag ggtttatttt aacatcgttt
    13861 gcacagggct tctaatgtat agattgttaa caatcgcaac aggttttgca ggcggtgttt
    13921 tcggatttcg gggcggaatc gggcaagggt ttgaaatccc ggcgatgttc caattaaatt
    13981 tttaaaaaat aaaaggatgc ctatatcttt atttaatttg acatttattt ttcggatgcg
    14041 cgcgctgctt tttcaggcgg agtccgggct gattccgtgc ggcttgaaaa ataaaatcat
    14101 atatagatta atcaaatcgg gcttatgccg actacacgct attacatttt gtcgggaaat
    14161 tggttatagt aatgagcgaa atgcacgccc ctgaaaatct ttttgtgaaa aggaagcaaa
    14221 atgtctgaag cccttgcaaa agaaaccctc aatccgtttg aaatcgcgcg caaacaggtc
    14281 aaaaccgcct gcgaccggct gaaaaccgat ccggccgttt atgaaatcct gaagagtccg
    14341 acccgcgtgt tggaagtcaa tttccccgtc aaactcgacg acgacacggt caaaaccttc
    14401 acgggctacc gttcgcaaca caacaacgcc gtcggcccct ataaaggcgg cgtgcgcttt
    14461 catcctggtg tgaatctgga cgaagtcaaa gccctgtcga tttggatgac catcaaatgc
    14521 tgcgttgcgg gtattcctta tggcggcggc aaaggcggcg ttaccttgga tccgcgcgat
    14581 tattccgagg cggaactgga acgcatcgcc cgcgcttatt ccgaagccat ttctccgctg
    14641 attggcgaga aaatcgatat tcccgcacct gatgtgaaca ccaacggcaa aatcatgtct
    14701 tggatggttg atgcctatga aaacgtggtg aaacattctg caccgggcgt atttaccggc
    14761 aaaccggttg agttcggcgg ctctttggcg cgtaccgaag ccaccggtta cggcgtgaat
    14821 ctcgctgccg tccaagcttt ggaaaaactg ggcaaagacg tgaagggtgc cacttacgcc
    14881 attcaaggtt tcggtaatgt cggctaccac acaggttatt acgcgcatca atccggtgcg
    14941 aaaatcgttg ccgtttctac tgttgacgtt gccatctaca atgaaaatgg cttggatatg
    15001 gaagcaatca tcaaagaatt ccaaaccaaa ggtttcatca ccaatgaagc cggctacggt
    15061 aaagaaatca gcaatgccga acttttggct ctggaagtgg acgtacttgc cccttgcgca
    15121 ctggaaaacc aactgacttc tgaaaatgcc ggtaaagtcc gcgcgacaat cgtggtcgaa
    15181 ggcgcaaatg gcccgactac tccagaggca gacgttatcc tgcgtcaaaa cggcgtattg
    15241 gtcgtgcctg atattctggc gaactgcggc ggcgtggtcg tttcctattt cgagtgggtg
    15301 caaaacctgc aaggctatta ttgggagttt gacgaagttc aagagaaaga aaccgtcgtc
    15361 ctgcgccgag cgttccgcga tatttggaac ttggctcaag aatacgatgt cgatttgcga
    15421 accgcgtctt atatgatgag tatccgccgt gttgaaaaag cgatgaaact gcgcggttgg
    15481 tattgatgtt tgaacaaaaa atgccgtctg aagccttcag acggcatttt tgtgcgggcg
    15541 ggattaatgg tgcgaatggc cgatttggaa ggttaaagtc gagtagttcg cctgtttttg
    15601 gcacacgctt tgatcgggga agtcggcttt gtgttcgaca ttggctttcc agaagccttg
    15661 gcgcaagggg atgatgtcca cttcgccttt gtcgtctgtg ctgtcggaga aagcctgtgc
    15721 ttcggttttg tgcgttttgc tgcggtcgct ggtgtcgaag ccgtcaaagg tggcggtaac
    15781 ggtggcattg ggcagcggtt cgccacggaa cagaacgcgg actttgaagc gttcgcctac
    15841 gtgaatgttg gcgggattgt ccagcgggac gatttccaag ttttgtccga ccggtttggt
    15901 gatgatggcg gtgtccgcgc tttcgtgtcc gacgttgacg atgtttttgc cgaacatacg
    15961 ggtttgttcg caatagcttg cgtcaggcat ttctttgatg cccgcctgtt tccagcctgc
    16021 tttgtttttt gaccagaaag taggctgata ttcggcgatg acgaggtaac tgccgtcctt
    16081 aacgggacgg ttgcttcggt actggtagtt gtatgtgccg cgttgaatca tgttttcctt
    16141 gcctttttcg gtaaccagct gcatcggttt gctgaagatg tgcaggcggt ctttggcgat
    16201 gggttcgagt tcgggaaatt cgccgtagcc caagtcggct ttaaggtatt cgccgccgtg
    16261 cgtgtgggcg gtttcgaccc agacgcggtg ggcgtgggcg gatgtggcaa atagggcgga
    16321 aacggcgagc agtgtcaatg ttttcttcat ggtgcagcct ttgtctcgtg tgggttaaag
    16381 attttgttat gttataacaa aataggtatc gtgtgaagac ggatttgctg tctgaaaact
    16441 gtctttttgt atagtggatt aacaaaaacc agtacggcgt tggctcgcct tagctcaaag
    16501 agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtgcta tttgtactgt
    16561 cttcggcttc gccgccttgt cctgattttt gttaatccgc tatatcgatt tgaatttttc
    16621 agaaaatgaa gcggacgttt gggcggaggc aacttgtttg ataagatagc aatatttcaa
    16681 aacggagaaa gatcatgcct tatgtcaata ttaaagtaac cggcggcaag gaagcaccga
    16741 ctgccgcgca aaaagcggaa ctgatcggcg gcgtaaccga attgctggca cacgtgctgg
    16801 gtaaaaatcc cgaaacaacg gttgtcgtga ttgacgaagt ggataccgat aactggggaa
    16861 taggcggcaa aagcgtcagc gaacggcgca aagagggcag gtaaaaagcc tgaaaatctc
    16921 ggtttgatgc tttaaattcc gcgtgaaaaa gagtacattc ccacccattg cccaaaattt
    16981 acggaacaca tcatggataa atttcccaag tctgcaaagc tcgatcacgt ctgttacgac
    17041 atacgcgggc cggttcacaa aaaagccctt cagttggaag aggagggcaa taaaatcctt
    17101 aaactcaata tcggcaaccc tgcgccgttc ggctttgaag cccctgatga aatcttggtc
    17161 gatgtcatcc gcaacctgcc gacttcgcaa ggctattgcg attccaaagg gctgtattcc
    17221 gcccgcaaag ccattgttca ctactatcag accaaaggtt tgcgcgatat tacggttgat
    17281 gatgtctata tcggcaacgg cgtgtccgag ctgattacga tgtctatgca ggcattgctc
    17341 aacgacggcg acgaaatcct gattcccgcg cccgactacc ctttgtggac ggcggcggca
    17401 acgcttgcgg gcggtacggt acgccattat ctgtgcgacg aagaaaacgg ctggttcccc
    17461 aaccttgccg atatggaagc caaaatcacg cccaaaacta aagccatcgt cgtcatcaat
    17521 cccaataatc cgacaggcgc ggtgtacagc agggaaatcc tgttggaaat cgccgaactg
    17581 gcgcgcaagc acggtttgat tattttcgcc gacgagattt acgataaaat cctttatgac
    17641 ggcgcggttc accaccacat cgccgcgctc gcccccgacc ttttgacggt aacgttcaac
    17701 ggtttgtcca aagcctaccg tgtagccgga ttccgccaag gctggatggt gctcaacggg
    17761 ccgaaacatc atgcaaaagg ttacatcgag ggtttggata tgctctcgtc catgcgcttg
    17821 tgtgcgaata cgccgatgca gcatgcgatt cagacggcat tgggcggtta tcagagcatt
    17881 aacgaattca ttttgcccgg cggacggctt ttggaacagc gtaacagggc gtgggaattg
    17941 gtcaatcaga tacccggcgt atcctgcgtc aaaccgatgg gcgcgatgta tatgttccca
    18001 aaaatcgata ccgaaatgta ccgtatccac gatgacatga aattcgttta cgatttgctg
    18061 gtgcgcgaaa aagtcttgct ggtgcaggga acggggttta attggatcaa gcccgaccat
    18121 ttccgcattg tcacgctgcc ttacgtccat cagattgaag aggcgatggg caggctggca
    18181 agattcctgc aaacctaccg ccaataaggg gacggtttgt ctgccgagga taaaaaatgc
    18241 cgtctgaaac ggagattccc gtttcagacg gcattttcaa cagcaggaac gaatcaggca
    18301 aatttcagtc tgtcgccgtc ggcttctacc ctgatttcgc tttcgggcgc atagtttccg
    18361 gcaagcaggg cttttgccag cgggttttcg atttccgact ggatggcgcg tttgagcgga
    18421 cgtgcgccgt aaatcgggtc gaaaccggct ttggcgatga tgtccagtgc ggcatcggaa
    18481 acagtcaggc gcaggttttg tttttccaaa cgtttttcca aacctttgag ctggattttc
    18541 gcaatgctgc ggatgtttgc ctgatccagt ccgtggaaca cgactacttc gtcgatgcgg
    18601 ttgatcattt ctggacggaa atgttctttc acatcctcca tcacaacttc ttttaccgct
    18661 tcgtaatcct gaatgcccat ttgttggatg tgctggctgc cgatgttgga agtcatcaca
    18721 atcacggtat ttttaaaatc taccgtcctg ccttgcccgt cggtcaaacg accgtcatcc
    18781 aatacttgca gcaggatgtt gaacacgtcg ggatgggctt tttccacttc gtccaaaaga
    18841 atcacgctgt acggtttgcg gcggacttgt tcggtcaggt agccgccttc ttcgtagccg
    18901 acatagcccg gaggcgcgcc gatgaggcgg gcaacggcgt gtttttccat atattcggac
    18961 atatcgatac gaatcagatg atcttcactg tcaaacagga agcctgccaa tgccttacac
    19021 aactcggttt tacccacgcc ggtcgggcct aggaacagga agctgccgta gggtttattc
    19081 gggtcggcaa gaccggaacg gctgcggcgg atagcgtcgg acacggcacg cacggcttcg
    19141 tcttgaccga ctacgcggcg gtgcaacact tcttccattt tcagcaactt gtcgcgttcg
    19201 ccttccatca tttttgacac aggaatgccg gtcatacggg aaacaacttc tgcgatttcc
    19261 tctgcgccga cattattacg caagagtttg tttgccggtt ttgtgctgtc cgtatctgcc
    19321 cgttcggcgg ctgcacgctg tttttccaaa tgctccaaat cttcatacat caattttgaa
    19381 gccagtgcca aatcgccttg ccgttttgcc tgttcgattt taattttgac ttcgtcaatt
    19441 tgtttcttaa tattagcagc accgtctgaa atcgcttttt cggctttcca gatttcgtcc
    19501 aaatcggcgt attctttttc caaaccgtcg atttcctcat ctatcagttc caaacgtttt
    19561 ttgctggcat cgtctttttc tttttcaacg tgcgcctttt ccatccgaag ctgaatcaga
    19621 cggcggtcga ttttgtccat tgcttccggc ttggtttctt tttccatctt gacacggctg
    19681 gcggcttcgt caatcaaatc aatcgcttta tcaggcagga agcggtcggt aatgtagcgg
    19741 tcgctcaact ccgctgcggc aacgatagca gggtcggtaa tatcgatacc gtggtggatt
    19801 tcataacgct cctgtaaacc gcgcaagata gcgatggtgt cttctacact tggctcgcca
    19861 accaatactt tttggaagcg gcgttcgagt gccgcgtctt tttcgatgta ttggcggtat
    19921 tcgtccaagg tggtcgcgcc gatacagtgc aattcgccac gtgccaaagc cggtttcagc
    19981 atattgcccg catccatcgc accgtcggtt ttgcctgcgc cgaccaaagt atggatttcg
    20041 tcgatgaaaa tcagagtgtt gccgtcgtct ttcgccaagt cgttcaacac gcctttcaag
    20101 cgttcttcaa attcgccgcg gtatttcgcg ccggcaatca atgccgccaa gtccaaaacc
    20161 agtaggcgtt tgttgcgcag ggattcgggt acttcgccgt tgacgatgcg ttgtgccaaa
    20221 ccttcgacga tggcggtttt acccacgccc ggttcgccaa tcagcacagg gttgtttttg
    20281 gtacggcgtt gcaatacctg aatcgcacgg cggatttcgt cgtcacggcc gataacgggg
    20341 tcgagcttac cgtctcgggc gcgttgggtc agatcgagcg tatatttttt caaagcatcg
    20401 cgttggtctt cggcattggc atcgttcacg ttttgtcctc ctcgtaccgc gtcaatcgcg
    20461 gcattgatgt tttgttcggt cgcgccggct tctttcaaaa ttttgccggc cgcatcgttc
    20521 tgctgcacca aggcaagcag gaaaagttca ctggcgatat aggcatcgcc gcgtttggtt
    20581 gcggctttgt ccatcaggtt caacaccgcc tgcaattcgc ggctgggcag aatgtcgccg
    20641 ccctggccgg acactttcgg caggctgttc aaatgctgct gcaaacgctg tttcacctgc
    20701 ggcacgttca cgcccgcatg agccaagagc gcggcagcac cgctgttttg gtcgtcaagc
    20761 agggctttca gcacaaagcc cgcttccaga tagctgctgt ccgcagccaa cgccaaactc
    20821 tgagcttctg caagggcctg ttggaatttg gcggttaatt tgtcgtatcg catttttgtt
    20881 tccttttcaa aatgtccgct gtcgaagcct atatgtgcat aattgtggat aactcaagtt
    20941 ctgttttctg tttttctata tttaattcga tatatcattg aatttaaagt atataaaaat
    21001 gtataataat gtgtataact atatcttctt aatatggaaa agtctgttgt cggctggatg
    21061 taggtggcaa atcgggtata atcggcgcat ctttttccct ttcagacggc attgatgccg
    21121 caaggacatt tttatgagca aaaaacgagt tctgaccggc gtaaccacca ccggcatccc
    21181 gcatctgggc aactacgtcg gtgctatccg ccccgccgtc cgcgcggcgc aaaaccctga
    21241 taccgaatcc ttcctcttcc tcgccgatta ccacggtatc atcaaatgcc acgagccgga
    21301 gatgattcac caatccaccc aagccgttgc cgccacttgg cttgcctgcg gactcgaccc
    21361 cgagcgcacc accttctacc gccaaagcga cattcccgaa gtgatggaat tgaactggat
    21421 tctgacctgc atcaccgcca agggtttgat gaaccgcgcc cacgcctaca aagccgccgt
    21481 gcaggcaaat gcggaaaacg ggcaggaaga ccccgatttc ggcgtggaaa tgggtttgtt
    21541 cagttatccg attctgatga ctgccgatat tctgatgttc aacgccaacg aagtccccgt
    21601 cgggcgcgac caaatccaac acgtcgaaat ggcgcgcgac atcgccggcc gcttcaatca
    21661 ccgcttccga gaactcttca ccctgcccga agtgaaaatc gacgaaaacg tcgaactctt
    21721 ggtcggtttg gacggacgca aaatgtccaa atcctacggc aacaccattc cgctttggga
    21781 aaacgacaaa aaaacccaaa aatcggtcaa caaaatcatc accaatatga aagagccggg
    21841 cgagccgaaa cagcccgacg aaagcccatt gtttgaaatc tacaaagcct tctccacgcc
    21901 gtctgaaacg gtggaattta cgaaaatgct tgccgacggc ctggcttggg gcgaggcaaa
    21961 aaaacttttg gcggcgaaaa tcaacgccga actcgccgaa ccgcgcgaac gctacaacga
    22021 gctgaccgcc gacccttcgc aaatcgaaga gattttacag gcaggtgcgc aaaaagcgcg
    22081 taaagaagca cgcgaattgt tggacaaagt acgcgatgcg gtcggcatcc gcccgttgaa
    22141 ataaacccga tgccgtccga acctcttaaa ggcttgccgg aacaaaacgt cgtccggctg
    22201 accggcaagc atcccaacga cttggaagcc gtcagcggca aatgcatgga aaccgacgat
    22261 aagggcggtc cggtaggctg ggcggaaaac ggcgtgtgcc ataccttgtt tgccaaactg
    22321 gtgggcaata tcgccgaaga cggcggcaaa ctgacggatt acctgatttc gcattccgcc
    22381 ctgcaaccct atcaggcagg caaaagcggc tatgccgccg tgcagaacgg acgctatgtg
    22441 ctggaaatcg acagcgaagg ggcgttttat ttccgccgcc gccattattg aggtattcgg
    22501 acatcccgga atatatttgg gtttttcaaa ccttgcgaaa aaagtccgca ccgccgggaa
    22561 actcaaaagg agggggatat gtgttacaat tttccgaact gttttcataa aatagttttc
    22621 ggacgtgttt caatatggca ttgatgccgc cttattgttc gagaaaaaaa cctttatatt
    22681 taaatataat gggttttaac taaacgggaa accgttttct ctccggccga tgggcaaaat
    22741 cagccgattg atggaacacg gtccgatttt taagcaaaac cttaatcaac aactcaagaa
    22801 ggataagaca tgaaaaaatt attgattgcc gcaatgatgg cggctgcctt ggcagcttgt
    22861 tcgcaagaag ccaaacagga ggttaaggaa gcggttcaag ccgttgagtc cgatgttaaa
    22921 gacactgcgg cttctgccgc cgagtctgcc gcttctgccg tcgaagaagc gaaagaccaa
    22981 gtcaaagatg ctgcggctga tgcaaaggca agtgccgagg aagctgtaac tgaagccaaa
    23041 gaagctgtaa ctgaagccaa agaagctgta actgaagcag ctaaagatac tttgaacaaa
    23101 gctgccgacg cgactcagga agcggcagac aaaatgaaag acgctgccaa ataatttgtt
    23161 gctttggcaa aaatgacgag atgccgcctg ccggcaaccc aaacgaaacc gcctgaagat
    23221 tttcaggcgg tttttgagtt gtatgcccct tgttttttac gcctgcataa ccgttccgag
    23281 cagatgtgcc gcgcatatcg ggatgttgcg gacagggaaa tgccgtctga acatcattca
    23341 gacggcattt gccccgcaag gttcagacgg cctcgacttt gaacggcata ttgatttttt
    23401 gccattggac gctttcatat tccaatgcgt cggcaatcag gggatggcgt tccagccatt
    23461 ccctgtcaat acgcaggatg aagccgcaac tttccgtgtc cgtacgcaac tgcatatttt
    23521 tcgggaaaga caggtcttgg cgcgaacggc agaacagggc ggcaaggcgc agcgacaaaa
    23581 cggcatacca caacatttcg ttggtgccga tgatgccgct catttttttc atatcgccgc
    23641 gatgaccgat gaccagttgg gcaagtatgg tctgttcttt gcgtgagaaa cccggcatat
    23701 cggcgttttc gaggatgtag gcggaatgct tgtgatagcc ggtgtgggcg atgtccaaac
    23761 cgatttcgtg cagcgcggcg gcgcgtccga gatactgttg ccacaaggca agctcttgaa
    23821 ccgtaacatt tttagcgtgg cagaggctgt ccataaaggt ttgcgcggtc tcggcggtgc
    23881 gtttcgcctg attgaggctg acgtggtagc ggtgttggaa ctcggcaacc gtttgtccgc
    23941 gcatatcttc gtttaaaccg cgcccgatca aatcgtaaaa cacgccgtcg cgcagggcgg
    24001 cttcggttac ggtcatcctg tcgagtttca tttcttcaaa cgccgccatc atcacggcaa
    24061 gtccgccggc aaaaacttcg atgcgttccg gtttcaggtt ttcaaatttg gcctttttga
    24121 ccgaaccggc ttcgatgatg cgttcggcga gggcgcgcat gcctttgtag gtaatgtccg
    24181 cctcttgggg catttcggcg gcaagcacgt cgcggatgga ttttgccgaa cccgatgtgc
    24241 cgacggcgaa atcccaacct tcgcgcctca tatttttgct gatgcgctgg atttcgttgc
    24301 gggcggcgga aatggcagat tggaagtctt tggcggtgat tttgttttgg aagaagcgca
    24361 ggctgtaggt aacgcagccc aagggcaggc tttcggtaat gtcgggattc agcgtcgagc
    24421 cgatgacaaa ttctgtcgaa ccgccgccga tgtcgataac cagcattttg ccgccgccgg
    24481 gggggagggt gtggatcacg ccggtataaa tcagccgcgc ctcttcgcgc ccggcgatga
    24541 tttctatggg gaaacccagt gccgcttcgg ccttgggaag gaaatctgcg atgtttttgg
    24601 cgacgcggaa ggtgttggtt gccacggcgc gcacctgttc ggggcggaag ccgcgcaggc
    24661 gttcgccgaa ttttgccagg cagtccaaag cctgttcttg ggaagcggca ctcaggtttt
    24721 tctgttcgtc cagaccggcg gcgaagcgca ccatctgttt gaacgaatcg ataactttca
    24781 actgtccgtt gttgttttcg caaatctgga ggcggaaact gttggaaccc aaatcgacgg
    24841 aggcgaggat gtttgcgggg gtggtggtca tggcggatat cggtggggga aaaacgcaat
    24901 gttactctga cggcgcaggc gttgacaata aatgatgcgg ctgtttttga ttctgctcgc
    24961 ggatgttgcc gacggcattt tttgcgctta tttgaaatcc ttttccacgc tcatgaaaat
    25021 ctgcatgttt ttgcgtgtgt aaaaactttt catattgctg tcgattttca gatagcggaa
    25081 attgagttgc ggcgtaaagc ccttccaaga gattttgtcg tgccacaacg acaggtttgc
    25141 ctgatattcg tggtctttgc gcgggaagcg gtacacaagg gtcccgggtg catcaaaagt
    25201 cctgcgggtg tagcgcaggt ttgcccgcag acccaagccg ccgtcgaacg ttttgaccgc
    25261 tccgacacgc aaacccttgc ggatggaagc ctgttccgcc tccttcgtca agtcgtgcga
    25321 ccagtccgca ccgccgtaaa gcagccagtc tttcggcgcg gaatacatca gcgtcgcgcc
    25381 cgccagcggc atatggctgt cgtatcgggc ggcggtgcgg tcttcctgat aacgcttcca
    25441 catattgccc acgttcagcg tcagccgcca gcgttcgctc aagcgtcggg agaaatcggc
    25501 attgaagccg ccgacgaaat tgtatcggct gccgcctaag aggttttgcc cgacaaacgg
    25561 cacgatgccg aacgagcgcg ttaccgaacg atttttatag ccgagcgaca ggtgcaggca
    25621 ctgttcgctg aaatctttgt tatcccaata atgcacgccg ccgccgctga tgccgccgta
    25681 gaggaaatga tgcccgcccg cattgatttc gcgcgacacg cctaagccct agcgcaaacc
    25741 gtgtgccttt tgcggcaggc tgtcggcggt tttcgtccag cttctgcccg caaattcaat
    25801 cgtttttttg gacgaagcgt tgtttatagc ggattaacaa aaatcaggac aaggcgacga
    25861 agccgcagac agtacaaata gtacggaatc gattcacttg gtgcttcagc accttagaga
    25921 atcgttctct ttgagctaag gcgagacaac gccgtaccgg tttttgttca tccactataa
    25981 cagcaaccct gtcgccgtc
//
LOCUS       DAXIHS010000022        24375 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXIHS010000022 DAXIHS010000000
VERSION     DAXIHS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.022"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..735
                     /locus_tag="ACFOF2_000794"
     CDS             <1..735
                     /locus_tag="ACFOF2_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC7107146.1"
                     /translation="AAQAASEDSSRSPYYVQADLAYAAERITHDYPQATGANNTSTVS
                     DYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWNNNKYSVNTKEVLRNNNGNRKE
                     LKTENQENGTFHAVSSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVKKET
                     TTTFLAPTQGATVPGKIVEGPFSKPAYHESHSISSLGLGVIAGVGFDITPKLTLDTGY
                     RYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(709..918)
                     /locus_tag="ACFOF2_000795"
     CDS             complement(709..918)
                     /locus_tag="ACFOF2_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107147.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(936..1493)
                     /locus_tag="ACFOF2_000796"
     CDS             complement(936..1493)
                     /locus_tag="ACFOF2_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC7107148.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1569..3203)
                     /locus_tag="ACFOF2_000797"
     CDS             complement(1569..3203)
                     /locus_tag="ACFOF2_000797"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC7107149.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3481..4587)
                     /gene="serC"
                     /locus_tag="ACFOF2_000798"
     CDS             complement(3481..4587)
                     /gene="serC"
                     /locus_tag="ACFOF2_000798"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC7107150.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTEDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4952..5383
                     /gene="rimP"
                     /locus_tag="ACFOF2_000799"
     CDS             4952..5383
                     /gene="rimP"
                     /locus_tag="ACFOF2_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC7107151.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5411..6913
                     /gene="nusA"
                     /locus_tag="ACFOF2_000800"
     CDS             5411..6913
                     /gene="nusA"
                     /locus_tag="ACFOF2_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC7107152.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6926..9814
                     /gene="infB"
                     /locus_tag="ACFOF2_000801"
     CDS             6926..9814
                     /gene="infB"
                     /locus_tag="ACFOF2_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC7107153.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDTAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(10025..11419)
                     /locus_tag="ACFOF2_000802"
     CDS             complement(10025..11419)
                     /locus_tag="ACFOF2_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC7107154.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11493..12833)
                     /locus_tag="ACFOF2_000803"
     CDS             complement(11493..12833)
                     /locus_tag="ACFOF2_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC7107155.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12976..13602
                     /locus_tag="ACFOF2_000804"
     CDS             12976..13602
                     /locus_tag="ACFOF2_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC7107156.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13666..15084)
                     /locus_tag="ACFOF2_000805"
     CDS             complement(13666..15084)
                     /locus_tag="ACFOF2_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC7107157.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAVRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            15411..16139
                     /locus_tag="ACFOF2_000806"
     CDS             15411..16139
                     /locus_tag="ACFOF2_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC7107158.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16198..16686)
                     /locus_tag="ACFOF2_000807"
     CDS             complement(16198..16686)
                     /locus_tag="ACFOF2_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC7107159.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            16716..16928
                     /locus_tag="ACFOF2_000808"
     CDS             16716..16928
                     /locus_tag="ACFOF2_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC7107160.1"
                     /translation="MPILLDISRDGKGRIDTGSGADDFGFPRYAQNLTRDKTAICPHN
                     AHKKIHPEDLNFSKFSESKVYFNEII"
     gene            complement(17445..17909)
                     /locus_tag="ACFOF2_000809"
     CDS             complement(17445..17909)
                     /locus_tag="ACFOF2_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC7107161.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            complement(17919..18049)
                     /locus_tag="ACFOF2_000810"
                     /pseudo
     CDS             complement(17919..18049)
                     /locus_tag="ACFOF2_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18234..19292
                     /gene="alr"
                     /locus_tag="ACFOF2_000811"
     CDS             18234..19292
                     /gene="alr"
                     /locus_tag="ACFOF2_000811"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC7107162.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(19356..20711)
                     /locus_tag="ACFOF2_000812"
     CDS             complement(19356..20711)
                     /locus_tag="ACFOF2_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC7107163.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(20726..20998)
                     /locus_tag="ACFOF2_000813"
     CDS             complement(20726..20998)
                     /locus_tag="ACFOF2_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC7107164.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(21124..21861)
                     /locus_tag="ACFOF2_000814"
     CDS             complement(21124..21861)
                     /locus_tag="ACFOF2_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC7107165.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(22142..23041)
                     /gene="prmB"
                     /locus_tag="ACFOF2_000815"
     CDS             complement(22142..23041)
                     /gene="prmB"
                     /locus_tag="ACFOF2_000815"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC7107166.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            23186..23779
                     /locus_tag="ACFOF2_000816"
     CDS             23186..23779
                     /locus_tag="ACFOF2_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107167.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            23891..>24375
                     /locus_tag="ACFOF2_000817"
     CDS             23891..>24375
                     /locus_tag="ACFOF2_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC7107168.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     Y"
BASE COUNT         6482 a         6605 c         5992 g         5296 t
ORIGIN      
        1 gcagcgcagg cggcaagtga agacagcagc cgcagcccgt attatgtgca ggcggattta
       61 gcttatgccg ccgaacgcat tacccacgat tatccgcaag caaccggtgc aaacaacaca
      121 agcacagtaa gcgattattt cagaaacatc cgtgcgcatt ccatccaccc ccgggtgtcg
      181 gtcggctacg atttcggcgg ctggaggata gcggcagatt atgccagtta cagaaaatgg
      241 aacaacaata aatattccgt caacacaaaa gaggtgctaa gaaacaataa tggcaacagg
      301 aaagaactga agacggaaaa tcaggaaaac ggtacattcc acgcagtctc atcgctcggc
      361 ttatccgcca tttacgattt caaactcaac gataaattcg ataaattcaa accctatatc
      421 ggtgcgcgcg tcgcctacgg acacgttaaa catcaggttc attcagtgaa aaaagaaacc
      481 acgactactt tccttgcacc aacgcaaggc gctacagtgc caggcaagat cgtagaaggt
      541 ccgttcagca aacctgccta tcacgaaagc cacagcatca gcagcttggg tcttggtgtc
      601 atcgccggtg tcggtttcga catcacgccc aagctgactt tagacaccgg ataccgctac
      661 cacaactggg gacgcttgga aaacacccgc ttcaaaaccc acgaagtctc attgggcatg
      721 cgctaccact tctgattccc cgataccgat gccgtctgaa ccttcagacg gcatgagacc
      781 tttgcctgcg tacttggtgc gctggtcgcc tccgaacatg gcgcgacacc cgacatttcc
      841 cccgaacgca tcgagcgttt catgaatccg gtttaaaacg catggaaaaa tgccgtctga
      901 aagcctttca gacggcattt cgcttgagat tccgtttacc aatggctgac aaacgcttcc
      961 aaatcggtat tcttgggctt atgcacttcc tctgtcggcg tgccgaccat catcagcccg
     1021 atgattttat ccttatccgc acatccgaaa gcctcacgca acagggggct gttgacccac
     1081 atccccgtaa tccagacatt gtcgaaacct tgcgccgttg ccgccagttg cagcgcatac
     1141 gccgcacaac ccgccgtcag catctgctcc cattccggtt tcggcttagg cacatcgcgg
     1201 ttcggcgcaa acgttacacc aatgaccatc ggcgccatat tgcccacttt ttccgccttt
     1261 ttcatcgcat cgtcgccgaa attcaattcg gcaaccgttt gcttcaacac atcgcgaaaa
     1321 cgttgcaatc ctacctcgcc ttgaatcacg gtaaaacgga aggggcgcat attgccgtga
     1381 tcgggaactt gggttgccgc ctgaaatatt tgttccaact ccgccgcatc gggggcgggg
     1441 tgcttcagct ttttggaaga tcggcggttc gtcaataatt ttaaagcatc catatcgtta
     1501 tactccggtc atcggtcggg tgttcggaca catatgccgt ccgaaggctt cagacggcat
     1561 atccggcatc agcgcggacg gcggcaggct gccaatatat ccatttcctt ccgataggtt
     1621 tggctattgg aaatgtccat cagccccaat accgtgctga aatagtggtc gtgcgaatat
     1681 tcgttttccg ccgctttttg tttgaggcat tggaaatcta tgcccccgtg ttggcggaag
     1741 gctttggaaa accacataac catcgggata tgcgtctgcc cggaaggcgc gatggcgtaa
     1801 ggcgcggcgt gcaggtacat cccgttttcg cccaaacttt cgccgtggtc ggaaacataa
     1861 tgcaccacgc tttccaaatc gtcgcggttt tcaagtttgc ggataacctt gtcgataaac
     1921 tggtccacat acaaaaccgt attgtcgtaa gtgttgacca gcgtggcgcg ggtgcatttg
     1981 ttgatttcgt tggtgtcgca ggtcggcgtg aatttgcgtt cggcttcggt atagcgttcg
     2041 taatacgtcg gcccgtggct gccgatggta tgcaggatta aaaccgcgtc tttatcgttt
     2101 ttgttgagga cttcgtcgaa cttagtcagc aggatattgt cgaggcactc gccgttgcgg
     2161 cagtattcgg gcaggttgag cgaggtaacg tcggtattcg gcactttgcc gcacacgccc
     2221 ttgcagccgg aatcgttttc caaccaggta acttccacgc cggcgcgctg cacgatgtcc
     2281 agcaggttgt cttggtgttc ggctttgatt tcgtcataat ccgtgcggtc gaaggttgag
     2341 aacatacacg gcagggagtg cgcggtcgat gtaccgcagc ttctgacctg cgggaaattg
     2401 acaatttcat cgccgcgcgc ggcaagcagc ggcgtagttt ggcggctgta accgttcaaa
     2461 ccccagttgg cggcacgcgt ggtctcgccc acgaccagca ccacgaaacg gcgcaggctg
     2521 ccggccggcc ggttttgcac gaccgccata tccaattgcg tataaggaat attggaacgc
     2581 ttccaatctt tgtatttcga cacgcccgcg ccgatgaaat tagacggcac aatcagatgg
     2641 gttactgatt tattgttgcg gaaaaacgag gcgtaatcct gatattgcaa cattgcgatg
     2701 cccaacgcgc acaaaaagga aacggcggca agcacaaggc gcgtcaaaag ctccttatac
     2761 caaacgcggt atttaacctt gacggcgata tacgccagcg cgggcaatac gcccaaacat
     2821 acaatccaca gcacatagcc cggcgtaatc aggcgcgcgc tttcggcagc cgtagtttgc
     2881 aagacattat tcaacatcga cttgttgaaa tagatattga aaaatatttc ttggtaagac
     2941 accgccgcac tgataaccaa tatcaacgga atcaatacct tatgcacgaa aggcagggca
     3001 atgacgtgaa aaacgaaatt acttaaaaaa aacagcacca ccggcatcgt atagaggaag
     3061 atatccgccc cggtgccgtt aaaaggatga agctcgacaa ctttggcaaa aaaggcgtaa
     3121 ttcaatacca gcgaggaata cagggaaagg aaggcaatca gcgcggaaga gccgagcttc
     3181 ggcctcaggt tcggttttat catttggaat gtgtcggata agggttggaa aaggcatccg
     3241 gcatttggaa tccggattat tgaaaaagat tcttaattat aaggcaacgg agcaaagcag
     3301 ggcaagaaaa cggaaggcga cgcaggctgt cggatacaca tctcggcaca aacagcatca
     3361 cgcccggatt gaaaaggacg gatagccgcc ggcagcacaa tcttaccacc tccaaaacgc
     3421 cgccggagaa cgccgaaaca gccgatgccg tctgaagccg cttcagacaa catcgggaca
     3481 tcaaccgtaa cgccgttgga aatcgcgcat aaaatctgcc aaagcccgca cgccttcaag
     3541 cggcatcgca ttataaatgc tggcacgcat accgccgacg gttttatagc ccttaagcag
     3601 gcacaagccc tgcaattcgg cttccagcac aaaacggcgg tcaagctcct catcctccgt
     3661 ttggaacacg acattcattt tagaacgcgc attcggacgg atacggttga tatagaaacc
     3721 gccgctgcca tctatcgtct catacaaggt ttgcgccttc agccgattga ccgcttcaat
     3781 ttttttcaca ccgccctgcg cctgcagcca gcggaacacc agccccgaca tataaatcgc
     3841 gtaagttgac ggcgtgttgt acataccgtc gcggttgatg tgcgaacggt agttgaacac
     3901 atcgggaata tcgttcggac aacgctcgag caaatcctcg cggataatga ccacggtcgc
     3961 accggccggg ccgatgtttt tctgtgcgcc tgcgtaaatc agtccgtagt cggcaacatc
     4021 aaactcgcgc gacaaaatct cgctggacat atcgcacacc agcggcggca tgccttctga
     4081 aaggcgcggc acttcacggt attgcagccc gttgaccgtt tcattgacgg caaaatggac
     4141 aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc ggcaggtcga gatagtcgaa
     4201 ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg gtcaaacggc tcatctgttc
     4261 ataagcgata cggctccagt tgcccgttac caccgcgtcg gcagtgcgga aaccgtgtgc
     4321 cagattcatg gctgccatat taaattgggt tgttgctccg ccctgcagaa acaatatctt
     4381 atagttgtca ggcactttca aaagctgcct caaatcctgt tccgcatgat gcaggatgct
     4441 caaaaacatt tccgaacggt ggctcatcgc catcacggga aaacccgtac cgttgtagtc
     4501 caacatttcc tgccgcgccg tttccaacac ggcttcgggc aatacggcag ggccggcgga
     4561 aaaattgtaa atcggataaa gagacatgat gcagccttga ttctgaacaa taacccgcct
     4621 gattttaggc ttgcagcagg ctttgtgcaa ggatgggaaa tacctgtcct ccgccccgct
     4681 tcatgccgcc cgaacacgga aaacaacatc aatatattga tttacaaata taaaaatcat
     4741 gctagcgaca aatagataca tttgttttgt caacaatatt cacgatttcc cattacaaac
     4801 ctcccttaca cccgcttttt tccgtcccaa aaacacaaaa taaatcaaca ctttcatttc
     4861 tccgcaaaag cggttataat cacgccgatt tttcaacttt gacgaaaaat ggccacgggg
     4921 ccattttttt attatgtaca tagggagcag catggatatc caaaccatcc tcgaaaaaac
     4981 cctgcccggc ctgggctacg aactggtcga tttcgaactg accgcgcaag gaacattgcg
     5041 cgtgttcatc gacaaagaag gcggcattac cgtcgaagac tgcgcaaccg tcagcaacca
     5101 cttgagccgc gtatttatgg ttgaagacat cgactacaaa aacctggaaa tttccagccc
     5161 cggactcgac cgccccttga aaaaagccgc cgacttcgtg cgctttgccg gtcagaatgc
     5221 caaaatcaaa acccgcctgc cgatagacgg tcaaaaaaac tttatcggta aaatcgaagg
     5281 ctgcgaaaac gacactgtta ccgtttcctt cgacggcaaa accgtacaaa tcgaattggg
     5341 caacatcgac aaagcccgcc tgcgccccga attcaaattc taaaacacaa caatattgga
     5401 gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa gcactggcaa gcgaaaaaaa
     5461 cgttgatgcc gaagtcgtct tccaagcact ggaattcgcc ctgtctaccg ccgccaagaa
     5521 aaaggcagac cgcgaacaca tggatgtccg cgtacaaatc aaccgcgaca ccggcgaata
     5581 ccaaaccttc cgccgctggc tgattgtcgc cgatgaagac tatacctatc ccgatgtcga
     5641 aaaaaccatc gaggaaatcc aagaggaaat tcccggcact accatccaaa tcggcgaata
     5701 ctacgaagag cagctgccca acgaaggctt cggccgccaa gccgcgcaaa ccgccaaaca
     5761 aatcatcctg caacgcatcc gcgatgccga gcgcgagcag aatctgaacg agtttctcgc
     5821 cgtcaaagaa gacatcgtgt ccggcacggt caaacgcgtc gaacgccacg gcatcatcgt
     5881 cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc gaccaaatga ttccgcgcga
     5941 aaacttccgc agcggcgacc gcatccgcgc cctcttcctg cgcgtcgaag aaatcggcaa
     6001 caccggccgc aaacaagtca tcctgagccg tacttccggc gatttcctcg tcaaactgta
     6061 cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa atccgcgctg tcgcccgcga
     6121 cccgggccag cgtgccaaag tcgccgtcaa agccaacgac cagcgcatcg atccgcaagg
     6181 cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc agcaacgaat tgtccggcga
     6241 gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa ttcgtcatga gcgcgctctc
     6301 acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa cacgccgtcg atgtcatcgt
     6361 tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt caaaacgtgc gccttgcttc
     6421 cgacctgacc ggctggcagc tcaacatcat gacttccgcc gaggcagacg aacgcaatgc
     6481 ggcagaagat gccgccatcc gccgcctgtt tatggatcac ttgaacgtgg acgaagaaac
     6541 cgccgacgta ctggttcagg aaggttttgc aaccttggaa gaagtcgcct atgttcctgc
     6601 cgccgaactg cttgccattg aaggatttga cgaagaaatc gtcgatatgc tccgcaaccg
     6661 cgcccgcgat gccatcctga ccatggcgat tgccgccgaa gaaaaactgg gcgaagtatc
     6721 cgacgatatg cgcaacctcg aaggcgtaga tgccgatatg ctccgcagcc ttgccgaagc
     6781 aggcattacc acccgcgacg acttggcaga gcttgccgtg gacgaactga ttgaaattac
     6841 cggtgtaaac gaagaaaccg caaaagccgt catcctgacc gctcgcgaac actggtttac
     6901 cgaagacaaa taaaaggggg tacagatgag taacacaacc gtagaacaat ttgccgccga
     6961 gctgaaacgc cccgtcgaag acctgttgaa acagttgaaa gaagccggcg tcagcaaaaa
     7021 cagcggcagc gattccctga cgctggacga caaacagctt ctgaacgcct acctgaccaa
     7081 gaaaaacggc agcaacggcg gcaccatcag catccgccgc accaaaaccg aagtcagcac
     7141 cgttgacggc gtaaaagtcg aaacacgcaa acgcggacgc actgtcaaca ttccttctgc
     7201 cgaagaattg gcagcacagg taaaagccgc ccaaacccaa gccgcacctg tccggccgga
     7261 gcagacggca gaagacacgg caaaagcccg agccgaagct gccgcacgtg cagaagcccg
     7321 tgccaaggca gaagcggaag cggcaaaact gaaagcggca aaagcaggca acaaagccaa
     7381 acctgccgcg cagaaaccca ccgaagcaaa agccgaaacc gcacctgttg cggcggaaac
     7441 caaacccgcc gaagaaagca aagcggaaaa agcccaagcc gacaaaatgc cgtctaaaaa
     7501 acccgccgag cccaaagaaa aagccgccaa gccgaaacac gagcgaaacg gcaaaggcaa
     7561 agatgccaaa aaaccagcga aacctgccgc acctgccgtg ccgcaacccg tggtcagcgc
     7621 agaagaacag gcgcaacgcg atgaagaagc acgccgtgcc gccgcacttc gcgcccacca
     7681 ggaagccctg ttgaaagaga aacaggaacg ccaggcacgc cgcgaagcca tgaaacaaca
     7741 ggcagaacaa caggcaaaaa ccgcacagga agccaaaacc ggcagacagc gtcccgccaa
     7801 acctgccgag aaaccgcagg cagccgcgcc ggccgtcgaa aacaagcctg tcaatccggc
     7861 aaaagcgaaa aaagaagacc gccgcaaccg cgatgacgaa ggtcaaggcc gcaacgccaa
     7921 aggcaaaggc ggaaaaggcg ggcgcgaccg caacaatgca cgcaacggcg acgacgagcg
     7981 cgtacgcggc agcaaaaaag gcaaaaaact caaactcgag ccgaatcaac acgccttcca
     8041 agcaccgacc gaacccgtcg ttcatgaagt tttggttccc gaaaccatta ccgttgccga
     8101 tttggcgcac aaaatggcgg tcaaaggcgt ggaagtggtc aaagccctga tgaagatggg
     8161 catgatggtt accatcaacc aatccatcga ccaagacacc gccctgattg tggtggaaga
     8221 actcggccac atcggcaaac ctgccgcagc cgacgaccct gaagcattct tggacgaggg
     8281 cgcggaagcg gtggaagccg aagcattgcc gcgtccgccc gtcgttaccg tcatgggcca
     8341 cgtcgaccac ggcaaaacct cgctgctgga ctacatccgc cgtaccaaag tggtacaggg
     8401 cgaagcaggc ggcattaccc aacacatcgg tgcataccac gttgaaaccc cacgcggcgt
     8461 gattaccttc ttggatactc cgggtcacga agcgtttact gccatgcgtg cgcgcggtgc
     8521 gaaagcaacc gatatcgtga ttcttgtggt cgccgccgac gacggtgtga tgccgcaaac
     8581 catcgaagcg attgcccacg ccaaagcggc aggcgtaccg atggtggttg ccgtcaacaa
     8641 aatcgataaa gaagccgcca acccagagcg tatccgccaa gagctgaccg cacacgaagt
     8701 cgtgcctgac gaatggggcg gcgatgtaca gtttatcgac gtttccgcta aaaaaggcct
     8761 gaacatcgat gcattgctcg aagcagtctt gctcgaagcc gaagttttgg aactgactgc
     8821 ccctgtcgat gcacctgcca aaggtatcat cgttgaggca cgtttggaca aaggccgcgg
     8881 cgcggttgcc acattgctgg ttcaaagcgg tacgctgaaa aaaggcgata tgctgctggc
     8941 cggtacggca ttcggcaaaa tccgcgcgat ggtcgatgaa aacggcaaat ccattaccga
     9001 agccggcccg tccatccccg tcgaaatcct cggcttgtcc gacgtaccga atgcgggtga
     9061 agacgcgatg gtattggcgg acgagaaaaa agcgcgcgaa atcgccctct tccgccaagg
     9121 caaataccgc gacgtgcgcc ttgccaaaca gcaggcggcg aagctggaaa atatgttcaa
     9181 caatatgggc gaaacccagg cccaatcttt gtcggtcatc atcaaggcag acgttcaggg
     9241 ttcttacgag gctttggcgg gcagcctgaa aaaactgtcc accgacgagg tgaaagtgaa
     9301 cgtattacac agcggcgtgg gcggcattac cgaatcagat gtcaaccttg ccatcgcttc
     9361 gggcgcgttc attatcggct ttaacgtgcg tgcagatgcc tcttcgcgca aacttgccga
     9421 aaatgaaaac gtggaaatcc gctactacaa catcatctac gatgccatcg acgacgtgaa
     9481 ggcggcgatg agcggcatgc tttctccgga agagaaagag caagtaaccg gtacggtcga
     9541 aatccgtcag gtcatctccg tttccaaggt cggcaacatt gcaggctgta tggttaccga
     9601 cggcgtggtc aaacgcgatt cccatgtccg cctcatccgc aacaacgtgg tcatccacac
     9661 gggcgaactg gcttcgttga aacgctataa agacgacgtc aaagaagtcc gtatgggctt
     9721 tgagtgcggc ctgatgctca aaggctacaa cgaaatcatg gaaggcgacc aactggaatg
     9781 cttcgacatc gtcgaagtgg cacgcaccct gtgattttga tgcggaaaaa tgccgtctga
     9841 agcgttcaga cggcatacgg aacgggttct gtatcataca gaacccgttt tttatcgcaa
     9901 atcggcttca gacagccctc ctgccttatc ccgatttgaa tctgacttgc catacaaaca
     9961 ggcttcagac ggcattatag tgaattaaat ttaaaccggt acagcgttgg ctcgccttgc
    10021 cgtactattt gtactgtctg cggctcgccg ccttgtcctg atttaaattt aattcactat
    10081 attttcccga caggcggatt tgaagtttct ccacatatcc ggcgcgttcg gcgcggctgg
    10141 tttcggggcg gtgcgtattg agcgacgacc atttccaatg actgcgggct ttgttgagtt
    10201 cgggcgggag tctggcggcg tcccacgggg ctttgcggct gtgcagctcg atatccgact
    10261 gtgccgcgtg tccgcgcgtt tgcaggacgt ggagcaaatc gagggcgcgg gcggcgagca
    10321 gggtcaaggt ttcagggtcg gtgtgcaagg tttggcggcc ggagagtttg tcggaaatgg
    10381 tgcgggtatt gggcaggatg ccgcccaaaa agccgccgat tgccgtaccc aatccgagcg
    10441 agccgccgag tgtggcgatg tccagcccca agccgatgag cgcgccggtt gccgcgcccg
    10501 tgccggtgcg gatgccgtat tgtttgagca attcgctgtc gaacgggtct tggcggaagg
    10561 cttgcggcat ccagtcgccg ccgtcgattt cgctgtggta gaaacggtag agggcaaaca
    10621 gccgctgctg catctgccgt tcgagttggc gtatttccgc ctgcatggtt tgtagcacgg
    10681 tggcggtatc ctcgttttcg tccacttcct gcctgaaggc ggcggcatca attaaaaagt
    10741 cggcgatttc gcggcgcgct tcgccgtcca gccgctgcca ttcgcgccgg cgcatggctg
    10801 tcaggcggtc aagtgtgctg cgttcgggca acatggtggc gaggttttcc cacaggcgta
    10861 gttcgccttc aaaatcaaag gcgacggtgt cgaaccctgc gaaaacgtgc aggtttctcc
    10921 tcgccagcat ggttgtccac gattcgggag gctgtccgcc ggtaaagttg aacacgggca
    10981 taaccggttt ggcacaccat gaaaggatgg tcaactcgtc cctgtatttg tcgaggacgg
    11041 gttcgcgcgc gtcgatgacg tacattgcca tatcgctttg caagacttgc cgtaagactt
    11101 tggcttcctg attgaaatca tggtgcgcgc cgtggctgcc gagaaactgt tgcagccgtt
    11161 cgatgccgtc tgaacgattg tccgtatggt tttccagcca ttccagcacg ccgcctgcgt
    11221 cttcgagtcc gggcgtgtcg tacaggaaaa ccagcgtgtc tgcgccgtcg ctgatggcgg
    11281 cttcttcgac atgacgcgtg gtcgatgggg cgtttctgac ttcgccgaaa ccgctgtcgc
    11341 gcaacagggt acgaaggagc gaggttttgc cggtgttggt atgcccgacg acggcgaggg
    11401 aaaggggttg tttgttcatg atgtttttga agaatggatt ttcagacggt cttttttcag
    11461 aatggcggct taacagaaca tttcaagtga gtttattggt ctttcaaacg cccttcctgc
    11521 gccgccctgt caggctcaag ccacgccgcg ccgcattcgg ccagcgcgtt acgccaatgt
    11581 tccagctttt ccgaaaggtc gtctgaaagc ccctgttccg ccaaaagctg caccaccgcg
    11641 ccgccctgcg ccgcttccga aagtcggacg atctgccgca acacgccgcg gtcgggcaca
    11701 gtttgggcgc gcacgccgat aagcagttgc gccggtttct gcttcagctc tgtctccagc
    11761 gcggcaacct gttcccgatt ggcggcaacg cccttatcca gccattcctg cgccagcctg
    11821 ccctcgaacc attcgccgtc ctgccattcg gtctccagca tgaccgccca tttcggcgca
    11881 tcgttcaaga cgattttcgg cgaaacggcg gacacggttt cccgacgcgt atccgcatcg
    11941 gtgattttgt tctgccagcg gcggatgacc gcctgataat agggtttttc caaatccaat
    12001 ccgttttcgc ttgttttcaa aaggattttg cataccgccc aagccaagag gcgcggcagg
    12061 atgccgtagc aggcgatact gccgaccagc agccccgacc aagcccgcgc atcggcaata
    12121 ttgccgttca gacgaccttc gatgaccgcc cgcgcatcag gcacgggaaa acccagtttc
    12181 gcaggcagcc atgccaacat ttccaccgcg cgtaccgaag cggcattgct caacagcgtg
    12241 ctttcccagt tgaacgtata ttgccgcacc aaaagcagca acaataccga caccagcatt
    12301 ccgagcagcg tgcagagcca caggctgtgc gacgttgcgc ctattttcca acgtaccgaa
    12361 ggttgccgcc actcgtccgc atacagccgc aacaccgcct gatttacagg gtctttgccc
    12421 cgaaaccacg tcgccggact gctgaaaaaa cgccccactt tcacgcgcag gaacaacatt
    12481 gccaaccata ctgccagcat cagcgtattc atgcccaaca cgcccgccaa aaccaaaaag
    12541 aaattcagac cctgattgtc cattagaaga taagtacctg aaaaaccggt aaaaaacgca
    12601 aacgtcgccg ccgccaccca caaccagaac gaccccgcac gcacacgttc caacgtctcc
    12661 cgcagcatac ggttcctgtc aatcatctcc gcccgacgga tgattttttc ctccgtactg
    12721 ccgtccacgc ggcgcaaagc ctccgtcgcc tgcacgggat cgccgctgaa aataaaaccg
    12781 ccttcgtcca aaatacggat cagctcaacc agttttcggg atggattcaa cataaaatgc
    12841 cgtctgaaaa taaaaaacag attttaacac acgcattttc aagaatattc acagtgtagg
    12901 caaagagtaa atctcacaca gaagcaaaag tatcggcgta aactgactgc ctctactttc
    12961 ccgaaagatt gtgcgatgta tacaggcgaa cgcttcaata cttacagcca tttgagcggt
    13021 ttgattctgg cggcggcagg tttggcgctg atgctgctga aaaccatagg acacggggac
    13081 ggctaccgta tcttcagcgt atcggtttac ggcatcagcc ttcttctgct ctatttgagt
    13141 tcctcgctgt accacggaat tgcagccgga aaactgaaaa gcattttgaa aaaaaccgac
    13201 cactgcatga tttatgtcct gattgccgga agctacacac cgtttgcact ggtttctttg
    13261 agaaacgggc cgggctggac ggtattttca ctgtcctggc tgctggcggc tgcaggaatc
    13321 gcacaagaac tcaccatcgg acggaaaagc gaaaaacgac tgctgtctat tgcgatttat
    13381 gtcgtcatgg gttggatggt cttggcggta atgaaatccc tgacagcctc actcccgccg
    13441 gcaggactgg cttggctggc ggcaggcggt atgctgtaca gcgtcggcat ttactggttt
    13501 gtaaacgatg aaaaaatccg acacgggcac ggaatctggc atctgttcgt attgggcggc
    13561 agcatcaccc aatttgtcag cgtgtacggt tacgtaatct gaatgccgtc tgaaaagcaa
    13621 aacctcccga tcctgaagat tgggaggttt tctgtttgcc ggacatcagc ccttgtcgtg
    13681 gaactcgtga aattcatact gataggacaa atcccgaccc gcttttttct gtgccaaata
    13741 atcatcataa atggcacgga tttccttacg caacaaaaac agggctatca agttcgggat
    13801 aaccataaag ccgttgaaca tatcggacag gctccaaacc aaatcgactt tgccgagcgt
    13861 acccaaaacg atggcaagca gaaccaatgc gcgatagatg cccaagtgtc ttcccctgaa
    13921 aagaaaacgg atgttggact caccgaaata ataccagccg ataatggtcg tcaaggcgaa
    13981 aaaggtcaga cacacggcaa gcaattgcga accgaagccc ggaaatgcct tgttaaaggc
    14041 aaattgagta accgccgcgc cctgttcgcc cgaaaggttg gcatcggtca gcaggataat
    14101 cagcgcagtt gccgtacata ccaaaatcgt atcgataaat acaccgacaa atgccgccat
    14161 accttgctgc acaggatgct tcacatccgc agtcgcgtgg gcgtgcgggg tagaacccat
    14221 ccccgcttcg ttggaaaaca gaccgcgcgc cacgccgaaa cggacggctt cgcgcatacc
    14281 gatacccgcc gcaccgccca aaacggcttc gggattgaag gcggcggtaa agatgtggtt
    14341 gaacatcggc acaatatggt cggaaaattc aaacaggata acgacggcgc acaaaatata
    14401 aacaaccgcc ataaacggca cgacaaattg ggcgatattg gcaatacggt tcacgccgcc
    14461 aatcacaacc atgcccgcaa ggacggcaag cacaataccg actgccaaag aaggcacatc
    14521 aaatgcaatg gtaacggcag aagcaatgga gtttgcctgt gtcgcattac cgataaagcc
    14581 caatgcgata atcaacgcaa tggaaaagaa accggacaaa aaacgcgccg cgcccctgcc
    14641 gattttcgga gtcagaccgt gggtgatgta gaacgccggc ccgccgatgt atttgccgtg
    14701 gctgacgacg cggtatttct gcgccagcag tgcctccgca aaaatcgtgg acatccccaa
    14761 aacggcagaa acccacatcc aaaaaatcgc gcccggcccg cctgcggtga tggcggtcgc
    14821 cacgccggcg acgttgcccg taccgatttg cgcagatatg gcaaccgcca acgcctgaaa
    14881 ctgcgataaa gacttgtcgt ctttatcgcc tttggcaaac aagccgccga atacggattt
    14941 gaatcccgcg cccagcttgg taatctgcgg cgcaccaaga tacagcgtaa aaaacaggcc
    15001 gatacccaaa agcgcgtaaa tcagcaggta gtcccaaagg aaccgattga ctgtacccac
    15061 cagaacagac aatatatttt ccataaaata aaccttatct tacaattaaa atgactgcct
    15121 tccaaaagac attccaataa ggaaacacgg cgagcagacc gtatttgccg caacagatgc
    15181 cttaaattgt caacaatcgg ggagaagctg cgccatcata ccgtaaaata tcgttaatta
    15241 aaacatttct ttatttttaa gcggaaagcg gaggaaatcg ctttcagacg gcatcgacaa
    15301 cggcacggca taaaacagga tattttgggt acttgcaact tatgttaaaa tgccgaccgt
    15361 aaaaaatctg acaaaaacag attaattatt tgaaataaga aaggaaattt atggcaggcc
    15421 atagcaagtg ggcgaatatc cagcataaaa aagcccgtca ggatgccaaa cgcggcaaaa
    15481 tcttcacccg tttaatcaaa gaaatcaccg ttgcggcgcg tatgggcggc ggcgaccccg
    15541 gttcaaatcc gcgcctgcgc ctggctttgg aaaaagcagc cgaaaacaat atgcccaaag
    15601 acaatgtgca acgcgccatc gacaaaggta cgggcaactt ggaaggcgtg gaatacatcg
    15661 aattgcgcta cgaaggctac ggcatcggcg gcgcggcttt gatggtggac tgcctgaccg
    15721 acaacaaaac ccgcaccgtt gccgacgtgc gccacgcgtt taccaaaaac ggcggtaact
    15781 tgggtactga cggctgcgtg gcgttcaact tcgtgcatca gggctatttg gtattcgaac
    15841 ccggcgttga cgaagacgcg ctgatggaag cggctttgga agccggtgcg gaagacgtgg
    15901 ttaccaacga cgacggttcc atcgaagtca ttaccgcgcc aaacgattgg gcgggcgtaa
    15961 aatccgcttt ggaggcggca ggttacaaat ctgttgacgg cgacgttacg atgcgcgccc
    16021 aaaacgaaac cgaactctcc ggcgacgatg ccgtcaaaat gcaaaaactg attgacgcgc
    16081 tggaagactt ggacgacgtg caagacgttt acacttccgc cgtattgaat ctggactgat
    16141 acgcagcaca gcagacatac aataaaatgc cgtctgaacc tttcagatgg cattgattta
    16201 tttagccctt gcccacgccc accaaaccgt caggacaacc gccagaaaat acgccgcgct
    16261 ccaaacaggc aaagcaaccc ctaacagata atccggttcg gcacaatttc cgaacccgcg
    16321 cacgacaggc tcgaaccaat caaacaaaga ccagcccttc aagcggaacg tccacggcgc
    16381 accgcacgaa ggagccgtac ccggcggcag cgactgcaac cacaactgat atgccgcaac
    16441 agaaataccc gtaacggccg gaatgctgat aaagacagca ccgaacaaac cgcccgccct
    16501 tcttcttggt ctgcacatca ggacaattgc cgtacacaat gcggttgcca aaacgcataa
    16561 ccgctgactg atacacaaaa cgcaaggctc catacccaaa acatactgtg ccgccaaaga
    16621 accggcaaat gcacagaccg aaacggcaaa cagcagccaa acggcttttc taaataacgg
    16681 ggtcattttc tcaacacacc aatcaaaata ccgatatgcc gattttgctg gatatatccc
    16741 gagatggcaa gggacgaatc gacaccggct caggtgcgga cgatttcggt tttccccgat
    16801 atgcacaaaa cctgacaagg gacaaaacgg cgatttgccc gcacaatgcc cacaaaaaaa
    16861 tacatccgga ggatttgaat ttcagcaaat ttagcgaatc aaaagtttat ttcaatgaaa
    16921 tcatatgatt tttttgaata agcggattga tgggtttttg aaggaatttg ttaccggata
    16981 gccatcgggc aagttttttg caaaatttga atcggtcggg taaattttca aaaaataatt
    17041 gacagcggat aagaaacggc ggataattcc cgccgtcgag ttgcttgatg cagcttgatt
    17101 tttctcctct atttctcctt tgtagacttg gcacacattc aaccggatgt gtgcattttt
    17161 ttatctccgc tttttttgaa atttaattac ttttattgtt tgaaattcca ttctttaaga
    17221 aatttaacaa gaaaaacgct tgctttttga ccggaaaagc tgcataattc gttccgttag
    17281 ctggttcgag tagtcagtta atagtttctc ctctatttct cctttgtaga cttggcacac
    17341 attctattga atgtgtgcat ttttttatct gaagcaacaa gcctctgtgc gtgatgttgt
    17401 tatgtttcat ttaggtgtca aaccgcatag ccagcctgaa atattcaatc caaatccaaa
    17461 accggatttt ctttgacctc ctccatcaca acataactcc tactctccga agcggcaggc
    17521 agttgcaata ggatattgcc tagcatatcc cgataggcag acatatcggg caaacgtact
    17581 ttaatcagat agtcgtattc gcccgacacc aagtggcatt ccataatttg cggaattttc
    17641 agcacttctt ttttgaaatc ttcgaaaata ttgcccgatt tggattgcag cttcagctcg
    17701 acaaaaacca atagtggttt gcccaacaga tggggattga gatgggcgtg atagccggaa
    17761 atataatgtt cccgctccaa acggcgcacc ctctctgtaa cgggcgtggt ggacaagcct
    17821 accttctcgg caagctccgt catcgggatg cgggcattct gttggaggat cttaagaatg
    17881 cggaaatcga ttttatctag ttctttcatt tggattacct tgtatttatt attgatttta
    17941 acaaatagag tatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta
    18001 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatat
    18061 ttgggaaagc gattatatca ggaaaagcaa accgccttcc tacctgaaaa ctgctgcttc
    18121 ggcttgaaga cacaagggtc tttaatattt taaaagcctt gccgttggat tataattccc
    18181 taattgattt cttaattttg ctaataaaca cttgcttggt aaggaatgaa tttatgcgcc
    18241 ctttgaacgt gcagatcagg ttgggcaacc ttaggcacaa ttatcggatt ttaaaggaaa
    18301 tgcacggagg caagctgttg gcggtagtga aggccgacgc atacggacac ggtgcggtca
    18361 gatgtgcttt cgcgctggca gacttggcag acggctttgc cgtggcgaca atcgatgaag
    18421 gaatcaggct gcgggagagc ggcattaccc atccgattgt ccttttggaa ggcgtatttg
    18481 aagcatcaga gtacgaagcg gtcgaacaat actcgctctg gccggcagtc ggaaaccaat
    18541 ggcaacttga ggctttgctt tcccgccatt ggaaaaaacc tgtcaaggtc tggttgaaaa
    18601 tggattcggg aatgcaccgt accggatttt tccctcatga ttacgcttcg gcatatgcgg
    18661 cattgaaaca gtcggaatat gtggacagta ttgtcaaatt ctcgcatttc tcctgtgcgg
    18721 acgaacccga aagcggtatg acggaaatac agatggaagc attcgatttg ggtacggaag
    18781 ggctggaagg cgaagaaagc cttgccaatt cggcggctat tttgaatgtt cccgaagcac
    18841 gcagggattg ggggcgcgcg ggcttggcgt tatacggcat ttccccgttc ggaggcagcg
    18901 atgacaggct gaaacccgtg atgaggcttt caacccgtat ttttggcgaa cgcgttttac
    18961 agccgcactc ccctatcggt tatggcgcaa cattttatac cagcaaatct acgcgcgtcg
    19021 gtctgattgc ctgcggttat gcggacggtt atccgcgccg cgctccaagc aattcccccg
    19081 tcgccgtcga cggtaaattg acccgggtca tcggcagggt ctctatggat atgatgacca
    19141 tcgagctgga cgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg ggcgatacgg
    19201 tcaacatcaa tactgttgcc gaagcggccg gaaccatccc ttacgaattg atgtgcaata
    19261 tcaaacgtgc aaaattcact tatatcgagt aatcgagtcc aaacaaaaat gccgtctgaa
    19321 gcctttcaga cggcatttcc ccatcaaaac cgcaatcagt ttttcattga ttgaaccgga
    19381 gccgggattc tgccgccccg tttgacgaac acttcgcacg aaccttcttt gaccggcatt
    19441 acaggcgcgt agcctaacag gccgccgaac tcgacactgt cgccgacggt tttaccggtt
    19501 accggaataa tgcgcacggc agtggttttg ctgttgatca tgccgatggc ggcttcgtcg
    19561 gcaatgatgc cggaaatggt gtgcgcgggt gtgtcgccgg gaacggcaat catatccaag
    19621 ccgaccgaac aaacggcggt catcgcttcg agtttatcca acgtcagcac gcctgcttcg
    19681 gcggcggcaa tcataccttc gtcttcggaa acggggataa acgcgccact caaaccgcca
    19741 accgcgctcg aagccatcat gccgcccttt ttcacggcat cgttcagcaa tgccaaagct
    19801 gctgttgtgc cgtgcgtacc gcagacgctc aaacccattt cttcaagaat gcgcgccacc
    19861 gagtcgccga cggcaggggt cggtgccagc gacaagtcga gaataccaaa cgggatattc
    19921 agcatttttg aggcttcgcg gccgatgagt tcgcccacgc gggtaatttt gaaggcggtt
    19981 ttcttcacaa cttcggcaac ttcggtcaat gtcgttgcat ccgaattttc caacgcggct
    20041 tttacgacac ctgggccgga tacgccgaca ttaatcacag catccgcttc gcccgatccg
    20101 tgaaacgcac ccgccataaa cggattgtct tccaccgcat tgcagaacac gacgattttc
    20161 gcgcagccga aaccttcggg cgtaatttca gccgtgcgtt tgatggtttc gcctgccagc
    20221 ttgaccgcat ccatattgat accggcgcgc gtactgccga cattgatgga gctgcacacg
    20281 atgtcggtgg tcttcatcgc ttcgggaatg gaacggatta acacctcgtc tgaaggcgac
    20341 atgccttttt gcaccaacgc ggaaaagccg ccgataaagg acacgccgat ggctttggca
    20401 gccttatcca aagtttgcgc cacgctgaca taggaatcgg catgggtagc cgccgcgatt
    20461 tgggcaatcg gcgtaacgga aatgcgctga ttcacaatcg gcacgccgta tttggcagac
    20521 agatattttg ccgtcgccac caagtctttg ccgaccgtgg taattttgtt gtaaatgttt
    20581 tggttcaaca cattgatatc gctgctgatg cagtcgtgca gatcaatgcc gatggtaatg
    20641 gtgcggacat caaaattctg gtcggcaacc attttgacgg tttctaaaat ttcgccggat
    20701 tggatactca tcacattcct ccgactcaaa tgcggtgcat cgcttggaag atttcttcat
    20761 tttgcatacg gatatcaagc gcgagttttt tgctctcttc cgcaaacaaa tccaaaacct
    20821 cctgccgaga tttggggcat ttcgaggtat ccaccaagat aatcatggta aaaaaatcat
    20881 ccatcagctg ttggctgata ttgaggatgt tgattcggtt ttccgccaaa attttggaaa
    20941 catcgtacac gatgccgacg cggtctttac cgatgacggt gatgactgaa ttgttcacag
    21001 gcttactcct tgcagatatc cgttaaagtc cgaaattata ccaccgttgg attttgaaga
    21061 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
    21121 gattcagggt tcgattaaat aaccatcctt atcccactgg gttttcctga ccaacttgtc
    21181 atcctgataa acagcttcac tctttttaga accatcttca taccactcta agactactcc
    21241 attacgttta tgatgatgga tagacagttc cgagagtaat cgaccgcttt catcccatgt
    21301 cagaatcttg gccggctcat cattgaccat aaccatttcc gttttgatat tgccatcagc
    21361 ataccattgt ttccatacgc cgtttgcctt attttgttta aactggattt cgctttcctt
    21421 gctgccatta cggtaatagc ggtaccccgt accttcactc aaaccatttt tataaggcat
    21481 aaccgcagat tttttaccgt tcggatacca gttgacccac tcaccgtccg gcttaccctt
    21541 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
    21601 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
    21661 tttcttcatc gacggataat aaaaatcctg cgcgtacgca ataccctccg ccacactata
    21721 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
    21781 aacagaatag gtctgtgccg ataaagaagc cgaaaaaccc aacaggacaa ttgaaaatac
    21841 aatccgagat aattttttca ttgcaatagc gatataaaaa caaggctgtg ttttagtaat
    21901 ctgttgattt caattatttg caagggaaaa gacaattatt ttccggttag gaataaacct
    21961 attctactga atatattgaa gccaagtacg cctatcaaca ctatattaaa acactgccaa
    22021 aaacaattaa cttataaaca atatggtaag gatttctctg ccaagcatca aacccgagac
    22081 aacgtatcgt aaaaatgccg tctgaaaaca aatcgtcttc agacggcatt tccccttcaa
    22141 ctcactcttc acccaataac tgctcgcgcg tcaagaggaa aacaaaaccg tcgcccccgc
    22201 tggtttccaa ccaagtaaaa ggcaactccg gatacgctgc ttccaataca tccctgttat
    22261 gcccgatttc caccagcaat acacctttgg gattcagaaa ctttgccgca ttcagaagaa
    22321 tctgcctggt ggcatccaac ccgtccgccc cgctgcccaa tgccaattcc ggttcgtgca
    22381 aatactcttc aggcaataac tcaaccgatt ccgcatccac ataaggcgga ttggaaacaa
    22441 tcaaatcata agtgccttcc aatccttcaa acaaatccgt atgaataagc cggatgcgtt
    22501 cttccaaacc ataatcttcg acattaatcc ctgccacttc caaagcatcc aagctcacat
    22561 caaccgcatc aatttgggca tcaggataat gatgcgccat ctgaatggca aggcaaccgc
    22621 ttccggtgca aagatccaaa gcattatgca ccaactcatc gtattctatc caaggacgaa
    22681 gtccgtcacc caacaattca taaataaaag aacgaggtat gattacgcgc tcatccacat
    22741 agaaatcaaa ctctccctgc catgcctggt gtgtcaaata agcggctgga atgtgttcga
    22801 cagcacgacg ctcaataacc gtcagcactt cctctttttc agcttccaag agttttgcat
    22861 caagatatgg ggcaagcata tccaaaggca aattcaaagt atgcagaatc aaataagctg
    22921 cttcatcatg cgcattatcc gttccatgac caaaaaagag ccctgcctca ttaaaacggc
    22981 tgactgcaaa acgtaaaaca tcgcggatag tcgtcaattc ttgtgctgcc tgattaaaca
    23041 taatatgaac cattctgcgt atagatactt ttaattataa cagaaacaac aagcaaacct
    23101 tttcatatcg ccaaataacc acccaatcta cccatacaac tacataaatg cccgcgcgaa
    23161 aaccatcgcc cgaacggaaa cgacaatggc cgacggtatg ggcaacctga ttggctggga
    23221 aaaaacgggg cttgttgccg gtaagcagtg gataaccgca aaagacgaca aggtgtccga
    23281 tgtctgcaat gccaacggcg agatgggcgt aatcgggctt tacgagcctt tctcacacgg
    23341 cgcattgacg atacccggtc atccgaactg ccgatgcgag gttgtttccg tatcgggtgg
    23401 cgaattgggg gaatttgccg aaaaaaagga gcttcgtaaa gcggctatgc agtatgcgcg
    23461 ggataacttt atcggcaaaa gctatgtcaa tgaaaacagc gggcatgaac tgaaggtaac
    23521 ttggcaaggt gtgaaacacg ctgcgtcaaa ggcaaatcag gcggaattat ccatcatgac
    23581 aaaacttgat gacttattgc gctacgcaaa atatgagggt tcttattcgg ataggaaagg
    23641 tcatcctaat attattgcag cacataagta tcgtgccgtt gccaaggttg ggaatgagtc
    23701 tttaaatatc ggtgtgattg taagggaatt tccagacgac cataaacatt acgaccattt
    23761 catcttgaag gatgaataaa gcccttttgc agtgtcgttc tggagcggat agcgttaagg
    23821 caagtacact tccagccttg aaaaagggct ttaaattcag catgccattt atacaggcag
    23881 gagtaaaccc atgacaaagt tatacgcaga aatcgccaag atggagacgc aggacgacga
    23941 cacggtcaag gtttggggtt acgcttcaag cgaggaaatc gattcggacg gcgaagtcat
    24001 cgcgacggca gctatgaagg cggcgattcc cgattatatg aagtttggcg aggggcgcga
    24061 gatgcacggc tcaaacgctg cgggaacggc aattgaaatc aacgttgaag acgacggcat
    24121 aacctttttc ggtgctcata tcatcgaccc cgttgtcgtg gcgaaagtca aaacaggcgt
    24181 ttacaaaggc ttttccatcg gcggcagcgt taccgcccgc gatgatttga acaagtccca
    24241 aatcacgggc ttgaagctga cggaaatcag ccttgtcgac cgccctgcca atcccgatgc
    24301 ggtgtttacc tgctttaagg cggacaaagg tgcggaagcg gtaaacaacg atacagaaca
    24361 taatgctaca tattt
//
LOCUS       DAXIHS010000023        23960 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXIHS010000023 DAXIHS010000000
VERSION     DAXIHS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.023"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(61..1947)
                     /gene="uvrC"
                     /locus_tag="ACFOF2_000818"
     CDS             complement(61..1947)
                     /gene="uvrC"
                     /locus_tag="ACFOF2_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC7107169.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYHDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2002..4164)
                     /locus_tag="ACFOF2_000819"
     CDS             complement(2002..4164)
                     /locus_tag="ACFOF2_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC7107170.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMYAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4303..5253
                     /gene="trxB"
                     /locus_tag="ACFOF2_000820"
     CDS             4303..5253
                     /gene="trxB"
                     /locus_tag="ACFOF2_000820"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC7107171.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5409..5777
                     /gene="rpsF"
                     /locus_tag="ACFOF2_000821"
     CDS             5409..5777
                     /gene="rpsF"
                     /locus_tag="ACFOF2_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC7107172.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5778..6080
                     /gene="priB"
                     /locus_tag="ACFOF2_000822"
     CDS             5778..6080
                     /gene="priB"
                     /locus_tag="ACFOF2_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC7107173.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6087..6317
                     /gene="rpsR"
                     /locus_tag="ACFOF2_000823"
     CDS             6087..6317
                     /gene="rpsR"
                     /locus_tag="ACFOF2_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC7107174.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6334..6786
                     /gene="rplI"
                     /locus_tag="ACFOF2_000824"
     CDS             6334..6786
                     /gene="rplI"
                     /locus_tag="ACFOF2_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC7107175.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6800..6907
                     /locus_tag="ACFOF2_000825"
     CDS             6800..6907
                     /locus_tag="ACFOF2_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC7107176.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            7482..7637
                     /locus_tag="ACFOF2_000826"
     CDS             7482..7637
                     /locus_tag="ACFOF2_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107177.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(7753..8559)
                     /locus_tag="ACFOF2_000827"
     CDS             complement(7753..8559)
                     /locus_tag="ACFOF2_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC7107178.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8560..9306)
                     /gene="pssA"
                     /locus_tag="ACFOF2_000828"
     CDS             complement(8560..9306)
                     /gene="pssA"
                     /locus_tag="ACFOF2_000828"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC7107179.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(9380..9505)
                     /locus_tag="ACFOF2_000829"
     CDS             complement(9380..9505)
                     /locus_tag="ACFOF2_000829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107180.1"
                     /translation="MPSNNIHPAKIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9889..10371
                     /locus_tag="ACFOF2_000830"
     CDS             9889..10371
                     /locus_tag="ACFOF2_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC7107181.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10423..11634)
                     /locus_tag="ACFOF2_000831"
     CDS             complement(10423..11634)
                     /locus_tag="ACFOF2_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC7107182.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11857..14295
                     /locus_tag="ACFOF2_000832"
     CDS             11857..14295
                     /locus_tag="ACFOF2_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC7107183.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            14504..15814
                     /gene="tig"
                     /locus_tag="ACFOF2_000833"
     CDS             14504..15814
                     /gene="tig"
                     /locus_tag="ACFOF2_000833"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC7107184.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15910..16524
                     /gene="clpP"
                     /locus_tag="ACFOF2_000834"
     CDS             15910..16524
                     /gene="clpP"
                     /locus_tag="ACFOF2_000834"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC7107185.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(16581..17846)
                     /gene="ispG"
                     /locus_tag="ACFOF2_000835"
                     /gene_synonym="gcpE"
     CDS             complement(16581..17846)
                     /gene="ispG"
                     /locus_tag="ACFOF2_000835"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC7107186.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSVWRTAYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17862..18623)
                     /gene="pilW"
                     /locus_tag="ACFOF2_000836"
     CDS             complement(17862..18623)
                     /gene="pilW"
                     /locus_tag="ACFOF2_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC7107187.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQKSFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18626..19720)
                     /gene="rlmN"
                     /locus_tag="ACFOF2_000837"
     CDS             complement(18626..19720)
                     /gene="rlmN"
                     /locus_tag="ACFOF2_000837"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC7107188.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19864..20289)
                     /gene="ndk"
                     /locus_tag="ACFOF2_000838"
     CDS             complement(19864..20289)
                     /gene="ndk"
                     /locus_tag="ACFOF2_000838"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC7107189.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(20348..21499)
                     /gene="zapE"
                     /locus_tag="ACFOF2_000839"
     CDS             complement(20348..21499)
                     /gene="zapE"
                     /locus_tag="ACFOF2_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC7107190.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
     gene            complement(21890..22717)
                     /gene="fghA"
                     /locus_tag="ACFOF2_000840"
     CDS             complement(21890..22717)
                     /gene="fghA"
                     /locus_tag="ACFOF2_000840"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC7107191.1"
                     /translation="MKLIEQHQIFGGLQQVWAHDAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQLYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(22726..23835)
                     /locus_tag="ACFOF2_000841"
     CDS             complement(22726..23835)
                     /locus_tag="ACFOF2_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC7107192.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
BASE COUNT         5798 a         5932 c         6351 g         5879 t
ORIGIN      
        1 tcggtatacc gacctgctgt atcatatttt ttacagacgg attttgtctt tgacagcatg
       61 ctaatgcaga tgattgtaaa tcgtttccgc caatgccttg ctgatgcctt ccactttttc
      121 caagtcctcg cggctggcgg caatcacgcc gcgcagaccg ccgaagcggg tgagcagggc
      181 ttggcggcgt ttgctgccta cgccggggat gtcgcttaag gaggaggtaa cgcgggcttt
      241 gtcgcgtttt ttgcggtgac cggtaatggc gaaacggtgc gattcatcgc gtacggtttg
      301 caatagatgc aaggccgggc tgttgggcgg caggcggaag acttcgccgg taaaaggcag
      361 tatgagctcc tccataccgg ctttgcgctc cgggcctttg gcaatgccga ccaaagggat
      421 gtgcagcccg agttcttccc ataccgatac ggctacgccg atttgccctt tgccgccgtc
      481 aatcaacacg gcatccggcc atttgacggt ctcgccgttg gcttcggcct cctgcatttt
      541 gccgtaacgg cgcgtcaaca cttcgcgcat ggcggcgtag tcgtcgccgg gtttggcggt
      601 cgtgatgttg tagcggcggt attgcgaagg ctggatgttt tgctcatcgt acacaacgca
      661 ggacgcaata gtggcttcgc cttgtgtgtg gctgatgtcg aaacattcaa ggcggttgag
      721 gccgtctgaa tccatgccga ggatttttgc cagttcatca atgcggtgtt gctggctgct
      781 ttgttgcagg cggcgttgtg caatcgccat ttgtgcgttt tgttccgcca ttttcaacca
      841 gactttgcgt tcgcctatgg ttttggtgac aaattgcatc tgcttgccgt gttcgccttc
      901 caaagcctct ttcagcgcat cgggaacgga aaagttgcta ataatgatgt cgggtttgct
      961 tttgcccaaa taatgttggg cgacgaaggc ttcggcgtaa tcttgtccgt ttggctcggg
     1021 atcgtttttg gtgtcgggga aaaagctttt atcgcctaca tgccgtccgc cgcgtatgct
     1081 gacccagtgt acgcagacga ggccgtctga aacggcgagc gcgagcaggt cgatgtcgtt
     1141 ggggttgttc gggtttttgc tgtcgatgaa ctggttgctc tgcataatgc cgagtgcttg
     1201 gatttggtcg cggtagcggg cggcttcttc aaattgcaga ttagcggcgg cggtttgcat
     1261 tttgtgttgc agggtacgcg tcagttcgtc ggttttgcca ttgaggaaag ttgctgcttc
     1321 acgcacgctg tcgtgataat cttcttcgct gatatgaccg acacaaggtg cggtacagcg
     1381 tttgatttgg taaagcagac aaggacggtc gcggtgctcg aatacactgt cttcgcaggt
     1441 acgcagcata aagacttttt gcaacacttg aatactgtcg cgcacggcgt tgctgttggg
     1501 atacgggccg aaatattgat ttggcttttt cagtgtgccg cggtaatacg ccatttgcgg
     1561 atattgatgg ccactgagca tcaaataagg atagcttttg tcatcgcgga aaagaatatt
     1621 gtatttcggc gacagagctt taatgaagtt gttttcgaga atcagtgctt cggcttcgga
     1681 acgcgtgatg gtggtttcga tatgatgcac ctgttttacc atcaatgcga tgcgcggcga
     1741 atggtcgttt ttttggaaat agccggacac gcgccgcttg aggttgaccg ctttgccgac
     1801 gtataagact ttgccgcctt cgtcaaaaaa acggtacacg cccggcagct tgggcaggtt
     1861 tttgaggaag agcgggatgt cgaattgttc cggagaacgg ttttctgtat tcatatcaat
     1921 catttgaaaa acggcttcag acggcatatc ggcagcggca gccttatccg ttcggaaaag
     1981 ctgtcgggtg gttatatgcg gtcaatcgat ttttacccgt ttcaggcgca aggcgttgct
     2041 caacacggaa accgagcttg ccgccattgc cgcgcctgcg atgacagggt ttaaaaaacc
     2101 gagcgcggca agcggaatgc ccaaaatatt gtagaagaag gcgaaaaaca ggttttgctt
     2161 gatgtttctc aacgtcgccc gcgataccga tagggcatcg gcgagttggt tgaccgaatg
     2221 ttgcatcagc gtggcggatg cggtgtgttc ggcaacgtcc gcgccgcctt tcatggcgaa
     2281 gctaacgttg gcggcggcga gcgcaggtgc gtcgttgatg ccgtctccga ccatcgccac
     2341 ggttttgccg gcggctttga gtttctgcac ttcggcggct ttgtcgcgcg gactcatatt
     2401 accgaaggcg tgtgcgatgc ccagttgttt ggcgacgtat tcgaccgtgc cttggttgtc
     2461 gccgctcata atatagacat cgatattgtg ttttttcaga cggcctatgg cttcggcggt
     2521 atcggctttc aacgcgtcgg caagtgcgaa tgcgccgatg ggtttgttat cgactgagac
     2581 cgcaacaatg cttgcaatat cccaaacgcc gtctaaaaac ttcggcaagg ccagttcggc
     2641 aaattcggct ttgcctgctt tcaccaaacc cacgccttcc acttcggcgg taatgcctgc
     2701 gccgacaacg gtttgtgcgt tttgtgcggc gggaatgtcc aaaccgcgcg cttgggcggc
     2761 ggagacgatg gcacgggcga gcggatgggc ggcgttttgt tcgacggcgg cggcgatgcg
     2821 gtacaaagcg tcttcgtcaa agccgctgtc gggaacgcaa taaacggcgg caacctgcgg
     2881 gctgccttcg gtcagcgtac cggttttgtc caacacgacg gcatcgacgt gggcggcttc
     2941 ctccattgct gccgcgtctt taaaccaaat accgtgttta accgctttgc ccataccgac
     3001 cataatcgcg gcaggggttg ccagacccag cgcgcacggg caggcaatca ccaaaacggc
     3061 gacggcgtac atcagcgcaa ccgtccaatc gcccttaatc agccaagtaa caataaaagt
     3121 caacaacgca atgcccacga cggcaggcac gaataccgca gccgctttat cggctacgcg
     3181 cgcaatcggt gctttactgc cttgtgcttc agagagcgcg ttcatcatgt cgccgagctg
     3241 ggtttggctg ccgagctgcg tggcgcggta caccacactg ccttcggtca ttaacgcgcc
     3301 cgccaacact ttgccgcccg cctttttttc ttcaggattg gattcgccgg taagatggct
     3361 ctcgtccgcc caaccgctgc cgctttcaat gatgccgtct gcggcaatgc gttcgccgtg
     3421 gttggcgcgg ataaggtcgc cgatttgcac ttggtcgatg ggaagctgtt tccattcgcc
     3481 gttgcgttgc acgttgactt gggttggtgt aagtttgagc agcaagccca agctgttgag
     3541 gctggatttt ttggtacggt gttccaaaaa tttacccagt gacacaaaac cgatcaccat
     3601 cacgcccact tcaaaataca catgcgccat accgtacgcc gcgtgcgggc tgaaaaacag
     3661 catatagacg gaatacaggt aaatcgagac cgtgccgatg gtaaccagca cgtccatatt
     3721 cgccagtccg cccttaatgc tcgcccacgc gcttttgtaa aacgggattg ccagccaaag
     3781 ctgcaccacg cttgccaatg cgaactgcca caacggcgga atcatccaat cgtgtctgcc
     3841 gatcatcatc cccgccatgc cgataaggaa cgggacgttg atggtgaaca gcagccacag
     3901 ccgccagccg atatggtgtt ctgcttcggg ttgcggcaat gtatcttccg ttttttcctt
     3961 cgcgccgtaa ccggttttct caatgatttt ggcaatgtcg gctactgagg ttttgctgtc
     4021 gtcaaacact acctgcgcct cttcgctggc gaagtttacc cccgccgatt cgacaaaatc
     4081 ttttttgttc aacacttttt caatgcgcga agcgcaggcc tggcaggtca tgccttcgat
     4141 ttggaaacgg attttttgtt gcataagcgt gattcctgct ttgatattca gacggcagtt
     4201 aaggctgcgg gatgccgtct gagcgccggt atggtcgaaa tttctcaaac gcgccgataa
     4261 ttcgagtaga atccgaaaac ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa
     4321 ctgattattt tgggttccgg ccccgccgga tacaccgccg ccgtctatgc cgcgcgtgcc
     4381 aatttaaacc ccgtcattat tacaggtatc gcgcaaggcg ggcaactgat gaccaccacc
     4441 gaagtggaca actggcctgc cgatgccgac ggcgtgcaag ggccggaatt gatggcgcgg
     4501 tttctcgccc acgccgaacg tttcggaacg gaaatcattt ttgaccaaat caacgccgtc
     4561 gatctgcaaa aacgcccgtt tacactcaaa ggcgatatgg gcgagtacac ttgcgatgcc
     4621 ctgattgtcg caacaggcgc gtccgccaaa tacctcggtt tgccgagtga ggaagcgttt
     4681 gcaggaaaag gcgtttccgc ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat
     4741 gttgccgtag ttggcggcgg caatacggca gttgaagagg cactctacct tgccaatatc
     4801 gccaaaaccg ttaccctgat ccaccgccgc agcgagttcc gtgccgaaaa aatcatgatc
     4861 gacaaactga tgaaacgcgt ggaagagggc aaaatcatcc tcaagctgga aagcaacctg
     4921 caagaagtac tgggcgacga tcggggcgta aacggcgcat tattaaaaaa caacgacggt
     4981 tctgagcaac aaattgccgt cagcggcatt tttatcgcca tcgggcacaa gccgaatacc
     5041 gatattttca aaggtcagtt ggaaatggac gaagccggtt acctgaaaac caaaggcggc
     5101 acggcggaca atgtcggcgc aaccaatatc gaaggcgtat gggcggcggg cgacgtaaaa
     5161 gaccatacct accgtcaggc gattaccagc gcggcttccg gctgtcaggc ggctttggac
     5221 gcggaacgct ggctgggcag ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc
     5281 ggaatgcaaa tttttcgcac caaatccttg attccgtcgg caaaccccaa tataatgcct
     5341 gctctttgaa ccttgctttc tgctttcgca tggcagaagg ctgtttttac catccataag
     5401 gagataacat gcgtcattac gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag
     5461 tgcctgctat ggttgaacgt tacaaaacca tgattgccga agcaaacggt aagattcacc
     5521 gtttggaaga ttggggtcgc cgccaactgg cttacccgat taacaaaatc cataaagccc
     5581 attacgtttt gatgaacatc gaaaccactc ccgaagtggt tgaagagctg gaaaccgcat
     5641 tccgcttcaa tgatgccgta ttgcgccatc taaccatcaa aaccaaacac gccgttaccg
     5701 aagcatcccc tatgttgggt ggtgaaaagg ctaagaacct gttgagcggt gcgtctgaag
     5761 aagcggtcgc ccaataattg ggattcaata atcttgtttc gcttgccgcg ttaattgaaa
     5821 aggttttccc tattcgatat acgcctgccg gaatccctgt tttagatatt attttaaagc
     5881 acaaatcgtg gcaggaggaa aacgggcagc aatgccttgt ccaattggaa attccggcac
     5941 ggattttagg caggcaggcg gaagagtggc agtatcggca aggtgtatat gttcacgtcg
     6001 aaggtttttt agctcaaaaa agcagacgtt cccttatgcc gatgctcagg atacaaaata
     6061 ttcaagaata taaaggttaa acgacaatgg ctcgtcaatc attcaaacgt agaaaattct
     6121 gccgtttcac ggctgaaaaa atccaagaag tcgattacaa acaagttgat ttgctgaaag
     6181 actttatctc tgaaaacggt aaaatcattc ctgcacgcat cacaggaacg aaggcattct
     6241 accaacgcca attggctgtt gccgtaaaac gcgcacgctt cctggctctg ctgccttaca
     6301 ccgaccaaca caaataattt tggagattga atcatgcaaa ttattctgtt agaaaaaatc
     6361 ggcggtctgg gcaacttggg cgacatcgta accgttaaaa acggctacgc ccgcaacttt
     6421 ctaattcccg caggtaaggc aaaacgtgcg accgaagcga atatgaaaga gtttgaagca
     6481 cgccgtgccg aattggaagc caaacaggct gaaattttgg cagatgcccg agtccgtcag
     6541 gaaaaattgg acggtcaaac cgttaccgtt gctcaaaaag ccggtgtgga cggccgcctg
     6601 ttcggttccg ttaccaatgc cgacattgct gctgcaatcg ttgctgccgg catcgaagcc
     6661 gtgaaagcaa atgtacgtct gccgaacggt cctctgaaag ccgttggcga gtacgaagtg
     6721 gaagtggctt tgcacactga tgccgttgct aaaattaccg ttgccgtcgt tgccgcaacc
     6781 gagtaattga aattatcaga tgccgtctga aaccctgccg attgtcggaa tgtggtttca
     6841 gacggtattt gttttgtttt ccgtatattc tgcaaaaatc gggatggttg tttttatagt
     6901 ggattaaatt taaaccagta cagcgttgcc tcgccttgcc ttgccgtgct atctgtattg
     6961 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatttgc ctgtggtctt
     7021 tgcaggaata gtttgttaac gaaatttgat gcataaaatg cgccaaaaaa tattggctgt
     7081 gttttaatat agtgttgata agcgtacctg gcttcaatat actcaatagg atagatctct
     7141 tcctaaccga aaaaataatt gtcttttccc ttgcaaataa ttgaaatcaa cagattacta
     7201 aaacacagcc aaaatattca attgacaaaa acttttctga ttttgagaaa tctgtgaaaa
     7261 tcaggggatt atttattttg aaaatggtat cgggcataaa atttggctgt gtttcaatat
     7321 ggtgctgata acatactttg tttcaaaatg ttcaatagga taacttcctg attgaccgaa
     7381 aaataatggc cttttccctt gcaagcagtt gaaatcaaca gattgtcaaa atacatccta
     7441 aaaatttaat aacttatata tatgattgca aaggttgcgg cttgtatacc ggtcattatg
     7501 gcggaaatac cgtttgggag gcagccgcat cgcgtggaat cggcacggca ggcgatgcgc
     7561 cgatgccttc gggcgtgaag gcggggcaat cggcagtttg gaaaaaggca atggaatctg
     7621 tgggcagaag aagttaaggg taaaagtcgg gcgcacggtc aggttgtctg tccttttgtc
     7681 ggaacaggag actgggcagc tttgtcctga ttaattgctt gtgcaaacgg taaaaaggtt
     7741 ttttcgtgtg ttttaaagca ggttgtacag catttttccg gcaatcaaaa gcaacataat
     7801 gccgaaggat tttttgagtt tggcagaaga aagtttgtgg gcggttttga caccgagcgg
     7861 ggcaaaggca atggttgccg cgctgaggac ggcgacggcg ggcaggtaaa ggaagcccag
     7921 tgacccttcg ggcaatcctg caatattcag gccgttgagc agatacgata ttgcgccgga
     7981 gagtgcaatc ggccaggcaa ggccggatga tgtgccgatg gctttatggg cggggaagcc
     8041 gcagtggatt aagaagggga cggaaagtga accgccgcct atgccgaccc agctcgacat
     8101 tgtgccgaac agtgtggaaa ccgcagtcag tccgggcagt ccgggcagcg ggcgggatgc
     8161 cgtctgaggg tcggtatgca gtgttttgaa tgcgacggcg gttaaaaaca ggatgaagaa
     8221 aatttgaagc ccgaacgcgg ggatatattt tgcggagagt gcgcccgtga atacgccgaa
     8281 tatcataccc ggcatcatcg caaatacggt tttccagtcg accgcctgtt ttttgtgctg
     8341 ccccaacata ctggaaaagg cggtgaagac catgacggcg aaggatgtgc cgacggcgag
     8401 gtgttgcgcg taaggatgtt gtgccaaacc ctgcaaatca agtacccata aaacgacagg
     8461 gacaatcagc gtgccgccgc ctacgccgaa cagaccggca ataaaacctg ccgcactgcc
     8521 tacggcaagc aggattaaga taatgtccca atgccacatt taatccgcct ttctgtattt
     8581 tttccagaat cggcgtgccg ccataatgta gccggacagg ctgtatccga gaaagaacag
     8641 gaagaggacg agcgacggtt cccaagtgac cagaagcagc aataagacgg caagcagcat
     8701 tccgacaaag gggacttgtc tgcggatgtt gatttcttta aaactccaaa aagggatttg
     8761 gacaatcatc gacaggccgg caaacagtgt gatgcccaat gcccaccagt ggacggcggg
     8821 gaatttttcg acgctgtggt tgacccaaat cagcccgaca atcagcgcgg cggcagtcgg
     8881 actgggcacg ccgataaacc agcgtttgtc caccttgccg atgagtgtgt tgaacagggc
     8941 gaggcgcagg gcggcgcagg cgcagtagat gaaggcgacg gaataaccga ttttgccgaa
     9001 ctgccaaagc tgccatttgt aggcaatcag agcgggagca acgccgaagc tgaccatatc
     9061 ggcaaggctg tcgagctgct ccccgaacgc gctttggctg ttggtcagcc gcgccacgcg
     9121 cccgtccata ccgtccagca acatagagat gaataccgcg atggcggcgg tttcataacg
     9181 tccgtgcatg gattgggtga ttgcgtaaaa tgcggaaaac agcgcggcga tggtaaagga
     9241 gttgggcagc aggtagatac tgttttgacg cagggagcgt tgcggggtgt tggtcggggt
     9301 ttccatagct gaagtgtctc acggacgtgg agaatcgata ggggattata acccaaacca
     9361 ttatcgaaca atacgccctt taggatgaag ccctcggagg cggtttgttt ttctgatggc
     9421 ggatgctttg cggggcggtt tgactctgcc tgccaccggg tgtgttgttt cggatttttg
     9481 ccggatggat attgttgctg ggcatttctg ggcttgccgg aaggtatcgg ggggagggca
     9541 tcggaatatg cggcgcaccg ttcaaaccgg ggcggcgttt ccctattttc agggggttgc
     9601 aggtttcaac aaattgaaag atattttttg catttccgac cgtatgctga ttttgaagcg
     9661 ggataaatta taggacaagg tttttcgccc gccgcaaagg ttggccgcaa tgccgtctga
     9721 aaacaacggc ttgccgctaa ggcggcggta tgcggatttg cgctgccgaa atcggtttgc
     9781 catacccggc ggtgcaatct gcggggaggg ataagccgat aaggcgggac ttatcctgtt
     9841 ttttgtttat aatgcaacag aatttggacg aaccgtcggg aatttatcat gatgaaaaag
     9901 atactggccg tatcggcact atgcctgatg actgcggcgg cacgggctgc cgatacttac
     9961 ggctatctcg ccgtttggca gaatccgcag aatgcggacg atgttttgca ggttaaaact
    10021 acaaaagaag attcgacgaa aagcgaagcg tttgccgagt tggaagcctt ttgcaaaggt
    10081 caggacacgc ttgcgggcat tgccgaagac gagccgaccg gatgccggtc ggtcgtgtcg
    10141 ctgaacaata cctgtgtcgc gctggcatac ccgaaagcct tgggcgtgat gcgcgttgaa
    10201 aacgccgtcg tgattacttc tccgcgtttt acgagcgttc atcaggtcgc actcaaccag
    10261 tgcatcaaaa aatacggcgc acagggacaa tgcggcttgg aaacagtgta ttgcacgtct
    10321 tcttcttatt acggcggaac tgtgcgctct ttgattcaaa atctcaaata aaacggaaaa
    10381 tgccgtctga aagatgttca gacggcattt ctatatcgac ggtcaggatt ctttcggatc
    10441 gggcagcagg ctgttcaaca taatggcaag tacggcgcac aagcccacgc cggcaaagct
    10501 gaagctgccc aatttgagcg tcatgccgcc gatgcccgtg gtcagtaccg agctgacgat
    10561 gaccaggttt ttcggcagca tcaaatcgac tttggcatca atcagcgttt tcacgcccaa
    10621 agaagcaatc gtgccgaaca gcagcagcat aatgccgccc attactggca tcggaatgga
    10681 agccaaaaac gcattgaatt tgccgaaaaa cgccatgcag acggcaaaaa ccgccgccca
    10741 agtcatgatg acggggttgc tgtttttggt aatcattacc gcacccgtta cttcgccgta
    10801 ggtcgtaacc ggcgggccgc cgatcagacc cgcaacgcat acgcccaaac cgtcgcctgc
    10861 aagggttttg tccaagcccg ggtctttcgt atagtctttc cccgtcacat tgccgattgc
    10921 catgatgccg ccgatgtgtt cgatggcggg ggcgacggca acgggcagca taaacagtgc
    10981 agcctgccag ttgacctgag gcgtttcaaa atggggaacg gcgaaccagg gcgcgtgtgc
    11041 aatgcttgcc gtgtccacca gtcccatcag cagtgccaaa acataacccg aagcgacacc
    11101 gatcaagata ggaatcagct tcatcatcct gctgccgaaa accgatacga tggcggtaac
    11161 ggcaaaggta aagccggaaa gaatcagcga atcggtatag tcgatgacct gtttgccgtc
    11221 cgcctgaccc attgccatgc tgcttgccgc cacggcaaca gacagaccga tgaccatgat
    11281 gacggggccg atgacgaccg gaggcagcag tttgtgtacc gctgccagtc cgcgccaacg
    11341 gatcagcgcg gcaaacacaa aatacataaa gccggcggca aacagtccga acatggtgga
    11401 aggcagcccc cattcgccga cggagtagat aatcggtgcg ataaaggcaa acgaagaacc
    11461 aagaaaaatc ggcactttgc gtttggttgt gatttggaac agcagcgttc ccaagcctgc
    11521 gcccaaaagc gcaagagccg gattcagacc ggtcagcagg ggaaccagca ccattgcgcc
    11581 gaatgccaca aataaaatct gtgcaccgga aacggcaagt ttcagttggt ttataccatc
    11641 ttctttcaat cggttcaaac ggcacggatt atactggttt gccgagggtc tgcgtaaaaa
    11701 tacatatggc gggcaacaga tatgccgtct gaaaggcaat gtctgtctgt tgcaggatga
    11761 aagcaggaga aatggagtat aatccgacac tttccatatt caatccgcaa gccgtccggc
    11821 ttccttattt cagacggcat tttccggtaa tacggcatga cagaaaaatc ccataaaaaa
    11881 acagcaaaag gcagggcggg aagcccgtcc ccaacgtccg cacgcaacaa gaaagccgac
    11941 aacggcgcac ggggcaataa agtttccgag cggctcaagg cggtcaaaga gttgcagaaa
    12001 accgaaacca aaaaggcgcg tcccgaacat gtcgtcaacc ttatcggcga cgcactgtgg
    12061 ctgatgggtt tggcggcaac cctgtatttg gcgatttccc tgatcagttt cgatatgggc
    12121 gatccgtctt ggtcgcacag ttcgccggtt gtggaagatg tcgccaattg gggcggactg
    12181 ttcggcgcgt atgttgccga tgtcggctat tatcttttcg gctggtcgtt ctggtggtgg
    12241 atagcggctg cctgcgtcat gctgtataaa aattttcgtc tgcacgcaaa acagacggaa
    12301 aacgaggcat acaaccacaa aatcgctgcc gccgcgctgt ttgtcctgac ggtcttcagc
    12361 cccgtcttgg agtattttgt gctgggcgga aaatatgccg actccctgcc tgtcggagca
    12421 ggcggtatgg tcggcatacg cgtcggcgca gtgtttgcgt ggctgctggg gaaatcgggc
    12481 agcctgctga ttatcttggt tgttctgctg ttgtcgttgt ccctgctggt gcagatttca
    12541 tggctggaat ttttgaacgg tgcgggcagg gcggttcaaa accgcctgag tgccttatcc
    12601 ggcaaggtca tggctttagg aaaacgccgg ccgaatagca aaacagacgg tgtcgatacc
    12661 caaaatacac ggcgcatggt aaaagaagcc aagaatatta cggccaaacc cgttgccttg
    12721 cccgaaggca gcagcagcaa ccgcaaatcc gtcgcggttt ccgtcgcgcc gtcgcccaaa
    12781 attcaggttt ctctgtttga agatgacgaa cctcggcagg cgggcgaata ccacaagcct
    12841 acattgaacc tattgcggat tcctgacagc gaacccgtca gcatcaatcc cgccgaattg
    12901 gagcgcactg ccgaactgat cgaatccaaa ctggcagaat tcggcatcgg cgtacaagtc
    12961 gtatccgcca catccggtcc cgtcatcacg cgctacgaaa tcgaacccgc gcaaggtgtt
    13021 aaaggcagcc aaattgttgc cttgtcgaaa gatttggcac gctctatgtc gctgcaatcc
    13081 gtgcgtatcg tcgaaaccat cgcaggtaaa aacacgatgg gcatcgagtt gcccaacgac
    13141 aaacgccaag acgtgatgtt gagcgaaatc ttgtcctcgc ccgtgtttgc cgaagccaaa
    13201 tccaagctga ccgtcgcgct gggcaaagac attgccggta cccccgttgt cggcgacttg
    13261 gcgaaaatgc cgcacctttt ggtcgccggt atgactggtt cgggcaagtc cgtcggcgtg
    13321 aacggcatga ttatgtctat gcttttcaaa gctacgcccg acgaagtccg ctttatcatg
    13381 atagacccga aaatgcttga gttgagcatt tacgacggca ttccgcacct gctctgtccc
    13441 gtcgtgaccg atatgcgcga agcggggcag gcgttgaact ggtgcgtcgc cgaaatggaa
    13501 aaacgctacc gcctgctttc ccacgccggt gtacgcaatc tggaaggctt caaccaaaaa
    13561 gtcgaagccg caaaagcggc aggcaagccg ctgctcaatc cgttcagtct gaaccccgac
    13621 agccccgagc ctttggaaaa actgccgatg attgtggtcg ttatcgacga acttgccgac
    13681 ctgatgatga ccgaacgcaa agccgtcgag cagcaaatcg cccgtctcgc ccaaaaagcg
    13741 cgcgccgccg gtatccatat gattgtcgcc acccaacgtc ccagtgtcga tgtcgttact
    13801 ggcctgatta aagccaacat cccgacgcgt atggcgttta ccgtgcaaag caaaatcgac
    13861 agccgtacca tcctcgacca aatgggcgcg gacgaactgc tcaaatatgg cgattcgctg
    13921 ttcctccagc ccggcagtgc cgaaccgacc cgcctgcaag gcgcgtttgt ttcagacgac
    13981 gaagtacatc aagtcgtcaa ctacgtcaaa tcgcaagccc cagccgacta tattgaaggt
    14041 ctgctcagcg gcgaggccgc gctggaaact gccaatatcg ttaatccgaa tgcagacagc
    14101 gacgaattgt tcgatcaggc agtcgcctat gttttggaaa gcaaaaaaac ctccatttcg
    14161 tctttgcagc ggcagttgcg tatcggctat aaccgcgcgg caaacctgat ggaggcactg
    14221 gaaaatgcgg gtgtcgtttc tcccaccgac ctcaacggca gccgtaaaat tttggcgcac
    14281 aaggaccatt tgtagcccgt attgcaaaat gccgtctgaa cggcggaatt ggcgtttcag
    14341 acggcatatt atgtttcagg cggaacattg tgatatactt gccagctaaa atctcccctt
    14401 tgcggcaatg cggttcaaat atccgtaccg ttgcgctgtt tgcttcccca tgcagggaag
    14461 aaggtttacc attttatcaa cacaacaaat ttaagggctt atgatgagcg taactgttga
    14521 aactttagaa aatctggaac gcaaagtagt gttgtccctg ccttggtccg aaatcaacgc
    14581 agaaaccgat aaaaaactga aacaaaccca gcgccgtgca aaaatcgacg gtttccgccc
    14641 gggtaaagca cctttaaaaa tgattgccca aatgtacggt gcgagcgcgc aaaacgacgt
    14701 gatcaacgag ctggtgcaac gccgcttcca cgatgttgcc gttgcccaag agttgaaagt
    14761 ggcaggcttc ccccgttttg aaggcgttga agaacaagac gataaagaat ctttcaaagt
    14821 tgccgccatt tttgaagtgt tccccgaagt cgttatcggc gatttgtctg cacaagaagt
    14881 tgaaaaagta accgcttccg tcggtgatgc cgaagtggac caaaccgtag aaatcctgcg
    14941 caaacaacgc acccgcttca accatgtcga acgcgaagcc cgaaacggcg accgcgtcat
    15001 cattgacttt gaaggcaaaa tcgacggcga accttttgca ggcggcgcat ccaaaaacta
    15061 cgccttcgta ttgggcgcag gtcaaatgct gcctgaattt gaagccggcg tagtcggcat
    15121 gaaggctggc gaaagtaaag acgttaccgt caacttccct gaagaatacc acggcaaaga
    15181 cgttgccggt aaaactgccg tgttcaccat tacgctgaac aacgtttccg aagcgactct
    15241 gcctgaagtc gatgcagatt ttgcaaaagc cttgggtatt gcggatggcg acgttgccaa
    15301 aatgcgcgaa gaagtgcaga aaaacgtaag ccgcgaagtg gaacgccgcg taaacgaaca
    15361 aaccaaagaa tccgtaatga acgcgctgct caaagccgta gagctgaaag cacctgttgc
    15421 tttggtcaat gaagaagccg cacgcttggc aaacgaaatg aaacaaaatt ttgttaacca
    15481 aggtatggct gatgctgcca acttggatct gcctttggat atattcaaag aacaagccga
    15541 acgccgcgtg tctttgggtc tgattttggc caaactggtt gatgaaaaca aactggaacc
    15601 gactgaggag caagtcaaag ccgttgtcgc caacttcgca gaaagctacg aagatcctca
    15661 agaagtaatc gactggtact acgcagagcc ttcccgcctg caagccccga cttctttggc
    15721 gatagaaagc aacgtcgttg atttcgtttt gggtaaagcc aaagtaaacg aaaaagcttt
    15781 gtcttttgac gaagtgatgg gtgcgcaagc ctgattatcc tgaaaatgcc gtcttagggc
    15841 agtttttgaa agcaccgaag ccgcttttaa agcccgcttt ggtgctttta catcatgaaa
    15901 ggagacgaaa tgtcttttga taactatctt gtccctaccg ttatcgagca gagcggtcgc
    15961 ggtgagcgtg cattcgatat ctattcccgg cttttgaaag agcgcatcgt attcttggtc
    16021 ggtccggtaa ccgacgagtc cgccaatctg gtggttgccc aactgttgtt tttggaaagt
    16081 gagaatccgg ataaggatat tttcttctat attaactcgc cgggcggttc ggtaacggcc
    16141 ggtatgtcga tttacgacac catgaatttt atcaagcccg atgtatcgac tttgtgcttg
    16201 gggcaggcag caagtatggg cgcgttctta ttgtcggcag gcgaaaaagg caaacgtttc
    16261 gccctgccca acagccggat tatgattcac cagcctttaa tcagcggcgg cttgggcggt
    16321 caggcatccg atattgaaat tcacgcacgc gaactgttga aaatcaaaga aaaactcaac
    16381 cgcctgatgg cgaaacattg cggccgcgat ttggcagatt tggagcgcga caccgaccgt
    16441 gataatttca tgtccgccga agaggctaaa gaatatggtc tgattgatca aatcttggag
    16501 aaccgtgctt ctttacagtt ttaataagaa atctgagaga aacaaatgcc gtttgaaaac
    16561 agttcaggac ggcatttgtc ttatagggac tgtataggga tgattttgtt ctggttgcgt
    16621 ttggagccgt tttcgccata attggtttta acatattcct ctacaatagc taggaattca
    16681 gttgcaatat tatcaccttt cagtgtgact ttgcgctccc cgtccacata aaccggcgcg
    16741 acgggtgttt ctcccgtacc gggcaggctg atgccgatgt cggccaattt gctttctccg
    16801 ggcccattga caacgcagcc catcacggca acattcaggg attccacgcc tggataagca
    16861 gtacgccata cgctcatttt ttgtcgcagg taattttgaa catcttgtgc cagctcttga
    16921 aatacggtac tggtggtacg cccgcagccg gggcaggcgg taaccatcgg cgtaaacgaa
    16981 cgcaatccca tagtctgtaa aatctcttgc ccgacgacga cctcctgagt acgcgggctg
    17041 ccaggttccg gagtcagtga aatgcggatg gtgtcgccga ttccttcttg aagcaagacg
    17101 gataatgccg ccgttgatgc gacaatgcct ttgctgccca taccggcttc ggtcaaaccc
    17161 aaatgcagcg gataggcgca acggctgccc agttcgcggt aaacctgaat caaatcctga
    17221 accgcgctga ctttgcacga caggatgatt ttgtcttcgg gcagtcccaa tagaacggct
    17281 ttttcggcag attccaaagc ggagacaatc agtgcttcct tcgtcacttc ttcgggcggt
    17341 ttcggcgcgg aagaagcgag gttggcatcc atcatacgtt tggcgaggct ttgatccaaa
    17401 gaaccccagt ttacgccgat gcggacggct ttatcgtttt cagcagcagt ccgaatcata
    17461 aaggcaaatt tttcatcgcc ttttacgcct ttgccgacat tgccgggatt gatgcggtat
    17521 ttggacaatg ctttgccgca ttctggaaat tccgccaaca ggcgttcgcc gttgaagtgg
    17581 aaatcgccga taagcggtgt ggcatagccc atatcgtcca agcggcggcg gatttcggca
    17641 actttggacg cggcttcggg gctgttgacg gtaatacgca ccatttcgga tccggcatcg
    17701 ctcaattcct taatctgcaa tgcggtggct tttgcatcgg cagtgtcggt gttggtcata
    17761 gattggataa cgacgggtgc ttctgaaccg acggtaatat gatcgatgcg gacttgatgc
    17821 gtcttgcggc gttggagtgt gttcatatgg tttgaatctg tttattgacc ggtgaggacg
    17881 gtttgcaatt cttccgagta ggggaaattc gcctgcaatt gtgcttcata ttcgtatgcc
    17941 gcctgtgcgt tgccgagggc tttggcaatt ttccagccta gcagcaaatc atcggcctga
    18001 aggacttcta ccctgctttg gtatttttta aagtagtaat cggcatcgcc caactgcccg
    18061 gccagcattt tggtgcgcgc cagttcttta aatgcgggtg ggaactgcgg ctgggcggcg
    18121 agggaacgtt tcaaataggc ttccgccaat ccgaattgcc cctgttttgc gctgcatatg
    18181 cctttattca ggttggcaat ataaggggtc gggtaggtgg ggtcggccag ggctttgtcg
    18241 aaatatgcca tagattcggc agggcggttg agcctgccgc acaggaacca accgtagttg
    18301 ttgttgattt cggcactgtc gggtttgatg gagagggctt gccggaaact tttctgcgcc
    18361 ttgtcgttaa ctttcaggta ttggtagatt tcggcacgga ccagccaggc aagctcgttt
    18421 ttagggtctg atttcagggc gtcttcaata cttgccgtcg cctgacggta gtcctgaccg
    18481 cgcatatatt ccattgccaa ctgggttttg atattggaaa cctgattggc tttttctgcc
    18541 cgcgaggggc ggtaggaagt gctgcacgcg cccaaggcaa ggacaagtaa taaagagatt
    18601 cgtttggatg gcttaaaagg cataattacc cctgttgtcc gattaaaatc tgctgccatt
    18661 tttgttggcg gcgcgtttta tcctgaacct gccccgccaa ctgtccgcag gcggcatcga
    18721 tgtcgtcgcc gcgcgttttt cgtacggtaa cgacaaatcc tgcctgctgc aaaatatcgc
    18781 ggaacacacg gatgttctca ttgctggagc gttcgtatcc ggagtttggg aagggattga
    18841 acggaatcag attgaacttg cagggaacat ctgtgaccag ttcgatcagt tcgcgcgcat
    18901 gttgcgcctt atcgtttatt ccgtccaaca tgacgtattc gaaagtgatg aaatccctgg
    18961 gtgctttgac cagatagcgt tggcatgcgg ccatcaattc tttcaaggga tattttttgt
    19021 tcaacggtac gatttggttg cggacttcgt cattggaagc gtggagggaa accgccaaag
    19081 ccaccggcat gacatcgcgc aacctgtcca tttggggaac catacccgaa gtggaaacgg
    19141 ttacgcggcg gcggctcaaa ccgtagccgt ggtcgtccag catgatgctt aaggcggtaa
    19201 cgacattgtc gaagttcgcc atcggctcgc ccatgcccat catgacgacg ttggaaatca
    19261 cgcgctcgtt tttcggtgta acgcccatcg ctttgtttgc ccaccacaat tgcccgatga
    19321 tttcggcagc agtcaaattg cggttgaagc cctgccggcc ggtcgaacaa aatgtacatt
    19381 ccaaagcgca gccgacttgt gaggaaatgc agagcgtgcc gcgatccgat tcggggatga
    19441 agacggtttc caccccgttg cccgtaccga catccaaaag ccattttcga gtgccgtctg
    19501 aagatttttg agacatcatc agcttgggaa tttcgatgcc tgcctgttcg ttcagtttat
    19561 ggcgcaacga ttttgccaaa tcggtcattt cgtcaaaatt ttgcgcgccg gattggtgca
    19621 tccaacgcat aacctgtttg gcacggaaag gtttttcgcc catatcggca aaatgtcggg
    19681 tcagcccttg gaggtcgtag ttgagcagat tggttttcat gtatttgtgt ttttcttaaa
    19741 ttaaggcttc aaataaaaaa ttgcagggca ggcaagtttg atttgaagcc ttttgcaaca
    19801 gagttttcag acggcatgga tatattttat gccgtctgaa gaggagttgt gcggtgtatt
    19861 gtatcaacgg gggcagattt cggtttggct gaaaaagtag gcaatttcca aagcggcatt
    19921 ttccggagtg tccgagccgt gtacggcatt gatgctgacc gaagtggcaa agtccgcgcg
    19981 tatcgtgcct tcggcggctt cggcgggatt ggtcgccccc atcagttcgc ggtttttcag
    20041 aacggcgttt tcgccttcca gtacttgaat cataaccgga ccgccggtca taaattcaac
    20101 caatccggcg tagaaggggc ggtctttatg aaccgcataa aattcttgcg cctctttgag
    20161 ggtaagctgc ttcattttgg cggcaacgat tttcagaccg ttctcctcaa agcggctgta
    20221 tattttgccg atgacgtttt tgccgacggc atcgggtttg atgatggaga tggtacgttc
    20281 aatcgccatg ctatatcctt atttttactt aagaagaatc aaatcggtat tctatcaaaa
    20341 aaataactta tccgcctgaa tccttgggag atagggtcag gtgcggctgt tccaaataaa
    20401 cttcggactg catttcgacc atccggctgg cggtgcgggt aaattcttcg gcaaaatcgc
    20461 cttccgtata gatatggttg acatctaccg cgccggtggc acacagtttg acccggaaat
    20521 cgtagagtac gtcaatcagc caagtcagcc gccgcgcctc cgccttttct tgcggtgaga
    20581 gttgttccaa acctgaaata aaaaccattt cataatgttc ggccaaatac agatagtcgg
    20641 actgtgagcg ggggccgaag cacagtgcgc ggaaatcaaa ccatatggca cggccggact
    20701 cggctttgtg gggaatctcc cgaccgtgga tggtgctgat gccggggttc aaatcggtaa
    20761 tgcctgtcat ttctttgaac agttttgcca gttttgcctc attttcttca ttggcaggcg
    20821 taaagaaaat ctcggcgggg cggagggtac gcagtcggta gtcttcaccg ccgtcaacgt
    20881 ttaagacggt caggctggac tcgatgagcg cgattgtggg aagaaaactg ctccggtttt
    20941 gaccttgcgg gtagagttcg gaaggcgcgt agtttgaagt cgccaccaaa acaacgccct
    21001 cgttaagcag gttttccagc agacggccta aaatcattgc atccgcaata tcgctgacat
    21061 gaaattcgtc aaaacataat acgcgggttt ctttggcaat ctcggcggca acggatttca
    21121 acgggttgct ttcgcttttc agggttttca gcctctggtg gatttctgcc ataaaggcat
    21181 gaaagtggac gcggcgtttg cggcggtaag ggaggcagcc gaaaaaagcg tccatcagaa
    21241 agcttttgcc gcgtccgacc ccgccataga aataaagccc tttggggact tgcggggaac
    21301 gcaaactcct gcctaaaaaa cggtttcttt tgcgtttgaa catcatcaat tcggtccaaa
    21361 gccgatcgag gtgttcgatg gcggctgcct gcgcgtcgtc gcggatgaag ttgggcagtt
    21421 gtgaggcagc ctgataccag gtcagcgggc tgtggttttc aaacggcggg gctttaaaaa
    21481 gttggtctct attactcata tttaaccttg ttatacttct tgcggcggag aaagaaaatg
    21541 cttaccgtcg gtattataaa tgatttcctt caaacttggc attttccgtg gaaaagaata
    21601 atgacgcgca caagggggta taagcaaagg cggcgtttca atatggtgca gatatgagct
    21661 caaatgcctt ttatagtaga ttaaatttaa accagtacag cgttgcctcg ccttgccgta
    21721 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataatc
    21781 cgtagagtcg gctccttccg gctcagacgg catacgttta tgccggcact tcttaccgcc
    21841 ctcgtgccgg ctatcccaaa actgctttga acactgaacg ctctttggtt tacttcaaaa
    21901 acgcggcgtg ataagcaata tgttcgccaa taaaactggc gatgaagtaa taactatggt
    21961 cgtagccttt gtggaagcgc acatcgaccg gctggtttgc cgcacgacag gtttcgataa
    22021 aatcttcggt acgcaattgt gtcggcaaaa actcatcttc caagccctga tcaatgcgta
    22081 taccttgcac tttatagcct tgttgaatga gcgagctggc atcatattgc tgccattttt
    22141 cacgatcttc ccctaaataa gcagtaaagg ccttttctcc ccaaggcacg aggcttggcg
    22201 ataaaatagg cgaaaaagca gaaacgcttt gataacgttt ccgatttcgc agtgccaata
    22261 ccaatgcacc gtgtccgccc attgaatgcc ccataatgga acgtttgccg ttagtagaga
    22321 aatttgcttc aaccaaatca ggcaattcgt tcaagatata atcatacatt tgataattcg
    22381 tcgcccaagg ctgctcagtt gcattaagat aaaagcccgc accttgtcct aaatcgtaag
    22441 ccgcatcgtt cggcacttgc tctccgcgag ggctggtatc aggggcaacc acaatcactt
    22501 gatgttctgc tgcataaagc tgaaagcctg atttagtaat gaaattttgt tccgtacacg
    22561 tcaagccgga aagccaataa atcacaccaa gcggttgatt ttccgaatta tttggcaaat
    22621 agacggcaaa tttcatctcg cattgaagcg tttgggcatc atgagcccaa acttgctgca
    22681 aaccgccaaa aatttgatgt tgttcaatca gtttcatcgc ataccttagt agtgaataac
    22741 ggcgcggatc gatttacctt cgtgcattaa gtcaaaggct ttattgattt gatcgagtgt
    22801 cattgtgtgg gttacaaacg gttctaactc aatgtcgcct ttcattgaat cttccaccat
    22861 tttcggcagt tcagagcgac ctttcacacc gccaaatgct gaacctttcc acacacggcc
    22921 tgttaccaac tggaacggac gcgttgaaat ttcttgtcct gcacctgcta cgccgatgat
    22981 aatggattga ccccaaccac gatgtgcact ttctaatgct tgacgcatta cgtttacatt
    23041 gccgatacat tcaaaggtat ggtcaatgcc ccatttgttg atgtctaaca acacatcttt
    23101 aattggttta tcgtaatcgt tcgggttcaa acaatccgtt gcaccgaact gttttgccaa
    23161 ctcaaatttt gatggattgg tatcaatggc gataatgcgg ccggctttgg cttgacgcgc
    23221 accttgcacc accgccaaac caatcgcgcc taagccaaac acggcaacag agtcgccttc
    23281 ttgcactttt gccgtattat gtaccgcacc aatacctgtg gtaacgccgc agccgagcaa
    23341 acatacttgt tcatggttgg cttcagggtt gattttcgcc agtgaaactt cggcaacaac
    23401 ggagtattca ctgaaagtcg aacagcccat atagtgataa atcggctgac cttgataaga
    23461 aaaacgcgtc gtgccgtccg gcattaagcc tttaccttgt gtatcacgca ctgagacgca
    23521 caagttggtt ttacctgaac aacaaaactc acattcgcca cattcggcgg tgtaaagcgg
    23581 aatcacgtga tcacccggtt ttacgcttga cacaccttcg cccacagcaa cgaccacacc
    23641 cgcaccttcg tgtccaagca ccacagggaa tacgccttca ggatcgcttc ctgataatgt
    23701 aaacgcatca gtatggcaca cgccagtgtg ggtattgcgg attaacacct cgcctttacg
    23761 cggcatttct acgtcgattt ccacaatttg taagggttgg ttaggggcga atgccaccgc
    23821 cgcacgagat ttgatgcttg aatcggtttg tttcatttcc attgtctttc ctcttaattc
    23881 attgacaaag ttgccttacc tgagcaacat agcaacagtt tacactttag agttaactct
    23941 aaggcaagtg ttttttactg
//
LOCUS       DAXIHS010000024        22976 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXIHS010000024 DAXIHS010000000
VERSION     DAXIHS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22976
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.024"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            127..678
                     /locus_tag="ACFOF2_000842"
     CDS             127..678
                     /locus_tag="ACFOF2_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107193.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(955..1646)
                     /locus_tag="ACFOF2_000843"
                     /pseudo
     CDS             complement(955..1646)
                     /locus_tag="ACFOF2_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1952..2722)
                     /locus_tag="ACFOF2_000844"
     CDS             complement(1952..2722)
                     /locus_tag="ACFOF2_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107194.1"
                     /translation="MASIALYRLSFPVAAGGSGSRCTLCTKVHRESMLAIYSINILIY
                     NEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKHKCLIDFILTVASQIAYADLPLSLEEL
                     LTDKGKFKLESSISYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQV
                     FDSQNVKAIQLSQAEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGA
                     ATAAGIGAATGVAAKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2753..2911)
                     /locus_tag="ACFOF2_000845"
     CDS             complement(2753..2911)
                     /locus_tag="ACFOF2_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107195.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2908..3750)
                     /locus_tag="ACFOF2_000846"
     CDS             complement(2908..3750)
                     /locus_tag="ACFOF2_000846"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC7107196.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3988..4908
                     /gene="pyrB"
                     /locus_tag="ACFOF2_000847"
     CDS             3988..4908
                     /gene="pyrB"
                     /locus_tag="ACFOF2_000847"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC7107197.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4918..5376
                     /gene="pyrI"
                     /locus_tag="ACFOF2_000848"
     CDS             4918..5376
                     /gene="pyrI"
                     /locus_tag="ACFOF2_000848"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC7107198.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5441..6106
                     /locus_tag="ACFOF2_000849"
     CDS             5441..6106
                     /locus_tag="ACFOF2_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107199.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6169..7386)
                     /locus_tag="ACFOF2_000850"
     CDS             complement(6169..7386)
                     /locus_tag="ACFOF2_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC7107200.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAGE
                     RLKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            7570..8073
                     /gene="def"
                     /locus_tag="ACFOF2_000851"
     CDS             7570..8073
                     /gene="def"
                     /locus_tag="ACFOF2_000851"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC7107201.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            8160..9086
                     /gene="fmt"
                     /locus_tag="ACFOF2_000852"
     CDS             8160..9086
                     /gene="fmt"
                     /locus_tag="ACFOF2_000852"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC7107202.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9165..10424
                     /gene="rsmB"
                     /locus_tag="ACFOF2_000853"
     CDS             9165..10424
                     /gene="rsmB"
                     /locus_tag="ACFOF2_000853"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC7107203.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10390..10989
                     /locus_tag="ACFOF2_000854"
     CDS             10390..10989
                     /locus_tag="ACFOF2_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC7107204.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10989..13109
                     /locus_tag="ACFOF2_000855"
     CDS             10989..13109
                     /locus_tag="ACFOF2_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC7107205.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGLPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSIDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELADEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            13102..14379
                     /locus_tag="ACFOF2_000856"
     CDS             13102..14379
                     /locus_tag="ACFOF2_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC7107206.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14470..15657
                     /gene="dprA"
                     /locus_tag="ACFOF2_000857"
     CDS             14470..15657
                     /gene="dprA"
                     /locus_tag="ACFOF2_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC7107207.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGEKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15683..16144
                     /locus_tag="ACFOF2_000858"
     CDS             15683..16144
                     /locus_tag="ACFOF2_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC7107208.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16223..18529
                     /gene="topA"
                     /locus_tag="ACFOF2_000859"
     CDS             16223..18529
                     /gene="topA"
                     /locus_tag="ACFOF2_000859"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC7107209.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18602..19297)
                     /locus_tag="ACFOF2_000860"
     CDS             complement(18602..19297)
                     /locus_tag="ACFOF2_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107210.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(19317..19868)
                     /locus_tag="ACFOF2_000861"
     CDS             complement(19317..19868)
                     /locus_tag="ACFOF2_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107211.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19987..20364
                     /locus_tag="ACFOF2_000862"
     CDS             19987..20364
                     /locus_tag="ACFOF2_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC7107212.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            20453..21022
                     /gene="rsmD"
                     /locus_tag="ACFOF2_000863"
     CDS             20453..21022
                     /gene="rsmD"
                     /locus_tag="ACFOF2_000863"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC7107213.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21145..21396
                     /locus_tag="ACFOF2_000864"
     CDS             21145..21396
                     /locus_tag="ACFOF2_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC7107214.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21493..21576
                     /locus_tag="ACFOF2_000865"
     tRNA            21493..21576
                     /locus_tag="ACFOF2_000865"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21527..21529,aa:Tyr,seq:gta)
     gene            21607..21680
                     /locus_tag="ACFOF2_000866"
     tRNA            21607..21680
                     /locus_tag="ACFOF2_000866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21639..21641,aa:Gly,seq:tcc)
     gene            21690..21764
                     /locus_tag="ACFOF2_000867"
     tRNA            21690..21764
                     /locus_tag="ACFOF2_000867"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21722..21724,aa:Thr,seq:ggt)
     gene            21813..>22976
                     /gene="tuf"
                     /locus_tag="ACFOF2_000868"
     CDS             21813..>22976
                     /gene="tuf"
                     /locus_tag="ACFOF2_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC7107215.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT         5695 a         5824 c         6319 g         5138 t
ORIGIN      
        1 gacgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
       61 aagtttgtcc tagttatcta ggacagcccc ttattttttg ttcggcggct tgcgtggtcg
      121 ggtaaaatga aggttttgaa cggttggtcg gacaggaaga tgtggcgggt tttgagtgct
      181 ttgccgatag gcgtggtgtt ttttgatttg atctacggtt ttgtgttgaa tgtgttgcag
      241 ggtttggatt tgcagcgtgc cgtgccggat ttggaaggcg tgttggcggt tacgcccgat
      301 attgcattca acagtttgca gattgtcgcc aacggcggta tggcggcggt ggtctgtttc
      361 gggttggcgg ttgtgttttt gctcaaccgt tcggtgcggc ggcggcaggt gttggaaatc
      421 ggggtgttcc ggatgttggg gctggtggcg gtattggcgt tcagcgcgcc gtcggtgtgg
      481 gagtgggcga acgcgctacc gctgctgctg aagggcgcgg acgtggtcaa tacggggaat
      541 gcgcgttatg tgctgacggc tttgtgtatg ccctttccgg cggtgtcgtg cgtcatcggg
      601 ctggtggggc ggttcaggct tcagacggca tcgggcaggg cggcaaagtc agggggtgcg
      661 ggcaaggcgg acggatagga cgcatttttc agcgggtgcg tcgagaagca gccgatgtgt
      721 ttggcagccg cagcttgggg gggtgtagtg ctaatggcgg tttctttgct tttatagtgg
      781 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg
      841 tgctgaagca ccaagtgaat cggttccgta ctattggtac tgtctgcggc ttcgtcgcct
      901 tgtcctgatt tttgttaatc cactatataa aataaatggg caaaaatcgg tttattatcg
      961 tttttgccgc atttggattt gttctaccgt aaaacgtgtt tgacgaacgg gattcttatt
     1021 aaaaaacatc tgatttctaa caaaatcagt attttttggc acgatggcta aaatttttcc
     1081 ttccatttcg ccatcacgtg ttttccatgc gctcaagaat tgtgatttgc tcattgagac
     1141 gtgccccagc gatggatcag ccagcaaaac agtttctccg ttaataccgt tcaataccga
     1201 aaaatggttg tttttacggt attttaaata cacaattaca ggaattttta gttgtaccaa
     1261 ctgttcaaat ggcaaagcat aaccttgtgc ttcaaaaccc agttcgggca ttatgcgttg
     1321 catatcatca aaagaagcac gcatttgggt tttatccatt ttgtccaaga tttccgcttc
     1381 agaataatgt ctgccataaa aattattcag taacgtggca atcgaagccg cgccgcaaga
     1441 aaaatccaaa tcttgtttta ctatgccgga atctcgccgt gctttccaac ttcgtacatg
     1501 gatgttttgg taagaagcgg ggggtcaaca aacataggcc aagcaaaaac tatatttggg
     1561 gcgaaaccaa tcaaagccgc ataatttatc aatttataaa gattttttat cataatatgt
     1621 atacgcggaa taaacgcatg aaacataaaa aaataaaatc atatgcaatt ttattgcatg
     1681 aataaatatg aataaaatag ataatgatgg gagtaaatac gccatgtatt ttggaagttt
     1741 aaatttatta ataataaaat aatttatcgt agcgcaaata aatcccaaaa ttggaacgat
     1801 tagaaaaaaa attaatacac ccatcatcat gcaaactcta tattaataaa tagctaacta
     1861 attctattgt ggaaattagt tagctaacta aaagttatta atgattattt ccgagaattg
     1921 actgcattgt tggcagcatt ggccccaaaa cctagtgcat gaatacccgg tctccatgcc
     1981 aaattcccag ccaatccgcc tccggcagca gcagccagcc ctgttttgcc gctactcctg
     2041 ttgccgcacc gattcctgcc gcagtagccg cgccttgcgc agttcctaat ttaccatgat
     2101 tatacaaatt agcaccatga tactcccatg cacctaatgc accgccaaaa gcagcggctg
     2161 caataatggg aacaaattca ccttgtgttt ctttcatttc agcctgtgat aattgaattg
     2221 ctttcacatt ttggctgtca aaaacttggc tgtctaaatt ttgcgccatt acaggtgtaa
     2281 tcatcatagc cattacagtt gcaattttcg ttgcgctggt ttgcacataa ataggattag
     2341 caaattcgct ttgattgcgt tcagtgttga tgtagctaat actgctttct agtttgaatt
     2401 tacccttgtc agtcaataat tcttccaaac ttaaaggtaa gtcagcataa gcaatttggc
     2461 tggcaacagt caaaataaaa tctattagac atttgtgttt ttgcatcatt tcgtttgatt
     2521 ttctagtttt tgagaatgat acaaagtttt ttacaaagta aagagtcact ctgaaaaaca
     2581 ttttttcatt ataaatcaaa atattgatag aataaatagc gagcatcgat tcacggtgta
     2641 ctttagtgca aagcgtacaa cgcgagcctg aaccaccagc cgcaacagga aaagaaagcc
     2701 gatagagtgc aatgcttgcc aacgtgcaag cgagcttgca agaacgcttg gctcaacgag
     2761 agcaggcaag acagaaagca gaaagcagga taggagcggt aacgcaaagg tctcgggctt
     2821 tgatttcgcc gtaaaccctg ctgccgcctt gtccggaaag ggcgcaggcg gcgagtgccg
     2881 acagggtgca gatggggagg ggggttttca tttggggtcg caacggaagt ggtatgcgca
     2941 gatttcaaaa ccgtttttga aatacaggcg gtgcgcgtcg gcacggtcgt ggttgacgtg
     3001 gacgttgagg tggattttgg ttacccctgt ttccgcgccg attttgcgga cttcttccaa
     3061 aaggcgcgag gcgtagcctt tgcggcggct ttgcggcagg gtaacgatgt catcgatgtg
     3121 gatgtggcgg ccgctggcga gggtgcaggc ttcgcggaag ccgcagacgg cgacggcatt
     3181 gtgtttgcct tcttcaaaaa tacccagcag gcggtagcct tgggggcgtt gggttttgtt
     3241 gatctgttcg gtaaagcggt tgatgtcggt cagggcggaa cgcaaaacgc tcaaggctgc
     3301 aaaggcggtg gcggtgtcgt ccgcgccgat ttcgcgcaaa acgtaggatg cgcccgaggc
     3361 ggtctgttcc tgtgctttct cggcggcgtg tttttcttcg attgcctgtg ccagcatgac
     3421 gtgttcgtcg gcagggttgt tttgtccgcc ctgttcgcgt tcttcgagca gggctttgca
     3481 gtcgatgacg cgcaggtcgt tgtcggcggc aaagtccatc aggaagcgga acatttgggg
     3541 gttgtcggtt tccagttttt tatcgacggc gacgcagcgg atgttgtcca ccagcatggg
     3601 gcgcacccat ttcgaatagg aaagcctgtg gtctttggtg aacgaggaca gaatgccgca
     3661 caggcgttcc gcccagtcgc tgggacggaa aatcttgccg gaactcgttg tgccgtggat
     3721 gacgacttcg taggggttgc agactaacat ggcggcttcc tgaaaagaaa tgtctggcgc
     3781 gattatacct tatgcttatg cgggcgtgtt tggatatgcc gtctgaaaag tacgggattc
     3841 gtgcggtaaa actttgcggc ggcaaatgtg cgataatacg cgccgtattg ccgcttttgc
     3901 gaagctgttc cgcaaacata cgggcggcgt ggacgacgta taaccggata cccgcctgac
     3961 gcgggttttt tacggaaggg ggcaaaaatg cctaatccgc tttacagaca gcacatcatc
     4021 tcgatttcgg atttgtcgcg cgaacagttg gaatgcctgc ttcagacggc attgaagctg
     4081 aaggcgcatc cgcgcggcga tttgttggaa ggcaaactta tcggttcgtg cttctttgaa
     4141 ccgtccacgc gcacgaggct gtcgtttgaa acggcggtgc agcggctggg cggcaaggtc
     4201 atcggctttt cggacggcgc gaataccagt gccaaaaaag gcgagacgct tgccgatacc
     4261 gcccgcatca tttccggcta caccgacgca atcatccaac gccaccccaa agacggcgcg
     4321 gcgcgcgtgg cagcggagtt ttcgcgcgtc cccgtcatca acgccggcga cggcaccaac
     4381 cagcacccca gccagacctt gttggatctg gtaacgattt atgaaacgca gggacgtttg
     4441 gacaagctca aaatcgccat ggcgggcgac ttgaaatacg gacgtaccgt gcattcgctt
     4501 tgtcaggcgt tgaaacgctg gggctgcgaa tttgcctttg tttcgccgcc cagcctcgcg
     4561 atgcccgact acattaccga agagttggac gaagccggct gccgataccg tatcctcggt
     4621 agtttggaag aagcggcgga atgggcggac atcctgtata tgacccgtgt tcaacgcgaa
     4681 cgtttcgacg aacaggaatt tgccaaaatc caaggcaaat tcaatctcga cgcatccatg
     4741 cttgcccgcg ccaaaccgaa cctgcgcgtg ctgcaccctt tgccgcgcgt ggacgaaatc
     4801 catcccgatg tcgatgctac gccgcatgcc tattatttcg agcaggcgac caacggcgtt
     4861 tatgcgcgta tggcgatatt gtcgctggtg ttgaacgaag aagtgtgagg aaccgatatg
     4921 gaaaccccga aactcagtgt cgaagccatt gaaaaaggta cggttatcga ccatattccc
     4981 gccggcaggg ggctgaccat cctgcgccag ttcaaacttt tgcactacgg caacgcggta
     5041 accgtgggct tcaacctgcc cagcaaaacc caaggcagca aagacatcat caaaatcaaa
     5101 ggcgtgtgct tggacgacaa agccgccgac cgcctcgccc tgttcgcccc cgaagcggtg
     5161 gtcaatacca tcgacaattt caaggtcgtg cagaagcggc atttgaacct gcccgatgaa
     5221 attgccgaag tgttccgttg cccgaacacg aattgcgccg gccacggcga gccggtcaaa
     5281 agccggtttt atgttaaaaa acacaacggg cagacgcggc tgaaatgcca ttactgcgaa
     5341 aaaacctaca gccgggattc ggtggcggaa gcctgacgga ttcccttaaa ccgagcgggc
     5401 ggcatttcgt ctgccgcttg ttttgccaat ctgaaatgga atgatgatgc acgcttctgt
     5461 ccaaagccgt ttcgcaccga tactttatgt tttgattttc tttgccggtt ttttgaccgc
     5521 gcaaatttgg ttcaatcaga aagcctatac tgaagagctg cctccgcttc tgtccgcatt
     5581 gtccgccgtc gcgctggtgt ggctggcgtg ggcgttcgtg tcggcgcgtt caaaggctaa
     5641 ggcggaaaag ttctaccgcg aaaaaatgat acagaacgaa agcatacacc ccgtcctgca
     5701 cgcttctttg caacacttgg aacacaagcc gcaaatgctc gccctgctgg tcaaaaacca
     5761 cggcaaaggg atggcggaac aggtcaggtt caaggcggaa gtgctgcccg acgacgaaga
     5821 cgcgcgcacg attgccgccg agttggcaaa aatggatatg tttgcattgg ggacggacgc
     5881 ggtcgcctcg ggcgaaacct atggacgcgt gttcgccgat attttcgagt tgtcggcggc
     5941 tttggaaggg cgcgcgttca aaggaatgtt gaaactgacg gcggaatata aaaacatctt
     6001 cggcgatgcc tgccgttcgg aaacggcgtt ggatttgggc gcgctcaatc aggcgttgca
     6061 ggagatttca aaaacatcgg aaaagtccaa acggatattt tattgaagat ggaaaaatgc
     6121 cgtctgaaac ggaaggtgtt tcagacggca tttttgtcgg atgattaatt attcggagcg
     6181 gtcgaagcca aacttcacgc ggctgcggcc ctgatccggt atattgtcca aatcgcgtcc
     6241 cggattggcg gcggtgtcgc ctacggaaat atcggagatg ttttccaaaa tgatggcgga
     6301 cgacaggtgt tcggaggtgc ggtaaaccat tgccaagccc acttcttcgg caggagtgga
     6361 aatcagctcg acggtatccc tgcttttgaa attgttggag aggtcgacct gcatcgtttt
     6421 cttgcgtttg tagaggctca aaaccgtgcc tttgtccaaa ccgtccgcct cgcctttgtc
     6481 gatggtgatg gtttgaaact ggccggcaat ccttgtgcct tcaaacacgg aaacgatttt
     6541 agcctgaacc gggcgggacg gttcgtgcgg catcatgttg aagcggtcgg tgtcttccgg
     6601 cattttcatc aggtagtcgc cctgctgtat ttcggaaatg gcggtttcga ccaccagcgg
     6661 ctgtatcgaa ggggtgcgca gcggggtaat caaaggatgg gtgcgggtat ggtattcgtt
     6721 gtctttcagc cgttctccag cctgtttcga gcgttgttcg agggcggagt cggtatagtc
     6781 gagggagcgc acgatgccgc tgaatgcgac ttcctgcccg aggaatttac ccgtatccgg
     6841 atcggtgatg tttttattga ttcggtaggt caggtagcgg cccggctctt tcaggccttt
     6901 ggtgtaaacc ctggtgcctt tggtgtacag cagcctgcct tccgggcccg agagcaggcg
     6961 cggcgcggca gcggtttctt tgcgggaaac gatttgcgga tgccgcataa agatgcggta
     7021 gaagttgaca tcgatggcgg gaataccgta tccggacact tccttatccg gactcatttt
     7081 gacgacgggg atgccgtctg tctgttccaa gccgaggcgc ggttcgccgt caacgtggcg
     7141 caacaccaat acctggtccg gataaatcag gtcgggattg tggatttgat cccggttcgc
     7201 gtcccacagg cggctccatt gccacgggct gtacaggtat ttgcccgaaa tgccccacag
     7261 ggtgtcgccc tgtttgaccg tgtagcgttc cggcgcgttc gggcgcacct ccaaatttgc
     7321 cgccaaagtt tgtgttgaga atgccatacc tgccgcgcag agcagggtta taatacgacg
     7381 ttgcataacc gttcccctta tctgataaat ttcggtttgt cttgcttgat tgggttggaa
     7441 aaagcggcgg cagcccctcg ggatgtgccg cgtgataaaa aatgttccgc attttaacat
     7501 cgaattatcc gcaccatcac ggtaattatg aaaaacaggc ggcgtatccg ccgaaggaaa
     7561 gagaaaatta tggctttatt gaatatcttg caatatcccg acgagcgtct gcacacggtg
     7621 gcaaagcctg tcgaacaagt cgacgagcgc atccggaagc tgattgccga tatgtttgaa
     7681 acgatgtacg aatcgcgcgg catcgggctg gcggcgacgc aggtcgatgt gcacgagcgc
     7741 gtggtcgtga tggatttgac cgaagaccgc agcgaaccgc gcgtgttcat caaccccgtc
     7801 atcgttgaaa aagacggcga aaccacttac gaagagggct gcctgtccgt gccgggcatt
     7861 tacgacaccg taacccgcgc cgaacgcgtc aaggtcgagg ctttgaacga aaaaggcgaa
     7921 aagttcacgc tggaggcgga cggcttgctg gcgatttgcg tgcagcacga gttggaccac
     7981 ctgatgggca tcgtgtttgt cgaacgcctg tcgcagctca agcagggacg catcaaaacc
     8041 aagctgaaaa aacgtcagaa acatacgatt tgaccctttt gccgtgccgt ctgaacgctg
     8101 caaagttttc agacggcacg gtcttgtccg acaaccttac gcatacgcag gaatatgcta
     8161 tgaaagtcat ctttgccggc acgcccgatt ttgccgccgc cgccttaaaa gccgttgccg
     8221 ccgccggttt tgaaatcccg ctggtgctga cccagcccga ccgtccgaaa gggcgcggta
     8281 tgcaactgac cgccccgccc gtcaaacaag ccgcgctgga actcggtttg cgcgtggcac
     8341 agcccgaaaa actgcgtaac aacgccgaag ccctgcaaat gctcaaagag gtcgaggcag
     8401 acgtaatggt ggttgccgcc tacggtttga ttctgccgca ggaagtgttg gatacgccga
     8461 aacacggctg cctcaacatc cacgcttcgc tgttaccccg ttggcgtggc gcggcgccga
     8521 ttcaacgcgc gattgaagcc ggcgatgccg agacaggcgt gtgtattatg cagatggaca
     8581 tcggtttgga caccggcgat gtggtcagcg aacaccgcta cgccatccaa ccgaccgata
     8641 ccgccaacga agtccacgac gcgctgatgg aaatcggtgc ggcggcggtt gttgccgatt
     8701 tgcaacagct tcaaagcaaa ggccgtctga acgcggtcaa acagcccgaa gaaggtgtta
     8761 cttacgccca aaaactaagc aaagaagagg cgcgtatcga ttggagcgaa agcgcggcgg
     8821 ttatcgaacg caaaatccgc gccttcaacc ccgtgcctgc cgcgtgggtt gagtatcagg
     8881 gcaagccgat gaaaatccgg cgggcggaag tggtggcgca acaaggcgcg gcaggcgaag
     8941 tgttgtcctg ttcggcggac ggtttggtcg ttgcctgcgg cgaaaacgcg ctgaagatta
     9001 ccgaattgca gcctgccggc ggcaggcgta tgaatatcgc ggcgtttgcg gcgggacggc
     9061 atatcgaagc agggacgaag ctgtaaatcc cttcagacgg cattccgatc cgcaaacggg
     9121 aatgccgtct gaaaccatca gttgaagaaa gcgaatcaca taatatgagt atggcacttg
     9181 cccaaaaact tgccgccgac agcattgcgg cggttgccga aggacgcaac cttcaggacg
     9241 tgttggcgca aatccgcacc gcgcatcccg accttacggc gcaggaaaac ggcgcgttgc
     9301 aggacatcgc ctacggttgc cagcgttatt tgggcagttt gaaacatatg ctcgcgcaga
     9361 tgctgaaaaa accgattgac aatccgcagc tcgaaagcct gcttttggcg gcgttgtacc
     9421 aactgcatta cacgcgcaac gcgccgcacg ccgtggtcaa tgaggcggtg gaaagcatag
     9481 ccaaaatcgg gcgcgggcag taccgttcgt ttgccaacgc ggttttgcgc cgctttttgc
     9541 gcgaacgcga caagctcgcg gcttcctgta aaaaagacga tgtggcgaaa cacaacctgc
     9601 cgctgtggtg ggtggcttac ctgaaaaacc attatccgaa acactggcac aacatcgccg
     9661 ccgcgctgca atcccatccg ccgatgacgt tgcgcgtcaa ccgccggcac ggcaacgccg
     9721 aaagctattt ggaaaaactg gctgcggaag gcattgcggc taaggcgttg gacgaatatg
     9781 cggttacgtt ggaagaagcc gtgccggtaa accgcctgcc cggtttttca gacggcatcg
     9841 tttcggtaca ggacttcggc gcgcagcagg cggcgtatct ccttaacccg aaagacggcg
     9901 aacggatttt ggacgcgtgc gccgcgccgg gcggcaagac ggggcatatc ttggaactgg
     9961 cggattgccg cgttaccgcc ttagacattg atgcaggccg tctgaagcgt gtggaggaca
    10021 atatcgcccg tttgggcttt cagacggcat cggcggcgtg tgccgatgcg cgggatttgg
    10081 cggcgtggta tgatggaaag cctttcgacg caatccttgc cgacgtgcca tgcaccgctt
    10141 ccggcgtggc gcggcgcaat cccgacgtga aatggctgcg ccgtccgacc gacgcgctca
    10201 aaaccgcccg ccagcaggaa gccctgctgg acgcactttg gcagacgctg aaacagggcg
    10261 gcagaatgct gcttgccacc tgttccgtgt tcgtcgaaga aaacgacggg caattgcaaa
    10321 aattcctcaa ccgccatgcc gatgccgaac cgatcgaatc gcgggtgctt ttaccgaaca
    10381 aacaccaaga tggcttttat tacgcgctta ttcaaaagca ttaaacaatg gcttgtgctg
    10441 ctgccgatgc tttccgtttt gccggacgcg gcggcggagg ggatagatgt gagccgcgcc
    10501 gaagcgagga taaccgacgg cgggcagctt tccatcagca gccgcttcca aaccgagctg
    10561 cccgaccagc tccaacaggc gttgcgccgg ggcgtgccgc tcaactttac cttaagctgg
    10621 cagctttccg ccccgataat cgcttcttat cggtttaaat tggggcaact gattggcgat
    10681 gacgacaata ttgactacaa actgagtttc catccgctga ccaaccgcta ccgcgttacc
    10741 gtcggcgcgt tttcgacaga ctacgacacc ttggatgcgg cattgcgcgc gaccggcgcg
    10801 gttgccaact ggaaagtcct gaacaaaggc gcgctgtctg gtgcggaagc aggggaaacc
    10861 aaggcggaaa tccgcctgac gctgtccact tcaaaactgc ccaagccttt tcaaatcaat
    10921 gcattgactt ctcaaaactg gcatttggat tcgggttgga aacctctaaa catcatcggg
    10981 aacaaataat gcgccgtttt ctaccgatcg cagccatatg cgccgtcgtc ctgttgtacg
    11041 gactgacggc ggcaaccggc agcaccagtt cgctggcgga ttatttctgg tggatagttg
    11101 cgttcagcgc aatgctgctg ctggtgttgt ccgccgtttt ggcacgttat gtcatattgc
    11161 tgttgaaaga caggcgcgac ggcgtattcg gttcgcagat tgccaaacgc ctttctggga
    11221 tgtttacgct ggttgccgta ctgcccggcg tgtttctgtt cggcgtttcc gcacagttta
    11281 tcaacggcac gattaattcg tggttcggca acgataccca cgaggcgctt gaacgcagcc
    11341 tcaatttgag caagtccgca ttgaatctgg cggcagacaa cgcccttggc aacgccatcc
    11401 ccgtgcagat agacctcatc ggcgcggctt ccctgcccgg ggatatgggc agggtgctgg
    11461 aacattacgc cggcagcggt tttgcccagc ttgccctgta caatgccgca agcggcaaaa
    11521 tcgaaaaaag catcaacccg cacaagctcg atcagccgtt tccaggtaag gcgcgttggg
    11581 aaaaaatcca acgggcgggt tcggtcaggg atttggaaag cataggcggc gtattgtacg
    11641 cgcagggctg gctgtcggcg ggtacgcaca acgggcgcga ttacgccttg tttttccgtc
    11701 agccggttcc caaaggcgtg gcagaggatg ccgtcttaat cgaaaaggca agggcgaaat
    11761 atgctgagtt gagttacagc aaaaaaggtt tgcagacctt tttcctggca accctgctga
    11821 ttgcctcgct gctgtcgatt tttcttgcac tggtcatggc actgtatttc gcccgccgtt
    11881 tcgtcgaacc cgtcctatcg cttgccgagg gggcgaaggc ggtggcgcaa ggcgatttca
    11941 gccagacgcg ccccgtgttg cgcaacgacg agttcggacg cttgaccaag ttgttcaacc
    12001 acatgaccga gcagctttcc atcgccaaag aagcagacga gcgcaaccgc cggcgcgagg
    12061 aagccgccag acattatctt gaatgcgtgt tggaggggct gaccacgggc gtggtggtgt
    12121 ttgacgaaca aggctgtctg aaaaccttca acaaagcggc ggaacagatt ttggggttgc
    12181 cgcttacccc cctgtggggc agcagccggc acggttggca cggcgtttcg gcgcagcagt
    12241 ccctgcttgc cgaagtgttt gccgccatcg gcgcggcggc aggtacggac aaaccggtcc
    12301 atgtgaaata tgccgcgccg gacgatgcca aaatcctgct gggcaaggca accgtcctgc
    12361 ccgaagacaa cggcaacggc gtggtaatgg tgattgacga catcaccgtt ttgatacacg
    12421 cgcaaaaaga agccgcgtgg ggcgaagtgg ccaaacggct ggcacacgaa atccgcaatc
    12481 cgctcacgcc catccagctt tccgccgaac ggctggcgtg gaaattgggc gggaagctgg
    12541 acgagcagga cgcgcaaatc ctgacgcgtt cgatcgacac catcatcaaa caggtggcgg
    12601 cgttaaaaga aatggtcgaa gcattccgca attacgcgcg cgcgccgtcg ctcaaattgg
    12661 aaaatcagga tttgaacgcc ttaatcggcg atgttttggc tctgtacgaa gccggcccgt
    12721 gccggtttga ggcggaactt gccgacgaac cgctgatgat ggcggcggat acgaccgcca
    12781 tgcggcaggt gctgcacaat attttcaaaa atgccgccga agcggcggaa gaagccgata
    12841 tgcccgaagt cagggtaaaa tcggaagcgg ggcaggacgg acggattgtc ctgacggttt
    12901 gcgacaacgg caaagggttc ggcagggaaa tgctgcacaa cgccttcgag ccgtatgtaa
    12961 cggacaaacc ggcgggaacg ggattgggac tgcccgtggt gaaaaaaatc attgaagaac
    13021 acggcggacg catcagcctg agcaatcagg atgcgggtgg ggcgtgtgtc agaatcatct
    13081 tgccaaaaac ggtagaaact tatgcgtagc agcgatattt taattgtaga cgacgaagtc
    13141 ggcatccgcg acctgctgtc ggaaatcctg caggacgaag gctattcggt cgcattggcg
    13201 gaaaatgccg aagaggcgcg caagctgcgc catcaggcgc gtcccgcgat ggtgctgctg
    13261 gatatttgga tgcccgattg cgacggcatc acgcttttga aggagtgggc gaaaaacggg
    13321 cagctcaata tgccggtggt gatgatgagc gggcatgcca gcatcgatac cgccgtagaa
    13381 gcgaccaaaa tcggcgcgat cgattttttg gaaaaaccga tttccctgca aaagctgctg
    13441 tctgccgtcg aaaacgcgtt gaaacatggt gcggcgcaaa tcgaaacggg accggtgttc
    13501 gacaagctgg gcaacagtgc cgcgattcag gagatgaacc gcgaagtcga agcggcggca
    13561 aagcgcacgt cccctgtatt tttgacgggc gaagcaggtt cgccgtttga aaccgtggcg
    13621 cgctatttcc ataaaaacgg tacgccgtgg gtcagtccgg caagggtcga atatctgatt
    13681 gatatgccga cggaactgtt gcagaaagct gaaggcggtg ttttatatgt gggcgatatt
    13741 gcccaataca gccgcaacat ccaagccggc attgccttta ttgtcggcaa ggcggaacac
    13801 cgccgcgtca gggtggtcgc atcgggcagc agggcggcag gttcagacgg tattgcctgc
    13861 gaggaaaagc tggcggaact gctgtcggag tcggtcgtcc gcattccgcc gctgcgtatg
    13921 cagcatgaag atattccctt cctgatacag gggattgcct gcaatgtggc ggaaagtcaa
    13981 aagattgcgc ccgccgcatt cagtgaagag gcacttgccg cattgacccg ttacgactgg
    14041 ccgggaaatt tcgaccaact gcaaagcgtc gttgcaacgc tgttgttgga ggcggacgga
    14101 caggaaatcg gcgcggcggc ggtttcttcc cttttggggc agaatgtgcc tgccgaggga
    14161 gcggaagata tggtgggcgg gtttaatttc aacctgcccc tgcgcgaatt gagggaggag
    14221 gtggagcggc gttatttcga gtaccacatc gcccaagaag gtcagaatat gagcaaagtg
    14281 gcgcagaaag tcggtttgga acgcacgcac ctttaccgca aactcaaaca gctcggcatc
    14341 ggcgtttcgc gccgggcggg ggaaaaaacc gaagaatagg cccggacggc cggttttccg
    14401 gctgcgggct tttgttttca gacggcattt ggtgcaaatg ccgtctgaaa tcgtaagggg
    14461 acggatttta tgacagagga cgaacgtttc gcgtggctgc aattggcgtt tacgccctat
    14521 atcggcgcgg aaagcttcct gctgctgctg cgccgtttcg gcagcgcgtc aaacgccctg
    14581 tccgcaccgg cggaacaggt cgccccggcg gtgcggcaca agcaggcggc cgaggcttgg
    14641 cgcaatgcgg aaaaacgcgc tttggcgcgg caggcggcag aagcggcgtt ggaatgggaa
    14701 atgcgggacg gatgccgcct gatgctgctt caggatgaag atttccccga aatgctgacg
    14761 caggggctga ccgcgccgcc ggttttgttt ttgcgcggca acgtgcggct gctgcacaag
    14821 ccttccgccg ccatcgtcgg cagccgccat gccacgccgc aggcgatgcg gattgccaaa
    14881 gatttcggca gggcgttggg agagaaaggc attcccgttg tgtcgggtat ggcttcgggc
    14941 atcgataccg ccgcccatca gggtgcgttg caggcggaag gcggcaccat cgccgtgtgg
    15001 gggacgggca tagaccgcat ttatccgccg tccaataaaa accttgccta tgaaatcgcc
    15061 gaaaaaggac tgattgtcag cgagttcccc atcggcacgc gcccgtatgc cggcaatttt
    15121 ccgcgccgca accgcctgat tgccgcgctg tcgcaggtta cgctggtggt tgaagccgcc
    15181 ttggaatccg gttcgctgat taccgccggc ttggcggcag aaatggggcg cgaagtgatg
    15241 gcggtacccg gctcgataga caatccgcac agcaaaggct gccacaaact gattaaagac
    15301 ggcgcgaaac tgacggaatg tttggacgac atcctgaacg aatgcccggg gctattgcaa
    15361 aatacgggtg cttcatcata ttctataaat aaggatacgc ccgacacggg ccgccgcgcc
    15421 gttcagacgg catacgcccc gccgcctgcc gcaaaaatgc cgtctgaagg ggcggcaggc
    15481 ggcacagccg tcggcggcat actggataaa atgggtttcg accccatcca tcccgacgtg
    15541 cttgccggac agttggctat gcctgccgca gatttgtatg ccgcactgtt ggaattggaa
    15601 ttggacggca gcgttgccgc aatgcccggc ggcagatacc agcgtatccg aacttgaacg
    15661 cactttatat taaggaacac gaatgaccga agtcatcgcc tacctcatcg aacatttcca
    15721 agatttcgat acctgtccgc cgcccgaaga cttgggtatg ctgcttgaag aagcgggttt
    15781 cgatacgatg gaaatcggca acaccctgat gatgatggaa gtattgctca acagctccga
    15841 attttccgcc gaacctgccg acagcggcgc attgcgcgtg tacagcaaag aagaaaccga
    15901 caacctgccg caggaagtga tggggctgat gcagtatctg attgaagaaa aagccgtcag
    15961 ctgcgaacag cgggaaatca tcatccacgc gctgatgcac attccgggcg acgaaattac
    16021 cgtagatacc gccaaagtgc tgaccctgct gcttttatgg gcaaacaaga gcgagctgcc
    16081 cgtgttggtc ggcgacgagc tgatgagcgc gcttttactc gacaacaaac ccacgatgaa
    16141 ctgaagcggc ttcagacggc attatcctgc taacctgccg tctgaaacgc cggcatcaaa
    16201 accaccatcc agagaacgac aaatggcgaa aaacctatta atcgtcgaat ccccgtccaa
    16261 agccaaaacc ctgaaaaaat atttgggcgg cgattttgaa atccttgcat cctacggaca
    16321 cgtccgcgac ctcgtcccca aaagcggcgc ggtcgatccc gacaacggct ttgcgatgaa
    16381 ataccaactc atcagccgca acggcaaaca cgtcgatgcc atcgtcgccg gtgccaaaga
    16441 agctgaaaac atctacctcg ccaccgaccc ggatagggaa ggcgaagcca tttcctggca
    16501 tcttttggaa atcctcaaat ccaaacgcgg cttgaaaaac atcaagccgc agcgtgtcgt
    16561 gttccacgaa atcaccaaaa acgccgtgct cgatgccgtt gcccatccgc gcgaaatcga
    16621 aatggacttg gtggatgcac aacaagcccg tcgcgctttg gactatttgg tcggctttaa
    16681 cctttcgcca ttgttatgga aaaaaatccg ccgcggtttg agcgcaggcc gcgtgcaaag
    16741 ccccgctttg cgcctgattt gtgagcgcga aaacgaaatc cgcgcgtttg aagcgcagga
    16801 atattggacg gtgcatctcg acagccacaa aggccgcagc aagttcaccg ccaaactcgc
    16861 ccaatacaac ggcgcgaaac tcgaacaatt cgacctgccg aacgaagccg cgcaagccga
    16921 cgtgttgaaa gaactcgaag gcaaagaggc cgtcgttacc gccattgaaa agaaaaagcg
    16981 cagccgcaat cctgccgcgc cgttcaccac ttctaccatg cagcaggatg ccgtgcgcaa
    17041 actcggcttc accaccgacc gcaccatgcg taccgcccaa cagctttatg aaggtataga
    17101 cgtagggcag ggcgcgatcg gtctgattac ctatatgcgt actgacagcg tgaatttggc
    17161 ggatgaagcg ttaaccgaaa tccgccatta catcgaaaac aaaatcggca aagaatatct
    17221 gccgagtgcc gccaaacaat acaaaaccaa atccaaaaac gcccaagaag cgcacgaagc
    17281 catccgtccg acttccgtgt accgtacgcc cgaaagcgtc aaacccttcc tgagcgcaga
    17341 ccagttcaaa ctctatcaaa tgatttggca gcgtaccgtc gcctgtcaga tgacgcctgc
    17401 caaattcgac caaaccaccg tcgatattac cgtcggcaaa ggcgtattcc gcgtaaccgg
    17461 acaagtgcaa accttcgcag gcttcttgag cgtttatgaa gaaagcagcg acgatgaaga
    17521 aggcgaagac agcaaaaaac tgcccgaaat gagcgaaggc gataaattgc ccgtggacaa
    17581 actctacggc gaacaacact ttaccactcc gccgccgcgc tacaacgaag ccacgctggt
    17641 taaagccctc gaagaatacg gcatcggccg cccctcgacc tacgccagca tcatctccac
    17701 gctcaaagac cgcgaatacg ttacccttga gcaaaaacgc ttcatgccca ccgacacagg
    17761 cgacatcgtc aacaaattcc tgaccgaaca cttcgcccaa tacgtcgatt accacttcac
    17821 cgccaaactc gaagaccagc ttgacgaaat tgccgacggc aaacgccaat ggattccctt
    17881 gatggacaaa ttctggaaac cgttcatcaa acaagtggaa gaaaaagaag gcatcgaacg
    17941 cgccaaattt accacgcagg aacttgatga aacctgcccg aaatgcggcg aacacaaact
    18001 gcaaatcaaa ttcggcaaaa tgggtcgttt tgttgcgtgt gccggttatc ccgagtgcag
    18061 ctacacgcgc aatgtcaacg aaaccgccga agaggccgcc gagcgcatcg ccaaagccga
    18121 agccgaacag gccgaactcg acggacgcga gtgcccgaaa tgtggcggtc gcctagtgta
    18181 caaatacagc cgcaccggca gcaaattcat cggctgcgcc aactatccga aatgcaaaca
    18241 cgtcgagccg ctggaaaaac cgaaagatac cggcgtccag tgtccgcaat gcaaaaaagg
    18301 caacctcgtc gagcgcaaat cccgctacgg caaactgttt tacagttgca gcacctatcc
    18361 cgactgcaac tacgccactt ggaacccgcc cgttgccgaa gaatgcccga actgccattg
    18421 gccggtcttg accatcaaaa ccaccaaacg ctggggcgtc gaaaaagtct gcccgcaaaa
    18481 agaatgcggc tggaaagaac agattgaacc gcccgcgccg aaggagtaag attaggttgg
    18541 tttgaaagag aaaaggtcgt ctgaaaaatt ttcagacgac ctttgctttt ctgtgattgg
    18601 tttatttgaa tccgcgtgtt gttttaaagt ccgataaaat ccggttcatt tcaggcacaa
    18661 acaaggcgat gtaatcgtaa gatagaccgc gactggcact gggatgggga aagcagacga
    18721 cttcgcaatc ttcaaacgat tggaatttga cattgaaacg tgtaccgtca aattcttttt
    18781 gcaccgtctc cagcggtttg gtctgcttac cgaccaactg ctcgaagcgt ggcagtacat
    18841 tttggttgtt cagaaaatcc gccaacctgc tgcccatgaa gaggatgact ttcggacgca
    18901 gtttttcgat gtggtagaga aaattatcga tgtgctcggg ttgtgtgaac ttgtcgggat
    18961 tgtcgatagt gttgccctgt gtagcagccc agttggtttg aaccagggat ttttcaaatg
    19021 caccgcccaa tccattttcg tctaaggggt gtccccacat ttcaaaccaa ttttttatcg
    19081 tattgtcgta acgccacttt tttgcctgct ctccgaaata gagggatttg tttgcaaatg
    19141 tatggtcgat tttgttttca gggagtttgt attcacctgc tacataagca gcctcatcgg
    19201 ctttactcca accccattca tagccacaaa tcattaagcc atgtttgtcg ttgtagcctt
    19261 tgaacaggct gttgctcaaa ttcaaatcct tcatcatgaa ctcttccttt taaaatttaa
    19321 gagcgattga cttcaatgtt tttagatggg gtggaaaaat ccttgtgtag gcaacataaa
    19381 ttcaataaat ttcttgataa ttcgaaacct actaatagcg cacctataaa agctttttca
    19441 ttacgttcag catgacggtc acgtcgttca tattttttac gcttgctgtt ccctgttatt
    19501 acagctaagc caagtgatat ggcgagaatt gcccaaacaa tagtacttaa taacaatttt
    19561 ccccatacta tcaataggga aagaaaaaac cttttagtat taggtcgata gattataatc
    19621 catgcccatg aaaatgcaat aagagttata cataagatac aagctgatag gatttcttta
    19681 tttttaatta aataacttag ggcgatgagg atgacaggtg tcaagaaaac aatagttaca
    19741 tcccgatagc tataaaagaa aactgcccca ttttgaatgt ggaaatgtgc acagaatcca
    19801 atatagctaa ggataatagt taatataata aaaaaagacc accaagggtg aagagatagg
    19861 aattccatgt tttccctttt tttgtaaaaa ggaaaaaatc ttatctaata gttaattgcc
    19921 taaccagcca gaagaagttt aaaatctatc ccaataattc aaccatctat acagaaagtt
    19981 cagcttatgg aaacccacga aaaaatccgc ctgatgcgcg aattgaataa atggtcccag
    20041 gaggatatgg cggaaaagct ggcgatgtcg gcaggcgggt atgccaaaat cgaacgaggc
    20101 gaaacgcagt tgaatatccc gcgtttggag cagttggctc agattttcaa aatcgatatg
    20161 tgggacttgc tcaaatcggg cggtggtggg atggtgtttc agattaatga aggtgatagt
    20221 ggtggcgata ttgcgttgta tgcgtcgggt gatgtttcga tgaaaataga atttttaaaa
    20281 atggagttga aacactgcaa agaaatgttg gaacaaaaag acaaagaaat cgagctgctc
    20341 cgcaagctga ccgaaaccgt ttaaacagat atgccgtctg aaaaaagttt tcagacggca
    20401 tattctttga caggtcttgt ataataccgt ttgaacttac aggtttttga ttatggcggc
    20461 aggcaaacat accaaacaca gcaaccgggt acgcattatc ggcgggcaat gccggggcag
    20521 gaaattgagt ttcacatccg ccgacggact gcgtccgaca cccgacagcg tgcgtgaaaa
    20581 gctgtttaac tggctgggac aggatttgac gggtaaaacg gttttggatc tcttcggagg
    20641 cagcggcgca ctcggtatag aagccgcttc gcgcaacgcc aaacgcgtgc tgatttcgga
    20701 taacaaccgc caaaccgtgc agaccttgca gaaaaacagt cgcgaactgg gtttggggca
    20761 ggtgcaaatc gtcttttcag acggcatcgc atatttgaag accgtatccg aacagtttga
    20821 tgttgtcttt ctcgacccgc cgtttgcatg gcaggactgg caaatcctgt tcgatgcctt
    20881 gaagccgtgc ctgaaccccc gggcattcgt ctatctcgag gcgggtacgc tgccgaatat
    20941 tcccgattgg ctgacggaat atagagaagg gaaatcgggg cagagtacat tcgaattaag
    21001 ggttttccaa gtggctgaat aatatgcgct ttgataatca tttccgagtt gtaaacattc
    21061 gtttgcaacc gtccggttca aaaaaacctt gtgctataat ccgcgcccgc ccggttttga
    21121 taatttagtg gaaaaggaaa agaaatgtcg ctttttatta ccgacgagtg catcaactgc
    21181 gacgtatgcg aacccgaatg ccccaatgat gccatttccc aaggcgagga aatttacgaa
    21241 atcaacccca acctctgcac gcagtgcgtc ggacactacg atgagccgca gtgccagcag
    21301 gtttgcccgg tggactgcat cctgattgac gaagaacatc ccgaaaccca tgacgagttg
    21361 atggcgaaat acgaaaagat tatccagttt aaataaattc tttttaaaac atcaaattat
    21421 gtctgttttg aaataaaatc aaaaaaaaac ttgacggaaa agcaagccgc taataaacta
    21481 acgttctctt ttggagggat tcccgagcgg tcaaaggggg cagactgtaa atctgttgcg
    21541 aaagcttcga aggttcgaat ccttctccct ccaccaaaat tcttacttgg ggcagtagcg
    21601 agtaatgcgg gtgtagctca atggtagagc agaagccttc caagcttacg gtgagggttc
    21661 gattcccttc acccgctcca aacaattagg cccatgtagc tcaggggtag agcactccct
    21721 tggtaaggga gaggtcggca gttcaaatct gcccatgggc accatccctc gattattcat
    21781 ttctttaagg cttagatata taggatattg ccatggctaa ggaaaaattc gaacgtagca
    21841 aaccgcacgt aaacgttggc accatcggtc acgttgacca tggtaaaacc accctgactg
    21901 ccgctttgac tactattttg gctaaaaaat tcggtggtgc tgcaaaagct tacgaccaaa
    21961 tcgacaacgc acccgaagaa aaagcacgcg gtattaccat taacacctcg cacgtggaat
    22021 acgaaaccga aacccgccac tacgcacacg tagactgccc ggggcacgcc gactacgtta
    22081 aaaacatgat taccggcgcc gcacaaatgg acggtgcaat cctggtatgt tccgcagccg
    22141 acggccctat gccgcaaacc cgcgaacaca tcctgctggc ccgtcaagta ggcgtacctt
    22201 acatcatcgt gttcatgaac aaatgcgaca tggtcgacga tgccgagctg ttggaactgg
    22261 ttgaaatgga aatccgcgac ctgctgtcca gctacgactt ccccggcgac gactgcccga
    22321 ttgtacaagg ttctgcactg aaagccttgg aaggcgatgc cgcttatgaa gagaaaatct
    22381 tcgaactggc tgccgcattg gacagctaca tcccgactcc cgagcgtgcc gtggacaaac
    22441 cgttcctgct gcctatcgaa gacgtgttct ccatttccgg ccgaggtaca gtagtaaccg
    22501 gccgtgtaga gcgcggtatc atccacgttg gtgacgagat cgaaatcgtc ggtctgaaag
    22561 aaacccaaaa aaccacctgt accggtgttg aaatgttccg caaactgctg gacgaaggtc
    22621 aggcgggcga caacgtaggc gtattgctgc gcggtaccaa acgtgaagac gtggaacgcg
    22681 gtcaggtatt ggccaaaccg ggtactatca ctcctcacac caaattcaaa gcagaagtgt
    22741 acgtactgag caaagaagag ggcggccgcc atactccgtt cttcgccaac taccgtccgc
    22801 aattctactt ccgtaccacc gacgtaaccg gcgcggttac tttggaagaa ggtgtggaaa
    22861 tggtaatgcc gggtgagaac gtaaccatta ccgtagaact gattgcgcct atcgctatgg
    22921 aagaaggcct gcgctttgcg attcgcgaag gcggtcgtac cgtgggtgcc ggcgtg
//
LOCUS       DAXIHS010000025        22795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXIHS010000025 DAXIHS010000000
VERSION     DAXIHS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.025"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..108
                     /locus_tag="ACFOF2_000869"
     CDS             <1..108
                     /locus_tag="ACFOF2_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC7107216.1"
                     /translation="VPKKAETISNKLFFTHHPKRQTEFAVRQIRQARAE"
     gene            234..1529
                     /gene="hisS"
                     /locus_tag="ACFOF2_000870"
     CDS             234..1529
                     /gene="hisS"
                     /locus_tag="ACFOF2_000870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC7107217.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            1530..2159
                     /locus_tag="ACFOF2_000871"
     CDS             1530..2159
                     /locus_tag="ACFOF2_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC7107218.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            2313..3770
                     /gene="der"
                     /locus_tag="ACFOF2_000872"
     CDS             2313..3770
                     /gene="der"
                     /locus_tag="ACFOF2_000872"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC7107219.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3801..4700
                     /locus_tag="ACFOF2_000873"
     CDS             3801..4700
                     /locus_tag="ACFOF2_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC7107220.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLEWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4752..5168
                     /locus_tag="ACFOF2_000874"
     CDS             4752..5168
                     /locus_tag="ACFOF2_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC7107221.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            5234..5800
                     /gene="dcd"
                     /locus_tag="ACFOF2_000875"
     CDS             5234..5800
                     /gene="dcd"
                     /locus_tag="ACFOF2_000875"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC7107222.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5864..6568)
                     /locus_tag="ACFOF2_000876"
     CDS             complement(5864..6568)
                     /locus_tag="ACFOF2_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC7107223.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            6714..7959
                     /locus_tag="ACFOF2_000877"
                     /pseudo
     CDS             6714..7959
                     /locus_tag="ACFOF2_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            8098..9051
                     /locus_tag="ACFOF2_000878"
     CDS             8098..9051
                     /locus_tag="ACFOF2_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC7107224.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9162..9485)
                     /locus_tag="ACFOF2_000879"
     CDS             complement(9162..9485)
                     /locus_tag="ACFOF2_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107225.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(9658..11019)
                     /locus_tag="ACFOF2_000880"
     CDS             complement(9658..11019)
                     /locus_tag="ACFOF2_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC7107226.1"
                     /translation="MLKKWLNKMLPSSRSSKEAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKEAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(11374..13074)
                     /gene="recJ"
                     /locus_tag="ACFOF2_000881"
     CDS             complement(11374..13074)
                     /gene="recJ"
                     /locus_tag="ACFOF2_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC7107227.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAAGRLADAVERKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(13143..13859)
                     /locus_tag="ACFOF2_000882"
     CDS             complement(13143..13859)
                     /locus_tag="ACFOF2_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107228.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVDIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(13906..14412)
                     /gene="yjgA"
                     /locus_tag="ACFOF2_000883"
     CDS             complement(13906..14412)
                     /gene="yjgA"
                     /locus_tag="ACFOF2_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC7107229.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            14566..15897
                     /gene="pmbA"
                     /locus_tag="ACFOF2_000884"
     CDS             14566..15897
                     /gene="pmbA"
                     /locus_tag="ACFOF2_000884"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC7107230.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            15909..16100
                     /locus_tag="ACFOF2_000885"
     CDS             15909..16100
                     /locus_tag="ACFOF2_000885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107231.1"
                     /translation="MPSEDEGSTFCPVPARLPFYAAPPFADNIAHFFISTDFPLSVKI
                     LSHLSTTGYTQVQDKACGD"
     gene            complement(16194..16397)
                     /locus_tag="ACFOF2_000886"
     CDS             complement(16194..16397)
                     /locus_tag="ACFOF2_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC7107232.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            16640..16942
                     /gene="clpS"
                     /locus_tag="ACFOF2_000887"
     CDS             16640..16942
                     /gene="clpS"
                     /locus_tag="ACFOF2_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC7107233.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            16944..19235
                     /gene="clpA"
                     /locus_tag="ACFOF2_000888"
     CDS             16944..19235
                     /gene="clpA"
                     /locus_tag="ACFOF2_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC7107234.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(19275..22373)
                     /locus_tag="ACFOF2_000889"
     CDS             complement(19275..22373)
                     /locus_tag="ACFOF2_000889"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC7107235.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDSQTG
                     EARRGYLDVAKELRERFPDPDEIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLSRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEDLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(22452..>22795)
                     /locus_tag="ACFOF2_000890"
     CDS             complement(22452..>22795)
                     /locus_tag="ACFOF2_000890"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC7107236.1"
                     /translation="NYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLINTS
                     SLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDILTHSISEGLPHEIALRRKQYEYY
                     REQLLAFPKAT"
BASE COUNT         5566 a         6374 c         5607 g         5248 t
ORIGIN      
        1 gtgccgaaaa aagccgagac aatttcaaat aaattgtttt tcacacatca tcccaagcgg
       61 cagacagagt ttgcagtccg acaaatcagg caagcacgtg cagagtaagt caaatgccgt
      121 ctgaaaactg tttcagacgg cattttaccc gtccgcaaac cacccccaac ctcacaaacc
      181 cttaaaaatc cgctataatc gcccgcaatt tgatttaagc attcttactt gaaatggcac
      241 aaaaaatcca atccgtcaaa ggcatgaacg accttctgcc tgtcgagcaa aaagatttca
      301 aactgacggc tgcgttttgg caggcgtttg aggatacggt aaaccgctgg acgcgcgctt
      361 acggctatca gcaaatccgt acgccgattg tcgagcaaac cggtttgttt gtccgctcca
      421 tcggcgagga aaccgatgtg gtcggcaagg aaatgtacac cttttccgat tcaaacgatt
      481 ctttgagttt gagcctgcgt ccggaaggta cggcttcttg tctgcgtgcg gtggtcgaac
      541 acaaccttct gtacaacagc ccgcaaaagc tgtggtatat ggggccgatg ttccgccgcg
      601 agcgtccgca aaaaggccgt tatcgtcaat tccatcaggt cggtatcgag gctttgggtt
      661 ttgaagggcc ggatatcgat gcggaaatca tcgcgatgtc tgccgacttg tgggaaaaat
      721 tgggtatccg cgaatacctg actttggaaa tcaacagctt aggcaaccgt gaggagcgag
      781 cggcacaccg tgcggcgttg gttgaatatt tgacacgcta tgaagataaa ttggacgaag
      841 acagcaaacg ccgtctgaaa accaatcctt tgcgtgtttt agatacgaaa aaccctgatt
      901 tgcaggaaat ctgcaacgcc gcgccgcgtt tggtggatta tttgggcgag gcttcgcaaa
      961 accactatgc acgcttcaag gcgatgttgg acggtttggg tatccaatat attgaaaatc
     1021 cgcgcttggt tcgcggtttg gattattaca atcagacggt ttttgagtgg acgaccgaca
     1081 aactcggcgc gcaggcaact gtgtgcggcg gcggccgtta cgatggtttg attgaggaac
     1141 ttggcggcaa acctgcgccg tccatcggct ttgcgatggg catcgaacgg ctgctgcttt
     1201 tggtgagcga atacggctcg ctggaagtga acgctgcgcc tgatgtctat gcaatgcacc
     1261 aaggcgaaag ggcggacttg caggtgatga aatacgcgca agccttgcgc gcgcaaggtt
     1321 tcaatgtaat gcagcattcc ggctatcaaa gcctgaaagc gcaaatgaaa aaagccgaca
     1381 acagcggcgc gcgctttgcc ctgattgtcg cgcaagacga actggcgaac ggtacggtta
     1441 cgctcaaaga catgaacggc gcacacggtc agcaaactgt cgccgccgag gatttaaccc
     1501 atactttaca acaatggaag aacgcataaa tggcagccca tctcgaagaa caacaagagt
     1561 tagacaactt taaatatttt tggaaaacca cgggcaaatg gctgtttgcc ctgctgattt
     1621 tggcagcact cggctacttg ggatacacgg tttaccaaaa ccgtgcggct tcccaaaatc
     1681 aggaagcggc ggcggtgctg gcaaacatcg tggaaaaggc gcaaaacaaa gccccgcaaa
     1741 gcgaaattaa tgccgaattg accaaactcc aacaaagcta cccgcattct atttccgccg
     1801 cccaagccac gctgatggcg gcggcaaccg aatttgacgc gcagcgttac gatgttgccg
     1861 aaggccattt gaaatgggtg ttgtccaacc aaaaagacag cctgattcaa gcgttggcgg
     1921 cgcagcgtct gggcgttgtg ttgttgcaac aaaaaaaata cgatgccgcg cttgccgcgc
     1981 tcgacacgcc ggttgaagcg gacttctccc ccctgctgat ggaaaccaaa ggcgatgtct
     2041 atgccgcaca gggaaaaagc caggaagcct taaaaaacta cggtcaggct ttagaaaaaa
     2101 tgcctcaaga ttctgtcggt cgtgaattgg ttcaaatgaa actcgattcg ctgaaataaa
     2161 tgccgtctga aatgccgtcc attatttatc agacggcatt ttaccctccc tctttatggc
     2221 ctcttgccgt gaagaaccta tttaccgtgt ccaacggaca cctgctcgtg ttcagttctc
     2281 ccgaacggca cttgacacag aaagactcaa tcatgaaacc aaccatcgcg cttgtcggcc
     2341 gccccaacgt cggcaaatct actttattca accgtttgac gcgcaccaaa gacgcactcg
     2401 tacacgacct gcccggcctg acccgcgacc gccattacgg acacggcaaa gtcggcagca
     2461 aaccttattt ggtcatcgat accggcggtt tcgagccggt tgtggacagc ggcattttgc
     2521 acgaaatggc aaaacaaacc ttacaggctg tcgatgaagc cgatgcggtt gtgtttttgg
     2581 tggacggccg taccggtctg acgccgcaag ataaaatcat tgccgaccgt ttgcgccaaa
     2641 gcccgcgccc cgtttatttg gccgtgaata aaggagaagg cggcaataga gccgtgcttg
     2701 ccgccgagtt ctacgaactt gctttgggcg acccttatgt tatttcaggt gcgcacggtg
     2761 acggcgtgta ttatctgatt gaagatattt tggaaacctt ccctgagcct gaaaaagaag
     2821 aggaagaggc gaaacatcct gtttttgccg ttatcggtcg tccaaatgtc ggtaaatcaa
     2881 ccttggtaaa cgccattctc ggcgaagagc gcgtcatcgc cttcgatatg gcaggtacga
     2941 cgcgcgacag tatccatatc gatttcgagc gcgaaggcaa accgtttacc atcatcgata
     3001 ccgcaggtgt gcgccgtcgc ggcaaagtgg atgaagcagt ggaaaaattc tccgttatca
     3061 aagcgatgca ggcggttgaa gcggcaaacg tcgctgtttt ggtattggac gcgcagcagg
     3121 acatcgccga ccaagatgcg acgattgcag gtttcgcttt ggaggcaggc cgtgctttgg
     3181 tggtggcggt caataaatgg gacggcatca gcgaagaacg tcgcgagcaa gtgaaacgcg
     3241 atatcaaccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt tccgcattga
     3301 aagagcgcgg tatagacggt ttgtttgaca gcattcaggc tgcctacaac gcggcgatga
     3361 ttaagatgcc gacgccgaaa atcacgcgcg tattacaaag cgcgatcgag cgtcagcaac
     3421 cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc ggcatgaacc
     3481 ctcccgtaat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc tatacgcgct
     3541 atctgaccca aacgttccgc aaagccttca atctgcaagg cacgccgctc agaattcaat
     3601 acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa aaaccgctgc
     3661 gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgcaaagaa gagaaaaacc
     3721 gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa ttcccgacat
     3781 ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg ctcaacaaag
     3841 aaaagctgcc tgaggcagac gtacttgccg acaaacttgc tgaagctgaa tttacccatt
     3901 gccaaggctt agagtggttc agcgaaggct ttgccgcacc ggtttcgttc tctcccgaac
     3961 tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaagaa aaagtcctgc
     4021 ctgccggcgt catccgcgat attttggaag agaaggtggc ggaaatccaa aacaatgaag
     4081 cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca gacgacctgc
     4141 tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc cgccacggct
     4201 acctgctcgt caataacgcg gcttccgcca aagcagaaaa catcctgacc aagctgcgcg
     4261 aagctttggg cggtttggaa gcctcgctgc cgaataccaa acaatcgccc tcttctctga
     4321 tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattggac agcgattgcg
     4381 aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa gatttaaccg
     4441 ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt aacccagctc ggtttggttt
     4501 ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc atccaatacc
     4561 tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccagc cttgccttcg
     4621 cctcgcaaat cctgatggct gaatccgtca gcaccatgtt ggaagagctg gtttcctatt
     4681 tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca aaatccaatg
     4741 gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg ttcgccctcc
     4801 ccgccgcgtc cgccgttctg acttcctatc aagaacccgg ctgcacctac gacggcggag
     4861 tcggaaaaga caggcttcct tcaggcaaag gcatatggcg ttgccgggac gggcgcggtt
     4921 ataccggttc gtttaaaaat ggcaaattcg acggacaagg cgtttatacc gttgccgcca
     4981 accgcgaaat atttatcgaa ccgttcaatt ccgacagtac caaattccgc aacatggtac
     5041 tctcgggcac gttcaaaaaa ggcttggcac acggcagatt taccgtctcg caaaacggcg
     5101 aaaccctctt cattatgaaa tgcgaaaacg gcatgattaa agaagtgaag ctgcccaaaa
     5161 acaaataagc cccgttttca gacggcatcc cccaatgctt tgtccgaaca ctgcaagaaa
     5221 caaggaaaac attatgagca tcaagtccga caaatggata cgccgcatga gcgaagaatt
     5281 cggcatgatc gaccctttcg agccgaacca aatcaaagaa gccgacggca aacgcatcat
     5341 ctcctacggt acgtccagct acggctacga catccgctgc gccaacgaat ttaaaatctt
     5401 caccaacatc aacagcacca tcgtcgatcc caaaaacttc gacccaaaaa acttcgttac
     5461 cgttgaagac gactgctgca tcatcccgcc taattccttc gcactggcgc gcacggtcga
     5521 atatttccgc atcccgcgca acgtcctgac cgtctgcttg ggcaaatcca cctacgcccg
     5581 ctgcggcatc atcgtcaacg ttaccccgtt cgaaccagaa tgggaaggct acgttaccct
     5641 cgaattttcc aacaccaccc ccctgcccgc caaaatctac gccggcgaag gcgtggcgca
     5701 agtcctcttc ttcgagagcg acgaaatctg cgaaacctca tacaaagacc gcaacggcaa
     5761 atacatggga caaaccggcg taaccctgcc caaagcctga aatataaatg ccgtctgaac
     5821 ccttatcgtc gggtttcaga cggcatttgc cgttcacctc aaatcagaac tgaaccgtcc
     5881 cattgacgct gatgctgatt tcctccacac ccggcgcggc ggaatccgta ccctccacgc
     5941 cgtaacttgc cgccatcggc atggcacgaa gcatttttgc ctgagcagct ccttcacccg
     6001 cgatatggct gccgatgtgt cccaaattta atttgacgat tttataaccg gacgtaccca
     6061 atatgcccga caacttgtct gcacgcgcct tgaaacgcaa aatagcatct ttactgacct
     6121 gatcgatgac ctcgtttcgg cgttcgcgcg acacatggaa atccgtatat tccaatgcgg
     6181 catctgcctg aacatcggca ataaaacggt ttaactcatc aaaatccctc ccttcgacct
     6241 taaattccgc acgctcctcc caacctgttt gaatgcgtct gccgttggta tattgatagc
     6301 gcggcatcgc actgcgcgat accaattcgg ttttaaagct accatttttc gattttctga
     6361 tgaacttgtt gaatttttta acaaactcgg cattgacggc atttttgtcc cgtccttccg
     6421 ccgccacctg taaacgcgcg gacattgtat cctgaaccgc ctcgacaccg gccgattcgg
     6481 aaaattcgac aatattgtaa ttcaatgctt cagccgctgc cggaaaagat accgccaata
     6541 gggaagccgc caaaatagaa cgcagcatat ccgtatcctt acttataaaa ccgccaatct
     6601 agcatatcgg cgcgctaaat tgcgttagcg taaggcaaca atttaaatat tcggtctgcg
     6661 tctgtcttat aataaccgcc tttccgatac ggaatcccac tatgcccaaa cccttgcacg
     6721 tcctcgtcat cccctcatgg tatccgcaat ccgaacagga tgtggacggg atttttttca
     6781 aaatcaggca ctggcattgc agaggaaagg catcaaaacc gccgtgcttg caccgatgtt
     6841 ccgctacttg cggaaagaaa cagcaagcat cctgaccgac cttacggttt tgcaaaatat
     6901 cggcaaagcg gtttggacat ctatgcatgg cacggcatgt atttcttccc ccgctttccg
     6961 ttcatcgaca tcgaccgcat ccgctgggtg cgcgtgggtt tgaaagcctt caaacactac
     7021 atccgcgaaa acgggctgcc cgacctgatt cacgcccacc gcatgaacta tgccggcata
     7081 ctcgcccaaa aaatttccca aaaatacggc atcccctatg tcgtcacgaa acacagcagc
     7141 accattacgc gcggtctgat ccgtccccac caatggcagc ctatgaaaaa tgcggcggcg
     7201 cacgccgccg cacgtctcgc catcagccgc catttcgcac acgtcctgca acacaaatac
     7261 ggctgcgaat ggcagtacct ccccaacata ctgggcggaa tattcaaccg accgtttgaa
     7321 caaagagaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
     7381 ctcaaaggac acgatgtcct gctcactgcc tttgcccggg cgttggcaca atgcccgcaa
     7441 ctgcgtctaa acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
     7501 gacttgggca ttacccatgc cgttacattt ttgggcgcac tgcaacccga agcagtcttg
     7561 gatttgatga agaacagcga cgcatttgtc cttgccagcc gcaccgaaac cttcggcgtg
     7621 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
     7681 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
     7741 gccgacgcac tcatcaaaat gtatgaacac cactctgatt tcgaacctgc ccgacttagg
     7801 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
     7861 cgacaggcaa tcgcagaata cggtaagaaa ataccggtga aatatagtgg attaacaaaa
     7921 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggaaccga ttcacttggt
     7981 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
     8041 taaatctaat ccactataga ttaaaatcac acaatatcca actattcgga ccttcatatg
     8101 acacataccg tccacctgca tttagaagaa accgacaact tggcgttgca gcgtctgtgc
     8161 ggttcttttg acaacaacct tgatttactt gctaaagcac tcgatatcca catcagccgc
     8221 cgttttgaac atttcacttt caacggcgca tttgcacacg ccggcaaacg cgcactgctc
     8281 aaactcttgg aaacggcgca gacgcgcgac ctaaacgacg gcgacatcag gcttgccgcc
     8341 gtcgaagccc aaaccgaaga tgccggtcat caagaaaaaa accatgacca cgcctattat
     8401 ttccgtacca aacgcggcag catcggcggc agaacgccac ggcagaacgg ctatatccgc
     8461 gccctactca accacgacat cgtattcggt ctcgggccgg caggtacggg caaaacctat
     8521 ctcgccgtcg ccgccgccgt cgatgcgatg gaaaaacacc aagtcgaacg catcatttta
     8581 gtgcggcctg ccgtcgaagc cggcgagaaa ctgggttttc tgcccggtga cctgacccag
     8641 aaagtcgatc cctaccttcg tccgctttat gatgccctct atgacctgat gggctttgac
     8701 cgtgtcacca agctgattga aaaaggcctg attgaaatcg ccccgctcgc ctatatgcgc
     8761 ggcaggacgc tcaacggcgc atacatcatc ctcgacgaag cgcaaaacac cacgcccgaa
     8821 caaatgaaaa tgttcctgac ccgcatcggc ttcggcgcga aagccgtcat taccggcgac
     8881 accagccaaa tcgacctgcc caaaaacatc aaatcgggat taaaagatgc gcgtgagaaa
     8941 ctgcacggcg tggaagggct gtatttccac acctttaccg gcgaagacgt tgtccggcat
     9001 cctttggtgc aaaaaatcgt cgaagcctac gaatcggcag aacacgactg acattccaat
     9061 gccgtctgaa acccgatgca tggaaaatcg ggcagagaaa aacagatacg gaagccaagc
     9121 tccgtatccg tatccgtttc tttatccgtc caaccgatac atcaatcgaa ttttctgcat
     9181 cggattgcct catcttcgca aaacattgcc gaagtttcgc ggttgcacgc ctgacgcact
     9241 ttctgccgcg ccacgccttc attccgatcg cttgcctcaa acgtcttctg aaaaggcgta
     9301 acgctgcata catacatggt ggaatttgaa ccgaaaaggt cgattttgac ggtattgctg
     9361 ccgctttcca atacggcgac acggtgttcc aactgcgcca gacgtgcttc caaatcgccg
     9421 attctgtcgg caaatgcagg catggcggca cacgcggtca aaatgcccaa aatacatttt
     9481 ttcatcaatt tttccttgtt tcttgaaccc tgcccacccg gacatccttt ttccgagagg
     9541 cagggttaga ctttatttac ggggatgaat ccccttacaa atcaggataa tacatagaac
     9601 ggcactttat gcttcatccc atgtgttgaa acttcattgc tccttgtctg ttgaccctta
     9661 ttcccaatcc gttcccacaa ccttcggctt gggtttgcgc ctgcgacggc ggcgtttttt
     9721 cgcctgtcct tcgttttttt cgtgtcgggc ggcggcttcg tttttggtca tctccgaccg
     9781 ctgttccgtc gatgccgtct gaaacgccgt ccaccactcg gcaagggcgc ggtccgcatt
     9841 gccggtttcg gcgcgcaaga gcaggaaatc ataggcggca cggaaacgcg cctgtgcaaa
     9901 cagtttgtgc ggacgtgcgc ctttgcggtt ttcaaactgc ggctggaaca tccaaatttc
     9961 gcgcatcgtg gcggaaaagc gttgcggcac gccccaaccg cgttcgacgg tttcgcgcat
    10021 cgtattgatt gcatcggaca gggcgggcgc gggtttcaaa ccctgttgca gattgctttt
    10081 ccaatggcgt tccaactcgg gccacatcag agccgccaat acaaaaccga ccgaaaccga
    10141 tttgtcggca cgcagccgct catcggtatt tttcagggca agcaccgtca tttttccggc
    10201 gatgccgtct gaaacacgca aggcattgag cagcggatgg atgtcgtccg gtatgtcaaa
    10261 tccgttcaaa cgtttcagac actcgcgcgc gtgccctgaa aacagcagtt tcatgatttc
    10321 gtcaaacagc ctcgctaccg gttcgtgctt cagacggcat atcgattcgg caatcggtgc
    10381 ggtggtttct tccgacagct caaagcccaa tttgcccgac aggcggatgg cgcgcaaaat
    10441 cctgacaggg tcttcctgat agcgttcggc ggcatcgcca atcataacca gcctgcgggc
    10501 ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct tctttttccg gatcgtaata
    10561 caaggcattg caggtaaaat cgcgccgcat cgcatcttct tcgatgctgc cgtaggtgtt
    10621 gtccttcata atcctgccgt gcgcgttctg atgaactttc gcaccgccgc gaaacgtcgt
    10681 tacttcgata atctccgcac cgttcatcac atggacgatt tgaaaacgcc tgccgacgat
    10741 gcggctgcgg cggaagagtt tgtgcacctg ttcgggcatg gcatcggttg cgacatcgaa
    10801 atccttgggt tcgatgccga gcagcaggtc cctgatcgcg ccgccgacca cataagcctg
    10861 aaaccctgcc tctttcaggc ggcgtatgac gttttcggcg gcaaagctca acatttcggc
    10921 acggatgttg tgtctttcgg caggaatgac cgttttactt tccgcttctt tactgctccg
    10981 actggaaggc agcatcttat tcaaccattt tttcagcata aatattctga cccgaagagc
    11041 ggcatctcca cgggcaaccg tgttcagacg gcatcgggcg caggtttgca agatgccgta
    11101 accataaaca aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcaa
    11161 cgcgtcatta taacagattg ccctccaaaa aaatcagacc ctgcttttgt ggcagagtct
    11221 gaaatttgat gcaccttata tattgcgtcc ctaagaaggg atgattaaca aaaattaacg
    11281 ccctttactt tcttttccga ttgggaacat tcccgtcgta caaacaggca aatgaaggaa
    11341 cagaaatcac attcaaacgg cgttccgccg cctctacgcc gcttcccagt agtcgatata
    11401 aagctgcaat tcgaggttgt tgcgccattc gttggcaacg gggcggtaca ccgtgcggat
    11461 gtattcggga atgtcttcgc tgcaccgcca gaacattgct tcaaattcgc agccgtcttt
    11521 ttgcagccac gcttttttgt gtttgccttc cgcgcccaaa ggctgctggc ggacgacgtg
    11581 gaactcgtcg gtaaaactcg gcggcgcgaa gccctgcccc caaacgtgtt tagcaaggtt
    11641 ttgcgcctgt tccagcgtaa tgtcgcgtgc aggcaggctg ccgtcggtga tgaaggtttg
    11701 cgacaaatcg tcttcgcaca ccatttcgcg cacggcttct tcaaaggccg tctgaaacgc
    11761 gggaatgttg tgttcgagta tgctcaagcc cgccgccatc gcgtgtccgc cgaatttcaa
    11821 aatcaaatcg ggatggcgtt tggacaccaa gtccaaagca tcgcgcaagt gaagattggg
    11881 aatggaacgc cccgaaccgc gcacttcacc gttgtcggca ggcgcaaaca cgatggtcgg
    11941 acgataaaaa cggtctttga ggcggctggc aacgatgccg accacgcctt gatggaagtc
    12001 gtcgcgatac gccaccaaag tcgtctgacc tgaaggcagg gtttcaggaa aagcattcag
    12061 cgcgtcttgc agcatagact gctcgatttc gcgacgttcg atattgaggt tgtttaactg
    12121 cgccgccagt tcctgcgcct cggcatcatc tcgcgccaac agacaggcaa taccgaccga
    12181 catatcgtcc agccgtccgg cggcgttgat gcgcggcccc aacgcaaaac ccatatcaaa
    12241 cggctgcgcc ttgcgccaat cgcgccgcgc cacttcaaac aaggcgcgga taccggggcg
    12301 cattttgccg gagcgcatcc gtttcaaacc ttgcgacacg aggatgcggt tgttgtggtc
    12361 gagagggacg acatcggcaa ccgtgccgag tgcgaccaaa tccaaaagtt cgcccaaatt
    12421 cggctctttg atgccgtctg aaaaataatt gcggcggcgc aattcggcac gcaacgccat
    12481 caatacataa aaaatcacgc ccacgcccgc caagcttttg cttggaaaac cgcagccttt
    12541 ttggttcgga ttgacgatga tgcagtcggg cacggtctcg gcaggcagat ggtggtcggt
    12601 aacgatgaca tccaaaccca gagcctgcgc cctcgccaca cctgcgatgc tggcgatacc
    12661 gttatcaacc gtaatcagca aatccacacc ttgctctgcc gcgatttcag ccagctcggg
    12721 cgttaagccg tagccgtgct caaagcggtt gggcacaagg aaatccactt tcgcccccat
    12781 cgccgccaaa ccgctcatac cgacggaaca cgccgtcgcg ccgtcggcat catagtcggc
    12841 gacaatcagg attttttcct tgcgttcaac cgcatccgcc aaacggccgg cggcggcttc
    12901 gcagtttgtc agcgtttgat aaggcaggag ggaagcgagt ttgtcgtcca attcggcagg
    12961 actttgcaca ccgcgcgagg cacaaagccg ggcgattaaa ggatcggcac cggcggtgag
    13021 caaatgatta aaaacgtcgg tattgacgga tcgggtttgg attttgactg acatgacgaa
    13081 gttttttcaa gatatgatga ggctgaaatg ccgtctgaaa gcttttcaga cggcattgct
    13141 tttcatttgc gggcggcatc cacgaccgcg cccagagccg ccgcaggcaa agccagtacc
    13201 gcacccgccg catccagtat caacaagggg ggcgtatata agatatttcc aaacagcttg
    13261 gatttgtcgg tatgtttttc agtaaccgtg taataaatat cgacaggcac actctgttca
    13321 aaatggtaat cggcgttcag tttttgcggt gtggcgtagt atttgccttt ggcggatacg
    13381 cagcgcgtgt aaatagtccg attgtcaagt tcgaccgctt caaactcaag ctgtttcagc
    13441 ttggcgatgt cagcaggcct gtcggtatcg tagcgcaggc aaaggccttc ggtactgaaa
    13501 ttctggctgc caggcgattc gagtttgacc ggcagggctt ggtggcgagc atagctcggg
    13561 gtatcctcaa ctatttggaa gggtttgtcc aacccggcct tcaaaatgcc cgtcagcttc
    13621 gccgaatctt cgggattgac gacgaaccag tatttcccgc ccatcatcac cagactgccc
    13681 ttttccaatt gggcattgtc tttggcaacc acaccgaagg cgcggatttg atccttgtca
    13741 acgtgtttat aggcggtcgt ttggctgaac gggtcgttca tgccccacat cattgccgta
    13801 cagccgttca acatcaaggc tgccgccaaa acggcggcgg tcgttttacg gaacaacatt
    13861 ttatcctcca atctaaataa cggcggatgt cgtctgaaaa tatgcctaaa tttccgcgtc
    13921 cccgttttcc ataacggttt tcaactcttg aaacagggcg cggaaatttt tcggcggttt
    13981 gttctgttcc tgttcttttt tggtattgcg gatgagcgtc ctcagcttgc ccgcatcggc
    14041 gtttggaaaa tcggacataa actgcgtcag cgcgccgtcg tccgccagca gctccatccg
    14101 cgtccgctcc acacgttgca aaaaggcgtt gtgcgccgca tcgtcgccgc gcagcttggc
    14161 aagaaatgcc tcgatgggcg cgggatcggt atcgcgcatc agtctgccga taaattgcgc
    14221 ctgccgcttg agcgcgccgt tggatgtgat ttttttatag gtaacgatgg cttcgtacaa
    14281 atcttcgtcc aagccgattt ttttcaacgt gtcgcttgag agcttggtca gctccatacc
    14341 caaatcctgc aaaccgttca tctgcttttt catttgggtt ttgctgaccc attcgtcttc
    14401 ttgttcaaac atcttcattc tcaaatttta tccgacgggc gcattttaac agaatgattc
    14461 cgcgttcaga cggcattttg caccgtccgc cctgcaaacg gacgcttttg cccctgccgc
    14521 ccgaaacggt taaaatgccg caacgctcca ccaagaaaga tttctatgct gttcaaccac
    14581 accgcttccg aacttctcga cctctgccgc cgcacgctcg acttggcaaa agcagcgggc
    14641 gcgaccgccg ccgaagccga tttcagcgaa tccatcggac aaagcgtcag cgtgcggctg
    14701 ggcgaaatcg aacaaatcga gttccagcag gacaagtcgc tggacattac cgtttacgtc
    14761 ggcaaacgca aaggccgcgc tggcaccgcc gatttctccg aacaagccct gcgcgacacc
    14821 gtccgcgccg ccgccgacat cgcccgccac accgccgaag acggttgcgc cggacttgcc
    14881 gatgcctgtc tgatggcaaa acacatcggc gaccccgacc tttaccacga atgggatttg
    14941 gatacggaag ccgccgtcgg cttggcaaaa caatgcgaac aagccgccct gaacgaggat
    15001 gagcgcatcg aaaactccga aggcgcggcg gtgcaaaccg gccattacca atacgtttac
    15061 ggcaacaccc acggttttgc cgcacaccgg caaggcacgc accacagcat ttcctgcagc
    15121 gtcgttgccg ccgacgaaaa cggcatgcag cgcgactact ggtacgattc cgcctgccgc
    15181 catccggata tggacagccc cgaaaccatc ggtcaaaccg ccgcccgccg cactttgcgc
    15241 cgactcggca gccgcagcat cccgacaggc agctaccccg tcctcttcga taccaccgtt
    15301 tcgggcggtc ttatcggaca cctcgtcggc gcactctccg gcggtgcgct ctaccgccaa
    15361 agcagtttcc tgatcgacag catcggcaaa aaagtcctgc ccgatttcct caacctgcgc
    15421 gaagaaccgc acatcccccg ctctttccgc agcagctatt ttgatgcgga aggcgttgcc
    15481 accgcgccgc gcttcgtgat tcaaaacggc attgtcgaag gctatttcct cagcagttac
    15541 agcgcgcgca aactcggtat gcagaccacg ggcaacgccg gcggcgcgca caacctgtat
    15601 ttgaaccata cgcacgaaac gcaatccgac ctgctgaaag aaatggggac gggattgttg
    15661 gtaaccgaac tgatgggaca gggcgcgaac accattaccg gtgactactc gcgcggcgcg
    15721 gcgggttttt gggtggaaaa cggcgtgatt gcctaccccg tccacgagat taccgtagcc
    15781 ggacgcttgc aggatatgta ccgcgacatt gtcggcgtgg cggatgacgc tttgcggcgt
    15841 tcgtccaaca aaatcggctc gattctgatt gcggggatga cggttgccgg aagctgaacg
    15901 ccctgccgat gccgtctgaa gacgaaggca gcacgttttg ccccgtgcct gcccgtttgc
    15961 cgttttatgc cgcaccgcct tttgccgaca acattgcaca ttttttcatc agcactgatt
    16021 ttcccttatc cgtcaaaata ctaagccact tgtccacaac agggtacaca caagttcagg
    16081 ataaggcgtg cggggattag ggtttttccg ccgccggcaa aacggcagac aaaaacggcg
    16141 ggacattgcc tgtcccgccg ttttcaaata tacatttgac cgtacatcag gaattaagca
    16201 gcctgaatgt tggcggcctg tttgccttta gggccggtgg ttacgtcgaa agagacgcgt
    16261 tggccttctt tcagggtttt gaaaccttcc atattgattg ctgagaagtg agcgaacaaa
    16321 tcttcgccgc cttcatcagg agtgatgaaa ccaaaacctt tagcgtcgtt aaaccatttt
    16381 acgataccgg ttgccattag aaacttccta tactcaaaaa ttaacaaaat cagcaaaaca
    16441 aggcatacgg caaaccgagc cgtctagcgt tccttccagc ttgatatact cggcggatgc
    16501 ccaatcctgc ttaaccgtct ttttacattt gttaagacaa aacgtcaagc tatgttttca
    16561 aaaaagtggg aaaataggca aaagtaccga aaatacaaca aaaatgccgt ctgaacgcgg
    16621 ctttcaaatg ggacacacta tgaccgcgca acaccaatcc gacacgctct tgcaccgtct
    16681 gaacaccctg ccgccgaaac gttacggcgt tttcctattg aacgacgatt acaccacgat
    16741 ggaatttgtc gtcgaaatcc tgaccgaaat ctttatgctc ggacaagaac aggcggtagc
    16801 ggtgatgctc ttggttcatc acgaaggcaa aggcctgtgc ggcacttaca cgcgcgatat
    16861 tgcccaaacc aaacaacaac aagtcatgca gcgggcaaaa gccgaagggc atccgctgca
    16921 atgtattgtc gaggagattt aatatgcttg cacccgaatt ggaacagatt ttgcagcagc
    16981 tttaccgcga ggcgcgtaag gctcattatg aatttatcag cctcgagcat ctgcttttgg
    17041 tactcatcga agaagatgcc tccgtgccga acgtcttaaa actctgcggc gcggatttga
    17101 aagtggtgtc cgaacagctc gccgccagcg ttgccgaaaa cacccccctg attcccgaac
    17161 accttttaga cacggtcgaa acccggccca cgctcggctt ccaacgcgtg atgcaacggg
    17221 cgatggtgca tacccagtct gccggaaaag ccgcagtcga accgttggac gttttggtcg
    17281 cgctgatgag cgaaaccgac agccacaccg tctatttcct caagctgcaa tcggttacgc
    17341 gttttgaagt tttgcgctgt attgcccacg gctctcccga tgaagatgaa gacgatggca
    17401 actattcttc agacggcatg gacgacgata atgaaaaccg caccaaaccg ggcaaaaacc
    17461 ctttatcggc gtacaccgtc aacctcaacg ccgaagtcaa agccggccgt atcgaccctt
    17521 tgattggtcg caaacacgaa atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa
    17581 acaatccgct tttggtcggc gaagcgggcg tgggcaaaac cgcgctggcg gaaggtttgg
    17641 cacatcaaat cgtcaacggc ggcattccag acgcgctcaa agatgccgaa gtgtacgcgc
    17701 tggatatggg ctcgctgttg gcgggcacga aataccgcgg cgactttgaa gcgcgggtca
    17761 aatccgtctt gaaacagctc gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc
    17821 acaccatcat cggcgcaggc agcaccagcg gcggcaccat ggacgcgtcc aacctgctca
    17881 aacccgcgct ggcaaaaggt tcgctgcgct gcatcggcgc gaccacttac gacgaatacc
    17941 gcaccatttt cgacaaagac cacgccttaa gccgccgctt ccaaaaaatc gacgtggtcg
    18001 aacccaccgt ttccgaaacc gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct
    18061 tccaccaagt ccgctatacg caaggcgcac tcgaagccgc cgccgaactc tccgcacgct
    18121 acatcaacga gcgtttcctg cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg
    18181 cgcaacggat tctgcccaaa tccaaacaga aaaaagtcat cggcaaagca caaattgaaa
    18241 ccgtcatcgc caaagttgcg cggattcccg aaaaaaccgt gtcgcacgac gacaaacaag
    18301 tgctgcaatt cctcggtcgc gatttgaaaa acatggttta cggtcaagaa aacgccatcg
    18361 acgcgctggt tgccgccgtc aaaatgtcgc gttccggcct tgccctgccc gacaaaccga
    18421 taggcagctt cctcttctcc ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc
    18481 ttgcctactc gctgggcgta ccgctgcaac gctttgatat gtccgaatac atggaacgcc
    18541 atgccgtatc gcgcctcatc ggcgcgccac cgggctacgt cggctttgaa caaggcggcc
    18601 ttttgaccga agccgtcaac aaacagccgc attgcgtatt gctcttggac gaaatcgaaa
    18661 aagcccaccc cgacattttc aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg
    18721 acaacaacgg caagagtgcc gatttccgca acgtcatcct gattatgacc accaacgcag
    18781 gcgcggaaag cctcagccgc cccagcctcg gctttaccgc caagcgcgaa cgcggcgacg
    18841 aaatgcaggc aatcaacaag ctcttcacgc ccgagttccg caaccgcttg gacgcgatta
    18901 tcccgtttgc acccttgtcc gaacccatca tcgtcaaagt cgtggacaaa ttcctgctcc
    18961 agctcgaaca ccagctcctc gacaaaaaag tcgaagccga attcacgtcg gcattgcaca
    19021 aatatctggc ggaaaaaggt ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga
    19081 ttcaggaaaa aatccgcaaa ccgctcgccg acgaactcct gttcggcaaa ctatccgacg
    19141 gcggcttcgt gcggatagac tgggatgcgg caaaagaaga agccgtgttg aagtttaaga
    19201 aaagcaaggt caaaataaaa accgcctccg cataaaatca aaaatgccgt ctgaaaggtt
    19261 cagacggcat tttgtcaaat atcggtccct attccggcaa acttctccac aaacgccgca
    19321 atcttttgaa aaacactttg tttcttcgtc agatattgcg ggttgagcgg actcattttc
    19381 ggcagggttt cggtcagttc cgtaccgttt tcgctggcat agccgcgttt gagcgagccg
    19441 gtcaaatagc gtttggcggc ggtttcgttc aggtcttcgg cggcaatcaa tcctgccgct
    19501 tcgtgccgca ttacctcttg cgcgtaggtg taaaaggttt ccagtatggc gggaacgtcg
    19561 gggactttat ccaaatccgt atcgttgata aaatccacaa tcagaccctc tttggcgcgg
    19621 tggccgatgc tggcgcggat gatgcggcgg atttcttcca ccagctccgc ttttcctttg
    19681 atttttttgt gatgctcgaa aaccagttgc aagatgtaat ccaaattgat ttcctgcgat
    19741 ttgagcaaat ccacctcaaa aaccacatcg tcccaatcga tttttgactg ttctttctgt
    19801 tcgcctgctt tttggcggct cagccagtct cggatgtcgt tgtaggcgga acggtagtcc
    19861 tgcaccgccc tttcagacgg catcggcact tgccgcattt cctgcacgtc ttcatcgctc
    19921 aggtagtatt tttcctgaaa cgccttcatc gcatcttcgt ccgccgcatc cacattctgc
    19981 aactcgcgca gcgcggcaaa ttcatcgtag ttctgcaata cgttttccgc ccgcaggtat
    20041 tcgccgaaga gtttggcaaa atcttttttg tctttttccg tttcgatttc gtcgggatcg
    20101 gggaaacgtt cgcgcaattc ttttgccaca tccaaatagc cgcgccgcgc ttcgccggtc
    20161 tggctgtcga tatagccgtt catgtattct tcgtagcttt tttccagcac cacgtttttg
    20221 gtgtttttgt cgccgaacag ggtaatcgca tcaatggtcg cctgctccaa atcgcggaag
    20281 cagacaatat tgccgaaggt tttggtggca tcgtaaatgc ggttggtgcg agaaaacgcc
    20341 tgcatcaggc cgtgatagcg caggttttta tcgacaaaca gcgtgttcag cgtcggcgcg
    20401 tcaaaacccg tcaaaaacat gccgaccaca atcagcaaat ctatttcctg atttttcacc
    20461 cgttttgcca aatcgcggta gtagttctga aaggctttgc tgtccgtgcc gaaattggtt
    20521 ttgaaacagg cgttgtaatc gttgatggca gcctgcaaaa attctttcgc gctgctgtcc
    20581 atcgcttccg gttcaaaggt ttcatcgaca atttcaccga cggcgttctg ctcttcgttg
    20641 gcagcaaagg aaaaaatagt ggccactttc aacgggtgca ggctgcctgc ctgttgcgtt
    20701 ttgaacgctt cgtaatagca cttcgccgca tccacgctgc tgacggcaaa catcgcgtta
    20761 aagcctttgc cgcccgcatt cagccggtgc gttttctgcc tgaactggtt caggatatat
    20821 tgcgtgattt cgcggatgcg ttcggggtgc agcagggctt ggcggttttc ggcggcactc
    20881 agtttcttct cgtcctgttc cgcttccacg gctttgaact gcgggcgcac atcgttgtaa
    20941 tccactttga atttcaatac tttttcatcg cggatggcat cggtaatcac ataagaatgc
    21001 agctcccgcc cgaacacgcc cgccgtggtt tccgcgccca aagcgttttc gggaaaaatc
    21061 ggcgtgccgg taaaaccgaa ctggcagaat tttttaaatt tctttttcag gtttttttgc
    21121 gcttcgccga attgcgagcg gtggcattcg tcgaaaataa agacaacccg ctgatggtaa
    21181 accggcagat tatcttcact tttcatcagg ttgttcagct tttggatggt ggtaacgatg
    21241 attttgttgt cgtctttttc caaattgcgt ttcaagcccg ccgtgctttc cgaaccgttc
    21301 acgctgtcgg tcgaaaaacg ttggtattcc ttcatcgtct gatagtccaa atccttcctg
    21361 tccaccacga agaaaacctt gtcgataaat gccgattccg tcgccagacg cgccgcctta
    21421 aagctggtca gcgttttgcc gctgcccgtg gtgtgccaaa catagccgcc gctctccggc
    21481 ttgctccaat tcttcgcctg cgccgagctg ttgattttcc acagaatgcg ttcggcggcg
    21541 gcaatctggt acggccgcat aatcagcagc gtatcattcg catcgaacac gctgtaatgc
    21601 agcaaaacgc tcagcaatac gcttttttgc aggaacgtgg cggtaaagtc tttcaaatcc
    21661 ttaatcggat gattgtccga ccgcgcccaa ttcatcgtga aatcgaagct gtttttgtcg
    21721 cgcttggtgg tgttggcgaa atagcgcgtg tccgtgccgt tggaaatcac gaagatttgc
    21781 aggaatttga acagcgaatt ttcgctgttg aagctctctt tgctgtaacg gtgcacctga
    21841 ttgaatgctt cgcgcaccgc cacaccgcgc tttttcaatt caatctgcac cagcggcaga
    21901 ccgtttacca acacggtaac gtcataacgg tttgcatgcg tgcccgtctg ctcaaactgg
    21961 ttgataagct gcacatggtt gcgggcaagg tttttcttgt ccagcagata aatgtttttc
    22021 agacggccgt catcaaaagc gaaatcgtaa atatggtcgt cgtggatttt gcgggtttta
    22081 tcggtaatat tttcagacgg tctgtccaga tattccgtca aaaaccgcgc ccattcgccg
    22141 tctgaaaacg ccacatcgtt cagccgctgc aactgcgcgc gcaggttttc cagcagcctg
    22201 ctttggctgt tcaaatcctt gcggtattcg taaccctgat tctgcaaatc ggcgattaac
    22261 tccgcttcca accggttttc cgattggtag ctgcccgact gttcgatttt ttcatattgg
    22321 tcgagcacga tgaaattcgg cgtttcggcg atgggtttgg tttcgaggtt catgggtcgt
    22381 cctttgcagt atcgggtttt aaacggtttg atgttcggat tgggctgcct gaaaatgtgc
    22441 aggctgcttt ttcaggtagc cttagggaag gcaagcagct gttcgcggta atattcgtat
    22501 tgtttgcggc gcagggcgat ttcgtgcggc aggccttcgc tgatagagtg ggtcaggata
    22561 tcgaatttgt ctaggatggc ggcaattttt tcctgttcgg ggagtggtgg gatggggatt
    22621 tttaatttct ttaattcttt catgctaata ataggtaaac ttgaagtatt aataagtttt
    22681 ttgctctgct ccgcaataat gaaaaaataa tagaaagcga aatttatatc taaagcatcc
    22741 ttaaattcat ctttaattgt aaaattggtc agttgttggt tactaatata attaa
//
LOCUS       DAXIHS010000026        22265 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXIHS010000026 DAXIHS010000000
VERSION     DAXIHS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.026"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(72..665)
                     /gene="ribA"
                     /locus_tag="ACFOF2_000891"
     CDS             complement(72..665)
                     /gene="ribA"
                     /locus_tag="ACFOF2_000891"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC7107237.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(658..1332)
                     /locus_tag="ACFOF2_000892"
     CDS             complement(658..1332)
                     /locus_tag="ACFOF2_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107238.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1486..1570
                     /locus_tag="ACFOF2_000893"
     tRNA            1486..1570
                     /locus_tag="ACFOF2_000893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1520..1522,aa:Leu,seq:tag)
     gene            complement(1661..1849)
                     /locus_tag="ACFOF2_000894"
     CDS             complement(1661..1849)
                     /locus_tag="ACFOF2_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107239.1"
                     /translation="MADERKGFFDAIIKINIFFFPVETPALGAVNLIAGGSTRFRFGA
                     EQMAALYVPFCVLKHIGR"
     gene            2090..3124
                     /gene="purM"
                     /locus_tag="ACFOF2_000895"
     CDS             2090..3124
                     /gene="purM"
                     /locus_tag="ACFOF2_000895"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC7107240.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(3598..4254)
                     /locus_tag="ACFOF2_000896"
     CDS             complement(3598..4254)
                     /locus_tag="ACFOF2_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC7107241.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPMLE
                     "
     gene            complement(4251..6023)
                     /locus_tag="ACFOF2_000897"
     CDS             complement(4251..6023)
                     /locus_tag="ACFOF2_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC7107242.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQPQQGTTVSLTGASEESLK"
     gene            6229..6843
                     /locus_tag="ACFOF2_000898"
     CDS             6229..6843
                     /locus_tag="ACFOF2_000898"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC7107243.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            7006..7623
                     /locus_tag="ACFOF2_000899"
     CDS             7006..7623
                     /locus_tag="ACFOF2_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC7107244.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKSNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            7631..8494
                     /locus_tag="ACFOF2_000900"
     CDS             7631..8494
                     /locus_tag="ACFOF2_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC7107245.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYANKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(8507..8941)
                     /locus_tag="ACFOF2_000901"
     CDS             complement(8507..8941)
                     /locus_tag="ACFOF2_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC7107246.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            9252..9980
                     /gene="rpe"
                     /locus_tag="ACFOF2_000902"
     CDS             9252..9980
                     /gene="rpe"
                     /locus_tag="ACFOF2_000902"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC7107247.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(10003..11034)
                     /gene="ruvB"
                     /locus_tag="ACFOF2_000903"
     CDS             complement(10003..11034)
                     /gene="ruvB"
                     /locus_tag="ACFOF2_000903"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC7107248.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(11116..11424)
                     /locus_tag="ACFOF2_000904"
     CDS             complement(11116..11424)
                     /locus_tag="ACFOF2_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107249.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            complement(11647..12900)
                     /locus_tag="ACFOF2_000905"
     CDS             complement(11647..12900)
                     /locus_tag="ACFOF2_000905"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC7107250.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
     gene            complement(12972..14600)
                     /locus_tag="ACFOF2_000906"
     CDS             complement(12972..14600)
                     /locus_tag="ACFOF2_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC7107251.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            14750..15124
                     /locus_tag="ACFOF2_000907"
     CDS             14750..15124
                     /locus_tag="ACFOF2_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC7107252.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            15184..16722
                     /locus_tag="ACFOF2_000908"
     CDS             15184..16722
                     /locus_tag="ACFOF2_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC7107253.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKVKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPDDAAARIPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            16789..17406
                     /gene="recR"
                     /locus_tag="ACFOF2_000909"
     CDS             16789..17406
                     /gene="recR"
                     /locus_tag="ACFOF2_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC7107254.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(17462..17740)
                     /locus_tag="ACFOF2_000910"
     CDS             complement(17462..17740)
                     /locus_tag="ACFOF2_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107255.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            17945..19192
                     /locus_tag="ACFOF2_000911"
     CDS             17945..19192
                     /locus_tag="ACFOF2_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC7107256.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDIGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            19185..19880
                     /gene="lolD"
                     /locus_tag="ACFOF2_000912"
     CDS             19185..19880
                     /gene="lolD"
                     /locus_tag="ACFOF2_000912"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC7107257.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNHHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            19948..21693
                     /gene="recD"
                     /locus_tag="ACFOF2_000913"
     CDS             19948..21693
                     /gene="recD"
                     /locus_tag="ACFOF2_000913"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC7107258.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(21765..>22265)
                     /locus_tag="ACFOF2_000914"
     CDS             complement(21765..>22265)
                     /locus_tag="ACFOF2_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC7107259.1"
                     /translation="LAAILAKQGIPSVIGTDTNPKAVACARANIARLGFEKQVEIRET
                     DLFPEGFADLIVCNPPWLPAKPTSAVESALYDPESAMLAAFLRDAPKHLNPDGEIRLI
                     ISDLAEHLHLRPSDFLDKAFAQAGLRVADVLKTKPVHKKAADPNNPLAFARNRETTFL
                     YRLKKA"
BASE COUNT         5144 a         5607 c         6292 g         5222 t
ORIGIN      
        1 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
       61 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
      121 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
      181 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
      241 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
      301 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
      361 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
      421 tctgtctgta ccgccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
      481 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
      541 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccgt ttgcctcttc aaagccgtgc
      601 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
      661 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
      721 agccaaggca agagccaccc attccgcctg cgctgcctca tccgcacact ctgcgcattc
      781 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagattg gaatggaaat
      841 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctcc cccaaatcca accaacggcg
      901 tacatccgag gcaacattca tccagccgct ttgcgcgcaa cggcaggcaa gtgaaacatc
      961 ggcaacattt tcatctagat tccatatgtt ttccaaacct tctccctgtt gggacaggca
     1021 tatcagttgg aaagcctggt tttcagacgg catcaaggca tagaccgccg cactccgcac
     1081 gcctgtcgag cgcgaaaaca ccgaatccaa agccatgaaa agccgtttca gtgcatcttc
     1141 gcggacattg tcgcacgaca ggtaatcggc aagtttccaa ccctcatcac tgcgccacaa
     1201 aaccgaacgg tctatcgaag ccgtccccat acccattacc gtctgcgccg tcagatacgc
     1261 cgcccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     1321 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     1381 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     1441 accttgcaca aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga cgtggcgaaa
     1501 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     1561 gtccgcacca ataatgataa ataaattata tagttaatgc ttatcgggac aaattagtgc
     1621 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     1681 acacgcagaa cggtacataa agtgccgcca tttgttccgc accgaatcgg aaacgggtgc
     1741 ttccgcccgc aatcagattc accgccccta atgccggggt ttcaaccgga aagaagaaaa
     1801 tattgatttt gatgatggca tcaaaaaacc ccttccgctc gtctgccaaa aggaatggtt
     1861 aagaaaagca atgcgtcttt tgatggaacg cgatatataa ggaattgagg ggaagggcag
     1921 gcgggtttct gcccgttaat cctgcggacg gtcccgccaa acccgacacg cgcgggtata
     1981 tggcggactg tttacaatat tgtctaaaaa tctgaacctg caaacgtgaa ataaggtaga
     2041 atacgccccg ttattttacc gtccaatccc catttaccaa ggaaaaacga tgagtacttc
     2101 attgagttac cgcgatgcag gtgtcgatat cgacgcaggc gaccaactgg tcgaaaacat
     2161 caaaccgttt gccaaacgca ccatgcgtcc ggaagtattg ggggatttgg gcggttttgg
     2221 cgcattggtc gaaatcggca agaaatataa aaatcccgta ttggtcagcg gtacggacgg
     2281 cgtgggtacc aagctcaaac ttgccttcga ttgggataaa cacgatacgg tgggcatcga
     2341 ccttgttgca atgagtgtca acgatatttt ggttcaagga gcagaacctc tgtttttctt
     2401 ggattatttc gcctgcggca aattggatgt tccgcgcgca accgatgtca tcaaaggcat
     2461 tgcacaaggt tgcgaagaat ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc
     2521 gggtatgtat cccgtcggag aatacgattt ggcgggtttt gccgtcggcg tagtggaaaa
     2581 agagaatgtc attaccggcc gcagcatcgg cgcgggcgat gtggtattgg gtttggcttc
     2641 caacggcgca cattcaaacg gctattccct tatccgtaaa atcatcgaac gcgacaatcc
     2701 cgatttggat gccgagtttg ataatggcaa aaccttgcgt gaggctgtta ttgcgccgac
     2761 ccgtctgtat gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta aaggtatggc
     2821 acacattacc ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgcctg aaaacacggt
     2881 tgcacaaatc gatgctaaat cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc
     2941 gggcaatgtg gaaacccaag aaatgtaccg aacctttaac tgcggcatcg gcatggtcgt
     3001 tattgttgct gccgaagatg ccgatgcggt tcagggtctc ttgggtgaac aaggtgaaac
     3061 agtttaccgt ttaggtttga tccgtgagcg tcagggagac gagcatcaaa cccaggttgc
     3121 ctgattgctt ctatagcgaa atgccgtctc aaacatgggt ttggacggca tttttatagt
     3181 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacggcaag
     3241 gcgaggcaac gccgtactgg tttttgttaa tccactatat attgattgat atgagggtgg
     3301 gttacacatg cttaaacgat ataagccatt gtatatttat gggattcgga atggaacatt
     3361 ttaccctgca ttagtatttc cgctttttat tatgccgtct gaaaatatca tgatgatgcg
     3421 ttttcagacg gcatagctga tattgcagta tcagataggg gcaaacggaa aacactgaac
     3481 cgaacaatat tactgctatg ccaagcgtac ttatgggggc ataaacttac atgcttggtc
     3541 tgttatccga atgaccgtac ggggattaaa acccctaata tgccgtctga acccgcctta
     3601 ttccagcatg ggttggggga cgttgtgccg gatggcgtaa acagcggcct gcacccggct
     3661 gctgaggttg agtttgcgga gcaggttttg aacgtggact ttgacggtgg attcggcaag
     3721 atcgaggtgg cgggcgatga ttttgttgct gtgccctgcg gcgagatagc ccaagatttc
     3781 cagttcgcga ggggtaagtg aggagagtgc ctgtgtccct tgggcaggtt ggggggaaat
     3841 caggctttta acgagtttgg cggtcatctc gggcgagaat acattatcgc cttcggcggc
     3901 tttgcgtatg ctttcgagca gaaagtcagc gttgatgttt ttcagcaggt aaccgcgcgc
     3961 gccgatgcga atacattcgg tcaagtcgtc gctgtcttcg gaaacggtca gcataatcac
     4021 tgcctgctgc ggattgatgc tgatgatttg ggagagtgct tcgcgtccgt tcatgccggg
     4081 catatccaaa tcgagcagga cgacatcggg ttgcagccga ctaatcattt tgatgcccga
     4141 gaggccgtct gcggcttcgc cgatgacttc aaaaccgtgt tggcgcgaca aaagggcttt
     4201 aatgccgctg cggaagaggg tgtggtcgtc tatcagaata attttaatgg tcatttcaag
     4261 ctttcttcag atgcacccgt caatgagacg gtggttccct gttggggttg ggaacggatt
     4321 tctaaaacgg catggatgcg cttggcacgc tcctgcatga tgtgcagtcc gacatggctg
     4381 cccgtaggtt ctcctatttt ctccgtgtcg aaaccttgtc cgttgtcttg gatggtcatg
     4441 gtaaagcgtc cgccgtgttc ggaaagggtg aattttacat gggtggcgcg ggcgtgtttg
     4501 cggatgttgg acaggctttc ctgcaggata aaaatcattt ggagctgcgc ttcctgaggc
     4561 ggcaggaacg aaccgttttc ccaggcggtt tcgaccgtta tcccggtttg ttgcgtaaag
     4621 cgggcgaata ggtcggcaac ggcttcggga aattctttat tgctgatttt ggtacggaag
     4681 ttgagcagca gttcgcggac atcttcataa cattcctgca cgcctgtttt gatgaagccg
     4741 atgttttctg cggcttcctc ccgtttgttt tcggcaaagg cggtttccag catctgtacc
     4801 tgtaggttta ggaacgttaa tgcttgtgcg atgctgtcat gtaatccttg cgcaatcagg
     4861 ttgcgttcct gcaatactgc aagcaggcgt ttttcttcct cctgttttgc gccggcaagc
     4921 gatacgccca attgcctgcc tagtgtttga agcaggatgc ggtcgtcttc atcaagagaa
     4981 atgccgtttg gaaagctgag caacagcctg cccaatgttt cgttctggta ctcaatgggg
     5041 aagatttcct catggtactt ccccaaatcc gaagctgctg tgccgcaatc cgcatgatga
     5101 atggaaacat aaacatcgga tccgccgtcc aaacaaactc tgccggaatc tgctcctacg
     5161 gcgggcagga tacggtttag aaaatgttct gcagcctgtt gcggtatgta ggattggtgc
     5221 aggtcccgtg tagtttggta cagcagggtc aggttttgat tttgtttttc gagactgcgt
     5281 gtctgctcgg cgacttgtcc ttccaaatca tcatataaaa ttttcaacct gccgcccatt
     5341 tgattgaaac aacgcccgac ctgtttgaat tccggcgtac cgccttcggg aaccggaata
     5401 tcgaaacacc tccgtccgat gcgttccgca ccttccctta acgcctgcag cggccggata
     5461 acccaaatct ggtgccaaaa cagcatcagt acagacgaca ccagcgtcat caacataatt
     5521 gcccattgaa aacgcctgag ccaccatgtg tttttttcgt tggcattttc caatgcctgc
     5581 aaaaacagtt cgatgtttcc ggcaaagcgg tagagatcga cctgagtcgg tcgccggtag
     5641 gactggagcg gggggaggat gtgtgcctgc caatctataa tcagcatgga ttgtatcaaa
     5701 tcataagcaa gaggggtgtc cgaaggaatc agcggatgga tggcatcgct ttgggcaatg
     5761 cgttttaaac ttttttcaaa ttcggcaacc tgattgtcaa tttgcgcacg gggcgagcct
     5821 tcacccgcca tgtatgccag acggtatgcc tgcattctca agttgcccgc ctcttcgatg
     5881 acggaggccg cgttttccag acgcaaagag agcagcagtg tcaaaacgac agacaatgcc
     5941 gccaacccga cccacagtcc ggtcaggagt ttcaggcgca gggaaaggct gatgccgtct
     6001 gaaaaacggg ctggcagtat catgctcggc gaaaaattgt tctaaataat gcaaacaata
     6061 tcattctttg gaattagata caactgctca gaaagaattg gttaaggaaa acttaatccg
     6121 caccgcttca gtgttaaatc atgttatgat aaaattcaga taatatgaat ttatttgccg
     6181 tctgcttcag acggcatttt gagtcattgg gaacggcaga ctgcaatcat gaaaacctcg
     6241 aacttttcca agagaacatc cgcaatgaaa acttttgccc tgatactggc cggcggtcag
     6301 gcgagccgta tgggaggcga ggacaagggg cttgctcttt tggggggtaa ggcactgata
     6361 gaccatgtca tcgacagggt caggccgcag gtcagccata tcgccatcag caccaaccgg
     6421 aatttggaag aatatgctcg aagaagtccg catatttttc ccgatgcgcg gcagtggcag
     6481 catttcggcc cgctttcggc attgtgtacc gcagccaacg atttgcagtt ggcgactgct
     6541 gactggcttt tggttgtgcc gtgcgatatg ccgtatctgc cggacgattt ggtggcgagg
     6601 tttgaaaccg tgtcgaaacg cacaccgttg tgcaatgcgt attatgtgga aacgccgata
     6661 acgatgcact acaacattat gtatatccgc ccgcagattc tgcaaagcgc gattccctat
     6721 ctgttttcgg gtatgaaaac attaagaagc tggttgcagc agcaaagggc gcggtcggtc
     6781 agattcgagt ttgacgggca ttttgccgac ttgaacacgc aaaccgattt gcaggaggga
     6841 taagggcaag accgccgacc ggcgtggaaa ggaaaggtca agccataccg ggcggttttt
     6901 gcccgaatcg gaggcataat gctgtctgaa ggcatttcag acggcatttt tcgcggggag
     6961 atgctacaat ttgcaccatt tttaccgaca cagggaaaca ggattatgtt tacaggcatt
     7021 gttcaaggat tgggaaaact gacggcaatc caccgcccgt cggaggcatt tcagacttat
     7081 gtcgtcgagc ttccgcaaga ggcggcggac aatctgcaac gcggcgcatc ggttgccaat
     7141 aacggctgct gcctgacgat taccgaaatc gaaggaaacc gcgtcagctt cgatttaatg
     7201 gcggaaactt tggcaaaaag caatttgggg ctgctgaagg aaggcgattg cgtcaacatc
     7261 gaacgggcgg cgcgtttcgg agacgaaatc ggcggacacg tcatgagcgg acacattatg
     7321 gcaaccgtgc ctattgtcga aatcgaacgg gacgggttca accgcacggt ttggttttcg
     7381 ctcccgcatg aactcaaacc ctatatcctg accaaagggt tcgtcggctt ggacggttgc
     7441 agcctgacca taggcaaagt cgaagacggc cgtttcaatg tccatctgat tcccgaaact
     7501 ttggaacgga cgctgttcgg cagcagaaag gtcggcgaca ggatcaacat cgaaatcgat
     7561 ccgaatacgc aggcaatcgt cgacaccgtc gaacggctga tggcgcaaag atatgcaaag
     7621 tgaggtagag atggaattga acgaatttct cgataaagcc tatgccgttt tgcggcgttt
     7681 ggatgccgtg cttccgcccg aacccgggca tacggattgg aacgcgcttg ccttccgctg
     7741 gcagagtgcg ggcaaaaaag gttttttgga acacttgccc gatccgcaca cctttccctt
     7801 ggtaaggctg gcgggagtcg gcaggcaaac cgaattgctg gtgcgtaata ccgaacagtt
     7861 cattgtcggc agacccgcga ataacgtatt gatgagcggc gcgcgcggaa caggcaaatc
     7921 ctcgctggtc aaagccctgc tacacgaata tgcgaataag ggattgcgcc tgatcgaagt
     7981 cgataaaagc gatttaatcg gcctgcctta cctgttgacg cttttgaagg aacgtccgga
     8041 aaaatttatc gtattttgcg acgatttgtc gtttgaaagc ggcgatgaaa cctataaggc
     8101 attgaaaacc gcgttagacg gcggtctgtc tcaacgttgc gccaacgttt tggtttacgc
     8161 gacttccaac agacggcatc tgatgcccga atattttgac gagaatgccg gtacgacggg
     8221 gatgcggggg gaaatccatc agaaagaagc ggtggaagaa aaaatatccc tgtccgaccg
     8281 attcggattg tggctcagtt tttatccgtt cgatcaaaac gattatctgg cggcggtgca
     8341 aaattggctg gaagatttcg gcgtgccgta tgatgaaacc gcacagatgg cggcattgca
     8401 gtgggcgcag acgcggggca gccgttcggg acgttccgcg tggcagtttg cctgcgactg
     8461 ggcgggcagg ctgccgaaac aacgggcgtt gtgagcgttt cggggattat ttgaggcagg
     8521 aagaaagcca catttgctgc tgttgcggtg tgaggatatg gaagaaccgg tgttggattt
     8581 ccaattcgtc caccgcaaaa tccataccgg acaaatagcg gctttcgaca taatcgcgcg
     8641 cctcgttccg attaaaaaca tccgaggaaa tgatttcgac gacagaccgg cggcggctgt
     8701 gttcggaatg cataaccttc aaacgcgccc tgtcgcccgc cattttgaag gcggcgcgga
     8761 ttttacgcag ttcattgtgc tgaccctgtg tcagtccgag ccggcgtatg tcgcagttcg
     8821 gttgaaaatc gttcaggggg atataagccg tggcggcgtg ggaaagctgg caggaaagca
     8881 aagccatact taaaaaagag gcggcaggtt tggcaaaacg gcagggagca ggcagtggca
     8941 cggcttttcc tcttgttgga aattttagga aatatactag attgacgtgt gaaagatggt
     9001 attatcgcgg ttaacttttg ttaataatgc gtacgtatgt ccggcaggca gacataacgg
     9061 cggaatcgga acgtatagtg gattaacaaa aaccagtaca gcgttgcctc gccttagctc
     9121 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatctgta
     9181 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa aggatattga
     9241 aggaaaaaaa catgactact taccgtatcg cacccagcat tttatcggca gattttgcac
     9301 ggcttggcga agaggtggaa agcgtcattg cggcaggtgc agatctgatc cattttgacg
     9361 tgatggacaa ccattatgtg ccgaacctga ccttcgggcc tatggtttgc gcggcgttga
     9421 agccttatgc aagcgtgccg atcgatgtgc atctgatggt cgaacccgtg gatgacctga
     9481 ttcagtcgtt tgccaaagca ggagcatcaa tcatcacgtt ccatcccgaa gcgagccgcc
     9541 atattgaccg cagcttgagc ctgattcggg acatgggctg tcaggcgggg ctggtgttga
     9601 atccggcaac gcccgtatat ctgttggaaa acgtattgga caggctggat atggttttgc
     9661 tgatgtcggt aaaccccggg ttcggcggtc agagcttcat tccgtatact ttggaaaaaa
     9721 tcagacaggt tcgtgccatg cttgaccgct atgaagagaa aagcggcaga cgtattgcca
     9781 ttgaagtgga cggcggcatc aaaaccgaca atatcgccgc cgccgcccaa gccggcgcgg
     9841 atacctttgt tgccggttct gcgattttcg gcaaaccaga ctataaggcg gtgattgatg
     9901 cgatgagggc ggaactggaa aaaagcggca ggctgaacct tgcgccaatg attgaaatag
     9961 aaatgccgtc tgaaaattag ctctgtttca gacggcattg cgttattttt cgacgggcag
    10021 cccgaaatgc aggtaggcgc gttcggtcgc catcctgccg cgcggggtgc gttgcaggaa
    10081 gccttgttgg ataaggtagg gttcgataac gtcttcgatg gtgtctgtag attcgccgat
    10141 ggcggcggca acattgtcca aaccgaccgg gccgccgccg aatttgtgca aaacggcttc
    10201 gagaaatttc ctgtccatca cgtccagccc ctgcgcgtcc acgtccagca tacttaaagc
    10261 ggcatcggcg atgccgccgt cgattgtgcc gttgtttttc acgtcggcga aatcgcgcac
    10321 gcgtcgcaac aggcggttgg cgatgcgcgg cgtaccgcgg ctgcgtttgg cgatttcttc
    10381 cgcgccttct tcggacatat cgagctgcaa cagttgtgcc gaacggctga cgatggtggt
    10441 aaggtctcgg ttttcgtaaa actcaaggcg ggagacgatg ccgaagcggt cgcgcaacgg
    10501 attggtcagc ataccggcgc gggtggtcgc gccgatgagc gtgaagggcg gcaggtcgat
    10561 tttgacggaa cgggcggcgg gtccttcgcc tatcataatg tcgagccggt agtcttcgag
    10621 cgcgggatag aggatttctt cgacaacagg gctgaggcgg tggatttcgt cgatgaacaa
    10681 tacatcgtgc ggatcaaggt tggtcaaaag ggcggcgagg tcgcctgcgc gttcgaggac
    10741 ggggccgctg gtttggcgca aatttacgcc caattctttg gcgatgatgt gcgccagtgt
    10801 ggttttgccc agtccgggcg ggccgaagag caaaacgtgg tcgagtgctt cgccgcgttt
    10861 tttggcggct tggatgaaaa tggcaagctg ttctttggct ttgtcttgcc cgatgtagtc
    10921 gtccagcgtt ttggggcgga gggcgcgttc gagcagttct tcctgtgcgg aggcggtttg
    10981 ggcggcaacg atgcgttgcg gttgcgcggc ggtcaggttg tcggtttgca gcatagtgtt
    11041 ccctttgtcg ggtatgccgt ccgaacggtc ggcggcgttt cagacggcat tgaaaagata
    11101 acggtcaaaa gcgttttatc cgcgggtttc gcgccaaacg agatagtcgc gcaggggcgg
    11161 cataggcggg ttgatgcagt gggggcagat gccggtggtg tcttcgtcgt cgctgtcgat
    11221 gtggtaggcg ttggggtcga agcggatttg ctgcatgacg gcttgcgcca atgcctgcgc
    11281 ctgaacaacg tcgaaattga atttttcgag gatttcgttc aacaggcgga tttcgccacc
    11341 gctaaattcc gcactgtgtt tgaggatgag gcgttgataa tcttcgttgt tgattttagg
    11401 caataaatcg gttttcaggg tcatgatttt atgtcggggg caggatggct gaagcgtgaa
    11461 ttatagcgga tatggcggcg gctgttgcaa agcttggatt gggtatcgtc ctcaaaactt
    11521 tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact atctgtactg
    11581 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaaagt ggctgcattg
    11641 gacgttttaa tgtttttctg ttttgtcttg cgccgctcga aacgtttttt gcaatggggc
    11701 gatttgtgcc agccgcgccc ggtcgatttg ttcggcgata aggtgcgctt ttccctgcgc
    11761 gcggcattcg gcggcgattt tgcccgaatc cgcctgattg gcggcttcga gcagggcgaa
    11821 ccagtgtgcg cgttgcgggt agggcgtgtg ttcgcggttc agacggcctt gcgtgtcggc
    11881 aatgcagacg ttcaatgccg tctgaaagcg ttcgggacgg cggaaggcat cggttttttt
    11941 caatacgttc agaatggttt ggcttttaag ctgtccgact tggtggaaaa taatgtgcca
    12001 acggcaaacc aattcggcaa gctcggcgca atgtttcggc gcacgcagcc gctgattgac
    12061 ttcgcgcacg ggttcgacac cggcgaggtc gtgtccgtgg tggcgcggca ggatgtcgga
    12121 cggtgttttg gctttgccca agtcgtgcag cagggcggca tagcgttcgg gcaggctcaa
    12181 acccatatcg gcggcgcgtt gcagcgtcat cagggtatgg atgccgctgt cgatttcggg
    12241 atggtagtcg gcgcgttgcg gcacgccgaa gagggcattg acttcgggca gcaagacttt
    12301 gagcgcgccg cattcgcgca acacttcaat cattttgcgc ggattttttt ccatcaaacc
    12361 tttcgcaaac tcctgccaga cgcgttcggc aaccaatgcg tccgcttcgc cgttttccac
    12421 catctgccgc atcagcttta tggtttcttc ggcgatttca aacctgtaac gcgcggcaaa
    12481 gcgggcagta cgcaggatgc ggacggggtc ttcggcgaag gccggggaaa cgtggcgcaa
    12541 aatgcctgcc gccaaatccc gttgtccgcc gaaagggtcg ataatcttgc cgtccgcatc
    12601 ttgcgccatc gcgttgatgg tcaggtcgcg gcgcatcaag tcctgctcca gcgtaacgtc
    12661 tttgtcggcg tggaaactga aaccgacgta acctttggcg gttttgcgct cggtgcgggc
    12721 gagggcgtat tcttcgtgcg tttcgggatg gagaaacacg ggaaaatctt tgccgaccgg
    12781 ctggaagcct tgcgccagca tggtttgtgc gtctgcgccg acgaccaccc aatcgcggtc
    12841 tttgacgggc aagcccaaaa gataatcgcg gacggcgccg ccgacgaggt aagtctgcat
    12901 atgcttccta ttttaaagtt atcaacaatg ccgtctgaag cggcttcaga cggcattgtt
    12961 ccaaccggcg gttatgctac gccttttttc tccaagtaac tttcgtaatc gcccaagtag
    13021 tgttcatatc cgcctttgcc gtccagttcg atgatttggg ttgccaagga ggaaacgaac
    13081 tgacggtcgt gcgagacgaa aatcagcgtg ccgttgtatt tttccagcgc catgttcaag
    13141 gattcgatac tttccatatc catgtggttg gtcggttcgt ccatgaccaa gacattgggt
    13201 ttcaacagca acagtttgcc gtaaagcata cggccttttt ctccgccgga gagaaccttc
    13261 acttttttca cgacatcgtt actgccgaag agcaaacgcc ccaaagtgcc tcggatgact
    13321 tgttcgtcgt cgccttcctg cccccattgg cgcatccatt cgctcagatc catatcgacg
    13381 tcgaagtcgt tttcatggtc ttgcggatag tagccgacgc tggctttttc cgcccatttg
    13441 atgctgcctt cgtccggcag caggccgtct gaatattcgg ggttgtacgc gcccgccaag
    13501 agtttcagca gggtagattt gcccgcgccg ttcggaccga tgatggcgag acgctgaccc
    13561 gcttcaagga tgaagttcag gtttttaaac aactgggttt caaagcgttt cgccagtttt
    13621 tccacttcca cagcctgacg gtgcaacttg gctttttcat cggcttcaaa acggatatac
    13681 gggttttgac gggtagaagg tttgacttcg accatctccg atttgatttt gtcggcctgt
    13741 ttcagacggc tggttgcctg acgggctttg gatttgttgg cagagaagcg ggcgacgaac
    13801 tcttgcagct cttgcagttt ctctttggct ttggcattgt ctttcagggc gcgttcgcgc
    13861 gattgggcgg aggcgagcat gtagtcgtcg tagttgcccg gatagatggt gatggtgttg
    13921 tagtccaaat ccgccatgtg agtgcagact tcgttcaaaa agtggcggtc gtggctgatg
    13981 ataatcatcg tggagtcgta ttggttcaac acgccttcca accagcggat ggtattaatg
    14041 tccaagttat tggtcggttc gtccaagagc aatacatccg gcttggagaa cagcgcctgc
    14101 gccagcaata cgcgcagttt gaagcccggg gcgacttccg ccattttcgc attgtgcaaa
    14161 tcttcggaaa tgcccacgcc gctcaacagt tcggcggcac gcgcttcggc ggtgtagccg
    14221 tcgtattcgg cgaacttggc ttccagttcg gcggctttca tgtagtcgtc ttcggtggct
    14281 tcgggattgg cgtaaatcgc atcacgttcg gtcatcgccg cccacatttc ggtatgcccc
    14341 atcatcacca cgtccagcac gcgcatgtct tcgtaggcaa actggtcttg gcgcaattta
    14401 cccaaacgca cgccgttttc aatcgccact tcgccggccg tctgttccaa atcgccgccg
    14461 aggattttca tgaaggtgga tttgcctgag ccgttcgcgc cgatcaaacc gtagcggttg
    14521 ccttcgccga atttaacgga tacgttttca aacagcggct tcgcgccgaa ctgcatggtg
    14581 atgccgttgg tagaaatcat gattggtaaa gcctttattg aacaagagat taaatttttc
    14641 gaattttcgg attgtaacat aaccgccctg ccgtctgaaa cagacgggca aattttcaga
    14701 cacttatagt aaaatatccg ttttattatt tcagacaaaa ggtttccaaa tgatagtcct
    14761 tcacggcatc ccaaattgcg atacggtcaa aaaagccaaa aaccggcttg ccggatacgg
    14821 cttggagttt gaatttcggg attttaaaaa acaggtgcct tccgaagcgg aaatctgctc
    14881 gtggctggaa caagtgcctt tggcaaccct gctcaacaaa cgcgggacaa gctggcgcaa
    14941 actcgatgcc gaaacacagc aaaaagccct gttctcgaca gcggaggccg tcaaactgat
    15001 gtccgaaatg ccgagcctga tcaagcgtcc cgtattggag tgcggcggca aggtttacgc
    15061 cggcttcagc gaagaaacct acgacggcat cttcaaccgg caagccccgt gcagacaggg
    15121 ataaaaaccg ttacaatacc cgacttgaat ttcccgttcc cattctatat cccgatttaa
    15181 aatatgttcc attccatcga aaaatacaga acgcccgccc aagtcctttt gggcctgatt
    15241 gcattaacct tcgtcggctt cggggtcagc acggtatccc atccgggtgc cgactacatc
    15301 gtccaagtgg gcgacgaaaa aatcagcgac cactccatca acaacgccat acagaacgaa
    15361 caggcggacg gcggcggccc ttcgcgcgac gcggtgttcc aatccctgct gcaacgcgcc
    15421 tacctgaaac agggcgcgaa gctgatgggc atttcggttt cttccgaaca aatcaagcag
    15481 attatcgtgg atgatcccaa tttccacgac gcaaacggca aattcgacca cgcgctttta
    15541 aaccgctacc tgtcgcaacg ccatatgtct gaagaccagt ttgtcgaaga aatccgcgat
    15601 cagtttgcct tgcagaattt ggtaaacctc gtccaaaacg gcgtattggt cggcgacgcg
    15661 caggcggaac agctgatcag gctgacgcag gtcaaccgca ccatccgttc gcacactttc
    15721 aaccccgacg aattcatcgc ccaagtcaaa gtgtctgaag ccgatttgca gaagttttat
    15781 aacgcaaaca aaaaagacta cctgcttccc aaagcggtca aattggaata tgtcgccttg
    15841 aatctgaaag actttgcaga caaacagacc gtcagcgaaa cagaagtgaa aaatgcgttt
    15901 gaagagcgcg tggcgcgttt gccggcaaat gaagccaaac cttctttcga gcaggaaaaa
    15961 gccgccgtag aaaacgaatt gaaaatgaaa aaggcggttg ccgacttcaa taaggcaaaa
    16021 gaaaagctgg gcgatgacgc gttcaaccat ccttcctcgc ttgccgaagc cgccaaaaac
    16081 agcggtttga aagtcgaaac ccaagaaact tggctgagca ggcaggatgc gcaaatgtcc
    16141 ggtatgcccg aaaacctgat caatgccgta ttcagcgacg acgtattgaa gaaaaaacac
    16201 aattccgaag tgctgaccat caacagcgaa accgcgtggg tcgtccgcgc caaagaagtc
    16261 cgcgaagaga aaaccctgcc gtttgccgaa gccaaagacg cggtacgtca ggcttatatc
    16321 cgtaccgaag ccgccaaact tgccgaaaac aaggcaaaag acgtgcttac ccaactgaac
    16381 ggcggcaagg cagttgacgt gaaatggtcg gaagtgtccg ttttgggcgc acagcaggca
    16441 aggcagtcca tgccgcccga ggcttatgcg gaactgctga aagtaaaacc ggcaaacggc
    16501 aaacccgcct atgtcaggct gaccggcctg ccggcacccg tgattgtcga agtacaggct
    16561 gtaaccccgc cggatgatgc cgccgcgcgc atcccgcttg caaaacaggc tttggcgcag
    16621 cagcagtctg ccaatacctt cgacttgttg atacgttatt tcaacggcaa aatcaaacag
    16681 accaaaggag cgcaatcggt cgacaacggc gacggtcagt aattgacact tttgttgaca
    16741 acataacggt cggaatattc cggccgtttt cccatacggc ggaatatgat gagccacaaa
    16801 aaacaagatg ccttccaagg attgatcgac gcgctgaagg ttttacccaa cgtcgggccg
    16861 aaatcggcac agcggatagc gtatcatttg ctccaacaca agcgcaaaga ggctgaaaaa
    16921 ctggtggatg ccttgcagac ggcattgaag caggtttacc attgcgcgat gtgcaacacg
    16981 ttttgcgaag gcggattgtg cgatatttgt gccgatgaaa cacgcgacgg gcggcggctg
    17041 atggtggtgc atatgcctgc cgacgtgtcg aatatggaag cggcaaactg ccacgacggg
    17101 ctgtatttcg tcctgatggg gcaaatcaat acggcattgg gaatggacgt atccgccatc
    17161 gcattggaca ggctggcgca acggctgggc ggcggggaag tcgaagaaat cattattgca
    17221 accgctttta ccgcagaagg caatgcgacg gcgtatgtcc tgtccgagtt ttttaaaaac
    17281 ctgccttaca aagtcagcag gctgtcgcag ggcattccct tgggcggcga attggaatat
    17341 gtcgatgcgg gaacgctggc gcaggcggtg tacgaacgcc gcctgattaa agaaggcggg
    17401 gcataacgcc gccaatgcaa aatgccgtct gaagccttca gacggcattt ttccgggcgg
    17461 tttacttgca gtttagccct tgaatctgca atgcgcctcc gttgccgact ttgaacacgc
    17521 actgcgtcgc gctgctgccg gacacggtaa tgctgtcgct gccgccgttc aggctgatag
    17581 gttccttaac acccataccg actgccgttg ccgccagctt ttgcgcgtcg gcttcggaat
    17641 aaggcgtgtt gttggcatcg attttgattt ctttggcgta ggcagccgca gcggcggata
    17701 aggcgatggc gcacaaagca aaagcagttc ggatattcat tgttttcctc aagaaacggt
    17761 ttttctgaag gaaaggatag ggcagtttga cgcgttgtgc aagttttccg cccgctctgc
    17821 cgccccgtcc cccgcactcg ggcgttcgga cgctgccggg gttttacggt acaataaact
    17881 tttgccgccg cccccaaacc gccgtccggg gcggcacgtt tgcagacatt tttaaggtag
    17941 cgttatgttt tctctagagg cttggatagg cttgaggtat ctcagggcga aaaagcgcaa
    18001 cggctttatg tcgtttatca cgatggtttc gattgccgga atcgccttgg gcgtaaccgc
    18061 gctgattgtc gtcttgtcgg ttatgaacgg ctttcagaaa gaaatacgcg ggcagctcct
    18121 gaatgtcgcg ccgcacgccg aaatcggcta tatcgacaat acggatacgg attggcgcaa
    18181 cctgcttcgg tttaccgaaa accgcaaagg tattttggct gccgcgccct atgtttccaa
    18241 tcaggcattg ctggccaatg cgggcgaaat caggggcgtg cagatgcgcg gcattttgcc
    18301 gtctgaagag cgcaaagtgg tggaatacgg cgacaaaatg ccggcaggca aatttgaaga
    18361 cctgattccg ggcgagtttg acattatcct cggtgtcggc ttggcggagg ctttgggggc
    18421 ggaagtcggc aataaagtta ccgtcatcac gccggagggc aacgttaccc ctgccggagt
    18481 cgtgccgcgc ctgaagcagt ttaccgtggt cggtctggtt aagacgggcg tttacgaagt
    18541 ggacaactca ttggcgatga cgcatatcca agacgcgcgc gtactgtacc gcttggataa
    18601 ggaagttgcg gggctgcggc tgaaactcgc cgacccgcaa aacgcccccg ccttgacggc
    18661 aacactgatt ccggaggcgc aaagggatac ggtttgggtg cgcgattgga cgttcagcaa
    18721 tcgaagctat tttgaggcgg tcgagttgga aaaacggatg atgttcatca tcctgacgct
    18781 gattatcgcc gtggcggcgt tcaaccttgt ctcttccttg gtgatggcgg ttacggaaaa
    18841 gcaggcggac attgcgattt tgcggacttt gggtctttcc cctggcggcg tgatgaagat
    18901 ttttatggtg cagggcgcgt tttcaggctt tttcggcacg ttggcgggtg tggtctgcgg
    18961 cgtgcttttg ggttggaacg tcggcagggt cgtggcgttt tttgaaaacc tgctcggtgt
    19021 ccacctcatc aattcgcagg tttattttat cgactacctg cccagcgatg tcgatatagg
    19081 cgacgttgcc ctgattgcct gcatttcctt ggggctgtct ttcgtggcga cgctctaccc
    19141 gagccggcgc gcgtcaaaaa cccaaccggc ggaggctttg cgttatgagt gagttgattt
    19201 tgaaatgcga aggcgtgggc aaacgctatc gggacggcgg tttggacgtt ctggtgctgc
    19261 acggcttgga tttggaaatc cgcgcggggg aaagcaccgg catcatcggt tcttcgggca
    19321 gcggcaaatc gacgctgctg catattttgg gcgggctgga tatgccgtct gaaggcaggg
    19381 tgctgctgat gggcgaggat ttacgtacat taagccagcg acaactgggc atcctgcgca
    19441 accaccatct cggtttcgtg taccagttcc accatcttct gcctgaattt tcggcactgg
    19501 aaaacgtaat gatgccgctt ctgatcggca aaaaaagccg tgaagaggct gcggaggcgg
    19561 caatggcgat gctggaaaag gtcggactga aacaccgttc gacgcaccgc gcgggcgaac
    19621 tttcaggcgg tgagcggcag cgtgccgcca ttgcacgcgc cttggttacc caacccaaat
    19681 gcctgcttgc cgacgaaccg accggcaatc tcgatcgtgc gaacgccagg aatgttttgg
    19741 atatgatgct ggaactgaaa acagaattgg gaacggggct ggtggtcgta acgcacgacg
    19801 acgaactcgc cggccgcttc gagcgcgtga tggtcatgaa agacggcagc ctgcacccca
    19861 aacagggcgc aaacgcctaa acagccgaaa caacaccgcg ccgtctgaag ccccacgcct
    19921 tcagacggca tttggataag gaataaaatg gaacggcaga cagatgaatt tgcccaagcg
    19981 gcggcaaggg cggcaatccg ttttttggaa cactatgcgg gttccggcga cgaagtgctt
    20041 gcaaactgca ccgaacgcct gtttcaggca ttgcaaaacg gtcattcgtt tatccgtttg
    20101 agcggtggcg aggctgacgc gctgtcggca ctcgcacccg ttgtcggaac atccgccgcg
    20161 cctttgattt tggaaggcag aaggctgttt ttgggcagga tgtggcagtt ggaatacgat
    20221 ttggctgccg agataaaacg cttggcggca gccgacacgt ccgcccccga cgcggcaggc
    20281 gcgaggcaaa acctcgcaaa atggtttcaa ggcacgggca gcgaagggca gcgcgatgcc
    20341 gccgccttgg cactgttgca gtcttttatg gtgattaccg gcgggccggg aacgggcaaa
    20401 acgacaacgg ttgccaaact gctggcgctg atttgcggtg aagacgaaaa tcttcccaac
    20461 atcgcgcttg ccgcaccgac gggcaaagcg gcggcacata tggcgcgcgc gctgcaccgt
    20521 gcaatcaacg gttttgacgc gccggaggcc gtccgccgcc atttgctcaa actggaaggg
    20581 cagaccgtcc accgactgct gaagctgcgc ccgcccaaaa tgcaggcggc gttcaaccct
    20641 gtttacccgc tgccgtttga cgtattggtt atcgatgaag cctctatgct ggatacggca
    20701 ttgatgctgc aacttttaaa agcggtcaaa accggcgcgc gcgtgattct gctgggggat
    20761 gaaaaccagc tcccgtccgt cggaataggg gcggtgctgt ccgttttgtc acaaaaaacc
    20821 gttttggacg gagaaacgca ccaaaggctg gccggcttcc ttccggaaca cggtttcagc
    20881 gtcagcgcaa atccacccgt gttggcgcaa aacaccgccc atctgtcgtt cagccaccgc
    20941 ttcggcgaca acagcggcat cggctgcctt gcccgtgccg ccgtatcggg cgatgaaggg
    21001 gcgtgggcat tgtttgaccg gtttccggac gaactggaac attcggaatg cagtccgaac
    21061 gctcgagtcg aaaggttgta ccgggcacac aaagcctatt ggcaggcggt aaaagacggc
    21121 aatatcgaag ccgcatacgc gggcatttcg gatatcgtgg ttctggcagc ttggcggcag
    21181 gatgcggaag acttcaacga agcctattgc cgccatgtac gccgcaaaat gaacataccg
    21241 gaacatttgg catattttgc cggagagccg attatgatca ggcagaacga ctacgcgctt
    21301 gaactgttca acggcgacat cggactgatt atggaagatg tcggacggca gggcagcctt
    21361 gccgcctatt ttgccgatgc ggacggattt aaaaaggtag cggtaagctg cctgcccgaa
    21421 tttgaacccg cattcgccat gaccgtccac aaaagccaag gttcggaata ccgggaagta
    21481 tggctgctgc cgccttccgc cgcaccttcg gacgaagggg acgatgcatt gtccggattg
    21541 agtaaggagc tgttatatac cgccattacc cgcgcgagag agaagttcgt attcttcggc
    21601 ggggaagaag ccttccggca agctgccgcc accgtcaaaa cgcgtcagac ggcattgggc
    21661 agtatgctcg agcgggtatt ttcacaagaa taatccgccc gaatgatgcc gtgccggcgg
    21721 cgcgggcgtt ccgatgcctg ccgccggaag cgcaccgccg ccccttatgc ctttttcaaa
    21781 cggtacagga aagtagtttc cctgtttcgg gcaaaagcca gcggattgtt cggatcggca
    21841 gcttttttgt gaaccggttt ggttttcaaa acatccgcaa cgcgcaaacc cgcctgagca
    21901 aatgccttat ccagaaaatc ggacggacgc aggtgcagat gttcggcaag atcggaaatg
    21961 atcaggcgga tttctccgtc gggattcaga tgtttcggcg catcccgcaa aaacgcagcc
    22021 agcatcgcag attcggggtc gtataacgcg gattcgacgg cggaagtcgg cttggcggga
    22081 agccagggtg gattgcagac aatcagatcg gcaaaccctt cgggaaacag atcggtttcc
    22141 cgtatctcaa cctgtttttc aaagcccaaa cgggcaatat tggcacgggc gcaggcgacg
    22201 gctttcggat tggtatccgt gccgatgacg gaaggaatgc cctgtttcgc caaaatggcg
    22261 gcaag
//
LOCUS       DAXIHS010000027        21620 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXIHS010000027 DAXIHS010000000
VERSION     DAXIHS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.027"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..239
                     /locus_tag="ACFOF2_000915"
     CDS             <1..239
                     /locus_tag="ACFOF2_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107260.1"
                     /translation="GHTFPKPPPDFLIWRPAHHFRPIETPVLKDCVYFHRRFKGRRYS
                     ADGAQTAFRRHFHSFGVSVRIALPVRAISPCGLP"
     gene            complement(314..1777)
                     /gene="guaB"
                     /locus_tag="ACFOF2_000916"
     CDS             complement(314..1777)
                     /gene="guaB"
                     /locus_tag="ACFOF2_000916"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC7107261.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(1918..4476)
                     /gene="glnD"
                     /locus_tag="ACFOF2_000917"
     CDS             complement(1918..4476)
                     /gene="glnD"
                     /locus_tag="ACFOF2_000917"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC7107262.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSNHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(4525..4845)
                     /locus_tag="ACFOF2_000918"
     CDS             complement(4525..4845)
                     /locus_tag="ACFOF2_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC7107263.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(5169..5642)
                     /gene="bfr"
                     /locus_tag="ACFOF2_000919"
     CDS             complement(5169..5642)
                     /gene="bfr"
                     /locus_tag="ACFOF2_000919"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC7107264.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(5670..6134)
                     /gene="bfr"
                     /locus_tag="ACFOF2_000920"
     CDS             complement(5670..6134)
                     /gene="bfr"
                     /locus_tag="ACFOF2_000920"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC7107265.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(6598..7581)
                     /gene="lipA"
                     /locus_tag="ACFOF2_000921"
     CDS             complement(6598..7581)
                     /gene="lipA"
                     /locus_tag="ACFOF2_000921"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC7107266.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(7574..8197)
                     /gene="lipB"
                     /locus_tag="ACFOF2_000922"
     CDS             complement(7574..8197)
                     /gene="lipB"
                     /locus_tag="ACFOF2_000922"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC7107267.1"
                     /translation="MKVIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(8199..8474)
                     /locus_tag="ACFOF2_000923"
     CDS             complement(8199..8474)
                     /locus_tag="ACFOF2_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC7107268.1"
                     /translation="MTEQKNKTSLIEFPCTFSLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQIKVENQEQLDNIYRALTSHELVKVVL"
     gene            8659..9729
                     /locus_tag="ACFOF2_000924"
     CDS             8659..9729
                     /locus_tag="ACFOF2_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC7107269.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLAFALGDTLAPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNTLKAWFPVLMRQGSNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(9926..10873)
                     /locus_tag="ACFOF2_000925"
     CDS             complement(9926..10873)
                     /locus_tag="ACFOF2_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC7107270.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(10888..11295)
                     /locus_tag="ACFOF2_000926"
     CDS             complement(10888..11295)
                     /locus_tag="ACFOF2_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC7107271.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            11376..12035
                     /gene="ung"
                     /locus_tag="ACFOF2_000927"
     CDS             11376..12035
                     /gene="ung"
                     /locus_tag="ACFOF2_000927"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC7107272.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(12142..12498)
                     /locus_tag="ACFOF2_000928"
     CDS             complement(12142..12498)
                     /locus_tag="ACFOF2_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107273.1"
                     /translation="MMKKQRHILCVGCLYIILKVNVFPNTGWMEASKGMQGISFSTFG
                     YADAPGYTFSRIVHTLIFLLGKTTGTRTTVSDSSHNVYYVKLLPKPSTKRLCRICRQA
                     NKSCFWKWRQKMPSEP"
     gene            complement(12543..13058)
                     /locus_tag="ACFOF2_000929"
     CDS             complement(12543..13058)
                     /locus_tag="ACFOF2_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107274.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(13524..15446)
                     /locus_tag="ACFOF2_000930"
     CDS             complement(13524..15446)
                     /locus_tag="ACFOF2_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC7107275.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKFTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(15523..15894)
                     /locus_tag="ACFOF2_000931"
     CDS             complement(15523..15894)
                     /locus_tag="ACFOF2_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC7107276.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            16091..17399
                     /locus_tag="ACFOF2_000932"
                     /pseudo
     CDS             16091..17399
                     /locus_tag="ACFOF2_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
     gene            17392..17826
                     /locus_tag="ACFOF2_000933"
     CDS             17392..17826
                     /locus_tag="ACFOF2_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107277.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(17885..18154)
                     /locus_tag="ACFOF2_000934"
     CDS             complement(17885..18154)
                     /locus_tag="ACFOF2_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC7107278.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(18336..20798)
                     /gene="lon"
                     /locus_tag="ACFOF2_000935"
     CDS             complement(18336..20798)
                     /gene="lon"
                     /locus_tag="ACFOF2_000935"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC7107279.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            21049..>21620
                     /locus_tag="ACFOF2_000936"
     CDS             21049..>21620
                     /locus_tag="ACFOF2_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC7107280.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQT"
BASE COUNT         4579 a         5295 c         6041 g         5705 t
ORIGIN      
        1 cggggcatac tttcccgaag ccgccgcccg attttttgat atggcggccg gcgcatcatt
       61 tcaggccaat cgaaacgccc gtattgaaag attgcgttta tttccaccgc cgttttaaag
      121 gccggcggta ttcggcagac ggggcgcaaa cggcgttcag acggcatttt cattctttcg
      181 gcgtgtccgt ccgaattgct ttgcccgtcc gcgcaatcag cccgtgcggc ttgccttgaa
      241 cggacaaaaa atgccgtctg aaacccgaaa atcaggtttc agacggcatt ttgccttgaa
      301 aaggctgttc aaatcagcgg tggtagttcg gtgcttcttt agtgatttga acgtcgtgaa
      361 cgtgcgattc gctcatacct gcggaagtga tttccacaaa ttcggctttt tcgtgcattt
      421 cggcaatatt ggcgcaaccc aagtaaccca tactggaacg cagaccgccg gtcagctggt
      481 ggatgatgtt cacaatcggg cctttgtaag gaacgcggcc ttcgatgcct tcggggacgt
      541 atttgtcggt gctgtcggtt ttgtcttgga agtagcggtc ggcagaacct tggctcatcg
      601 cgcccaatga acccataccg cgataggatt tgtacgagcg gccttgatag agttcgattt
      661 cgcccggcgc ttcttccgtg ccggcaaaca taccgccgag catgacgctg tacgcgcctg
      721 cggcgagggc tttggcgatg tcgccggaga agcggatgcc gccgtcggca atcaggggaa
      781 cgcctgtgcc tttgagggct tcggcaacgt tgtggatggc ggtcagttgc ggcacgccca
      841 cgcctgccac gatacgggtg gtgcaaatcg atcccgggcc gataccgact ttgacggcat
      901 ccgcaccggc ggcgaccaaa tccaaagcgg ctttggcagt ggcgatattg ccgccgatga
      961 cttggatgtg cggataggtt tctttcaccc atttcacgcg gtcaatcacg ccttggctgt
     1021 gcccgtgggc agtatcgacg acaatcacgt ccacgccggc ttcgaccaag gctttgacgc
     1081 gctcttcggt atcgccgccg gtgccgactg ccgcaccgac gcgcagacgg ccttcggagt
     1141 ctttgttggc gttgggaaac tcggtggttt ttaaaatatc tttaacggta atcagacctt
     1201 tgagttcgtc tttttcgttc agaaccaaaa cgcgctcgac tttgtgcgtg tgcatcagtt
     1261 cgcgcgcttc gtctatgctt gtgccttcgg ggacggtaac cagacgttcg cgcggggtca
     1321 taatggcgga aacgggcaaa tcgacgcggt tttcaaaacg caggtcgcgg ttggttacga
     1381 tgccgacgac tttgccgttt tcaacgacgg gcaggccgga cattttgcgt ttgcgctgcg
     1441 cacgcatttc caagacttcg cggatgagcg tcgtcggtgc aacggttacg gggtctttga
     1501 ccacgccgct ttcgtggcgt ttcactttgg aaacggcgcg cgcctgcatt tcgggcggca
     1561 tgtttttatg gatgatgccg atgccgcctt cctgtgccat cgaaatggcg aggcgcgcct
     1621 cggtaacagt gtccatcgca gcggaaagca gggggaggtt gagtgtgatt tcgcgggtga
     1681 gcttggtttg aagtttaacg tctcgcggca gcacggtcga atgtgcggga accaacaaaa
     1741 catcgtcgaa agtataggct ttttctacga tacgcataat gctcggtctt tcagtttgtg
     1801 caagatgcac ggcattatag cacgttaccg gcggcttgac agtttatatt gtttgcaata
     1861 tcccccgcct tcaggcggca tacggcgtgg cggcggaatg ccgtctgaag gcgggcgtta
     1921 tgataattgt tccagcagcg tctgcttcaa tgaggactgg atttttgggt ttttcaggtc
     1981 ggggctaaaa acggtaaaac tgtcttcggc gcgttcatcc agtgtggaga ttttggcata
     2041 gcgcaggctg acgttgtggg cgaaaaagat ttccgccata tcggcgagca ggaaggggcg
     2101 gttgacggcg gtaatttcga cggagtacca gtcggggtag tcttcttcgg gggtaatggt
     2161 gatgcttggc gcgatcggca tgtagcggct gcggcggctg atgcggcggc tgtggtttga
     2221 ggtttcggca acggtgtgtc cgtggataaa gctgttgagt tcggcttcaa gcgcgctttg
     2281 aatgtcgggg tagtcttcgg gggcgtgttg tgagggaatt tgcacgatga aggtgtcgag
     2341 gatgtagtcg tgttcggtga taaaggcgcg ggcggcgagg atatcgaagc cgtggcggct
     2401 gaaaatgcgg cagagacggg caaacaggcg cgggccgttg ggcatgaaaa ccatgacttg
     2461 aaagctgtcg cttttgggca ggatgcggct gcggacgatg ggggtttcaa agtcgtggac
     2521 taggttggcg gcgtgccaca ggatttcgcg ggactggtgg cgggcgaagt aggcggaacc
     2581 gagcgcgttc caaagctttt tctgctgttt ttcggggacg gcggcgcggg tgagcaagtc
     2641 ggcggcttct tgttggcggc ggccgaagag ggcgtgcggg ttgccaccgt tgcctgcgag
     2701 gtagcgtccg gcggcatgga agaggctttc cagcaggctg gcgcgccatg cgttccacag
     2761 cttgggattg gtaccgcgta tgtcggaaat ggtcagaagg tagagcgcgc tgaggcgttc
     2821 gtgggtttgg acgcgtttgc agaaggcatc gagtacgtcg gggtcttgga tgtcttcttt
     2881 ttgggcgacg atggacataa gaagatggtt ttcgaccagc caggcaagca ggtcgctttc
     2941 ttcttcggtc aggaagtggt cggcggcaaa ttggcgcgcg tctgagatgc cttgtatggc
     3001 atggtcgccg ccgcgtcctt tggcgatgtc atggaaaaag gcggcaaggt agaggatgtc
     3061 ttgtttttca aaggactgca tcagtgcaga ggcgtagggc agctcgtggc tgtgcatatc
     3121 caaggcaagg cggcggacgt tgcggacgac ggcgaggatg tggtcgtcca cgggatagat
     3181 gtggaacagg tcgtgctgga gcaggccgac gattttttcc cacgcgggca ggtagcgtcc
     3241 caatacgccg tagaggttga ggaagcgcag ggtttgggtc agtccgttgc cgttgcggaa
     3301 aaagccggcg aagcggtggc ggttttcaga attttggtag aagctgcggt tgattttgcg
     3361 cgtcgcgccc caccatgcgc gcagggtttg cggttcgagc gcggtaatgt cgttgcgctg
     3421 ctgcatgatt tcgacgattt tgaaaatgtg ttcgggccgt ctgaaaaaaa tatcggtgtg
     3481 ccgcgcggcg atttggttgt tgacttggat gtagtcgtcg tcaatccgca gggtaacgcg
     3541 caacggggtg gaggaaacgc ggctttgcag cataggcgtg aggatgccgc ccagttgttt
     3601 gacggtttta atcgcgcggt aaaacacgcg catcagttct tcgctttggc ggcggaggtt
     3661 caagccttca taacccatgc tttcggcgac ttgcggctgc aaatcgaaca gcaggcggtc
     3721 ttcggcgcgc ttggcgttta aatgcagatg gatgcggatg tgggcgaggc ggcggtagcc
     3781 gtgcgaaagc ataccggctt cggcacgcgt caaaatccgc tgtttgagca ggtcgggcag
     3841 gtcggtcgcc aagccttgcg ccttcgctat ccaaagcagg gtgtggatat cgcgcagacc
     3901 gcccggacag cttttgatat tcggctccaa tactgccccc gaaccttgcg atttggcgtg
     3961 gcggtgttcc atctccacca gttttgcctc gacaaacgcc gccacattgc gctgcgcgtt
     4021 cattttttcc gctagtttat ccgccgtttg gcggttgcca aacaaaaacc tagcctctaa
     4081 aaacgctgtg tcccccgtaa tatcattgcg cacgctttca catagttcat caacgctgcc
     4141 gctttttaca gacggcatca gtttgcagtc ccacagggtt tggacaaacc gggcaatctg
     4201 ttcctgaatg ccgtctgaaa gcggggcagg ggagacaacc gccaaatcca catccgaaca
     4261 gggatacagt tcgccgcgtc cgaagccgcc taccgccatc aggcataacg cgctgttttg
     4321 aaaatactct gcccacaatg ccgccagtaa ggtttcgagt gccgccgtat attccctgaa
     4381 aaataccgac acgcggttgg ctttcaaata atgcgcttcg gcggcatcgc gctgctgttt
     4441 gaaggtttcc agtgctgaag acaggtttgc aggcattttt attctttcga ttggcgggaa
     4501 aaagggaggc ggatggttcg gcggtcaaat accgctttca gacggcattt gtcgggtatc
     4561 ggcggaattg gtcatcagct tcaaccgttc ctgcggcggc agcagcaact gatacgccgc
     4621 cacgcccaaa gccgccgcca ttttttcgat attcgacagg gcgatgttcc agcgtttgcg
     4681 ctcgactgcc gacacataag tcctgtccaa accgcattgc cgcgccaatt cttcttgcga
     4741 ccaacccttg ttcacgcgga aaagccgcat attgtatgcc aataccgccc gcaaatcctg
     4801 ttcgtcaggc agttcggcag gcagagtcaa tttgttgccc atcatttgct tccagataaa
     4861 tggttaaagt taagcatttg ctgttacgga ttttacttca cataaaagcc aaaaaaaagg
     4921 ggtggcggca gccacaccca aacacacaca catcaagagg aaagagataa aatcagacag
     4981 attggaaaac agcaaacaag caccctgaat ttcatatcgg tattatcaaa caaaaaccct
     5041 tatcaggtat tgattcaaat caatttagat tttattcgca aaccgaaaaa gaaaaacggc
     5101 atatccgttt atacggatat gccgtctgat ggtgcggggg actgctgtat ttggcagtag
     5161 tttgtgtttt agtcctcttg cgccgctgtt tgcaggtagt tgggcagacc gattttgccg
     5221 ataagctctt gctgggtctc cagccagtct atatgttctt cgttggtgtc tttttgtttt
     5281 tccaacaaat cgcggctgac gtaatcctgt tgcgcttctg ctgtggcgat ggcggcaagc
     5341 agggcttcgt gtttttcctg ttctttggtc aaatcgcagg cgatgatttc ttcggtggac
     5401 tcgccaatca gaagcttgcc cagttcttgc aggttcggca gaccttcgag gaacagaatg
     5461 cgctcgatca aatcgtcggc agctttcatt tcaacgatgg actgtttgaa gaaatgttcg
     5521 cccagttctt caaagcccca gtttttcaaa atacgggcgt gaaggaaata ttggttgatg
     5581 gttaccagca gcaagcctaa gtttttgttc agctcgcgga taaccaaacg gtcgcctttc
     5641 atacggactc cttttattcc gaatacggat tacagttggc tttggtagta gttgccttcg
     5701 ccaatcagct cgatcaggcg cagctgctgt tccagccagt gggcgtggtc ttcttcggta
     5761 tctttcagtt gggcgaccat caaatcgcgg ctgacatagt cttgcgcctc ttcgcacagt
     5821 ttgatgcctt ttttcaaagc gtcgcgcact tcgtattcgg tttgcaggtc ggctttgagg
     5881 caggaaacca cgtccgtgcc gatattcagc tcggcgcgcg ccattttcgg cgtaccgccc
     5941 agcatcagga tgcgtcggat gaagtcttcg gcgtgtgtgg tttcttcttc catctcgtga
     6001 ttgagacgtt caaaaagttt ggtgtagccc cattcggagt agaggcggga gtggataaag
     6061 tattggtcgc gtgccgccag ctcgccagac agcaattcgt tcatataatc aacaacagct
     6121 tgattgcctt gcataatatc tctctttccg taacttggtt ttcggtatgt ctgaacagca
     6181 tctgtgtcgc tattcgggat gcgtgcattg tatgcaaaag ctgtcggcat gacaaatttt
     6241 ctatttaaaa tacaaacaat tatcaaaaga aatagggcgt attccccgat gccttccaaa
     6301 tcagtatgct gtttcttatc ggttttttgt gctgttaaaa ctaagaatcc atatcatcca
     6361 taaagaacat ttacacttgt taaccatagc gaaaagcaaa tactgtacgg ttttttatca
     6421 aaatttataa tgaatcgttc tcattaaccg acagattctt ttgagattat cggattttgg
     6481 gaataaatta tgccgtctga ggggctttca gacggcatag acaattaaag ctgattaaac
     6541 aacgcggcag acagcagcgt aggaaattat tttttacttc ggaacggtat tttgggttta
     6601 atgatgcccg caaccgccgt ggctttcgcg taaggcttcg gcagcctgtt cgtcggcgtg
     6661 gtagctggaa cgtaccatcg cgccgatggc ggcattgctg aagcccagtt cgtatgcttc
     6721 tttttcaaag attttgaact gctcgggcgt aacgtagcgc aggacgggca ggtgtccgtc
     6781 tgaaggctgg aggtactgtc cgatggtaat catttcgata ttgtgcgccc gcatatcgcg
     6841 cataatttca cgcacgtctt cgtctgtttc gcccaagccg accatgatgc cggatttggt
     6901 cgggatatgc ggcatcattt ctttataacg ttttaataag tctaaagaat gttgataatt
     6961 agcaccggga cgggcttttc tgtacaggct cggatgggtt tctaggttgt ggttcatcac
     7021 gtcgggcggg gtttcggcaa ggattttgag tgcgatgtcc aagcgtcctc ggaagtcggg
     7081 gacgaggatt tcgattttgg tgttcgggct ggtttcgcgg atggctttga tgcagtcggc
     7141 gaaatgctgt gcgccgccgt cgcgcaggtc gtcgcggtcg acggaggtga tgacgacgta
     7201 acgcaggttc atggctttga cggattcggc gaggtttctc ggttcgtcgg ggtcgagcat
     7261 attgggccga ccgtgtccca cgtcgcagaa cgggcagcgg cgggtgcaga tgtcgcccat
     7321 aatcatgaag gtcgccgtgc ctttgctgaa gcattcgccg atgttggggc aggaggcttc
     7381 ctcgcaaacg gtgtgcatct tttgttcgcg caaaatgtct ttgatttcaa agaatttgcg
     7441 cgatgggagt ttggcgcgta tccattcggg ctttttcagt ttttcctgaa gggggacgac
     7501 tttgatgggg atgcgcgcgg ttttgtccgc gcctctgagt ttgatgccgc gtttggggtc
     7561 gtcggttttg atttcactca ttgttgtctg ctttcggtgt gaattgtgtt tcaaggtgtg
     7621 cggtaagttt ggcggcgact tcgtccggcg tggggcaggg ttggacaaaa tccgcgattt
     7681 gcgtcatttc cataccggcg tagccgcagg ggttgatgtg ggtaaacggg cttaaatcca
     7741 tattgacgtt gagcgcaagc ccgtgataga cggagccgtt tttgatacgc agccccagtg
     7801 aggcgatttt gcgttctccg acataaacgc cggggcgttt ggggtctgcc gccgcttcga
     7861 tgccgtattc tgccaatgtg gcgatgatgc tgttttcaag cgcggaaacg atgtttctaa
     7921 cactggtttt gcgccgtttg aaatcaatca tcgtataaac gaccaattgc ccgggcccgt
     7981 gataggtaat ctgcccgccc cggtcgattt ggacgacggg aatgtcgtcg cgaatcagca
     8041 ggtgttcggg ttttcccgcc agtccttgtg tgaacacggg cgggtgttcg acgacccaca
     8101 gttcgtcttc ggtgtcggca ttccgtccgg cattaaaggt tttcatcgct tcaaaagtcg
     8161 gcagatattc gaccaaacct ttgtgtatga ctttcatctc aaagtaccac tttgaccagt
     8221 tcgtgcgaag tcagcgcgcg gtagatgttg tccaattgtt cttggttttc aacctttatc
     8281 tgaacggtgg cgccggtata gttgcctttg ctgctcggac gcgtggtgat gtggtgcgcc
     8341 tgcgtgtcgg gggcgtggag gcggacggtg tctaaaaccg cctgctcgaa ctcgggatgc
     8401 accgcgccca ttacttttaa tgagaaggtg cagggaaatt cgatgaggga tgttttgttt
     8461 ttttgttcgg tcatgatgtg ctgccttatc gtgtacggta tgccgtctga aggcgggttt
     8521 gcctttcaga cggcatcgga tgtgcgttat tttagcctaa accgcgataa caggctatcg
     8581 ggaaggcgga ggcttttttg acggcgcggc ggttctgcta tactggcgcg caatattatt
     8641 tttagaaggg tggtttttat gtatcggagg aaagggcggg gcatcaagcc gtggatgggt
     8701 gccggtgcgg cgtttgccgc cttggtttgg ctggctttcg cacttggcga cacactggct
     8761 ccgtttgcgg ttgcggcagt gttggcgtat gtgttggatc ctttggtcga atggttgcag
     8821 aaaaagggtt tgaaccgtgc atccgcttcg atgtctgtga tggtgttttc cttgattttg
     8881 ttgttggcat tattgttgat tattgtccct atgctggtcg ggcagttcaa caatttggca
     8941 tcgcgcctgc cccaattaat cggttttatg cagaacacgc tgctgccgtg gttgaaaaat
     9001 acaatcggcg gatatgtgga aatcgatcag gcatctatta ttgcgtggct tcaggcgcat
     9061 acgggtgagc tgagcaacac gcttaaggcg tggtttcccg ttttgatgag gcagggcagc
     9121 aatattgtca gcagtattgg caacctgctg ctgcttccct tgctgcttta ctatttcctg
     9181 ctggattggc agcggtggtc gtgcggcatt gccaaactgg ttccgaggcg ttttgccggt
     9241 gcttatacgc gcattacagg caatttgaac gaggtattgg gcgaattttt gcgcgggcag
     9301 cttctggtga tgctgattat gggtttggtt tacggcttgg ggttggtgct ggtcgggctg
     9361 gattcggggt ttgcaatcgg tatggttgcc ggtattttgg tttttgttcc ctatttgggc
     9421 gcgtttacag gactgctgct ggcaaccgtc gccgccttgc tccagttcgg ttcgtggaac
     9481 ggcatcctat cggtttgggc ggtttttgcc gtaggacagt ttctcgaaag ttttttcatt
     9541 acgccgaaaa tcgtgggaga ccgtatcggg ctgtcgccgt tttgggttat cttttccctg
     9601 atggcgttcg gacagctgat gggtttcgtc ggaatgttgg caggattgcc tttggccgcc
     9661 gtaaccttgg tcttgcttcg cgagggcgtg cagaaatatt ttgccggcag tttttaccgg
     9721 ggcaggtagg cggttccgaa acatatagtg gattaacaaa aatcaggaca aggcgacgaa
     9781 gccgcagaca gtacaaatag ggcaacgccg tactggtttt tgttaatcca ctatatttga
     9841 agcggaatac aaccttgccc gggtttaaat aaaaatgccg tctgaacccc gaaaactgga
     9901 cttcagacgg cattttcatc acggcttatt tggcggtttt gctgctgtcg ataattttca
     9961 taccggcaga aatcaggctg ccgatgtcgg caacattggc gggcataatc agcgtattgc
    10021 tttctttggc aagattgttg aacgcggcga cgtattgttc cgcaatcttc aggttgaccg
    10081 catccgcacc gccttgggtt tgaagggcgg cggcaatttg acggatggct tcggcattgg
    10141 cttcggcaac aaggcgcaag gattccgctt cacctttggc gcggttgatg cgggcgattt
    10201 tctcggcatt tgacgcattg accgcagcct gagcctcgcc ttcggattgt tggatttcgg
    10261 cttcgcgctg accgctggca aggttgattt gttcgatttt acggccttcg gattcggcga
    10321 tacgggcgcg tttttcgcgt tcggcggtaa tttgcgcctg cattgagcga aggatttctt
    10381 gaggcggcac caagtcttta atctcataac gcaaaacctt cacaccccaa gccccggccg
    10441 cctcgtccaa agccgcaaca acagtactgt tgatttcgtc gcgttcttca aacgttttgt
    10501 ccaactccat acgcccgata acggaacgca gcgtcgtttg ggcaagctgg gtaattgcca
    10561 tgatgtagtt gctcgaaccg tatgaggcga gtttggggtc ggttacttgg aaatagatga
    10621 tgccgtcaac agtcagctgc gtattgtcgc gcgtgatgca gacctggctg ggtacgtcta
    10681 aagggatttc tttcagcgaa tggcggtagg caacgcggtc gataaaggga atcaaaatat
    10741 tcaaaccggc cgtcagggcg cgatggaaac gcccgagcct ttcgacaacg tggacttcct
    10801 gttgtgggat gacaacaaag gatttgaaac cgaaaacggc aacggctgcc aacaagataa
    10861 tgaaaaattc cataattcct ccgagtgtta aggttttgcg atgataagaa ggttgccttc
    10921 cttgcggacg attagggcgc gcgttcctgg ttcaagctct tcttgccccg tattttgcgc
    10981 ctgccagtgc gtaccgcgat aaaaaacttc gtaacggttg ccgcctgtgt gtcggaggat
    11041 ttcgacatat tgtccggcat ccaaatcctg atatgaatcc gtttcaactt ttctaacggc
    11101 ggttttggcg tgtacgaacc aaatacccag cgcggaaagc agagcggcgg tcaagacggc
    11161 ggcaggcgtg ctgccggtca gcccgtaagc aatgcccgaa cccgccaaag ccgcgctgac
    11221 aaccaaaaga taaaccgttc ccgtcaataa ttcgatgatt aagacggcaa cagcggcaac
    11281 aaaccataca gtcatacatt tccccacaaa gcgcgtcgtt tgacaaaata acgcaatatc
    11341 agcagtatag ccgaatttga aaggataggg cagatatgga cacttggcac gatgcactcg
    11401 gcggcgaaaa acagcagccg tattttcagg aaattttaaa tgcagtcagg caggaacgtt
    11461 tgtcgggaca aatcatctat ccgccggcgg cggatgtgtt caacgcattc cgcctgacag
    11521 cgttcgaccg ggtcaaagcc gtcattctcg gacaagatcc gtatcacggg gcagggcagg
    11581 cgcacggttt ggcattttcc gtccggcagg gtatccgcat accgccgtct ttactcaata
    11641 tctacaaaga attggaaacc gacatcgaag gcttttccat acccgcgcac ggctgcctga
    11701 cagcgtgggc ggagcagggc gtattacttc tgaacacggt tttgacggtg cgtgcaggac
    11761 aggcgcattc gcacgccctt ttaggctggg aacgctttac cgataccgtc atcaggcagc
    11821 ttgcgacaca ccgcaagcat cttgtcttta tgctgtgggg tgggtatgca caacaaaaaa
    11881 ggaaactgat agacagtcaa aaccatttga tattgaccgc accgcatccg tctcctctgt
    11941 cggcatatcg cggttttttc ggctgccgcc atttttcaca ggcaaacagc tatttgagcc
    12001 ggcacggtat cgatccgata aactggaagc tgtgaatgcc atctgaatgc caagttgctt
    12061 aaaccccact gaaggctgct tttgaaaccg gaaacaaagt gattaaggaa aaccggccca
    12121 taatgcaaaa ccgtctgaat cctagggttc agacggcatt ttttgccgcc atttccaaaa
    12181 acaacttttg ttcgcctgcc gacaaatccg acaaagtcgt ttggttgagg gctttggcaa
    12241 taatttaaca taatatacat tatgcgaact atcggaaaca gttgtacgtg tccctgtggt
    12301 ctttccaagt aggaaaatta aagtatggac aatgcggctg aatgtatagc ccggagcatc
    12361 cgcgtatccg aaggtactga acgatatgcc ctgcatccct ttcgatgctt ccatccagcc
    12421 ggtattggga aatacgttga ccttcaatat aatatacagg cagccgacac acaggatatg
    12481 ccgctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
    12541 ctttacatac gtcccaattc tctttgcagt gcttggatat tttgctgcct gtccaataca
    12601 ttgctttgta atgcatttat ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct
    12661 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cgttgctcaa ttctgcttcg
    12721 agaatggagc gtctgctgtt gtttacaggt gcttgttgag gcgggggcgt attggatttt
    12781 gccggcttgg atactgtttt gaccggggct ttatatttga cagcctgtcc gccgtttgac
    12841 ggtgatggtg tcggttcgga tgtttggggc gggatatagc gttcgctgct gtagttgccg
    12901 attgggggca aatcggttga gaggcagctt ttggacggct tggtggtgta aacggtttct
    12961 ccgttgattg tgcaggtgta gattttggcc gcattcgcac ccaatgggct tgaaatcagg
    13021 gaaaagttga ttaggattaa ggggagtttt gatttcataa tgtgcttgat gctcggataa
    13081 ttatttgatt ttttggtatt ttttgtaatg ctttcttggc gttttacggg cttttctttt
    13141 cggttgtttg cttttgattt tctttcgggg ctttggcagc ctgcttgttc tttttcccgg
    13201 caggtttttt gtttttcttt ttactttcta tgtgtatttt ggcttcttaa ctgaattttt
    13261 taattttcag gcggtatccg cctccctgat ggctgctgat tttaggtaaa tccgcatcgg
    13321 cgcacgatcc tgctggggcc gatgtatata cgcttttgcc atttgagttg caatggtata
    13381 cggaggcttg cgccgccgcg ccggaaaggg acaggagacc caaggcggca aaaagtctga
    13441 tgtttatacc ggtaaaaggc ggtgataacg cccgaattat accgttattg gcaggcaaag
    13501 ataagcaccc tgtccgcgct tctttatcgc tcggcaaact gtttctcgat ttcagtttca
    13561 atccgttcca attcttcttg acaagccagc caaacctctt cgatttgggc aagttgtgtt
    13621 ttgacttttg ccagcaggga taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa
    13681 gcgtcttctt gtgctaaaaa tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt
    13741 tctttttcgg cacggtctat tttctgctgt atcggcttgc cgcgtcgggc tttttcctga
    13801 cggatttgcg cttcgatgcg cttggtgtct ttgcggcttt ggctttgtgc ggatgctgcg
    13861 ggtgcggcgg cggcgttttc ctgtgccaaa cgccattggc ggtaatcgtt caaatcgccg
    13921 tcgaagttct tcagacggcc tttgtcgatc aggaggaagc tgtcggtagt cgcttcaagc
    13981 aggctgcggt cgtgcgatac ggcgattaat gcgccttgga aactttgcaa cgcgagcgtt
    14041 aaggcgtggc gcatatccaa gtccaaatgg ttggtcggtt cgtcaagcag caacaggttc
    14101 ggcttttgcc agataatcat ggcaagggca aggcgggctt tttctccgcc ggaaaaaggt
    14161 tcggttttct gtaacgccat atcgccaaca aaattgaagc cgccgaggaa atttcggatt
    14221 tcttgttcgc gtacttcggg agaaagctgc tgaatatgcc aaacagggtt ttggtcggcg
    14281 cggatggtgt cgagttggtg ttgggcaaaa tagccgatat tgagtttttc ggaacggatg
    14341 atgctgccgg aaagcaaatc gattttgcct gccaaggctt tgataaaggt ggatttgccg
    14401 ctgccgttga cacccaatag cccgtagcgt gcgccgcttt ccagcgacag ggtaatgtcg
    14461 tgtaggacag ttttgccttc gtaacccaaa tcagcgtgtt ctagctttaa caaaggattg
    14521 ggcagatggt cgggatggta aaactcaaag gaaaactcgc tgtccagatg cgcgggcgca
    14581 acgcgttcta gctttgccaa agccttcatg cggctttgcg cttgaacggc tttggtggct
    14641 ttggctttga agcggtcgat aaaggattgc aaatgtttga tttgcgcctg ctgtttgaca
    14701 taggcagctt gttgttgcgc gagacgctgc gcgcgttcgt tttggtaaaa atcgtaattg
    14761 ccgccgtatt gcgtgaattt ttgctgcgat aattcaatgg tttgggtagt tgccgcgttg
    14821 agaaaatcgc ggtcgtggga aatgatgatt tgcgtgcagg gtaaagaagc aagatggttt
    14881 tccagccaca agacggtttc caaatccaag tggttggtcg gttcgtccaa aagcagcaaa
    14941 tcggcgcggc aaatcagggc ttgggcgaga ttgaggcgca tacgccagcc gccggaaaag
    15001 gatttgacgg ggcggctgtg ttcttcttgc gaaaaaccca gcccgttcaa caattttgcc
    15061 gcgcgggccg gcgcggtata agcgtcgatt tcttccaatt tggcatgata ttccgcctgc
    15121 ttcatgccgt cattttgcgc ttctgcctgc ctcaatgccg tctgaaaagc ctgcaactcg
    15181 gcatcgccct gcaaaacgta atccaaagcg gaaatatcca aatcgggcgt ttcttgggaa
    15241 acggaagcga gccgccagtt tttcggaatc gagacatcgc cgccgtcctg agtgatttca
    15301 cccttgatta aggcaaacag gctcgatttg cccgttccgt ttttgccgat caaaccgacg
    15361 cgctgaccgg gattgacggt agcgttggct ttgtcgagca ggactttcaa accgcgttgc
    15421 agggtgaggt ttttgatttc aatcataacg gaaacatcgt cgggcgggaa aagcccgtat
    15481 tttacctgaa agtcagtgcc gatgccgtct gaaacgggaa atttacggct gaagccaagc
    15541 ccaagccctg cgcccttccg agtgcaggaa aaccaatgtc ctgaatgccg aatcggtatt
    15601 catgcattcc acgctgattc cgattcggga aaaatccgcc atgattttgg gatggataaa
    15661 ctcctgagcc gcgcccgtcc cgataatcaa tatttccgga tagtcaacag gtttgatgtc
    15721 ggacaacagg ttttccggag tcagatcgga caaggttcgg cattgcgaca ggcagaccga
    15781 atccttatgt acaagcacgg gtttatggaa actttgcccc gccagccgga ttccgcccgc
    15841 accgcattca tattccgcaa actgtccgtc tatcggattt tcttcaaaca acattttttt
    15901 accccgtcgc cgcatcatct acaccgaaag gggtgcaaaa tcagacaaat tcatgtagga
    15961 ttggcagatt tcatctgact cgcctgccga tttcagacgg catttgattc aaagtgcggc
    16021 accattatat cggcagcgga tattttcgtc tttcaatatt tacatttcag tcggcttaca
    16081 aggagacaca atgaagccag taaacatcgg tcttttaggt ttgggtacgg tcggcggcgg
    16141 tacggctgcc gtgttgcggg acaacgcgga ggaaatttcc cgtcgcttgg ggcgcgaaat
    16201 ccgtatttct gccgtatgcg acttgagcga agaaaaagcc cgccaaatct gcccgtccgc
    16261 agcctttgtc aaagatccgt tcgaactggt cgcgcgtaaa gacgtcgatg tcgtcgtcga
    16321 attgttcggc ggcacgggca ttgccaaaga tgcggtgttg aaagccattg aaaacggcaa
    16381 acacatcgtt accgccaaca aaaaactgct cgccgaatac ggcaacgaaa tcttcccgct
    16441 ggcggaagaa aaaaatgtca tggtgcaatt tgaagcggca gtagcgggcg gtatcccaat
    16501 catcaaagcc ctgcgcgaag gtttggcggc aaacaggatt aaatccatcg ccggcattat
    16561 taacggcacc agcaacttca tcctctccga aatgcgtgaa aaaggcagcg cgtttgccga
    16621 tgtactgaaa gaagcgcagg cactgggcta tgccgaagcc gacccgacct tcgaacatcg
    16681 aaggacatga cgcgggccat aaaatcacca tcatgagcgc gctggcattc ggcacgccga
    16741 tgaacttttc cgcctgctac ctcgaaggca tcagcaaact cgacagccgc gacatcaaat
    16801 acgccgaaga acttggctac cgcatcaaac tgttgggcat cacccgcaaa accggcaaag
    16861 gcatcgagct gcgcgtccac cctaccctga ttcccgaaag ccgcctcttg gcaaacgtca
    16921 acggcgtgat gaacgccgtg cgcgtcaacg ccgatatggt tggcgaaacc ttatattacg
    16981 gcgcgggcgc gggcgcattg ccgaccgctt ccgccgtggt tgccgatatc atcgacatcg
    17041 cccgcctggt tgaagccgat accgcccacc gcgtaccgca tctggcgttc caacccgcgc
    17101 aagtccaagc gcaaaccatc ctgcctatgg acgaaattac cagcagctac tacctgcgcg
    17161 tccaagccaa agacgaaccg ggcacgctgg ggcaaatcgc cgcgctgttg gcacaagaaa
    17221 acgtgtccat cgaagcactg attcaaaaag gcgtgattga tcagaccact gccgaaatcg
    17281 tgattctgac ccacagcacg gtcgaaaaac acatcaagtc ggcaatcgca gccatcgaag
    17341 cactggattg cgtggaaaaa ccgattacca tgatccgcat ggaaagcctg catgactgag
    17401 ccgaaacacg aaatgccgac ggaagagcag gttgccgcgc gcaaaaaagc aaaagccaaa
    17461 atccgcacca tccgcatttg ggcatgggtc attttggcgt tgctcgcttc aaccgccctg
    17521 ctctcccaat gcgcgatgtc caaaccgcag gcaaaacaga aaattgtcga gtcttgcgtg
    17581 aagaatattc cgtttgccga aaaatggcaa aacgatttac gggcccgcgg tttagattca
    17641 aacaataccc gccttaccgt cgactactgc aaatgtatgt gggagcagcc tttggacaga
    17701 ttgagcgaga aacagattag atccttcggc aaactcggcg cacaagaaca gcttgacctg
    17761 ctcggcggcg caaatgcctt tgaagcacgt gacaagcagt gtgttgccga tttgaaatca
    17821 gaataatgtg gaccgataaa aaagccgatt ctttaaagaa tcggcttttt tcataaaaaa
    17881 cggcttacag tgcgtctttc aaagctttgc cggcgcggaa tttaggcgtt ttggcggcgg
    17941 caatggtcag aggctcgccg gttttggggt tgcggccttg gcgttccgca cgttcgccca
    18001 cgtagaaagt accgaaaccg accaaagtaa cggtgtcgcc ttgtttcagg gcggtggtta
    18061 ctgcattggt agtggcatcc aaagcttttt gtgcggcggc tttggaaatg tcggcttctt
    18121 gagcaatcgc ttcgatcaat tcagacttgt tcacaatcag tcccttcctg tcttaaaaaa
    18181 tgatgaaatg cccgaatact cggggtttgt actgcttgag caactttcgc tttatagcaa
    18241 ttctgaaatt gccgtgtcaa gcaaaaaata cggaatcacc ctatttgaca ggctttcagg
    18301 acgaaaccgc atttttacaa cacatttcct gcgtttcaat gtttggttgc cctgctgcgg
    18361 ggttttggtt ttgaagcgga ttccgccgcc gcttccgcac cagaaggttc tgcccaaggc
    18421 tcaggctggc tttccaaacc cagagccaat acctcgtcta tccatttgac cggatggatg
    18481 gtcaggccgg ttttcacgtt ttcagggatt tcttccaagt ctttgaggtt gtcttttgga
    18541 attaaaacgt gtttgatgcc gccgcgaagc gcagccaaca gtttttcctt cagaccgcca
    18601 atcggcagga cttcgccgcg cagggtaatt tcgcccgtca tggcaacatc ggcgcgcacc
    18661 gggattttgg tgaatgcaga caccatcgcc aaagtcatgg caatgcccgc actcggaccg
    18721 tctttcggcg tcgcaccttc gggaacatgg acatggatgt cttttttctc gtaaaaatca
    18781 ggagccaagc ccactgattc cgcgcgggag cggacaaccg accacgctgc agacacggat
    18841 tccttcatca catcgcccaa ctggccggtg cactgaatca cgcccttacc cggcaatgct
    18901 gcggcttcga cggtcagcag ttcgccgccg acttctgtcc acgccagacc ggttacttgt
    18961 ccgatacggt tttcgctttc ggcaacgccg taatcgaagc ggcgcacacc caaatagtcg
    19021 tgcagatttt tctcatttac tttaaccgct ttaggtttgg ctttgctggt tttcttggtt
    19081 tcagacaacc tcttcttatc ttcgtccaag gtaatctgca tcaccacctt gcggcagatt
    19141 ttggcaatct cgcggtcgag cgaacgcacg cccgcctcgc gggtgtagta acggataata
    19201 tcgcgcaccg cgctttcttc gattgccaat tccccttctt tcacgccgtt gcgcttcatt
    19261 tgcttcggca cgaggtactg catcgcgata ttgatttttt cgtcttcggt gtagccggac
    19321 agacggatga tttccatacg gtcgagcagc ggggtcggga tattcaggct gtttgaagtc
    19381 gcaataaaca tcacatcgct caaatcgtaa tccacttcgg cataatgatc ggcgaacttg
    19441 ttgttttgct cgggatcaag cacttcgagc aacgcgctgg cgggatcgcc ccggaagtcg
    19501 ctacccaatt tgtcgatttc gtcgagcagg aacaatgggt tttttacgcc ggcttttgcc
    19561 atattctgca gaatcttacc aggcatagag ccgatatagg tgcggcggtg tccccggatt
    19621 tcgctttcgt cgcgcacgcc gcccaaagcc atgcggacat atttccgccc cgttgctttg
    19681 gcgatggatt cgcccaaaga ggttttgccc acgcccggag gaccgaccag gcacagaatc
    19741 gggcctttga gtttgtccat acgtttttgg acggcgaggt attccaaaat ccgttctttg
    19801 actttttcca ggccgtagtg gtcggcatcc agcaccagtc cggctttggc gatgtctttg
    19861 ctgacgcggg attttttctt ccacggcagt tcgagcaggg tgtcgatgta gttgcgtacg
    19921 acggtggatt cggcagacat cggcggcatc attttgagtt ttttcagttc ggacaggcat
    19981 ttttcttccg cttctttgct catccccgcc tttttgatgc ctgcttccaa ggcatccagt
    20041 tcgccgtttt cgtcttcttc gcccaattct ttgtgtatcg ctttaatctg ttcgttcaga
    20101 taatattcgc gctgggattt ttccatttgg cgtttgacgc gtccgcgtat gcgtttttcg
    20161 gcctgcataa tgtcgagttc ggattccagc tgtgccagca ggaattccat ccgtttgccg
    20221 atttcgggaa tttccaaaat ctgttggcgt tgcgccagtt tcaactgcaa atgcgctgcg
    20281 accgtatcgg ttagccggct gttttcggca atgccgttga tgctgccgat aatttcggcg
    20341 gggatttttt tattgagttt ggcgtattgt tcaaactgcg ccaacagggt gcggcgcacg
    20401 gcttcgaggt cggtattgcc gcccgtgtct tcttccacga ccgcctctat atgggaaacg
    20461 aacagaccgc ccgtgtcttc gatggtcaga acacgtccgc gatacagacc ttcgaccaat
    20521 acttttaccg tgccgtccgg cagtttcaat acctgtaaga cttgtgcgac ggtaccggtc
    20581 tgatacaggt cggcggcaac cggttcttct agcgccgcat cggtttgtgc caataggaaa
    20641 accggttcct cgcgggtaat ggcgttttcc agtgcggcga tggatttcgg cctgccgaca
    20701 aacagcggca gaaccatatg cgggtaaacg acgacatccc gcaaaggaag ggttgccaag
    20761 gcggcatatt cctcaaaatg cttttctttt tgtgtcatag gtactctctt gtgtctgaca
    20821 gattgccgat tttcgcgtac attggggttg aaggtattat ttcaagcata tgtggtttat
    20881 ttatggagtt tgatgcgatg ccgtctgaaa cattccggct tcagactgca tgggcttgga
    20941 aagacaaggc gggaacaaaa aactgttctg tgttgccgct ccttgctgta ccatccgtat
    21001 ggtttacggt tctgccgccc tattttcaaa acatgacgca ggtatcccat gtcttctttt
    21061 atttttcccg ataacggggc cgaatggcgc aacgaaagtg tgcaaaagcc gcccaaaggt
    21121 tggcattatg tccgcgaaag cggtgcagac ggcattttga aggctgccta tcaaaatatt
    21181 gcaactgtct ggcagggcga tttccacaat gccaaacagg tgctttctgc aatgaagaag
    21241 agggctcgca aacctgccgc cgtccgttcc gatgcggata tcgccgccct tttccatgcc
    21301 caccgtatga agcaggcgca gcagagccgt attctgaata tgcttgccgt tgaaatccgc
    21361 cccggttttg tgttggacaa caaacgcgcg cccgatatac gcgccgccct gctcgacgtg
    21421 tacggagagg cggacggcaa accgtttttc ctgccgctca atctgctgct ggggtttatg
    21481 ggtgcgcacg agtggcataa gaaaggggtt gccgttccgc agctgggcgg cagcatacac
    21541 gttcctttcg gcgtattctc gccgttgcgc ggcgaatacc tcgacctgct cgcccatgcg
    21601 ccgtcaacgg gttttcagac
//
LOCUS       DAXIHS010000028        21481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXIHS010000028 DAXIHS010000000
VERSION     DAXIHS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.028"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            286..1407
                     /gene="dnaJ"
                     /locus_tag="ACFOF2_000937"
     CDS             286..1407
                     /gene="dnaJ"
                     /locus_tag="ACFOF2_000937"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC7107281.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1603..3621
                     /locus_tag="ACFOF2_000938"
     CDS             1603..3621
                     /locus_tag="ACFOF2_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC7107282.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDTAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(3664..4221)
                     /gene="rfbC"
                     /locus_tag="ACFOF2_000939"
     CDS             complement(3664..4221)
                     /gene="rfbC"
                     /locus_tag="ACFOF2_000939"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC7107283.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLPLSR"
     gene            complement(4275..5141)
                     /gene="rfbA"
                     /locus_tag="ACFOF2_000940"
     CDS             complement(4275..5141)
                     /gene="rfbA"
                     /locus_tag="ACFOF2_000940"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC7107284.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            complement(5247..6287)
                     /gene="rfbB"
                     /locus_tag="ACFOF2_000941"
     CDS             complement(5247..6287)
                     /gene="rfbB"
                     /locus_tag="ACFOF2_000941"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC7107285.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            complement(6288..6908)
                     /gene="galE"
                     /locus_tag="ACFOF2_000942"
                     /pseudo
     CDS             complement(6288..6908)
                     /gene="galE"
                     /locus_tag="ACFOF2_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(6987..7331)
                     /locus_tag="ACFOF2_000943"
     CDS             complement(6987..7331)
                     /locus_tag="ACFOF2_000943"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC7107286.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDGAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(7324..7518)
                     /locus_tag="ACFOF2_000944"
     CDS             complement(7324..7518)
                     /locus_tag="ACFOF2_000944"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC7107287.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(7496..8326)
                     /locus_tag="ACFOF2_000945"
     CDS             complement(7496..8326)
                     /locus_tag="ACFOF2_000945"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC7107288.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(8357..10630)
                     /locus_tag="ACFOF2_000946"
     CDS             complement(8357..10630)
                     /locus_tag="ACFOF2_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC7107289.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALGRLKEAADTDAITVFARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(10695..11345)
                     /locus_tag="ACFOF2_000947"
     CDS             complement(10695..11345)
                     /locus_tag="ACFOF2_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC7107290.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(11342..12139)
                     /locus_tag="ACFOF2_000948"
     CDS             complement(11342..12139)
                     /locus_tag="ACFOF2_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC7107291.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(12139..13302)
                     /gene="ctrB"
                     /locus_tag="ACFOF2_000949"
     CDS             complement(12139..13302)
                     /gene="ctrB"
                     /locus_tag="ACFOF2_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC7107292.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(13317..14492)
                     /gene="ctrA"
                     /locus_tag="ACFOF2_000950"
     CDS             complement(13317..14492)
                     /gene="ctrA"
                     /locus_tag="ACFOF2_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC7107293.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            14627..15760
                     /gene="neuC"
                     /locus_tag="ACFOF2_000951"
     CDS             14627..15760
                     /gene="neuC"
                     /locus_tag="ACFOF2_000951"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC7107294.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPEMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            15764..16450
                     /gene="neuA"
                     /locus_tag="ACFOF2_000952"
     CDS             15764..16450
                     /gene="neuA"
                     /locus_tag="ACFOF2_000952"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC7107295.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNRKES"
     gene            16451..17500
                     /gene="siaC"
                     /locus_tag="ACFOF2_000953"
     CDS             16451..17500
                     /gene="siaC"
                     /locus_tag="ACFOF2_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC7107296.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            17652..20765
                     /locus_tag="ACFOF2_000954"
     CDS             17652..20765
                     /locus_tag="ACFOF2_000954"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7107297.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFGYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
     gene            20846..21481
                     /locus_tag="ACFOF2_000955"
     CDS             20846..21481
                     /locus_tag="ACFOF2_000955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC7107298.1"
                     /translation="MYSEQGINNTINISTTSLTNATQLTVIGNNNSVYIGNNCKIVSS
                     NIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKGKISIGKD
                     CMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVV
                     GSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNEFYK"
BASE COUNT         5859 a         4849 c         4877 g         5896 t
ORIGIN      
        1 gaaaaagacc tgcgctatat cggggaatga atcgacgcaa aggaaatgcc gtctgaacat
       61 gcgttcgggc ggcgttttta taacaaaaac acttcatggc ggttggtttt atgcctgtct
      121 aagtttttgt gtcgtgcata cctgaggatt tcagacggca tcggtttatg ctgtctgaaa
      181 agtgtattcc gtttcagttt gtaagctatg gcagtctgtt tgtcttgtgt tttgcgtaat
      241 tgcccttatt ttgagccgtg attttatttt gaattagatg aaaaaatgag taatcaagat
      301 ttttatgcga cgctgggtgt ggcaagaaca gctaccgatg atgagattaa aaaagcctac
      361 cggaaattgg cgatgaaata ccatcccgac cgcaatcctg acaataaaga ggcggaagag
      421 aagtttaaag aagtacaaaa ggcgtatgaa actttgtccg acaaggaaaa gcgcgctatg
      481 tacgaccagt atggtcatgc ggcgtttgaa ggcggcggac aggggggctt cggagggttt
      541 ggcggatttg gcggtgcgca gggttttgac tttggggata ttttcagcca aatgtttgga
      601 ggcggttcgg ggcgcgccca gcctgattat cagggtgagg acgttcaagt cggtatcgaa
      661 atcacgcttg aagaagccgc aaaaggtgtg aagaaacgca tcaatattcc gacttatgaa
      721 gcgtgtgatg tctgtaacgg cagtggcgcg aaaccgggga catccccgga aacctgcccg
      781 acttgcaaag gttcgggtac ggtgcacatc cagcaggcga ttttccgtat gcagcagact
      841 tgtccgacct gccacggtgc gggcaaacac attaaagaac cttgcgtcaa atgccgtggc
      901 gcggggcgga ataaggcggt caagacggtg gaagtcaata ttcccgccgg tatcgatgac
      961 gggcagcgta tccgtttgag cggcgaaggc gggccgggta tgcacggtgc gcctgccggc
     1021 gacttgtatg taaccgtccg cattcgggcg cataagattt tccaacgcga cggtctggac
     1081 ttgcattgcg aactgccgat cagttttgcc acggctgctt tgggcgggga gttggaagtg
     1141 ccgaccttgg acggaaaggt caagctcacc gtccccaaag aaacccaaac cggcaggagg
     1201 atgcgtgtga aaggtaaggg tgtcaaatcg ttgcgcagca gcgcgaccgg cgatttgtac
     1261 tgccatattg ttgtcgaaac gcctgtcaat ttgactgacc gtcaaaaaga gcttttggaa
     1321 gagtttgagc ggatttctac cggcttggaa aaccaaacac cgcgcaagaa atcgttttta
     1381 gacaagctgc gcgatttgtt tgattgattt taaggttcgg aaacaagcag ccgtatcggg
     1441 gaatctcctt gatacggctg tttttatttg tttaaaaata gtttttattt tcaatggggt
     1501 atgaggcagg gtgggataac tgtttttaac tgttcttttt aaaacttgac atcatggcgt
     1561 gatgccaaca atatgtgaac gtctgttgtc aaaggaagaa taatgaataa atctttatcc
     1621 agttcggtag aagaataccg cgagctgacg ctccgaggca tgatactcgg tgcattgatc
     1681 actgtaattt ttactgcgtc caatgtttac ctcggtttga aagtcgggct gacctttgcc
     1741 tcgtcgattc cggcggcggt gatttcgatg gcggttttaa agtttttcaa aggcagcaat
     1801 attttggaaa acaacatggt gcagacccaa gcttcggcgg cgggtacgct ttcgaccatc
     1861 atcttcgtcc tgcccggttt gctgatggcg ggctactgga gcggtttccc gttctggcag
     1921 acgacgcttt tatgtattgc cggcgggatt ttgggggtga ttttcaccat tccgctgcgt
     1981 tacgcgatgg tggtgaaaag cgatttgcct tatccggaag gtgtggcggc tgctgaaatt
     2041 ttgaaagtgg gcggtcatga agaaggggat aaccgtcagg gcggcagcgg catcaaagag
     2101 ctggcggccg gcggtgcgtt ggcgggattg atgagctttt gcgccggagg tctgcgcgtg
     2161 attgccgaca gcgcgagtta ttggtttaaa agcggtacgg cgattttcca gctgccgatg
     2221 ggcttttcac tggcattgtt gggcgcgggc tatttggtcg gactgacggg cggtatcgcc
     2281 atcctgttgg gcatttcgat tgcttggggc attgccgtgc cgtatttctc ctcacacatt
     2341 ccgcaacctt ccgatatgga aatggcggcg tttgcgatga agctgtggaa ggagaaagtg
     2401 cgttttatcg gtgcggggac tattggcatt gcggcggttt ggacgctgtt gatgctgctc
     2461 aagccgatgg tggaaggcat gaagatgtcg ttcaagagtt ttggcggcgg tgcgcccgct
     2521 gcggaacgcg ccgaacaaga tttgtcgcct aaagccatga ttttttgggt gctggcgatg
     2581 atgtttgttt taggcgtgtc gttttaccac tttatcggcg attcgcacat tacgggcggc
     2641 atggcttggc ttttggtggt cgtttgcacg cttttggctt ccgtcatcgg ctttttggtc
     2701 gccgccgcct gcggttatat ggcgggtttg gtcggctcgt cttccagccc gatttccggc
     2761 gtgggcatcg tgtccgtcgt cgttatttca ctggttttgc tgctggtagg cgaatccgga
     2821 ggtttgttgg cggatgaggc taaccgcaaa tttttgctgg cactgacttt gttttgcggc
     2881 tcggcagtaa tctgcgtggc ttcgatttcc aatgacaacc tgcaagactt gaaaaccggc
     2941 tacctgctca aagccacgcc ttggcggcag caagtcgccc tgattatcgg ctgtatcgtt
     3001 ggtgcgctgg ttatttcgcc cgtgttggaa ctgctttacg aagcctacgg ctttaccggc
     3061 gcaatgccgc gcgaaggcat ggatacggcg caggctttgg cagctcctca agcgactttg
     3121 atgacgacca tcgcgtcggg cattttcgcc cacaacctcg aatgggtcta catctttacc
     3181 ggcatcgcga tcggcgcggt attaatcgtc gtcgatttgg tgttgaaaaa atcatcaggc
     3241 ggcaaacttg ccctgcccgt ccttgcggtc ggtatgggta tttatctgcc gccgtccgtc
     3301 aatatgccca tcgtggcagg cgcggtgttg gcggcggtgt tgaaacacat catcggtaaa
     3361 aaagcggaaa accgcgaagg ccgtctgaaa aacgccgagc gtatcggaac cttgttctcc
     3421 gccggcctga ttgtcggtga aagcctgatc ggtgtgatta tggcgtttat tattgccttc
     3481 tccgtgacca acggcggctc ggatgcgccg cttgcgttga atctgcaaaa ctgggatgcc
     3541 gccgcttctt ggttgggttt ggcgttcttc gttaccggga tgtttttctt tgcacagcgc
     3601 gtactgaagg cgggcaagta ggctgtcgga aaaaatgccg tctgaagcgt tcagacggca
     3661 tttttatctg gaaagcggaa ggcggagctt ttcggcttgc gcccacgttt tgccggcaag
     3721 gtctttgggc gacagcagcg gcgcggtttg aagcggccag cctatgccga ctgtcgggtc
     3781 gttccatatt aaaacctgtt cggcttcagg cttgtaatag tcggtgcatt tgtacacgac
     3841 ttccgccgcg tcgcccaaaa cgcaaaaccc gtgcgcgaag ccttcgggta tccaaagctg
     3901 gcattggttt tgcgcggaca gggttgcgcc cgcccatttg ccgaaagtcg gcgaaccttc
     3961 gcgcatatcg acggccacgt cgaacacttc gccgacaact acgcgtacga gtttgccttg
     4021 tgtgttttcg gtttggtagt gcaggccgcg caatacgcct ttgccggatt tggagtggtt
     4081 ttcctgcacg aaggtgcgtt cgcagacttg ggttttaaac cactcgtcgc ggaaggtttc
     4141 cataaaaaag ccgcgcgcgt cgccgaagac ttggggctca agcagtttta cgtcaggaat
     4201 ggcggtatca atgatgttca tctttttatc tttcatctaa aggccgtctg aaaagtttca
     4261 gacggcctca aacattattt tttcaacagg cgcagcaaat attggccgta ttggtttttc
     4321 gccatcgggc gcgccaattc ttccagtttt tcatcggaaa gccaaccgtt gcgccaagcg
     4381 atttcttcca ggcaggcgat gtgcaggttt tggatgtttt gcacggtttg gacgaacgaa
     4441 gccgcctcgt gcaggctttc gtgcgtaccg gtgtccagcc acgcgaaacc gcgtcccaat
     4501 atttgaacgg agagcgagcc gtcttccaaa tacatccggt tgaggtcgga aatttccaat
     4561 tcgccgcgtg cggacggttt gagctgtttg gcgaactcga cggcgcggtt gtcgtagaaa
     4621 tacaagccgg ttaccgccca atcggatttg ggccgttgcg gtttttcttc gatggaaacg
     4681 gcgcggaagt tttcgttaaa ttcaaccacg ccgaaacgtt cggggttttt gacctgataa
     4741 gcaaacacgg ttgcgccgtg cgtttgcgct gccgcctgtt tcaatgtttg cgtaaacgac
     4801 tgaccgtaaa aaatattgtc gcccaaaacc aagcaaacat tgtcgttgcc gataaattct
     4861 tcgccgatga taaatgcctg tgccaagccg tccggactgg gttgcacggc ataactgatg
     4921 gaaatgccga aatcgctgcc gtcgccaagc aggcgtttga aagaggcgtt gtcttcaggc
     4981 gcggtaatca ccaaaatatc gcggattccc gccagcatca aaaccgacaa ggggtaataa
     5041 atcatcggtt tgtcgtacac gggcaggagc tgtttggata cgccgcgcgt gatggggtag
     5101 aggcgcgtgc cgctgccgcc tgccagtatg atgcctttca tcttttcttt cttcctttgc
     5161 gatgggtttt cagatggcat tgcgtcggga tgccgtctga aaacttattt tccggtgcct
     5221 aaacgttcca aacgatagct gccgttttat gcgttttgcc gccgagtttt gttgtccaag
     5281 taccattgca ctgttttgcg gaggccggat tcaaaggttt ccaaaggctg ccagcccaaa
     5341 tcccgtctga ttttcgccgt atcgacggcg tagcgggcat cgtggccagg gcggtcttgt
     5401 acgaaagtaa tcaaatcttc ataacgcgcc acgccggccg gtttttcggg agcgagttct
     5461 tccagcaggg cgcagatggt tttgacgact tcaatattgg ctttttcatt gtggccgccg
     5521 atattgtagg tttcgccgac aacaccttcg gtaacaacct gatacagtgc gcgcgcgtgg
     5581 tcttcgacaa acagccagtc gcggatttgc ataccgtcgc cgtacacagg cagcggtttg
     5641 ccgtcaagcg cgttcagaat catcaaagga atgagttttt ccggaaaatg gtaaggaccg
     5701 tagttgttgg agcagttggt tacaatggtc ggcaagccgt aagtacgcaa ccacgcgcgg
     5761 acgaggtggt cgctggacgc tttagaggca gagtaagggc tggacggcgc gtagggcgcg
     5821 gtttcggtaa acaaatcgtc tgtgccgctt aaatcgccat aaacttcatc ggtagaaata
     5881 tggtggaaac ggaaggcttc gtgcttttca gacggcattt gctgccggta ggcacgcgca
     5941 gcttcaagta ggttgaatgt gccgacgata ttggtttgga taaactcgcc tgccgaatcg
     6001 atagagcggt cgacatggct ttccgctgcc aagtgcatca cggcatcggg ccggtgttgt
     6061 gcgaatacgc ggtcgagttc ggcgcggtcg caaatatcca cttgctcaaa agcgtagcgg
     6121 ggattatcgg caatgtcggt cagcgattcg agattgcccg cgtaggtcag tttgtcgagg
     6181 ttgacgacgg aatcttgggt gttttgaatg atatggcgga caacggcaga gccgataaag
     6241 cccgcgccgc cggtaacgag gatggttttt ttgtttgcgg tttgcattta atccccatat
     6301 ccgttggggt tgcggctgac ccaacgccac gaatcctcca tcatttgcgc aaggctgcgt
     6361 ttggtttccc aacctgtctc ttgtttggta tgggacgggt cggcaaagga acacgccaag
     6421 tctccggcgc gtcggggttg gatttgatag ggaatattta agccggatgc ggcttcaaag
     6481 gcacggatga tttccaaaac cgaataggcg cgccccgaac ccaagttgaa caaatgtacg
     6541 ccggcaacac cgcctttcgc cttcattgcc gcgatatgcc cttctgccaa atccatcaca
     6601 tgaatatagt cgcgcattcc cgtaccgtcc ggggtcggat aatcgccgcc gaataccgac
     6661 agttgcggca gcctgcccga agccacttgg cagatatagg gcaaaaggtt gttgggaata
     6721 ccgttgggct gttcaccgat caagccgctt tcgtgcgccc caatcgggtt gaaatagcgc
     6781 aacaaaatca cgctccaacg cggatcggcc ttttggatgt cggttaacat ccgttccacc
     6841 atcgctttgg atgcgccata aggattagcg gtatcgcccg ggcgcatatc ttccgtatag
     6901 ggcattttac gtatcatggc gcgacgcatt aaagtaaacg ccaaagcgcg tttatactgg
     6961 ttatccagtt tccctatatt tttacgttac aaatcaagtt gttggtattc atcgtgaaaa
     7021 tagaattttt cggcataacg ctgactcata aaaccggcat gcgggctacc tgaaaaaatc
     7081 attgcgccat catcgtcatt taatgtttca tggttgtttt ctattaatgc taaagcaatt
     7141 tggtaattaa ttgccaaaat atcgttagta atcacacgat agccgcgttt tttggcttcg
     7201 taggcaaagg aacagcctcc actaaataca tctgcaactg tatctacatc agacggaagc
     7261 tggtcacaaa tccaggaagc tattttctct ttattaccga tatagttaat tttcggatat
     7321 tgcttattca ttttcactca tttttaaaat actttcagca atagcttttg ccaataaagg
     7381 cggtacggcg ttaccaacct gctgctgctg ggcaattttg ctgccgcaaa aaataaaatt
     7441 atcagggaaa gattgtaagg cagctaattc acgaacggtt aacgcccgat tctgttcata
     7501 gtgaaaaact ttgcgcatat ctcctgtaat acaaacggct ggttttgttg ctgttgtaac
     7561 ggatgtattt acggatatca cctgttttcg gacgtaatgg ttcaggaata tcgttacggt
     7621 tacctccatt tttaacaaat gccatttttt ctaacatttg tgccgaatga ttcatagctt
     7681 catgatttgc aacgtgtgga ttgctttcgc cagcagccag ttttggaaaa tgtcctattg
     7741 ctgatccaac agtctgatgg gaaatctgca aaggttcggg aaaggaaatt ttgcctttat
     7801 ccctcctccc gataaatatc actcggctac gtatctgagg aacaccgaaa tcggctgcac
     7861 tcagtatctt acattccacc gaataaccga tattctgaaa tgcttgaata atctcaatac
     7921 gtgttttacc tgaattgtgt gtatagagtc gcgctacatt ttccataaca aaaaaatatg
     7981 gttggacaat tttaactatt cggacaaact ctttaaataa atggttgcgt gggtcatctg
     8041 taaatgtccg tccaatcttt cctgccatac taaaaccttg acaaggtggt cctccaataa
     8101 tcaaatcaac tgcttgtccg ttaagacaat tgattaaatc ttgttcggtt agtgtggtta
     8161 aatctttttg cagtaattga tgatggggga agttggtacg gtaagtctga caataatcag
     8221 actccatttc aacagaaagc aattgttgga atccggcttg ttcaaaaccc aaggataggc
     8281 ctcctgctcc tgagaaaagg tcaatatagg taagtggtgt gtgcataagt caaaatccat
     8341 aaactcttcg attattttac cgcttcagct tcgcaaacgc atccgccatc gccgaattgg
     8401 ctggggcgcg gtcgttgcgt tggggtttgc ggccggctgt ccgttcgcgg ctgcggtttt
     8461 cagacggcat tttatgtttt gcgccgcccg gttcgtcatc caagcgcatg gtcagcgcga
     8521 tgcgtttgcg tgcagcatcg acttccagca ctttcacttt caccacgtcg ccagctttca
     8581 ccacttcgcg cgggtcttgg acgaacttgt tggacagggc ggagatgtgc accaagccgt
     8641 cctgatggac gccgatgtcc acgaacgcgc cgaagttggc gacgttggaa accacgcctt
     8701 cgagtatcat accgacttgc aagtcgctga tttcgtggat accttcggca aacgatgccg
     8761 tctgaaactc gccgcgcgga tcacggccgg gtttttccag ttcggacagg atgtccaaaa
     8821 tggtcggcag gccgaagcgt tcgtcggtga agtcggacgc tttgatttgc ttcacgcgct
     8881 cgcggttgcc gatgagttcg gcggcgctaa tgccttgttg cgccagcatt ttggcgacga
     8941 cgggataggc ttcggggtgg acggcgctcg cgtccaacgg ctctttgccg ccgttaatcc
     9001 gcaaaaagcc cgccgcctgc tcgaaggttt tttcgcccaa acgcggtact ttcagcaatt
     9061 ttttgcggct gtcgaacgcg ccgttttcat cgcggtaggc gacaatgttt tgggcaaggg
     9121 tttgattcaa accggaaatt cgcgccaaga gcggggcgga ggcggtgttc acgtccacgc
     9181 cgacggcgtt cacgcagtct tcgaccaccg cgtccagcga tttggcgagc tggctttggt
     9241 tcacgtcgtg ctgatactgc cccacgccga tggatttggg gtcgattttg accaactcgg
     9301 caagcgggtc ttgcagcctg cgggcgatgg acaccgcgcc gcgcagggaa acgtccaagt
     9361 cggggaactc gcgcgccgcc agttcggacg cggaataaat cgacgcgccg gcttcggaca
     9421 cgacgatttt atgcagcccc attttcgaca tttcacgcac cagttcgccc gcgattttgt
     9481 cggtttcacg gctggcggtg ccgttaccga tggcgatgag cttcacgccg tgctgtttaa
     9541 tcaggcgcga cagcgttgcc aacatattgt tttcttgatg caaatagacg atgacggtat
     9601 ccagcagctt gcccgtatca tccaccacgg cgcatttcac gccgttgcgg tagccggggt
     9661 cgagacccag cgtggtcagc cgtccggcgg gcgcgacgag cagcaagtct ttgagattgc
     9721 gggcgaacac ggtaatcgcg tcggtgtcgg cggcttcttt cagacggcct agggcttcaa
     9781 gttccaacga caaaaagatt ttcgcgcgcc aagtcagacg cacggtatcg cgcagccatt
     9841 tgtggccgtc tgaaaccttg aagcggcagg cgatgatttg ctcgtattcg ctttgctgcg
     9901 taatcggcgt gtcgtcgggc tgatatttga gcgcgatgtt caacacgcct tcgttgcgtc
     9961 cgcgcaaaac cgctagcgca cgatggctgg gcatcgcacg aataggttcg cggtggtcaa
    10021 aataatcgct gaatttttcg ccttcggttt ctttgcctcc aacaacttgc gcgtggattt
    10081 cggcttcgtt ccacagcttg tcgcgcagcg tgccgatgag ttccgcgtct tcggcaaact
    10141 gctccatcag aatcgcacgc gcgccgtcca acgcggcttt ggcatcgggg acgttttcgt
    10201 tcaggtagcc ttgtgcggcg gcttccacgt cctgcgactg ctcggcaagc aacacgtccg
    10261 ccagcggctg caaaccgtgt tcgcgcgcga tttgcgcttt ggtgcggcgt ttgggtttgt
    10321 agggcagata caggtcttcc agcgcggttt tgttatcggc ggcttcgatt tgcgccctga
    10381 ggtcgtctga aagcttgcct tgctcttcaa tgctttttaa cacaacggct ttgcgctctt
    10441 ccaactcgcg caggtattgc agccgctcgg caagccggcg cagctgcgtg tcgtccagcc
    10501 cgccggtggc ttctttacgg taacgcgcga taaacggcac ggttgcgccg tcgtctaaaa
    10561 gctcaatagc ggcattgatt tgcgcggtag tcgcggaaag ttcttgggaa agaatttgag
    10621 taatgttcat caatagaatt ccaacggaca ggccgtctga aatttcagac ggcctgattt
    10681 aaaaaacaat cgctttaagg cagcgaatta taatattcgt aggctttgtc catatcttca
    10741 aactggtgca tatgcccttt ttccagcacc atcgcattat cgcaatattg cttcatggcg
    10801 ctgtggctgt gcgacaccaa gatgatggaa cggtctttgc gcttttcaaa caactcgtac
    10861 ttacatttat cggcaaagcg cgagtcacca actgcaatca cttcgtcaat caaatagcaa
    10921 tcaaactcta ccgccaacga caaagcaaat gccaaacgcg ccttcatacc tgaagaataa
    10981 cgcttgaccg gctcgtaaag atattgcccc aactcggaaa actcttcggt aaagttcttc
    11041 acataatcga tatcgacatt gtaaatccgg cagatgaaac gcaaattgtc cataccggtc
    11101 agactacctt gaaacgcacc ggagaatgcc aaaggccaag aaatactcat tgtccgcttg
    11161 atttcacccg tggtcggcgg ctcgacacca ctgatcaaac ggatgagcgt cgatttacct
    11221 gcaccgttgc ggccgagaat accgattttc tcgcccttct ccattttgaa gctaatatcg
    11281 tgcaagactg tccgccaacc ttggcgggtc agatagcgtt tggaaacgtg ttcaactgaa
    11341 atcattgcgg ctcgactcct ttactgaatt tactgaccat cgccaagcca aacaacaaca
    11401 acaccagatt gcacaatacg atataccaag gattttcata ggtaattaca tcgctgccaa
    11461 aatatccggc acggaacatt tctgtgccat gcaccatcgg aatcattaat gcatattctt
    11521 gtaccttggg cggcaaatta tgcacaaaaa agaacgcacc ggataacggc atcatcacaa
    11581 aagtcaatgt gccccaaatc ttgccaaacg gctcgaaatt aaaggcaatc gaacaaatca
    11641 ccaaacccaa accaatcgca aaaaaagcca tcaaaagcca agccatcagc atataaaaca
    11701 tatctgccgg catttcaatc cagccaatcg caatcaatac cgccataatc acaatctgcg
    11761 caatggttgc accagcaatt tccaaaatca tgcgcgccaa gatggtatcc aaaactctta
    11821 cattgcggtg ataaagcaag ctggcatttg aagaaatcga cccaactgcc cgttttgagg
    11881 cattacgcca catcatcaac atcggatagc cagtaatcgc aaatgcgaca atattcaaag
    11941 ttgaatatcg gtctgccctt aaaaatttcc acatcaagac gataacgaat gtcatcagca
    12001 acggctcaac aaacagccat aaaaagccaa tattattgcg accgtaacgg gtgataattt
    12061 cccgcatcaa cagcgcaccg attacgcgcc tttgaatggc taaagattcc caaaatgatg
    12121 ttttatgcaa ggctttcatc agtttttatg ctcacgaatg ctggcagtca acaggctcaa
    12181 aataccataa accatcaagc cgataatcag agtggcaaca atgttgtata accgtttagg
    12241 ctcatgtgcc aaatccggca ggctcggttg cgagatcact tccaaataaa gctgctgacg
    12301 gtctgcttca accttggcac tttccaaaga agtcatggcg gctgccaact gctgctctgc
    12361 caactggttt tccaaataca cacgctgata ttcggcagcc tgattagaca acgaagaatg
    12421 cccaccgccc gaaatggcac gtaactgttg gtcaatttct ttacgcaagc tctgctcacg
    12481 cgcctgcaaa cccggaatct gcggattctc cggagtgact gctttcacct gatccagctg
    12541 ggtttgaatc acaatcaatt catcttgcag cttggaaacc aaccccattt gcacttccga
    12601 ttgcgctttc aaatcaaaaa cgccattggc aatccggtaa tccgtcagat tctgagaggc
    12661 ttcctttacc cgctctgccg ccgttttcac tacttcttcc gcatagcgca ccgtatcagc
    12721 acgtgcacga tcgttcaact ggttaatcaa tgcttcacct tgttttaaca aagcctcatt
    12781 gattttctta gattccagcg catcaaagga agttacattc aacgtggaaa tacccgaaac
    12841 cgtatcaaaa ttgatcatca cctgattttt atagtattga taaaaagcct cttcctcgcc
    12901 acggaaccca aacccattaa agcggctgaa cgcatcacct ttggtttcat aaaactcacg
    12961 caccggcaag attttacgca gttcatccaa agacgagcgc gaacgcatat actccccaac
    13021 cgtgtaaata tcatcttgcg cacgggcaaa acctgtgccc tgcaaaatgg cacccaggcc
    13081 attgagagaa gattggcttt taggcgagcg caccacaaag ctcgattgcg acgtaaaacg
    13141 atcggaagcg aagaagccga aatacaccaa cgaaattacc gtagggataa tcaccgttac
    13201 ccaaaataaa gggcttagct ttttaatcca acttttcttt ttcggctttt tacgctcggc
    13261 tttggtttca gtggcaactg ccacaggaag ttgctcagac atcttaatta ctcacattaa
    13321 ttagttaaat tattaatact gttcgcgcca ctggtaaccg gcgagaacac aaacgacaag
    13381 aatttctgca cttcagccaa cggcgcattc gacacataca atacatcttt attcttcaca
    13441 ggaaagcgct gcatagaaaa tagcgaatgc gcatcagcca tattcacacg atataccgtt
    13501 ggaatctctg cctcactgcc ataaccttga gcaatccatt tatcctgacg ttctgccggc
    13561 aattccacca atggcgtata gcggaacaca aacacaccac gcgcatcaga acggcgatct
    13621 tgcaaaccgc ccatacggcc aatggcttca gaaagcgata agcctctggc tgaaaaaccg
    13681 atttcttgtg ttctccccac cgcacccata gacgtaaagg tatagggatt ggtaatcatg
    13741 gtaaccacat caccgcgacg cagcaaaata ttttgtcgcg gatttgcaac taaatcttcc
    13801 aaggcaacag ttcgtactac attgccacgt gtcagctgca cattcgtatc ctgcacattt
    13861 gccgttgaac cacctaccgc agccaccgca tccaacacac gctcaccggc tgccgtcagc
    13921 ggcatacgca cactattgcc tgcacgaatc accgacacat tcgccgcatt attctgcacc
    13981 aaacgcacca tcacttgtgg ctgattggcc atttttttca ggcggccttt gatgatttcc
    14041 tgaacctgcc ctggcgtttt acccaccacc gaaatatcgc caacaaacgg cacagaaacc
    14101 gtaccacgtg ccgtgaccaa ctgctctggc aacttagttt gatgcgcact acccgagccc
    14161 atcgaagaaa ggccaccacc aaacaatact gccggcggcg cttcccaaat cataatatcc
    14221 aatacatcac caatatttag cgtaccagcc gaagcataac catcgccaaa ctgagtgaat
    14281 gactgattta tctgagcctt atataataac tgagcaaccg tatgattcac atcaatcagc
    14341 tccacttcag gaatttgaac ttcagattgt tgccctaaag agacaatttt ttttgcgctg
    14401 gggcctgatg aaggaatcgc agagcatcct acaattaaac ttccacacaa taataatact
    14461 gcgtgacgaa tataaaattt cactttaaac acaagccaaa tcctaatata attataaatg
    14521 gcctaattat agcacttaat cgaaataaat ttatgagtac gtagagtata attagtattc
    14581 ttctttccaa cttccttata cttatatact tatagattct aaaatcatga aaagaattct
    14641 ttgcattaca ggtaccagag ccgacttcgg caagctaaaa cctttattag cctatattga
    14701 aaatcaccca gaccttgaat tgcatttgat tgtaactggt atgcatatga tgaaaacata
    14761 tggcagaacc tgcaaggaag taactcgaga aaactatcaa catacatatc tgttttcaaa
    14821 tcaaatccaa ggtgaaccaa tgggtgccgt tttaggcaat accattacgt ttatctctcg
    14881 tctatctgat gaaattgaac ctgaaatggt catgattcac ggcgaccgtt tagaagcact
    14941 agcaggcgca actgtaggtg cattaagcag ccgtttagtt tgccatatcg aaggtggtga
    15001 actatctggt acagtagatg actccattcg tcattctatt agtaaacttt ctcatatcca
    15061 cttggtagca aatgaacaag ctgtcactcg cctagtgcaa atgggagaaa aaagaaagca
    15121 tattcacatc atcggctccc ccgatttaga tgttatggcc tcttccaccc tcccatcctt
    15181 agaagaagtc aaagaatatt acggtttacc atacgaaaat tatggtattt ctatgtttca
    15241 ccccgtgact acagaagcac atttaatgcc acaatatgcg gcccaatatt tcaaagcatt
    15301 agaattaagt ggccaaaata tcattagcat ctaccctaat aatgacactg gcactgaaag
    15361 tattctgcaa gaattattaa aatatcaatc tgataaattt attgcatttc cttccattcg
    15421 atttgaatat tttttagtct tattgaaaca tgctaaattt atggtcggca actcaagtgc
    15481 aggtattaga gaagctcctc tctacggtgt cccttcaatt gatgttggta cacgccaaag
    15541 taaccgccat atgggaaaat ctattattca tacagattat gaaactaaaa atatctttga
    15601 tgcgattcaa caagcatgca gtttaggcaa atttgaagca gatgatacct ttaatggcgg
    15661 agatactcgc accagcacag aaagatttgc tgaagtaatc aacaatcctg aaacgtggaa
    15721 tgtttctgct caaaaacgtt ttatcgattt gaatctttaa attatggaaa aacaaaatat
    15781 tgcggttata cttgcgcgcc aaaactccaa aggattgcca ttaaaaaatc tccggaaaat
    15841 gaatggcata tcattacttg gtcatacaat taatgctgct atatcatcaa agtgttttga
    15901 ccgcataatt gtttcgactg atggcgggtt aattgcagaa gaagctaaaa atttcggtgt
    15961 cgaagtcgtc ctacgccctg cagagctggc ctccgataca gccagctcta tttcaggtgt
    16021 aatacatgct ttagaaacaa ttggcagtaa ttccggcaca gtaaccctat tacaaccaac
    16081 cagtccatta cgcacagggg ctcatattcg tgaagctttt tctctatttg atgagaaaat
    16141 aaaaggatcc gttgtctctg catgcccaat ggagcatcat ccactaaaaa ccctgcttca
    16201 aatcaataat ggcgaatatg cccccatgcg ccatctaagc gatttggagc agcctcgcca
    16261 acaattacct caagcattta ggcctaatgg tgcaatttac attaatgata ctgcttcact
    16321 aattgcaaat aattgttttt ttatcgcccc aaccaaactt tatattatgt ctcatcaaga
    16381 ctctatcgat attgatactg agcttgattt acaacaggca gaaaacattc ttaatcgcaa
    16441 ggaaagctaa atgcaaaaca acaacgaatt taaaattggt aatcgttcag taggttacaa
    16501 ccacgaacca ttgattatct gtgaaatcgg catcaatcat gaaggctctt taaaaacagc
    16561 ttttgaaatg gttgatgctg cctataatgc aggcgctgaa gttgttaaac atcaaacaca
    16621 catcgttgaa gacgaaatgt ctgatgaggc caaacaagtc attccaggca atgcagatgt
    16681 ctctatttat gaaattatgg aacgttgcgc cctgaatgaa gaagatgaga ttaaattaaa
    16741 agaatacgta gagagtaagg gtatgatttt tatcagtact cctttctctc gtgcagctgc
    16801 tttacgatta caacgtatgg atattccagc atataaaatc ggctctggcg aatgtaataa
    16861 ctacccatta attaaactgg tggcctcttt tggtaagcct attattctct ctaccggcat
    16921 gaattctatt gaaagcatca aaaagtcggt agaaattatt cgagaagcag gggtacctta
    16981 tgctttgctt cactgtacca acatctaccc aaccccttac gaagatgttc gattgggtgg
    17041 tatgaacgat ttatctgaag cctttccaga cgcaatcatt ggcctgtctg accatacctt
    17101 agataactat gcttgcttag gagcagtagc tttaggcggt tcgattttag agcgtcactt
    17161 tactgaccgc atggatcgcc caggtccgga tattgtatgc tctatgaatc cggatacttt
    17221 taaagagctc aagcaaggcg ctcatgcttt aaaattggca cgcggcggca aaaaagacac
    17281 gattatcgcg ggagaaaagc caactaaaga tttcgccttt gcatctgtcg tagcagataa
    17341 agacattaaa aaaggagaac tgttgtccgg agataaccta tgggttaaac gcccaggcaa
    17401 tggagacttc agcgtcaacg aatatgaaac attatttggt aaggtcgctg cttgcaatat
    17461 tcgcaaaggt gctcaaatca aaaaaactga tattagttaa taataaaata gattaagcta
    17521 ttcttaaatt cagaatattg cttatctata ttaaaaattt ctaattttta aggttctgat
    17581 tgaaatcaga accttatttc aactattact ttttactcat aatcgaatta tatactttag
    17641 gactttataa tatggctgtt attatatttg ttaacggaat tcgggctgta aatggccttg
    17701 ttaaatcatc tatcaatact gcaaacgctt ttgctgaaga aggactggat gttcatttaa
    17761 ttaattttgt tggcaatatt actggagcag agcatttatc ccccccattc cacttacatc
    17821 ccaatgtcaa aacctccagc atcatagatt tatttaatga cattccagaa aatgttagct
    17881 gccgaaatat tcctttttat tctatccatc aacaattctt caaagccgaa tacagtgccc
    17941 actataagca tgttttgatg aaaattgaat ctttattatc tgaagaagat agcattatct
    18001 tcactcatcc tcttcaactg gaaatgtatc gtttagcgaa taataatatt aagtcaaaag
    18061 ccaagctaat tgtacaaatt catggtaact atatggaaga aatccataac tatgaaattt
    18121 tggcacgaaa tatcgattat gttgattatc ttcaaacggt atctgatgaa atgctggaag
    18181 aaatgcattc ccatttcaaa atcaaaaaag acaaattagt ttttattcca aacatcactt
    18241 atcccatttc attagaaaaa aaagaagctg atttctttat taaggataat gaagacattg
    18301 ataatgctca gaaatttaaa cgtatctcta ttgttggcag tattcagcca agaaaaaacc
    18361 aattggatgc cattaaaatc atcaataaaa ttaaaaatga aaattacatt ttacagatat
    18421 atggcaaatc tattaataaa gattactttg aattaattaa aaaatatatt aaagacaata
    18481 agttacaaaa ccgtatctta ttcaaaggtg aatcttccga gcaggaaatt tatgagaata
    18541 cagatatcct aatcatgaca tctcaaagcg aaggctttgg ttatatattt ctagagggta
    18601 tggtgtacga tatccctatc cttgcctata attttaaata tggagcgaat gattttagca
    18661 attataatga aaacgcttca gtttttaaaa ctggtgatat ttctggaatg gcaaaaaaaa
    18721 taattgagct attaaataac ccagaaaaat ataaagaatt agttcaatat aatcacaatc
    18781 gcttcttaaa agaatatgca aaagatgtgg ttatggctaa atatttcact attcttccgc
    18841 gcagctttaa taacgtatca ttatcgtctg ctttcagccg aaaagaattg gacgaattcc
    18901 aaaatattac tttttctatt gaagattcta atgatttagc tcatatttgg aatttcgagc
    18961 taaccaatcc tgcacaaaat atgaattttt ttgctttagt tggcaagcga aaatttccaa
    19021 tggatgctca tatccaagga acacagtgta cgattaagat agctcataaa aagacaggga
    19081 atttattgtc gcttttacta aaaaaacgaa atcagttgaa tttatcaagg ggatatacct
    19141 taattgcaga agataatagc tatgaaaaat atattggagc aatatctaat aaaggtaact
    19201 ttgaaattat tgcaaataaa aagaactcat tagttactat aaacaaaagt accttagagt
    19261 tgcatgagat tccccatgaa ctacatcaga ataaattact gattgcttta cccaacatgc
    19321 aaacgcctct aaaaattact gatgataatt taatacctat ccaagcctcc ataaaattag
    19381 aaaagattgg aaatacttat tacccatgtt tcttgccatc tggcatattt aataatatct
    19441 gcttagatta cggtgaagaa tccaaaatta ttaattttag taaatattct tataaatata
    19501 tctatgactc aattcgtcat attgagcaac atacagatat atcggatatt atcgtttgca
    19561 atgtttattc ttgggaactt attcgtgcct cagttattga gagccttatg gaatttaccg
    19621 gaaaatggga aaaacacttt cagacttctc ctaaaattga ttatcgattt gatcatgaag
    19681 gtaagcgttc gatggatgat gtcttttcag aagaaacatt tattatggaa tttccgcgta
    19741 aaaatggtat agataagaaa acagcagcct tccaaaatat accaaacagt attgtaatgg
    19801 agtatccgca gaccaatggt tacagtatgc gcagtcattc actgaaaagt aatgtagttg
    19861 cggcaaaaca ttttcttgaa aaattaaata aaattaaggt agatattaaa tttaaaaagc
    19921 atgaccttgc aaacatcaaa aaaatgaatc gaattattta tgagcattta ggcattaaca
    19981 taaatatcga agcatttcta aaaccacgat tagaaaaatt taagcgtgaa gaaaaatatt
    20041 ttcatgattt cttcaaaaga aataatttta aagaggtaat ttttccaagc acttattgga
    20101 atccaggtat tatttgtgct gcacataaac aaggtattaa ggtatctgat attcaatatg
    20161 ctgccattac tccttatcat cctgcgtatt ttaaatcacc aaaatcacat tacgttgctg
    20221 ataaattgtt cttatggtct gaatattgga atcatgagct tttaccaaat ccaacacgag
    20281 agattggttc tggtgccgca tattggtatg cattagatga tgtgagattt tcagaaaaac
    20341 tgaattatga ctatatcttt ctatctcaaa gtaggatttc ttcgcgcttg cttagttttg
    20401 caattgagtt tgcattaaaa aatcctcaac tacagctttt attttctaag catccagatg
    20461 aaaatataga tttaaagaac agaattattc ctgataatct tataatctcc acggaatctt
    20521 ctatacaagg catcaatgaa tctcgcgttg ctgtaggtgt ttattcaact agcttatttg
    20581 aggcattagc atgcggcaaa caaacttttg ttgttaaata tccgggatat gaaattatgt
    20641 caaatgaaat agattcaggg ttattctttg cagtagaaac acctgaagaa atgcttgaga
    20701 aaacaagccc gaattgggtg gctgtggcag atattgaaaa ccagtttttt ggccaagaaa
    20761 aataattttt aagttaatct ttaaaagtgc aatatttttc ttattttaaa tattgcactt
    20821 tattctttta atcataggaa ctcacatgta ttctgaacag ggaattaata atactattaa
    20881 catatctact acatctttaa ccaatgccac gcaactaact gtgatcggga ataataactc
    20941 tgtatatata ggtaataatt gtaaaatcgt cagctccaat attagattaa aaggaaataa
    21001 tataacctta tttatagctg atgatgtaga aatcatggga ctagtatgtt cccttcattc
    21061 ggactgctct ttacaaattc aagccaagac cactatgggc aatggagaaa ttaccattgc
    21121 tgaaaaaggt aagattagta ttggcaaaga ttgtatgctg gcacatggat atgaaattag
    21181 aaatactgat atgcatccaa tttattcttt agaaaatggc gaacgaataa accatggtaa
    21241 agacgttatt atcggtaatc acgtgtggct tggccgcaat gttactatat taaaaggtgt
    21301 atgtattcca aataatgtag ttgtaggttc tcatactgtg ctatataaaa gttttaaaga
    21361 acctaattgt gtaatagcag gttcacctgc aaaaatagta aaagaaaata ttgtatgggg
    21421 aagaaaaatg tatcattcaa ctatgtatga tgaccctact ttaaatgaat tttataaata
    21481 a
//
LOCUS       DAXIHS010000029        20741 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXIHS010000029 DAXIHS010000000
VERSION     DAXIHS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20741
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.029"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(1..152)
                     /locus_tag="ACFOF2_000956"
                     /pseudo
     CDS             complement(1..152)
                     /locus_tag="ACFOF2_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
     gene            complement(181..510)
                     /locus_tag="ACFOF2_000957"
     CDS             complement(181..510)
                     /locus_tag="ACFOF2_000957"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC7107299.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLAIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(588..806)
                     /locus_tag="ACFOF2_000958"
     CDS             complement(588..806)
                     /locus_tag="ACFOF2_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC7107300.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            928..1881
                     /locus_tag="ACFOF2_000959"
     CDS             928..1881
                     /locus_tag="ACFOF2_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC7107301.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIEAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPVVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1956..4013)
                     /gene="metG"
                     /locus_tag="ACFOF2_000960"
     CDS             complement(1956..4013)
                     /gene="metG"
                     /locus_tag="ACFOF2_000960"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC7107302.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPILPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4128..5966)
                     /gene="glmS"
                     /locus_tag="ACFOF2_000961"
     CDS             complement(4128..5966)
                     /gene="glmS"
                     /locus_tag="ACFOF2_000961"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC7107303.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6248..6766
                     /locus_tag="ACFOF2_000962"
     CDS             6248..6766
                     /locus_tag="ACFOF2_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107304.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
     gene            complement(7016..7078)
                     /locus_tag="ACFOF2_000963"
                     /pseudo
     CDS             complement(7016..7078)
                     /locus_tag="ACFOF2_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7184..8509)
                     /locus_tag="ACFOF2_000964"
     CDS             complement(7184..8509)
                     /locus_tag="ACFOF2_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC7107305.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            8721..9560
                     /gene="efeU"
                     /locus_tag="ACFOF2_000965"
     CDS             8721..9560
                     /gene="efeU"
                     /locus_tag="ACFOF2_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC7107306.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            9557..10723
                     /gene="efeO"
                     /locus_tag="ACFOF2_000966"
     CDS             9557..10723
                     /gene="efeO"
                     /locus_tag="ACFOF2_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC7107307.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            10987..12252
                     /gene="efeB"
                     /locus_tag="ACFOF2_000967"
     CDS             10987..12252
                     /gene="efeB"
                     /locus_tag="ACFOF2_000967"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC7107308.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(12324..12653)
                     /locus_tag="ACFOF2_000968"
     CDS             complement(12324..12653)
                     /locus_tag="ACFOF2_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC7107309.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(12730..14100)
                     /gene="glmU"
                     /locus_tag="ACFOF2_000969"
     CDS             complement(12730..14100)
                     /gene="glmU"
                     /locus_tag="ACFOF2_000969"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC7107310.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(14155..14427)
                     /locus_tag="ACFOF2_000970"
     CDS             complement(14155..14427)
                     /locus_tag="ACFOF2_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC7107311.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPIYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(14403..15134)
                     /locus_tag="ACFOF2_000971"
     CDS             complement(14403..15134)
                     /locus_tag="ACFOF2_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC7107312.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMTRRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPRKYERMLCVKPSLS"
     gene            15193..16194
                     /locus_tag="ACFOF2_000972"
     CDS             15193..16194
                     /locus_tag="ACFOF2_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7107313.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            16288..17136
                     /locus_tag="ACFOF2_000973"
     CDS             16288..17136
                     /locus_tag="ACFOF2_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC7107314.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            17162..17647
                     /locus_tag="ACFOF2_000974"
     CDS             17162..17647
                     /locus_tag="ACFOF2_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC7107315.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(17749..19317)
                     /gene="msrAB"
                     /locus_tag="ACFOF2_000975"
     CDS             complement(17749..19317)
                     /gene="msrAB"
                     /locus_tag="ACFOF2_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC7107316.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            19463..20728
                     /gene="ftsY"
                     /locus_tag="ACFOF2_000976"
     CDS             19463..20728
                     /gene="ftsY"
                     /locus_tag="ACFOF2_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC7107317.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4422 a         5188 c         6203 g         4928 t
ORIGIN      
        1 ttgtcgctgg cggcggtaac ggcggtgtcg gtgtcggtgt cggcgcgcgt aaccggctgt
       61 ccgcagaacc attttaccga accgtcttga cgcttggccc acagggagag ttttttgtct
      121 ttgatttcgt tgtttacgcc gcttgaagcc attatgtcag acggtattgc ccgggcagct
      181 ttattcgtac actttcagca gctcgacttc aaatatcaaa gtggcgtgcg ggggaatcac
      241 gccgcccgcg ccgtgtgcgc cgtagcccat ttccgaaggg atggccagct tgcgtttgcc
      301 gccttccttc atgccgccga agccttcgtc ccagcctttg atgacttgtc cgacaccgag
      361 cgtgatggtc agcggctggc ggcggtcgag gctggagtcg aatttggttc cgttttccag
      421 ccaacctgtg taatgcacgg taatctcttt gcctttaact gcttcttttc cgaagccttc
      481 ttgcaagtct tcaataatca ggccgcccat atttgtcctt tcgttgcttg ttggtcaaaa
      541 cggcaagggt aacataccgt ccgtcgaagt caaatgccgc tcaaacgtca gctgcatcgg
      601 tgcagctgaa acggctgtgt ttgttggact gttttatttt tttcgtaaag gttccatgct
      661 ttttcatgga aatagaaaac gacggtgttg attaggggct cgaccagcgc gaccgccccc
      721 gatacgccta tactgcccgt cagtacatag gttacactga aggcggtgct gaaatgcagt
      781 gcggcaaaag tcagggtttt aagcatcatc ctctcccgaa ttggacattg acggaggaat
      841 gataaaggtt atcataaggt tgcgcggttt aaatttgcta tttgttgtta gtgtaaataa
      901 atcatttttc aaatggggat agaaattatg aatcataaaa agatcgttgt tttggatgcg
      961 gatactttac ccggccgggt ttttcatttt gattttccgc acgagcttgc ggtttacggt
     1021 acgacaggtg cggatgaaac ggcagaacgg gtgcgcgacg cgcatattat catcaccaat
     1081 aaagtgatga tttcagcgga tatcattgca cacgctccgc aacttgaatt gattgccgtc
     1141 agtgcgaccg gtgtgaacaa tgtcgatatt gaggcggcga aggcggcggg cgttgcggta
     1201 tgcaatgtcc gcgcctacgg aaacgaatcg gttgcggaac acgcctttat gctgatgatt
     1261 gcgttaatgc ggaatttgcc cgcctatcag cgtgatgttg cggcaggatt atgggaaaag
     1321 tcgccgtttt tctgccatta tggcgcgccg attcgggatt tgaacggtaa aacgctggcg
     1381 gttttcggac gcggcaatat aggacggacg cttgcccgat atgcacaggc attcggtatg
     1441 agggtagtgt ttgccgaaca caaacacgcg cccgttgtgc gtgaaggcta tgtttccttt
     1501 gaagatgcgg tacagactgc cgacgtgttg tcgctgcact gtccgctgaa cgcccaaact
     1561 gaaaatatga taggcgaaaa cgaattacag cagatgaagc ccggtgcggt tttgatcaat
     1621 tgcgggcgcg gtgggctggt ggatgaaaat gcgctggttg ccgcactcaa atacgggcag
     1681 attggcgggg caggtgtcga tgttttgacg gaggaaccgc ctaggggcgg caatcccttg
     1741 ctgaatgcac gacttcccaa tctgattgtt acgccgcata ccgcgtgggc aagtcgtgaa
     1801 gcgttggaca ggctgttcga tatattgttg gcgaacattc acgcctttgt gaaaggcgag
     1861 gcgcaaaacc gcgtggtttg aacctgtcgg gattgcggaa aaaaatgccg tctgaacgcc
     1921 tcaagggttc agacggcatt ttttgagatt tctgcttaac cgactttgtc gcccggttgc
     1981 gcgcctgcat ccacatccaa gagcttcagt ttcccgtctg ccgtggcggc agagagaatc
     2041 atgccttcgg atacgccgaa ttttgccatt ttgcgcgggg cgaagttggc gacggcgatg
     2101 accatccttc cgtttaactc ggcagggttc aggtaagacg cggcgatgcc ggagaagatg
     2161 atgcgttttt caaaaccgaa atcgaggtcg aatttcaaaa gtttggtgct gccttcgaca
     2221 gcttcgcagt tcaatacttt ggcaacgcgc atatcgattt tcataaagtc gtcgaagctt
     2281 gcctgttcgg caactttttc gtattgactt tcttcggcgg caggtgcggc tgcggcggcg
     2341 atgctttgtt tgttggcttc gattaaatcg tccacttgtt tttgctccac tcgttgcatg
     2401 agatgttcgt atttgttgat ggcgtgttcg cccaaggttt cgcgtgtgtt tgcccaagtg
     2461 atggcttcca aattcaggaa tttggcggcg ttttcggcca ctttcggcaa gatgggggca
     2521 aggtaggcgg tcagcatggt aaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt
     2581 tcgtcttggc cttcttgttt cgccagttcc caaggtttgt tggcatcgac gtattcgttg
     2641 acaatgtctg ccaaggccat gatgtcgcgc agggcgcggg cgtattcgcg gttttcgtat
     2701 tgctcggcga tggtgtcgct ttcggcggca agttttgcca gcaatgcgct gcctgaaacg
     2761 tctttcagac ggccttcaaa gcgtttggcg atgaaacctg atgcgcgggc ggcgatgttg
     2821 acgtatttgc cgacgaggtc gctgtttacg cgcgaaataa agtcttgcag gttcaaatcg
     2881 atgtcttcga ttttgctgtt gagttttgcg gcgatgtagt agcgcatcca ctcggggttc
     2941 aggccttgtt ccaaataaga tttggcagtg atgaaggtgc cgcgcgattt ggacattttt
     3001 tgtccgtcta cggtcaaaaa gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag
     3061 aaatgcagca tggcaggcca gaacagggcg tggaaataga ggatgtcttt gccgatgaag
     3121 tggtacatct cggtttggct gtcggctttg aagtattcgt caaaatcgac gccgatgcgg
     3181 tcgcacaggt ttttaaacga cgccatgtag ccgacgggcg cgtccagcca gacgtagaag
     3241 tatttgcccg gcgcgtcggg gatttcaaaa ccgaaatacg gtgcgtcgcg ggaaatgtcc
     3301 cagtcggaca gggtggtttc ttcgccttcg cccagccatt ctttcatttt gttgagggct
     3361 tcgggttgca gatggggctt gccgtcgtgc ggattgttgc cggaagtcca tgctttgagg
     3421 aagtcggcgc attcgtccag tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttg
     3481 gcaccggaaa cggcggaata cggattaatc agttcggtcg gggaataggt cgtgccgcag
     3541 acttcgcagt tgtcgccgta ttggtcttgg gcgtggcatt tcgggcattc gcctttaacg
     3601 aaacggtcgg gcaggaacat ttgtttttca gggtcgaaaa gctgctcgat gacgcggctt
     3661 tcgattttgc cgttggcttt cagcgcgcgg taaatgtctt gggaaaactg tttgttttca
     3721 ggggaatggg tgctgtaata attgtcgtag ccgatgttga aaccggtaaa gtcggcgagg
     3781 tgctcttcgc gcactttggc aatcatgtct tcgggcgcga tgccttgttt ttgcgcggca
     3841 agcatcacgg gcgtgccgtg ggtgtcgtcg gcgcagcagt agtagcattc gtggccgcgc
     3901 agtttttgga agcgcaccca aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg
     3961 atgctgccgt tggcataggg cagggcggag gtaactaaga ttttgcgtgt catattgtgc
     4021 tttgcaaaca atgggtaaag gcggattata ccgcaaatca aacggggaaa tgccgtctga
     4081 agcctgaaaa atcgggcttc agacggcatt ttgccaatcg gcgggattta ttcgacggtt
     4141 acggatttcg ccagattgcg cggcttgtcc acatccgtgc cgcgtgcgag ggcggtgtgg
     4201 taggcgagga gctgcacggg gatagtatgc acgacggggg acagtttgcc gacgtggcgc
     4261 ggtgcgcgga taacgtgcac accttcggtg gcattaaaat tgctgtcgag gtcggcaaag
     4321 acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat tggctttgac tttgtccaac
     4381 aggctgtcgt tgggtgcgat gacgacgacg ggcatgtttt cgtccaccaa ggcaagcggc
     4441 ccgtgcttca gttcgccggc aggataggct tcggcgtgga tgtaggtgat ttccttcagt
     4501 tttaacgcgc cttcgagggc aatcgggtaa tggatgccgc gccccaaaaa cagcgcgctg
     4561 gttttcttgg caaactgttg cgcccaagcg gcaatttgcg gttcgaggtt taaagcgtgc
     4621 tgcacgctgc cgggaagctg gcggagttct tcggtataac gtgcttcatc ttcgccggat
     4681 accaagccgc gtaccttcgc cagcgttacc gccaaaccga acagtgcgac cagctgcgtg
     4741 gtaaatgctt tggtcgaggc gacgccgatt tccgcaccgg cacgggtata aagcacgagg
     4801 ctgctttcgc gcggcagggc ggattccatc acgttgcaaa tggagaggct gtggcggtgt
     4861 cccaaagatt tggcgtattt caaagcctcc atcgtgtcca gcgtttcgcc ggattgggaa
     4921 atggtaatga ccagttggtc cggatcggca atcacgctgc ggtatcggta ttcgctggcg
     4981 atttcgacgt cggtcggaat tttggcgatg gattcgagcc agtatttggc ggtcagcgcg
     5041 gcgtaatagg acgtgccgca ggcaaggatt ttgacgctgc ggatgctttc aaacacgctt
     5101 ttggcgtttt tgccgaagtt ttcggggatg aagccgccgt cgaggaaaac ctccgccgtg
     5161 tctgcaatcg cgcggggctg ctcgtggatt tccttttgca taaagtggct gtacggcccc
     5221 agctccaaag aggcgagcga gagttcggat accttgactt tgcgttcggc aggcaggccg
     5281 tttttatcgg tcagcctttt gatgccgtct gaagccagca gtgcgatgtc gccgtcttca
     5341 aggtacgcca cgcggcgcgt aaaggcgatg acggcggaca cgtccgaagc gataaaggtt
     5401 tcatcgtcgc ccaaagcgac caaaagcggg cagcccatac gcgccacaac taattcatca
     5461 ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt ggaaacgttt gaccgcttcc
     5521 tgcacggctt caaacagcct gccgccgttt tgcgcgtatt cgtgattgat gctgtgggcg
     5581 atgacttcgg tatccgtttg cgattcaaac cggtatccca aaccttccaa gcgtttgcgt
     5641 tcgctttcaa agttttcgat gatgccgttg tggacgacgg ctatcatccc accggaaata
     5701 tgcggatggg cattcggctc ggtaacgccg ccgtgtgtcg cccaacgcgt atgcccgatg
     5761 ccgatgccgc cgctgatgcc tttttcgcgt gctgcgtctt ccataagctg cacgcgtccg
     5821 acgcggcgca cgcgtttgat tttgccgtcg gtgttgacgg caatgcctga tgagtcataa
     5881 ccccggtatt cgaggcgttt gaggccgtcg gtcagaaaat cgacgacgtt gtgatgggcg
     5941 cggatggcgc cgacgatacc gcacataact gttccttagt atccggttga aaaaaaacag
     6001 gcgcggacgg ctcccgtgcc gcaccttcct cttcggatta taaaccgcct cccgcgccga
     6061 aaaacagcaa aatgccgtct gaaggcttgg gcttgctcaa aaaagggagg gatttccccg
     6121 cctgaccggg atgggcgttc agacggcatt gcctgccgcc ggtttatagt ttttacaaat
     6181 caatattgac aagctgaaaa aaaaaaacaa tatactcgct cggtcttaat gttaacggag
     6241 tatggaaatg aaacaaatgc ttttagccgt cggcgtggtg gcggtgttgg cgggctgcgg
     6301 caaggatgcc ggcggttacg agggttattg gcgcgaaaaa tcggacaaaa aagagggtat
     6361 gattgccgtc aaaaaagaaa aaggcaatta cttccttaat aaaatcaacg tgtttacagg
     6421 taaggaagaa tctatgcttt tgtctgaaaa agacggcgcg ctttcgataa acacggggat
     6481 aggggaaatc ccgatcaaac tttccgatga cgggaaagag ctgtatgtcg aacgcaggca
     6541 gtatgtcaaa accgatgcgg cgatgaagga caaaatcatc gcccatcaga aaaagtgcgg
     6601 acaaacggca caggcatatc tcgacgcgcg aaatgcgttg ccgtcaaacc agacgtatca
     6661 gcagcatcag gcggcgatcg agcagttgaa acggcggttt gaagccgagt ttgacgaatt
     6721 ggaaaaagaa atcaaatgca acggcaaacc gacattgttg ttttagcgta ggacaaccgg
     6781 gaggatgccg ccgtccgaat cggacgtgcg gtttctgtac cggtacgggc gggcaggaat
     6841 gtccgctttt tttgttcgga cgcgtttgaa tacccgtttg attccgtccg tttgcaaagg
     6901 gtatttttgt tcgggcggaa attatagtgg attaacaaaa accagtacag cgttgcctcg
     6961 ccttagctca aagagaacga ttctctaagg tgctcaagca ccaagtgaat cggttccgta
     7021 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaatttgat ccactataat
     7081 tccgtcaaat aagaaaggaa ttttgtgcct gcggtatcgc aaaacttcgc cttaatgcgc
     7141 ccgattgcct agggatgggc ttcagacggc attgttttcc ggtttacggg cggtattcgg
     7201 gcttcatacc gttgggcaga agctgccaga catatcccgt ggttttctgt ttgccggcaa
     7261 gttcgccggc ttcgtcgccg taaccccaaa aataatccac gcgcaccgcg cctttaatcg
     7321 cgctgccggt atcctgcgcc ataatcaggc ggttgagggc tttgcgggta accggatggg
     7381 cggtggcgac aaataagggc gcgcccaagg taatgtagtg ccggtcgact gcgccggcgt
     7441 actcgcccat cagcggcgtg cccagtgcgc cgacagggcc gtcattgctg cttccggtaa
     7501 gctctcggaa aaagatatag ctggggtttt gccccaaaac ttcggcgagg cgttgcgggt
     7561 tttgctgcat ataggctttg atgccctgca tcgaggtctg cccgagcttg aggtagcctt
     7621 tgtccgccat atagcgtccg atggaaacgt agggatgttc gtttttgtcg gcatagccga
     7681 tgcggatgta tttgccggac ggggttttca gacggcccga gccttggatg tgcataaaaa
     7741 aaagttcgac ggggtcttcg gcgtaaccga gtatcggggc tttgccgtca agcgcgccgc
     7801 cgttgatttg gttgcgcgtg tggtagggga ggaagcggct tccttcaaac ctgcctttga
     7861 ttgccgttgt gcgcgcggtg atggggaatc gggagaggtc ggcggtgtgc gtgccgccgg
     7921 cattgtcgat tgtgccgctg tttttccccg tctgcctgat gcggacaagg gcttttccgc
     7981 tccgcaaacc ggcaggcagg gggacggaga taaaatcgtc gggaataccg taaatcggga
     8041 agcgggcttg tgccgtccgc ctgtcgtcgc ccttcagcac cggctcgtaa tagccggtaa
     8101 ccgtaccggc aaggcttccg ttgcctgcaa cctgccacgg cgtgaaatag cgttcaaaaa
     8161 actgttttgc ctgaaaggaa tggacggggg tttgaaaggc ttgggcacac acatcctgcc
     8221 agccttggcg gtttttcaaa ttggcgcagc cgaggcggaa ggattgcagg cttttggcga
     8281 aatcctgcgc cgcccagtgg ggcagggaca ggtgcggcac aacggtataa acggccccgc
     8341 cgccggcaac cgtcgttccg gcgggggcgg ggatgccgac cggccggtcc gggccgttga
     8401 tgacggatgt gtcgggttgc ggaaaggttt ggatgctctt gctttggcag gcggcgagga
     8461 tggcggcggc gatgccgcac agggcggcgc ggaacaggta ttttttcata atggacaatg
     8521 ttgtcagcag taataaaaaa agatggtttc gggcggcgtt gcggcagccg tggagagggg
     8581 attttaacac agggcgcagc tgcagcctgc ggaactttcc gccgcgcggt actgcagata
     8641 aaaataactt gcatttgtat ttacaagcaa tgaaaatatt ccgataatat attattcatc
     8701 atccttgttc gttcgcgttt atgctggtcg cttttttaat tatgttgcgc gagggtatcg
     8761 aagccgcgct catcgtcggc atcgttgccg gttttctgaa acagtccgga cattccaaac
     8821 tgatgcctaa ggtctggttc ggggtcgtcc ttgcttcttt gatgtgtttg ggcatcggat
     8881 acggcatcca ttcggcaacg ggcgagattc cccagaagca gcaggagttc gtcgtcggca
     8941 ttatcggttt ggttgctgtc gcgatgctga cttatatgat tttatggatg aaaaaggcgg
     9001 cgcgttcgat gaagcagcag cttcaggatt ctgtacaggc ggctttgaac cgtggcagcg
     9061 gtcaaggatg ggccttggtc ggtatggcgt ttcttgccgt ggcgcgtgaa ggtctggaga
     9121 gtgttttttt cctgcttgcc gtattcaaac agagcccgac ttggcagatg ccggccggcg
     9181 cggtagcggg ggttttggct gccgccgtga ttggcgcgtt gatttatcag ggtgggatgc
     9241 gcctgaatct ggcgaagttt ttccgttgga cgggggcgtt tctgattgtc gttgccgccg
     9301 gcctgcttgc cggttcgctg cgcgcgctgc atgaggcagg tatttggaac gcgcttcagg
     9361 acattgtgtt cgactcatca aaatatttgc acgaagacag cccgttgggc gtgctgctcg
     9421 gcggattttt cggctatacc gaccatccga cgcagggcga agccttggtt tggctgctgt
     9481 accttattcc cgtcatgact tggtttttgt gcggcagcag gccgtctgaa actttaaccc
     9541 gtaaagagga gctgaaatga gaaaattcaa tttgaccgca ttgtccgtga tgcttgcctt
     9601 aggtttgacc gcgtgccagc cgccggaggc ggagaaagct gcgccggcag cgtccggtga
     9661 ggcgcaaacc gccaacgagg gcggttcggt cagtatcgcc gtcaacgaca atgcctgcga
     9721 accgatggaa ctgaccgtgc cgagcggaca ggttgtgttc aatattaaaa acaacagcgg
     9781 ccgcaagctc gaatgggaaa tcctgaaagg cgtgatggtg gtggacgagc gcgaaaacat
     9841 cgcccccgga ctttccgata aaatgaccgt caccctgttg ccgggcgaat acgaaatgac
     9901 ttgcggtctt ttgaccaatc cgcgcggcaa gctggtggta accgacagcg gctttaaaga
     9961 caccgccaac gaagcggatt tggaaaaact gtcccaaccg ctcgccgact ataaagccta
    10021 cgttcaaggc gaggttaaag agctggtggc gaaaaccaaa acctttaccg aagccgtcaa
    10081 agcaggcgac attgaaaagg cgaaatccct gtttgccgac acccgcatcc attacgaacg
    10141 catcgaaccg attgccgagc ttttcagcga actcgacccc gtcatcgatg cgcgtgaaga
    10201 cgacttcaaa gacggcgcga aagatgccgg atttaccggc ttccatcgta tcgaatacgc
    10261 cctttgggtg gaaaaagacg tgtccggcgt gaaggaaatt gcagcgaaac tgatgaccga
    10321 tgtcgaagcc ctgcaaaaag aaatcgacgc attggcgttt cctccgggca aggtggtcgg
    10381 cggcgcgtcc gaactgattg aagaagtggc gggcagtaaa atcagcggcg aagaagaccg
    10441 gtacagccac accgatttga gcgatttcca agccaatgtg gacggttcga aaaaaatcgt
    10501 cgatttgttc cgtccgctga tcgaggccaa aaacaaagcc ttgttggaaa aaaccgatac
    10561 caacttcaaa caggtcaacg aaattctggc gaaataccgg actaaagacg gttttgaaac
    10621 ctacgacaag ctgggcgaag ccgaccgcaa agcgttacag gcctctatta acgcgcttgc
    10681 cgaagacctt gcccaacttc gcggcatact cggcttgaaa taagccgcaa gcgttcagac
    10741 ggtatttggc ggcagatacc gtctgaagtt ttatagtgga ttaacaaaaa ccagtacggc
    10801 attgcctcgc cttgccttgc cgtactattt atactgtctg cggcttcgtc gccttgtcct
    10861 gatttttgtt aatccactat atccgccata tattgcaggg cgggatttca acctgccgct
    10921 atcggttaat ggaaaaacgg cgtgcaggga tacccatcct gctgcacgga tattgaagga
    10981 aacaccatga gcaaaaacca acccgcacaa ccgaccaggc gcactctttt taaaaccgcg
    11041 atcgcagccg gagcagtcgg cgcaatcgga ggttatctcg gcggcaaaaa acggggcgaa
    11101 accgccgaac gcaccgccga aagtcaacac tcgccccaag cctatccctg ctacggcgaa
    11161 catcaggcag gcatcgttac gccgcagcag gcgttttcga ttatgtgcgc cttcgacgta
    11221 accgcgcaaa gtgccaagca gctggaaaac ctgttccgca cgctgaccgc ccgcatcgag
    11281 tttctcaccc aaggcggcga ataccaagac ggcgacgaca aacttccgcc agccggcagc
    11341 ggcattttgg gcaaagcctt caaccccgac gggttgaccg ttaccgtggg ggtgggcagc
    11401 agcctgtttg acggccggtt cggactcaaa gacaaaaaac cgattcattt gcaggaaatg
    11461 cgcgacttct ccaacgataa gctgcaaaaa agctggtgcg acggcgattt gagcctgcaa
    11521 atctgtgcct tcacccccga aacctgccaa gccgccctgc gcgacatcat caaacacacc
    11581 gtccaaaccg ccgttatccg ctggagtatc gacgggtggc agcctaaatc cgaacccggc
    11641 gcgatggcgg cgcgcaacct gttgggcttc cgcgacggca cgggcaaccc caaagtttcc
    11701 gaccccaaaa ccgccgacga ggttttgtgg acgggtgtgg ccgccaacag cctcgacgaa
    11761 ccggagtggg cgaaaaacgg cagctatcag gcagtccgcc ttatccgcca ctttgtcgag
    11821 ttttgggaca ggacgccgct tcaagagcaa accgacattt tcgggcggcg caaatacagc
    11881 ggtgcgccga tggacggcaa aaaagaagcc gaccaaccgg attttgccaa agaccccgag
    11941 gggaatacca cgcccaaaga cagccatata cgcctggcga atccgcgcga tcccgaattc
    12001 ctcaaaaaac accgcctctt ccgccgcgcc tacagctatt cgcgcggact cgcctcaagc
    12061 ggacagcttg atgtcgggct ggtgttcgtc tgctatcagg caaaccttgc cgacggattc
    12121 atcttcgtgc aaaacctcct caacggcgaa ccgctggaag aatacatcag ccccttcggc
    12181 ggcggctatt tcttcgtctt gcccggcgtg gaaaaaggcg gctttttggg gcaagggctg
    12241 ctgggcgtat aaatccgcca tataaaaaac gccgtccgaa ccttgccaaa cgggttcgga
    12301 cggcgtttct tgtttttggg cggtcaggct tttttgacga attcggattt taaattcatc
    12361 gcgctgccgt cgattttgca gccgatgttg tgatcgcctt cttgcaggcg tatgcctttg
    12421 acttttgtgc cttgtttgat caccatcgag ctgcctttta ccttgaggtc tttgatgagg
    12481 atgacggtat cgccgttttg cagcactgcg ccgttggcat cgagcacttg agccgcaagg
    12541 tcggcggcgg attcggtttc attccattcg tgggcgcatt cggggcagat gtattgtccg
    12601 ccgtcttcat aagtgtattc ggaggcgcat tgtgggcatg ggggtaatga catggtttgc
    12661 agtccttatc tgatatcgga ggagtttgat gccgtctgaa acctgaaacc ggcttcagac
    12721 ggcatagctt tattgcttgt ctttttcagg ccgcatccag ccttcgatga cggtttggcg
    12781 ggcgcgggcg agggcgagtt tgttgtcttc gatattgcgg gtaatcgtgc tgcctgcgcc
    12841 tgtggttact ttgttgccga gggtaacggg ggcgactagg acgcagtttg aaccgatgcg
    12901 gacttcgtcg ccgataacgg ttttgtgttt gtgcacaccg tcgtagttgg caataatcgt
    12961 acctgcgccg aagttggttt tgctgccgac ttcggcatca ccaatgtagg tgaggtggtt
    13021 ggctttggtg cctttgccga tggcggcgtt tttgatttcg acgaagttgc cgacgtgtac
    13081 gtcggcggct agtttggctt gcggacgcag gcgggcatag gggccgattc ggttgttttc
    13141 gccgacttcg cagctttcga ggtgggagaa gggggcgatt ttgctgtttg cgccgatttt
    13201 ggcgtttttg atgacgcagt ttgcgccgat ttcgacgttg tcgccgagtt cgacttcgcc
    13261 ttcaaagata cagttcacat caattacgac atcctgtccg tgtttcagac ggcctcgtaa
    13321 atcgaaacgt gccggatcgc gcagggttac gcctgctttg agcaattctt gcgcctgttc
    13381 ggtttggaag atgcgttcga gttcggcgag ctggcgtttg ttgttcacgc cggcggcgag
    13441 gtgggaggcg cggacttgga cgggatgaac tttaataccg tcggcaacgg ctttggcaat
    13501 gaggtcggtt aaataatatt cgccttgagc attgttgctg gagaggctgt tcagccagtt
    13561 ttccagtttg gcgttgggca gaaccaaaat gccggtattg atttctttca cggctttttg
    13621 ggcggcgtcg gcgtcttttt cttcgacaat ggcggttacg ctgccgctgc cgtcgcggat
    13681 gatgcgcccc aaacctgacg ggtcggcggg cacgtcggtc aacagcccga cttcgttgcc
    13741 tgcggcttcg agcagggttt cgagggtttc aacgtcaatt aaaggaacgt cgccgtacaa
    13801 caccagcgtg cggccttcgg cggcaaggtg gggcagggcg gttttgacgg cgtggccggt
    13861 accgagctgt tcggtttgtt caacccaaac gacatcgcgt ttgacagcgt ttaaaacttg
    13921 gtctttgccg tgcccgatga cgacgcagat gttttgcgga ttcagcgcgg cggcggtgtc
    13981 gataacgcgc ccgagcatgg gcttgccgcc gatgcggtgc agcactttgg gcattttgga
    14041 atacatgcgc gtgcctttgc cggcggcgag gataacgatg tttaaagtgt tttgcggcat
    14101 gacggtttcc tgtgcaatgc cgtctgaaag ggcttcagac ggcatagggt gggtttatcg
    14161 gttttgaaat ttgggctttt gccagtgttg gcgatgctct tcgtcggcgt tgttgccggt
    14221 ttggttgggt aacacggcat ggcgttcggg acggtattgg ttgtagttca tatttttcga
    14281 gtagctgccg tcttggtaat aaatgggcgt gccggcggga tatttttgac ggacggcggt
    14341 cttgccgttg ccgtcttgat aagtttccca cgcgcagccc gacaaaaggg cggcggcagt
    14401 catcaggata aggaaggttt tacgcataac attctttcgt attttcgggg ggttgtgcgg
    14461 gtattgtaat gattttggcg gtgttctgac aaagtttctg cataccgagc cagttgcgcc
    14521 atatcgctta cggaagcatc gataaagggc aggtcgtgca cttttgcacc gaaccggacg
    14581 gttttcatgc ccaatgcttt tgcctgatgc aggttgtccg cgctgtcgtc caccataatg
    14641 cagcattcgg gcggtacgtc caacaggcgg cagacattga gatacgcttg cggattgggt
    14701 ttgtacagca gcccgaaatc atccgtgccg aaaagcgcgt cgaaacggtt ttccaaaccg
    14761 agtgcgccga caacggcacg gacgtaaaac gacgggccgt tggaaaaaac cgccttgcgc
    14821 ccttttaggc ggctcagggt gttttgtgtt tccggcatac cgtgcagacg ggtcaaaacc
    14881 tcgtcaatcg gatggctttc gcgcaaaaat tcggcgatgt cgatttcggg atggtggatt
    14941 tgcagtccgg cgagcgttgc gccgtagcgg tgccaatagt cttgacgcag gtcggacgcg
    15001 gcggattcgg agagtttgag gcggcgtgtc atatagcgtg tcatagcgcg gttgatgagt
    15061 gtgaagaggc cggcatcggc attgtgcagc gtgttgtcga ggtcgaacag ccacacggtc
    15121 gggttttctt gcattttgaa ccgtgaaaat ttgttagaat gttattttac agcgaataga
    15181 ggaggactcg gaatgaaacg gaaaatttgg ctgctgccgc tgctggcggt ttcggcatac
    15241 ctgcaggcgc agacggaagt caggctggcg gtgcataagt cgttcagcct gcccaaaggg
    15301 ctgattgcgc gcttcgagcg ggcaaacgat gcgaaggtgt cgattattca ggcgggcggc
    15361 gcgaacgaaa tgctcaacaa actgattttg agccgcgcca acccgattgc cgacgcggtg
    15421 tacggtttgg acaacgccaa tatcggcaag gcgcgggaaa tgggcatttt ggcggcggcg
    15481 caacccgaat ccgcccccgt cgcggtcggg ctgccttcgg ctttggcggt cgattacggc
    15541 tatgtgtcca tcaattacga caaaaaatgg tttgaaggca aaaagctgcc cctgccgcaa
    15601 accctgcagg atttgacccg ccccgaatat aaaaacctat tggtcgtgcc gtcccccgcc
    15661 acgtcgtccc cggggctggg cttcctgatg gcgaacatca gcggtctggg cgaagaaggc
    15721 gcgttcaaat ggtgggcaca gatgcggcag aacggcgtga aggtcgccaa aggctggagc
    15781 gaggcgtatt acaccgactt ttcgcacaac ggcggcgcgt atccgctggt ggtcggttat
    15841 gccgccagcc cggcggcgga agtgtatttt tccaaaggca aatacagcga gccgccgacg
    15901 ggcaacctgt ttttaaaagg cggcgtattc cgccaggtcg aaggcgcggc ggtcttgaag
    15961 ggcgcgaaac agccggaatt ggcggcaaaa ctggtgcaat ggctgcaaag tcgggaagtg
    16021 cagcaggcgg ttccgtccga aatgtgggtt taccccgccg tcaaaaacac gcgcctgccc
    16081 gacgtgttcc gcttcgccca agccccgacg cacaccaccg cccccgcgca gcgcgatatt
    16141 gatgcgaacc agcgcggatg ggtttcccgt tggattagaa cggttttgaa ataaaacaaa
    16201 cataccgccc cgcagggctt catacggcat ttttacacct gtgccgatta cgccgcacgg
    16261 ggcggatgtt cgatcaagag gaaaacaatg gacttcaaac aatttgattt tttacacctg
    16321 atcagtgttt ccggttggga gcatctggct gaaaaggcgt gggcgttcgg gctgaacctt
    16381 gccgccgcgc tgcttatttt tttggtcgga aaatgggcgg cgaaacgcat tgtcgctgtg
    16441 atgagggcgg cgatgacgcg cgcgcaggtc gatgccacgc tgattagttt tttgtgtaat
    16501 gttgccaata tcggcttatt gattttggtg attattgccg cattgggcag attgggcgtt
    16561 tccacaacat ccgtaaccgc cttaatcggc ggcgcgggtt tggcggtggc gttgtccttg
    16621 aaagaccagc tgtccaattt tgccgccggc gcgctgatta tcctgttccg cccgttcaaa
    16681 gtcggcgatt ttatccgcgt cggcggtttt gaaggatatg tccgagagat taaaatggtg
    16741 cagacttctt tgcggacgac cgacaacgaa gaagtcgtgc tgcccaacag cgtggtgatg
    16801 ggcaacagca tcgtcaaccg ttccacactg ccgctgtgcc gcgcccaagt gatagtcggc
    16861 gtcgattaca actgcgattt gaaagtggcg aaagaggcgg tgttgaaagc cgccgtcgaa
    16921 caccccttga gcgttcaaaa cgaagagcgg caggccgccg cctacatcac cgccttgggc
    16981 gacaatgcca tcgaaatcac attatgggct tgggcaaacg aagcagaccg ctggacgctg
    17041 caatgcgact tgaacgaaca agtggtcgaa aacctccgca aagtcaatat caacatcccg
    17101 ttcccgcaac gcgacataca catcatcaat tcttaaacgc cgtctgaaag aggagtggga
    17161 aatggacgcg ctgcacacca tcgcccgaaa cctgacgaaa aaacgtcaaa ccgtaagctg
    17221 tgccgaatcc tgcacgggtg ggctgcttgc cgccgtattc acaagcgttg caggcagttc
    17281 gcaatggttc gatcaaagtt ttgtaacata cagcaacaaa gccaaagaag accgcttggg
    17341 cgtgttgccc gaaaccctgc tcgaacacgg cgcggtcagc cgccaaaccg tctatgagat
    17401 ggcgcgcggc gcgaaagccg tggcgcaggc ggattacgcc gtcggtattt ccggcatcgc
    17461 cggtccgggc ggcggcagcg aaagcaaacc cgtcggcacg gtttggttcg ggtttgcctt
    17521 tccgggcgga agttgcgaag caatgcgccg ttttgacggc aaccgcgaat ccgtccgcgc
    17581 gcaggcggtc gccttcgcgt tggaacggtt ggcggggctg attgaaaacg gcggcgatgc
    17641 cgtctaaaca aaatctccgt ctggacaaaa tccccgccgg ataaaaaata ccgtctgaaa
    17701 cgttgcaggt ttcagacggt attttgtcgg gttaggcggc ggtgcggctt atttcacttt
    17761 actcttcaac gcgccgtagc ctgccgcgtc catttgttcc agcgggatga atttcaagct
    17821 cgcgccgttg atgcagtagc gcagtccgcc tttgtcgcgc gggccgtcgg ggaagacgtg
    17881 tcccaaatgc gaatcggcgg cgtggctgcg cacttcggtg cggcgcatgt tgtagctgaa
    17941 atcatcgtgt tcggtaacgg attttgcatc aatcgggcgc gtgaagctcg gccagccgca
    18001 gccggaatca tatttgtcgg cggagctgaa caaaggttcg ccgctgacaa cgtccacata
    18061 aatgccgggt ttgaacaaat ggtcgtattc gtggctgaag gcatattcgg tcgcgctgtt
    18121 ttgggtaact tggtattgct cttcggtcag ggtgcgtttg agttcggcgt cactcggttt
    18181 tttatacgtt gccgcgtcga agcctttgcc ttgcggtgcg gtcttggttt tgcccggcag
    18241 cggttcgtcg gctttgcgga tatcgatgtg gcagtagccg ttggggtttt taatcaagta
    18301 gtcctgatgg tattcctcgg catcgtagaa gtttttcagc ggctcgtttt caacaacgag
    18361 gggcagttgg tatttttgct gctcgcgttt gagggcggcg gcgatgacgg ctttttcggc
    18421 ggggtcggtg tagtacacgc cgctgcggta ttgcgtaccg gtgtcgttgc cctgtttgtt
    18481 gaggctggtc ggatcaacga cgcggaagaa atattgcagg atgtcgtcta ggctgagttt
    18541 gtcggcatcg taggtcactt taacggtttc ggcgtggccc gtatggcggt aggacacgtc
    18601 ttcatagctc ggatttttcg tgttgccgtt ggcgtagccg gataccgcgt caaccacgcc
    18661 gtcgatgcgt tggaaatagg cttccaagcc ccagaagcag ccgccggcga ggtaaatggt
    18721 gcgcgtgttc atgatttttg aatccttttt ctgagtgtcg ggtttgtaga acgaatgttt
    18781 caaactgccc aaatcggcat tcgggtcgcg gattaacgcc aacgcctgcg cttcgttgat
    18841 gctgcctttg acgatgcgct gcacgtcgcc gtctttaccg attaacgccc acgaagggta
    18901 aacgctgata ttcaggcttt gggcgatcgt gccgccgttg tcggttacga cgggcagctt
    18961 gggataattc aaaccggcat accatttttg gaagtcgccg tcttttttct cgtgcaaaaa
    19021 gcccggagac gcgacggtaa tcaggttggc ggagctgaat tttgcatctt gcgcccattt
    19081 ttcggtctgt cccaattcgg acagacacaa aggacaccag cttgcccaaa atttaatcag
    19141 cgtcggtttg tcttttttca agtaaacact ggcggggcgg ttgtctgcgg ttttcaaagt
    19201 ggataaagtg tgcggcacgg tcgcggctcc ggcatcgacg attttgggcg aacaagcacc
    19261 cagcgcaagc aggcagccga acttggcgca aagggaaaag aaagtacggt gtttcatttt
    19321 aatgtttcct gtgtggacgg tttgcatgat tagacgtttg agatgccgaa accttacagc
    19381 ccggattttc agacaacctt gccgcgcaaa atacgctaca atacacccta tttcaagttt
    19441 ctaaaattaa aaggaaaatt caatgttcag cttcttccgt cgcaagaaaa aacaggaaac
    19501 gccggctccc gaggaggctc aagttcagga aactgcagca aaagcagaat ccgaagttac
    19561 tcaaatagtt gaaaatatta aagaagaagt cgaatcttta acggaaagcg tcaaagggca
    19621 ggtcgaatcg gctgttgaaa ccgtcagcgg tgcggttgaa caggtaaagg aaaccgttgc
    19681 cgagatgctg tctgaagcag aggaagcggc ggaaaaagca gcggaacaag tcgaagcggc
    19741 aaaagaagcc gttgccgaaa ccgtcggcga ggctgtcggg caagttcaag aagccgttgc
    19801 gacaactgaa gaacacaagc tcggttgggc ggcgcgtttg aaacaaggtt tgaccaaatc
    19861 gcgcgacaaa atggcgaaat cgctggcggg cgtgttcggc ggcggacaaa tcgacgaaga
    19921 tttgtacgaa gagctggaaa ccgtgctgat taccagcgat atgggcatgg aagccaccga
    19981 atacctgatg aaagacgtgc gcgaccgcgt cagcctcaaa gggctgaaag acggcaacga
    20041 attgcgcggc gcgctgaaag aagccttgta cgacctgatt aagcctctag agaaaccgct
    20101 ggttttgccc gaaaccaaag agccgtttgt catcatgctt gctggcatca acggcgcggg
    20161 caaaaccaca tccatcggca aactcgccaa atatttccaa gcgcagggca aatctgtgct
    20221 gcttgccgcc ggcgatactt tccgcgccgc cgcgcgtgag cagcttcaag cttggggcga
    20281 acgcaacaac gtaaccgtga tttcgcaaac cacgggcgat tccgccgccg tgtgcttcga
    20341 tgccgtccaa gccgccaaag cgcgcggcat cgacattgtg ctggccgaca ccgccggccg
    20401 cctgcccacg cagcttcact tgatggaaga aatcaaaaaa gtgaaacgcg tgctgcaaaa
    20461 agccatgccc gacgcgccac acgaaatcat cgtcgtgctt gatgccaata tcggtcaaaa
    20521 cgccgtcaac caagtcaaag cctttgacga cgcattgggg ctgaccggtt taattgttac
    20581 caagctagac ggcacggcaa aaggcggcat cctcgccgcg cttgcctccg accgccccgt
    20641 ccccgtccgc tatatcggcg tgggcgaagg catagacgat ttgcgcccgt ttgacgcgcg
    20701 cgcgtttgtg gacgcactgc tggattgagc cgaaatgccg t
//
LOCUS       DAXIHS010000030        20425 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXIHS010000030 DAXIHS010000000
VERSION     DAXIHS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.030"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(430..505)
                     /locus_tag="ACFOF2_000977"
     tRNA            complement(430..505)
                     /locus_tag="ACFOF2_000977"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Asn,seq:gtt)
     gene            complement(598..957)
                     /locus_tag="ACFOF2_000978"
     CDS             complement(598..957)
                     /locus_tag="ACFOF2_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC7107318.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(980..1624)
                     /locus_tag="ACFOF2_000979"
     CDS             complement(980..1624)
                     /locus_tag="ACFOF2_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC7107319.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRA"
     gene            complement(1626..2162)
                     /locus_tag="ACFOF2_000980"
     CDS             complement(1626..2162)
                     /locus_tag="ACFOF2_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC7107320.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2174..3271)
                     /locus_tag="ACFOF2_000981"
     CDS             complement(2174..3271)
                     /locus_tag="ACFOF2_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC7107321.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3274..4710)
                     /locus_tag="ACFOF2_000982"
     CDS             complement(3274..4710)
                     /locus_tag="ACFOF2_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC7107322.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4930..5712)
                     /gene="bioC"
                     /locus_tag="ACFOF2_000983"
     CDS             complement(4930..5712)
                     /gene="bioC"
                     /locus_tag="ACFOF2_000983"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC7107323.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5700..6347)
                     /locus_tag="ACFOF2_000984"
     CDS             complement(5700..6347)
                     /locus_tag="ACFOF2_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC7107324.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6357..7499)
                     /gene="bioF"
                     /locus_tag="ACFOF2_000985"
     CDS             complement(6357..7499)
                     /gene="bioF"
                     /locus_tag="ACFOF2_000985"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC7107325.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            7722..8798
                     /locus_tag="ACFOF2_000986"
     CDS             7722..8798
                     /locus_tag="ACFOF2_000986"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC7107326.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(9005..10471)
                     /locus_tag="ACFOF2_000987"
     CDS             complement(9005..10471)
                     /locus_tag="ACFOF2_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC7107327.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10669..11592)
                     /gene="speB"
                     /locus_tag="ACFOF2_000988"
     CDS             complement(10669..11592)
                     /gene="speB"
                     /locus_tag="ACFOF2_000988"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC7107328.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11691..13583)
                     /gene="speA"
                     /locus_tag="ACFOF2_000989"
     CDS             complement(11691..13583)
                     /gene="speA"
                     /locus_tag="ACFOF2_000989"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC7107329.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13750..14034)
                     /locus_tag="ACFOF2_000990"
     CDS             complement(13750..14034)
                     /locus_tag="ACFOF2_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC7107330.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14185..15993)
                     /gene="aspS"
                     /locus_tag="ACFOF2_000991"
     CDS             complement(14185..15993)
                     /gene="aspS"
                     /locus_tag="ACFOF2_000991"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC7107331.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(16051..16752)
                     /locus_tag="ACFOF2_000992"
     CDS             complement(16051..16752)
                     /locus_tag="ACFOF2_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC7107332.1"
                     /translation="MTEPEAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16895..18043)
                     /locus_tag="ACFOF2_000993"
     CDS             complement(16895..18043)
                     /locus_tag="ACFOF2_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC7107333.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18204..18467)
                     /gene="rpsT"
                     /locus_tag="ACFOF2_000994"
     CDS             complement(18204..18467)
                     /gene="rpsT"
                     /locus_tag="ACFOF2_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC7107334.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18770..19912
                     /locus_tag="ACFOF2_000995"
     CDS             18770..19912
                     /locus_tag="ACFOF2_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC7107335.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5266 a         5253 c         4751 g         5155 t
ORIGIN      
        1 tatttgggta gaattaggga gtgattggta gcggaaatag acgaaaacct gtgtttgggt
       61 ttcggctgtc gggagggaaa ggaattttgc aaaggtctca attagtatag tggattaaca
      121 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggaac cgattcactc
      181 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgagccaa tgccgtactg
      241 gtttttgtta atccactata aaatcgagtt tatcctagct gtccaggaca gcccctattt
      301 tttcataacc atcacgaaag gaattttgca aagattttat tccatctcaa aacaatcatc
      361 tcaaaaatgc gtttctgacc gccggtaaaa caaaaccctc taagaaaata cttagagggt
      421 tttgaaattt ggctccccga cctgggctcg aaccagggac ctgcggatta acagtccgtc
      481 gctctaccga ctgagctatc ggggaacaag cgcgaataat aaaacagaaa taccgaacgt
      541 gtcaatataa attgacaatc gattgcactt tatctgcaag cccgcaatct ttatatgtta
      601 tatggcttcc gcaatatgcc ttgccgcacg tttggcatcc aataggatta aaccaagtct
      661 gccggtttct tttgccacca gcaccaacac ggcatcttta cccgcctgac ttaaaaggat
      721 atagcctgat tttcctttaa tcatcacttg ttccaattcc ccgcaggcga gttcctgcac
      781 cgagcgactc cccaaagcaa gcaaagtggc agaaatcgcc cctaccctgt ccgaattcaa
      841 atgtgaagga agcattgtcg ccatcggcaa tccgtcggtt gagataacgg cagacgcgat
      901 aatatccgta gatgtattgt ttaaatcttc aaggattgaa atcaataatt gctgcataac
      961 ccctcctctc tcccaagttt tacgcgcggt tgctgtaacg gcggtataaa atccttacca
     1021 aagtaacaaa tgcctctttg cccaaatcgg gaatgccgcc gataaccaaa ataaatttgg
     1081 ttgaaccgat atacaatggg aaaaatgtca attcgctctg accggaagga tcgcaaacgc
     1141 cccaagcgtt attgttgata tacaggttgt tcttaatcag cagccggtat ttcttttcca
     1201 tctgtgcgac ttctgccgcc aacaacccca actcttccgc cgcctcatga tggaaattgg
     1261 cgttggcaag atacagaccg ttccgatcga ccaataacgc cttaccgctg ccggacaatt
     1321 gttccatcag caacggcaat tgctcgtccg acaaattgat gccgtctgaa tgaccgtttt
     1381 catcgccata gaggaattcg agtttttgca aacggtacaa caggttcaaa gcggtatcga
     1441 tgtcggcggt gtccgcccaa gtaagcagct tctcactgct gaccatttcg tccgcatctg
     1501 ctttcaacag gctgtgcaac aaagttttac cggcactggg ggcatcgctg gatacggcat
     1561 aaaatgcacc ggcaggagtc aggcggggat ataaatttgc ttgtagtgaa agtgttgatt
     1621 ccatattaaa cctccagtcc cggatcaata gaaaataaca ttgcgctaac caattgtttt
     1681 acgtcatctt ccttacgggc atcaatttca aaaaccggaa cattaagatt atgttttgca
     1741 agatatttgt gatacacgtc gataccgggc tgagagcgta tatccatctt ggtaataccg
     1801 acaacgacgg gtgccttctc cagcagccct cgaaacgaat gtaaaaagaa ttccaaatct
     1861 ttcaacggat tggttcgggc attatctaaa agcaagacca aacccatact gccttggctt
     1921 aagatttccc acataaagtt gaaccgttcc tgaccgggcg taccatataa atggactttg
     1981 gtatcctcat ccaagctgat ggccccgtag tccatcgcca ctgtcgtatt ccttttccta
     2041 tccaaagtca tatcggatgc ggaagcatcg gtctgaacga gtgcttcgtc cgaaatagcc
     2101 gcaatggcag tggttttccc tacgccgaca ggtcctgtga aaataatttt attttctctc
     2161 atctctccgc ctcttagctg cccaacagtt ttttcatcag cctttgcaga aggccgcgcg
     2221 actgggattg ggatggtgtt gtgatttttt ccgctttttt catcatttca ctatcagagg
     2281 cagaatcggc tccgatattt attttatccg ccatatcgga gtatgcctgt tgtgaaaccg
     2341 tttttaaatc taccgacaaa aacccggttg tataggttgc cgcaagataa ttcagaatat
     2401 cattgaggtt taaaggcatc actttataca acacgttaag gttgacggat gccttggtca
     2461 gaaatgccga caagcgtatc gaccccggca catttgccaa ccgggtcagg tttggccaag
     2521 atttcaacgt aaacggagta tcgggagaaa tcggataaat caacctgccc tgcgctgtcc
     2581 aaatggaaaa ctgccacata caggacataa tgcctacttt agccttttcg cgccattgcg
     2641 ggttatcggg aacagtcttg cagctgacct gcaaattttc gtctttgcac aattcttcga
     2701 gtttttgcac actttctgtc agcaaaaccc gttgtatcga ggggaaaaca ataaggaccg
     2761 gcttatttcc atgcaagata gcgatgtcct gcctgttctt ttccgcaaac cgcaacgccc
     2821 ccaataatcc tttattcggg ttaaactggc gtatcgtaac cgtacgctgc acattcccgt
     2881 tatttttcgc cgaccggtct gcgggtgcaa taaacgattt cccataaaca ttctcgccct
     2941 gcaacaattt gcggagcata ggaaacaatg tttcaaaccg aatcggtttg ggcaggtagg
     3001 gaacttcaga atcgggaact ttctccgaac agacggcgac gggtatatcc ttataacgct
     3061 cggcaagctc tttccaaagt tcaaaaccgc cctcggcatc ggtatccgcc aaaaccaaat
     3121 cgggcacggc actgccgtct gaaggggata ctgtttcata acgggtggta ttgtgcattt
     3181 tgaatgccat tttgaaaacg gattcctgct gcgccgtcat ccccgccaac attacgcgta
     3241 ctgttttaat tttcggcagt tgaacttcca tttttatttt ccgtaccgtt ttattttttt
     3301 aatattttga ttcatacgct gcagcagccg gctcatcagc ataacgacct cttcaggaag
     3361 cctgtccgca cgttccctca atacccttaa aaactgcccc aacctatccc aatcttcagt
     3421 acgttcataa atatcgatca acgtaatata aagctgggac tcgtcgggat atttcaatac
     3481 cgcctgctcc aacacatcca ttgccgcttc aatctgacca tacatcagca acgactctac
     3541 ttccttaacc gcatcgtctg ccggagacga accggtgttg atcaacgaag aatcttgaag
     3601 caccaaatcc cgatgttgcg atttgaattt ctgtatattt ttcggcagat acccgtgccc
     3661 cataccgatg tctttgattt gccggtcgtt cggccctttt tccaaatcat cgaaaacctc
     3721 atggtaaccc aggctgtacc cccaccccag catccgctct ttaacctgcc tgccgtagtt
     3781 gcccaacgtt tggtaaagtt tccacaaatg tccggcaaaa cggtctatgt ccgcgtattg
     3841 gtaatcgagt ttcaacgcat cgataatcag gtttgcaggt ttttcggaag tttggatggc
     3901 acggtcgtat tgtttcgatg ccgtttcata gctgactttg tctttaagga ttttcgcacc
     3961 ttgatcggca cggaccaaac cggcaatcgc accgatttcc tcttgactga ttccggacac
     4021 gtcttttttg ccccgcacaa tcgggatgcg cttgatttct tcggcttcat aagccttacc
     4081 gccggcatcc ggcgggggcg atgcggaagc tgccgtcgca gaaccgcctt ccgcacgttt
     4141 ttcaatctcc tgagtacccc atcccaaacc ttcttccgcc aagacgcgga tacgcaaatg
     4201 gtttgaatcg cgctgtaatg cctgttcgat atattttgcc aaaagttcgg aaggaatcag
     4261 tttgccgtat ttttgcagat tgtctgccaa aacatcgaca tcccccactt cgagattgat
     4321 atcgagcagc tcgcggataa ggttttcagg tttcgcttca ccatccggaa tgccgtccag
     4381 ataggcagcc aaagattcgg cagccttgcc ctgataaccg aattgcttat aaacctgata
     4441 ctccgtaagc ggatcgactt cttgcgcgga tacggcggcg gatgcggtct cggcactttc
     4501 gttccaagac cagtcgggtt gcgtgccgtc tgaaaccgat tgtccaatct gatcgaccca
     4561 gtccgaatca ttgcttccgg gcggctgccc ctgcgtattg ccgacacctt gggatttgcg
     4621 cttcggtgtt ttcttaccct gcttcgcacg cataaccaaa agcaccagca ataccgccag
     4681 tgccagaccg ataattaatg agttttccaa aggatactcc cgataccgta acgagcggat
     4741 agctgaccgc ctgcaacggc cattgtccgc ttttattatt aacgatttac tgaaatagac
     4801 ttgtaagttt taaatcatac cataatttaa cgtttaacaa tatgccttct gcacaagcct
     4861 cgccatatta ccttttaccc acatcagtat caatacccga tataaaaata actttgccca
     4921 taagctgcct tattgcctgc ccgccgcaga gtagcgcgcg ataaaaaata acggatgata
     4981 agtcaggcgc actttgcccg acggcatact gaacgccgac aagtaatcgc caataaatcc
     5041 attgagattt tttcttgtcc aatttttttg gttcgtggct gttacgcctg tatatttaag
     5101 gtgtttgagt acatctaacg gcgtatcaaa gtctagtatt accttaaaat cctcacacca
     5161 taaaagctca aaatctttgg ctaaccaagt ctgccattgg gataaagtcg ggtaatttaa
     5221 gcctatattt gtaatttggc ggacttcttt taaattgtct ttgccaaagg ttgcaaccgc
     5281 caataatccg tttgctttca agcctgtttt gcaatgggcg ataaaagcgt cgggttgatg
     5341 aaaccattgc acggcagatg cgcttgcgat taagtcaaat tgtcgttgaa aaggaaagtt
     5401 ttccgcatcg ccgcaataaa aatcaaagga ttgcggcagt ttttcagcca gttggggctg
     5461 cacatcgcac aaatcattaa ataaccaata attcgctgaa atctgttttt gcagcaaggc
     5521 actcaacatt cctgagccgc agcccaattc caacacgttt tccaatggca tatccggcaa
     5581 ataatcttgc aaatgcgcca ttagattaat cgtcattttt tgttggatta aggcgtgccg
     5641 gtcataatcg tttaatgctt tttggaaagc ctgccgaatg cgcgatttat ttatggcagt
     5701 cagtgattcc atagtgccga ccaatgggtg aatcttgaaa acaggtaatg tccgccgtca
     5761 atttcccgaa cggtgcaacg cggtgtccaa tatcggtgct gattggcagg cataaaaatt
     5821 ttatcgcccg atccgaccaa ggcatttgtc cagcgaatca aatctgcgcg cctatcttgc
     5881 ccgatcatcg caaaaagtgc gataagttct tgatgaattt cgtcaaacgg acgggcggga
     5941 aattgttggt aatcttcaaa agatgcttta tcgccacaca ttctgcgttc aaattttaaa
     6001 cgggtgtttt ccgtgaggtt ctccaatgtg cctttaaaaa cggcgcaagg gataccgaaa
     6061 ttatcatcgc aaggcaaacc tgtgccattc actgccgttg cggattttaa tcttattcct
     6121 tgcaatgccc tctctgccgc ccaaacgccc attgaccacg ccaccaaacg gatgtgccga
     6181 taggcggaaa aatcaaaatc caaatttaaa tcttgataat catagcaaat caataaatcg
     6241 tgattttccg gcaaaatcaa atgatttaca gcatcgggcg gcgttcccca acctgcaaaa
     6301 tacaggatta aatgtccgcc ttgatgattg taaaattttg ttttcatatc atatccttac
     6361 aggcacaccg caaactgccg cacttcatcc gttgtcatat ctgccgttaa agacaggcgg
     6421 attctggatg tgtttttggg tactgtcggt ggtctgatgg gcaggcaata ataaccctgc
     6481 ctttgcaggt attccgcttt ggcaagggtg gcttcattcc cgcctaaaat ataggggacg
     6541 atacaggttt ggctcggcat tatttgcgtc cgatgcgcca cttcccgccg taaaaatgcg
     6601 cttaactgct caagatggct tctttctttt gagaattgcg gcaatcgttc aaaaataaaa
     6661 taagtccaag ccacattaaa cggcggcaat gcggttgaaa aaatcaatgg gcgcatttga
     6721 ttaatcaaac attctttcaa tacttggttg cagacggcat acgcccccac cgaggctaag
     6781 gctttaccga aagtgccgac caataaatca atctcggcaa tcaaattatc ccgttcggca
     6841 atccccaatc cgttttgccc ataaacaccg attgcgtggg cttcatccac ataaagataa
     6901 gtattgggaa actgtttttt taattggaca agctgtttca aatccgccac atcgccgtcc
     6961 atactgaaaa cagattcggt aacgataaaa gtgcggtcaa attttccgac gtttttttca
     7021 agcagatttt tcaaatgttc ataatcatta tgacgataac gggaaaacgc acaccggctc
     7081 aaacggatgc cgtcaatcat actggcgtga acaaatttat ctgccaaaat caaacttttc
     7141 gtcgtcgtca aagcaggcaa aataccgaga ttggcgtgat agccgctgtt gaacaataac
     7201 gcgctttccc gttggaaacg ttgtgcgaca agctcttcca aatcggtata aataggaaag
     7261 ttgcccgtta ataaacgcga tgaagaactg gtaaaagagg gaaaattacc gccgtattgc
     7321 tgcaaaaaag accggcgcaa gttttcatct gatgccaaac ccaaataatc attagacgac
     7381 atattcagca ttttgcagtt ttcccgcgta atataccgcc cttgatgaat caaatccgga
     7441 atcgaacgat attggttttg cgcgccgagt tgttcaagct gttgtttaaa aaccttcatt
     7501 atgatgtaaa tattcctgag ttaaagcctg aaccaaccca gcatcaaaca atcaagtaat
     7561 gcccttttaa taaaaaccgt tttgccgccg acctttgcca cacccctgcc ttcataatcc
     7621 aaggcggaaa tttccgcgac atttgtttcc gttgccatta cccaatccgt ccgcactgta
     7681 ccgaagcgca tattttcgca caaattggta cggtttcagg tatgatttgc cgaaaaccta
     7741 tcggacacac accatatttt gatatgaata aaacaatctg ccgcactgcc gcactattga
     7801 taagcggttt ttcatacgca aacaccgtca ttcccgatgt ctcccccgtc gcacaaggtc
     7861 aacatgtctt cattaacatc cctcagcaac gcctgttcct ctacaccgac ggcaaactga
     7921 ccaaggttta tcccgttgca gtaggtcggg cgatgacgca aaccaatcta ggcgaacata
     7981 aaatcggtgc gaaagcctat aaccctgttt ggtacatccc caaatccata cagaaagaac
     8041 gcggcgacgg cgtaaaaacc atagccgccg gcccggacaa ccctttgggg ccggtttttg
     8101 tccgcttagg cgaccccaaa ctcggtttgg gcatacacgg gaccaatgcg ccggccagcg
     8161 tccccggggt tcggagtcac ggctgcgtcc gcatgaaatc gcccgacgcg ctcgagtttg
     8221 ccaaaaccat cgccagcggc tcgcccgcct ccgtcatcta tcaaatggcg gggctcaatg
     8281 aagatgcgga tcgcaacctg tggcttgccg ccttccgcga cccttacggt aagaacaacc
     8341 ttgacatcgc ctctctgaaa aaaagtattg cgcaatgggc aaaaacacag ggtaaaacca
     8401 tcgcgcccga gaaagtcgat gctgtactca aagaccgcac cggatcggcc gtctgcctga
     8461 cctgcggcaa aaacggcaag atgaagatgc cgctcaaatc gctggcgtgg atacagggtt
     8521 cttcctcata cagccaagct gaagtcattg aacaaactga ggaaaccaac tctgccgaaa
     8581 tatccgagac acgcacgccc gaagtgcctg atgtacacac gcccgaggca caaccgcatt
     8641 taaataccca atccgacggc acgccgactg cctataccga accggctgcc gattcatcgc
     8701 cgcaagtaga aacacctgat caggctgctt ccgagccggt tgatgtatta ttttcaatag
     8761 atgtgatacg gcagggaaat ttgcgtttag gtaattgaat aaccttctga ttatttaatg
     8821 cttatgttta tcagaagttg ataggcggtt tggtttttgt ttggcttttc ctacaccgcc
     8881 gcctgccgct ttctgcaaca ttcaagcgca caaatatgcc atctgaaggc tttagacggc
     8941 atatttcacg ataatagtcg cccaatcaaa taaacacaga ttggaaattt attttatttt
     9001 tcccttacca ataccccaga actttccacc aaatgctgcc gataacgaaa aaaatcagaa
     9061 aattgaccac gctcatgata aaacccgcct tccaccattc tcccattgtg gtgtagcccg
     9121 aaccgaaaat cacaggcgaa gtaccggtcg cataatgagt gagggtcatc ataatgttgg
     9181 atgcggccgc catcatcagc gcggtcggca tcgccggggc attcagtgaa acggcagcag
     9241 cgaaaaatgc gccgaacata gcggtaatat gtgcagtagt actggcaaac atataatgcg
     9301 catacatata agcaagcacg aggattacgc ccgcagccgt gccgctaacg cccaaaccgc
     9361 cgacactttc cgccaacact ccggagaacc atttaatcag tccgagttta tttaaaaatg
     9421 cggccatcat aatcaatgcg ccaaaccaaa taatcgtatc ccacgcgctt ttttctttca
     9481 aaacatcgtc ccaagtcaat acaccggaaa gcaaaagcag gcttaatccg ataaatgcgg
     9541 tggcggtggc gttgatacta aaagcgtgat tgccggtaat aagggcggga acatctgccc
     9601 acaacagcaa caagataccg aaaatgaccg ccataatgat ttcgtctgcc gacattttac
     9661 ccatctccct cagacggtct ttggcaaatt gaacggcatt gggcgtttct ttaatttcag
     9721 gcggatacaa aaaatataaa atcaaaggca taacgaaaaa ggcgataacg ccgggaacag
     9781 ccattgccca cgcccacgcc ccccaagaaa gacggaaact actgcctaaa ttttcggcaa
     9841 tcaagttgac gattaaaggg ttgggggcag ttgcagtaat aaacatagcc gacgaaatgg
     9901 gattggaatg atagttgacc aaagccaaat atttacccat cttgccttct gtgccttttg
     9961 cgggattgga gccgtaactg ccggcaatcg actgcataat cggatgtata atgccgccgc
    10021 cgcgcgcggt attggaaggg gtaacgggag ccagcagcag ttcggaaaga gcgagactgt
    10081 aaccgatgcc cagcgttttt cttccaaaaa cggcgataaa caaatatccg atacgcatcc
    10141 ccagccctgt tttgagcaaa ccgcgcgaaa tcataactgc gatggcaatc agccaaatca
    10201 acggattggc gaacgcactc aacgcatcgc tcatcgccgc acccggtttg tcggcggtta
    10261 cgccggttac tgcgaccaac ccgacggcaa taatcgacag cgcacccaac ggcatggcct
    10321 tgccgataat ggcggcaatc acaccgataa acatggccag cagcgtccaa gcctgaggct
    10381 tgaccccgtc gggtacgggc agtgccaaaa ccaaggcgca caatactgcg gcaatggcga
    10441 ggggtatcgg tttgaaaccc aatttcatca tattgacctc cgtaaaaaag accgtcccga
    10501 aaaatcggaa aaataatatt taactaattg ttttataaga tatattccga tatttcaccg
    10561 tctttccgat atgcggctcc gggcaacttt tgtttcagta tttgaatttt cattagactg
    10621 aatacgccgt ttgaacggca cggcgaaaac ccgggggatg ccggacgttc agtccttttt
    10681 cgcaccttga aggtaaagca tttccaaggc aattgtggca ccggccaaag cggtaatgtc
    10741 ggattggtcg taagaggggg caacttctac aacatccata ccgacgatgt cgagatccgt
    10801 cagcccacgt aggattttta atgccctgtc gctgctcaag ccgccgcata cgggcgtacc
    10861 ggtcccaggg gcgaacgacg ggtccaggca gtcgatgtcg aaagtcaggt aaacgggcat
    10921 attgccgacg gtttctttga ttttacggac ggtctcttca acactgtctt cattgacttg
    10981 gggggcggtc aacacagtaa aaggcaattt tttactgtgt tcggtacgta tgccgatttg
    11041 cacggaatgt gccggatcga tgatgccttc cttgggggcg gtataaaaca tcgtaccgtg
    11101 gtcgtattcg ctgccgttgt cgtaggtgtc ggtgtgcgcg tcaaaatgaa tcagtgcgag
    11161 tttgccgaaa tagcgggcgt gggcgcgcaa caacggtagg gtaatgaaat ggtcgccgcc
    11221 caaactcaaa cagcgtttgc cggaagaaag taatttgccg gcgtgcgctt ccattttttc
    11281 gacaaaatcc ctgctgtcgc caaaagaaaa aaccaagtcg ccgcaatcaa taatgttcag
    11341 gcgttcgcgc acatcaaatg tccatggaaa cctgcggtgc tcccaagcga ggttgacgga
    11401 ggcgcgccgg atggcttcag gaccgaaacg cgcgccggaa cgccctgaaa ccgccatatc
    11461 ataaggcacg ccggtaataa cccaatcggc atgactttca tacggcataa aattaagcgg
    11521 caggcgcaaa aacccgaaat tattggaaac gagggagttg tcggtttgtc ctgccagtgt
    11581 gctgtattgc atcgtaatga ttccttgtaa ttggtttcaa tcggtcgtga tgattggtgt
    11641 ttgagtaaga aaatcgggct tcagacgaca tatccgatgc cttgatgcgt ctattcgtct
    11701 tccaaatagg tataaccatt aagccccgct tcgagttctt ttaagaaaga catagcctgc
    11761 gaggcaggaa ggtctgaatg ttcgatttgt tcgcgatagc gtttcatcag ctctttcgga
    11821 tcttgataaa cgtattcgag catatcggca acggtgtttc cttcatcgta atcgatgacg
    11881 gtaaattgtc cgtcttcccc tacaacaaca tcggcagtgg cagtgtcgcc gaaaagattg
    11941 tgcatattgc cgagtatttc ctgatatgct cccaccataa aaaagcctaa aagcggcggc
    12001 tcttcttcgg gataatcagg cataggcatc gtaccggcga tgccgtcgcc gtcgatgtag
    12061 tggtcaatcg taccgtctga atcgcaggta atgtccaaca acacggcgcg gcgcgcaatc
    12121 ggttcattca aaccggtaat gggacaaaca gggaaaagtt gatctatgcc ccaagcatcg
    12181 ggcaaagatt ggaagagtga gaaattgaca tacagcttat cggcaaaacg ttcttgcaat
    12241 tcgtcaataa tggttcggtg agaccggtgt ttttcattaa acaattcgcc gacttcatga
    12301 cagatattta aatacagttg ctccgcccac gcacgttgcg ccaaactcaa cagcccgaca
    12361 ttatactgat tatgcacatc ggcaagatca aactgccctt cgtgtatcca gctgcgtaag
    12421 gaacgttttt cccgcgaggc ggaaatatcc gtccaagttt cccacatact gtgcaacaca
    12481 cgcggtgctt cgggcgatgg cgcatccagc cggcgcggtt tgtaacgttc aacgcctata
    12541 acattagcaa ccaaaacggc gtgatgtgcg gtaatgccgc gcccgctctc ggtaatgatt
    12601 gtcggatgcg gcagcccgtg ttcgagacaa gcctggctga tgccccatac gactgtggcg
    12661 gcatattcgt tgaggctgta attaacggaa caatccgatt gtgtgcggtt tccttcgtaa
    12721 tccacgccaa gcccgccgcc tacatcaaaa cagcggatat ttacccccag tttgtgcaac
    12781 tcaacataaa accgagccga ttcgtgtaca cctgtggcaa catcacggat gttcccaagc
    12841 tgcgagccca aatggaaatg caaaagctgc aggcaatcca gcctgttttt ttgtttcaaa
    12901 atatcgacca gttgcaaaac ttgggaagcc gacaagccga attttgattt ttccccaccc
    12961 gaagactgcc attttcccga accttgggaa gccagtctgg cgcgcacacc caaacggggc
    13021 ttgatgccga gtttttccgc ctcttccaat accatttgta tttcggacag cttctcaatc
    13081 accaaataaa cctgatgccc cagtttttcg cccatcaagg cgaaacggat atattcacgg
    13141 tctttatagc cgttgcagac gattaatgtt tgccgggtgc cggcgtgtgc caaaaccgcc
    13201 atcagttcgg ctttagaacc agcttccaaa ccatgcggtt gtccgcttga cataagcgat
    13261 tcgatgacgc ggcggtgttg gttgaccttg atagggtaaa ccaaacaata accgccctta
    13321 tagccgcact cttcccgtgc cgtctgaaag gcgcggttga tgtcgcggag gcggtgttcg
    13381 aggatttgcg gaaaacaaaa caaaacaggc aggcgcgcct gatgtttttg ttgcacggct
    13441 tcagtcagtt tttgcagtga aacagtttga ttgtgttgcg agggattggg gcggacgatg
    13501 atttcgccgg aatcgtcaac atcataataa cctatacccc aatgattaat gttgcacact
    13561 tcacggatgg taaggatagg cataataaac ctgctccgtc tgtcgtgttg aaaaggaatg
    13621 attataacaa atcagcgtga aatgtcattt ttttaataag aaaagcctgc ctcatacctg
    13681 atgaggaaca ggcaaaatgc cgtctgaacg cttcagacgg cattttggtt cagcttgcca
    13741 tcaaaggagt cagcgatcga gctgctcttt gatgattttc aggtcggggt aagacaacac
    13801 gatgtcgtat tcgcggccgt ctttataggc ttccacttcc agcacaggct taccccaatg
    13861 gtcgtcggca tcgacatcgt gaacctgata accacgctgt tccaacattt tcacggcttt
    13921 tgtgcggttt tgttcaaaat ggggatcgcc gtaaatctga cgctcggcag aatcgccggc
    13981 aaatgcggtt gcggcactca gggaaacaac ggcggccaat aacagttttt tcattttcag
    14041 tccttttttt atcggttgat tgaacaagat gtgtttttca ataccgccat taaaacacag
    14101 caaaattagg tttgaattag agggaagtca agaagcgtaa atgccgtctg aaaaaaacag
    14161 agccgtcaaa cggctctgtt ttccttatgc ttctttggtt tcagccgcct tttgacgcag
    14221 gcgcaagctc aattcgcgca actgcttgtc gtccacgctg ttgggcgcgt tggtcagcag
    14281 gcattgagca cgttgtgttt tcgggaaggc aatcacgtcg cggatggatt cggctccggt
    14341 catcagcgtt accagacggt cgaggccgaa tgcaaggccg ccgtgaggag gcgcaccgaa
    14401 tttcaggttg tccaagagga agccgaattt ctcttgttgc tcttcagggc tgattttcag
    14461 cgcggcaaac actttctctt gcacgtctgc gcggtggata cggatagagc cgccgccgat
    14521 ttcccagccg ttcaatacca tatcgtaggc gcgtgccaaa caatttgccg ggtcggaaac
    14581 catcaggtct tcatgacctt cttttggcgc agtaaacgga tggtgtacgg caacgtagcg
    14641 gtcggcttct tcgtcgtatt cgaacattgg gaagtcgaca acccacaaag gtttccattc
    14701 gtctgtgaaa tagccgtttt ccgcgccgtg ttccaagccg actttgatac gcaatgcgcc
    14761 gatggcttcg tttacgactt tggttttgtc tgcaccgaag aagataatat cgccgttttg
    14821 tgcgccggta cgcgcgatga tttccttcag ggcattctca gacaggaatt ttacgattgg
    14881 agattgcagg ccgctgtctt cgccgttgga gaggttgccc acatcattta ctttgatgta
    14941 tgccagacct ttcgcaccgt agatgccgac aaatttggtg tattcgtcga tttctttgcg
    15001 gctgaatttc gcgccgttcg gtacacgcag agcgactacg cggccgcctt tcatatctgc
    15061 cgcgccacgg aagactttga attcttccgt tttcatcagg tcggtcaact cggtgaattt
    15121 caagttgata cgcatgtccg gtttgtcaga gccgtagtag aacatggctt cagagtaagg
    15181 catgcgtggg aagtcgccca agtccacgcc cagcgcgtct ttgaagactt gtttcgccat
    15241 accttcggtg atgtccatga tttcatcctc gtttaagaac gaggtttcca agtcgatttg
    15301 ggtaaattca ggctgacggt cggcacgcag gtcttcgtcg cggaagcact tggtgatttg
    15361 gtagtaacgg tcgaaacccg ccaccatcaa cagttgtttg aataattgcg gcgattgcgg
    15421 tagcgcgaaa aactcgcccg gatgaacgcg gctcggcacg aggtagtcgc gcgcgccttc
    15481 aggcgtggag cgggtcagca tcggggtttc aatgtcgatg aaaccttgcg cgtccaagta
    15541 gcggcgaacg cccatagcca cttggtaacg caggcgcagg ttgcgctgca tcaccggacg
    15601 gcgcaggtcg ataacgcggt tggtcaggcg aacgttttcg ctgatgtttt catcgtcgat
    15661 ttggaacgga ggcgtagcgg cggcgtttaa tacttcgatt tctttggcaa gaatttcgat
    15721 tttgccggaa atcattttgt cgttggttgt accttcggga cggttgcgta cgcggccggt
    15781 aatgctcaaa acgtattcgt tgcgggcgga atcggcagca gcaaacgctt ccggagtgtc
    15841 ggggtcaatc acgacttgga cgatgccttc gcggtcgcgc aggtcgataa aaatcacacc
    15901 gccgtggtcg cgtcggcggt gtacccagcc tttgacggtt acggtttggt ctaagtattg
    15961 ctcgctgatc aggccgcaat agttggtacg cataaattca ccttttattg atttaaactg
    16021 aaaacagaaa atgccgtctg aacggcggct ttattgttgt tcgggcaaat ccgccttttc
    16081 agacggcata ggtcctgcca atgttttgac gggcaggtcg tcagggatga ccatacccag
    16141 cgaaatcaca tatttcaatg cttcgtccac gctcatatcg agttcgcgca catcgctttt
    16201 ctttaccata atatagtaac cgccggtcgg attcggcgtg gtcggaacat acacggaaag
    16261 ataatcgccg tccttcggca atgcggcctt aaccgcattc gacacctgac cggacacgaa
    16321 tgcgattgtc caaatacccg attggggaaa cggcacgagt actggtgttt taaacgaacg
    16381 gctgctgtcg gacagcagcg attcggatac ttttttcaca ctcgaataga tggacttcac
    16441 aactggaatc cgccccaaca ggctgtccca cgcggcaaga atctgccggc ccaatacgtt
    16501 tgcggcaaac aatccggtta caaacaatac agcaatggca acgataacgc ccagccccgg
    16561 gatattgaag cccaaaacat attgcggccg ccattgcttc ggcagcaggt tgacgagctg
    16621 atcggacgcg gaaacgatat aggaaaccac ccaaaccgtt accgcaatcg gcagccagac
    16681 caaaatgccc gtaatcagat atttttttaa cgccttggca gctttaccgc cttcggcctc
    16741 aggttccgtc atcttgcttg attccgacaa agtccgtaca aaccgcacat tatacgcgtt
    16801 tgcccggatt caaacgaaat ttttatcccg ccccgccaaa ccgccagcgg cttcagacgg
    16861 cacggcaact tgatataccg tctgaacacg cggttcagat gccgtccaag tcgttgaaca
    16921 tcaacccgat accgataccg ttctgcttgt ggttgtagtc gatcaggctc tcgccgtaac
    16981 cgtggaatcc gcgtaccacg cctttgagtt tgcccttaat cggaaacgtg taggcggctt
    17041 caatcgcgcc gtagcccgtt ttggggttgt agcgcaatac ggaatacaca ttctgcctgt
    17101 cgttcaggcg gtactgcagc ttcacgtcgc cataccccat atagtcggca atatcgggat
    17161 tgtcgttttt atcgccgctc tgatcgaacg cacgcaccca cacgcgcgga atcaccgtca
    17221 atttgcccca ttccatgcct gccatggcgt aaatcctgtt ccacgaacgc gattcgggac
    17281 ggctctgtcc gttggactgg tggacaaaac ccgcaccgag catacgcagc ctgccgccga
    17341 acggcaaatc cgccttcaca ggctgggtca ggaaaatttc aggtttgtaa tccgtattgc
    17401 ggaacggcgc ggatttcctg ccttggttgt aaatctgcca atcggatctt tgggtgtagc
    17461 cgaaccacag atccgcgcgg gttttaaaca aatcttcggc aattttgctt ttgaacgaaa
    17521 cctgcaattt ggtttccgca cgtttctgct gtccgaattt ttcctgtaca gtcgtaccgc
    17581 gcgtcggcga acccggggca tagttgggcg aattgttgta ccagagcggc ataaggtaca
    17641 tcggattgtg ttcgcgtacg cccaacagcc cgcgcaaatc gtttttgtcc aagtcgtaca
    17701 tcaggctcaa aggcgtatag atgtcggcgg tttcgcccgc actgtcggca ggaagcgcat
    17761 ccccgccttt ttcaacaaca atgaccgcct cgcccttatc caggctgctg cggacggttt
    17821 ccgtcagatt gagtacggct ttcgactcct gcccttcctg ccctgccgaa gacggaagct
    17881 gtgccgcaaa aatcctgtcg taacacgcca aacgcgtaac attgtccgtc aaagcggcgc
    17941 attgcagcgc ggtctctcca aaagcggatg ccatcggcaa cagtcctgtc aaaagaatat
    18001 agcgcatatt ccgtgtattc atctccgccc ccattgtcgg catattggtt ttcaaacggc
    18061 attttatagc ggattcggat aaaaaaccgc accctttccg ccatttcggg attttgcccc
    18121 cgcaatacag aaaaccccga aaccgtcagg cttcagggtt ttccgcttat cgcgtatcaa
    18181 ccgccttggc ggttttgcaa aaatcaagcc agtgctttta cttttgcaga cagacggctt
    18241 ttgtggcgtg ccgctttgtt tttgtggaac acgcccttgt cggcgatgcg gtcgatgact
    18301 ttgacagact cttggtaaac cgcttgtgcg gcagctttat cgcctgcttc gactgctttc
    18361 aatacttttt tcactgcagt gcggaatgcg gtacgcaggc tagcgttgtg ggcgcgttgt
    18421 ttgacggact ggcgggcacg tttgcgtgct tgtgcgctgt ttgccatatt ggaatctcct
    18481 ggaagaaaaa taaattctgt aaacgcgcaa ttttaaaggg ggtttacatt atatgcaagc
    18541 cttttctctc tatccgcccg tcggacaccc tgacagcgca aaaaaacggc aaaatcgtgc
    18601 ggacgttttc cccctctttt gaaaacaagg taatttttta ggaaaatcag gtcaatatcg
    18661 ctttgagttt gcacaaattt actttacaat gcccaaacct tgcgccgggg tgtttatggg
    18721 gcgtggggtt ttcttttttt ccaacctctt tcatcaggaa tctgcccgta tgaaaaaatc
    18781 cgtattagcc gttttggccg cgctgtctct ggcagcctgc ggcggcagcg aaaaaaacgc
    18841 cgtgcaacct caagcagacg ctgcgtctgc tgccaatgcc gaagccgctg ccaccgatac
    18901 tctgaacatc tacaactggt cgaactatgt tgacgagagt acggtcgaag actttaaaaa
    18961 agccaacaat ctgaagctga cttacgactt gtacgaaaac aatgaaacgc tggaagccaa
    19021 aatgctgacc ggcaaatccg gttacgattt ggttgtgccg ggcatcgcct tcctgccgcg
    19081 ccaaatcgag gcgggcgcgt atcaaaaagt caacaaagac ctgattccca actataaaaa
    19141 catcgatccc gaactcttga aaatgctgga aaccgccgac ccgggcaacc agtatgccgt
    19201 cccctatttc tccggcgtga acacgattgc gattacggcg aaaggcaaag agcttttggg
    19261 cggcaagctg cccgaaaacg gctgggattt gctgttcaaa cccgaataca cccacaagct
    19321 gaaatcctgc ggcatcgccc tgtgggacac cccaagcgaa atgttcccga ttttgctgaa
    19381 ctacttgggc aaagacccca aaggctcgaa tcctgaagat ttgaaggcag cggcggaagt
    19441 gttgaagtct atccgtccgg atgtcaaacg tttcagcccg tccatcattg acgaattggc
    19501 gcgcggcgat atctgcttgg cggcaggtaa cggcggcgat ttgaacttgg cgaaagcacg
    19561 ttccgaagaa gtgaaaaaca acgtcggcat cgaagtgctg aatccgaaag gcatgggctt
    19621 ctggattgag tcttggctga ttcccgccga tgcgaaaaac gtcgccaatg cccacaaata
    19681 catcaactac acgctcgacc ccgaaatcgc ggcgaaaaac ggcatcgccg taaccttcgc
    19741 ccccgccagc aaaccggcgc gcgaaaaaat gcctgccgaa ctggtaaaca cccgttccat
    19801 cttcccgaac gagcaggata tgaaagacgg tttcgtgatg ccgcaaatga gcacggatgc
    19861 gaaaaaactg tcggtcagcc tgtggcagaa aatcaaagtc ggcaccaact gatttgaagc
    19921 attaaaaatg ccgtccgaac atcgttcgga cggcatttta tattggattg aaatagaaat
    19981 atttatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
    20041 agtacgaaac cgattcactt ggtgcttgag caccttagag aatcgttctc tttgagctaa
    20101 ggcgaggcaa cgccgtactg gtttttgtta atccactata ccgtcgttcc cacggtcagg
    20161 atttcagatt gcggacatct gtcagaaaag acaaaaaacc ttccgccgtt attccctaca
    20221 ggcgagaatc cgatccgttg aaattcggtt gttttaaata aattcttgca gctttgattt
    20281 tctgtttttc cgataacgcc gtaactttga aacgcgaaag cggtaatccg atccgttggg
    20341 attttgcaac ttcaaatcaa tccgcaaact gaaatcccgt cattcccgcg cagtcgtgaa
    20401 tccgaacgcg tccgcacgaa aacct
//
LOCUS       DAXIHS010000031        20316 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXIHS010000031 DAXIHS010000000
VERSION     DAXIHS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.031"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(13..819)
                     /locus_tag="ACFOF2_000996"
     CDS             complement(13..819)
                     /locus_tag="ACFOF2_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC7107336.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="ACFOF2_000997"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="ACFOF2_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC7107337.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKTLTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            complement(2741..3436)
                     /locus_tag="ACFOF2_000998"
     CDS             complement(2741..3436)
                     /locus_tag="ACFOF2_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC7107338.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3607..4149)
                     /locus_tag="ACFOF2_000999"
     CDS             complement(3607..4149)
                     /locus_tag="ACFOF2_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC7107339.1"
                     /translation="MLHLKDKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFS
                     KTPEAIFRCNLHTELKKKRKLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVV
                     LDVKQKGVDMRIGLDISSITLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWN
                     HVPPDLMEHIDGLKTKCPKP"
     gene            complement(4655..6766)
                     /locus_tag="ACFOF2_001000"
     CDS             complement(4655..6766)
                     /locus_tag="ACFOF2_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC7107340.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHPNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYGGSENGSLIKRNY
                     AWQQTDNKTLSSNFTLNGDYNIGRFENHLTVGLDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPVLTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNVTPAHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDAQNDPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLDNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYDSRNKEVTTLPGFVRTDAMLGWNRKNLNLTFAVGNLFNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            complement(6952..7557)
                     /locus_tag="ACFOF2_001001"
     CDS             complement(6952..7557)
                     /locus_tag="ACFOF2_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC7107341.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7606..8004)
                     /locus_tag="ACFOF2_001002"
     CDS             complement(7606..8004)
                     /locus_tag="ACFOF2_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC7107342.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            8112..9023
                     /locus_tag="ACFOF2_001003"
     CDS             8112..9023
                     /locus_tag="ACFOF2_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC7107343.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9013..10314
                     /locus_tag="ACFOF2_001004"
     CDS             9013..10314
                     /locus_tag="ACFOF2_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC7107344.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPFYTAASPAGLVRLAVRLNTRAV
                     IADESHTFADKLTDNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAYADFDRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLSAPQPAVLSDGIFLPRNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKNTSLMGAYLSAGCISPRLLARESLERRLNAWVDNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAATNQGNWRLAASRHTYPDIAAAAHKTDPDGT
                     FVRRHIPELAHLPAAVIHTPWRAAGSIDTHGYPAYPVAGIP"
     gene            complement(10676..10783)
                     /locus_tag="ACFOF2_001005"
                     /pseudo
     CDS             complement(10676..10783)
                     /locus_tag="ACFOF2_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10813..12963
                     /gene="dinG"
                     /locus_tag="ACFOF2_001006"
     CDS             10813..12963
                     /gene="dinG"
                     /locus_tag="ACFOF2_001006"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC7107345.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            12999..13613
                     /locus_tag="ACFOF2_001007"
     CDS             12999..13613
                     /locus_tag="ACFOF2_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC7107346.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGQATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(13904..15772)
                     /locus_tag="ACFOF2_001008"
     CDS             complement(13904..15772)
                     /locus_tag="ACFOF2_001008"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC7107347.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
     gene            complement(15853..17223)
                     /gene="purB"
                     /locus_tag="ACFOF2_001009"
     CDS             complement(15853..17223)
                     /gene="purB"
                     /locus_tag="ACFOF2_001009"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC7107348.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(17295..17927)
                     /locus_tag="ACFOF2_001010"
     CDS             complement(17295..17927)
                     /locus_tag="ACFOF2_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC7107349.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            18236..20116
                     /locus_tag="ACFOF2_001011"
     CDS             18236..20116
                     /locus_tag="ACFOF2_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC7107350.1"
                     /translation="MNIFYEESGQFKIAAIVQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSG
                     FNAGEPTCKIEAVNIAKNLRIQAIEPHFNAETGLDEAGEMMFVHHQDLIWFHQFAVAL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT         5020 a         5580 c         5193 g         4523 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcatcagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaaa ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattctga tttatagtgg attaacaaaa atcaggacaa ggcggcgagc cgcagacagt
     2521 acagatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     2581 agctaaggcg agccaacgcc gtactggttt ttgttaatcc gctatatatt ctgatttaaa
     2641 accataaggc tttaagcaat catctcttct ataaagccta aatacaaaag gccgtctgaa
     2701 atcctatttt cagatggcct ttactcattc aatcctcaac ttattgcggc tttttctgct
     2761 cttcgcgtac tttatccacc aactggtcaa tcgtggtcat accggactgc cagtctttaa
     2821 attcaacttg gtatttgccg ccgacaatca cagtcggtgt gccgctgatt tggaatttat
     2881 tggtcaactc ttccatttga gccgcacgcg cttggctttc aggagcctca aatgcagcca
     2941 atactttttt gccgtcaaac gctgtttgct cggacagcca ttttttcagg gtatcggtat
     3001 cggccagatt gattttttga ttaaccatcg catcgaaaat atggctgttg gctttatctg
     3061 attcaccggc catttccact gcggccgcca aacgtgccaa aggtttcatt tcatcacccc
     3121 acacgacatg ctcccggcgc atataggtat cgtctttaaa cgttttgatg tgctcgctca
     3181 agaccggctc aagatgggcg caatgcgggc agaagtagcc gaaaaattcc aatacttcga
     3241 ttttaccggc ctgctgttgc ggaataggcg tagacaatac agtgtagttc acaccttcgt
     3301 tcaactcaac aggggctgcc ggagcagatg agctgctttg gacgctgtct gccggaacgc
     3361 tggtttcagc ctgtttgcta caagcggcca atgccaacag ggtcaatgaa gtcaaagcta
     3421 aggttttcag tttcataggt atctcttgtg tgtcagatta atgtgcggat tttatggcat
     3481 tttattgaag gcgtgttcga tttgagtgaa aaaatgtgtt tagttttatt tcagccttta
     3541 taaaggccgt ctgaaatatg gcttagataa ttagccttat tttcagacgg cctttgttct
     3601 agtaatttac ggtttcggac attttgtctt caacccgtcg atgtgttcca ttaagtcagg
     3661 tgggacatga ttccacatag gatcaagaat aaaatcgata ccttcccgtc tggctaattt
     3721 ggctgctggg acaaaatcgg aatcaccaga aaacaagatg attttatcgg cttgtttttt
     3781 taaggtaata gatgaaatat ccaagcctat acgcatatct acaccttttt gtttaacatc
     3841 gagcacgaca tcgttttccg taatttgagt tgacgggttt ttcagaagat ttttcatgac
     3901 ttggggtttt aatatccatg ctgtattgtc cgacagcttg cctaaacgta acgctaattt
     3961 acgcttcttc ttcaattcgg tatgcagatt gcaacgaaat atggcttccg gagtctttga
     4021 aaaatctatg gcacgatgag atacgggatt gtgcattttc ttttcaaacg gcacgcagtc
     4081 gtaaaagaaa attctatata aatcatccat ttgatctgtg ccgttgattt tatctttcag
     4141 atgcagcatc gaatatcgga aagccaaatc gagacctttg caataacata ggttactaaa
     4201 attttatgct caatctcatt ttcaaaatgc aaaacttttc tgatttttcc tactttttgc
     4261 tcaatattag gaaagtttta ggcaattgaa aattttttgg cgcattttta tgcgtcaaat
     4321 ttcgttaaca gactattttt gcaaaggtct caaatcagca attctttttg catcgtgtgg
     4381 attttgagac aggcctaatg accgtacccg cttaataaaa aatgcaccgt caatcaaaat
     4441 ggcggttttc atattgcttc ccctatattt gtcaaagata taaaaaagcc cttgggatcg
     4501 gcatacccgc tatgatttaa tgggtaatgc gtactgccaa gggcgctatt tctttttgga
     4561 ttatactgat gtagataaag cctgtcaaaa agttcgtgtg ctgaatttac ggattatcag
     4621 gattttcaga cggcattctg ttgcacgaac gggattagaa actgtaatta acccgcgccg
     4681 tataagtgcg cggcgcgccg ggcatggcgt cggaacgcca gtatttttga ttgaacagat
     4741 tgccgacggc gaaagtgagg ttcaggtttt tgcggttcca gccgagcatg gcgtcggtgc
     4801 ggacgaagcc gggaagcgtg gtgacttctt tgtttcttga atcgtagccg tagcgtttgc
     4861 ccgtaccggt tacgccgatt tcgccgtaga ggttttcggt cggcgtgtaa cggaagaaca
     4921 ggttgccggt aacgttgctg gtgttgtcca agtggatgcc cactctgtcg ggattttctt
     4981 tgtcttcaac gactttcgcc tgcatcacgc ccaacgagcc gcgcagatag agttttttgg
     5041 ggatgatttg tccgatggcg gacagttcca caccgcgcga acggtgtttg ccgccgacag
     5101 cccaagtgta gggatcattt tgcgcgtcgg gacggtagcg gatattgaag cgttcgattt
     5161 ggtaggcgga caatgtggtg ctgagtcggt cgtccagcca actgcttttg acaccggttt
     5221 cgtattggcg ggtgtattcg gggtcggcgt tgaacacggc ggaagacgaa gtgtcgatgc
     5281 tcaaatagcc gccgcgtccg ccataaggtg cgaagccttt gttatacgag gcgtagagcg
     5341 tgtgggcagg cgttacgttc cacactgcgc cgatattggg gctgaacgaa tggccgctgt
     5401 attggcggct gctgccggtg agtttgtttt cggaattaaa ggtgtatttg tcgtaacggc
     5461 cgccgaggac gaatttcaaa tcgggcgtgg cggagaagat gttttgcacg aaaatgccgt
     5521 aggagtcggc tttgtggcgg ttttgggtga ggacgggctg caatctgccc gaagccggcc
     5581 agcttgcgcg gtcgtagggg tcgatggaag cggtaaaggc gcggctgtaa cccaatgtcg
     5641 ggttgcggtg ttcgcggctg taatccaagc ctacggtcag gtggttttca aaacggccga
     5701 tgttgtagtc gccgttgagc gtgaaattag acgacagagt tttgttgtcg gtctgctgcc
     5761 aggcgtagtt gcgcttgatt aggctgccgt tttcgctgcc tccatagaag tggtcgaaat
     5821 cctgcgccgc cgtgcggtgg gcgagctgcc attgggcgcg ccatttgtcg ttgaaggcgt
     5881 attcgaggtc ggaacgccaa acttgcagct tgtctttgac aaaatcgttc gggtgggcga
     5941 aacccatgcg gtaaggcagt ccgaagcggt cgtacacgga cttggtcgga ctgcggtcgg
     6001 gcgtgcgctc cacattgtcg taggtgtatt gccccgtcca tttcaagccg ttgtcgagtt
     6061 tgacggtaat gctgggcgaa accatgacat ttttgctgtc tatgccgctg cggaacgaat
     6121 tggcgcgccc gacttcgccg gtgagacgga tggcgacgtt tttgttcaac acttcgttaa
     6181 tgtccatatt caggctgcgg tttgcccacg aaccgtaaac cgcaccgatg ttgcggcttt
     6241 gtttgaagtt ggcgtatttg ctgaccatgt tgatgacgcc gccgccgttg gtgcggccgt
     6301 aaagcacgga agacgggcct ttcaggattt ccacgcgctc gatgttggca gtactgcggc
     6361 gcacttgtcc gctttcgcgc acgccgtcgc ggtaaatatc ggatgcgtcg gcttgaaaac
     6421 cgcgcaggaa aatgctttcg ccgcgcatat cgtaggcagc gtcgatgccg gcattgcctt
     6481 cgaggatgga actcaaatcg ttcgtaccgt aatttttgtt tttctggata ttgagcgtat
     6541 cgatggtttg cggcgtttct ttgatgagct gtccgttgcg ggtaacggcg gcttcgtcgt
     6601 agttgatgta gcctttgagt acgctggtgt cggactgtcc gaccacggaa acggtgggca
     6661 gagtggcggt gtaatgttca ccattgtcct gcgtatcggc ggcagcaaca gggaaggaag
     6721 caataatcag cgtgggtaat aaagctaaat gaaatgatat tttcattttt atactcaatt
     6781 taacaaaaca accgaattat attgcctcac ggaggaaatg agaataattt cttttaacta
     6841 tattgaacat gatatttgta aacaaaggtc tcagaatgcg gaaaactcgc cgcctgatac
     6901 tgaaaaatgc cgtctgaaca gggttcagac ggcatttttt tgaccgcgaa attatgcgcc
     6961 gaacactttc aaacgttctg caacgggttc aaaggtcttt tcacctgccg ccccttcaat
     7021 accgccgata accatttgtg cgcgcaacaa ccagttttcg gggatattcc acgctttggc
     7081 aatcgccgca tcgggcaagg gattgtaatg ttgcaggttt gcacctacgc cgaccgcggc
     7141 aagtgtcgtc caaacggcat actgcaccat agcgttcgcc tggtccgccc aaacgggaaa
     7201 gttggcggca taagcaggga actgctcctg caaacctttg acaacatttt gatcttcata
     7261 aaacaaaata gttgccgcac ccgccttaaa caggttcaat ttttgcgcgg tcggttcaaa
     7321 actgtcggca ggcacgacgg cacgcagcgc gtcttcgaca aattgccaca ccttatcatg
     7381 ctcttcgcca aacagcacga ccacgcgggc agattgggaa ttgaacgaag aaggtgtgtg
     7441 caaaacggcg tgttcgacga tttggacaac ttcatctttg ccgacgggca ggtttttatt
     7501 taacgaataa acggaacggc ggctttcggc agcctgttgc agagattgac gggtcatttc
     7561 ttttcctcaa aaacattcaa caaaatacac tttaggcagt ccggttcagg ctttacgctt
     7621 gaaaaacagc ctgtccagcg accatgctcc gccgcccgct gccgcgatat agaggaatac
     7681 gaagcagaac agcactgcgg actcgccgcc gttggcaatc gggaacaaag catttccgga
     7741 agcgtgcgcc ataaaatagg caaccgccat ctgaccggac aaaacaaacg cggcagggcg
     7801 cgcaaacagg cccaacacca gcaaaatgcc gccgacaatt tctaaaatac cggcaagcag
     7861 caacagcccg ccgggcgaac cgctgcccat ttcaatgggg aaggcgaaga ttttcgacgt
     7921 accgtgcaac aaaaacaggt aggcggttac gatacgcaaa acagaaagca aaaccggttg
     7981 gatacggttg cagcaatcgg acataaaagc tcctttgata tttgtcttca tttcaggcgg
     8041 tagagtatag atgtttcccc ttttcaaata tatactgctt ttattgatac aacttttcca
     8101 cacaagaaaa tatggacacc ctgttcagcc tcaaggtttt ccgccaagtc gtccaaagcg
     8161 gcggcttcac ccgcgccgcc ggcgcgctcg gcatctccac cgcaatggca agcaaacacg
     8221 tcagccactt ggaaaacacc gtccaagcca aactcctgca ccgcaacagc cgcaacctca
     8281 gcctgaccga agctggggaa gaatactacc ggcaatgcag ttacgcgctc gacacgctcg
     8341 acgatgccgc gcaaaaagcc gccgggggga cggaaaaacc gcaggggctg ctgcgcgtaa
     8401 cgatgccact gtggtttgcc ggcagccaga tatgcaactg gctggcggaa taccgcgaac
     8461 gttatcccga agtgacatta gaactgattt tggacaaccg ccacgtcgat ttgattgccg
     8521 aaggcgtgga tttggcgttg cgcgtttccc aaaccctgtc cccgtcactg attgcgcgcc
     8581 cactggcgga aatcgaattt gccctgcttg cctcgcccga cttcctgagg cgcaacggcg
     8641 tgccggaaac gccggaagaa gtggcggggc tgcccgccgt cctgccgacc tacaccaacc
     8701 agcagaaact cgacctcacc cgcaaatcgg acggcaaaaa ataccggctt gaactgaccc
     8761 ccgtcatccg taccgacaac acgctgatga tgcgcgaaat gattaaggcg ggcgcgtgca
     8821 tcggttatca gccgctttgg gcggcggaac acgatttgcg ctgcggcacg ctggtgaggc
     8881 tgctgcccgg atacgccgtc ccgaccgacc ggctgaatgc cgtttatgca gacagggcat
     8941 tcttaagcgc gaaagtccgc agcttcatcg attttctgaa cgaaaaaatc gccagcagga
     9001 aaggctgccg aaatgccgtc tgaaaccgcc cgcccccttt atgcggacac gtgcgccgca
     9061 cacacgctcg tctggttccg ccaaaacctc cgcatccgcg acaacgccgc cttatgcgcc
     9121 gccgttgccg aaggtttgcc cgttatcggt atttggattg acgatgccga aacagacaac
     9181 cctcgccgcg ccgcgttcta ccgccaatcc gccgccgaac tcgcccaagg gcttgcaggg
     9241 cgcggcattc cgttctacac ggcggcatcg cctgccgggc ttgtccggct cgccgtccgc
     9301 ctcaataccc gcgccgtcat cgccgacgaa tcccatactt ttgccgacaa actcaccgac
     9361 aacgcccttt ggcatgagct ggacaaacac ggcatcgcgt taaccttcgt caacgaccgc
     9421 gccgttttcg gcaaaaccga cctgataccc gacggcggca cggcatatgc cgatttcgac
     9481 cgctaccgcg aagtatggct cgaccgcttt tccaagcagc cccccgccgg ttcggaccta
     9541 ttcgcggcat accgccaacc cttccccgaa aacctttccg ccccgcagcc tgccgtgctt
     9601 tcagacggca tcttcctgcc gcgaaacggc ggcgaaacgg cggcttggcg gcagtggcgg
     9661 cggtttctcg aacaggcgga ttcctactct gttttaaagg atttcccctc acgcaaaaac
     9721 acttcgctga tgggcgcgta tttgagtgcc ggctgtatct cgccgcgcct gctcgcgcgg
     9781 gaaagcctcg aacgccgtct gaacgcgtgg gtggacaaca tcatccgccg cgattttttc
     9841 cttcaacttg ccttgcagca cgcggatgac gacccttcag acggcaatcc ggaacacacc
     9901 ctgcgcctga cgctttggca gcagggccgg accggcattc cgattatcga tgccgcgatg
     9961 cgctgtttgc acaaaaccgg cagcctccac cccgccctga gacgcttgag cgcggacttt
    10021 ttctgccacg ttttaaacct cccccgccgc gaaggcgaga tatggtttgc ccggcagctg
    10081 accgatttcg atgcggcaac caaccaaggc aactggcggc ttgccgcctc acggcacacc
    10141 taccccgaca ttgccgccgc cgcacacaag accgaccccg acggcacctt tgtcagacgg
    10201 catatccccg agcttgccca cctgccggcg gcagtcatcc ataccccgtg gcgcgccgcc
    10261 ggcagcatcg acacccacgg ctatcccgct tatcctgtcg ccggtattcc ctaagcggta
    10321 cggcaaccta aaacaaatgc cgtctgaact tccgtttcag acggcattgt gtccgaatga
    10381 ctcaatatct tccgtttttc tcactttcaa taatcagata tgcccaaatc accgccaccc
    10441 aagccgacac cgttcccatc gccagcaaaa aatgctgcca atccgtcatc ggcggtacag
    10501 ggcaacggcc gcattcgatt tccacaccgc ccaacaaccg gtacagcagc atatccgcaa
    10561 tccaaagcaa caatgggtat ttcaaacgct gcaaccaaaa aaccaaccac gtccgcacga
    10621 ttcacctcac ccccattccg acaacgatat tgtaccgctt ccgtgcaatg ttaaaatata
    10681 gtggattaaa tttaaaccag tacggcgttg cctcgccttg ccgtactatt tgtactgtct
    10741 gcggcttcgt cgccttgtcc tgatttaaat ttaatccact ataagcccac ttctaccgcc
    10801 gtacaaaatg ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaat
    10861 atcggcaaaa atctgcccgg tttccgtccg cgtgcttcgc agcgggaaat gattgcggcg
    10921 gttgccaacg ctttttcgcg gacgttgacg cgcgaagaag gcggcgagcc gcccaagcgc
    10981 gaaggcgaga gcattgccgt aatcgaaggc ccgaccggcg tgggcaaatc gcttgcctat
    11041 ctgcttgcgg gcggcatcat ggcgcaaaca cgcggcaagc ggctgattgt gagcagcgcg
    11101 acggttgcct tgcaggagca gttggtagac cgcgacctgc cgtttctggt cgaaaaaagc
    11161 ggtttggaac tgaccttcgc acttgccaaa gggcgcggcc gctatctctg cccctacaaa
    11221 ctctatcaac tgacgcaaag caacgcccag caaaacctgc tcggctttga agcccccgcc
    11281 gtgttgtggg acagcaaacc caagcccgaa gaattgaagc tgctgcgcga catcgccgat
    11341 gaattttccg cccgacggtt caatggcgac cgcgacactt ggccggaaaa aatcgatgac
    11401 gcgatttggc tcaaagtgac caacgaccgc cacggctgcc tgaaaaccgc ctgtcccaac
    11461 cgtccggaat gcccttttta cctggcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt
    11521 gccaaccatg accttctgct tgccgacatc agcatgggcg gcggcgtgat tctgcctgcg
    11581 cccgaaaaca gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc
    11641 cgttttgccg ccgaacattc atggaatatt gccgtttgga cgctggaaaa actgccgcag
    11701 ctgaccggca aaattgccgc gctgaccgat aaagccgaac ttgccaacct ggccgacgaa
    11761 gccgccgcat ccttgctcga cagcctgcat gaatggcaat tccatttggc ggaagagccg
    11821 tctttaagtc tgggggtgtc tgaaaacgac agacgaacca acagcgaacc gacttggctg
    11881 tgggaagacg gcaaaatccc cgaaggcctc gaaaccaccg tttccaatac agccattgct
    11941 gcgcgcagcc tgctcaaaca cgttatcggg ctgaacgatg cgctttccgc cgcgcgccgc
    12001 gaaaaagaac aggacggcgc gctcctcgac cgcctgaccg gcgatttcgg catcttcatc
    12061 gcccgtatcg aacaaatcag cgcggtttgg gatttgctct ctaccgttcc cattgagggc
    12121 gaagagccgt tggcgaaatg gataacccgc cgcgccgacg acaaaaacga ctacattttc
    12181 aacgccagcc ccatcagcag cgcgtcccac ctcgccaaca gcctgtggcg gcgtgcggca
    12241 ggcgcggtgt tgacctccgc caccctgcaa tccttgggca actttaacct gatgctgcgc
    12301 caaaccgggc tgcaatggct gcctgaaacc accaccctcg ccctcaaaag cccctttgac
    12361 tttgaaaaac agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa
    12421 gcacacaccg ccgccgtcat cgagtggctg cccaagctta tttcgcccac cgaagccatc
    12481 ggcacactcg tcctgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc
    12541 ggagactacc tgccgctcct gctcgtacaa ggcgaattgc ccaaagccgt cctcctgcaa
    12601 aaacaccacc gggccataga agaaggcaaa gccagcatca tcttcggact cgacagcttt
    12661 gccgaaggac tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc caaactcccc
    12721 ttcgccatgc ccgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggc
    12781 ggcaacccct tcatcgaaat caccgtcccc gaagccggca tcaaactcat ccaggctgtc
    12841 ggccgcctca tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcatc
    12901 aaaacacagc ggtacggcca acaattatta gccggcctgc caccgtttaa aaggataggg
    12961 taaacctacc gcccttcccg caacagaaag gaaaagtcat gaacttcacc cgactgctca
    13021 accaagtctt aagcacggtt caaaaaaaag gcaacacatt ctccgacagc ccgctcaatt
    13081 cattcggcgg aggcgcgctg gttgccggtg tcgcctccat gctactgaac ggtaaaaacc
    13141 gcaaaaccat caccaaaatc ggttcgaccg ccgctttggg ctacctcgcc taccggggct
    13201 atcagatgtg gcagcaaaac aaagggcagg caaccgtaac acaaagcgat ttccaacctg
    13261 ccggagaaac tgaagaaaca tacagccgca ccgtattgcg caccatgata gccgccgccg
    13321 cttcagacgg catgatagac gaagccgaac gccggactat cgaacaggaa agcggcacag
    13381 accccgaaac tgccgcatgg ctcgccgccg aataccgcct gcccgcaagc atcggggaca
    13441 tcgccaccgc cgtcggcaac gatgaggcgt tggcggcgga ggcctatctg gcggcaaggt
    13501 tggtctgtgc cgatttgtcg cggaaagaaa ccgtcttcct cgcccgcctg tcgcaggctt
    13561 tgaaactgga tgacaatctg gtggagagtt tggaaaggca attggggttt tgatgacaca
    13621 gctggggagg gaaaatcatc aaaaaacggt tattccgacc ccggcctcaa aacggcaaaa
    13681 tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
    13741 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
    13801 cgtcgccttg tcctgatttt tgttaatcca ctataaaagc cgccgccccg aacggcaaac
    13861 agcggcatag gctgccaaag gcaatatccg acaagaaggc aggctactgc aatgcgccgc
    13921 cgtgggaaga tttaagcagg cgttcgtgtt tgtggaattc ccgccccata taataagaac
    13981 cgaaataggt caggtggtct tggtcgccgt aaagatagcg tccgtgtatt tcgaccgtgt
    14041 ttttgggcag gtatttttgt gcgtccaccc aatgcacatt ggggatatct ttaaccaaat
    14101 caaaaaccgc ctgattgctc ttgccgatgt cgcccatagc ctgaatgggg cggagatatt
    14161 ggtttgcggc aaatcttttc aatttttcct ccctcagggg cgaacggctg attgatgtgt
    14221 tgtttgcaaa aacatagacg ggtttgacgg cggctatcct tttgacggtt tccctgaatc
    14281 gggcttggaa cccgggtatt aggaaggatt gcgcttcaaa tctcggcacg ggctggccgc
    14341 ccatcctcaa atcatagaat tgggcaatga aaacggcttc ggctttttca acttcatccc
    14401 ggtattttcg acataacggg ttgtctgcca gcttctcatc tacccaaacc aaacactccg
    14461 aatcgaggga caggattttg gctttccacc cttcccggct gccgacataa tccagaaacc
    14521 cccgcaggtg tccggcgtgc gagtcgccga gggtcaggac ggtttccgga aaatgatttt
    14581 ccgcagcaag gggcgcgccg ggcaacgggc ggaggtgttc ctgtttcaat atcccccttg
    14641 cgtacaggtt gtaaccgaca agtatcaggg acggggcgag atagaggcag aaaaatgcct
    14701 ttttgaaggt catcttccgt tttctaagcg gctgttcaat caaataataa ctcaacaggg
    14761 aaaatccggc cgtcaacgcg gcaaccgccg atacggcagg cagtccgagc tgtttgtcgc
    14821 ctgtaatgta atgggcgaaa gcaataaaaa tccaatggta caggtatagg gaataagaga
    14881 ttttgccgac aaatacgatg gggcttgccg acaggatgcg ggtcggaagt gtcccgtatt
    14941 gcatactccg gataagcagt gccgtcagca ggcagggaag gagcagggtc attcccggga
    15001 taaacggatt gtgtttgtca atcacgaaca ggcaggcaag caatgcgccg aagcagagtg
    15061 atgaaagcaa ctgccgtttt ccatttgctg tttgccgtct gccgttttgc gtttgcccgt
    15121 aaaccgccag cagcgaacct gccaacagct cgggaaacct cagtgtcgaa aggtaataag
    15181 tattgggttg gttgagaata tcggtataaa acccgcttgg caaaaacgat gtggcagtca
    15241 aaatcagaaa taggatgatg ctgatgttac gcagcacccg tagcgatttt gtttttttgc
    15301 agcaaaatat cagcaaaaga ggatacagga ggtaatactg ttcctctact gccaaagacc
    15361 agatatgcag tacggggttc tcgtcggcac tcaaatcgaa atacccctgc tgaaacccca
    15421 gataaatatt ggacaagaaa accgcagaaa gctccacggt tttccgcatt tggttgaaat
    15481 cttcgtaaag gaagatttga gaggcaatca ccgaagccag cgacacggcc gcaataaaag
    15541 caggataaat ccgcttaatc ctgcgggtat aaaaatcccg gaaagaaaaa gaaccgttct
    15601 gtatttcaga aagaatgatg ccggtaatga ggaatcctga gatgacaaag aaaatgtcca
    15661 cccccaggaa tcctccgggc agccagcggt tattcaggtg gaaaatcatg acggatagca
    15721 cggcgacggc ccgcaatccg tcaatttccg gtctgtatcg gacagcttgc ataaatatcg
    15781 cccccgtatg tcccgttatc ttaaaaatgc cgtctgaacg cgcgttcaga cggcatcggt
    15841 ttcagtaaac gctcaaatcc gtttcgccaa cgcttcagcc ttgcccacat acagcgcggg
    15901 ggtcagctca agcaatttgg ctttggcttc ggcggggatt tccagcaatc cgataaagcc
    15961 tttcagcact tcgggcgtga tgccgccttt gccgcgcgtc aggtctttca gtttttcgta
    16021 aggattggcg acaccgtaac ggcgcatcac ggtttgaatc ggctcggcga gcagttccca
    16081 agtggcatcc aaatcagcgg caagcgcggc agggttgggt tcgagcttgt tcagaccgcg
    16141 caggtgggcg gcgaaaccca acacggcata gcccacgccc acgcccatat tgcgcaatac
    16201 ggtgctgtcg gtcaggtcgc gctgccagcg ggaaatcggc agtttttcgg acaaaaagcc
    16261 caatacggca ttggccatac cgaggttgcc ctcggagttt tcaaagtcga tggggttgac
    16321 tttgtgcggc atggtcgatg agccgacttc gcccgctttg actttttgtt tgaagtaacc
    16381 caatgaaata taaccccaaa cgtcgcggtt aaagtcgatc agaatcgtgt tgatgcggct
    16441 gagggtttgg aagaattccg ccatatagtc gtgcggctcg atttggatgg tgtaggggtt
    16501 gaaggtcaga ccgaggctga tttcgacgaa gttgcggcag tgggtttccc aatctacatc
    16561 aggataggcg accatatggg cgttgtagtt gccgaccgcg ccgttgattt tgccgaggaa
    16621 ctcttgcgct tgcaggtttt taaactggcg ttgcaggcgg tacacgacat tggcggtttc
    16681 tttgcccaaa gtggtcggcg tggcgggctg gccgtgggtg cggctcatca tcgggacggc
    16741 ggcaaggtcg tgcgccatag cggtcagttt ttcgatgatt tcggccagct tcggcagcaa
    16801 aacagcctca cgcgcttctt gcagcattaa agcgtgggac aggttgttga tgtcttcgct
    16861 ggtgcaggcg aagtggatga actcactcac ggcggcgact tccggcactt cggcaaaacg
    16921 ttttttcagc caatattcaa tggctttgac atcatgattg gtcgtggctt cgatggcttt
    16981 gacggcggcc gcgtcttcca atgaaaagtt ttcaatcacc gtgtcgattt cggcaagcgt
    17041 ttcggcactg aagggcggca cttcggcaat cttcggctcg gcggcgaggg ctttgagcca
    17101 gtttaattcg actttgacgc gcgctttcat caggccgtat tcggaaaaaa tcgggcgcaa
    17161 tgcttcaacg gattgggcat aacggccgtc taaaggggaa agcgaggcga tggggttgat
    17221 catttcggca tcctgttcgg gaaagacatc aaaaattgtt aaattgtttg caattataca
    17281 tcactctcag gacgctatgc cgtctgaagc ccgtgttccg acagatacgc aacgatttcg
    17341 tcttcatcgt ccgatatggc gaacaaagag gctgcccctt cggaaatcag accgcgcgcc
    17401 aaaagctgcg cgtttatcca ctccgccaag cccgaccaaa acgcctttcc gaccaaaaca
    17461 atcggacgcg gcggcacttt gcccgtctgc accaaggtca ggatttcaaa caattcgtcc
    17521 agcgtcccga agccgcccgg catcacgaca tatgcttggg aatagcggaa aaacaccgcc
    17581 ttgcgttcgg caaaacggga aaaccgcaag gcgatgtcct gatacggatt cggtttctgc
    17641 tcgtgcggca aaacgatgtt cagccccacc gaaaccgact tccctgcaaa cgcgcccttg
    17701 tttgccgcct ccataatccc cggcccgccg cccgaaatga cggcaatgcc cgaatccgac
    17761 agccgccgcg ccagacggca ggcgaacgca taatccgcat gattctgcgg cgtgcgcgcg
    17821 ctgccgaaga tactgactgc cggaaacacg cccgccaatg cttcgtctgc ctgcctgcgt
    17881 tcggcatcat aacgtgcctg ctccggcaca cggtttgtat tctccattcc atcctccgtt
    17941 caaaaacagc gattgtacac cgtcaaaaac gtatagtgga ttaacaaaaa tcaggacaag
    18001 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc
    18061 ttagagaatc gttctctttg agctaaggcg aggtaacgct gtactggttt ttgttaatcc
    18121 actataacgc aagcaccgca aagacgcgcc aaccctctcc caaccttttt tcagacggca
    18181 ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac agaaaaatcc gaaaaatgaa
    18241 tattttttac gaagagtccg gccaattcaa aatcgccgcc atcgtccaaa aaaacgacgc
    18301 cacctaccaa gtcgataccc cacacggcaa acgcaccaaa gtgaaggcga acaacgtctt
    18361 tgccgagttt gacggcgata tggcggcgtt tttggaaaac gcgcaggcac aggcggcgga
    18421 catcgacacc gatttattgt gggaagtatg cggcgaagag gaatttaccg ccgaagccat
    18481 cgccgaagaa tattacggcc atgcgccgac caaaaccgag ctggcggcaa ctttgattgc
    18541 gctttacgcc gcgccgatgt atttctacaa aaaagccaaa ggcgtgttca aagccgcgcc
    18601 cgaagaaact ttaaaacaag cacttgccgc catcgaacgc aaaaaacagc aagacgcgca
    18661 aatcgacgct tgggcggaag ccttgaaacg cggcgagatg ccgtctgaaa tcgcggcgga
    18721 tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg acctacaaag cctttaccaa
    18781 agccgccgac gcgctgaaaa cctctgccta cgaattggcg aaaaaaacgg gcggcattac
    18841 gtccattccc caatacctgc aagacggatt tgaaatcaaa tacttcccca aaggaacagg
    18901 cttccccgac cttgcccttc ctgaaatgcc cgacctgccc aaggctgacg ttaccgcctt
    18961 ttccattgac gacgaatcga ccaccgaagt ggacgacgct ttaagcctga ccgacttgga
    19021 caacggcacg aagcgcgtcg gcatccacat cgccgcgccg tcgcttgcca ttaaacaagg
    19081 cgacaaaatg gaaaaaaaca tcatggaacg cttgagcacg gtttatttcc ccggcggcaa
    19141 aatcacgatg ctgcccgaaa actggatagc cgcgttcagc cttgatgcag gcgcgtaccg
    19201 tcctgccgtc agcatttatt ttgatgtgga cagcgggttc aacgccggcg aaccgacctg
    19261 caaaatcgaa gcggtcaaca tcgccaaaaa cctgcgcatc caagccatcg agccgcattt
    19321 caacgccgaa accggcttgg acgaagcagg cgaaatgatg ttcgtccacc atcaagacct
    19381 gatttggttc caccaatttg ccgtcgccct gcaaaaagca cgcggcaaat acgagcccaa
    19441 ccgtgcgccg caatacgatt acagcatcga attggataaa gaagacaacg tatccgtcgt
    19501 ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc gagatgatga ttcttgccaa
    19561 cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc ggcctcttcc gcgtccaacc
    19621 caccggaaaa gtacgcatga gcaccaaatc cgagccgcat atcggcatgg gcgtgcggca
    19681 ttacggctgg tttacctcgc cgctgcgccg cgccgccgac tacatcaacc aaaagcagct
    19741 gatcagcctg atagacgaca ctgccgagcc gctgtatcaa aacagcgatg ccgagctttt
    19801 cgccgcactg cgcgactttg atgccgccta taccgcctac gccgacttcc aacgccaaat
    19861 ggaagcctac tggagcctgg tttacctgca acagcaaggc acgagcgagc tgacggcgac
    19921 gattctgaaa gaagacctcg tccgcatcga aggtctgccg ctagtaacac gcgcgaccgg
    19981 tattccgttt gacgcgctgc ccaaatcgca ggcattgttc aaaatcaccg aattagatgc
    20041 cgagaagcag tttgtctcgc tcaactacat caaggcagtc gcacccgcgg gtacaacggc
    20101 aggcaatgcc gtctgaagcc cgatacggca aactccggca aaaacagaaa cctgaatctc
    20161 atcattccca cagtcgggaa tccggttttt tgggtttccg ttgtttttcc gtttcaatga
    20221 acttccaagc cgccgttccc gataaatacc cgcaatctaa aatcccgtca ttcccacgca
    20281 gatgggaatc taggtttatc cgcacagaaa ctgata
//
LOCUS       DAXIHS010000032        20138 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXIHS010000032 DAXIHS010000000
VERSION     DAXIHS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.032"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..201
                     /locus_tag="ACFOF2_001012"
     CDS             <1..201
                     /locus_tag="ACFOF2_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC7107351.1"
                     /translation="AQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQS
                     HLKAMCLNLLKAANRLSAPAAA"
     gene            253..1206
                     /locus_tag="ACFOF2_001013"
                     /pseudo
     CDS             253..1206
                     /locus_tag="ACFOF2_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1254..2327
                     /locus_tag="ACFOF2_001014"
     CDS             1254..2327
                     /locus_tag="ACFOF2_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC7107352.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTSLAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTFLMNVNPIFFITIPAILTAV
                     VFTLYLFTFVPIFRANAFTGDPE"
     gene            complement(2373..4562)
                     /locus_tag="ACFOF2_001015"
     CDS             complement(2373..4562)
                     /locus_tag="ACFOF2_001015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC7107353.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4701..5483
                     /locus_tag="ACFOF2_001016"
     CDS             4701..5483
                     /locus_tag="ACFOF2_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC7107354.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAKSAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5896..7830)
                     /locus_tag="ACFOF2_001017"
     CDS             complement(5896..7830)
                     /locus_tag="ACFOF2_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC7107355.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7896..9074)
                     /locus_tag="ACFOF2_001018"
     CDS             complement(7896..9074)
                     /locus_tag="ACFOF2_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC7107356.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            9300..9689
                     /locus_tag="ACFOF2_001019"
     CDS             9300..9689
                     /locus_tag="ACFOF2_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC7107357.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLTEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9972..11018
                     /gene="adhP"
                     /locus_tag="ACFOF2_001020"
     CDS             9972..11018
                     /gene="adhP"
                     /locus_tag="ACFOF2_001020"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC7107358.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(11369..14854)
                     /gene="smc"
                     /locus_tag="ACFOF2_001021"
     CDS             complement(11369..14854)
                     /gene="smc"
                     /locus_tag="ACFOF2_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC7107359.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLTAADKATAQHQSLQAQQDETAAQVQALNDEVRALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQTQEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQIQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDYAAAPQLWQHITAPTEWQHALSVILAERLHARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLNYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSETQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIVRELAQLAEE
                     QTVLQHASDGLSDDIAILQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVHTLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            14856..15107
                     /locus_tag="ACFOF2_001022"
     CDS             14856..15107
                     /locus_tag="ACFOF2_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107360.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            15104..15838
                     /gene="lpxH"
                     /locus_tag="ACFOF2_001023"
     CDS             15104..15838
                     /gene="lpxH"
                     /locus_tag="ACFOF2_001023"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC7107361.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLENAENAV"
     gene            16094..17680
                     /locus_tag="ACFOF2_001024"
     CDS             16094..17680
                     /locus_tag="ACFOF2_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC7107362.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWSFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLSFIVPWSEIRKNLGFVAIA
                     VFSCTLPYVALAMVNEEFPSLVAGAIGLMFSVFAANKGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(17848..18051)
                     /locus_tag="ACFOF2_001025"
     CDS             complement(17848..18051)
                     /locus_tag="ACFOF2_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC7107363.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            18167..18664
                     /locus_tag="ACFOF2_001026"
     CDS             18167..18664
                     /locus_tag="ACFOF2_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC7107364.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            18746..19939
                     /locus_tag="ACFOF2_001027"
     CDS             18746..19939
                     /locus_tag="ACFOF2_001027"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC7107365.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAIRAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
BASE COUNT         4373 a         5772 c         5309 g         4684 t
ORIGIN      
        1 gcgcaaacca aacgcaaccg atatttgtcg aagacccgtt atgtggttga acaaagcttc
       61 ggtacgttac accgcaaatt ccgctatgcg cgggcggcct atttcggact gattaaagtg
      121 agtgcgcaaa gccatctgaa ggcgatgtgt ttgaacctgt tgaaagcggc caacaggcta
      181 agtgcgcccg ctgccgccta aaaggcggcc cggatgcctg attatcgggt atccggagag
      241 gattaagggg ggatttgggt agaattaggc ggtatttggg gcgaaaacag ccgaaaacag
      301 ctgaaaacag ctgaaaacag ctgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
      361 ggaattttgc aaaggtctca ggtcgcactc gtcgtgacgg aaagtaccgg cggtctcgaa
      421 atccccgccg ccaaagccat ccgccgagca ggcatagccg tgattatcgc caacccgcgt
      481 cagacgcatc agtttgccca atcgcagccg ctgaccaaaa ccgacgccaa agatgccaaa
      541 atgcccgcct tcttcacaca gatgatgacg cagaaagaag attggcaaac catgccctac
      601 caaccgccca ccgaagcgga agaagtgttg gaagccttgg ttaaccgccg caaccaatcg
      661 gcggatatgc ggactgccga gaaaaaccgt ctgcatcagg ttcatgaaac gcaagtcgga
      721 agcgtcaaac aactgattgc ccattttgac cggctgattg acgaattgga caaacaaatc
      781 gacgaccaca cccacacgca ttttgacggc aaagcccaag tggcagaaca aatcaaaggc
      841 atcggttcga taacgacggc tacgctgatg gcgatgctgc ccgaattggg gcggctgtcg
      901 cacaaacgga tagcgagttt ggccggcatt gccccgcacc cgagggagag cgaggaaacc
      961 aaattcaaaa gccgctgctt tggcggaagg tctgcggtgc gtaaggcact gtatatggct
     1021 accgtggcag cgacacgttt tgaaccgctt attcgggatt tctaccaacg cctgctgtcc
     1081 aagggtaagc cgtataaggt tgccgttacg gcatgtatgc gcaaactgct gacgatatcg
     1141 aatacccgga tgcgtgatta ttttgccgaa aacgataccg ccgaaaacgg tatccggacg
     1201 gcttgatttg agttttggta tttttgcccg acggggtgaa aaatacagtt gctatgcggc
     1261 cgtttttcgt cggcgcggcg gtgcttgcca tactcggtgc gctggtgttt ttcatcaacc
     1321 ccggtgccat cgtcctgcac cgccaaattt tcttggaact tatgctgccg gcggcatacg
     1381 gcggtttttt gactgcggct ttgttggact ggacgggttt ttcgggtaac ctgaaacctg
     1441 tcgcgacttt gatggcggca ttattgctcg ccgcatccgc tatactgccc ttttcgccgc
     1501 aaactgcctc gtttttcgtc gccgcctatt ggctggtgtt gctgctgttc tgcgcccggc
     1561 tgatttggct agaccgaaac accgacaact tcgccctgct aatgttactt gccgcgttca
     1621 ctgtttttca gacggcatat gccgtcagcg gcgatttgaa cctgttgcgc gcgcaagtgc
     1681 atctaaatat ggcggcggtg atgttcgtat ccgtgcgcgt cagtattctt ttgggcgcgg
     1741 aagccctgaa agaatgccgt ctgaaagacc cagtattcat ccccaatgtc gtctataaaa
     1801 acatcgccat taccttcctg ctcctgcacg ccgccgccga actttggctg cctgcgcaaa
     1861 ccgccggttt tacctcgctc gccgtcggct ttatcctgct tgccaagctg cgtgagcttc
     1921 accatcacga actcctgcgc aaacactacg tccgcactta ttacctgctc caactctttg
     1981 ccgccgcagg ctatttgtgg acaggcgcgg cgaaattaca aaacctgccc gcctccgcgc
     2041 ccctgcacct gattaccctc ggtggcatga tgggcagcgt gatgatggtg tggctgaccg
     2101 ccggactgtg gcacagcggc tttaccaaac tcgactaccc caaactctgc cgcattgccg
     2161 tccccatcct cttcgccgcc gccgtctcgc gcactttctt gatgaacgtg aacccgatat
     2221 tcttcatcac catccccgcg attctgaccg ccgtcgtgtt cacgctttat cttttcacgt
     2281 ttgttccgat ttttcgggcg aatgcgttta cgggcgaccc ggaataaaaa tgccgtctga
     2341 aacggtttca gacggcatcg tattgccgat aatcaagcct cctgcggatc gacatccacc
     2401 gaccatctga ttttgccgtc gcggttttgc tgcaacacct gcacccacaa actcacggca
     2461 cggtgcaaat cctgtcggga tgtcgattcg aggaagattt gcgcgcgttc gcgttcggcg
     2521 aggcgcacca tcagcatcgg ggccgcgccg aactgggaaa cgctttcggg caacagcggg
     2581 gcgagggttt ctttggcggc attgagaaat tccatcgcat cggcaacgcg cggcgcatcg
     2641 gcgcggacgg cggtctggaa accgaagggc ggcatggcga acatttgccg ctcgttcaat
     2701 tcgttttcgg caaacacggc atagtcttgc gctttgacgg cggcgaagac aggatgttcg
     2761 ggcagttggg tctgtatcaa caccttgccg ggtttgtcgg cgcgccccgc cctgcctgac
     2821 acttgcatca gctcggcaaa cagcctttcc ggcgcgcgaa agtccgcgct gtacaggctg
     2881 ccgtcggcgt tcaatacgat aacgaggttg agccgcgcga aatcatggcc tttggcaagc
     2941 atctgcgtgc cgaccaaaat gtcgattttg ttgtcggcga tgcggcggta caaatccgcc
     3001 cagtcatttt tgtgcgcggt gctgtccctg tcaacacgga cgacggctgc cttgggcagg
     3061 aaggtgcgca gggtttcttc gacgcgctgc gtgccgtggc cgacggcggt caggtcttgg
     3121 ttgccgcagt cggggcattt gtacgggatg ggttcgcggt ggtcgcagtg gtgacagcgc
     3181 agttggcggg cgcgttggtg cagtaccatt ttggcggagc agtttgggca gccgaaggta
     3241 tggccgcagt cgccgcaaaa cagcgcgggt gcgaagccgc gccggttgag gtacaccagc
     3301 gacataccgc ccgcttcaaa attctgtttc aaaagctgca aggcttgcgg cgagaaaccg
     3361 ttgtcgagtt tcagacggcc tacgttgaga atgtccactt gcggcagttg cgcggcggta
     3421 tgggcgcgtt cggtcagttg cagcaggcgg tacgcgccgc tttgcgcctt gtgccagctt
     3481 tccaagctgg gcgtggcact gcccaatatg atggggcagc cgccctgctt cgcccgccac
     3541 accgccaaat cgcgggcgtg gtagcgcaat tcgttgtctt gtttgaacga gccgtcgtgt
     3601 tcctcatcga ctacaatcag cccgacatca tccatcggcg taaacaccgc cagccgcgtg
     3661 ccgatgacca atttcgcctg ccccaacatc gcgcgcaaat aatcctgcgt gcgcctgcct
     3721 gccgccatct gactgtgcaa cacggcggtc ggcacgtcgg caaaacgatt tcccacccgc
     3781 ttcaaaagct gcggcgtgag gttgatttcg ggcaacagaa acaacacctg ccgcccctga
     3841 gccaacactt tcgccatcgc atcgaaatac acttcggtct tgccgctgcc ggtgatgccg
     3901 tacagcagaa acggctggaa gcgtcccaat gccgtctgaa tggcatcgga agccttctgc
     3961 tggtcagcat ttaatacaaa ttcagagtgc gaagcctgcc cattacacga ccttaaaacg
     4021 ggcttcgccg cttccgtcgt ttcaatccag ccctgctccg cccaatcttc gattaatttc
     4081 gccgcctgcg cgttcacctg cttcaacgcc gccatcgtca tcccgcccga cagcagcgcg
     4141 tcccacaaag ccgctttttt gttgaaccgc gccggcggcg gcgtttgcgc cctgcccgct
     4201 tcgttcaacg cataaaacaa cggcggctgc ggcatttcca ccgcgcgcgt ttccttcaaa
     4261 ccctgcggca gcgcggcaaa caccgcctgc cccgtcggat agtggtaata acgcgacgta
     4321 aacgagagca aatcacgcca gctttcaggc aacggcggct cgtctaaaaa ggtcgtctga
     4381 atactcaaaa tccgcgccgt atccatatcc ggcgcaatat ccgtttccca caccatcccg
     4441 accacggttt tattgcggaa aggcacaagc acccgcgttc ccggaggaag cggatcggaa
     4501 tgggaataag tcaaaaggcc gtctgaaagc ggcacgttta cggcgatgcg gtggtagatc
     4561 atacaggcag gcaaatcatt tttttgcgta ttttaacagg cggaagctat tttcagacga
     4621 taaattgagc ctaggcgcaa tgcaggcgca acagagattt gatacaattc cgcttttcca
     4681 acacgcgcaa tcatctttat atgaaaacca agttaatcaa aatcttgacc ccctttaccg
     4741 tcctcccgct gctggcttgc gggcaaacgc ccgtttccaa tgccaacgcc gaacccgccg
     4801 tcaaagccaa gtccgccggc aaatccgttg ccgcctcttt gaaagcgcgt ttggaaaaaa
     4861 cctattccgc ccaagatttg aaagtgttga gcgtcagcga aacaccggtc aaaggcattt
     4921 acgaagtcgt cgtcagcggc aggcagatta tctacaccga tgccgaaggc ggctatatgt
     4981 tcgtcggcga actcatcaac atcgacacgc gcaaaaacct gaccgaagaa cgcgccgccg
     5041 atttgaacaa aatcgacttc gcctccctgc ctttggacaa agccatcaaa gaagtgcgcg
     5101 gcaacggcaa gctgaaagtc gccgtcttct ccgaccccga ttgtccgttc tgcaaacgct
     5161 tggaacacga gtttgaaaaa atgaccgacg tgacggttta cagctttatg atgcccattg
     5221 ccggcctgca ccctgatgcc gcgcgcaagg cgcaaatctt atggtgtcag cccgaccgcg
     5281 ccaaagcgtg gacggattgg atgcgtaaag gcaaattccc ggtcggcggc agcatctgcg
     5341 acaatcccgt cgcggaaacc acttccttgg gcgaacaatt cggcttcaac ggcacgccga
     5401 ccctcgtctt ccccaacggg cgcagccaaa gcggctacag cccgatgccc caactggagg
     5461 aaatcatccg caaaaatcaa taatccatcc gcaaaaaagg cttggaaaat tccaagcctt
     5521 ttttgcggga aatgccgcct gaaaaccaat ccgccctgaa agttagcaag ccaacaaaca
     5581 catcctaacc cattgaatga ttgaaaataa taaattgaac aatacgaagc cccgccgccg
     5641 ttcccacgcg ggcgggaatc tggaatattg agttaccgtg atttatccga aacaagcaaa
     5701 gccgaacgaa ccgaattccc gtctttacgg gaatgacggg actcgagcgt tgtgtgcaat
     5761 catcgaaccc ccgccgccgt cattcccacg caagtgggaa tttggaacgt agaatctaag
     5821 aaaccgtttt tgtccaataa gtttctgctc caaaatgacc ggttctgcag cttcaggcgg
     5881 catctttgtc caacctcaat cctgtgccaa tgcgtctatc ggattgagtt tggctgcttt
     5941 attggcaggc ataaagccga acgcgatgcc gattccggtc gagcaggcga ccgccccgat
     6001 aacggatgcc gccgaaatgt ccatcgggaa gtcggttaca aaatgattga acacgaggct
     6061 gacggcggcg gacaaaccca cgccgaccaa accgccgatg acgcagatta acaccgcctc
     6121 aatcaaaaac tgctgcaaaa tattgccgcg ccgcgcgccg attgccatcc gtatgccgat
     6181 ttctttggtg cgctcggtaa cggacaccag catgatgttc atcacgccga tgccgccgac
     6241 taccaatgaa atcagggcga tggaggaaat cagcagcttc atcgtaccgg tggtgctttc
     6301 gactatctgc ctgatgctgt cgctgttgtt catgaagaaa tcttccgtgc cgtgccgcgc
     6361 tttgagcaga tcggtcagcc ctttttcggc aacctgggta ttggcattgt ctttgatttt
     6421 gacggtgatg gagttggtgt ggctctcgcc tgtgatttgg tgcatcaccg tcgtataggg
     6481 cgaccaaagc atcagcacgt cggaattgcc gaaagcgttt tcgtcttttt tcatcacgcc
     6541 gatgacggtc aaggggcgtt tcctgaacaa aatggtttta cccaacggat ccgagtccgc
     6601 aaagagtttg tctttgacat tttggtcgat gacgacgacc tgcgcgtctt ctttcacatc
     6661 gttttcgtca aacagccgcc ccgtttccag cttcagtccg cgcacgtcga aatattgttc
     6721 gcccacgccg tacaaagaag cggtcaggtc ggtattgcgg taagtcagcg tgccgccgct
     6781 cgaagtcatg ggcgtggcgg aagcaacgta gctttgtttg gcgatgattt ttgcgtcgtc
     6841 tatggtcagg gttttaatcc tgccgctgcg cctgtcgccg aagccgcgcc ccgggaagat
     6901 gctgatggtg ttcgtcccta tcgaactgat gtcttcaagg atttttttct gcgaaccgtt
     6961 gcccaatgcg acgacggaaa ccaccgacgc gatgccgatg atgatgccga gcatcgtcag
     7021 aagcgaacgc attttgtgcg ccaatactgc ttgcaccgac attctgaagg cttcgacaaa
     7081 ctggtcgtaa taaaacgacc acgaggcttt ttcccgaatc ctcccgacat tgcttgcggg
     7141 gatttcggga tttttcgagg tgtcggaaat gatttcgccg tcccggattt cgatgacgcg
     7201 gttggcattg gcggcgatgc cggggtcgtg cgtgaccata atgacagtat gcccggcttc
     7261 gtgcagcttg tggatgattt ccatcacgtt tttgccgctg gcggtatcga gtgcgccggt
     7321 cggctcgtcg gcgaagatga tttctccgcc gttcatcagg gcgcgggcga tggagacgcg
     7381 ctgctgctgt ccgcccgaga gttcgccggg cttgttgccc tctttgcttg ccaaacccaa
     7441 atcctgcaac aacttctccg cacgcgcgga acgctctttg ccgcccgcgc ccatatagac
     7501 ggctggcagc gcgacgttgt cccttgcggt cagcgagctt aagaggttgt agcgttggaa
     7561 gatgaaaccg aagcgttcgc ggcgcaatgc cgccagctcg tcaggctgca ttttggcagt
     7621 ttcgatgtcg tcgattcggt acgaaccgga accggcggta tccaaacagc cgagtatgtt
     7681 catcagcgtg gacttgcccg aaccggactg cccgatgatg gcgacaaaat cgcccttctc
     7741 tatcgacagg ctgatgtctt tcaaaatatg gacgcggttc tcgccgctgc cgaaatagcg
     7801 gttgatgttt ttacattcga tcaagctcat aatattccgt aaatcaataa caaatgccgt
     7861 ctgaaaccgt ttccgtgttc agacggcata ttcgtttatc ggcgcggcgg gccgcctagg
     7921 gcgcgttcgc cgctttcctg ctgctcggcg gcggttattt cggagatgac cactttgtcc
     7981 ccctctttca acccgctttt tacttcggta ttcatactgt ctctcatacc ggtccggatt
     8041 tcgcgttccg ccgccttgcc gtccgcaccc aacacgcgca caaacgcctt gccgccgcga
     8101 tttttcacgg tcagcgacgg aataatcagc acatttttca cgccgtcgat ttcaaccgta
     8161 ttctgcgtcg tcatccccgt ggcgagtttg ccgtccggat tcggcacaaa cgaacgggca
     8221 taatagtaga ccgcattgga agccgtatcc gtactgctgt tgtaaccgcc cgacgacatc
     8281 gtggtcagcc cggggtcgac gctgtcgagc ttcgccttaa tcggcgtatc cggttcggac
     8341 aaaatcgtaa acgaaatatc ctgccccgcc ttcaccttgg taatatcgcc ctcggcaatc
     8401 tgcattttgt tcaacatcat atccagattc gccaattgga caatcgtcgg cgtagactgc
     8461 gccgcgttca cagtctgccc ctcttccacg agaatcgcca ccaccgtgcc gtccatcgtt
     8521 gcggtaatgc gcgtgtagcc caattccgac tcggcggtat tgatggaaat tttgctctgt
     8581 ctgattagag ccttcagctc ggcaacattg gctttggcgg cggcaaacgc atcctgcgcg
     8641 ctttccaaat cctctttgga agtcgcatca tccttccaca acgccgcctg acgcttatat
     8701 ttcttctccg cactgcccaa tgcaatctgt gccgacacca gcttcgcctg atacgtttcc
     8761 aacttggatt tttccgtatt gagcgtattg gtctgcgagg tcgaattgat ttccgcaatc
     8821 aaatcgccct ttttaacctg ttgcccgagt ttgacataaa gtttcttaat ctgccccgat
     8881 gcctgcgcgc cgaccgatac caggttggac ggcgaaatct cccctgttgc agaaaccgtc
     8941 cggctgatgt cgccgcgcct gaccgtttcc gtaatataag cagcctgcgg ctcgggcttc
     9001 agataagacc atccgcccca aaccgctgcc gccgcgaccg ccgcaacagc cgcccatttc
     9061 atcatttttg ccatgttttc aattctctgt atgcgtcaat cccataaatc ggaagcggat
     9121 tctaccgcaa gtccctgccc catccaaggc ggcaaactgc cgacaataaa aataaccatt
     9181 tgttttaaat ggcttataca atctgctttc ccgaaaattt caatccgttt acatattccg
     9241 ccatttccga tacggcgtta taatgccgcc gaccgacaaa caaatggaaa cacattgcta
     9301 tgaaaaacgt tcaaaaaggc tttacgctgc tcgagctgat gattgccgtc gccatcctcg
     9361 gcatcctgac gctcatcacc tacccttcct ataaaaccta catccggcgc gtccgcctga
     9421 cggaagtcag gacgaccctg ctgcacaacg cgcagaccat ggaacgttac taccgccaaa
     9481 aagggacgtt taaaacctac gacaaaaaca aactgaaaca aaacgaatat ttcaagatta
     9541 ccttaagcga agtcagcccc gaccacttca cccttcaggc agcccccgat ccgatgacca
     9601 acgaaggcga aacctgcgtc gttacgctca acgacggcgg taccattgcc gcttccggta
     9661 caaaccaatc ctgccccggc ttcgactaat cctccgccga agcccgcaaa cccgtaaaga
     9721 tgccgtccgg acacagcttc agacggtatc tttacgggtt tgccgtccgg tttttcaaac
     9781 ttgaaacctg ccgcccctca tccggcaacc gccccgatgc cgtgcctttc caagatgtta
     9841 taatataaca tataatctat atttttcaac aacataccac ttgacctgtg agatgcggaa
     9901 gcgtatgctt acccttatat cggacaacat tccgtttaca ggtttttttg ccgtaactaa
     9961 ggagcgcaat catgaaaatg caggcagttg ttgtgaataa aaatgtagcg ggtgatgtag
    10021 aagtaatcga acgcgaggtt cgcccgttgg aatacggcga ggcattggtc gaagtcgaat
    10081 attgcggcgt gtgccacacc gacctgcacg ttgcggcagg cgactacggc gaaaaaccgg
    10141 gccgcgtgct gggacacgag ggcatcggtt tggttaaaga agttgccgac ggtgtgaaaa
    10201 atctgaaagt cggcgaccgc gtcagcatcg cctggctgtt ccaaagctgc ggctcgtgcg
    10261 aatactgcaa taccggccgc gaaaccctgt gccgctctgt attgaacgcg ggctacaccg
    10321 ccgacggcgg tatggcgacc tactgcatcg tgagtgccga ttacgcggtc aaagtcccag
    10381 aaggtttgga tcctgcgcaa gcttccagca ttacttgtgc cggcgtgacc acttataaag
    10441 ccattaaagt ttccagtgtt cgtccgggac agtggattgc catctacggc gcgggcggtt
    10501 tgggcaactt gggggtccaa tacgcgaaaa aagtattcgg cgcgcacgtt gtcgccatcg
    10561 acatcaacga cgacaaactg gcgtttgcca aagaaaccgg cgcggatttg gttgtcaacg
    10621 ccgccaaaga ggacgctgcc aaagtgattc aggaaaaaac aggcggcgca cacgctgcgg
    10681 tcgtaaccgc cgtatctgct gccgcattca actctgccgt gaattgcgtc cgcgcgggcg
    10741 gacgtgtggt tgccatcgga ctgccgccgg aatcgatgga tttgtccatc ccgcgtttgg
    10801 ttttggacgg catcgaagtg gtcggctctt tggtcggcac gcgcaaagat ttggaagaag
    10861 ccttccaatt cggcgcggaa ggtttggttg tgccgaaagt ccaactgcgt gctttggatg
    10921 aagcacccgc cattttccaa gaaatgcgcg aaggcaaaat caccggccgt atggtgatcg
    10981 atatgaaaaa agaatgcggc tgcggtcatc accactgatt tgacgtggcg atacacatcg
    11041 aaatgccgtc tgaacgctgt tcagacggca ttttttatgg atttggattt gattttaatc
    11101 tgttctgttt gaaataccgt ctgaaaaccc gtatcgcaac acgtcattat agtggattaa
    11161 caaaaatcag gacaaggcgg cgagccgcag acagtacaaa tagtacggaa ccgattcact
    11221 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgagcca acgctgtacc
    11281 gatttaaatt taattcacta taccgtctga aaacctgtat cgcaacacgt cattaaaaac
    11341 ggcaagattc agccgttctg caacccgttc aaacggcttc cgccatttcc aacgcctgct
    11401 tgatgtccac ggcgacgacg cgcgatacgc ctttttcctg catggttacg ccgaccagct
    11461 gctctgccat ttccatggtc aggcggttgt gggagatgta gaggaactgg gtttgcgccg
    11521 acatttcttt gaccaggttg cagaaacgcg aggtgttggc gtcgtccagc ggggcatcga
    11581 cctcgtccaa aaggcagaac ggagcggggt tgaggctgaa cagagcgaac acgaggctca
    11641 tggcggtgag ggctttttcg ccgccggaga ggaggtggat ggtgctgttt ttcttgccgg
    11701 gcggacgcgc cataatggac acaccggcgg tcaaaaggtc gtcgcctatc attttgagag
    11761 tggcttcgcc gccgccgaac agggtcggga agaaggtttg gactttgccg ttgacggcgt
    11821 cgaaggtttc tttgaaacgc gctttggttt tgtcgtcgat ttgggcgatg gcttcttcca
    11881 aaatggtgat ggcggcctgc acgtcttcgc tttggctgcg gtagtagccg tcgcgttcgc
    11941 gcgcttcttc gagttcttgc agggcggcga ggttgaccgc gccgagggct tcgatttgtt
    12001 gcgagaggct gccgatgctg ctgttcaata ctttcggcga ttctttcgcc aacgcttcga
    12061 gcgcgtccaa atcggcggcg cgttcggtca ggttttgatg gtagcgtttg gcgttaatca
    12121 gggcttcctg ctgctgcaac agggcggttt gggtggcagc ctgaagctgc ggcagcttgg
    12181 tttgcaaggt ttgtacgcgg gcgtattgtt ccctgccctg ctcctgaatc tgcgcgagtt
    12241 tttcctgcac aacaatatat tcttcgtcca gcgtgtgtac ggcttcggtc agctcgtcga
    12301 gcttgatatg ctgctcgtcg ttttggaact cggtttcata ggcgagggca agctcttgct
    12361 ggcgttcctg ccagtctagg gtttgctgtt cgagttgggc aatttgctgt tggtagtttt
    12421 gtttttgctg gttgagcttg tggacggcga cttcggcaag cccgtattgg cggttggctt
    12481 ccaacaaggc aagctgcgcc tgtttcagac ggccttgctg ctcttggcgg ctgtgtgcgg
    12541 cggtttgctg ctggtgttcg agttcggcgg cggcttcctg caaaatggcg atgtcgtctg
    12601 aaagcccgtc ggacgcgtgt tgcaacacgg tctgttcttc cgccaactgc gccagttcgc
    12661 ggacgatgtg ttcgcggcgg atttgccctt ggttggtgcg cgccagaagt tcggcggcgc
    12721 gttgctgcgc ttggctgtat tggcgcgtgt gttgttgttg ctgctgcatc aggtttttat
    12781 gctgcgtttc agacgagcgc acggcggctt ccgcctgttt aaacgcggct tcggcggcgg
    12841 agagttcggg ggcaaggttg tccagttcgg acgcgatgcc gtcgagacgc gctttttggg
    12901 caatcaggct ttcctgcgcg ggtttggcat agagcaggac gctgactttg tcgacctgat
    12961 ggccttcggg cgtgagccag atttggtgtg cgcccaaatc gctttgatgc gcgagggcgt
    13021 aattcaaatc gggcgcgcac aatacgccgt cgagccagtg gtgcaatgcc gtctgaaacg
    13081 gcggctgcgc ttggatttgg ttcagcaatg cctgtacggg cagggatttt ttgatgccgc
    13141 cggagaggcc gtctgaaagc cacgccgcct gcccctgcgg caaaggcgca ggaggaacga
    13201 agccgtgcgg cacgctgcgg gcgtgcaggc gttcggcaag aatgacggac aaggcgtgct
    13261 gccactcggt gggcgcggtg atgtgttgcc acaattgcgg cgcggcggcg taatcggttg
    13321 cctgccagaa gtcggcggct tcctgctgtt gcgacaggat ttgcgacaac gcctgctgct
    13381 gcgcctgcaa ggtaatgtgt tgctgcttca ggctttggaa gcggtttgag gccgtctgaa
    13441 acgcctcgcg ggcggcgtgt aaggcttctt cggcggcgat gatttgttct tcgtaatgct
    13501 cttgctggct ttgcaacaag gcagcggcct cctgcgcggc ggcggtttcc gcttcgtcgg
    13561 gcaggttcag ggcttggttc tcctgtttca gacggccttt gcgctcttcg tgcttggcaa
    13621 cggtttgttc ggcatgggca agctgctgct gcttcaacgc cagttcgcgg cggatgcggt
    13681 ttgcctcatc ctgctggatt tggaaggcgg cgttgagcgt ggcttgggct tcttccaatt
    13741 cgggcagacg ctcttcgtgt tcggcaacct gcatcgccca ttccgccaat tcggtttgct
    13801 tttcttgggt ctgcaactcg ttttcttcaa gctgaacgcg gatttgctgc tgttcctgat
    13861 ggatgcgctg catctgcgcc tgcgccgcct gcttgtcgcg ttcgatgcgt tggtgcaggt
    13921 tctgctgatg gcggatttgt tcttccaaac gggcaatctg ctcgcgcaac acgccgcgtt
    13981 tgttgctcaa ttcgtgtacc gcctgctgct gcgactgttc ggcggtctgc aaggcgcgta
    14041 cttcgtcgtt taacgcctga acctgcgcgg cggtttcgtc ctgctgcgcc tgcaaagact
    14101 gatgctgcgc ggtcgccttg tcggcggcgg taagcgattg ctgccattgg gcgtaatcga
    14161 gcaaatcctg ctgttggttc aactgcgcgg tcagggattt gtagcgttcg gcggtttccg
    14221 cctgtttctc cagcttttcc acctgacgcg ccaattcgtt ctgcaaatcg cccaaacgct
    14281 gcaaatgctc gcgcgtgtct ttcagacggc cttccgtctc cttgcggcgt tccttatatt
    14341 tggacacacc cgccgcctcc tcgatataag cgcgcaactc ctccggccgc gcttcgatga
    14401 tgcgcgaaat catcccctgc tcgataacgg cataaccgcg cgcgcccacg cccgtaccca
    14461 gaaacaaatc agtaatatcg cggcggcgca cggtctgatt gttgatgaaa taagtcgatt
    14521 cgccctgccg cgtcagctgc cgcttgatgc tcacctcggc atactgcccc cacgccccct
    14581 gcaaactatg gtcgctgttg tcaaacacca gctccaccga agccctcggc gcaggacggc
    14641 gcgtcgccgc accgttaaag atcacgtcct gcatactctc gccgcgaagc tgcttcgccg
    14701 aagcctcgcc caacacccag cgcaccgcgt caatcacatt cgacttgccg cagccgttcg
    14761 gcccgataac cgcaacaagc tgccccggca catgaatcgt ggtcgggtcg gtaaaagatt
    14821 tgaagccgga gagtttgata tgggtcaggc gcataatggt cggaaaaaat aaaaaagaag
    14881 cgtattttaa cggaaatccc gccgcaccac cccatatctt gccggcaaaa ccttaccgca
    14941 tcccgcaccc tcgatgccgt ctgaagcctt cagacggcat ttttaacgcg cccgaacccc
    15001 gattccgcca acgcccgatg cttggcgcgg catttgccgt attgccccgc cgccgggcgc
    15061 gttataatcc gccacgacat ccgcccgatt tggaaacctg cctatgaaac ccgcctattt
    15121 catctccgac ctgcatttga gcgaaaagca ccccgaactg accgcgctgt tgctgcgttt
    15181 tttacgttct tccgccgccg ggcaggcgcg ggcggtttac attttgggcg atttgtttga
    15241 tttttgggtg ggcgatgacg aagtttccga gttgaatact tcggttgcgc gtgaaatcag
    15301 gaaattgtcc gacaaaggcg ttgccgtgtt cttcgtcagg ggcaaccgcg acttcctgat
    15361 tggtcaggat ttttgccggc aggcaggcat gacgctgctg ccggattact cggttttgga
    15421 cttgttcggc tgcaaaaccc tcatctgcca cggcgacact ctgtgtacgg atgacagggc
    15481 ataccaacgt ttccgcaaaa tcgtgcatcg gaagcggctg caaaaactgt tcctgatgct
    15541 gcccctgaag tggcgcacgc gccttgccgc caaaatcagg cgcgtcagca aaatggaaaa
    15601 acaggtcaag cccgccgata ttatggatgt caatgccgcc tttaccgcgc ggcaggttcg
    15661 cgcctttaac gcggaaaggc tgatacacgg acacacccac cgcgagcata tccatcacga
    15721 aaacggcttt acccgcatcg ttttgggcga ctggcataac gactatgctt caatcctccg
    15781 cgtggacggg gacggcgcgg tattcgtgcc gctggaaaat gctgaaaatg ccgtctgaag
    15841 cctttcgggc ggcttttttt acacgcccct ttcccacact ccctccgccg catcatcacc
    15901 atccttaacc cgcattaata gccattatcg ataaaatgct tgaccgatta cataagatta
    15961 cgtaaagtgt gcaaacgcat aacaattggt cttaccaatt tatcttttaa ccaaaatatc
    16021 gggtgtacgg gatttgaacg tctttccaaa cctcgacgcg ccttatgatg caaatgccta
    16081 aaaaggattc gttatggcac ttttcctcag catattcccc atcgtcctgc tgatttggct
    16141 gatggtgaaa aaaaacagta tgccctccta cgtcgcgctg ccgattaccg cagtgctgat
    16201 ttacgccatc aaacttttct acttcggcga tgcgggaatg ctgctcaacg ccaccgccgc
    16261 ttccggcctc gtcaaaacgc tcacaccgat taccgtgatt ttcggcgcga ttatgttcaa
    16321 ccgtatgatg gaaaccacgg gctgcatcga tgtcatccgc aaatggctgg cgaccatcag
    16381 cccgaacccc gtagcgcaac tgatgattat cggctggtcg tttgccttta tgattgaagg
    16441 cgcatccggc ttcggcacgc ctgctgcgat tgccgcgccg attctgatga gcttgggctt
    16501 caatccgttg aaagtagcga ttttcacttt ggtgatgaac tccgtccccg tctctttcgg
    16561 tgcggtcggt acgccgactt ggttcggttt tgcaccgctg aacctgagtg ccgaagacat
    16621 cctcgctatc ggcaggcaga ccggcgtgat gcacttcttc gcaggtttcg tcatccccgt
    16681 tatcggcttg agttttatcg taccttggtc tgaaatccgc aaaaacttgg gcttcgtcgc
    16741 cattgccgtt ttctcctgta cccttcccta tgtcgcattg gcaatggtca acgaagaatt
    16801 tccttctttg gttgccggcg caatcggtct gatgttttcc gtatttgcag ctaataaagg
    16861 ctggggcttg agcaaagacc acgccaaaga cccgaatgcc gaaaaagtgc cgttcgccca
    16921 agtcgccaaa gcactcgccc ctttgggtat gctgatcggc atgctggtgg ttacgcgcat
    16981 caaacagctc ggcatcaaag gcattttgac cagcaaagaa gaatggttca gcttccaact
    17041 gccgtttgat ttgtccaaaa tcaccgtcag cgactccctg acgattacct tcggcaatat
    17101 tttcggacaa gatgtcagcg cgtcttacca aacgctgtac gtcccggctt ggattccgtt
    17161 tgtgctgacc gtttggattt gcatcctgct gtataaaacc aaattcaaag atgcctggac
    17221 gatttattcc gtaactttca atcaaaccaa aaaaccgctg cttgccctga tgggcgcgct
    17281 gattatggtt cagctgatgc tggtcggcgg cgacaattcg atggtgaaaa tcatcggtaa
    17341 ggaatttgcc gcaatggcgg gcgaacactg ggtttatttc tcgccgtatc tgggcgcgat
    17401 cggtgcgttc ttctccggct ccaacaccgt gtccaacctg accttcggac cgattcagca
    17461 gcaaatcgcc ttggataccg gcctgtccgt taccctgatt ctggcgttgc agtccgtcgg
    17521 cggcgcgatg ggcaatatgg tgtgcctcaa caacatcatc gccgtatgta ccgtattgga
    17581 tgtgaaaaat tccgaaggtg cgattatcaa gaaaaccgtt atcccgatgg cgatttacgg
    17641 cgtgattgcc gtcgtcgcgg caatgatttt cttcctctag gcgaaacgcc accatccata
    17701 gcggcagcac gcggggaacg gaaaccgccg ttccccgtcc gggcggcgca tcgggcagaa
    17761 tctcggtgcc ggaacagccc gccccgccaa acaaatgccg tctgaaaccg gaaaaggctt
    17821 cagacggcat ttttccgccg cttgcgttca ggcgtaatga tgatgcggca tcttgccgtc
    17881 aaggcggtag cgcgtgccgc aatacgggca ggcaacgctg cccgattctc cttcgcacaa
    17941 aggcaaaaac actctcggat gcccgttcca ctgctcgttg tcgggtcccg agcaatacag
    18001 cggcagattt tccggcaaca cggaaatttc ctgcagattc agattgtcca tttgattttc
    18061 ctttgcgtgg ttggcgtgcg cccattttac gccatcggca ggctaaagga tattttcggc
    18121 gcaaagccgc aatccgctat aatccccact tttcagacgg cataccatga ctgcgcttac
    18181 ccttccggaa gacatccgcc aacaagagcc atccgccctg ctctataccc tcgtttccgc
    18241 ctacctcgaa cacaccgccc aaaccggcga cgaatccctc tcctgcctgt ccgacgacca
    18301 acacacgctg accgcattct gctacctcga cagccaagtc gaagaaggcg gcttcgtgca
    18361 actcatcgcc tccggttacg gcgaatatat tttccgcaac ccgcttgccg acagcctgcg
    18421 ccgctggaaa atcaaagccg tgccgaaagt cttggacaaa gccaaagccc tctacgaaca
    18481 acacggcaaa accatcgaaa cgctcgccga cggaggcgca gacatcgaat ccctgcgcaa
    18541 acagttcccc gactttgaag aatgggacgg cgcatactac gaagccgccg aacaagacct
    18601 gcccctgctt gcagaacaca tacagtcaaa ctgggaaacc ttcgcccata tcgggcaggc
    18661 gtgattgcgt ctgtttccag cccgtgtaaa acagcgtaaa atcggcaaac ccgaatgacc
    18721 ttccgtcccc atcaaggagc aagctatgtt cttcaagcac atcgaagccg cccccgccga
    18781 tccgattctc ggtttgggcg aagcgttcaa agccgaaacc cgccccgaaa aagtcaacct
    18841 cggcatcggc gtttataaag acgcatccgg cgcgacaccc ctcgtcaaag ccgtcaaaga
    18901 agccgaaaaa cgcctgttgg aaagcgaaac caccaaaaac tacctcacca tcgacggcgt
    18961 tgccgactac aacgcgcaaa cccaaatcct gctgttcggc aaagaccacg aaatcatcgc
    19021 cagccgtcgc gccaaaacag cgcaaagcct cggcggtacg ggcgcattgc gtattgcggc
    19081 cgagtttgcc aaacgccagt tgaacgcgca aaccatctgg atttccaatc cgacttggcc
    19141 caaccacaac gccatcgcca aagcggtcgg tatccaagac aaaccttatc gttactatga
    19201 tgccgccaaa cacggtttgg attgggacgg catgattgag gacttgagcc aagcgcaaaa
    19261 aggcgacatc gtcctgctgc acggctgctg ccacaatcct accggcatcg accctacgcc
    19321 cgaacaatgg gaaactttgg caaaactttc tgccgaaaaa ggctggctgc cgctgtttga
    19381 ctttgcctac caaggcttcg gcaacggttt ggaagaagat gcctacggct tgcgcgtgtt
    19441 cttgaaacac aatacagaat tgctgattgc cagctcttat tccaaaaact tcggtatgta
    19501 caacgagcgc gtcggcgcgt tcactttggt ggccgaagac gaagaaacag caatccgcgc
    19561 acacagccaa gtcaaaacca tcatccgtac cttgtattcc aacccggctt cacacggtgc
    19621 gaacaccatt gcgctggtgt tgaaaaatga tgatttgaaa gcccaatgga ttgccgaact
    19681 cgatgaaatg cgcggccgca tcaaagccat gcgccaaaaa tttgttgcgt tgctcaaagc
    19741 caaaggtgca agccaagact ttgatttcat tatcgaacaa aacggtatgt tctctttcag
    19801 cggtctgact cccgaacaag ttgaccgttt gaaaaacgag tttgccattt atgccgtccg
    19861 ctccggccgc atcaacgttg ccggcattac cgacgacaac atcgattatc tgtgcgaaag
    19921 catcgtgaaa gtactgtaaa aagcacagcc atatgaaaat gccgtctgaa ccttaaccag
    19981 ttcagacggc atctgttttt tgttaaaaaa caataaacca taacaatttc aaacaagcta
    20041 aaaacatcta taaaatcaac ccaatatgat gaactcaaat aaaaaatacg aatatcatca
    20101 ttctcaaatt caaaatttga acgccacagt attatccg
//
LOCUS       DAXIHS010000033        19789 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXIHS010000033 DAXIHS010000000
VERSION     DAXIHS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.033"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..449
                     /locus_tag="ACFOF2_001028"
     CDS             <1..449
                     /locus_tag="ACFOF2_001028"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7107366.1"
                     /translation="LDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYLPYSEMMSIA
                     KGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQTNAEVLSLLNLLPHENYRSGDV
                     DSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKIVNLIERLANE"
     gene            442..969
                     /locus_tag="ACFOF2_001029"
                     /pseudo
     CDS             442..969
                     /locus_tag="ACFOF2_001029"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            1018..2019
                     /locus_tag="ACFOF2_001030"
     CDS             1018..2019
                     /locus_tag="ACFOF2_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC7107367.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            2006..2692
                     /locus_tag="ACFOF2_001031"
     CDS             2006..2692
                     /locus_tag="ACFOF2_001031"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC7107368.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            2740..2934
                     /locus_tag="ACFOF2_001032"
     CDS             2740..2934
                     /locus_tag="ACFOF2_001032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107369.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPQDRDGISD"
     gene            2928..3044
                     /locus_tag="ACFOF2_001033"
                     /pseudo
     CDS             2928..3044
                     /locus_tag="ACFOF2_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3163..4338
                     /locus_tag="ACFOF2_001034"
     CDS             3163..4338
                     /locus_tag="ACFOF2_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC7107370.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVVVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHNLWVVEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLAEATR"
     gene            4386..6296
                     /gene="pglD"
                     /locus_tag="ACFOF2_001035"
     CDS             4386..6296
                     /gene="pglD"
                     /locus_tag="ACFOF2_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC7107371.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDPANWQSFLLTAFLTACLFFRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMAPVIIYGAGRSGRQLLKA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RLRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            6352..7644
                     /locus_tag="ACFOF2_001036"
     CDS             6352..7644
                     /locus_tag="ACFOF2_001036"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC7107372.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDNLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(7696..8532)
                     /locus_tag="ACFOF2_001037"
     CDS             complement(7696..8532)
                     /locus_tag="ACFOF2_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC7107373.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(8978..9181)
                     /locus_tag="ACFOF2_001038"
     CDS             complement(8978..9181)
                     /locus_tag="ACFOF2_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC7107374.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(9159..9392)
                     /locus_tag="ACFOF2_001039"
     CDS             complement(9159..9392)
                     /locus_tag="ACFOF2_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC7107375.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            9930..13364
                     /gene="dnaE"
                     /locus_tag="ACFOF2_001040"
     CDS             9930..13364
                     /gene="dnaE"
                     /locus_tag="ACFOF2_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC7107376.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYQRLSELLT
                     EAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVCQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGSQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            13642..16890
                     /gene="cas9"
                     /locus_tag="ACFOF2_001041"
                     /gene_synonym="csn1"
     CDS             13642..16890
                     /gene="cas9"
                     /locus_tag="ACFOF2_001041"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFC7107377.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVANNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSSELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADKIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLVRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKECNLNDTRYVNRFLCQFVA
                     DHILLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGKVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGAHKDTLRSAKRFVKHN
                     EKISVKRVWLTEIKLADLENMVNYKNGREIELYEALKARLEAYGGNAKQAFDPKDNPF
                     YKKGGQLVKAVRVEKTQESGVLLNKKNAYTIADNGDMVRVDVFCKVDKKGKNQYFIVP
                     IYAWQVAENILPDIDCKGYRIDDSYTFCFSLHKYDLIAFQKDEKSKVEFAYYINCDSS
                     NGRFYLAWHDKGSKEQQFRISTQNLVLIQKYQVNELGKEIRPCRLKKRPPVR"
     gene            16956..17870
                     /gene="cas1"
                     /locus_tag="ACFOF2_001042"
     CDS             16956..17870
                     /gene="cas1"
                     /locus_tag="ACFOF2_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC7107378.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFIADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARTLTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKAELTPGIKQHLIKTLHYQISIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKEYRYE"
     gene            17863..18189
                     /gene="cas2"
                     /locus_tag="ACFOF2_001043"
     CDS             17863..18189
                     /gene="cas2"
                     /locus_tag="ACFOF2_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC7107379.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   18253..19212
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18253..18288
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
     gene            19652..>19789
                     /locus_tag="ACFOF2_001044"
     CDS             19652..>19789
                     /locus_tag="ACFOF2_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC7107380.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YL"
BASE COUNT         5233 a         5465 c         4814 g         4277 t
ORIGIN      
        1 ttttagacga cggcgaaaac gtcgaattgc acattatggg cggaggaccc gatttggaga
       61 aactcaaaca atatgaaaat cgcgccatca agttttacgg ttatttaccc tattctgaaa
      121 tgatgtcgat tgccaaaggc tgcgacattg ccgtcaacgc catccatagc cacgccatgc
      181 agtcggttac caacaaactc tccgactata tggctttgca aaaaccgatt ctgaacagcc
      241 agaccaatgc cgaagtcctt agcctcttga acctgctgcc gcacgaaaac taccgttccg
      301 gcgacgtgga cagcttcgtt caagccgcca aagatatttt gaaacgcaaa aacgaccccg
      361 ttcagtccga tgaaatcgtc cgccgcttca ggcgcgacat ttcctatcgg aaaatcgtca
      421 acctgattga aagattggca aatgagtaaa gccgtcaaac gcctgttcga catcatcgca
      481 tccgcatcgg ggctgattgt cctctcaccc gttttcctga ttttggcgta cctcatccgc
      541 aagaatctgg gttcgcccgt cttcttcatt caagaacgcc ccggaaagga cggaaaacct
      601 tttaaaatgg tcaaattccg ttccatgcgc gacgcgcttg attcagacgg cattccgctg
      661 cccgacagcg agcggctcac acccttcggc aaaaaactgc gtgccgctag tttggacgaa
      721 ctgcctgaat tatggaatgt cttaaaaggc gacatgagcc tggtcggccc gcgcccgctg
      781 ctgatgcaat atctgccgct gtacgacaac ttccaaaacc gccgccacga aatgaaaccc
      841 ggcattaccg gctgggcgca ggtcaacggg cgcaacgcgc tttcgtggga cgaaaaattc
      901 gcctgcgatg tttggtatat cgaccattac agtttttggc tggatatgaa aatcctgttt
      961 ttgacagtca gaaaagtctt tatcaaagaa gggatttcgg cggaagggga agccactatg
     1021 ccttatttca ctggaaatca aactaatgaa aaaaataata tactgattct gtcggcaggt
     1081 cgccgtgtcg aattggtgca ggacttccaa accgaagccg cacgtttttc agacggcatc
     1141 agcgtttttg ccactgacct caacccccgc atgtcttctg cctgccatgt ttcggacggt
     1201 tcatttgaag tgccgccgat tagtaccgat tcatatatcg acagcatttt caatctggcg
     1261 gtacgcgaaa atatcggttt aatcatccca accatagaca ccgaactgca aaagctggca
     1321 gacgagcgcg aacgctttga agcggcaagt gtccacatcg tcgtttccga ttcggacttc
     1381 atcgcacaat gccgcgacaa acggaaaacc gccaccttgt ttgcccgcta cggcatacgc
     1441 tcacccgaaa tttacgaccg caagaagttg gttttccctt gcttcgccaa gccttatgac
     1501 ggcagccgcg ccatcggtgc aaaacggatt gacacacctg ccgacctgac accggaagtc
     1561 ttggcagaca ccaaattaat gttctgtcag tttatcgaca tcgaaaataa attttccgag
     1621 tttaccgtcg atatgtatta cgaccgtcaa tgccgtctga aatgcgccat cccgcgaaaa
     1681 cgcttggaag tgcgctccgg cgaaatcagc aaaggcgtta cctgcaaaaa cagcctctac
     1741 caaacgctgc tggaaaaaat gtcggtattg cagggcgcgc gcggctgtat cacggcgcag
     1801 ttcttctaca acgaagaaac cggcgaattc tacggcgtgg aaatcaaccc ccgcttcggc
     1861 ggtggcttcc cgctgacgta tgccgcaggc ggcaattatc cgagccggtt gatgcgcgaa
     1921 tacctcggcg acgaagaaat cccgttttca gacgactggg aaaacaacct cattatgttg
     1981 cgctacgatg ccaaggtctt ggttcatgaa aacgattaat cctgaaacca ccgtcatcgt
     2041 cttcgacttg gacgataccc tgtattcgga atacgaatac aagctctccg gtatccgttc
     2101 ggtcatcgat accgttgccg cactgtatcc cgattggaat tcagacaacc tatggcgcaa
     2161 catcgacccc gacggcaaag attggctgga caaattgtgc cgccactgtg gttttaatga
     2221 atcggaaaaa caggttcttt tgtggcaata ccggctgcac cgcccgacgc tgactccgta
     2281 tgctccgccg gatttcctgt ccgaactgac cgcgcccttc gccgcacgcg cgctgattac
     2341 cgacggcaga agcctgaccc aacgcctgaa gctggaagcc ttgggtctgt cttccctgtt
     2401 tgacgacatc ctcatctctg aagcctgctc ttcggaaaag cccgacggca aacgcttccg
     2461 ccatcttcaa gacaaatatg ccgacaaggc aggctgcttc atctatatcg gcgacaacat
     2521 ttccaaagac tttatcgcgc cgaacacttt gggctggata accataggat tgcttccctc
     2581 cggacagaac atccaccgcc accagcctga aagttttaac acagaatatc atcccgactt
     2641 ttgggttaac tcccttcaag atttagttcc tataattgat tcaaatactt aaccattaca
     2701 taatcaaaca tgcactcaca atattaaaag gactatttca tgacaaaata ttattcatgg
     2761 ggcgtaaacg gctttccgcc ctacgttttc gacaccattt ggaaacgatg gaagttgaat
     2821 gggcgcgggg gcggttatcg tgtgcgacat cccggacggc atgaccgtcg cgggcaatcc
     2881 ggcaaagccg ctgccgcgca aaaaccccaa gaccgggacg gcataagcga ttaaaatata
     2941 gtggattaaa tttaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacggc
     3001 aaggcgaggc aacgccgtac tggtttaaat ttaatccact atacaccccc gtacagaccg
     3061 attttgacaa cacctgcggc gcgcgcccga ttcttcggaa cacgcccccc ttcagacggc
     3121 atagggtcgg aaatgccgtc tgaacccgac ggaccaaaca ccatgctgaa cacctccctt
     3181 tccccgtggc cctgcttcac ccaagaagaa gccgatgccg tttccaaagt cctcttgtcc
     3241 aacaaagtca attactggac gggcagcgaa tgccgcgaat ttgaaaaaga attcgccgcc
     3301 ttcgccggca cgcggtacgc tgtcgcccta tccaacggca cgctggcact cgatgccgcg
     3361 ctcaaagcaa tcggcatagg cgcgggcgat gatgtggttg ttacctcgcg caccttcctc
     3421 gcctccgcgt cctgcatcgt gaacgcgggc gcaaaccccg tgtttgccga cgtggatttg
     3481 aacagccaaa acatcagcgc ggaaaccgtc aaagccgtgc tgaccccgaa taccaaggcg
     3541 attatcgtgg ttcaccttgc cggtatgcct gccgaaatgg atggcattat ggctttggca
     3601 aaagaacata atctttgggt tgtcgaagac tgcgcccaag cgcacggcgc gaaatacaaa
     3661 ggcaaatccg tcggcagcat cggacacgtc ggcgcatggt cgttctgcca agacaaaatc
     3721 atgaccacgg gcggcgaagg cggcatggtt acgaccaacg acaaaaccct gtgggaaaaa
     3781 atgtggtcgt acaaagacca cggcaaaagc tacgatgccg tgtaccaccg cgaacacgcg
     3841 cccggtttcc gctggctgca cgaaagtttc ggcacaaact ggcgtatgat ggaaatgcag
     3901 gcggcaatcg ggcgcatcca gctcaaacgc ctgcccgaat ggactgcctg ccgtcaagcg
     3961 cacgccgcca agctggcgga aagcctaggc aaattcaaaa gtatccgctt ggttgaagtc
     4021 gccgactaca tcggacacgc gcaatataag ttctacgctt tcgtcaaacc cgaacacctc
     4081 aaagacggct ggacgcgcga ccgcatcgtc agcgaactga acgcgcgcaa agtcccctgc
     4141 tatcaaggca gctgctccga agtctatttg gaaaaatctt tcgacgacac gccttggcgg
     4201 ccgaaagagc ggctgaaaaa tgctgtcgag ttgggcgaca ccagcctgat gttcttagta
     4261 catccgacgc tgaccgacgg cgaaatcgcg ttctgcaaag aacacatcga agccgtattg
     4321 gcagaagcca cacgataacc attcagacgg catatgccgt ctgaaaaata cacgccgccg
     4381 acaacatgac tctggaaacc ctgatcgccc tgccgcgcaa catcaagaaa atctgtttcc
     4441 tcatacacga ttttctgatg attttcattg ccttttggtt tacccaaagc ctgaaggccg
     4501 actactcgga cgaatggttc gaccccgcca actggcagtc tttcctattg accgcgtttt
     4561 tgaccgcctg cctgttcttc agaatggggc tttaccgcgc cgtcacacgc ttcatcagct
     4621 tccgcatcct ctccaccgcg ctggcaggca gcctcgcctc cgccgtattg tttttcctca
     4681 atacgctgat atttgaagaa cggctgcggc tcgccctgcc gattgtctat ttcttactgc
     4741 tgtttgtttc cgtgaccggc tcgcgtatgg ttttgcgcgg actgttgtcc gaacacccca
     4801 aaaaacagat ggcccccgtc attatttacg gcgcgggacg gtcgggcaga caactgctta
     4861 aggccgtcaa acaaatgcgc gaatattccg ccgccgcctt tgtggacgac gaccccaaac
     4921 tgtggcacac cgtcatctac gaccttgccg tttaccagcc cgatgccatc gccttcctca
     4981 tcgaacgcta cggcgtggaa aaaatcctgc tcgccatccc cggcgcgact caggaacaac
     5041 gcctccgaat catcaacaaa ctggaagcct atccctgcga agtgttgacc attcccggaa
     5101 tgaaagacct gatggacgga aaaatcagca tcggcacgct caaaaaaatc tctgtgtccg
     5161 acctgctcgg gcgtgattcc gtcgcaccag acgaccgcct catgaatgcc gacatcgaag
     5221 gcaaaaccgt catggtaacc ggcgcgggcg gctccatcgg ttcggaactc tgccgccaga
     5281 ttatccgccg ccgccccgaa aagctgctgc tgttcgagtt atccgaattc gccctgtacg
     5341 ccatcgaaaa agaattgcgc gaaacctgca tccaaaaacg cctcgatacc gaaatcctgc
     5401 cctttctcgg ttcggtgcaa aaccgcacgc tgctcgaaca cgtcatgacc gccttttccg
     5461 ttgcgaccgt ctatcacgcc gccgcctaca aacacgtccc catggtcgag ttcaacaccg
     5521 tcgaaggcat acgcaacaac atcttcggca cactcgagtg cgcgcttgcc gccacggcat
     5581 cgggcgtaag aactttcgtc ctcatctcca ccgacaaagc cgtccgcccc accaacacca
     5641 tgggtgccgg caaacgcatg gcggaactct gccttcaagc gctcgccgcc gaacccggac
     5701 aaaaaacccg cttcagcatg gtgcgtttcg gcaatgtttt aggttcgtcc ggctccgttg
     5761 tcccgctgtt tgaaaaacag attgcagaag gcggcccgct taccctgacc caccccgaaa
     5821 tcacacgtta tttcatgacc atacccgaag ccgcccaact cgtcatacag gcaggcgcga
     5881 tgggtacggg cggcgacgta ttcgtcctcg acatgggtga atccgtcaaa atcatcgacc
     5941 ttgcccgcca aatgattacc ctaagcggcc tcaaacccaa cacacccgaa caacccgacg
     6001 gcgacatcga aatcctcatt accggactgc gtcccggaga aaaactctac gaagagctgc
     6061 tcatcggcga caacgtccgc aaaaccggcc atccgcgcat tatgacggct gacgaggcga
     6121 tgctgccgtg gcacgagctc tccgccctgc tcgaccgcat ccgtgcggcc tgcgaccgtt
     6181 acgaccagca ggcaatccgc accctgctca tcaacgcccc gaccggcttt gccccgagcg
     6241 acggcatctg cgacctgctt tgggtacgag aaacacacag aaaaaatgcc gtctgaacct
     6301 tcagacggca taacgtacaa accaacctac cttacacaca cggagtttga catgcagttt
     6361 tcagcattcg gcgaaaaatt cacgcaacac agcggcatcc tccaactaat ggacaacctc
     6421 ggcgacgcgc tcaaaagcga caagcccgtc aacatgctcg gcggcggcaa tccggcgcgc
     6481 attccggaaa tcgatcgggc gtttgccgac atattttcca aactggcggc agaacacgcc
     6541 gtcgaaaaca tcggcaacta ctccaatccc caaggcgatg ccgtgctgat tgacgcgctg
     6601 accgccttcc tcaaccgcga atacagctgg aatctgaccg ccgacaatat cgcgctgacc
     6661 aacggttcgc aaaacgcgtt tttctatctt ttcaacctct tcggcggcaa attcaagctt
     6721 tcagacggca catccgcaga aaaagccatt ttgttgccgc tcgcgcccga atacatcggc
     6781 tatgccgacg tgcatgtcga agggcagcac ttcgtttccg tcaagcccaa aatcgaaaac
     6841 gtcgaacacg aaggcgaagc aggcttcttc aaataccgcg tggactttga cgcactggaa
     6901 aacctgcccg aactcaaagc gggcaaaatc ggcgcgattt gctgttcgcg cccgaccaac
     6961 ccgaccggca atgtgttgac cgacagcgaa atggcgcgtt tggacgcttt ggcgcgtgaa
     7021 cacgggattc cgctgattat cgacaacgcc tacggaatgc cgttccccaa catcatttac
     7081 agcgacgtaa cgctgaattg gcatgaaaac atcatcctct gcttcagcct ttccaaagtc
     7141 ggcctgccgg gcgttcgcac cggcatcatc gtcgccgcgc cggaagtggt caaagccgtc
     7201 agcagcctga acgcgattgt gaaccttgcc cccacgcgct tcggcgcggc catcgccgcc
     7261 ccgctactgg aaagcggcga gatgaaacgg cttgccgacc aagtcatccg gccgttttac
     7321 cgcaatcagg cgcaaaccgc cgtctcgctg ctcaagcgcg agctgggcgc gtacccgatg
     7381 aaaatccaca aacccgaagg cgcgattttc ctgtggctct ggtttgaaaa cctgcccgtt
     7441 tcttcgcaaa ccctgtacga aatgctcaaa gccgaaggta cactgattat tccgggcgaa
     7501 catttcttcg tcggcatcga cacgcaggat tacccgcacg cgggcgagtg catccgcatg
     7561 agcatcgcgc aggacgctca aacgctggaa aaaggcattg ccgccatcgg caaaaccgtc
     7621 cgaaaactgt acgacaacgt ttaaaccgca aaaaatgccg tctgaaaagt tttcagacgg
     7681 catttttatc tgcattcaat gtcgggaaaa atgcgcccac actggcctgc agttttccgg
     7741 cagttcggga cacgcgccga ggatgccgcc gctgaagtcg tttaaacggt ggaagtcgct
     7801 gcccgcgctt gccaacaatc cgaagcgttc tgccaaaaga gcgtagttga ggcggtcgtt
     7861 tttgcaacag ttgccgctgt ggacttcgat gcctgcgccg ccgaggtttt taaattcttc
     7921 aaacagattg cgcttggcgg tggcggacaa atcgtagcgc atcggatggg cgatgaccgc
     7981 cagcccgccc gcgccgttga cggcggagac gcagtcttcc agcgtcgccc attcgtgtcg
     8041 gacggcgcag gatttgccgt cgcccaagta tttggtgaac gcctgctgct tgtttttgac
     8101 atgtcccgcc cggatgagga actcggcgac gtgggtgcgg ctgaccattt ctttgtttgc
     8161 cgccagcgcg agcgcgccgt cgtatgcgcc cgaaatgccg cgtttttcca gcttggcggc
     8221 gatggcttcg aggcgtttca gacggccttg ccgcacttgc gccaacaggt tttgcaggtt
     8281 ttcgtcttgt tcgtcaaagt ctaagccgac aacgtgtatg gtacgcccgc gccacgttac
     8341 ggagatttcc acgccgttaa tcaggcgcaa accgagcctg tcggcttcgg cacgcgcttc
     8401 ggcgatgccg ccggtgtggt cgtggtcggt caacgccagc agcgtgcagc cgttttgatg
     8461 cgcgaggcgc acaacttcgg cgggggagag cataccgtcg gaaacggtgg aatggcagtg
     8521 caggtctatc ataaggcgtt atgtgcgtgg taaatggtgg gcagggtgtt tcaatattgt
     8581 cgggcggtgt ggcggcggtg atttcaaaaa acaggtgtat ggcaaatgca aaggaaaagc
     8641 cgctatgccg tcattccagc gcaggcggga atccagactt tgatttattg gaaatgttta
     8701 aggttaaccg ctatttcaaa cttccggatt cctgctttcg agggaatggc gatatgaccg
     8761 ttttcaattt taatctacta taaaagactg tctgaaaacg tggttttata gtgaattaaa
     8821 tttaaaccgg tacagcgttg gctcgccttg gctcaaagag aacgattctc taaggtgctg
     8881 aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggctcgcc gccttgtcct
     8941 gatttttgtt aattcactat atcaagccga accgttttca gacggcatcg tccgaccaac
     9001 ccgcttcttt caatttccgc cgttgcgcgt cgtagcgggc cttatccgcc caataaatcg
     9061 tctgaataca cgcctcgccg cagtcgctgc cgcagcattc ccacgactcg ggtcggacgg
     9121 gttcgtctaa aagcggctcg cccaaaaggg cttcggcttt atgcttcagg gtcatatcca
     9181 tcggcgattt ccaagcgcgc accgtcaaac tcgatgactt cgccgccgcg tattttggcg
     9241 gttttacggg tttcggtttc gccgttgcgc gacaccaacc cttcggcaat caacgccttc
     9301 gcctgtccgc cgctttcggc aagtccggcc aatttcaaga ggtcgcacaa ggcgatgtat
     9361 tcgttgtctt cgagatagac gatggcttcc atgatgtccc cttgcagaaa gagaccgtta
     9421 ttgtagcacc tgccgccgcc gtacccaaaa ttaccgcaaa accagcgatg tatccgcacc
     9481 gcctgttccg taaaagtaaa aatgccgtct gaaaccccgt atgccgcaat ccgttcaaag
     9541 aaatcctgcc caacggcaga ctgcaaatcc tgttccccga cgaatccgca ttgacgctga
     9601 tgcacatcct caaacacgaa ctgcccgata caccggcaat cggcatcaaa acgaaatcaa
     9661 aaacctgttt gaacgtaaaa ttaatttaac tgcttgccat ccgtccgaat aaggtatata
     9721 gttccttata actagtttga tagtccttta tatctatcaa tactccttgg gaagcctcca
     9781 ccacacggta ggaggcattt ttttgccgtc gtaaaagctc aaaaacattt gcaggtcatg
     9841 ccgtctgaat ctgaaacggc atgacctaca ccgccatcta aagacaaccc tgctacaata
     9901 cgccttttat tgttcacgcc gattttgcca tgaccgagcc gacctacatc cccctgcgcc
     9961 tgcataccga gttctcaatt accgacggta tggtgcggat taaaaaactg attgccaaag
    10021 cgcaggaata cggtttgcct gctttgggca tcagcgattt gatgaacgaa ttcggtttgg
    10081 tgaaattcta taaagcctgc cgcagtgcgg ggattaagcc tgtcggggcg gcggacgtgt
    10141 ggataggcaa tccgaatgcg cccgacaagc cgttccgcgc catgttgatt atccgcaacg
    10201 atgcgggcta tcagcgtttg agtgagcttt tgacagaggc ctatgtcggt aaagaccgca
    10261 atgtccatca tgcggaactc aaccccgaat ggctggaaaa cggcgacaac agcggcttga
    10321 tttgtttgag cggcgcgcat tacggcgaag tgggcatgaa tctgttgaac ggcaatgaag
    10381 acgcggcgcg cgcggcggcg ttgaaatatg cggcatggtt tcccgatgcg ttttatttag
    10441 agctgcaacg cctgcccgaa cgccccgaat gggaggcttg cgtttccggc agcgtgaagc
    10501 tggcggagga actgggtttg ccggtggtgg cgacgcatcc gacgcagttt atgaaccgcg
    10561 acgatttcaa cgcgcacgag gcgcgggtgt gtatcgcggg cggctgggta ttgacggaca
    10621 aaaaacgtcc gcgcgatttc acgccggacc agtttttcat tccgccggaa acgatgctgg
    10681 agcgtttcgc cgacttgccc gaagccttgg aaaacacggt ggaaatcgcc aaacgctgca
    10741 acctgcacat cacattgggc aaaaacttcc tgcccctttt ccccacgccc gacggtttat
    10801 cgcgcgatga ctatctcatc aaactctcaa acaagggctt gcaggaacgc atggttcagc
    10861 tttatcccga cgaggcggag cgtgcggcaa aaatgccgga atatcaggaa cgtttggatt
    10921 ttgagctgaa catcatcatc cagatgaaat tccccggcta tttccttatc gtacaagact
    10981 ttatcaactg ggcgaaaacg cacggctgtc cggtcgggcc gggacgcggt tcgggcgcgg
    11041 gttcgctggt ggcgtattcg ctgaaaatta ccgacctcga cccgctcaaa tacgcgctgc
    11101 tgttcgaacg tttcttgaac cctgaacgcg tttccatgcc cgacttcgac gtggacttct
    11161 gtcaggcaaa ccgcggccgc gtgattgaat atgtacgcga aaaatacggt gcggaagcag
    11221 tcagccagat tgttaccttc ggcacgatgt cgtccaaagc agtgattcgc gacgtggggc
    11281 gcgtactgga actgccgttt atgctgtgcg acaaactgtc caagctgatt ccgttggaag
    11341 ccaacaaacc tttgggtttg gacgacgcga tgaaggcgga gccgcagatt caggaattaa
    11401 tcgaagcgga agaagcggac gaactgatta cgctggcgaa aaagctggaa gatttgacgc
    11461 gcggtttggg tatgcacgcg ggcggcgtgt tgattgcgcc gggcaagatt tccgattaca
    11521 gccccgtgta tcaggcggac gaatccgcct cgcccgtatc catgtacgac aagggcgacg
    11581 tggaagacgt gggtttggtg aaattcgact ttttaggtct gcgcaacctg accatcatcg
    11641 aaatggcgca gaacaacatc aaaaacacca ccggcgacat catcgatgtc ggcaaaattc
    11701 cgcttgacga ccaggttgcc taccaaatct tccgcgatgc gaacaccacc gccgtgttcc
    11761 agttcgagtc gacaggcatg aagaaaatgc tgaaaacagc gcatacgacc aagtttgaag
    11821 aacttatcgc cttcgtatcg ctctaccgac ccggcccgat ggacaatatt cccgacttcg
    11881 tcgcgcgtat gaaaggtcag gaattccaat acatccaccc gctactcgaa ggcatcctcg
    11941 caccaaccta cgggattatg gtgtatcagg aacaagtgat gcaggcggcg cagattatcg
    12001 gcggctactc gctcggcggc gcggacctgc tgcgccgcgc gatgggtaag aaaaagcccg
    12061 aagaaatggt gaaacaccgc gaaatcttcg ccgaaggcgc ggcaaaacaa ggcatttcgc
    12121 gcgaaaaatc cgacgaaatc ttcaactata tggaaaaatt cgccggctac ggtttcaaca
    12181 aatcccacgc cgccgcctac gccctgattt cctaccagac cgcatggctc aaggcgcact
    12241 accccgccga atttatggcg gcgaccatgt cgtccgaatt ggacaacacc gaccagctca
    12301 agcatttcta cgacgactgc cgcgccaacg gcatcgagtt cctgccgccc gacatcaacg
    12361 aatccgacta ccgctttacg ccgtatccgg acatgaaaat ccgctacgcg ctcggcgcga
    12421 ttaaaggcac gggcgaagcc gccgtcgaat ccatcaccgc cgcgcggcaa agcggcggca
    12481 agtttaccgg cctgttggac ttctgcgagc gcgtcggcaa agaacacatg aaccgccgca
    12541 ccctcgaagc cctgatacgc ggcggcgcgt tcgacagtat cgaacccaac cgcgccatgc
    12601 tcttggcgaa catcgacctc gctatggaca acgccgacca aaaagccgcc aacgccaatc
    12661 agggcgggct gttcgacatg atggaagacg ccatcgaacc ggtgcggctc atcgacctgc
    12721 cgaggtggag cgaatcggaa aaactcgccg aagaaaaaac cgtcatcggc ttttacctgt
    12781 ccggccaccc gttcggcccg tatgcccaag aagtctgcca aatcgcaccg accaaattag
    12841 accgtctgaa gccgcaagac agcgtgcgcc tcgccggatt cgttaccgcc gtgcgtacga
    12901 tgatgggcaa gcgcggcaaa atcgccttcg tcagccttga agatttgagc gggcaggttg
    12961 aaatcatggt cggcagtcag acgctggaaa actgcgccga ctgcctcaaa gccgaccaag
    13021 tgctgattat cgaatccaaa gtcagccgcg acgactacgg cggcggcgac ggactgcgca
    13081 tcatggcaaa ccaagtcatg accctgcaaa cggcacgcga acgctacgcc cgcagcctca
    13141 gcctcgccct cgccccgcat cacgacatcg gcggactggt acggctgctc tcctcccacc
    13201 aactgcccga cacgccgcgc atcccgctgc aactgtcgta tgccaacgaa aaagcgtcgg
    13261 gcaggcttca agtgccgccg aaatggacgg ttacaccgag ctccgcattg ttcggcgaac
    13321 tggaaacatt gctcggcagc cggtcggtgc gcgtcaactg gtaacccaaa atataaatgc
    13381 cgtctgaagc ccaaaatcgg tttcagacgg cattttttaa ccgaaataaa cgatgcccct
    13441 taaagcagaa gctttaaggg gcagagcgtt gcggcacatc ttttcagacg gccttattgt
    13501 agcaacggtt ctcatttcgc agtgcgacaa tatgtttggg attctagccg ttgtgagatg
    13561 ataagtcaat aatctatttg tatataaata agacaataag atatattatc attatttgca
    13621 acgttgttta cggagtaata aatggctgcc ttcaaaccta atccaatcaa ctacatcctc
    13681 ggcctcgata tcggcatcgc atccgtcggc tgggcgatgg tagaaattga cgaagaagaa
    13741 aaccccatcc gcctgattga tttgggcgtg cgcgtatttg agcgtgccga agtaccgaaa
    13801 acaggcgact cccttgccat ggcaaggcgt ttggcgcgca gtgtccgccg cctgacccgc
    13861 cgtcgcgccc atcgcctgct tcgggcccgc cgcctattga aacgcgaagg cgtattacaa
    13921 gccgctgatt ttgacgaaaa cggcttgatc aaatccttac cgaatacacc atggcaactt
    13981 cgcgcagccg cattagaccg caaactgacg cctttagagt ggtcggcagt cttgttgcat
    14041 ttaatcaaac accgcggtta tttgtcgcaa agaaaaaacg agggcgaaac tgccgataaa
    14101 gagcttggcg ctttgcttaa aggcgtggcc aacaatgccc atgccttaca gacaggcgat
    14161 ttccgcacac cggccgaatt ggctttaaat aaatttgaga aagaaagcgg ccatatccgc
    14221 aatcagcgcg gcgattattc gcatacgttc agccgcaaag atttacaggc ggagctgatt
    14281 ttgctgtttg aaaaacaaaa agaatttggc aatccgcatg tttcaggcgg ccttaaagaa
    14341 ggtattgaaa ccctactgat gacgcaacgc cctgccctgt ccggcgatgc cgttcaaaaa
    14401 atgttggggc attgcacctt cgaaccggca gagccgaaag ccgctaaaaa cacctacaca
    14461 gccgaacgtt tcatctggct gaccaagctg aacaacctgc gtattttaga gcaaggcagc
    14521 gagcggccat tgaccgatac cgaacgcgcc acgcttatgg acgagccata cagaaaatcc
    14581 aaactgactt acgcacaagc ccgtaagctg ctgggtttag aagataccgc ctttttcaaa
    14641 ggcttgcgct atggtaaaga caatgccgaa gcctcaacat tgatggaaat gaaggcctac
    14701 catgccatca gccgtgcact ggaaaaagaa ggattgaaag ataaaaaatc cccattaaac
    14761 ctttcttccg aattacaaga tgaaatcggc acggcattct ccctgttcaa aaccgatgaa
    14821 gacattacag gccgtctgaa agaccgtgtt cagcccgaaa tcttagaagc gctgttgaaa
    14881 cacatcagct tcgataagtt cgtccaaatt tccttgaaag cattgcgccg aattgtgcct
    14941 ctaatggagc aaggcaaacg ttacgatgaa gcctgcgccg aaatctacgg agaccattac
    15001 ggcaagaaaa atacggaaga aaaaatttat ctgccgccca tccctgccga caagatccgc
    15061 aaccccgtcg tcttgcgcgc cttatctcaa gcacgtaagg tcattaacgg cgtggtacgc
    15121 cgttacggct ccccagctcg tatccatatt gaaacggcaa gggaagtagg taaatcgttt
    15181 aaagaccgca aagaaatcga aaaacgccaa gaagaaaacc gcaaagaccg ggaaaaagcc
    15241 gccgccaaat tccgagagta tttccccaat tttgtcggcg aacccaaatc aaaagatatt
    15301 ctgaaactgc gcctgtacga gcaacaacac ggcaaatgcc tgtattcggg caaagaaatc
    15361 aacttagtcc gtctgaacga aaaaggctat gtcgaaatcg accatgccct gccgttttcg
    15421 cgcacatggg acgacagttt caacaataaa gtgctggtat tgggcagcga aaaccaaaac
    15481 aaaggcaatc aaacccctta cgaatacttc aacggcaaag acaacagccg cgaatggcag
    15541 gaatttaaag cgcgtgtcga aaccagccgt ttcccgcgca gtaaaaaaca acggattctg
    15601 ctgcaaaaat tcgatgaaga cggctttaaa gaatgcaatc tgaacgacac gcgctacgtc
    15661 aaccgtttcc tgtgccaatt tgttgccgac catatattgc tgacaggtaa agggaaaaga
    15721 cgtgtctttg cctcaaacgg acaaattacc aatctgttgc gcggcttttg gggattgcgc
    15781 aaagtgcgtg cggaaaacga ccgccatcac gccttggacg ctgtagtcgt tgcctgctcg
    15841 accgttgcca tgcagcagaa aattacccgt tttgtacgct ataaagagat gaacgcgttt
    15901 gacggtaaaa ccatagacaa agaaacagga aaagtgctgc atcaaaaaac acacttccca
    15961 caaccttggg aatttttcgc acaagaagtc atgattcgcg tcttcggcaa accggacggc
    16021 aaacccgaat tcgaagaagc cgatacccca gaaaaactgc gcacgttgct tgccgaaaaa
    16081 ttatcatctc gccccgaagc cgtacacgaa tacgttacgc cactgtttgt ttcacgcgcg
    16141 cccaatcgga agatgagcgg tgcacataaa gatactttga gatctgctaa acgatttgtt
    16201 aaacataatg aaaaaattag tgttaaacga gtatggttaa ccgaaatcaa gttggccgac
    16261 cttgaaaata tggttaatta taaaaatggt agagagattg aattatatga ggctcttaag
    16321 gcgcgtttag aggcatatgg aggtaatgct aaacaagcat ttgaccctaa ggacaatccg
    16381 ttttataaaa agggaggaca actggttaaa gctgtgaggg tcgaaaaaac ccaagagagc
    16441 ggagtcttat taaataaaaa aaatgcttat accattgcag ataatggaga catggtacgt
    16501 gttgatgtgt tctgtaaagt agataagaaa ggaaaaaatc agtattttat tgttcccatc
    16561 tatgcttggc aggttgctga aaacattctt cccgatattg attgtaaggg ataccggatt
    16621 gatgatagct atacattctg ttttagcttg cataagtatg atctgattgc ttttcaaaaa
    16681 gatgaaaaat ctaaagtaga attcgcctac tatatcaact gtgatagctc taatggacga
    16741 ttctatttag cttggcatga taaaggctct aaagaacagc aattccgtat tagcacccaa
    16801 aatcttgtat tgatacaaaa ataccaagtt aacgaactgg gcaaagaaat cagaccatgc
    16861 cgtctgaaaa aacgcccacc tgtccgttaa attaatctgt ccctgcttca gacggcctga
    16921 agcagggatt ttttatatca aaataagggg aaaacatgac ttggcgcagc ctacttattc
    16981 aaaacggcgg caaactatcc ttgcaacgcc ggcaactgct gattcaacaa aacggcgagt
    17041 cccataccgt accattagaa gacatcgccg ttatcatcat cgaaaatcgc gaaaccctga
    17101 ttaccgcccc gctcctatca gccttagctg aacatggcgc aaccctgctg acctgcgacg
    17161 agcagttcct gccctgcgga caatggctgc cttatgccca atatcaccgc cagcttaaaa
    17221 tcctcaagct gcaactgaac ataagcgaac ccttaaaaaa gcaattctgg caacatatcg
    17281 tccgccaaaa aatcctgaac caagcattta tcgcagacga aacaggcaat gatcttgcag
    17341 ccaaacggct gcgcactttg gcatctgaag tccgttcggg cgatacagga aaccgtgaag
    17401 cccaagctgc cgccctctac ttccaagccc tctttggcga aaaatttacc cgtaatgaca
    17461 acaatgccgt caatgccgca ttgaactaca cctacgccgt gctacgggcc gcagttgcac
    17521 gcaccctgac cttatacggc tggctgcctg cattaggctt gtttcataga agcgaactca
    17581 atccattcaa tctcgccgat gactttatcg aacccttacg cccattagcc gacctgaccg
    17641 tgatacattt gtacgaacaa ggccgtctga aagccgaact gacccccggc atcaaacaac
    17701 accttatcaa aacccttcac tatcaaatca gcatcgaacg gcaacatttc agcaccttgg
    17761 ctgccatcga caaaatggtt tcgtcatttc aagccggtgt aaccgataaa aacgccaaac
    17821 aactgaaact gcctgaaatc ctaccattga aagaataccg atatgagtga ggccaaattt
    17881 atgaggatta tcgtcttctt cgacctaccg gttatcacgg cggcaaagcg caaagccgcc
    17941 aatcaattcc gccagttttt attaaaagac ggataccaaa tgctccagct ttccgtatac
    18001 agccgtatcg tcaaaggccg cgattcgttg caaaaacacc acaatagact atgtgcaaac
    18061 cttccgcaag aaggctcaat ccgctgtttg gagataacag aaaagcaata tgccgccatg
    18121 aaactgctgt tgggcgagct gaaaacccaa gaaaaaaagg tcaattcaga ccaattattg
    18181 ttattttaag cccatttttt cagactaaat aaaacgggaa atccttatag aataaggatt
    18241 tcccgttggg gtattgtagc actgcgaaat gagaatggga gctacaacaa aagtttatga
    18301 cttgtacgaa tccgcagaat tgtagcactg cgaaatgaga atgggagcta caacccctga
    18361 ttgcaggtga agatgaactg tatgattgta gcactgcgaa atgagaatgg gagctacaac
    18421 aaaatcgtcg gcctgctcca gcacgacctg attgtagcac tgcgaaatga gaatgggagc
    18481 tacaacctac ccgctcggcg tatatgaggc aatctaattg tagcactgcg aaatgagaat
    18541 gggagctaca acgcgcacga ttgacggcag cagacgagcc aaattgtagc actgcgaaat
    18601 gagaatggga gctacaacct cgaatggcga gtacttgacg ctcaatacat tgtagcactg
    18661 cgaaatgaga atgggagcta caaccaggtg cttgggcgta attttatccg ttggattgta
    18721 gcactgcgaa atgagaatgg gagctacaac ccttttgcaa caatcgtgct ttatttgtca
    18781 attgtagcac tgcgaaatga gaatgggagc tacaacttcc tgtcggcttt ttcggctgct
    18841 ttgcggattg tagcactgcg aaatgagaat gggagctaca actatgcttg ggtttgagtg
    18901 ggcgattgat gaattgtagc actgcgaaat gagaatggga gctacaacct tggcacgaga
    18961 tggtcgccgt attaaattat tgtagcactg cgaaatgaga atgggagcta caactttatg
    19021 gctattcacc tgtcgagcaa atcaattgta gcactgcgaa atgagaatgg gagctacaac
    19081 aagaagattg ggaaatgtat ctcacggaag attgtagcac tgcgaaatga gaatgggagc
    19141 tacaaccggt ttgaacagca gtatatggga agaaacattg tagcactgcg aaatgagaat
    19201 gggcgctaca accagccgtt gcgataagcg aacgtgcgtt aaagtcattg agacacagtg
    19261 caattatggc aagaaaggcg acagatattg tgtcacagat ttgaaataag aaaaaaccgc
    19321 ccactcacag agtaggcgga aaacttgcgt ttttttgatt gtggtcgggt tagctactgc
    19381 attgacttaa cagcaaagca accgctaaaa tgaacttgtg gttcacttca acggacagca
    19441 gcaacttttt cccacgcata aggaatttaa gcattttagc ttctcctagt gaagttaatc
    19501 acacaggcga gagccgaaca aaaatccccg ttgcagcggg gatttttgct gttccctgtt
    19561 tgatgattaa actaaaatct tgcaaaatga gacctttgca aaattcccca aaatccccta
    19621 aattcccacc aagacattta ggggatttct catgagcacc ttctttcagc aaaccgcaca
    19681 agccatgatt gccaaacaca tcgaccgttt cccgctattg aagttggacc aggtgattga
    19741 ttggcaacca atcgaacaat acctgaaccg tcaaaaaaac cgttacctt
//
LOCUS       DAXIHS010000034        18850 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXIHS010000034 DAXIHS010000000
VERSION     DAXIHS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.034"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            25..1194
                     /locus_tag="ACFOF2_001045"
     CDS             25..1194
                     /locus_tag="ACFOF2_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC7107381.1"
                     /translation="MNKKRVLLIASYDSFLNTGYAVAKEINYSTIDIYIHQTKKNILS
                     ESQLSLNGLSLKEINFFHIDDYFDKLMYQNYDIVILSLGNGLLTRFFRNSSKLNLTLP
                     IVITLFPGVIFGDSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPIL
                     RKKHHCSDGNKVYFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDIT
                     VHEDKYSYAELAKNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGV
                     ISDLGVDKKYANTHFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLL
                     EVTSTQPPSFDNYFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1242..2105
                     /gene="galU"
                     /locus_tag="ACFOF2_001046"
     CDS             1242..2105
                     /gene="galU"
                     /locus_tag="ACFOF2_001046"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC7107382.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2188..3204
                     /gene="galE"
                     /locus_tag="ACFOF2_001047"
     CDS             2188..3204
                     /gene="galE"
                     /locus_tag="ACFOF2_001047"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC7107383.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            3300..4361
                     /gene="rffG"
                     /locus_tag="ACFOF2_001048"
     CDS             3300..4361
                     /gene="rffG"
                     /locus_tag="ACFOF2_001048"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC7107384.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            4574..5449
                     /gene="rfbA"
                     /locus_tag="ACFOF2_001049"
     CDS             4574..5449
                     /gene="rfbA"
                     /locus_tag="ACFOF2_001049"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC7107385.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            5504..6043
                     /gene="rfbC"
                     /locus_tag="ACFOF2_001050"
     CDS             5504..6043
                     /gene="rfbC"
                     /locus_tag="ACFOF2_001050"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC7107386.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            6205..8253
                     /locus_tag="ACFOF2_001051"
     CDS             6205..8253
                     /locus_tag="ACFOF2_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC7107387.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            8390..9649
                     /locus_tag="ACFOF2_001052"
     CDS             8390..9649
                     /locus_tag="ACFOF2_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC7107388.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            10016..11422
                     /locus_tag="ACFOF2_001053"
     CDS             10016..11422
                     /locus_tag="ACFOF2_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC7107389.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            11640..12854
                     /gene="gltS"
                     /locus_tag="ACFOF2_001054"
     CDS             11640..12854
                     /gene="gltS"
                     /locus_tag="ACFOF2_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC7107390.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            12953..13969
                     /locus_tag="ACFOF2_001055"
     CDS             12953..13969
                     /locus_tag="ACFOF2_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC7107391.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            13980..14177
                     /locus_tag="ACFOF2_001056"
     CDS             13980..14177
                     /locus_tag="ACFOF2_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107392.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            14195..14344
                     /locus_tag="ACFOF2_001057"
     CDS             14195..14344
                     /locus_tag="ACFOF2_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107393.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
     gene            14527..14679
                     /locus_tag="ACFOF2_001058"
     CDS             14527..14679
                     /locus_tag="ACFOF2_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107394.1"
                     /translation="MSIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEAC
                     TVKLQE"
     gene            14701..14994
                     /locus_tag="ACFOF2_001059"
     CDS             14701..14994
                     /locus_tag="ACFOF2_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107395.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
     gene            complement(15361..16761)
                     /locus_tag="ACFOF2_001060"
     CDS             complement(15361..16761)
                     /locus_tag="ACFOF2_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC7107396.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
     gene            complement(17069..18541)
                     /gene="pyk"
                     /locus_tag="ACFOF2_001061"
     CDS             complement(17069..18541)
                     /gene="pyk"
                     /locus_tag="ACFOF2_001061"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC7107397.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT         4733 a         4736 c         4616 g         4765 t
ORIGIN      
        1 ttaatatcta atatttaaaa tacaatgaat aaaaaacgag tcctattaat tgctagttat
       61 gattcttttt tgaatactgg atatgctgtt gcaaaagaaa ttaactattc aacaatcgat
      121 atttatatcc accaaacaaa aaaaaatatt ctttcggaat cacaattaag tttaaatggg
      181 ctttctttaa aagaaatcaa tttttttcat attgatgatt atttcgacaa attaatgtac
      241 cagaattatg atattgttat tctatcatta ggtaatggtt tactgacgcg tttttttcgg
      301 aatagtagca aacttaattt aacattacct atagtaatta ctctatttcc aggggtaatt
      361 tttggtgatt cagaaagtat tataactagg ttaggtgcta atattttatt gttcaataat
      421 tattatgatt accaaatagg caataagttc aaagaacgtt acaacttatc gtgtacaaat
      481 attttatatg gttatcctat cctacgaaaa aaacaccatt gctctgatgg caataaagtt
      541 tatttcattg atcaagtaaa aattccagat ggaaaaaaag aaagattatt aattctaaaa
      601 aaattaatag cactggctaa tcgctatcca cataaagatt tagtactatt attacgtgtc
      661 catcaaaatg atatcacagt acatgaagat aaatactctt atgctgaatt agcaaaaaat
      721 atgaaattgc ctgataattt taaaatagag agaaaacgga cacaagatgc cttacaagaa
      781 atgggatatt gtttatcatt ctcttcaaca atgttatttg aagctgaaag ctgcggaatt
      841 cctattggtg tcattagtga tttaggggtt gacaagaaat atgccaatac acatttcaaa
      901 gatagtggtg ttttggttaa tttagatacg gtagacttgg actctccaaa taccattaat
      961 ccaacttggg ctgaactata tactaatcca gatagcctct cttcaaaaga gtttaaccaa
     1021 ttattagagg ttacttctac tcaaccacct agttttgata attattttat tgcagttaat
     1081 gacgttcagc ctgtatctat ggttctgtta cgtaaattta aaaagcttat atatcatcct
     1141 aagcaattct ttgttgattc aaaactatat aaattaatta aatttactaa ataattttta
     1201 ctatgggttg ccttatttat ttcaaaggag tgacataaaa aatgcaaaaa attcgtaaag
     1261 ctgtattccc tgtagctggc atgggaaccc gattcttacc tgccacgaaa gccagcgcta
     1321 aagaaatgct gccaatcgtg gacaaaccgt taattcaata tgctgcggaa gaagccgttg
     1381 ccgcgggttg tactgaattg atttttatca ccggccgtaa taagcgtaat attgaagacc
     1441 acttcgataa agcctatgag ttggaaactg aactggaaat gcgtgaaaag cataaattgc
     1501 ttggctatgt gcaaaaaatt ctgcctgatc atgtcagctg tattttcatt cgtcaggcag
     1561 aagctttggg cttgggtcat gcggtattat gcgcccgtcc tgctgtcggt aatgagcctt
     1621 ttgccgtgat tttggccgat gacttgattg atgcaccaca aggcgcactc aagcagatgg
     1681 tagatattta caatcaaagc ggcaacagca ttttaggggt agaaacagtc gatccttcgc
     1741 aaaccggttc atacggcatt gtcgaagtgg aacagctgaa aagctatcag cgtgtaacca
     1801 atattgtgga aaaaccaaag cctgaagatg cgccgtcaaa tttagcggta gtcggccgtt
     1861 atattttgac accgcgtatt tttgatttgc tgactaatct acctcgtggt gcaggtggtg
     1921 aaatccagct aactgatggt attgcccgcc tacttgacca tgaatttgtc ttggcgcatg
     1981 cgtttgaagg taagcgttat gattgtggca gtaagttggg ctatctggaa gccacggtag
     2041 cttatggctt gaagcatccc gaattaaaag atgatttcaa agaattattg aagcaatttg
     2101 tataatttta aggccgtctg aaaaagaaaa ggcatcatgt tttttacact ttcaggcggc
     2161 cttttaaaca taaagaagga aaacgtgatg aacattctcg ttaccggcgg caccggtttt
     2221 atcggctcgc acaccgttgt ttctttgctg aaaagcggtc atcaagtcgt gattttggat
     2281 aacctatgca attccagcat caatatcctg ccacgcttga aaacgattac cggccaagag
     2341 attccgtttt atcaaggcga tatccgcgat cgtgagattt tgcgccgtat ttttgcggaa
     2401 aaccgcattg attcggtgat tcattttgcc ggcttgaaag cggtgggtga aagtgtggcc
     2461 gagccgataa aatattatga taataatgtt tccggcagct tggtgttggc ggaagaaatg
     2521 gcgcgtgcgg gcgtgtttag cattgtgttc agttcttcgg cgacggttta tggcgatccg
     2581 ggcaaagtgc cttataccga ggatatgcca ccgggcgaca ccaccagccc ttacggcgca
     2641 tcgaaatcga tggttgagcg cattctcact gacattcaaa aagccgatcc gcgctggagc
     2701 atgattttgt tgcgttattt caatccgatt ggcgcgcatg aaagcggttt gattggcgag
     2761 cagccaaacg gtatcccaaa taatttgttg ccttatatct gccaagtggc ggcaggcaaa
     2821 ctgccgcaat tagcggtatt cggtgatgac taccctaccc ctgatggcac gggtatgcgc
     2881 gactatattc atgtgatgga tttggcagaa ggccatgtcg cggctatgca ggcgaaaagc
     2941 aatgtagcag gcacgcattt gttgaactta ggttccggcc gcgcttcttc ggtattggaa
     3001 atcatccgcg catttgaagc ggcttcgggt ttaaccattc catttgaaat caaaccgcgc
     3061 cgcgccggtg atttggcgtg cttctatgcc gacccttctt atacgaaggc gcaaatcggc
     3121 tggcaaacgc agcgtgattt aacccaaatg atggaagatt catggcgctg ggtaagtaat
     3181 catccgaatg gttatgatga ttaagtcatg aaattgagag gccgtctgaa gtttcagacg
     3241 gcctttgttt gcgatttata gtaaaattta ccttatcatt ttggcgatta atattcatca
     3301 tgaaaaaaat ccttgtcacc ggcggtgccg gttttatcgg ttcggcggtt gtacgccata
     3361 tcatcaacga tacgcaagat gcggtggtga atgtggataa gctcacgtat gcgggcaatt
     3421 tggaatcgct ggaatctttg gccaatcatc cgcgctatgc gtttgagcaa gtcgatattt
     3481 gcgaccgcgc ggcactggat cgtgtgtttg ccgaacacca gccggatgcg gtgatgcact
     3541 tggcagcaga aagccatgtt gaccgcagta ttgatggccc agcggcattt attgaaacca
     3601 atattgttgg tacttacacg cttttggaag cggcgcgcgc gtattggcat gatatgccgt
     3661 ctgaaaaaaa agcggctttc cgtttccatc atatttcgac tgatgaagtg tatggcgatt
     3721 tgcacggcac ggatgatttg tttaccgaaa ccacgcctta cgccccttcc agtccttatt
     3781 cggcttccaa agcctccagc gaccatttgg tgcgcgcatg gctgcgtact tacggcctgc
     3841 cgaccattgt gaccaattgt tctaataact acggccctta tcattttcct gaaaaactga
     3901 ttccactgat gattctgaat gcgcttgatg gcaaaccgct gccggtgtat ggcgacggta
     3961 tgcagattcg tgattggctg tttgttgaag atcatgcgcg tgcgctgtat caagtggtca
     4021 cagaaggcga aatcggcgaa acttacaaca ttggtggcca caatgaaaaa gccaatattg
     4081 aagtggtgcg caccatttgc gccctgctgg aagaattggt gccgaacaaa ccgcaaggcg
     4141 tggcgcgtta tgaagatttg attacctatg tcaaagaccg ccccggccat gatgtgcgct
     4201 atgcgattga tgccgcaaaa atcggccgtg agttgggttg gcagccgcaa gaaacttttg
     4261 aaagcggcat ccgcaaaaca gtggaatggt atttaaacaa tcaaacttgg tggtcgcgcg
     4321 tgttagacgg cagctacaat cgtgaacgct taggcagtta aatagagacc tttgcaaact
     4381 ccccatcttt ggcacatttc ttcgttatgc gctgctcgaa agcttgccta tctttgtgat
     4441 atgtctgcgc tttctgcgct gctataactt cgaactgtac tcaaatctgt ggctttgcaa
     4501 aggcctcaaa taataaaaat acgttcagac ggcatgaagg ccgcctgaaa aactacataa
     4561 ggaaagtaaa ctgatgaaag gcattattct cgcaggcggc agcggtatcc gcctttaccc
     4621 gattacccgc ggcgtatcaa agcaattact accggtgtac aacaaaccga tgatttatta
     4681 ccccttgtcg gtgttgatgt tggccggaat ccgcgatgtg ctggtgatta ccacccccga
     4741 agacaacgat gctttccgcc gcttactggg tgatggcagc gatttcggta ttcaaatttc
     4801 ctacgccatc caacccagcc ccgatggttt ggcgcaagcc tttttaattg gtgaagaatt
     4861 tatcggcgat gattcggtgt gtttggtgtt gggcgacaat attttctatg gccagcattt
     4921 cagccaatcc ctgaaagaag ccgccgcttc cgtggaaacc aaaggtgcta ccgtattcgg
     4981 ctatcaagtg aaagatcccg agcgtttcgg tgtgattgag tttgatgaga atttcaaagc
     5041 cctgtcaatc gaagaaaaac cgaaacaacc taaatccgat tgggcggtaa ccggcctcta
     5101 tttctacgat aatcgcgtag tcgagtttgc caaacaagtg aagccgtctg aacgcggcga
     5161 attggaaatt accaccttga atcaaatgta tttggacgat ggttcgctca atgtccaatt
     5221 gctcggtcgc ggtttcgcat ggcttgatac cggcacacac gccagcctgt tggaagcagc
     5281 ctcgtttgtc gaaaccattg aaaacattca aaacctacaa gtcgcctgtt tggaagaaat
     5341 cgcatggcgt aatggctggt tgagcagcga agaaatcaga aacttggcaa agccaatgtg
     5401 taaaaatgaa tatggccgct atttattgcg cttggttggc aaagattaag aggttgcttt
     5461 taccagtttt caggccgtct gaaacgcttt ttaggaaaac atgatgaata tcatcgatac
     5521 ccgcattccc gatgtcaaat tgctcgaacc caaagtcttc ggtgacgagc gtggtttttt
     5581 tatggaaacc ttccgtgatg aatggttccg ccaaaatgta gccgagcgca ctttcgtgca
     5641 ggaaaaccac agcaaatcca cccaaggcgt attacgcggt ttacattatc aaaccgaaaa
     5701 tacacaaggc aaactggtgc gcgtggtatc aggcgaagtc tttgatgttg ccgtggatat
     5761 gcgtcgtgat agtcctactt tcggccactg ggtcggtgag gttttatcgg cagacaacaa
     5821 acgccaatta tgggtgccgg aagggtttgc ccatggattt tatgtgttga gcgatgaagc
     5881 ggaattcgtc tacaaatgca ccgactatta caacccgaat gcggaacatt ccctaatttg
     5941 gaatgatgcc acagtgggaa tcgactggcc tttattggga gaaccaagcc tatcggccaa
     6001 ggatttggca ggcaagatgc ttgctgaagc ggccacattt taattttcac ttttaggtca
     6061 tcgtgggtaa tttgtagcaa gatgacctaa tttttaccag ctagcctact aactaaattt
     6121 ctactatatt taaatagtca gaaaatttac acaaaatctg ttaataagat attatattgt
     6181 cagtaatatt ttttcctatt tattgtgatt cctgcctaca ttcctcttcg ctctttcggt
     6241 atacgtaaaa ttccgaatat tacgcaattt ctaccggaat attcacttaa caaaaccgta
     6301 ttcaacgccc aaaccatcat tggctggggc ttacgcccga ccacccacca agcacgtgct
     6361 tttgcacaat cacatggctt gccgtttgta gccttggaag acggtttttt acgctcactg
     6421 ggcttaggcg tggccggtta tccgcccttc gcactggttt acgacgatat cggcatctat
     6481 tacgacacaa cccgcccatc gcgtttggag cagttgattt tagccgcaaa catgccgtct
     6541 gaaatgctta accaagcgca gcaggccatt gatttgattt tgacgcaccg tttgtctaaa
     6601 tataaccatg ctctggattt ttcagacggc ctcgtacaag acagcaaaaa cattgttttg
     6661 gtaattgatc aaactttcgg cgatatggcg attcaatatg gcggcgcaga tgaagccaca
     6721 ttccgccaaa tgtttcagac ggccttagcc gaaaatcctg atgcggaaat ctgggtcaaa
     6781 acccatcccg atgtattgag cggcaagaaa cagggttacc ttactgattt ggcacagcag
     6841 ccgcgtgtgc gtttattggc tgaagacatt aatccgattt cgctgctgca agcggtggac
     6901 aaagtgtatt gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg
     6961 gtaacgtttg gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa
     7021 atcaaccgcc ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc
     7081 gcagcctatc tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc
     7141 tttgatgtca tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag
     7201 ttatattgcg tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta
     7261 ccctcttgcc gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc
     7321 gacgatgcac gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa
     7381 caacaccaca tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc
     7441 tccaacttag tgccgccgct gtcgctcgtt accgacgata tgggtattta cttcaatgcc
     7501 gaagccccat cccgccttga acacatcctg caaaaccaaa acttcgacga tcaagacttt
     7561 cagacggcct tgaagttgca aaaaatgctg accgaaaacc atatcagcaa atacaacgtc
     7621 ggcaactcag actttaccgc cccatcaacc gacaaaaccg tgatcctcgt tcccggtcag
     7681 gttgaggatg atgcgtctat ccgctatggt tcgccccaaa tctatcacaa tctggatttg
     7741 ctccgtaccg tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat
     7801 gtagtcagcg gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac
     7861 caaaccgccg aacaagccga catcctgacc tgtctccaat acgcagacga aatacatacc
     7921 atgacttcgc tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc
     7981 ctgccttttt acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc
     8041 cgcagacttg agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc
     8101 caccccgaaa cccatcaggc cataaatgcg gaaaccgcag cccaaatcct gatacgacaa
     8161 aaaaatatgc aaaaaaacaa caacggatta catcgcgggt gctttgccaa aaaattaggt
     8221 aaaatcaaac aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat
     8281 aaacttgcct ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt
     8341 gaaagttcaa cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac
     8401 cgtcctcaaa aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca
     8461 aggccctgtc ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac
     8521 cgtacataaa ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca
     8581 taccgttgtt tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac
     8641 tcaacatcac atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc
     8701 aaaacgcatt gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg
     8761 cccctactac atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg
     8821 tgccgacttt tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac
     8881 gccggtacac ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt
     8941 gttccgcaat ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca
     9001 ttacctcaaa ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat
     9061 ccaaatcgcc aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca
     9121 ggtattcaac gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct
     9181 gctccatgtt ttgagttcat ttgccgagca cgcgcctgcc gataccaaca tcatcatcaa
     9241 gcatcatccg atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa
     9301 agaacacccc gaactcaaag gccgcgtgat ttatgtccat gatgtccccc tgcccgtttt
     9361 cctgcgccac ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggactgat
     9421 tcacaatatg ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga
     9481 ccaaaatacc ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca
     9541 tgcctaccga atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt
     9601 gtttttcccc aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg
     9661 aagttcaggc cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg
     9721 ccatgtaaat aaaacacaaa aatctgcatt tatcattaac aataaattac aaaaacagta
     9781 taatgaccga gctgccatga gcgcataccg actcaacctg agccctttat aacacacaaa
     9841 atatggatgt atccccaggc aaaacaatat aacaagccaa acatcctaaa gataagccgg
     9901 caaggaaata cactctgcaa aacgatgccg agcaatattt ttacaaagcc ctcaaccggt
     9961 atcgccgccc atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac
    10021 cgccgctcct gatatcctat accgtcaagc cgccgccctt ttggaacaat ccaataccgc
    10081 ccaagccctg cccctgctgc aacaggccgc agagcaggga tatacggaag ctgcttttgt
    10141 attgggcgac catctgctgc aaaacggtca tccggagcag gcacttccat ggttggaagc
    10201 cgccgcggcc caacgccatc ccaaggcact ctttaccctg ctgcaacaac gcgaacacaa
    10261 cggcaccccg accggacagc ttcttaacga atacgcctgg ctgagtgagc aaggtcacct
    10321 cgaagcccaa ttaattctca tgcgttacca cacgcagcgc aacgatccac aatcgctcta
    10381 ctgggcggag cttgccgctg cccaatatgc cgcacctgcg tattaccatt tggcacgcca
    10441 tcatcaacgc caaggcgacg ttgcaacagc tatcgaacaa tacgaaaaag cggcagcgct
    10501 cggcgtgact gcagcctgct ggcaactcgg tcaaatctac ttctacggca caggtgtcag
    10561 ccccaaccac gcacaagccg aacactatct cgaacaagcc gcgcaagccg gccacatcgc
    10621 cgcacaaacc ctgctggccg accttcttgc tgcccaacgc aaacccgaag ccttagaatg
    10681 gtatcgtcgt gccgccgaca aagagcaagc ggaagcacag gccaagctgg ctcaatacgc
    10741 cctgaccggc gaactttccg aacgcgatcc gtttcaagcg gcacgatatg ccaaagccgc
    10801 cgctgagaaa gaccatcccg aagccctgaa aatcatgggc gacctctacc gctacggcct
    10861 cggcatcaaa gctgacaacc atatcgcgca cgattactac caccgtgctg ccgcgctggg
    10921 ttctgccgcc gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata
    10981 cgaacaaatc aaaactgccg ccctgcaaca gcaacaaacc gaaaccatct accgtttggc
    11041 ggaaacacaa gcccatgcca tcggtcgtcc cgccgactat aatgccgcac gcaaaaatta
    11101 catggaagct gccgggttcc accataaaaa cgcggcggct gccttaggcc gcatctacca
    11161 ttacggcctc ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccattgctgc
    11221 cgaacaaaac cacccttccg cccaatacca cctcgcctgt ttttactatc acgggcaagg
    11281 tgtcggctgt catgttccga ccgcctgcta ctggctgcag gccgccatcg gcaacggcca
    11341 cacttcggcc gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc
    11401 catcggacaa aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta
    11461 ttcccctaat atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga
    11521 aaacaccttg tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac
    11581 acactacatc cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca
    11641 tggaatggga attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg
    11701 gtaaatttct ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtcg
    11761 ctggcggttt ggttgccgcg attatcctgt ttgcgctgca cgaggcgtac ggcgtgagct
    11821 tcaaatttga gaaaccgctg caaaatgcgt ttatgctgat ttttttcacg tccatcggct
    11881 tgagcgcgga tttttcccgt ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga
    11941 ttgtgggcgg atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg
    12001 gtttggaccc gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta
    12061 cgtcaggtgc gtggggacct aattttgaaa cgcaatatgg cttggtcggc gcaaccggtt
    12121 tgggtattgc atcggctact ttcgggctgg tgttcggcgg cctgatcggc gggccggttg
    12181 cgcgccgcct gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg
    12241 acgacaacgc ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat
    12301 ctgccgttga aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg
    12361 gcttcgacaa agaatacctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg
    12421 tggtcatccg caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg
    12481 atgtgttcgg caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac
    12541 tgtgggagct gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg
    12601 tgatggtttt gtacgcgact tttgttacct atgtctttat ggggcgcgat tatgatgcgg
    12661 cagtattggc tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa
    12721 atatgcagtc cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta
    12781 tggtcggcgc gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt
    12841 tctttaaagg ctgattttcc gcctttccgc caaagcacct gcaaggttta ccgcctgcag
    12901 gtgcttttgc tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg
    12961 gaaactcatt gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc
    13021 caaggcattg gaatgcgcca accccgccgt gttgcaaggc atacgcggca atattcagga
    13081 aacgctcacg caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc
    13141 cgacaaaatt atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac
    13201 gcaggaaggc gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac
    13261 gcttgccgat gccaaggcaa acagccccct gctgtacggg gaaaccgctt tgtcggatat
    13321 tgtgcggcag aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt
    13381 ccgcttcctg cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc
    13441 ggcgcaaacg ctgtctgccg cgctgctgcc ttacggcgtg aagagcatcg tgatgataga
    13501 cggcaaggcg gtgaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga
    13561 agaaccgtcc aaacccacgc ccgaagacat tttggaacac aatgccgccg gcggcgatgc
    13621 gggcgtaccc caagccgcag aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg
    13681 cgagcgtgcc gataccgtta ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa
    13741 ccagcgtgcg gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa
    13801 agagttggtc ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc
    13861 cgccgcgcag gcagaccggc aggaatacgc cgaatacctc aagctgcaat gcgacacgcg
    13921 gatgacgcgc gaacggatac agtatcttcg cggctattcc atcgattagg ggcaaaccga
    13981 tgactaccgt ccccaaaagc aggattcccg tcaaaccgct gcccgaaaaa accacagacg
    14041 aagccaaagt cgaaaaatgg cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg
    14101 cagttgccgc cagattgggc gagaacggtt ttaccgaaga acagatggaa aatatcgcca
    14161 acctgttcgg cagataaagg gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt
    14221 ttcagacggc attttgacta atacggtacg caggcgcaaa acagccggct ttttatgtgt
    14281 ttcctatgct gatgttccaa cacacaggac gatacaaaaa gcgtcgccct atgtgccgtc
    14341 ctgattcgga aagggttacg ctcctcccaa atatagtgga ttaacaaaaa ccggtacggc
    14401 gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
    14461 ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    14521 actatattgt ccattcggca gggaaagcct gaagaagaat tttcccgtaa gatggccggc
    14581 tgcctgaccg acttggaaaa ctgtttggaa aaatacctcg aacagttcgg gccggtatcg
    14641 gaaagtatcg aagcctgtac cgtcaaactg caggagtaac cgtcgttctt caacaggctg
    14701 atgaaaacca acgacaagct caataggcag attgacgtgt tgcagaaaca gtcggcagcc
    14761 atccacaacg aagcctatat cgaaatgaac acgctgcttt accgccaccg cgaagtggtt
    14821 tccgtccaca accgcaaggc ggattatgcg gaaaagggca aagagcggat tgcgctgttc
    14881 ccgcgcggct tgaacggcat caccaagctg cctgccgccg tcctgttgcc cgagcgtccc
    14941 gattgttttg atatgaaggc agtttggcaa atgtttaata gaataccgag gtaggcgtat
    15001 cggctgccgg ataaaaatgc cgtctgaaac ggaagcgttc agacggcatt gttgcagtgg
    15061 aacaaatcaa aatgcacccc gtcattcctg tctgtacggt gatagcggca gtgttttcct
    15121 gcttcgtttt atagtggatt aacaaaaacc agtacggcgt tgtctcgcct tagctcaaag
    15181 agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt
    15241 ctgcggcttc gtcgccttgt tctgattttt gttaatccac tataaatcgc gcctaaaaca
    15301 atgccgtctg aaacggaaat ctgtttcaga cggcatttct acaatcaaac ggcgggccgt
    15361 ttatttgaat ttgtaggtgt attgcaggcc gatgatgtcg gcgtggtttt tgaaacgtgc
    15421 ggaagacgcg cctttgctgt ccacatcgtt gccgcttgcc ttcgccgtgc ggtagctggt
    15481 gtcgttgatg tggatgtggg tgtaggcggc atcgacgacg tggtttttgc cgatatggta
    15541 tttcatgccg gcggagaacc agatgcggtt gccgtcgggc aggctgttca tgcggtagtc
    15601 ggcgttgcgg acgggcgatt tgtcaaaagc gatgccggcg cgcagttgca gcggttcgct
    15661 gatttgataa gaaccgccga agccgacttt gtaggtgttg cgccagttgg gggtgatggt
    15721 ggtgcggtcg gatttgccgt tgacgatggt tttttctttt tcaaaaacca gttccgcctt
    15781 atcgaagcgg ctgtggcgcg tccaagttac gtcgccgaac aggtcggctt tatcggacac
    15841 tttgtacata ccgtgtacgg acaaagactc aggcgtaacg attttaacgc gggctttttc
    15901 attcgccgtg tagccgtttg ctgcaagcat cgtactccac attgctttcg ccgccgcgcc
    15961 gtctgccgcc cattcggcat cgcctttgag cgtgtgcgag actttggaac ggtagttcac
    16021 gcccacgcgc gcgcggtcgt tgatgtccca catccacgcc agttggtagc cgaagcccca
    16081 atcgctgcct ttgacatcgg cgtgtccgtc ggcctgaatt tttgcagctt cggctacacc
    16141 gttaggtttg ggcggttttg ccgtcaatat ctctgcttta ctcttaatcc cccagtcggc
    16201 atatttgcgc agttcggcgg aagtatgttg ggcgatgatg cctgcgccga aggaatggcg
    16261 gtcgttgagt ttccatgcgg cgacaggttc gacggcgatg ctggtcagac cgagtttgtt
    16321 gatgttgtgg cgcaacacgg aatctttttc gtattcggtg gcagagccga aggggacgta
    16381 cacgcccaag cccacggtca gattgtcgtt gactttgtat gcgccgtaga tgtggggcgc
    16441 gaccgtggtt ttggtgattt tgccgctttt cgaaccttgg acgggaagcc cggtaaagtc
    16501 ggtggcggaa tccgcctcat aatgaatgct gggcagcacg atgttggcgt tgacggaaat
    16561 ctggctgctg tcgagtttgg tcaggccggc agggttgtag aagatggtcg atgcgtcggc
    16621 ggcttctgcg gcggcggcat ttgccgtgct ttgcgcgttg accgactgtg tgccgaagtg
    16681 gtagccggag gcttgtgcgg atgcggcggc aaaggcagtg ccgagcagta ggacggtttt
    16741 tttcagtgcg gaaggggtca tttcggtttc cgtaaaaagg cggacggtgg ataaatatag
    16801 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
    16861 ggcaacgccg tactggttta aatttaatcc actataaaaa aggcagtcgg aaatgccttg
    16921 tttcgcttta gtataggtac tcaattttat ccgatgttgc cggatttgca caattttttc
    16981 agtttgcccg aaccgccgcg ccgccgcaaa aaatgccgtc tgaagcctcg ggcatcggct
    17041 tcagacggca ttttccactc agggcggatt atttgacgcg cagcacttcc agtgtgttgg
    17101 tcgaaccgga ttcgcgcatt tgcgaaccgc tggtgatgat gtactggtcg ccggaatgca
    17161 ggatgttgtg ttccaccagc atggtttcga cttcgttcaa cgccgtgtcg tggtcggtac
    17221 tggttgccaa aatcagcggg cgcacgccgc ggtacatcgc catacggcgt tgggcggaaa
    17281 cgctcggggt cagcgcgaaa atcggcaggg tgatgttgtg gcggctgatt tcaaaggcgg
    17341 tcgaaccgct ttcggtcagg gcgacaatcg ctttggcgtg aaccgcgcgc gccacgctga
    17401 ccgcgccgcc ggcaaccgcc aggttggtgc tgaccgcttc gggatactcc gcctgttcgg
    17461 caacgccgtt gagcgaatcc tgctcttttt ccgcagccgc gcagataatt gccatttggc
    17521 tgacggtttc aaacggatac gcgccgacgg cggtttcggc ggaacacatc accgcatcgg
    17581 taccgtccaa taccgcgttt gccacatcgc tgacttccgc gcgggtcggc acggggttgg
    17641 taatcatcga ttccatcatt tgcgtcgccg taatgctgaa gcggcgcaac tcgcgggcgc
    17701 ggcggatcat ccgtttttgc agggcgggga cggcggcgtg tccgacttcg accgccaagt
    17761 cgccgcgcgc aaccataatg ccgtcgccgg cgaggatgat ttcatcaagg ttttcaatcg
    17821 cttctacgcg ttcgattttg gaaaccaaac cggggcgcac ggcagtgctg cccttcattt
    17881 cttcttcgac tttggcgcgc gcgatgtgca aatcttcggc agatttcaca aagctgatgg
    17941 cgaggtagtc gcaaccgatg gcaatcgcgg ttttcaggtc gcggaagtct ttttcggtca
    18001 acgcgcctgc ggacagcccg ccgccgcgtt tgttgatgcc tttgttgctt ttcaggacat
    18061 ggctgttttc cacccttgtg ataatcctgc tgccttcgac ggattccacg gtcagggtca
    18121 gcaggccgtc gtccagccac aatacgtcgc ctgcggcaac gtcgtcgggc aggtcgcggt
    18181 agtccaaacc gaccgcctcg cgcgtgcctt cgccttcgag cgcggcatcc agtaccagcg
    18241 tttcgccttt gttcaattca atgccgccgc cggcgatttt gcccacgcgg attttcggcc
    18301 cttgcaggtc ggcaatgatg gcgatttcct gtccggcgcg ttttgccgcc tcgcgcacga
    18361 tgcgggcgtt ttcctgatgg aattcgggcg tgccgtggct gaagttgaag cggacgacgt
    18421 tcaggccgcc gacgcggatc atatcttcca acagttctac gttgttgctg cccggcccaa
    18481 gggtggcgac gattttagtg ttgtggctga tgcgggtcag atcgcggctt gtctggttca
    18541 tatgaaagtc cttttggtct caatcgggtg ttttgcggta ttttgttaca aaattacaga
    18601 aatttggaac cggtttgatg tccatttgat gaacgcggcg gaatattctg taaaaatatg
    18661 atttaaatta atagtttgat attttacctg caaaccgcct tttttggcgc aaaattacac
    18721 ggttttatga cttaggctaa atttattttg gggctgtcct agataactag ggaaattcaa
    18781 attaagttag aattatccct atgagaaaaa gtcgtctaag ccagtataaa caaaataaac
    18841 tcattgagct
//
LOCUS       DAXIHS010000035        18345 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXIHS010000035 DAXIHS010000000
VERSION     DAXIHS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.035"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            663..2111
                     /gene="gnd"
                     /locus_tag="ACFOF2_001062"
     CDS             663..2111
                     /gene="gnd"
                     /locus_tag="ACFOF2_001062"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC7107398.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPTWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2174..3445
                     /gene="waaA"
                     /locus_tag="ACFOF2_001063"
     CDS             2174..3445
                     /gene="waaA"
                     /locus_tag="ACFOF2_001063"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC7107399.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACWHALASGAAVQVESADAWREAVEKTLSSKGGGMQMQ
                     VRVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3486..3935
                     /locus_tag="ACFOF2_001064"
     CDS             3486..3935
                     /locus_tag="ACFOF2_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC7107400.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGLVSCEARRE
                     VENAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3968..4942
                     /locus_tag="ACFOF2_001065"
     CDS             3968..4942
                     /locus_tag="ACFOF2_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC7107401.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLSDVEERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5011..5086
                     /locus_tag="ACFOF2_001066"
     tRNA            5011..5086
                     /locus_tag="ACFOF2_001066"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5044..5046,aa:Lys,seq:ttt)
     gene            5306..6559
                     /gene="murA"
                     /locus_tag="ACFOF2_001067"
     CDS             5306..6559
                     /gene="murA"
                     /locus_tag="ACFOF2_001067"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC7107402.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6640..7818
                     /locus_tag="ACFOF2_001068"
     CDS             6640..7818
                     /locus_tag="ACFOF2_001068"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC7107403.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7885..8133)
                     /locus_tag="ACFOF2_001069"
     CDS             complement(7885..8133)
                     /locus_tag="ACFOF2_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC7107404.1"
                     /translation="MLTPEQVKAMIEDVAKCEHIKVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8223..9140)
                     /gene="ftsX"
                     /locus_tag="ACFOF2_001070"
     CDS             complement(8223..9140)
                     /gene="ftsX"
                     /locus_tag="ACFOF2_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC7107405.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPEEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9137..9787)
                     /gene="ftsE"
                     /locus_tag="ACFOF2_001071"
     CDS             complement(9137..9787)
                     /gene="ftsE"
                     /locus_tag="ACFOF2_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC7107406.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9784..9975)
                     /locus_tag="ACFOF2_001072"
     CDS             complement(9784..9975)
                     /locus_tag="ACFOF2_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107407.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(9983..10465)
                     /locus_tag="ACFOF2_001073"
     CDS             complement(9983..10465)
                     /locus_tag="ACFOF2_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC7107408.1"
                     /translation="MKYLNLAAITLGATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10589..10942
                     /gene="arsC"
                     /locus_tag="ACFOF2_001074"
     CDS             10589..10942
                     /gene="arsC"
                     /locus_tag="ACFOF2_001074"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC7107409.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10939..11595
                     /locus_tag="ACFOF2_001075"
     CDS             10939..11595
                     /locus_tag="ACFOF2_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC7107410.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFTSSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11711..13105
                     /gene="gltX"
                     /locus_tag="ACFOF2_001076"
     CDS             11711..13105
                     /gene="gltX"
                     /locus_tag="ACFOF2_001076"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC7107411.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13126..13521
                     /locus_tag="ACFOF2_001077"
     CDS             13126..13521
                     /locus_tag="ACFOF2_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107412.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            13525..14016
                     /locus_tag="ACFOF2_001078"
     CDS             13525..14016
                     /locus_tag="ACFOF2_001078"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC7107413.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14139..16733)
                     /gene="mutS"
                     /locus_tag="ACFOF2_001079"
     CDS             complement(14139..16733)
                     /gene="mutS"
                     /locus_tag="ACFOF2_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC7107414.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCTTHMGSRLLALWLHHPLRNRAHIRARQEAVTALDSQYEPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSTNGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17177..18181
                     /gene="gap"
                     /locus_tag="ACFOF2_001080"
     CDS             17177..18181
                     /gene="gap"
                     /locus_tag="ACFOF2_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC7107415.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTSDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
BASE COUNT         4202 a         4476 c         5277 g         4390 t
ORIGIN      
        1 actttcgtgg gaatgacggg atgtaagttc gtgggaatga cgcggtacag gtttccgtta
       61 cgtatcccca tccgaaagtt tacttataca caagcaatcc aaagttatct gactgaaata
      121 ttgtgagatt tacccgcaaa tctcattttc tccaccactt atacacaaac tcggatgcac
      181 actctgccaa tatgcagaga aaaagacatt cgtgccgtta acttactatt cccacacgtt
      241 ccaaacacat ttccctctca aacctccaca gtattcccgt tcttaaaggc gccagcagcc
      301 attcggtaaa gaaaagtagg atgccatctg cacaacaagc aaactgtcca caaaaactat
      361 tcttcatgaa aaacaatcca caggtatgca caaaagggaa tccgccttac cgacaccctt
      421 ttaaaacggc aatctcgatg atttttaaaa taaaaaaaag ttacccacag aaaatgtctc
      481 ctatcaatat aatcatcgtt ttattattta aattttatat tttatttata agccttcagg
      541 gaaacgcacg gtaaagcgta aaggcatctt ttcaaaaaat taaaaagtta aggattgtta
      601 aacaagtgta aatcaggtag agttgcggtc gataaattaa atctataacg gagcttcctg
      661 caatgaaagg cgatattggt gtaatcggtt tggcggttat ggggcaaaac ctgattttga
      721 atatgaacga ttgcggattt aaggttgtcg cctacaaccg aacaatcggt aaggtggacg
      781 aatttttaaa cggcgcggct aagggaacgg atattgtcgg cgcgtattcc ctgcaagact
      841 tggtcgataa gctggcaaaa ccgagaaaaa tcatgatgat ggttcgcgca ggttcggtgg
      901 ttgacgactt tatcgaacaa ctgcttccgt tgttggaaga aggcgacatc attatcgacg
      961 gcggcaatgc caattacccc gacacgacac ggcggacaca ttaccttgcc gaaaaaggga
     1021 ttttgtttgt cggcgcgggc gtatccggcg gcgaagaagg cgcgcggcac ggcccgtcta
     1081 tcatgccggg cggagatgag cgcgcttggg aagccgtcaa accgattttt caggcgattg
     1141 ccgccaaaac gccgcagggc gaaccgtgtt gcgactgggt cggcagggac ggcgcggggc
     1201 attttgtcaa aatggtgcat aacggcatcg aatacggcga tatgcagttg atttgcgaag
     1261 cgtaccagtt catgaaagac ggcttggggc tgtcttacga cgaaatgcac cgcgtgtttg
     1321 ccgagtggaa caaaaccgag ctggattctt atctgattga aatcacagcg gcaattttgg
     1381 gctataagga cgaaggcggc gaaccgctgg tcgagaaaat cctcgatacg gcagggcaaa
     1441 aaggcacggg caaatggacg ggcatcaatg ctttggattt gggtattccg ttgacgctga
     1501 tttccgaagc ggtattcgcc cgctgcgttt cgtcgttcaa agaacagcgc gtgcagaccg
     1561 gcaagctatt cgcccgaacc gttacgcccg tcgaaggcgg caaacaagaa tgggttgagg
     1621 cgttgagaca ggccctgctt gcatctaaaa tcatttccta cgcacaaggc ttcatgctga
     1681 tccgcgaagc gggcgaaagc tacggctggg atttggacta cggcaacact gcgctgctgt
     1741 ggcgcgaggg gtgcatcatc cgcagcgcat tcttaagcaa tatccgcgac gcgtatgaaa
     1801 acaatcccga tttggtgttc ttgggtgcgg atccgtattt caaaaatatt ttggaaaact
     1861 gcctgccgac gtggcgcaag gtggttgcca aggcggtcga atgcggcatc cctatgcctt
     1921 gcatggcttc ggcgattacg ttcttggacg gctacacgac cgaacgtctg cctgccaacc
     1981 tcttgcaggc acagcgcgac tacttcggcg cgcacactta cgaacgcacc gacaaaccgc
     2041 gcggcgagtt tttccatacc aactggacgg gcaagggcgg agataccgct tcgacaacct
     2101 acgatatttg attccgttgc cgaaatgccg tccgaaccgt tcggacggca tttgttttgc
     2161 aggaaataga aagatgttcc aatggcttta tgatgtattg tggctgcttg cgccgatatg
     2221 gatacggcgt tatttggaca aacgctccgg aagtgccccg gcatatcggg cgcatcggga
     2281 cgagcgtttc ggcaagccgt atccgaatcc cgttaccggc gcggtttgga tacacgccgt
     2341 ttcggtcgga gaaacgcgtg ccgcccagcc cttgatacgc gagttgcggc agcgttttcc
     2401 cgatgcgccg ctgctgatga cgcagatgac cccgacggga cgggaaaccg cgcaagttct
     2461 gtttcctgat gcacaatgcc gttatcttcc gtatgacaaa aaaacgtggg tacggcagtt
     2521 tttgcgcgaa caccgcccga tgttcggcat tttgatggaa accgaaatct ggcccaacct
     2581 gatgacggag tgccggaaat cgggcgttcc gctgtttttg gcgaatgcgc ggctgtcgga
     2641 aaaatcgcta aacggttatc tgaaagtccg ccgcctgatc cgtcctgccg ccgcttcgct
     2701 gacgggttgt ctggcgcaga cggaggcgga tgcggcgcgg ttggcgaaat tgggcgcggc
     2761 atccgtgcag gtgtgcggca ataccaaata cgacctgatg ccgtcggaac agatgaaaac
     2821 gctggcgggg cagtttgaaa aacgcatcgg agaccggcag gttgccgtgt gcggcagcac
     2881 gcgcgtttat cggggtgaag acgaggcgga aaaactgctg gcggcgtggc aacaatatcg
     2941 cggcgatgcg ctgctggtcg tcgtgccgcg ccatcccgag cattttcaga cggtatttga
     3001 aacggcaaaa cgcttcgggt ttaaggttca gcggcgcagc gacggtttgc cggtcgaacc
     3061 tgatacgcag gtgtggatag gcgacagtat gggcgagctg tatgcgtatt acttgtgcgc
     3121 cgatgtcgct tttgtcggcg gcagtctggt cgattcgggc tgtcagaaca ttatcgaacc
     3181 gctttcctgc ggcgttccga cgatattcgg cttttcaacc tacaattttt ccgaagcctg
     3241 ctggcacgcc ttggcatcgg gggcggcggt tcaagtcgaa tcggcggatg cgtggcgtga
     3301 agccgttgaa aaaaccttat cgtccaaggg gggcggaatg cagatgcagg tgcgcgtgga
     3361 cggctttatc gcacaacatc gcggagcggg cgcgagaatc gccgaggcgg tgcgggaagc
     3421 ggtatgcgga catcgggggc gatagtgata caatccgtat ccgagttttc cgtttggagc
     3481 atcgtatgag tatttatgcc gtcgcgcaca tcgttcatct gtattgcgct attgcctttg
     3541 tcggcggcgt gttttttgaa gtgctggttt tgtccgtcct gcatacggga ctggtgtcgt
     3601 gcgaggcgcg gcgcgaagtg gaaaatgcaa tgtcttaccg cgccgtcagg gtgatgccgt
     3661 ttgtggtcgg actgctgttc gccagcggca tcgtgatggc ggcaaaccgc tatctttcta
     3721 tattgggcga accgtttgcc acttccttcg gtacgatgct gacgctgaaa atcctgttgg
     3781 cgttcagcgt gttggcgcac ttcgccatcg ccgtcgtcaa aatggcgcgt tccacactga
     3841 ccgtcggctg gtcgaaatac atacacaccg tcgtctttac ccatatgctg ctgattgtct
     3901 ttttggcaaa agcgatgttt tatatcagct ggtaacacaa ttacatttca aatcaggaga
     3961 ttgaaccatg acagaatatc ccggcatcgg gtcgccgttg ttttacggcg ttttttttgc
     4021 ggcagtgctg gtcatgattg ccttggatat gttttcgctg aagaaaaacg gcagccataa
     4081 agtcggcatc aaagaagcct tggcatggag cggcttgtgg gttgccgtat cctgcctgtt
     4141 cgcgggctgg ctgtatttcg aactcgccgg caatcccggc tacggcgcgg cagtcgcaaa
     4201 agaaaaagta ttggaattct ttaccggcta cattttggaa aaatcgttgg cagtcgataa
     4261 tattttcgtg ttcctgatga tattcggcta cttcaaagtc gcaccgcagt ttcagcaccg
     4321 cgtgctgctg tacggcgtat tgggcgcatt ggtattgcgc accgtcatga ttttcgtcgg
     4381 cgcggcactg gttcggcagt tcgagtggat tctgtatctg ttcggcgcgt tcctgctcta
     4441 taccggcata cacatgatga agcccgaagg cgatgaggaa ggggatttgg caaacagcag
     4501 gctgctgaat gccgtcaaga aagtcgttcc ggtcggcacg gaatttcacg gcgagaaatt
     4561 ttttaccgtc gaaaacggca aaaaaatcgc cacgccgctg tttttggtgc tggtcatgat
     4621 tgaattgagc gatgtcgtgt ttgccgtgga cagcatcccc gccgtctttg ccgttaccac
     4681 cgatccgttt atcgtgctga cctccaatat tttcgccatt ttgggtttgc gggcgatgta
     4741 tttcctgctg tcggatgtgg aggaacgctt tatcttcctg aaatacggct tggcattcgt
     4801 gttgagtttt atcggtgtga aaatgctggt aatgcattgg gtgcatatcc cgatttccgt
     4861 ttcgctgtcg gtcgtgttcg gcgcgttggg tgcatcgata ctgacctcgt taatttatac
     4921 gaaaaaacag cctgataaat aaaatgccgt ctgaacgccg acaaaatggt tgacagcatc
     4981 aggcttgatg tatagaattt cgagcttatc gggtcgttag ctcagtcggt agagcagcgg
     5041 acttttaatc cgttggtcga gcgttcgaat cgctcacgac ccaccagata aaacaaaaaa
     5101 agctgagttt tcaaaactcg gctttttttg tcttccgaca atccttgttc ggaaagcgcg
     5161 gcatttcgtc aaattctttg gtgcggcttc cgtatttttc ccgaaaatcc cttataattc
     5221 cgcctctgca accttcatcg ggtgatgctt tatgcgttgt cccgagtatt gaaatattac
     5281 aagtacccac caggaaagaa caaacgtgga caaactgaaa atctccgcaa acggcccgct
     5341 caacggggaa ataacggtct cgggcgcgaa aaacgcggca ttgccgctga tgtgcgcggg
     5401 tttgctgaca tcgggtacgt tgcgcctgaa aaatgtgcct atgctggcag atgtgaaaac
     5461 cacacaaaag ctgcttcagg ggatgggcgc gcgcgtcctg accgacaata tcagtgaatt
     5521 tgaaatcaac ggtggtacgg taaacaacac ctgcgcccct tacgaattgg ttcggacgat
     5581 gcgggcctcg attttggtgc tggggccgac gctggcgcgt ttcggcgagg cgcaagtcag
     5641 cctgccgggc ggctgcgcca tcggttcgcg ccccgtcgat cagcatttga aaggcttgga
     5701 agcgatgggt gctgagattg ttatcgaaca cggttacgtc aaagccaaag gcaaactcaa
     5761 aggtacgcgc gtggcgatgg atgtcgttac cgtcggcggc acggaaaacc tgctgatggc
     5821 ggcgacgctg gcggaaggta cgacggtttt ggaaaactgc gccattgagc ctgaagtggt
     5881 cgatttggcg gaatgcctag tcaaaatggg cgcgaaaatc agcggcatcg gtacgtccac
     5941 aatgattgtg gaaggtgcgg acgagctgca cggctgcgaa cacagcgtcg tccccgaccg
     6001 gattgaggcg gggacgttcc tgtgcgcggt ggcgataacc ggcggcaggg tggttttgcg
     6061 gaatgccgcg ccgaaaacga tggaagtggt gttggacaaa ctggttgagg caggtgcggt
     6121 gattgaagcg ggcgacgatt ggatcgccat cgatatgcgg cagcgtccga aggcggtgga
     6181 catccgcacg gtcgtccacc ccggcttccc cacggatatg caggcgcagt ttatggcatt
     6241 gaatgccgtg gcggagggaa gctgccgcgt ggtggaaacg atttttgaaa accgctttat
     6301 gcacgtcccc gagttgaatc gaatgggggc gaacattaca accgagggca atacggcatt
     6361 tgtgcagggt gtggaacagc tttccggcgc ggtcgttaag gcgacggatt tgcgtgcgtc
     6421 cgccagcctc gttatcgccg gtttggcggc gcgaggcgaa accgtggtcg aacagattta
     6481 ccacttggat cgcggttatg aaaatattga aaaaaaactc ggcagcgtcg gcgcgaaaat
     6541 cgagcgcgta tccggctgat cgggttgcga tgccgtctga agccttcaga cggcatcaat
     6601 tgtttctgta tcgcaaacac tgattgaaaa ggactgatta tggcattttt aaaactgacc
     6661 gaacaaaacg tgcagggcaa aaccgtcctc atccgcgccg atatgaacgt gccgttcaaa
     6721 gacggcaaaa tcagcgacga cacccgtatc cgcgcctcgc tcgcgtccat ccaatactgc
     6781 atggacaacg gcgcatccgt tatcgtgatg atccacttgg gtcgcccgac tgaaggcgag
     6841 ttccaccccg aagacgacgt tgcgcccgtt gccgcgcatt tgggcggtct gttgggcaaa
     6901 gacgtgaaag tattgaacga ctggcgcgaa aacaaaccgg ctttgaacgc gggcgatgtc
     6961 gtgatgctgc aaaacgtgcg tatcaacaaa ggcgagaaga aaaacgattt ggaactgggt
     7021 aaagcctacg cttctttgtg cgacgtgttc gtcaatgacg cgttcggcac cgcgcaccgc
     7081 gcccaagctt cgaccgaagc cgtcgctcaa gccgcgcccg ttgcctgcgc cggcgtattg
     7141 atggcgggcg aactcgacgc tttgggcaaa gccctgaaac agcccgcgcg cccgatggtt
     7201 gccatcgttg ccggcagcaa agtgtccacc aagctgacca tcctcgaatc gctggcggat
     7261 aaagttgacc aactcatcgt cggcggcggt atcgccaaca ccttcctgtt ggcggaaggt
     7321 aaagccatcg gcaaatcttt ggcggaacac gatttggtgg aagaatccaa aaaaattatg
     7381 gcgaaaatgg cggccaaagg cggttctgtg ccgctgccga ccgatgtcgt cgttgccaaa
     7441 gcctttgccg ccgatgccga agcggtcgtg aaagacattg ccgacgttgc cgaagacgat
     7501 atgattttgg acatcggtcc gaaatccgcc gccgagcttg ccgaactgct caaagccgca
     7561 ggcacggtcg tttggaacgg ccctgtcggc gtgtttgagt ttgaccaatt cgcaggcggc
     7621 acgaaagctc ttgccgaagc cattgcccaa agcaaagcat tctcgattgc aggcggtggc
     7681 gacacgctgg cggcgattgc taaattcggc gttaccgacc aaatcggcta tatttctacc
     7741 ggcggaggcg cgtttcttga gttcttggaa ggtaaagagt tgcctgccgt agccgctttg
     7801 gaaaaacgcg gcgcgtaagc ggtttgatat aaaaaatgcc gtctgaaacg ttgtgctgtt
     7861 tctgacggca tttttgtgtg gcgattattg tgctttggct gcccattccg ccggagtggc
     7921 ggcaaccgaa atggaaagtg cgtgcagttc gttactttcc agttgggctt tgagtccgtc
     7981 tttaatcagg cggtggcgcg cgaggcgtgc cttgccttca aattctgatg aaacgatgac
     8041 ggcgaaaaaa tggtgtccgt cgccttctac tttgatatgt tcgcattttg ccacgtcttc
     8101 aatcatggct tttacttgtt ccggggtcag catggtaggt tccttgttgg aaaaatgcgc
     8161 cattatactg gcaaattgaa atgccgtctg aaacgggttc agacggcatt tttgacggtg
     8221 ttttattttt tggctttgaa gccgagcagg tgctgggtgg tggcaagcca cgcgccgaat
     8281 acgcccaacg cgatgacgaa gccgaacacc agcacgattt cttcagggta aaagaaacgc
     8341 cagccgatgt tcaagccgta gggtttgaaa atggcatcga ccaatgggcg cacggcagag
     8401 agcagccaac cgcaaagccc caagctgacg gcggcggaaa ggatgctctg ccacattgcc
     8461 tgataaagga aggggcggcg gataaacgac gcgggcgcgc ccaagagttt ggtgatttcg
     8521 atttcttctt ttcggctgag gatttgtagg cggatggtgt tgtgcgcgac aaggacgaac
     8581 gccatcccca gcgtcaggga aagaaaccat aaaattttgc ggatgaactc gttgatttgg
     8641 tacagcgttt gcacccattc ggtatccata gacgcggatt cgaccatagg cagtttggta
     8701 atgtctcggt agattgcctg catttgggcg ggcgtggttg ccgggtcggg ggtaacgata
     8761 aagacatccg gcagggggtt gccgtcaagc atggaaatca gattttggtc gagattggac
     8821 tgtaattctt ccagaccgtc ttctttgccg atgaagcgga tgttgtcgag ccgtttgtcg
     8881 cgcgccagca ggctgcgcac ggtatcgctg tcgctttgtg cggcggcggt ttccatatag
     8941 attgtgattt gcggcgactc gttgagtttg cccaacacgc tttgcccgct ttggatgccc
     9001 aaatacataa acagcggcag ggtcatggcg acggcgagca ttatgagggt aagcagtgtg
     9061 ccgaaaggtt ggcgcagaag ctgtttgagc gcggtgcgcg cggattcgac gtgcagcgag
     9121 aggtagtgga tgatgctcat gcgagtcgtc ctttcgagag gcgcaggatg cggtgtccgt
     9181 agtccgccat cagggtttcg tcatgtgcgg cgacgatgac ggttgttccc gcttcgtgga
     9241 aggttttgaa caattccata atatcgagcg cgtaggcgcg gtcgaggttg gcggagggtt
     9301 cgtcggcaat cagcaggccg ggctggtgaa cgacggcgcg ggcgatgcac aggcgttgtt
     9361 gttcaccgcc ggagagggtt acgggatcgt ccaattctcg tcctttcagg ccgacttttt
     9421 cgatggcgat gcgggcacgc tcttcggctt tgcgcggcgg atagccgata atccgaagcg
     9481 gcaggatgac gttttgcagg acgttgcggt cgtagaggat tttgtggtct tggaacacga
     9541 tgccgatgtg ttggcgcata aagccgattt ggttgtcgga caatgtgccg aggtcctgcc
     9601 cgttaaacag gattttgccc ctgcttggtt tggtaatgcc cgaaatcagc ttgaggacgg
     9661 tggatttgcc cgaaccggag tgtcccgcga taaatatcat ttcgcctttg ttgatttgga
     9721 agctgacgtt tttcagggct tcaaaaccgc cgggataggt tttggaaact tgttcgaaac
     9781 ggatcatagg aggtcctgta aggaatgggc ggcatatcgt ttcgggcggt tgcgtccgca
     9841 gggatatgcc gtctgaacaa taggtaagac agtgccgatt ataaagattc cgcctgtttt
     9901 tagatagtgg ataagtttgg cggataccga aggtcgggcg ggaaaaatgc cgtctgaagc
     9961 ggattcagac ggcatccggc gtttaacggc attttgaatg ggcgagtttg acggcttcgg
    10021 tcaggctttt ttcgttcact tccccggtaa tggtctgcct gtatccgcat ttcggtgctt
    10081 cgacgacggt aaagggcagt acgccgacag tgtttccgta ggttttcata aagtttcggc
    10141 tgttcgcccc agtgtaacgc caaatcgggt aagaaacagg ggtttgcttg aggaagttgc
    10201 cgatattgtc ggatgtgtcg agcgcgatgc cgaccatatc gacgctgcct tttttctgcg
    10261 ctttgtacca tttggacatg gcaggcatct cttttcggca cggaccgcac caggtcgccc
    10321 aaaggttgac gatgcggacg ggggctttga gcgattgcag gctttgcgcg gcaccgtctt
    10381 tccatccggc cagttcgtct gccgaggcgg tatgtgcggc aaatgtggcg ccaagggtga
    10441 ttgcggcaag attcaagtat ttcatcgttt tgtcctttgt atgtcgattc gggtattgta
    10501 aaccgtttgg tacggttgta ggcgggatat tgagtaaaac cctgtgtaag ccgataataa
    10561 ggggattgtc gagaagtaag gaaatgtcat gtctgaaatc aaaattttcc acaatccgcg
    10621 ttgcagcaaa tcgcgtgccg ccttgtccct attggaagaa cgcggcattg ctgccgaagt
    10681 ggtcaaatat ttggatacgc cgcccgactt gtccgaattg aaggatattt tcaacaaatt
    10741 gggcttggag tcggcgcgcg ggatgatgcg tgtgaaagat gatttgtaca aggaattggg
    10801 tttggacaac cccgatttgg acaataacgc actgctgcgt gccatcgccg atcatcccgc
    10861 cctgttggag cgtccgattg ttttggcaaa cggtaaggcg gctgtcggca ggccgttgga
    10921 aaatatcgaa gcggtattgt gaacaaacgg ctgttttgca gccgcaacgg cttgcgctat
    10981 tacctgttgg gcggtttctg tttgagtgtt tttccgcttt tgctggtgtt tacctcatcc
    11041 gtttgggcgg tttatcggac gggcgggcag gttttgccgc cgtatgtccg ggcggatgcc
    11101 gcgctggtgt tgggtgccgc cgcatgggac aaacgccctt cccctgtttt tcgggaacgc
    11161 atcaaccacg ccatcgcgct ttaccaaagc cgcagggtag gcaaaatcgt gtttacggga
    11221 gggcgaacca aaaaaggcta tatgaccgaa gccgaggtcg ggcggcggta cgcgctcaaa
    11281 cagggggttc cggcgcgcaa catcctgttt gaaaacactt cgcgcaacac ttatgaaaac
    11341 ctgaacaaca tccgtccggt tttgcgtgcc aacggtattg ccagcgtggt gattgtcagc
    11401 gatccctacc atttggcgcg ggcggcggaa atggcggagg atttgggtgt ccgcgtttat
    11461 atgtctgcca ccccgaccac gcggtttgat gcgggaaata agaaaaaaat ctttatgctg
    11521 caagagggat atgcgttatc cctctaccgt ttggaaaaat ggggcagccg ctttttggga
    11581 tggctgtctg attgaagcgg tagaaaatgc cgtctgaaac ttatccgcag gcagggttcg
    11641 gacggcattt ttaatgccca agcacggtaa aaacagtaaa ataagccgtt ttaaagattt
    11701 tggagccatc atgaccgtca aaacccgttt cgcccccagc ccaaccggct acctgcacat
    11761 cggcggcgta cgcaccgccc tgttttcttg ggcgtttgcc cgccatcata aaggcgaatt
    11821 cttattgcgc atcgaagaca ccgacttggc gcgctctact gccgaatccg tcaacatcat
    11881 cctcgacggc atgaaatggg tcggactcga ttacgacaac gccggcaacg tcgtgtacca
    11941 aacccgccgt ttcgaccgct ataaagaagt catcgccgaa cttttagaaa aaggcgatgc
    12001 ctactactgc tattgcagca aagaagagtt ggaagccatg cgcgagaaag ccgaaaaaga
    12061 aggcacggcg acttacgacc gccgctggcg gccggaagca ggcaaaaccc tgcccgaaat
    12121 ccctgccggc gtgcaacccg tcgtccgctt caaaacgcct ttggatggcg taaccaagtg
    12181 gacggacttg gtcaaaggcg aaatctccat ccccaacgaa gcactcgacg acctgattat
    12241 cgcccgcgcc gacggcacgc cgacctacaa cttctgcgtt gtcgtggacg actacgacat
    12301 gggcgttacc cacgttatcc ggggcgacga ccatgtgaac aacaccccga aacaaatcaa
    12361 catcttaaaa gccatcggcg ctaccctgcc tgaatacggc cacctgccga tgattcttaa
    12421 cgaacaaggc aaaaaaatct ccaaacgcag cggcgatacc gttgccatta ccgatttcgg
    12481 cgcgatgggc atcctgcccg aagccatgct caactatctg gcacgtttgg gctgggcgca
    12541 cggcgacgat gagttcttca cgatggaaca atttatcgaa tggtttgatt tgaaagacgt
    12601 ttctccatct ccaagccgca tggatttgaa gaaactctac tggatcaacg gcgaacacat
    12661 caaaatcacg cccaacggca aactcgccga actcgtcaaa ccgcgtctcg ctctgcgcga
    12721 tatccatgaa actgaaaaac ctgcattgga agacgtgttg gcactggtca aagaccgcgc
    12781 ccaagacctg aacacacttg ccgacgagtg cctctacttc tacgtcaaac aaacgcctac
    12841 tgaagcggac gtgcagaaac actgggacga tgaagccgcc gcccgtatgc tgcgctttgc
    12901 cgaacgcctc gaagggctgg aagactggaa tgccgaagcc atccacgacc tcttcaaacc
    12961 cttctgcgac gaagaaggca ttaaaatggg caaactcggc atgcccctgc gccttgccgt
    13021 ctgcggtacg gcgaaaaccc ccagcgtcga tgctgtgttg gcattaatcg gcaaagaaga
    13081 agtgttgaaa cgcatccgcg cttaagttat ccacaggcgt gggcgatgcc gtctgatgtc
    13141 ggaatgcggc ttcagacagc attcaaacgg aagttgaaca tgaaaaaaat attgtatttt
    13201 ctgatgtttg ttttttctac aagcgtatgg gccggggatg cggaagacaa tctgctcagc
    13261 atccaatccg gctatcgtgc cttactgcaa aaacaaaaca atctggacgg aaaaatcatc
    13321 gggatgcagt cggatttgga agatgcgcgc cggcgtttgc agacggctca agcagacatc
    13381 gcccgtttgg aagcggaaat tcctgcagca atggcgcaaa aagcccggca ggctgaagag
    13441 ctgaggcaaa tcggagtgcg tttggactat gcttggaatg cggtttacgg cgcaggggga
    13501 acgaaagcgt cggggaattg accgatgaat tccctctttg tggacaatac tgttttcatt
    13561 acacggctga aagccgggca tatcggcagg ttggttcagg cgttgtttga ggagtggcac
    13621 ggatttgaac cgtggtcttc tgtggataag attcatgcct attacggcag gtgtttgaag
    13681 gatgacgaac tgccgctggc atttgcggct gtggatgatt tcggaaccct gttgggttcg
    13741 gctgcggtca agcggcatga tatggaaagt tttccacagt atgaatattg gttgggtgat
    13801 gtctttgttt tacctgaata tcgcggaaaa ggcattggca ggaggctggt cgcccactgc
    13861 ataggcgcag cgcgttcgct ggggataaag ttcttgtatc tttatacgcc tgatgtgcaa
    13921 atattttatg aatcattcgg ctgggcggtt gtcgggcgac atttccataa cggtgaatgg
    13981 gttacggtta tgcgtttgga tatggataag gtttaagccg aatggacaaa tgccgtctga
    14041 gctgttcgga cggcattttt atgtttggaa acttcttgtc agataatgtt tatccacaga
    14101 tttttccata tggtttgatg ctgttccgac aacggaaatt aagatacgga tttgcacaaa
    14161 tctttcagac ggtacagttc tgacaatgct tcgcgcgggg tcaggctgtc gggatcgagt
    14221 ttttccaagg ctgctgccaa tataccttca aaatgttttt cctctgcttt atccacaaag
    14281 ttgcccacat tcggttcatc tcctttttca gacggcatgg tactgaaaat atccagttgg
    14341 ggacggttcg cggcggcttg gttttccagt ccgttcaaat gcttttgggc ggatttcaat
    14401 gcgcgtacag gcaggccggc gagtttggca acggcgatgc cgtagctttt tccggcggga
    14461 ccgggttgga tttggtgcag aaacacgatg tcctgtccct gttcgagcgc ggaaaggtgc
    14521 atattgagtg cggcggcgtg ggcttcgggc aggtaggtca gctcgaaata gtgggtggca
    14581 aacaggctga aggatttgtt tttttgcagc aggtgttcgg caacggcgtg cgcgagggcg
    14641 aggccgtcga aagtggaagt gccacgtccg acttcgtcca ttaaaacaag gctttgttcg
    14701 gtggcgtgat gcaggatgta ggcagtttcg ctcatttcga ccatgaaggt ggagcggttg
    14761 gaggcgaggt cgtccgatgc gccgatgcgg gtgaagattt gatcgatggg cccgattgtg
    14821 gcggcatcgg caggcacgaa acagccggtg tgtgccaata aaacaatcag cgcgacttgg
    14881 cgcatgtagg tggatttgcc gcccatattg gggccggtga gcagcatgag gcggtgtttg
    14941 tggtcaaggt cggtgtggtt ggcggtgaag tggcgtacct gctgttcgac aacgggatgg
    15001 cggccgtttt cgatgtggat aaccggatag tcggcaaact cggggcggac gaagttccgc
    15061 tcttttgcca aggctgaaaa tgtggacaat acgtccagcg cggcggcggc tttggcggct
    15121 ttttgaagct gcggcaatgc cgtctgaagg tttttcaata cgccgtcaaa gagttgtttt
    15181 tctaaggcga gggcttgctc ttgagcagtc agtactttgt cttcaaaggc tttcagttcc
    15241 ggcgtgatga agcgttcggc gtttttgagg gtttgccggc gttggtagtc ggcaggtgct
    15301 tgttcggctt gggttttgga caattcaatg taaaagccgt gaacgcggtt gaactcgact
    15361 ttgagtgtgg acaaaccggt acgttcgcgt tccttggctt ccaaatccag caaaaattcg
    15421 tcgccatggt tttgaatgcg gcgcaattcg tccagttcgg gatgaaaacc gtggttgatg
    15481 acattgccgt cttttagcca gacggaaggt tcgggcaaaa tggcttggcg gagctgttcg
    15541 gctgtggata ggttttccgg gaaaacggct ttgagggttt ccaacaggct gctgccgttt
    15601 gtggacaagt cgatttctga cagggcaaac aggctgtcgc gcagggcggc gaggtcgcgc
    15661 gggcgggcgt tgccgacggc gatgcgggca gcaatgcgtt caatgtccgc aatgtttttc
    15721 agacggcatt ggaggggttc gtattggctg tccagcgcgg ttacagcttc ttggcgggcg
    15781 cggatgtggg cgcggttgcg caaagggtgg tgcagccaga gagccaagag gcggctgccc
    15841 atgtgggtgg tgcaaaggtc gagcgtggac atcagggtcg gcgatttttt gccggagagg
    15901 gtttgcgtga tttcgagatt gcggcgcgtg gcggcatcca taccgatata ttggctgtcg
    15961 gtttcgagcg acaggccgtc caagtgttgc ggcatcaggt tttgcgtcag gcggatatag
    16021 ttcaacagtg cacctgccgc gccaatcgca acggcgtgtt ctttgccatc caaaccgaag
    16081 ccgcgcaaat cctggcagcc gaaatattcc gtcagcagtt tttcccccgc gtcggcggca
    16141 aactgccacg cgttcaggcg cgtaacaccc gatgccgtct gaagttgcgg tgcgttttta
    16201 ctgtcaggca acagaatttc cgccgcctgc aggcgcgcca gttcgtcgtc caatttatcc
    16261 acagtcgtca gcttggtttt gaattcgccg ctttgcagcg atgcccacgc caaaccgatg
    16321 tattttttgt cgggggacac ggcaacgatg cggttggttt ccttgtcttc cagcaatgcg
    16381 gaatcggtta gcgtgccagg cgttacgatg cgcacgactt tgcgttccac cggccctttg
    16441 cctgcgccga cttcgccgac ctgttcgcaa atcgccacgc ttttgcccaa cttgaccagg
    16501 cgcgccagat attgttcggc ggcgtgaaac ggcacgcctg ccattttaat cggtacgccg
    16561 tccatctgcc cgcgcgtggt cagggtaata tccaaaagtt ttgccgcttc taccgcatcg
    16621 tcgaaaaaca tctcgtaaaa atcgcccata cggtaaaaca ccagtttgtc ggtatgttgc
    16681 gctttgatgc cgaggtattg ctgcatcatt ggggaaacgg cggatttgga catggggctg
    16741 ccttatcatt aggaaaacgg tattgtagca aaaggttttg tcttcgacgg aatgagactg
    16801 accgtaagcc tgtcaataaa gttgtggaga caagtaggaa aacagcaaag ttatccacag
    16861 gaatattatt aagtcaaaaa acatccacgt ttatctgtcc tatacggcaa gccttaaact
    16921 caaacttatt tgacgaaaag gtaatgaaaa tattggatat ttgcaactta tacacaagca
    16981 gatccgcaat catcaaaatc atcattaaaa ttttaaattc aaatattgat ggtaaaagtt
    17041 ttggaaatct gtgcaaacag taaaattcat ctatatcaaa taagcatcaa gtagttagat
    17101 gtaatagtgg aatgtaactt tatttcagaa tacaatacag atattgaaat ttcgtttcac
    17161 agcttggagt atatacatga gcattaaagt agcgattaac ggtttcggcc gcatcggccg
    17221 cctcgcattg cgtcaaattg aaaaagccca cggcatcgaa gtcgtcgctg tcaacgacct
    17281 gacccctgcc gaaatgctgc tgcacctttt caaatacgac agcacgcaag gccgtttcca
    17341 aggtactgcc gaattaaaag acgatgccat cgtggtaaac ggcaaagaaa tcaaagtttt
    17401 cgccaatccg aatcccgaag aattgccttg gggcgagttg ggtgtggatg tcgtcctcga
    17461 atgtaccggc ttctttacca ataaaaccaa agccgaagca cacatcaggg ccggcgcgcg
    17521 caaagtcgtg atttccgcac ccagcggcaa tgatgtgaaa accgtcgtat atggcgtgaa
    17581 ccaagacatt ttggacggca gcgaaaccgt tatctccgct gcttcctgca ccaccaactg
    17641 ccttgccccg atggcggcag tcctgcaaaa ggaattcggc gtagtcgaag gcctgatgac
    17701 caccatccac gcctacacca gcgaccaaaa cacccttgac gcgccgcacc gcaaaggcga
    17761 tttgcgccgc gcccgcgccg ccgcgctcaa catcgtgccc aacagcaccg gcgccgccaa
    17821 ggccatcggt ctggttatcc ctgaattgaa cggcaaactc gacggctccg cccaacgcgt
    17881 ccctgttgcc accggctcgc tgaccgaatt ggtttccgtc ctcgaacgcc ctgtaaccaa
    17941 agaagaaatc aacgccgcga tgaaagcagc cgccagcgaa tcttacggct acaacgaaga
    18001 tcaaatcgtt tcttccgacg ttgtcggcat cgaatacggc tcgctcttcg atgcgaccca
    18061 aacccgcgtg atgaccgtgg gcggcaaaca attggtgaaa accgttgcct ggtacgacaa
    18121 cgaaatgtcc tacacctgcc aactcgtccg cactttggaa tactttgcag gcaaaatcta
    18181 aggcttgaac aaacctgtgg ataagtaaat gccgtctgaa cacattgcgt tcagacggca
    18241 ttgtttatct gtatagtgga ttaaatttaa accagtacgg cgttacctcg ccttgccgta
    18301 ctatttgtac tgtctgcggc ttcgttgcct tgtcctgatt taaat
//
LOCUS       DAXIHS010000036        18182 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXIHS010000036 DAXIHS010000000
VERSION     DAXIHS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.036"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..575
                     /locus_tag="ACFOF2_001081"
     CDS             <1..575
                     /locus_tag="ACFOF2_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC7107416.1"
                     /translation="DLGKLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITAT
                     AQTALAVAEAAGTVWRGKKVELNPTKWDWVKNNGYKTPAARPMQTLDGEMAGGNKPVV
                     KSIRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPNGEVIRTQRVPRT
                     DGVQGKYPQRQNYEGNPLPNNRHHSGYFIK"
     gene            572..889
                     /locus_tag="ACFOF2_001082"
     CDS             572..889
                     /locus_tag="ACFOF2_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107417.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            959..1252
                     /locus_tag="ACFOF2_001083"
                     /pseudo
     CDS             959..1252
                     /locus_tag="ACFOF2_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1247..1699
                     /locus_tag="ACFOF2_001084"
     CDS             1247..1699
                     /locus_tag="ACFOF2_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107418.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIETGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDRRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            1703..2104
                     /locus_tag="ACFOF2_001085"
     CDS             1703..2104
                     /locus_tag="ACFOF2_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107419.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            2263..2605
                     /locus_tag="ACFOF2_001086"
                     /pseudo
     CDS             2263..2605
                     /locus_tag="ACFOF2_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2639..2740
                     /locus_tag="ACFOF2_001087"
     CDS             2639..2740
                     /locus_tag="ACFOF2_001087"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107420.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNKLISGT"
     gene            2745..3101
                     /locus_tag="ACFOF2_001088"
     CDS             2745..3101
                     /locus_tag="ACFOF2_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC7107421.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            3149..3277
                     /locus_tag="ACFOF2_001089"
     CDS             3149..3277
                     /locus_tag="ACFOF2_001089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107422.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            3274..3648
                     /locus_tag="ACFOF2_001090"
                     /pseudo
     CDS             3274..3648
                     /locus_tag="ACFOF2_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3686..3948)
                     /locus_tag="ACFOF2_001091"
                     /pseudo
     CDS             complement(3686..3948)
                     /locus_tag="ACFOF2_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4028..4555)
                     /locus_tag="ACFOF2_001092"
                     /pseudo
     CDS             complement(4028..4555)
                     /locus_tag="ACFOF2_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(4604..5590)
                     /locus_tag="ACFOF2_001093"
     CDS             complement(4604..5590)
                     /locus_tag="ACFOF2_001093"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC7107423.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            complement(5630..6430)
                     /locus_tag="ACFOF2_001094"
     CDS             complement(5630..6430)
                     /locus_tag="ACFOF2_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC7107424.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLNTYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6555..7898
                     /gene="argG"
                     /locus_tag="ACFOF2_001095"
     CDS             6555..7898
                     /gene="argG"
                     /locus_tag="ACFOF2_001095"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC7107425.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            7973..8101
                     /locus_tag="ACFOF2_001096"
                     /pseudo
     CDS             7973..8101
                     /locus_tag="ACFOF2_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            8246..8374
                     /locus_tag="ACFOF2_001097"
                     /pseudo
     CDS             8246..8374
                     /locus_tag="ACFOF2_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            8367..8582
                     /locus_tag="ACFOF2_001098"
     CDS             8367..8582
                     /locus_tag="ACFOF2_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107426.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(8657..9937)
                     /locus_tag="ACFOF2_001099"
     CDS             complement(8657..9937)
                     /locus_tag="ACFOF2_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC7107427.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TKGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGTWTENSGGDVSGKF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(10049..11278)
                     /gene="sstT"
                     /locus_tag="ACFOF2_001100"
     CDS             complement(10049..11278)
                     /gene="sstT"
                     /locus_tag="ACFOF2_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC7107428.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            11807..13654
                     /locus_tag="ACFOF2_001101"
     CDS             11807..13654
                     /locus_tag="ACFOF2_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC7107429.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNTLENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLTGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMELKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            13709..17878
                     /locus_tag="ACFOF2_001102"
     CDS             13709..17878
                     /locus_tag="ACFOF2_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC7107430.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADCNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     HFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNVAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTRKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIDSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         4710 a         4837 c         4401 g         4234 t
ORIGIN      
        1 atgatttagg aaaattaagt ccggaagcac aacttgctgc cgcgagccta ttacaggaca
       61 gtgcttttgc ggtaaaagac ggcatcaatt ccgccagaca atgggctgat gcccatccga
      121 acattacagc tactgcccaa actgcccttg ccgtagcaga ggccgcaggt acggtttgga
      181 gaggtaaaaa agtagaactt aacccgacta aatgggattg ggttaaaaat aacggctata
      241 aaacacctgc tgcccgccct atgcagacgt tggacggtga gatggcagga ggaaacaagc
      301 cagttgttaa atctatcaga ccaactacgc gagatgaatt acgtcaagca ttgcaagaac
      361 aaggttttag acgtactggt tcagatgagg ctcaatatga aacatggaaa ggtcctgatg
      421 gcgtgaaaat agatattcgt ccaaatggag aggttataag aacccaaaga gtgccgcgaa
      481 ccgatggtgt acagggaaaa tatccgcaac gacaaaatta tgaaggcaat ccattgccaa
      541 ataatcgtca tcattctgga tattttatca aatgaaaaaa aatatttttc acaatgtaag
      601 cctttatgaa ataatctttt ccgataatgg aaataccctt acattatctt ttacagatac
      661 aattgaaggt aattatttcg gatatatcaa atgcagtaat attttgaatt ttaaattaga
      721 tacaaataat ttcgtagatt atgaggataa ggaagatagc ttgtttccct tgtttatacc
      781 cgaaatagag ctatataaat accaatttta tagtgaaatt attattgatg tagggattat
      841 tataaaaata tctgctgaaa caattaattt tgagccactg ggaaaatagt aactgctttc
      901 ccagcagccg tagcaactgt atttttaccc gacggggtaa aaatacagtt gctacatctc
      961 tgcgcagcag actctacaag atattaataa tttaggaaaa ttaagcccgg aagcacaact
     1021 tgctgccgcg agcatattac aggacagtgc ttttgcggta aaagacggca tcaattccgc
     1081 cagacaatgg gctgatgccc atccgaatat aacagcaaca gcccaaactg cccttgccgt
     1141 agcagaggcc gcaggtacgg tttggagagg taaaaaagta gaacttaacc cgaccaaatg
     1201 ggattgggtt aaaaataccg gctataaaaa acctgctgtt cgccatatgc agactaaggc
     1261 gttaggtacg gtagatgaaa ttggcgatac agtacagcag gttgggaaac aggctagcgg
     1321 acaaaaaacc agcggtggta atcctgcgat tgatagcgac ccctatagcc cgagtagtgt
     1381 ggcagctcgc atagaaaccg gtaaggtgcg cagtgattta caaatcaaag acattttgag
     1441 caatactact caaaggagta aaacaaaagg tcccgctgtt cagtatgata aagtggggga
     1501 ttacaatgac gcactaaatg attttaatag tctgaatgtt cgaaatgtac aaacacgtcc
     1561 taatggaacg ataacgggca atttacctga taggcgtgcg gttaatgctc gtaatgatag
     1621 tagtggtgga gaaccaacac ttgaaataac aattagtaat aaccgaaaaa taaaaatcag
     1681 atatggaaat acacgataaa ttatgaaatt aaaaagctta gatttcccaa ctggctattt
     1741 ctattttgat aatgcagcaa taaactctga taaagtagaa gttatagcag ttggttatag
     1801 aaatacggat aaaaccataa aaatttttat tgaagatgtt attcatttta gggttgttga
     1861 tgaatcgtat tttatagata cttttatgga tttaatttcg gaagatgcag atagagcttt
     1921 gcttcatgaa aatggtggtc aatctttttt tgaacttctt gatgagtgtt atgcggaatg
     1981 gatattgaaa gaaagttatt ttcctttgaa tagagaattc tttaaatact atatttttat
     2041 gtttgagcaa acattcatag aaataattgg ttctagtgca acgtattcaa ttattgaggg
     2101 ctagcgtaag atgagtaata agttgcctat ctttctttca ggcagcctga aaataaaact
     2161 acccaagttg atggtgtacc tgtatcagtg aagggaaatt ttgttgatgg taaatttcgc
     2221 attggtacgg caacaatgaa atcattttaa attgagctag aaatgaacct agaaaattat
     2281 gaaaacattt taataaaatt acttttttat cataacaact tagtaaatga atattcttat
     2341 tttattgaaa ataaaaattt atttaaaata gtatctcgaa ctaaaacgag taagggcttt
     2401 ttttcactat agaaaaacca ttaaattttc taagcaaaaa aacttatttt gagtttaatt
     2461 ttaaatattt acactcaggg aaagaacgct ttggttcgtt tatgtgctgg ataaatacta
     2521 atttaatgga atttgagggg gtttttttaa cgacctgctc cctgataata tgataataaa
     2581 taactttttt gaaataaatg attaacgata caccaataaa aattggtggg gtaaccgtat
     2641 ggatgatatt cgcggcatca tccaaggtgc ggttaatcct ttaatttaca aggtaagcaa
     2701 gcaaaagatt atttacaaca aactcatatc aggaacttag acaaatgaat gaacacaacc
     2761 tgttaatttt ctgtttaaaa gacaatgttt caattagtga atatactgaa atgattgatt
     2821 gggcttataa aaatattcaa tctgaaacag ttgtagaaat tacggaaagt caaattattg
     2881 aatatcaaaa tcgtggatta tggagacttg tttctgaaat taccgataat tggttatttg
     2941 gaccaagtga gggggattgg ctaatagata aggaaagtat tttggctgta aaagaaaaat
     3001 tacaaaattc agatttttct acagagccct tagtgaaaaa tattattcat gtacttgaat
     3061 atgctataaa aaataaaaaa acagtaattt ttcatttttg agactaatcc aatttttagt
     3121 aatattgatg cagagcaagc agcattagat gccgcaaaca tggggagaag ctattcaatt
     3181 tagaattaaa aaacaaattg aaaatgaact agcaccacca aattggtcta cccagtttcc
     3241 taatggtagt atttatgatc ctaaggtaac gaaatgatta ttcaaaatga atttaattta
     3301 tatcctagta atatgcttcc tgaaaggttt tgttatcctg aaaagtatgt tcgtatctct
     3361 aacgatacat ctttaatacc ttatattcag ccacataatt ttcactggta gtttgagaat
     3421 tatggaacag aaggggcaga agtagcttat atatttagaa attctatcct gcctgattta
     3481 aatcttatcc cattcgctag taatggagaa tgggaagctt attttgatgg taatgatgta
     3541 acaggaaatt ctagggttat tgtcattaat ttagataata tagaaaacca tgaatttttt
     3601 aatagttttg aagattggct tgaattagca attaaggata cttggtaagc agctatctat
     3661 aaagagatga ggctgccttg gacaactagg ataaactcga ttttactaat tgttttaaaa
     3721 tggaacaaga acttttattt cactgttgtt aaaacgccat tcgcactcct ttaaatacag
     3781 ctcaaaatgc gctttgggaa tgccgttaaa cttgcgtaaa tgacgttttg cttgattcca
     3841 aaagttctca gttccattaa tatggttttg tcgttcggca aaatgtgtgc tgtgattgat
     3901 acggaaatgg ctaaattcgc ccgcatccaa tacatcatag ccacgataac aaaatgagtt
     3961 tattttgttt ataccgtctt agacgacttt ctctcatagg gataattcta acttaatttg
     4021 aatttcccta gtgatctagg gcagccccta aattaataaa gcagcacaac tccttttgcc
     4081 gatgttccgg actgtcaaac gactgttcct catgccacat ctccatcaag gtacggataa
     4141 cccgctccgc cttaccgttg gtctgcggac aagcaaatcg ggcaagcctc caaccaatcc
     4201 cattattata acaagctgca ccgaaagcat gttggacggc tctttatatt acctatcatt
     4261 gtcagagtaa acgtactcaa tcaggtacaa gcaggggtca gccagatgtt cggtcagaaa
     4321 cttggcagca ctgtctgcgg ttttgtccgg caaaatggca gagtataaaa atcgtcaata
     4381 gcgacaaaca ggtaatctcg tttatcggcg gccttctgtc ctttgagcaa caacaaccga
     4441 tcggtatcag gatgaacaaa acctcccggg acaacctgcc ttttacggct ttaagtgcac
     4501 ggtaaatagt gacgcggctg acttagtggc agcatactgg ggaggtgagt gtttttgtgt
     4561 atatttttat tttggtattc ccttagaaat actgtaaaca acgctaccgg acggcctgca
     4621 gggcttcgcg cacgcttgct ttgagttctg cgccgaagcg tctgcccaag attctgccga
     4681 aatcgtcctt cggagtgtaa tccaccacat cgggggcttt gaccacgtct cgcgccacgc
     4741 tgtaaatatt gccgagtccg tccaccagcc cgactttcag cgcatccgcg cctgtgtaca
     4801 cgcgaccgct gaacacgtcg ggatattgtc ggaatttgag gcggccgccg cgtccggttt
     4861 tgacggcttt gatgaactca ccgtgtatgc cggtcagcat ttcttcccag atttttgact
     4921 gttcgggcgt ttcgggcgaa aacggatcgc ccatgccttt gttgctgcct gcaattttaa
     4981 ccctgcgttt cacgccgatt ttttccatca ggccggtcgc gtcgaaactg ctgccgataa
     5041 cgccgatgct gccgacgatg ctggacgggt cggcatagat tttgtccgcc gccgccgcga
     5101 tgtagtagca gccggacgcg cacatatctt ccgccacgag ataaacggga atgccggggt
     5161 gctgcgcctt cagacggcgt atttcttcaa aagcggtgtt ggagacgacg ggcgaaccgc
     5221 cggggctgtt ggcgcggatg acgatggctt ttgcctgcgg gtttttgtag gcggcctcca
     5281 taccgtcttt gagttttttg acctggtctt ctacaccgtt gccgatttcg ccgtacaggt
     5341 tgacgactgc ggtatgcggc gtgttgcccg ccaactgcaa tgcggcttcg tcttttcgga
     5401 aaatgcctgc aatcagggca accagaatca gggtgctgac ggcgcgccag atgtttttcc
     5461 acatccgctc cctgcgcctg tcctgatagg cggacagcag cacttcgcgc atgatgtcgc
     5521 gctcccataa ggtttccccc gcattttttg cttcgggtgc ttcgttttct cttctgattc
     5581 ggtattgcat ggttttcctt aaatattgtc cgatttgggc aaacggtttt cagtttaccc
     5641 gatttttcag ctctgctccc aatccgtcca agctgtgcaa cacttccgcc cacgccgcgt
     5701 ccaaaaggtt gacggcttct ccttcggctt tgatgccgaa ctcaatgtgc ggtttgacct
     5761 gcgtgccgtc tgaatgcgtc caaccgacgc tgggcaggct gtacgaacgc acaccgggat
     5821 aagtttgctc gatatgctcc ataagcggcg taatgcgcga ttcgggctgc tcaaacacat
     5881 acacgctgcg gctgccgcgt tcggtttggt tgaagcggtc ggcgtaataa gtattcaaaa
     5941 cccattccgc catcgggtgc gccatcacag gaaagccggg gaagaaataa tgctcgcgga
     6001 tggaaaatcc ggcgatgttg ttgaacgggt tgggcacaag ctctgcgccc tcggggaaat
     6061 ccgccatttt caggcgttgg gcgtgttccg gcgaatcaag cggctcgccg cgtttctggg
     6121 ttatgccttc gataaacttg gcggcttcag gatggcggac gacaggcaaa tccaaagcag
     6181 cagccgcggc ttggcgcgtg tggtcgtcgg gcgtggcgcc gatgccgccg gtaacaaacg
     6241 tcggtatgcc gtctgaaaag ctgcggcgca gttgcctgac cagtaaatcg ggttcgtcgg
     6301 gtagatattg cacctgattg agcttcagcc ctttggattc gagcagggat ttgaaaaagg
     6361 cgaaatgctt gtcttggcgg ctgccgtgca ggatttcgtc gccgatgatg atgaggttga
     6421 acgcgttcat aatgttttcg gttttaaata ccgcctgaaa cggcggaata cccgttcaga
     6481 cggcatgatg gctgacaata tttcctattt ataagataat ggctttgatt tttcaagcta
     6541 ccgaggattt tttcatgaac caaaaccata ctattttaca aaatctcccc gtcggtcaga
     6601 aagtcggcat cgccttctcc ggcggacttg atacctccgc cgcgctgttg tggatgaaac
     6661 tcaaaggcgc gctgccttat gcctacactg ccaacctcgg ccagcccgac gaagacgatt
     6721 acaacgccat tcccaaaaaa gcgatggaat acggcgcgga aaacgcccgc ttaatcgact
     6781 gccgcgcgca gttggcacat gaaggcatcg ccgccatcca atgcggcgcg ttccacgttt
     6841 ccaccggcgg catcgcctat ttcaacacca cgcctctggg ccgcgccgtg accggcacca
     6901 tgctcgtttc cgcaatgaaa gaagacgatg tgaatatttg gggcgacggc agcacctaca
     6961 aaggcaacga catcgagcgt ttctaccgct acggtttgct caccaatccc gcgctgaaaa
     7021 tctacaaacc ctggctcgat cagcaattta tcgacgaact cggcggccgt cacgaaatga
     7081 gcgaatttct gattgccaac ggcttcaact acaaaatgtc ggtggaaaaa gcctactcca
     7141 ccgattccaa tatgttgggt gccacccacg aagccaaaga cttggaattt ttgaactcgg
     7201 gcatcaaaat cgtcaaaccc attatgggcg ttgccttttg ggacgaaaac gtcgaaatcg
     7261 aacccgaaga agtcagcgta cgctttgaag aaggcgtgcc ggttgcactg aacggcaaag
     7321 aatacgctga tcccgtcgaa ctcttcctcg aagccaaccg catcggcggc cgccacggtc
     7381 tgggtatgag cgaccaaatc gaaaaccgca tcatcgaagc caaatcgcgc ggcatctacg
     7441 aagccccggg aatggcgttg ttccacatcg cctacgaacg cttggtaacc ggcatccaca
     7501 acgaggacac catcgaacaa taccgcatca acggcctgcg cctcggacgt ttgctctacc
     7561 aaggccgctg gttcgacagc caagccctga tgttgcgcga aaccgcccaa cgctgggtcg
     7621 ctaaagccgt taccggcgaa gttaccctcg aactgcggcg cggcaacgac tactcgattc
     7681 tgaacaccga atcgcccaat ctgacctacc aacccgaacg cctgagtatg gaaaaagtcg
     7741 aaggtgcggc gtttaccccg ctagaccgca tcggacagct cacgatgcgc aaccttgaca
     7801 tcaccgacac ccgcgccaaa ctgggtatct actcgcaaag cggtttgctc tcgctgggcg
     7861 aaggctcggt attgccgcag ttgggcaata agaaataagg tttgctgttt tgcatcatta
     7921 gcaacttaag gggtcgtctg aaaagatgat cccttatgtt aaaaggaatc ctatgaaaga
     7981 atacaaagtc gtcatttatc aggaaagcct gttgtccagc ctgtttttcg gcgcgacaaa
     8041 ggtcaacccc gtcaatttca gcgcgttcct caacaaacaa acccccgaag gctggcgggt
     8101 cgagaccttt gcaataacat aggttactaa aattttatgc tcaatctcat tttcaaaatg
     8161 caaaactttt ctgatttttc ctactttttg ctcaatatta ggaaggtttt aggcaattga
     8221 aaattttttg gcgcattttt atgcgtcaaa tttcgttaac agactatttt tgcaaaggtc
     8281 tcgggtcgta acgatggaaa aagatttgcg ccgtatgctg ctgtttttca aacgcgaggc
     8341 ctacgtcgtc attttggagc gggaccgtgt ttaagctcgg cgtttatgcc tgtctcggac
     8401 tgtttgccgg ctgggtgctg ctgctgattg tgcaactttg gttttctttt ctcgaagcgg
     8461 aattgttttt caaaatcact ctgactatgg cgggactgtt tgtcatcatc ctcgctgccc
     8521 tgctggtatt cggccagtat ttttccgaaa agaaaatgaa agacgacggg tttatcaact
     8581 gatgcggact tgaaccggac cccaccccaa acatcacaat gccgtctgaa cgcccccgct
     8641 tcaggcggca tcagcatcaa tcctgctctt ttttgccggc aaacacgccg aatccgccct
     8701 tttccgcatc tgtcgggcga tagctgtatt ttcccgccac ttcttcgccg gccgggccgt
     8761 aaaactttcc ggaaacatcc ccgccgctat tttccgtcca agtcccctta aagccgtttc
     8821 catcgatggc ggctttgaat ttttgcttac ccatatgcaa atcatcgccg ctgtcgataa
     8881 tgccgtccac agatttgctg ccgaaatcga cttttgcggc aaacctgcct ttggtcgggt
     8941 acgaacgtcc gttttccgta tggaaatgca gcacttcgcc gttgtacacg gccgtgccag
     9001 caagcatttc gccttttgcc ggttcgcctt gcacactgag ggcatacgat ccgccggaca
     9061 atttttctgc cccgtaagtc agataccggt aattcccttc gggcgcgaag atattgccgg
     9121 aatgccccgt caggctgacc gcttccccat cgacaatcag cgtatccgcc tgattgacgg
     9181 ggatcagcgg catctcagcc ggaagcgacc tcctagaccg tgcagaacgc ctgaatcgcg
     9241 cagatgaagt ggttttaggt ttgtaaaaaa taatatattg attggttcct ttcatctgta
     9301 acctatcagc aaccaaaccg gtaaactttt ctccatcttt tttatatttc ttaattttgt
     9361 cttcatcact taattgttca aattctgatt ttgacggtgc ttcttcgctt aatagattat
     9421 caccattaca agaatcgctt ttacaatggg tcaacgttat attttgcgac ggtccgtcaa
     9481 tcaaaacacc gttagccaaa tcaacccttc caaaattgcc accattcgtg gcattaggat
     9541 tgcttgaagg ggcaggattt tgagagccgc cgacttgatt gttttcggct tgatttgtac
     9601 cttgggcagc cgtattgccg gcattttgcg gcatatcatt ttgcggtccc tcgtcttcat
     9661 ttttgggttt gtccgttgtt gccgcaccgc cattgcctgt attttctgcc gaaactgccg
     9721 ccatatcttg gctgccttgt gtggatggcg cgccctgtcc ttgagaacct gcctgtggcg
     9781 catcttcttt tacctctgtc tctttttcag caacaacagg agcggccggt tttgacggtg
     9841 tgtccgccga cttaacatcg ggcgatccgc caccgccgcc cccacaggct gaaagggcaa
     9901 aaatacaagc cattgcaatc acactgcgtt caaacatcat catctccttc atattcaagg
     9961 cagccggtat tgtttccgcc atatgcccat aaaattgtaa aaatatgccg tctgaacgcc
    10021 aaatgggctt cagacggcat agcttggttt attccgcccg gtttctctgt cggcccaaat
    10081 cggcagcagc ggtaaacaaa acgtcggttg aagaattcaa cgccgtttcc gccgaatcct
    10141 gaatcacgcc gatgataaag ccgacggcaa ccacctgcat ggcgacatcg ttgctgatgc
    10201 cgaacaggct gcacgccagc ggaatcagca gcagcgaacc gcccgccacg ccggacgcgc
    10261 cgcacgcgct gaccgtcgcc accaggctca acagcagcgc ggtggcaaaa tcaacctgta
    10321 tgccttgggt gtgcgccgcc gccatagcca aaacggtaat cgtaatcgcc gcgccgccca
    10381 tattgacggt cgcccccaac gggatggaaa tcgaataagt gtcttcgtgc agccccagtt
    10441 ttttcgccaa agccatattc acggggatat tggcggcgga agaacgggta aagaaggcat
    10501 acacgccgct ttcgcgcagg caggtaaaca ccagcggata agggttgcgg cggattttcc
    10561 accacacgat ggcgggattg accactagcg cgataaacgc catacagccc aacagcaccg
    10621 caagcagctt cgcataaccc gccagcgcgc cgaaacccgt ctccgcgatt gtggacgaca
    10681 ccagcccgaa aatgcccaaa ggggcaaaac ggataatcca tttcacgacg gtggaaaccg
    10741 cttccgccaa atcggcaacg acctgccgcg taacgtccga accgtgattc cgcaacgccg
    10801 cgcccaaaac caaagcccaa gccagaatgc cgatatagtt ggcattggca atcgcgttaa
    10861 tcgggttggc gaccaggttc atcagcagcg atttcaacac ttccacaatg ccggaaggcg
    10921 gcgcggcgga cacatcgccc gcgcccgcca aaacaatgtg cgtcgggaaa accataccgg
    10981 cgatgacggc ggtcagggct gcggaaaacg taccgatgag gtaaaggacg ataatcggcc
    11041 tgatatgcgc cttgttgcct ttttggtgct gcgcgattgt ggccgccacc aaaataaata
    11101 ccaaaaccgg cgcgaccgct ttgagcgcgc cgacaaacag gctgccgaac aagcctgccg
    11161 ccaagcccag ttgcggggaa accgaaccga ttacgatgcc caacgccaaa ccggcggcaa
    11221 tctgcctgac caggctgacg cggccgatcg catgaaataa ggatttgccg aacgccataa
    11281 ttcttcctta tgttgtgata tgttaaaaaa tgttgtattt taaaagaaaa ctcattctct
    11341 gtgttttttt atttttcggc tgtgttttat agtggattaa caaaaatcag gacaaggcga
    11401 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttg agcaccttag
    11461 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
    11521 tgaaggttgc gttgatttgc cctatgcagt gccggacagg ctttgcttga tcattcggcg
    11581 caacggttta atttattgaa cgaaaataaa tttatttaat cctgcctatt ttccggcact
    11641 attccgaaac gcagcctgtt ttccatatgc ggattagaaa caaaatacct taaaacaagc
    11701 agatacattt ccggcgggcc gcaacctccg aaataccggc ggcagtatgc cgtctgaagc
    11761 gtcccgcccc gtccgaacag tgttaaaatc gaaatccgcc acaccgatgc acgacacccg
    11821 taccatgatg atcaaaccga ccgccctgct cctgccggct ttatttttct ttccgcacgc
    11881 atacgcgcct gccgccgacc tttccgaaaa caaggcggcg ggtttcgcat tgttcaaaaa
    11941 caaaagcccc gacaccgaat cagttaaatt aaaacccaaa ttccccgtcc gcatcgacac
    12001 gcaggatagt gaaatcaaag atatggtcga agaacacctg ccgctcatca cgcagcagca
    12061 ggaagaagta ttggacaagg aacagacggg cttcctcgcc gaagaagcac cggacaacgt
    12121 taaaaccatg ctccgcagca aaggctattt cagcagcaaa gtcagcctga cggaaaaaga
    12181 cggagcttat acggtacaca tcacaccggg cccgcgcacc aaaatcgcca acgtcggtgt
    12241 cgccatcctc ggcgacatcc tttcagacgg caacctcgcc gaatactacc gcaacacgct
    12301 ggaaaactgg cagcagccgg taggcagtga tttcgatcag gacagttggg aaaacagcaa
    12361 aacttccgtc ctcggcgcgg taacgcgcaa aggctacccg cttgccaagc tcggcaacac
    12421 ccgggcggcc gtcaaccccg ataccgccac cgtcgatttg aacgtcgtcg tggacagcgg
    12481 ccgccccatc gccttcggcg actttgaaat caccggcaca cagcgttacc ccgaacaaat
    12541 cgtctccggc ctggcgcgct tccaaccggg cacgccctac gacctcgacc tgctgctcga
    12601 cttccaacag gcactcgaac aaaacgggca ttattccggc gcgtccgtac aagccgactt
    12661 cgaccgtctc caaggcgacc gcgtccccgt caaagtcagc gtaaccgagg tcaaacgcca
    12721 caagctcgaa accggcatcc gcctcgattc ggaatacggt ttgggcggca aaatcgccta
    12781 cgactattac aacctcttca acaaaggcta tatcggctcg gtcgtctggg atatggacaa
    12841 atacgaaacc acgcttgccg ccggcatcag ccagccgcgc aactatcggg gcaactactg
    12901 gacaagcaac gtttcctaca accgttcgac cacccaaaac ctcgaaaaac gcgccttctc
    12961 cggcggcatc tggtatgtgc gcgaccgcgc gggcatcgat gccaggctgg gggcagagtt
    13021 tctcgcagaa ggccggaaaa tccccggctc ggatgtcgat ttgggcaaca gccacgccac
    13081 gatgctgacc gcctcttgga aacgccagct gctcaacaac gtgctgcacc ccgaaaacgg
    13141 ccattacctc gacggcaaaa tcggtacgac tttgggcaca ttcctgtcct ccaccgcgct
    13201 gatccgcacc tctgcccgcg caggttattt cttcacgccc gaaaacaaaa aactcggcac
    13261 gttcatcata cgcggacaag caggttacac cgttgcccgc gacaatgccg acgttccttc
    13321 ggggttgatg ttccgcagcg gcggcgcgtc ttccgtgcgc ggttacgaac tcgacagcat
    13381 cgggcttgcc ggcccgaacg gatcggtcct gcccgaacgc gccctcttga cgggcagcct
    13441 ggaataccaa ctgccgttta cgcgcaccct ttccggcgcg gtattccacg atatgggcga
    13501 tgccgccgcc aatttcaaac gtatggagct gaaacacggt tcgggactgg gcgtgcgctg
    13561 gttcagcccg ctcgcgccgt tttccttcga catcgcctac gggcacagcg ataagaaaat
    13621 ccgctggcac atcagcttgg gaacacgctt ctaaaccgat acggccgctt cagacggcat
    13681 tgcagcaaac tattttgaaa cagacattat gaccgatacc gcaccgacag ataccgatcc
    13741 gaccgaaaac ggcacgcgca aaatgccgtc tgaacaccgc cccaccccgc cggcaaaaaa
    13801 acgccgcccg ctgctgaagc tgtcggcggc actgctgtct gtcctgattt tggcagtatg
    13861 tttcctcggc tggctcgccg gcacggaagc gggtttgcgc ttcgggctgt accaaatccc
    13921 gtcttggttc ggcgtaaaca tttcctccca aaacctcaaa ggcacgctgc tcgacggctt
    13981 cgacggcgac aactggtcga tagaaaccga gggggcagac cttaaaatca gccgcttccg
    14041 cttcgcgtgg aaaccgtccg aactgatgcg ccgcagcctg cacattaccg aaatttccgc
    14101 cggcgacatc gccatcgtta ccaaaccgac tccgcctaaa gaagaacgcc cgccgctcag
    14161 ccttcccgac agcatagacc tgcctgccgc cgtctatctc gaccgcttcg agacgggcaa
    14221 aatcagcatg ggcaaagcct ttgacaaaca aaccgtctat ctcgaacggc tggatgcttc
    14281 ataccgttac gaccgcaaag gacaccgcct tgacctgaag gccgccgaca cgccgtggag
    14341 cagttcgtcg gggtcagcct cggtcggctt gaaaaaaccg tttgccctcg ataccgccat
    14401 ttacaccaaa ggcggactcg aaggcaaaac catacacagt acggctcggc tgagcggcag
    14461 cctgaaggat gtgcgcgccg aactggcgat cgacggcggc aatatccgcc tctcgggaaa
    14521 atccgtcatc cacccgtttg ccgaatcatt ggataaaaca ttggaagaag tactggtcaa
    14581 agggttcaac atcaatccgg ccgccttcgt gccttccctg cccgatgccg gactgaattt
    14641 cgacctgacc gccatcccgt cgttttcaga cggcatcgcg ctggaaggtt cgctcgattt
    14701 ggaaaacacc aaagccggct ttgccgactg caacggcatc cccgtccgtc aggttttagg
    14761 cggctttgtc atccggcagg acggcacggt gcatatcggc aatacgtccg ccgccctgct
    14821 cggacggggc ggcatcaggc tgtcgggcaa aatcgacacc gaaaaagaca tcctcgattt
    14881 aaatataggc atcaactccg tcggcgcgga agacgtactg caaaccgcgt tcaaaggcag
    14941 gttggacggc agcatcggca tcggtggcac gaccgcctcg cccaaaatct cttggcaact
    15001 cggcatcggc acggcgcgca cggacggcag cctcgccatt gcaagcgacc ccgcaaacgg
    15061 acagcggaaa ctggtgctcg acaccgtcaa catcgccgcc gggcaaggca gcctgaccgc
    15121 gcaaggctat ctcgagctgt ttaaagaccg cctgctcaag ctggacatcc gttcccgcgc
    15181 attcgaccct tcgcgcatca atccgcaact tccggcaggc aatatcaacg gctcaataaa
    15241 ccttgccggc gaactggcaa aagagaaatt cacaggcaaa atgcggtttt tacccggcac
    15301 gttcaacggc gtaccgattg ccggcagtgc cgacattgtt tacgagtccc gccaccttcc
    15361 gcgtgccgcc gtcgatttgc ggctggggcg gaacatcatc aaaacagacg gcggcttcgg
    15421 caaaaaaggc gaccggctta acctcaatat caccgcaccc gatttatccc gtttcggttt
    15481 cggactcgcg gggtctttaa atgtacgcgg acacctttcc ggcgatttgg acggcggcat
    15541 ccgaaccttt gaaaccgacc tttccggcgc ggcgcgcaac ctgcacatcg gcaaggcggc
    15601 agacatccgt tcgctcgatt tcaccctcaa aggttcgccc gacacaagcc gcccgatacg
    15661 cgccgacatc aaaggcagcc gcctttcgct gtcgggcgga gcggcggttg tcgataccgc
    15721 cgacctgatg ctggacggca cgggcgtgca gcaccgcatc cgcacacacg ccgccatgac
    15781 gctggatggc aaaccgttca aattcgattt ggacgcttca ggcggcatca acagggaact
    15841 tacccgatgg aagggcagca tcggcatcct cgacatcggc ggcgcattca acctcaagct
    15901 gcaaaaccgt atgacgctcg aagccggtgc ggaacgcgtg gcggcaagtg cggcaaattg
    15961 gcaggcaatg ggcggcagcc tcaacctgca acacttttcc tgggacagga aaaccggcat
    16021 atcggcaaaa ggcggcgcac gcggtctgca tatcgccgag ttgcacaatt tcttcaaacc
    16081 gcccttcgaa cacaatctgg ttttaaacgg cgactgggat gtcgcctacg ggcgcaacgc
    16141 gcgcggctac ctcaatatca gccggcaaag cggcgatgcc gtattgcccg gcgggcaggc
    16201 tttgggtttg aacgcatttt ccctgaaaac gcgctttcaa aacgaccgca tcggaatcct
    16261 gcttgacggc ggcgcgcatt tcgggcggat taacgccgat ttggacatcg gcaacgcctt
    16321 cggcggcaat atggcaaatg caccgctcgg cggcaggatt accgcctccc ttcccgactt
    16381 gggcgcattg aagccctttc tgcccgccgc cgcgcaaaac attaccggca gcctgaatgc
    16441 cgccgcgcaa atcggcggac gggtcggctc tccgtccgtc aatgtcgccg tcaacggcag
    16501 cagcaactac gggaaaatca acggcaacat caccgtcggg caaagccgct ctttcgatac
    16561 cgcgcctttg ggcggcaggc tcaacctgac cgttgccgat gccgaagtat tccgcaactt
    16621 cctgcccgtc ggacaaaccg tcaaaggcag cctgaatgcc gccgtaaccc tcggcggcag
    16681 catcgccgac ccgcacttgg gcggcagcat caacggcgac aagctctatt accgcaacca
    16741 aacccaaggc atcatcttgg acaacggttc gctgcgttcg catatcgcag gcaggaaatg
    16801 ggtaatcgac agcctgaaat tccgtcacga agggacggcg gaactctccg gtacggtcgg
    16861 tatggaaaac agcggacccg atgtcgatat cggcgcggtg ttcgacaaat accgcatcct
    16921 gtcccgcccc aaccgccgcc tgacggtttc cggcaacacc cgcctgcgct attcgccgca
    16981 aaaaggcata tccgttaccg ggatgattaa aacggatcag gggctgttcg gttcgcaaaa
    17041 atcctcgatg ccgtccgtcg gcgacgatgt cgtcgtatta ggcgaagtca aaaaagaggc
    17101 ggcggcaccg ctccccgtca atatgaacct gactttagac ctcaatgacg gcatccgctt
    17161 cgccggctac ggcgcggacg ttaccatagg cggcaaactg accctgaccg cccaatcggg
    17221 cggaagcgtg cggggcgtgg gcacggtccg cgtcatcaaa gggcgttata aggcatacgg
    17281 gcaggatttg gacattacca aaggcacggt ctcctttgtc ggcccgctca acgaccccaa
    17341 cctcaacatc cgcgccgaac gccgcctttc ccccgtcggt gcgggcgtgg aaatattggg
    17401 cagcctcaac agcccgcgca ttacgctgac ggcaaacgaa ccgatgagtg aaaaagacaa
    17461 gctctcttgg ctcatcctca accgcgccgg cagcggcagc agcggcgaca atgccgccct
    17521 gtccgcagcc gcaggcgcgc tgcttgccgg gcaaatcaac gaccgcatcg ggctggtgga
    17581 tgatttgggc tttacccgca agcgcagccg caacgcgcaa accggcgaac tcaaccccgc
    17641 cgaacaggtg ctgaccgtcg gcaaacaact gaccggcaaa ctctacatcg gctacgaata
    17701 cagcatctcc agcgcggaac agtccgtcaa actgatttac cggctgaccc gcgccataca
    17761 ggcggttgcc cgtatcgaca gccgttcgtc gggcggcgag ctgacataca ccatacgttt
    17821 cgaccgcttc tccggttcgg acaaaaaaga ctccgccgga aacggcaaag gaaaataagc
    17881 ggttttcaga cggcgcgccg ccaaaccgga catttgaaaa cctgcttttc caccgtccgc
    17941 cgccgccgtc cgcctgcaag ggaacagaat cgatatagtg aattaacaaa aatcaggata
    18001 aggcgacgaa gccgcagaca gtacaaatag tacggaaccg attcactcgg tgcttgagca
    18061 ccttagagaa tcgttctctt tgagccaagg cgaggcaacg ccgtaccggt ttttgttaat
    18121 ccgctatatt ccgccatctc taagatttac agcgatacac aggtaattta aggaatgccc
    18181 ga
//
LOCUS       DAXIHS010000037        17958 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXIHS010000037 DAXIHS010000000
VERSION     DAXIHS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.037"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="ACFOF2_001103"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="ACFOF2_001103"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC7107431.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIVIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFASHTPQNYGDMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1394..1777)
                     /locus_tag="ACFOF2_001104"
     CDS             complement(1394..1777)
                     /locus_tag="ACFOF2_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC7107432.1"
                     /translation="MYCLRLRRLVLIFVNPLYQTEEGIAQTVVKGKTTRAEVEARFGK
                     RNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKD
                     ERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            complement(1880..1960)
                     /locus_tag="ACFOF2_001105"
                     /pseudo
     CDS             complement(1880..1960)
                     /locus_tag="ACFOF2_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2163..3491
                     /gene="miaB"
                     /locus_tag="ACFOF2_001106"
     CDS             2163..3491
                     /gene="miaB"
                     /locus_tag="ACFOF2_001106"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC7107433.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(3576..5489)
                     /gene="dxs"
                     /locus_tag="ACFOF2_001107"
     CDS             complement(3576..5489)
                     /gene="dxs"
                     /locus_tag="ACFOF2_001107"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC7107434.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMELAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(5574..6491)
                     /gene="xerC"
                     /locus_tag="ACFOF2_001108"
     CDS             complement(5574..6491)
                     /gene="xerC"
                     /locus_tag="ACFOF2_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC7107435.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6685..7749
                     /gene="fba"
                     /locus_tag="ACFOF2_001109"
     CDS             6685..7749
                     /gene="fba"
                     /locus_tag="ACFOF2_001109"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC7107436.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            7907..8728
                     /locus_tag="ACFOF2_001110"
     CDS             7907..8728
                     /locus_tag="ACFOF2_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC7107437.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTTPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQTITLASGEFQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPDGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKA
                     DEKSHAVILGDTRYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            8867..9544
                     /gene="tsaB"
                     /locus_tag="ACFOF2_001111"
     CDS             8867..9544
                     /gene="tsaB"
                     /locus_tag="ACFOF2_001111"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC7107438.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRISIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            9541..9981
                     /gene="rimI"
                     /locus_tag="ACFOF2_001112"
     CDS             9541..9981
                     /gene="rimI"
                     /locus_tag="ACFOF2_001112"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC7107439.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            9975..10712
                     /locus_tag="ACFOF2_001113"
     CDS             9975..10712
                     /locus_tag="ACFOF2_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC7107440.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            10774..11415
                     /gene="pyrE"
                     /locus_tag="ACFOF2_001114"
     CDS             10774..11415
                     /gene="pyrE"
                     /locus_tag="ACFOF2_001114"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC7107441.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            11489..11923
                     /locus_tag="ACFOF2_001115"
     CDS             11489..11923
                     /locus_tag="ACFOF2_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC7107442.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            11920..13230
                     /gene="argA"
                     /locus_tag="ACFOF2_001116"
     CDS             11920..13230
                     /gene="argA"
                     /locus_tag="ACFOF2_001116"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC7107443.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCATLKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            13326..15341
                     /locus_tag="ACFOF2_001117"
     CDS             13326..15341
                     /locus_tag="ACFOF2_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC7107444.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWGERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLALFAL
                     DLNVMELTVMRRQPQQFDSDGINVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(15455..16411)
                     /locus_tag="ACFOF2_001118"
     CDS             complement(15455..16411)
                     /locus_tag="ACFOF2_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC7107445.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     AFIESGSTVQAAMHFCGYRHAGRLNEAFRRHYGFLPSDAKKC"
     gene            16647..17612
                     /locus_tag="ACFOF2_001119"
     CDS             16647..17612
                     /locus_tag="ACFOF2_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7107446.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         4002 a         5432 c         4892 g         3632 t
ORIGIN      
        1 caatctgcca aacaaattga acagggaaac cctgtccgtc aaaacatcat gcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcatcaat cagctcgggc gtatgcgcgg
      121 cagaaacgaa acctgcttca taagcggacg ggccgaaggc aatgccgcgg tcgagcatgc
      181 cgtggaaaaa ctgtttgaag ccttcaatat tggaacgcgc catgtcgccg tagttttgcg
      241 gcgtatggct ggcaaaatac aaaccaaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtctgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata aaacccttcg cggcggatga tttccagcgt tttcaaaccg gcggcgacgg
      421 caatcgggtt gcctgataat gtacctgcct gatacacgcc gcccagtggg gaaatacatt
      481 ccataatgtc tttgcgtccg ccgaacgcgg caagcggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcagcgattg tgcgccaccg agtgcgacgc
      601 ggaaacctgt catcacttcg tcgtaaatca acaccgcgcc gtgtttttca gtcaattcgc
      661 gcagggcttt gacgaaggct tcggtcgggc ggacaaggtt catattgccg acaaaaggct
      721 ccaaaatcac acaggcaatg tcattgccgc tttgagcaaa ggcttcttcc aattgggcga
      781 tgttgttgta ttcgaggact aaggtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 aaggattgcc gaacgtcagc aggccgctgc cggctttcac cagcaggctg tcggaatgcc
      901 cgtggtagca gccttcaaac ttgatgattt tgtcgcggcc ggtaaaaccg cgtgccagac
      961 ggatggcggt catcgtcgct tccgtgccgg agctgacgag gcgcagccgt tcgacagacg
     1021 gcataatttc ggcgatttgt tcggcgatga cgatttcgcc ctcggtgggc gcaccgaatg
     1081 acaaaccgcc caacgcggct tcgcgcacgg cttcgacgac ttcggggtgc gcgtgtccga
     1141 caatcgcagg gccccaagag ccgacatagt cggtgtagcg cgtgccgttt tcatcccaaa
     1201 catacgcgcc ttcggctttt ttgatgaagc gcggtacgcc gccgacgctg ccgaatgcgc
     1261 gcacggggga attcacgccg ccggggatga ccgccttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atttatcctc aaatgccgtc tgaacggcag gtttcgggct tagaagcaga
     1381 aagtcccatt ttatcatttt tcaggttgcg acaaggattt gcctgcttct ttgcggatga
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtcccggac aactccttcc cagtcgtaat ccacacacca ctcccaatgt cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acctcgtgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacctcc gcccgggtcg
     1681 ttttgccttt aacaaccgtt tgtgcgattc cctcttccgt ctgatatagt ggattaacaa
     1741 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggaacc gattcacttg
     1801 gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg
     1861 tttttgttaa tccactataa cgcaggaact gatgttccct gtcgccgaaa ttgctggtac
     1921 acgcacacag cagcaatgcc gcccacacag ccggtttcat acacacctcc cattaaagcc
     1981 aaacattata tagccgtccc gaccgattaa attcatattt taaaacaata ttctgtttac
     2041 aaaacccaca tcgtgctata atccgcaccg attttcagac ggcatcgtca tgccgtctga
     2101 aattttttca ttccaacaac aatcagcccc gcgattacgg ctgcctgaga aagacacaaa
     2161 ccatgaaaaa agtatttatc cgcaccttcg gctgccagat gaacgaatac gacagcgaca
     2221 aaatgctcgc cgtcctcgcc gaagaacacg gcggcatcga acaggttacc caagccgacg
     2281 aagccgacat catcttgttc aacacctgct ccgtgcgcga aaaagcgcaa gaaaaagtct
     2341 tctccgattt ggggcgcgtg cgtccgctca aagaaaaaaa ccccggcctc atcatcggcg
     2401 ttgccggctg cgtcgcctcg caagaaggcg aaaacatcat caaacgcgcg ccttatgtgg
     2461 acgtggtttt cggcccgcaa acgctgcacc gcctgccaaa aatgattgtg gacaaagaaa
     2521 ccagcgggct gtcgcaagtc gatatttcct tccccgaaat cgaaaaattc gaccacctgc
     2581 cgcccgcccg cgtcgaaggc ggcgcggcat ttgtatcgat tatggaaggc tgttccaaat
     2641 actgctcctt ctgcgtcgtc ccctacacgc gcggcgaaga attctcccgc ccgctcaacg
     2701 acgtattgac agaaatcgcc aaccttgccc agcaaggcgt gaaagaaatc aacctcttgg
     2761 gacaaaacgt caacgcctat cgcggcgaaa tggacgacgg cgaaatctgc gacttcgcca
     2821 ccctgctgcg catcgtccac gaaatccccg gcattgaacg tatgcgcttc accaccagcc
     2881 acccgcgcga gtttaccgac tcgattatcg agtgctaccg cgacctgccc aaattggttt
     2941 cccacctgca cctgccgatt caaagcggtt ccgaccgcgt attgagcgcg atgaaacgcg
     3001 gctacaccgc tttggaatac aaatccatca tccgcaaact gcgtgccatc cgtcctgatt
     3061 tgtgcctgag cagcgatttc atcgtcggct tccccggcga gaccgaacgc gagttcgagc
     3121 aaaccttgaa actggtgaaa gacatcgcct tcgacttgag cttcgtgttt atttacagtc
     3181 cgcgccccgg cacgcctgcc gccaacctgc cggacgacac gccgcacgaa gaaaaagtgc
     3241 gccgcctcga agccttgaac gaagtcatcg aagccgaaac cgcgcgcatc aaccaaacga
     3301 tgatcggcac ggttcaacgc tgcctggtcg aaggcatctc caaaaaagac cccgaccagc
     3361 tccaagcccg caccgccaac aaccgcgtcg tcaactttac cggcacgccc gacatgatta
     3421 accaaatgat cgatttggaa atcaccgagg cctacacctt ctccctgcgc ggcaaagttg
     3481 tcgaagccta aacccttacg ccgaaaaaat gccgtctgaa gcgtttcaga cggcattttg
     3541 ccttgtatcg gcagacgatg gcgcggctag gcggcttaat ttgccgcatc ccgatccgac
     3601 aaccacgcgc gcacacgccg ttccaccgct tcggcactca agcccaaatc gtctaaaagt
     3661 tttttcggat cgccgtgtcc ggttacggta tcggcaacgc ccaaaagcaa aacgggtttg
     3721 cagatgccgt gtttcgccaa tacttccagc accgcgccgc ctgcgccgcc ctgttcggcg
     3781 ttttcttcaa gggtaacgat gcggtcgtgg cttcgggcaa ggcggacaat caactcttcg
     3841 tctatcggtt tgacgaagcg catatcggcg acggtggcgt tcagtttttc ggcaaccgcc
     3901 aatgcggggg cgaccatact gccgaaggca atgaaggcgg ttttcccacc ttcgcggcgg
     3961 ataatgccct tgccgatttc cacggtttcc atgccgtctg aaaccggcgc gcccgtaccc
     4021 gtgccgcgcg gatagcggac ggcggcgggc gcatccgcct gatagcaggt cgaaagcagc
     4081 aggcggcatt cgttttcatc gctcggcgcg gcgacaatca tattcggaat gcagcgcaaa
     4141 aagctcaaat cgtacagacc ggcatgggtc gggccgtccg cgccgacgat gcccgcgcgg
     4201 tcgacggcaa acaaaacggg caggttttgc agggcgatgt cgtgcaccag ttggtcgtag
     4261 gcgcgttgta aaaaggtgga ataaatcgcc acgacgggct tcatgccttc gcacgccaaa
     4321 ccgccggcaa aggtaacggc gtgctgctcg gcaatgccga catcgaaata acggtcgggg
     4381 aatcgttgtt caaactccac cagtccgctg ccctcgcgca tcgcgggcgt gatggcgatc
     4441 aaacgctcgt ctgccgccgc ccggtcgcac agccatttgc cgaacacttg ggtataggtc
     4501 ggtttggcgg cgggcttggg ttctttttca gacggcattt gcgccgcgct ttctttaggc
     4561 aggttggcga cggcgtggta tttgacggga tcgttttcgg cgagtttgta gccgttgccc
     4621 tttttggtga tgacgtgcag cagttgaggg cctttgcggc tgcgcaagtc tttcaatacg
     4681 tccaccagat tttcgacgtt gtgtccgtcc acggggccgg tgtagcggaa gccgaagttt
     4741 tcaaacaaag acagcgactg tttggcgtgt tcggcttctt cggcaagggt tttgattttg
     4801 tgttcgactt tttgggcgag ctccatcgcg ccgggtattt tgtctaatac cttgcccgtt
     4861 tgcgctttga cggtactcaa caggccgtgc atatcgcgca cgacgttgct ggcgaggtat
     4921 ttcggcagcg cgccgacgtt gggggaaatc gacatttcgt tgtcgttgag gacgaccagc
     4981 aaatccacat ccatatcgcc tgcgcaattc aaggcttcaa acgcctgccc cgccgtcatc
     5041 gcgccgtcgc cgatgatggc gacgctgcgg cggtcgctgc ccaagagttt gtctgccgcc
     5101 gccatgccca acgccgcgcc gatggatgtg gaagaatgcc ctacgccgaa cgcgtcgtat
     5161 tccgactcgc tgcgttttgg gaagccagct aaaccgccgt attggcgcat ggtgtgcatc
     5221 tggtttttcc tgcctgtcag gattttgtgc ggatagcttt ggtgtccgac atcccacacc
     5281 agcttgtctt cgggcgtgtc gtacacatag tgcagggcga tggtcagttc gaccgcgccc
     5341 aaattgctgg cgaaatgccc gccggtctgc ccgacagatt ccagcagaaa ggtgcgcaac
     5401 tcgccggcaa ggcgcggcag ctgttttttg tccagacggc gcaaatcttg cgggctgtca
     5461 atcaggtcga gtagggggct tgggttcatg gtgtgtcttt tttatgtgtc gtccaggtgc
     5521 aacgaccaat tatatatcaa gagcgtgcgg ctgacggctg attttgccgt atgtcattcg
     5581 tcctgccgct tggcgcgcgg gtgggcttcg tcatacaggc gggcgatgtg gtcgaaatcg
     5641 agtttggtgt aaatctgcgt ggtcgaaagg ctgctgtgcc cgagcagctc ctgcaccgcc
     5701 cggatgtcgc gcgaagcctg caacaggtgt ccggcgtagc tgtggcgcat catatgcggc
     5761 gaaacgtgcc tgccgtcgcc gttttgcgcc gcccattgcg ccaaacgttt ttggatttgg
     5821 cgttggctca ggcgcgtgcc gttcctgccg gtaaacaggg ctttgccgtc cgatgccgtc
     5881 tgacgcagcg gcagatagtt tttcagggct tccacgcttt tgccgaccag cggcacctgc
     5941 cgctgcttgc gccctttgcc ggtaacgtgt acccacgcct cgtccaaata gacatcatct
     6001 gcattcaagc cgtgtatctc gctcacgcgc aaaccgctgc cgtacatcag ctcgaacagg
     6061 gcgtgatcgc gcaccgccag cgggtcgccg ccgtccacgg gcaaatccag catccggttc
     6121 agccattcct gcgacagggc tttgggtacg cgctcgggct gcttcggcgg tttgatgtcg
     6181 gcggtcgggt cggtatgcag cagaccgcgt tccaccagcc aaacacaata ctgccgccaa
     6241 gacgacagct tgcgcgccag cgtccgcgca ttcaaaccgc gctgcgacag ccgccgcaac
     6301 gccgccgtaa aatcgcgccg cgacaagtcc tgcggcacgc cgccttcatc ttcagacggc
     6361 atttgtgcca acagcgcaaa cagttcttcc aaatcgcgcc ggtatgccgc caccgtgtgc
     6421 tccgacttgc cctcgcgcac gatgttttcc aaataagcgt caaaatgcgc cgcaaacccg
     6481 tccaaaacca taaccgcccc acacaaatat caaaaaaaca gtgaaaacat tatcataaac
     6541 gacacaccaa aaccccgaac cgcccaaaat ccgacccgtc cgcccgccgt tatcgttaac
     6601 ataaaatcaa tgagccgttt atggtttttt gccgcaaaaa acattataat ccgccttatt
     6661 tacctattgc ccaaggagac acaaatggca ctcgtatcca tgcgccaact gcttgatcat
     6721 gctgccgaaa acagctacgg cctgcccgcg ttcaacgtca acaacctcga acaaatgcgc
     6781 gccattatgg aagccgccga ccaagtcaac gcgcccgtca tcgtacaggc gagtgcaggt
     6841 gcgcgcaaat atgcgggcgc gccgtttttg cgccacctga ttctggcggc tgtcgaagaa
     6901 tttccgcaca tccccgtcgt gatgcaccaa gaccacggcg catcgcccga cgtgtgccaa
     6961 cgctccatcc aactgggctt ttcctccgtg atgatggacg gctcgctgat ggaagacggc
     7021 aaaacccctt cttcttatga atacaacgtc aacgccaccc gtaccgtggt taatttctcc
     7081 cacgcttgcg gcgtatccgt cgaaggcgaa atcggcgtgt tgggcaacct cgaaaccggc
     7141 gaagccggcg aagaagacgg tgtaggcgca gtgggcaaac tttcccacga ccaaatgctc
     7201 accagcgtcg aagatgccgt gcgtttcgtt aaagataccg gcgttgacgc attggcgatt
     7261 gccgtcggca ccagccacgg cgcgtacaaa ttcacccgtc cgcccacagg cgacgtgttg
     7321 cgtatcgacc gcatcaaaga aatccaccaa gccctgccca atacacacat cgtgatgcac
     7381 ggctccagct ccgttccgca agaatggctg aaagtcatca acgaatacgg cggcaatatc
     7441 ggcgaaacct acggcgtgcc ggttgaagaa atcgtcgaag gcatcaaaca cggcgtgcgc
     7501 aaagtcaaca tcgacaccga cttgcgcctt gcttccaccg gcgcggtacg ccgctacctt
     7561 gccgaaaacc cgtccgactt cgatccgcgc aaatacctga gcaaaaccat tgaagcgatg
     7621 aagcaaatct gcctcgaccg ctatcttgcg ttcggttgcg aaggtcaggc aggcaaaatc
     7681 aaaccggttt ccttggaaaa aatggcaagc cgttatgcca agggcgaatt gaaccaaatc
     7741 gtcaaataac aggttgcttg taaacaaaat gccgtctgaa ccgttgttcg gacggcattt
     7801 gatttttgct tctttgacct gcctcattga tgcagtatgc aaaaaaagat accataacca
     7861 aaatgtttat atattatcta ttctgcgtat gactaggagc aaacctgtga accgaactgc
     7921 cttctgctgc ctttctttga ccgccgccct gattctgacc gcctgcagca gcggaggcgg
     7981 cggtgtcgcc gccgacatcg gcgcggggct tgccgatgca ctaaccacac cgctcgacca
     8041 taaagacaaa agtttgcagt ctttgacgct ggatcagtcc gtcaggaaaa acgagaaact
     8101 gaagctggcg gcacaaggtg cggaaaaaac ttatggaaac ggcgacagcc tcaatacggg
     8161 caaattgaag aacgacaagg tcagccgctt cgactttatc cgtcaaatcg aagtggacgg
     8221 acaaaccatc acgctggcaa gcggcgaatt tcaaatatac aaacagaacc actccgccgt
     8281 cgttgcccta cagattgaaa aaatcaacaa ccccgacaaa atcgacagcc tgataaacca
     8341 acgctccttc cttgtcagcg gtttgggcgg agaacatacc gccttcaacc aactgcctga
     8401 cggcaaagcc gagtatcacg gcaaagcatt cagctccgac gacccgaacg gcaggctgca
     8461 ctactccatt gattttacca aaaaacaggg ttacggcaga atcgaacacc tgaaaacgcc
     8521 cgagcagaat gtcgagcttg cctccgccga actcaaagca gatgaaaaat cacacgccgt
     8581 cattttgggc gacacgcgct acggcggcga agaaaaaggc acttaccacc tcgccctttt
     8641 cggcgaccgc gcccaagaaa tcgccggctc ggcaaccgtg aagataaggg aaaaggttca
     8701 cgaaatcggc atcgccggca aacagtagca tttgaaaaat gccgtccgaa cacgatagtt
     8761 taccgttcgg acggcatttt tgcgccatac tgccctagga cacgacggca cggcaggcaa
     8821 gccggtttca ctatataatg ccgtctgaac caacgagaac cacaccatgc aagccgattt
     8881 caaccgcccc gtcctcgccg tcgataccgg aacttcctat ttgtcgctcg cgctgcgcgc
     8941 cgacggcgaa accctcctgt tccatcagga ggccggcagc cgccagtccg aactgatcct
     9001 gccggaaatc cgcaccctat tccgaaatgc gggcattacc gccgccgatt tgggggcgat
     9061 tgtttatgcg aaaggtcccg gcgcgtttac cggactgcgt atcagcatcg gcgtggcgca
     9121 aggtttggca acgccattca atacgccttt aatcggcatc cccacgctcg atgccgccgc
     9181 ctcgctgccg ccgccgcaaa gctgcatact cgccgctgcg gacgcgcgta tgggcgaagt
     9241 gttttatgcg tggttcgaca cgctgaaccg ccgccgtttg agcgattatc aggtcgggcg
     9301 ggcggcagac atccggctgc cggagggatg tgccttttca gacggcatag gcagcgcgtt
     9361 cgcgttggaa gaaacgccgc cgttttcagg caaacccgat atgccgaccg ccgccgactt
     9421 cctcaatctg gcggcggaag gcggttttcc cgccgtcgct gctgcacact ccgaactgct
     9481 ctacgtccgc aacaaaatcg ccctaaccgc caaagaacag gcggaacgga aagcccgaac
     9541 atgaacatcc gccgcgcctc accgtccgac tgcgccgcac tcgccgcgct cgatgccgtc
     9601 tgcaacccgt ccgcgtggac gcaacgccaa tttgagtccg cattggtttc gccgtccgaa
     9661 caggttttcc tcgcggaaaa agacggcggg attgccgcct ttatcgtttg gcagaacctg
     9721 cccgacgaat ccgaactgca cctgattgcc accgcgcccg aatgccgccg ccgtggcgtt
     9781 gcgtccgccc tgctcgaata ttggttcgca catctgcccg aagacacgca acgcctgctg
     9841 ctcgaagtcc gtgcaggcaa caccgccgca caggcactgt acgcggcgca cggctttacc
     9901 gatgcgggca ggcggaaaaa ctattaccgt acagccgacg gtaaaaccga agatgctgtc
     9961 ttaatggaga aaatatgtta agcgcgcgct acctccacct gcacgaagct ttgggtttag
    10021 gcccgatgtg gctgaaacag gccgccgccg tcctgccgcc caaaaacaca cccgcaaccc
    10081 cggcacaggc acgtcctcaa accgtccgcg ccgccccgat ccgcccttcc caaccccata
    10141 acggtcaggc gcggctcgaa acgatgaaag cgttggaaac cgccgccgta catacgcgca
    10201 aacccgcgcc tgaaaccgaa acgcccccgc ccggcctttc agacggcatc gcccccgttc
    10261 ccgccgcttc gggcataacc aaacttgccg tcgtcagcct ttgcccaccg attgaggatg
    10321 cggtttacgg gcaactgttc tacggcaaag cgggtgttct gcttgacaac atactcaaag
    10381 ccgtaggact ggatgccgcc tatgtccaca aaacctgttg ggtgaaaacc gccgccgtcg
    10441 gcaacccgat gccgtctgaa gcggcaatcg aaaccgcgac ggtttccgtc atgcaggaac
    10501 tcgacggctg ccgcgccccc gccgtcctat tcctcggaca ggcttttgtc cagcctgaac
    10561 ggcaaacgat gattgaaact ttgtgcggca gccgtccctt cttcatcatc gaccatcccg
    10621 cccggctgtt gcgccaaccc gaacttaaag cccgcgcctg gcaggtgttg aaacagttga
    10681 aacgcgcctt gcggcaaggc ggcggcagtt gaagcgcacc gcacggggcg gtagaatcgc
    10741 aactgcgtcc caatatctga cagaaagcac aaaatgaccg atttccgcca agatttcctc
    10801 aaattctccc tcgcccaaaa tgttttgaaa ttcggcgaat ttaccaccaa agccggacgg
    10861 cggtcgccct atttcttcaa tgccggcctc tttaacgacg gcttgtccac gctgcaactg
    10921 gcaaaatttt acgcacaatc catcattgaa agcggcatcc gattcgatat gctgttcggc
    10981 cccgcctaca aaggcattat tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc
    11041 gtgaacgtcc cgtttgccta caaccgcaaa gaagccaaag accacggcga aggcggcgtg
    11101 ttggtcggtg cgccgcttaa aggccgcgtg ctgattatcg acgacgtgat ttccgccggc
    11161 acatccgtac gcgaatcgat caagctgatt gaagcggagg gcgcaacccc cgccggtgtc
    11221 gccatcgcgc tcgatcgcat ggaaaaaggc acgggtgagt tgagcgcggt tcaggaagtg
    11281 gaaaaacaat acggtctgcc cgtcgccccc atcgccagcc tgaacgattt gtttattctg
    11341 ttgcaaaaca accccgaatt cggacagttc ctcgaacccg tccgagccta ccgtcggcag
    11401 tacggcgtag aataaaaaca aagcatatgc cgtccgaacc gccttacgcc tcagacggca
    11461 tcaaacctga cacacacgag gaaataccat gcccgcctgt ttctgccccc actgcaaaac
    11521 ccgtctctgg gtcaaagaaa cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa
    11581 atgcgaagga ctgtttaaag ccaaagacca tctggcaagc acgaaagaac ccatattcaa
    11641 cgatttgccc gaggctgttt cggatgtcaa actcgttcac cgtatcggca cgcgcgccat
    11701 cggcaagaaa cagatttccc gtgacgaaat cgccggcatc ctcaacggcg gcacaaccca
    11761 gcccgatatt ccgcccgcaa ccgccgccac ccctgctgcc gcaccgcagg ttaccgtacc
    11821 gcccgccgcg cccgcccgtc aggatgggtt caactggacg attgcaaccc tgtttgccct
    11881 tatcgtcctc attatgcagc tttcctacct cgtcatccta tgatcgtgcc cgacctcttt
    11941 gtcgcccact tccgcgaagc cgccccctac atccgccaaa tgcgcggcaa aacgctggtc
    12001 gccggcatag acgaccgcct gctcgaaggt gataccttaa acaagctcgc cgccgacatc
    12061 gggcttttgt cgcaactggg catcaggctc gtcctcatcc acggcgcgcg ccacttcctc
    12121 gaccgccacg ccgccgcgca aggccgcacg ccgcattatt gccggggctt gcgcgttacc
    12181 gacgaaacct cgctcgaaca ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa
    12241 gccgcattgt gcggcagcgt ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg
    12301 ggcaacttcc tgaccgcccg tccgataggt gtgattgacg gaaccgatat ggaatacgcg
    12361 ggcgttatcc gcaaaaccga caccgccgcc ctccgtttcc aactcgacgc gggcaatatc
    12421 gtctggctgc cgccgctcgg acattcctac agcggcaaga ccttccatct cgatatgctt
    12481 caaaccgccg cctccgtcgc cgtctcgctt caggccgaaa aactcgttta cctgaccctt
    12541 tcagacggca tttcccgccc cgacggcacg ctcgccgtaa ccctctcggc acaggaagcg
    12601 caatcgctgg cggaacacgc cggcggcgaa acgcgacggc tgatttcgtc cgccgttgcc
    12661 gcgctcgaag gcggcgtgca tcgcgtccaa atcctcaacg gtgccgccga cggcagcctg
    12721 ctgcaagaac tcttcactcg caacggcatc ggcacgtcca ttgccaaaga agccttcgtc
    12781 tccatccggc aggcgcacag cggcgacatc ccgcacattg ccgccctcat ccgcccgctg
    12841 gaagaacaag gcatcctgct gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa
    12901 ttttccatcc tcgaacacga cggcaacctg tacggttgcg ccaccctaaa aacctttacc
    12961 gaagccgatt gcggcgaaat cgcctgcctt gccgtctcgc cgcaggcaca ggacggcggc
    13021 tacggcgaac gcctgcttgc ccacattatc gataaggcgc gcggcatagg cataagcagg
    13081 ctgttcgcac tgtccacaaa taccggcgaa tggtttgccg aacgcggctt tcagacggca
    13141 tcggaagacg agttgcccga aacgcggcgc aaagactacc gcagcaacgg acggaactcg
    13201 catattctgg tacgtcgcct gcaccgctga ccgcaacgga aagccgccgc agaaatgccg
    13261 tctgaacccc gtttcagacg gcatttcccc gattataaag acctgcccaa ccctcaagga
    13321 ccccgatgaa atcctacccc gacccctacc gccattttga agacctcgat tccgccgaaa
    13381 cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcgc gcgtttttta aacaacgaca
    13441 aggcgcgcgc attgtctgac ggtattttgg cgcagttgca ggacacgcgg cagattccgt
    13501 tttgtcagga acaccgcgcg cggatgtacc atttccatca ggacgcggaa tatccgaagg
    13561 gcgtgtaccg cgtgtgtacc gcggcgacat accgttccgg ctatcctgag tggaaaatcc
    13621 tgttttcagt ggcggatttc gacgaattgc tcggcgacga tgtatatttg ggcggcgtgt
    13681 cgcacttggt ggaacagccc aaccgtgcgt tgttaacact gagcaaatcg ggcggcgata
    13741 cggcgtacac gctggaagtg gatctggaag caggggagtt ggtagaaggc ggttttcact
    13801 ttccggcagg caaaaaccat gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg
    13861 cttggggcga acgccagttg accgaatcgg gctatccgcg cgaggtgtgg ctggtggaac
    13921 gcggcaagag tttcgaggaa agcctgcctg tgtatcaaat cgataaaggt gcaatgatgg
    13981 tgaacgcgtg gcgttatctc gatccgcaag gttcgccgat tgatttgatt gaagcgtcgg
    14041 acggttttta caccaaaacc tatttgcagg tgtcgtcgga aggcgaggcg aaaccgttaa
    14101 acctgcccaa cgattgcgac gtggccggct atctagccgg acatcttttg ctgacattgc
    14161 gtaaagactg gcaccgcgcg aaccaaagct atccgagcgg cgcattggtg gcagtaaaat
    14221 taaaccgagg cgaactcggg gcggcgcagc ttttgtttgc gcccgatgaa acgcaggcat
    14281 tggaaagcgt ggaaacgacc aagcgttttg tggtagcgag cctgctggaa aacgtacaag
    14341 gccgtctgaa agcgtggcgg tttgccgacg gcaaatggca ggaagtcgaa ctaccgcgcc
    14401 tgccttcggg cgcgttggaa atgaccgacc aaccgtgggg tggcgacgtg gtttaccttg
    14461 ccgccggcga tttcaccacg ccgctggcgc tgtttgcatt ggatttgaac gtgatggaac
    14521 tgaccgtcat gcgccgtcag ccgcagcagt tcgattcaga cggcatcaac gtgcggcagt
    14581 tttgggcggt ttcgtccgac ggcgagcgca ttccttattt ccatgtcggc aaaaacgccg
    14641 cgcccgacac gccgactttg gtctatgctt acggcggttt cggcgtgccc gagctgccgc
    14701 attatctggg cagcgtcggc aaatactggc tggaagaggg caatgccttc gtgttggcga
    14761 acatacgcgg cggcggcgag ttcggcccgc gctggcatca ggcggcgcag ggcatcagca
    14821 aacacaaaag cgtcgatgat ttgcttgccg tcgtgcgcga tttgtccgaa cgcggcatca
    14881 gttcgcccga acacatcggt ttgcagggcg gcagcaacgg cggactgatt accgccgccg
    14941 ccttcgtgcg cgaaccgcaa agcatcggcg cgctggtgtg cgaagtgccg ctgaccgata
    15001 tgatccgtta tccgctgctc tccgccggtt caagctggac agacgaatac ggcaatccgc
    15061 aaaaatacga agtctgcaaa cgccggctgg gcgaattgtc gccgtatcac aatctttcag
    15121 acggcatcga ttatccgcct gcgctcatca ccaccagcct cagcgacgac cgcgtccatc
    15181 ccgcccacgc gctcaagttc tacgccaaac tgcgtgaaac ctccgcgcaa tcttggctct
    15241 actctccgga cggcggcggc cataccggca acggcaccca acgcgaatcc gccgacgaac
    15301 tcgcctgcgt gttgctgttt ttgaaagagt ttttgggcta agggcgggga gcggcactgc
    15361 cgccgcgaat gaaaaaaggt cgtctgaaac tgctttttca gacgaccttt tttaatggtt
    15421 gtttcaaatc aaaatatcta tgccgccggc cccatcagca cttcttcgca tccgaaggca
    15481 aaaatccgta atgccgcctg aacgcttcat tgagccgtcc cgcgtggcgg tagccgcaaa
    15541 agtgcatggc ggcttggacg gtgctgccgg attcgatgaa ggcgagcgcg tgttccagcc
    15601 gcaggcggcg caggcatccg gcgacggttt cgccggtttg cgctttgaaa tagcgtttca
    15661 ggtagcattc gttcagtccg acgcggcggg cgatttcggc gatggtcagc ggacgggcga
    15721 attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt
    15781 ggcggcggaa ggtgaagcgc aataatcggg cggagagttc cagcgcggcg gcttcgtcgg
    15841 caagcaggcc gaagccgtcc gattcgaacg ggcgttgcag cagtgggcag gccgccgccg
    15901 tcagtgccgc tgcgttttgc gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg
    15961 aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa
    16021 aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag cagggtttcg gatacgtccg
    16081 ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg gcggtattcc ctgccgccca
    16141 tttgtaaccg gttctgcccc gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt
    16201 ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg
    16261 agtcgaagcg gttgatgcac atttccagat gcaaaccggg ctgttttgcc tgcgcaatga
    16321 gcgcgctgtc ggaacagccg tccaacgccc agccggattt atcggagcgg acataggttt
    16381 ggtactggcg gtagatggcg gcggtgttca tgattggata ggaacgagtt gtctaacaaa
    16441 tgaattaaat aggaattatt accaataatc aagcgcaggg attggttgaa acggaaaagg
    16501 tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg
    16561 ggaattttgc gttttgcggc gtggtttctg caggttgttt gcttaataat aaacattctt
    16621 attcgtatgc aaaggaaccg cacaccgtga aaccgcgttt ttattgggca gcctgcgccg
    16681 tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg
    16741 catctgccgc cacactgacc gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc
    16801 ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac gctgaccgaa ttgggcgtga
    16861 atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt gcagcctgca tttgacaagg
    16921 cggcaacggt ggggacgctg ttcgagcccg attacgaagc cctgcaccgc tacaatcctc
    16981 agcttgtcat taccggcggg ccgggcgcgg aagcatatga acagttggcg aaaaacgcga
    17041 ccaccataga tctgacggtg gacaacggca atatccgcac cagcggcgaa aagcagatgg
    17101 agaccttggc gcggattttc ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg
    17161 acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt
    17221 cggttacggg taacaaggtg tccgccttcg gcacgcagtc gcggttggca agttggatac
    17281 acggcgacat cggcctaccg cctgtagacg aatctttacg caacgagggg cacgggcagc
    17341 ctgtttcctt cgaatacatc aaagagaaaa accccgattg gattttcatc atcgaccgta
    17401 ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt attggataac gcgctggtac
    17461 gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg
    17521 tcgcgggcgg ctcgcggcag ttgattcagg cggcggagca gttgaaggag gcgtttgaaa
    17581 aggcagaacc cgttgcggcg gggaaagagt agggagtcgt ctgaaaacgg agtttccgaa
    17641 ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt
    17701 atgttgaaaa taccgctcaa gcaaacctac ggtttgccgc cctctcccta gccctctccc
    17761 acagggagag gggattgggg tgcaggctgc ctttaaggtt taggcaaatt tttaacttcg
    17821 ttgaagctgc gatttcagaa gctccgtttt agcttcgcag aaactccgct tccttcgaaa
    17881 gctccgtttt cagacgacct tttggagtac cgcaggcaca cgcatcgaac ggctgaatca
    17941 aagttcagac cgatggca
//
LOCUS       DAXIHS010000038        17728 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXIHS010000038 DAXIHS010000000
VERSION     DAXIHS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.038"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..240
                     /locus_tag="ACFOF2_001120"
     CDS             <1..240
                     /locus_tag="ACFOF2_001120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107447.1"
                     /translation="LKTEGGSLSSLPSDGISNAAFAPYAGGSPPSFRCLRRMPVASGK
                     PCAGWLCRLKQYEGMRFRRHPPVAEIPSASCPIIA"
     gene            complement(230..1423)
                     /locus_tag="ACFOF2_001121"
     CDS             complement(230..1423)
                     /locus_tag="ACFOF2_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC7107448.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQIQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1420..2403)
                     /gene="patB"
                     /locus_tag="ACFOF2_001122"
     CDS             complement(1420..2403)
                     /gene="patB"
                     /locus_tag="ACFOF2_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC7107449.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2414..3850)
                     /locus_tag="ACFOF2_001123"
     CDS             complement(2414..3850)
                     /locus_tag="ACFOF2_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC7107450.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANASGWNAPQQADMLLLASFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(3978..4133)
                     /locus_tag="ACFOF2_001124"
                     /pseudo
     CDS             complement(3978..4133)
                     /locus_tag="ACFOF2_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            4479..4691
                     /locus_tag="ACFOF2_001125"
     CDS             4479..4691
                     /locus_tag="ACFOF2_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC7107451.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            4839..6422
                     /locus_tag="ACFOF2_001126"
     CDS             4839..6422
                     /locus_tag="ACFOF2_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC7107452.1"
                     /translation="MSSKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIERMGLENVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDTVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLRDIMQFSSRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLDRRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            6504..7601
                     /locus_tag="ACFOF2_001127"
     CDS             6504..7601
                     /locus_tag="ACFOF2_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC7107453.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANTIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGHNPFFSA"
     gene            7715..8830
                     /locus_tag="ACFOF2_001128"
     CDS             7715..8830
                     /locus_tag="ACFOF2_001128"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC7107454.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            8955..9410
                     /locus_tag="ACFOF2_001129"
     CDS             8955..9410
                     /locus_tag="ACFOF2_001129"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC7107455.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            9481..9957
                     /locus_tag="ACFOF2_001130"
     CDS             9481..9957
                     /locus_tag="ACFOF2_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC7107456.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            9945..10871
                     /locus_tag="ACFOF2_001131"
     CDS             9945..10871
                     /locus_tag="ACFOF2_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC7107457.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLVEFHDKAAKLFPPKAKVVEVRNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            10936..11490
                     /locus_tag="ACFOF2_001132"
     CDS             10936..11490
                     /locus_tag="ACFOF2_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC7107458.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVE"
     gene            complement(11729..12688)
                     /gene="ttcA"
                     /locus_tag="ACFOF2_001133"
     CDS             complement(11729..12688)
                     /gene="ttcA"
                     /locus_tag="ACFOF2_001133"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC7107459.1"
                     /translation="MSKKIKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(12781..13644)
                     /locus_tag="ACFOF2_001134"
     CDS             complement(12781..13644)
                     /locus_tag="ACFOF2_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC7107460.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVGAEL
                     EASAEPLAAQEPSLVFTAECVSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTENAE"
     gene            complement(13679..14041)
                     /locus_tag="ACFOF2_001135"
     CDS             complement(13679..14041)
                     /locus_tag="ACFOF2_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC7107461.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            14116..14595
                     /gene="ybaK"
                     /locus_tag="ACFOF2_001136"
     CDS             14116..14595
                     /gene="ybaK"
                     /locus_tag="ACFOF2_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC7107462.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGNGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            14617..15339
                     /locus_tag="ACFOF2_001137"
     CDS             14617..15339
                     /locus_tag="ACFOF2_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC7107463.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            15341..16216
                     /locus_tag="ACFOF2_001138"
     CDS             15341..16216
                     /locus_tag="ACFOF2_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC7107464.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(16288..17685)
                     /gene="aspA"
                     /locus_tag="ACFOF2_001139"
     CDS             complement(16288..17685)
                     /gene="aspA"
                     /locus_tag="ACFOF2_001139"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC7107465.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         4107 a         4362 c         5073 g         4186 t
ORIGIN      
        1 ctgaaaacgg aaggcggcag tttatcatct ttgccttcag acggcatatc aaacgccgcc
       61 tttgcgccgt atgccggagg ttcgcccccg tccttccgat gcttgaggcg aatgccggtt
      121 gcgtccggca aaccgtgcgc ggggtggcta tgccgtctga agcaatatga aggtatgcgt
      181 ttcagacggc atccccctgt tgcggaaata ccgtccgcct cctgtccgat tattgcctga
      241 ttgcagcggc gcggacgagt tcttcgaggc tgtcggcgag catttccgcc gcgcgccggt
      301 agcctttggc ggagaagtgt acgccgtctt tggcggccca tccttggttg agccagtttt
      361 tcatgctgca tatgccgccc atggcgtttt gccaagacca gaacatggtt tgtccctgcc
      421 gggcgacgcg ccgctgtatc tgttggactt cggtcaggcg gacggggcgt gtgccgcata
      481 cgccgagcgt gtttttcagg gattcgggtg cgccgatgat gaggatgccg gcggcaggca
      541 ggctgtcgcg gatttggcgg acggtatcca gccatttttg ttcggtgtcg gcaatgtcga
      601 tgttgtcgcc gaaggcttcg ttggtgccgt aggaaaggat aaccaaatcg gcgccggttt
      661 gggcaaggtc gttcatacgg tcggcacgcc atttcgacca atgggtcaat tgtgcgccgt
      721 tgatgcccat ggcggaaacg gtaatgccgc cggcgggatt ttcgatgttg atgaagccga
      781 tgtcccacgg catttcggtc tgtatggtca agggcagtgc cgcgcgggta tccagtacct
      841 gccagccgcc gccgttggcg gagacggtgt tgccgttgac ggtcagggtt tgttcggcga
      901 gcaggggttt ggcaaacagg gaaatgcgct gtttgccggt cttgccgtcc gatgcggtca
      961 gggtcatgct gccgccgctg ccggtgtggg cgaggatgcc gccgagcggg aagtctccgc
     1021 tattgttcct gctggtgagg ctttgccagt tgccgttgtg ccggactgcc gccatgcgct
     1081 gccctttgac gttggcgggg taaacccagc ctatgccgcc gtcgccccaa gttttttgca
     1141 ggcgtttgcg caggctgtcg gtaaagaagt cgccggcggt atgcgagtcg ccgatttgca
     1201 ggatgcggaa ggtctcgccg ctgccttgtg cgacggatcg gatttttttc atccaaggcg
     1261 aggcggcggc gttgccgtag tcggtcaaga gcccgtttgt atcggtgtag ggggcggacg
     1321 gggaaacggt aacggtgtcg aggctgacgg aggcgacggg cagggcttct gcctgcgtgg
     1381 cggcgaacag ggcggaaaat gcgatgaggt gtttggggtt catggctgtg tacttgatgg
     1441 ttgcgtactt ggttcaaaaa cgattttttc cattattttt gccgccagca gtttttgtcc
     1501 ttcggcggta aagtgtatgc cgtccttgct gcggtagcgg acgggtttgc cgttgacgtt
     1561 gacggagtcg gtgtagcggt cttccccgcc gctcagggtg tgcgcggtgg gaatcaggat
     1621 gattttgcct tccaggtgtt ccgaaagcag tttgtcgagg tagcgcatct gcccgtcgag
     1681 cttggctttt ttcatatagg ggatgccgag ccagacgact tggacgcggt gcgtgtgtgc
     1741 ggcttcaagg atgcggtcga cgcgtttcag gtattcttgc gcccattcgt cggaagcgaa
     1801 tttgaggtat cgtttgccga cggggaaatc ccacgggtcg ttcgggccga ggaagacggc
     1861 gagcacgctg atttcgggat gttttttcag ggtttcttca atcgttttcg gccagtcgaa
     1921 gaatgagggg taggacagcc ccgtgctttg tttgctgagg ttgacggatt cgatgccgta
     1981 ttgctgtttc aggctttttt gcacgaaggg tgcaacgccc tgcatcagcg agtcgccggc
     2041 aaaaaagact ttgtcgccgc tgcggacggc ggcggcttcg gtgtcttgtt tccattctgt
     2101 ttcaccggtt tgagggacgg cttctgatgc ggcggcttca gacggcatat ctgccgaacc
     2161 gccgtcttga gccgtcggcg gcgtttcgcc ggacaggaag gctttgatgc cgtctgaaag
     2221 ggcgtaggcg ttttcttgca acgccacacc gctccgccac catccgtagg cggcgagcgg
     2281 ttcgagcggg ctgttgcggt ggtaggtctg ctgccagtag gcgttgatgg ggttttggct
     2341 gaaccacacg gcaatcaggg cagacatcaa tatggaggcg aaaagggaaa gaaagttttt
     2401 catgtccaaa cccttaaaaa ttggcataaa taaagccggg tatccccgag ggggcgagga
     2461 cgataatcag cagcaggacg gcggaaacgg ggataaacca cagccacatc gggatttttt
     2521 ccaaaccttt gaccacgccg tcgaaagcgc gttgcaggta agggtagagc agcatcacgg
     2581 atgcaaacga ggcaagcagc agcatgtctg cctgttgcgg cgcattccag ccgctggcat
     2641 tggcaaagag cgcgctgaaa accgcgccgg catcgtcggg gtttgcggta ttgaagacga
     2701 caaagctaag gcagacaaaa tggaaggtaa caaaccacga gagcggcgca aggtatttca
     2761 gacggcatag cgcgtcgcgt ccgaaataac ggtcgcccgt gttgagcagc accagcgccg
     2821 tgccgtgcag cgcgccccaa atgaggaagt tccagccgta gccgtgccat atgccggaga
     2881 gtaccattgc cgccatcagg ttgagctgtg tccgtaaaaa gccttttttg ctgccgccca
     2941 aggggatgta gatgtagtcg cgtatccagg tggaaaggct gatgtgccat ttgtcccaaa
     3001 atgcgcggat gtttaaagca cgaagcggtg cggagaagtt tttgggcagc cggaaaccca
     3061 gcagcatcgc catgccgata accaaatcgg aatatccgga aaagtctaaa aagagttgga
     3121 aggtatagcc gtacacgccg cccaatacgc cccagccgtc gaattgggcg ggattttcaa
     3181 atacgggcga cacccagttt tccgccagca tccccgccag ccaccatttt ttggcaatac
     3241 ccagcagaat cagggaaacg gcgagtgcgg ggcggacggg tgagcgcggt tggcgggtac
     3301 ggatttgcgc caatgcgccc gcctgctcgc cgtccgcgct tttgaatgcg gcggcgcgga
     3361 taatcgggcc agaggtaacg gtgggaaaga aactcaggtg tagtagcagc tcgtgccagc
     3421 tgaaacgcgc agcgtgcggg gcgcggaagc agtaaaccag ataggcgagc gactggaagg
     3481 tgtaatacga aagccccagc ggcatcagga tgtcgattgc gccgcctttt ccggcatatt
     3541 gggcaatcag cgggcggaaa aagtcgaaat atttgaaaaa gcccaagacg gtcagcgaga
     3601 cggcaatgcc gcaccccagc caaaagcggc gcgtattttc gcgatcggaa cggagcagtt
     3661 cgcccaaaag gtacacgcag gaggaataaa ggacgacgat tgccgcaaat acggggccga
     3721 tatggtagag ccagcccata ccggcagcca aaagcagcag gttttggacg gacgggtatt
     3781 tcgccaagcc ccagtaaatc ggcaggaagg cgagaaagaa cagtgcgaac tcgacagaca
     3841 gcagcggcat aaaccatcct tggaacaaaa aataggcgtt aaaacacggc aaagcccgtt
     3901 attttaagca ggggtggata tttcttcaat caaaatgccg tctgaagggc gggagaggct
     3961 tcagacggca tcgcgggtca ggttcggata tggcggttca agttcaggca cagccacagc
     4021 ccggccgccg ccgcaagcat accggcgatg ccgatacggg aaataccgac atattgcgtt
     4081 acccaatgtc ccaacagtgc gccgctgccg ataccgacgt tgtatagtgg attaacaaaa
     4141 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggaaccga ttcacttggt
     4201 acttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacga cgtactggtt
     4261 tttgttaatc cactataaat gcgccgattg ccaaggcgat gatgctcaac cagggttttt
     4321 gcgaagcgtt cattgttgtc gtcctgatgg gaattgccgc cattatgcgc aagcgtccgt
     4381 ctttttccaa accgccgcac cgtttccgcg tttgcccaag ttcacgcccc gacttacaat
     4441 aacgcccgtc agattgcagt tccaacgaaa ggaaaatgat ggaaaccctt atcctcgaca
     4501 tcggcggcat gagctgcggc ggctgcgtca aaagcgttac ccggatattg gaaggcgtaa
     4561 aaggcgtggc aagcgtcgaa gtcagccttg aaaacaaaag tgcgaccgtc ggatacgacc
     4621 ccgcgcaaac cgatgcaggg gcgttgattg aagccgttga agacggcggc tatgatgccg
     4681 cgttgaaata aagcggcaaa aatgccgtct gaagccttcg cgcctcctag atgccgtctg
     4741 aagtacgctc cgcgccttca gatggcattt gatttagttg aacgggtagg atgaattggt
     4801 taaacttgcg ggcatattgt ctttagagtg aacaaaatat gtcgagcaag aagaaaaaag
     4861 ccgcttttgc ccgtgctgca acggagaacg gtgtgccgga cgtgtccaac atcaaaaatt
     4921 catatgatga tttgatgtac gaatccggcg cattctctca aaccgccatc aataatttgg
     4981 aggcgcgcgc ccgcctgatg ggcttgcagc ccgcgcccgc cgccaatgcc aaggtcttgg
     5041 agctgggctg ttcgatgggc ggcaacatca tcacgcaggc gctttattac ccggatgcgg
     5101 aatttgtcgg tatcgacttg tccggcaggc aggttgcgca gggcaacgcc atcattgaaa
     5161 gaatgggctt ggaaaatgtg cgtctggaag aaaaagatat tttgaccatc gatgagtcat
     5221 tcgggaagtt cgactatatc atcgtccacg gtatttggtc gtgggttccc gatacggtta
     5281 aagacaaaat cttttcgatt tgccggaaca acctgacgga acacggcatt gcctatattt
     5341 cgtacaatgt ctaccccggc tggaaacggc aggagcagtt gcgcgatatt atgcagtttt
     5401 ccagcaggga tgtgcttgaa gaacccttgg aagcgcgaac gcggaaaggc ttggacgcgc
     5461 tcaaggcact ggcggaaatt ttggaaaacg acaagggctt gggcggcggc ggcaaacttc
     5521 cggcgattca aaaaatattg aatcataatt tttattacat cgcacacgaa tatatggagg
     5581 cgtttaacga cccgatttac gtcaacggct tcatcgaatg ggctaaccgc cacaggctcg
     5641 catatatcgg ggataccaat ttgcacgtgt cttttgtttc ttggatggcg gagcatacgc
     5701 gggagcggat tctggcattg gcaggggacg attatattgc caaagaattt tacagtgata
     5761 ttttatccga ccgccaattc cgccgttcgc ttttatgccg tgaggaagtc ggggatactg
     5821 tcaggcgtga tgagtcggtt gccgtcgaag tgatagaaag tttgaatttc cgcccggcaa
     5881 gaggggaaac aatcaacttt gatgaaaacg atatcctgct ttcgggcata cgcgatgtga
     5941 tgaaaaccgg agaggcgttt aaaacggaag atgttgcgga aaatctcgac cgccgatttc
     6001 ccggtctgga atttgaccgc atgaaaatca attcccagct gttattgcaa accattctcg
     6061 ggcgtttttc tgtttcatca gacaatgcgg gcaaaccatt ttttgaagac cataaaacct
     6121 atgtgccggc gcgttttaca gattatgccg ccgcctttgt ggaacatggc gcggaagcgt
     6181 ttgtccggcc tgccaaccgt tacaacgaaa gcaccccctc attcggatac gggcatttgt
     6241 acattatgcg gcaattgtcg cggccgacga gcaaacaggc attgattgaa acggttgccg
     6301 aaaacttgaa catcgtcagc accacgcccg acggtttgac attccatccg cctgccgaag
     6361 tgtatgtgga agaaatattg gcagacttgg cagacaggca ttttctcgtt tcggcggatt
     6421 gacgggcaag tcccggcggc aatccatccg atatattttc agacaggaaa gacacacact
     6481 acgaaaagcg gtgcacgaca catatgaacc agacatttac cttgcccgat acgcgcccgt
     6541 atccccaaaa tccgattaaa aaccacctgc tgctcaatgc ctaccagttg gcgcataatt
     6601 cttcccaggc ttcgcgcaaa ctctcgtccg gccaacttca aaccgaaatc agggggatgc
     6661 ttgagcaaaa ccactatatc aacctttccc tcgcgctgac gatgtcgccc gatgccggaa
     6721 cttatgccgc gctgctttcc agtgtgaacg cagtactcga ttgcgagaaa gaaggcgaag
     6781 tgcagtggtt cgccctgccg gtcgtgctgg tgtccggctg caagaaagaa cgggcaatcg
     6841 agatgaagct gccgacggag gcattgtttg cctgcctgca aaactatccg cacctgcgcg
     6901 cgttgacgca agagacacaa tggctgcctt atcttgtgca ttcgtccgat ttgagtgcgg
     6961 tcgcgccgga tgagtggtgg cgtgccaaac aaaataccga agcggcggcg caacacttac
     7021 gccgtttcgc cccgcgccct ttgctgttgc ccgaagggca gtccgtccac gtcgtttacg
     7081 cgctgggttt cggcagcggc aaggttcaga cggcattggg tcagaacctt cttcaggcag
     7141 gcctgccctt aatgcaggta tggcaggaaa atcttgcatc ggaaggtgtt acgctgtttg
     7201 ccaatccgct ttccccggat tctccggtac gcgcgctttc agacggcagc cacacgcgcc
     7261 agcgtatggc gatggatgtt tttgcggcaa acacgatacg cgccgtccgt atgcagagtc
     7321 cgcgcgtcgg cgtggtcgct gcggcaaagg cgggcggaca ggttttattc gggtttaatg
     7381 cgaccgacgg cgcgttcgaa gtcgtgccgc aggtgttttc gtggcagctt tcctttaccg
     7441 acaatattgg cgttattcag caaaattttt tggatttaat ggcagaatgc cgtgtagaac
     7501 acgtttacct gttgcacaat cctttgggcg aacaggaaaa catcccgagc tatgcggaag
     7561 cgttgaaacg ggaagggcac aatccgtttt tcagtgcata gtgatttgta gtagtgatag
     7621 gctttctcat ttctataata aatcaaaata aagagcacaa cactttttca ttctgtgttg
     7681 tgccttgagt gaaacgaaag gatgaattat aaatatgaag attgtaattg caccggattc
     7741 gtttaaggaa agcttgacag ctcaacaggt agctgaagca ataaaaagag gcttccaaca
     7801 atcgatagca gatgtggaat gtctcctctg tcctgtcggt gatgggggag aaggcactgt
     7861 agatgctatc cgacattctc ttgacctaga agaaaaatgt ctccaagtga caggaccttt
     7921 tggacaaaaa gaagtcatgc gctattttca aaaagaacaa ctagccctat tcgaagttgc
     7981 tgatttggtt ggtcttggaa aaatcccgct agagaaacga aatccattac aaatccaaac
     8041 tcgtggtatt ggagaattga ttcgccacct cattagtcaa gaaattaaag aaatctatat
     8101 tggcgttggc ggtacggcca gtaatgatgg aggtattggg attgctgctg gtttaggtta
     8161 tcaattttat gatgaggatg gaaatacctt gcccgcttgc ggtcaatcct tactaaacct
     8221 agcttctgtt tcaacagaaa atcgctatga aattcctgaa gatgttcaca ttcgtatttt
     8281 agcagatgtt gtgagtccct tatgtggaca ccaaggtgcg acctatacgt ttggcaagca
     8341 aaaagggttg gattctacta tgtttgaggc cgtagatcag gcaatacaag atttttatga
     8401 aaaagtctcc cctgcaacat taaaacttaa aggagcagga gctggtggag gcatcgctgg
     8461 tggtttgtgc gcctttgctc aggcaagtat cgtatctgga attgacacct gcctggactt
     8521 gattgacttt gataagaaag tgtcagatgt tgacttggtt atcattggtg aaggaagact
     8581 agatcgtcaa agtttagcag ggaaagcgcc tattggtgta gcaaaaagaa cccctgtcgg
     8641 agttcctgtt gtcgctattt gtggcagtct cgttgaagat ttgccttccc tgccatttga
     8701 aaatatccaa gctgcctttt ctattttgga gaaaagtgaa cctttagaag atagtttgaa
     8761 aaatgccagt ctctatttgg agcatacggc ttctaatatt ggacacttat taaatatgcc
     8821 taagatttag ccaaaccatt tcttccagat ggatgttttg gctggttctg ccttatgcgg
     8881 ttacgattaa ccgaccgatg acgtctgaaa gggtttccgc ttcagacggc atcgcgccgg
     8941 aacagggagg ggatatgaaa aaaccgaaaa tcctttttgt ctgcctcggc aacatctgcc
     9001 gttcgccgat ggcggaatac attttgcgcc gccgcgccgc cgaagcgggc attccccttg
     9061 aagcggacag cgcggggaca tcgggctggc acgacggcga agatatgcac cgcgagacgg
     9121 caaagatatt gaaaaaacac ggtatcgatg cttcaggctt taccagccgc aaaatccgcc
     9181 aaagcgatgc ggcggcgttt gactgcatca tcgcgatgga cggcaagaat ttgtccgaat
     9241 tggaaagaac cttcggcagg cggtcggaaa aaatattcaa gctgaccgac ctgatacccg
     9301 aaagcggtta cgaccatgtc cccgatccgt ggtacacggg cgattttgaa gaaaccttca
     9361 ggcttgcgga tgcgggctgt cgggcattgt tggaaaagat ttccaaataa agcaattgaa
     9421 tacaaatata aaaccgcctc ctgccgtatc ggttgttcag acggcataaa cagagaattt
     9481 atgaaaacaa atttcaaaca gaaaattatt gaacaggcac gcagcgaagg cttgcaggta
     9541 accgctttgc gcgagcaggt attagatatt gtcttgcagc aaagcggcgt gattaaagcc
     9601 tacaacgtct tgtcgcagat gcagcagcaa agcgagggcg tgcttgcacc gcctaccgcc
     9661 taccgcgccc ttgatttttg ggcggagcag ggcgttttgc acaaagtggc ggcggtcaac
     9721 ggctatattt tgtgcagcca cgcgcagcac gagtgcgacg accattgcca cgaccacgaa
     9781 gaagccgaag cgcaccacag cgcgtttatt ttggtctgca ccgaatgtgg cacggcggac
     9841 gagcaaaccc tcagccacga gtgggcggca ctgcgcgcag gcgttgccga aagcggcttt
     9901 gcgctgaaag aagaacacgt tgttttaacc gggatttgta aaaaatgtca gaaatgaaaa
     9961 aaaccaaagt ccacctgatt tcaggttttc tgggaacagg caaaaccacc gcgctcaaaa
    10021 gtctgatgga acaaaaagac cccaacgaaa aatgggtcat catcgtcaac gagtttggcg
    10081 aaatcggcat tgacggcgcg gtattgagcg acaacggcat ccctgtggca gaaatcgccg
    10141 gcggctgttt gtgttgcacc gccggcccgc aaatgggcgt aaccgtgcag aaaatgctgc
    10201 gcgatgccaa gcccgaccgc ctgatgattg aggcaagcgg actggcgcac gccgccagcg
    10261 tcatcgacga actcaaagcc aaaccgctgg acagcctttt ggaaatcggc gcggtcttta
    10321 ccgtcgtcga tccgcgccaa ttcatcaacc ccgattacgc gcaacaggca ttgtataaag
    10381 accaaatcgg catttgcgac gtattggccg ccagcaaaac cgatttatgc acccccgaac
    10441 agctggtcga atttcacgac aaagcggcaa aactgttccc gcccaaagcc aaagtggtcg
    10501 aagtccgaaa cgcgcaactc gatatccaat ggctcgatat tcccgtcatc gaaaaatcac
    10561 gctaccgcct caaagccctg ccggacaaca cgatgggctt ccagtcgcaa ggtttcacat
    10621 tccccgccgg acgcgatttc gacggcgaaa aattgaccaa cttcttcaat gatttgccca
    10681 agatgaccga aggactcgtc cgcgccaaag gcgtgtttca agtactcggc acttgggtgt
    10741 ggctcaactg ggtggacggg caatggggtg cgaaccaagt ctcgtggcgg cgcgattcgc
    10801 gttttgaatt gattgccaaa tcgtttgatg cggatttaat cgaacaaaag ctcaaagacg
    10861 cattggaata gcgttcacgc gccttcagac ggcatatgaa atgaaaatgc cgtctgaagg
    10921 tctgaggagg acgggatgaa acccaaattc aaaaccgttt taaccgcgct gcttttggcg
    10981 gtttccctgc cgtctatggc ggcaacccat gttttgatgg aaaccgatat gggcaatatc
    11041 cgtttggttt tggacgaatc caaagcaccc aaaaccgttg ccaatttcgt gcgctatgcc
    11101 cgaaaaggct tttacgacaa tacgattttt caccgcgtca tcggcggctt cgttatccaa
    11161 ggcggcggat tgaccgagga cttggcacaa aaggcaagcg ataaggccgt tgccaacgaa
    11221 tccggcaacg gcttgaaaaa cactgtcggc accatcgcca tggcgcggac gacagacccc
    11281 gattccgcca ccagccaatt ctttatcaat ctggcggaca atgattcact caactacaaa
    11341 aacggacaat acggctatac cgttttcggc agggtcgaaa gcggaatgga caccgtttcc
    11401 aaaatcgccc gcgtcaaaac cgccacgcgc ggcttttatc aaaacgtacc cgtacagccc
    11461 gtcaaaatcc gtcgcgttgt tgtcgagtaa tataacggga aaccttcaga aagctgaaac
    11521 ggaaaaagca ggtgtttggg gttgaaaagc gtctgcttcg ggattttata atggattaaa
    11581 tttaaaccag tacggtgttg tctcgccttg ccgtactatt tgtactgtct gcggcttcgt
    11641 cgccttgtcc taatttaaat ttaatccact ataaaatgcc gtctgaaacg ccaaacgggc
    11701 ttcagacggc atttttgacg gcggggggtt acgcgccgca ggttttcggc ttgttcgcca
    11761 gaatgttgat gactttgcgt tcggcttttt gcggctcgat tttgatttcg ctttcgtctt
    11821 cttcgctgcc gtctgaaaaa cgttcgggca ttttttcgct gtcaaacgcc aaatcgccgc
    11881 cgtgtttcag gctttgaccg cgttccaatc cgacaaagtc gaagagttcg gtatcggcaa
    11941 ggtgggaagg gacgacgttt tgcagggcgg agaacatcga ttcgatgcgg ccggggaagc
    12001 gtttgtccca atcgcgcagc atatcgccga tgacttggcg ttgcaggttg ggttgcgagc
    12061 cgcagaggtt gcacgggatg atggggaatt gttttaattc ggcgtatttg attaagtctt
    12121 tttcttttac atacgccagc ggacggatga cgatgtgttc gccgttgtcg ctcaccagct
    12181 tgggcggcat ggctttgagt ttgccgccgt aaaacatatt taaaaacaag gtggcgagga
    12241 tgtcgtcgcg gtggtgtccc aaggcgattt tggtgcagcc caattctttt gcagtgcggt
    12301 agaggatgcc gcggcgcagg cggctgcaca gcgaacaagt cgttttgcct tcgtctaata
    12361 cgcgtttgac ggtggagtag gtgtcttctt caacgatttt gtagggaacg ccgatgcttt
    12421 cgagataggt cggcaatact tcttcgggga agcccggctg cttttggtcg agattgacgg
    12481 caactagttg gaaatcaatc ggcgcgctgg cttggagccg gcgcaggatg tccaagaggg
    12541 catagctgtc ttttccgccg gagaggcaga ccatgatttt gtcgcccggc tcgatcatat
    12601 tgaaatcgtt aatcgcgtcg ccgacggcgt ggcgcaggcg tttgctgagt ttgttgtttt
    12661 cgagttcttg cttgattttt ttggacataa cggttcgggt ttgaaaattg gaaaggcggc
    12721 attgtaaccg attggcgggg cggcaatgcc gtctgaaggg cttcagacgg catcggcggc
    12781 ttattctgca ttttcggttt taaagaagag atgaaccgct ttgaagatac cgccgtttgg
    12841 gacggtcagt tctttttgtg cggcggagaa tttaatcacg gtaagggcgg taaagttttc
    12901 cgaatcttga acgctgtcga gtacgatgcc ggcctcttcg ccgtccgccg tcagcagggt
    12961 tcctgcttcg acggccgaat ttcccgacaa taccgccaag ccgcgtttga cctgcccccg
    13021 atactgggca cgggcaatga tttcctgtcc cggatagcag ccttttttga agtgtacgcc
    13081 gccgatgatg tgctggttga gcatttgggc gacggcggtt tctttggtag ccgcgcatat
    13141 ccacgggtaa ccgctgcgga tttcgtgcag ccgccacgcg ttttcggcgg cggcatcata
    13201 aggcggcagg gcggtttcgg gggcgatgtg caaaatgccc cgatggggca ggacgacgga
    13261 acagatgccg tctgaaacgc attcggcggt aaagacgaga ctgggttctt gcgcggcaag
    13321 cggttcggcg gatgcttcta attccgcgcc gacggcgtaa tcttcaagga tttcaaaaac
    13381 gactttggca cgcaacacaa acatccgcag gcgtttgacg gttgcttcaa gcaggtcttg
    13441 cgccataatc agcagcaaat cgccgcctcg gttgacgaca atcatattgg cgatgacgcg
    13501 gcctttgggc gtgttgtaag tcgcataaca cgcctgcccg gtctgaagat tattgatgtc
    13561 gttggaaagc tgtccgtgca ggaaggtttg gcggtcttcg ccactgacgc gcaccacgcc
    13621 gaaaaagggc agtaaggttt tcatcatttg cctactctga aaatataagg aaatctgttt
    13681 atgcagttgc cgcgtctctc ttcacggcgg ttattttgat ttcgacgcgc cactccggac
    13741 gggcgagccg cgcttccacg caggcgcggg cgggcgttct gccggcggca acccaagcgt
    13801 cccacacgcc gttcatttcc gcatagtcgc ccatatcgcg cagatagatg accgcatcca
    13861 aaacgtgcgc tttgtccgag ccgcattccg ccagccagcg gtcgatttgg gcaagcacgt
    13921 cggcagtctg ttcggcagcc gtttcaccgt tttcgggaac catgccagag aggaaaatca
    13981 agccgtttgc gccgacggct tcggaatagc ggggcgttgt gccgaaatat cggatatcca
    14041 tatcggtttc cttcgataaa ggggatatat ggtaacattg cgcttgaccg atttccatgt
    14101 tttgcatgac gaaaaatgag taaacacact tatccgataa cacctgccgt gcgcgttttg
    14161 cgtgaaaacg gcatcgaatt tgaacctttt acctatgcct atgaggaaca cggcggcacg
    14221 gcgcagtttg cccgactatt cggcaaagac gaacacttgg tcattaaaac cattgttttg
    14281 caagatgaaa acgggaatgg gctgattgtc ttgatgcacg gcgacaaaca gatttccacg
    14341 cgcaatctgg ctcggcattt gggtgcgaaa cgcatcgaac ccgctacgcc cgcacaggca
    14401 aacaagtgga cgggctatct ggtcggcggc acaacgccgt tcggcatccg gacaaaattg
    14461 gatatttacg tcgaacagtc ggtgatggat ttggaaacca tctatatcaa cggcgggaaa
    14521 cgcgggttca ttatcggtat ccgtcccgga gatttaaata ttttgaaccc gaaaacaata
    14581 caggcggcgg tttgaccggg aagtataagg aacaatatgg acaaagattt gtatgccgta
    14641 ttgggcgtgt cgccgcaggc gggggcggac gaaatcaaac gcgcctaccg caagctggca
    14701 atgaaatacc atcccgaccg caatccgggc aatccgcagg cggaagaaaa gttcaaagaa
    14761 atccaacggg cttacgatac gctttccgac ctgtcgaaac ggatgcaata cgacgcgtcc
    14821 ttcagacggc atgaggaacg cgggcggcag gaagaggcat tccgccgcga acaggcgcgc
    14881 agggagcagt tttaccgcga acagatgcgc cgcgaacagg cgttcagaca ggcgtttgaa
    14941 cggcaggcat cacgttcgtg ccatacttac gaaccgtccg gcggcggaag cgggcgcaac
    15001 tatgtcctcg ccgcctacat cctgttcggt ttgggtgcaa tcatgctgtt catgcccata
    15061 gtcggcgtga ttttcgccta tatgcccata gtcggcgtga ttctcgccta tatgaaacgg
    15121 aacagtttgg acagcattgt ctatgccgca cataccgaat acctgattaa aaccttttgg
    15181 cgcacatttt ggctttatat tttgggtgcg ctgactgccc ttttgggtat cggcgtgctg
    15241 attattattg caacgaacgt ctggtatttc taccgcatca tcgccggctt tatccgcttc
    15301 aacggcggca gggcggttgc acccgagaaa tggatatagt atggcttacc tgttaatcag
    15361 catcgtgttc agcgtgtcgg tttccatttt gctgaaaatg gcaaggaaga aaaaaatcga
    15421 catcgcgcag gcggtcgccg tcaattatgt ggtcgcggtc atactgaccc tgctggtatt
    15481 gaagccggat gtcggcaata tcggcgcatt tttgccgaca tggtggctgt ttgccgcatt
    15541 gggcgtgctg ctgccgtccg tatttgtcat tatgggcaaa tccgtagaat ccgccggtat
    15601 cgtcaaatcc gacgcggcgc agcgtttgtc gctgtttttg ccgattgttg ccgccttgac
    15661 gctgtttggc gaaaaactca gcgaaggcag actaatcggg ctgtgcctcg catttgccgc
    15721 actgttctgc ctgctttgga aacacagcgg tggcaaaaaa tcaggaagcg cgtggcggca
    15781 ggcggcattg ctgctgggcg tgtgggcagg ttacggcatt atcgatatcc tgttcaaaca
    15841 gcttgccaaa agcggaacgg catttgcggg caacctgctg gttgcatttg tgctggcggg
    15901 tgtgctgatg tttgcctgcc tgtttgccaa atcggtcaga tggcgtgttg agagtgtggt
    15961 cggcggcata ttcttgggcg gtttgaattt tatgaatatc gtaacctaca tcaccgcgca
    16021 ccaaatgatg aaggataatc cgaccttggt ttttgccggt atgaatatcg gcgtgattgt
    16081 tttgggtacg ctttcgggcg cattgttctt taaggaaaaa atcaacacaa tcaatacggc
    16141 gggaatcgtg ttggcactgt gttctatcgc ctgcctgttt tattgggggg aagtcaaggt
    16201 actgttcggc atataatccg gctgattgat ataacaaaat gccgtctgaa gcagcatccc
    16261 tgcttcagac ggcatttgtc tgcaacgtta cagatggggg ttcatcaggt tctcgggaga
    16321 gaggatgcgg ttgagttctt cttcgctcaa cagcccgcgt tccaagacaa cctcgcgcac
    16381 gcctttgccg gtttgggcgc agattttgcc gaccaaatcg ccgttgtggt gtccgatata
    16441 cggattcaga taagtcacca aaccgatgga gttgaaaacg taacgttcgc agatttcgcg
    16501 gttgaccgta atgcctttga cgcatttgtc ggacaggttg actgcggcat tgcccaagag
    16561 ggaaatggtt tcaaacatac attgggcgat gaccggctcc atcacgttta attgcagttg
    16621 cccggcttcg gcggcgaagg taatcgtcgt gtcgttgccg atgactttga agcagacttg
    16681 gttgaccact tcgggaatca cgggattgac tttggcgggc attatggaag aaccggcctg
    16741 caattcgggc aggttgattt ctttcaagcc ggcacgcggg ccggaagaga gcaggcgcaa
    16801 gtcgttgcag attttggaga gtttgaccgc cgtgcgcttc aatgcgccgt gtaccatcac
    16861 atatgcgccg cagtcggagg tcgcctcaat caggttttcg gtcagtttgc aaggcaagcc
    16921 gctgacttcg gagagttttt tgaccaccag ttcggcgtag cctttgggcg tgttcacgcc
    16981 cgtgccgatt gccgttgcgc ccaaattgac ttccaacagc agttggcggg tgcggtcgag
    17041 gttgaggatt tcttcttcca acaacacttg gaaagattgg aattcttggc cggcagtcat
    17101 cggcacggca tcttgaagct gggtgcggcc cattttcaaa acgtctttaa actcttcggc
    17161 tttggcggca aaggcgtttt tcaggacggc cagtttgtcg agcaattcgc cgatgctgta
    17221 atacacggca aggcggaagc cggtagggta ggcatcgttg gtggattggc tggcgttgac
    17281 atgatccatc ggattgacga tatcgtagcg gcctttttcg tatcccaaga cttccaatgc
    17341 gaggttggcg atgacttcgt tggtgttcat attgaccgaa gtacccgcac cgccctgata
    17401 cacatcggac gggaattggt cgaggcagcg gttgttcagc agaacttcgt cgcaagcctt
    17461 ttcaatggcg gcggcgattt cgggcttgac cgcgcccaac ttaccgtttg cctgtgccgt
    17521 cgcctttttc accatcacca tactgcggac aaactgcggc acgtcagaaa ttttttgtgt
    17581 ggagatttta aagttttcaa tggcgcgcag ggtgtggatg ccccaataca cttcggcggg
    17641 aatctcgcgg tcgcccaata aatcgtgttc gatacggaca gtcatgtttt tacctttgta
    17701 agtcggataa ttaatattga aaaaatgc
//
LOCUS       DAXIHS010000039        17716 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXIHS010000039 DAXIHS010000000
VERSION     DAXIHS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.039"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(491..922)
                     /locus_tag="ACFOF2_001140"
     CDS             complement(491..922)
                     /locus_tag="ACFOF2_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107466.1"
                     /translation="MMFFQIACNSGNSYISLLKSMRFYIDNKECDYIFFRKAVNISDD
                     FIQSGFITPEGLITKNSNPKLFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLLNDDA
                     YIEFAYSEDFYVLPEIKIDKRTLKSLNFYIDFGVKYIINKI"
     gene            complement(996..1439)
                     /locus_tag="ACFOF2_001141"
     CDS             complement(996..1439)
                     /locus_tag="ACFOF2_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107467.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1482..2351)
                     /locus_tag="ACFOF2_001142"
     CDS             complement(1482..2351)
                     /locus_tag="ACFOF2_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC7107468.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLINPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2515..3438
                     /locus_tag="ACFOF2_001143"
     CDS             2515..3438
                     /locus_tag="ACFOF2_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC7107469.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALALTRTDLPYPLDVVSRLVRGEKFFTTQEVEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3516..4157)
                     /locus_tag="ACFOF2_001144"
     CDS             complement(3516..4157)
                     /locus_tag="ACFOF2_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC7107470.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVSARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGHGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4212..5003)
                     /locus_tag="ACFOF2_001145"
     CDS             complement(4212..5003)
                     /locus_tag="ACFOF2_001145"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC7107471.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFESEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFYL"
     gene            5169..5244
                     /locus_tag="ACFOF2_001146"
     tRNA            5169..5244
                     /locus_tag="ACFOF2_001146"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5202..5204,aa:Thr,seq:tgt)
     gene            5386..6177
                     /gene="panB"
                     /locus_tag="ACFOF2_001147"
     CDS             5386..6177
                     /gene="panB"
                     /locus_tag="ACFOF2_001147"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC7107472.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6167..6343
                     /locus_tag="ACFOF2_001148"
     CDS             6167..6343
                     /locus_tag="ACFOF2_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107473.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6300..7136
                     /gene="panC"
                     /locus_tag="ACFOF2_001149"
     CDS             6300..7136
                     /gene="panC"
                     /locus_tag="ACFOF2_001149"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC7107474.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7301..9139
                     /locus_tag="ACFOF2_001150"
     CDS             7301..9139
                     /locus_tag="ACFOF2_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107475.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPVGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9279..9860
                     /locus_tag="ACFOF2_001151"
     CDS             9279..9860
                     /locus_tag="ACFOF2_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC7107476.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9870..10715
                     /gene="ispE"
                     /locus_tag="ACFOF2_001152"
     CDS             9870..10715
                     /gene="ispE"
                     /locus_tag="ACFOF2_001152"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC7107477.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            10723..10798
                     /locus_tag="ACFOF2_001153"
     tRNA            10723..10798
                     /locus_tag="ACFOF2_001153"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10756..10758,aa:Gln,seq:ttg)
     gene            10807..10882
                     /locus_tag="ACFOF2_001154"
     tRNA            10807..10882
                     /locus_tag="ACFOF2_001154"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10840..10842,aa:Gln,seq:ttg)
     gene            11050..12033
                     /locus_tag="ACFOF2_001155"
     CDS             11050..12033
                     /locus_tag="ACFOF2_001155"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC7107478.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12100..12672
                     /locus_tag="ACFOF2_001156"
     CDS             12100..12672
                     /locus_tag="ACFOF2_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC7107479.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12797..13966)
                     /locus_tag="ACFOF2_001157"
     CDS             complement(12797..13966)
                     /locus_tag="ACFOF2_001157"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC7107480.1"
                     /translation="MTAHKILPVLLSIILGISHATAASPVPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALENVEKRSRWQRLWMRLTGQ"
     gene            14114..15640
                     /gene="ilvA"
                     /locus_tag="ACFOF2_001158"
     CDS             14114..15640
                     /gene="ilvA"
                     /locus_tag="ACFOF2_001158"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC7107481.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15697..16770)
                     /locus_tag="ACFOF2_001159"
     CDS             complement(15697..16770)
                     /locus_tag="ACFOF2_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC7107482.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(16767..17627)
                     /gene="cysW"
                     /locus_tag="ACFOF2_001160"
     CDS             complement(16767..17627)
                     /gene="cysW"
                     /locus_tag="ACFOF2_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC7107483.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAATERNAV"
BASE COUNT         4179 a         4040 c         4940 g         4557 t
ORIGIN      
        1 caagtttatc ctagttatct aggacagccc ctaaactttt tggaagcatc tctaatttat
       61 taatatcgtt catatcccct acacacagaa aaaatcttgg ggaatttcct ttaatctttt
      121 taataaatct tcacaaagcc taaaatgatt taattttagt ttctttttat ttttttgata
      181 aattctagat aacctcgctt attaggaaaa atttcaggat atattccttt ttcttctaaa
      241 gaagtatagt ggattaaatt taaatcagga caaggcgagg aagccgcaga cagtacaaat
      301 agtacggcaa ggcgaggcaa cgccgtacta atccactata tatatccatt catatagaga
      361 aaaattctta tacccaatat aaaacaaggt ctttcattat attttgaaaa acctagaagc
      421 tttgtatcta ttggtttatc aaaattaata attattgcag aagacatgta ggctaataat
      481 cttgatttat ctatatttta ttaattatat atttaacccc gaagtcaata tagaaattta
      541 gtgattttaa agttctctta tcaattttaa tctcagggag tacataaaaa tcctcagaat
      601 aggcaaactc tatatatgca tcatcattca ataataattc aataatattt ttcatttcat
      661 ccgaaagaaa tgtaaccatc tcatattgac atttattttt agataccatt tttgaatatt
      721 gattaaataa ttttggatta ctatttttag taattaatcc ttccggagta ataaaaccac
      781 tttgtataaa gtcatcactt atattaacag ccttccgaaa aaaaatataa tcacactctt
      841 tgttatcaat ataaaatctc atactcttta ataatgaaat ataagaattt ccagaattac
      901 aagcaatttg aaaaaacatc attactccta aaactcataa atagtaagga atatatatat
      961 atgcatattc cttactattt tttcacaaat tatgcttatc tatgcttatt tccataaaaa
     1021 gcatgccctc gaattttttg acttgagttc tggaaatgag aataatttga atggatttcc
     1081 ttctgtccat gtctaataac attttgtctt ccccaagctt ggtttgctac cgctcttgct
     1141 tgttgagatg ttcgtgcata gacattagct cctcttgata aagcttgtgc tgcaacacgc
     1201 tgcgtaaccc aacgctgcgc aaggaatctg cccgcatgca aaattcctat tgccaccaac
     1261 ggtgccgcag ccccttcagt ttctttcatc tccttttgag aaagctccgc caactgcatc
     1321 ggcgcatctg cctgcgtgtg gaacacttgg ttttcaaatg tctgattgtc caagccgttt
     1381 gccattgcgg gggcaatcat agacagcatc attacggctg cggtgatttg ttttttcata
     1441 ataactcctt tggattacaa ggttggaaaa tcaaaacctg cttaaaatgt atgctgtacg
     1501 ccaaatttca gttcggaact gctttgccct gaaacgttga aacgtgcgga tgcgtttaaa
     1561 gccgtggttt tggtgaaacc gaaacctgtg ccgaaatgag cgtaggtgga tgtgtttctt
     1621 gcggtttctt ttttgccgtc tatgcggtcg ggctgtgtgc ccagccattg gatgcctccg
     1681 gtcaggctga ttctgtcgtt ggcggcaaat gagatgttgg ggttgatcag caggtagttg
     1741 cccgatttgt agcggatgcc gtctgaaagg gttttgctgc cgttgatacg gtaggcggcg
     1801 gtgagggaaa ggacaatcgg atctatggct ttgtaggtgg tggcgccgat gagccaggat
     1861 tttcccgacg aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag aaagctgatt
     1921 agggcggggt ttttgtcgtc tttaaggaaa gtgtggctga tgccgaggga tacgtcggac
     1981 atccgtttgt tgcgggtttt gctgttgccg tcgagtttgc gttcttcgtg ccacagatag
     2041 ctgccgctgc cgtaaatgtc ggtattcccg gtcagtccgt agcgcaaacc gagcgtgcca
     2101 acgagcatat cggtattgct gccgttttct tggatttcgg tcggaatggg gataaacgag
     2161 gttgcgccgg tttggatgta aaccggtgcg gcaagttcgg cgcggttgtt ttcgctattc
     2221 aggtaggtaa gggaagtttc cagtttccat ttgcccttgt cagtcattat gtcttcaatc
     2281 gtcaagggca ggtcggcata agcggataaa ggcaggatgg cgggcaaggc gggcaaaaag
     2341 atgcgcttca tatttctcct attgatatta attcttattt actgataata atgaatatcc
     2401 gataatcttg tctaaataaa tttttaaatc ataaaaaaca atatgtttgt ttttgtcaat
     2461 tatgtttcga tatgccgtct gaaaagtttg tgaaaaacgg ttacaatccg ccgcatgaaa
     2521 aaacgcaata atcctcttcc gctgttgaac ggtgtcaaac ccagttattt ggtgctgccg
     2581 catgaaaagc agttttacgg gctgccgctg ctgcattttc tgtgcatccg ctttcctttt
     2641 gtgggcgcgg acgattggcg caggcggttg aacagcggtt ttgtggtcgg ttcggatggt
     2701 gcggcgttgg acgaacatac tttgttcgag ccgggtaagg tgatgtttta ttatcgggaa
     2761 accagcagtg aggatgagcc gcgtattccg tttgaagaaa agattttgca tattgatgag
     2821 catttgattg tggtggacaa gccgcatttt ctgcccgtca tccccagcgg gcgtttttta
     2881 agggaaacgc tgctcacacg tctgcgcctg cgtcccgaat tgcagcattt gaatgttgaa
     2941 gacattacgc cgctgcaccg cttggacaag gatacggcag gcgtgatgtt gttgtcgcac
     3001 aatcctgcca cgcgcggggc ttatcagacg atgtttcaaa acaaaacggt atggaaaacg
     3061 tatgaggcgc ttgcgctgac aaggacggat ttgccgtatc cgctcgatgt ggtttcgcgt
     3121 ttggtaaggg gtgaaaaatt ttttacaacg caagaggtgg aaggcgtgcc aaacgcacat
     3181 acgacggtcg aactgattga aaacaggggg gaattcagcc tttaccgcct tacgccgcat
     3241 accggcaaga aacaccagtt gcgcgtgcat atgatgggct tgggtatgcc gctgttgaat
     3301 gacgcgctct atcccgtgcc gtctgaagcg ggcagcgagg attatcggaa acctttgaag
     3361 ctattggcaa aaaagattgc gtttgcagat cctttgtcgg gtcgggaaag ggtgttttgc
     3421 agcggatttt gcctataatg agagggaacg caaacccgta tcggcggatg ccaccgatac
     3481 gggttttaca ttatcgatgc cgtctgaaag gggctttatc cgttcaggca tacgggcgcg
     3541 aagcggagga tggtgtcgcg caagacgatg agtttgccgt gaaagaaatg cgtggaaccg
     3601 gcgatggtaa tgacgggcaa atcttgcggt tccgcccatt tcagggcttt cccgatttcg
     3661 acgacttcgt cttccgcacc gtgtatcatc agggttttgg caacgttggg aacggcggac
     3721 ggttccggac ggtcggtata gtggcagact gccgcgccga tgagcagtaa caggtcgggt
     3781 atgcgcgctt gtgcggcaaa tgtggcgaca tagccgccga aggagaagcc ggataaggca
     3841 aattcggggg cttcgggatg ttgggcgcgg gcatagtcga tgacggcgag gcagtcttgc
     3901 gtttcgccgt gtccgtaatc gtgtgtgcct tcgctattgc ctacgccgcg aaggttgggc
     3961 aggtagcagt ggaagccgag tttgcttaag gctttggcgg cagtttggat gactttgttg
     4021 gtgtttgtcc cgccttgtag ggggttggga tgattgatga cggcaacacc gcgtgccgat
     4081 acttgttcgg acgggatatg gatggtttct aaaatgccgg caggaccgga tatgtgtatg
     4141 gtttcgggtt tcagcataat gatttcctct tgattttccg catgttccaa tgcggtcatg
     4201 aagatagttg cttaaaggta gaaatgcttg ccgttgttgg ggttggacgc tttcaggccg
     4261 gcaagcatgc ggtggaagtc caatccgtat gcgttgctca gtttgtcggc acgttttttg
     4321 agtttgtcta tgttttgctc tttggggctg ctttggaagg ccattaccgc gacattgccg
     4381 tggctttcgg caggaagttc caatacgcgc ccttcaaaaa cgctcaacaa ccgttcgatg
     4441 aagcgttgat agcgtttgtc gccactccac cagttggtaa cgaatatgcc gtctgaagag
     4501 agtgcgtttc ggcagtctcg gaagaacggt tcttcaacca gcgtatcgat gatttgttcg
     4561 ccgtcgaatc cgtccaccaa gatgatatca gtgttgtggc ggaagacttt gatgtactct
     4621 gcaccatccg cttcaataat ttcaaatttc tcactctcga aaggcaactc aaacaggttg
     4681 cgggcaatgg cgataacctg cgggttgata tctacggcag tttgacgcgt gtcgggcagg
     4741 taggtgtcta tccagcgcgc aaacgagccg ccgcccaagc cgatttgggt gatatgctgc
     4801 ggaagcgcgt cggtaaacag cagccatccc atcatcgcgc ggctgtaaga gagtaccagc
     4861 tcggacgggt ggtcgaggtt cattgagctt tgaacggtgt cgctgcccaa gtgaagcgaa
     4921 cggatattgc cttcttcgga aatgccgact tcgggaaagc cggattttgc aggacgcagg
     4981 cggcggtagg gatgtcttgc catcggtgtg aacggtcggt ttgaaaggcg gatattttat
     5041 cagaatgccg atgcttgtcc tgcttcaaat taatttcttt tcaaataaat tacttattcg
     5101 gatttgccgg ggctttcgga taaattcctt gccaaagtgt gaagttgcct gcataattcg
     5161 ccttctttgc cggtatagct cagttggtag agcacctgac ttgtaatcag ggggtcccga
     5221 gttcgactct tggtgccggc accagatttg acagtccgat tgtgtaaaac agtcgggctg
     5281 ttttgcattt gcggcacggt cggtggcagg gctgcgggca tttcactata atagcccgtt
     5341 tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat atctgatgat tactgtgaac
     5401 acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta tgctgaccgc ttacgaatcc
     5461 agttttgccg cgctgatgga cgatgccggc gtggatgtgc tgctggttgg ggattctttg
     5521 ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca gcctgcgcga tatgtgttat
     5581 cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga ttgtcagcga tttgccgttt
     5641 ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg ccgccgaact gatggctgcc
     5701 ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg cggaaacgac cgaatttctg
     5761 caaatgcgcg gtattccggt ctgtgcccac atcggtctga ccccgcaatc cgtgtttgcc
     5821 ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc aggcgttgct taacgatgcc
     5881 aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg agtgtgttcc tgcggaactg
     5941 gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg gcatcggcgc gggtgtcgat
     6001 tgcgacgggc aggttttggt gatgcacgat atgctcggca ttttcccggg taagacggtg
     6061 aaattcgtca aaaactttat gcaggggcaa agcagcattc aggcggcggt tcgggcgtat
     6121 gttgccgaag tcaaagccaa aaccttccct gctgcggaac atatttttgc agactgaaca
     6181 aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg cattttgttt tgccgtgcgc
     6241 ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg aaggataagg attaccgtta
     6301 tgcaaatcat acataccatt caagaactgc gcgcgtggcg taaaaatgcg ggaacggtgg
     6361 catttgtgcc gaccatgggc aatctgcacg aagggcatct tgccctcgtt cgcgaagcca
     6421 aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa ccgcctgcaa ttcggtcagg
     6481 gcgaggattt cgacaaatat ccgcgtactt tgcaacagga tgcggacaag ctggaagccg
     6541 agggtgttgc cgttgttttc gcgcccgatg agaaagaact ctatccgaac gtggaacagc
     6601 gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg cggcaagttt cgtccgggac
     6661 atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa tattgtcgcg ccggatgttg
     6721 cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat taaaggtttg accgaggatt
     6781 tgaattttga tgttgaaata gtgcctgtcg atacggggcg cgcggaagac ggtttggcac
     6841 tctccagccg caaccagtat ttgagtgcgg cggaacgcga cgaagcaccg cgcctgtacc
     6901 gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag tttggattat gcaggtttgg
     6961 aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt ggtcgattat gtcgaaatcc
     7021 gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa gaaactggtg gtcttggccg
     7081 ccgcccgtct ggggacgacg cgcctgattg acaatttgga aataaaactc ccttaaaccg
     7141 caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca gacggcattt atttttttga
     7201 acggggtttc gcaaatctac aaacgattct gcttgtgata aagttacgcc tgattatatg
     7261 ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt atgttacccg cccgtttcac
     7321 cattttatct gtgctcgcgg cagccctgct tgccgggcag gcgtatgccg ccggcgcggc
     7381 ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga aagcagcagc gttacagcga
     7441 ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg ggcgagcggg ttaatcagat
     7501 atttacgttg ctgggagggg aaaccgcctt gcaaaagggg caggcgggaa cggctctggc
     7561 aacctatatg ctgatgttgg aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga
     7621 aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg atttatcaga aatggcggca
     7681 gattgagcct ataccgggtg aggcgcaaaa acgggcgggg tggctgcgga acgtgctgag
     7741 ggaaagagga aatcagcatc tggacggact ggaagaagtg ctggctcagg cggacgaagg
     7801 acagaaccgc agggtgtttt tattgttggc acaagccgcc gtgcaacagg acgggttggc
     7861 gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa tatgaacatc tgcccgaagc
     7921 ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa aaggaaaagg caatcggagc
     7981 tttgcagcgt ttggcgaagc tcgatacgga aatattgccc cccactttaa tgacgttgcg
     8041 tctgactgca cgcaaatatc ccgaaatact cgacggcttt ttcgagcaga cagacaccca
     8101 aaacctttcg gccgtctggc aggaaatgga aattatgaat ctggtttccc tgcacaggtt
     8161 ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc aatccgaatg cagacctgta
     8221 tattcaggca gcgatattgg cggcaaaccg aaaagaaggt gcttccgtta tcgacggcta
     8281 cgccgaaaag gcatacggca gggggacggg ggaacagcgg ggcagggcgg caatgacggc
     8341 ggcgatgata tatgccgaca gaagggatta caccaaagtc aggcagtggc tgaaaaaagt
     8401 gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg gctgcggcgg ctgtcgagtt
     8461 ggacggcggc agggcggctt tgcggcagat cggcagggtg cggaaacttc ccgaacagca
     8521 ggggcggtat tttacggcag acaatttgtc caaaatacag atgctcgccc tgtcgaagct
     8581 gcccgacaaa cgggaggctt tgagggggtt ggacaagatt atcgaaaaac cgcctgtcgg
     8641 cagcaataca gagttacagg cagaggcatt ggtacagcgg tcagttgttt acgaccggct
     8701 tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg ttcaggcttg cacccgataa
     8761 cgctcagatt atgaataatc tgggctacag cctgctttcc gattccaaac gtttggacga
     8821 aggcttcgcc ctgcttcaga cggcatacca aatcaacccg gacgataccg ctgtcaacga
     8881 cagcataggc tgggcgtatt acctgaaagg cgacgcggaa agcgcgctgc cgtatctgcg
     8941 gtattcgttt gaaaacgacc ccgagcccga agttgccgcc catttgggcg aagtgttgtg
     9001 ggcattgggc gaacgcgatc aggcggttga cgtatggacg caggcggcac accttacggg
     9061 agacaagaaa atatggcggg aaacgctcaa acgtcacggc atcgcattgc cccaaccttc
     9121 ccgcaaaccc cggaaataat gcaggtccat cctttcagac ggcataaggt ttgccgggaa
     9181 gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt tttgcacggc acaccgaacc
     9241 catcggccgg caggatggca tccgttaagg aaattctgat gaaacacact gtatccgcat
     9301 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
     9361 agccgtccga acacacccgc agtttcacgg cagaagggcg gttggcagtg aaagcggaag
     9421 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
     9481 tcaatacccc tttgggcagt acgctcgggc agttgtgtca agacagggac ggcgcattgg
     9541 cagtggacgg caaaggaaat gtctatcagg cggaaagtgc ggaagaattg agcaggcagc
     9601 tggtcggttt caaactgcca atccaatatc tgcatatctg ggcagatggc aggcgtgtgg
     9661 cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga acaatacggt tggactgtcg
     9721 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
     9781 acatcaggct ggttttcacc gaaatcggta tgccgtctga aaccgaaacc ccggaacgct
     9841 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
     9901 ccgcacctgc aaaactgaat ctcgatttga ggattaccgg caggcgggaa gacggttatc
     9961 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaaccga
    10021 gggacgacgg caaaatcatc ctgcacaatc ctgtcggcgg aataccgcag gaagccgatt
    10081 tgagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa
    10141 tatggctgga caaaaaaatc ccgacagggg cgggtttggg cggcggaagc tcggatgcgg
    10201 caaccgtttt gctggtgttg aaccgttggt ggcagtgttc ccgaacgacg cagcagctga
    10261 ttgacttggg cgcaactttg ggtgcggacg taccattttt tattttcgga aaaaatgctt
    10321 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtca
    10381 tcgtcaaacc gcccgtccac gtttccactg caaaaatttt cacacacgaa ggcttgacac
    10441 gaaattccgc ctcaagcata atgccgactt tccaaaacct gcaaccgttt agaaatgata
    10501 tgcaggcagt tgtatttaaa gaataccctg aagtttggaa agcctattcc gaattgtccc
    10561 gatatggatt tgccttaatg acaggttctg gtgcgtgtgt attcacggcg tgtcaagata
    10621 gggatagcgc atacaatata taccgacaag tttcaggttt gtacgaggca tatttggcag
    10681 agggtctttc aaaacatcct ttgttgtccg tataaacatt gttggggagt cgtcaagcgg
    10741 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccagc
    10801 cctttttggg gagtcgtcaa gcggttaaga cactggattt tgattccagc atgcgaaggt
    10861 tcgaatcctt cctccccagc cagataaaag cgtgtaagcc tgcttacacg ctttttattt
    10921 catagaaata aaaatattga aatgcctttg tttgtgtcgg atgttgcagg tataatgtcg
    10981 ggcttggtac aagcagaggg aagcattgtg ttttctgagc ggaagttaaa cataaaatca
    11041 ggtgagaata tggctgcgta cgacagtttg atggtattta caggcaatgc caatcccgaa
    11101 ttggcacaac gtgttgtcag gcatttggac atttctttgg gcaatgcttc cgtatccaag
    11161 ttttcagacg gcgaagttgc cgtcgaactg ttggaaaacg tacgcgggcg cgatgttttc
    11221 atccttcagc cgacctgtgc gccgaccaat gacaacctga tggaaatcct gacgatggcg
    11281 gatgcactga agcgtgcttc ggcaggtcgt attaccacag ccattccgta tttcggctat
    11341 gcgcgccaag accgccgtcc gcgttccgtc cgcgttccga tttctgccaa actggtggca
    11401 aatatgctgt attcggcagg gatcgaccgt gttttgactg tcgatttgca tgccgaccag
    11461 attcaaggtt tcttcgatat tccagtggac aatatttatg ccaccccgat tctgttgaac
    11521 gacatcaaac aacagcggat tgaaaatctg accgtcgtca gcccggacat cggcggtgtc
    11581 gtccgcgccc gcgccgtggc aaaatccctg aatgccgact tggcaatcat cgacaaacgc
    11641 cgcccgaaag ccaatgtggc ggaagtcatg aacatcatcg gcgatattca aggcagaacc
    11701 tgtctgattg tggacgatat gattgacact gcaaatacgc tgtgcaaagc cgccgtcgct
    11761 ctgaaagagc ggggggcgga acgtgtcttg gcatatgcca gccacgccgt attctccgga
    11821 gaggcggtca gccgtatcgc ctcatccgaa atcgaccagg tggtcgtaac cgataccatt
    11881 cctttgtctg aagcggctaa aaactgcgac cgtatccgtc aggtaacgat tgccggtctg
    11941 ttggccgaaa ccgtccgccg catcagcaat gaagaatccg tctcatatct tttcaatgaa
    12001 gaagtgatga caggcagtat gttgctgcca taagcccgaa gccgtcttaa gctggtcgcg
    12061 gccgatgacg gcgattttac ctaacttgga gtatttaaca tgacttatga aattcaagcc
    12121 tctgttcgtg aagcacaagg cactggtgcg agccgccgcc tgcgtcgcga aggccaaatc
    12181 cccggcattc tgtacggtga aggtcaagag cctgttgcaa tcgctgtgga tcacaaaacc
    12241 gtattctacg cattggaaaa agaatctttc cataccgcgt tgattaaatt gtctctgaac
    12301 ggcgaaacca aagacgttat cgtccgtgat ttccaaatgc acccgttccg ccgcgaagtt
    12361 caacacatcg acttccaagc tgtgaaagcc gatcaacttg tacgcatccg tgttcccctg
    12421 cacatcgtta acgctgaaaa ttcccaagcg gtcaaactgc aaggcggccg tgtatctctg
    12481 ttgaacacga ctgttgaagt gcttgcattg cctgccaata tccctgcttt cttggatttg
    12541 gattgtgccg aagtggttgc cggcgacatt ctgcatctgt ccgacatcaa actgcctgaa
    12601 ggtgtagaaa gcgtttcctt gaaacgtaac gaaaatctgg ctgttgccac cgttaccggt
    12661 aaaaaacgct aattgatttc agcagcaggg cggcgcgtat gcaatacgta ccgccctgtt
    12721 gttttatgcc gtctgaaccg tgtttcagac ggcatttctt tatttgttgg aaaaacggga
    12781 tatttgaaac ggcagattac tgtcccgtca gacgcatcca aagcctttgc caccggcttc
    12841 ttttttctac attttccagt gcgacaattt ctttttcggt aatggtgtat ccgttttgcc
    12901 tgattttgat ttttcctaaa atctgtccct tttttaccgg ggcgggaatc ggctgtatgg
    12961 tttctagaat ttgttctgcc attttcgctt ccttatgtgg cagagtgatg taggcttctt
    13021 tgaggaagcc tgcgcggacg gtttttttgc tgcctccgga aatttggatt tgggcaacgg
    13081 ttttgccttt cggatatatt ttgggcgtat cgaaggcctg caatgcccag ttcagcagct
    13141 tgctgttgtc tgatgcgcgt gtttccgccg attccgaacc caatgtgatg acaaggatgt
    13201 gcctgccgtt gccggagtat gacacggcaa ggttgtagcc gccgctttct gtgtgtccgg
    13261 ctttcagacc gtttacattg ttgtccctat ataaaaggat attgcggttg ttttgctcta
    13321 tatttttgaa tttgaaagat ttgatggaaa acagcgggta atattccgga aagtcgcgca
    13381 tcaatgcttc agacagctgg gcgaggtctt tggcggtgga aacctgtcct tctctactca
    13441 agcctgtcgg gtttttgaat acagtgttct tcatgcccaa gcgtcgggct tctttgttca
    13501 tttgttgcac aaaattttca atcgagccgt tgcccagccg gccggcaagg gttagggcgg
    13561 catcgtttgc ggatagggca atcatgcctt ttaagagttt gtcggtgctg accgtatcgc
    13621 cgggacgtac aaacattctg cttccttctg aagcccatgc ggattcgggt atttttaagt
    13681 tttcttcaga ttggatattt cccgatttca tgtttttgaa aaccaaatat gcggtcatca
    13741 gttgggttag tgccgccggt tcgacagggg tattgatgtt tttggcggat aaagtctgtc
    13801 tgctttgaag gtcgataacg atgtgtgccg ctgtgagggt ttcgggtgtt tggagcgtgg
    13861 gggcggcgtg tgccgtcggt ctgttgggca cgggcgatgc agccgttgcg tgagaaatgc
    13921 ctaagatgat ggaaagcagg acgggcagga ttttatgtgc tgtcatgaaa tattctaatt
    13981 gtgtgcgtgt ttcagtctgc cgattatacg cttagggtgt ctgatcgggc ggatttttct
    14041 tgctttcgcg ccgtcttggg cgtatggttt tgggttttgc gattttaata aaccgattat
    14101 cccatattga attatgaaca cgccccttcc ttattccaat tacctcatcc gcatcctgac
    14161 ggcatctgtc tatgatgtcg cggtcgaaac gcctttggaa ccggcacgca gcctttctgt
    14221 acgtttgaaa aacaacatcc ttttgaaacg cgaagatttg cagccggttt tttcgttcaa
    14281 aatacgcggc gcgtacaaca aaatgtccaa gttgccgaaa gatgcgctcg cttgcggcgt
    14341 gattgcggca agcgcgggca atcatgctca aggcgtggca ttgtccgcac agcgtttggg
    14401 ctgccgtgcc gttatcgtta tgccggagac tacgccgaaa atcaaagtgg atgcggttaa
    14461 gagccgtggc ggcgaggtgg ttttgcgggg cgtttcatac aacgatgcct acgattatgc
    14521 gatggagttg gcggaaaaag aagggttaac ctatatcgcg ccgtttgatg atcctgatgt
    14581 gattgcggga caggggacgg tggggatgga aattgtcagc cagcatcccg atccaatccg
    14641 cgccgtattc gtaccgatag gcggtggcgg tttggcggcg ggcgtggcgg catttatcaa
    14701 gcaggtccgt cccgaaatca aagttatcgg cgttcagacc aacgattcct gctgtatgaa
    14761 gcagtcggtc gaagcgggtg aaatcgtcca tttgaaagat gtcgggctgt tttcagacgg
    14821 cactgcggtc aaagtcgtcg gaaacgaaac cttccgcctc tgcaaagaac ttttggatga
    14881 aatcattaca gtcgataccg atgcggtttg cggcgcggtc aaggatattt tcgatgacac
    14941 gcgcagcatt accgagccgg cgggcgcgtt ggcgttggcg ggtctgaaag cctatatcgc
    15001 ccgagaaggc gcggaaaacc aaaccctgat tgccgttacc agcggtgcga atatgaattt
    15061 tcaccgtttg cgccatgtgt cggagcgtag tgaattgggc gagggcaacg aaggtatttt
    15121 tgccgttacc atccccgaag aacgcggcag cttccttaag tttgtcaata tattgggaaa
    15181 taggaatatt accgagttca actaccgcta cggagacgat gaaaaagcgc atatctttgt
    15241 cggacttcaa gcggcaggtc cgcaggattt ggcggttatc ggtagccggt tggatgaggc
    15301 gggattgccc aatgtcgacc tgaccgatga tgagattgcc aaaatccata tccgctatat
    15361 ggtcggaggg cggacggaca aagtagaaaa cgagcgtctg gtcagttttg agtttccgga
    15421 gcgtccgggg gcgttggcgc gttttttgaa ccatatgcag ggcggttgga acattacgct
    15481 cttccattac cgcaaccacg gtgcggatta cgggcggatt ttggtcggta tcgatgtgcc
    15541 gccgcacgat gccgccgcat ttgacggttt cttggaaagt ctgggataca gctatcacga
    15601 ggaaacgcaa aatgccgcgt acaagctgtt tcttgcctga cgcttgaaag cacaatgccg
    15661 tctgaaagcc tttcagacgg cattgcgctt tcatggttaa atcgaatatt caatcagttc
    15721 gttttgagag aagacataaa cctgtttcgg aatcagcttc aattctttgc cttcggcgat
    15781 tgggtaacgc gcggcatcgc tgccagccag cgtgatatgc acgtcttgtt tgccgtgttt
    15841 caccaaaata tgcgtcaatg ccccgacggc gtggactttt tcgatttcgg cacaaatcat
    15901 cggtgtttcg tgttcggcgg cgatctgcca ttcgtgcggg cggatatagc cggtggcggt
    15961 ttgttcctgc catttgtatt gtgcgtccaa tttccacgcg aagccgttat aatgccagaa
    16021 gcctttttcg atacgtcctt caaaagcgtc ggtttcgccg aggaactcgg taacgaaggc
    16081 attttcgggt ttgcggtaaa tagcttcggc gctgccggtt tgttcgattt tgccgtggtt
    16141 catcacgacg atttcgtcgg aaacttcgag ggcttcttct tggtcgtgcg taaccagaat
    16201 gctggttaca cccaggttgt gatggatgtc gcgcagccag gtgcgtaatt ctttgcgtac
    16261 tttggcgtcc aacgcgccga agggttcgtc caaaagcagg agtttcggtt cgaccgcaag
    16321 cgcacgggcg agggcgatgc gctggcgttg cccgccggag agttggtgcg gataggattt
    16381 tgccaaatgc gagagttgta cgagtttcag caattcttcg actttagtgc agatttgttc
    16441 tttggacggg cgttcggact tgggcaatac ggtcaaaccg aaagcgacgt tgtcaaacac
    16501 gttcatgtgg cggaagaggg cgtagtgttg gaacacgaag ccgactttgc gctcgcggac
    16561 gtgtttggcg gttacgtctt gcccatcaaa cagaatcttg ccgccgtcgg cgttttccag
    16621 tccggcgata atgcgtaaaa gcgtggtttt gccgcagccg gacgggccga gcagggaaac
    16681 gagtttgccg gtggggacgt tgaggttgat gtttttcagc gcgtgaaaat tgccgaagtg
    16741 tttgtttaag ttttggatgg tgatactcat actgcgttcc tttcggtggc ggcgagtttt
    16801 ttgtcttgta atttggtgat gatgttttgc accgccagcg tcgccagtgc taagagtgcc
    16861 aatacgccgg agagggcgaa tgcgccggtg aagttgtatt cgttgtagaa gatttcaacc
    16921 aaaagcggga tggtgttggt ctcgccgcgt atgtgtcccg ataccacgct gaccgcgccg
    16981 aattcgccca tcgcgcgggc gttggtaagg atgatgccgt agagtaacgc ccatttgatg
    17041 ttgggcaggg taacgcgcca aaacatctgc cagccgcttg cgccgagtat caatgccgcc
    17101 tgttcttcgc tgtcgccctg tgcctgcatc agcgggatga tttcgcgtgc gacaaaaggg
    17161 aaggtaacga acagcgtcgc caaaataatg ccggggatgg cgaaaataat ctgtatgcct
    17221 tgcgcttcga gccagccacc caatgccgtg tgcgcgccga acaataagac gaacattaaa
    17281 ccggccacca cgggcgatac ggaaaacggc aaatcgagca gggtagtcag caactgcttg
    17341 ccgcgaaaat caaaacgggt cagcagccac gccatcgcca cgcccaagac ggcattgacg
    17401 gggacgacaa tcagcgcggt aatcagcgtc aatttgatgg cagaccacgc ttcgggatcg
    17461 tttaaggatt tcaggtacaa atcccaaccg ccttttaagg cttcgtaaaa cacggcgacg
    17521 agcggcacga ccagcatcag cagcagaaag cccagcgcgg cggcaatcag caacacgcgc
    17581 agccggcgcg gttcggtcag gttgggattg gcggaatagg gtttcatggc ggtggtgttc
    17641 cccggttggt tgttttaagg tgttcggatg ccgtctgaaa aaccgttttt tgtggggcgg
    17701 attcgttttc agacaa
//
LOCUS       DAXIHS010000040        17229 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXIHS010000040 DAXIHS010000000
VERSION     DAXIHS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.040"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..424
                     /locus_tag="ACFOF2_001161"
     CDS             <1..424
                     /locus_tag="ACFOF2_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HFC7107484.1"
                     /translation="EVPAVEENAAETVADDLSALLQPAEAPAVEENATEITLETPDSN
                     TSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKYDLAEM
                     YLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(597..1013)
                     /gene="gloA"
                     /locus_tag="ACFOF2_001162"
     CDS             complement(597..1013)
                     /gene="gloA"
                     /locus_tag="ACFOF2_001162"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC7107485.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(1038..2207)
                     /gene="lapB"
                     /locus_tag="ACFOF2_001163"
     CDS             complement(1038..2207)
                     /gene="lapB"
                     /locus_tag="ACFOF2_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC7107486.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2218..2532)
                     /locus_tag="ACFOF2_001164"
     CDS             complement(2218..2532)
                     /locus_tag="ACFOF2_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC7107487.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2842..3840
                     /gene="ilvE"
                     /locus_tag="ACFOF2_001165"
     CDS             2842..3840
                     /gene="ilvE"
                     /locus_tag="ACFOF2_001165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC7107488.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(4025..4810)
                     /gene="fabI"
                     /locus_tag="ACFOF2_001166"
     CDS             complement(4025..4810)
                     /gene="fabI"
                     /locus_tag="ACFOF2_001166"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC7107489.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4965..5786)
                     /gene="dapD"
                     /locus_tag="ACFOF2_001167"
     CDS             complement(4965..5786)
                     /gene="dapD"
                     /locus_tag="ACFOF2_001167"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC7107490.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5953..7596)
                     /gene="pgi"
                     /locus_tag="ACFOF2_001168"
     CDS             complement(5953..7596)
                     /gene="pgi"
                     /locus_tag="ACFOF2_001168"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC7107491.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8106..9338
                     /gene="pilG"
                     /locus_tag="ACFOF2_001169"
     CDS             8106..9338
                     /gene="pilG"
                     /locus_tag="ACFOF2_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC7107492.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9412..10272
                     /locus_tag="ACFOF2_001170"
     CDS             9412..10272
                     /locus_tag="ACFOF2_001170"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC7107493.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10274..10906
                     /gene="coaE"
                     /locus_tag="ACFOF2_001171"
     CDS             10274..10906
                     /gene="coaE"
                     /locus_tag="ACFOF2_001171"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC7107494.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            10899..11108
                     /locus_tag="ACFOF2_001172"
     CDS             10899..11108
                     /locus_tag="ACFOF2_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC7107495.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11193..12869)
                     /gene="pilB"
                     /locus_tag="ACFOF2_001173"
     CDS             complement(11193..12869)
                     /gene="pilB"
                     /locus_tag="ACFOF2_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC7107496.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(13268..13339)
                     /locus_tag="ACFOF2_001174"
     tRNA            complement(13268..13339)
                     /locus_tag="ACFOF2_001174"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13304..13306),aa:Arg,seq:cct)
     gene            complement(13359..13805)
                     /locus_tag="ACFOF2_001175"
     CDS             complement(13359..13805)
                     /locus_tag="ACFOF2_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC7107497.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(13810..14784)
                     /gene="ispB"
                     /locus_tag="ACFOF2_001176"
     CDS             complement(13810..14784)
                     /gene="ispB"
                     /locus_tag="ACFOF2_001176"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC7107498.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            15008..15316
                     /gene="rplU"
                     /locus_tag="ACFOF2_001177"
     CDS             15008..15316
                     /gene="rplU"
                     /locus_tag="ACFOF2_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC7107499.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            15341..15613
                     /gene="rpmA"
                     /locus_tag="ACFOF2_001178"
     CDS             15341..15613
                     /gene="rpmA"
                     /locus_tag="ACFOF2_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC7107500.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            16001..17185
                     /gene="ubiM"
                     /locus_tag="ACFOF2_001179"
     CDS             16001..17185
                     /gene="ubiM"
                     /locus_tag="ACFOF2_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC7107501.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         4190 a         4767 c         4331 g         3941 t
ORIGIN      
        1 tgaagtacct gccgttgagg aaaatgcagc ggaaaccgtt gccgatgatt tgtccgcact
       61 gttgcaacct gctgaagcac cggccgttga ggaaaatgca acggaaatca ctttggaaac
      121 gcctgattcc aacacctctg aggcagacgc tttgcccgac ttcctgaaag acggcgagga
      181 ggaaacggta gattggagca tctacctctc ggaagaaaat atcccaaata atgcagatac
      241 cggtttccct tcggaatctg tgggttctga cgcgccttcc gaagcgaaat acgaccttgc
      301 cgaaatgtat ctcgaaatcg gcgaccgcga tgccgctgcc gagacagtgc agaaattgct
      361 ggaagaagcg gaaggcgacg tactcaaacg tgcccaagca ttggcgcagg aattgggtat
      421 ttgattccca actgcccttt cgcagatcaa ggatgccgtt tcagacggca tctttttttg
      481 ccttatcggt gtaacggata aagtttgaac cggcacaggc tcaaacagca ggtcgacggc
      541 aacaaaatgc cgtctgaaac ccctaaaggc ttcagacggc attggcggcg gcggtatcag
      601 gcagtttgat aggcaaccga atcgtcgccg cttttctttt gaatgaactc gattttgtat
      661 ccgtcggggt cttcgacgaa ggctatcacg gttgtgccgt gtttcatcgg gccggcttcg
      721 cggacgacgt ttccgccctg ccgcttcaca cgttcgcagg cttcgtaggc atcgtccact
      781 tcaaccgcga tgtgtccgta ggcgttgccc aagtcgtatc gttccgtatc ccagttgtgc
      841 gtcagttcca aaaccgtgct gtcggtttca tcgccgtaac cgacgaaggc aagggtaaat
      901 ctgccttcgg gataatcttt tcggcggagc agtttcatac ccaaaacgtt ttggtagaaa
      961 tcgagggatt tttcgagatt gcccacgcgg agcatagtat ggagtaagcg cattttttgt
     1021 gttcctttcg gtggtggtta aacttcgatt ttattcgggg taaacgtctg ccatttgttg
     1081 caggcggggc agtgccagaa aaagacttgt gatttgaagt ggcagtttcg gcaccggtac
     1141 atcacgctgc gctgtagctg ccgtccgata accgaacgca tcatatcggc atcggctttc
     1201 caagccggat ccaaatcgct gagtttcaaa ccaagcaggc ggtacacgcc gttgaggtcg
     1261 ggcttgcggc ggacaagctc gacggcggtt tgcgcggctt ctttctcgca cttaagcagc
     1321 agggatttct cgtacacgac attgatcagg tcaagttcgg gaaacgtctg catatatcct
     1381 gtcagacggt tcaagccttc ttcaggtttt ccctgcgcgg cataggcttc gtaaagcttc
     1441 tcgccgacca tgctcaagta tgcatggttt tgctgctcga tggcggcata ggcttcgacg
     1501 gcggcaggga aattgccttg tcggtgttcg atgtcgccca aaatcatgtt ggcacgggtg
     1561 cattttttgt tggcttcgag tgccttgccg acattgaaac gcgcgacatc gaaattggac
     1621 ttgaacagcg cggcttgggc aagttcgcaa taaaactggg cgatttcaaa ctgataggtc
     1681 tgatcgtcat ggctgagcag ccgggcggtt tcaaccgctt tttcccaatc cctgtcctgt
     1741 tggtagatat tgagcaggtg ctgtctggct tcacgcgcca ttttaccgtc ttgcagcccc
     1801 aaaaaaatct gttcggcacg atcgaccaac cccgcacttt ggtagttttg cgccaattca
     1861 aacaggacgc gcgcgcgctt ttcgccgacc gtatcgggag aatcgagcat tgtccggtgt
     1921 atgttgatgg ctttgtcgtt ttcgccacgc tgacggtaaa gcttgcctag ggtgaggttt
     1981 aggtcgtatg actgaggctg ctgatcaatg acctctgcca attccctggc ggcacgtccg
     2041 ctgttgcggt caaccaaggc atccagactt ttataaaatc ccgacggtat gctttttgcc
     2101 tgctttaata cagtcttcat atccacacgg gcggcaaacc agcccatcgc gaagaaaacg
     2161 ggcaaaagga taatcggcag caggataatc cacaattcat tgtccatatc ggctttctca
     2221 aggctgtttg gcagattcgg gcgcattttg cgccggtggt gcggtcagct ctttccccgt
     2281 caaacgcgca tttttcttta cttcggcacg caacctgccg ttctcgccac gtaacgacaa
     2341 caaccgtccg aacaaggcaa acattccgaa cacgatgccg acgacaaacg cgccgaacaa
     2401 tacgacaatc agcggcaaat cgactttctg tccgggcagg taggagaagg taacggcatc
     2461 cgtattaatg acggcaagca gcaggaagag cagcaggata atgattttga tgacggtata
     2521 gataagtttc atgtttgctc cgtaaataaa aacgccgccc ggacagcttc gcactctgaa
     2581 ccaagattgc gctagtttaa acgatttgca cggtttcgga taggatgcgg cagcgtgttc
     2641 ggacgacata cggagtatgc gattgccgac aattttacca aatacaccgt tcctttccat
     2701 ttgaaaaata acggattgga caccgcatcg acagaaaaac ccgccgcgca cttgtcaaaa
     2761 ccctgtttgc aggcgtatct ttacaatctt caaattcaaa ccgttcattg aaacatatca
     2821 gaataagaaa ggctttacat catgagcaga cccgtacccg ccgtattcgg cagcgttttt
     2881 cacagtcaaa tgcccgtcct cgcctaccgc gaaggcaaat ggcagccgac cgaatggcaa
     2941 tcttcccaag acctctccct cgcaccgggt gcgcacgccc tgcactacag cagcgaatgt
     3001 ttcgagggac tgaaagcctt ccgtcaggca gacggcaaaa tcgtactgtt ccgtccgact
     3061 gccaacatcg cgcgtatgcg gcaaagtgcg gacattttgc acctgccgcg ccccgaaacc
     3121 caagcctatc tcgacgcgct aatcgaattg gtcaaacgcg ccgccgatga aattcccgat
     3181 gcgcctgccg ccctgtacct gcgtccgacc ttaatcggta ccgatcccgt catcggcaag
     3241 gccggttctc cttccgaaac cgccctgctg tatattttgg cttcccccgt cggcgactat
     3301 ttcaaagtcg gctcgcccgt caaaatttta gtggaaaccg aacacatccg ctgcgccccg
     3361 catatgggcc gcgtcaaatg cggcggcaac tacgcttccg ccatgcactg ggtgctgaag
     3421 gcgaaagccg aatacggcgc aaaccaagtc ctcttctgcc cgaacggcga cgtgcaggaa
     3481 accggcgcgt ccaactttat cctgattaac ggcgatgaaa tcattaccaa accgctgacc
     3541 gacgaatttt tgcacggcgt aacccgcgat tccgtactga cggttgccaa agatttgggc
     3601 tataccgtca gcgaacgcaa tttcacggtt gacgaactca aagctgcggt ggaaaacggt
     3661 gcggaggcga ttctgaccgg tacggcagcc gtcatctcgc ccgttacttc cttcgtcatc
     3721 ggcggcaaag aaatcgaagt gaaaagccaa gaacgcggct atgccatccg taaggcgatt
     3781 accgacatcc agtatggttt ggcggaagac aaatacggct ggctggttga agtgtgctga
     3841 tgctttaaat aaaaaaatgc cgtctgaaac ccgtttggcg tttcagacgg cattttcgcg
     3901 ttcggaccat ttccgctgca cctgcagcaa gtcgggcatt tccctagaaa tacgcttcag
     3961 acggcaaacc gccgcccgaa accgatacgc ggtacggacg gcgcacggat ttgaaaacgg
     4021 cggattatcc ctcggtgctc aaggcattaa tgctgtaacc gccgtcaacg taagtgattt
     4081 cgccggtaat gccggacgac aggtcggaca gcaggaaggc ggcggtattg ccgacttctt
     4141 caatggtaac gttgcggcgc agcggattgt gggcggcgac gtgtcccaaa agtttgccga
     4201 aatcggcgat accggaggcg gcaagcgttt taatcgggcc ggcggaaatg ccgttgcagc
     4261 ggatgccttc tttgcccaga caggcggcgg taaaacggat gcccgcctca aggctggctt
     4321 ttgccatacc catcacgttg taatttggaa tcgcgcgcac cgcgcccaag tagctcaggg
     4381 caacgatggc ggagtttctg ccgcgcatca tcggacgggc ggcttttgcc aatgcgggca
     4441 ggctgtatgc ggaaatttcg tgtgcggtgt tgaacgcttc gcggctgatg ctgtcgagga
     4501 agtcgccgct caaggcttct ttcggcgcga agccgatgga atgcaccaaa ccgtccaagc
     4561 cgtcccaatg tttgcccaag tcggcgaaca cttggttgat ttcgtcgtcg ctggcgacat
     4621 cgcagcggaa tacgagttcg gaacccaatt ccgccgccat tttgcggacg cgctcttcca
     4681 gtttgtccac aacgtaggta aacgccagtt ccgcgccttg ttcgcggcag gctttggcga
     4741 tgccataagc gatggaacgc tcggaaatca tgccggtaat cagaattttt ttgccttgca
     4801 gaaaacccat tttgtatcct tcaaatagtg ttcggttcat tctaaaccgt tgattatagc
     4861 aaattgtccc tgtttctgtg ttttcacgtt gcagcatgca aacggcaatg ccgtccgaag
     4921 cggatttcag acggcatcgg acgtttcaaa tacggtttaa ggcatcagat gccgcgcaac
     4981 aattcgttca cgctggtttt cgcacgggtt tgcgcgtcca cgcgtttgac gatgacggcg
     5041 cagtaaaggc tgtggctgcc gtctttggaa ggcatactgc cggacacgac gaccgaaccg
     5101 gccggtacgc ggccttggta gatttcgccg gtggtgcggt cgaagatttt ggtggattga
     5161 ccgatgaaca cgcccataga aatcacgctg ccttcttcga caatcacgcc ctcaacgatt
     5221 tcggaacgcg caccgatgaa gcaattgtct tcaataatgg ttgggctggc ttgcaggggt
     5281 tcgagtacgc cgccgatgcc gacaccgccg ctcaagtgga cgtttttgcc gatttgcgcg
     5341 caagagccga cggtcgccca agtatcgacc atcgcacctt cgtcaacgta tgcgccgatg
     5401 ttgacgtaag acggcatcag cacgacattt ttcgccacaa agctgccgcg tcgggcaacc
     5461 gcacccggaa cggcgcggaa gcctgcgttt ttgaactcgt cttcagacca gtcggcaaac
     5521 ttggtcggca ctttgtcgaa gtatttgttc acaccgtcgt tgaggatttc gttgtcttgg
     5581 atgcggaagg acagcaatac cgcttttttc gcccattcgt taactttcca ttcgcccacg
     5641 cccaagcgtt cggcgacgcg cagtttgccg gaatcgagtt ggcggatggt ttccaacacg
     5701 gcttctttaa cttcgggagt aacggtggtc ggattaatgt ccgcgcggtt ttcaaaggcg
     5761 gtttcgataa tgttttgcaa agacataatg tttccttatg tgagatgttt cgtgggacgc
     5821 ttgccggtat tttcaagcag tatgttcatt tgaaatgaat gcgctattgt gccacgcaaa
     5881 cgcatattcg tcagcaatac gcgggcggtt gccgaacatt ggcgtttcgg aatcgggcgg
     5941 gcggcagtac ctttatgccg caccgccgcc tttcagacgg cattcgcggt aaaacgccat
     6001 cagcccttcg gtcgaggcat cgtgtacgga cgtgccgcct tccagttcgc cgatgatggt
     6061 ttttgccaac tgtttgccgt attccacccc ccactgatcg aaggggttga cgttccatat
     6121 cgcgccttgg acgaaggttt tgtgttcgta agccgccatc agcataccca aattgtaggg
     6181 cgtgaggcgg tcgagcagga tgctgttgct ggggcggttg ccagggaact ctttgtgcgg
     6241 cgcgaggcgt tcgcgttccg cttcgggcaa atctgccagt tcggcgcgtg cttcgtccaa
     6301 ggttttgccc ttcatcaggg cttccgcttg ggcaaaggcg ttggcaacgg taaaacggct
     6361 gcgtccgtcc tccacgccct gcgccgtcat cgggacgata aagtcgcagg gaatcaggcg
     6421 cgtgccttga tggagcagtt ggaaataggc gtgctggcag ttgacccctt caccgccgaa
     6481 cacgatgccg cccgttttgc acacggcggg actgccgtct gaagcgcggc ttttgcccaa
     6541 actctccata tcgagctggt tcagccacac cggcagcagg cgcaggttgt ggctgtacgg
     6601 aacggcggtc tgcccgtccg cgtgttggaa attgttatac cacacggcaa tcagtgccat
     6661 cagaacggga atgttgcggc gcggcggcgt gtggaaaaaa tggctgtcca tcgcgtgcgc
     6721 ccccgccaac aactcgcgga aacgcgcccc gccgaccgca accatcacgg gcaaaccgac
     6781 gggcgaccag acggaatagc gtccgcccac ccagtcgtac atcgcaaaca cgcgttccgc
     6841 cgcgatacca aaagccgcag ctgccgcagt gtcggcagac accgcgcaaa aatggcacgc
     6901 cgtttcggat tccgagaacc ccgcaccgcg ataccacgcc ttgactgcct gtgcattgag
     6961 cagggtttcc ggtgttttga aggacttgct ggcaacgcaa aacactgtcg tttcggggtt
     7021 cagacggcat aaaaccgcat ccaggcaggc aggatcggcg ttggcggcaa aatggacggt
     7081 gatatgccgt ctgaacggct caagtgcctg cacgcacatt gccggcccga ggtcggatcc
     7141 gcctatgccg atgtggacaa aatccgtaat ccgttttccg gttgtcccct gatacgaacc
     7201 gtcgtccaaa ctgtgtgcaa actccaacgc acgatttaac tcgcggcgga tttcgggcaa
     7261 cacgtccctg ccgtccgcat aaacggcatc cgcaccgtcg ggtaggcgca aagccgtatg
     7321 cagcgcggca cgcccctcgc tgccgttgac tttcgcaccc gtccgcaaag cacgcatttt
     7381 cccttccaaa tccgccgtct ccgcaagacg gcagagcagt tgcagcgtat cttcgcccaa
     7441 acggtttttg ctgtaatcga acaacatccc gtccaaacgc tcatgcatac gctcaaaacg
     7501 gtcgggttcg caggcaaagc ggtcgcgcaa aaggacatga cgcgtatcct gataatggcg
     7561 ttcgagcgca taccatgcac gggtaaaagc attcatctgt tttccttgat ttttcagaac
     7621 cggattaaaa tgtagcagaa tgtagtttaa caaacggcag cggctttggc gaatctccgg
     7681 aacaccgccc gccgcgtcca atagcacctt atttccatat ggcgaaaata tagtgaatta
     7741 acaaaaatca ggacaaggcg gcgagccgca aacagtacaa atagtacgga accgattcac
     7801 ttggtgcttc agcaccttag agaatcgttc tctcttgagc taaggcgagc caacgctgta
     7861 ccggtttttg ttaattcact ataccctccg gccggcattt gacccgcaac aatatcctgc
     7921 aagatttatt gtgtacgcat aaatgccgga cagccgccta aataaggtat aatcgcatcc
     7981 gattctgtcc cgtttgccgc ccgcaggcgg gatgtcggcg gttttcatct taccgggaac
     8041 acgccggtac tgttttttaa cgatgtttct tacattttat tccaattact ttacggggct
     8101 agaatatggc taaaaacgga ggattttctt tgttcgcaaa gaaagaaaaa cgctttatct
     8161 ttgaaggcag gcattccgcc tccgacaaac tggtcaacgg cgaagtatcc gcgtttaccg
     8221 aagaagaggc gcgcaaaaaa ctggcaaaac gcggcatccg cccgttgcag attacccgtg
     8281 tgaaaacaag ctccaagcgc aaaatcacac aagaagacat caccgttttc acccgccagc
     8341 tgtccacgat gattaaagcg ggcctgccgc tgatgcaggc atttgaaatc gtggcgcgcg
     8401 gacacggcaa cccgtctatg acggaaatgc tgatggaaat ccgaggcgaa gtggaacagg
     8461 gcagctcgtt gagccgcgca ttctcaaacc acccaaaata tttcgaccgc ttctactgca
     8521 atctggttgc ggcgggcgaa acgggcggcg tattggaaag cctgctggac aaattggcaa
     8581 tttacaaaga aaaaacccag gccatccgca aaaaggtaaa aaccgcactg acctatccgg
     8641 tatccgtcat cgccgtcgcc atcggtttgg tattcgtgat gatgattttc gtactgcccg
     8701 cctttaaaga agtttacgcc aatatgggcg cggagcttcc cgcactgacc caaacagtga
     8761 tggatatgtc cgactttttc gtctcatacg gctggatggt gctgatcgca ctgggctttg
     8821 ccatatacgg cttccttaaa ttgaaggcgc gttcgattaa aatccaacgg cgtatggatg
     8881 ccatactgct gcgtatgccg attttcggag acattgtccg caaagggacg attgcccgct
     8941 ggggcaggac gacggcgacg ctgattgcgg caggcgtgcc tttggtcgat gtattggact
     9001 ccactgccgg cgcggcgggc aatttaatct atgaagaagc cacccgggaa atccgtacgc
     9061 gcgtcatcca aggtttgtct atgacttcgg ggatgcgtgc gacggaactg ttccccaata
     9121 tgatgttgca gatgtcttcc atcggcgagg aatcgggttc tttggacgat atgctcaaca
     9181 aagccgccga attttacgaa gacgaggtgg acaatgcggt cggcaggctg tccgctatga
     9241 tggagccgat cattatcgtg attttaggct tggtcatcgg cacgcttctg gtcgccatgt
     9301 atctgccgct gtttaatttg ggcaacgtgg tcgcctgatt tgccgcacag atccggcgcg
     9361 gattcgctct gcgccggttt gtttttgttt tgaatacatt gagaacaaaa tatgtctgat
     9421 ttgtctgtat tgtcgccgtt tgccgtgcct ttggcagcgg tgttcgggct gctggtcggc
     9481 agtttcttaa acgtcgtcat ttaccgcgtg ccggtcatga tggaacgcgg ctggacggta
     9541 tttgccaaag aatatttaaa cctgccgctg accgaagagg aaagccgtac cttcaacctg
     9601 atgaaaccgg attcctgctg tcccaaatgc cgcgtgccga tacgcgcgtg gcagaacatc
     9661 ccgattgtca gttacctgct cctgcgcggc aaatgcgcct cctgtcaaac caaaatcagc
     9721 atacgttatc ccttaatcga gctgctgacc ggcgtattgt tcgggctggt cgcctggcaa
     9781 tacggctggt cttggattac gctgggcgga ttggtactga ccgcgtttct gatttccctg
     9841 acctttatcg atgcggacac ccaatacctg cccgactcga tgacactgcc cttgatttgg
     9901 ctggggctga tatttaattt ggacggcggc ttcgtgcctt tgcagtctgc cgttttaggt
     9961 gcggttgccg gctatggttc gatatggctt ttatgcgcgg tatataaact gattaaaggc
    10021 gaaatcggta tgggcaacgg agatttcaaa ctgattgccg cattgggcgc gtggctcggc
    10081 atatccgcat tgcccgtgct gatttttgtt tcctcgctga tcggtttggt cgcggcaatc
    10141 gttatgcgcg tcgccaaggg gcagcatttt gccttcggcc ccgcactgac agtttcgggc
    10201 tggataattt ttacggcaaa cgattccgta tggcgggcgg tcaactggtg gctgacccat
    10261 ccggtgctgt aagatgacgg tatgggtcgg actgaccggc ggaatcggca gcggcaaatc
    10321 ggcagccgcg caatgttttg ccgatttggg cgtgccgcgc atcgatgcgg acgcggcggc
    10381 gcactcgctg acggcttcag acggcatcgc cctgccggaa atcaggcggc tgttcggcga
    10441 caccgttttc gacacacagg gtttgttgcg acgcgacata ttgcgtaaag aagtctttgc
    10501 ctccccatcg cgaaaagcct tgctcgaatc cgtgatgttg ccgctgattt tctcagaaat
    10561 caaaaaacag caagaaacct ttaccgatgc ggcttacggc attgtcgaaa ttccgctgct
    10621 gacggaaaag cgtcaattta tcagcctgat acggcgtgtc ctgaccataa gcgcaccttt
    10681 ggaaaaacgt atcggcaggg tgatggcccg cagcgggctg acgcgcggcg aggtggcgga
    10741 catcatcagc catcaggcat ccgaatccga acgcctgctg cttgcagacg atgtgctgct
    10801 caatgacggc agcctcaaaa gcctgcgtga gaaaacaatg cgcctgcacg cgttttattc
    10861 agggattttc gcctcaaaac caacacaagg aaaacacaat gactgaatcg cggcaaacac
    10921 gccttcaagt caaatgtccg acctgtcaaa cagcagtagt atgggaaccc gaaaacgcat
    10981 tccgcccctt ctgttcgcaa cgctgcaaac tgatcgactt gggcggatgg gcagacggga
    11041 aatatacggt ttccggccaa acggaaagtt tgccggaaat atccgaaccc gacatggcat
    11101 accgctgacc gccccgcctt cccggcaaac accctgaaag tcaaatgccg tctgaaacaa
    11161 acacgcttca gacggcattt tcattctcaa acctaatcgt tggtatttgc cgttacctct
    11221 tccaatgaag taatgccctg cataactttc aaaataccgg cccggcgcaa atccaccata
    11281 ccctccttat aggcaacgtc caaaatatcc acttccgtac cgttgttcat aatcacacgc
    11341 tgcatttctt cgctgatggg cataacctca tacacgcccg cacgcccctt ataaccctgc
    11401 ccccggcaac ggtcgcaacc gacggcgcgg taaagtttcc aatcttttgc aaggtcctca
    11461 tcggtgaagc cgacttcctt caaagcagag gcagacgggc gttccacttc ctgtttgcag
    11521 ctcgaacaca gcctgcgtaa aagacgctgc gccataatca ggctgaccga actggcaata
    11581 ttaaacggcg cgacacccat attcagcata cgcgacaacg tcgccggcgc attattggtg
    11641 tgcagggtgg aaaacaccat atgccctgtt tgtgccgcct taatcgcaat atcggcagtt
    11701 tccaaatcac gaatctcacc gaccataatg atgtccgggt cctgacgcag gaaagacttc
    11761 aaagcagcgg caaaagtcag gccctgctta tcattgacgt taacctgatt gatgcccggc
    11821 aggttaatct cggcagggtc ttccgccgtt gcaatattta ccgactccgt attcaaaata
    11881 ttcaaacagg tatagagcga caccgtctta cccgaacccg tcggaccggt taccagcacc
    11941 atcccgtagg gacggtgaat cgcttccaac aacaattttt tctgaaacgg ctcaaaaccg
    12001 agctggtcga tgttcaaaga cgcggcatcg gaattcaaaa tccgcatcac gaccttttcg
    12061 ccaaacagcg tcggcaatgt gctgacacgg aaatcgacag gcttgccgcc cttttgaaag
    12121 gtcagctgca tcctgccgtc ctgcggtatc cgtttttcgg aaatgtccaa acgcgacatt
    12181 accttaatcc gggaagcaag ctgccccctt accgcaatgg gcggctgaac cacctcgcgg
    12241 agctgcccgt ccacacggaa acggatacgg gcattgtgtt cgtaaaactc gaaatggatg
    12301 tccgatgccc cgctgcgcaa ggcatccgac aaagtcttat ggataaacct cggaacaggg
    12361 ccgtcttctg cctcctcgtt gtcgatatac agggtgtggc tttcctcttc ctcctgcccc
    12421 tccccaagct cctgaagcag cgatgtcgaa cgcgaaccca cccaatcgag caaacccgcc
    12481 aactggtcat cctcgacaat gaccaactca acctcaatcc ctgcggcaga aacggttttc
    12541 tgaatttgcg gcatctgtgt cggatcggaa accgcaaaaa atactttgtc gccccgacgg
    12601 aaaaccggca cacagtggaa ctccaccatc tgctcctccg tcaacacccc catcagcacc
    12661 ctgtggcgcg gataatgacg caaatcaaga atcgaataac tgaacaccct tgcaatcaat
    12721 gccgcaagcg acttgggcga aatgacgccg tctgaaaaca gtatcggcaa cacttcctta
    12781 cccgcctgcg actccttgta gtaatgctcg gcctgctcaa cagtaaccac ctggttttga
    12841 accagaatcc tcagcaaacc tacgctcata cgaccttatc cccaaattta ttcattgtta
    12901 tacctgtaca gcttttatag tggattaaat ttaaaccagt acggcgttgc ctcgccttag
    12961 ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactatct
    13021 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atcataaaac
    13081 aacagatttc caagccggaa catcttttac ggagcctgaa aatcatttca tgattctacc
    13141 gtcctaaagg tcggtttttc aagcaggaag aaaaagtttt cagatggcaa aaaagccctc
    13201 caacactgaa aggccttata tcggaaactt cccgcaacac gggaaacaga caaatgaaat
    13261 cgtcaaacct cgccaacagg aatcgaacct gtattttacg cttaggaggc atacgttcta
    13321 tccgttgaac tatggcgagc cgaaatgaaa aggagatttt aaccctttcc gacgacaaaa
    13381 gacaagatgc cggccgcaat cagcggcccg acagggatac cgcccataaa tgccaccccg
    13441 ataaccgtcc cgattaacag ccctgtaatt aaaacaggct gctgtcccat caaaggcacg
    13501 ccgcgtcccg ccagccaagc cacgaaaata ccgataaaaa cggcggatat cattttaaaa
    13561 ttcaaaaatt cggcaacggg aggaacctgc gcctttcctg aaaccaacgg actcaaaacc
    13621 cctatggtca aaagaatgat accgagattc aacccatgct tctcgaccaa cgggacaaac
    13681 tgtaccaatg ccgtctgctg catcagcagc aatatggttg ccgagatggt aatcgaattg
    13741 ttgttgctga ccacccccaa cagaatcagc gtaaccagaa acaggggaac aaaactgaaa
    13801 ttcatcgcct caagacaccc tgaccaaaga ttccttcgcc agctgaatca tggcatcctt
    13861 cacttcgctg tcgggcaggg catccaaggc ggtaacggca caatcgactg ctttgcgcgc
    13921 ctcgcctatc gaatatgcca aagcatccga acggacgaca taatcgtgga ttttctcaaa
    13981 atagctgcga tctgcatttt ccaaagcagt acgcacatcg ttcgcaacct gttcggaacc
    14041 ctgacgcatc agataaatca aaggcaaagt cggttttcct tccgccaaat cgtcgccgac
    14101 gtttttgccg gtttcttcgg tttcgccaga atagtccagc acatcgtcaa taatttggaa
    14161 tgccgtaccg acatacatac cgtagtcttt caacgcccgt tcgtgttcgg gggaagcctt
    14221 gcccaaaatt gcgccgacct gcgccgccgc ttcaaacaat tttgccgttt tatattggat
    14281 gacttggata tattgttctt cggtaatgtc cgtattgccg atgttcatca gctgcatgac
    14341 ttcgccctcg gcaataatgt tggttgcatc cgccatcact tccaaaacgc gcatactgcc
    14401 cgaggcaacc atcagttgaa aggcgcgcgt gtataaaaag tcgccaacca acacagccgc
    14461 cgcattgccg aacagattgt ttgccgttgc ccgcccacgg cgcaaatcgc tttcatcgac
    14521 gacatcgtcg tgcagaagcg tcgaagtgtg gataaactcg accatcgccg ccagcgaata
    14581 cagtttctcg tcatcataac cgaccgcctt acccgccaaa atcgtcataa tcggacgcag
    14641 gcgtttgccg cccgcgctga tgatatatgt accgatttgc gaaatcagtg cgacatcgga
    14701 ttgcaccgca cggttgatga cttcattgac tttggcaagg tcttcaggca gatgtcgctg
    14761 gaaatagggc agattctcga gcatggcaga ctctcaggtt tacgtttccg tcaaacggca
    14821 gataccgctt gaaagctgat tcaaatagcg ggcaattata tcagcaaagc catcagggta
    14881 catcttttca gacggcatat ggttatccga accgcgcaaa ctttgacaaa acaggcaaat
    14941 aacaatacaa tgccgagttc cgcacaatgg tgtgcggata ttaaccataa cccttatgga
    15001 gttgagtatg tacgcggtcg taaaaactgg cggcaaacag tataaagttt ccgtcggcga
    15061 aaaattgaaa gtagaacaga taccagccga actcgacagc caaatcgaac tgaccgaagt
    15121 tttgatgatt gctgacggcg aatctgtaaa agttggcgca ccctttatcg aaggtgcaaa
    15181 agtaacggct aaagtagtgg cacacggtcg tggcgaaaaa gtccgcatct tcaaaatgcg
    15241 ccgccgcaaa cactaccaaa aacgccaagg ccaccgccaa aatttcaccc aaatcgaaat
    15301 cgtggcaatc gcctaacctt aaaacttagg agcattacaa atggcaagta aaaaagcagg
    15361 cggcagcacc cgcaacggtc gcgattcaga agccaaacgc ttgggcgtta aagcctacgg
    15421 caacgagctg attcccgcag gttccatcat cgtccgccaa cgcggcacca aattccacgc
    15481 aggcgacaac gtaggtatgg gcaaagacca cactttgttc gccaaagttg acggttatgt
    15541 cgaattcaaa accaaaggcg cgctgaaccg caaaaccgta agcatccgtc cttacaccgg
    15601 ttctgaagaa taattcaacc gaacaggaag ccgcatcttt tcacgatgcg gctttttata
    15661 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa
    15721 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
    15781 acgccgtact ggtttaaatt taatccacta tacatacccc acacccgatt tgatacacat
    15841 taagctgaaa gtaaaaatcc gcatacaccc tcccctgcat atttcttcaa cagtgggttt
    15901 tgatataatc gcctatctgt tacagatagt tcaaacggca ttcaaaccct tacaaatacc
    15961 gtctgaaatc atcggcatcc gtctatgcaa aggatatttt atgagtttac acagcgacat
    16021 cctcgtcgtc ggcgcaggcc cggcgggttt aagttttgca gccgaacttg ccggcagcgg
    16081 tttgaaagtt accctgatcg aacgcagccc actgactgtt ctgcaaaatc ctccatatga
    16141 cggacgcgaa atcgccctga cccatttttc ccgcgaaatc atgcagcgtt tgggtatgtg
    16201 ggacaaaatt cccgaaaacg aaatctaccc tttgcgcgat gccaaagtgc tgaacggacg
    16261 ttccgactac cagctccact ttccccaacc gaccgaggcg cgcggcgagc ctgccgactg
    16321 tttgggctat ctgatttcca accacaatat ccgccgcgct gcctacgaag tcgtatccca
    16381 actcgacaac attaccattc tgaccgatac ggccgttaaa gaagtcaaaa catctgacaa
    16441 cgaagcacaa gtcattttag aaaacggcaa aatcctgacc gcccgcctgc ttttggcggc
    16501 agacagccgt ttttcgcaaa cccgccgaca actcggcatt tcttcagata tgcacgacta
    16561 cagccgaacc atgttcgtct gccgcatgaa acatactctt tccaaccaac ataccgctta
    16621 cgaatgcttc cattacgggc gcaccatcgc actgctgccg ctggaagaac acctgaccaa
    16681 taccgtcatt accgtcgata ccgataaaat caacagcgtt caaaaccttt cgcccgaaga
    16741 attggcggca agcgtaaaag aacagctcaa gggcaggctc ggcgatatgg aattggtcag
    16801 cagcatccac cattaccccc ttgtcggtat gattgccaaa cgtttctacg gcaaacgcag
    16861 cgcgctgatt ggcgatgcgg cggtcggtat gcaccctgta acggcacacg gcttcaactt
    16921 gggtctttcc agcgcagata ttctggcaaa actgatactc gaagccgaac aacgcggtca
    16981 ggacatcggc gcatcttcct tgttggaaaa atacagcaac aaacatatgc tgcacgccca
    17041 tccgctttat cacggaacca atatgatgct gaaactgttt accaacgaaa ccgcgccggc
    17101 aaaactgctg cgcggtttgg tattgcgtgc cggcaacaac ttcccgccgc tgaaaaagct
    17161 gattaccaaa caactgaccg gttgaaaact gccgaaatgc cgtctgaaac gtttcagacg
    17221 gcatttttg
//
LOCUS       DAXIHS010000041        16992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXIHS010000041 DAXIHS010000000
VERSION     DAXIHS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.041"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(197..1366)
                     /gene="metK"
                     /locus_tag="ACFOF2_001180"
     CDS             complement(197..1366)
                     /gene="metK"
                     /locus_tag="ACFOF2_001180"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC7107502.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1549..1659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1712..2608
                     /locus_tag="ACFOF2_001181"
     CDS             1712..2608
                     /locus_tag="ACFOF2_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC7107503.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2652..3716)
                     /gene="tsaD"
                     /locus_tag="ACFOF2_001182"
     CDS             complement(2652..3716)
                     /gene="tsaD"
                     /locus_tag="ACFOF2_001182"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC7107504.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPIHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            3723..3848
                     /locus_tag="ACFOF2_001183"
     CDS             3723..3848
                     /locus_tag="ACFOF2_001183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107505.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3833..5020)
                     /gene="ccsB"
                     /locus_tag="ACFOF2_001184"
     CDS             complement(3833..5020)
                     /gene="ccsB"
                     /locus_tag="ACFOF2_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC7107506.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRTEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5013..6965)
                     /locus_tag="ACFOF2_001185"
     CDS             complement(5013..6965)
                     /locus_tag="ACFOF2_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC7107507.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7242..7865)
                     /locus_tag="ACFOF2_001186"
     CDS             complement(7242..7865)
                     /locus_tag="ACFOF2_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC7107508.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8071..8700
                     /gene="yihA"
                     /locus_tag="ACFOF2_001187"
     CDS             8071..8700
                     /gene="yihA"
                     /locus_tag="ACFOF2_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC7107509.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8821..11217)
                     /locus_tag="ACFOF2_001188"
     CDS             complement(8821..11217)
                     /locus_tag="ACFOF2_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC7107510.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11369..12484
                     /gene="pilM"
                     /locus_tag="ACFOF2_001189"
     CDS             11369..12484
                     /gene="pilM"
                     /locus_tag="ACFOF2_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC7107511.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKLLYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12487..13086
                     /locus_tag="ACFOF2_001190"
     CDS             12487..13086
                     /locus_tag="ACFOF2_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC7107512.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13087..13734
                     /locus_tag="ACFOF2_001191"
     CDS             13087..13734
                     /locus_tag="ACFOF2_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC7107513.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13752..14297
                     /locus_tag="ACFOF2_001192"
     CDS             13752..14297
                     /locus_tag="ACFOF2_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC7107514.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14316..16601
                     /gene="pilQ"
                     /locus_tag="ACFOF2_001193"
     CDS             14316..16601
                     /gene="pilQ"
                     /locus_tag="ACFOF2_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC7107515.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4587 a         4820 c         3855 g         3730 t
ORIGIN      
        1 aatttaaacc agtacagcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
       61 tgaagcacca agtaaatcgg ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt
      121 cctgattttt gttaatccac tatataaaaa tgccgtctga actgttcaga cggcattttt
      181 gattttcaaa ccggaattac agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc
      241 gctcccaagt gaactcaggt tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt
      301 aaatcgggcg caagagatcg agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt
      361 cgcgaactaa ggtaatcagt ttttcctcgc tgattttgcc tgtgccgaaa gtgtcgatgg
      421 aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg
      481 ccaaaccggc ggcgacgatg tttttcgcaa cgtagcggca ggcgtaagcg gcggaacgat
      541 ccactttgga cgggtctttg ccggagaacg caccgccgcc gtgcggagcc gcgccgccgt
      601 aggtatcaac gatgatttta cggccggtca aaccgcagtc gccttgcggg ccgccgatga
      661 cgaagcggcc ggtcgggttg atcaggtatt tggtttcgtc ggtcagcagt tcagacggca
      721 ggacgggctt gataatctgc tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg
      781 ggtcgtgttg cgtagagagg acgacggtat caatgcgttt tactttgccg gattcgctat
      841 cgtaaaccac ggtcagttgg gcttttgcgt cgggacgcag ccaaggcagg aggccgtctt
      901 tgcgtaattc gctttgacgc tgcatcaggc ggtggctgta atagatggca aacggcatca
      961 gggtcggggt ttcgtcgcag gcatagccga acatcaaacc ttggtcgcca gcgccttggt
     1021 tcaagtcaat gccttcgcct tcgttcacgc cttgggcgat gtccggagat tgctggtcgt
     1081 agtacacgcc gaccgcgcag ccgttggcat caaagcccag ctcggaggag ttgtagccga
     1141 tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt
     1201 cgcctgccaa tacgcacaag cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg
     1261 ggtcttgcgc caagatggca tccaaaatcg cgtcggatac ttggtcggca actttgtccg
     1321 gatggccttc ggataccgat tcggaagtaa acagatattc gctcattgag attttccttg
     1381 aaaaacaaac catcttcttc agacggcatg ttgtatgaac ataatgtcga cagcgggaaa
     1441 tatagcaaaa tttccctatt cataccattc agttgagaaa tattcccatt tgaatggcac
     1501 tttggaatct ctgcccgtac gtttcttaca ggcaaaaaaa ttcccgcatc aagcgggttt
     1561 ggattgctct ggtgagccac atcggctttt caaccgtcca ccttactttt ccttttgaaa
     1621 agcaggttgg catggaattc ccaactctta atgcagcgcg gatggtagca gaaaagcctt
     1681 tccgccgcaa tacttccctt tttcagacgg catgtttcgt ttacaattcg ggctgtttcc
     1741 ccctttgcga accgccatgc acatcctgtt gaccgccctg ctcaaatgcc tctccctgct
     1801 gccactttcc tgtctgcaca cgctgggaaa ccggctcgga catctggcgt tttacctttt
     1861 aaaggaagac cgcgcgcgca tcgtcgccaa tatgcgtcag gcaggcatga atcccgaccc
     1921 caaaacagtc aaagccgttt ttgcggaaac ggcaaaaggc ggtttggaac ttgcccccgc
     1981 gtttttcaga aaaccggaag acatagaaac aatgttcaaa gcggtacacg gctgggaaca
     2041 tgtgcagcag gctttggaca aacacgaagg gctgctattc atcacgccgc acatcggcag
     2101 ctacgatttg ggcggacgct acatcagcca gcagcttccg ttcccgctga ccgccatgta
     2161 caaaccgccg aaaatcaaag cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa
     2221 aggaaaaacc gcgcctacca gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc
     2281 gggcgaagca accatcgtcc tgcccgacca cgtcccctcc cctcaagaag gcggggaagg
     2341 cgtatgggtg gatttcttcg gcaaacctgc ctataccatg acgctggcgg caaaattggc
     2401 acacgtcaaa ggcgtgaaaa ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg
     2461 tttcgatttg cacatccgcc ccgtccaagg ggaattgaac ggcgacaaag cccatgatgc
     2521 cgccgtgttc aaccgcaatg ccgaatattg gatacgccgt tttccgacgc agtatctgtt
     2581 tatgtacaac cgctacaaaa tgccgtaacg aaaataaaaa tgccgtctga acaatttcag
     2641 acggcatttt gtcatttgac gatttccgac agcggccagc gcggacggac attgaacgca
     2701 ccgacctccc tgcccttgcc caggtgcatc gcaccggcaa aggcaatcat ggcaccgttg
     2761 tccgtgcagt atgccgtcgg cgggaaaaac acgctgactt tttcggacgg atgtttcggc
     2821 ttgcctgttg gggtcgggat ttgcaccgtc atgctgccga aagtttcacg gagtttgcgg
     2881 tttgcaccga ccccgccggc gaccactacg gttctgaacc ctgtctgcaa cagggctttt
     2941 ttcactttcg ccgccaacac atcgactacc gcatcttgaa acgcacggca gatgtcgttg
     3001 cgtgtctgct caggaatgtc atccgccccg ttttccgcgc gcactttctc gacggcagtc
     3061 aaaacagcgg ttttcaaacc tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg
     3121 cgcggaaaaa caaacgcttc gggcctgccc gattccgcaa gttccgacag tttcgcaccg
     3181 cccggatacg gcaagcccag cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca
     3241 tcgacgctct cgcccaaaag cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta
     3301 tgcccgcccg aaaccagcag cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc
     3361 ggcgacagca gatgtccttc caaatgatgg atggggatga cgggcttgcc caatgccaaa
     3421 gccagtgcat tggcgtagct cgaacccgcc agcagcgcac cgcccagacc gggtccctgc
     3481 gtaaaggcaa ccgcgtcaat gtcgccatac gatgcacccg cctgcgccaa acagccttcc
     3541 gtcaacggaa caaggcggcg gatatggtcg cggcttgcca attccggcac aaccccaccg
     3601 tattcggcgt gcattgccat ttgagtgtgc aggcagtgcg cccgcaatcc tcgttccgta
     3661 tcgtaaagcg caacgccggt ttcgtcgcaa gaagactcga ttcctaatac caacatggtc
     3721 tgatgccgtt aaaaactgaa aaacgtattt tagcggattt cggcacgact gccgtatccc
     3781 aaaaacggaa catgccgtct gaagaccgtt cagacggcat cgtcgcaccg tatcaaagcg
     3841 ttccgtaaga atgcagcccg ctcaaaaaca tattcacgcc gataaaggca aatgcggtta
     3901 cgaacaaacc gataatcgcc caccacgcca gcactttgcc gcgccaaccg gcaaccagcc
     3961 gcaagtgcag ccaaacggcg taattgagcc agacgatgaa cgcccacgtc tctttcgggt
     4021 cccaactcca atagcgtccc caagcatccg ccgcccacag cgcacccaaa atggtggcaa
     4081 tggtaaagaa cagaaagccg acggcaatcg ccttatacat aacttcctca atcaacacgg
     4141 attgcggcag ccacgatttc ccgccttttc cttccgtacg cagggaaacc agttcggcaa
     4201 taccgagcat cgcggaaatg caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga
     4261 ttttcatcca ccaagattgc aatgccggaa tcagcggctg gatggtatgc gcctcgcggg
     4321 acacgctgta ccacaagaca aatccaacca cgaccgccat aaagccgaac acgaagccgc
     4381 ccaatttctg tatggcgaac ttaccttcat aataaagata catcagcgcg gtaatcacca
     4441 aaaacaggat gaacacttca tacaggttgg aaaccggaat atgccccgca tcgggacgga
     4501 gcagatagct ttcgtgccaa cgtaccagca gaccggtaaa gcctgctacg gcagacaccc
     4561 atgcaaacac ggttcccata cccaacagcg tgttggtcgg cacatttttt acgcttgcca
     4621 aaaccgcgcc cgaaatatag gcgaacaggg cgaagaagac aaaggcgcac tgccacataa
     4681 tcgccgactg gctgctgagg aaataccgca acaggaaaat ctctgccgat ttgatgttgc
     4741 cgccatacaa accgacggcg gcataggcaa gcaatacgct taaaggaaca aaccagcgca
     4801 tcggcttgaa aaaccagccc aaaaatacgg caataccggc actcgcccac aacatgaccg
     4861 tttcgtaaat gtccatatgc ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa
     4921 ccagcacggc aaatacccaa tcccaaagat tcagattgct gatcaaagac ttctgaatca
     4981 gcagctcgtg ttccggaagg gttttatagt gttcagtcat gattcaagtc cttgccgagc
     5041 cgttgcagac tctcgacgtg ttttggaaat tccttctgca aatcccgttc gctgcgggcc
     5101 gaagacatgg caaaacggat tttgccgtct gaaaacaata cccacgcccg tttttcgcgc
     5161 acataaaaca tcaataccgt acccaatacc aacagcaccg agccgagata gaccaaaagc
     5221 gcacccgggg aacgggtcat ctgcaaaccc gacgaacgca cctcggaaaa cccatcaagt
     5281 tgcagcagca taggcgcggg atattcggtc aaacccgtgt acgcatccat actgtgcagc
     5341 aggaaacggt tccgcgcttc atcctgctgc cattcgggca agccgtaccg gcgtatggtt
     5401 tcatccaaag cagcgttcat cacgccgtaa agcatttcgt agaaataacc ctgcatctta
     5461 tcctgctgct ctttcggaat attggacgta ataaattcgt ccaatcccaa atagcctttt
     5521 tgtgcaaaga tgttcagcgt gttttccgca gccagcatga attgttcgcg gatttcggca
     5581 ggtgcgtctt tggttgcgtc ggcaaccaga cgtttgcgcc cttccccatc tttcaaaaac
     5641 tcgcgcaatg ccataaaggt gtccgctttc aactgcttgt ccaaggggat acgcagccag
     5701 cggtattgct gctgcaagcc gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc
     5761 aaaaccggca gcatatagtt tttatattcg accgcctgcc ctgccgcatc acggatacgg
     5821 taaacaatgg aagggccgat attggtgtat tttttacctt cctgagtaac ggcgcggaca
     5881 tcgttcagcg tggatttcag gcttttttcc cgttccgcgc cctcgctcat gtcctccaca
     5941 ttcatagaag tgaactgatc gaactcaaga cgatatttgt gtttgccaat ttccaacgga
     6001 aactggtgta tggatgttgc cttcaacacg acaggctcgc gcgaagcatc acccaaattc
     6061 cacgccttga atgtcaaatc cgaaccgccg tcggcaaaac tcgcctgata aatcgtgatg
     6121 ccgtgcaagg tcaaaggatg gttcacgcgg atggtgcgct cgagtttctc accggttgcc
     6181 ttatccgtta cttcaatatc actggcaaaa tcgcgcggca tacccgtatt gtaaaaatcg
     6241 atatggaatt ttttcagttt gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg
     6301 gcattcagga aaaccacatc cgcactctgc ccctcggaaa tattgacgtt gcccctaaat
     6361 gagagattgg acgcacccaa aatactttcg ggcttgaaat ccttggcata aaccgcctga
     6421 ttgtccggaa caatccgacc ggtcagcata cccagtttca acagcaggtt actgtctatc
     6481 aacccgccca ggcaaatgac aatcaaagca acatgggcaa agatatagcc ccatttgttc
     6541 attgtgcctt ttttggcggc aatcagaacc gacccgtctt cacggttaat ggtttttccc
     6601 tgaaaacctt gtacttccag ataacgtttg gcaacctcgg gcgcaatttt tacatccaac
     6661 agcgaagaat ggcgcatcgc cgccagagat ttttctttaa ccttttcccg aaaagacttc
     6721 atttcgcgcc agaacggcgg cacattgcga atcaggcaca aactggtaga aaccaccaaa
     6781 aacatcatga taacgacaaa ccatgccgaa gcatagacgt catacagtcc cagaaaacca
     6841 aaaatctgcg cccaaaacga tccgaatttg accaaataat ccgtctgcgg ctggttttgc
     6901 tgcaacaccg taccgataac cgatgcaata cccagcagac tgagcaaagc gactgcaaag
     6961 cgcatggagc tgaaaaaagc gaaccacgga cgggaaagaa gtgggggaga tctacgggat
     7021 ttactcattg tgtgtttatt ccgccatcag aaatatggga aagcagaatt gggcaaacag
     7081 aaaacaacgt cccgattcta ctgtcttgat gctttttatt tcaagacaat gaagacagcc
     7141 tgcatcgatt ccaacggttg cgattgaaaa aacttatcgc agaattgcct gaagccgtct
     7201 gaaaactttt cagacggcct ctaaaacaga ctattgcgga attaacgcaa accttggata
     7261 aagttggcga ccgctttcaa atcttcttca gacatacggt ttgcaatatc ttccatgatg
     7321 gtatttttac gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctgatgc
     7381 tgaccgccca aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg
     7441 ctcggaccgt ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag
     7501 attttcgcac ccaattcggg attttcctta ggattggctt caccggattt gggctgctgt
     7561 ttggcataga atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt
     7621 ttcatcacag ctgccgaacc gtgggtgcgt ttaccgtcgc ggatgccgat agtttgatga
     7681 tagatgtaag cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg
     7741 tctgctgcat ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct
     7801 gctttgggag aggcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt
     7861 ttcatggagt gctcctgatt acagcattgg ataacgcaac aatgctcttt ttatattcaa
     7921 atacgggatt tttgacccga ttaaaaccga tgattctgta aacgtgttat tctatactaa
     7981 atttacatta aattaccact gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga
     8041 caaacggcgg cggaaaacaa ggatatgccc atgaaccttt ttcaaaacgc caaattcttc
     8101 acgacggtca accaccttaa agacctgccc gacacccctc tcgaaattgc ctttgtcggc
     8161 aggagcaatg ccggaaaatc cagtgccatc aataccctga ccaaccatgt ccgtcttgcc
     8221 tacgtttcaa aaacacccgg acggacgcag catatcaact tcttcgagct gcagaacggc
     8281 aattttatgg tcgatttgcc cggctacggt tatgcccaag tccctgaagc agtacgcgca
     8341 cattgggtca atctgctcgg cgactatctg caacagcgca aacagcttat cgggctggtt
     8401 ttgattatgg atgcccgcca tcctttaaaa gaactcgaca tccgtatgct ggattttttc
     8461 cacacgaccg gcagaccggt tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac
     8521 gaacagataa aaaccctgtc ccaagtcaaa aaactgctca aaccttattc cgacaggcaa
     8581 aacatcagcg tacagctgtt ttccagcctg aaaaaacaag gtattgacga ggccaaccga
     8641 actgtcggaa gctggtttga cgcagcagat gccgccgctt cctctccaga ggaaaactga
     8701 ccccaattat acggaaaccg tatttccccc acttgaccga ccgcaaacat ttaaaaaatt
     8761 gccactgcca aatctaaaat gccgtctgaa agacttttca gacggcattt tgcggagtct
     8821 ttaaaacaga gaatccaact gctgctgttt ggaaccagta ttactcggaa gcaccggcgt
     8881 ttcctgcata tcttggcgga cttcgtcatc cgccgcctgc cgtccgcctt ctgccgcgcc
     8941 cccgtcatct tcttttgccc gtcgggaagg ttgcggcgca ataccgctgt tgtccagcgt
     9001 caagcccgga tcggttacca tacgttcctt catatagtat tcgccattgc tgctgaccac
     9061 accttcaggc attttcatcc ccttgccctg ctttcctttc aacgcaaaac gcatatagtc
     9121 cacccaaacc ggcaccgcaa tcgtaccgcc gtagccgaca cgccccatac tcttaggttt
     9181 gtcgaagccg atatatacgg cagtaaccac atcagggtta aaaccgacaa accacgcatc
     9241 cttattgtca ttggtcgtac ccgttttacc ggcaatatcc gttcttccca acgcagctgc
     9301 cccccttgcc gtaccgacac ggaccacatc ctgcataatc ttatacataa tataggcatt
     9361 gcgcggatcg attgcctgag gcgcattttg cccggccacc aaaggttgca tttgggcgcg
     9421 caacctgccg tctctgtcat aaatcttatc gattacgtgc gaagaaaccc tatatccgcc
     9481 gttcgcaaat acgctatatg cctccgccac tttcaacggc gttgtctcgc ccgtacctaa
     9541 agccatagac aggcttgccg gcagctcgga cgacctgaag ccgaaacgcc ggatatactg
     9601 ttgcgcgtaa ccgacaccga tagacatcaa aatacggatg gaaaccatat tcttggaagc
     9661 cgtcagagcc tgtctcaaag taatgtagcc ggaatatctg ccgtctgaat ttttaggtgt
     9721 ccaaaccgaa ccgttcggcc ctttccccgg cagggaaatc ggcgcatcgt taaccaccgt
     9781 ggacgcggtc atccccttag ataatgccgc cgaatagaca aacggcttaa aggtcgaacc
     9841 cggctgccgc attgcctgaa cggcacgatt gaatgttttg ctgtgaaaat cataaccgcc
     9901 gaccagcgcg cgcacagctc cggtttttgc atccagcgaa accaaagccc cctgcagcaa
     9961 cggctcttga accaccgccc aacgcccgcc gttgtttttg acacggatga ccgcgcccct
    10021 gcggatacgg tcctccccca ttttttcatt attgaccgcg cgggccgcaa agcccaaggc
    10081 gcgcctgtca agcgtaaccc gcctgccgcc gggcagctgt atgacgacat ttttcttttt
    10141 agtcacatcc aacacaacgg cgggaaccat tttatcgacg gtatagagtc ccgacagata
    10201 ctggctgaca gtctcctcga catcttcact cttactcaaa tcgatatagt tttccgcacc
    10261 gcggtagctg ctgccgcgat cgaaattccg tagagccttg cgcaatgcct cggttgccac
    10321 cttctgatga tcggcgcgga ccgtggtata aaccttaaaa ccctgcgtat aggcatcttc
    10381 accgtatttc tcatacagtt cctgacgcac catttccgcc acatataaag cactctgatc
    10441 gattttccga acaaaccgct cgtaatgcag ttcctcattc aacgcctgat cgcgctgttg
    10501 cacggtaatc atcttctcct cgagcatatt gttcaaaata tacttctggc gcaacttggc
    10561 acgttctgga ttaacaatcg gattataggc agacggagcc ttgggcagtc ccgcaagcat
    10621 ggcggcttcc gccaaagtca aatctcggac attcttattg aaatagattt gcgcggcaga
    10681 tgcaaaacca taggcgcgct gaccgaggta aatctgattg aaatacaact cgaggatttt
    10741 gtctttgctt aaagactgct cgattttata ggcaagcaac acctcattga atttgcgtgt
    10801 gaacgttttt tcactgctca aataaaaatt tttcgccacc tgctgcgtaa tcgtactcgc
    10861 acccgactgc acgctgccgg acacgacatt gccgacggca gcgcgggcaa caccccaaac
    10921 atccaccccc caatgccggt aaaagcgttt atcctcggcg gcgataaccg cattccgcaa
    10981 cacctctggg aaatcgccga tttttgtaaa ttcgcgccgc tgctccccat acataccgat
    11041 gacttcccca tccgccgaat aaatagtcaa cggcatttta ggctggtaat gctgcaaaga
    11101 atccaaagac ggcagtttcg gatacgttac caaaatagca atggcaacca aacccactcc
    11161 aaatacacaa aacccaaaaa ccaaaccaaa acaagtcgtt aaaatctttt taatcatagc
    11221 tgaataataa tttaccatta ttggtattaa ataaagtaaa atagcaaccg atttctacaa
    11281 agcacggttt caatgtgcaa agaacaagga atccattacg gataccgaaa cggttactca
    11341 ctgtacaaat aaagcaggaa ctttcatcat gcgcttgttt aaaagcttga aaaaccctaa
    11401 aaaaacagat gccaagctcc ctaaaaaatc ttcgggactc aataaccgcg cggcaatcgg
    11461 catcgatatc gaccagcatt ccatcaaaat ggtccaattg tcaggacgta gtttaaacca
    11521 aattcaattg gaaaaatacg tcattgccaa attaccaaag aatatcattc aaggcaataa
    11581 agtccaaaat tacgatcaac ttgttacata tttgcaacaa gcctatgcca aactgggtac
    11641 ttcgtgcaaa aacatcatcg cgtccgtccc gcaaaatctg gcaaccatcg aacaattgac
    11701 ctacacagac aaagatgcag aattagacct gcaggggttc gtggagtcct ccatctccga
    11761 agtcagctcg atatcgctcg aagaagccaa ttacgactat caggtcttgt cccaatcggc
    11821 cgccggcgaa gctgtgttgg ccgtcgcatc gagaaaggat gaaatcgaac ccctgattga
    11881 cgcattcaac gccgccggta tgaaattatc cgcgcttgat gtggacattt tcggacaata
    11941 caacgcctac gcgctatgga taaaccattt cgcccccgag cttgcggccg aaaaagtcgc
    12001 cattttcggc gtatatgccg cacagaccta cgccttggtc atccaagacg gaaaactcct
    12061 atacaaacag gaaacctccg tcagcgaaga acagctcaac caactcatcc agcgcaccta
    12121 tcaggtaaca gaagaaaaag cggaagaaat catcaactcc ccgcaaaaac cttccgatta
    12181 ccaagaaagc gtggcaaact atttcaacca gcagattacc caagaaatac aaagggtctt
    12241 gcagttttat tacaccacgc agaccgcaga cgatatgacc gacatcaagc atatcctgct
    12301 gaccggggaa gcggcgcgcc aggaaggcat cgcccaaacc gtcgcctcac aaaccaatgc
    12361 agatgtacaa tgcgtccatc ccgcgcgtta ttttgcggac aacctcaaaa cagacaaaca
    12421 acaattcgaa cttgatgcgc cgacactgac cagggcgttc ggtttggcgg tacggggatt
    12481 ataattatga acaatttaat caaaatcaac ctcctcccct acagggaaga gatgaacaag
    12541 cgcaaacagc agcagtttaa aacgctgatg tacggtgccg tgctgacggg cgttgccgcc
    12601 gttgcggcaa cctacctgtt tatcgacaat atgatcaata accagtcgga aagaaacacg
    12661 ctgctggaaa cctccatcgc acacttggat accgagctgt cggaaataca aaagctcaaa
    12721 caggaaaaag atgccttcct gattaagaaa aacaaaatcg aggagctcca gctcaaacgc
    12781 ctccaagccg caaaaatcct cgacagcctg aatgaggccg tccccggaag cacctacctg
    12841 acctcgctgg atgccgttac cgccgactct tatcggctca gcggcaggac atccagcgac
    12901 aaccgcgttg ccgccatgat gagggcgatg cccaataccg gcatattcaa gcaacccgaa
    12961 ttgttaagca tcaagaaaaa caattcgcat caagaattta cccttcaggc aacattacaa
    13021 cccatcgtaa aggcggccga atccaaagag aatccggctt cgggaaacgc acaggaggca
    13081 aactgaatgg cttctaaatc atctaaaacc aacttggatc tcaacaacct tcacctgctc
    13141 aaccttcctg ccaggctttt tatcgccctg ctggccgttg ccgccgtgct ggggctcggt
    13201 tatgccggat tgttcaaaag ccagatggaa tcccttgagg aatacgaagc aaaagaaacc
    13261 gaactgaaaa acacctacaa acagaaaagt atcgacgcgg ccagcctgaa caacctgagg
    13321 gacgaacttg cctcaatccg ctctgccttc gatatcatgt tgaaacagct gccgacagat
    13381 gcagaaattc ccaacctggt tcaagagctt catcaggcag gttcgagcaa cggcctgcgc
    13441 ttggacagcg ttatgcccca acctcccgta gatgacggcc ccatcaaaag attaccctat
    13501 tccatttcca ttaccggaaa ttacgaacag atcagccaat ttacccgcga tgtcggcagc
    13561 ctctcccgaa tcattaccct tgagtcgctg aaaatcgccc aatctccgga aaacggcggc
    13621 aatcctgacg gcaagagcag catcctgaac ctcagtgcca ttgccaccac ctaccaagca
    13681 aaatccgtag aagagcttgc cgcagaagcg gcacaaaatg ccgagcaaaa ataacttacg
    13741 ttagggaaac catgaaacac tatgccttac tcatcagctt tctggctctc tccgcgtgtt
    13801 cccaaggttc tgaggaccta aacgaatgga tggcacaaac gcgacgcgaa gccaaagcag
    13861 aaatcatacc tttccaagca cctaccctgc cggttgcgcc ggtatacagc ccgccgcagc
    13921 ttacagggcc gaacgcattc gacttccgcc gcatggaaac cgacaaaaaa ggggaaaatg
    13981 cccccgacac caagcgtatt aaagaaacgc tggaaaaatt cagtttggaa aatatgcgtt
    14041 atgtcggcat tttgaagtcc ggacagaaag tctccggctt catcgaggct gaaggttatg
    14101 tctacactgt cggtgtcggc aactatttgg gacaaaacta cggtagaatc gaaagcatta
    14161 ccgacgacag catcgtcctg aacgagctaa tagaagacag cacgggcaac tgggtttccc
    14221 gtaaagcaga actgctgttg aattcttccg acaaaaacac cgaacaagcg gcagcacctg
    14281 ccgcagaaca aaattaagaa gaggattact ccattatgaa taccaaactg acaaaaatca
    14341 tttccggtct ctttgtcgca accgccgcct ttcagacagc atcggcagga aacattacag
    14401 acatcaaagt ttcctccctg cccaacaaac agaaaatcgt caaagtcagc tttgacaaag
    14461 agattgtcaa cccgaccggc ttcgtaacct cctcaccggc ccgcatcgcc ttggactttg
    14521 aacaaaccgg catttccatg gatcaacagg tactcgaata tgccgatccc ctgttgagca
    14581 aaatcagtgc cgcacaaaac agcagccgtg cgcgtctggt tctgaatctg aacaaaccgg
    14641 gccaatacaa taccgaagta cgcgggaaca aagtttggat attcattaac gaatcggacg
    14701 ataccgtgtc cgcccccgca cgccccgccg taaaagccgc gcctgccgca ccggcaaaac
    14761 aacaggctgc cgcaccgtct accaagtccg cagtatccgt atccaaaccc tttaccccgg
    14821 caaaacaaca ggctgccgca ccgtttaccg agtccgtagt atccgtatcc gcaccgttca
    14881 gcccggcaaa acaacaggcg gcggcatcag caaaacaaca gacggcagca ccagcaaaac
    14941 aacaggcggc aacaccagca aaacaaacca atatcgattt ccgcaaagac ggcaaaaatg
    15001 ccggcattat cgaattggct gcattgggct ttgccgggca gcccgacatc agccaacagc
    15061 acgaccacat catcgttacg ctgaaaaacc ataccctgcc gaccacgctc caacgcagtt
    15121 tggatgtggc agactttaaa acaccggttc aaaaggttac gctgaaacgc ctcaataacg
    15181 acacccagct gattatcaca acagctggca actgggaact cgtcaacaaa tccgccgcgc
    15241 ccggatactt taccttccaa gtcctgccga aaaaacaaaa cctcgagtca ggcggcgtga
    15301 acaatgcgcc caaaaccttc acaggccgga aaatctccct tgacttccaa gatgtcgaaa
    15361 tccgcaccat cctgcagatt ttggcaaaag aatccgggat gaacattgtt gccagcgact
    15421 ccgtcaacgg caaaatgacc ctctccctca aagacgtacc ttgggatcag gctttggatt
    15481 tggttatgca ggcacgcaac ctcgatatgc gccagcaagg gaacatcgtc aacatcgcgc
    15541 cccgcgacga gctgcttgcc aaagacaaag ccttcttaca ggcggaaaaa gacattgccg
    15601 atctgggcgc gctgtattca caaaacttcc aattgaaata caaaaatgtg gaagaattcc
    15661 gcagcatcct gcgtttggac aatgccgaca caaccggaaa ccgcaatacg cttgtcagcg
    15721 gcaggggcag cgtgctgatc gatcccgcca ccaataccct gattgttacc gatacccgca
    15781 gcgtcatcga aaaattccgc aaactgattg acgaattgga cgtacccgcg caacaagtga
    15841 tgattgaggc gcgtatcgtc gaagcggcag acggcttctc gcgcgatttg ggcgttaaat
    15901 tcggcgcgac aggcaagaaa aagctgaaaa atgatacaag cgcattcggc tggggggtaa
    15961 actccggctt cggcggcgac gataaatggg gggccgaaac caaaatcaac ctgccgatta
    16021 ccgctgccgc aaacagcatt tcgctggtgc gcgcgatttc ctccggtgcc ttgaatttgg
    16081 aattgtccgc atccgaatcg ctttcaaaaa ccaaaacgct tgccaatccg cgcgtgctga
    16141 cccaaaaccg caaagaggcc aaaatcgaat ccggttacga aattcctttc accgtaacct
    16201 caatcgcgaa cggcggcagc agcacgaaca cggaactcaa aaaagccgtc ttggggctga
    16261 ccgttacgcc gaacatcacg cccgacggcc aaatcattat gaccgtcaaa atcaacaagg
    16321 actcgcctgc gcaatgtgcc tccggtaatc agacgatcct gtgtatttcg accaaaaacc
    16381 tgaatacgca ggctatggtt gaaaacggcg gcacattgat tgtcggcggt atttatgaag
    16441 aagacaacgg caatacgctg accaaagtcc ccctgttggg cgacatcccc gttatcggca
    16501 acctctttaa aacacgcggg aaaaaaaccg accgccgcga actgctgatt ttcattaccc
    16561 cgaggattat gggtacggcc ggcaacagcc tgcgctattg atgcgtcaaa ataagggcat
    16621 atgttttacg gcatatgccc tttctttatg ctttttgccg cgaccgaaat gccgtcattc
    16681 ccgcgcaggc agaaatccaa cttgtccggt tccggttgtt tttcgtctcg taacttttga
    16741 gccgtcattc ccgcgcaggc agaaatccag tccgttcagt ttcggtcatt tccgataaat
    16801 tcctgttgct tttcatttct ggattcccac ttttgtggga atgacggcgg agaggttttt
    16861 gttgtttcgg agaagtttct gcaaccctag aatctcgtta tttccacaaa aaacagaaac
    16921 ccgaaacagc aacttaaaac ctcgtcattc ccgcaaaagc gggaatccgg tccgttcagt
    16981 ttcggtcgtt tc
//
LOCUS       DAXIHS010000042        16467 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXIHS010000042 DAXIHS010000000
VERSION     DAXIHS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.042"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(236..1132)
                     /gene="argB"
                     /locus_tag="ACFOF2_001194"
     CDS             complement(236..1132)
                     /gene="argB"
                     /locus_tag="ACFOF2_001194"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC7107516.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1302..2432
                     /locus_tag="ACFOF2_001195"
     CDS             1302..2432
                     /locus_tag="ACFOF2_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC7107517.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRIAALEAQFKNALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2503..3354
                     /gene="lgt"
                     /locus_tag="ACFOF2_001196"
     CDS             2503..3354
                     /gene="lgt"
                     /locus_tag="ACFOF2_001196"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC7107518.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     LSMKKQH"
     gene            complement(3437..4099)
                     /locus_tag="ACFOF2_001197"
     CDS             complement(3437..4099)
                     /locus_tag="ACFOF2_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC7107519.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGTIRLQFT
                     EGELAELESELPYSLADIYRYWQAVINKPQTVRREGKKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            4133..4240
                     /locus_tag="ACFOF2_001198"
                     /pseudo
     CDS             4133..4240
                     /locus_tag="ACFOF2_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4299..6092)
                     /locus_tag="ACFOF2_001199"
     CDS             complement(4299..6092)
                     /locus_tag="ACFOF2_001199"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC7107520.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRSLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6338..7447
                     /gene="proB"
                     /locus_tag="ACFOF2_001200"
     CDS             6338..7447
                     /gene="proB"
                     /locus_tag="ACFOF2_001200"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC7107521.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGSALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7460..8722
                     /locus_tag="ACFOF2_001201"
     CDS             7460..8722
                     /locus_tag="ACFOF2_001201"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC7107522.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYVDKDADLEKAIRIVRNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(8947..11979)
                     /locus_tag="ACFOF2_001202"
     CDS             complement(8947..11979)
                     /locus_tag="ACFOF2_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC7107523.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAEFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGSGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            12144..12824
                     /locus_tag="ACFOF2_001203"
     CDS             12144..12824
                     /locus_tag="ACFOF2_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC7107524.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12853..13212
                     /locus_tag="ACFOF2_001204"
     CDS             12853..13212
                     /locus_tag="ACFOF2_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC7107525.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(13868..14404)
                     /locus_tag="ACFOF2_001205"
     CDS             complement(13868..14404)
                     /locus_tag="ACFOF2_001205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC7107526.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(14438..14794)
                     /gene="folB"
                     /locus_tag="ACFOF2_001206"
     CDS             complement(14438..14794)
                     /gene="folB"
                     /locus_tag="ACFOF2_001206"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC7107527.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            14850..15452
                     /gene="plsY"
                     /locus_tag="ACFOF2_001207"
     CDS             14850..15452
                     /gene="plsY"
                     /locus_tag="ACFOF2_001207"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC7107528.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGGSR"
     gene            15529..16374
                     /locus_tag="ACFOF2_001208"
     CDS             15529..16374
                     /locus_tag="ACFOF2_001208"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC7107529.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
BASE COUNT         3698 a         4743 c         4119 g         3907 t
ORIGIN      
        1 atcaagacaa ggcgaccaag ccgcagacag tacaaatagt acggaaccga ttcacttggt
       61 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
      121 tttgttaatc cactatattt tttcaggaat gacggtttgg aaattgcccg aaaccccaaa
      181 aacagaaacc ggacaaacgg gttttccgcc aaagccggca ttttccgact ttgcttcagg
      241 catcttcccc accgcccaaa atcatcgaac cgataccggc atcggtaaag atttccagca
      301 aaagcgcgtt gggcaccctg ccgtcgatga tgtgcgtggc tttcacgccg ttgacggcgg
      361 cttcgaccgc ggaagcgatt ttcggcagca taccgccata cagcgtgccg tcggcaatca
      421 gttcatcaat ccgtttcggc gtgagtttgg tcagcagatt gcccgttttg tccatcacac
      481 cggcgatatt cgtcatcatc aaaagttttt cggcgttcaa ttcttccgcc aatttgcctg
      541 ccaccaaatc ggcgttgatg ttgaacgctt cgcctttttc acctacgccg acgggggcga
      601 cgacgggaat gcagccacgt tctatcagcc ctttaaccaa accggtatcg atgctttcca
      661 ccgtaccgac ctgtccgatg tccacgccat tctgttcggg cgtatcaatc aaaagtttct
      721 tcgccttaat gaaatggtcg tcgcgtccgc ttacgccgac cgcgtgtccg ccatatgtgt
      781 taatcatcga cacgatttct ttattgacat gcccgcccaa caccatttcg acaatatcca
      841 tcgcctcttt gtcggtaacg cgcattcctt ggacaaactc accctttttg ccgacttttt
      901 caagcatcgc attgatctgc ggcccgccgc cgtgaacgat gacgggatga atgccgacca
      961 gcttcagcag cacgacatcg cgggcaaacc cttctttcaa ggcaggttcg gtcatcgcgt
     1021 tgccgccgta tttgatgacg gcgaccgaac cggaaaaccg gcggatgtaa ggcagcgctt
     1081 cggcaaggat acgcgccttg tcggcggcgg aaataatgtt ttcagactcc ataacggctc
     1141 cctattgtta acaatttcgg cttatgttaa cgccaaacgc cgtctgaaac aatcttcaga
     1201 cggcatccgg cagactttcg ggcaggttga agccttgcga tacgggcggt aaaatcgcgg
     1261 tttatccggt tcgagaaacc ggcgacagca aggactttcc catgattaaa atcagcaccc
     1321 gcttcgatgc cggctcggtc gtcgtcaaag acctgaccga tccttccaac atccgcctcg
     1381 ccctgcgtcc cgacaacgcc tccgattttg cacaatggtt ctacttccgg ctgcaaggcg
     1441 cggcatatca aaactgcatc atgcattttg aaaacgcggc agaagccgcc tacccgaaag
     1501 gctgggaagg ctatcaggcg tgtgcctcat acgaccgccg caactggttc cgcgtaccga
     1561 cttcctacga aaacggcgta ctgaccgtca accatacccc gctgtccaat agcgtgtact
     1621 acgcctattt cgaaccctat tccgaagaac agcacctcaa cctcctcggc gacgcgcaag
     1681 gcagcggcct gtgccgcatc gacgacttgg gcagcaccgt gcaagggcgc gacatcaatc
     1741 tgctgaccat aggcaatcag gtcgaaagcg atttgaaaat ctggattacc gcccgccagc
     1801 atccaggcga aaccatggcg gaatggtttg tcgaaggact gctcggcagg ctgctcgacc
     1861 atcaggatgc aaccgcgcgc accctgctcg atcgcgccac gttctatatc gtccccaata
     1921 tgaaccccga cggttcggca ctgggcaacc ttcgtaccaa cgccgccggt gcgaacctca
     1981 accgcgaatg ggaaaatccg actttggaaa aaagccccga agtgttcttc gtgcgtgaaa
     2041 gaatgctgga aaccggcgtg gatttgtttt tggatatcca cggcgacgaa gggttgccct
     2101 ttgtctttgt cgcaggtacg gaaggtgtgc cgaactacaa tccgcgcatc gccgcgttgg
     2161 aagcgcagtt taaaaatgcc cttttaaacg ccagccccga tttccaagac gaatacggtt
     2221 acgaaaaaga tgcgcccggt caggcaaacg tgaccttggc aacaaactgg gtcggcaacc
     2281 gtttcaactg ccttgcctat acgttggaaa tgccgttcaa agacaatgcc aacctgcccg
     2341 acgacgactt cgggtggaac ggtcaacgtt cgttgcgcct gggcgaagcc gcgttgtccg
     2401 cgatcctcaa cgtcatcggc gatttgcgct gaaaccctta ttgcagaaat gccgtctgaa
     2461 gcatcatgcc tttcagacgg catttgaaaa cgaaagcctg ttatgattat ccatccccaa
     2521 ttcgatcccg tcctcatcag tatcggcccg cttgccgtcc gctggtatgc cctaagctac
     2581 atcctcggat ttattctttt tacctttctc ggcagaaggc gcatcgcgca aggcttgtcc
     2641 gtttttacca aagaatcgct tgacgacttc ctcacttggg gcattttggg cgtaattttg
     2701 ggtgggcgtt tgggttacgt cctgttttac aagttttccg actacctcgc ccatccgctt
     2761 gatattttca aggtatggga aggcggaatg tcgttccacg gcggcttttt gggtgtagtt
     2821 attgccatat ggttgttcgg ccgcaaacac ggcatcggct tcctcaaact gatggacacg
     2881 gtcgcaccgc tcgttccgct gggtctcgct tcgggacgta tcggcaactt catcaacggc
     2941 gaactttggg gacgcgttac cgacatcaac gcattttggg caatgggctt cccgcaggcg
     3001 cgttacgaag atgccgaagc cgccgcgcac aatccgcttt gggcagaatg gctgcaacaa
     3061 tacggtatgc tgccgcgtca tccctcgcag ctttatcagt ttgcacttga aggcatctgc
     3121 ctgttcgccg tcgtttggct gttctctaaa aaacagcggc cgaccggaca agtcgcctcg
     3181 ctcttcctcg gcggctacgg catattccgc ttcattgccg aattcgcacg ccaacccgac
     3241 gactatctcg ggctgctgac cttggggctg tcgatggggc aatggttgag cgtccctatg
     3301 attgttttgg gtatcgtcgg ctttgtccgg ctcagcatga aaaaacagca ctgaccgcct
     3361 gtgaaaaata ccgtctgaaa catggaaatc cggtttcaga cggcattttt acgtttggtt
     3421 catggaaaca aacgcttcaa ttcttaccgc aacacgcctt gtattttctg ccgctgccgc
     3481 acggacaggg atcgttcctg cctgtttttt tgccttccct gcggacggtt tgcggtttgt
     3541 tgatgactgc ctgccagtag cggtagatat ccgccaatga ataaggcaac tcggattcca
     3601 gttccgccag ctcgccttct gtgaattgca gacggatagt gccgttttcc tcttcgtcgt
     3661 aaataccgcc caatgccatg atgggataaa acaactcttc aaacgcttca tcatcgacgg
     3721 cttcaaacca atcggtcggc acaatatcca aaccgtaaag ataagcattg caccatgtgt
     3781 aaaaatcgct gccgccgtct tcgttttcat acagccacaa atcgggcagt tttttatccg
     3841 acatcgcggc ggttgtttcc atcgccattg ccaaaaccag ccgttcgatt tcggaacgtt
     3901 cggcggcggt aaattgcgat tcgtcgccca acacttcggg cagccagtcg agcggtgtca
     3961 atttgtccgg cccgctcaac agcgccgtca taaaaccttg aacctcgtcg caacgcatcg
     4021 tgttgccttg ttcgcttttg gcatccaaca attcgctcaa ccgccgtttg gatgcttcgg
     4081 taaattttcg ggaatccatc attttccttt tcaaatgggt tttgcgccct attatagtgg
     4141 attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
     4201 cgaggcaacg ccgtactggt ttaaatttaa tccactatat cgccgcaatg ccgtctgaag
     4261 cctttttatg cttcagacgg catcgtctgt tttcacgctc aaatcgtacc gctgccttgc
     4321 gctttcggtt tggcggcact aaattccagc ttgctcaaag cggaaaggta ggctttggcg
     4381 gtggcgacca aaacgtcggt atccgcgccc tgaccgttga cgacgcggtt gccgcgcgcc
     4441 agacggacgc tggtttcgcc ctggctttcc gtaccttgcg tgacggcgtt gacggaataa
     4501 atctgcaaag ccgcgccgct ttgcgccacg ctttcaatcg ctttgaaaat cgcatccacg
     4561 gggccggagc ctgtggcgga agcgcgtttt tcttcgcctt tgatgctgaa cacgatgtcg
     4621 gcgcgcggct cttcgccggt ttcggtgctg attttttggg agatgaattt gtagctctcg
     4681 gcattcatgc tgcccatttc gtcggatacc agtgcgtgca ggtcttcatc gaagatttcg
     4741 cgttttttgt cggcgagttc tttgaagcgg gcaaacgcgg cgttcagtgc ctcttcgctt
     4801 tccaactcga tgcccaaatc cgccagcttg gttttgaagg cgttgcggcc ggacaatttg
     4861 cccaagctca aacggtttgt tgcccagccg accgattcgg cggacataat ctcgtaagtt
     4921 tcgcggtgtt tcagcacccc atcctgatgg atgcccgatt catgcgaaaa ggcattggca
     4981 ccgacaatgg ctttgttggg ctgcacggga tagcccgtaa tggtggacac cagtttggac
     5041 gaaggcacga tttgcgtggt atcgatgccg gtttccaagc cgaacaagtc gtggcgcact
     5101 ttcaacgcca tcacgatttc ttcaaccgaa gcattgcctg cacgttcgcc caagccgttg
     5161 acagtacatt ccacctgacg cgcgccgcct tttaatgcgg caagcgaatt ggcaaccgcc
     5221 aagcccaaat cgttgtggca gtgtgccgac caaacgactt tgccgccgtt gggcgttttg
     5281 gcaatcagtt cgcggaaaaa ttcttcggtt ttgtacggga ttgaatagcc gacggtatcg
     5341 ggaatattga tggtggtcgc gcccgcttca atcaccgcgc cgcagatttc ggcaaggaaa
     5401 tcgatttccg aacgcaacgc gtcttcgcag gaaaattcca catcgtcggt gtattcacga
     5461 gcgattttca ccgctttgac cgccgcctca atcacctgct tcggcttcat cttcaatttg
     5521 tactccatat ggatggggct ggtggcgatg aaggtgtgga tgcgtttttt cggcgcgggc
     5581 gcaacggcct cacccgcctg acggatgtcc cgctcgatgg cgcgggacaa tgaacagacc
     5641 gttgatttgg taatggtttt cgcaatcgca ttgaccgcct cgaaatcgcc cgggctggca
     5701 gcggcaaaac ccgcttcgat gatgtccaca cccaattttt ccagctggcg ggcgacgcgg
     5761 attttttcct ctttggtcat agcggcgccg ggcgactgct cgccgtcgcg cagggtggta
     5821 tcaaaaataa ttacgcggtt ggtctgtgtc atttctgttc tctccagaga ttcgtttttt
     5881 tgtaaaaaag cattcaaatc cagcgacctg ccttgcgctt cagccaacgc ggtcagcttt
     5941 tgcagttgcg ccaggggcag cgacccgcgc gtgcgccatt tatagatggc ggcggtcgtc
     6001 gcggcatttt cgggatcgtg ctgtttcaac gcttcggcaa gcgcgttcac gccgccgaaa
     6061 taagcaacca agcggtctat atctaattgc atggtagtcc ttgtctaaaa taaatcagcc
     6121 tacagcagaa atatggacag gattatacgc attttggaca aaacggcaat ataaatttga
     6181 aaaatctcaa taagccgcca tagattttat taattttata cattttgtct attttatcgc
     6241 agacgcggcg tgattgttga caattgcggc agaacagggc aaattcggcg gcacaaggcg
     6301 gcaaacgaga caggacaaac caacaaggac ggaaacaatg aaatacaaaa gaatcgtatt
     6361 taaagtcggc acatcttcga ttacccattc ggacggcagt ctctcgcgcg gcaaaatcca
     6421 aaccatcacc cgccagcttg ccgcattgca tcatgcggga cacgagctgg tcttggtgtc
     6481 ttccggcgcg gttgcggcag ggttcggtgc gctgggtttc aaaaaacgtc cggtcaaaat
     6541 cgccgacaaa caggcttccg ccgccgtcgg gcaggggctg ctgatggaag aatatacggc
     6601 aaacctgtct tcagacggca tcgtgtccgc acaaatcctg ctcagccgcg ccgactttgc
     6661 cgacaaacgc cgctaccaaa atgccggcag cgcactttcc gtgctgctgc aacgccgcgc
     6721 cgtccccatc atcaatgaaa acgatacggt ttcggttgag gaattgaaaa tcggcgacaa
     6781 cgacacattg agtgcgcaag tggcggcgat gatacaggca gacctcttgg tgctgctgac
     6841 cgacatagac ggtctttaca cgggcaaccc gaacagcaat cccgatgccg tacggctgga
     6901 caaaatcgaa cacatcaacc atgaaatcat cgaaatggcg ggcggctcgg gttcggcaaa
     6961 cggcacaggc ggtatgctga ctaaaatcaa agcggcgacg attgcgaccg agtccggcgt
     7021 accggtctat atctgttcct cgctcaaacc cgatgcactt gccgaagcgg cagataatca
     7081 ggcggacggc tcgtttttcg tcccccgtgc caaaggtttg cggacgcaga agcaatggct
     7141 ggcgttctat tccgaaagcc ggggcggcgt ttatgtggac gaaggtgcgg aacacgcttt
     7201 gtccgaacag ggaaaaagcc tgctgatgtc gggcattgcc ggaatcgaag ggcatttttc
     7261 ccgtatggac accgtaaccg tgtacagcaa ggcaaccaaa cagcccctgg gcaaagggcg
     7321 cgtcctgttc ggttctgccg ccgccgaaga cctgctcaaa tcgcgtaagg cgaaaggcgt
     7381 gttcatccat cgggacgact ggatttccat cacgcccgaa atacgcctgc ttctgaccga
     7441 attttagaaa aggaaaccca tgtcaaacac acaaaaacag cttgcccttg ccaaagcggc
     7501 aaaaaaatcc gtcaacacgg cggatacgga agaaaaaaac cgcgcgctgt ttgctatggc
     7561 ggacagcttg gaagcggcgg cggcagatat tttggcggca aaccgtcaag acttggaagc
     7621 cgcggcaggc aacattcccg aaagcatgac cgaccgcctt ttgttggacg gcaaacgcat
     7681 ttgcgcgatg gcggacggca tcagggcggt tgccgcgctg cccgaccccg tgggcgaaat
     7741 actggaaacc tcgactttgc ccaacggctt ggaaatcgtc aaaaagcgcg tggcgatggg
     7801 cgttatcggc attatttacg aaagccgccc gaacgttact tccgatgcgg cggctttggc
     7861 actgaaaagc ggcagcgcgg tcgtactccg cagcggcaaa gatgcattcc aatccgcacg
     7921 cgccatcgtt gccgccctga aaacggggtt ggcacaaacc cgcatcgacc ccgacgcgct
     7981 ccagttgatt gaagacaccg ggcgcgaggg cagttacgaa atgatgaggg cgaaagatta
     8041 tctagacctg ctgattccgc gcggcggggc ggggctgata cgggcggtgg ttgaaaatgc
     8101 cgtcgtgccg gtcattgaaa cgggaacggg catcgtccac atctacgtcg ataaggatgc
     8161 ggacttagaa aaggcaatcc gcattgtccg caacgccaaa accagccgtc cgtccgtgtg
     8221 caactcgatg gaagtgctgc tggtacatga agacattgct gccgacttcc tgcccaagct
     8281 cgaacggctg ttggttcgcg accgtataga agccggactg ccgcccgtcc gcttccgttt
     8341 ggatccgcag gcggcgcggc atatcggcgg cgaagcggcg ggtgcagacg atttcgatac
     8401 cgagttttta gactacatcc tcgccgtgaa aaccgtcgct tcggtcgaag aggcggtcgg
     8461 gcacatcgaa acccacggca cgcaccattc agacggcatc gttaccgaaa accgccacgc
     8521 tgcggactat ttcacgaccc atatcgattc tgccgccgtg tatgtcaacg cgtccacgcg
     8581 ctttaccgac ggtggcgaat tcggcttggg ttgcgaaatg ggcatctcca cgcaaaaact
     8641 ccacgcccga ggcccgatgg gcttgaaaga gctaacgagt tataaataca tcgtacaagg
     8701 cacgggacag gtcagggaat aatccgaatt caaacgtctg atatagtgga ttaactttaa
     8761 accggtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca
     8821 ccaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8881 tttgttaatc cactataaaa atgccgtctg aaacagtctt cagacggcat ttccatttgc
     8941 aaaaaatcaa gcattgtcca aggggacgag aatcgtacgg ttgccgttgt gttccggtgc
     9001 ggacacaatg ccttccgcct ccatctggtc aatcagacgc gcggcgcggt tgtagccgat
     9061 gcgcaaggcg cgctgtacgc ccgaaatact ggctttgcgc gttttcagga caacggatac
     9121 ggcttcatca tacatcggat cggtttcgtc gtcgccgctg cgcccgatgc cgggcagctc
     9181 ttcgctgccg ctgccgctca aaatatcgtc aacatagtcc ggctcgccaa actgtttcaa
     9241 atattcgacc acgcggtgca cctcttcatc cgaggcaaac gcgccgtgaa cgcgctgcgg
     9301 ataggcagta cccggcggca ggaacagcat atcgccctga ccgagcaggt tttccgcgcc
     9361 catttggtcg agaatcgtgc ggctgtcgat tttgctggac acttggaacg cgatacgcgt
     9421 cgggatgttc gccttaatca gacccgtgat gacatcgacg ctggggcgtt gtgtggcaag
     9481 aatcaaatgg atgcctgccg cgcgggcttt ttgtgcgagg cgcgcaatca gttcctcgat
     9541 tttcttgcct gccgtcatca tcaaatcggc aaactcatcg accacgacca cgataaacgg
     9601 cagtttttcc aaaggttcgg gatcgtcggg cgtgaggctg aacggattgc cgattttttc
     9661 tccccttgct gcggcttcgg cgattttttg attgaagccc gcaagattac gcacacccat
     9721 aaagctcatc aggcggtagc gtttttccat ttcgttaaca caccagttca gcgcgtttgc
     9781 cgccagcttc atatcggtaa cgacaggggc gagcaggtgc gggatgcctt cgtaaatgct
     9841 caattccagc attttcggat cgatcataat catacgcacg tcttccggcg cggctttgaa
     9901 aagcatagac agaatcatcg cgttgacacc caccgatttg cccgaaccgg tcgtgccggc
     9961 aaccaacaaa tgcggcgctt ttcccaagtc ggttacgacg ggctgtccgg tgatgtcctg
    10021 accgagcgcg agcgtcagct tggatttgga ttcggcaaac tcgggcgaat tgaagatttc
    10081 gctcaggcgt atcatttggc gtttcgggtt cggaagttcc aaacccatac aggttttgcc
    10141 ggggatggtt tcgacaacgc ggatggaagc cacgccgagc gaacgcgcca agtctttttc
    10201 cagattcaga acggaattgc cgcgcacgcc gacatcgggt tcgatttcat aacgcgtaat
    10261 cacggggccg gaataagaat cgacaacctt gaccttgact ttgaactccg ccaatttttc
    10321 ttcgatggtg atgctgtttt ccaacagttc ttcttcggtt tgcgtcgcct cgggattgaa
    10381 cagcggcggc agaagcaggt cggttgtcgg caggtgttcg gataccgcac cggtctcttc
    10441 cgattggaac gccccttcat ccgcttccgt atccgatacc cggcaggacg ggcgttcaga
    10501 cggcacattt tcaaacggac aaaccgcctg actgtcatgc ccgaacgctt cggtttccga
    10561 cagatattgc ccgcttgacc gctctgccgc accttcactg ccgtcatctg caatagcggc
    10621 ggtttcctcc tgccaacctc cattcaaaac atcatcggca agatggtcgg tttcggcaat
    10681 gcggctgcgt tgcgcctgct cgaattctgc cggcggttca taggtacggt tgtagatttc
    10741 cgatacggga ggcggcggcg gaatatcgat tgcgggcatg ggaactttcg gcacttcggg
    10801 tggttcgacc acccaagcat cgggcgcagc aggttcttca atatagatat cggcagcttc
    10861 atcataaacc ggaccgccgt atcccacaga cgaaacggtt tccgtgaaaa cattttcggt
    10921 ttcagacggc atttcgacat cccgtttccc gacaaccgtc cgactttccg gaatcaggcg
    10981 cgaaatccgc gcctccgcag aaaccgttgc cttgttcggt tctttcaaat tgacggaaac
    11041 gcggcggcag gcatcgcgga cggctgtgcc gtctgaaacg gactgtcctt gggaaacatc
    11101 cggggatttt gcctccgcct gccctgcatg acggcggaac ggcgcattcc gtgcattttc
    11161 ggctttgact gcagaaaagg cagacggatg ctgcttctca aaatacggct caaatccgta
    11221 atccgcagac tcggaaaacg cttctttgtc cgcatcgaac atacgggaat acgtgcgttg
    11281 aagcacaggg tcgtccaaac cgataatctg cagcccttcc atcggggtat cggacacgcg
    11341 gactttgggg accgctgttt cgtttttctc aaatgcatcg atatagcgtt ttttcgtttc
    11401 ccttaaagcc gcgccgttta aggcacgcgt tgcttcttcc aaagtgattt ccttaaaaac
    11461 cggacggacg ggcgaaattt cgctttcaga cggcatcaac ccttcagcaa tactccggtc
    11521 gaatgggatg cggcggttgt cgattacggc agttgcaatg tcttccgtat cggcagcctc
    11581 ttcctccgca gcttctgctg cttccgtttc cgcttcttcc gtcccgtttc cactgtctga
    11641 atatccgtcg gatgcgttcg gaacatccgc acggtttgca gaagcaggcg gaacaggcct
    11701 atccggttcg gattcgtgcg ccagatagtt tcggcagaaa cggacgacac cgacgaacag
    11761 ccaatacact gccgttttga cggcatggaa aagcatcagg gcaagttcgg gaaaatcggg
    11821 cataccgtct ttgatttcag gcagctccgc ctgtttttcc cctttctttt cctgccacgc
    11881 agaaacctcg cgcatccact cgcgttcgga ttgtgcgcgg acaaaaaaca agccggcaag
    11941 cgcaagcaac aaaataacga tcataactat ccaaaacata tgccgtttcc cgcaagacaa
    12001 acgattaaac gggtttattt taacgtgttt ccatcccaaa aaaaacaacc ggtcggcaat
    12061 tctgccgaaa ccgattcggc tttcggtttg cccccaccga cagtataata gccgtccctt
    12121 cccttaatct ttgcggcatt gccatgattt tccaaacagt ctggttttca gatgtggtac
    12181 tgtcggtttc gtggattgtc cttatcctga tactggcagc ttccgctccg tcggcattcc
    12241 gctcgcttgc ccgctaccgt tctgcccttc ccctgtgtac cgtaattttc tccgcggcat
    12301 ggtgtctgaa cgcctctgcc ggcggcggac agcttgcaca gatgaactac cacctgctcg
    12361 ccgtcaatct agttgctctg atggtgggca cttccgccgc cttttggctt gccgcgctgc
    12421 tgatgctgcc ctactgcctg ctgtttgccg gttctgtcgg cgcatatccg ccgaacgctt
    12481 tggtgctgat tctgcccgcg ctggccgtca accgcctgtc gcgtatgctg gtcaaccgcc
    12541 tgccacccaa tattttcatc tttattttcg tcaacggctt tctcgcttcc gccgccggca
    12601 ttctgctgac cgggctggtg ctgaccggta ttttggatgc cgcaaacgct tttccgtccg
    12661 aaatattgtg gacgaccgcc ctgcccgtct ttattctgct ggcgtgggcg gaagcttttc
    12721 tcagcggcat ttcaactgcc atatttgttg cactgaagcc ccattggatc aacactttcg
    12781 acgacaaccg ctatctgaag tccgaccgcg gcatatggcg gtagccttca gacggcatag
    12841 acaggcaaaa ccatgctttc caacatcatc cccctttcca tcggtgcggc actcggtgca
    12901 acggcgcgtt ggctgctcaa cctcgccgtt cctgccgcca tgtctcccgc cacgggcaac
    12961 ctgtttgcca actggacggg cgcgctgctc atcggcatct ttgccgaaac catcaatcac
    13021 ccgcaatgga agctgctgct gattaccggt tttctcggca gcctgaccac gctttccgga
    13081 ttttcactgg aaaccgtaac cctgctccaa tcgaatcgtc ctgcttcagc acttgccaat
    13141 attttcctac acacggcagg ttcgctgctg ctgacttggc tcgggctgaa gataggggcg
    13201 gcagtcaaat aaccgcaaca aattgcctcc ctttgttttg acataatcta taaattagct
    13261 tcgcttgttt aaatactggc tgtgccaatt atagtggatt aacaaaaatc aggacaaggc
    13321 gacgaagccg cagacagtac agatagtacg gaaccgattc acttggtgct tcagcacctt
    13381 agagaatcgt tctcttggag ctaaggcgag gcaacgccat actggttttt gttaatccac
    13441 tataccgttc tcaaattcaa attgggcttt taggtctata acaaagaaaa agccttggca
    13501 ggataaacct accaaggctt tttattaaaa aaaacgctct attactgtgt ctaaaaatgc
    13561 aaaaaaagta accatcataa cccttttgcc aaaatcaata tcatcttccg atgaatgaat
    13621 tgacggtata taagaaagaa ttaataagct cataaaccgg taagaaaaat cagtaacaaa
    13681 gattttcatt attaaccatt cttaaaaatg atcacgttcg tttgggtaat tattataccc
    13741 ttttccctct tattactaaa gcacgaatga ctttaaagga cgcgcccaga gacaagccga
    13801 aaaggcatat ctcatccgcc aaacaaaatg cccgccaatc tactccggag ggcagttcaa
    13861 caccctgtca atccttcatc agccagtatt gcaaaccgat taatgtcttg ccgtctttaa
    13921 tttcatcgtt tgccaatgcc tgacggactt cttctttcga catcaatacg gtttccgtaa
    13981 tctcgtcttc gtcattggca agcgtactgc ccaaacgcac gccttccgcc tcgaacagat
    14041 acattttttc gttgcaaaaa ccgaccgccg tataaaaact gtaaagcagg cgtacgctgt
    14101 cggtggtata aggcgtttct tcagccagtt ctcgtaaagc gcatgctgcc atatcctcgc
    14161 ccgccacatc cagcttgccc gcaggaagtt ccaatgtcgc ctgatttgcc gcataccgcc
    14221 actgccgtac caaaacgacc ttttcctcat ccgttaccgc caacacgcac gccgcgcccg
    14281 gatgccggat gacgatacgc tgcccttcat tgccgttggg caacctgacc ttatccctgc
    14341 tgatgctgac gaaaccgccc tcgtaaatgg tttcgccgcc taattttact tccctcaaat
    14401 ccacttcata tctcctttcc gtttcctatt ctgccgttca atcttcacgc ttgccgcgct
    14461 cgatttccac gccaacctcg cgcacgcctt ccaaaatacc cggcttgacg attttcacgc
    14521 gcacccacac cgcgccgaaa tatcccaaaa ccaaatcggc aatatattcc gccaacgctt
    14581 ccaaaagcag gaaatcctgt tctttcagat gtcggcgcag cgtttcgcat acctcggcat
    14641 aatgcaccgt attggcaata tcgtcgtccg aacccgcttt ctcgggaacg ccgatgtcca
    14701 aatcgacaat cagggtctgc aaccgttcgc gctcccagcc gtacacgccg ataagcgtat
    14761 ccgccttgat gccgtgcaaa aagattttat ccataatccg ccccgttttt tgctaaaata
    14821 accgcaccat tctaagtaaa gtaccgcaaa tgttcaacat accggctgtt gctgtttcct
    14881 atctgatcgg ctcactttct ttcgccgtca tcgtgtccaa gtattacggc atggacgacc
    14941 cgcgcaccta cggatcgggc aatccggggg caaccaatgt tttacgcagc ggcaaaaaaa
    15001 aggcggccgt gctgacgctc ttgggcgatg ccgccaaagg tttggttgcc gttttgcttg
    15061 cacgcgtgct tcaagaaccg ctcggtttat ccgacagcgc aatcgctgcc gtcgcactcg
    15121 ccgcgctggt cggacatatg tggccggtgt ttttcggatt taaaggcggc aaaggcgtgg
    15181 caacggcatt gggcgtgctt ctggcactct ctcccgcaac tgccttggtc tgcgcgttga
    15241 tttggcttgt gatggcattc ggcttcaagg tgtcctccct tgccgcatta accgccacaa
    15301 tcgccgcccc ccttgccgca ctgtttttta tgccgcatac ttcttggatt ttcgcaaccc
    15361 tcgcaatcgc catattggtg ttgctccgcc ataagagcaa catcctcaac ctgattaaag
    15421 gcaaagaaag caaaatcggc ggcagccgct gatttcccat ccatccctgt ttatgccgtc
    15481 tgaaccccgc ttcagacggc atccgaatat cccgccgttt taccgactat gctcagttac
    15541 agacacgcat tccacgccgg caaccacgcc gatatgctca aacacttcac cttgttttta
    15601 gtgctgcaat atttcaaccg caaagacaag ccctactggt acatcgacac gcacggcggc
    15661 gcgggtgtgt acaatctcga aggcagcgag gcacaaaaag tcggcgaata ccggcaaggc
    15721 atcgccctac ttcgacaagc aaaaaacctg ccaaccgagt taaacggctt tatcgcgcac
    15781 atacaaaaaa tcctgccctc gcccgaactt tactgcggtt ctccgtggct ggcgcaatcg
    15841 ctgacccgcg tcggcgacaa attacgcctg tttgagctgc accccaccga ctttgtccat
    15901 ctgcaaaaca atatgggcga agcaggattg ggcaaacgcg gacaagtgtt gcgcgaagac
    15961 ggttacaaag ggctgatttc cctgctgccg cccccgccgc gccgcgccac cgtcctcatc
    16021 gacccgccct atgaggaaaa acaggactac cggcgtgtaa cggaaacgct gaaagccgct
    16081 ttaaaacggt ttgagtccgg ctgttatctc atttggtatc cctgcctcag ccgcgaagaa
    16141 agccgcaagc tgcccgaaga attgaaaaaa ctcctgcccg acaactacct tcacgccgaa
    16201 cttcacgtcc acgcgccgaa agccgacggt ttcggtatgc acggcagcgg aatgttcgtc
    16261 atcaatccgc cctatctgtt ggcggaacaa ttggcggcaa acctccccgc cctgacgcgg
    16321 ctgctggcgc aagacgaagg cgcgcgctac cttttggaca gcaaaatccg ctgaccccga
    16381 tgccgtctga aacccttcag acggcatctc ccatcaaaca aaaccggacg atataccggc
    16441 agccggcact tttatagtgg attaaca
//
LOCUS       DAXIHS010000043        16144 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXIHS010000043 DAXIHS010000000
VERSION     DAXIHS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.043"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            6..81
                     /locus_tag="ACFOF2_001209"
     tRNA            6..81
                     /locus_tag="ACFOF2_001209"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="ACFOF2_001210"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="ACFOF2_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC7107530.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="ACFOF2_001211"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="ACFOF2_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC7107531.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="ACFOF2_001212"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="ACFOF2_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC7107532.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="ACFOF2_001213"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="ACFOF2_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC7107533.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="ACFOF2_001214"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="ACFOF2_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC7107534.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="ACFOF2_001215"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="ACFOF2_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC7107535.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3592..7770
                     /gene="rpoB"
                     /locus_tag="ACFOF2_001216"
     CDS             3592..7770
                     /gene="rpoB"
                     /locus_tag="ACFOF2_001216"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC7107536.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDKDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7927..12102
                     /gene="rpoC"
                     /locus_tag="ACFOF2_001217"
     CDS             7927..12102
                     /gene="rpoC"
                     /locus_tag="ACFOF2_001217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC7107537.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVNQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGAAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12394..12651
                     /locus_tag="ACFOF2_001218"
     CDS             12394..12651
                     /locus_tag="ACFOF2_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107538.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            12648..12797
                     /locus_tag="ACFOF2_001219"
     CDS             12648..12797
                     /locus_tag="ACFOF2_001219"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC7107539.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            12976..13347
                     /gene="rpsL"
                     /locus_tag="ACFOF2_001220"
     CDS             12976..13347
                     /gene="rpsL"
                     /locus_tag="ACFOF2_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC7107540.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13465..13935
                     /gene="rpsG"
                     /locus_tag="ACFOF2_001221"
     CDS             13465..13935
                     /gene="rpsG"
                     /locus_tag="ACFOF2_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC7107541.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13954..16059
                     /gene="fusA"
                     /locus_tag="ACFOF2_001222"
     CDS             13954..16059
                     /gene="fusA"
                     /locus_tag="ACFOF2_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC7107542.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4297 a         3579 c         4176 g         4092 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agatcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct
     1741 gcctaaaggc accggtaaga caacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcaattgggt accatcttgg gccctcgtgg tctgatgcca aaccctaaag taggtacggt
     1981 tacccctaat gttgccgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aagcattaag tattgtttac
     3481 atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca gattgatgcg
     3541 tgccgttgct ttcagacggc ctattattga aaattacttt tcggagtgta tatgagctat
     3601 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3661 gttcctttcc tgctggcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3721 gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat tttcccgatt
     3781 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3841 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     3901 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     3961 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4021 ggcacagagc gtgtgattgt ctcccagttg caccgttcgc ccggtgtatt cttcgagcat
     4081 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4141 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4201 cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa caatgagcaa
     4261 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4321 gatttggtcg cagaccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4381 ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg tgatattacc
     4441 aatgcaggcc tgacccgttt ggatgtagaa ccggaaagcc tgctgggcaa agcattggct
     4501 gccgatctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4561 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttatatc
     4621 aatgagctgg atcagggtgc ttatatctcc aataccttgc gtacggatga gactgccggc
     4681 cggcaggcgg ctcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccaccgaa
     4741 gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatctgtcc
     4801 cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4861 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgacaaaggc
     4921 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     4981 aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt acgttcggta
     5041 ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg tgccgtaaaa
     5101 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5161 cctgtttctg ccgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5221 cagaccaacc ccttgtctga agtaacccat aaacgccgtg tatctgcatt gggtccgggc
     5281 ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac ccactacggt
     5341 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcattgtcc
     5401 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5461 ggcaaagtaa ctgaagaaat cgactacttg tctgccatcg aagaaggccg ctatgtgatt
     5521 gcacaggcga atgccgattt ggacaaagac ggcaatctga ttggcgactt ggttacttgt
     5581 cgtgaaaaag gcgaaaccat tatggcaacg cctgaccgcg ttcaatatat ggacgtggca
     5641 accggtcaag tggtatccgt tgcagcatcc ctgattccgt tcttggaaca cgatgacgcg
     5701 aaccgcgcat tgatgggtgc gaatatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5761 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5821 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgtgttgt gattcgtgtc
     5881 catgacgacg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt ggttaaattt
     5941 acccgttcca accagtctac caatatcaat cagcgtcctg ccgtcaaagc cggcgatgtt
     6001 ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga attggctttg
     6061 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6121 ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat tgaggaattg
     6181 aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga tattccgaac
     6241 ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat cggtgcggaa
     6301 gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac ccagctgacg
     6361 ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt aaaagatact
     6421 tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt cttcactcgt
     6481 gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgagtt gaaacgctac
     6541 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgag
     6601 cgtatgattg tcggtcagaa agccaacggc ggcccgatga agctggccaa aggcagtgaa
     6661 atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6721 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6781 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6841 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     6901 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     6961 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7021 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7081 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7141 gagttcttga acaaactcta caacggcagc ggcaagaaag aagatttgga tgccctgact
     7201 gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt cgcctctcct
     7261 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgt tgaacttggc ttatccgagc
     7321 gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat cacgctgtat
     7381 gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat gcactatctg
     7441 aaactgcacc acttggttga cgaaaaaatg cacgcgcgtt ctaccggtcc gtacagtctg
     7501 gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt cggtgagatg
     7561 gaggtttggg cactggaagc atacggcgca gcctacacgc tgcaagagat gctgactgtg
     7621 aagtctgacg acgtgaacgg ccgtaccaaa atgtacgaaa acatcgtcaa aggcgaacac
     7681 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat tcgctcactg
     7741 ggcttggata tcgatttgga acgttactaa acaaaagttt tcagacggcc tttcagggtc
     7801 gtctgaaaaa gtggtttcag aataagaatg aagcaatcgg catttaggcc gtctgaaatc
     7861 aaaagtaccg tttccccata tcgaaaatcc gccatgcggt aaaaatactt ccttcaagga
     7921 gcaaaaatga atttgttgaa cttatttaat ccgttgcaaa ctgccggcat ggaagaagag
     7981 tttgatgcca ttaaaatcgg tattgcctct cccgaaacca tccgctcatg gtcttatggc
     8041 gaagttaaaa aacctgaaac catcaactac cgtacgttca aacctgagcg tgacggtttg
     8101 ttctgtgcca aaatctttgg cccggtcaaa gactacgaat gcttgtgcgg aaaatacaaa
     8161 cgcttgaaat ttaaaggtgt aacttgtgaa aaatgcggcg tggaagtaac cctgtccaaa
     8221 gtgcgccgcg aacgcatggg ccatatcgaa ttggctgcgc ccgtcgcaca tatttggttc
     8281 ttaaaatccc tgccttcccg cttgggtatg gtgttagaca tgactttgcg cgatatcgaa
     8341 cgcgtattgt actttgaagc atttgtggta accgatcccg gtatgactcc gctgcaacgc
     8401 cgccaattgc tgactgaaga cgattactac aacaagctgg acgaatacgg cgacgacttc
     8461 gatgccaaaa tgggtgcaga aggtatccgc gaattgctgc gtaccttgaa tgtagcaggc
     8521 gaaatcgaaa tcctgcgcca agagttggaa tcgaccggtt ccgacaccaa aatcaaaaaa
     8581 atcgccaaac gcttgaaagt attggaagct ttccatcgtt ccggtatgaa gctggaatgg
     8641 atgattatgg atgtgctgcc ggtattgccg cctgatttgc gtccgttggt tccattggat
     8701 ggcggtcgtt ttgccacttc cgatttgaac gatttgtacc gtcgcgtcat caaccgtaac
     8761 aaccgtctga aacgtctgtt ggaactgcat gcgcccgaca ttatcgtccg caacgaaaaa
     8821 cgtatgttgc aagaagcagt tgactcgctg ttggataacg gccgtcgcgg taaagccatg
     8881 accggcgcca acaaacgccc gctgaaatca ttggcagaca tgattaaagg taaaggcggt
     8941 cgcttccgtc aaaacctgtt gggcaaacgt gtggactact ctggccgttc cgtgattacc
     9001 gtaggcccat acctgcgtct gcaccaatgc ggtctgccga aaaaaatggc tttggaattg
     9061 ttcaaaccgt tcattttcca caaattggaa aaacaaggtt tggcttctac tgttaaagct
     9121 gcgaaaaaat tggtagaaca agaagtacct gaagtatggg atatcttgga agaagtcatc
     9181 cgcgaacatc cgattatgtt gaaccgtgcg ccaaccctgc accgtttggg tattcaagcg
     9241 ttcgaaccca tcctgattga aggtaaagcc attcagttgc atccgttggt atgtgccgca
     9301 tttaacgccg actttgacgg cgaccaaatg gcggtacacg ttccattgag cttggaagca
     9361 caaatggaag cacgcacgct gatgctggct tcaaacaacg tattgtctcc tgccaacggc
     9421 gaaccgatta tcgtaccttc ccaagacatc gtattgggtc tgtactacat gacccgcgac
     9481 cgtatcaatg ccaaaggcga gggcagcctg tttgccgatg tgaaagaagt gcatcgcgca
     9541 taccatacca aacaggtcga gctgggtacg aaaattaccg tacgtctgcg cgaatgggtg
     9601 aaaaacgaag caggtgagtt tgagcctgtc gttaaccgtt acgaaacaac cgtcggccgt
     9661 gcattgttga gcgaaatcct gccgaaaggc ctgccgtttg aatatgtcaa caaagcgttg
     9721 aagaaaaaag aaatttctaa actgattaac gcatcgttcc gcctgtgcgg cttgcgcgat
     9781 acggttatct ttgctgacca cctgatgtac accggtttcg gatttgcggc aaaaggcggt
     9841 atttccattg ccgttgacga tatggaaatt ccaaaagaaa aagcggcctt gctggctgaa
     9901 gccaatgccg aggttaaaga aatcgaagac caataccgtc aaggtttggt taccaacggc
     9961 gaacgctaca acaaggtggt cgatatttgg ggtcgtgccg gcgataaaat cgctaaagcg
    10021 atgatggaca acctgtccaa acagaaagtt atcgaccgtg acggcaacga agtcaatcaa
    10081 gagtcattca actccatcta tatgatggcg gactccggtg cccgtggttc tgcggctcag
    10141 attaaacagt tgtccggtat gcgcgggttg atggctaaac ctgacggctc gattattgaa
    10201 acgccgatta cctcaaactt ccgcgaaggt ttgaccgtat tgcaatactt tattgcgact
    10261 cacggtgcgc gtaaaggttt ggcggatacc gcgttaaaaa ccgcgaactc cggttacctg
    10321 acccgtcgtc tggtagacgt aactcaagat ttggtcgttg ttgaagacga ttgcggtact
    10381 tcagacggct ttgtcatgaa ggcagtggta caaggcggtg atgtgattga agcattgcgc
    10441 gatcgtattt tgggccgtgt tactgcgtct gacgttgtcg atccgtcaag cggcgagacc
    10501 ttggttgaag ccggtacgtt gttgactgaa aaactggtgg atatgattga ccaatccggt
    10561 gtcgatgaag tcaaagtccg tactccgatt acttgtaaaa cccgccacgg tttgtgcgcg
    10621 cactgttatg gtcgcgactt ggcgcgcggt aagctggtta acgccggtga agcagttggt
    10681 gtgattgctg cacaatccat tggtgaaccg ggtacccagt tgaccatgcg tacgttccac
    10741 attggtggtg cggcatcccg tgcggcagca gccagccaag tggaagccaa atccaacggt
    10801 acggcacgtt tcagcagcca gatgcgttac gttgccaaca acaaaggtga gttggttgtc
    10861 atcggtcgct cttgcgaagt cgtgattcat gatgacatcg gccgtgaacg cgaacgccac
    10921 aaagtacctt acggtgccat cctgctggta caagacggag cggccattaa agccggtcaa
    10981 accttggcaa cctgggatcc gcatacccgt ccgatgatca ccgaacatgc aggtatggtg
    11041 aaatttgaga acgtggaaga gggtgttacc gttgcgaaac aaactgacga tgtaaccggt
    11101 ttgtccactc tggtagtgat tgacggtaaa cgtcgttcct ctagtgcttc taaactgctg
    11161 cgtccgactg taaaattgtt ggatgaaaac ggcgtggaaa tctgtattcc cggtacgtcc
    11221 actccggttt cgatggcatt ccccgttggt gcggtgatta ccgtacgcga aggtcaggaa
    11281 atcggtaaag gcgacgtatt ggcgcgtatc ccgcaagcct cttccaaaac ccgcgacatt
    11341 accggtggtc tgccgcgcgt tgccgaattg tttgaagcac gcgtgccgaa agatgccggt
    11401 atgttggcgg aaattaccgg taccgtttcc ttcggcaaag agaccaaagg caagcaacgt
    11461 ctgattatta ccgacgtgga cggcgtagca tacgagactt tgatttccaa agagaaacag
    11521 attttggtac acgacggtca agtggtaaac cgcggcgaaa ccattgtgga cggcgcggtc
    11581 gatccgcacg atattctgcg tctgcaaggt atcgaagcac tggcacgcta cattgtccaa
    11641 gaggtgcaag aggtttaccg tctgcaaggt gtgaagattt ctgataaaca catcgaagtc
    11701 atcatccgtc aaatgttgcg ccgtgtgaac attgcggatg ccggcgaaac cggcttcatt
    11761 accggagagc aggtcgaacg cggcgatgtg atggcggcca atgaaaaagc tttggaagaa
    11821 ggcaaagaac cggcgcgtta cgaaaacgta ttgctgggta ttaccaaagc ttccctgtct
    11881 accgacagct tcatttctgc tgcatcgttc caagaaacga cccgtgttct gaccgaagcc
    11941 gcgattatgg gcaaacaaga cgaactgcgc ggtttaaaag agaacgtcat cgtcggccgc
    12001 ctgattcctg ccggtaccgg tttgacttat caccgcagcc gtcatcagca atggcaaggg
    12061 gtagagcagg agactgctga aacccaagta acggatgaat aatcttcggc gcgtttattc
    12121 aatacaaaac cgcaagcttt aagcttgcgg tttttctttg tccgattaag gcaaaaacaa
    12181 gcgttttcgt cattttgagg cgtgtggatt attccttagg tattttcggg ccggagacca
    12241 acgaggtggc gggtgtcgtc ggtacgtccg gagaccaaaa taactttgcc agggatgttg
    12301 gtttcggcgg tcaaaaaaag tagcgtctta atgttttcca tttaaacaaa tgtcgtctga
    12361 aacttcagac ggcatttcct ttaaaaaata aatatgaaac ccagaaatct cttttttgca
    12421 ggctgcctgc tgacttcggc gacgtttgcc gaggatatcg gcgtacctgt cgaactgatt
    12481 aacgtcggta atcggattgc gatgccgtct gaaggggaaa gcctcgccct cctgccgttt
    12541 gccgaggatg taccgccggt tcgcgatgca atgccgtctg aagttcctaa aagcgcggca
    12601 ggcggcgatg ttcggggtaa ccggatggga atgccgatta acatcggatg agcgcggctt
    12661 tatggcataa aaaactgtcg tggaaaggat ttacacccca aataaatttc cgttacaaca
    12721 agatcaacag caatatgccc gccttttatt cgcgcagcgg caaggaacgg tttgtcagta
    12781 tagaaaaaac gtattgacag tattttcttc agtcgtccga ctgattgtga gggatgttgg
    12841 taaatattta tcggcaaaca agaaaatcat ctttcttctt gtcgttatgc ttgactgtat
    12901 acttgcaata aaaatataat tccactcttg ccgacatggt gtcggcaagt atttaactca
    12961 acaggacgag aaaatatgcc aactatcaac caattggtac gcaaaggccg tcaaaagccc
    13021 gtgtacgtaa acaaagtgcc cgcactggaa gcctgcccgc aaaaacgcgg cgtgtgcacc
    13081 cgtgtataca cgactacccc taaaaaacct aactctgcat tgcgtaaagt atgtaaagtc
    13141 cgcctgacca acggttttga agtcatttca tacatcggcg gcgaaggcca caacctgcaa
    13201 gagcacagtg tcgtattgat tcgcggcggt cgtgtaaaag acttgccagg tgtgcgttac
    13261 cacactgtac gcggttcttt ggatactgca ggtgttaaag accgtaaaca agcccgttcc
    13321 aaatatggtg ctaagcgtcc taaataatta ctgggactta aataggcacg tcggccgcct
    13381 aagcagaaca acggccgagt aagtgaatac tcaattgggt attcatggga atagacccga
    13441 ctgaatagat taaaggaaat taaaatgcca agacgtagag aagtccccaa gcgcgacgta
    13501 ctgccagatc ctaaattcgg cagcgtcgag ttgaccaaat tcatgaacgt attgatgatt
    13561 gacggtaaaa aatccgttgc cgagcgtatc gtttacggtg cgttggaaca gattgagaaa
    13621 aaaaccggca aagtagcaat cgaagtattt aacgaagcca ttgcaaacgc caaacctatc
    13681 gtggaagtga aaagccgccg tgtaggtggt gcaaactacc aagttcctgt tgaagttcgt
    13741 ccttcacgcc gtttggcttt ggcaatgcgc tgggttcgcg atgcggcccg caaacgtggt
    13801 gagaaatcca tggacctgcg tttggcaggc gaattgattg atgcgtccga aggccgtggc
    13861 ggtgcgttga aaaaacgtga agaagtacac cgtatggctg aagccaacaa agcattctct
    13921 cacttccgtt tctaattttg aaaggctaat aaaatggctc gtaagacccc gatcagcctg
    13981 taccgtaaca tcggtatttc cgcccatatc gatgcgggta aaaccacgac gacagaacgt
    14041 attttgttct ataccggttt gacccacaag ctgggcgaag tgcatgacgg tgcggctact
    14101 accgactaca tggaacaaga gcaagagcgc ggtattacca ttacctccgc tgccgttact
    14161 tcctactggt ccggtatggc gaaacaattc cccgagcacc gcttcaacat catcgacacc
    14221 ccgggacacg ttgactttac cgtagaggta gagcgttcta tgcgtgtatt ggacggcgcg
    14281 gtaatggttt actgcgcggt gggcggtgtt caaccccaat ctgaaaccgt atggcggcaa
    14341 gccaacaaat accaagtgcc gcgcttggcg tttgtcaata aaatggaccg tcagggtgcc
    14401 aacttcttcc gcgttgtcga gcaaatgaaa acccgtttgc gcgcaaaccc tgtacctatc
    14461 gtcattccgg ttggtgcgga agacaacttc agcggtgtgg ttgatttgtt gaaaatgaaa
    14521 tccatcattt ggaatgaagc cgataaaggt acaaccttta cctatggcga tattcctgcc
    14581 gaattggtcg aaactgccga agaatggcgt caaaatatga ttgaagccgc agccgaagcc
    14641 agcgaagaac tgatggacaa atacttaggc ggcgacgagc tgaccgaaga agaaatcgta
    14701 ggcgcgttgc gtcaacgtac tttggcaggc gaaattcagc ctatgctgtg tggttctgca
    14761 tttaaaaaca aaggtgttca acgtatgttg gacgcagttg tagaattgct gccagctcct
    14821 accgatattc ctccggttca aggtgtcaac ccgaataccg aggaagccga cagccgtcaa
    14881 gccagcgatg aagagaaatt ctctgcattg gcgttcaaaa tgttgaacga caaatacgtc
    14941 ggtcagctga cctttatccg cgtttactca ggcgtagtaa aatccggcga taccgtattg
    15001 aactccgtaa aaggcactcg cgaacgtatc ggtcgtttgg tacaaatgac tgccgcagac
    15061 cgtactgaaa tcgaagaagt acgcgccggc gacatcgcag ccgctattgg tctgaaagac
    15121 gttactaccg gtgaaacctt gtgtgcggaa agcgcgccga ttatcttgga acgtatggaa
    15181 ttccccgagc cggtaatcca tattgccgtt gagccgaaaa ccaaagccga ccaagagaaa
    15241 atgggtatcg ccctgaaccg cttggctaaa gaagaccctt ctttccgtgt ccgtacagac
    15301 gaagaatccg gtcaaaccat tatttccggt atgggtgagc tgcacttgga aattattgtt
    15361 gaccgtatga aacgcgaatt cggtgtggaa gcaaatatcg gtgcgcctca agtggcttac
    15421 cgtgaaacta tccgcaaagc cgttaaagcc gaatacaaac atgcaaaaca atccggtggt
    15481 aaaggtcaat acggtcacgt tgtgattgaa atggaaccta tggaaccggg tggtgaaggt
    15541 tacgagttta tcgatgaaat taaaggtggt gtgattcctc gcgaatttat tccgtctgtc
    15601 gataaaggta tccgcgatac gttgcctaac ggtatcgttg ccggctatcc tgtagttgac
    15661 gtacgtatcc gtctggtatt cggttcttac catgatgtcg actcttccca attggcattt
    15721 gaattggctg cttctcaagc gtttaaagaa ggtatgcgtc aagcatctcc cgccctgctt
    15781 gagccgatta tggcagttga agtggaaacc ccggaagaat acatgggcga cgtaatgggc
    15841 gacttgaacc gccgtcgcgg tgttgtattg ggtatggatg atgacggtat cggcggtaaa
    15901 aaagtccgtg ccgaagtacc tctggcagaa atgttcggtt actcgaccga cctgcgttct
    15961 gcaacccaag gccgcgctac ttactctatg gagttcaaga aatattctga agctcctgct
    16021 cacatagctg ctgctgtaac tgaagcccgt aaaggctaat caggcaaata ggccgtccga
    16081 aaggctgaaa tgatttttca gacggcattg ttctttaatc gatctttaat gtaaaggaat
    16141 tagc
//
LOCUS       DAXIHS010000044        15955 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXIHS010000044 DAXIHS010000000
VERSION     DAXIHS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.044"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..216
                     /locus_tag="ACFOF2_001223"
     CDS             <1..216
                     /locus_tag="ACFOF2_001223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107543.1"
                     /translation="HEPPSRHYHESGNLGRRVKKTYIPSFPHKRESKNKKSQKFNGRT
                     EPRKSASRLRLNNRTKEAEIKTSGFFF"
     gene            325..4977
                     /gene="iga"
                     /locus_tag="ACFOF2_001224"
     CDS             325..4977
                     /gene="iga"
                     /locus_tag="ACFOF2_001224"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC7107544.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QHISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     EKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAAEIAE
                     QKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREAAELA
                     KQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEIPLDA
                     LEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAV
                     STNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQ
                     YRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYL
                     NDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYS
                     RLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGV
                     NVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5156..6358)
                     /gene="trpB"
                     /locus_tag="ACFOF2_001225"
     CDS             complement(5156..6358)
                     /gene="trpB"
                     /locus_tag="ACFOF2_001225"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC7107545.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(6441..7130)
                     /locus_tag="ACFOF2_001226"
     CDS             complement(6441..7130)
                     /locus_tag="ACFOF2_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC7107546.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            7327..8106
                     /gene="rsmA"
                     /locus_tag="ACFOF2_001227"
     CDS             7327..8106
                     /gene="rsmA"
                     /locus_tag="ACFOF2_001227"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC7107547.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8279..9007
                     /locus_tag="ACFOF2_001228"
     CDS             8279..9007
                     /locus_tag="ACFOF2_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC7107548.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9080..9439
                     /locus_tag="ACFOF2_001229"
     CDS             9080..9439
                     /locus_tag="ACFOF2_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107549.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            9444..9890
                     /locus_tag="ACFOF2_001230"
     CDS             9444..9890
                     /locus_tag="ACFOF2_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC7107550.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLKITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAATLAGSSMVTPYALF
                     LQWDTGGKTVRHCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            9921..11195
                     /gene="folC"
                     /locus_tag="ACFOF2_001231"
     CDS             9921..11195
                     /gene="folC"
                     /locus_tag="ACFOF2_001231"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC7107551.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            11208..12206
                     /gene="ftsN"
                     /locus_tag="ACFOF2_001232"
     CDS             11208..12206
                     /gene="ftsN"
                     /locus_tag="ACFOF2_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC7107552.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESRKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            12199..12696
                     /locus_tag="ACFOF2_001233"
     CDS             12199..12696
                     /locus_tag="ACFOF2_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC7107553.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            12904..14448
                     /gene="purF"
                     /locus_tag="ACFOF2_001234"
     CDS             12904..14448
                     /gene="purF"
                     /locus_tag="ACFOF2_001234"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC7107554.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            14548..15039
                     /gene="greB"
                     /locus_tag="ACFOF2_001235"
     CDS             14548..15039
                     /gene="greB"
                     /locus_tag="ACFOF2_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC7107555.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            15098..15724
                     /locus_tag="ACFOF2_001236"
     CDS             15098..15724
                     /locus_tag="ACFOF2_001236"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC7107556.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4435 a         4322 c         3896 g         3302 t
ORIGIN      
        1 cacgaacccc catctcgtca ttaccacgaa agcgggaatc taggacgcag ggttaagaaa
       61 acctacatcc cgtcattccc gcacaagcgg gaatccaaaa acaaaaagtc gcagaaattt
      121 aacggaagaa ccgaaccccg aaaatcggct tcccgccttc gcttgaataa cagaacaaag
      181 gaagcggaaa taaaaacatc gggattcttt ttttaaattg acaataaaac accaaaatta
      241 atgatttaca caacaaaaaa tagtcaacac cgccaaaccg gcgtaaaata caagacatta
      301 tcacccttaa aacggtaaaa ccttatgaaa accaaacgtt ttaaaattaa cgccatatcc
      361 ttatccatct ttcttgccta tgcccttacg ccatactcag aagcggcatt ggtcagagac
      421 gatgtcgatt atcaaatatt ccgtgacttt gcagaaaaca aaggcaaatt ttttgtcggc
      481 gcaaccgatt tatcagtgaa aaacaaacaa ggtcaaaaca tcggcaacgc attatccaac
      541 gtaccgatga ttgattttag cgttgcagat gtcaacagac gtacattaac cgtcatcgat
      601 ccccagtatg ccgtcagcgt caaacatgta aaaggagacg aaatctctta ttacgggcat
      661 cacaatgggc acttagatgt tagcaatgat gaaaacgaat atcgttcagt tgcacaaaat
      721 gactacgaac caaataaaaa ttggcatcac ggtaatcaag gtcgtcttga agactacaac
      781 atggcacgcc ttaataaatt cgtaacagaa gtcgcaccta ttgcaccaac cagtgcaggc
      841 ggcggcgttg aaacctataa agataaaaac cgtttttctg agtttgtgcg agttggagca
      901 ggtacgcaat ttgaatataa cagccgctac aatatgacag agctttcacg agcttatcgt
      961 tatgccattg caggtacgcc ttatcaagac gtgaatgtca catcgaatct aaatcaagaa
     1021 ggcttgattg gcttcggtga taattcaaaa catcattccc cagaaaaact caaagaagtt
     1081 ttatctcaaa acgcattgac taactatgct gtgttaggcg atagcggttc accattattt
     1141 gcttatgata aacaagaaaa acgctgggtc tttttaggtg cttacgatta ttgggcaggc
     1201 tatcaaaaaa actcttggca agaatggaat atctataaaa aagaatttgc agatgaaatc
     1261 aaacaacgcg ataacgccgg caccatcaaa ggttatggcg aacatcattg gaaaaccacg
     1321 ggcacaaaca gccatatcgg ttcgacagcg gtaaggcttg ccggcaatga aaggggtgca
     1381 aacaacggac agaatgttac ctttgaaaac aacggcactt tggtattgga tcaaaacatc
     1441 aaccaaggcg cgggcggtct gtttttcaaa ggcgattaca cagtcaaagg tataaataat
     1501 gacatcactt ggctaggtgc ggggattgat gttgccgacg gcaaaaaagt cgtttggcaa
     1561 gtcaaaaatc cgaatggcga cagattggcg aaaatcggca aaggcacatt agaaataaac
     1621 ggcacaggcg ttaaccaagg gcaattaaaa gtcggcgacg gtacggttat tctgaatcaa
     1681 aaagccgatt caaaccaaaa agtccaggct ttctcccaag tcggcattgt cagcggacgc
     1741 ggtacattgg tattaaatag ttcaaatcag attaatcccg ataacctata tttcggtttc
     1801 cgtggcggtc gtttggatgc caatggcaat gacttgactt ttgaacacat ccgcaacgtg
     1861 gatgaaggcg cgcgcattgt caaccacaac acaggccaca cctccacaat cacgctaacg
     1921 ggtaaatctt taattaccaa tccgaacagc ctttcggtac atagtataca aaatgactat
     1981 gatgaagatg attatagtta ttattaccga ccaaggcgac ccatcccaca aggcaaagat
     2041 ctttattaca aaaactatcg ttattacgcc ctaaaatccg gcggcagcgt gaacgcaccg
     2101 atgcccgaaa acggacaaac ggaaaataac gactggattt taatgggcag cacacaagaa
     2161 gaagccaaga aaaacgcgat gaaccacaaa aacaatcagc atattagcgg ttttagcggt
     2221 ttctttggcg aagaaaacgg aaaaggacat aacggtgcat taaaccttaa tttcaacggc
     2281 aaaagcgcgc aaaaccgttt cttgttaaca ggtggcacta atttaaacgg aaaaataagc
     2341 gtaactcaag gcaatgtctt gttatcaggt cgtccaacac cacacgcaag agattttgtg
     2401 aacaaatctt cagcccgaaa agacgcacat ttctccaaaa acaatgaagt cgtatttgaa
     2461 gacgactgga taaaccgcac attcaaagcc acagaaattg cggttaatca atccgcatca
     2521 ttctcttccg gcagaaatgt atccgacatc accgccaaca tcaccgcaac agacaacgcc
     2581 aaagtaaatt tgggttacaa aaacggcgat gaggtttgcg tgcgctcgga ctataccggt
     2641 tatgttacct gcaacacagg caacttatcc gataaggctt taaacagctt tggagcgaca
     2701 cagattaacg gcaatgtgaa tctgaaccag aatgcggcat tggtcttggg caaggctgca
     2761 ttatgggggc aaattcaagg gcaaggaaac agccgtgtca gcctaaacca acatagcaaa
     2821 tggcatttga ctggcgacag ccaagtacac aatctgtcat tggcggatag ccatattcat
     2881 ttgaacaatg cctccgatgc gcaaagtgca aatcaatacc acacgctcaa aatcaatcat
     2941 ttatccggta acgggcattt tcattatctg acgcacctgg cgaaaaatct tggggataaa
     3001 gtgcttgtga aggaatccgc atccggtcat tatcagctcc atgttcaaga taaaacaggc
     3061 gaacccaatc aggaagggct gaatctcttt gatgcatcat ccgtacaaga ccgctcccgc
     3121 ctttctgttt ccttggcgaa taaccatgta gatttagggg cattgcgtta tacaatcaaa
     3181 acagaaaacg gtattacccg attgtacaat ccttatgccg agaaccgccg ccgagtcaaa
     3241 ccggccccgt ctcctgccac aaacacggct tctcaagcac aaacggacag tgcacaaatt
     3301 gccaagcctc aaaatatcgt cgtcgcaccg cctagcccgc aggcaaatca agccgaagaa
     3361 gcaaaacgcc aacaagcaaa agcggagcaa gtgaagcgtc agcaagcaga ggcagaaaaa
     3421 gtcgcacatc aaaaggctga agaagcgaaa cgtcagcaag atgcccttgc ccgccaacaa
     3481 gcagagcaag aacgccaacg attggaagcc gaacgccaag ctgccgaaat tgccaaacaa
     3541 aaagcagaag ccgaagaagc gaaacgccga gccgctgaaa ttgccgagca aaaagcagct
     3601 gccgaagaag caaaacgcca agctgccgaa cttgcacgcc aacaagaaga agcacgaaaa
     3661 gctgcagagt tggctgctaa gcaaaaagcg gaaactgaac gcaaagcagc agaaattgcc
     3721 gagcaaaaag cggaagccga acgtgaagcc gccgaacttg ccaagcaaaa ggcggaagaa
     3781 gaaggtcgcc aagcagcaca atctcaaccc aaacgtcgta accgcagggc tataccaccg
     3841 gaactgtcat ctgatgcaac aacccgagct ttgcctagga tagcgagaaa ttcaaatcct
     3901 gatgccagcg attatgaaga aatccccttg gatgcgttgg aagatgaaga tgtatccgaa
     3961 tcggttgaca catcagacaa acagcctcaa gacaatacgg aacttcatga aaaagttgag
     4021 acagtgagtt tgcaaccaag agccgctcag ccgcgagccc aagccgccgc gcagccgcaa
     4081 gcccaagccg tcgcgcaagc cgatgcagtc agcaccaata ccaactcggc tttatctgac
     4141 gcaatggcaa gcacgcaatc tatcttgttg gatacaggtg cttcattaac acggcacatt
     4201 gcacaaaaat cacgcgctga tgccgaaaaa aacagcgttt ggatgtcgaa catcggttat
     4261 ggccgtgatt atgcttccgc acaatatcgc cggtttagtt cgaaacgcac gcaaacacaa
     4321 atcggcattg accgcagctt gtccgaaaat atgcagatag gcggcgtatt gacttactct
     4381 gacagtcagc atacttttga tcaggcgagc ggcaaaaata cttttgtgca agccaacctt
     4441 tatggtaagt attatttaaa tgatgcttgg tatgtggccg gcgatattgg tgcaggcagc
     4501 ttgagaagcc ggttacaaac acagcaaaaa gcaaacttta accgaacaag catccaaacc
     4561 ggccttactt tgggcaatac gctgaaaatc aatcaattcg agattgtccc cagtgcggga
     4621 atccgttaca gccgcctgtc atctgcagat tacaagttgg gtaacgacag tgttaaagta
     4681 agttctatgt cagtgaaaac actgacggcc ggactggatt ttgcttatcg gtttaaagtc
     4741 ggcaacctta ccgtaaaacc cttgttatct gcagcttact ttgccaatta tggcaaaggc
     4801 ggcgtgaatg tgggcggtaa ttccttcgcc tataaagcag ataatcaaca gcaatattca
     4861 gcaggtgccg cgttactgta ccgtaatgtt acattaaacg taaatggcag tattacaaaa
     4921 ggaaaacaat tggaaaaaca aaaatccgga caaattaaaa tacagattcg tttctaaaat
     4981 actaaattca tagcaaaata aaatgccgtc tgaactcagg cttcagacgg catttttata
     5041 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggca
     5101 aggcgaggca acgccgtact ggtttttgtt aatccactat atcagacgga cgaggttaca
     5161 gtttaatccc tttgagcttc gcgacggtat tgatgtcttt gtcgccacga ccggataggt
     5221 tgaccagaat cacttggtct ttgcccattt tcggcgcgtt tttcaccgcc caagcaacgg
     5281 cgtggctgga ttcgagcgcg gggatgatgc cctcgaagcg gcagagcaaa tcaaaggctt
     5341 cgagtgcttc gtcgtcttta gcgacggtgt attcgacgcg cttgatgtcg tgcagatggc
     5401 tgtgttccgg gccgatgccg gggtaatcca agcctgcgga aacagagtgt gtacccaaga
     5461 cttgaccgtt ttcgtcctgc atcagatagc tgcggaaacc gtgcaatacg ccaatcggtg
     5521 agcccgaagt aatcggcgcg gcgtgatcgg gggtgttcac gcccaaaccg ccagcctcca
     5581 cgccgacgag gcgcacgttt tcttcgccga tatacgggtg gaacaaacca atggcgttcg
     5641 atccgccgcc cacgcaggca acggcaacgt cgggctgtct gccgatggct tccaacattt
     5701 gttctttggc ttcattgccg atcacgcatt gaaagtcgcg caccatttcg ggatacggcg
     5761 cgggaccggc ggcggtaccg atgatgtaga atgtgtcatc cacgcgggcg acccattcgc
     5821 gcatggcttc gttcatcgcg tctttcaaag tacggctgcc gctgtcaacg ccgacgacat
     5881 tcgcgcccaa taatttcata cggaacacgt tgggcatttg gcgttggatg tcgtccgcac
     5941 ccatgtacac gtcgcaagtc atgccgaagc gggcggcaac agtggcactc gccacgccgt
     6001 gctgacccgc gccggtttcg gcgatgacgc gttttttacc catacgtttt gccaacagtg
     6061 cctgaccgat ggtgttgttg actttgtgcg cgccggtgtg gttcaagtct tcgcgcttca
     6121 accagatttg cgcaccgccc aaatgctcgg acaaccgcgc ggcgtggtaa acggggctgg
     6181 gcctgccgac ataatgtttc aaatcgcggt ggaattcttc ccaaaacgac gggtcgtttt
     6241 tcgctgcctt ataggcatcc gccagctctt gcaaagcggg aatcagggtc tcggacacga
     6301 acagcccgcc gtgttcgccg aaaaagccct tctcgtcggg cgcgtggtag ttcttcatcg
     6361 gattcttcct tgtgcttcag gttttcaaac ggcatttccg ttttttcaaa catgccgtcc
     6421 gaacgggaaa ttgtcggcaa ttataactgc ttttcacaca agcccgccat ttcgaaaaac
     6481 gcccgataca ccgccgcctt tttcgccaaa ctcaaaggat aggcgcgcga agtcgaaggc
     6541 aggcgcgtca gcgtcaaatc ccgtccggca aacggaaacg gcacggtttc gcccgttttc
     6601 ggcattttga tgccgccacc ctgaatatcg agcaggattt ccgtcgcctt gccgcctgtc
     6661 gtacaaatat ggcggcactc gggtattttt gccaacaccg ccgccaaatc gacggtttca
     6721 actaccttta aaaacttgtc ggacgcattg ccgtgttccc ttgccgcctt caacacggtc
     6781 ggacaggacg caatcccccg ttcgtgcaaa aacgccttga ttttctcggc atcaaacgct
     6841 ttttcagaca acctttggaa atgcgccgca tcattaaaaa acaccagccc gtaaacgcgc
     6901 cacatatcgt tttggaaatt cggataatga aactgcatcg cgcgtttgtc ttccttgggc
     6961 ggaaacgtcc ccatcatcat gaccgcagcc ttcggcggca acacggaatc gaagggatgg
     7021 gtttcgattt caagcagatt ttcagacacg ttcaatcctc caacaggcaa aacggcagac
     7081 agccggtttt tgcaccaaac cgccaaacac ggcaagcctt caaacagcat tatcacgccc
     7141 tgccgttttc ggttacaatg ccgacatcaa acggatactg taaacacatt tatgttccaa
     7201 cacacaggac gacacttaaa gcaccgccct atgtgccatc ctgatttgga aggagttacg
     7261 cccctcccaa atagagtctg attctaccgc cctaaagggc ggggtttcaa ccgaaaagga
     7321 aacacgatga aagaacacaa agcccgcaag cgtttcgggc agaatttttt gcaggacacg
     7381 cggatcatca gcgatattgt caacgccgtg cgcccgcagg cggatgatgt cgtgattgaa
     7441 atcggcccgg gtttggcggc gattaccgaa cctttggcga aaaagctgaa ccgcctgcac
     7501 gttgtcgaaa tcgaccgcga catcgtatgc cgtctgaaaa cgctgccgtt tgcggataaa
     7561 ctggtgattc acgaaggcga tgtattgcag tttgatttca acggcatcgc aggcaaaaag
     7621 aaaatcgtcg gcaacctgcc ctacaacatt tccacgccgc ttttgttcaa gctggcggag
     7681 gtggcggacg atgtcgtcga tatgcacttt atgctgcaaa aagaagtggt cgatcgtatg
     7741 gttgccgcgc cgaaaagcaa cgactacggc cgcttgggcg tgatgctgca atatttcttc
     7801 gatatggaaa tgctgattga cgtgccgccc gaatcgttcg accccgcgcc gaaagtggat
     7861 tcggccgtcg tccgcatgat tccggtgaaa caccgtatcg gcaaggcgga cgatttcgag
     7921 catttcgcca aactcgtgaa actcgccttc caccaacgcc gcaaaaccat acgcaacaat
     7981 ctgaaagagc ttgcaggcga cgatgatttg caggcagtcg gcatcaatcc gcaagaccgc
     8041 gccgaacata tcgcgccgga gaaatatgtg gcgttgagca attatctggc gggcaaggcc
     8101 gtctgaaaag ggagtaagcc gatgacgaaa tacctgcttt tacccctgat actgccgctt
     8161 gcagcctgcc tgaccaatcc gaacgaccgt ttttttgacg gcagacgcta cacgcttcat
     8221 catatggagc tcccgtaacg acaacctgca acggcaaatg ccgtctgaaa gaaaagacat
     8281 gatcaaattc aaaaacgtac acaaacattt caaagacctg cacgtcatca acggcgtgaa
     8341 cttggaaatc aaaaaaggcg aagtggtcgt cgtctgcgga ccttcgggca gcggcaaatc
     8401 cacactcatc cgcaccgtca accagcttga aagcatcgaa agcggcgaga tttgggtgga
     8461 cggtgtcaac gttgccgacc caaaaaccga tttgaacaaa atccgcgaag aagtcggctt
     8521 tgtgtttcaa agtttcaacc tctaccccca cctgaccgta ttggaaaaca tcaccctcgc
     8581 accgatgaag gttaaaggtc aaaatgcgga acaggcggag aaaaaagcga tggaactttt
     8641 ggaacgcgtc ggactggcgc acaaaaaaga cgcttttccg tcccaacttt ccggcggcca
     8701 gcagcagcgc gtggcgattg cacgcggttt ggcgatggaa ccgcgcgtaa tgttgtttga
     8761 cgaaccgacc tccgcactcg accccgaaat ggtcggcgaa gtgttgaaag tgatgaaaga
     8821 cttggcagaa agcggcatga ccatgatgtg cgtaacccac gaaatggggt ttgcccgcga
     8881 agttgccgac cgcgtgattt tcgtcgataa agggcaaatc ctcgaagacg aaacgcccga
     8941 agcgtttttc accaacccga aacacgaacg cgccaagcag ttcctgcaac aggtcatgac
     9001 ccactgatgc tataccgtct gaaaaccgaa cgttcagacg gcatagcccg acaccgcaag
     9061 atgattgaga aagaaaacca tgtattttgt tgaccgcacc gccgtcgtcc tcaagccgac
     9121 cgtccgcttt ttggaatggc tcaaaagcac cgacgaaaat atgcccgacc tgaccataga
     9181 gcagcttcgt gccaactgct ccgtcttcct cgttccgcag ttcgacgaac cggaagccgt
     9241 cgtttcctat ttcgacgaac gctaccgcca gatttttgaa gccgaactcg ccggctggga
     9301 catcgacaag gataaatggc cgcaagatat ggggctgaag gcgttttggg aatttttcga
     9361 catcgaaatc cacgatatgg tgttggatat ggaagaagcg gatttgaaca tcacgcccgt
     9421 gttcgacaat atgatgtaac gccgtgcgcg cctttcagac agcattgagg ccgtcccgca
     9481 tcctgaacat cctgaccgta tcgctccact cggcttccct tgccgtctgc ctgacctggt
     9541 tttacggccg gatgatgtgg ttcggtttgg cggctttggc ggcaagttac gcatactcgc
     9601 tcaagataac gaatctgaaa caccgccatg ccataaccgc cattaccata gaccgcgacg
     9661 gacaggcgga aatcgtatcc ggcaaagaaa aaacagcaaa ggcggcaaca cttgcgggca
     9721 gcagtatggt tacgccttat gccttattct tacaatggga caccggcggc aaaaccgtcc
     9781 gccactgcat catgcccgac atgacggaca aagaatccta ccgcaagctg aaagtctggg
     9841 tactttggcg gcagccgaaa aaaaccgccg aaaccgatac atcagactga tttcagacgg
     9901 catttgaaaa cgaaacactc atgaaaacac tacaagactg gctctcgcat ttggaaaccg
     9961 cccacagcgg cggtttgatc gacatgggtt tggagcgcac gagcgaagtg aaaaaacgca
    10021 tgaagctcga gccgcaatgc cccgtcgtcg ttgtcgcggg aaccaacggc aaaggttcgg
    10081 tctgtgccta cctgacacag atttacaaac aggcgggcta caaaaccggt acgctgacca
    10141 gcccgcattt attgcgttac aacgaacgca tcgccatcaa cgccgaaccc gtttcggacg
    10201 atacgattat tgcctctttc gagcgcatcg aagcggcgcg cagcgaaata tcgctgactt
    10261 attttgaatt caatacgttg gcggccgtcg acatcttcat gcgcgaacag gttgacgtga
    10321 tgatattgga agtcggcttg ggcggacgct tggacgcggt caacgcattt gacggcgatt
    10381 gcgcggtggt tactagtgta gatttggacc atcaggcgtt tttgggcgat acggtcgagc
    10441 aggtcggctt tgaaaaagca ggcgtgttcc gcagcggcaa acccgcaatc tgcggtcaaa
    10501 accccgcgcc cgaatcgttg ctttcacacg ccgaagccat aggcgcgaaa ctgctgatgg
    10561 tgcagcgcga tttcgagttt cacgcgatgg aaaacatcca atggaactac cgcttccggc
    10621 cgcaacattc agacgacccc gcgcgcaacc gcaatgccct gcccttcccc gcattgcgcg
    10681 gcacatacca actttccaac gccgcctgca cgttgaccgt attggaatgc ttgaacgcca
    10741 aattgccggt ggacatcggc gccatcaaac gcggattgct gctggttgaa aatcccggac
    10801 gcttccaagt cctgcccggc cgtccgctga ccgttttgga tgtcggacac aatccccacg
    10861 ctgcccgcgc cctgcgccgc agcctgatta atttggcgtt tgcgcaaaaa cgcaccgccg
    10921 tgttcagcat gttgtccgac aaagacatag acggcgtgtt ggaaaccgtt aaagaccagt
    10981 ttgacgagtg gtatatcgca ccgttggatg taccgcgcgg catgacgctc gagagcctgc
    11041 aaaacaaact gcacgaacac agcatcgaaa acatacaaac attcgccgcc gtccgcgaag
    11101 cataccgggc ggcagcctca aaagcgggcg agaacgacag aatcgtcgtt ttcggctcat
    11161 ttcataccgt tgccgatgtc atgtcggtac tttaaaagga aacttctatg tccgaaaaca
    11221 aacaaaacga agtcctgagc ggttacgaac aactcaaacg gcgcaaccgc cgccgcctcg
    11281 taacggcaag ttgcctggtt gccgcctcct gcatcctgct ggcagccgcc ctcagttccg
    11341 gccctgccga acagactgcc ggcgaaacaa gcggcgtaga aaacaaagcg gcaggtgcgg
    11401 cacaaacccc tgccttgaaa tccgccgccg acaaaccgca ggacttggca ggcgaagaca
    11461 agccttctgc cgccgacagc gaaatcagcg agcctgaaaa cgtaggcgcg ccgctggtgc
    11521 tgattaacga ccgcctcgaa gacagcaaca tcaaaggttt ggaagcatcc gagaaactgc
    11581 aacaggcaga aaccgccaaa accgcaccga agcaggcaaa acaacgcgct gccgaaaaag
    11641 tgccggcaac tgccgacagt acggatacgg tagcggttga aaaaccgaaa cgcactgccg
    11701 aaacaaaacc gcaaaaagcg gaacgcactg ccaaagccaa gcccaaagcc aaagaaacca
    11761 aaaccgccga aaaagttgcc gacaaaccga aaactgccgc cgaaaaaacc aaaccggata
    11821 cggcaaaatc cgacagcgcg gtaaaagaag cgaaaaaagc cgacaaggct gaaagcagaa
    11881 aaacagccga aaaagaccgt tcggacggca aaaaacacga aacggcacaa aaaaccgaca
    11941 aagcggacaa gaccaaaacc gccgagaagg aaaaatccgg taaaaaagcc gccattcagg
    12001 caggttatgc cgaaaaagaa cgcgccttaa gcctccagcg caaaatgaag gcggtgggta
    12061 tcgattcgac catcaccgaa attatgaccg acaacggcaa agtttaccgc gtcaaatcaa
    12121 gcaactataa aaacgcaagg gatgccgaac gcgatttgaa caaattgcgt gtacacggta
    12181 tcgccggtca ggtaacgaat gaataacctc cccgttgccg acctcctcgt cttcgccatc
    12241 atcgccgcct gcatcgtgct atccgcgatg cgcggcgtga ttgcggaggc aggttcgatg
    12301 gcggcatggg tggtttcctt cttttttgcc aaactctttg ccgcaccctt cgccgacctc
    12361 gcctttgcct cgttccaacc ccgcctgttt gcattggctc tgtcgttcat ttccctgttc
    12421 gtcattgcct gtctgatcca gaaaatgctc cgttcgctgc tgaccggcgc agtttcggcg
    12481 gtcggtttgg gctttgccaa ccgcattttg ggcggcgtat tcggtgcatt gaaaggcgtt
    12541 ttgattgtta ccctgctggt catgcttgct tcaaaaaccg acctgcccga taccgaagag
    12601 tggcagcagt cctataccat accgtttttc gtatcgcttt ccgaagccgt gttgaaccat
    12661 agcggcggca cggcggaaac tccggaagac gattgattgc catacggata tttcagacgg
    12721 cattctcttt tatgccgtct gaaatgtttt ttccttttct cagccatatc acagattaaa
    12781 tcttatcctc cataaaccca cctatctgtc cttcgctcaa atattgtcaa caattcaggc
    12841 aaaaaaccac cgtttgcttt ataattccgt tttaccctta aacctcgaat cggagaagac
    12901 gatatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac
    12961 ggtttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc
    13021 ggcaccttcc atatgcacaa aggcaaaggg atggtgcgcg aagtgttccg cacgcgcaat
    13081 atgcgcgatt tggtcggcaa cgccggcatc gcccacgtcc gttatcctac ggcgggcaac
    13141 gccggcagca gcgcggaggc gcagcctttc tacgtcagct cgcctttcgg catcgttttg
    13201 gcgcataacg gcaatctcac caacacggcc gaactgtatg aaaacgtgtg caacaaacac
    13261 ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc tcaacgtatt cgcgcacgaa
    13321 ttacgccgcg aagtctccaa aaacgccgat ccgcaccggc tcaatgccga caatattttc
    13381 aacgccgttg ccgaagtcca ccgcctagtg cgcggcgcat acggcgtggt tgccatgatt
    13441 gcgggctacg gtatgcttgc cttccgcgac ccttacggca tccgcccgct ggtattgggt
    13501 tcgcaaaccg acaacgaagg caggaaatcc tatgccgtcg cctccgaatc cgtcgccttc
    13561 aacgcgcttg cctacgattt ggaacgcgac atccggccgg gcgaagcggt atttgtcggc
    13621 tttgacggca caatgattgc ccgccaatgc agcgaccgtg ccaaactcag cccctgcctt
    13681 tttgaatatg tttattttgc ccgccccgac tccgtaatcg acggcgtatc ggtttaccaa
    13741 tcgcgcttgg atatgggcgt gtccttggcg gaaaaaatca aacgcgagct gcccgtggac
    13801 gacatcgatg tcgtgatgcc cattcccgac accagccgcc ccagcgcgat ggagcttgcc
    13861 gtccacctcg acaaacccta ccgcgagggc ttgattaaaa accgctatat cggccgcacc
    13921 tttattatgc ccggccaggc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg
    13981 gaaaccgagt ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcgggacg
    14041 accagccgcg aaatcgtcga aatggtacgc gcggcgggcg cgcgcaaagt ctatatcgcc
    14101 tccgccgccc ccgaagtgcg ctatcccaac gtatacggca tcgatatgcc cacgcgcgaa
    14161 gagttgattg ccaacgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc
    14221 atcgtgttcc aaaatttgag cgatttggaa gccgtcgtca aagcactcaa tccgaaaatc
    14281 gaatccttcg attcgtcctg tttcaacggc atttatcaga ccggcgacat cgatgatgcc
    14341 tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg gcctgaaaat ccacccgagt
    14401 aggatggaac acagcatcag catcagcgat gcgggcgacg aagaataagg cttcccaaac
    14461 cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg gcatttccca tatcggcatc
    14521 acatttccca tttcatccgc acgcaatatg agtaccgaaa ccaaaaacta cattacgccc
    14581 gccggatggc aggcattgaa agacgagctt tatcaactgg ttaataaaga acgccccgaa
    14641 atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc gcagcgaaaa cggcgactat
    14701 ctttacggca aacgccgtat gcgcgaaatc gaccgccgca tccgtttcct gaccaagcgt
    14761 ttggagtccg ccgtcgtcgt cgatcccgaa ttgcgcgaag cgaccgacca agtatttttc
    14821 ggcgcgacgg tcgggctgtt gcgcggcgac gggcgcgagc aaaccgtcaa aattgtcggc
    14881 atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga tttcgccgtt ggcgcgttgc
    14941 ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca cgccggaagg gcgtgaggaa
    15001 attgaaatcc tttctgtcga atacatcaaa atcgactgat cgggaatgcc gtctgaaacg
    15061 gcacaaacgc atcgtttccc aaataaagga ataccgaatg aggaaaatcc gcaccaaaat
    15121 ctgcggcatc accacactgg aagacgcagc ggccgccgca gcggcaggtg cggatgccgt
    15181 cgggctggtc tttttccaag gcagcagccg ggccgtcgat attgcccgcg ccaaaaaaat
    15241 caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt ttcgtcaacg aaagcgcgca
    15301 aaacatccgc cgcatccttg ccgaagtgcc gatacacatc atccaattcc acggcgacga
    15361 agacgacgca ttctgccgcc agttcgaccg cccctatatc aaggccattc gtgttcagac
    15421 ggcatcagac atccgaaacg ccgccgaccg cttccccgac gctcaggcac tgctgttcga
    15481 tgcctaccac ccttcggaat acggcggcac cggacaccgc ttcgactgga cgctgctggc
    15541 ggaatattcg ggcaaaccgt gggtgcttgc cggcgggctg acccctgaaa acgtcggcga
    15601 agccgtccgc atcaccggag cggaagcggt cgatgtatcc ggcggcgtgg aagcgtctaa
    15661 aggcaaaaaa gacccagcca aagttgccgc ctttatcgca accgccaacc gcctatcccg
    15721 ttaaagcaac aaaaattgcc gccggaatga cttatagtgg attaacaaaa accagtacgg
    15781 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
    15841 cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    15901 cactataatc tcaaaaattt atgccattga atcaataatt tctgatgaat tttga
//
LOCUS       DAXIHS010000045        15694 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXIHS010000045 DAXIHS010000000
VERSION     DAXIHS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.045"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..167)
                     /locus_tag="ACFOF2_001237"
     CDS             complement(<1..167)
                     /locus_tag="ACFOF2_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC7107557.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYP"
     gene            complement(318..710)
                     /gene="rpsI"
                     /locus_tag="ACFOF2_001238"
     CDS             complement(318..710)
                     /gene="rpsI"
                     /locus_tag="ACFOF2_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC7107558.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(720..1151)
                     /gene="rplM"
                     /locus_tag="ACFOF2_001239"
     CDS             complement(720..1151)
                     /gene="rplM"
                     /locus_tag="ACFOF2_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC7107559.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1329..1508)
                     /gene="ssrS"
                     /locus_tag="ACFOF2_001240"
     ncRNA           complement(1329..1508)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFOF2_001240"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1514..1819)
                     /locus_tag="ACFOF2_001241"
     CDS             complement(1514..1819)
                     /locus_tag="ACFOF2_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC7107560.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMATLNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1830..2159)
                     /locus_tag="ACFOF2_001242"
     CDS             complement(1830..2159)
                     /locus_tag="ACFOF2_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107561.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(2214..3203)
                     /locus_tag="ACFOF2_001243"
     CDS             complement(2214..3203)
                     /locus_tag="ACFOF2_001243"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC7107562.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEERE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3326..6028)
                     /gene="ppc"
                     /locus_tag="ACFOF2_001244"
     CDS             complement(3326..6028)
                     /gene="ppc"
                     /locus_tag="ACFOF2_001244"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC7107563.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            6071..6964
                     /locus_tag="ACFOF2_001245"
     CDS             6071..6964
                     /locus_tag="ACFOF2_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC7107564.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINYNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVHFEGQLAVYQPDFPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            complement(7662..7886)
                     /locus_tag="ACFOF2_001246"
     CDS             complement(7662..7886)
                     /locus_tag="ACFOF2_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC7107565.1"
                     /translation="MDGLEGLECRIVELEIQSALQEDVIVGLNAMVAELRQTLDLQQA
                     QLRLLYGKLQDKNSDAQEPYSLRDEIPPHY"
     gene            complement(7887..9275)
                     /locus_tag="ACFOF2_001247"
     CDS             complement(7887..9275)
                     /locus_tag="ACFOF2_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC7107566.1"
                     /translation="MNAVVIAVIVMLVLSLSRVHVVLSLTVGAFVGGVVAGMPLQNIT
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAIIRKLNR
                     SSMPDSVRSGEGAVKWLLLSIILVMGIMSQNVVPIHIAFIPMIVPPLLLVFNRLKVDR
                     RLVACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPELDVKNINVMAAMAIPAL
                     GMVIGLLLSFVHYRKPRLYQSNDTDAAGNADAANQPQASSYRSLVAAIAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRNKASDVFGEGIKMMAMVGFIMIAAQGFAAVMSA
                     TGHIQPLVESSMAMFGGNKGMAALAMLLVGLLVTMGIGSSFSTLPIIAAIYVPLCISL
                     GFSPLATVSIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LMIAGWIAAMVL"
     gene            complement(9376..10197)
                     /gene="prmC"
                     /locus_tag="ACFOF2_001248"
     CDS             complement(9376..10197)
                     /gene="prmC"
                     /locus_tag="ACFOF2_001248"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC7107567.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDVIVSNPPYIENGDIHLSQGDLRFEPKNALTDFSDGMSCIRALAEGAP
                     KYLADGGFLLLEHGFNQGGAVRGVLAAHGFTEVKTLTDLAGLDRVTLGKHMKHLK"
     gene            complement(10712..12196)
                     /gene="tldD"
                     /locus_tag="ACFOF2_001249"
     CDS             complement(10712..12196)
                     /gene="tldD"
                     /locus_tag="ACFOF2_001249"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC7107568.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVRERLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGQRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      12424..12527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12594..13820
                     /gene="cytX"
                     /locus_tag="ACFOF2_001250"
     CDS             12594..13820
                     /gene="cytX"
                     /locus_tag="ACFOF2_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC7107569.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATL
                     TATLAYTLTSCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDA
                     YSAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVL
                     VRLFFKKTQSLQRNPS"
     gene            13817..14917
                     /locus_tag="ACFOF2_001251"
     CDS             13817..14917
                     /locus_tag="ACFOF2_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC7107570.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRGIRCRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRTDDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTVWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            14938..15555
                     /gene="thiE"
                     /locus_tag="ACFOF2_001252"
     CDS             14938..15555
                     /gene="thiE"
                     /locus_tag="ACFOF2_001252"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC7107571.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3309 a         4381 c         4324 g         3680 t
ORIGIN      
        1 ggataggcgg gacgaccgcg gtggtctcgg aggtaacggg ttctttgacg gttcaggtat
       61 tgttcgatcg gttgccaatc aatcacttga tctagcttca atagtgggaa gcggtcgatg
      121 tgtttggcaa tcatggcttg tgcggtttgc tgaaagaagg tgctcatgga aaatccccta
      181 aatggcttgg tgggaattta ggggattttg gggaattttg caaaggtctc aatctgtaat
      241 ctttagattg ccaatgggaa accgtctact acaaataaaa aaccctgcga taagcagggt
      301 tttttgaatt tccaacatta acgtttggag aattgttttg cacggcgtgc tttgcgcaga
      361 cccggttttt tacgttcaac ttcgcgggca tcgcgggtaa caaaaccagc ttgagacaag
      421 gcgggtttca acgcggcatc gaagtcgatc agggcacggg taatgccgtg gcggattgcg
      481 ccggactggc cggtttcgcc gccgccaaca acattgactt tgatgtcgaa agattcggcg
      541 ttttcagtca gaaccaaggg ttggcgaaca accattcggc tggtttcgcg tgcgaagaat
      601 tcgtcaacgg gacggccgtt tacgatgatt tgacctgtac ctttaatcag gaatacacga
      661 gccactgaac ttttgcggcg gcctgtgccg tagtagtatt taccgttcat gtcgcgtcct
      721 tatttcagtt ccaaaacttt gggttgttgc gcagcatggg cgtgttccgc acccgcatac
      781 actttcagtt ttttaatcat ggcgtaaccc aaaggacctt tgggcagcat accttttaca
      841 gcttgttcca aagcgcggcc cgggaattgc tcttgcattt cgcggaaagt gcgttcgtag
      901 ataccgcctg ggaaaccgga atggcggaag tattttttat cttcgaattt ggcaccggtt
      961 acacgcagtt tgtccgcatt gataacaatg atgtaatcgc cggtatcgac gtggggggtg
     1021 tattcaggtt tgtgtttgcc acgcagacgg ctggcgactt cggccgcaac gcgacccaag
     1081 actttgtctt gggcatcgat gacgaaccat tcgcgcttca cctcgtgggg tttcgctgaa
     1141 aaggttttca tagtggaaat ccagatagat atagaaagtt gtaaatttta aagacaggat
     1201 tcgattttgt caatcgcatt accgcgttac ggaaggattt tccggatttc ggcagactgc
     1261 atactgcttt ttcgggcggg gcggcggcca atgtgaaaaa ccgcatcgtt gcgatgcggt
     1321 tttgaatggg aatccccgcg agagccgttt cggccgaatc cgcttgaacc ttgctgacaa
     1381 ggcggctgcc tcgggtagtt tcgggtgcgt ccgcaaaagg acgctcgcgc ccactactgc
     1441 tcccggcaac cttaagcgaa cttattggtt caaaggaata tatgccttcg cggacaccgc
     1501 agggaaaaag gggttattcc tgcgccaagc gggatagtgc tttttggcag gcgttgtcca
     1561 tatcggctat tttacgcgca aaatcgccga ttgccaaatc gccgccgttc agggaggttt
     1621 tcaacaggtc gtggacgacg ttgagcgtgg ccataatgac gattttttcg ctgtccgcga
     1681 cgcgtccgcc ttcgcggatg gcttcggctt tgccgttgag cattccgact gcctgcaaca
     1741 gtgtgtcttt ttcttctgcc ggcgtgttga cggtcagccg ggcgtgcatg acttcgatgt
     1801 ggacttgttc gatgttcatc ctttaatcct tattgctgcg tttcctgcca ttgggggagg
     1861 cgcgctgcca gtgcgctgat tttttccctg ctctgttcga gcaggctgcg gtatcgtgta
     1921 ttttcttctg tcaggctgtc gattttgttt tgcaggtctt ctttgagttt gccgacttgg
     1981 acgagcaggg cttcgctgag ttcgtcgacg gcggtttcgt gttcgagttt ttgccgctcg
     2041 tgcgcccgtt tgagttcggc gacggtttct ttgaggcggc ggttttcgct gacgagggtt
     2101 tcgaattttt gtaccagcgt ataaacgctg ctttcgagtt tttcgatatt ttgtttcata
     2161 accttacctg tccgtatgcc gtctgaaggc ctcagacggc atctgtctgt tgtttattcg
     2221 aaacgcgcgc tgcgttccat cagtctttcg acgacctgct gaggggtcat ttctttgcgg
     2281 atgagttgca gcagggtttg ggtaatcggc atgtcgattt ggtacttaca ggcagtattg
     2341 aagacttctt ctatcgtgct gaccccttcg gaaacgtgtc cgatttcgac cagcacctga
     2401 tgcagttcct tgccttctgc caaacccaag ccgacgcggc ggttgcgtga gagtgcgccg
     2461 gtgcaggtca agatgaggtc gccgatgcca gcaagcccca tcatggtttt gggctgtgcg
     2521 cccattgcgg aggcaaggcg ggtgatttcg gccagtccgc gtgtaaccag tgcggcacgg
     2581 gcgttaagcc cgtactccag accgtcggac aatccggtgg caatcgccat gacgtttttg
     2641 actgcaccgc cgactgccac gccgataacg tcggtactgc cgtaaagcct catgacggtc
     2701 gtgttgagct gcggtacgag ttcttcaatc cactcttggt tttcggaggc gaggacgacg
     2761 gcgcagggca gttgtttggc gagttcctgt gcaaaactcg gaccggaaag tacgccgatt
     2821 tttttattgt cgggcaatac ttctttcaat acttgaaagg tcagcagccc tgtatcctgc
     2881 tcgaatcctt tgcaggcggc gaggacgggg aggtgtcccg cgccgtactg tttgagcaac
     2941 tctgcgctgc ttctcaatcc ggcaacggag gttacgataa ggacaagtcc gctgtctttg
     3001 agcgcgtctg ccaaatccgc acacacttcc aaggtttcgg gaaaggaaaa gccgggcagt
     3061 ccgcgtttgt tttcacgttc ttcctgcatt tggcggactt ggtctgcgtt gcgcgtccac
     3121 agggatacgc ggttgccgtg ttgggaaaaa tgcagggcga gcgccgtacc ccacgaacct
     3181 gcgccgataa cggtaatttt cattggtcgt ctttcaacat atcactgccg ttcactttaa
     3241 aacaatcggt gtttctctgc aagtgcggtc agggaaatgc cgtctgaaag gcgttcagac
     3301 ggcattttgc cccgatgcgg cactatcagc ctgtattgcg caaaccttgc gccacgccgt
     3361 tgatggtcag gtgcaccatc agaagggcgt gcggattgtc gggttcttta cgcaggcgtt
     3421 tgagcatggc gacttgcagg ccgttgaggg cgttcaaata tggaatcctc aaggcaagcg
     3481 aacgggcgag gctgcggttg tcgcgcagga gctcttcggt ttgcagcaag tcgagcagtg
     3541 ctttgcggct gcgttggtat tcttccttga tcatgccgaa gatgactttt gctttgtcgg
     3601 gtgattcgct caagccggca tagttttccg ccaaggtgat gtcggttttc gccatcactt
     3661 gttccatatt ggagagcatg gcttggaaga acgggttgct ttgggcgtgt tcgcgcaggg
     3721 cggcgagcgt ttcgggtttg tcttcgcaca aggtttccac cgcgctgccg aaaccgtacc
     3781 aagcaggcag catgaggcgg ttttgcatcc aggaaaatac ccacggaatc gcgcgcaagt
     3841 cttgaatccg cgctaaggtc ttgcggctgg cgggacggct gccgaggttg agggtggcga
     3901 tttcctgaat cgggctggtt tgcaggaagt agtcgatgaa gtcggggtgg gtaatcagtt
     3961 cgcggtagta tttgaacgat acgtccgaca atgcctgcat cagtttggca tcagggtctt
     4021 ttttatccgg caggatgctg gcttccaaag tcgcggcaac caaggtttcc aagttgcgtt
     4081 gggcattgcc ggggtcggcg tatttggcgg taatgacttc gccttgttcg gtaatgcgga
     4141 tttgtcccgc cacgctgccc gccggttggg cgagaatggc ttggtaagaa gggccgccgc
     4201 cgcgtccgac gctgccgccg cgaccgtgga acaggcgcat gcggacatcg tattttttga
     4261 agagttcgac caagcccaat tccgcctgat acagacacca tgagctggta acatagccgc
     4321 cgtctttgtt ggagtcggag tagccgagca taatttcttg gatgttgccg cagctttcga
     4381 gcagggcatc gtaccaatcg aggcggaaca tggtttccat gaccggacag gcgttttcga
     4441 gtgcttcgat ggtttcaaac agcggcacga tattgatgcg gctgcgcggt ttgccgtttt
     4501 ccaccgccag caggccgctt tctttcagca gcaatgccaa ggcgagcagg tcgctgggtt
     4561 gttcgcagtt ggaaataatg ctttgtgtaa cggcatcttc gccgaattcg tctttgattt
     4621 tgcgcgcttc gttgaaaatt gccagttcgt ggcgggtatg gtcgctgtat gtgatgaacg
     4681 ggctgtacag cggacgttga tggctcaatt cgcgcaacag ggcggcttgt ttttgctctt
     4741 cgttcaggct gttgtagtct tccaagcctg cgtgttggaa aagctcggca accacatcgg
     4801 cgtgtttgcc tgcgtgttgg cgcaggtcga gcggcatcat gtggaagccg aacacggaca
     4861 cggaacggat gaggtctgcc aaacggcctt cagcaagcag gcggctgccg ttgtcgataa
     4921 gggaacgttg caattttttc aaatcatcga gaaattcttg tgccgaagca tacggctcga
     4981 gaaagccgaa tttgcagccc atgcccaaac cgagcgcgcg cgctttgccc atagcgcgtg
     5041 ccatgatgta ggcgatggcg cggcggtagg gttcttcggt acgggcgatt tcttcgtcag
     5101 gcgagagggc ggccagtgcc attacgtcgt cgttgacttt gacgcggcgg atggagagcg
     5161 gcagttcgcg gtaaagtttg tcgagttcgc cacggtaaaa acggaacacg gcatcggcgt
     5221 ggcggcggaa ggcaaaacgc aaggtttcgg cagaaacgaa cggattgccg tcgcggtcgc
     5281 cgccaatcca gccgccgatt ttgaggatat tcggaacgca gacatcggga tagaccatct
     5341 gaaagtcgtg ttccatcttg cggtagagct tgggcagggc ttcgaaaaag ctcatcggga
     5401 agatggacac gccgttgttg atttcgtcgt tgacgctgag tttgtggcgg cgcgtttcgc
     5461 tggtctgcca caagcccagc aggatagtgt cgatttcgcg gcgcagccgt gccagcgcgt
     5521 cggcattggt gcagcgttcg cgttgcggca acagtgcgcg gatgcggcgg ttgaagctta
     5581 agacggtttg gcgttgcact tcggtcgggt gcgcggtcaa aacggcggta acggacgtat
     5641 tgtccaactg ccgctgcacc gatttgccgt cggctttccc cgctttgagc ctgcggacgg
     5701 tttccgtcag gctgccttcc gcgccgccgc gtccggcttc ttcgtggatt tggcggcggc
     5761 gttcgtggtg cacgtcttcg gcgatgttca aaatctgtgc gaacaggccg caggccaagg
     5821 ttaaatcgtg ggtttgttgt tcgtccaatt gcggcaatac tttttcaatc aatgccgcgc
     5881 tgtcgtcgga agtggacaag agtttgaccg tttcgacaac caatggcgag gcttcttcgt
     5941 gcaggaggtt gaacagggat tgtttcagaa attccgcgtc cgccgccaaa gccgcgtcct
     6001 ttggattgtt cagaatatgc agttgcatga tttttctctc attgccgtaa atactgtaaa
     6061 tgtacctcaa atgccgcatc cgtgccaaac cgttcacact ttaaccgccc gtgtcccgaa
     6121 atgccgtctg aagttgaacg ccgcccgacg gcagcgttac aatcgcccac aactgttttt
     6181 tccgaacatc atcatgacca cgaccgaaca cgacaacgac gatgcattcc tgctgcggta
     6241 cagccgccac atcctcttgg acgaaattgg catcgaaggg cagcagaaac tttccgccgc
     6301 gcatattttg gtcgtcggct gcggcggttt gggtgccgcc gccctgccct atctcgccgc
     6361 ttccggcatc ggcacgctga ccatagccga ttccgacacg gtcgagctgc acaacctgca
     6421 acgccaagtc gcatttgacg agggcgatgt cggcaagccc aaagccgaag ccttggcaga
     6481 ccgcctgaaa cgcatcaact ataacgtcga tgtccgcacc gtcaacgaaa aactcgacgg
     6541 ctgccgcctg accggtttgg ttcaaaccgc cgacatcgtt ttagactgct gcgacaacta
     6601 cgccacgcgc caagccgtca accgtgcctg cgtacaaacg aaaacaccgc tggtttcagg
     6661 agcagcggtg cactttgaag ggcagcttgc cgtgtaccaa cccgactttc ccgactcgcc
     6721 gtgttacgcc tgcctgttcg acggcggatc ggcttcagac ggcatctgtt cccttttcgg
     6781 cgtattctcg ccgctggtcg gcatcatcgg ctgcacgcag gcggcggagg cactgaaaat
     6841 cctgctggat gcaggcgaac cgtcgcacgg caggctggcg gtttaccgtg ctttggaagg
     6901 gggctggcaa tatttcgacc taccgcgcaa ccctgaatgc ccggtttgcg gcgcggcacg
     6961 ataaaccgtg ccgccgtttc agacggcatc taaacggatt cggcggaacg gttttaaaaa
     7021 tcggcggtgt tttgacatag tgtcgataag catacttatc ttcaatatcc tcaacaggat
     7081 aattgtttgc ctaagcaaaa aaataatcgc cttttccctg acggtcgggc gaaatccacg
     7141 gattgcaaaa acacaacctg aaaatttaca tttctttgca aaaaaaaaaa aaaaaaaata
     7201 aacttacctt ataattgcaa ttgttttagc aatatctgat tcgcagattc attgagttaa
     7261 gatattttcc ccgtaatgtg tttggccgtc tgtccctttt gggttcggac ggcttttttt
     7321 aggctgtgtt tgaatacccg gttgatttcg tctgtttgta gggggaaggc cgcttatttc
     7381 cgttcgggta gaaacgctcc catcggataa aaggcatttt gtccgactga ttaaagttat
     7441 agtggattaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag aacgattctc
     7501 taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt
     7561 cgccttgtcc tgatttttgt taatccacta tatatcttag gtttgcatcg acggaatatt
     7621 caaacacagc ttttaggggg aatccggatg ccgacggcgc atcaataatg cggcgggatt
     7681 tcgtcgcgca gggaatacgg ctcttgcgcg tcggaatttt tgtcttgcag cttgccgtac
     7741 aacagcctca actgagcctg ctgcaaatcc aacgtctgcc gcaattccgc caccatcgca
     7801 ttcaggccga cgattacgtc ttcctgaagc gcggattgga tttccaattc gacaatacgg
     7861 cactccagcc cttcaagccc atccatttac agcaccatcg cggcaatcca accggcaatc
     7921 atcagcggga tgttgtagtg gatgaaggtc gggatgacgg aatcccggat atggtcgtgt
     7981 tgtccgtcag cgttcaagcc catggtcggg cccagcgtcg aatcggacgc gggcgaaccg
     8041 gcatcgccca acgcacccgc cgtaccgaca atagagactg tggcaagcgg cgaaaagccc
     8101 agactgatgc acaacggtac ataaatcgcg gcaataatgg gcaaagtgga aaaggacgaa
     8161 ccgataccca tggttaccaa aagccccacc agcagcatgg ccaatgccgc catacctttg
     8221 ttgccgccga acatcgccat gctactttcg accagcggct ggatgtgtcc ggtcgcactc
     8281 ataacggcgg caaaaccctg tgccgcaatc atgataaaac cgaccatggc catcatcttg
     8341 atgccttccc cgaagacgtc gctggccttg ttgcggttaa ttacgcccaa catcataaat
     8401 acggcgaaac cgagcatcgc ccccaacacc agcgaatctt cgtacatcag ctggatggca
     8461 aaacatacgg caatggcgat ggcagcaacc aggctgcggt atgaagacgc ctgaggttgg
     8521 tttgccgcat cggcattgcc cgccgcatcg gtatcattgc tttgatacag gcggggcttg
     8581 cggtaatgta caaacgacag caggagtccg attaccatac ccaatgcggg gatagccatt
     8641 gccgccatca cgttgatgtt tttaacgtcg agttccggcg cggcggaatg gatgttgccc
     8701 aacagaattt catttaagaa aatcgcgccg aaaccgtaag gcaggaacat ataggtcgta
     8761 accagaccaa aagtgatgac gcacgcaacc agtcggcggt cgaccttcag gcggttgaac
     8821 accaaaagca gcgggggaac aatcatcgga ataaaggcaa tatggatagg cacgacgttc
     8881 tggctcatga tgcccatcac caaaataata gaaagcagca gccatttgac cgcgccttct
     8941 cccgaacgta cgctgtcggg catactgctc cggttcaatt tgcggataat cgcacccgca
     9001 agctgttggg gcagtcccga atgggtgatt gccatggcaa acgcaccgag catggcataa
     9061 gagagcgcaa ttttcgcgcc gccttccaag cctttgttaa atacggggat aatccccgcc
     9121 tgactgacct gtccggccgc atcagttata ttttgcagcg gcatacctgc caccacgccg
     9181 ccgacaaatg caccgaccgt caaactcaat actacatgca cgcgcgacag cgacagcacc
     9241 aacatgacga ttacggcgat tacgactgca ttcatcttcg ttcctcctta ttttttctcc
     9301 aaaaattaaa ataaaattta catatcgaaa cctatcataa accaataatg gaaaaactgc
     9361 caattttgta aacacttatt tcaaatgctt catatgtttc cccaacgtaa ccctgtccaa
     9421 cccggccagg tcggtcaagg ttttcacttc cgtaaaacca tgggcagcca acacgccgcg
     9481 caccgcaccg ccctgattga atccgtgttc caacagcaga aaccctccgt ctgccagata
     9541 cttgggtgca ccctccgcca atgccctgat gcagctcatg ccgtctgaaa aatcggtcag
     9601 cgcgtttttc ggctcaaacc gcaaatcccc ttgagaaaga tgaatgtcgc cgttttcgat
     9661 ataaggcggg ttggaaacga tgacatccca ttgcctttca gacggcatat cggtgtcgaa
     9721 ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt tccgcgccgt
     9781 ttcaagggcg ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc gttcgagcgc
     9841 gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc cgttttcggg
     9901 caggcgcgcc aatacggctt cgaccaagtg ttcggtttcg gggcgcggaa tcagcacgct
     9961 cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat aggcaaccgg
    10021 ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt cgtccggcat
    10081 ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat attgcagcag
    10141 cattcttgct tcatttttag gcagttttga caagcccaac catttatcaa acgtcatttt
    10201 tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa acgtaccgat
    10261 ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc gcacaatatc
    10321 cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca ccaacgccaa
    10381 gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca aaacaatggc
    10441 cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag aagcaaatat
    10501 cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca aataaaatgc
    10561 aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca ctatgggaaa
    10621 acttgatttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc cgcctgaaaa
    10681 cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg acggtcagcc
    10741 cggcatcgat acgcagggtc ggctgcccca cgccgacggg gacgctctgc ccttctttgc
    10801 cgcacacgcc gacaccgctg tccaacgcgg tatcgttgcc tatcatcgaa acgtgtttca
    10861 gcacttcggg cccgttgccg ataatggtcg cgcctttgac ggggtattgc agcctgccgc
    10921 cttccaccca ccatgcttcg gacgcgctga acacaaattt gccgctggta atgtccacct
    10981 gtccgccgcc aaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg atttcttccg
    11041 gctcatagct gccattttcc ataaaggtat tggtcatgcg cggcatcggg acggaggcgt
    11101 agctttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca ttggtttcgt
    11161 cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg gtttcgttgc
    11221 cttcgtcgtc gatgttgagc gaaccgcgct ggccggcaat atcgccctga tcgacgacgg
    11281 taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg gtccccttgc
    11341 ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg cccggccagc
    11401 cgttgcccaa aacgacggtc atttcgccag cgggcgcggg gcgggattcg aggttggtga
    11461 gtgcctgttt gacggcggca tcgacaaact gccgcaccaa gttttcatcg aaataagcca
    11521 agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc tgtttggcga
    11581 taacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg ccgtccagac
    11641 gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc acgatgcgcg
    11701 gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg gcggcggaat
    11761 cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt tcagacggca
    11821 ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg gcggaacggt
    11881 ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttatcg cccgaaacgg
    11941 cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg ccctcttcca
    12001 aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcataatcg acgtggtgcg
    12061 cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg gcttcgagca
    12121 gccgctctcg tacggcggaa taggtaggat gcatagtgtc ggcgcataaa aaatcagggg
    12181 cttgattata cggcatttgt tatatagtgg attaacaaaa accagtacgg cgttgcctcg
    12241 ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttccgta
    12301 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatagaa
    12361 atgcgccgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt gtgtaaactt
    12421 aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc agaatccgta
    12481 gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta tcgggcttcc
    12541 gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt catatgtcgg
    12601 gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc ggcgcggcgg
    12661 tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg caacgcggtc
    12721 tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
    12781 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
    12841 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
    12901 tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
    12961 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt tttggcgcac
    13021 gcagaacggg cgggctgaaa accgtttcga tgctgctgat gctgcttgcc gtgttgtggt
    13081 tgagcgtcga agtgttcgct tcgtccggca caaacgcagc ccccgccgtt tcagacggca
    13141 tgagcttcgg aacggcagtc gaactgtccg ccgtcatgcc gctttcctgg ctgccgcttg
    13201 ccgccgacta cacgcgccac gctcgccgtc cgtttgcggc aaccctgacg gcaacgctcg
    13261 cctacacgct gacgagctgc tggatgtatg ccttgggttt ggcagcggct ctgtttaccg
    13321 gagaaaccga tgtggcaaaa atcctgctgg gcgcaggttt gggtgcggca ggcattttgg
    13381 cggtcgtcct gtcgaccgtt accaccactt ttctcgatgc ctactccgcc ggcgtaagtg
    13441 ccaacaatat ttccgccaaa ctttcggaaa cacccatcgc cgttgccgtc gccgttgtcg
    13501 gcacactgct tgccgtcctc ctgcccgtta ccgaatatga aaacttcctg ctgcttatcg
    13561 gctcggtatt tgcgccgatg gcggcggttt tgattgccga ctttttcgtc ttgaaacggc
    13621 gtgaggagat tgaaggcttt gactttgccg gactggttct gtggctggcg ggcttcatcc
    13681 tctaccgctt cctgctctcg tccggctggg aaagcagcat cggtctgacc gcccccgtaa
    13741 tgtctgccgt tgccattgcc accgtactgg tgcgcctttt ctttaaaaaa acccaatctt
    13801 tacaaaggaa cccgtcatga cccgtatcgc catcctcggc ggcggcctct cgggaaggct
    13861 gaccgcactg cagcttgcag aacaaggtta tcagattgca cttttcgata aaggctgccg
    13921 ccggggcgaa cacgccgccg cctatgttgc cgccgccatg ctcgcgcctg cggcggaagc
    13981 ggtcgaagcc acgcctgaag tcatcaaact gggcaggcag agcatcccgc tttggcgcgg
    14041 catccgatgc cgtctgaaaa cacctgccat gatgcaggaa aacggcagcc tgattgtgtg
    14101 gcacggacag gacaaacctt tatccaacga gttcgtccgc catctcaaac gcggcggcgt
    14161 agcggatgac gaaatcgtcc gttggcgcac cgacgacatc gccgaacgcg aaccgcaact
    14221 cggcggacgt ttttcagacg gcatctacct gccgaccgaa ggccagctcg acgggcggca
    14281 aatattgtct gcacttgccg acgctttgga cgaactgaac gtcccctgcc attgggaaca
    14341 cgaatgtgcc cccgaagacc tgcaagccca atacgactgg ctgatcgact gccgcggcta
    14401 cggcgcaaaa accgtgtgga accaatcccc cgaacacacc agcaccctgc gcggcatacg
    14461 cggcgaagtg gcgcgggttt acacacccga aatcacgctc aaccgccccg tgcgcctgct
    14521 gcacccgcgt tatccgctct acatcgcccc gaaagaaaac cacgtcttcg tcatcggcgc
    14581 gacccaaatc gaaagcgaaa gccaagcccc cgccagcgtg cgttcagggt tggaactctt
    14641 gtccgcactc tatgccgtcc accccgcctt cggcgaagcc gacatcctcg aaatcgccac
    14701 cggcctgcgc cccacgctca accaccacaa ccccgaaatc cgctacagcc gcaaacgccg
    14761 cctcatcgaa atcaacggcc ttttccgcca cggtttcatg atctcccccg ccgtaaccgc
    14821 cgccgccgtc agattggcag tggcactgtt tgacggaaaa gacgcgcccg aacgtgacga
    14881 agaaagcggt ttggcgtata tccgaagaca agattaaagc cgtctgaaag gaaccccatg
    14941 accttcccgc cactcaaatc cccgctcaaa ttctacgccg tcgtccccac cgccgattgg
    15001 gtggggcgca tggtcaaagc aggtgccgac acggtgcaac tgcgctgcaa ggccctgcac
    15061 ggcgatgaat tgaaacgcga aatcgcccgc tgtgtcgccg cctgtcaggg cagccatacg
    15121 cagcttttca tcaatgacca ctggcgcgag gcaatcgaag caggcgcgta cggcgtgcat
    15181 ctcggacaag aagacatgga caccgccgac cttgccgcca tcgccgccgc cggtttgcgc
    15241 ttgggtttga gtacgcactc cgttgccgaa ctcgaccgcg ccctgtccgt acaccctagc
    15301 tacatcgcca gcggcgcgat tttcccgacc acgaccaagc aaatgcccac cgccccgcaa
    15361 ggcttggaca aactgtgcga atacgtcaaa caggcaggcg gcacgcccgt cgtcgccatt
    15421 ggcggcatcg atttgaacaa cgcccgagcc gtactcgcca ccggcgtttc ctcactcgcc
    15481 gccgtccgcg ccgtaaccga agcggcaaat cccgaagcgg tggttaaagc gtttcaggct
    15541 ttgtgggatg gataaaaccg aaagaagaaa attcaattgc cgtgtaggca aaacttagcc
    15601 cgttatcgca aacatactta actttaaatg tggcatatca tcaaattccg tcattcccgc
    15661 gcaagcggga atccgcctta aaacttgaga aacc
//
LOCUS       DAXIHS010000046        15298 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXIHS010000046 DAXIHS010000000
VERSION     DAXIHS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.046"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            365..1519
                     /gene="obgE"
                     /locus_tag="ACFOF2_001253"
     CDS             365..1519
                     /gene="obgE"
                     /locus_tag="ACFOF2_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC7107572.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1602..2651
                     /locus_tag="ACFOF2_001254"
     CDS             1602..2651
                     /locus_tag="ACFOF2_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC7107573.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3339..4760
                     /gene="cysS"
                     /locus_tag="ACFOF2_001255"
     CDS             3339..4760
                     /gene="cysS"
                     /locus_tag="ACFOF2_001255"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC7107574.1"
                     /translation="MTTIYNTLTRQKELFAPIDPKNVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4931..5710
                     /locus_tag="ACFOF2_001256"
     CDS             4931..5710
                     /locus_tag="ACFOF2_001256"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC7107575.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5697..6041
                     /locus_tag="ACFOF2_001257"
     CDS             5697..6041
                     /locus_tag="ACFOF2_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107576.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVRTLLSVPAT
                     LLIAFTLQDLLK"
     gene            6051..6581
                     /locus_tag="ACFOF2_001258"
     CDS             6051..6581
                     /locus_tag="ACFOF2_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC7107577.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQRQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6674..7789)
                     /gene="asd"
                     /locus_tag="ACFOF2_001259"
     CDS             complement(6674..7789)
                     /gene="asd"
                     /locus_tag="ACFOF2_001259"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC7107578.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKTNF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7966..8547
                     /locus_tag="ACFOF2_001260"
     CDS             7966..8547
                     /locus_tag="ACFOF2_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC7107579.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8609..9463)
                     /gene="folD"
                     /locus_tag="ACFOF2_001261"
     CDS             complement(8609..9463)
                     /gene="folD"
                     /locus_tag="ACFOF2_001261"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC7107580.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9686..9763
                     /locus_tag="ACFOF2_001262"
     tRNA            9686..9763
                     /locus_tag="ACFOF2_001262"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9721..9723,aa:Pro,seq:tgg)
     gene            9788..9864
                     /locus_tag="ACFOF2_001263"
     tRNA            9788..9864
                     /locus_tag="ACFOF2_001263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9822..9824,aa:Arg,seq:tct)
     gene            9892..9967
                     /locus_tag="ACFOF2_001264"
     tRNA            9892..9967
                     /locus_tag="ACFOF2_001264"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9925..9927,aa:His,seq:gtg)
     gene            10103..10609
                     /locus_tag="ACFOF2_001265"
     CDS             10103..10609
                     /locus_tag="ACFOF2_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC7107581.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10606..12384
                     /locus_tag="ACFOF2_001266"
     CDS             10606..12384
                     /locus_tag="ACFOF2_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC7107582.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12497..13321
                     /locus_tag="ACFOF2_001267"
     CDS             12497..13321
                     /locus_tag="ACFOF2_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC7107583.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14084..14872)
                     /locus_tag="ACFOF2_001268"
     CDS             complement(14084..14872)
                     /locus_tag="ACFOF2_001268"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC7107584.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     AGSGDEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15086..15280)
                     /gene="thiS"
                     /locus_tag="ACFOF2_001269"
     CDS             complement(15086..15280)
                     /gene="thiS"
                     /locus_tag="ACFOF2_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC7107585.1"
                     /translation="MNIILNGEPAELHGTTVADLIAQTAPQKPFAVAVNTGFVAKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3654 a         3884 c         4235 g         3525 t
ORIGIN      
        1 ggcgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaacc
       61 gagtttatcc tagttatcta ggacagcccc aaaacttatt atatgtaacg gtttgaacgg
      121 acattccgtc gggtcggaat gtcaaaaagg cgcgattgta ccaaagaagt tggacataat
      181 tttgtttgca ggctgaagat ttgcttaaaa attcattaag atgggcggaa caaatagttt
      241 gggtacggcg tgttgaaata ccgcttatcc cttataatag cgtccgaaat tctgttcaga
      301 cggcatcaaa acacacggta atccgtctga acccccattt gaaaccaaca aacaagagca
      361 ttgaatgaaa ttcatcgacg aagcaaaaat cgaagtcgcc gcaggcaaag gcggtaatgg
      421 cgcaaccagt ttccgccgcg aaaaattcgt accgcgcggc ggtccggacg gcggcgacgg
      481 cggcaaaggc ggcagcgttt gggcagaagc cgacgaaaac accaacaccc tcgtcgaata
      541 ccgcttcgtt aaacgctacc aagccaaaaa cggcgaaaaa ggccacggtt ccgaccgtta
      601 cggcgcaggt gcggacgaca tcgtcctcaa aatgcccgtc ggcaccctta tccgcgacct
      661 cgacaccggc gaaaacgttg ccgacctcac ttatcacgga cagcgcgtct gccttgccaa
      721 aggcggcaaa ggcggcttgg gcaacatcca cttcaaatcc tccgtcaacc gcgccccaaa
      781 acaatccacg cccggcgaag aaggcgaagc ccgttccctg caactcgaac tcaaagtcct
      841 cgccgatgtc ggcttattgg gtatgcccaa cgccggcaaa tccaccctga ttaccgccgt
      901 atccgccgca cgccccaaaa tcgccaacta ccccttcacc accctgcatc caaacttagg
      961 cgtggtacgc atcgacgaaa accacagctt cgtgatggcc gacatccccg gcctgattga
     1021 aggcgcggca gaaggcgcgg gtttggggca tcgtttcctc aaacacttat cgcgcaccgg
     1081 attattgctg cacgtcgtcg atttggcgcc cttcgacgaa accgtcaacc cggccgaaga
     1141 agcacttgct atcatcaacg aattgcgcaa atacgatgaa gaactttacg gcaaaccgcg
     1201 ctggctggtg ctgaataaac tcgacatgct tgacgaagaa gaagcccaaa cgcgcactgc
     1261 cgccttcctc gaagccgtcg gctgggacta ccccaaaccc gacgaccgtt tccaatttga
     1321 catggaaacc ccgcgcctct tccaaatcag cgcgctgacc caccaaggca cgcaggaatt
     1381 ggtacaccaa atcaaccaat acctgaccga gaaaaaacgt atcgaagctg aaaaagccga
     1441 agcggagaaa gcagcggcaa atgttgaaat tatcgaacag cagcctaaaa cggatacggg
     1501 cgtgtttaag ccggagtgaa aaaagaaagg gtttcagacg atatttgttc tacaaaaggt
     1561 tgtttgaaac aattttaaag caagattgaa atggattaaa aatgtcatct aaccaatata
     1621 tccagtcgct tgagttgaca aactttaccg ttcttccaaa cgatactttt gaattttctg
     1681 aaaatctcaa tgttattgtt gccgaaaacg gttgtggcaa aacacatttg ttaaaaattt
     1741 tatacagcct gctagaagta acatctaaca ccaaaaacag attactgaaa acagaattgc
     1801 aaaaaagttt tgccgataaa ttactgaatg ttttccgtcc cgattccttg ggcaggttga
     1861 gcaaacgttt gcaggggcgc gggcgtacgg aaatcgtttt aaaactgcaa aacggcacta
     1921 cgcatagtag tctgaatttt tccagtaact catcatcgca agtcaatgtt caaagcatcg
     1981 gattgaaaga aaatgaatat actccgacac ctatcttctt acccagccgc gaattaatta
     2041 cactttgccc ttggttcact tctttgtatc aaaaccaaag catccctttc gaagaaactt
     2101 ggtttgatac ctgtatgcaa ttaaatcacc cgcttgccaa aggccccaga gaaacaaaaa
     2161 ttcgagaatt gctcgaacct attgagaacg cgatgggcgg aaaagtatct gaagaacaag
     2221 gaaggtttta tctatcgctg acaaatacgg gcggtaagat tgaagcacct ttggtagcag
     2281 agggtttgcg gaaatttgtc atgattgccc ggttaatcgc aacaggggca ttgctggata
     2341 aaggctatct attttgggat gaaccggaag ctaatctgaa tcctaaattg atcaaagtgg
     2401 ctgcccgtat tatttggtct ttatctcaac agggtattca ggtttttatt gcaacacact
     2461 ctttattctt attgagagaa ttggaacttt tgaagcttga aaataacacg gatttacctg
     2521 tacgtttttt ctcgttgatt gcttccgatg atggaacaaa agtggagcaa gggaacagta
     2581 ttaatgattt gaacacattg atattgttag atgaaaacat tatgcaggca gacaggtatt
     2641 tgaatatttg aggctaagct atgactacaa tcacagaaaa gagattaacc tttgcttttc
     2701 ctgaagatta ttacgttacc aagtatgacg aatgggaaca ttataaaatt tttcagaaca
     2761 gttgtaattt acgtaataaa attgatacaa atgaaaaggg aaagaacggg ataaatcaaa
     2821 gtgtagatga cgataatggc agctcaggtg tagatataat agcccttcat gaatcgacac
     2881 tttggctaat cgaaattaaa gattattacc gacttgggct tgagccaaat gcccaatcaa
     2941 tagatgaaaa actttctgac ctaccatatt taatcgcaag aaaaattcga gattcattgg
     3001 caggattggt ttctgctaaa tttaaagcag aaaaacagga agaaaaagat ttttctcgct
     3061 tggctttaga ttgtaacgaa ataaaaatag tccttcatat tgaaatgcct agtataagaa
     3121 gtaaacttta tccctcgtca tcagatttag caaacttatt aaaagataag tttaaattat
     3181 cagaatttac aaaaaatttt gcaaactgtt atgcagaacc aattttcaca aatatcagcc
     3241 atattaataa tccgcaactg cgtaatgttc cttggtccgt atctacagga acagaacaga
     3301 aattatcatc cgagcaacaa agattaatcc ataatcccat gaccaccatc tacaacaccc
     3361 ttacccgtca aaaagaactc ttcgccccca tcgatccgaa aaacgtgcgt atgtacgttt
     3421 gcggcatgac cgtttacgac tattgccact taggccatgc ccgtgtgatg gtggtgtttg
     3481 acatgattgc ccgctggttg cgcgagtgcg gctatccgct cacttatgta cgcaacatca
     3541 ccgacatcga cgataaaatc attgcccgtg cggctgaaaa cggcgagact atcggcgaac
     3601 tgaccgcgcg tttcattcag gctatgcacg aagatgccga tgctttgggc gtgttgcgtc
     3661 cggatattga gccgaaggca acggaaaaca ttccgcaaat gattgccatg attgaaaccc
     3721 tgattcaaaa cggcaaggca tatcctgccg caaatggcga cgtttactac gccgtgcgcg
     3781 agtttgccgc ttacggacaa ctgtcgggca aatcgttgga cgacctgcgc gcgggcgaac
     3841 gcgtggaagt ggacggtttc aaacgcgatc cgcttgattt tgtattgtgg aaagcggcga
     3901 aagcaggcga accggcgtgg gaaagcccgt ggggcaacgg ccgtccgggt tggcacattg
     3961 aatgctctgc catgagtgaa aacctgttcg gcgatacctt cgacatccac ggcggcggcg
     4021 cggatttgca gttccctcat catgaaaatg aaatcgccca aagcgtcggc gcgacggggc
     4081 atacctgcgg tcatcatcac gcgcaaaccc accacgggca aagcatcgcc agccacgtca
     4141 aatattggct gcacaacggc tttatccgcg tggacggcga aaaaatgtcc aaatcactgg
     4201 gcaacttctt caccatccgc gaagtgttga aacaatacga ccccgaagtc gtgcgcttct
     4261 tcatcctgcg cgcccactac cgcagcccgt tgaactattc cgacgcgcat ttggacgatg
     4321 caaaaggtgc gctgacccgt ctgtacacca ccttgaaaaa cacgccggcg gctgagtttg
     4381 atttatccga aaacgccaac ggctacaccc gccgcttcta cgccgccatg aacgacgatt
     4441 tcggtacggt cgaagcggtt gccgtgttgt tcgaactggc aggcgaagtg aacaaaacca
     4501 atgacgcaca gctcgccggc tgcctgaaag ccttgggcgg catcatcggc ctgctgcaac
     4561 gcgacccgac cgagttcctg caaggcagcg cggcttcaga cggcctctcc aacgaagaaa
     4621 tcgaagactt gatcgcccgg cggaaacagg cgcgcgccga taaaaactgg gcggaatccg
     4681 accgcatccg cgaccttctg aacgaacaca aaatcatttt ggaagacaac gccggcggca
     4741 cgacttggcg gcgcggttga actgtatcgg aaagggcaaa agtaccgcct tttgctcttt
     4801 ttccgttttc agacggcatt tcccgcttga agccccgccg ttttcagcag gcagggttga
     4861 atgggaaatg ccgggcggcg caccaggtgc cgttttgtgt atgggaacat tgtgagaact
     4921 tatttttatt atgcttaaaa tcatttccgc caacgtcaac ggcatccgct ccgcctacaa
     4981 aaaaggtttt tacgaataca tcgccgcatc gggcgcggac attgtctgcg tgcaggaact
     5041 caaagcgcag gaggctgatt tgtctgccga tatgaaaaat ccgcacggga tgcacggcca
     5101 ttggcattgt gccgagaagc gcggttacag cggcgtggcg gtgtacagca aacgcaaacc
     5161 cgacaatgtg caaatcggta tgggcattga agaattcgac cgcgaagggc ggtttgtgcg
     5221 ttgcgatttc ggcaggttga gcgttatttc gctttacttg cccagcggca gcagcgcgga
     5281 agaacgccag caggtgaaat accgtttcct cgatgcgttt taccctatgc tcgaagcgat
     5341 gaaaaacgaa gggcgcgaca ttgtcgtttg cggtgactgg aacatcgccc accaaaacat
     5401 cgacctgaaa aactggaaag gcaaccagaa aaattcaggt ttcctgcctg aagagcgcga
     5461 gtggataggc aaggttatcc acaagctcgg ttggacggat atgtggcgca cgctttatcc
     5521 cgatgtgccg ggctacactt ggtggagcaa ccgagggcag gcgtatgcga aagatgtcgg
     5581 gtggcgcatc gattatcaga tggttacgcc cgaacttgcc gccaaagccg tgtccgcaca
     5641 cgtttataaa gatgaaaaat tttccgacca tgcgccgctg gtcgtggagt atgactatgc
     5701 tgccgaataa agttttgggt aaatatgatt ggaatgtgga cggaaaaaca ggaataggcg
     5761 cggcatgggt tgtcgctgcg tttatcctgc ccatattggt gtgggcgata tttatgttgt
     5821 cgcggatgca aggctggctt gcgccgacca aggcaaaccc gatatgggcg ttggtgtggc
     5881 tgctgatttg cctgccctgc ctgctgattg cgtccaaatg tttgggttgg aaaggctggc
     5941 ggctgttggc aaacgttctt gcctgcctcg ccgtgcgcac cctgttgagt gtgcctgcca
     6001 cgctgctgat tgcgttcacg ctgcaagacc tgctcaaata ggcgcggaca atggaaagtt
     6061 tcgagcttga ggatttgacg ctgtggctgg tacgcgatgc ggatgaagcg gaaatgtgga
     6121 tagaccgctg ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa
     6181 gcatagggga atggcaggca gggcttcaga cggcatttga acgcatacgc ggcaaatacg
     6241 tcgccgttgt cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag
     6301 acatcctgac acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt
     6361 ttcccgacga tgcggaacac gctttccaac gcgtccgctg tccctgccgt gccgcattgg
     6421 ttgtacccga acacggcggc gtgccgcacg gttgggcgca aagacaggcg gatttgtgga
     6481 acgcccgcct gttggtttcc ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc
     6541 agtggggtat gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat
     6601 ttcggttaac tggggttgca aggaaaatgc cgtctgaaac aggcttcaga cggcatttcc
     6661 attcaaacag ggcttacagg ctgcccaaca cgatacgcag cacgcggcgc agcggctcgg
     6721 ccgcgcccca caagagctgg tcgccgacag tgaacgcgct gatgtattcg ccacccatct
     6781 ccagtttgcg gatgcgtccg acagggacgg acagcgtgcc ggtaactttg gcaggagtca
     6841 gctcgtggat gctggcttct ttttcattgg ggatgacttt cacccagtca ttcgcgcctg
     6901 ccaaaatcgt ttcgatttcg gaaacaggca ggtctttttt caacttcaga gtgatggctt
     6961 ggctgtggca gcgcatcgcg ccgacgcgga cgcacaggcc gtcaatcacg gttggattgt
     7021 cgctgcggcc gaggattttg ttggtttcca cgccgccttt ccattcttct ttggactggc
     7081 cgttgcccaa atccacgtca atccacggaa tcaggctgcc ggcgagcggt acgccgaagt
     7141 tggttttcgg atagtcttcg ctgcgcagga aatcggatac tttgcggtcg atgtcgagaa
     7201 tcgagccggc aggatcggca agctcgtccg ccacttgggc gtgaaccgcg cccataccgc
     7261 tgatgagttc gcgcatattt ttcgcgcccg cgccggaagc ggcctgatag gtcatactgg
     7321 tcgcccattc gaccaaatcg ttttggaaca ggccgcccaa agccatcaac atcagggaga
     7381 cggtgcagtt gccgccgatg tagtttttca cgccgttttt caaaccgttg tcgatgacgt
     7441 tgcggttgac ggggtcgagg acgataatcg cgtcgtcttt catacgcagc gaggatgccg
     7501 cgtcaatcca gtaaccgttc cagccgctgt cgcgcagggc ttggaagacg gatttggtgt
     7561 aatcgccgcc ttggcaggta acgatgatgt ccatttttgc cagttcggca acgttgttcg
     7621 cgtccaataa tgttttagcc gcctgaccga aatcaggggc tgcgccgccg acgttggaag
     7681 tggtaaagaa aaacgcttcg ggaatgtggg cgaagtcgtt ttcttcttgc atacgctgca
     7741 tcaaaaccga accgaccatg ccgcgccagc cgacgaaacc tactttcatt gtgttgctcc
     7801 ttgaggaaaa aatcgttaac aaaattatgt atggcgtaaa ggaagcgttt taacgccgta
     7861 ttgggaaaat gtaaagttct ttatcgggat ttctttaagt attggcgttt tcggtgtcgt
     7921 ggcggtagaa tcggggtcag ttaagaacaa ttatcaggaa cggatatgga agaaaaaaac
     7981 gactacaccg acgcggcatc cgacaaccgc aacgggcagg aaattgaagt cggaattgcc
     8041 ggcgcaggcg accggatact tgccgcgctg ctgaaccaat tgtttacctt tttggttttg
     8101 ttggtgccgt ttgccggact gattgctttc gccatcaaga atgaaggcag gattggcggc
     8161 agtgaggaaa tattcgggct gcttttgggc atgacttcct tttgggtagg actggcgggc
     8221 attttggttt ataccgtcgt tcaaatctac tatatgagtc gggacgggca gtcattgggt
     8281 aagaagatca tgagaatcag ggtgttgaaa accgacggcc gcaatccggg ttttgtcggc
     8341 acggttttgg tacgcgaaat cgcatggtcg gttttggttg ccattattgc cgccgttatc
     8401 ggtcttgcag taggtgacaa cggagaaaac gccatcaacc tgctggcatt ccttgccaac
     8461 tttgtcctgc tctttatggt caaacgcgac cgccgcacgc tttacgacat actggcggat
     8521 acggttgtcg tcaagctgcc caaataaacg gacacggcaa aatgccgtct gaaagccttt
     8581 cagacggcat ttttatcttc agaaccgctc aagcatcgtg cagtgaagcc gcgtgcaggg
     8641 tgttttccat caatgtggca atcgtcatcg gacccacgcc gccgggaacg ggcgtaatca
     8701 tcgccgcccg ttcttttgcc gtttcaaatt ccacgtcgcc gcacaggctg ccgtcgtcca
     8761 aacggttgat gcccacatcg ataacgaccg cgccgggttt gatccattcg cctttgacaa
     8821 agttcggaat gcctacgccg accaccaaaa tatcggctcc ggcaacctcg tctgtcagat
     8881 tttcggttgc gctgtggcag accgttaccg ttgcgcgcgc cagcagcagt tccaaagcct
     8941 gcgggcggcc gacgatattc gacgcgccga ccacgaccgc ttttttcccc ttcggatcaa
     9001 tgccgtaagc ttccaaaagc gtcatcacgc ccttgggcgt acacgggcgc atcagcggca
     9061 ttttgaccgc cagcctgccg acattgtaag gatggaagcc gtccacgtcc ttatccggcg
     9121 aaatacgttc caaaaccgcc tggctgtcga ggtgcttcgg cagcggtagc tgaaccagaa
     9181 taccgtccac ttcggaatcg gcattcaggc ggtcgaccag tgccagcagt tcttcctgcg
     9241 atgttgattc gggcagctcg taagacagtg atttgatgcc gcatttttgg caggcagttt
     9301 tcttgttgcg gacataaacc gcgctggcag ggtcgcctcc gaccaaaacc acggccaggc
     9361 aaggcatgtg cagattgtcc tgttggcgtt gcgccaccgc ttcggcaacc gcctgcaggc
     9421 gtttttgcga aacttcttta ccattgatca gttgtgccga catatccgtc cttttgttaa
     9481 acaggtagaa aatgctgaaa attatcgcat tttccgtttg tttttacagc aggatgccga
     9541 aaataaattt gccctgaaat aaaatacggc tgtttggacg gattaaatat tttttcaaat
     9601 caaaaaacta tatttttcat cttggtgtct tgaaaataaa tattgacgca tctttccctg
     9661 agttgtatag ttcgcttctt caagtcgggg cgtagcgcag cccggttagc gcatctgctt
     9721 tgggagcaga gggtcgtgag ttcgaatccc accgccccga ccaatcaatc agcacgaatc
     9781 ggcatgagcg cccgtagctc aaccggatag agcaccgacc ttctaagtcg ggggttacag
     9841 gttcgattcc tgtcgggcgt gccaagaatt tgattgaaaa ccgtttttat ggtggctgta
     9901 gctcagttgg tagagccccg gattgtgatt ccggttgtcg tgggttcgag ccccatcagc
     9961 caccccagat aaaggcccgt acttttcggt acgggtcttt tcccgtttgc gcgtactgtc
    10021 cgcgccgccg gctttgcctt tcgcatatgc ttctgtatca tgtgggtttt tctgtctttt
    10081 atggtttttg tcggaggctt tgatggttga cgcttggtct gtccctgatt ttgaatcgaa
    10141 aatctgtccg cccgaggcgt tggcggcgcg tttggcgttg ttgccgcgcc cgctggtgtt
    10201 taccaacggc tgtttcgaca tcctccacag ggggcacgtt acttatctgg cgcaggcgcg
    10261 ttcgatgggg ggcgcgttgg tgctggcgtt gaataccgat gcttcggtgc ggcgtttggg
    10321 caagggcggc gaccgcccga ttaatccttt ggagaaccgt gccgccgttg ccgccgcttt
    10381 ggaaagtgtg gatttggtaa cgtggtttga cggggatacg ccggcggcgt tgattgaggc
    10441 ggtcaaacct gagattttgg tcaagggcgg cgattgggct gcggataaga ttgtcggtgc
    10501 ggcagaaacg ttggcgcgcg gcggtcaggt gttttcaatt ccgtttctgc accagacttc
    10561 gacaacgaag actttggcaa aaatccgtgc ggcagagggc ggaaaatgac ggttttgaag
    10621 ctttcgcact ggcgggtgtt ggcggagctt gccgacggtt tgccgcaaca cgtctcgcaa
    10681 ctggcgcgta tggcggatat gaagccgcag cagctcaacg gtttttggca gcagatgccg
    10741 gcgcacatac gcgggctgtt gcgccaacac gacggctatt ggcggctggt gcgcccattg
    10801 gcggttttcg atgccgaagg tttgcgcgag ctgggggaaa ggtcgggttt tcagacggca
    10861 ttgaagcacg agtgcgcgtc cagcaacgac gagatactgg aattggcgcg gattgcgccg
    10921 gacaaggcgc acaaaaccat atgtgtgacc cacctgcaaa gtaagggcag ggggcggcag
    10981 gggcggaagt ggtcgcaccg tttgggcgag tgtctgatgt tcagttttgg ctgggtgttt
    11041 gaccggccgc agtatgagtt gggttcgctg tcgcctgttg cggcagtggc gtgtcggcgc
    11101 gccttgtcgc gtttaggttt ggatgtgcag attaagtggc ccaatgattt ggttgtcgga
    11161 cgcgacaaat tgggcggcat tctgattgaa acggtcagga cgggcggcaa aacggttgcc
    11221 gtggtcggta tcggcatcaa ttttgtcctg cccaaggaag tagaaaatgc cgcttccgtg
    11281 caatcgctgt ttcagacggc atcgcggcgg ggcaatgccg atgccgccgt gctgctggaa
    11341 acgctgttgg tggaactgga cgcggtgttg ttgcaatatg cgcgggacgg atttgcgcct
    11401 tttgtggcgg aatatcaggc tgccaaccgc gaccacggca aggcggtatt gctgttgcgc
    11461 gacggcgaaa ccgtgttcga aggcacggtt aaaggcgtgg acggacaagg cgttttgcac
    11521 ttggaaacgg cagagggcaa acagacggtc gtcagcggcg aaatcagcct gcggtccgac
    11581 gacaggccgg tttccgtgcc gaagcggcgg gattcggaac gttttctgct gttggacggc
    11641 ggcaacagcc ggctcaagtg ggcgtgggtg gaaaacggca cgttcgcaac cgtcggtagc
    11701 gcgccgtacc gcgatttgtc gcctttgggc gcggagtggg cggaaaaggc ggatggaaat
    11761 gtccgcatcg tcggttgcgc cgtgtgcgga gaattcaaaa aggcacaagt gcaggaacag
    11821 ctcgcccgaa aaatcgagtg gctgccgtct tccgcacagg ctttgggcat acgcaaccac
    11881 taccgccacc ccgaagaaca cggttccgac cgctggttca acgccttggg cagccgccgc
    11941 ttcagccgca acgcctgcgt cgtcgtcagt tgcggcacgg cggtaacggt tgacgcgctc
    12001 accgatgacg gacattatct cgggggaacc atcatgcccg gtttccacct gatgaaagaa
    12061 tcgctcgccg tccgaaccgc caacctcaac cggcacgccg gtaagcgtta tcctttcccg
    12121 accacaacgg gcaatgccgt cgccagcggc atgatggatg cggtttgcgg ctcggttatg
    12181 atgatgcacg ggcgtttgaa agaaaaaacc ggggcgggca agcctgtcga tgtcatcatt
    12241 accggcggcg gcgcggcaaa agttgccgaa gccctgccgc ctgcattttt ggcggaaaat
    12301 accgtgcgcg tggcggacaa cctcgtcatt cacgggctgc tgaacctgat tgccgccgaa
    12361 ggcggggaat cggaacatac ttaaaaaata cacccttttt caacacgggg cggcatccgt
    12421 ccgaatccgt ccggattctg ccgcccgaca gactgccgcc caggcgggcg gggtttcaaa
    12481 cattgaggaa attctaatga aatggctctt cacccttttg gcggtactca atattgccgt
    12541 attcggcggc acggtgggct ataaattggc cgttaaagcg acgggcggcg tgccggaaaa
    12601 ccgggccgtc gaaaacacgc cccccgcaac gcccgccgcc gacaatgcgg cggcatccgt
    12661 ggaggatacg gcggcactgc tcaaaccggg cgacatcctg accgaagagc aggccgagca
    12721 gttgcgcttg aaaaaggaag cggaacagaa aaaactgagg gagaaaaaac agcgtgaaga
    12781 aaaagcccgc cgcgaaaaac tcgccgccga aaaggcgcag gcggaacgcg aaaacggcgc
    12841 ggcggatgcc ttatgcgccg cgcaggcaag cctcacgatg gacgaagacg actaccaccg
    12901 catcaaaggg cttttgggca aatggtcgca cgttgccagc aggagcgtcg aaaaacgcac
    12961 cgccaaagcc aaacctgccg acaaaaccta ccgcgtcgtc ctgcccgttt ccgccgatgc
    13021 cgaaaatcag gcggcggagc tgtctgccaa aggtttcaac cccataccgt ttgacggcgc
    13081 attgagtttg ggtgtcggca acagccggga aaacgcccaa gccctgcaaa accggcttgc
    13141 cggcgccgga ttcggcgggg cgcatattgt cgaacacttt gccgaagccg acaggcagga
    13201 cgattctttg agcgtgtcgc gtatgacggt tttgtttacc ggcgtgaatg ccgccgatgc
    13261 ggacgaaatt cgtaaaatca cgtccctata cggcaaactg aacctcaagt cttgcaaata
    13321 ggcgcgaaag ccggacgata aacgccaaac ccaccgcgaa ggtgggtttg gcgtttcggt
    13381 tttgagtatc tgatatataa aaagggaatg tatgtctcgt atatgccgac tccaagtgtg
    13441 aaagtgaatt gtgagggaat acagtgttta atggttcgga atggacgact tccgagtggt
    13501 tttgattata cgctaataat aataattatc aataaaaatt taaaaataaa tgcggatttt
    13561 ttgtaatacg atgaaaaata aacaaattaa cctgtccgcc gatgtttgtg attcgggtcg
    13621 gcaaactttc attgtgtcgg acaaatcggg gtcggcagtc atagctgagg gcttcgtgcc
    13681 tgacggaaat aagcctgccg tgtttgaaca tccgtccgat ttggcgcgga cagggttgtc
    13741 attcgggggt gtttgagcgt ggattcgggg atgcgaaagc ggtaaaaccg ttgcggatga
    13801 aggcttacgg acggctttag gtatttttaa gtgtattcaa acaacaacaa aacgcttctt
    13861 ctattgccgt atttagggct tgcgcggcag cctgcacacc gtggaggccg agtgtttcgt
    13921 tggttgcggc atcgggacgg gcggcaaggt ttttcggccg gtttttccat tttttccgac
    13981 aaagccgtga tgccggggaa tggcggtttg tccgtttcag gccgccgccg ttttggaccg
    14041 cgatgccgtc tgaagccttc agacggcatt tttgccgcct ttttcaatat tccgccgaat
    14101 gccaaaacgg ttgtccgact gtcggcgtgc tggcttgcgc tttgtcgcgt gcttcgaccg
    14161 gtccggcttc aaatgccagc cgtccggatt cgacggcgag tgcgaaggcg cgtgccatat
    14221 tgaccggatc gccgctgcgg gaaacggcag tattcaaaag cacgccgtca aagccccatt
    14281 ccatcacttg tgccgcctgt gagggcaaac ccaagcccgc gtcgataatc agcggcgtgt
    14341 cgggcaggcg ttcgcgcagg acgttcaacg cgtaggcgtg aaccgcgccc aaacccgtgc
    14401 cgatcggggc cgcccacggc atcaacgcct gacagcccgc gtcgagcagg cggcggcagg
    14461 caatcaggtc ttcggtgcaa taaggcagca ctttgaagcc gtctttaatc aggatttccg
    14521 ccgcttcgac aagctggaac acatccggct gcaaggtgtc gtcatctccg atgagttcca
    14581 attttatcca atcggtttca aacacttcgc gcgccatttg cgccgtcgtt accgcttcct
    14641 gcacgctttg gcagcctgcc gtgttcggca ggacgggaac gccggtttct tgaagcagcg
    14701 accaaaaccc ctgaccgtgc gcctcgtcgc cgcttcccgc gcggcgcaat gagacggtaa
    14761 tcatcgcggg ttgggcgata cggacggatt gtttgaggat ttcaggggtc gggtaggcag
    14821 ccgtgccgag cagcagccgt gaagggaaag tttctccgta tagggtgagc ataatgggtt
    14881 cctttgtata gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaaa
    14941 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt gtactgtctg
    15001 cggcttcgtc gccttgtcct gatttaaagt taatccacca taacgttgtt ttggggacag
    15061 gggtcgtctg aaaaggcaaa accgcctagc cgccgaccac cggccgcacg atatcgattt
    15121 tgtcgttttc gttcaaaacg gtttccgcat acgcgccttt ggcgacaaat ccggtattca
    15181 ccgctacggc aaaaggcttt tgcggcgcgg tttgggcgat gaggtcggca acggtcgtgc
    15241 cgtgaagttc ggcgggttcg ccgtttaaga tgatgttcat ggtttctcga tgtttctg
//
LOCUS       DAXIHS010000047        15071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXIHS010000047 DAXIHS010000000
VERSION     DAXIHS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.047"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            82..891
                     /gene="aroE"
                     /locus_tag="ACFOF2_001270"
     CDS             82..891
                     /gene="aroE"
                     /locus_tag="ACFOF2_001270"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC7107586.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            894..1595
                     /gene="mtgA"
                     /locus_tag="ACFOF2_001271"
     CDS             894..1595
                     /gene="mtgA"
                     /locus_tag="ACFOF2_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC7107587.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(1920..1995)
                     /locus_tag="ACFOF2_001272"
     tRNA            complement(1920..1995)
                     /locus_tag="ACFOF2_001272"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:Phe,seq:gaa)
     gene            complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="ACFOF2_001273"
     CDS             complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="ACFOF2_001273"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC7107588.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPALSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="ACFOF2_001274"
     CDS             complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="ACFOF2_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC7107589.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQGDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISADRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="ACFOF2_001275"
     CDS             complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="ACFOF2_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC7107590.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(4083..4619)
                     /locus_tag="ACFOF2_001276"
     CDS             complement(4083..4619)
                     /locus_tag="ACFOF2_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC7107591.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(4837..5811)
                     /locus_tag="ACFOF2_001277"
     CDS             complement(4837..5811)
                     /locus_tag="ACFOF2_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC7107592.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            5908..6963
                     /gene="tal"
                     /locus_tag="ACFOF2_001278"
     CDS             5908..6963
                     /gene="tal"
                     /locus_tag="ACFOF2_001278"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC7107593.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            6978..7112
                     /locus_tag="ACFOF2_001279"
     CDS             6978..7112
                     /locus_tag="ACFOF2_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107594.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGFALKILMGKKLMHDITRQHKRK
                     "
     gene            7117..7407
                     /locus_tag="ACFOF2_001280"
     CDS             7117..7407
                     /locus_tag="ACFOF2_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107595.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            7424..8311
                     /gene="gluQRS"
                     /locus_tag="ACFOF2_001281"
     CDS             7424..8311
                     /gene="gluQRS"
                     /locus_tag="ACFOF2_001281"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC7107596.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGRWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWYGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(8381..9391)
                     /gene="dusA"
                     /locus_tag="ACFOF2_001282"
     CDS             complement(8381..9391)
                     /gene="dusA"
                     /locus_tag="ACFOF2_001282"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC7107597.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(9422..9913)
                     /gene="dtd"
                     /locus_tag="ACFOF2_001283"
     CDS             complement(9422..9913)
                     /gene="dtd"
                     /locus_tag="ACFOF2_001283"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC7107598.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(9987..10745)
                     /locus_tag="ACFOF2_001284"
     CDS             complement(9987..10745)
                     /locus_tag="ACFOF2_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC7107599.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(10816..11682)
                     /locus_tag="ACFOF2_001285"
     CDS             complement(10816..11682)
                     /locus_tag="ACFOF2_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC7107600.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(11731..12009)
                     /locus_tag="ACFOF2_001286"
     CDS             complement(11731..12009)
                     /locus_tag="ACFOF2_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC7107601.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(12009..12299)
                     /locus_tag="ACFOF2_001287"
     CDS             complement(12009..12299)
                     /locus_tag="ACFOF2_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC7107602.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(12302..12832)
                     /locus_tag="ACFOF2_001288"
     CDS             complement(12302..12832)
                     /locus_tag="ACFOF2_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC7107603.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            13140..>15071
                     /gene="tspA"
                     /locus_tag="ACFOF2_001289"
     CDS             13140..>15071
                     /gene="tspA"
                     /locus_tag="ACFOF2_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC7107604.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPS"
BASE COUNT         3528 a         4342 c         3821 g         3380 t
ORIGIN      
        1 ctgaaaacag tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc
       61 ccgccttgat ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc
      121 gtcgcccaca gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac
      181 attgaatacg aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt
      241 tttgaaacag gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat
      301 ctggcggacg agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt
      361 ctgaaaaacg gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc
      421 acgcaggtca aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc
      481 gcggtgcgcg gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc
      541 aaccgcaccc acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg
      601 atggcggatg tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc
      661 ggtcagcttc ctgccgtcag ccgtgaaatt tttcgcaact gccgccttgc ctacgatatg
      721 gtttacggcg acgcggcgca ggcgtttttg aactttgccc aaagcaacgg cgcggccgaa
      781 gtttcagacg gcctgggcat gctggtcggt caggctgccg cctcctacca tatttggcgc
      841 gggtttacgc ctgatatccg ccccattatc gaatacatga aagccctgta accatgttcc
      901 gcatcatcaa atggctgatt gccctgcccg tcggcatctt tatctttttc aatgcctatg
      961 tgtacggcaa catcattacc taccgcgccg tcgcgcccca tcagactgcc tttatgtcga
     1021 tgcggatgaa gcagtttgaa caggaaggtc gcgatgtcgc actggattac cgctggatgc
     1081 cctacaaccg catctcggtc aacctgaaaa aagccctgat tgcttccgaa gatgcccgtt
     1141 tcgccgggca cggcggcttc gattggggcg gcattcaaaa cgccatcagg cgcaaccgga
     1201 acagcggcaa agtgaaggcg ggcggctcga ccatcagcca gcagcttgcc aaaaacctct
     1261 tcctcaacga aagccgcagc tatatccgca aaggcgaaga agcggcgatt accgcgatga
     1321 tggaagccgt taccgacaaa gacaggattt ttgaactgta tttaaactca atcgaatggc
     1381 actacggcgt tttcggcgcg gaagccgcgt cccggtattt ttatcaaata cccgccgcca
     1441 agctgaccaa acagcaggcg gcaaaactga cggcgtgcgt ccccgccccg ctctactacg
     1501 ccgaccatcc gaaaagcaaa cggctccgca acaaaaccaa tatcgtgctc agacgcatgg
     1561 gttcggcaga gttgcctgaa agcgacacgg actgattgtt tcagatatgg aaatgccgtc
     1621 tgaactgcgg ttcggacggc atatgttttc tggtttttat agtggattaa caaaaatcag
     1681 gacaaggcga cgaagccgca gacagtacaa atagcacgga atcgattcac ttggtgcttc
     1741 agcaccttag agaatcgttc tctttgagct aaggcgagcc aacgctgtac cggtttaaat
     1801 ttaatccact atacctgccc gacagccgca aagaccgtat catacatagc aggcattgcg
     1861 gtgcgggatt actgccgcaa acggcaggca aaaaaaataa ccgcttcaag gcggtttttt
     1921 ggtggccaga ggcggaatcg aaccgccgac acacggattt tcaatccgtt gctctaccaa
     1981 ctgagctatc tggcctttgt ctgtctcgtt aagagatgcg tattaaacca aattacggga
     2041 cgacatgcaa gcatatcggg caatattctt tgaaaacagg tttaagtcaa tgttttaaat
     2101 ttgaattaat ttatgtaaaa acaacataaa tccgtatttt tgccgcctgc ccgacgatat
     2161 taggtcgccc gtctgaaaaa tattttcaat atttgaagtt cttacccaga taaacagaac
     2221 gaacctgttc gtttccgacc aaatcatcag gttttcccga tgccagcacc gcaccgtctg
     2281 aaatgatgta ggcgcggtcg cagatgctga gggtttcgcg tacgttgtgg tcggtaatca
     2341 gtacgccgat accgcgcgac ttgagaaatc cgatgatttt ttggatgtcg atgacggcga
     2401 tgggatcaac accggcaaaa ggctcatcca gcaaaataaa atgcggtttc atggcgagta
     2461 cgcgggcgat ttcgacgcgc cgccgttcgc cgccggataa tgccggcgcg gggctgcggc
     2521 gtaggtgtcc gatattgagg tcggcgagca gtttttcgat ttctctgtcg atttgatttt
     2581 tatctttggt tctgatttcc aagatggcgc ggatgttttg ttcgacggtc attttgcgga
     2641 atatcgaggc ttcctgcggc aggtagccga cacctaggcg ggcgcgttcg tgtatgggca
     2701 ggtgacgcaa ttcctgcccg tccagcatca cactgcccgc atcggcggca atgagtccga
     2761 ctatcatgta gaagctggtg gttttacccg cgccgttggg cccgagcagg ccgacgactt
     2821 ctccgctttc gatttcgagg gagaagcttt taacgacttg gcgttttttg aaactttttt
     2881 gcaggttttg aacaacaagg cggctgacgt ttgcgctcat aaatatctct tcaatatctt
     2941 cggtcggcaa atgccgtccg aaccaggttt atgtttcaga cggcattttg ggattattcg
     3001 gatttttgcg tactcgaagg ctggataacg acgctgaccc tgccggattt ggaagcggat
     3061 tttgcgccgg attttgtgct gccgctgatg gtatagactt cggttttggt gttgtatgta
     3121 atcaccgcac cttcggcgac atcgccgccg cgctgtactt tggcattacc ggttaagact
     3181 acggtgctgc ctgcagatga ataagcaacg ttgtttgcct gtccgcgcac cgtgcctttg
     3241 ccgccgtcca atgtctggct gaagcggact ggcgaacctt ccgccctcac ggattcgccg
     3301 cctttgccgc cgcgtgtaac gttgacgcgg tcggcggaaa tattgagcgt accctgtctg
     3361 atgacaacgt ttccgctaaa tgtagtgctt tggttggctt gattgagaga accttggtcg
     3421 gcctcaatct gaataggctg cctgctgtcg ccttgaaggg caaaagcggg agatgccgaa
     3481 aaaaatgcta ttaaaacaaa tagcttacat atcttttgta tcataaatcg tggctttcac
     3541 tttagatgag aagttcaaca tgcctgtttt gtggtcgtaa gtcatgccgc ccgcctgccc
     3601 gtgcgatgca ccatattgga aactgacagg cgtatcggtt tgggcatatt gagattcggt
     3661 atcgacgtgc agcttttcgg cttcaacttt acccgcctgc cgtttgccgt cggcggtttt
     3721 ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt tcggtatggt aaacggcttc
     3781 atcgctgccg acttcgtaca acaacctgcc ttcttggaag aacacgagat gcggtgagtc
     3841 gaaatgaatg tcgctgcttt cgggaaactg tttcgcgccc ttcgaactca aatgttcttt
     3901 caagtatccc tgttcgtcaa accgcctgcc atccaatccg tccattgtgt attgcggttc
     3961 gtcgggattg agcctgactt cttcaatctc gacttcgctg atgcgtccca accatgccga
     4021 caggctgccc aaggcaaccg ccaatatcaa tgggaacgca attccgtacc gccatcttac
     4081 tttcatttga tgtactcgtt caaagccgcg cccaaagtcc cttgcgcctg cataatcagg
     4141 tcgcacactt cgcgcaccgc gcccgcaccg ccggggcgtt tggcaatata ggcggcgtgt
     4201 tgcagcgtaa accaatgcgc gtcggggacg gcaaccggca atccgcagcg taccataacg
     4261 ggcaaatcga ccacgtcgtc gccgacaaag gcacactcgg cttcttccac gccgacctga
     4321 gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc ctttgaagta gtaatttatg
     4381 cccaactgtt tgacgcggat gccgacggag ggcgcgtccc gacctgtgat aatcgcagtc
     4441 tgcacgccgc ttgcctgaag cattttcaga ccgtgtccgt ccagtgtgtg aaacgatttg
     4501 atttcttcgc cgttatcgcg gataaagatg cgcccgtcgg tcaaaacgcc gtccacatcc
     4561 aggatcaaca gtttgatttt ggcggcgcgc gcctgtaatt cgggagaaat tgcctgcatg
     4621 ggatttcctt gttttcagac ggcatatctt accacaaatg ccgtctgaaa cggctgcaac
     4681 gccttatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga
     4741 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta
     4801 aggcgaggca acgccgtact ggtttttgtt aatccactat acaatccgcg ccgccaaaag
     4861 gtcgtgcata ttcagcgcgc cgatcagcac gccatctgca tcggtaacca gaagcccgtt
     4921 cacatggttt gcctgcatga ctttcagggc ttcggtggcg agacgttcgg cggagatggt
     4981 tttaggatgc gtatgcatga cttcgtctat cgaaagaccg gtaaaattgt cgcattcttg
     5041 aaacaggcgg cgcaaatcgc cgtcggtgaa tacgcctttc agacggcctt gcccgtccgt
     5101 taccgccaac atgcccagcc ctttttcact catgctgacg atggcttctt tcaagggcgt
     5161 gccgagtcgg acggcaggca ggccgccgcc tttgtgcata atgtcggcaa cgcgcaaaag
     5221 taggcgtttg ccgaggctgc cggcagggtg gctcaaggcg aagtcgtcgg gcgtgaacgc
     5281 gcgggcgcgc agcaggacaa ccgccaacgc atcgcccaaa gccataacgg cggtggtgct
     5341 ggtggtcggg gcaagcccca gcgggcaggc ttctttggaa accgacgccg tgatgtggat
     5401 gtcggcatgg cgcgccatgg ttgaatcggg gcgggcggtg atgcagacaa gcgtgatatc
     5461 tttgcgtttg agcgcgggga tgatggcggc gatttcgtcg ctttcaccgg aattggaaat
     5521 cgcgacgacc acgtcgttgt ccacaatcat gcccaaatcg ccgtgtgccg cttccgcagg
     5581 gtggacgaaa aacgcgggcg tgccggtcga ggccatggtt gccgccattt tgcgcccgat
     5641 atgtcccgac ttgcccatgc ccgtgataac gaccctgcct ttgcagtgca acaacgcgtc
     5701 cgccgcaagg gcgaagtttt cgtccaaatc cgccgcaatt tcgcgcaagc cttccgcttc
     5761 ggtgtgcaac acttcgcgtg cccaatcaag atatttttcg tttcctgcca tcgccatccg
     5821 cctttgctgt aataaaacta cccaaaattt ccgaatcggg gtagcataag cctttgaaac
     5881 atacacatcc atccaaagga atcagaaatg actattttat cggacgttaa agcattagga
     5941 caacaaatct ggctggacaa cctttcccgc tcgctcgtgc aaagcggcga attggcgcaa
     6001 atgctgaaac aaggcgtgtg cggcgtaact tccaatcccg ccatctttca aaaagccttc
     6061 gccggcgatg cgctttacgc cgacgaggtt gccgccctca agcagcaaga cctgaccccc
     6121 aaacaacgct acgaaacgat ggcggttgcc gatgtgcgtg ccgcctgcgg cgtctgtctt
     6181 gccgaacacg aatccaccgg cggcaaaacc ggcttcgtca gcctcgaagt ttcgcccgaa
     6241 ttgtccaaag acgcgcaagg cacggttgaa gaagcgcgcc gcctctacgc cgccatcggg
     6301 tgcaaaaacg cgatgattaa agtgcctgcc accgacgcag gcatcgatgc gctcgaaacc
     6361 ctcgtttcag acggcatcag cgtaaacctc acgctgctgt tctcacgcgc ccaaaccctc
     6421 aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct tggcagccgg acaaagcgtt
     6481 gcccatatcc acgtcgtcgc cagcttcttc atctcgcgtg tggacagtgc gctggacaca
     6541 acactgcccg accggctcaa aggcaaaatc gccatcgccc ttgccaaagc cgcctatcaa
     6601 gattgggaac aatatttcac cgcccccgaa tttgccgcac tggaagccca aggcgcaaac
     6661 cgcgtgcagc ttttatgggc atctaccggc gtgaaaaacc cagcctatcc cgacacgctc
     6721 tacgttgaca gcctgatcgg cgcgcacacc gtcaacaccg ttcccgatgc cacgctcaaa
     6781 gcctttatcg accacggcac agccaaagcc acgctgaccg aaggcgtaga agaagcacaa
     6841 gcgcagctcg ccgaaaccgc cgcgctcggc atcgatgtcg aaaccttggc aacgcgtttg
     6901 caggaagacg gtttgaaaca gtttgaagaa gcgtttgaaa aactgctcgc accgttggtt
     6961 taaacttttc atccccgatg ccgtctgaaa ggctttcaga cggcatcgcc cttttgaccg
     7021 gacacagtgc ggcatatcaa ggattcgccc taaaaatatt aatgggcaag aagttaatgc
     7081 acgacattac ccgccaacac aaaaggaaat agaaccatag caaaagcact cgaaatcatt
     7141 tcacccaatg aaatttattc agatctgatt tttaaggatc cggtacctcc ccatacaata
     7201 tatacagaat tgatgaaact ggttagtaga aatgcaagta atgagagaat cgcagataga
     7261 gccttcgatt ttttctcgtc cggccttatg gatggttcag ctaccgaaga gcaatataat
     7321 gccctatatg atttaacctt acttgaagaa cccgggatgg aactgaataa agatgagata
     7381 atggctttga ttaatagtct taaataaagg ggccgtaccg gatatgtaca caggacgttt
     7441 cgcccccagc ccgaccgggc tgctccacat cggctcgctg ctgaccgccg tcgcttccta
     7501 tgccgatgca cgcgcacacg gcggaaggtg gctggttcgc atggaagacc tcgatccgcc
     7561 gcgcgaaatg ccgggggcgg caagccatat cctgcacacg cttgaggcat tcggattcga
     7621 gtggtacgga gaagtcgcct atcagagccg ccgttacgcc ctgtatgaag aaaccctatg
     7681 ccgtctgaaa accgccggac tggtctatcc ctgccattgc agccgcaaag actggcaggc
     7741 tgcggcagtc cacggcgcag acggattcgt ctataacgga cgttgccgca atccgcagca
     7801 acgccctgca ctgcaaggca aacagcctgc gtggcgcatc cgcgtccccg accgcgacat
     7861 cggcttttca gacggcatcg tcggcggtta cgcccaaaac ctcgcccgcg acatcggcga
     7921 tttcgtcctg cttcgtgcag acggttactg ggcataccag ctcgccgtcg ttgccgacga
     7981 tgccgaacag ggcgttaccc acatcgtccg cggacaagac ctgcttgttt ctacgccgcg
     8041 ccaaatctat ttgcagcaat gtttgggcgt tccgacaccg caatatgccc acctgccgct
     8101 gctgaccaac gcacaagggc agaaatggtc gaaacagacg ctcgcacccg cattggattt
     8161 aaaccgccgc gaacaactcc tccgccaagt gttccgttac ctcaacctgc ccgaagcacc
     8221 ggaagccgac cgccctgccg aactgctcga ctgggcggtg gcacactggg atatggacaa
     8281 agtgccgaaa cacgccatta ccacccccta aacaccgccc gacaaacgca caaatgccgt
     8341 ctgaagcctt cagacggcat ttcacacata cagccccgcc ctattcgcgc gtatttgccc
     8401 gttcgacctc tttccacgct tcgagaatca ggctgccgtc gttgtctttc aagagcgttg
     8461 cgtcggaaag catacgccgc caagtccgcg cgcctttcaa gccgtgcatc agcccgaggc
     8521 tgtggcggac gatatggcgc aggattgtgc cgcgtccggc ttggatttgg gcttggctgt
     8581 atgtgtagag gcgttgcacc aaatcggcgt atccgatcgg gttcgcagtg tcgccgtaaa
     8641 acaatctgtc ccattcgcgc atcaccatcg ggttgtggta cgcctcgcgc ccgaccatca
     8701 cgccgtcaac gtgttgcagg tgtccggcga ttgcttcgtt ggtggtgatg ccgccgttga
     8761 tgatgatttc cagcccggga aactcctgct tgaggcggta aacgtaatcg tatttcaacg
     8821 ggggaacgtc gcggttttct ttgggggaaa gaccgtccag ccaagcgttg cgggcgtgga
     8881 cgatgaaggt tttgcaggcg gttttgtcgc gcaacgtgcc gacgaaatcg gcaacggttt
     8941 ggtattcggt ctgcctgtcc acaccaatgc ggtgtttgac ggtaacggga ataccgaccg
     9001 cgtcctgcat ggcgttgagg cagtcggcaa ccagcccgac ttcgttcatc agacacgcgc
     9061 cgaacgaacc tttctgcacg cgcggactgg ggcagccgca gttgaggttg acctcgttgt
     9121 agccgtatgc ttcggcggct ttggcggctt tcgccaaatc ggacggatcg ctgccgccca
     9181 gttgcagggc gacgggctgc tcgccttcgt tgaacatcaa aaagcggtct ttgtcgccgt
     9241 aaacaatcgc accggcattg accatttcgc tgtaaagcca agcatttcgg gtaatctggc
     9301 gcgccatata acggtagtgc ctgtccgtcc agtcgagcat cggggcgacg gaaaggcggc
     9361 gcgggggaag ggtgtgtgta ttgtcgttca ttgtcgatat ttcatcattg cagattcaat
     9421 ttcaatccgg aacaaccttc atttttgggg aaatccgcgt catgaaagag tccagcagta
     9481 tggttaccgg cccgtcgttg cagagcgaca cctgcatatg cgtgcggaaa cgccctgttt
     9541 cgacatgaat cccgtgtccg cgcaacagtt ccgccgttcg caggtaaagc tgctgcgcct
     9601 gttctgcagg tgcggcttgg gaaaacgaag gccgccgccc gcttgccgca tcggcataaa
     9661 gtgtaaactg cgacaccagc agcaccgcgc cgccgacatc tttcaaagac aggttcaact
     9721 tgcccgcttc gtcttcaaac acgcgcaaat gggcgatttt gtcggcgata tagtgcgcat
     9781 ctttttctgt gtcgctatgc gttacgccga gcagcacgac gaatccgccg tcgattttgc
     9841 cacaggtttc cgtgccgttt tcggacacga catccacctt tgcacctacc gttttctgta
     9901 tgaccgcacg cataagccgt cctccttacc tgaacgtgca atgccgtctg aaaaggcttc
     9961 agacggcatc ggtattggaa atgccattac ggtttgacac cgttttcttc caaaatggca
    10021 tcgattttca agcgggcttt ttcctgtctc aaaccttgtt ccaactggtt tctgaccaac
    10081 tcgaaaggct gcgcgtcggg gtttttcccg acctcgctga gtttgaacag ataatagcgt
    10141 tcgcccaatt tgaccggatc gcgggtaacg tcgccccgat tcatcgctgc aaactgcgaa
    10201 gccagcggct cgggaagctg ctgcgccata atgaaaccgt caaaagcctg ctcgtcgttc
    10261 ggataacgct tcatcagccc ttcaaaagac agccctttga gcaggagctg ctgcgcctga
    10321 cgcgcctcct cttcggttgc gaagctgacc tgctgcaatt tgatcatgcg gatttgccgc
    10381 tcataaaatt gacgcagtgc gctttcggaa accgtttccg aacgttccag aaaacggacg
    10441 tactcctcgg cataaaaaga cgcttcggcg attttaaagc ggttttggac atccttatcc
    10501 ttatccaaac cttccttcaa tgccctgttt ttcaaaactt ccaaagtttg cagccgacgg
    10561 acggcatcgt ttcggattgc ctgcccgtcc ggtttttggg actgctccgc atgccggtct
    10621 gcctgctgca tgatctgcgc caccagcgta tccaccaaag ccgggtcgat ttcgggtgct
    10681 ttggctgccg caaaacctgc caacattgca gcaataactg cggcagcggt ttttttctgt
    10741 ttcatggtat tccctgtcct gcgcggcggc aaaacgcctg tctgaacgtc ttgccgccgt
    10801 atcccgtttt cagaattatt ttgcaggttt gatgtttgcc ttgcccaaca gtgcaccgac
    10861 ggcacggtca atccgttccg cctgaaggtt gccggcaatc tgtcctttca tttcatcaaa
    10921 agaaggcact ttcacctcgc ggctgtcgtt gacataataa acgccgtaga aatcgccgtt
    10981 tttcagcggc gttgccgtaa attcgccttt tttcaagtcc ttaattgcct gataaagcgg
    11041 cggaacaccc tgttccaaat ctttcagcgg cacatatccg accggtgcgc cggtctgttt
    11101 ggtacggtcg ttgagggaat attgtttcaa gacggcatcg aaaccttttt tcgccttcaa
    11161 gtcggcaacc gctttttttg cattttcttc cttgtcggtc aggatttcgc ccaactggac
    11221 ttcctgcgta cctttgtaaa aaccgctgat attgtcatat gcggctttta cttcctgctc
    11281 ggaaaccggt tgggttttgg cgatatgcag cgcgtatgcc tcgccgttca agccatattt
    11341 taccgcctgc caaacggttt tgaaggacgg tttcttgtcg tcgcccgact ttttcgcttc
    11401 ggcacgcaat ttggcaagcg catttttaaa ctctgccgac cggtcgagtt tcaggcgttt
    11461 cacttcctgt gcgaccacgg tgttgaccac ttcgttttcc agcagggatt ggcgcagttg
    11521 cggcgtgtct tcggcacggc tgttttccgc acggaatgcg gcaacctgcg catcgatgac
    11581 ggaactgtcg attttctgac cgttgacggt tgccagcgtt tgggcaaaca ggctgccgga
    11641 acaggcaagc agtgcaacgg aagtcaggat ttttgctttc ataatcttct ctctttaaat
    11701 tttaaattga aatgttcggg cagacttcat tcagtccgcc gtatggaaat actcgtcagg
    11761 ggtagccgcc cggatgccga gcgcgtgaat catgccgcct gaaaacaaat ctttgagcag
    11821 cgatttgacc gttttctggc ggttcaggcg gcttacgcct tcaaaacggc cgctaacgac
    11881 caaaacggca taatgtccgc cgccggtatt gcccgcgtgt cctttgtgca gatggctctc
    11941 atcgccgatt tccaacacca gcggatcgag cgtctgcagg cgttcgcgga tcaaatcgac
    12001 ggccggcatt atttgaacac cgctttaaac ggcttgatca gcacttcgct gtacacgcct
    12061 gcatgaacat agggatcgtc ttccgcccac gcctctgccg catccaaaga ctcgaactgc
    12121 gccacaatca aactgcccga aacgcggttg gagtcctccg gcaaaggatt cgggcctgcc
    12181 gtcaacagcc ggccttccga cttcagcgtc tccagccgtt tgaggtgttc gggacgtgcc
    12241 gccatacgcg cttcatacac attctccccg tctgttgcca gcaacataaa atattccaca
    12301 gtcaatcctc ctttttcaga caggtactca gataaatacc ctgaataata aagaaaacaa
    12361 gcatcagtgc agtcgagccg aacattttat agttgaccca ttgcgactcg aaccgggtaa
    12421 acacaaacca gttggcgata cccataaaaa tcaggaaacc gacccacata tacgtcaatt
    12481 tcgcccatac ggcatccgga agctgaatct ccctgccgat actcgccttc aagccgtttt
    12541 taccggcgag gtggctgccc cacaggaaca gcgcgcccag ccaaaacaaa acgctcggct
    12601 tccacataat gaagcggctg tcgcccaaaa caatggttgc gccaccgaat atcacaatca
    12661 gcaccagtcc gacccactgc atcgtatcca gctttttata tttccaatac agaaaagccg
    12721 cctgaaccac accggcaacc aaggcgaccg ccgtagcggc aatcatattt ttggtaacgg
    12781 tataggtggc gaaaaacagg atgacggaca aaaggtcgct gacgaatttc atatccaaat
    12841 gagaaagcaa atcaaataaa cgctaatcat acaggcagaa ccctgttttt gcacctgcaa
    12901 aaccaacaaa ttttgttttg attgatgcaa atatgcacag atgtttttga aaaaagatgg
    12961 agatatgtca taatttgtaa aaacggcgaa tgattgccgt ttaaaatgtg gcggcggtcg
    13021 gtacattcac atattaaatg cccgccggcc gactgccccg ccgatgcccg atgatgacga
    13081 aatttacaga ctgtacgcgg tcaaaccgta ttcagccgcc aacccacagg ggatacatct
    13141 tgaaaaacaa cagacaaatc aaactgattg ccgcctccgt cgcagttgcc gcatcctttc
    13201 aggcacatgc tggactgggc ggactgaata tccagtccaa ccttgacgaa cccttttccg
    13261 gcagcattac cgtaaccggc gaagaagcca aagccctgct aggcggcggc agcgttaccg
    13321 tttccgaaaa aggtctgacc gccaaagtcc acaagttggg cgacaaagcc gtcattgccg
    13381 tttcttccga acaggcagtc cgcgatcccg tcctggtatt ccgcatcggc gcaggcgcac
    13441 aggtacgcga atacaccgcc atcctcgatc ctgtcggcta ctcgcccaaa accaaatctg
    13501 cactttcaga cggcaagaca caccgcaaaa ccgctccgac agcagagtcc caagaaaatc
    13561 aaaacgccaa agccctccgc aaaaccgata aaaaagacag cgcgaacgca gccgtcaaac
    13621 cggcgtacaa cggcaaaacc cataccgtcc gcaaaggcga aacggtcaaa cagattgccg
    13681 ccgccatccg cccgaaacac ctgacgctcg aacaggttgc cgatgcgctg ctgaaggcaa
    13741 acccaaatgt ttccgcacac ggcagactgc gtgcgggcag cgtgcttcac attccgaatc
    13801 tgaacaggat caaagcggaa caacccaaac cgcaaacggc gaaacccaaa gccgaaaccg
    13861 catccatgcc gtccgaaccg tccaaacagg caacggtaga gaaaccggtt gaaaaacctg
    13921 aagcaaaagt tgccgcgccc gaagcaaaag cggaaaaacc ggccgttcga cccgaacctg
    13981 tacccgctgc aaatactgcc gcatcggaaa ccgctgccga atccgccccc caagaagccg
    14041 ccgcttctgc catcgacacg ccgaccgacg aaaccggtaa cgccgtttcc gaacctgtcg
    14101 aacaggtttc tgccgaagaa gaaaccgaaa gcggactgtt tgacggtctg ttcggcggtt
    14161 cgtacacctt gctgcttgcc ggcggaggcg cggcattaat cgccctgctg ctgcttttgc
    14221 gccttgccca atccaaacgc gcgcgccgta ccgaagaatc cgtccctgag gaagagcctg
    14281 accttgacga cgcggcagac gacggcatag aaatcacctt tgccgaagtc gaaactccgg
    14341 caacgcccga acccgctccg aaaaacgatg taaacgacac acttgcctta gatggggaat
    14401 ctgaagaaga gttatcggca aaacaaacgt tcgatgtcga aaccgatacg ccttccaacc
    14461 gcatcgactt ggatttcgac agcctggcag ccgcgcaaaa cggcatttta tccggcgcac
    14521 ttacgcagga tgaagaaacc caaaaacgcg cggatgccga ttggaacgcc atcgaatcca
    14581 cagacagcgt gtacgagccc gagaccttca acccgtacaa ccctgtcgaa atcgtcatcg
    14641 acacgcccga accggaatct gtcgcccaaa ctgccgaaaa caaaccggaa accgtcgata
    14701 ccgatttctc cgacaacctg ccctcaaaca accatatcgg cacagaagaa acagcttccg
    14761 caaaacctgc ctcaccctcc ggactggcag gcttcctgaa ggcttcctcg cccgaaacca
    14821 tcttggaaaa aacagttgcc gaagtccaaa caccggaaga gttgcacgat ttcctgaaag
    14881 tgtacgaaac cgatgccgtc gcggaaactg cgcctgaaac gcccgatttc aacgccgccg
    14941 cagacgattt gtccgcattg cttcaacctg ccgaagcacc gtccgttgag gaaaatataa
    15001 cggaaaccgt tgccgaaaca cccgacttca acgccaccgc agacgatttg tccgcattac
    15061 ttcaaccttc t
//
LOCUS       DAXIHS010000048        14850 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXIHS010000048 DAXIHS010000000
VERSION     DAXIHS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14850
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.048"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..289)
                     /locus_tag="ACFOF2_001290"
     CDS             complement(<1..289)
                     /locus_tag="ACFOF2_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element IS1016C family transposase"
                     /protein_id="HFC7107605.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGIL"
     gene            complement(522..2579)
                     /gene="ppk1"
                     /locus_tag="ACFOF2_001291"
     CDS             complement(522..2579)
                     /gene="ppk1"
                     /locus_tag="ACFOF2_001291"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC7107606.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPSYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRATPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2748..3191)
                     /locus_tag="ACFOF2_001292"
     CDS             complement(2748..3191)
                     /locus_tag="ACFOF2_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC7107607.1"
                     /translation="MPFRLFLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3198..3713)
                     /locus_tag="ACFOF2_001293"
     CDS             complement(3198..3713)
                     /locus_tag="ACFOF2_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC7107608.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGATDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVHEWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3980..6610
                     /gene="leuS"
                     /locus_tag="ACFOF2_001294"
     CDS             3980..6610
                     /gene="leuS"
                     /locus_tag="ACFOF2_001294"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC7107609.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFKRLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEASLVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6845..7657
                     /locus_tag="ACFOF2_001295"
     CDS             6845..7657
                     /locus_tag="ACFOF2_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC7107610.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            7650..8450
                     /locus_tag="ACFOF2_001296"
     CDS             7650..8450
                     /locus_tag="ACFOF2_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC7107611.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKG"
     gene            8455..9009
                     /locus_tag="ACFOF2_001297"
     CDS             8455..9009
                     /locus_tag="ACFOF2_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107612.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRKLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(9884..10195)
                     /locus_tag="ACFOF2_001298"
     CDS             complement(9884..10195)
                     /locus_tag="ACFOF2_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC7107613.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10192..10559)
                     /locus_tag="ACFOF2_001299"
                     /pseudo
     CDS             complement(10192..10559)
                     /locus_tag="ACFOF2_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11363..11448)
                     /locus_tag="ACFOF2_001300"
     tRNA            complement(11363..11448)
                     /locus_tag="ACFOF2_001300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11412..11414),aa:Leu,seq:gag)
     gene            complement(11462..11812)
                     /gene="secG"
                     /locus_tag="ACFOF2_001301"
     CDS             complement(11462..11812)
                     /gene="secG"
                     /locus_tag="ACFOF2_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC7107614.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(11819..12592)
                     /gene="tpiA"
                     /locus_tag="ACFOF2_001302"
     CDS             complement(11819..12592)
                     /gene="tpiA"
                     /locus_tag="ACFOF2_001302"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC7107615.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            12752..13279
                     /locus_tag="ACFOF2_001303"
     CDS             12752..13279
                     /locus_tag="ACFOF2_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC7107616.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADETDWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGT
                     VVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAA"
     gene            13381..14037
                     /locus_tag="ACFOF2_001304"
     CDS             13381..14037
                     /locus_tag="ACFOF2_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC7107617.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            14116..14439
                     /locus_tag="ACFOF2_001305"
     CDS             14116..14439
                     /locus_tag="ACFOF2_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC7107618.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
BASE COUNT         3440 a         3605 c         4054 g         3751 t
ORIGIN      
        1 tcagaatgcc gaagacaacc acttttcctg ccgcaccgcg accacgtctg cctttacgcc
       61 gtccgccgaa atcgctttcg tccggctcga cagggccctc aaaaacctca tcggcagcca
      121 aggccaaatg atggttgata accgtgcgga ttttacggta gaacagtgct gccgaattgg
      181 gatggatacc caaaatatcg gcggcagaac gggcggtaac ttccagtaca aaaaaacgga
      241 gcagttcttt ctgtactttt ttctttaatt tgcagtgcgt tatcttcata tttcgagggt
      301 aacatatctg ctaatctagt acagccccta aaatttaatc cactataatg ccgtctggaa
      361 tgcggcggct tggggttggg agcgtatagg ggaagtgctt gtgcaggaat ggcgaaggtg
      421 tgtttttgac tccggtccgc cctgtttaga ttgctgtaaa accatgccgt ctgaaacgga
      481 aaggtttcag acggcatggt ttttgattgg ggcggtgcgg gtcagcctcc gagcagtgtc
      541 cacaaatcgt tttgcaggtc ggcttcgctt tcgccctcgg cgggcgtggc gcggatatag
      601 ccgccgtcgg gctgcatcag ccacgcgtgc gtattgtctg ccaatgccat ttccaaacct
      661 tcgcggatga cgcgtttttt gagttcgggc gcggtaatcg gcgcggcggt ttcgatgcgg
      721 cggaagaagt tgcgccccat ccaatccgcg ctggagataa aggtatcgtc cgcgccgttg
      781 ttgtggaagc aatacacgcg cgcgtgttcg agctgcctgc cgatgatgga gcggacgcgg
      841 atgttttcgg acaagccttt tacacccggg cgcaaggtgc acataccgcg cacaatcaaa
      901 tcgatttgta cgcctgccgc gcttgcccga tacagggctt cgatgacggt cggttcgatg
      961 agcgaattca tcttggcggt aatccgcgcc ggtttgccgg cttttgcgtg ttcggtttcg
     1021 cgtgcgatgc ggtcgataac cattttgtgc agggtaaacg ggctttggta gagtttgttc
     1081 agccgcccgg gtttgcccaa gcctgtgatt tccataaaca atgtgttcac atcggcggtg
     1141 atttgttcgt cggcggtaat gatgccgaag tcggtgtaga tgcgcgatgt gccttggtgg
     1201 tagttacccg tgccgaggtg ggcgtaacgc ttgagtacgc cgtcttcgcg gcggatgacc
     1261 agtgccatct tggcgtggac tttgtagccg aacacgccgt acacgacgtg cgcgcccgcc
     1321 tcttcgagct gcttcgccca gttgacgttg ttggcttcgt caaaacgcgc catcagttcg
     1381 acgacgacgg ttacttgttt gccggcgagt gccgccttca tcagggcgcg gacgagttcg
     1441 gaacgcgtgc cggtgcggta aatcgtcatt ttgacggcaa gcacgtcggg gtcggcggca
     1501 gcttcgcgca tcatttcgac aacggggtcg aacgattggt aggggtggtg cagcaggatg
     1561 ggcgattggc gcaccaaatc gaatatggac gcggttttgc ccaaggcttt cagtctgccc
     1621 ggcgtgtgtg tgggaaattt caaatcgggg cggttgacta ggtcggggac ggcgttgagg
     1681 cgcacgaggt tgaccgggcc tttgacctga tagagttcgg cggcggtcag tttgaattgc
     1741 gcgagcagaa agtcgcggat gtaggaggga caggtgtcgg cgacttcgag ccgcacgccg
     1801 tcgccgtatt cgcggtcgtg cagttcgttt tgaatggcgg cgcggaggtt ttgtacgtct
     1861 tcttcgtcaa cggtcaagtc gctgtcgcgc gtgaggcgga actggtggca gcctttgacg
     1921 ttcatgcccg ggaagagttt gccgacgtgg gcgtgcagga tggaggagag gaagacgaag
     1981 ccgtgtccgc cgccacacag ttcggacggc aggggaacaa cgcgcggcag gatgcgcggt
     2041 gcctgcacaa tcgccatccc cgaaggcctg ccgaacgcgt ccgtgccgtc gagttcgacg
     2101 gcgaagttga gcgatttgtt cagcggtcgc gggaaggggt gggacgggtc gagtccgatg
     2161 ggggtcagaa tcggcagcaa ttcgcggtcg aaatagcttt cgatccactt tttctgcgcg
     2221 cccgtccagt tgcggcggcg gtaaaaatgg atgccttctt gtgccagctc gggctgaagg
     2281 acgttgttga acaggtcgta ctggtgccgt atcagggagc gcgccgcttc ggtaacgtcg
     2341 gcgatggttt cagacggcat tttgccgttg tccagcctgc gccgggggtg cagcttgttt
     2401 tcacgcttga gccacgccat gcggacttcg aaaaactcgt caagattgga cgacacgatg
     2461 cacaggaagc gcaggcgttc caaaaggggg acgtttttgt cttccgcctg tgccaacacg
     2521 cggcggttga atgccagcag gcttagttcg cggcagagga tgcggttttg ttcgggcata
     2581 aggttctccc aaagatggtt gttgttcggt cggagcgggc aggcaatgcc gtctgaaggc
     2641 ttggcggcat ctgcgccggt ttcagacggc atgacgtggc ggacagtctg tttcaggcat
     2701 cggggcaatg tgaaaacaga taccggcgcg tgcggtatcc gtctgtttca aatcacttcc
     2761 aacacaaaat aatgacgcag tttttcgtac accgcatcgc tgacgttgat gcagccgttg
     2821 gtcataatcc tgtcggacac ggacggggag gcgatgcgtt cgttccgcct ttccgacggt
     2881 atctgattcc aaacgcggtg cagggcgaaa agaaaatcac cctcctgctt gaaaccgatg
     2941 acttcgccgc cgtatccagg cttgtcggta ctgttcagcg tcagcccgaa cgtccccttg
     3001 ggcgtggcaa caccgatgag gacggggtgg cactgcctgt catcggcaaa acagagttcc
     3061 gctttggatg tgtttacgat gacttttttc ttttgaatat aagcgttgac cgcatccggt
     3121 tgcccctgtg catatacggg ggcaaccatc gataggtcgc acaatatccc taaaaacagg
     3181 cggaacggca tgtcggatta ttggcggata cgtttcggtt ttgccggcac gatttcgcgg
     3241 atgatgactt gcggctcggc tttgggtgcg ggcggcggac aggcggcatc tttcgggaag
     3301 acggggttcc agtagaagct gcgggcaaat ttgtctttat cgaaaatcac tttgtattgg
     3361 caggtagtaa catcttctac gccggacgtg ttttcagggt cgatacctac gcccggggta
     3421 tggaagtgga acaggtaatc ccattcgtgt acgccgtaca tgccttcgtc gtaatgcggg
     3481 cgacccagga ttttgtagat gtcgtctttg gtcaggccgg ggcgcatctg atccagttcg
     3541 tcataggtcg ggaatgtgcc gcgcttgttg tcgagcgtta cggaataggg tttcgggaaa
     3601 accggattgt cggtcgctcc gtcggctttg acgttgcttt tggttgcgca ggcagacaga
     3661 atgcccgccg ccaatactgc caagcccggt ttgacgattt gtttgatttt catatgcaga
     3721 atcctttttt acccgatgcc cgtctgcctt ttgttcggaa gagccgcatc aggagagttt
     3781 taaacatttg attttggttt gtaatattag cataaaaaac gtgcgtatca gtaaacgcgg
     3841 tgtatttgta cggcatacgg aatgatgcgc gtgcgaattt acgcatcctg ccggcaattt
     3901 gccgattcgc cgacatcggc aacctgttat aattcctcct ttaaattcct aacgttttca
     3961 agcgaaaaac agaatgacta tgcaagaaca atatcaaccc gccgccatcg agcctgcggc
     4021 gcagaaaaaa tgggacgacg cccgtatttt caacgtctcc gaagacgctt ccaaacccaa
     4081 atactactgc ctctccatgt tcccctaccc cagcggcaag ctgcatatgg ggcatgtgcg
     4141 caactacacc atcggcgacg tattgagccg cttcaaactt ttaaacggct tcaacgtcat
     4201 gcagcctatg ggttgggacg cgttcggcat gccggcggaa aacgcggcga tgaaaaacaa
     4261 cgtcgcccct gccgcttgga cgtacgataa catcgaatac atgaaaaccc agctcaaaag
     4321 cctgggtttt gcgattgact gggcgcgcga aaccgcaacc tgcaaacccg aatactaccg
     4381 ctgggaacaa tggctgttta ccaagctgtt tgaaaaaggc atcgtttatc gcaaaaacgg
     4441 cacggtaaac tgggacccgg tcgaccaaac cgtccttgcc aacgagcaag ttatcgacgg
     4501 acgcggttgg cgttcgggcg cgttgatcga aaaacgcgaa atcccgatgt attacttcaa
     4561 aatcaccgat tacgccgaag agctgctcaa cgacttggac aagctggaac actggcctga
     4621 acaagtcaaa accatgcagc gcaactggat cggcaaatct cgcggcatga ccgtgcgctt
     4681 tgctgtttca gacgacagca aacaaggctt ggaaggcgat tacgcgaaat tcctgcaagt
     4741 ttataccacc cgccccgaca cgctgatggg tgcgacttat gttgccgttg ccgccgagca
     4801 tccgctggca gccgccgcag ccgccgacaa acccgaattg caggcattta ttgccgaatg
     4861 caaagccggt tcggttgccg aagccgatat ggcgacgatg gagaaaaaag gcgtgccgac
     4921 cggtcgctac gtcgtcaacc cgctcaacgg cgacaagctg gaagtgtgga ttgccaacta
     4981 tgtattgtgg ggctacggcg acggcgcagt gatggcggtt ccggcgcacg acgaacgcga
     5041 ttttgagttc gccgccaaat acaatctgcc taaaaaacaa gtcattgccg tcggcgacaa
     5101 cgcattcgac gcaaaccgat ggcaagaatg gtatgccgac aaagaaaacg gcgtattggt
     5161 caacagcggc gacttggacg gcttggattt tcagacggca ttcgatgccg ttgccgccaa
     5221 gctgcaaagc caaggcgcgg gcgaaccgaa aacccaatac cgcctgcgcg actggggcat
     5281 ttcgcgccaa cgctactggg gctgtccaat tcccatcgtc cattgtgaaa aatgcggcga
     5341 cgtacccgtc ccagccgacc aactgcccgt cgttctgccc gaaaacgtcg tacccgacgg
     5401 catgggttcg ccgctggcaa aaatgcccga gttttacgaa actgcctgcc cgtgctgcgg
     5461 cggcgcggcg aaacgcgaaa ccgacaccat ggacaccttc atggaatcaa gctggtattt
     5521 cttccgctat atgtcgccca agttttcaga cggcatggta tcggcagaag ccgcgaaata
     5581 ctggggcgcg gtcgaccaat acatcggcgg tatcgaacac gcgattttgc atctcttgta
     5641 cgcgcgtttc ttcaccaaac tgatgcgcga cgaaggtttg gtcaatgttg acgaaccgtt
     5701 caaacgcctg ctcacgcaag gcatggtcgt ctgcgaaacc tactaccgcg aaaacgacaa
     5761 aggcggcaaa gactggatca accccgccga tgtcgagctg acgttcgacg acaaaggccg
     5821 ccccgtttcc gccgtcctca aagccgacgg cctgcccgtc gtcatcagcg gcacggaaaa
     5881 aatgtccaag tccaaaaaca acggcgtcga tccgcaagaa ctgattaacg cctacggcgc
     5941 ggacaccgcc cgcctgttca tgatgtttgc cgcaccgccc gaacagtccc tcgaatggag
     6001 cgacagcggc gtcgaaggcg cacaccgctt cctgcgccgc ctgtggcgca ccgtttacga
     6061 atacctgaaa caaggcggcg cggtcaaagc gttcgcaggc agccaagatg gtttgtctaa
     6121 agaactcaaa gacctgcgcc acaaactgca ttccactatc gccaaagtca gcgacgacta
     6181 cggccgccgc cagcagttca acaccgccat cgccgccgtg atggaattgc tcaaccaata
     6241 cgacaaaacc gacaccggca gcgaacaagg ccgcgccgtc gcccaagaag tattggaagc
     6301 cgccgtacgt ctgctgtggc ccatcgtgcc gcacatctgc gaaaccctgt ggagcgaatt
     6361 gaacggcgcg aaattgtggg aagcaggctg gccgacagtc gacgaagcct ccttggtcaa
     6421 atccgaaatc gaagtcatgg ttcaagtcaa cggcaaactg cgcggaaaaa tcaccgtcgc
     6481 cgccgacgca tccaaagccg acctcgaagc cgccgcactc gccaccgagg gcgcagtgaa
     6541 attcatggaa ggcaagcccg ccaagaaaat catcgtcgtg ccgggcagac tggtgaacat
     6601 cgtcgtctaa ccgcttttaa ggtttagcca cacggataaa ggccgtctga aacttggaaa
     6661 cagggtttca gacggccttt ttaaggcaga tggagttgat aagtccaata acatattgga
     6721 atggaaaggt tctattggat ttagactcac tctacacacg cccccaaata taaatccatt
     6781 gtattcctca cgatgttttg gcacaattcc aagccaatca accacgcttg tactcggcat
     6841 cctaatggct tcaaaacctt ttctaaaatg ggcaggcggc aaacataaac ttgtcccctt
     6901 tatcgaacac aatctaccta ctcccacacg aaaacgcctg attgaaccct tttgcggttc
     6961 tgccgctttg tctcttgcat tggatttcga acaatatctg ctcaatgata ttaacgctga
     7021 cttaatcggc ttattccgta tcctaaaaga agaaaaatcc ggattcatcg actacgcccg
     7081 ctctttcttt atttccgaaa acaatagcga ttcaagattc tatgaattgc gggaacaatt
     7141 caacttcagc aaaaatctgc acgaacgctc ggcactattt atttatttga accgtcatgc
     7201 ttttaacggt ttatgccgtt acaacagcaa aggggtattc aatgtgccgt tcggacgcta
     7261 caaatcgccg tactttcccc aacaagaaat ggaaggtttc attcagaaat ctgacagagt
     7321 tgaattaatg tgcggagatt ttcaaatgat tctatctcag acagacaata ccgatacggt
     7381 ttattgcgat cccccttacg cgcctttaag cccaaccgct tcatttacca cttatgccaa
     7441 agaaggtttc aatttagaca atcaaatccg tcttgcccag tcagcccaac aaattgcgcc
     7501 cgattcccaa ggcgttttga tttcaaatca tgatactgaa ttcacaaggg atatttattc
     7561 ttttgctatt ctgaaaacaa ttgaagtaca acgcaatatt gccgcaaaag gcagcagccg
     7621 taaaaaagtc ggagaattat tggcaattta tgattgattt tgacaaaccg gctgaagaag
     7681 acgccattta tcaaagtcgt ttgaaaaaaa tcgtttcaga cgaccttgtc ctctacaacg
     7741 aaaattccct taacgtcatg cggaagatat tggaaaaata tccaaacggc tgttttgata
     7801 tgattttcgc agacccgcct tactttcttt ccaatgacgg cttcagttgt caaaacggac
     7861 aaatggtttc cgtcaataag ggcaactggg ataaatccaa aggaatggcg gcagatttgg
     7921 aattctacga agaatggctc cgactgtgtt acgccttatt aaaaccaaac ggcacaattt
     7981 gggtttgcgg cacattccat aatatttatt taatcggcta cctgatgcaa actatcggat
     8041 accatatttt gaacaatatt acttgggaaa aacccaatcc tccccctaat ttgtcctgcc
     8101 gtttttttac ccattcgaca gaaacaatct tatgggcaaa gaaaaataaa aaagccaaac
     8161 acacgtttca ttatgaaatg atgaaagcac aaaatgatgg caaacagatg aaatgtgttt
     8221 ggacatttcc tcctccaaac aaagccgaaa aaacattcgg caaacatccg acacaaaaac
     8281 cactcccctt gcttgaacgc tgcatactct cagcttcaaa catcggagat ttaatttttg
     8341 accctttcat gggcagcggc acaacaggcg ttgccgcctt aaaacatggt cggaaatttt
     8401 gtggttgcga actggaagaa gatttttttg aattagcaaa aaaaggttag aaaaatgatt
     8461 attggcggaa tcggtggtgc aaggacacaa acaggactca gattcgaaga acgtacagac
     8521 ttacgaaagt tgtttgaaga aattcccggg tacgacttaa gaaaaacata tgataatgcg
     8581 ggttatgaag tttggttcaa tggagaattg aaggcttatt gttttaaaaa atatgagttt
     8641 taccgatttt tgaaaagacc gggatacagt attaattgga aagaccatct gtctaaaaga
     8701 ttactacccg acaatggctt atttatcatc atccgtgata ccttatttat tattgagatc
     8761 aaattccaac aaactcccgg ttcagtagat gagaaattac aaacttgcga ttttaagaga
     8821 aaacaatata caaaattggt tcactcttta ggttggcggg ttgaatatgt ctatgttttg
     8881 aatgattggt ttacaaagcc ggaatataaa gatgttttag attatattat cagtgttaac
     8941 tgccattacc aatttaacac gattccctta aggtggtttg gactgcctga tggtgaaaca
     9001 aatgaataaa taccatattt aaaacacagc cgttcaaggt cgtctgaata tcatttcaga
     9061 cgacctttca ccaatccccg ccgttttcca tctttctgct attgtgataa agtagcccga
     9121 ccttttgttc aaaatacgcg gattccgcga cactgaatgc agcatctgtc aaagattatt
     9181 tgaaaacggt cgagttagac aagtctgccg acaatgtcga taccacatcc aaaactatcc
     9241 gcagggttta ggcggtattc cagagtacgg tgtcttatgc tgatgctgta aacaatgccc
     9301 gtatagaaaa tctaaaacgg ttgttgccga ccgttatgcc ggtttatgag caaagtgtca
     9361 gaaataaggg gcgcgttaat atagtggatt aaatttaaat caggacaagg cgacgaagcc
     9421 gcagacagta cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttaatcca
     9481 ctataaaaaa cgtcggcgtt aagggaaggg gatcgagaat ttgagccgtt gtttcaaaat
     9541 gccgtctgaa atcgtttgag attcagacgg catttaaaca gttctgcgcc cgcccgtttt
     9601 gccttcaagc ccgcgccgct taaatccaat cccccctgca acgggcgcaa aaaaatctcc
     9661 gtgcctcgcg gtcggggatg tttcgggttt cacgcccgtc tgcgccgtgc cttgctgtcg
     9721 ggctatgccg tctgcaccgc cgccgtttcg gcttggtttg atgcggtcgg gcatttatgc
     9781 ggctacgccc tgatattcag ttcggcggtc ttgcctgttt tggcaggcat attcagcagc
     9841 gcgtctattg tgaacttgct gattttgccg tttgccaagt cggctacgca atggcttcga
     9901 tacccttgtt tttgacgatg gtcagcagct tcaacgccgc gccgctgggc tttttaacgc
     9961 cccgctccca agccgaaacg tgattttttc ccacgttgag atagatggcg aaagcggctt
    10021 gcgatagtgc ctccttctcc ctgattgcct tgatgtctcc gccgctcaac ggtttgattt
    10081 cggtcaggca ggacttgtca aagccgcgca tagtcttttt gtcgattgcg ccgatgttgt
    10141 aagccccttc catcatttca tgaatggcgg ctaatgcctc gcttttgtat ttcatgattc
    10201 tatctccgtg ataatgccgt cttctttcaa agccgccagc tctgcccgcg tgtatttcag
    10261 ataatatgcg gcggcttttc ggtaaacgtc cagttcttta tccgaaatgt tttccctgtc
    10321 gttcttggca aaggcgtaaa cgaaaatgcc ctatctgcct gtttgaacaa tatcaggctg
    10381 cgatagccgc cgcttctgcc ttgtccttgc cttgctatgc gctgcttaat cacaccgccg
    10441 cctaaatctg cgtctatcag cccgttatcc gcccgctcta ccgcttccag cagctcggaa
    10501 tcgttgattt tgtgcttctt ggcaaatttc actatccatt ggtttttgaa tatccgcatt
    10561 gtgtagccgt tgattagtga tacttggaat tataccagag acagacaaca cggcaagctg
    10621 ccttttttat gccccgcaat ttctccgttt atgccgcctg aaacactggc ggctaacctg
    10681 attatttttt aatcaatcgc ttttaatggc tcaaattgcg tttttagcgc gtttcgctgt
    10741 cttcctatgt ccccatatat aaccaagtgg tttaaccgcc attcctgctc aaaataggcg
    10801 cggttccgtg cgttttggct tttcattccg ctacgctccc actgccaaga cggtgaaaaa
    10861 ccggaaaacc ccaaaagttg ttaccgattc gttaccattt gttaccgcat ttgttaccaa
    10921 ttttttatct ttttcttatt ttatagtgaa ttaaatttaa accggtacgg caaggcgagg
    10981 caacgccgta ccggtttaaa tttaatccac tatagtatag gttttttcca aaaacccaaa
    11041 aaaaaaccgc tctgcatctt ggacggtttc cccctcattc cctacggtgc gtcagtattc
    11101 cccgcaagct atttcccgct tggcattcct gatttggtgg tcgttcagct ttatttttac
    11161 ggtattttct cgcggtattt tcggatgctg taaaaaatca ggttagttca attagggtgg
    11221 attggacggg attgtacggt caggaagggg gacggcagga taacaatcag cctgaaagcc
    11281 ttgtattgat tgggattggt tgacggcatt agacaggatt aaacgaaaaa aaacgcttag
    11341 aatttctaag cgttttgtgt gttggtgccg acagcgagat ttgaactcgc acagcctacg
    11401 gccactaccc cctcaagata gcgtgtctac caatttcacc atgtcggcat ttgaaaaact
    11461 gttatttctg ctgctgagga acaggggcag aaggttcggt attgcttacg ggtttgggtg
    11521 cttgctgagt ttgttgtacg ttgctgaagt ccaaaccgtg ttttgttgtg tgggtgtgaa
    11581 tatacaccat agccatgcag gttgcaaaga aaaatgttgc tgcaacggcg gtcgagcggc
    11641 tgaggaagtt agcgttgccg gcagagccga atacgccttg cgcgctgccg cttcccgatc
    11701 cgaaagtcgc gccggcatcc gcgcctttgc cgtgttggag caatactaac acgatgacgg
    11761 ccaaagcgga aattatatta acaatccaaa ttagggtttt gaaggcttcc atatttttct
    11821 acgcattttg tgcggcactg atgatggcgg taaaggagtc gtacgacaat gacgcgccgc
    11881 cgacgagtgc gccgtccaca taaggtactg cgaagatgtc ggccgcgttg tccgctttca
    11941 cacttccgcc gtaaaggacg cggattttaa catcgcttcc gcacaaagac aagatttctt
    12001 tgtagatgaa tgcgtgcata tcggcaatct gttcgacggt ggcgactttg ccggtgccga
    12061 tcgcccagac gggttcgtag gcgacggcga tgtttttggt atccagccct tgcaggatgg
    12121 aaagctgatg ggcgatgact tcgtgttctt tgccggcttc gcgctcttcg aggctttcgc
    12181 cgacgcacaa taacgggatg agtccgacgt tgaggacgtt ttccattttg cggcgttgga
    12241 tttcgttttt ttcgccgaaa taaaggctgc gttcggagtg tccgatgagg acgatgtctg
    12301 tgccggtgtc ggcgagcatt tcggcggaca cttcgccggt gtacgcgccg ttgtcgggga
    12361 agcggctcac gtcttgggcg caggtgagga tgcggttgtt taagacgatt tgcatggcgt
    12421 tgtgcagttg cagcaggtaa acggtcgggg cggcgagtcc gatgaggacg cgttcggcgg
    12481 tggggaggat gcggaagcgg tgcatgagcg aattgttgtt ctggagccgg ccgttcattt
    12541 tccagttgcc gatgacccat ttttgatccc acattccgat ttggtgatac atcttttttg
    12601 ctccgtgtcg tgttttttct gtctgccgcg tgtggcgcgt tgcaatgtga agtttagtgg
    12661 atatgcggcg ggttcgcaac ttggggcggg cggctgcggg ggcggtttgg aatgttgttt
    12721 cgggcaggtt gttttataat ggccgcctga tatgtatgca actataggag atgtgatgca
    12781 cgcgcttcat ttttcggctt cggacaaggc cgcgctttat cgggaggtgt tgccgcagat
    12841 tgagtctgtg gtggcggacg agacggattg ggtggcgaat ttggcgaaca cggcggcggt
    12901 tttgaaggag gcgttcggtt ggttttgggt gggtttttat ttggtcgata cgcgttcgga
    12961 cgaattggtt ttggcaccgt ttcaggggcc tttggcgtgt acgcggattc cgttcggtcg
    13021 cggggtgtgc ggtcaggctt gggcgaaggg cgggacggtg gtcgtcgggg atgtggacgc
    13081 gcatcccgac catattgcct gttcgtcttt gtcgcgttcg gagattgtcg tgccgctgtt
    13141 ttcagacggc cgctgtatcg gcgtgttgga cgcggacagc gaacatttgg cgcagtttga
    13201 tgagacagat gctttgtatt tgggcgaact ggcgaagatt ttggagaagc ggtttgaggc
    13261 ttcgcgccag gcggcttgag tgatggaaag gggcgggcgg cgcgtgccga agttggcgcg
    13321 gcgggagtgt ggttttataa tgcctgccat tgttgaaaca attatttgac ggagcactaa
    13381 atggattttg aaaaagcgcg gttcaatatg gtcgaacagc aaatccgtcc ttgggatgta
    13441 ttggattttg acgttttgga cgctttggag gagattccgc gcgagctttt tgtcgatgag
    13501 gatttgcagg gtttggcgta tgcggatatg gcgctgccgc ttgccaacgg tcataagatg
    13561 ctggagccga aagtcgtggc gcggctggcg cagggcttga agctgacgaa aaacgatacg
    13621 gttttggaaa tcggcacggg ttcgggctat gcgaccgcgc ttttggcaaa attggctggt
    13681 cgtgtggtgt cggacgacat cgatgccgaa cagcaaaacc gcgccaaagc ggtgttggac
    13741 ggcttgtctt tggaaaacat tgattatgtg caaaacaacg ggttgaccga actttccgca
    13801 ggtgcgccgt ttgatgcggt ttatgtcggc ggtgcggtaa accttgtgcc tgaagtgttg
    13861 aaagaacagt tgaaagatgg cggacgcatg gtggttatcg taggacgcaa gcctgttcaa
    13921 cgcgcgcttt tgattacgcg caggggcgat gtgtttgaag agaaggtgct gttcgatacc
    13981 ttggtggcgc atttggatga taaggatgcc gatccgtttg gtggttttca gttttgatgt
    14041 tcggattgcg atgccgtctg aaagcgggtt tggggcttca gacggcattt tgcttggttt
    14101 tttttggggg gtgcgatgga tattgtgcaa cttgggtcgg cggaattgaa ggcgtggatg
    14161 gacgaagggc ggatgttttg tttgttggac gtgcgtacgg atgaagaaac ggcggtttgt
    14221 tcgctgccaa atgcgctgca tatcccgatg aatctgattc cgctgcggca gaacgagctg
    14281 ccggatgatg tgccgcttgt ggtgtattgc caccacggta tccgcagcct gcatacggcg
    14341 atgtatctgg cggacgcggg ttttgaaaac ctgtacaacc ttcagggcgg catcgatgca
    14401 tgggcggccg agattgacgg caacatgatg cgttattgag gcaggcattt cagacggcat
    14461 tccttaaatg cgtgtacctg tggtgttcca aaaggtcgtc tgaaagtgca gcttctgcga
    14521 agctaaagcg cggtatcaac gaagttaaaa tttgcctgaa ccttaaaggc agcctgcacc
    14581 ccaatcccct ctcccgtggg agagggctag gacgaggctg gcaaactgaa agtttgcttg
    14641 ggcggcattt tcaatgtgca ggctgctttt atagtggatt aacaaaaatc aggacaaggc
    14701 gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgct tgagcacctt
    14761 agagaatcgt tctctttgag ctaaggcgag gcaacaccgt actggttttt gttaatccac
    14821 tatagcttcg cagaaactcc gttttcagac
//
LOCUS       DAXIHS010000049        14846 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXIHS010000049 DAXIHS010000000
VERSION     DAXIHS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.049"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(48..611)
                     /gene="orn"
                     /locus_tag="ACFOF2_001306"
     CDS             complement(48..611)
                     /gene="orn"
                     /locus_tag="ACFOF2_001306"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC7107619.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(629..1516)
                     /gene="prmA"
                     /locus_tag="ACFOF2_001307"
     CDS             complement(629..1516)
                     /gene="prmA"
                     /locus_tag="ACFOF2_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC7107620.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            complement(1777..3138)
                     /gene="accC"
                     /locus_tag="ACFOF2_001308"
     CDS             complement(1777..3138)
                     /gene="accC"
                     /locus_tag="ACFOF2_001308"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC7107621.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(3252..3704)
                     /gene="accB"
                     /locus_tag="ACFOF2_001309"
     CDS             complement(3252..3704)
                     /gene="accB"
                     /locus_tag="ACFOF2_001309"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC7107622.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(3686..3943)
                     /locus_tag="ACFOF2_001310"
     CDS             complement(3686..3943)
                     /locus_tag="ACFOF2_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC7107623.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            4060..5100
                     /gene="queA"
                     /locus_tag="ACFOF2_001311"
     CDS             4060..5100
                     /gene="queA"
                     /locus_tag="ACFOF2_001311"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC7107624.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(5574..6152)
                     /locus_tag="ACFOF2_001312"
     CDS             complement(5574..6152)
                     /locus_tag="ACFOF2_001312"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC7107625.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            6391..7290
                     /gene="crgA"
                     /locus_tag="ACFOF2_001313"
     CDS             6391..7290
                     /gene="crgA"
                     /locus_tag="ACFOF2_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC7107626.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(7518..10733)
                     /gene="carB"
                     /locus_tag="ACFOF2_001314"
     CDS             complement(7518..10733)
                     /gene="carB"
                     /locus_tag="ACFOF2_001314"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC7107627.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(10773..11384)
                     /locus_tag="ACFOF2_001315"
     CDS             complement(10773..11384)
                     /locus_tag="ACFOF2_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107628.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(12232..12621)
                     /locus_tag="ACFOF2_001316"
     CDS             complement(12232..12621)
                     /locus_tag="ACFOF2_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC7107629.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(13194..13319)
                     /locus_tag="ACFOF2_001317"
                     /pseudo
     CDS             complement(13194..13319)
                     /locus_tag="ACFOF2_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(13365..14498)
                     /gene="carA"
                     /locus_tag="ACFOF2_001318"
     CDS             complement(13365..14498)
                     /gene="carA"
                     /locus_tag="ACFOF2_001318"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC7107630.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         3180 a         3708 c         4134 g         3824 t
ORIGIN      
        1 gccgtctgaa atgcaggatt tgcgcttcag acggcattgt ttgtttctta ttgcgcttcg
       61 gctctcgggg cggaaatcag aaagtgttcg cggtagtggc gcatttcttc gatgctctcc
      121 aaaatgtcgt ccaatgcctt gtgcgaaccg cgtttgacca cgcttttggc aacgggcgga
      181 ttccagcgtt tggcaagctc tttgagcgtg gaaacgtcga ggttgcggta gtggaagtag
      241 ttttccagtt tcggcatata tttgaccata aaacgccggt cttggtggat ggagttgccg
      301 cacatcggcg tggcgcgttc gggtacccat tccgacataa agtccagcag tttctgttcg
      361 acttcggctt cggtatgcga cgattcgcgt acgcgctgcg tcagccccgt cctaccgtgt
      421 gtggcggtgt tccactcgtc catattgtcg agcagctcgt cgctttggtg gatggcgtaa
      481 acttcggatt gcgccaatac attcaaatcc gagtcggtaa taatcatcgc gacttcgata
      541 atgcggtcgg tttcgggatt aagccccgtc atttccatat cgagccagca taggttgttt
      601 ttatcctgca tgatgtttcc tttgcttgtc agcgttttgt cccgcttaat cgcgcccatc
      661 cttcttcggt ttccgcagga tcgagatcga accattggct gtaaatgccg ctgagttctt
      721 cgatttgttc gtccaacaaa ccggataaca cgatgcggcc gccttgtttg gtgcgggcgg
      781 caagcatttc gccgagcata cgtaaagggt tggcgaggat gttggcaaca actacgtcga
      841 attgcccttg aggcaggccg tcgggcagga agaattgtgc atcgacgttg ttctgttcgg
      901 cgttgtcctt gccggcgcgg acggcttgtt cgtcaatatc gacgccgacg gcagaacctg
      961 cgccgagttt gagggcagcg atggtcagga tgcccgaacc gcagccgtag tcgaggacgc
     1021 tttcgccgtt tttgagttgc gtatccagcc atttgaggca gaggcgcgtg gttgggtggc
     1081 tgccagtgcc gaaggcgagg ccgggatcga ggcgtaggtt gacggcagtg ccttcggggg
     1141 cttcgtgcca agagggggta atccacaggc ggtcggaaat tcggatgggg tcgaattgcg
     1201 attgcgtgag acgcacccag tcttggtctt cgatggtttc gccggtgtat gccaagtctt
     1261 ttaacccgca ttcttgtgcg gcgatttgga tgatggcggc ggcttcgtcg tgttcgccga
     1321 acagggcgat gactttgctc tgctgccaga tttgttcggc gggcataccg ggttcgccga
     1381 aaatcgcctg ttcgttttgc gtgccggcgt aggcatcttc gatggcggcg gagagtgcgc
     1441 cgtgttccat cagcgcgtcg gcgaggcgtt cggcgacggc atcgttgacg ttgacggtga
     1501 tttgttggta gggcataacg ggctttcttg gaaatatagt ggattgaatt taaatcagga
     1561 caaggcgacg aagccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag
     1621 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttaaagtt
     1681 aatccactat atcgaaatcg gtttcagacg gcgcggggca agggaaatgc cgtctgaacg
     1741 cggacgggcg gcttcagacg gcatatcggc ggcggtttac ttgtcctgtt tggctttgcg
     1801 atcttccagc cagtgttcca aatagtggat gctgacgccg cctttttgga aacccgcatc
     1861 ggcgaacagg tcgcggtgaa gcggcgtatt ggttttgatg ccggttaccg ccagctcggc
     1921 gagtgcgacg cgcattttcg ccattgcctg ttcacgcgtt ttgccgtgta cgcagatttt
     1981 gccgatcagg ctgtcgtagt acggtgggat gcggtagcct tggtaaatgt ggctgtccac
     2041 gcggataccg aagccgccgg gcaggtggca gctttcaatc gggcccgggc tggggatgaa
     2101 gttgtacggg tcttcggcat tgatgcggca ctcaaacgcg tggccttcga cttgaatatc
     2161 cttttgtttg tattgcaaag gcaggccggc ggcgatgcgg agttgctcct gcacgatgtc
     2221 cacgccggta atcagctcgg taaccggatg ctcaacctga acgcgcgtat tcatctcgat
     2281 aaagaaaaat tcgccatctt cgtacaggaa ttcaaacgta cctgcgccac gatagccgat
     2341 gcgtttgcag gcgtcggcac aggcgttgcc gattttggcg cgttcttttt cagtgatgaa
     2401 cggagccggt gcttcctcga tgactttttg gtggcggcgt tgcagcgaac agtcgcgctc
     2461 ggcaaggtag atggcgttgc cgtgttcgtc ggcaatcact tggatttcga cgtggcgcgg
     2521 acgttgcaga tagcgttcca tgtaaaccat cgggttgccg aatgccgcgc ctgcttctgc
     2581 cttggtcatt tcgacagatt ggaggaggtc ttcttttttc tcgaccacgc gcataccgcg
     2641 accgccgccg ccgccagagg ctttgataat gacgggataa ccgactttat cggcgatttt
     2701 gaggatttct tcgccgtcgt cgggcaatgc gccgtcagaa ccggggacgc agggtacgcc
     2761 tgccgctatc atcgcgtgtt tggcggagac tttgtcgccc atcaggcgga tggtgtcggg
     2821 tttcgggccg ataaaggtaa agccggactg ctcgacctgt tcggcgaaat cggcgttttc
     2881 ggcaaggaaa ccgtagcccg gatggacagc gtccgcgcag cttacttcgg cggcggcgat
     2941 aatggcgggg acgttaaggt agctttgcgc ggaagcggca gggccgatgc acacggattc
     3001 gtcggcgagt ttgacgtgca ggctgtcttt gtcggcctcg gaatgcacgg cgacggtggc
     3061 aatgcccatt tcgcggcagg cacggagtac gcgtaatgcg atttcgcctc ggttggcgat
     3121 taaaactttt ttcagcatga tgactgttcc tgtggttccg gcaacccgtc cgtaaaaaaa
     3181 aaggtgcggg aacgccgaag ggggaaagca tttcagacgg catcggaagt ttatgccgtc
     3241 tgaaaacagg attatccgat gatgaagagc ggttcgccga attcgacggg cgtaccgttt
     3301 tcgaccagaa tttctttgac cgtgccggat ttttcggctt cgatttcgtt catcagcttc
     3361 atcgcttcga tgatgcacag cgtgtcgccg gctttaacct gttggccgac ttcgacaaaa
     3421 ggcgcggcat tcgggccggg tgcgcggtag aacgtgccga ccataggcga tttttgcgcg
     3481 tcggacaaat cgcgggcggc aggtgcggcg ggcgcggatg ccgcaacggg tgcggcggca
     3541 ggcgttacgg ccggcgcggc ggcaggtacg ggcgcggcgt aaacgggtgc ggcggcgatg
     3601 gttcgggtga tgcggacttt ttcctcgcct tcggttactt cgatttcggc gatacccgat
     3661 tcttcaacca aatcaatcag tttttttaat ttgcgcaaat ccatttctgt tcctttaaag
     3721 gctgccggtg ttccggcggg ttgttgcgtt ggtttttcag aaaacagccg gcttgcggca
     3781 acggcgcaaa ccgggcggaa tggtgttcgg atacggtttt cctgtggtgg aaaacaaccg
     3841 tgagaatgtg tttattttcc cgaagttgtc ggtaaatgtc cagtaaaata tcaaaaaacg
     3901 gcggtttttg ggggaaatgt gcaggaagct tgattttgcg cacaagatgc cccgggtcaa
     3961 gagtgtttat tctaatctgt tggtttttcg gacaaagatg ccgtctgaaa aagggctaaa
     4021 gtgcgtataa tggcggcttg cccgaacgag agtgtaaaaa tggatatttc agattttgat
     4081 tttaccctgc ccgaaaagct gattgcccag catccgcccg aagtgcgcgg cagcagccgc
     4141 cttttggttg cgttgcccga tatgccgctg caagaccggg tgttcggcga tttgccggat
     4201 tatgtcgagg cgggcgacgt tttggtgttc aacaacacca aagtcatgaa ggcgcggctg
     4261 tttgggcaga aagacagcgg cggcaggatc gaagccctga ttgagcgcgt gttggacaac
     4321 cataccgcac tggcgcacat ccgttcgtcc aagtccccca agcccggtat ggggctggtg
     4381 tttgaaggcg gcatccgtgc cgtgatggtc gggcgtgagg gcgaactgtt ctgcctgcgt
     4441 tttgaaggcg gtcaaaccgt ttacgaactt ttggaacaga acggccattt gcctctgccg
     4501 ccttatatcg aacgtgccgc cgacgcggac gacgacagcc gttatcaaac cgtttatgcc
     4561 aaatatcagg gcgcggtcgc cgcgccgacg gcgggcctgc attttacgga agaacttttg
     4621 caccgtctga aagacaaagg cgcggtaacc gcagaagtaa ctctgcacgt cggtgcgggg
     4681 acattccaac ccgtgcgtgt ggaaaaaatc gaagaacaca aaatgcacag cgaatggttt
     4741 gaagtgccgt ctgaaaccgc cgccgccgtt gaggcggcaa aagcccgggg gaacaaagtc
     4801 tgggcggtcg gcacgacttc catgcgcgcc ctcgaatctg ccgcgcgtgc gacgggtcgt
     4861 ctgaaagcgg ggcagggcga caccgatatt ttcatcacgc cgggctaccg ttttaatgtt
     4921 gccgacaggc tggttaccaa tttccatctg ccgaaatcga cgctgctgat gcttgtcagc
     4981 gcgttttcgg gtatgggtca tatccgcgcc gcgtatcgtt atgcggtcga acgcgaatac
     5041 cgtttcttca gctacggcga tgcgatggtt ttggggcgga acgaaggggt ggtacgttag
     5101 aacagcctca agtctattgg gggaagacaa gtgccgtctg aacggatgtt cggacggcat
     5161 tcttttgttg atttgaatga aaggaactgc atgacatata gtggattaaa tttaaatcag
     5221 gacaaggcaa cgaagccgta gacagtacaa atagtacggc aaggcgaggc aacgctgtac
     5281 tggtttttgt taatccacta tagattcagg cgcgagaggc gagaaactca gtcattgttg
     5341 atgacgcgca atacgttaaa attcattccg ttttggtcgt ataaacatat tacatacgcc
     5401 atttttttgt attcaaggct gcctgaaaac agtgggcaag aatatctaac ccaacgaaaa
     5461 gctgaaaaag caacaaaaaa taatctgcac cttagtcggt tggttagtcc aaccttaggg
     5521 gtgcagacca ttcaatcttt caggctgcct ttgtttgttt ataagataaa tttttagccg
     5581 aacacttttt ccaagtgtgc ctgataatct gccaagtatt tttccacttg tggattttta
     5641 accacatcgt tacataagaa tgtcggcagg cgggtcatac ccaaaaactc gttggctttg
     5701 tggaagtgca tatacaaaac atcaacgcct ttgccttcaa agaaatcgcc ttcgcgggta
     5761 aacgcctcaa tcggcgcatt ccaagtcagt gaaagcatat gttttttgcc ttgcaacaag
     5821 ccgcctgtgc cgtagccctc agtcggattg acgcggtgtc tgccgtcgct ttggtagagt
     5881 ttgccgtgtc cagcggttaa tacttcgtct atgtattttt tcactgtcca aggctcgtgc
     5941 atccaccagc ccggcatctg ccaaatcaca gcatccatcc aaacgaattt ttcgatttct
     6001 gcctcaacat catagccggc atcaatcacg gtttcttgaa cattgtgtcc gagcgcggtc
     6061 aaaacttctt tcgctttttt gtgaagcgtg cggtttaacc cgccgtgaga atgtccgaac
     6121 gctttgccgc cgtctaataa taaaatattc atttgttacc tcgtttgtga attgatgaat
     6181 gtattgtgcc gttttacttt ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc
     6241 aaatgaaata atcagatgtt ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc
     6301 cgtcctgatt cggaaggggt tacacccctc ccaaataaag tctgatcctg ccgccctaaa
     6361 gggcggggtt ccaaccgaaa aggaaacacg atgaaaacca attcagaaga actgaccgta
     6421 tttgttcaag tggtggaaag cggcagcttc agccgtgcgg cggagcagtt ggcgatggca
     6481 aattctgccg taagccgcat cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc
     6541 aaccgcacca cgcggcaact cagtctgacg gaagaaggag cgcaatattt ccgccgcgcg
     6601 cagagaatcc tgcaagaaat ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata
     6661 ccgcaaggcg tgttgcgcgt ggattccgcg atgccgatgg tgctgcatct gctggcgccg
     6721 ctggcaacaa aattcaacga acgctatccg catatccgac tttcgctcgt ttcttccgaa
     6781 ggctatatca atctgattga acgcaaagtc gatattgcct tacgggccgg agaattggac
     6841 gattccgggc tgcgtgcacg ccatctgttt gacagccgct tccgcgtaat cgccagtcct
     6901 gaatacctgg caaaacacgg cacgccgcaa tctacagaag agcttgccgg ccaccaatgt
     6961 ttaggcttca ccgaacccgg ttctctaaat acatgggcgg ttttagatgc gcagggaaat
     7021 ccctataaga tttcaccgca ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc
     7081 ctttcaggtt gcggtattgc ttgcttatca gattttttgg ttgacaacga catcgctgaa
     7141 ggaaagttaa ttcccctgct cgccgaacaa acctccgata aaacacaccc ctttaatgct
     7201 gtttattaca gcgataaagc cgtcaatctc cgcttacgcg tatttttgga ttttttagtg
     7261 gaggaactgg gaaacaatct ctgtggataa ttctataaaa acctgccata aaattgtttt
     7321 tactcactta ataactttga gaaaaaataa ataagatgat gtatagccgc ggcaatggca
     7381 gtctatatgg ggagcgtgat agggctgttc cttatgtacg gcttgtgtgt ggcaacatcg
     7441 gctttccaaa tgacaaaatg ccgtctgaaa ccgtaaaaaa aaaggtttca gacggcattt
     7501 tcaacctgat tcaggcatca gttgcggttt ttcaaacgcc cgtgcagttc ttgaacgctg
     7561 tacacgccca agtggtcgcg gcttttcgcg ccttcgctca tcgcttcgcc gccggcggtg
     7621 gtggtgtatt gcggcacgcg ttgctgcaat gcgctttggc ggatgatgtg gctgtcggac
     7681 acggattgcg gatcgctgga aacggtattc acgaccagtg cgatttcacc gtttttcagc
     7741 gcgtcgccga tgtgcgggcg gccttcgggg actttattga tggtttggac aatcagcccg
     7801 tgttcggtca ggtattgcgc cgtgccgcgc gtggcgcaga tgccgtagcc taaggcttgg
     7861 aagtttttgg cggttttaat gacgcgttct ttgtcttctt cgcgcacgga gaggaagatt
     7921 ttgccggtcg ggttgaggcg ttcgcctgcg ccgagttggg ctttgtagta ggcttcgccg
     7981 aagcttgcgc ccacgcccat gacttcgccg gtggagcgca tttccggacc caaaatcgta
     8041 tccacgcccg ggaatttgat gaaggggaac acggcttctt taacggcata gaaatcaggg
     8101 acgacttctt tttccacgcc ttgttctttc agggaaatgc ctgccataca gcgtgcgccg
     8161 actttggcga gcggcacgcc ggtggctttg gagacgaagg ggacggtgcg gctggcgcgc
     8221 gggttcactt ccaatacgaa cactacgccg tcctgcacgg caaactgcac gttcatcagt
     8281 ccgaccacgc ccagcgcgta cgccatcgct ttggtttggc ggcggatttc gtcttggatt
     8341 tcttcgctta aggagtaggg cggcagcgag cagccggagt cgccggagtg gatgcccgcc
     8401 tgttcgacgt gctgcatgat gccgccgata accacatctt tgccgtctga aacgcagtcc
     8461 acatcgactt caatcgcgtt gttcaggaag aagtcgagca acacggggct gtcttcggaa
     8521 acctgcacgg cttcgcgcat atatttttgc aactgctcgg cagagtggac aatctgcatc
     8581 gcgcgtccgc ccaaaacata agacgggcgc acgaccagcg gataaccgat ttcttcggct
     8641 ttgacgagtg cttcttcttc gttgtgggcg atgcggttgg gcggctggcg caggcctaag
     8701 tcgttcaaca ctttttggaa gcgttcgcgg tcttcggcgg catcgatgct gtcggcggac
     8761 gtgccgatga tgttcacgcc gttttcaacc agcgcgttgg cgagtttgag cggggtttga
     8821 ccgccgtaat gcacaatcac gccccacggg ttttcggtgc ggacgatttc caacacgtct
     8881 tccaatgtca gcggctcgaa atagaggcgg tcgctggtgt cgaagtcggt ggacacggtt
     8941 tctgggttgc agttgaccat aatcgtttca aaaccggatt cgcgcagggc gagcgcggcg
     9001 tgaacgcagc agtagtcaaa ctcgatgccc tgaccgatgc ggttcgggcc gccgccgaga
     9061 atcatcactt ttttgcggtc ggaaggacgc gcttcgcatt cctcttcgta agtggaatag
     9121 aggtaggcgg tttcggtggc gaactcggcg gcacaggtgt cgacgcgttt gtaaaccgga
     9181 tgcagcttca gcgcgtagcg gtgttcgcga acttcttttt cgcttacgtt caacaattgt
     9241 gccaaacgtt tgtcggagaa gcctttgcgt ttcagacggc gtagggcggc gaaatccaaa
     9301 tcactcaaaa tgccgtctga aaccgctttt tcttccttca tcaagtcttc gatttgcgcc
     9361 aggaaccaag ggtcgatggc gcagatttcg tggatttctt ccagcgtgaa gccggcgcgg
     9421 aacgcgtctg ccacaaacag catacgctca gggccggggt tcgccagttc gcggcggatt
     9481 tccgctttgt cggagcttct cggattgaag ccgcacaagc cggtttccaa gccgcgcaag
     9541 gctttttgga agctttcctg aatcgtgcgg cccatcgcca tcacttcacc caccgatttc
     9601 atctgtgtgg tcaggcggtc gtctgcggca gggaattttt caaacgcgaa acgcgggatt
     9661 ttggttacga catagtcgat ggacggctcg aacgacgcgg gggttttgcc gccggtgatg
     9721 tcgttgcgca actcgtccag cgtaaagccg accgccagct tggccgccac cttcgcaatc
     9781 gggaagcccg tcgctttgga agccagcgcg gacgaacggc tcacgcgcgg gttcatctca
     9841 atcacaatca tctcgccgtt ttcagggttc atcgcaaact gcacgtttga gccgcccgtg
     9901 tccacgccga tttcgcgcaa taccgccaac gaagcgttgc gcatgatttg gtattccttg
     9961 tccgtcagcg tttgcgccgg cgcgaccgta atcgagtcgc ccgtatgcac gcccatcggg
    10021 tcgaagtttt caatcgagca gatgatgatg cagttgtcgt tcttgtcgcg cactacttcc
    10081 atctcgtact ctttccagcc gagaacggac tgctcaatca gcagctcgtg cgtgggcgag
    10141 gcatcgaaac cgcgttcgca aatcgccaaa aactcgtctt tattgtaggc aatgccgccg
    10201 cccgaaccgc ccatcgtgaa agaaggacga atcagggtag ggaagccgac ctgttcctgc
    10261 gccgccaaag cttcgttcat tgtgtggcag acaaaagatt tcgggcaaga caaaccgatt
    10321 ttttccatcg cctctttaaa gcggccgcgg tcttctgctt tgtcgatggc gtcttccgtc
    10381 gcgccgatta attcaacatt gtatttcgcc agcacgccgt tgcgcgccaa atccagcgca
    10441 cagttcagcg cagtttgacc gcccatggta ggcagaatcg catcgggccg ctctttggca
    10501 ataatttttt ccaccgtctg ccacataatc ggctcgatgt aggtaacatc cgccatttcg
    10561 gggtcggtca taatcgtggc ggggttggaa ttcaccaaaa tgactttata gccttcttca
    10621 cgcaaggctt tgcaggcctg tgcgcccgaa tagtcaaatt cgcaggcctg accgataacg
    10681 atagggccgg cgccgatgat aaggatggat tttaggtcgg tacgtttggg catgatgggt
    10741 ctttctatga aatcaaatgt ttagctaaaa atttattgct gattttgatt ggcttttaat
    10801 tgctctacga cagtattggc aatctctttt cctgatgggg ctgtaattgt gtaataaaag
    10861 ctaatactgc ctatgaagaa actgataatg gctatgatat aggctatata actttgtgtt
    10921 ttcatttttt tgtgatcatg tttgatctga tttaattctt ttaattcaga aataattgga
    10981 gttaataaat gtttttgact ttcagatcca tgcgtttcca aaagttcatt tattcctatt
    11041 agtgttggtt ttagttcgtc tgagcagcca tcaaaaggtt cttcttgttg gatttccttt
    11101 tgtaactgtc taactaaatt taaatttaat tcatcagtgt aatatagttg cttaataatt
    11161 tttctggcag caaaattatt aaggtttact gcttctgtca tttgttttat atcattaaga
    11221 tctaggttat tgtgattatt ttttaatata tcaattattc tagatttaaa tttctttttc
    11281 tttatattct ctaaatatag ctgaattgga ataaaaacaa ttataaagag caaaagaaat
    11341 atatatgtta gtgccatagc ccatgtggca attatattta tcatttatca atatcttcca
    11401 tttggtttat atattgtctc ataataagca agccgtagct cgcatgcagg gtgtgtgcgg
    11461 tacgcacgcg ttctccattt tccctgcaac cccaacccac cgtgtgcgtg ccttgcggca
    11521 cacaccctac cgatgggttc aaaggtcgtc tgaaaaaacg ctcgcggcgt ttgttccatc
    11581 tgccttgcga taaaggctgc ctgaaaaatt cccgctgttg atatttcggg cattctttct
    11641 caaaccgcct gcaaccgcaa aatccgctgc aacctgcccc ctcccccgtg ggggagggtt
    11701 ggggagaggg cattccccga atttcggcaa cctttcccaa caactcaacc accgaaatac
    11761 aagccttgtg gcttgttgcc ctctctctag ctctctccca cgggagagag gacggagtgg
    11821 ctgtagggct aagggttctg caaggaaaca ggttgtgcag gttgcttttt gcttttcaga
    11881 cgacctgaaa tcaacggtaa atccaagtcg cttttcaaac cgcctgcaac cgcaaaatct
    11941 gctgaaacct actccctccc ccgtggggga gggctgggga gagggcattc tccgaatttc
    12001 ggcaaccttt cctaacactc taaccgccaa gacacaagcc ttgcggcttg ttgccctctc
    12061 tctagctctc tcccacggga gagaggatgg ggtggctgtt ggggctaagg tttctgtaaa
    12121 ctaatcaggt tatgcaggct gctttttgtt tttcagacgg cctttataaa tgctcaattc
    12181 agtcggcttt atcctattat tttcaataat ccaatcaaaa tcagccatct gctatcgtgc
    12241 agcttgcttt tccaattctt gcaacacgag caggatttct gtcagcacat cgtttgtctg
    12301 ctgtaaaatc tcgtgattcc aaaaccgcag tacggtaaag cccaagctgt tgagatatgc
    12361 cgtccgcgcg tggtcgtatg cggcttgttc tgtgtgctgc cctccgtctg cttcgacaat
    12421 caacttgggc gttacgcaca taaaatcaac aatatagttt cccatcggct gctggcggcg
    12481 gaatttatag ccgttcagac ggcctgcccg caggtgctgc cacaattttg cttccgcctc
    12541 gctcatttct tggcgcatgg ctttggcgcg ttggcgcaag atggggttgt cggtggtcaa
    12601 gagtttctcg tgcgggttca ttttgttgtc gtgctgtttg ctgccctctc tctaactctc
    12661 tcccacgggg agagaggacg gggcggttgt tggggttaaa ggggctgtaa ggaaaacaag
    12721 tcgttcaggc cactttttac ttttcagacg acctgcaatc aacggttgta aatccaagtt
    12781 gcttttcaaa ccgcctgtaa ccgcaaaact tgctgcaacc taccccctcc cccgtggggg
    12841 agggctgggg agagggcatt ctccgaatcg cggcaatctt tccctaattc cctaactgcc
    12901 tcaatacaag cctttcggct tgttgccctc tctctaacgc tttcccacgg gagagaggac
    12961 ggtgcggctg tagtgattaa gggttttgca aggaaaacaa ggaaaacaag ttgtacaggc
    13021 tgttttttat ttttcaagcg acctgaaatc aacgcttgta aatccaagtc gcttttcaaa
    13081 ctgcctgcaa ccgcaaaatc tgctgcaacc taccccctcc cccgtggggg agggctgggg
    13141 agagggcatt ttccaagttg cggcaatctt tcccaaatcc cttagtcttc taaaatacaa
    13201 gccttacggt tgttgccaaa tgaaacttct tccataccca aaacttgcgt gtaaaacgca
    13261 atggttcggt caatgtcggc aacggtcagt accagatggt cgagtgcgct aatcttcatt
    13321 tcccgctttc tttttcaggc ggcggcagtg ccgtctgaaa gccattatgc ccgttttgcc
    13381 gctttcatat tgccaatgaa tttgtcaaac aaatagccga catcttgcgg accggggctg
    13441 gcttcggggt ggccttggaa gcagaacaca ggtttgtcgg tcagctcgat gccttgcaaa
    13501 gtgttgtcaa acaaggattt gtgggtaatg cgtgcgttag cgggcagggt gtcggcatca
    13561 acggcaaaac cgtggttttg gctggtgatg acgactttgc cgctgtccaa atcttgcaca
    13621 gggtggttcg caccgtggtg gctgaagcgc attttcaggg ttttcgcgcc gatggcgagg
    13681 ctgatgagct ggtgtcccaa gcaaatgccg aaaatcggtt tgccgctttc catcagtttt
    13741 tgtacggctt tgatggcgta ggtgcaaggc tcggggtcgc cggggccgtt ggataggaat
    13801 acgccgtcag ggttgagtgc caacacgtct tccgcgctcg tttgggcggg gacgacggtc
    13861 aggcggcagc cgcgcgaggc gagcatacgc aggatgttgg ttttcacgcc gaaatcgtag
    13921 gcgacgacgt ggtaaggctg ttcgtcaggg gtaacgaaac ccttgcccaa tgcccattcg
    13981 ccttccgtcc attcgtaagt ttccgtgcag gaaacttctt ttgccaagtc tttgccgacc
    14041 atactgccga acgcggcgat gagttcttgc gctttttcga ttgtggcatc cgcaccggtc
    14101 agaatcgcgc cgccttgcgc gcctttttcg cgcaacagcg tggtcaggcg gcgggtatcg
    14161 atgtcggcga tggcgacggt tttgttgcgt accagatagt cgtgcaggct ttcggaggcg
    14221 cggaagttgc tgtgcaagag cggcaggtcg cggataatca ggccggcggc ataaacgctg
    14281 cggctttctt catcttcggc gttggtgccg gtgttgccga tgtgtgggta ggtgagggta
    14341 acgatttgtt tgcagtagga cgggtcggtc aggatttcct gatagccagt catcgaagta
    14401 ttgaacacga cttcgccgga agtcgaacct tcgtaaccga ttgatgtgcc gtggaatacg
    14461 ctgccgtcag cgaggacgag gagggcgggg gtgctcatga tgggaatcct gtttttaata
    14521 aaattcttga caatgccgtc tgaagggact tcggcagggg gcatccggtc ggaaaaatcc
    14581 ggtcaaaaaa aaacacgccg cgcaagatag acgcgtaacg tgcttttcgg gcaagtgcct
    14641 ataccctgaa agcagcatat tttaagacga aacatacccg atttcaagcg caaacgcggc
    14701 aggcgggctg atatgttcgc gcgaaaacgg aaatccgtgg cgtaaaaccg cccgctccgc
    14761 gcccactatg cccgttacgc atttccccac gtccgtccgg cgaaactatg ggaatacccg
    14821 aatcgtcagg aatacctgaa tcgtca
//
LOCUS       DAXIHS010000050        14402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXIHS010000050 DAXIHS010000000
VERSION     DAXIHS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.050"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..195)
                     /locus_tag="ACFOF2_001319"
     CDS             complement(<1..195)
                     /locus_tag="ACFOF2_001319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107631.1"
                     /translation="MSDKVRWSYKSIADEQKSGQGDEAADSTDSTKPIHLVLQHLRES
                     FSFLFIRYIVLKHRHKPDIVR"
     gene            197..1051
                     /locus_tag="ACFOF2_001320"
     CDS             197..1051
                     /locus_tag="ACFOF2_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC7107632.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
     gene            1571..2803
                     /locus_tag="ACFOF2_001321"
     CDS             1571..2803
                     /locus_tag="ACFOF2_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC7107633.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            2842..3969
                     /gene="pheA"
                     /locus_tag="ACFOF2_001322"
     CDS             2842..3969
                     /gene="pheA"
                     /locus_tag="ACFOF2_001322"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC7107634.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            4003..4794
                     /gene="recO"
                     /locus_tag="ACFOF2_001323"
     CDS             4003..4794
                     /gene="recO"
                     /locus_tag="ACFOF2_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC7107635.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            4818..5546
                     /gene="pdxJ"
                     /locus_tag="ACFOF2_001324"
     CDS             4818..5546
                     /gene="pdxJ"
                     /locus_tag="ACFOF2_001324"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC7107636.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            5593..6372
                     /locus_tag="ACFOF2_001325"
     CDS             5593..6372
                     /locus_tag="ACFOF2_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC7107637.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLINVIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            6382..7149
                     /locus_tag="ACFOF2_001326"
     CDS             6382..7149
                     /locus_tag="ACFOF2_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC7107638.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMILFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            7349..7726
                     /gene="acpS"
                     /locus_tag="ACFOF2_001327"
     CDS             7349..7726
                     /gene="acpS"
                     /locus_tag="ACFOF2_001327"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC7107639.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            7808..8617
                     /locus_tag="ACFOF2_001328"
     CDS             7808..8617
                     /locus_tag="ACFOF2_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC7107640.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALRREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            8610..9032
                     /locus_tag="ACFOF2_001329"
     CDS             8610..9032
                     /locus_tag="ACFOF2_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107641.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            9081..10223
                     /locus_tag="ACFOF2_001330"
     CDS             9081..10223
                     /locus_tag="ACFOF2_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC7107642.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(10524..11342)
                     /locus_tag="ACFOF2_001331"
     CDS             complement(10524..11342)
                     /locus_tag="ACFOF2_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC7107643.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(11422..12672)
                     /locus_tag="ACFOF2_001332"
     CDS             complement(11422..12672)
                     /locus_tag="ACFOF2_001332"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC7107644.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(12669..13130)
                     /gene="tsaE"
                     /locus_tag="ACFOF2_001333"
     CDS             complement(12669..13130)
                     /gene="tsaE"
                     /locus_tag="ACFOF2_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC7107645.1"
                     /translation="MFDFPPISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHLGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            13325..14137
                     /gene="murI"
                     /locus_tag="ACFOF2_001334"
     CDS             13325..14137
                     /gene="murI"
                     /locus_tag="ACFOF2_001334"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC7107646.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALSTTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            complement(14184..>14402)
                     /locus_tag="ACFOF2_001335"
     CDS             complement(14184..>14402)
                     /locus_tag="ACFOF2_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107647.1"
                     /translation="KMPSEAFQTAFAAFGHLKIPSFPRRRESEYIRTETCIPSFPRKW
                     ESRTQNLKKPFYPISFRTDRSRFPLSWE"
BASE COUNT         3267 a         3664 c         4018 g         3453 t
ORIGIN      
        1 gcggactata tcaggtttgt ggcgatgttt caacacaata tagcggatga acaaaaaaga
       61 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt ttcgtactat ctgtactgtc
      121 tgcggcttcg tcgccttgtc ctgatttttg ttcatccgct atacttttgt atgaccatct
      181 gactttatca ctcactatgt tttaccaaat ccttgccctg attatctgga gcagctcgtt
      241 tattgccgcc aaatatgtct atggcggcat cgatcccgca ttgatggtcg gcgtgcgcct
      301 gctaattgcc gcgctgcctg cactgcccgc ctgccgccgt catgtcggca agattccgcg
      361 tgaggaatgg aagccgttgc tgattgtgtc gttcgtcaac tatgtgctga ccctgctgct
      421 tcagtttgtc gggttgaaat acacttccgc cgccagcgca tcggtcattg tcggactcga
      481 gccactgctg atggtgtttg tcggacactt tttcttcaac gacaaagcgc gtgcctacca
      541 ctggatatgc ggcgcggcgg catttgccgg tgtcgcgctg ctgatggcgg gcggtgcgga
      601 agagggcggc gaagtcggct ggttcggctg cctgctggtg ttgttggcgg gcgcgggctt
      661 ttgtgccgct atgcgtccga cgcaaaggct gattgcacgc atcggcgcac cggcattcac
      721 atctgtttcc attgccgccg catcgttgat gtgcctgccg ttttcgcttg ctttggcgca
      781 aagttatacc gtggactgga gcgtcgggat ggtattgtcg ctgctgtatt tgggtttggg
      841 gtgcggctgg tacgcctatt ggctgtggaa caaggggatg agccgtgttc ctgccaacgt
      901 ttcgggactg ttgatttcgc tcgaacccgt cgtcggcgtg ctgctggcgg ttttgatttt
      961 gggcgaacac ctgtcgcccg tgtccgcctt gggcgtgttt gtcgtcatcg ccgccacctt
     1021 ggttgccggc cggctgtcgc atcaaaaata aagttgggaa gcggtatttg atgattgccg
     1081 aataggctga aatctttcca tctccattcc tgcgaaagcg ggtatccgga acgaaaagac
     1141 ggatatttat ccgaaataac gaccatcttt gcatcgtcat tcccgcgcag tcgggcatcc
     1201 ggttttttga gtttcggtta tttccgacaa attgctgcag cgttggatgt ccggattccc
     1261 gcctgcgcag gaatgacggg attttatagc ggattaacaa aaatcaggac aagacgacga
     1321 agccgcagac agtacagata gtacggaacc gattcactgg gtgcttaagc accttagaga
     1381 atcgttctct ttgagctaag gcaaggcaac gctgtactgg tttttgttaa tccactatat
     1441 cgttccggtt cgtccggttt tgccggggct tttgttgccg cctgtttgtg ccggtgtgtt
     1501 aaaattttcc gtttccgcgt attgtgtttt ccgccgccgg gcggtttgtt tgcgaatcgg
     1561 acgagaattt atgccttctg cccattatcc tgaaatgagc gaaaaactga tggcggtttt
     1621 gatggcgatg ctggttacgc tgatgccgtt ttccatcgat gcctacctgc ccgcgattcc
     1681 cgaaatggcg cagtcgctga acgcggatgt ccaccgcatc gaacagagcc tgagtttgtt
     1741 tatgttcggc acggcgttcg gacaggtggt cggcggttcg gtgtccgaca tcaaagggcg
     1801 caaacccgtc gccctgaccg gtctgattgt atattgcctt gccgttgccg ccatcgtatt
     1861 tgcttcgagt gccgaacagc tcctcaacct gcgtgtcgtg caggcattcg gtgcgggcat
     1921 gactgtggtc atcgtcggcg cgatggtgcg cgattattat tccggacgca aagccgccca
     1981 gatgtttgcc cttatcggca tcattttgat ggttgtgccg ctggtcgcac ccatggtcgg
     2041 cgcattgttg cagggcttgg gcggctggca ggcgattttt gtttttctgg cggcgtattc
     2101 gctggtgctg ctcggtttgg tgcagcattt cctgcccaag cctgccgtcg gcggcaaaat
     2161 cggacgggac gtgttcgggc tggtggcggg gcggttcaag cgcgtattga aaacccgtgc
     2221 cgcgatgggt tatctgtttt ttcaggcatt cagcttcggt tcgatgttcg cctttctgac
     2281 cgaatcttcc ttcgtgtacc agcagcttta ccgtgttacg cctcatcaat acgcttgggc
     2341 gtttgcactc aacatcatca cgatgatgtt tttcaaccgc gttaccgcgt ggcggctcaa
     2401 aaccggcgtg catccgcaaa gcatcctgct atgggggatt gtcgtccaat ttgccgccaa
     2461 cctgtcccaa ctcgccgccg tgctgttttt cgggttgccc ccgttttggc tgctggttgc
     2521 gtgcgtgatg ttttccgtcg gcacgcaggg cttggtcggc gcgaacacgc aggcatgctt
     2581 tatgtcttat ttcaaagaag agggcggcag cgcgaacgcc gtgttgggcg tgttccagtc
     2641 cttaatcggc gcgggcgtgg gtatggcggc gaccttcttg cacaacggtt cggcaaccgt
     2701 gatggcggta accatgaccg cgtccacctc ttgcggcatt gcgcttctgt ggctctgctc
     2761 acatcgtgcg tggaaagaaa acgaacagag cgaatacctt taacatcaaa tgccgtctga
     2821 aaccgtttca gatggcattt gatgttagaa tgcacgataa attactgttc aggcgaaatt
     2881 atgtcccaaa ctatcgacga actcctcctt ccccaccgca acgccatcga caccatcgat
     2941 gccgaaatcc tgcgcctgct caacgaacgt gcgcaacacg cccacgccat cggcgaactg
     3001 aaaggcacgg gcgcagtata ccgtccggag cgcgaggtcg ccgtgttgcg ccgcattcag
     3061 gatttgaaca aaggcccgct gcccgacgaa tcggtagcac gcctgtttcg ggaagtgatg
     3121 agcgagtgcc tcgccgtcga acgcccgctg accatcgcct atctggggcc gcagggcacg
     3181 tttacccagc aggcggcgat taagcatttc ggacacgccg cgcacaccat ggcgtgtccg
     3241 accatagaca actgcttcaa gcaggttgaa acccgtcagg cagactatct ggtcgccccc
     3301 gtggaaaact cgaccgaagg ctcggtcggg cgcacgttgg atttgcttgc cgttaccgcg
     3361 ttgcaggcgt gcggcgaagt cgttttgcgc atccaccaca accttttgcg taaaaacaac
     3421 ggcagcaccg aaggcattgc caaagtcttt tcccacgcgc aggcgttggc gcagtgcaac
     3481 gactggttgg gcagacacct gcccaacgcc gaacggattg ccgtgtccag caatgccgaa
     3541 gccgcaaggc tggttgccga atcggacgac ggtacggttg ccgccatcgc cggacgcacg
     3601 gcggcggaaa tctacggact cgatatggtt gccgagtgca tcgaagacga accgaacaac
     3661 accacgcgct tcttggtgat gggacatcac gaaaccggtg caagcggcag cgacaagact
     3721 tcgctggccg tttccgcgcc caaccgggca ggcgcggttg cctcgctgct gcaaccgctg
     3781 actgagtcgg gtatttccat gaccaagttt gagagccgtc cgagtaaatc cgttttgtgg
     3841 gaatacctgt tcttcatcga catcgaagga caccgccggg acgcgcagat tcagacggca
     3901 ttggaacgct tgggcgaacg cgcttcgttc gtcaaagtca tcggttcgta cccgaccgcc
     3961 gttttgtagc ggcggcagcg ttcagacggc atttcaggca tgatgtccga acaccgagtc
     4021 aaccacgaac ccgtcttcct gttggcttcc tcgccctggc gcgaaagcag cctgcgggtc
     4081 gaagcgttca gccgccgtta cgggcgtgtg gcacttttgg caagaagcgc gcgcaaacgg
     4141 cagagcgaat tgcgcggcgt gttggtgccg tttgtgccgg tgagtgcgtc ttggtatggt
     4201 tcgcaagagt tgaagaccct acatcgcgcc gaatggatag gcgggtggcc gcagccgcag
     4261 ggtagggcgt tgttcagcgg attgtatgtg aacgagttga tgctgaagtt gaccgtccgc
     4321 gaagatccgt tgcccgagtt atacgacgcg ttggcggaag tgatggaggc ggtgtgctgc
     4381 aaagccgctt atatcgacga cttgcgccgt ttcgagtggc ggctgctgaa cctgttgggc
     4441 gttgcccccg atttgaaccg cgacggggac ggcgggacga ttgcggcagg cggcacatac
     4501 cttgtccgcc cggaaacagc cgtcttcccc gtcggaaaag gatttgccgt accgccgcac
     4561 gccgccggcg ttgtcgcccc cgggcagagc ctgatcgatt tgcgcgaagg cagtttccgc
     4621 actgccgaaa gcctgcaaca ggcattgaaa atcacacggc tttttatccg ccacctgttg
     4681 cccgaggggc tgaaatcgcg gcaggtgttg gaacagatac ggcagtttga ccgcaaagaa
     4741 accgcccggg aaaccgtccc gacttcggac ggcacggctt caaatgccgt ctgaaggcag
     4801 aaataaaagg aaagattatg cttttaggtg tcaacatcga ccacatcgct accgtccgca
     4861 atgcgcgcgg cacgacttat cccagccccg tggaggcggc actggttgcc gaaacgcacg
     4921 gtgccgacct gattaccatg cacctgcgcg aagaccgccg ccacatcaaa gacgcggacg
     4981 tgtttgctgt caaaaacgcc atccgcacgc gcctgaacct tgaaatggcg ttgacggaag
     5041 aaatgctcga aaacgcgctt aaagtgatgc cggaagacgt gtgcatcgtg ccggaaaaac
     5101 gtcaggaaat cacgaccgaa ggcggcttgg acgtattggc gcaacaagac aaaattgccg
     5161 aattcaccaa aatcctgacc gacgcgggca tacgcgtgtc cttgtttatc gatgccgacg
     5221 acaggcaaat ccaagccgcc cgtgatgtcg gcgcgcccgt tgtcgagctg cacacaggcg
     5281 cgtatgccga cgcgcgcagc cacgccgaac aaatcaggca gttcgagcgc atccaaaacg
     5341 gcgcgcattt tgccagcgat ttgggcttgg tcgtcaacgc cggacacgga ctgaccatac
     5401 acaacgttac ccccatcgcc caaatcctcg ccatccgcga actgaacatc gggcattcgc
     5461 tgatttcgca ggcacttttc ctcggattgc ccgaggccgt gcgccaaatg aaggaggcga
     5521 tgttcagggc aaggctgctg ccgtaagggc aggcaaaccg tttcagacgg catttcacga
     5581 caggagtatg ttatgaatca aaagtatatt ttatctgcaa acaataatag tttgatagaa
     5641 gaaattcaca atacagtaca gcgtattggg tattgtattg ttcgcggtct taatctaaac
     5701 catcttgatg acagccggag aaacaagaaa ttatttgact ttctatctca attaggaatg
     5761 ctgacaaacc acaaagacga tggttttaaa tctatatttt gggatattaa atatcgcggc
     5821 gatgactatg taataaataa tgatataact ttctcggaag atgttggaga atgtccactt
     5881 catagtgatt catcttttag tgaaaacccg gaaagttatt tggttatgta tgtagtaaaa
     5941 tcagccaatg atggaggtaa ttccctattt ttaagttcat cagatattgt caatcagtta
     6001 tctaaaacag aaaccggtaa aaaacactta aaaacattaa cgggcaattt atatccattt
     6061 aaaacaccag catcatttga taaaaaacaa ggtgtgagat ggggtaatat cttatcggtc
     6121 aatactcaaa tgattagatt tagaagtgat tgtatctata aaggtattga agaaaataga
     6181 aataaagtat caaaggaaat ggtacttgca cttgattatc ttataaatgt tataaaaaat
     6241 gcgagtgata ttcaagaatt ttctgcacaa gatgatggtt tgattattat tgacaatgtc
     6301 aatggcttgc atgccagaac tgattatacg gataaaaaca ggcattatat tagagcaaga
     6361 attactgtat aaaggacggt tatgcaagaa ataatgcaat ctatcgtttt tgttgctgcc
     6421 gcaatactgc acggaattac aggcatggga tttccgatgc tcggtacaac cgcattggct
     6481 tttatcatgc cattgtctaa ggttgttgcc ttggtggcat taccaagcct gttaatgagc
     6541 ttgttggttc tatgcagcaa taacaaaaag ggtttttggc aagagattgt ttattattta
     6601 aaaacctata aattgcttgc tatcggcagc gtcgttggca gcattttggg ggtgaagttg
     6661 cttttgatac ttccagtgtc gtggctgctt ttactgatgg caatcattac attgtattat
     6721 tctgttaatg gtattttaaa tgtatgtgca aaagcaaaaa atattcaagt agttgccaat
     6781 aataagaata tgattctttt tgggtttttg gcaggcatca tcggcggttc aaccaatgcc
     6841 atgtctccca tattgttaat atttttgctt agcgaaacag agaataaaaa tcgtatcgca
     6901 aaatcaagca atctatgcta tcttttggcg aaaattgttc aaatatatat gctaagagac
     6961 cagtattggt tattaaataa gagtgaatac ggtttaatat ttttactgtc cgtattgtct
     7021 gttattggat tgtatgttgg aattcggtta aggactaaga ttagcccaaa tttttttaaa
     7081 atgttaattt ttattgtttt attggtattg gctctgaaaa tcgggcattc gggtttaatc
     7141 aaactttaat tcattattaa atgccttaac tccttattaa ataattggca cgatgtttta
     7201 gaatttcaaa tgcaaaaggt tacaataaaa attgttaccg acaaaacccc gaaagtggat
     7261 attcacgcca ttttaacgcc ccaagaaatt gacggcattc atcatcacat tcatcactac
     7321 ccgcaaccaa gggcgaagga gcgcaaatat gatttacggc atcggtacag acatcgtttc
     7381 cctcaagcgc atcatccgct taaacaaaaa attcggacag gcgtttgccg tgcgtatcct
     7441 cactccggaa gagctgcttg aatttcctca ggcgggcaaa cccgtcaact accttgccaa
     7501 acgctttgcc gccaaagaag cctttgccaa agccgtcggc acgggcatac gcggcgcggt
     7561 ttccttccgc aacatcggca tcgggcatga cgcattgggc aagcccgaat ttttctacgg
     7621 ccccgccctg tccaaatggc tggaggaaca aggcatcagc cgcgtcagcc tcagcatgag
     7681 cgacgaagaa gacaccgtat tggcgtttgt cgttgccgaa aaataatgcc gtctgaaatg
     7741 cggcaaaccc gttgacggca ttgcccgtcc ctcatttgca ctccgaccga ccaaccgcgt
     7801 acccgccatg attcaagaca cccgacccct tatccgcgtc gttgccggca tcctgctcaa
     7861 ccgagacggc gactacctgc tcagttcccg ccccgaaggc aaaccctatg ccggatattg
     7921 ggaatttgcc ggcggcaagg tcgaagcggg cgaaaccgac ttccaagccc tgcggcgcga
     7981 gtttgaagaa gaactcggca tccgcatcct cgccgccacg ccttggctga ccaaaatcca
     8041 ttcctacgaa cacgcccgcg tctgcctgaa attcctatgg gtcaaccccg gccaatggac
     8101 gggcgaaccg caatcccgcg aagggcagga atggtcttgg cagaaggcgg gtgattttac
     8161 cgttgccccc atgctgcccg ccaacggcgc gcttttgcgt tcgctgtccg ttccgcgccg
     8221 tttgtacggc agcctgaaaa cgggtttgca cggggagaac agtatgggcg cgtaccgcgt
     8281 cctgcctttg ggttcggcag agggaagcgg tgcgaacgtt ttgatggagg cggcgcaatg
     8341 gcaggacaga cccgaacacg ccgacagcgt gtggatggtg gtgcagaccc gcgaacaatg
     8401 gcggcaggcg caggaaaagg gcgcggatgc ggtcgtttgg cgcgtgtgcg atgatgttca
     8461 ggcacaagag gcggcagaag ccctgcggca gggcgtatcc gtgccgctcg tacttgcagc
     8521 aaacggacag acggttgcac gttatggaaa actatggctc ggattggggg cgcacgtggt
     8581 ggtaagggat gaaacaatag ggaagaatca tgaataaaaa ccgtaaatta ctgcttgccg
     8641 cactgctgct gattgccttt gccgccgtca agctcgtttt gttgcaatgg tggcaggcgc
     8701 agcagccgca agctgtggcg gcgcaatgcg atttgaccga gggttgcacg ctgccggacg
     8761 gaagccgcgt ccgcgccgcc gccgtttcaa ccaaaaaacc gtttgatatt tatatcgaac
     8821 acgcgcccgc cggcacggaa caggtcagca tcagcttcag tatgaaaaat atggatatgg
     8881 gtttcaaccg ctatatgttc gagcggcaac cgtcggggac ttggcaggca gtacgcatcc
     8941 gcctgcccat ctgtgtcgaa ggcaggcgcg attttacggc ggacattaca atcggcagtc
     9001 ggacatttca gacggcattt accgccgaat aaacctttca atccgccatt gccggaacat
     9061 ccgtccggaa aggacacgtt atgaatactt tatatacact tttcgccacc tgcccgcgcg
     9121 gcttggagac cgttttatct caagaactcg aaagcctcgg ctgtaccgat gtacaagtgt
     9181 ttgacggcgg cgtttcctgc cggggcggat tggaacaggt ttacgccgcc aacctgcatt
     9241 cgcgtactgc cagccgtatc ctgctgcgcc tgaccaaagg gacataccgc aatgagcgcg
     9301 acatctacaa actcgccaaa aatatcaact ggtttaattg gtttacttta cagcagacgt
     9361 tcaaagtcaa agtcgaggca aagcgtgcca acgttaagag catccaattt gtcggactga
     9421 ccgtcaaaga tgccgtctgc gacgctttcc gcgacattta cgacgcacgt ccgagcgtgg
     9481 acaaagccgc gcccgatgtt cgcatccacg cctttttgga cgaacgcaat gtcgaaatct
     9541 tcatcgacac ttcgggcgaa gccctgttca aacgcggcta ccgtctcgat accggcgaag
     9601 ccccgctgcg cgaaaacctt gccgccggac tgctgctctc ggcaggctac gacggcacgc
     9661 agccgtttca agacccgttt tgcggcagcg gcacgattgc tatcgaagcc gcttggattg
     9721 ccacaggccg cgcaccgggc atgatgcgcc gcttcggttt tgaaaaactg caaaatttcg
     9781 ataaaacgct gtgggcggat ttgcgccgcc gcgccgaagc gcaaacccgc cccgtccgcg
     9841 ccccgattgc aggcagcgac agcgaccgcc gcatcgttca gacggcattg gacaacgcac
     9901 gcagtgccga agtggacgac atcgtttcct tcggcgttgc cgacgcgcag tccgtccgac
     9961 cgaacggcga aaacggcatt atggtgtcca atccgcccta cggcgtgcgc cttgaggaag
    10021 tccacgcttt gcaggcgctt tatccgcagt tgggtacgtg gttgaaaaaa cactatgccg
    10081 gctggcgggt cggcatgttt accggcgaca gggaaatgcc caaattcatg cgcctgtcgc
    10141 caaaacgcaa aattccgctt tataacggca acctcgattg ccgcctgttc ctgattgata
    10201 tggtgcaggg atcgaaccgt tgaggaaagt gtacaaaaat gccgtctgaa aaatgttcag
    10261 acggcttttt ttaatatccg gccgatatta tagtggatta acaaaaatca ggacaaggcg
    10321 acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt gagcacctta
    10381 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaattcact
    10441 ataaccgaag gtaagggagg ggtgtgtcca ctgtgccgcc gcatttgccg cgtgcggaag
    10501 atacggataa caaccccttg gaattaaagg gagaagttgc gggaaatatc catatcaggc
    10561 tgtgggccgt acttatttac ggtattttcg tatgccgaaa taaaatattt cttgaataaa
    10621 ccgtaatcgc cggtttcgta ataggctacg acagcctcgg cataggcggg ataactgtcg
    10681 ggggagaata cgcaaggaaa aaaaccggag cgcatcagcg acaaggtcat gcagtttctg
    10741 gcagtacgtt tattgcaatc tttaaaatat tgaagatacg ccaaattatt atggagataa
    10801 acggcgcggt cgaaaggatt ttctattttg ggagcttctt gaagcaacca ttttaattcc
    10861 gtatccaaac tttgcgggtt agataaaggg gtgtagtctg tcccgcttat ggtaacgctg
    10921 tcgcggcgga ctacgccgcc ggagcctttt tccagtaagt tttcggaaat caggctgtgg
    10981 gtggttttta agaaatccaa ccagtcaaac ggctcggggc tgtccaaacc ggacagcagg
    11041 tgccggtaac tttccctcag gttgattaac attacggcat ccgaatataa cttcccgcct
    11101 gcggtttgcc cgagtttaag caatgcctgc gtatcgtatt gattataagt gtttccttcc
    11161 aatttggcag aactgtacac aaaatcgata ccgatttctt caatattacg cacggagtcc
    11221 gttaaaacag ataaaggaag ccgatcggac aattcggtca ggtattcttg ttcgccggct
    11281 gtaaaaatgg ggcggatatt taaaaaatcc tgttggtatc tcaatttatc catacctttc
    11341 atcataaaaa cgcggtttgg ggcggaagta ggtttatgcc tgccttcatc cgacaactat
    11401 attgaaacac cgccttttca atcaaccccg cttcaatacg gatgtattga tgtagcgttg
    11461 gacacccgag gcaatggatt gggcgcactg ccgacggaag gattcgctgc ccagcagctt
    11521 ctcttcggca ggattggaca ggaaggcggt ttcgaccagg atagacggca tatcgggcgc
    11581 gcgcaaaacg gcgaaattgg cttcgtccac cctgcctttg tgcagatggt tgagcctgcc
    11641 caattcttca agcaccagtt tgccgagttt gcggctgtcg cgcagcgtgg cggtttgggt
    11701 catatcgagc agggcggtat cgacattgcg gttgccgctg gtcggtacgc cgccgaccgc
    11761 gtcggcatta ttttgggttt gttccaagaa tttggcggcg gagctggtcg cgcctttggt
    11821 gttcagcata taaacccctg tgccgcgcgc ggaggggctg gtgaaagcgt cggcgtggat
    11881 ggagacgaat acgtccgccc gccgtgctcg ccctttggcg acacgcacgc ccaatgggat
    11941 gaacacgtct tcgttgcgcg tcataaagac gttgtaaccc aaggcttcca attgtttttt
    12001 ggtttcgcgg gcgatggaga ggacgacgtg tttttcctgt aggccgcccg ggctgacggc
    12061 gccggggtct tcgccgccgt gtccggggtc gatcataatg acgggtctgc gcccgttttt
    12121 gccgcgttcg gtttgtgcgg gagtgttttg agcgaggtcg gcttcagggg agccgcgacg
    12181 ggttttgttc aggctgccgt taagcagcgc catcatcgga tcgtcgggat ccatcccttg
    12241 cggatagaga tcgacgacga ggcggttttt aaagccgccg acggggggga gggaaaaaac
    12301 ttgcgcgtag gaaggctgtt tcaaatcgat gacgaggcgg acggtggtcg gtgtgttctg
    12361 acccgcgcgt atgctgcgga taaaagggtc gtccgccata accttctgag acagtccgtg
    12421 caatacggta ttgatgttcg cgttttgtat gtcgacgacc agcctgcccg ggttgtcgag
    12481 cgtgaagtgc tggtatttga gcgcggcggt gctttccagc gtcaggcggg tgtaggtgtg
    12541 cgacggccat atccgtgcgg cggtgaattg cggggcgcgt accgttttgg caacggcgga
    12601 tgcgatgggg cttagggcga acagtgtgcc ggcggtgcgg cggatgattt gtcttcgtgt
    12661 cagtttggtc atagcggcag gctttcgcgt cctcgttcgg tatgggcggt cagcaggcat
    12721 tttctgccgc cgccgccgtg tgtcaatgtt gcggtgatgt cggcgggcgg cgtaaattcc
    12781 ccgccctgtt gcggccattc gatcaggcag acgctgtttg cggcaaacag ttcgtcaagc
    12841 cccgcgtctt cccattcttc ggggaacgag aagcggtaga ggtcgaaatg gtgcaaggtg
    12901 aagcgttcca gcggataaga ttcgacgatg gcgtaggtcg ggcttttgac tgcgcccaga
    12961 tgacccaatc cgcgtaggat gccgcgtgtc agcgtggttt tgcccgcacc caaatcccct
    13021 tcgagataaa tgaccagcgg tgcgtttaaa cgggaagacc acgccgcgcc caaatcgagt
    13081 gtggcggctt cgtcggcaag gaatcgggaa atggggggga aatcaaacat aggggcttta
    13141 aaacttttat gtggcggatc ggagagggcg gccggcaaac cgtgcgcccg gcgggccgga
    13201 tgcggcattg ccgtacatta taacagccaa atccgcatcc tgctttcaga cggcaatggc
    13261 tgtcaagaaa aagcggcgcg tgtacaatac gcggattgta tgtttgggac ggattggaaa
    13321 aaggatggaa aatatcggca ggcagcgacc catcggcgtt tttgactcgg gaatcggcgg
    13381 tttgaccaat gtgcgcgcgc tgatggagcg gctgccgatg gagaacatca tttatttcgg
    13441 cgacacggcg cgcgtgcctt acgggacgaa atctaaggcg accatcgaaa atttctcgat
    13501 gcagattgtc gattttttat tggaacacga tgttaaggcg atggttatcg cgtgcaacac
    13561 gattgcggcg gttgcggggc agaaaatccg tcaaaagacg ggcaatatgc ctgtgttgga
    13621 cgtgatttcc gccggcgcga aagccgcgct gtcaaccacg cgcaacaaca aaatcggcat
    13681 tatcgccacc aataccacag tcaacagcaa tgcttatgcg cgcgccatcc acagggacaa
    13741 ccccgacacg ctcgtccgca cacaggccgc gccgctgctc gtccctttgg tggaagaggg
    13801 ctggttggaa cacgaagtta cccgcctgac cgtatgcgaa tacctcaaac cattgcttgc
    13861 agacggcatc gatacgctgg tgttgggctg cacgcacttt cccttgctca agcccttaat
    13921 cggcagggag gcgggcaatg tcgcgttggt tgattctgca attacaacgg ccgaagaaac
    13981 cgcacgcgtc cttgctcagg aaggattgct caataccgac aacaacaatc ccgactaccg
    14041 tttttacgtc agcgatattc ctttgaaatt cagaaccatc ggcgagcgtt ttctgggcag
    14101 gacgatggag cagattgaaa tggtgtcttt gggttaaaac gatgacggaa agctgcccga
    14161 gattacagaa acctaaaatc ccgtcattcc cacgaaagtg ggaatctaga cctgtcggtg
    14221 cggaaactta tcgggtaaaa cggtttcttg agattttgcg tcctggattc ccactttcgt
    14281 gggaatgacg ggatgcaggt ttctgtgcgg atgtattcgg attccctcct gcgcgggaat
    14341 gatgggattt ttaaatgtcc gaatgccgca aatgccgtct gaaaggcttc agacggcatt
    14401 tt
//
LOCUS       DAXIHS010000051        13886 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXIHS010000051 DAXIHS010000000
VERSION     DAXIHS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.051"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="ACFOF2_001336"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="ACFOF2_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC7107648.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1155..1304)
                     /locus_tag="ACFOF2_001337"
                     /pseudo
     CDS             complement(1155..1304)
                     /locus_tag="ACFOF2_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1530..2249)
                     /gene="pyrH"
                     /locus_tag="ACFOF2_001338"
     CDS             complement(1530..2249)
                     /gene="pyrH"
                     /locus_tag="ACFOF2_001338"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC7107649.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2493..3347)
                     /gene="tsf"
                     /locus_tag="ACFOF2_001339"
     CDS             complement(2493..3347)
                     /gene="tsf"
                     /locus_tag="ACFOF2_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC7107650.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(3476..4204)
                     /gene="rpsB"
                     /locus_tag="ACFOF2_001340"
     CDS             complement(3476..4204)
                     /gene="rpsB"
                     /locus_tag="ACFOF2_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC7107651.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4377..4610)
                     /locus_tag="ACFOF2_001341"
     CDS             complement(4377..4610)
                     /locus_tag="ACFOF2_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107652.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            4688..5269
                     /locus_tag="ACFOF2_001342"
     CDS             4688..5269
                     /locus_tag="ACFOF2_001342"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC7107653.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            5330..5920
                     /locus_tag="ACFOF2_001343"
     CDS             5330..5920
                     /locus_tag="ACFOF2_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC7107654.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            6457..7923
                     /locus_tag="ACFOF2_001344"
     CDS             6457..7923
                     /locus_tag="ACFOF2_001344"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC7107655.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(8110..8484)
                     /locus_tag="ACFOF2_001345"
     CDS             complement(8110..8484)
                     /locus_tag="ACFOF2_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC7107656.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(8722..9021)
                     /locus_tag="ACFOF2_001346"
     CDS             complement(8722..9021)
                     /locus_tag="ACFOF2_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC7107657.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(9176..9955)
                     /gene="map"
                     /locus_tag="ACFOF2_001347"
     CDS             complement(9176..9955)
                     /gene="map"
                     /locus_tag="ACFOF2_001347"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC7107658.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(9995..10663)
                     /locus_tag="ACFOF2_001348"
     CDS             complement(9995..10663)
                     /locus_tag="ACFOF2_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107659.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKEKETPKENHTKPDTPKNTPPKPHKEIL
                     DNLF"
     gene            complement(10671..11279)
                     /locus_tag="ACFOF2_001349"
     CDS             complement(10671..11279)
                     /locus_tag="ACFOF2_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC7107660.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(11343..11936)
                     /locus_tag="ACFOF2_001350"
     CDS             complement(11343..11936)
                     /locus_tag="ACFOF2_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC7107661.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(11941..12288)
                     /locus_tag="ACFOF2_001351"
     CDS             complement(11941..12288)
                     /locus_tag="ACFOF2_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC7107662.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            12334..13209
                     /gene="rsmI"
                     /locus_tag="ACFOF2_001352"
     CDS             12334..13209
                     /gene="rsmI"
                     /locus_tag="ACFOF2_001352"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC7107663.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     VLSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(13244..13648)
                     /locus_tag="ACFOF2_001353"
     CDS             complement(13244..13648)
                     /locus_tag="ACFOF2_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC7107664.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
BASE COUNT         2906 a         3278 c         4034 g         3668 t
ORIGIN      
        1 ctctacggat ggggcagagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc taccgtctga
      481 ttgggtgcga gagcgtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaacaaa ttataaatta aataaaatat
      961 ttctttactg gttttgtggt ttggttttca ataggctgcc cgcacatagt gggatttacg
     1021 gttgcaatgg ctgcggcgta ttttatagtg gattaaattt taatcaggac aaggcgacga
     1081 agccgcagac agtacaaata gtacggcaag gcgaggcaac gctgtactgg tttaaattta
     1141 atccactata ggcttttttc ggtcagttgc cggttaatca gtcccggcag ttcggacagg
     1201 gtgttgtctt gcgccaacct gttgcggtag cggcataagg tgctgtaatc ggggatgctt
     1261 ggtttgtcaa aacggcaaaa caggttgaaa tcggtgcggg tgatgggtac ggcgttgccc
     1321 ttatgccgat tttgtccgcc tgctgcaagg cggtttgtct aatgaaagga aaaatgccgt
     1381 ctgaacattg ttcagacggc attttcaaat ggaaggcaat atagtcggca tatgaaaaat
     1441 tgatgtataa ggcaggatgg gtttaggctt gttaatttcc atataataaa tggccgtatg
     1501 aagcccatat gcgactatat ctgccggaat cagcagtgaa ccagtgtacc ctcgttttcg
     1561 ccggttacca cgcgtttgag cgagccttct ttagcgatac cgaatactac gatattgagc
     1621 ttgcgttcgc ggcagagggc gaaagcggtg gcatccatca ctttaaggtt tttcaacaag
     1681 gcttcgtcaa aagtaatggt ttcgtagcgt gttgcagatg ggtctttttt cgggtctgcg
     1741 gtgtacacac cgtcaacgtt ggtggctttg agcatcacgt cgcagttcat ttctgcaccg
     1801 cgcaatgcgg cggcagtgtc ggtcgtgaag aacggattac cggtaccggc ggcgaaaatc
     1861 acgactttgc cttcttccaa atattgaatg gctttaggac gggcgtaagt ttcggcgatt
     1921 tgctgcatag acagtgcgga ttgtacgcgc gctttgatgc ccaaagtttc aaatgcgtct
     1981 ttgagtgcca acgcgttcat tacggttgcc atcatgccca tgtagtcggc ggtggcgcgg
     2041 tccatactgc ctgcttgtgc agatacgcca cggaaaatgt taccgccgcc gacaacgata
     2101 ccgacctgca cgcccatttt aacgacttcg gcaatttcgc cgacagtttg aacgatggta
     2161 tcgtgattga tgccgaacgg atcggaaccc atcagggatt cgccggagag tttcagtaat
     2221 acgcgtttgt atttgatttg ctgtgtcatg ggatattctg ctttctcgtt ggggatgttg
     2281 ccggcttccg ggcgggatgc cgtctgaaag gtgtatgcct tggcagggcg tatgcggcgt
     2341 gccggcggtg tgaaaacggg ctgctgaatt tgccgccgct gttttcgggc ggggcgggca
     2401 tttgaaccct gccgccggaa aatatgggcc tttcccaaaa aaaagcaccc tgattcgttt
     2461 ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggcagc ggctacttct
     2521 gccgcgtaat cgacggcttt tttctcgata ccgtcgccta ctttgtagcg gacgaagctg
     2581 accacttcag tgccgttttc tttagcgaat tgggcaacag tttggtcggg gttcatcacg
     2641 aacgcttggc cgttcagagt gatttcagcc aagaatttgc ggatacggcc ttcaaccatt
     2701 ttagcggcga tgtcggcagg tttgccggaa gcgatagctt gttcagtgta gatgtggcgt
     2761 tctttttcaa cggtttcagc atctacttcg gcttcactta cgcattgtgg tttagcggca
     2821 acgatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc aaccaatacg
     2881 ccttcggtag ccaaagcacc gtggatgtag gcaaccagtt ggttggcagt gtcgatcact
     2941 tggaagcggc gaacagacat gttctcgccc aatttagcga tgatggcttt acgttctgct
     3001 tcaaccagtt cgctcagttc ttcaacggta gccggttttt tctcggcagc ggttttagca
     3061 acgaagttgg caaattctac gaagcccgcg tctttggcaa cgaagtcggt ttcgcagttt
     3121 acttcgacca atgcgccgac attgccgttg atggcgtaag ctaatacgcc ttcggcagcg
     3181 gtacgaccgg ccagtttacc ggctttcgcg ccggatttga tgcgcaggat ttcttcggct
     3241 ttgtcgaagt tgccttcagc ttcaaccaag gcttttttgc attccatcat gcccaagccg
     3301 gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt gaatctccta
     3361 gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc gcgaacggac
     3421 gttcagacgg catatttgag aaaaggggca taacgcccct cttctgtaca ccgaattact
     3481 cggcagcagc ttcttgggca gcggctacag tttcttgcag cgcttggttt ttgccttcca
     3541 aaactgcgtc ggcgatgccg cggcagtaca ggcggatggc tttggcggag tcgtcgttgc
     3601 cggggataac gtatttcacg ccgtcagggc tgttgttggt atcgactacg gcgataacgg
     3661 ggatgcccaa tttctcagct tcgaccagag tacctttttg gtagccggta tcgataacga
     3721 aaatcgcgtc aggcaggcct ttcatgtttt tgataccgcc caaagaacgt tccagttttt
     3781 caacgtcgcg ttgcatttcc agaatttctt ttttgttgaa accgccttca gcagcgtttt
     3841 ccagggcagc agttttttct tccaggcgtt tgatggattg cttaacggtt ttgtagttgg
     3901 tcagcatacc acccaaccag cggtaatcaa cgaaaggcat accggcacgg gtagcttctt
     3961 cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta cctttgttgg
     4021 caaccagacg acgtacggct tcttgcgcct cttggaacat cggcagggtt ttttccaagt
     4081 ttacgatatg gattttgttg cgcgcaccga aaatgtattg tgccattttc gggttccaaa
     4141 aacgggtttg gtggccgaag tgaacaccgg cttcaatcat ctgacgcata gtaatttgag
     4201 acattttatt tccttgaaag ggttaagagc acacattcaa tcgcatagaa cttgataggt
     4261 cacgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa aatagtgtgt
     4321 ttccgaaatg gaaaactgac ggattatagc ggataagatg ccgtctgaaa agggattcag
     4381 ggttttgtgc cgctgtcttt ttgccgttgt gcgcgctgct gttcggattc cgcctgttcc
     4441 tgctgcctca tcaggtggtt gcggcggcgg acggtaatgg tgaaaaccgt gaacacggtg
     4501 ggcagcaccg cccaaaaaac caaataaatc aaggcgcgcg caatactcgg ctgcgcggcg
     4561 gaatacataa cggcaacaaa aagatagccg atggcgacga tgtgccacat tgtgtgcgtc
     4621 ctgtttgaat gttaacaaaa tcgttataat cgcaatattc taccgcaatc ctgaaaggcc
     4681 gtctgaaatg aggaacgagg aaaaacgcgc cctgcgccgc gaattgcgcg ggcggcgttc
     4741 gcaaatgggg cgggacgtgc gggcggcggc aacggtaaaa atcaaccacc tgctcaaacg
     4801 ttatattaaa aaagggcgga aaatcggcgt gtattggccg atgggcaagg aattgcgttt
     4861 ggacggcttt gtccgcgcgg cgcaaaaacg cggtgtaaaa ctttatctgc cttatatcga
     4921 accgcgttcg cggcggatgt ggtttacgcc gtatcctgaa agcggaatgg aacgggagcg
     4981 catacggggc agggcgaagt tgaacgtgcc gcagtttgca gggcgcaaaa tccgcgtgca
     5041 cggtttgtcg gtattgctcg tcccgcttgt cggcatagac cgcgaaggct accgcttggg
     5101 gcaggcaggc ggctattacg atgcgacgct tgcagcgatg aaataccgtt tgcaggcaaa
     5161 aaccgtgggc gtgggctttg cctgccaact ggtcggcacg ctgccgcgcg aggcgcacga
     5221 cctgccgctg gacggttttg tgtcggaggc ggggatattg tgtttttagc cgttcagacg
     5281 gcatttcggg cggaaatgcc gtctgaaggc ggaatattgg gagaaaagga tgtcggaaaa
     5341 gataatactg ccggtcttgt cattgggcgg cgttcggctc gaaccgttgg atgtgcatca
     5401 tgaaacgggt ttgcgcgagg cggtttgcga tggggaggtt tggaagctgg gggtaacgtc
     5461 cgcgccccat ccggataggg tggcagacta tatcgggaca gcattggcaa cgcgtttggc
     5521 gtttgcggtt gtcgatgaag aggcgggcag ggtggtcggg acaacggcgt attatcattt
     5581 tgaaccgcag attccgcgtt tggatatcgg attcacatgg tatgcggcat cggcacggcg
     5641 gacacggata aacacctgtt gcaaaatcat gctgttggat tacgcatttg atgttttggc
     5701 ttgccgttgc gtgggatggc gcacggatat tctgaacctt gcctcgcaac gtgccatcga
     5761 acggctgggt gcggaaaaag acggcgtgct gcgtatgcat atgctccgta aagacggcag
     5821 tgtgcgcgat acggtcgtgt acagtatgct gagggaagat tggtgcaaaa acagggaaat
     5881 cctgaccggc aggttggcag ggtatggcgt gcaggtgtga ggcagtattt tcaaatttat
     5941 agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc
     6001 taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt
     6061 cgccttgtcc tgatttttgt taatccacta taaaaaactt aacgcaatat gctgacaatc
     6121 ttttaaaaat taatatataa acaattttga ttttttatct attaaaaatt tgatatatat
     6181 agataaataa ctatttttat ttttcatttc gcagtttccc gacttggcgg gcgacagaaa
     6241 aactttgcat ttcccagtaa aatccactac attcacgata acatattaca agcccctgaa
     6301 gcggtgtcgg aagccatgct tgcaacgcat tccaaccccg aaacagcata ctcggacaca
     6361 caccattgcc ggttttccct gcttttgcga ttcaggcgtt tgtagtacgg caaataacca
     6421 taagggcaga agatgcctgt tttttaggag taaaccatgg ctgaagcgac agacgttgtc
     6481 ttggtgggcg gcggcattat gagcgcgact ttgggcgttt tgctcaaaga actcgaaccg
     6541 tcttgggaaa tcaccctgat tgaacgcttg gaagatgtgg cgttggaatc gtcaaacgcg
     6601 tggaacaacg ccggcacggg gcattccgcg ctgtgcgaat tgaactatgc gccgttgggt
     6661 gcaaatggga ttatcgatcc ggcgcgcgcc ctcaatattg ccgaacagtt tcatgtcagc
     6721 cgccagtttt gggcgacgct ggtcgcggaa ggcaagttgg aagacaattc cttcatcaat
     6781 gccgtgccgc atatgtcttt ggtgatgaat gaagaccatt gttcttatct tcaaaaacgt
     6841 tatgacgcgt ttaaaaccca aaaacttttt gaaaatatgg aattttccac cgatcggaac
     6901 aaaatttccg attgggctcc gctgatgatg cgcggccggg acgaaaacca acccgtcgcc
     6961 gccaactact ccgccgaagg cacggatgtc gatttcggac ggctgacgcg ccaaatggtg
     7021 aaatatttgc agggcaaggg cgtaaaaacc gagttcaacc gccacgtcga agacatcaaa
     7081 cgcgaatccg acggcgcgtg ggtgctcaaa accgccgata cccgcaaccc cgacgggcag
     7141 ctcaccctcc gtacccgctt cctcttcctc ggcgcgggcg gcggcgcgct gaccctgctg
     7201 caaaaatccg gcatccccga aggcaaaggc tacggcggct tccccgtgtc cggcctgttc
     7261 ttccgcaaca gcaaccccga aaccgccgaa caacacaacg ccaaagtgta cgggcaggct
     7321 tccgtcggcg cgccgccgat gtccgtcccg cacctcgaca cacgcaacgt ggacggcaaa
     7381 cgccacctta tgttcggccc ttacgcaggc ttccgttcca acttcctcaa gcaaggctcg
     7441 cttatggatt tgccgctgtc catccatatg gacaatctct atcctatgct gcgcgccggc
     7501 tgggcgaata tgccgctgac caaatacctg ctgggcgaat tgcgtaaaac caaagaagaa
     7561 cgcttcgcct ccctgctgga atactacccc gaggcaaacc ccgacgactg ggaactcatc
     7621 accgcagggc aacgcgtcca aatcattaaa aaagactccg aaaaaggcgg cgtgctccag
     7681 tttggtacgg agattgtcgc ccacgccgac ggctcactcg ccgcattgct gggcgcgtcg
     7741 ccgggcgcat cgaccgctgt gccgctgatg atccggctga tgcaccaatg cttccccgag
     7801 cgcgccccgt cttgggaagg tcgtctgaaa gagctggtac cgggttacgg catcaagttg
     7861 aacgaaaacc ctgaaagggc ggatgaaatt atcgcctata ccgcgaaagt gttggatatt
     7921 taagccgaat ctttcaagaa aagccgtacc cccatatggt gttgatgccg tccctgccgg
     7981 ttatgccctt catccggcag ggcaggtttt ggtacacggg gcaggtaatc gttttttccc
     8041 ttttgtgaat aatggaacag tcaaaaaaca gcgttttcat ttctgaaaac gctgtttttt
     8101 gttgcctgat taacgtgggg aacagtcttt gaagacgatt tggttgtcag gtgcggtaat
     8161 cataatgggc tgtttgcggt aggatttacc atccatcacg ccggtaccca aaacataacc
     8221 gccttctttg ccgtagaatg tttccacatt gtcggatttg tccaaattga caggcatttg
     8281 cacgcgtttg ccgttgatga cggcggaagc gtatgtggtc aggccctgtt tgttgaagcc
     8341 gtaggttact ttgacttttt taccttgctg gcagacgtag ctgacggttt ttttggcaac
     8401 ggtggggttg ttcgtgccgg cagcagcagc catactgctg agcgcgattg cggaagacag
     8461 gattgcggtg gtcagaagtt tcatttttat tcctttggat ggatgtggag tttgcccgtt
     8521 tcgagctgtt tgacattcta atgtcggcgt atcctgaaat aaacaggaaa ttggataaag
     8581 tttgtaaaaa ggcgggttcg ggcggttcgg cggtttgagg caggggcggc aggcgggata
     8641 cggcggcctg tccggtgttt cagacggcat tgtttgcgtt tttcggaaat gaaaaacctt
     8701 cgggcatccg gaggttgcgg tttaactttg tttttttctt tcggacttgg tctgtttgga
     8761 caattcggca tacaggtcgg gatggtgttt tttcaacagg gcggcgattt ccatatcttt
     8821 tttgttgact ttcgccaaat cgatgcattc gatatggacg aggcggagca gttcgcgtac
     8881 gggtttgggc attttgcggc cggtttcgta gcgcgagcct ccagactggg taacgccgac
     8941 cttgccccag aagtctatct ggttcagccc ggtcttttta cggatggctt taacatcttc
     9001 tatattttca aatattttca taaaacttcc tttaagtttg tctggttgcg gggcaaattc
     9061 cgatttgctt tgttttcgta atgtgccggg tcaagcctgt ttttatatta tatatcatat
     9121 ctgccgtttc tttcagacgg cattgtttgt ttttatgggg cggatacgtc cggtttcagg
     9181 gtttgccgga ggcggggctg acggtgagga tttcgtagcc ggtttcggtc accaagactt
     9241 cgtgttccca ttgggcggag agggagcggt ctttggtaac caccgtccag ccgtcgttga
     9301 ggatacgcag gtggcgtttg ccttggttga tcatcggttc gacggtaaaa atcatacccg
     9361 gttttagaac ggggccctgt cctttttttc cgtagtgcaa cacttgcggg gcttcgtgga
     9421 aaccgcgccc gatgccgtgt ccgcagaatt cctgtacgac ggaatagccg gcgttttcgg
     9481 caacctgttg gcaggcgtaa cctacgtcgc ccagtgtcgc gccgggtttg acggcttcta
     9541 tgcccgccat catggaggcg tgggttacgt cgatcaggcg ttgggcgatg ggggagactt
     9601 tgccgacggt aaacatacgg ctggagtcgc cgtggaagcc gtcttttttg atggtgaggt
     9661 cgatgttgat aatgtcgcct tctttgagcg gcttgtcgtc gggaatgccg tggcagatga
     9721 cgtggttgac ggaggtgcag caggatttgg ggtagggcgg gttgccgtag tgcagggggg
     9781 cgggatagcc gccttggacg ttgacgtggt agtcgtaaac gagtttgtcg atttcgtcgg
     9841 tggttacgcc gggtttgacg aattgtccga tgtagtcgag ggcttcggcg acgagtttgc
     9901 ccagctcgcg cattttttcg atttcttcgg gggttttgat gatgatgccg ttcataatgc
     9961 ttccttggat tgcgggtgtg cctgccgtgc cgggtcagaa gaggttgtcg agaatttctt
    10021 tatggggttt gggcggcgtg tttttcgggg tgtccggttt ggtatggttt tctttgggcg
    10081 tttctttttc tttgggcgtt tctttttctt tgggtgtttc tttgggctgc gcctgttttt
    10141 cgggtgtttc ttttcggggt tcgggacggg ggacgtttgt gggcagtacg ggggtttctt
    10201 tgagtgtgtt ttcagcttgt gttccggcgt cgtccggctg cctgtcggtt tgagctgtgt
    10261 cggcaggttg cggtttgacc cggtttttct tgggtgcggc aggggacggc agtccgctgc
    10321 cgctttcgtc tgtgccgtcc ggctgtgcgg gttcggatga ggcggcgtgg tggtgttcgg
    10381 gttgggcggc atcttgtccg actacgccgt ttggcagcca gaattcggtt tcgcgggggc
    10441 tgtcggtgtg ttgcggttcg gtttggaggg ttttgccgcc gttcgggttg aatgtgctga
    10501 cgatgcctat tgccaatgcg ccgatggcgg tgatacagat gagcaatacg acacgtatca
    10561 ggagtttggg ggtcagcctg aagggtttgt tcggtttttt tgccattttg attgtgcttt
    10621 tggggtgtcg ggcaatgccg tctgaaggcg gttcagacgg cattgccgag tcagcgttgg
    10681 acgtagtttt ggtagagggt gatgactttt tgtacgccga cggtggtgct gactttttgg
    10741 gtaatctgcg cctgttcttc gggggtgagg atgcccataa cgtaggttac gttgccgtag
    10801 gtaacgattt tgacgcgcgc ctgtgtggcg gggctgatgc ccaacagcgt ggcgcggact
    10861 ttggatgtgt tccaagtgtc gccggcgatg tcgccggcag tgcgcggcag ggaggcgacg
    10921 gtaatgtagt tgtatacgcc ttcggcggcc tgttcggaac gtgcaatctg accgacgaac
    10981 tgtttctcgc cttcggtggc gacttgtccg agcagcagca ggtggcggtt gtagccgaca
    11041 acggagattt ggggcgtgta gcctttggtt tggttgtttt ggcgcagata ggagcgggcg
    11101 gtggtttcga tacgcaacgc cataacgttg tcgtcggttt gcgcgccggt ggttcggcgg
    11161 tcgacggcgg atttcgcgcc gacggcggcg cttccgatta ctgcgctgac gcagccgcta
    11221 agggcaaggc tgaaaatggc ggcaatcagg gtgcggacgg tgtgcggttt gggtttcatc
    11281 gggtgcttcc tttcttgggc gtttcagacg gcattgcttt gcgccatgcc gtctgaaaag
    11341 ggttacattc cttccagcag tacggagtcg atacagtcgc acatggcgtg tatcagcagg
    11401 atgtggtttt cttgaatacg ggcggtgcgc ggatggggaa cgttgagcaa aacgtcggtg
    11461 tctttgagta tggcggcgat tttgccgccg tcgcggccgg tcaaggcgat gacgtgcata
    11521 tcgcgttcgt gtgcggcttt gacggcttcg atgacgttgg cggaattgcc ggaggtggaa
    11581 atgccgacca atacatcgcc tgcacgtccg agcgcgcgca cctgtttgct gaatacgtgg
    11641 tcgaaaccgt agtcgttgcc gatggctgtc agcgcggaag tgtctgttgt cagcgcgaca
    11701 gcggcgagtt ccatgcgttc tttttcaaaa cggccggtca tttcggcggc gaagtgttgc
    11761 gcgtcggcag ccgaaccgcc gttgccgcag gccaggattt tgccgtcatt catcaggcat
    11821 tgcagcatca gctcggcagc ctgtacggtc ggctcgacca atacttttcc ggcttcctgc
    11881 ttggcacgga tgctttcggc aaaatgggcg gcaacgcgtt cttgtaatgt cgtcatatcg
    11941 tcaacctgta atattctgta tccactcggg cgggcggctg ccttcgatga gtaccgcgtc
    12001 gaggcggcac ggtacgtttg tcaacctgtt ctgttgcaga taatactcta cacttcgttg
    12061 cagtttcaat aatttggatg gggaaatgct gtatgcggca ccgccgaatt gccgattttt
    12121 gcggtatttt acttcaacaa acagaatcat gccgccgttt ttgacaatca ggtcgatttc
    12181 gccgtaggcg cagtgccagt tgcgggcaag cagtgtgcag ccttgggatt ggaggaaggc
    12241 aagcgcggca tcttcccctg cctcgccctg tttgtggttt aggcgcataa tcggtttaac
    12301 ctttataatg tttgtttttc agacggcatt cttatgtttc agaaacattt gcagaaagcc
    12361 tccgacagcg tcgtcggagg gacattatac gtggttgcca cgcccatcgg caatttggcg
    12421 gacattaccc tgcgcgcttt ggcggtattg caaaaggcgg acatcatctg tgccgaagat
    12481 acgcgcgtta ccgcgcagct tttgagcgcg tacggcattc agggccaact cgtcagcgtg
    12541 cgcgaacaca acgaacggca gatggcggac aagattgtcg gctatctttc agacggcatg
    12601 gttgtggcac aggtttccga tgcgggtacg ccggccgtgt gcgacccggg cgcgaaactc
    12661 gcccgccgcg tgcgtgaggt cgggtttaaa gttgtccctg ttgtcggcgc aagcgcggtg
    12721 atggcggctt tgagtgtggc tggtgtggcg ggatccgatt tttatttcaa cggttttgta
    12781 ccgccgaaat cgggcgaacg taggaaattg tttgccaaat gggtgcgggc ggcatttcct
    12841 atcgtcatgt ttgaaacgcc acaccgaatc ggggcaacgc ttgccgatat ggtggaattg
    12901 ttccccgaac gccgattaat gctggcgcgc gaaattacga aaacgtttga aacgttctta
    12961 agcggcacgg ttggggaaat tcagacggta ttgtctgccg acggcaacca atcgcgcggc
    13021 gagatggtgt tggtgcttta tccggcgcag gatgaaaaac acgaaggctt gtccgagtcc
    13081 gcgcaaaaca tcatgaaaat cctcacagcc gagctgccga ccaaacaggc ggcggagctt
    13141 gccgccaaaa tcacgggcga gggaaaaaaa gctttgtacg atctggcact gtcttggaaa
    13201 aacaaatgat gccggacgga ccggcaccat agtgcgaacg gaatcaacgg acacgccccg
    13261 tcatgcggta cagcacgtct ttgccttgaa cacggtggac gacgaccatg gcgacgtgtc
    13321 cgacgacggc ggcaaacagc agccagccca aattgccgtg gaacgtgttg cccaagtttg
    13381 ccatccactc gattttttcc ggcgaaccct gcatcacttc aacgccgaac actttcaacg
    13441 ggccgcggcc gctgccgtat tggcggatca tgccgataac gggaacggca agcatgagca
    13501 gatacagaat gccgtgtcct gccgccgcag ccttgctgtc gctttgcgga cgcttggcgc
    13561 ggttggcaac cgcccacacg atgcgcaatg taatcaccgt cagcgtaagg aaaccgaaag
    13621 atttgtgcag gccgaacagg ctgcccaccc attccgcctc ttcgtatagt ggattaacaa
    13681 aaaccagtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc
    13741 cttgtcctga tttttgttaa tccactataa atcgtccaca ggacaatggt ggtcggaatg
    13801 ccgatgaaac cggcaacggt cagccagtgc agcgtacgtg tcaacgggtc gtaaacggca
    13861 ggttggtttg tgttcataaa aaactt
//
LOCUS       DAXIHS010000052        13862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXIHS010000052 DAXIHS010000000
VERSION     DAXIHS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.052"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            1..103
                     /locus_tag="ACFOF2_001354"
                     /pseudo
     CDS             1..103
                     /locus_tag="ACFOF2_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(470..892)
                     /locus_tag="ACFOF2_001355"
     CDS             complement(470..892)
                     /locus_tag="ACFOF2_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC7107665.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEE
                     RYKLSRSEFEQLWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
     gene            complement(1035..2681)
                     /gene="pgi"
                     /locus_tag="ACFOF2_001356"
     CDS             complement(1035..2681)
                     /gene="pgi"
                     /locus_tag="ACFOF2_001356"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC7107666.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            complement(2802..3650)
                     /gene="hexR"
                     /locus_tag="ACFOF2_001357"
     CDS             complement(2802..3650)
                     /gene="hexR"
                     /locus_tag="ACFOF2_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC7107667.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3700..4686)
                     /locus_tag="ACFOF2_001358"
     CDS             complement(3700..4686)
                     /locus_tag="ACFOF2_001358"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC7107668.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4667..5362)
                     /gene="pgl"
                     /locus_tag="ACFOF2_001359"
     CDS             complement(4667..5362)
                     /gene="pgl"
                     /locus_tag="ACFOF2_001359"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC7107669.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5642..7087)
                     /gene="zwf"
                     /locus_tag="ACFOF2_001360"
     CDS             complement(5642..7087)
                     /gene="zwf"
                     /locus_tag="ACFOF2_001360"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC7107670.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7603..9438
                     /gene="edd"
                     /locus_tag="ACFOF2_001361"
     CDS             7603..9438
                     /gene="edd"
                     /locus_tag="ACFOF2_001361"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC7107671.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9619..10257
                     /locus_tag="ACFOF2_001362"
     CDS             9619..10257
                     /locus_tag="ACFOF2_001362"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC7107672.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10272..10490
                     /locus_tag="ACFOF2_001363"
     CDS             10272..10490
                     /locus_tag="ACFOF2_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107673.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(10514..11459)
                     /locus_tag="ACFOF2_001364"
                     /pseudo
     CDS             complement(10514..11459)
                     /locus_tag="ACFOF2_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11622..12662
                     /gene="mutY"
                     /locus_tag="ACFOF2_001365"
     CDS             11622..12662
                     /gene="mutY"
                     /locus_tag="ACFOF2_001365"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC7107674.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            13079..13639
                     /gene="sodC"
                     /locus_tag="ACFOF2_001366"
     CDS             13079..13639
                     /gene="sodC"
                     /locus_tag="ACFOF2_001366"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC7107675.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3244 a         3762 c         3498 g         3358 t
ORIGIN      
        1 aggatttgac cgctacctgt tccgtcccta tgcccgcagc ctgaaagggc aaatagtgaa
       61 agcgcagata agtggaaaaa gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
      121 ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
      181 cgttctcttt gagctaaggc gagccaacgc tgtatcggtt taaatttaat tcactataaa
      241 aacgacaaaa acgcaaaagc cgccgacatt cccgcatcca agtttcagtc aatcagataa
      301 ccttggattt ctttggtttt cgcattgatt tctctggtac ggcagtcaga ttgtgccacg
      361 ccgtattcgt cgccgtcggc gcatttggca ttcaaaccgt tttgtcagtt gcggtactcg
      421 ggcatgacgg tttcggcaat atcggcgggc agtgcctgaa ccgcgctgtt cagagccgct
      481 ttggcagttc tttcttgtcc gcttccctga ccatttcgtc gataagggct tttttacggt
      541 tgtacagctc ttccaagcgt gcttcggttt ccaccgtttt gcatgccagt tcgctgactt
      601 ttatgccgtt tggcgtttca ccttcggctt tcgcattgtt ggcacagatt ttgtccatac
      661 cgcttttcca tgttttctgt gaggcttgca gcttattctg tacggtttga ggcaatcctt
      721 tccagagctg ctcgaactcg ctgcgcgaca gcttgtagcg ttcttcccat tcggatacgc
      781 ggtcgacttc ctgctcccga cccaatgcct cctgcgccgc cgcttcttct gccgcttcaa
      841 gttcttcgtt cctttgtttc gctttgtcca acggctcttt aatcagtgcc atagctgcgg
      901 aacatatatg tttaaattta tgcaaaccat catatcggga ttgcacacgc tctgcaagtt
      961 taccgacggt tttcctgttc gataaaaatg ccgtttgaaa cggtcggcgt tcagacggca
     1021 tttttccgca ggttttattt gcggttggtc tgcaggtaga ggttgatcag gcgttcggtc
     1081 gagctgtcgt gcttttgcga cccggtttcg ccggtcagtt cgcccaaaat ggttttagcc
     1141 agttgtttgc cgagttccac gccccactgg tcgaagctgt tgatgcccca aatgatgcct
     1201 tgtacgaagg ttttgtgttc gtacatggca atcaggctgc ccatattacg cgggttgacc
     1261 ttgtccatca ggatgaggtt ggtcgggcgg ttaccggaga aggttttgtg cgggaccagc
     1321 tcttcgatgc gcgcctcatc cataccctgc gctttgagtt cggcgcggac ttcgtcgggg
     1381 gttttgccgc gcataaaggc ttctgcttgg gcgaagacgt tggcaagcag gatttcgtgg
     1441 tgtccgggca ggttgctgcg tttttcaagc gaggcaatca ggtcgatggg ggtaatgtgc
     1501 gtgccttggt gcagcagttg gaagaaggcg tgctggccgt taatgcccgt ttcgccccag
     1561 ataatcggcg aggtttcgtg tccgactgct ttgccgtcca acgtaacctg tttgccgtta
     1621 ctttccatat cgagctgctg gatgaatttg ggcaggcggt gcaaatgttg gtcgtaaggc
     1681 gcgatgacgt ggctgccgcc gccgtagtag ttgatatacc agatgccgat gagggcgaga
     1741 atgacgggca ggttgcgctc gagcggtgtg ttgatgaagt gttggtccat caggtgcgcg
     1801 ccgttgagca tttcaatgaa gttttcttcg ccgagataca gcataatcgg caatccgatg
     1861 gcggaccaca ggctgtaccg accgccgacc caatcccaaa attcaaacat attggcggtg
     1921 tcgatgccga attcggcgac ggctttttga ttggtggaaa cggcggcgaa gtgtttggca
     1981 acggcttctt cgtcgcccgc atgattcaaa aaccattcgc gcgcggtcag cgcgttggtc
     2041 agcgtttcct gcgtggtaaa tgttttggag gcgatgatga acagcgtggt ttcggggtgg
     2101 actttggaca atacgtcgcg cagttgggag ccgtccacgt tggagacgaa gtgcatattg
     2161 aggcgcggat gaccgaaagg tttgagcgcg gtacacatca tcagcgggcc caaatccgat
     2221 ccgccgatgc cgatgttgac aacgtcggta atgacttggt tggtatagcc cagccagctt
     2281 ccgctgcgga cttcgtgtgc aaattcgccc atacgttgca aaacgcggtt gactttgggc
     2341 atcacatctt caccgtcaac cacaatcggc gaattggtgc ggttgcgaag ggcgacatgc
     2401 aggacggcgc ggttttcggt ggtattgatt ttttcgccgt ggaacatctg ccgcatccgc
     2461 tccggcacgc ctgcttctcg ggcaagctcg aacaaaagcg acatggtttc gtcgttgatg
     2521 cggtttttgg agtagtccag cgtcagtccg ccgacttgca gccagtagcg ttccgcacgc
     2581 tgcgggtctt gctcgaacat ttcgcgcata tgcaatgttt tgctgtcgtc aaagtggttc
     2641 cacaatttcg accatgcggg taagtcgtga aagtgtttca tctatatgct cctgaatgag
     2701 gttttttgtt gtgggatgaa aaggctgctg gaaactgccg caagccgccg acggccgttg
     2761 ttcggcattt cagacggcat ttgtgggatg ccgtctgaag gtcaatcttt gtcgtaatcg
     2821 atgtgcttgt tgtgtatgct ttttttgctt ttctgcaatt gcaggctggc agcatcgccc
     2881 aagcgcaggg caagtccgat ggcgagaatg tcgatgacgg caagctgcaa gaggcgggaa
     2941 accatgggcg tgtagagttc ggcattttcc tgtgtggcaa cgctcaacac gcagtcggca
     3001 agttgcgcca gaggcgaatc gttgcgggtc agtgcgatga cggacgcgcc gttttctttg
     3061 gcgatgctga ccgcatccaa aagttcgata gacgaacccg tgttggaaat ggcaaccaaa
     3121 acatcctgat cgctcaaaac ggatgctgcc atcagctgcg tgtgcgtatc gacataggcg
     3181 accgtggaca ttccgaaacg gaaaaacttg tgttgcgcgt cctgcgcaac gataccggaa
     3241 ttgccgacac cgtaaaactc gacgcggcgg gcgtgcatca gcgtggcaat ggcgttttcc
     3301 agctccgact ctttcaggaa gcggcgttcg cccaacagcg aggcggcggc attgcccaac
     3361 actttctcga ccacgcttgc catatcgtcg tcggcgttga gttcttcatg gacatagggc
     3421 ataccctcat gaccgatgct ggcggacaac gcgagcttga actcgggcag ccctttataa
     3481 cccaagctgc ggcagaatcg gatgacggtc ggctggctga cggacgcacg ttcggcaatt
     3541 tcggcaacgg cggcatggac gaaccatttg ggttccgcca atgcacattc ggcgactttg
     3601 cgttccgcac cggaaaggtt tgccagtgat tcgctgattt tgcttaacat aatgatatgc
     3661 ccttcgataa tgcagccccg ctgcaaggag ccgctgtggt taaacgtttc tcaaatggtt
     3721 gtcgagagcc gcagccgcac cagaaattcc gggaaactcg ctcaagacga catacacggg
     3781 aatcgcggca agatatgctt caaacctgcc cttgttctcg aaacggctgc ggaacgggga
     3841 agttttgaaa tattccaaca cgcggggaat aatgccgcca cacaggtaca cgccgccgcg
     3901 cgcgcccagc gtcagggcga ggttggaagc aaccgtgccg agcatggcgc agaagatgtc
     3961 caaagtctga cggcacaaag gcgacgcgcc gctcaaagcc ttttccgtga tttcagacgg
     4021 catcagtttg gcgggtttgg ctttctgttt tgcagccaaa gcctcgtaaa ccaagctcaa
     4081 gcccgcgccg ctcaaaaagc gttcggcgga aacatggccg tatttgtttt tggcgtactg
     4141 ccaaatcagc acttccatat cgtcaaacgg cgggaaactg gtatgcccgc cctcgcccgc
     4201 caaagccacc cagcctgcgt ggctgtgcac caatccgctc acgcccaggc cggtaccggg
     4261 gccgataacg gctttggggg caaattcgac aggcttttgc ccgcctacct gcatcaggtc
     4321 tttgcttgaa gtctgcgtta ccgccaatgc ctgcgcggta aagtcgttca aaaggatgag
     4381 ggtgtccagc cccaaagtct gacgggtggt ttcgatggaa aacgcccaat ggtggttggt
     4441 catctgcacc cagtcgccca aaatcgggtt ggcgatggca aatgccgcgt gccgtacggc
     4501 tgttgcaccg ctttgattca gataggcacg caccgcatcg gtaaccgtat cgtagtcttt
     4561 acacggaagc acggcggctt tttcaatgac gcgcggcgcg gtttccagcg caaagcgtgc
     4621 attcgtcccg ccgatatcgg cgaccagtcg gggatatccg gcttgtttat tcggcgtaga
     4681 agacatggca gttcactcct tgatggttca aaacgaggtt gatcggatat tcgcggtttt
     4741 cgccttgtgc ggcttggtcg aacacggctt ttttctcttc gccccgtatc gccaaaaaca
     4801 catgccccgt atgggcaatc gcatccaagg tcatactgac gcgctcgtgc ggcgcggtaa
     4861 cgggcgtggt atgcaccagc gcgacacctg ccgaaccgtc gattgccgtc tgaaactgcg
     4921 gagctttcgg gaaaatcgaa gccgtatgcc cgtcgtttcc catacccaaa accaaaacat
     4981 cgggctgttt gtaatgtttc agtgcataat cgacaacagc atcgggatgt aattcggttt
     5041 cagtttttcc gtcttccacc ataggaatcc acattgccgc ttccgctttg ttcttcaaca
     5101 ggtattcgcg caccaaaccg gtattgctgt cggcgtggac ggtcggcacg atgcgttcat
     5161 ctgccaaggt gatgccgacg tttttccaat ccaaatcttt ttgcgacagg gcgttgaaaa
     5221 atgcaatcgg cgaacgtccg ccggaaactg ccaacaccgc accgcccttc tcgtccagtg
     5281 cgccctgcaa agcatccgcc actgcgtcag ccaaagactg cgccgcttct gccgcatttt
     5341 cgtattcgtg ccaaacaaac atatttgtgt ccttttttta tttcagacgg catattccgt
     5401 tatggaaacg ggttgagcaa tatgccggtc gaacagttgt ttatgctttt gataccaaat
     5461 atcgggactg ctttttatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc
     5521 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
     5581 actgctttac ttgcgttcag acggcatttc aaaccccatg ccgtctgaac gcattattgt
     5641 attactgctc ttcgtgccac ttgtgtccgt cgcgcgccaa tagttcgcgc gcggcttcag
     5701 gcccccacga gtgtgcgccg tagccgtgcg gcggcgtggt gttatttgcc cagttttcca
     5761 aaatcggcat cacatattcc cacgcggctt cgagttcgtc gcggcggttg aacaaagcga
     5821 gtttgccgtt aatcacatcc agcagcaggc gctcgtaagc ttccgcgcgg cggccttcca
     5881 atgctttgcc caaatcggtt gccagcggca cggtttcgac cttatttcct gcccccgggg
     5941 ttttcatctg cgtatagagg cgcacggatt catatggttg caactcgata acgagccggt
     6001 tgggcgcggt gcgactgcct tcaaaaatat ggctgttcaa atctttgaag ttcaaaacga
     6061 tttccgccac tttgcccgcc atgcgcttgc cggtacgcag gtagaaggga acgcccttcc
     6121 agcgttcgtt ttcgatttcg gctttaatgg cgacgtaggt ttcggtaaag ctgtcttgcg
     6181 gaacgttgat ttcttcaaga tagccgttca tgcctttggc ggcggtatat tgtccgcgca
     6241 cgacattttc attgacagat tcgacggtca gcggcttcaa tgacttgatg actttgactt
     6301 tttcatcgcg caccgcgtcg gcatccaagc tggcgggggc ttccatcgca gtcatgcaca
     6361 gcatctgcat caaatggttt tgcaccatat cgcgcaacgc gccggtaatg tcgtaaaact
     6421 cgccgcgctc ttccacaccg agctgttcgg cgatggtcaa ctgcacgctt tcgatgtatt
     6481 tattgttcca cagcggctcg aacattacat tggcaaaacg cagcgcaagc aggttttgca
     6541 ggctttcttt gccaaggtag tggtcgatgc ggtaaatttg cccttctttg aaataacgcg
     6601 caacatcggt attgatttgc tgggaagaag ccaaatccgt acccaacggt ttttccaaaa
     6661 ccacacgcac gttgtcggcg ttcaaaccga ttttggcaag gttttcacat gtttgcgcga
     6721 agaatttcgg cgcggtggaa agatagatga cgacattgtc ggtttctttg cgcgctttga
     6781 ccaaatcgcc caaagcggca aaatcgtccg gctgcgtaac atcgactttg agatatgcga
     6841 gacgttcgac aaacgatgcc caagcctcat cggaaaaatt ttctttcaca tggattttgg
     6901 aactggtttc caccttcgcc agaaaacctt cggtatccaa ctcgctgcgg ctgaccccta
     6961 aaatacgccc ttcgggatga agcagaccgg caacatgcgc ctggtacaga cagggcaaca
     7021 gcttgcgcat cgccaaatcg ccggtcgcac cgaacaacac caaatcaaaa tttgtttgtg
     7081 tactcatcgt attatctcgt caggaaagaa tttttcgatg ccgtctgaaa cctgtttccc
     7141 ccatcacgct gcatcgcaat atcggaaaca aaggcaggcg gcataatgag tagtaatact
     7201 acacaccgct acactttttg tctattccca tttttacaat ttatttgacc tagtccaaaa
     7261 atcgggcagg tttcccctat tccgttacaa caatcggaag attctgcgat ttaaatcaaa
     7321 tttcttttcg atgtctgatt tttttgtaac aaaattacaa attttgtact ataatagcac
     7381 ccgcttccca ctttcagacg gcataccttt taaaatatag tggattaaca aaaatcagga
     7441 caaggcgacg aagccgcaga cagtacagat aatacggcaa ggcgaggcaa cgccgtactg
     7501 gtttttgtta atccactata ctcaccgtct gaatacccga tacaaaaatc agaaacgcac
     7561 aaacaaatcc ccaatacccc ccgttccgac aggagaccga ccgtgaacac tactcctatc
     7621 cactccaaac tcgccgaaat caccgggcgc attattgaac gcagccgtcc gacgcgtgaa
     7681 aaatatctgg cgaagatccg cagtgccaaa caaatgggac gcttagagcg caaccagctc
     7741 ggttgctcca acttggcgca cggctatgct gccatgccta aaagtatcaa aatcgaaatg
     7801 cttcaggaaa ccgtccccaa cttaggcatc atcaccgcct acaacgacat ggtttccgca
     7861 caccagccgt ttaaagactt ccctgaccaa atcaaagacg aagcgcagaa aaacggcgcg
     7921 accgcccaag tcgccggcgg cacacccgcc atgtgcgacg gcatcacgca aggctacgcc
     7981 ggtatggaac tgtcgctgtt ctcccgcgac gtgattgcca tgagtaccgc cgtcgggctg
     8041 tcgcatcaaa tgtttgacgg cagcctgttt atgggcgtat gcgacaaaat cgttccgggt
     8101 ttgatgatag gcgcgctttc gttcggtcat attccgggta tcttcgtccc cgcaggcccg
     8161 atgtccagcg gtatcggcaa caaagaaaaa gcccgcaccc gccagctttt cgccgaaggc
     8221 aaggtcggac gcaacgaact tttgaaaagc gaaatgggtt cttaccacag cccgggcacc
     8281 tgcactttct acggcacggc aaactccaac caaatgatga tggaaatgat gggcgtgcac
     8341 ctgcctgccg ccgccttcgt ccacccttac accgacctgc gcgaagccct aacccgctac
     8401 gccgccggac acctcgcgcg cggcatcaaa aacggcacga ttaaaccttt gggcgaaatg
     8461 ttgaccgaaa aatcctttat caacgccttg attggcctga tggcaaccgg cggttcgacc
     8521 aaccacacca tgcacctcgt tgccatggca cgcgccgccg gcgtgatttt gaactgggac
     8581 gacttcgacg aaatttcctc catcatcccg ctgctcattc gcgtttatcc gaacggcaag
     8641 gccgacgtga accactttac cgcagcgggc ggactgcctt tcgttatccg cgaattgctg
     8701 aatgcaggcc tgttgcacga cgatgtcgat accgtcgtcg gacacggtat gcgccactac
     8761 accaaagagc ctttcctcat cgacggcaaa ctcgaatggc gcgaagcccc cgaaaccagc
     8821 ggcaacgacg acatcctgcg taaagctgac aacccgttct cccccgacgg cggtctgcgc
     8881 ctgatgaaag gcaacatcgg acgtggcgtg attaaagtgt ccgccgtgcg cgaaggctgc
     8941 cgcattattg aagcgcctgc catcgtgttc aacgaccaac gcgaagtgtt ggctgcgttt
     9001 gaacgcggcg agttggaacg cgattttgtg tgcgtcgtcc gctaccaagg cccgcgtgcc
     9061 aacggtatgc ccgaattgca caaactgacc ccgcctttag gcatcctgca agaccgcggc
     9121 ttcaaagtgg cgctgctgac cgacggccgt atgtccggcg cgtccggcaa agttccagcc
     9181 tccatccaca tgacacccga agccctgatg ggcggcaaca tcgccaaaat ccgtaccggc
     9241 gacctgatcc gcttcgactc cgttaccggc gaactcaacg tcctgattaa cgaagccgaa
     9301 tggaacgccc gagaagtcga aagcatcgac ttgggcgcga accaacaagg ctgcggccgc
     9361 gaacttttcg ccaacttccg cagcatgacc agcagcgccg aaaccggcgc gatgagcttc
     9421 ggcggcgaat ttgcctgatg cgcgtttcag acggcctttt cagaccgaag gccgtctgaa
     9481 aaattattca agtgttttaa gatagacgta ggtcggattc tcgaatccga cacagccgtc
     9541 caagatgtcg gtttcttgaa tccgacctac aacctgtccc atcttaataa aataccccat
     9601 cccacccgga gaaccgaaat gtccaaactg accccccgcg aaatcctgac cgccggcgca
     9661 gttgtaccgg taatggcgat tgacgacttg agtactgccg tcgatttgtc ccacgccctt
     9721 gtcgaaggcg gcatccccac cctcgaaatc accctgcgca cccctgtcgg cctcgatgcc
     9781 atccgcctga ttgccaaaga agtgcccaac gccatcgtcg gcgcgggtac ggttaccaac
     9841 cctgaacagc tcaaagccgt cgaagacgca ggcgcggttt tcgccatcag cccgggtttg
     9901 cacgaatccc tcgccaaagc cggccacaac agcggcatcc ccctgattcc cggtgttgcc
     9961 accccgggcg aaatccaact ggctttggaa cacggcatcg acaccctcaa actcttccct
    10021 gccgaagtcg ttggcggcaa agccatgctc aaagccctgt acggccctta cgccgatgtt
    10081 cgcttctgcc cgacaggcgg catcagcctc gccaccgcgc ccgattactt ggcactgccc
    10141 aacgtcctgt gcgtcggcgg ctcatggctg acgccgaaag aagccgtgaa aaacaaagac
    10201 tgggacacca tcacccgtct cgccaaagaa gcggcggcat tgaaacccaa agcctgaacc
    10261 gcatcgtaaa aatgccgtct gaagcccttt ccagtctcag acggcatttt gccgcttggc
    10321 ggcgcagtcg gcatacacgg cagcaccact ccgacatact gcccctaaaa tgcccatccg
    10381 ccttccgcat aataaaaata acgttcagtt cattcgacag cagccggaca gcccatacta
    10441 cgcggctgaa aaaatgccgt ctgaaacgca ttcagacggc atccgcttaa aaaaacaact
    10501 gattcaaggc cgattaatcc gcttccaaaa ccactttcat cgcttgattt tcggcggcgt
    10561 gtttgaacac gtcgtaggct ttttccaatt cgctgaattt gaaacggtgg gtcaacattt
    10621 tggtgtaatc gacggagctg ctggaaattg ccttcatcag catttcggtg gtattggcgt
    10681 ttaccaggcc ggtagtgatg gcaagctttt taatccacag tttttccagt ttgaaatcaa
    10741 cggattgacc gtgtacgccg acaacggcga tatggccgcc gggtttcaca atgtcttggc
    10801 acatattcca agttgcaggg ataccgacgg cttcgatcgc gcagtccacg ccgtcttcgc
    10861 cgacgatagc gacaacttgc ttgcttacat cgccggaagc agggttaatg gtatgggttg
    10921 cgcccaactc tttcgccagt ttcaaacggt tttcgtccat atcgcaaaca atgatggcgg
    10981 cggggctgta cagttgggcg gtcggcaagg cgtcgctcaa caacagggcg acttcttcgt
    11041 tgacgttgtc gggcagcggc acgaggctgt tgtcggcata aggcgtgcgg acgtattctg
    11101 cctgcgtgcc gtcaatcatg tagcccaaaa tccagccgcc gttgcggcaa tgcgaataaa
    11161 gttggatttt gcagttgtcg caagtacagc atttgctgac gcatgaaata atgactttat
    11221 cgccgacttt gatgtttttt acagcctcac cgacttcttc tacaataccg atgccttcat
    11281 gaccgagaat acggccgtcg gcgacttcgg gtttttttgc ctttccaaat acccaaatcg
    11341 gtaccgcaaa tcgtggtttt gacgattttc accaccgcat cggtcggatc gataatctgc
    11401 ggacggggtt tttcttcaaa acggatgtcg tttgcgccgt gataaaccat tgctttcatg
    11461 ctgatactcc ttgcttgttg ataaataatt tcaataccgc aataaagttt ccttatatga
    11521 gttatatgcc cctacaaaaa ataagtcaat aagaattatt ttcacaatgt tatacaataa
    11581 catactattt taaatataaa taaaaccacc gattgatatt aatgaacaca cccatcccct
    11641 tctccgaacg gctcatccgc tggcaaaaac aacacggtcg ccaccacctc ccttggcagg
    11701 tcaaaaaccc ttattgcgtc tggctttccg aaatcatgct ccagcaaacg caagtcgcca
    11761 ccgtgttgga ctactatccg cgcttcttag aaaaattccc gaccgttcag acgcttgccg
    11821 ccgcgccgca agacgaagtg ttgtcgttgt gggcgggctt gggctattac agccgcgcgc
    11881 gcaacctgca caaagccgcg caacaagtcg tcaggcaatt cggcggcacg tttccgtcgg
    11941 agcgcaaaga cttggaaacc ctctgcggcg taggcagaag caccgccgcc gccatttctg
    12001 ccttctcctt caaccgccgc gaaaccattt tggacggcaa cgtcaaacgc gtactctgcc
    12061 gcgtgttcgc ccgcgacggc aatccgcagg acaaaaaatt tgaaaactcg ctctggacac
    12121 ttgccgaaag cctgctgccg tctgaaaacg ccgatatgcc tgcctataca caaggtttga
    12181 tggatttggg cgcgaccgtg tgcaaacgga cgaaaccctt gtgccaccaa tgcccgatgg
    12241 cggacatctg cgaagcaaaa aagcaaaacc gcaccgccga gctgccgcgc aaaaaaaccg
    12301 ccgccgaagt accgaccctg ccgctttact ggctgattgt ccgcaaccgg gacggcgcga
    12361 ttttgctgga aaaacgcccc gccaaaggca tttggggcgg gctgtattgc gtgccgtgtt
    12421 ttgaaagttt gaacgggctt tccgactttg ccgccaaatt ctccctgacc atggcagata
    12481 tggacgaaca aaccgccctg acccaccgtc tgacgcaccg gctgctgatg attacgccgt
    12541 ttgaaggaca aatgccgtct gaaagccctt cagacggcat ttggataaag ccggcgcatt
    12601 tgaaagatta cggcctgccc aagcctttgg aaatttattt aaacggtaat aggttagaat
    12661 aaacaaaata aacccattga actgttgttt gcaggtatcg cagcaagaac aaccgatgaa
    12721 tttgggtcgt attttaggcg gtgggataat gttcaaatgg gacatttgga acggaagaag
    12781 tcggcaattt aaaaaggatt taaaaagcaa agaaggtcaa aaacatgaac acaaacttaa
    12841 atgacaaaga caaagccatg gataccgcaa tcaggtttca gaaaaggatg aggattccga
    12901 aatttttctt tttaattctc tgaatcacaa tggttttggc atttatccaa gacgtgataa
    12961 cgggttctaa ttttctgcaa ataacaatta atgtaaaatt ttcgtaaaaa tttatcggct
    13021 tttaaaacaa aattgactaa aatagtcgcg agtttttact gcaataaagg agattgcaat
    13081 gaatatgaaa accttattag cactagcggt tagtgcagta tgttcagttg gtgttgcgca
    13141 agcacacgag cataatacga tacctaaagg tgcttctatt gaagtgaaag tgcaacaact
    13201 tgatccagca aacggtaaca aagatgtggg tacagtgact attactgaat ctaactatgg
    13261 tcttgtgttt acccctgatt tacaaggatt aagcgaaggc ttacatggtt tccacatcca
    13321 tgaaaaccca agctgtgagc caaaagaaaa agaaggtaaa ttgacagctg gtttaggcgc
    13381 aggcggtcac tgggatccta aaggtgcaaa acaacatggt tacccatggc aagatgatgc
    13441 acacttaggt gatttacctg cattaactgt attgcatgat ggcacagcaa caaatcctgt
    13501 tttagcacca cgtcttaaac atttagatga tgttcgcggt cactctatta tgatccacac
    13561 gggtggtgat aatcactccg atcatccagc tccacttggc ggtggcggcc cacgtatggc
    13621 atgtggcgtg attaaataat tcgattgttc gaaacgaaaa gtgcggtgaa ttttgaccgc
    13681 acttttttgc tagatattta gcatttaatt cacgacttca gcagtatagt gaattaaatt
    13741 taaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
    13801 gcaccaagtg aatcgattcc gtactatttg agacctttgc aaaattcctt tccctcccga
    13861 ca
//
LOCUS       DAXIHS010000053        13705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXIHS010000053 DAXIHS010000000
VERSION     DAXIHS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.053"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(69..2045)
                     /locus_tag="ACFOF2_001367"
     CDS             complement(69..2045)
                     /locus_tag="ACFOF2_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC7107676.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLKGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFOF2_001368"
     CDS             2194..3033
                     /locus_tag="ACFOF2_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC7107677.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALAQIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFOF2_001369"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFOF2_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC7107678.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4519..5244)
                     /gene="aat"
                     /locus_tag="ACFOF2_001370"
     CDS             complement(4519..5244)
                     /gene="aat"
                     /locus_tag="ACFOF2_001370"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC7107679.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5314..5748)
                     /gene="fur"
                     /locus_tag="ACFOF2_001371"
     CDS             complement(5314..5748)
                     /gene="fur"
                     /locus_tag="ACFOF2_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC7107680.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5967..6344
                     /locus_tag="ACFOF2_001372"
     CDS             5967..6344
                     /locus_tag="ACFOF2_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC7107681.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            6354..7163
                     /gene="dapB"
                     /locus_tag="ACFOF2_001373"
     CDS             6354..7163
                     /gene="dapB"
                     /locus_tag="ACFOF2_001373"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC7107682.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7427..7837)
                     /locus_tag="ACFOF2_001374"
     CDS             complement(7427..7837)
                     /locus_tag="ACFOF2_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107683.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7839..8084)
                     /locus_tag="ACFOF2_001375"
     CDS             complement(7839..8084)
                     /locus_tag="ACFOF2_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107684.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(8092..9264)
                     /gene="lpxB"
                     /locus_tag="ACFOF2_001376"
     CDS             complement(8092..9264)
                     /gene="lpxB"
                     /locus_tag="ACFOF2_001376"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC7107685.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9312..10304)
                     /locus_tag="ACFOF2_001377"
     CDS             complement(9312..10304)
                     /locus_tag="ACFOF2_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC7107686.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            10799..13555
                     /locus_tag="ACFOF2_001378"
     CDS             10799..13555
                     /locus_tag="ACFOF2_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC7107687.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTALETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALVYDAAEKIRRAAAILPEGATPKASAQEMPSETATFTAAAEQ
                     ARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEAATVPAEEMIQVE
                     TRQG"
BASE COUNT         3444 a         4450 c         3195 g         2616 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgcctttc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gctgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccaa ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgttgcatga cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggtc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc agatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttggc agtgattttg cccggctgtc gtcatttcta attttatagt
     4381 ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg
     4441 gcttcgtcgc cttgtcctga tttttgttaa tccactataa tccaccatat ttgaacctga
     4501 ccgaagcata agccgccatc aaagcccctt gcacgccact tcgcgccgcc cgatttcctc
     4561 tttcaacggc acggcgttca acatccgcag acgttcggca aaatccgcaa acggcagcag
     4621 ctccgaaccg aaacggcgca tatgttccgt gtcctgctgg cagtctatca gttccacacc
     4681 caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt ttcgacgcat ccggttgcaa
     4741 cgcaaacatc gattcgccat aaaacacgct gccgatctgc acgccgtaaa agccgcccgc
     4801 caacctcgtt tcaccgcttt catcgggata atggcactcg aaagaatgcg cgtgtcccat
     4861 ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca atccaagttc cgtcctgatt
     4921 cgggcgcgcc gctgccgcac aatgcgcgac cacttccgca aaacagccgt tgaccgcaac
     4981 ccgatagctg ccgttgcgca gcgttttcgc cagcgagcgc ggaacgtgca gacgttcggg
     5041 aacaatcacc gcacgcgggc cgaccgcata ccaaaaaaac caaccgtccc gagaaaacca
     5101 cggaaacacg ccgttccgat acgcctcgag cagccgcccc gcatccaaat cgccgctcac
     5161 gccgaccagc ccgtcgcacc gggccaaagc ataggcagga tcgggaaagg cataattgtc
     5221 aggggcaagc agcggaatac gcatggcagt tctccaaaaa cacagacggc atccaaaatc
     5281 atgaatgccg tctgaacaac aaaccgtccg gatttaacgt ttgcccttgg cctgacagtc
     5341 gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg cggtagccgt tttcctcagc
     5401 gattttgtct tgcagggctt cgatttcggg attgtggaat tccgttacct cgccgcactt
     5461 cacgcagacg atatggtcgt ggtggtcgcc cttgtccaac tcataaaccg ccttgcccgt
     5521 ttcaaaatgg tggcgttgca gaatgcccgc ctgctcaaac tgggtcagca cgcggtaaat
     5581 cgtcgccaca ccgatttcca caccctcttc caacaaaatg cggtacacat cttccgcact
     5641 caaatgctct tccgcatgcg tctcgaacaa atccaaaatc ttcaaacgcg ggccggtaac
     5701 cttcagaccg ctgtctttca gttgtgcaat attgttgaat ttttccataa tattcaatac
     5761 ccctgtaaaa caatagacgt tataatacgc aatttcggcc tgtttgccca ctatcgcacc
     5821 atagcagttt gcaatagtaa aaccgcaccg gcggccgatg cgcggacagg gcggataccg
     5881 cttaatcgta tgattatcgt tcgcttttat aaaatattca agcagtgtta cactacccat
     5941 cccgatttgc acagaaaggc atttccgtga acaaaaccct catcctcgcc ctttccgccc
     6001 tcctcggcct tgccgcgtgc agcgtcgaac gcgtttcact gttcccctcg tacaaactca
     6061 aaatcataca gggcaacgaa ctcgaacctc gcgccgtcgc ctccctccgc cccggtatga
     6121 ccaaagacca agtcctgctc ctgctcggca gccccatact gcgcgacgca ttccataccg
     6181 accgctggga ctataccttc aacacctccc gcaacggcat catcaaagac cgaagcaatc
     6241 tgaccgtcta ttttgaaaac ggcgtgctcg tccgcaccga aggcaacgcc ctgcaaaatg
     6301 ccgccgaagc cctccgcgta aaacaaaacg cagacaaaca ataaggaaca cacatgacac
     6361 cgctcaaaat cgccatcgcc ggcgcaaacg gccgtatggg gcgcgtcctc gtcgaagccg
     6421 tcaacaacca tcccgacacc gtcctttccg gtgcgcttga acactcaggc tcagaagccc
     6481 tcgggctgga cgcaggctac gccgtcggac tcaaaaccgg catcgccatt tcagacgacg
     6541 ttgacgccgt tctcgcacaa agcgacgtac tcatcgactt cacccgcccc gagcccaccc
     6601 tcaaacacct gcaaaaatgc gttgaaaaac aagtcaacat catcatcggc acaacaggct
     6661 tcgacgatgc gggcaaagcc gctatccaca ctgccgccga acaaacaggc atcgttttcg
     6721 ccgccaactt cagcgtcggc gtcaacctca ccttccacat cctcgacacc gtcgcccgcg
     6781 tgttaaacga aggctacgac atcgaaatca ttgaaggcca ccaccgccac aaagtcgatg
     6841 cccccagcgg caccgcgtta cgcatgggcg aagtcatcgc cggcgcgctc ggcagagacc
     6901 tcaaacaatg cgccgtttac ggccgcgaag gccacaccgg tccgcgcgat ccgtcgacca
     6961 tcggctttgc caccgtccgc gcaggcgaca tcgtcggcga ccacaccgcc ctcttcgcca
     7021 ccgacggcga gcgcgtggaa atcacccaca aagccggcag ccgcatgacc tttgccgccg
     7081 gcgccgtccg cgccgcagtt tgggtaaacg gcaaaacggg tttgtacgat atgcaggacg
     7141 tactcgggct gaacaaccgt taacccccat acaaaatgcc gtctgaaaag atattgttca
     7201 gacggcattt tgccgacagg ctccgtatcg gcatatcaat gtttcaacac acgggacgat
     7261 acataaagcg tcgccctatg tgtcgtcctg atttggaagg gattacgccc ctcccaaata
     7321 aagtctgatt ctaccgcccc gaaggacaga tgtccgagtg gcggggtttc aaccgaaaag
     7381 gaaatacgat aaagttgctt gcgcggcatg acaagttacg gctcgtttaa ttaaaactct
     7441 cctgatctaa aaattctaac tctatttccc ggcaaactat atctatacta aaaccggtcg
     7501 gaagatcata cctaccgcct atcacttcca gcgcacctcc ataatccaag atttcatcat
     7561 cctgctttaa ttctccaagt tccaatacat ttttgatatt tttatcttgg ttcttcgtgg
     7621 cgtatccata gataaatttc agatagctga actgcttgaa tttaattttt acccattctg
     7681 aataattggg catagggatc tcttgaggaa agtccggata atccacattc tggacgatga
     7741 tatggaattc atcgtttttc aaagtatagt ttaatagagt gccctcatat atatacagaa
     7801 attgataatc tttttttgga atggtataag ttgtcatatt attttacctt ttcaaaaaca
     7861 atgagtttaa gattttttga gtccaagttg gttctactac tcttatgaaa tttcagttgt
     7921 ttaaactctt cctttaacag accattatat tttaacctta atcccactcg agccagttct
     7981 gcttcatttg gaattcctct taaatactta ttgtttgatg tgccattaat cacaatttct
     8041 cccccttttt ttgtaatacg tgctgcttcg ggaaaataat ccattaaccg ctcaacatcc
     8101 cgcctcttcc aaaaccgcgc gcgcggccaa atccgccgta tcttttttca acagcaggtg
     8161 caacgccctg aaatcctgtt gcagcgcggc aaccttatcg gggtgttcgt accagtccgc
     8221 caacgccgcc gccagttttt ccggttttgc ttcagattgc aataattccg gcacagcctc
     8281 cttacccaac aggatattcg gcaggccgac atgcggcact ttgattttgc gtttcacata
     8341 agcataagtc agcggcgaaa tcttgtagct gatgaccatc ggacgcttgc acaacgccac
     8401 ctccaaagtc gccgtaccgc tcgttaccag caccgcatcc gccgccctgc acactgtttc
     8461 agactgtctg tcgattaccg tcagcggcaa tccggcaaac tccggccgct gcaaaacttc
     8521 cgccaaacgc cgcttcgtcg cctccgttgc ggcaggcagc aggaagcatg cggcaggata
     8581 gcgttccaac aacaataatg ccgtctgaaa aaacaccggc gccatatagt cgatttcgct
     8641 gacgcggctg ccgggcagca gggcgaatac ggggatgccg gcatccacgc ccaaagtttg
     8701 ccgcgccgtt tcacggtcgt cttccaaggg cataagctgc gccatcggat gaccgacaaa
     8761 ctccgcacgt ccgcccgcat cgagataaag ctgcggctcc atcgggaaca ggcacaacac
     8821 gcggttgacc tgatgcacga ttttgcccac acgttcccgc cgccacgccc acaccgacgg
     8881 gctgacataa tgcacggtcg gaatccccga ccgtttcagc ttttccgcca cacccaaatt
     8941 aaaatcgggc gcatcgatac cgacaaagac atcaggtttc aacgacagca aatcccgtac
     9001 cagccccctg cgtatccgta aaatttccgg cagccgcctg accacttcga caaagccgcg
     9061 caccgccagc cgctcctgat cataaaggct ctcgaaacct tccgccttca tcagttcgcc
     9121 gccgataccg acaaaacgcg cctgcggaca acgcttgcgg atggcgcgta tcaggtgcgc
     9181 ccccaatagg tcgcccgacg cttcgccgac actgacggca atcaaagggc tttttttatc
     9241 agccatattc gtctgtcccc acatactttc acgtcccgat gccgtctgaa ggcttcagac
     9301 ggcatcgggg ttcacatttt cgtcccgttt tccaacatca ccgcaaactg cgccaagtcc
     9361 ggcggcagct ccgccttcaa caccagcggc tcgcccgtga gcggatggtt caagtgcagc
     9421 tcggacgcgt gcaaaaacat ccgcttcaaa cccaacttct gcaaacgacg gttcgcctga
     9481 taatcgccgt agcgttcgtc gcccgcaatc ggacagcctt gagattgcag gtggacgcgg
     9541 atttggtgcg tacgacccgt tttcagcgtt gcccgcacca aagtcaggtg cgacagcccg
     9601 acaccgtgca aaatgccgtc tgaaaaacgg cttaacacac ggaacaccgt gtgcgccgac
     9661 tgcccgtcct cgctgacgcg taccatcttt tcgccttgtg cgccggtata tttgaacagg
     9721 ggcagtttga catggaaatt gtcgtccggc agtttgccca cccccagcgc aaggtagatt
     9781 tttttggggt ggtcgttacg gatggcttcg tgaagtttga cgagcgcgct gcgtttcttc
     9841 gccaccatca acaagccgct cgtatccttg tccaaacgat gaaccaactc caaatacttc
     9901 gcctccggac gggcgcggcg caactgttcg ataacgccga aactcacgcc gctgccgccg
     9961 tggacggcaa cgccggacgg tttgtcgatg accaaaagcg catcgtcttc gtaaacaacg
    10021 tcaaacgcac gcgccggtac ggcggcacgc ctttcagacg gcatttcctt ctccgccacg
    10081 cgcacaggcg gaatccgcac cgtatccccc tccgcaatac ggctgtcggg tttgcagcgt
    10141 ttcttgttca accgcacctc gccggcgcgg ataatgcggt ggatatggct cttgggaaca
    10201 cccttgagga tttttatcag atagttatca aggcgttgac ccgcctcatg ttcggcaacc
    10261 cctatcaagc tgaccgaatc tttgcttatt tcgtgcgttt tcatctataa tccgaacatc
    10321 cgtttcagca aaaagcgcgc ccgccgcgcc cccctgaaac ggtttacttt aaaacggaat
    10381 tttatattaa aaacgcactc cgcaaagcat tttccctgcg cctgttccca gccggcaggc
    10441 gcagaacgta atcaagtttg aattgatttt gccgtttcgg cgcggaagtt aagacggcag
    10501 ccgggcccca agtcccaaac gcaaaccgcc caaaacacag gcatcacgat aacaagaaga
    10561 agccggcccc catttttccg accgcagcgt tcggaacgcg gcagacaaac cgccgcatcg
    10621 atctttatag ccttctgtat ccgacccttc cgcacacggt tctccggaac gtgcatccct
    10681 gcggattttc aactcaaaaa ctgattaccg gttttatccg aaaacatcgg aaaacccagc
    10741 cttacggaat gccgaaccgg accggcgcgc ccgaatgccg cccgtccacg aggtgatcat
    10801 gaaaagaatg ttattcaacg caacgcaggc cgaagagctg cgcgttgcca tcgtcgacgg
    10861 acaaaacctg ctggatttgg acatcgaaac gctgggcaaa gaacagcgca aaggcaatat
    10921 ctacaaaggc atcattaccc gcatcgagcc gtcgctggaa gcgtgtttcg tggattacgg
    10981 aaccgaccgc cacggtttct tgccgtttaa agaagtctcc cgctcatact tccaagacta
    11041 cgaaggcgga cgcgcgcgca tccaagacgt gctcaaagaa ggcatggaag tcatcgtcca
    11101 agtcgaaaaa gacgagcgcg gcaacaaagg cgcggcgctg accaccttca tcagcctcgc
    11161 cggacgctat ctggtattga tgccgaacaa cccgcgcggc ggcggcgtat cccgccgtat
    11221 cgaaggcgaa gaacgtcaag aactcaaagc cgctatggcg caactcgaca ttccgaacgg
    11281 catgagcatc atcgcccgta ccgccggcat cggccgcagc gcggaagagt tggaatggga
    11341 tttgaactat ctcaaacaac tctggcaggc gattgaagaa gcaggaaaag cgcatcacga
    11401 cccttacctg ctctttatgg aaagctcgct gctcatccgc gccatccgcg actatttccg
    11461 ccccgacatc ggcgaaattc tggtggacaa tcaagaagtt tacgaccaag ttgccgagtt
    11521 catgagctac gtcatgccgg gcaatatagg ccgtctgaaa ctctacgaag accacacgcc
    11581 gctgttttcc cgcttccaaa tcgaacacca aatcgaaagc gcgttttctc gcagcgtcag
    11641 cctgccttcc ggcggcgcaa tcgtcatcga ccacaccgaa gccctcgtct ccatcgatgt
    11701 caactccgca cgcgccactc gcggcgcaga cattgaagac accgcgttca aaaccaatat
    11761 ggaagccgcc gaagaagtcg cgcgccaaat gcgcctacga gacttgggcg gtttggtcgt
    11821 catcgacttc atcgacatgg aaaaccccaa acaccagcgc gatgtggaaa acgtcctgcg
    11881 cgacgcgctc aaaaaagacc gcgcccgcgt gcagatgggc aaactctccc gcttcggcct
    11941 tttggaattg agccgccaac gcctgaaacc agctttgggc gaaagcagcc acgtcgcctg
    12001 tccgcgctgc gccggcaccg gcgtgattcg gggcatcgaa tccaccgccc tgcacgtttt
    12061 gcgtatggtt caagaagaag caatgaaaga caacaccggc gaagtgcgcg cccaagtgcc
    12121 cgtcgatgtc gccaccttcc tgttaaacga aaaacgcgcc gagctgtttg cgatggaaga
    12181 gcgtttggat gtcaacgtcg tcctgattcc gaacatccac ctcgaaaacc cgcactacga
    12241 aatcaaccgc atccgcaccg acgacgtaga agaagacggc gaaccgagct acaagcgcgt
    12301 cgccgagccg gaagaagacg aatccgccaa acctttcggc ggcgaaaaag ccaaagccgc
    12361 ccgtcccgaa cccgccgtca aaggcgtgcg ccacaccagc cccgccccga ctgccgcccc
    12421 cgagaaaaaa acctcctggt gggacagctt caaagcatgg ctcaaacgca ttttcggcgg
    12481 cagcgaaacc caagccgctc ccgctgccga aacctccgaa aaacgcagca cggcaaaccg
    12541 cagcggcagc cgcgccaaca accgccgcca aaacccgcgc cgcagcaaac gcgaaggcag
    12601 caaagtagaa gtccgcgaag tggcaggcaa aactgccgga caggaagcgc gtgccgacaa
    12661 agccgaaacg cgcaacaacg gcaaccgccg ccgcaacgaa cgcggcgacc gtgccgccga
    12721 acgcgccaac gaagcggaaa tccaagaccg caacatacag cctgccgcaa ccgttgcaga
    12781 tgccgcaccg tccgaaaccg aagtgcaaac cggcaaacgc cgccgcaacg gcagccgcaa
    12841 cgaacgcggc caaaccgcgc tggaaaccgc cgccgttgcc gaaacagccg ttcagacagc
    12901 ggaaaacacg ccgtccgaac cgcataccgc agaagacaaa ggcagcaagc ccaaatccga
    12961 acgcaaccgc cgcgaacgcg acagccgcga cgccaaagaa cgccgcgagc gcaacaatca
    13021 gcgcgaccgc cgtcaaaacg gcaaaaaacg caatatcccg tctgccgcca aaatcgagca
    13081 gtacctgaat attcacgaca ctgccgacaa agtccgttcc gccgccgcac acgttttcgg
    13141 agaaaccgac gcaaacgcgc cgattaccgt cagcattgcc gatccgctca tcgccacacc
    13201 cgttcagacg gcatcttccg ccgtttcaaa cggcgacgca ctggtttatg atgcggcaga
    13261 aaaaatccgc cgtgccgccg ccatcctgcc cgaaggcgcg acaccgaaag cctcagcaca
    13321 ggaaatgccg tctgaaaccg caacctttac ggctgcggcg gaacaggcac gggaaaccgc
    13381 acaaaccggc ggactcgtcc tgatcgaaac cgaccctgcc gcattgaaag catgggcggc
    13441 acaacccgaa gtccaaaccg gacgcggttt gcgccgttcc gaacagccca agccgtctga
    13501 agccgcaacc gtccctgccg aagaaatgat ccaagtcgaa acccggcaag gctgaaccga
    13561 cggcggcaaa aagaggttct gttccgcaga acctcttttt tatatagatt cggatacctg
    13621 caatgccgtc tgaaacttcg ccattcccgt gattaccgaa acattccgcc attcccatga
    13681 ttcccgcaac attccgtcat tcccg
//
LOCUS       DAXIHS010000054        13497 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXIHS010000054 DAXIHS010000000
VERSION     DAXIHS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.054"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..194
                     /locus_tag="ACFOF2_001379"
     CDS             <1..194
                     /locus_tag="ACFOF2_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107688.1"
                     /translation="LGVIRSENRRKPVFGFRLSGGKGILQRSHKVFATFGDKRTTRLF
                     ADLDALAEVMEKTISENKK"
     gene            205..1175
                     /locus_tag="ACFOF2_001380"
                     /pseudo
     CDS             205..1175
                     /locus_tag="ACFOF2_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(1226..2278)
                     /gene="sohB"
                     /locus_tag="ACFOF2_001381"
     CDS             complement(1226..2278)
                     /gene="sohB"
                     /locus_tag="ACFOF2_001381"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC7107689.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2382..2471)
                     /locus_tag="ACFOF2_001382"
     tRNA            complement(2382..2471)
                     /locus_tag="ACFOF2_001382"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2435..2437),aa:Leu,seq:taa)
     gene            complement(2574..2647)
                     /locus_tag="ACFOF2_001383"
     tRNA            complement(2574..2647)
                     /locus_tag="ACFOF2_001383"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2613..2615),aa:Cys,seq:gca)
     gene            complement(2673..2748)
                     /locus_tag="ACFOF2_001384"
     tRNA            complement(2673..2748)
                     /locus_tag="ACFOF2_001384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2713..2715),aa:Gly,seq:gcc)
     gene            complement(2770..2845)
                     /locus_tag="ACFOF2_001385"
     tRNA            complement(2770..2845)
                     /locus_tag="ACFOF2_001385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2810..2812),aa:Gly,seq:gcc)
     gene            complement(2869..2944)
                     /locus_tag="ACFOF2_001386"
     tRNA            complement(2869..2944)
                     /locus_tag="ACFOF2_001386"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2909..2911),aa:Gly,seq:gcc)
     gene            complement(2970..3045)
                     /locus_tag="ACFOF2_001387"
     tRNA            complement(2970..3045)
                     /locus_tag="ACFOF2_001387"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3010..3012),aa:Gly,seq:gcc)
     gene            complement(3125..3688)
                     /gene="pgsA"
                     /locus_tag="ACFOF2_001388"
     CDS             complement(3125..3688)
                     /gene="pgsA"
                     /locus_tag="ACFOF2_001388"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC7107690.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3794..4217)
                     /locus_tag="ACFOF2_001389"
                     /pseudo
     CDS             complement(3794..4217)
                     /locus_tag="ACFOF2_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4351..4416)
                     /locus_tag="ACFOF2_001390"
                     /pseudo
     CDS             complement(4351..4416)
                     /locus_tag="ACFOF2_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4549..4884)
                     /locus_tag="ACFOF2_001391"
     CDS             complement(4549..4884)
                     /locus_tag="ACFOF2_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC7107691.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            5013..5918
                     /gene="mtrA"
                     /locus_tag="ACFOF2_001392"
     CDS             5013..5918
                     /gene="mtrA"
                     /locus_tag="ACFOF2_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC7107692.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(5980..6468)
                     /locus_tag="ACFOF2_001393"
     CDS             complement(5980..6468)
                     /locus_tag="ACFOF2_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC7107693.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6615..7517
                     /locus_tag="ACFOF2_001394"
     CDS             6615..7517
                     /locus_tag="ACFOF2_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC7107694.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7558..8688)
                     /locus_tag="ACFOF2_001395"
     CDS             complement(7558..8688)
                     /locus_tag="ACFOF2_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7107695.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            8893..11523
                     /gene="alaS"
                     /locus_tag="ACFOF2_001396"
     CDS             8893..11523
                     /gene="alaS"
                     /locus_tag="ACFOF2_001396"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC7107696.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARARAAQSFKANAQLPY
                     DGQDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDV
                     GYIFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSA
                     THLMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANV
                     AVNAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKI
                     ISEGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHA
                     KALEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSD
                     NAVILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTD
                     AGKLPEMLVSVESWLCQKLS"
     gene            11585..12316
                     /locus_tag="ACFOF2_001397"
     CDS             11585..12316
                     /locus_tag="ACFOF2_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107697.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            12623..>13497
                     /locus_tag="ACFOF2_001398"
     CDS             12623..>13497
                     /locus_tag="ACFOF2_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC7107698.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWR"
BASE COUNT         3411 a         3519 c         3236 g         3331 t
ORIGIN      
        1 aattaggagt aattaggagc gaaaatagac gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcaca aagtatttgc cacattcggc gacaagcgca
      121 caacccggct gtttgccgat ttggatgcac tggctgaagt gatggaaaaa acaatctcgg
      181 aaaataaaaa ataggggggc aaatatgtgg aaaatgttga aacacatagc ccaaacccac
      241 cgcaagcgat tgattggcac attttccctg gtcggactgg aaaacctttt gatgctggtg
      301 tatccggtgt ttggcggccg ggcgatcaat gccgtgattg cgggggaggt gtggcaggcg
      361 ttgctgtacg ctttggttgt gcttttaatg tggctggtcg gtgcggcgcg gcggattgcc
      421 gatacacgca cgtttacgcg gatttatacc gaaatcgccg tgccggttgt gttggaacag
      481 cggcagcggt aagtcccgca ttcggcaata tagtggatta aatttaaacc agtacggcgt
      541 tgtctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt
      601 gttaatccac tataactgcg cgggttgccc tgtcgcgtga gtttgtcagc ttttttgaag
      661 aacacctgcc gattgccgcg acatccgtcg tatccatatt cggcgcgtgc atcatgctgc
      721 tggtgctgga attttgggtc ggcgtgtcgg cggtgggcat acttgcgttg tttttatggc
      781 ttttgccacg ttttgcggcc atcagcgaaa acctgtattt ccgcctgaag aacagcttgg
      841 aacgcgacaa ccactttatc cgaaaaggcg accggcggca gctgtaccgc cattacggac
      901 tgcttgcgcg cctgcgtgtg ctgatttcca accgcgaagc cttcggctat ctctgcgtcg
      961 gcacggcgat gggtattttg ttcggctttg cttttgtgat gatgacgctc aaaggctaca
     1021 gcagcgcggg gcatgtctat tcggtcggca cttatctgtg gatgtttgcc atgagtttgg
     1081 acgacgtgcc gcgattggtc gaacaatatt ccaatttgaa agacatcgga caacggatag
     1141 agtggtcgga acggaacatc aaagccggaa cttgaaaaat gccgtctgaa cacgcttcag
     1201 acggcatttc catccgttcg gcaaactaca tcacatccgc ccgccggttg acaagtttgg
     1261 caaacaactt ttcaacagaa gcttccgcct gcaaaccaat gcgctggatc aggctttgct
     1321 tctcctgata tttcacttcg ataacctgtt tgttttcaaa cgctttcaac aacaaatcat
     1381 cactggtcga aatctcgtca atcaagttca acgccaacgc ctgccgaccg aaccaatgct
     1441 cgcccgttgc cacttcctca atatccaatt gagggcggtt ctcgctgaca aactgcttga
     1501 acaactgatg cgtttcctcc agttcctgtc ggaatttctg tttgcccttt tccgtatttt
     1561 cacccataaa agtaaccgtg cgcttaaatt cgcccgccgt catcacatcc acatcaatat
     1621 catgtttttt caacaggcgg tggatattcg gtacttccgc caccacaccc accgaaccga
     1681 caatcgcaaa cggagcggaa acaattttat ccgccacaca cgccatcata taaccgccgc
     1741 tcgccgccac cttatcgacg gcgacggtca gcggaatatt gcgttcgcgc aaacgcctaa
     1801 gctgtgaagc cgccaaaccg taaccgtgaa ccacgccgcc cggactttcc aatctaagca
     1861 gaacctcatc ttcaggcttg gcaatcaaaa gcaccgccgt aatctcatga cgcaaggatt
     1921 ctacggcgtg tgcatacaaa tcgccgtcaa aatccaacac aaaaaggcgg gatttttgcg
     1981 tttcggcaga tttctcccca ccctccttca aacgcttttt ctctgctttg gcttccgcct
     2041 tttccttttt cttttcctct ttttcctgat gttttgcctc ttccccgctt aaaaagaatg
     2101 cttcaaacga ttgccgctgt tttttataat tttccgaaaa atccgtcagt acgacactgc
     2161 cgctttccga ctgtttctta ctctgtacga tagccaacac aatcagcgca attgcgccga
     2221 acacggtaag cagttcgagc aggaaaatac cgtaattcag taaaatttct ttccacattg
     2281 attggatttc ctcttgttca ggcatgaaca tgtcaatatt gtccatcacc gtccgacaga
     2341 taaaaaaata accgcttgga gcggcattgt cattttcagc ttggtgcccg gagccggaat
     2401 cgaaccggca cgggatgttt agtcccgacg gattttaagt ccgttgtgtc tacctatttc
     2461 accacccggg catttgtgaa aggtggaggc gggggcgcgg attttaaccg gcctatatga
     2521 agattgcact cctcatagca taaacactct gccaccccgc catagtacga taatggaggc
     2581 gagagtcgga atcgaaccgg cgtagacgga tttgcaatcc gctgcataac cactttgcta
     2641 tctcgcccta aaactggctt atctaaaaaa cttggagcgg gaaacgagtc tcgaactcgc
     2701 gacctcaacc ttggcaaggt tgcgctctac caactgagct attcccgcgc gttcaaacat
     2761 atcggttttt ggagcggaaa acgagtctcg aactcgcgac ctcaaccttg gcaaggttgc
     2821 gctctaccaa ctgagctatt cccgcgttga tatgtttgaa ataaaacttg gagcgggaaa
     2881 cgagtctcga actcgcgacc tcaaccttgg caaggttgcg ctctaccaac tgagctattc
     2941 ccgcaatgat tgtgggaaaa tgaaattttt ggagcgggaa acgagtctcg aactcgcgac
     3001 ctcaaccttg gcaaggttgc gctctaccaa ctgagctatt cccgcccgat ttcattctcc
     3061 aacatcgaag agacgcaatt attatggatt ctgtttttgc cgtcaagcta tttttatgtt
     3121 tttttcaggc gatttctttc cacgccattt tcagataata cagcatcgac cagactgtca
     3181 gcaaagatgc gataaacatc aatacattgc cgataacaac aagattaaat ccgtaaaaat
     3241 cgggaatatt cagcagcagc aggaagattg ccagcatttg cgcggcggtt ttgaacttac
     3301 cgacggtggc gacggcaacg ctgttccttt tgcccatttg cgccatccat tcgcgcaatg
     3361 cggaaatggt aatttccctg ccgataatga tcatggcaaa caaaacatag gtccggtcga
     3421 gtttgaccag taaaagtagc gatacggcaa ccatcagctt gtcggcgacg ggatcgagga
     3481 aggcgccgaa atcggaagtc tgtttccaca gtcttgccaa aaatccgtca aaccagtcgg
     3541 ttaaggcggc aacggcaaaa atgacggcgg cggtgagatt aatcgtttcc tccgcgaacc
     3601 acggaaaagg caggtaaaaa agggctgtca tgacaggaat gagcaagacc ctcaaccatg
     3661 tgaggaagat ggggagattc caaggcatcg attttctctg tgcagactgt aaagttgtga
     3721 ttataacggt tatcctcata acccaaaacg taaaattgct gcatgggcat tcccccgccc
     3781 cgccaatctg ttttcacatt cttttcaaac gcaggaaaat ggcgggcaat aaaagcaaaa
     3841 tacccagttt caggctgaaa acggcaggtt gtgccaacac ttcgacaagg cggtcttccg
     3901 tgcgggcaaa atctttattg cttatagaca ctgccactgt tgcggtattc caacagaacg
     3961 ccgtttaaaa aacctttgcc gacggtttcg cttaaaacgg ctctaacctg ctccgccctg
     4021 atggttctgc cgatattgcc gcctgtgcac aaactgtcga acccatagca ggaaagccgg
     4081 taatgctgcc cgtctgcatc cagtttgatt gcccgtccgc tgcggttggg ggcggtaacg
     4141 gtcaattccg catattctaa tgttttttct tgttcgtgaa atgccgtcag gtaaggtgca
     4201 ataaaaacgg cggacaacag cagacagctt atggcggcaa accataccca gcgataatat
     4261 agtggattaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag aacgattctc
     4321 taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt
     4381 cgccttgtcc tgatttaaat ttaatccact atatttcacg cttacccctt gtttctcaaa
     4441 tgccgtctga aaaaaacggc tgtatatgtt gtctgcaaaa tatcgggaag cataacagac
     4501 aaaatgccgt ctgaaatatt ttcagacggc atttcaatcc gattcggatt atttttgcgt
     4561 ttcaaccgct tccaatgcac gcagggcgta agtgtaagcg gcaccggcat tcagggcgat
     4621 ggcggttgcc aatgcgccgg cgatttcgct gtcggtcgca ccggctttgg cggcggcggc
     4681 ggcgtgaaca ctgatgcagc tctcacaacg tgtggtaatg gcaacggcga tggcaatcag
     4741 ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc tgttccaatg cgccgtaggc
     4801 ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac gattttttaa ccaatgcggt
     4861 atgttctttc caatctttaa acattttctt ttcctttctc ttgcgtttaa ccctgatacg
     4921 cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt attcagttgt gtttctcgtt
     4981 taatcatctc attttatggt tcaaaaagat ttatggacat tctggacaaa ctggtcgatt
     5041 tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt gggcggacaa tggtcggtac
     5101 ggcatgaaac cttgcaacgc gaaggattgg tacacattgt tacatcgggc agcggctatc
     5161 tctgcatcga cggcgaaact tccccgcgtc cggtcagtac aggggatatt gtatttttcc
     5221 cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg cggagaaagt ttacaaccgg
     5281 atatgcggca gcacggtgcg tttacggtca aacagtgcgg caacggacag gatatgagcc
     5341 tgttttgcgc ccgtttccgc tacgacaccc acgccgattt gatgaacggg ctgcctgaaa
     5401 ccgtttttct gaacattgcc catccgagtt tacagtatgt ggtttcaatg ctgcaactgg
     5461 aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa cgcattgtcg tccgtcctgc
     5521 tggtgcttat cctgcgcgcc tatctcgaac aggataagga tgtcgaactc tcgggcgtat
     5581 tgaaaggttg gcaggacaaa cgtttgggac atttaatcca aaaggtgata gacaaaccgg
     5641 aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa tatgtcgcgc gcgcaactga
     5701 tgcgccgttt caaaagccgg gtcggactca gcccgcacgc ctttgtgaac catatccgcc
     5761 tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc ggttttgtcg gtcgcactgt
     5821 cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt caaacggcaa tatcacgttt
     5881 cgccgggtca ataccggaaa gaaggcgggc aaaaataaat cggggcttca aacgcaaatg
     5941 ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt catttcaagg gctgcatctt
     6001 catcacttcc atcaaaaagt tggtaaatgc ggggttgttg ggtttgacat ccatattttt
     6061 ccaacgctgc tgccagccgc gcaaggcatt ctggatatac agcttggact gttccgtatt
     6121 gattgcgccc cgctggctgt ctatcgccga acgcaggtag atttcataca tactgtcatc
     6181 gacggcattg cgtccgacca ggcgttttct gaagttgttc agatattgcg ccgcctgaac
     6241 cttggtcatt ttaccgatac ccacctgata gcccaagcgc gtcgcttcat cgctgatttt
     6301 ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct tttgcaggtg cttccgtttt
     6361 gacggtattg ataggattca cggggatttc cgtcaatgtg ggcacataaa tagactggca
     6421 gccggaaaga actgccacaa tggaaagagg gataaggtat tttttcatgc ccccattata
     6481 atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa aataaaattt cggcatcaga
     6541 agcaggcaaa aacacattcc acaagccttg ccgcaaggtt tacaatccga ccgtccttat
     6601 cgcaacgacc gtttatggat accgcaaaaa aagacatttt aggatcgggc tggatgctgg
     6661 tggcggcggc ctgctttacc attatgaacg tattgattaa agaggcatcg gcaaaatttg
     6721 ccctcggcag cggcgaattg gtcttttggc gcatgctgtt ttcaaccgtt gcgctcgggg
     6781 ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca ttggaaaaac cacttaaacc
     6841 gcagtatggt cgggacgggg gcgatgctgc tgctgtttta cgcggtaacg catctgcctt
     6901 tggccaccgg cgttaccctg agttacacct cgtcgatttt tttggcggta ttttccttcc
     6961 tgattttgaa agaacggatt tccgtttaca cgcaggcggt gctgctcctt ggttttgccg
     7021 gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca ggaaacggcg gcactcgccg
     7081 ggctggcggg cggcgcgatg tccggctggg cgtatttgaa agtgcgcgaa ctgtctttgg
     7141 cgggcgaacc cggctggcgc gtcgtgtttt acctttccgt gacaggtgtg gcgatgtcgt
     7201 cggtttgggc gacgctgacc ggctggcaca ccctgtcctt tccatcggca gtttatctgt
     7261 cgtgcatcgg cgtgtccgcg ctgattgccc aactgtcgat gacgcgcgcc tacaaagtcg
     7321 gcgacaaatt cacggttgcc tcgctttcct atatgaccgt cgttttttcc gctctgtctg
     7381 ccgcattttt tctggccgaa gagcttttct ggcaggaaat actcggtatg tgcatcatca
     7441 tcctcagcgg tattttgagc agcatccgcc ccactgcctt caaacagcgg ctgcaatccc
     7501 tgttccgcca aagataaaaa atgccgtccg aacatccttc agacggcata tcgggcttta
     7561 tttccccgcc ttcacatcct gccactggcg caccataaac ttcaatgccg ccggctggat
     7621 aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt gggaaaatcg tattgtcgtt
     7681 tttaaattcg tcttccatca gctcacgcgc aggcttgctc gaaggcgcgt aagtaacgaa
     7741 attgccgttt ttcgccgaca cttccgggtc gaggaagtcg ttgatgtatt tgtgcgcgtt
     7801 ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc acccaaatcc ccacgccctc
     7861 tttgggcatc atcacgcgga ttttttcctt gccgcccgct tcttcggcac ggcgtttggc
     7921 gatgttcaaa tcgccgccga aaccgattgt tacgcaggta tcgccgcgcg ccaaatcatc
     7981 gataaagccg gacgaagtaa agcgtttgat attggggcgg tttttcttga gtagggcggt
     8041 tgcctccctg atgtcttccg tattgctgct gttcgggttt ttacccaaat agttcaacac
     8101 cataggatag atttccgccg cgctgtccaa atagctgatg ccgcattgct tgagtttgga
     8161 cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc ggcagcttgt ccgtacccaa
     8221 agcctttttc acgcgttcgg tattgatggc gaaggtattt gtcccccaat aaaacggcac
     8281 ggcgtattcg tggccgggat cgaccccgtc catcagcctc atcatttcgg ggttgaggtg
     8341 tttataattg ggaatcagcg acttatcgat tttctgatac gcacctgcct taatctgcct
     8401 gcccacaaac gcattggacg gcgcgacaat gtcgtaacca gattttccgg tcagcacctt
     8461 gctttccagc gtttcatcgc tgtcgtacac atcataagta accttgatgc cgtttttctt
     8521 ttcaaaatcg gcaacggttt ccggatcgac gtattccgac cagttgtaaa ttttcaatac
     8581 gtttcggttt tccgccggcg ccggtttttc ggcaggcggt ttgtccgaac cgccgcacgc
     8641 tgcaagcagc aaagcagtca ggacggccag gggcagatgt ttggtcatta tcattccttg
     8701 catatcgggt tggagaaagc ggccattata gccgatattg gcaacagggc ttcagacggc
     8761 attcaaaatc ccgccacact cttccgaaaa ccgccgcttc catagctaga aacagggatt
     8821 tgcggtaaga taccgccgtt cgttttccct gcttttacca tgacaagaca tttgagagac
     8881 attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat tcctaaaatt ttttgaaacc
     8941 aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc acgacgaccc gaccctgctg
     9001 tttaccaacg cgggcatgaa ccagtttaaa gacgtattct taggtttcga caaacgcccg
     9061 tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag gcggcaagca caacgacttg
     9121 gaaaacgtcg gctacaccgc ccgccaccac accttctttg aaatgatggg caacttctcc
     9181 ttcggcgact acttcaaacg cgacgccatc cacttcgctt gggaatttct gacttccccc
     9241 gaatggctca acatccctaa agacaaactg ttggcgaccg tttacgcgga agacgacgaa
     9301 gcctacaaca tctggttgaa cgaaatcggt atgccgtccg agcgcatcgt ccgcatcggc
     9361 gacaacaaag gcgcgaaata cgtgtccgac aacttctggc aaatgggcga caccggccct
     9421 tgcggcccct gctccgaaat tttctacgac cacggcgaag aaatctgggg cggcattccc
     9481 ggcagccccg aagaagacgg cgaccgctgg atcgaaattt ggaactgcgt atttatgcag
     9541 ttcaaccgcg acgaacaagg caatatgaat ccgctgccca agccgtccgt cgataccggt
     9601 atgggcttgg aacgtatggc cgccgtcatg cagcatgttc acagcaacta cgaaatcgac
     9661 ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg gcgcagcttt cagcatggat
     9721 gaacccagcc tgaaagtcat cgccgaccac atccgctcct gctcgttctt gattgcagac
     9781 ggcgtgttgc cttccaacga aggccgcggc tacgtattgc gccgcatcat ccgccgcgcc
     9841 gtgcgccacg gttacaaact gggtcaaagc aaaccgttct tccacaaact ggtggccgat
     9901 ttggtcaaag agatgggcga tgcctaccct gaattgaaag aaaaacaagt tcaaatcgaa
     9961 gaagcgttga aaaacgaaga aagccgtttt gcccaaacgc tggaaaccgg tatggctttg
    10021 ttggaaaacg cgttggctaa aggcggcaaa acactcgacg gcgaaatcat cttcaaactc
    10081 tacgatactt acggtttccc atacgatttg accgccgaca tctgccgcga acgcaatatc
    10141 gaactggacg aagcaggctt cgagcgcgaa atggaagccc aacgcgcacg cgcacgcgca
    10201 cgcgccgccc aaagcttcaa agccaacgcc caactgcctt atgacggtca agacaccgag
    10261 tttaaaggtt atagcgaacg ccaaaccgaa tccaaagtcc tcgccctcta caaagacggc
    10321 gagcaagtcg acgaattgaa cgaaggcgac agcggcgcag tcgtcatcga ctttaccccg
    10381 ttctatgcag aatccggcgg ccaagtcggc gacgtcggct atatcttctc aggcgaaaac
    10441 cgctttgaag tacgcgatac ccaaaaaatc aaagcagccg tattcggcca attcggtgta
    10501 caaacttcag gccgtctgaa agtcggcgac agcgttaccg ccaaagtgga cgacgaaatc
    10561 cgcaatgcca atatgcgcaa ccacagcgcg acccacctga tgcacaaagc cctgcgcgac
    10621 gtattgggcg aacacgtcga acaaaaaggc tctttggtta ccgccgaatc cacccgtttc
    10681 gacatttccc atccccaagc ggtaactgcc gaagaaattg ccgaagtcga acgccgcgtc
    10741 aacgaagcca ttttggcgaa cgttgccgtc aatgcagcca ttatgagcat ggaagacgcg
    10801 caaaaaaccg gcgcgatgat gcttttcggc gaaaaatacg gcgacgaagt gcgcgtactg
    10861 caaatgggcg gtttctcaac cgaattgtgc ggcggcacac acgtttcacg caccggcgac
    10921 atcggcctct tcaaaatcat cagcgaaggc ggtattgccg caggcgtgcg ccgtatcgaa
    10981 gccatcaccg gcctgaacgc actcaaatgg gcgcaagagc aagagcgttt ggtgaaagac
    11041 attattgctg aaaccaaagc ccaaaccgaa aaagacgtac tggcgaaaat ccaagcaggc
    11101 gcggcacacg ccaaagcctt ggaaaaagag ttggcaaaag ccaaagccga actcgccgtc
    11161 cacgcaggcg caaaactctt ggacgacgca aaagacttgg gcgcagccaa actcgttgct
    11221 gcccaaatcg aagccgacgc agccgccctg cgcgaaatcg ttaccgattt aaccggtaaa
    11281 tccgacaacg ccgtgattct tttagcggca gtaaacgacg gcaaagtctc cctgtgcgcc
    11341 ggcgtatcca aaccgttgac cggcaaagtg aaagcaggcg atctggttaa atttgcagcc
    11401 gaacaagtcg gcggcaaagg aggcggccgt cccgacttgg cgcaggccgg cggcacggat
    11461 gccggcaaat tgcccgaaat gctggtaagc gtggaaagct ggctttgtca gaaattaagt
    11521 taaatttaaa tcttaataag ccatttgagt cttgttcaaa tggcttatat tttggattgt
    11581 tagaatgaat actaaaacag agttacaaaa acttttagaa gaggatatat caactttaac
    11641 agaaacacta atttgtgcag atgctcttcc tccaagatat gtacgtagca ttgcaactcc
    11701 tattgtccgt cgatggttaa ttgacaaaca attaaatatt ctcgcaaaag aaataggttt
    11761 aactatagaa ttgcccatcc ttgatacaag tttggtattt gaaaaattgt caaccttaga
    11821 gaataaagtt aatttttata tggctggtgg agtttattta ggcggagaat ttattagctc
    11881 tatatatcat tcatcacaag aattttctgg tgaaccaata atctatgctg aaccaaatat
    11941 aattttatgt cctgctgaaa aatttttaac tttaaaacga gttttccata atggaaatat
    12001 ttttaatatg aatcagataa ttactttttt atccaataaa caaggaggag tacattttga
    12061 taaaaattac gataaatata aaacttggca agttgctata gaaaaagcag caaatttttt
    12121 aaaactcggt aatccatata atgaagataa attatctctt agtgaagaac atgatactat
    12181 tttagtagtt ttaccattag aaaaaggata tgaatggaat tgtcttgaga tagaagtact
    12241 atccgctgct caatctctag caaatatcta ttgcaacaaa gttcgcctca tagatggtca
    12301 tgtttggaag gaataaaaga aaaagcatag ttggtaattt ttagttacgt agggtgggct
    12361 tcagcccacc gttttttcat ttttgattgt tgattggtgg gctaaagccc accctacggt
    12421 tactaaaatc ccgtcattac ctcgcaggcg ggaatccggt ttgttcggtt tcggtttttt
    12481 agggacagtg ccgcaacatt aaatttccag attcccactt tcgcacaaat aacagcgcaa
    12541 atagggcaaa gataaggata tgggtttttt tctttgaatt tactatacaa ttcgcccgtc
    12601 cccgttatag aaacccaagc cgatgaaaac cctgctccaa ctccaaaccg ccgcacaaaa
    12661 cttcgccgcc tactacaaag accaaaccga cgaacgccgc gagaaagaca ccttctggaa
    12721 cgaatttttc gccattttcg gcatcgaccg caaaaacgtc gcccacttcg aataccccgt
    12781 caaagaccct gccgacaaca cccaattcgt cgatatattt tgggagggca tcttccttgc
    12841 cgaacacaaa tccgccaaca aaaacctgac caaggccaaa gagcaggcgg agcgttattt
    12901 gcaggaaatc gggcgcacca agccctctgc gctgcccgaa tattacgccg tcagcgattt
    12961 tgcccatttc cacctttatc gccgcgtacc tgaagaaggc gtggaaaacc aatggcagtt
    13021 ccctttggaa gcattgcccg aatacatcac gcgcggcgtt ttcgacttca tgttcggcat
    13081 cgaagccaaa gtccgccaaa ttcaagaaga agccgatatt caagcggcgg cggccatcgg
    13141 caggctgcac gacgcgctca aagaagaagg catttacgaa gaacacgagc tgcgcctctt
    13201 catcacgcgc ctgcttttcc tcttttttgc cgacgacagc gccgttttcc agcgcaacta
    13261 tctctttcaa gactttttag aaagctgcaa agaaaccgac acgctcggcg acaagctcaa
    13321 ccaactcttt gaattcctca acacacccga ccaaaagcgc agcaagaccc aaagcgaaaa
    13381 atttaaaggt ttcgaatacg tcaacggcgg cctgtttaaa gaacgcctgc gcaccttcga
    13441 cttcaccgcc aagcagcacc gcgccttaat cgactgcggc aatttcgact ggcgcaa
//
LOCUS       DAXIHS010000055        13213 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXIHS010000055 DAXIHS010000000
VERSION     DAXIHS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.055"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..1722
                     /locus_tag="ACFOF2_001399"
     CDS             <1..1722
                     /locus_tag="ACFOF2_001399"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC7107699.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQ
                     AEKKDGE"
     gene            1712..4492
                     /locus_tag="ACFOF2_001400"
     CDS             1712..4492
                     /locus_tag="ACFOF2_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC7107700.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNENFKNLLIWANKKIPNPNAKSNNADSLKANPQTLSFQIHGNQLLQETQ
                     FTADKNDEKVKQLGIQNNFSQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(4636..5808)
                     /locus_tag="ACFOF2_001401"
     CDS             complement(4636..5808)
                     /locus_tag="ACFOF2_001401"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC7107701.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6099..6545
                     /gene="iscR"
                     /locus_tag="ACFOF2_001402"
     CDS             6099..6545
                     /gene="iscR"
                     /locus_tag="ACFOF2_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC7107702.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6574..7788
                     /locus_tag="ACFOF2_001403"
     CDS             6574..7788
                     /locus_tag="ACFOF2_001403"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC7107703.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7855..7983
                     /locus_tag="ACFOF2_001404"
     CDS             7855..7983
                     /locus_tag="ACFOF2_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107704.1"
                     /translation="MNEQDLDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            8049..8435
                     /gene="iscU"
                     /locus_tag="ACFOF2_001405"
     CDS             8049..8435
                     /gene="iscU"
                     /locus_tag="ACFOF2_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC7107705.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8522..8842
                     /gene="iscA"
                     /locus_tag="ACFOF2_001406"
     CDS             8522..8842
                     /gene="iscA"
                     /locus_tag="ACFOF2_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC7107706.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8802..9023)
                     /locus_tag="ACFOF2_001407"
     CDS             complement(8802..9023)
                     /locus_tag="ACFOF2_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC7107707.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            9105..9605
                     /gene="hscB"
                     /locus_tag="ACFOF2_001408"
     CDS             9105..9605
                     /gene="hscB"
                     /locus_tag="ACFOF2_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC7107708.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9703..12453
                     /gene="gyrA"
                     /locus_tag="ACFOF2_001409"
     CDS             9703..12453
                     /gene="gyrA"
                     /locus_tag="ACFOF2_001409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC7107709.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            12610..13013
                     /locus_tag="ACFOF2_001410"
                     /pseudo
     CDS             12610..13013
                     /locus_tag="ACFOF2_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13093..13213
                     /locus_tag="ACFOF2_001411"
                     /pseudo
     CDS             13093..13213
                     /locus_tag="ACFOF2_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         4073 a         3207 c         2869 g         3064 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggcggcg agtttgtcta tgccgaactt
     1381 gctccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatcct tggcattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt ggatttaaat caaatgtatg tttcattatc cgaaatggat
     1621 gatgagcaat ttgcaggttg cttaaatgat gacgataaag cgttaagccg tgaattctat
     1681 caatcagcaa aaaatcaagc ggagaaaaaa gatggcgaat aataaaacgt tatttgattg
     1741 ggtggaagac cgcaaatcaa cgctggaaga aatagaacag acagattttt tcgcgctgcc
     1801 tgaatttgta gaacgcaatt taaaatatcc gttttttgaa tggcaaaaat cggctttaga
     1861 aaactttgtg atttttgacc gcgcttcaaa attaaaggat ttccctgaca tcaaaaaccg
     1921 ccctacccat ttgctgttca atatggcaac aggtgctggc aaaacgatga tgatggcggc
     1981 gttgattttg tattattttg aaaaaggtta tcggcatttt ttgtttttcg tcaatcaaaa
     2041 taatatcgtg gataaaacgg aaaataattt tattgacccg gcgcactcaa aatttttatt
     2101 taccgagaag attttgcaag gcgaccaagt tattcccatt cacaaagtag aaacgtttag
     2161 cccacattca gacggcattg aaattaaatt taccagtatt caaaaactgt ataacgatat
     2221 ccacaccgaa cgggaaaacc aaaccacatt ggcagatttg cacgaattga accttgtgat
     2281 gctgggcgat gaagcgcacc atttaaacgc acaaaccaaa ggcaaaaaac aaggcgagtt
     2341 ggatttggaa gtggaattaa aagccaatgc aggcgatgcc gaatttgaac gcaaaggctg
     2401 ggagcatatg gttttggaat tgttgctcaa taaaaatggc aatcccggcc aaaatgtgct
     2461 gttggaattt accgccacgc cacctgaaaa agctgacgtg caacaaaaat acgctgataa
     2521 aatcatcaca aaatttggtt taaaagaatt tttgcaaaaa ggttatacca aagaaatcaa
     2581 tttggtatcc agtacactga gcaagaaaga gcgggtgttg cacgctttat tgtttgcttg
     2641 gtatcgccat caaatcgctt tgaaatatgg cattgccaat ttcaagcctg tgatgttgtt
     2701 tagaagtaag acggttgatg aatcaaaagc ggattatttg gcatttttaa attgggtaga
     2761 aaatgtgcaa gcagatgatt tttcgttttt aaagacattt tcaatgagct taaacgatag
     2821 cgataacgcc aacgaacaag gcaaaacccg caccgaacaa gccctaaaat ttatgcagga
     2881 aaatggcttt gagtttgcac atttagcaaa ttggataaaa caaaattatc aaaaacacaa
     2941 tatcattatt accaactccg aaaccaacaa aagcaaaacc gaaaaaaccg acagcgaaac
     3001 ggaaaaattg ctaaacaatc tggaagcggt tgataatccg attcgtgcta tttttacggt
     3061 gggtagatta accgaaggtt gggacgtttt aaatttgttt gatattgtgc gtttgtatga
     3121 agggcaaaac ggcggcggtt caaataaaaa atcatgcaaa acggcaaata caacggtatc
     3181 agaaaaacag ttgattggtc gaggcgtgcg ttattttcca ttttcgtttg aagataaaca
     3241 gccgaataag cgtaaatttg acaacgacat gcaacacgaa ttgcgtattt tggaagaatt
     3301 gttttattac acgcacgatg agcaatcccg ctatattaca gaactgaaaa acgagttacg
     3361 aaaagacggt tatttgcctg aaaaagacga tgataaggta ttggcaacat ttaaactcaa
     3421 atctgaattt gcagataatg aaaattttaa aaacttattg atttgggcga ataaaaaaat
     3481 ccccaatccc aatgctaaaa gtaataatgc agacagccta aaagccaatc cgcaaacact
     3541 ttcattccaa attcacggca atcaactgtt gcaggaaacg caatttacag ccgataaaaa
     3601 tgatgaaaag gtcaagcaac ttggcataca aaataatttt tctcaaacca taaaaatgag
     3661 tgaaatggaa cggcacatct tcaataaagc attacatatc aaagggaaaa atagtcaatc
     3721 tttattccat ttcaaccgtt tgcaaagcaa actcaacatt caaaatcgca atgaattgca
     3781 aaataacttg ttgaaagact ggcaaattga atttttagga ttagagcaag ataaacaggt
     3841 tcgcccggat gataaacttg caggctgcct gaaaatcttg gaaatggttg aaaagcattt
     3901 gaatgaaagt gataagccat ttatcggtac aaaagaattt acgcctaaaa cattatggga
     3961 aattttcggc acgccaaaac aaaaatgggt caaaaaagat gatataaaaa ctgctattgc
     4021 catgcaaaat gattggtatg tgatggataa ttttgccgga acgggtttgg aagaagcgtt
     4081 aattcaattt atttcagcgc gtttgggcga tttgaaatct caatatgaca ttcatttaat
     4141 ccgtaacgaa gaagtgttta aattgaataa ctttgccaat ggcgaaggat ttatgcccga
     4201 ctttatttta ttgctgaaag ataaacaaaa atcttcttcc aatggcgtga atgacttttt
     4261 gcattaccaa attttcattg agcccaaagg tgagcattta gtggaaactg atggatggaa
     4321 agaagaattt ttagaagcca tcacagtgga atatgggatg gataagatcc tgcaaaaaga
     4381 tacaccgcat tatcgtttga tcggtttacc gttttttact gatcatcaga aaaatggaca
     4441 atttacggag tcattccctt taggtgcggc atcgcttgaa aaaatgaagt agtgcattgc
     4501 aggcagcccc gtttgacaaa acttccttta caaaagggcg ttttgtcaga tatttaatca
     4561 acacattatt aaaatacagc caaattttaa tgccgtctga accctgtgtt cagacggcat
     4621 cgtatttttc agtatctaaa ccgtttccct accccaatcc ttacccctca aaatcgaggc
     4681 atcgacatct tgaatatcgc ggtgtcccgt aaacgccata gatatatcca tttctttata
     4741 caggatttcc agcgcacggg ttacgccttc ttcgccatac gcacccaagc catacagaaa
     4801 cgcccgacct atcattgtac ctttcgcacc caaagcccac gccttcaaaa tatcctgacc
     4861 gctgcggatg ccgctgtcca tccaaacttc gatgtcgctg ccgactgcac taacgatgtc
     4921 gggcaaggct ttgatggcag acacggtatc gtcgagctgt cggccgccgt ggttggaaac
     4981 aatcaatgcg tccgcgccgc ttttcgctgc tttttccgcg tcttcgggtt ccataatgcc
     5041 tttgataatc agcttgccgc cccacaaatc tttaatgcgc gccacatcgt cccagctcag
     5101 gcgcgggtcg aattgttcgg aagtccatga agacagcgaa gacaaatcgc cgacgttctt
     5161 cgcgtgtccg acgatattgc ggaacgtgcg gcgttccgtg ttcagcattt tcatgcacca
     5221 ttccggtttg gtcgccagat tgattaaatt ggcgatggtc ggtttcggcg gcgcggacag
     5281 gccgtttttg atgtctttgt ggcgttgacc caaaacctgc aaatcggcgg tcaataccaa
     5341 tgccgaacat ttggcatcct tcgcgcgctt aatcaggttt tccataaact cgcggtcgcg
     5401 catcacataa agctgaaacc aaaatggtgc ggaagtgttc tcggcaacgt cttcaatcga
     5461 gcagatagac atcgtggaca gcgtaaacgg aatgccgaac ttctccgccg cccgcgccgc
     5521 caaaatttca ccgtcggcgt gtgccatacc cgtgaaaccc gtcggcgcaa tcgccaccgg
     5581 cattttcaca tcctgcccga tcattttggt ttccaggctt cggccttcca tattgaccaa
     5641 taccttttgt cggaagcgga tatctttgaa atccgaagtg ttttcacggt aggtagtttc
     5701 tgtccacgaa cccgaatcga tgtaatcgta aaacatacgc ggcattttgc gcttggcaac
     5761 gcggcgcaag tcttcgatgc aggtcatttt gctcaaatca cgtttcattt gtcgccccct
     5821 gaatacctga ataactttat atgaaatcga taatgtatat caatattgat tataaggcaa
     5881 atcatttcaa catttgccgc atccgccgca gctccctact ttaagcgaca taaggtttaa
     5941 aattcaaaaa taacaaatta aaatcaaaat attaaaaatc aatcaatcta tcgatttaaa
     6001 cagccaatca cacaatccgc ccccatactt gactgaaaca ctcagatatt ggacaattcc
     6061 acccactaat aaaaaaaccg acatgggcaa ccaccaccat gagactgacc accaaagggc
     6121 gtttcgccgt taccgctatg ctggatttgg cgatgaacgc gcaaaccggc gccgtcaaac
     6181 tcagtgccat cagcgaacgc caaaacatat ccctctccta tctcgagcaa ttgttcggca
     6241 aactccgccg cgccggactt gttgaaagcc tgcgcgggcc cggcggcggc tacatcctcg
     6301 ccgcaccggc ggcacgcatc aacatcgccc aaatcatcgc cgccgccgaa gaccggctgg
     6361 acgcaaccca atgcggcagc aaagccaact gccaccacgg cgcgccctgc ctgacgcacg
     6421 atctttggga gaatttaaac aaaaccatca acgactacct cggcagcgtt accctgcaaa
     6481 gcatcatcga acagaaaaac aacggcgacg gcagccgcgt cgtccaattt acacacatcc
     6541 attaaataac acccgaaaaa gaaagagcaa accatgaccg tcaaaacccc cgtttacctc
     6601 gactacgccg ccaccacccc cgttgacaaa cgcgttgccg aaaaaatgat tccctatctg
     6661 accgaaacct tcggcaaccc agcctccaac agccacagct tcggctggga agcagaagaa
     6721 gctgtagaaa aagcacgtgc agacattgcc gccctgatta acgccgactc taaagaaatc
     6781 gttttcacca gcggcgcaac cgagtccaac aacctcgcta tcaaaggcgc ggcgcacttc
     6841 tacaaatcta aaggtaatca cctcatcact gtaaaaaccg aacacaaagc cgtactcgac
     6901 accatgcgcg aactcgaacg ccaaggttac gaagtaactt atctggacgt acaagaaaac
     6961 ggtttggttg atttagacgt actgaaagcc gccatccgcg aagacaccat cctcgtttcc
     7021 gtaatgtggg taaacaacga aatcggcgtg gttcaagata ttcctgccat cggcgaaatc
     7081 tgccgcgaac gcaaaatcat tttccacgtt gacgcagcac aagcatgcgg caaagtgcct
     7141 gttgatgttg aagccgcaaa agttgatttg ctgtctatgt ccggccacaa agtatacggc
     7201 cctaaaggca tcggcgcgct gtacgtccgc cgcaaacccc gcgtccgcct cgaagcccaa
     7261 atgcacggcg gcggccacga acgcggcttc cgcagcggca cattgccgac ccaccaaatc
     7321 gtcggtatgg gcgaagcctt ccgcatcgcc aaagaagaac tcgaacaaga tatggcgcac
     7381 taccgcaaac tgcgcgacat cttcctcaaa ggcatcgaag gcatcgaaga agtctatgtc
     7441 aacggcgacc tcgaacaccg cgccccgaac aacctgaacg tcagcttcaa cttcgtcgaa
     7501 ggcgaaagcc tgattatggc ggtgaaagaa ctcgccgtat ccagcggctc cgcctgtact
     7561 tctgccagtc tcgaacccag ctacgtcctg cgcgcgctcg gccgcaacga tgaactggcg
     7621 cactcatccc tgcgcatcac cttcggtcgc atgaccactg aagaagaagt gcaatttgcc
     7681 gccgaactga ttaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgtt gtgggaaatg
     7741 ttcaaagacg ggattgattt gaactcgatt gaatgggcgg cgcattaaag cgtaccaaca
     7801 tgccatccga accttcagac ggcattccaa aaacaaagca atcaagagaa aaatatgaac
     7861 gaacaagatt tagatttgga caacctcgac aacctgcttg acgatttcga cggcgttacc
     7921 gtggaaggcg gcgtcgattc ggaaaacgac gacggctgtg aaggcggagc gtgcaaaatc
     7981 taactcaggc cgtctgaaaa ccggcaaaca aaccacttaa atcatcaaaa cattaaggaa
     8041 accacatcat ggcatacagc gataaagtaa tcgaccacta cgaaaatccc cgcaacgtcg
     8101 gtactttcga caaaaacgac gaatctgtcg gcaccggcat ggtcggcgca ccggcctgcg
     8161 gcgacgtgat gcgcctgcaa atcaaagtga acgatgaagg catcatcgaa gatgcgaaat
     8221 tcaaaactta cggctgcggt tccgccatcg cttcgtccag cctgattacc gagtgggtta
     8281 aaggcaaaag cctggatgac gcgctggcaa tcaaaaacag cgaaatcgcc gaagagttgg
     8341 aattgccgcc ggtaaaaatc cactgctcca tcttggctga agatgcggta aaagcggccg
     8401 ttgccgacta ccgcaaacgt caggaaaaca gataaagccc ttcagacggc atcgtcccgc
     8461 aatgccgtcc gaaccgcccg ccgcttcggg ttcgtccgag gcggtacaac aaggaagaaa
     8521 tatgattacc cttaccgaga atgccgcaaa acacatcaat gactatctcg ccaaacgcgg
     8581 caaaggcttg ggcgtacgct tgggtgtgaa aaccagcggc tgctcgggga tggcgtacaa
     8641 ccttgaattt gtcgatgaag ccgatggcga cgacctgatt ttcgaaggac acggcgcacg
     8701 catttatatc gacccgaaaa gcctggttta tctggacggc acacaagtcg attacaccaa
     8761 agaaggtttg caggaaggtt tcaaatttga aaaccccaat gtcaaagact cctgcggctg
     8821 cggcgaaagc ttccatgttt aagacataaa aatggcggga ccgtatcaaa accgtcccgc
     8881 cattttttcg cttcctgcct gttgtagctg cctttgcctt ttcttttccg ttccaccttg
     8941 tgtcggaaca aatcagattt cactaaggct tttaaagcat tgtcgcgtat tttgccttta
     9001 ttgtgctgca ctttgccgcc catattcagt cctttcgttt aagaagcggc agattataag
     9061 gcaaaaacag ttttctgcca aaatcttaca tttatcatcc tactatgtcc caatatttca
     9121 ccctcttccg gattgaaccc gctttcgata tcgacaccga aaacttggaa caaacctacc
     9181 gcgccttggc cgcccgtttc catcccgata aattcgcttc agcttccgcc tttgagcaaa
     9241 agcaggcagt gatgatgtct tccaccatca acgatgccta ccgcaccttg aaaaacccca
     9301 tcgaccgcgc cgcctacctg ctgaaaaaat cgggcatcga tgccgacgcg ccggagcata
     9361 ccgctttcgc ccccgaattc cttatgcagc aaatggaatg gcgcgaaacg ctgatggagg
     9421 cacgggcagg caacgacctt gaatccttga aaaatctcga caacgaaatc cgcgacgagc
     9481 aagaaaaact gttctgcggt ctgaaacagt cgtttgcccg acaagattac gacacagccg
     9541 cacaacaagt ccgccaaggc aggtttctcg acaaactccg caacgaaatt tcctcggcat
     9601 tataatccgc accgtgtttc agacggcgta accgccgcac cgttccgcgt caaaatatgc
     9661 taaaataagc aacaattttt tgccatacga aacattgaaa ccatgaccga cgcaaccatc
     9721 cgccacgacc acaaattcgc cctcgaaacc ctgccggtaa gccttgaaga cgaaatgcgc
     9781 aaaagctatc tcgactacgc catgagcgtc attgtcgggc gcgcgctgcc ggacgttcgc
     9841 gacggtctca agccggtaca ccgccgcgta ctgtacgcga tgcacgagct gaaaaacaac
     9901 tggaatgccg cctacaaaaa atcggcgcgc attgtcggcg acgtcatcgg taaataccac
     9961 ccccacggcg ataccgccgt atacgacacc atcgtccgta tggcgcaaaa tttcgctatg
    10021 cgttatgtgc tgatagacgg acagggcaac ttcggatcgg tggacgggct tgccgccgca
    10081 gccatgcgct acaccgaaat ccgcatggcg aaaatttccc acgaaatgct ggcagacatt
    10141 gaggaagaaa ccgtcaattt cggcccgaac tacgacggta gcgaacacga gccgcttgta
    10201 ctgccgaccc gtttccccac actgctcgtc aacggctcgt ccggcatcgc cgtcggcatg
    10261 gcgaccaata tcccgccgca caacctttcc gataccgtca atgcctgcct gcgcctgctc
    10321 gatgcacccg acaccgaaat cgacgaactg atcgacatta tccaagcccc cgacttcccg
    10381 accggggcaa ccatctacgg cttgagcggc gtgcgcgaag gctataaaac aggccgcggc
    10441 cgcgtcgtta tgcgcggtaa gacccatatc gaacccatag gcagaaacgg cgaacgcgaa
    10501 gccatcgtta tcgacgaaat cccctatcag gtcaacaaag ccaagctggt cgagaaaatc
    10561 ggcgatttgg ttcgagaaaa aacactggaa ggcatttccg agctccgcga cgaatccgac
    10621 aaatccggta tgcgcgtcgt tatcgagctg aaacgcaacg aaaatgccga agtcgtctta
    10681 aaccaactct acaaactgac tccgctgcaa gacagtttcg gcatcaatat ggtggttttg
    10741 gtcgacggac aaccgcgcct gttgaacctg aaacagattc tctccgaatt cctgcgccac
    10801 cgccgcgaag tcgttacccg acgtacgctt ttccggctga agaaggcacg ccatgaaggg
    10861 catatcgccg aaggcaaagc ggtcgcactg tccaatatcg atgaaatcat caagctcatc
    10921 aaagaatcgc ccaacgcagc cgaggccaaa gacaaactgc ttgcgcaccc ttggcgcagc
    10981 agcctcgttg aagaaatgct gacgcgttcc ggtctggatt tggaaatgat gcgtccggaa
    11041 ggattggctg caaacatcgg cttgaaagag caaggttatt acctgagcga gattcaggca
    11101 gatgctattt tacgcatgag cctgcgaaac ctgaccggcc tcgatcaaga agaaattgtc
    11161 gaaagctaca aaaacctgat gggtaaaatc atcgactttg tggatatcct ctccaaaccc
    11221 gaacgcatta cccaaatcat ccgcgacgaa ctggaagaaa tcaaaaccaa ctatggcgac
    11281 gaacgccgca gcgaaatcaa cccgttcggt ggcgacattg ccgatgaaga cctgattccg
    11341 caacgcgaaa tggtcgttac cctgacacat ggcggctata tcaaaaccca gccgaccacc
    11401 gactatcagg cgcagcgtcg cggcgggcgc ggcaaacagg cggctgccac caaagacgaa
    11461 gactttatcg aaaccctgtt tgttgccaac acgcatgatt atttgatgtg ctttaccaat
    11521 ttgggcaagt gtcattggat taaggtttac aaactgcccg aaggcggacg caacagccgc
    11581 ggccgtccga ttaacaacgt catccagttg gaagaaggcg aaaaagtcag cgcgattctg
    11641 gcagtacgcg agttccccga agaccaatac gtcttcttcg ccaccgcgca gggaatggtg
    11701 aaaaaagtcc aactttccgc ctttaaaaac gtccgcgccc aaggcattaa agccatcgcg
    11761 ctcaaagaag gcgactacct cgtcggcgct gcgcaaacag gcggtgcgga cgacatcatg
    11821 ctgttctcca acttaggtaa agccatccgc ttcaacgaat actgggaaaa atccggcaac
    11881 gacgaagcgg aagatgccga catcgaaacc gaaatttcag acggcatcga agatgaaacc
    11941 gccgacagcg aaaacgcact gccgagcggc aaacacggtg ttcgcccgtc cggtcgcggc
    12001 agcggcggtt tgcgcggtat gcgcctgcct gccgacggca aaatcgtcag cctgattacc
    12061 ttcgcccctg aaaccgaaga aagcggtttg caagttttaa ccgccaccgc caacggatac
    12121 ggaaaacgca ccccgattgc cgattacagc cgcaaaaaca aaggcgggca aggcaatatt
    12181 gccattaaca ccggcgagcg aaacggcgat ttggtcgccg caaccttggt cggcgaaacc
    12241 gacgatttga tgctgattac cagcggcggc gtacttatcc gcaccaaagt cgaacaaatc
    12301 cgcgaaaccg gccgcgccgc agcaggcgtg aaactgatta acttggacga aggcgaaacc
    12361 ttggtatcgc tggaacgtgt tgccgaagac gaatccgaac tctccgacgc ttctgtaatt
    12421 tccaatgtaa ccgaaccgga agtcgagaac tgaaaatcat ctcccgatgc cgtctgaaga
    12481 ttcagacggc atttatttta tccctcatcc gtcatccagc ttctcacaat atagcggatt
    12541 atagtcaatt aaaaacaagg ggctgtccta gataactagg gaaattcaaa ttaagttaga
    12601 gttgccccta tgagaaaaag tcgtctaagc cggtataaac aaataaactc attgaactgt
    12661 ttgtcgcagg tgtaactgca agaacgacag cagagttagt aggcgttaat aaaagtaccg
    12721 cagcctatta ttttcatcgt ttacgattac ttatttatca aaacagtccg catttggaaa
    12781 tgtttgatgg cgaagtagaa gcagatgaaa gttattttgc tgaacgacaa aaccatatca
    12841 atggaattga gaacttttgg aaccgggcaa aacgtcattt acgcaagttt gacggcattc
    12901 ccaaagcgca ttttgagctg tatttaaagg ggtacgaacg gcgttttaac aacagtgaga
    12961 taaaagttca aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttatcta
    13021 ggacagcccc aaaaacaaaa tagtacaata ttcaactttg aaggtctaac catggcatac
    13081 tctgcggact taagaaacaa agctttaaac tatagtggat taaatttaaa tcaggacaag
    13141 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacacc gtactggttt
    13201 aaatttaatc cac
//
LOCUS       DAXIHS010000056        13092 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXIHS010000056 DAXIHS010000000
VERSION     DAXIHS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.056"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..415)
                     /locus_tag="ACFOF2_001412"
     CDS             complement(<1..415)
                     /locus_tag="ACFOF2_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC7107710.1"
                     /translation="MKTVIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNS"
     gene            complement(632..1555)
                     /gene="truB"
                     /locus_tag="ACFOF2_001413"
     CDS             complement(632..1555)
                     /gene="truB"
                     /locus_tag="ACFOF2_001413"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC7107711.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYT
                     EIGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1588..1959)
                     /gene="rbfA"
                     /locus_tag="ACFOF2_001414"
     CDS             complement(1588..1959)
                     /gene="rbfA"
                     /locus_tag="ACFOF2_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC7107712.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            2149..3393
                     /gene="clpX"
                     /locus_tag="ACFOF2_001415"
     CDS             2149..3393
                     /gene="clpX"
                     /locus_tag="ACFOF2_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC7107713.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(3458..4651)
                     /locus_tag="ACFOF2_001416"
     CDS             complement(3458..4651)
                     /locus_tag="ACFOF2_001416"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HFC7107714.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            5055..5555
                     /locus_tag="ACFOF2_001417"
     CDS             5055..5555
                     /locus_tag="ACFOF2_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107715.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDARDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5640..6194
                     /locus_tag="ACFOF2_001418"
     CDS             5640..6194
                     /locus_tag="ACFOF2_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107716.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            6208..6408
                     /locus_tag="ACFOF2_001419"
     CDS             6208..6408
                     /locus_tag="ACFOF2_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107717.1"
                     /translation="MPSEGLSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(6683..8071)
                     /locus_tag="ACFOF2_001420"
     CDS             complement(6683..8071)
                     /locus_tag="ACFOF2_001420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC7107718.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            8400..9347
                     /locus_tag="ACFOF2_001421"
     CDS             8400..9347
                     /locus_tag="ACFOF2_001421"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7107719.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(9400..9732)
                     /gene="trxA"
                     /locus_tag="ACFOF2_001422"
     CDS             complement(9400..9732)
                     /gene="trxA"
                     /locus_tag="ACFOF2_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC7107720.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            10034..10507
                     /gene="dnrN"
                     /locus_tag="ACFOF2_001423"
     CDS             10034..10507
                     /gene="dnrN"
                     /locus_tag="ACFOF2_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC7107721.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(10577..11398)
                     /gene="nadE"
                     /locus_tag="ACFOF2_001424"
     CDS             complement(10577..11398)
                     /gene="nadE"
                     /locus_tag="ACFOF2_001424"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC7107722.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            11595..12950
                     /gene="xseA"
                     /locus_tag="ACFOF2_001425"
     CDS             11595..12950
                     /gene="xseA"
                     /locus_tag="ACFOF2_001425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC7107723.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         3060 a         3443 c         3493 g         3096 t
ORIGIN      
        1 ggctattact gcgtttaaaa taaatctctt cgccttcttc ggtgctttgt ccgccattca
       61 atacgcaatc tttaaacgga aaatccaaca caatatccga attgtctttc aaaaagcggt
      121 tgccgtccgt caaaccaatg cggttggcgt attttgtgta ggaattattg acgctgtgtt
      181 tgtccaagaa aaaacgtaaa tcctgcaatt taaacaccaa aacaccattc acttccacaa
      241 agaaatggcg ttttaactca tcattattca ataacaaacc gataatggtc gggtcggttt
      301 tttccaccaa atccaacaga atgtttttgg ctaaaatggt tttttcttcg ttcgcccata
      361 ctttttcgtg tgatagcagg gcttgggtta attcggtttg aatgactgtc ttcatcgtta
      421 taaatcctca tcagtcattt taatgtgcca ttcttgtgta acggcaaatg gctttgcaaa
      481 taaattctaa taaaatcaat cctttgatgt cttttctgtt tcggggggcg ttcgcaaagt
      541 tgcgataagc ggggagggtg tccgagtgtg tcgggatttt gcggaaatat caacacatta
      601 ttcaaacaca gcccgtattt ttaaccgtcg ttcaggcggc ggatgccgcc gtgttcatca
      661 ggcgcaaggc tttcagacgg catatttcct tttgatattc tgccaaaccg acaaattctc
      721 caatttcagt gtaaacgcgt accggcgtgc cggaaggtag gtcttcctcg aaacgcggac
      781 gttgtccgca gcggagcata tggacggcat aatcgtttaa aacggtttgg ggaaagtgtg
      841 aaaccaatac gtcgcaggat agcagcaagc tgtcgcgttc tgtttcgttc aaatttgcca
      901 aggcctcaag cgtgtggctt tgggcgatgg taaagccggc ggtttcggtg cggcgcaagg
      961 cggtcaggtg ggcgaacgtg ccgatgtgtt tggcgatgtc ttcgctgagg gtgcggatgt
     1021 aggtgccttt gctgcaacgt acgtcgatga cggctttggg cgcgtcaaat tcggcaatat
     1081 cgatggcgta aacggtaatg tagcgcgctt tgcgttcgat gacgatgcct ttgcgggcgt
     1141 attcgtacag cggtttgcct tcgtgcttga gcgcggaaaa cattggcggc acttggcgga
     1201 tgtcgcccgt taatgctttg caggccgtct gaaattcggc taaggaaata tcggcacggg
     1261 cggtggcaac gatttcgcct tcggcatcac ccgtgctgct ggcttcgccg agtttcagcg
     1321 tggcggtgta ggctttgtcg gcatccagca ggtattgggc gaacttggtc gcttcgccga
     1381 agcagacggg caaaagtccg gttgccaaag gatcgagcac gccggtatgt ccggcttttt
     1441 cggcacggaa caaacgccgc gctttttgca gcgcggtgtt gctggaaagg ccttcgggtt
     1501 tgtccaagag caaaacaccg ttgacggcac gtttttgggg tttgcctgta ttcatggttc
     1561 gatgttcgga cggcatgggc atatccgtca gtcttcaacc ggtttttccg ccgctacttg
     1621 gtcgataagg gcggacaggt tcataccgcg ttccaaggat tcgtcgtatt tgaaatgcag
     1681 ctcggggatt ttgaacagct tgatgcgttt ggccaattcg ctgcggaggt gtccgcgcgc
     1741 gtgttccagc acttcttccg taatttcgcg cgcgtcttgg tttaaaatgg tgtagaacac
     1801 ggtggcgtgg ctgtaatcgc gggtaacttc gacttcgttg acggtaatga agccggcgcg
     1861 cgggtctttc agtccggtac ggacgagttc ggcaagctcg cgcataattt gttctttgac
     1921 acggtcttgg cgggcatagc cgcgttgggg ttttctcatg aatatcctca gatttcagat
     1981 ttggtttcag acggcatttt tgccgccggg gcggttgcga aagggcgtat tatagccgaa
     2041 tcggcgggat tttaaggtaa tacattgata agaacggata tagttattat acttggcaac
     2101 ccgaacggcg cgtgccgttt ctgtaacgca tacattgtaa agaaagccat gtccaacgaa
     2161 aaccgtacct gttccttttg cggaaaatcc aaatcacacg tcaaacattt aattgagggc
     2221 gaaaacgcct ttatctgtga cgaatgcgtc tcaaactgta ttgaaatatt gcatgaagac
     2281 ggaaatgacg gcacgccttc ggaaagtgcc ggaggggagc cggaagaatc cggcaagctc
     2341 cccacgcccg ccgaaatcgt cgccaacctc aacgaccacg ttatcggtca ggaacaagcc
     2401 aagaaagcct tggcggtgtc ggtgtacaac cattacaaac gcctgcgcca cccgaaagcc
     2461 ggagctaatg tcgaattgtc gaaatccaac atcctgctta tcggcccgac cggatcgggt
     2521 aaaaccctgc tggcgcaatc tttggcgcgc aaactggatg tgccgttcgt gatggcagat
     2581 gcaaccacgc tgaccgaagc aggttatgtc ggcgaagatg tcgagcaaat catcaccaaa
     2641 ctcttgggca aatgtgattt cgatgtcgaa aaagcccaac gcggcatcgt ctatatcgac
     2701 gaaatcgata agatttcacg caaaagcgac aatccgtcca tcacgcgtga cgtgtccggc
     2761 gaaggcgtgc aacaagcctt gctgaaactg attgaaggta cggtggccag cgttccgccc
     2821 caaggcggcc gcaagcatcc gaatcaggaa tttatcaacg tcgataccac caacatcctg
     2881 tttatctgcg gcggcgcgtt tgcaggcttg gaaaaagtca tccgccagcg tactgaaaaa
     2941 ggcggtatcg gtttcggtgc gtccgttcac agcaaggacg aaaatgccga cattaccaag
     3001 ctgttcggca tcgtcgaacc ggaagattta atcaaattcg gtctgattcc ggaattaatc
     3061 ggacgtttgc ctgtgattgc aacgttagaa gaactggatg aggacgcgct gattaatatt
     3121 ttgaccgagc cgaaaaacgc tttggtcaag cagtatcaag ccttgttcgg tatggaaaat
     3181 gtcgagttgg aatttgaaga aggcgcattg cgttccattg cgtggcaggc aatggagcgc
     3241 aaaaccggcg cgcgcggcct gcgttccatc gtcgaacgct gcctgctcga tacgatgtac
     3301 cgcctgcctg atttgcaagg cttgaaaaaa gtggtggtcg gtaaggcagt catcgaagag
     3361 ggcaaggaac cggaattggt gttcgagtcc tgatggcaaa caaaaccgct tcggaacgtt
     3421 gaaccacgtt tccgaagcgg ttttttgttt ttgatgccta ggggcggttt gcggcggcaa
     3481 attccgtcag cacgtgttcc aaacgccgca aaccttccgc cgtatcctca tcgtccaaca
     3541 gcagcgaagg cgcgaaacgc aatacgtccg cacccgcaac caaaatcatc acgccgtgtt
     3601 tcaaggcggc ggcggttatt tcggatgcgc gtccgcgata aggcgcgtcc aacacgcagc
     3661 cgagcagcag ccccatgccg cgaacttgct taaacacgcc cgtttgttct cccaaagacc
     3721 gcaatgccgt ctgaagtttc tgcccctgac tgcagacgtt gtgcaaggtt tccggcgcgt
     3781 tgatgatgtc gaaggcgcgg ctgccgaccg cacacgccat cgggttgccg ccgaaagtcg
     3841 agccatgcgt gcccggttgg aaggcggcgg caatcgtttc tgtcgccagc atcgcgccga
     3901 tcggaaagcc gcagcccaag gcttttgccg aactcaaaat atcgggaaca acgccgtaat
     3961 gttcataggc aaacagcctg cccgtatgcc ccatcccggt ttgaacttcg tccaaaatca
     4021 acaacgcatt gtgccggtcg cacagacggc gcgcggtttg caaatattcc gcagtggcgg
     4081 gcaggatgcc gctttcgccc tgtatcggct cgatgatgac cgcgcaggtc tgttcgccga
     4141 cggcagcttc cagcgcggca atatcgttga acggaacgtg cgtaatgcct tgcggcaggg
     4201 gtgcataatc cttgctgtat ttcggctgac cgccgacgga cacggtaaac agcgtgcgtc
     4261 cgtggaaact gttgatacag gcgacgattt cgctttttcc tccgccgaaa cggtcgcgcg
     4321 catatttcct tgccagcttc aacgccgcct cattcgcttc cgcgcccgag ttgcagaaaa
     4381 aaaccttgtc ggcaaaactg ttcttcacca atttttgcgc caattcctgc gccggacgcg
     4441 tcgtatagat attggaaatg tgccacagct tgtgcatctg cgtgtttaaa gcatcggcaa
     4501 gggcagggtg gcagtgtccc agcgcattga cggcgatgcc gcctgaaaag tcaatatatt
     4561 cacgcccttc cgtatcccaa acgcggctgc ctgaagcgcg ttcgggaatc atcggtgcaa
     4621 aggcgaaatt gggggtcaga tagttttgca taattgctcc tgattgcggt tttgctcggg
     4681 attatgcacc gtttttcaaa gcagataaag ggaaattgcc gacaatatcg ggcagcggta
     4741 aaaataaacc gaaatatttt caatcggata gatgaaaaat caaaaaaatt tcgataaaat
     4801 gcttgaggcg cggcagcgtt ttgatttaga atctccccaa tatttttatc ttcagacggc
     4861 atttcatcat gcaaggcaaa aaatttacaa ccgcttattg ctattggtac cacacagacg
     4921 tgcggtactt ttttgcgctt gcttgaaatc catcccatac gcaaaacagg ccgctcgaaa
     4981 atggggcggc ttttttgtta ccttcacaca ggtcgcccga ttcggcaaca cacgacagaa
     5041 tcaggaaaat gaccatgaat gcaatgcccc aaaccgccgc acaaaacgaa accctgcaca
     5101 tttgcagcat tattgtcgat gatgcacggg atttttcccc catgcttctc gatgcagggc
     5161 gggacgacac actcatcagc gaatcgatga ttccgcaaat ccgcactgtt gccgccctga
     5221 tagccgccga acggcacgac ttcagccgca gcagccccgc cgagtttacc gatgccgccg
     5281 atttttttgc cgcacggatt ctcgtattgg gcgtgcgacg cttccatctg gacgtatcgc
     5341 tgcttcagat tttgaaaacc gccaacagcc gcgcccgccg ctttgccgaa aaacaccgcc
     5401 tgtttttcac acccgcacaa gccgaattga gcctgaacaa acacaaaaac cgccgcctgc
     5461 tgaccgtaga aaccgaacac gaagtcgaaa acaaaggcaa cccagtcgcc aacagcctcg
     5521 catttgtccg caaattgcac accctgcccc tgtaaacatc acgatttgcc ggcagaatgc
     5581 ttgccaaaat tcccgacggc gatacaataa aaccgatttt ccacccaaac aggacacaca
     5641 tgaaaaaaat catcgcctcc gcgcttatcg caacattcgc actcgccgcc tgccaagacg
     5701 acacgcaggc gcggctcgaa cagcagcaga aacagattga agccctgcaa cagcagctcg
     5761 cacagcaggc agacgatacg gtttaccaac tgactcccga agcagtcaaa gacaccattc
     5821 ctgccgaagc acaggcaaac ggcaacaacg ggcaacccgt taccggtaaa gacgggcagc
     5881 agtatattta cgaccaatcg acaggaagct ggctgctgca aagcctggtc ggcgcggcgg
     5941 caggcgcgtt tatcggcaac gcgctggcaa acaaattcac acgggcaggc aaccaagaca
     6001 gtcccgtcgc ccgacgcgcg cgtgcagcct accatcagtc cgcacatccc aatgcgcgca
     6061 ccagcaggga tttgaacacg cgcagcctcc gtgcaaaaca acaggcggcg caggcgcagc
     6121 gttaccgccc gacaacgcgc ccgcccgcca attaccgccg ccccgctatg cgcggtttcg
     6181 gcaggaggcg gtaaacccgg cgtgccaatg ccgtctgaag ggctttcaga cggcattttt
     6241 gcatttgtta gggacattgt tatgttgccg tttgattttc agacggcatt ttgtttccaa
     6301 gcgtttgatg tcgggatggc aattctgatt tacgtgcgtt ggcagggtgg cattgtcatg
     6361 ccgccgttgg gttttcagac ggcatcatat cgaacagtca agccgtaggg cgagcattct
     6421 tgcccaccgt tgcaagggcg ggattgggtg ggcaggaatg cccaccctgc atctgaaacc
     6481 cttccgtgtc attcccgtgc aggaaggaat ctagatattt gaatttacgg aaattttcaa
     6541 tcgttgccaa agcggaaaag ggtttttatt acagcctaat cctttgggta tcaaagccgg
     6601 gcggtgttga aacattgcaa aaatgccgtc tgaaacccga aatcggtttc agacggcatt
     6661 ggtttatgcg gcaggttggg gcttagcggt ctttgtaacg gcgttcgcct ctgcctgcaa
     6721 aagaatcgcc ggctttacgt ttgcctttga agccttcgcc ggtttttttg aaaccgtctt
     6781 tcttaaagcc ttctttcttg aaaccttcgc cgcgcgtttt gccgccgaag ccttctttgc
     6841 ccggtttatg atcgccgcgc cggccgccgg atttcctatc gccccagccg cctttgcctt
     6901 ttggcttacc gcctgcggat ttgcgtttgc gggtcggttc catgccttcg atggtcagtt
     6961 cgggcagttt gcggttaatg tatttttcga ttttgtggac tttgacgtat tcgttcactt
     7021 cggcaaacgt aatcgcaata cccgtgcggc ctgcgcggcc ggtgcgcccg atgcggtgga
     7081 cgtagtcttc cgcctgtttc ggcaggtcgt agttgatgac gtgggtaatg gtcggtacgt
     7141 cgataccgcg tgcggcaacg tcggtggcaa ccaaaatttt gcagcggcct ttgcgtaaat
     7201 ccatcagcgt gcggttgcgc cagccctgcg gcatgtcgcc gtgcaggcag ttggcggcga
     7261 aacctttttc gtacaattca tccgcgatga cttcggtcat cgctttggtg gacgtgaaaa
     7321 tcacgcattg atcgatgttg gcatcacgca ggatgtggtc gagcaggcgg tttttgtgac
     7381 gcatatcgtc gcagtacagc agttgttctt cgattttgcc ttggtcgtcc acgcgttcga
     7441 cttcgatgat ttcagggtct ttggtcagtt tgcgcgccag tttgccgacc gcgccgtccc
     7501 aagtggcgga gaacaacaaa gtctgacggt cgctcggcgt tgcttccacg atggtttcga
     7561 tgtcgtcgat aaagcccatg tctaacatgc ggtcggcttc gtccaaaatc agcacttcca
     7621 aacgttcaaa atcaactttg ccgctttgca tcaggtccat cagacggccc ggcgtggcga
     7681 caatcagatc gaccggtttg ctcagggcgc gggtttggta gccgaacgat gcgccgccga
     7741 cgatgctgac ggtacggaac caacgcatat ttttggcata cgccagcgcg tttttctcga
     7801 cttgagccgc caattcgcgg gtcggggtca acaccaaagc acgcgggcct ttgcccggtt
     7861 tttcgctgcg tttggtcagt ttttgcaaag tcggtaacag aaaggcggcg gttttgccgg
     7921 agccggtttg cgccgaagcc atgatgtcgc ggccttccaa agcaaacgga atcgcctgcg
     7981 cctgaatcgg cgtcgggctt tcgtaaccct cgctgctgac ggcggacaag atgtttttat
     8041 cgaggttcaa atcggcaaat ttaatagaca tggctatcct tggaagacaa caacgcccgc
     8101 gtccgaaagg ttcagacggc ctgaagcagg gaagggtaac gatacgggga atgtgaagtt
     8161 acagggttgt cgcaagcggc ttgcttgtgg tttacatcga cgacaacctg cacagaaagg
     8221 cttggcaaat atgcaagcaa caaaagaaac gcgcccgtaa gggcggttta ggttggaagg
     8281 cgatggcttc gtatgacaag ataagcggcg gatggtatag ggtttgcccg aaacggtcaa
     8341 gcaatcgggc gttaattaca gtaaaattgc cgttttccac tttattccgc ctgaaaccaa
     8401 tgaccgacat cacaccgttt gccaaccgct tgggcaaaaa catcaaacac cttatgaaat
     8461 gggcgaaacg caacggcatc gaagcctggc gcatttacga ccgcgacatc ccccaattcc
     8521 cctttgccgc cgatgtttac ggcgatcgaa tccaccttca ggaatacgat accggctggc
     8581 tgatgcggcc cgaagaatac gaagcgtggc ttgccgaagt cttggaagcc gtcgccttcg
     8641 ttaccggttt tgcgcccgaa caaatccgcc tcaaacgccg cgaacgccaa aaaggtttgc
     8701 agcaatacga aaaaaccggc aaggcgggcg acgatttcgt catcaccgaa aacggccgca
     8761 agttttgggt caaccttgat aagtatttgg atacggggct ttttttggat catcgaaaca
     8821 cgcgcaaaaa agtcggcgaa acggcggcgg gcaaacgttt tttaaacctg ttttcctaca
     8881 ccggcagctt cactgtctat gccgccaccg gcggcgcggc atccagcgaa actgtcgatt
     8941 tgtctaacac ttatctcgat tgggcgagac gcaattttga attgaacggc atcgatacgg
     9001 aacggcataa aatcgtccgc gccgacgtgt tccaatatct tcagacggca tatggggaag
     9061 gcaggcggtt cgacctcatt gtgatggacc cgcccagttt ttccaacagc aagaaaatgt
     9121 ccgacatcct cgacatccag cgcgaccaca aaaagctgat tgacggcgcg gtgaagctgc
     9181 tcgcttcaga cggcattttg tacttttcca acaacctgcg cagcttcgtc ttagacgact
     9241 tggtatcgga acaatacgcc gtcaaagaca tttccaaaca atccgttccc gaagatttcc
     9301 gcaacaaaaa aatccatagg tgctgggaaa tccgccacaa atcttaaggg tgcgaaaaag
     9361 ccgccggaat cttccggcgg ctttttcgat tgcgctttat caggcgatag aggcttcgac
     9421 aaaggcggtc agctgacctt ttgccaatgc gccgactttg gtggcgacga cttcgccgtt
     9481 tttgaacacc atcagggtcg gaatgccgcg cacgccgaaa cgggacgggg tggcttcgtt
     9541 gtcgtcgatg ttgattttga ccactttcag acggccttca aattcggcgg caatgtcgtc
     9601 caaaatcggg gcaatcattt tgcaggggcc gcaccacgga gcccaaaagt ccagcaggac
     9661 ggggatatct gcatttaaaa cgtctttttc aaaagcggca tcgctggtgt gtacaatcaa
     9721 ttcgctgctc attatttttc cttttcggtt gggattggca cgatagcgtg caggataggg
     9781 cttgggcata aaaatttcaa gtgggcgggc gggataatag tttttctgta ccgccccaat
     9841 cgcctttgca aattctaaaa aacggttcgg acagtatttt atttctgaaa attactttta
     9901 ttatcaaaat gtaaggaata cgtttgaggc tgtacagaag cgcgcgggcg gtgtatgctc
     9961 cgtttaaata gattcttaaa gtatgaatgt tcaaaaccaa acccgaaacc ctataaataa
    10021 aaggaaccct gccatgactg atttttccgt ttgggaaact gctccctttg gtgcaaccgt
    10081 cgatcatatc ctgcaacgct accacaatgt acaccgtgca caatttgaag aattggtgcc
    10141 gctggcgcaa aaagtggccc aagttcatgc cgataccttt ccggcggaaa ttgccgagct
    10201 gcttgcctat atgcagaacg aactgctgat gcatatgatg aaagaggaaa ggatgctgtt
    10261 tccgatgatt aatcagggtg tcggtcgggg tgcggcgatg cccatcagcg tgatgatgca
    10321 cgaacatgaa gaacacgacc gcgccatcgc aaggctcaaa gagctgaccg ataatttcca
    10381 agcgcccgaa ggagcctgcg gaagctggac gcggctttac gctttggcca aagaaatggt
    10441 ggaggatttg aacgaccaca ttcatttgga aaacgatatt ttgtttgccc gcgtactcga
    10501 ttcttaaggg ataggagccg gatatgtcaa tgccgtctga aacacggttt tccgcttcag
    10561 acggcatttc cacgtttcag cccaacaatt cgggcggaat gcggcacacg ggaatcgggt
    10621 tgattttgtg ctgcatcgcg cggtgcagcc gcgtatagat ttccagaact tcgcgctgcc
    10681 gcccttcaaa atcttcgggt ttgcgcgtgc cgtacacgcc cattgcccac tccagttcgg
    10741 gatagcttgc gcccatctgt tcttcgtcgg tgcgttccgt atcccacagg ccgtcggtcg
    10801 gcggggcttt ttgaatcgcc tcgtccacgc ccaatgcttc ggcaagccgg taaacctggg
    10861 ttttggtcag gtcggcaatc gggctgatgt ccacgccgcc gtcgccgtat ttggtaaaaa
    10921 atcctacacc gaaatcttca attttgttac ctgtccccgt aaccagtagt ccgtgtatct
    10981 gcccgtagta gtacagggtc agcatacgca ggcggcttct ggcgttggcg agggaaagcg
    11041 gctgattgtc aaatgccgtc tgatgaacgc cgacggtttg ttcaaaggtc tggaaggtgt
    11101 cggtcagatc gaccgtttgt gcgcttacat tggcatattg ccgttgcaga ttgcggatgt
    11161 gcagccttgc ccgctcaagc tggtcggggt gttggcgtat cggcatatcc agaagcagcg
    11221 tggtgcggcc ggtgcgggcg gcgagtgcgg agacgacggc ggaatcgatg ccgccggaaa
    11281 cgcccacgac aaaccctttt gcatttgccc gggcggcgta ttcgtccaac catcggacaa
    11341 tatgtgtgat aactgcctgc gtatccattt ttcctcctgt cgaaaatatc cgcatcatag
    11401 cttgtcggcg gtgtaatttg aatagattga gggcgggttg atgaatattt ttcccattcc
    11461 ccgcttaaaa aagtcatacg ggaaaaggcg ggcggcaaga tggcgcgtgt tataatattg
    11521 ttgacaattt aggcggacta ataccgcctt aggcttcaaa acatattcat attttttatt
    11581 atttgattga ttctatgtct gatttttttc attctgacgt gttgtccgtt tccgagttga
    11641 acgcatttgc caaaagtctt ttggaaaacc accttgccgg cctgtggatt gcgggcgaag
    11701 tgtccaacct gacccgtgcc gccagcggac attattattt ctcgctcaaa gacagccgcg
    11761 cgcaggtgcg ttgcgcgatg ttcaaaggtg cggcggcgcg tttggcgaag cctttgaaag
    11821 aaggcgacca tatcgaagta tcaggaaaaa tcagtattta cgaggcgcgg ggcgaatttc
    11881 aaattaccgt gaatgaggtg cggctcaaag gtttggggca gctttacgaa gcctacgagc
    11941 ggttgaaggc acagttgcag gcggaaggcg cgtttgcggc ggaacgcaaa aagccgttgc
    12001 ctacccgtcc gcaatgtatc ggcatcgtca ccagcctggc agcagcggca ttgcgcgatg
    12061 tcgttaccac cttgaaacgc cgcgcgcccg aaattcccgt tatcgtttat ccgacacctg
    12121 ttcaaggcgc aggcagcgag ttacaaattg cccaagcgat taaaaccgcc tcgcaacgcg
    12181 ccgaatgcga cgtgttgatt gtctgtcgcg gcggcggcag cattgaagac ttgtgggcgt
    12241 ttaacgaaga accggtcgtg cgcgcaatcg aagcctgcac ggttcccgtc gtcagtggcg
    12301 tgggacacga aaccgatttt acgcttgccg attttgtcgc cgacgtgcgc gcgcccacgc
    12361 cgaccggtgc ggcggagctg gtcagcccca accgccaaga atcgctacat cgtctcgctc
    12421 aggcgcaagg ccgtctgaaa accgttttgg aacaacgcta tttcgatgcc agccaaaaac
    12481 tcgactggct tgcgcggcaa atccgtcatc cgcgccaaaa acttgatgag cagcgcgctt
    12541 caatcggcaa gctggcgcaa acgctgtctt actcgatgaa gcaaaacctc cgtgcccaca
    12601 ccgcccgttt tgaacgccaa acccaagccc tgcaacatta ccgtcctgat gtttccgttt
    12661 gcaaaaacaa catcgtccgc cttcagacgg cattgcccgc cgccttcagc caactgctta
    12721 cccaccgccg ccaaagcctg accgcccaag ccgcattgct cgaagccgtt tcgccgcagc
    12781 acattctgga gcgcggcttt tccgtcgtca aaaacacacg cggacaagtc atccgcaacg
    12841 ccgatgtttt gaagcagggg cagaagctgc acattacctt cgccgacggc gaaaccgacg
    12901 tgcgcgtaag caaagaacaa gggcagcagg atttgttcga ttgcatttaa acccaatcgg
    12961 aaaaaccgct ccccctccgc cgtcattccc gcaaaagcgg gagtccagtc ccttgacctt
    13021 aagataatct ttaattcgtc attcctgaaa aagcgggaat ccggaagcga aaagtggtgg
    13081 gaatgtatta ga
//
LOCUS       DAXIHS010000057        13024 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXIHS010000057 DAXIHS010000000
VERSION     DAXIHS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.057"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(110..538)
                     /locus_tag="ACFOF2_001426"
     CDS             complement(110..538)
                     /locus_tag="ACFOF2_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC7107724.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="ACFOF2_001427"
     CDS             complement(603..2351)
                     /locus_tag="ACFOF2_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC7107725.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFOF2_001428"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFOF2_001428"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC7107726.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFOF2_001429"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFOF2_001429"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC7107727.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="ACFOF2_001430"
     CDS             4754..4924
                     /locus_tag="ACFOF2_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC7107728.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFOF2_001431"
                     /pseudo
     CDS             4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFOF2_001431"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5935..6279
                     /locus_tag="ACFOF2_001432"
                     /pseudo
     CDS             5935..6279
                     /locus_tag="ACFOF2_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6481..7890
                     /locus_tag="ACFOF2_001433"
     CDS             6481..7890
                     /locus_tag="ACFOF2_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC7107729.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="ACFOF2_001434"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="ACFOF2_001434"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC7107730.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEYCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFESQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKKEEAGEA"
     gene            complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="ACFOF2_001435"
     CDS             complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="ACFOF2_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC7107731.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
BASE COUNT         3218 a         3178 c         3475 g         3153 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacacgat ggatgaaatc
     6901 cacagcgata tgatgctcgg tgcggcaggc gattatcttt tggaaacggc agcatcgctg
     6961 acgattatca tgataatcag cggcttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg
     7081 tttggaactt gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg
     7141 ggtatttggg gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt
     7201 gtcgaaccga accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg
     7501 gtacatatcg accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg
     7621 agcgtgttgg cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgaccgcac tgcttgccat tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctgggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctc gtgtaccgtc ctcagtggat agacgcttgg cagcagggca
     8461 ggcggctcgg tttgggcgac gacgaaatct ggcagtccaa actgtggcgt tacctcgacg
     8521 atggcaggca gagcgcgccg caccgtgtcg cgttgtggga aaagctgttg gaatctttaa
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcaccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaat attgcgatgt gtttgtgttc gcactcaatc
     8701 cgagcgggat gtattggggc aatgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcggg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc aacacgggcg acggctcgat acgcatcgtc tccgcacaca
     9001 gccccttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa catcgaatcc tatacgcctt
     9121 ttatcgaagc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cagacgtgaa aatcagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgatatg gttgccgatt
     9361 tgaacgttca ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaacccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccctg ttcggacgtt
     9541 ttgccgccct cctcgaaacc ctgtcggata ttgtacggat atggcggcag cccgcaacga
     9601 tcggcgaatg ggtggcgcgt tgccgggatt tgcttgaaac attgttccaa gctgaagccg
     9661 atgaccaaaa gtcagtccaa aaccttgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccat atccgacgtt
     9781 tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgcgcccg ccgcgatgac gaccgctacc tcttcctcga agccctcatc agcgcgcgcg
    10021 aaatcctcta cctgtcctac atcgggcgcg acatccgcaa agacgaagag cttgctccgt
    10081 cttcgctgtt gggcgaactc atcgacacag ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaactggata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc actgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgaccaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gtttctggcg caaccccgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagtc gcaacacgcc gatcaaatcg ccgaaaccta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag cagaacttcc aaactgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag ccccaaactg ccaccgcttt catatgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaagacctc gcttgccgct gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc tattacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc actcgaacaa cccctgtttg aaaacctcgc ccgcctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaaag gaagaggccg gagaagcctg acgggataga aaggcaaatg
    11221 ccgtctgaaa gcgtttaagc atttcagacg gcattttgtt tatcgtgttt aaggtgcggg
    11281 atagaatgtg ggcgcaccgg gaccgacggg caggcccaaa acaaataccc aaatgcagaa
    11341 taaggcaatc catgcaatta agaagaaagc ggaatacggc aacatcatag aaatcagcgt
    11401 acccacgccc gcatcttttt tgtatttgat caccgtcgcc ataatcagcc cgaaataact
    11461 catcatcggc gtaataatat tggtaacgga atcaccgatg cggtaagcgg cttgaatgac
    11521 ttcgggcgcg tagccggcca acatcagcat agggacgaaa atcggcgcag ttaccgccca
    11581 ttgcgcggag gcggagccta tcatcagatt gataaaagca caaattaaaa taaaaccgat
    11641 aaacaacacg ctgccgccca agccgacttc ttttaagaac gtcgcccctt taacggcaat
    11701 atattgccca atattcgtcc aattaaaaaa tgcgacaaac tgtgcggcaa aaaagatgat
    11761 gaccaaataa agccccagag tactcatcga ttcggccatc gcattaacga cttcctgttc
    11821 gccgcgcaaa cttcgggtta cccggccata aacaatgccc ggcagcgcaa acaacaagaa
    11881 aataaaaaca acaatcgatt ttaaaaacgg cgaaccggaa accaatcctg tttcaggatg
    11941 acgcaaaata ccgtcggcag ggacgatgct ccaagccaat agggcggata aggcaacaaa
    12001 caccacgcca gcccaaatta atcctttata ttccaaaggc gtgatttcat tggaatgccg
    12061 aatgtctttt tcttcttgtg acaaatctga ttgataaggg cccaattgcg gttcgacgat
    12121 tttttcagta acaaaataac caatcaaagc aatcacaaac gtactggcta ccataaaaaa
    12181 ccagttggct tcagggccta cgacgtagtc gggatggatg atttgcgccg cctgttgggt
    12241 gatgcctgcc aagagcggat cgattgtgcc taagaacaga ttggccgaat aaccgcccga
    12301 aacgccggcg aaagccgcag ccagaccggc aagcggatgg cggccgaggg aatgaaagat
    12361 gatggcggac aaagggatta ggacgacata gcccaattca gaagcggtat tagataaaat
    12421 ccctgtaaaa acaaccataa aagtagtgag tttgcgtgga gattttgtga gcaataagcg
    12481 cattaatgcg gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa
    12541 caccgttccc aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat
    12601 caaaccgtca gcatcgagca ggctgacaac gtgaatcaaa ccgtcatcgg cacgtccttt
    12661 cgcaccaaca gggcgcggat cggggacgga tagtccgaaa tacgcaccgg cggcagaggc
    12721 aatcagcaat aacacaatga aaataataaa aagcgtaacc gggtgcggca acatattgcc
    12781 cagccattcg actgtgcgta aaaatcgtcc gtcccgttgc gtatcggttt gactcatctg
    12841 cttctcctta aaaaaaatat attgagtgca ttaaattttt gaatgtaaca tgaagtgttt
    12901 tgtaagcaaa ctaaaataat cgtatatgta tataaccgta atagattaaa acagaaaatg
    12961 cccacatcgt tattttcata aaggcggtaa tccgaaaact tgacgttaaa gccttatcag
    13021 gaat
//
LOCUS       DAXIHS010000058        12864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXIHS010000058 DAXIHS010000000
VERSION     DAXIHS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.058"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(231..386)
                     /gene="rpmG"
                     /locus_tag="ACFOF2_001436"
     CDS             complement(231..386)
                     /gene="rpmG"
                     /locus_tag="ACFOF2_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC7107732.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(418..651)
                     /gene="rpmB"
                     /locus_tag="ACFOF2_001437"
     CDS             complement(418..651)
                     /gene="rpmB"
                     /locus_tag="ACFOF2_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC7107733.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(901..2427)
                     /gene="farB"
                     /locus_tag="ACFOF2_001438"
     CDS             complement(901..2427)
                     /gene="farB"
                     /locus_tag="ACFOF2_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC7107734.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2451..3623)
                     /gene="farA"
                     /locus_tag="ACFOF2_001439"
     CDS             complement(2451..3623)
                     /gene="farA"
                     /locus_tag="ACFOF2_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC7107735.1"
                     /translation="MATHADETTIQNRQEANSAKVKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(3667..4026)
                     /locus_tag="ACFOF2_001440"
     CDS             complement(3667..4026)
                     /locus_tag="ACFOF2_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC7107736.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      4241..4285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            4289..4762
                     /gene="queF"
                     /locus_tag="ACFOF2_001441"
     CDS             4289..4762
                     /gene="queF"
                     /locus_tag="ACFOF2_001441"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC7107737.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            4839..5525
                     /locus_tag="ACFOF2_001442"
     CDS             4839..5525
                     /locus_tag="ACFOF2_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC7107738.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(6464..7756)
                     /locus_tag="ACFOF2_001443"
     CDS             complement(6464..7756)
                     /locus_tag="ACFOF2_001443"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC7107739.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            7833..8801
                     /locus_tag="ACFOF2_001444"
     CDS             7833..8801
                     /locus_tag="ACFOF2_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107740.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(9002..10468)
                     /locus_tag="ACFOF2_001445"
     CDS             complement(9002..10468)
                     /locus_tag="ACFOF2_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC7107741.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKVITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(10710..12836)
                     /locus_tag="ACFOF2_001446"
                     /pseudo
     CDS             complement(10710..12836)
                     /locus_tag="ACFOF2_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2955 a         3703 c         3123 g         3083 t
ORIGIN      
        1 gtattattat agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacaa
       61 atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc tctatccttg
      121 gcagacggtt tgcagcagat tcgcatacgg cagatgtttc aagcggaaaa gaaacacgat
      181 aaaaataaaa aagcctccga acagtcggag gctttgcttt caaactgaaa ttatttcagt
      241 ttggtttctt tgtacactac gtgtttgcgg gcaaccggat cgaatttttt aatttccagt
      301 ttgccgggca tagtgcgttt atttttggta gtggtgtaga agtgaccagt acctgcactg
      361 gattccagtt tgattttatc gcgcattgca gtaatcctta attaaatagt gtttaaatta
      421 agcttcgccg cgagcacgca aatcagccaa tacgacatca atgcctactt tgtcgatggt
      481 acgcagtgca gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt ctacccaaaa
      541 acgacgtgat tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg cgtgcgatac
      601 gttgttgccg gacatcgggc gtttgccggt cactttgcaa actcgtgcca taatcagtct
      661 ccaaacttct aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt agttcaagtg
      721 ttttaaagaa aaacggctgt tttgcgcgga taatccttcc gattgtcgca tctgtggaac
      781 gtttgcaggt gcgaaacaga tggggcgcga ttatagcgga tttcgggaac tgctgcattc
      841 aaaatgccgt ccgaacatat ttccagtatt ttcagacggc atttcaagtt ttcaaatccc
      901 tcaatgtcca ccgccgccgc cgttgtggaa cggcggtttt gccagccata tgacaggaat
      961 catgatgatg aacagcaggc tgcctgccat aaagatttca ttcgatccga tgatgaagcc
     1021 ctgctgggta atggtattgt tgatgatacc tagggtttga atgtcggaaa tgccttgttg
     1081 ggacaaatgc gccgccgttt cgtgcaatgt tgcggaatag ggcgtgatgt gttcggcaaa
     1141 gcgtgtgtgg tgcaacgctt cgcgccgttc ccacagggta ctgacgaatg atacgccgac
     1201 accgcccatc agcacgcgca agaaattcga caggctgccg gcggcggcaa tctgtccgcc
     1261 cttcatatgc gacagtgtga tggtggtcag cggcaggaag aacatggcaa cgcctgcccc
     1321 ctgccaaaac tgcggccaga tgacgttgcc gatatccatg tcggcataaa aatcggtgcg
     1381 ccaatagaaa gtgaaggcaa aagtcaggaa gctggcggtt acgagcaggc gcatatcgat
     1441 tttattgccg aacctgccga taatggggga caggaaaact gggaggatgc cgacgggcgc
     1501 ggcggcaagc cctgcccacg tggaggtata gcccaggttg gtctgcaaca ctaacggcag
     1561 cagggtcagc gtccccatat acaccataaa acccaatgac gtggcaatga cgccgacggt
     1621 aaaattccga tctttaaaca gcgataaatc gacaatcgga tatttttctc ccaattccca
     1681 aacaataaaa tacgacaagc acaccaatgc ggttacgccc aaggtaatga tttctcccga
     1741 agcgaaccag tcgagttcct tacccctgtc cagcatcatc tgtaacgcgc cgataccgac
     1801 taccatcaat gtaagcccga catagtcggt cggcatttta acggtttccg tttcccgata
     1861 tttcaaatgt ttccatgtaa tccatgccga tatgataccg atagggatat taatgaagaa
     1921 aatccaaccc caatgccagt ttccggaaat ccaaccgccg agtatcggcc cgagaacagg
     1981 ggcgacaacg acggtcattg cccacaacgc cagtgccagc gtccgttttg cgggcggata
     2041 ggatgccatt aacaggcttt gcgacaaggg aatcagcggc ccggcgataa agccctgcaa
     2101 gatgcggaaa ataaccagcg actgaaggtt gggcgcgatg ccgcacagcc acgatgtgat
     2161 gacgaaaccg acagcggcgg cggtaaacaa tttgacctcg ccgatacgtt ttgccaaaaa
     2221 gcccgttaac ggcacggaaa cggcgtttgc cacagcaaaa gaagtaatga cccacgtccc
     2281 ctgagtggtt gccgcaccga ggttgccggc gataacggga acggcgacat tggcgatagt
     2341 cgtatctaaa acttccataa atacggcaag ccccaaagac agcgtaatcc atgccaatgc
     2401 cgcaccctta agcggtggat aatccattgt gtcctccgtt tcagacggca tcagggcgcg
     2461 gattgcccga ggatttcatc gaccgtccga tcgacttccg accagtcggt actttccatt
     2521 tccggcaatg ccgcacccgg cgtttttgaa acaggcgcgc ctgcggcgga agtatccact
     2581 ttaaccgtca tcgacaaacc gatacgcaac ggatgcctgt ccacatcttc gcggttcagc
     2641 acgatacgga cggggacgcg ctgcaccact ttaatccagt tgcccgttgc gttttgcgcc
     2701 ggaatcagcg aaaacgcgct gcccgtacct gccgaaaaac ctgccaccct gccgcgataa
     2761 acaatttgtt tgccgtacaa atcggacacc agctcggcag gctgtccgat tttcatatgc
     2821 cgcaactgcg tctctttaaa attggcatcc acccacacat ccgacagcgg caccaccgcc
     2881 atcagcggcg cgcctgccgc cacctgctgc ccgacctgca ccgaacgctt cgccacctga
     2941 ccgtccgccg gcgcgcggat ttgcgtccgc tgaaggttca accacgcatc tttcaacctg
     3001 ccgattgccg tctgaaccgc cggctgttcg cgcaaagaaa cctgaccgcc caaagccgcg
     3061 cgtgccgaag actcttccgc caaagccgcc ttaactgccg cctgcgcctg agacactgcc
     3121 gcacgggcgt gtgccagctc ttcggcggac accgcgcccg attccgccaa agcagaccgg
     3181 cggcgcaaat catcctgcgc gcgtgccaaa tccgcacggc gcaaggcaac ctgcgccccc
     3241 gcctgcgaag tggcggcatt ttgccggcgg ttttgccgca ccgcctgaac cagctcgttt
     3301 tttgcccgct cgtaagccag cacatcatta tcgtcgtcca ataccgccag cacgtcgcct
     3361 tttttcacgg catccgtatc gtcgtgcaaa accttccgta ccgtaccgcc cttttgcggc
     3421 gtaacctgaa ccacgcgtcc ggcaacataa gcgtcttccg tttcctcttc gtgctgccac
     3481 cataaaaaaa acgccgaccc ggcggctgcg gcggcaagcg cgaacagcag cgtcaatgcc
     3541 gtcaggcggc gtttacgttt gacttttgcg ctattggctt cttgtctgtt ttgaattgtc
     3601 gtttcatctg catgtgttgc cattattcca attccaaata cttctaatac tatgtttttg
     3661 atttaattag cactgataat cttcaaattc ccacgaaaaa aaacgaagta aataagtcaa
     3721 tgacttttcc caagtttctt ttgaacattc tttaagaatt ttctcaattt ccgatttaat
     3781 aacagaatga ttaaattcat tcataatcat catacccgct ccccatttaa ccctttgatt
     3841 ttggaaacaa ttatgcaaaa tccatttagg agagcatatg cgaacagaaa atatatctgc
     3901 agcatcacta tcatcagttc ctatgtctaa atcaattccc acacaaaaat tgtctttgat
     3961 ttcgggaacg aaatcttcaa aggcacaatc gtaaagattg atggctttca attctaggtt
     4021 aatcatttta tattcaaaat aaataaaacc aattaaagat tggttaggat tctataccct
     4081 ggcaataact caatcaagtt aatgatttag aatgaatatt caaataaact gtatcagccc
     4141 ggcctcacag aactatttat atccacacat caccgagtaa agcaattacc catactaacc
     4201 gaagaaaact acacaaatac cgccacttat attacaatcg ccgcccgtgg ttcgaaaacc
     4261 tcccacacta aaaaactaag gaaaccctat gtcccgcaat accgaagagc tgcaaggcat
     4321 ctcgcttttg ggtaatcaga aaacccgata tccgaccggc tacgcgcctg aaattctcga
     4381 agcattcgac aacaaacatc ccgacaacga ctatttcgtc aaattcgtct gcccagagtt
     4441 caccagcctc tgccccatga ccgggcagcc cgacttcgcc accatctaca tccgctacat
     4501 tccgcacatc aaaatggtgg aaagcaaatc cctgaaactc tacctcttca gcttccgcaa
     4561 ccacggcgat tttcatgaag actgcgtcaa catcatcatg aaagacctca ttgccctgat
     4621 ggatccgaaa tacatcgaag tattcggcga gttcacaccg cgcggcggca tcgccattca
     4681 tcctttcgcc aattacggca aagcaggcac cgagtttgaa gcattggcgc gtaaacgcct
     4741 gtttgagcac gacgcacaat aaaccatcct tcccatattt cagacggcat tccttcgata
     4801 cgatatgccg tctgaacgcc tttccgaaag gtttttttat gtacgcattt accgacgcac
     4861 agcaacagaa ggcactcttc tggctggtgc tttttcatat cctcatcatc gccgccagca
     4921 actatctggt gcagttcccc ttccaaattt tcggcatcca caccacttgg ggcgcatttt
     4981 cctttccctt catcttcctt gccaccgacc tgaccgtccg cattttcggt tcgcacttgg
     5041 cacggcggat tatcttttgg gtgatgttcc ccgccctttt gctttcctac atcttttccg
     5101 ttttgttcca caacggcagt tggacaggct tgggcgcgct gtccgaattc aacacctttg
     5161 tcggacgcat cgccttagcc agctttgccg cctatgcgct cggacaaatc cttgatattt
     5221 ttgtattcaa caaattacgc cgtctgaaag cgtggtggat tgccccaacc gcatcaaccg
     5281 tcatcggcaa cgccttggat acgttggtat ttttcgccgt tgccttttac gccagcagcg
     5341 atggatttat ggcggcaaac tggcagggca tcgccttggt cgattacctg ttcaaactca
     5401 ccgtctgcgg tctgtttttc ctgcccgcct acggcgtgat tctgaatctg ctgacgaaaa
     5461 aactgacgac cctgcaaacc aaacaggcac aagaccgccc cgcgccctcg ctgcaaaatc
     5521 cgtaaaacag ccctgccggg agtcgggacg gttgggtttt cttttatttg caaacactgt
     5581 cggaacggat aaatccggat tcccgcccac ttcgtcattc ccgcgaaagc gggaatccgg
     5641 tgcgttgggt ttcagtcatt tccgataaac tcctgccgcg ttgtatttct agattcccgc
     5701 tttcgcggga atgacggcgg atgggtttcc gtgccgtggt gtccggattc ccgcccgtac
     5761 gggaatgacg gcagtgcttc cttggcttaa ttttatttta atcgactgta tggtggatta
     5821 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
     5881 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
     5941 ctggtttaaa tttaatccac tatatttgca aacactgcca gaacggataa atccggattc
     6001 ccgcccactt cgtcattccc gacgcgcacg ccgctttccc gcccacttcg tcattcccgc
     6061 gaaagcggga atccggtgcg ttgggtttca gtcatttccg ataaactcct gccgcattgt
     6121 atttctagat tcccgctttc gcgggaatga cggcggatgg gtttccgtac cgtggtgtcc
     6181 ggattcccga cggtacggga atgacggcag tgcttcctta acttagtttt attttaatcg
     6241 actgtatggt ggattaacaa aaatcaggac aaagcgacga agccgcagac agtacaaata
     6301 gtacgaaacc gattcacttg gtgcttcagc atcttagaga atcgttctct ttgagctaag
     6361 gcgaggcaac gacgtactgg tttttgttaa tccactataa atgccgtctg aaaacctgtt
     6421 tgcaggtttt cagacggcat tgttttcgtc gccggttcaa acttcaatcc gattgcgaca
     6481 cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg
     6541 caaacgccgc cttgtccgcc tgcgtcaatt cgggtgtcgg caatgcgact gaaacaggat
     6601 taacgggctg cccgttgatg cgcgcctcgt aatgcaggtg cggcccggtc gaacgcccgg
     6661 tcgaaccgac aaaaccgatg acctcgccgc cgcgcacatt gccttgtgcc tgcgaaaacg
     6721 cgctcaagtg cgcgtacagc gtttccacgc cgttggcgtg gcgtatcatc accgcgttgc
     6781 cgtatccgcc cttccggcct ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg
     6841 ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg ccatgtgtgc aggatggggt
     6901 gcatacggta gccgaacggc gaagaaatgc gcgtatagac cagcggctcg atgttgaagc
     6961 cgcctttttc ctgcaacacc ctgccgtctt catcataata attgccgccc cctccgcctt
     7021 ccttgtccga acggtaatag aatgcctgat gccttgtgcc gcccttaacg acttcagccg
     7081 ccaaaatatc gcccgccgcc acctgctgcc cgtggaaata caggctgtcg tacatcaggc
     7141 gcacggcatc gccttccttc aaaccgtcaa ggctgaagcg gccggcgaaa atcccgctta
     7201 aggattcgcg gatttcgacg ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt
     7261 tgaccacgac cgaacgcagc gtcggcaaaa ccttcatatc cgcctcagaa gccgaccgcc
     7321 gccatatgcc gcctttcttt tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa
     7381 aaaactgtac ttcgcgcgcg ccgccgtcgc cgccgaccaa aacatgaacc gactggtcgg
     7441 cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc cgtgattcgg gcaatctcgt
     7501 cccgcgccat acccgaacgc gccagcacgt ccgccagcga gtcgcccggc tgcaccgcct
     7561 cctgcaccca atatgccgtc tgaacgccgc tgccgcccca agacagcggc ggcagatttt
     7621 gttccacgcg ctgcggtctg acgcgttccg tcctctctgt caaagcaatg tatgccgccg
     7681 acaccaaaat aatcgaaacg gcaagcgcgc gcagcgcgta tttccgatgt tttgccgaaa
     7741 gtgggaagac agccatattg ttcctttttg acggcgtttg cgccgaatcc tctataatat
     7801 ccgcccgatc cgcatttgtc agacatcaaa cgatgaaact caccctcgcc ctcccctcgc
     7861 tcaatttgga tgaagacgaa atccgaatac ctctatgcct gcccgccttc aacaaaatcc
     7921 tgcaatacgg ctcgctgcac cgccaaagct gcacggcttc ggcattttac gcgcgttatt
     7981 tgtggtgcgg cagactggcg gaacgcgcgg cgcaaagcct gaatatgccg tctgaaaccg
     8041 tcgcgctcgc cacgcccgtc tggcaaaaga tggggatgca tcaggcaaat gtgctgacgg
     8101 cggaatattt ggatgtcggt acggatgaag cggaacggct ctgccgcgat ctgtcggcgt
     8161 tttacggcga tattccgtgg cgtttcgttc ccgtcctgcc cgaattatgg ctggtttccc
     8221 ttccccgcgc gtaccgctgg ggcgcgaagc ccgttttgga cttgggcgga ttgttgggcg
     8281 cggacgatca gccggacgga gaagatgcgt tggaatggct gcgcgtccaa accgaaatcc
     8341 aaatgtggct ggcggcgcac cccgtcaacc ataaccgcaa gaaacgcggg ctgcccgagt
     8401 tgaacggctt atggctttgg ggcggcacaa acggcggcac gcaaggcggc acgcttttcg
     8461 ccgatacggt ttggagccgt ttccaccccg accgccgcgc attgcccgac aacttccgcg
     8521 cctatgaaga aacggcggca cacctgcccg acacgcacca tatcctgttt atggacgatt
     8581 tgcgcctgac cgccctgaca ggggacaggg agcggtatgc ggcaatattg cagcagtggg
     8641 aagaacgctg gttcgcaccg ctttacgaag ccgtccgcat cggcaaaatc aaacggctgg
     8701 acatcgccac ggacggacaa cacggcggta cgctgacctt caaacccgcc gacaggtgga
     8761 aattttggcg cggcgcgaaa acctttgacg gaatctggtg aacccgcccg ccgcaaccgc
     8821 gacaatgccg tctgaagccc gaatcgggtt tcagacggca ttttacctcg catcgccgtt
     8881 atgccggaaa aaccggaatg cgtttccgca cacgcggcgg cacgcaaagc accgcccgaa
     8941 agccgccgat gcccgccgtg ataccgccgc cggcagggtt tcctccgaaa aggaacagca
     9001 atcagaacgt tttattaaac tcgacaaacg cccgattttt ctcgtattcg ttgaacacat
     9061 cgttactcca cgtttcgcgg tgcgacaacg tcaggcgcgg cgtgatgact ttgaaatgca
     9121 atgcccggtg ccaaaggctc aaggatgtgt tcaattcttt atccctgcgc ctttcccctt
     9181 taaaaccgct gaaaaagccg ggtttttcat aatgccgttt cgccgcgccg aggcgcaaca
     9241 gcgaagacag gccgctgccg ccccattcct gcccccaggc aaagcgcagg ccgtaacggt
     9301 tgaaattgtc gccccggtcg gcggggttgc gctcgcggta aaaatccaaa ccgcccatcc
     9361 aatattggcg cgcattccgg taaaacacca gcgaattgga aatttgcaaa tgggtattgt
     9421 cggaacgcgc ccggcgcgta ttcttcaaac gcccccactc cgccgaagac aacgtttgcc
     9481 atttcggggt ttgccaacgg ttgaaataaa ggcgtgcgcc gttggtgtaa gaataagcgt
     9541 cgttgccgta ggtgcggcgt tcgtggaaca ctgccagccc ggcatctttg cgccggtcgg
     9601 caaaaccgat gccgccggaa acgcctgccg tcatatcgtt gaatttctta ttccccggat
     9661 aaaccctgcc ggacacgtcg ccgcccgccg tcgtgtacca gccgtttttc agcgaccatt
     9721 ttttctccgc gccgagccgg taattgaccg ccgtgccgtc cacctgtttc gggaaagtcc
     9781 atttgccgta ctgctgccgt ttcggggctt ggttgatatt gtgttcgcgg gtgacgctga
     9841 agccgccatt taccttccac gcatcgcgtt cgcgcaatgc cttgcggtac agctcgacct
     9901 gctccatcag ctgcggcggc aggttttccg ccttcaggcg gtcgaactgg tctgccgccg
     9961 cctcgttctg cctgttttca aacaatgccg ccgccaaacg catacggacg gcgggcgcgt
    10021 cgggttgggc ggcaatcaat tcccggtaat gggaaatcgc ctccttcacc ctaccgtctg
    10081 cctgcgccaa aatcccttgt gcataaagtg ccaacatctt atcctgctgc gcctgttgta
    10141 ggtaaatcgg caaaataacg cggataccgg caatattgtt tgagaccact gcggaataca
    10201 tcgcgcggga caacaattcg ggatttttca gcagggtttc tccgtcaatc tgcaaaactt
    10261 ttcctttttc ccgcacctgc cccggtacct tctccctgtc gattggtttg acctccgctt
    10321 catgaagcct gaactcggga cggcttctca aatccggttc gcgcggtgtt tcttcggcat
    10381 atgccgcgct tgccaataaa ggcagcagca gcatccatct gtttcgtgca ggcataaatg
    10441 tcttcccaca aaaataaaaa ataaccatta tcaatcggcg gattgtatca gttttttatg
    10501 ccgctgttcc aaaccctgcc gcccgatgcc gcgacaatgc cgtctgaagc cagaatcggg
    10561 tttcaaacgg cggcaaaaaa ccggcggttt tccgccggtt tcatcatgat gccccgccct
    10621 tcggggcggc agggttcgga ttggtttgcg gggcggcgcg cggggccgtg ccggtgtgcc
    10681 gcctgtgcgt caaaacattt cccgacgcat caaaacagcc atttgagcga caatgcggct
    10741 tctttgtcgc cgtccgtcct tttgccgtat ccgatgcgtg cggacatatg gcctttccaa
    10801 ccggcttcaa tgccgaaccg cccttcgagc gccgtcctgc ctgccagcgt ctgtttttcg
    10861 ccgtccattt ccacgccgaa agattttgac ctgtgcaaaa cattaaaagc ggcgaaaggc
    10921 tgaagattga caccgttacg caaagcaaaa cgggtttttg cccgaatacc ggcgcggctt
    10981 tgccactgac cgctgccgag cagtccgacc gccgtcccct cgctgtcggt aaagccgccg
    11041 tttacgccca agtaggtaaa ctgagcctgc ggttgcaggt aaaaccgcac attattgcct
    11101 tttccgacaa cgccttccgc cacaagcgcg ttgtagccgc cttcgacaga agccgtccaa
    11161 cctttggttt tgtagcgttc cacacggttt tcatcattga tgcggtgttt gaaacgttgg
    11221 tattgcaacc agccgtccaa atacgcaccc gtttgtttat cgcgcaactg atgccacgca
    11281 gcataaacac ccccgccata accatgcaaa taactgcctg ccgcaccgcc tttgccgttg
    11341 actgatgcgt gctggccggc cctgccgccc atcacgccga ttgccagccg gctgccttca
    11401 ttttgccgta caaacacctc gccgccgatt tgcacgcctt tgcgccgccc gtccgcagcc
    11461 gcgccgcccc gtatattttg atgcgaccgg ccgccgatga agcgcagcca caatttttga
    11521 cgggacggat cggcggcata cacgtcgtct cccctgtcgg cggcacgcag cccaaacaaa
    11581 gtatttgccg cataagcctg ttgggcatat aaaaccgctt ccgcctcggg gatagccaaa
    11641 cgctgcacgc ccaagcaaaa tccgccttcg cattgcttga gcaataattc gtacagtccc
    11701 tgccggtaat tttgtttaaa cgcaaacgaa ttagggtctg ccttttccgc cgcaatcagt
    11761 ttgcgcccgt cgagcgtttt cagattttcc ggccggcgtt ccaaaaccac gccggattgt
    11821 ccgctgactt tgtcttcaaa ggtcagggcg ggttcgtccg actcccgcac gtcgtagccg
    11881 aacaggaaat ccgcgccgcc ctgatacgct ttatggacgg tcagtttttc atctttcgtg
    11941 ttcaggcgga tccgggcatt atcggaagtt tccagcatat ggatgtcgga atggcggcgc
    12001 ggttcccaaa gggaatcttt cagcgtcatc ttggtcagtt ccaacacctg tccggcgacg
    12061 tgggatttgt tttcgatttt gacgtgtaat tttttattga agatgttgtt gagactagtc
    12121 tgtgatgtat tactatgagg agtgagtacg gaatcttcta ccaatttatt aattaaatcg
    12181 gggtttttct gtttgagtat actaaatttt ggtccaagct gttctatata cgcctcctca
    12241 gtccgttttt tattttcttc ccaagcttcg ggtcttgttt tgtataaatc ctgtaattgc
    12301 ttcttaaccg catcctttaa attatccccg taagtatctg taatatattt cttaacaacg
    12361 ggcatattat cgacggcgag tatgccgcta ttgtagtttt taccttggat attgatttcg
    12421 tcatacccat attcggatga attttgatcg gttttaccgc tggaaaaata atgggaagtg
    12481 ccgggtttcg gcttgtctga aaatgaaaaa tcggcatcgt cgccgatttt ggtagaacga
    12541 gtatttgaac caagctgagc tctgaacata ccgaagcaat caaaagatat gctgcacggg
    12601 ccgcgccaaa cgtgggaatt tctgtcttcc gccgcttcta aaataaattt gtttttaatc
    12661 tcggcttctt tgacgggatc gtacgcatcc gaacctcaaa catatggcgt gcaaaacagc
    12721 gataaaacag ataatgttaa tagattaacc cgaccgcttg cttgcttgct tgcttgcttg
    12781 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttt cagtgtcatg agaaattttc
    12841 ctttgtcaag tgttaaaatt ataa
//
LOCUS       DAXIHS010000059        12659 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXIHS010000059 DAXIHS010000000
VERSION     DAXIHS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.059"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(84..737)
                     /locus_tag="ACFOF2_001447"
     CDS             complement(84..737)
                     /locus_tag="ACFOF2_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC7107742.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(769..1281)
                     /gene="coaD"
                     /locus_tag="ACFOF2_001448"
     CDS             complement(769..1281)
                     /gene="coaD"
                     /locus_tag="ACFOF2_001448"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC7107743.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1405..2088)
                     /locus_tag="ACFOF2_001449"
     CDS             complement(1405..2088)
                     /locus_tag="ACFOF2_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC7107744.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2245..2511)
                     /locus_tag="ACFOF2_001450"
     CDS             complement(2245..2511)
                     /locus_tag="ACFOF2_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC7107745.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="ACFOF2_001451"
     CDS             complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="ACFOF2_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC7107746.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="ACFOF2_001452"
     CDS             complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="ACFOF2_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC7107747.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3553..4146)
                     /gene="nadD"
                     /locus_tag="ACFOF2_001453"
     CDS             complement(3553..4146)
                     /gene="nadD"
                     /locus_tag="ACFOF2_001453"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC7107748.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4143..4742)
                     /locus_tag="ACFOF2_001454"
     CDS             complement(4143..4742)
                     /locus_tag="ACFOF2_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC7107749.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(4771..6312)
                     /locus_tag="ACFOF2_001455"
     CDS             complement(4771..6312)
                     /locus_tag="ACFOF2_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC7107750.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            6705..8090
                     /locus_tag="ACFOF2_001456"
     CDS             6705..8090
                     /locus_tag="ACFOF2_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC7107751.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8110..8628
                     /locus_tag="ACFOF2_001457"
     CDS             8110..8628
                     /locus_tag="ACFOF2_001457"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC7107752.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8967..9884)
                     /gene="thrB"
                     /locus_tag="ACFOF2_001458"
     CDS             complement(8967..9884)
                     /gene="thrB"
                     /locus_tag="ACFOF2_001458"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC7107753.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(9921..10649)
                     /gene="ubiG"
                     /locus_tag="ACFOF2_001459"
     CDS             complement(9921..10649)
                     /gene="ubiG"
                     /locus_tag="ACFOF2_001459"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC7107754.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10801..12042
                     /locus_tag="ACFOF2_001460"
     CDS             10801..12042
                     /locus_tag="ACFOF2_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC7107755.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            12192..>12659
                     /locus_tag="ACFOF2_001461"
     CDS             12192..>12659
                     /locus_tag="ACFOF2_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC7107756.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMQRELVGLYGVPP"
BASE COUNT         2921 a         3787 c         3206 g         2745 t
ORIGIN      
        1 tgaagagatt tgcagtcaac cgttttcttg aaaaattttt ataaaaatat ttatcatatt
       61 gtttataaat taatttatat ttattaacct gcttttgcca tataaaattc ttccagcgca
      121 tgagagatat tttcagtctg ccattcgttt tttaagggtg caacaatttc gatttgtcgg
      181 ttttggtagg caaattgtat tttccatgca tacagaaaca tggtttcgga ttctgttccg
      241 ccgtataagc tgtcgcccag aatcggactg cccaaacttt tcatcgccac tctcaattgg
      301 tgcgttttgc ccgtatgcgg ttctaggatg aacagccgca gtttttcggc gatactgatg
      361 ctgtggaatc gggtaacggc gatattttct gtattgcgcg tcaacttcca cattccacat
      421 ctggattttt ccattccgcc tttaatccaa ccctgctttt tggacggctt gcggtcggac
      481 agtgccaaat aggttttttt gatgcttttg ccggcaaact gtccgctaag ggcggacgcg
      541 ctttctttgt tgagggcaaa cagtaaaatg ccgctggtct gtttgtccaa tcggtgcagc
      601 agccacacac gctctacgcc caactgtatg gcgagtgttc gggccagtcc ggtctcgccg
      661 ctgtcttggt ggacggatat gccgcccggt ttgttgatgg cgacgaagtc ttgatggcgg
      721 aacaaaattt ccaacatatc catatatgcc ttgcaaaaat agaagggttc aattttcgtg
      781 ttgatgttcg gcaaggattt tttcgtacac agcttgcggc acgtagcggt ggatcgtttc
      841 cgtccagcct tccggcccga ccagtccttt gaccatagtg gacgacactt cggcgatttc
      901 gcgcggcggc atgaggaata cggtggatat ttcgggggcg aggtcgctgt tgatatggcg
      961 catggaacgt tcgtattcgt aatccgaagc agaacggatg ccgcgcacga tgaatcctgc
     1021 atctacctca cgggcgtaat gcaccaaaaa tcggttttca aatacatcgg ttctgacgtt
     1081 gggaaacatt ttagtaatat cgcacaacat atcctgcctt tcagcgacgg tataggtgct
     1141 gcgtttgtcg gggttaatgc cgatggcgac gatgagttcg tcaaacatag attgcgcctg
     1201 ccgtatcatc cacagatgcc ccaatgtggg cggatcgaaa ctgccggcat aaacggcgcg
     1261 gcgcggtgta ttcggtaaca tttgattcct cccggcttca tagtcggctg tgtgttggtg
     1321 cgtgtgcatc cgtattgtat gcccaaagta aaatgccgtc tgaagcagtt tcagacggca
     1381 tagtcggacg gcgttttacc ggcatcaatc ctcgccgttt aaagacaaca ggatgttcag
     1441 caggctgctg aagatgttgt aaagcgagat aaacagtgtc agtgccgcgc tgatgtggct
     1501 gtcttcgccg ccgtcgatga cggtgcgtac ctgccacata atcattaagg aactgaacaa
     1561 gacaaaaccg gcggaaatgg tcagggcgag tgcgggaata cccaaaaaca gattggcaac
     1621 cacggcgacc atcagaatga ccgcacctac ggtcaggaag cgtccgagcg cgttcatatc
     1681 gagccgggtt cggcgcgcca aggcggacat cgttaaaaag acggcggcgg tcatcgcggc
     1741 ggcaatgccg acgattttcg caccgtcggc aatatggagc gcgtattgca gcacggggcc
     1801 gatcaatacg cccataccga atgtgaatac catcagcagg gtaacgccgg tattgctgta
     1861 acggtttttc tcgatgaagt ggatcatacc gtagaaaaac gccaacacga cggcaaaccc
     1921 tatccagcgc gaaccgaagg cggcgtaaaa attgaaaccg gcattggcgg caagtgccgc
     1981 gcctgcggaa gccggaataa atgaaaatcc gagcaggcgg taggttttct gcaggacggt
     2041 gtttttagaa accgtatgcg cggtgtagtc gtaaacgtcg tgttgcatat catctgctcc
     2101 tgaaagcgcg gttgggaata atgggggatt ttaacattgc ccaatgtcaa aatttgtccg
     2161 gttgcgtgaa gataaagttg tccggcgtat tttaaaggcc gtctgaagca gtttcggaca
     2221 gcctgtgttc aaaacggaaa accgttattg cggaacgtat ccctgaacgg catccgcgcc
     2281 gtcgccgaag aaatactgct ccatctgctg agccaggtat tcgcgcgcgc gcggatcggc
     2341 gaggcttaaa cggttttcgt taatcagcat cgtttggtgg cgcgtccacg ccgcccacgc
     2401 ttcttgcgat acgttttcaa aaatgcgctt gcccaattcg ttgggaagcg gcggaaattt
     2461 catgccttcg gcttctttgt tgagcttgac gcagaatacc atgcgtgcca tagcggattc
     2521 ctttgctgtg ttcagaaata acggggtgat tttaaccgat tagggatacg gacaaaagcc
     2581 ttcttattcc cgatgatagg gatggttgtg caggatggaa acggcgcggt agagctgctc
     2641 ggtcagaaag acgcgcacca tgccgtgcgg cagggtgagg ctggacaggc gcatcatcat
     2701 gcgggcttgt tgtttgaggc ggtcggtcat gccgtccgcg ccgccgatga cgaagcagac
     2761 gtgttcgccg ttttgccgcc agcttttgag gtgttccgcc agctcgacgg aggtcggcgc
     2821 tttgccgcgt tcgtcaagaa cgacgaggaa cgcgccttgc ggaacggctt cgaggatgcg
     2881 tttttcttcc gccgccatac cttgggcggc attcacgccc gcgccgcgtt tttcgggttt
     2941 gatttctttg agtgcgtagg cgacgtcgcg tccgaagcgt ttggcgtatt cggcgacggc
     3001 ctcatcaacc cagcgcggca ttttggtgcc gactgccaaa acggtaatgt tcaatgcttt
     3061 ctcccttaca ggaaaatgcc gtctgaacat tcagacggca tcgggaatca gtctgccgcg
     3121 tgccacggct tctgcattcc ggcgtgaaaa ctcggtttct cgccgcccca aagggtgtcg
     3181 atatcataga agtcgcgcac ggcggggagc ataacgtgga cgacgaggtc tcctgcatca
     3241 acgagcgtcc attcgccgct gtctccttcg gtactgagga tttcaaaacc ggcttctttc
     3301 aaatcgacgg caacgttgtt ggccagtgct ttgacttggc gcgtactgtc gccgctggcg
     3361 ataatcattc tggcaaacag cgaagttttg tcttgcgttt cgagaacgga aatgtctttg
     3421 gctttgatgt cttcgagggc gttgacggcg accccgacca ttttttgcag gtcttgcagt
     3481 tcttgttcgt tcattatttt cctaacggga tgttttcaga cggcattata gccgtttctt
     3541 actgatttga ctttattttt catacaaacc gtgttcgcgg atgtagcgtg cggcggcagg
     3601 tgggatgccg tctgaaacac cggcgcggcg gatttccgtt gacgacacat tatgcatcgg
     3661 ggcggacaag atgcggacgc tgccgtcctg aagggacttg cccagccacg cgtgcagttc
     3721 gcgcggggtt tggtgcaggc tgtcgccctg ccgcatggcg acggcgatat tggtttcgcg
     3781 cacgagcatc tgccattttt tccatgtgtg cagcttcatc aggctgtcgc tgcccatcag
     3841 ccaccagagt tgcgcggatg ggaactgctg gcggaagatt tggacggtat caaaagtata
     3901 ggttgcacct tctcggacga tgtcgcaatc gctgacggca aaacgcgcgt cttctgccgt
     3961 cgccagttcg accatggcaa ggcggtcggc ggcggaagcg gaggctgcgt ctttgtgata
     4021 cgggccgcct gccggcagga aaacaaccgc gtccaagccg atttcgtcgg caaaggcgcg
     4081 ggcgatgtgg agatgcccgt tgtgtatcgg gtcgaaagtg ccgccgaaca gtccgatttt
     4141 cttcatgatg tttccattcc ttccgataag tccatgccgt ctgaaacact gccgtccaca
     4201 accagcgtca gtttgccggt aacgggatgg atgaccagtc cgtgaacggc gatatggcgc
     4261 ggcatcagcg gatggttacg gataaggtcc accgtgtggc gcacgctgtc ttcgacgttg
     4321 tcgaaaccgg tcagccagcc gtcgaggtcg ataccggcat aacgcagggt ttcgatacgg
     4381 tcttcgggaa tctggctttc ccggacgcgc ccgaggaatt cttcggcatt cagcccctgc
     4441 ataccgcaat cgtgatgggc gatgaccata atctctctga ccttcaattc aaacacggca
     4501 accaaaagac tccgcatcac cgaaccccac gggtgcgtaa ccagcgcgcc ggcattttta
     4561 atcagcttgg catcgccgtt tttcaaaccc aacgcgtcgg gcagcagccc gataatccgc
     4621 gcatccatac aggacaaaac tgccagcccg cgttcggggt atttgtcggt aaagtatttt
     4681 tcatattcgc ccgactcgac aaactgccgg ttatgggcaa ggatgttatc caactcgctc
     4741 attttgccgt cctctgaaaa agggttcaca ttataacgtt tccgtctgtt ttccgccttc
     4801 gccgttgtcc aacagcagga aaatgcccag cgcgaacgcc gacaacagca atgtcaacac
     4861 cattgcccgc gcataattat cctcacccgc acgccccaaa taggcataaa tcaaagtcgt
     4921 cagcgtctgc cactcgggac gcgacaggaa caaggttgcc gcaaattcgc ccacgcaggt
     4981 tgccgccgcc aaagtcagac cgcgccgcaa cgccggtttc aagaggggga acgtgatgcg
     5041 gcatgccgtc tgaaagccgt ttgcacccaa acccgccgcc gccctgccgt aatccggcgg
     5101 cagtgcatcc caggctgata aaacatcttt tgccacaaac ggatacgcca gcagcgcata
     5161 catcgccagc agcagcggca acgaagccgt ccactgcgga taaagcagca gcacgcccgc
     5221 cgaaacacaa accggcgaca ccataaacgg caaaaacatc agcccgcgca tccacgccga
     5281 ccgccgcgcc gccgccgcat acaccacacc caaaaccgcc gccgcataca ccgccgccgc
     5341 cgagaagcgc aaagtattcc acaccgcctg ccacgtttca ctttccatta acacacgcca
     5401 cgattcgccg gccgaccacg ctttcacaac aattgccaac aaaggaaaca ggcagcacac
     5461 agacaacacc gccgccgcaa acgccagcag cacatattcc ccgaccgact gcggcggcga
     5521 cggcatcaca ggggaaaccg ccttatccga aaccgcgcgc ctgccgaacc acgcatacag
     5581 caaccctgcc gccgccgtta cccccaacac cagccacacc agcaccgaag caaccgccat
     5641 atcgagttcg aacatgacca actggtaaat ttccacttcg accgtggcat aacggctgcc
     5701 gcccagcagc aatgccagcc cgaaccccga aaaacaatac aggaagacaa ggcacacgcc
     5761 gccggcaagc cacgggcgca aaacgggcat ttcaatgtcc aaaaaccgcc gccacgcccc
     5821 cgcgcccaac gtccgtgccg tctgaagccg tgccgcaggc acttgcacaa acccctgata
     5881 cgccgccctg accaacacag gaaggttgaa aaacacattg ccgtacaaca acagatacgg
     5941 cgtatcctgc ctgccgcgcc acaacagccc gtccgccccg aacagggcca gcacgcccac
     6001 gcccgccacc aacgtgggca tcacaaaagg cagcatcagc aggcgcagca ccaaagcccg
     6061 ccccggaaac gccagccgcg ccagcaccca cgcgacaggc acgcccaaag gcagcaccag
     6121 cacacaggtt gccgctgcct gaaataccgt ccacgccaaa cgtttgagca tataggcatc
     6181 cgacagcacc gcgcgccacg ccaaaccgtc atacgccgcc accgcccaca aaggcgcaac
     6241 gaccattacc gccaaaaaag ccgaaggcag cagggcaaaa gcaccccata ccacccaacg
     6301 ccgtccatcc atcgccttcc ccacttgaaa cactgatgtt gcgattgtac ccaaaagccc
     6361 ccacataccg tatatttcaa tccgactaca taccgtatcc gccttcctcc cgccgtctga
     6421 aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ctgcagacag tacaaatagt
     6481 acggaaccga ttcactcggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     6541 gaggcaacgc cgtactggtt tttgttaatc cactataaat cgttcaaata aacaggaata
     6601 taacttcaga caaacaactt accgcccgat ttgtgctatc gttttcgcac aacttaaaaa
     6661 aacctgacaa ttttgtactt ttattacaga gaaaggcttt acaaatggac ggctggacac
     6721 agacgctgtc cgcgcaaacc ctgttgggca tttcggcggc ggcaatcatc ctcattctga
     6781 ttttaatcgt caaattccgc atccacgcgc tgctgacact ggtcatcgtc agcctgctga
     6841 cggctttggc aaccggtttg cccacaggca gcattgtcaa cgacgtactg gtcaaaaact
     6901 tcggcggcac gctcggcggc gtggcgcttc tggtcggcct gggcgcgatg ctcggacgtt
     6961 tggtcgaaac atccggcggc gcacagtcgc tggcggacgc gctgatccgg atgttcggcg
     7021 aaaaacgcgc accgttcgcg ctgggcgttg cctcgctgat tttcggcttc ccgattttct
     7081 tcgatgccgg actaatcgtc atgctgccca tcgtgttcgc caccgcacgg cgcatgaaac
     7141 aggacgtact gcccttcgcg cttgcctcca tcggcgcatt ttccgtcatg cacgtcttcc
     7201 tgccgcccca tccgggcccg attgccgctt ccgaatttta cggcgcgaac atcggccaag
     7261 ttttgatttt gggtctgccg accgccttca tcacatggta tttcagcggc tatatgctcg
     7321 gcaaagtgtt ggggcgcacc atccatgttc ccgttcccga actgctcagc ggcggcacgc
     7381 aagacaacga cctgccgaaa gaacctgcca aagcaggaac ggtcgtcgcc atcatgctga
     7441 ttcccatgct gctgattttc ctgaataccg gcgtatcggc cctcatcagc gaaaaactcg
     7501 taagtgcgga cgaaacctgg gttcagacgg caaaaataat cggttcgaca ccgatcgccc
     7561 ttctgatttc cgtattggtc gcactgtttg tcttgggacg caaacgcggc gaaagcggca
     7621 gcgcgttgga aaaaaccgtg gacggcgcac tcgcccccgt ctgttccgtg attctgatta
     7681 ccggcgcggg cggtatgttc ggcggcgttt tgcgcgcttc cggcatcggc aaggcactcg
     7741 ccgacagcat ggcggatttg ggcattcccg tccttttggg ctgtttcctt gtcgccttgg
     7801 cactgcgtat cgcgcaaggt tcggcaaccg tcgccctgac caccgccgcc gcgctgatgg
     7861 ctcctgccgt tgccgccgcc ggctttaccg actggcagct cgcctgtatc gtattggcaa
     7921 cggcggcagg ttcggtcggt tgcagccact tcaacgactc cggcttctgg ctggtcggcc
     7981 gcctcttgga catggacgta ccgaccacgc tgaaaacctg gacggtcaac caaaccctca
     8041 tcgcactcat cggctttgcc ttgtccgcac tgctgttcgc catcgtctga cagacggaaa
     8101 ggatagtaaa tgactacgca ttttgtcgtt atgggcgtat gcggctgcgg caagaccacc
     8161 gccgcgctgt ccctgcagaa acacctcggt caatgtccct atgccgaagg cgacgagttc
     8221 cacacccaag ccaaccgcga caagatgggc gcgggtattc cgctgaccga tgaagaccgc
     8281 tatccgtggt tgggcaatct gcgcgactgg atgacgcaac aggcgcaaaa cggtgcgaac
     8341 cacaccatcg taacctgttc cgccctcaaa cgcggctacc gcgacattct gcgcggagcc
     8401 gaaggcaaag ctgccttcat ccacctcagt ccgccgcaag acatcaacct cgagcgcatg
     8461 atgtcgcgca aaggacatta catgaaagca gggatgctcg attcgcaact ggaaatcctc
     8521 gaggaactgg gcgaaggcga atacggggtc aaaatcgcca accccggcac gcccgaagcg
     8581 gtcgaagccg atattctgaa ctgggttgcc tcggaaaacc tgctttgaag caatatgccg
     8641 tctgaagccc gacacaggat gggtttcaga cggcataaac atcgggaaca gaatggatta
     8701 cattgattta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga
     8761 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat ctgtactgtc
     8821 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaaatgga aaatacccgg
     8881 ctatcgtctc attttcgttt taatcagcca taaaaatgcc gtctgaaacc ctttcagacg
     8941 gcatttctgt caaacgccgg acgcactcaa tccaaactca acagcaggtt gcggaacgcg
     9001 ttcgggtctt tgataaacgt catctcgccc gcctgcggaa aatgaaagtc caacaggcgc
     9061 gacacccaaa aacggatgca gccggcacgt tgggcggtcg ggaaatacgc cttttcttcg
     9121 gcactcaagg ggcgcacgcc ctcataaccg ccgataaaca cctttttcaa cgcctcatcc
     9181 aacttattgt ccgccgtcct tgcccaatcg ttgaccgcaa tcgccaagtc atacataaaa
     9241 ttgccccggc aggcataata gaaatcgatg aaacccgata cctgaccgcc gtcaagcaac
     9301 acattgtctt taaacaaatc ggcatggatg atgcccgaag gcagatgatt gccgagattg
     9361 tcctttaacg catcgatttc ggaacacagc agcgcggcat cgtcttgcga caggacgggc
     9421 agcagccggg tgcacgcctc cgtccaccac gcatcgtaac gcgggttttc catttccaaa
     9481 gggaaatcgg cggcggcaag gtgcattttg gccagcatcg caccggtatg gaaacactgc
     9541 tccgccgtcg gcagcgcggt atccgaacct ttcaggcagg caaccaaaca agccggcttg
     9601 cccgccaaaa cggaatcaag ccggccgtct ttgcgcgcaa ccggcgcggc aaccgccacg
     9661 cccttcatac tcaaatgccg gttaagctcc agaaaaaacg gcagctcttc ctgtttcaac
     9721 acttcaaaca cggtcagcac ataacgtccc gaagtcgtcg tcagaaaata attgctgttg
     9781 gtaatcccct gcgcgatgcc ctgcagggaa acaaattccc ccaaatcgta accgctcagg
     9841 aagccgcgca tttcatcatc ggaaacactg gtatagacag acatatcaac tcacttgctc
     9901 aaaaacgggc aaacccgccg tcagaacgcc ggacggcagg caaacatata attcacatcg
     9961 gtcgaatcgc acagggcata agtttgcgac aacacatggt aagtcatacc cttcgtatcc
    10021 gccacatcca agcctgcctg acggcacatt cgcgccagct cggcaggtgc gatgaatttt
    10081 ttccagtcgt gcgtgccttt ggggacaaac ttcaacagat attccgccgc cacaatcaga
    10141 tgcaggtacg atttcgggtt tttattgatg gtggaaaaaa acaccatgcc gtccggcttg
    10201 accagcttgg cacaagcacg cacgatggcg gcgggatcgg ggacgtgttc catcatttcc
    10261 atgcacgtta ccacatcgaa cgagtgcggt tccgcctcgg caaggtcttc cacgcggata
    10321 cattcgtatt cgatatcggc gacattgttc aaagccgcgt gcaggcgggc ggtttccaac
    10381 gactgctccg ccatgtcgat gccctttaca aacgccgcgc cgcgccgtgc catactttcc
    10441 gccaagatgc cgccgccgca gcccacgtcc aaaacccgtt tgccgcacaa atccgcgtgc
    10501 ccgtcgatat aatccagccg cagcggattg atgtcgtgca aggttttgaa ctcgcccgac
    10561 ttgtcccacc atttgtcggc aatccggctg aatttggcga tttccccctc atcgacatta
    10621 tattttttgt cggacatttt ccctcccatc tgacgaaccg cccactccaa aacccaagat
    10681 tatactatgg aacacaatgc cgtctgaaag ccttttcggg cggcgcggcg caaatgcctt
    10741 acaaatcctt acactttacg gcataatggc ggctcgcttt ttctggcaga aagacaaaat
    10801 atgcccacca aaaccccttc actgttcggc ggcgcgatga ttatcgccgg cacggtcatc
    10861 ggcgcaggta tgctcgccaa cccgaccgcc acatccggcg tatggtttac cggctcgctg
    10921 gccgtgttgc tgtacacctg gttttctatg ctttccagcg gcctgatgat tttggaagtc
    10981 aacacccact atccccacgg cgcaagtttc gacacgatgg tcaaagacct gctcggacgc
    11041 ggctggaaca tcatcaacgg catcgccgtc gccttcgttt tatacctgct cacctacgcc
    11101 tacatcttcg ttggcggcga cctgaccgcc aaaggcttag gcagcgcctt gggcggcaat
    11161 gtttcactca ccgtcggaca actcgtcttc ttcggcatcc tcgccttttg cgtatgggca
    11221 tccgcacgct tggtcgaccg cttcaccagc gtcctcatcg gcggcatggt attaaccttt
    11281 atttgggcaa ccggcggcct gattgccgat gccaaaatgc ccgtcctgtt cgacacccaa
    11341 gcccccgtcg gcaccaacta ctggatttac gccgccaccg ccctgcccgt ctgccttgcg
    11401 tcattcggtt tccacggcaa cgtctccagc ctgctcaaat actttaaagg cgacgcgccc
    11461 aaagtggcta aatccatctg gacgggcaca ctgattgcgc tggtaattta cgtcctctgg
    11521 caaaccgcca tccaaggcaa cctgccgcgc aacgagttcg cccccgtgat tgccgccgaa
    11581 gggcaagtct ccgtcctcat cgaaaccctg tccaaattcg cccaaaccgg caatatggat
    11641 aaaatattgt ccctgttttc ctatatggcg atcgccacct cgtttttagg cgtaacctta
    11701 ggtctgttcg actacatcgc cgacatcttc aaatggaacg acagcgtgtc cggccgcacc
    11761 aaaaccgccg cgctgacctt cctgccgcct ctaatttcct gcctgctctt ccccaccggc
    11821 ttcgttaccg ccatcggcta cgtcggcctg gcggcaaccg tctggacagg catcatccct
    11881 gccatgctgc tctaccgctc gcgccaaaaa ttcggcgcag gcaaaaccta taaagtttac
    11941 ggcggcttgt ggctgatggt ttgggtcttc cttttcggca tcatcaacat cgccgcacag
    12001 gtattgagcc aaatggaact cgtccccgta tttaaaggat aaaggcaaaa tgccgtttga
    12061 agcccgccgg cggcttcaga cggcattgcc gcaacaaacg gcaaccgtat tccggcacac
    12121 agcgcattac cctgcccctc acgcacaaat cccgccccga caaaccggga cgcaaccata
    12181 aaggaacaat gatgaagctc aaaatagata ttgcaaccaa caacttcaaa cacggcggcg
    12241 gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac atcacaccgg
    12301 ccgtttatgc gacgaaattt gatcacggca ttcctgaata cgccatgatc gaaccccatc
    12361 ttgtcgatca acaccggacg ctgaaaaaac tacgctcatt cctcttttca agccggctcg
    12421 ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc gattacgccg
    12481 acctcctcat ctgcggcggc acacacttgg gctacctgca ccatatggcg caaaaaccga
    12541 acctgctcga ccgcctcgcc atacgccgca accgcagcaa ctacgccacc gccaaactga
    12601 ttatggcgca ttcccatatg atgcagcgcg aactggtcgg actatacggc gttcccccc
//
LOCUS       DAXIHS010000060        12483 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXIHS010000060 DAXIHS010000000
VERSION     DAXIHS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.060"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(71..487)
                     /locus_tag="ACFOF2_001462"
     CDS             complement(71..487)
                     /locus_tag="ACFOF2_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107757.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(484..675)
                     /locus_tag="ACFOF2_001463"
     CDS             complement(484..675)
                     /locus_tag="ACFOF2_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107758.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            1198..1365
                     /locus_tag="ACFOF2_001464"
     CDS             1198..1365
                     /locus_tag="ACFOF2_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107759.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1638..1823
                     /locus_tag="ACFOF2_001465"
     CDS             1638..1823
                     /locus_tag="ACFOF2_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107760.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2024..2224
                     /locus_tag="ACFOF2_001466"
     CDS             2024..2224
                     /locus_tag="ACFOF2_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107761.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2374..2964
                     /locus_tag="ACFOF2_001467"
     CDS             2374..2964
                     /locus_tag="ACFOF2_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFC7107762.1"
                     /translation="MNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSKWIQRRI
                     EEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERNDKGRQAR
                     RYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIGKLLDMN
                     PGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            2968..3183
                     /locus_tag="ACFOF2_001468"
     CDS             2968..3183
                     /locus_tag="ACFOF2_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107763.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3344..3862)
                     /locus_tag="ACFOF2_001469"
     CDS             complement(3344..3862)
                     /locus_tag="ACFOF2_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC7107764.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(3972..4139)
                     /locus_tag="ACFOF2_001470"
     CDS             complement(3972..4139)
                     /locus_tag="ACFOF2_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107765.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4132..4305)
                     /locus_tag="ACFOF2_001471"
     CDS             complement(4132..4305)
                     /locus_tag="ACFOF2_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107766.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4414..4680)
                     /locus_tag="ACFOF2_001472"
     CDS             complement(4414..4680)
                     /locus_tag="ACFOF2_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107767.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(4770..5684)
                     /locus_tag="ACFOF2_001473"
     CDS             complement(4770..5684)
                     /locus_tag="ACFOF2_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFC7107768.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            complement(5720..7696)
                     /locus_tag="ACFOF2_001474"
     CDS             complement(5720..7696)
                     /locus_tag="ACFOF2_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC7107769.1"
                     /translation="MKYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     NARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIVGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAAW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            complement(7698..8027)
                     /locus_tag="ACFOF2_001475"
     CDS             complement(7698..8027)
                     /locus_tag="ACFOF2_001475"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC7107770.1"
                     /translation="MYIIQVTVKAGIKACKVSNGFYHGGIMQANEIYKSLEEKNISAR
                     MLASALGVTNQSVSEVIRNGRGSKRIAEAIAKVIQKDLVEVFPHYKKTDCRDKKIAEL
                     KEMLGVG"
     gene            7993..8892
                     /locus_tag="ACFOF2_001476"
     CDS             7993..8892
                     /locus_tag="ACFOF2_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFC7107771.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9101..9271
                     /locus_tag="ACFOF2_001477"
     CDS             9101..9271
                     /locus_tag="ACFOF2_001477"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC7107772.1"
                     /translation="MRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDLSNTQDDIAI
                     IGRVEWYGRSID"
     gene            9481..10071
                     /locus_tag="ACFOF2_001478"
                     /pseudo
     CDS             9481..10071
                     /locus_tag="ACFOF2_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            10176..10844
                     /gene="hda"
                     /locus_tag="ACFOF2_001479"
     CDS             10176..10844
                     /gene="hda"
                     /locus_tag="ACFOF2_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC7107773.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            10841..11509
                     /locus_tag="ACFOF2_001480"
     CDS             10841..11509
                     /locus_tag="ACFOF2_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC7107774.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3362 a         3297 c         2529 g         3295 t
ORIGIN      
        1 aaaaatgccg tctgaaaacg agctttgcga gtttcgccaa aacgtttcag acgacctttt
       61 ttactacaat ctaatcactg tctttttcgc gccgttgctt ttccaaggtt tgcgctttac
      121 ctctgttgat tcctgcaaaa tatcccaaag ctaccgcccc gccgattttg acaatttcaa
      181 tagcgacgct gtccttatcg cgccataacg cagtaatcag cacggcggca atcagaaaag
      241 aaatagcaat cacggcattc agacggcgtt tgtgtacctc tttgaaaatc tcaccatgtt
      301 gctgttcacc ctgtttttgc gcttcgatgg tagccagtgc aatgcgttcg tgagattgga
      361 tttcgtcctt acggctttcc aactccttct gtttgactgc aatatcctgt ttttgaagtt
      421 ctaaaaactc ctgaagcatt ttctgctgct catcggccgg cagattctcg gatttttccc
      481 cgctcataaa tcaaccgcca ctaaggtttt gtaacggact ttgaatttgc cgtaaccccg
      541 agcccctatt ttcggcggca caaattccac ggacgcaata ttgcccgtct tacggtcttt
      601 acggacttct tccaaataat cggcagcatt gagcagccgt tcgcggactg ccgtatattc
      661 tttgacggta ttcatttttc aacctcgccc aaatagtgca aatattagca tatgggattt
      721 atttttcaag aaaatcaaat ccatataaaa caaccgtata atattttaat actatatatg
      781 gtaaatttct tctaaaaata cacactaaaa taccatatct tgataaccgc aacgccgcac
      841 aaatggcagg gcattgatgc ccgacaacaa aaaatgccgt ctgaaacagc attcgagctg
      901 ttttcagacg gcatttttct ttgattttgt tggggaaagg gttgactttt aacgtgtcca
      961 cgtttataat tcaattcatc ggcgtagtga gggccgatac gggaaaagcc ccaccgaagc
     1021 ggggctcaac tgggagaaag aagatgaata aacgcactat tcaaattctc atcttggtcg
     1081 cagttttcat gttggcaagt gctaatgcct actaaagact gaccagctgg gggcggtcgc
     1141 cgctgtcccc agtcccaatc ttacaaaata cggtttaaaa aatcaaggag tttgcctatg
     1201 gttgatgaaa aactggcaga aaaccgaaag cgttacgagc agaaacgggt gattaagaaa
     1261 gtctcgttca atgccgaaac ggaaaaagaa cttttggaat atgcccaaaa tatcgatttt
     1321 tcccaatggg ttaaatcaat catcaaagaa aaaatcaaaa aatagcttga ctaaattcta
     1381 gaaatactag ataatacgcc tatctgataa agcaaagccc cgaagcggca acttcggggg
     1441 cttatcggat agaaaggagg cttgtggaaa tgacaaaact ggcgaaagtt gtttttttca
     1501 tcattctgct tttaagctgt agtccggctt actaagcaag ttgattaaac acaaggggtg
     1561 gcagaacacc gcgccccttt cgccaacctt tctgcaagcc attttactac aacgatttta
     1621 aaaatcaagg attgcctatg cctgatgaaa aaacgctaaa agcaaaacaa gctgaatatc
     1681 agcgaaagta tgaccaaaag cggctgatga aaacagtttc tttccatttg gaaagagaga
     1741 aagagttggt ggattttatg caaaaagaaa ttcccgactt ttcaaactgg gttaaatcag
     1801 tcatgaagga aaaaatgaaa taagaaaatt ccgccgatgg gaaaacatcg gcggaacagg
     1861 tggaaagcag agcagcaact ctctttatgt agggcatgaa aaaaggccgt ctgaaacagg
     1921 ttttaaaccc catttcagac ggctttttgt tgtaatacat cttgattaaa atggttttta
     1981 gtattaccat aatcgtgttt taaaacctat ttaaaggaag attatggact cgattaaaaa
     2041 ggctcaagaa aaatacgcac tcaagcgagt agtgaaaaca gtatcgttca attccgaaaa
     2101 agaacttgat ttaattaaat tttctgaaaa tttagatttt tcaaactggg taaaatcgaa
     2161 aattagagga gatatggaat cggaaaaatt caaaaaaaaa gcaaaaaaga atattgacaa
     2221 atagaattac cataatttaa aatttccata tcttgtgcaa cacaagatat ggaaatcccc
     2281 gataagtgac agcttatcgg ggatagtgga aaagcaacgt tagagcgttc ttttccttgt
     2341 catctcaacc ttgaaatgaa agataatttt accatgaata cccaactcat cccaactgtt
     2401 tcaggtcaat tggacaatca aacacaagca ctggttgatg ctcatgacct gcataagttc
     2461 ttaggtgtgg aaacaccatt ttctaaatgg attcagcgtc gcattgaaga atacggattt
     2521 acacaagccc ttgattttat cggcgtggac aaaattgtcc gcaccgaagc aggtttcttt
     2581 ggacagcgtg ataaaactgt tcaggggtat tatttatccc tcgacatggc aaaagagctg
     2641 tgcatggtgg agcgcaacga caaaggacgc caagcccgcc gatattttat cgaaatggag
     2701 aaacaggcaa aagccctgcc tgatgccgtg ctataccgca tcgatgcatt ggaagacgcc
     2761 tatttccaag ccgcgcccga aatgctcgcg ctgctgcgct accgcagtat gggcttgaac
     2821 ctgaccgaaa taggcaaact cttggacatg aaccccggcg cggtttccta ccgtctcaaa
     2881 aaactcaacg atttgggttt cttggaatat gtccccaagc ccaaaatggt ggtgcaacaa
     2941 cagtctttgg gattggaggg ttaagccatg aataccacct ataccctgac tttcgaccaa
     3001 gacagcctca aagccctgaa tctgctggtg ttcacactca accatctgaa agttttggag
     3061 atggacatgg aaggaatcga agacggtttg tctgccgtga ttgaaaccgc ctccgagaaa
     3121 gccgacaagc tgtccgccgc tttctacaac gccgtgtacg agcaggaaga gaaagcagcc
     3181 tgaatctagg gcatgaaaaa aggccgtctg aaacaggttt taaaccccgt ttcagacgac
     3241 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
     3301 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
     3361 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccggc acttggccgt
     3421 ctgaaaaacg ctctttttga ttcaggatgg cgtctttgtc gatttccttt ttagtgcgga
     3481 caaactcggc aaaggcggat ttttccgaga gccacgccaa gacggcggcc acgcccgtta
     3541 ccttgacgga tggcggacgg atgcgccatt taatcaggcc ggtagtaaag tccacggttt
     3601 tggttttacc gttttccgtc agctcgtcct tatgtgcctc gcagtatgcg gccacacgtt
     3661 cggtcaggct catgatttcg gcacacatcg gcgcggcttg ggcggcatat tcttcttcga
     3721 tggccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
     3781 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
     3841 gtttgattcg ggttttagcc attttctctt cctttcaggt tgggtttaac atttcggctt
     3901 actcaagcct tgttcgcgtt cgtgcttggc aagtgcttca actttcgcgc ggtttctcaa
     3961 aaacttaaac atcacacttc ctcccataaa gttatcgcct gagccaaagt ttccgcctcg
     4021 gcagtcttcc acatcccgtc cggcgcacgc gctgcaatca caaagccttc gccgtccttc
     4081 ttcatgacca tcagctcgcc acggtcttcg agccattcga ttaaatcttt ttcattcatc
     4141 tttgtttcct tccgttttca gacgaccttt gccgtcctta tcttcaaact gcgcctgata
     4201 ttcggcgact tcctgctcgc ggtttcgctt caccataaac ttcgtcgcac gcctgcggtg
     4261 ttgtccccaa gcctgccaat ccgtattacg tcgtccgaaa ctcatttcac acatcctttc
     4321 acaatcgcct tatcgccata tttcgcgcgg atttccttta ccgcccgtgc caaagcctct
     4381 tttttcgccg cagggctcaa taaaattggc ttatcactca taaacaatcc ctttcatttt
     4441 ttcctcgacg ctcattgact cgtattgccc ttcccatacc ttgacctcca aatccgcctg
     4501 tctttccatc gcctcaacct tcgtcggctc tttcgcaacc ggttcgggtt ctttcgcgca
     4561 ggaatgcaac gccattcccg ccacaaaaca ccacacgccc accgtcaaac caaccggcac
     4621 ccaacgccaa aaagagcgcg ccacaaacat cttccaatca actttcttca aaacttgcat
     4681 tttgcgtttt ccttttaaaa caacaactta ttaaaatcat agggtaaaaa aatatatagc
     4741 cctgtcagag acttaccgtt tcagacggcc tatcggataa tcagggagct gtatttctta
     4801 acgataccgg cctgcatctt gataccgttc ttgttcgccg tgcgtaccgc gccgcgcatc
     4861 aatttactca tccggcgcgt attgccgtta ctatgtttaa ccagttccaa gagcgtttct
     4921 tcgtccgcat caggcaaagc cgctttcgca atctcaaaga gttcgtcatc cggcaaagat
     4981 tcgcccaaat tcagcgcaac agacacgcgg ctataaagtt gcaccagctc gccatgctta
     5041 ccgcgcaaat tcgcaaccag tcggggcata ccgctcaaca ccaaaccgca gcccgtttca
     5101 tcatgcagtc ggcggataat ctcaagggcg cgcaagggca ggttttccgc ttcatcgacc
     5161 acaatcagac ggcccgaatc gcgcaggcgg tcagatacag actcaaacaa atcattcagg
     5221 ctgcccatcg ccgatacctt cgccgccgtc gccaacttgc gcatcagtac caaagccgta
     5281 aagctcggat tagcctcaat caagatagcc gcagggtttt tctcgcagta gtttttgacc
     5341 gactgcgtct tgcccaaacc cgcctgaccg tagatcacca ccgtttcgcc gccttcgtgc
     5401 gcatcgcgca tcacttccgc gattcggcgt gtcgtcttag tcgatacaaa tcccaacacc
     5461 agctcttcac gtcgcgcctt actgtcctcc atctccaaaa acgcctcgat tttcgtctcg
     5521 atggtttcat aattgccgcc tttatcggca taagtgccgt tcagatacat actgatggag
     5581 gccggcgaag tatcgatacc gcgtgccagt tgggtttggt tcattcccga tttttcttta
     5641 aattcaacca gtttttgttg caatgcttga ttaatttgtt tcattttttt agtcctttta
     5701 aaagaggttt aaaaccgttt taacttctat ccgtctcaaa tagcacaaaa tcgtccgtgc
     5761 ccgttttcgg caataccgca tactccgcct cgatgacgtt tccgcccaaa tgtcccagct
     5821 cgtcccaagc tgccgcctgt tccagtgccg gattgacttc cgcatttgcg agcttgattg
     5881 cattttccgc ccgcttgatt ttgccttttc ggcggttttc cgccagttgg tcgatacgcg
     5941 ccgtcgggaa agcctcgcgg gtattgccgt cggcctgcgc cttagtgata aacttgccgt
     6001 ccatatcaaa cacattgacc accgacgcat cgctcaaatc gtagctgacc cgtacctcgt
     6061 ccttgtgata ctccgccagc tcggttgaga aataagagtt gttgaacaaa tccagccaac
     6121 cgcgctgtac ctttcgcacc tcttgcggca taaacatcgt cgccagctct tgcgccgaca
     6181 acatatccgg cgcgataccg tcctgttcca gcctcatttc ccgataagcc ttcggcgtat
     6241 aatgcccgcc gtcaggatgt cggggcagct cgccgtgcgg gcggttgttg tattcgtcga
     6301 tacacttgac cacatccgcg ataaaacgcg accagctcgg cagttttttc aaatatttct
     6361 gttgttcctc cgttaaatcc ttgccttttt ccaaagcatt aaacgcactt tccatcttgc
     6421 ggtacatcag gttcttcgta ctgctgtcca ttcctgcgcc cgcaaacgtc tcatactggc
     6481 gcgccatctc aatcagattg tctttccacc atcgctcaat gatgccgcgt ccttgcgggt
     6541 tgcccacgat acccgtttca tgccggatac ccaatcggga cgtaatacct gtgatttcat
     6601 ggtctatcgt cttgccggtt tggccgccgc cgttatccga gtaatagata atcggcaaac
     6661 caaaatgctt gaccccgata cgcagagcgt ccgataccgc cacacaactt tcagcaagag
     6721 agaccgaaaa cccgaccaca aaccgcgtac aaccatcaat aatcaccgtc acttccggct
     6781 taaacggcct gccgtgtaca gggtgcgcca ccttcgcctt aaagctgtgg ccgtcgccga
     6841 tccaaacatc gttcggcttc aaagcccccc aatcacgttt cacataaggc agcagcgatt
     6901 tataagccgc ccccgttttc ctgccgcgct cctgcataat cagcggcagc ttgtcccaaa
     6961 cgcgacgcac catactcaaa ttaggcacat cattgaccgg catattttcc gcttcagccc
     7021 actgcacaaa tcggcggtaa ctgtgcgcca gcttcggagc tgaagggatg ttgtgaaact
     7081 gcataaacat cggcaaccaa ccgtagctct caatcggctt gaccgccttc gtcgtcttcg
     7141 gagccaaagc aaccaaccgc tccgtcgcgt tttccgcttt caaataagca gaaatccagc
     7201 cgtctaaagt acgttcgcca acctttgccg tccggctgcg gtcattggcc ttttccaagt
     7261 tcccaagcgt gaccgcgtcc aatttacttt ctgccagcaa gcccaaaaac tgagccaccg
     7321 cagcctttgc agagcaaccg tattggtatt taatccccaa taccgccgcc accaccgcac
     7381 atcgcgcatt cgccaccgac cgttgtttct cgttcaacag cttggccgct tcagccagtg
     7441 cctgagccga catcgccgtc cccggtctga cttggggcag cattttcggc atcttctccg
     7501 ccagctcgtc cgactgccgt ttcatgatgg ctgctcggat ttcggcaggg agggcatatt
     7561 caacaccacc acccttacct tgacgccttc tcgtttgcca accatccctt tgcgcttttt
     7621 ttctcagccc tattactgtt cttggcatat ttggcaaccc aaaattaagc aggcttttga
     7681 ggtccatata tttcatttca acccactccg agcatctctt tcaattcagc tatctttttg
     7741 tctcgacaat ctgttttctt gtaatgcgga aaaacttcaa ctaaatcttt ttgaatcact
     7801 ttcgcgattg cttccgcaat tcttttgctt cctcgtccat ttcttatgac ctcagaaaca
     7861 gattggttag ttacccctaa agccgaagcc aacattcttg ccgatatatt tttttcttct
     7921 aaggatttat agatttcgtt cgcttgcata atgcctccat gatagaatcc attacttacc
     7981 ttacaggctt taatgcctgc cttaactgtt acttggatga tatacacaaa atatttggta
     8041 taccaaatat atttggtaat attttgcgat gtttttatgg aacgcattga tttagcattg
     8101 aatttagttg aagagagaag ccgacttgga tactctcgaa agaattttgc tgagcaaact
     8161 ggatgcagtg cagaaagttt gagactgtat gaaagcgggc aggtcaacat tcctgcggac
     8221 tttttacttg ctgctgtcca attaggggtg gatattcaat ttgtctttac aggcattcat
     8281 tcggcaaatt taaatgctgt ttcagacgac cttaataaaa aattggacga cgctattggc
     8341 aaatcaaacg ttgagaatca gattaacggc aacgtcagta acgctgttat cgcctcatct
     8401 ggttcggttg ttcatcaaat taatacccaa aatcacgtca tcagaacccg tgtcgattca
     8461 aaacccggca aggaacatat aacgcttgag caagcttcaa agctgcaaca acttgttaaa
     8521 caagttgccg cagcagaaga gattgccaaa cggtcgccaa aatcaatccg tgcaatttgg
     8581 gctagtctga atgctcactg taaagttcca agttacaaac taatcgcatt gagcgattac
     8641 gacaaagcag aaacttatct cagaaaatgg ctgggtaggt tgagcaatac agcaacatca
     8701 aagaacaacg atcccgactg gcgaaaaaag aaatacgctt atatcaaatt aaatgtaaaa
     8761 cagcttgagc ttgaagattg gttgaaaagt tacctcgaaa aaaatttctc agtagaaagc
     8821 ctgactgaat taagtgatga agatttgcaa aaaacatatg cagcagtatc gactaaaaaa
     8881 cggaaaaaat aggcttaaac atgaaactcc gcctttaaat tcaagtttca tgtttagctt
     8941 atggtcttca gatgctttgt ttttttagat gtatgatatt aaaagatttt ttcaaacatg
     9001 gaactattat acaagttcca tgttcagttt ctagttaaag ccttttataa acattctaat
     9061 caaccacgcc gaaaccgagc cgcgcgacgg cctatatgtc ttgcgtatag gcaatgacct
     9121 ctttgtcaaa cgtgtgcaac gtataccggg caaactgctt gtaacgtctg aaaatccacg
     9181 ttacgcaccg tttgaaatag atttgagtaa tacccaagac gacatcgcca tcataggccg
     9241 tgttgagtgg tatggccgga gtattgatta aatccatttt aaaaacctct taaaacagtt
     9301 ttaaaaattc cctaatctcg tcagctttaa acagaaaacc gcgcattccg gcgcggtttt
     9361 gtgaaaaagc tggcgtaact tttccggata caaaaaaacg ccgaaatcca cgtcttccga
     9421 agatttcagc gtttttttac tctatttgtt ccttgtgcaa aaactaacag tcccccacag
     9481 gaaaacgtcg ccctgttccg ccacaacggc gaagttttat tggacggcat caacatcaaa
     9541 ctcaaaagcg gcgattcgct gctgatacgc ggcccgagcg gttgcggcaa aacctcgctg
     9601 ctgcgcgcgc tggctgggct ttggccgttc ggcagcagcg gcaaagtcag ccgtccgccg
     9661 catcaagaca tcctcttcct gccgcaacgc ccgtacacgg cacagggcag cctgcgcgac
     9721 gcggtttgtt atccggacat cgacaaacag catcccgagt tagccgaagc catgaacacc
     9781 tgccgcttgg gttatctgat tgacaaattg gacaaaacag acgactggca gcacaaactc
     9841 tccccgggcg aactgcaacg cgtcgccttc gtccgcgccc tgctttccaa gcccaaaatc
     9901 gtcctgctcg acgaagccac cgccgctttg gacgaaccga ccgaggccct gctctaccgc
     9961 gcactgaaac aaaaactgcc cgacagcatc atcatcagca tcggccaccg cagcacgctc
    10021 aacgcgttcc acaatatgca cctcgatgtc ggcaacgttg cctgcggttg aacgatgccg
    10081 tctgaagccg ttttcagacg gcatttttca aattgataaa gcacgcgaaa acaacgtaca
    10141 ataaacccgt ttaccacccc tcggaatgcc gaaccgtgaa ccagcttatt ttcgactttg
    10201 ccgcacacga ctatccgagt ttcgacaaat tcctcggcac ggaaaacgcg gaactggtct
    10261 atgtcctccg acacaaacac ggacagttta tctatgtctg gggtgaagaa ggtgcgggca
    10321 aaagccatct tttgcaggcg tgggtcgcac aggcgctcga agccggcaaa aacgccgcct
    10381 atatcgatgc cgcctccatg ccgctgaccg atgccgcgtt cgaggcggaa tacctcgccg
    10441 tcgatcaagt cgaaaagctg ggcaacgaag aacaagccct gcttttttcc atcttcaacc
    10501 gcttccgcaa cagcggcaaa ggctttttgc tgctcggttc ggaatacacg ccccagcagc
    10561 tcgtcatccg cgaagatttg cggacgcgta tggcgtactg cctcgtttac gaagtcaagc
    10621 ccttaaccga ccaagaaaaa atcgacgcgc tcgccagtat ggcggcggca cgccaagtaa
    10681 ccgtcgattc cgaaattttc gaatacctgc tcaaacactg gcggcgcgat atggacagcc
    10741 tgatgatgat gctcgacacg ctggacaact acgccgtcac aatgggcaaa cgcatcaccc
    10801 tgccgctttt gcgccagctt ttgaaacaac aggaaaccca atgaaaaacc tcgccatctt
    10861 cgacctcgac aacaccctca tcaacaccga ttccgaccac gcctggccgc aatacctcat
    10921 caaaaaaggc ttggttgacg ctgccgaaac cgaagcgcaa aacgaaaaat tctaccgcga
    10981 ctaccaaaac ggctgcctcg acatcgacgc attcctcaaa ttccacctcg ccccgctcgc
    11041 tcgttacagc aaagaagagc tggcggaatt tcaccgcgaa tttatggcgg aatacatcat
    11101 cccccacatc tcgcctatgc agcgtatgct ggtgcagagc caccaaatgg caggcgacga
    11161 aacccttgtg atttcctcaa ccaacgagtt catcatcacc cccgtctgcc accttttcgg
    11221 catcaccaac atcatcggca cacaactcga aaccggttct gacggccgct acaccggcaa
    11281 ttacatcggc acgcccagcc tcaaagaagg caaaatcacc cgcctgaacc aatggcttgc
    11341 cgaacgcggc gaaacgctcc aaagctacgg caaaacctat ttttacagcg actccaaaaa
    11401 cgacttgccg ctgctgcgcc ttgtcagcga acccgtcgcc gtcaaccccg atgccgagct
    11461 ggaaaaagaa gccaaagaaa aaggctggcc ggttttgaat ttcaaatgac gcaatgccgt
    11521 ctgaagcccg attcggcgtt cagacggcat tgaggctgaa atccaaaaaa ctcaaagcca
    11581 caggaattta tccggaaaaa caaaaaacac aggaattcat cgggaaaaac aacaatcttt
    11641 ccaccgtcat tcccacgaaa gtgggaatct tgatccgtcc gcacggaaac ttatatttcg
    11701 tcatttgcac ccgacataat tcaatacttt attttgcaat aaattaacgg atgctgacaa
    11761 attagattgg attaattacc ctattatcca tattgggtta tcgggttgct aattgagcag
    11821 agcgtagcaa accataaagt gagtgtttcc acccactctg catttactat gaagtggtta
    11881 ttaaatatga taatgagacc tattaaattt agtatggtaa atacgttatt atttattgtt
    11941 atatgtagtt cattttttga tctgctcgtt caattatgta caattttatt tcatagccaa
    12001 aaaatatact ttattacatt atttttatta tttattttta attttgttac aaaatctatc
    12061 tatatggcaa ttatttatcc tattttatat ttttttacga taaaaaaata ttatccttac
    12121 tctaggaaag tgataattct attatcatta gcattatcta tatattttag ttttatggac
    12181 ttttactttt tttccatata ttcagataac cttagctatg aaacggagcc tttacattta
    12241 tacatcccta ttattattaa ttttttctca cttttagttt ctaattttat tttatctttt
    12301 atcaacaagt aaatgtatat acaaactaca atccaaaaga tcctaagcaa accaccaatc
    12361 ccacaccacc cttaccggtt caaatccgca caaaaaccca catcccgtca ttcccacgaa
    12421 agtgggaatc taggacgtat agtggattaa caaaaatcag gacaaggcga ccaagccgca
    12481 gac
//
LOCUS       DAXIHS010000061        12183 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXIHS010000061 DAXIHS010000000
VERSION     DAXIHS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.061"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(260..1189)
                     /locus_tag="ACFOF2_001481"
     CDS             complement(260..1189)
                     /locus_tag="ACFOF2_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC7107775.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1320..2069
                     /locus_tag="ACFOF2_001482"
     CDS             1320..2069
                     /locus_tag="ACFOF2_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC7107776.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2192..2773
                     /gene="petA"
                     /locus_tag="ACFOF2_001483"
     CDS             2192..2773
                     /gene="petA"
                     /locus_tag="ACFOF2_001483"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC7107777.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2792..4141
                     /locus_tag="ACFOF2_001484"
     CDS             2792..4141
                     /locus_tag="ACFOF2_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC7107778.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4156..4944
                     /locus_tag="ACFOF2_001485"
     CDS             4156..4944
                     /locus_tag="ACFOF2_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC7107779.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5283..7067
                     /gene="rmuC"
                     /locus_tag="ACFOF2_001486"
     CDS             5283..7067
                     /gene="rmuC"
                     /locus_tag="ACFOF2_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC7107780.1"
                     /translation="MELMTVLLPLAALLSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAYLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAAAVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTEHANETTALSESLEYAAEDEAV"
     gene            complement(7274..7906)
                     /locus_tag="ACFOF2_001487"
     CDS             complement(7274..7906)
                     /locus_tag="ACFOF2_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC7107781.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTRKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFSPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7998..8822
                     /locus_tag="ACFOF2_001488"
     CDS             7998..8822
                     /locus_tag="ACFOF2_001488"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC7107782.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8891..9454
                     /locus_tag="ACFOF2_001489"
     CDS             8891..9454
                     /locus_tag="ACFOF2_001489"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC7107783.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9535..9972
                     /locus_tag="ACFOF2_001490"
     CDS             9535..9972
                     /locus_tag="ACFOF2_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC7107784.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            10041..10310
                     /locus_tag="ACFOF2_001491"
     CDS             10041..10310
                     /locus_tag="ACFOF2_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC7107785.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10310..12085
                     /gene="ptsP"
                     /locus_tag="ACFOF2_001492"
     CDS             10310..12085
                     /gene="ptsP"
                     /locus_tag="ACFOF2_001492"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC7107786.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         2984 a         3043 c         3429 g         2727 t
ORIGIN      
        1 tatttggggc gaaaacagct gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcaga ttgggcaaaa atcaaaaaca gtatagtgga
      121 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg
      181 aggcaacgct gtactggttt ttgttaatcc actatacatg aaaaaataga aaaactgccg
      241 ggtatggttg aataaggggt cagaccggtt ccaattcgct cagtccgggc aaatctgcaa
      301 aaccgcgttc gcgtatgatt tggcaaaaat tgttcagata gctcttgtcc gtatcttcgg
      361 tacggatggc ggcatacagt ttgctttgca atccgtcggc agtaatttgg cggtggacga
      421 catagccttt ttcaaggtag ggcatgactg tccaataggg aagggcggca atgccacgtc
      481 tgctggcaac cagttggata atggcgatgg tcagttcgct gtgtcggcgc ggcgggttga
      541 tgtttttcgg aatcaggatt tttttgggca aatccagcat ctcgtcggga acgggataag
      601 taatcagggt ttccccgata aagtcttccg ccgtccaaac gtttttggcg gcaagcggat
      661 ggtctggtgc gcaaatgccg accatttcgt aggcaaacag cggttggaaa gaaatatcgt
      721 tttgtttttc cgcttcggaa acaatggcaa ggtcggcacg gtgttgcagc agcagtccga
      781 cgggatccgc ttggaatccc gatacgatat ccaattcgac ttggggccac atcgggcgga
      841 attcgcccat ggcgggcatc agccagtcga aacaggtatg gcattcgacg gcaatccgca
      901 gctctcccgc ctctccttcc gtgattcgcg ccaaatccca ttctgcaaca gcaacttgag
      961 gtataagttc gtgggcgagg cgcagcagcc tttcgcccac cggggtaaag cgcaagggcg
     1021 tggatttgcg ttcgaacagc ggcgtgccgt agtggttttc gagcatacgg atctggtggg
     1081 aaagagcgga ttgggtaagg aaaacccgtt tggcggcaag ggagacgctg ccggtttctt
     1141 caagtgccag cagggtcttg aggtggcgca gttcgataat ggaatccatg gggcttcaga
     1201 cggcatattg aaccggcgca tattaaaata attcatatgc gggtgcaaat cggaaaaatg
     1261 tgaattccgg acattttgcg attagaatgc ccgcttgttt aaagcgatta ggaaaaaaga
     1321 tggtattgcg cagggatttt ctgacttggt gtaatgaaac attgcagaca gcgttgttta
     1381 aagattacgc ccctaacggt ttgcaggttg aagggaggga atatataggg aaaatcgtta
     1441 cgtcggtaac ggcaagcagg gcagcgattg attttgctgt ggagcagaag gcagatttgc
     1501 ttttggtaca tcacggtatg ttttggaaaa acgagttgcc gaccgttact ggttggaaaa
     1561 aagaacggat tgccgcactg ttacggcacg acatcaatat ggcaggctac catctgcccc
     1621 tggatgcaca tcccacactg ggcaacaatg cccaactcgc cgacagattg ggttttgcga
     1681 cagaaaaacg gttcggcgaa caaaacctgc tcaactcggg cagcctgaaa caagccaaga
     1741 cactcggcgc attggcggcg catattgaaa cagttttgca acgtaagcct gtcgtcatcg
     1801 gcaaacccga acgcgaaatc cgacgggttg catggtgcag cggcggggcg caggggtttt
     1861 ttcagacggc aatagacgaa ggtgtcgatc tgtatctgac gggggaaatc tctgaagccc
     1921 aataccatct cgccaatgaa acgggtacgg ctttcatttc ggcagggcat cacgcgacgg
     1981 aacgttacgg cgtacgcgca ctggcagaat cggcggcaga ggttttcggg ttggaagtgt
     2041 gccattttga cgaaaacaat ccggcttgag tcttgagaaa tatcataaaa ctttacctta
     2101 ttttaaataa tgctttgagt atcactgcaa actgggtaaa attgcaacgt ttaatggctc
     2161 ggtattccca aaatattagg acgactgaat aatggataat caagaaatca acaacggccg
     2221 ccgccgtttc ctgacactcg cgacctgcgg cgcgggcgga gtggcagcat tgggtgtggc
     2281 aacgccgttt gtggccagtt ttttcccttc ggaaaaagcc aaggccgccg gtgctgccgt
     2341 cgaggtggat gtcagtaaaa tcgaagcggg tcagctgctg accgccgagt ggcaaggcaa
     2401 accgatttgg gtgctcaacc gtacagatca gcagcttaaa gacctgaaag gcctgaacgg
     2461 cgaacttacc gatcccaatt ccgatgcgga acagcagccg gagtatgcta aaaacgagac
     2521 ccgttcgatt aagccgaaca tccttgtcgc catcggtatc tgcacccatt tgggctgctc
     2581 gcccaccttc cgtcccgaca ttgcccctgc cgatttgggt gcagactgga aaggcggctt
     2641 cttctgcccc tgccacggtt cgaaattcga cttggccggc cgcgtatata aaggtgttcc
     2701 tgccccgacc aacctggttg tcccgccata taaatacttg agcgacacaa ctatcttggt
     2761 gggcgaagac taagaataag gaacgataat tatggcaaac caaaccaata gcaaagcaaa
     2821 agcattgtta ggctggatgg acgctcgttt ccccctgtct aaaatgtgga atgagcattt
     2881 ggctcaatac tacgcaccga aaaactttaa tttttggtac tacttcggct ctttggccct
     2941 gcttgtcctc gtgattcaaa tcgtcagcgg cattttcctg accatgaact acaaacccga
     3001 cggcaacctg aacgcctacc atctgcctgc tgcctttact gcagtcgagt acatcatgcg
     3061 cgacgtgtcc ggcggttgga ttatccgcta catgcattcc accggcgcat ctttcttctt
     3121 cattgttgtt tatctgcaca tgttccgtgg tttgatttac ggttcgtaca aaaaaccccg
     3181 tgagttggtg tggattttcg gttctctgat tttcttggca ttgatggcgg aagcctttat
     3241 gggctacctg ctgccttggg gtcagatgtc cttttggggt gcgcaggtaa ttattaacct
     3301 gttctccgcc atccctgtta tcggtccgga tttgtccact tggattcgcg gcgacttcaa
     3361 cgtttccgac gttaccctga accgcttctt tgccctgcac gttatcgcgg tacctttggt
     3421 attgctcggc ttggttgtgg cgcacatcat cgccctgcac gaagtgggtt ctaacaaccc
     3481 tgacggcgtt gaaatcaaga aaaacaaaga cgaaaacggt atcccgcgcg acggtatccc
     3541 attccatcct tactataccg ttaaagacat cttgggtgtg gttgtattcc tgattgtctt
     3601 ctgtgccgta ttgttcttcg caccggaagg cggcggctac ttcctcgaag cgccgaactt
     3661 cgatgcggca aatgcgctga aaacacctcc gcacattgcg ccggtatggt atttcacacc
     3721 gttctacgcc attttgcgtg cgattccctc gttcttggga acgcaggttt ggggtgtaat
     3781 cggtatgggc gcggcggtcg tgttgattgc cctgctgcct tggctggata ggggcgaggt
     3841 gaaatccgtc cgttaccgcg gcccgatttt caaaaccgca ttggtcttgt tcatcatttc
     3901 ctttatcggt ttgggtattt tgggcgcgat ggtggcaacc gatacgcgta ctttggtcgc
     3961 gcgcatcctg tccttcgtct atttcgcatt cttcctgggt atgccgttct ataccaagct
     4021 ggataaaaac aaaccggttc ccgaacgcgt aaccatgagc accgccaaac aaaaaatcat
     4081 gttctttgtt tacgtcggca ttaccgccgt gggtgcttac ctgtttgcaa ccaacatctg
     4141 atgagggcag tcaaaatgaa acaagctatg aaaaattggt ttgctgcctt gctgttggca
     4201 gtacccatgg gtgcggcatt tgcttcgggc ggtcatgcac actatgagaa agtcgatatc
     4261 gatttgcgcg accaagtcag cctgcagcgc ggcgcacaaa tctttaccaa ctattgcctg
     4321 tcctgccact cggcaagcgg tatgcgtttc aaccgtttga aagacatcgg tttgactgac
     4381 gaagaaatca agaaaaacct gatgtttacc accgataatg tcggcgatgt catgcattcg
     4441 gcgatgaacc cgaaagatgc ggcaaaatgg tttggtgctg ctccgcccga tttgacattg
     4501 attgcgcgtt ccaaaggtgc agactacctt tacgcttata tgcgcggctt ctataaagat
     4561 ccgacccgtc cgagcggctg gaacaatact gtattcgata aagtcggtat gccccacccg
     4621 ttgtgggaac agcaaggcgt tcaagccgtt gagttggatg ccaaaggtca gccggttatg
     4681 gtaaaagacg aacacggcga gatgaagcct aagctgtatt gggaatctac cggtttgcac
     4741 agccgccgtc tgcctaacgg caaagtgatc caaaaagagt acgacgcata tgtacgcgat
     4801 ttggtcaatt accttgtgta catgggcgaa cctgcacaac tgcaacgcaa acgtataggc
     4861 tatgtcgtga tgattttcct atttgcggtt atgctgcctt tggcgtattt cctgaaaaaa
     4921 gaatattgga aagacgtaca ctaagcgttt ggaacaaaag ggcaaatcct ttagggtttg
     4981 cccttttttc attttgcctg ccgtttgaaa agcctgaatc cgtatgccgt ctgaacatag
     5041 ctgcaacatt tcagacggca tcattctaaa aatgtcagac atcgggaact taatcagggt
     5101 ttgtggcaga ttgatattga attaggaaaa ggtctgaaac cgcaagcagc atggtaaggc
     5161 gatgtcgaac aggtttaaag ccaatccgct atattttttg gagctatgtc gaacaaacag
     5221 gtagaatcgg aataagcggc ggttttgtcc ggtgtgaaaa tgtttgaata ttaggataat
     5281 tgatggaact gatgactgtt ttgctgcctt tggcggcgtt gctgtcaggc gtgttgttta
     5341 catggttact gatgaagagc cggtttcaag gtgtattagc cgtattgaac gcgcagttgg
     5401 cggaaaaggc ggcaagatgt gattttgttg aacaggcaca cgcagaaatt gcatcggaat
     5461 tggccgtttt ggatgggaaa tacgagcatt tgcaggacga aaattatgct ttgggcaacc
     5521 gtttttccgc agccgaaaag cagattgctt atttgcagga aaaagaggcg gaatcggttc
     5581 ggctgaagca gtcgtatatc gagttgcagg aaaaggcaca gggtttggcg gttgaaaacg
     5641 aacgtttggc aacgcagctc ggacaggaac ggaaggcgtt tgccgaccaa tatgccttgg
     5701 aacgccaaat ccgccaaaga atcgaaaccg atttggaaga aagccgccaa actgtccgcg
     5761 acgtgcaaaa cgacctttcc gatgtcggca accgttttgc cgcagccgaa aaacagattg
     5821 cccatttgca ggaaaaagag gcggaagcgg agcggttgag gcagtcgcat accgagttgc
     5881 aggaaaaggc acagggtttg gcggttgaaa acgaacgttt ggcaacgcaa atcgaacagg
     5941 aacgccttgc ttctgaagag aagctgtcct tgctgggcga ggcgcgcaaa agtttgagcg
     6001 atcagtttca aaatcttgcc aacacgattt tggaagaaaa aagccgccgt tttaccgagc
     6061 agaaccgcga gcagctccat caggttttga acccgttgaa cgaacgcatc cacggtttcg
     6121 gcgagttggt caagcaaacc tatgataaag aatcgcgcga gcgactgacg ttggaaaacg
     6181 aattgaaacg gcttcagggg ctgaacgcgc agctgcacag cgaggcaaag gcactgacca
     6241 acgcgctgac cggtacgcag aacaaggttc agggtaattg gggcgagatg attctggaaa
     6301 cggttttgga aaattccggc cttcagaaag gacgggaata tgtggtgcag gcggcggctg
     6361 tccgcaaaga agaagacggc ggcacgcgcc gcctgcagcc cgacgttttg gtcaacctgc
     6421 ccgacaacaa gcagattgtg attgattcca aggtttcgct gacggcttat gtgcgctata
     6481 cgcaggcggc ggatgcggat acggcggcgc gcgaactggc ggcacacgtt gccagcatac
     6541 gcgcgcatat gaaaggcttg tcgctgaagg attacaccga tttggaaggt gtgaacactt
     6601 tggatttcgt ctttatgttt atccccgtcg aaccggccta cctgttggcg ttgcagaatg
     6661 acgcgggctt gttccaagag tgtttcgaca aacggattat gctggtcggc cccagtacgc
     6721 tgctggcgac tttgcggacg gtggcgaata tttggcgcaa cgaacagcaa aatcagaacg
     6781 cactggcgat tgcggacgaa ggcggcaagc tgtatgacaa gtttgtcggc ttcgtacaga
     6841 cgctcgaaag cgtcggcaaa ggcatcgatc aggcgcaaag cagctttcag acggcattca
     6901 agcagcttgc cgagggacgc gggaatctgg ttggccgcgc cgagaaactg cgtctgttgg
     6961 gtgtgaaggc aaacaagcag cttgcacgcg atttgaccga acatgccaat gaaacaacgg
     7021 cgttgtcgga atctttggaa tatgcggcag aagatgaagc agtctgactt gtgcggaaaa
     7081 atattgtttc agccggagcg ggaatgccga aagcgtggcg taactgtacc ggttctgatt
     7141 tggggtttgt ttgaagtact taccaaagcc ttgtctggcg aggtacaccg gcaaggcagc
     7201 ggttgtctga gccttttgtg tttcagacgg catcagtgca gatgatgccg tctgaagacc
     7261 gtagtgaaag cggttagaaa aacggattgt gccgtttttc gtgtccgatg gaagtcatac
     7321 gcccgtgtcc ggcgacaact tgcacggttt cggggagggc gaataatttg ttgcggatat
     7381 tattgattaa gtcggcgtgg ttgccgcgcg gaaaatcggt tctgcctatg gtttcgtaaa
     7441 acagcacgtc gcccgcaatc agcaattccg cctcggcgca ataaaagacg acatgtcccg
     7501 gcgtatggcc cggaatatgc agcacttgaa aggcatagcg tccgaccgtg agcgtttcgc
     7561 cttcttcgag ccaacggttc ggcgaaaatg cgggcgagac gggaaatccg tattgcgcgg
     7621 tagtttgcgg cagcgattgg agcaggaatt catcgtccgg atgcggcccg aggacgggaa
     7681 ctttacgcgt tttcagcatt tcgaccacgc cgcctgcatg atcgagatga ccgtgcgtca
     7741 gccagattgc cgtgagcgta agtttgcggt ttgccaacgc ttgcagcagg aacggcacgt
     7801 cgccgccgac atcggtcagg acggcttcgc cgctttcgtc gtcccaaatc agggtgcagt
     7861 tttggcggaa gggggtaacg gggaagattt cgtaacgtag ggtcatcggc gtgttcctaa
     7921 acggtttttc agacggcatc gggtttgccg tttgttttat ggcggtttgc cgcccgtttt
     7981 gatattgggg gaatcagatg attaagaaga caatcggcgg catcataccg atttttacgg
     8041 cggttttcat ccctgcatcg gcaggcgcgg cggatttgat gctggcgcag gaatacaaag
     8101 ggcaggatat tgccggctgg gcaatgagcg agaaactcga cggcgtgcgc gcctattggg
     8161 acggaaagca cctgataagc cgtcagggct acgcgtttac gccccccaaa ggctttaccg
     8221 cgcagtttcc gccttatcct ttggacggcg aattgtatag cggacgtggt cagttcgagc
     8281 agatttccgc taccgtgcgt tctgtttctt cagactggcg cggcatccgc ctgcacgttt
     8341 tcgatgtacc caaggcgcag ggcaatctct accaacgttt ggcagtcgca acgcagtggc
     8401 tgaaaacgca tccgaacgcg ccgattacca tcatcccgca aaccaaagtg cgcgaccggc
     8461 agcacgcgat ggacttttta aaacaaatcg aagcgcaggg cggcgaaggc gtgatgctgc
     8521 gtcagcccga atcccgttac agcggcggca ggagcagcca attattgaag ctgaaaagcc
     8581 aatacgacga cgaatgcacg gtaacgcggc actatgaggg caaagggcga aacgccggac
     8641 ggttgggcgc gatcggctgc aaaaaccggc acggcgaatt ccgcatcggc agcggtttca
     8701 aagacaaaga ccgcgacaac ccgcccaaaa tcggcacact gattacctac cgctaccgtg
     8761 gttttacgcg gaaaggcacg ccgaaatttg ccacatttgt gcgcgtgcgt accgaccgct
     8821 gagcaggacg gttcagacgg catccgatac tttggtttat aattttcctt ttaccgaccg
     8881 attccgacat atgaccgatt tagaaaccaa acgccttgaa acacaggcga tgcttgaaaa
     8941 cgccgatctt ttgttcgacc aaggccaatg ccgtgccgca ctgcaaaaag tggcggacga
     9001 gattacgcgc gatttgggcg gcaaatatcc gctgctgctg cccgtgatgg gcggcgcggt
     9061 ggtgtttacg gggcagttgc tgccgctgtt gcgttttccc ttagattttg attatgttca
     9121 cgtttcccgt tacggcgaca agctggaggg cggcgcgttc aactggaagc gtatgcccga
     9181 tgcggaacaa atccggggca ggcacgtcgt cgtgctggac gatattttgg acgaagggca
     9241 tacgatgtcc gccattcaag ccaaactttt ggaaatgggt gcggcaagct gccgtgcggc
     9301 ggtgttcgcc aacaagctga ttgacaaacc caagcctatc cgagccgatt atgtcggact
     9361 ggatgtgccg aaccgttatg ttttcggtta cggcatggat gcggcgggct gctggcgcaa
     9421 tttgggcgag atttacgcat tggacggaaa ataaggacgc gatgccgtct gaaggctgtt
     9481 cagacggcat tgcgcccata cgccggcagg acaacgagga acaggacgca taatatgata
     9541 gggcttttaa tcatcacaca cgaaaccata ggcgaagcct accgcaagct ggcacatcat
     9601 ttttttccgg gcggactgcc tgaaaacgtc cgcatactcg gcgtgcagcc gacggaagac
     9661 caagacgaca tcatcaacaa cgccattgcc gcgcttcagg agtttcccga caacgacggc
     9721 gtgctaatta tgaccgatat tttcggcgcg accccctgca atgcagcccg ccgcctcgtg
     9781 cgcgaaaaca aatcggcgat tttgaccggg ctgaacgcgc cgatgatggt taaggccgtc
     9841 caatattcgc cggcggcgga agaccttgcc gcctttaccg aatgcgtcag ggaggcggct
     9901 gtaaaaggca ttttcgccat cacgtccgcg cccgaagatt tggtgtgccg gcgcagcggc
     9961 gatgccgtct gaagaagcgg cagggcagga aaacatttta aacggcgtct gctgccgata
    10021 tacataacac gggaaccgaa atgctcaaac aatccatcga aatcatcaac aaactcggac
    10081 tccacgcccg cgcgtccaac aagttcaccc aaaccgcgtc ccaattcaaa agcgaagtct
    10141 gggttacgaa aaacgacagc cgcgtcaacg gcaaaagcat tatggggctg atgatgctcg
    10201 ccgccgccaa gggtacggtc atcgaactgg agacggacgg cgcggacgag gcggaagcga
    10261 tgcgcgccct gaccgactta atcaacggct acttcggcga gggcgaataa tgagtatcgt
    10321 gctgcacggc gtggcggcgg gcaaaggcat tgccgtcggt tgcgcccacc tgattgcgcg
    10381 cggtacggag gaagtgccgc agtatgatgt tgcggaggcg gacaccgatg ccgaagccga
    10441 acgtttcgat gccgccgtca aagccacgcg caaagagttg gaacagctcc gcagcgcgat
    10501 tcccgaaaac gccccgaccg agttgggcgc gttcatctcg ctgcacctga tgctcttgac
    10561 cgatgttacc ttgtcgcgcg aacccgtcga tattttaagg gaacaaaaaa tcaacgccga
    10621 gtgggcattg aagcagcaga gcgacaaact cgccgcccaa ttcgacaata tggacgatgc
    10681 ctatttgcgc gaacgcaagc aggatatgct gcaagtcgtc cgccgcatcc acaacaacct
    10741 gatcgggcag ggcaacgagt tggaagttgc cgacaacctg tttgacgaaa ccgttctgat
    10801 tgcaaacgac ctttcgcccg ccgacacggt tttgtttaaa gagcagcgca ttgccgcctt
    10861 cgttaccgat gccggcggcc ccaccgggca tacggcgatt ttgggcagga gcttggacat
    10921 cccgtccgtc gtcgggctgc acaacgcgcg caaactgatt accgaaggcg aaacggtcat
    10981 tgtggacggt atcaacggcg tgttgattat cgcgccggat gagtcggtgt tgaacgaata
    11041 ccgccgccgt gcccgcgaat accgcagcca caaacgcgat ttgaacaagc tcaaaaaaac
    11101 cgccgccgcc accgccgacg gggtctgcat cgagcttgtg ggcaatatag aatccgccga
    11161 agacgtgaaa ccgctgcaca acctcggcgc agacggcatc gggctgttcc gcagcgagtt
    11221 tctttacctg aaccgcgata cgatgccgtc tgaagacgag cagtacgaag tgtacagcgc
    11281 gattgtcaaa aaaatgaaag gcaaaagcgt aacgatacgg acagtcgatt tgggtgtgga
    11341 caaaaacccg cgctggttcg ggaaaaacag cacgcccaac ggcagcctca accccgcgct
    11401 gggcatgacc ggcatccgcc tgtgccttgc cgaaccggtc atgttccgca cccagatgcg
    11461 cgccatcctc cgtgcggcgg tacacggccc cgtgcggatg atgtggccga tgattacctc
    11521 agtatccgaa gtgcgccagt gcctcatcca cctcgacacc gcgcaacgcc agcttgccga
    11581 acgcggcgat gccttcggta aagtcggcat cggctgtatg attgaaattc cgtctgccgc
    11641 gctgaccgtc ggcagtattt tgaaactggt cgatttcatc tccgtcggta ccaacgacct
    11701 gattcaatac atcttgtccg tcgatcgcgg cgacgacagc gtcagccacc tctaccagcc
    11761 cggccatccc gccgtgctga aaatgctgca acacgtcatc cgtaccgcca accgcatgga
    11821 caaagacgta tccgtatgcg gcgagatggc gggcgatacc gcgtttaccc gcgttttatt
    11881 gggtatgggg ctgcgccgtt tttccatgaa ccccaacaac atcctgcccg tcaaaaacat
    11941 cattctgcac agcaatgtcg gacagctcga aaacgagatt gccaaaatcg tccgttgcga
    12001 ggacgaggaa aaggcggaaa agctgatcaa acagatcaac agcgtgtctg tcgaggaaga
    12061 agccgacttc aaggggcgga aataaatacg gcaggtaaaa aatagaaata cttaacaatg
    12121 cccgcaatct gaaattttgc cattcttgca aaatagaaaa ccgaaacaga aacccaaaat
    12181 cgg
//
LOCUS       DAXIHS010000062        12009 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXIHS010000062 DAXIHS010000000
VERSION     DAXIHS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.062"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            139..573
                     /locus_tag="ACFOF2_001493"
     CDS             139..573
                     /locus_tag="ACFOF2_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC7107787.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(621..1964)
                     /locus_tag="ACFOF2_001494"
     CDS             complement(621..1964)
                     /locus_tag="ACFOF2_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC7107788.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1969..2622)
                     /locus_tag="ACFOF2_001495"
     CDS             complement(1969..2622)
                     /locus_tag="ACFOF2_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC7107789.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2773..3063
                     /gene="gatC"
                     /locus_tag="ACFOF2_001496"
     CDS             2773..3063
                     /gene="gatC"
                     /locus_tag="ACFOF2_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC7107790.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3126..4571
                     /gene="gatA"
                     /locus_tag="ACFOF2_001497"
     CDS             3126..4571
                     /gene="gatA"
                     /locus_tag="ACFOF2_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC7107791.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4568..5500
                     /locus_tag="ACFOF2_001498"
     CDS             4568..5500
                     /locus_tag="ACFOF2_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC7107792.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5543..6973
                     /gene="gatB"
                     /locus_tag="ACFOF2_001499"
     CDS             5543..6973
                     /gene="gatB"
                     /locus_tag="ACFOF2_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC7107793.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAESEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7065..8075
                     /locus_tag="ACFOF2_001500"
     CDS             7065..8075
                     /locus_tag="ACFOF2_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC7107794.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFOF2_001501"
     CDS             complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFOF2_001501"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC7107795.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9067..10116
                     /locus_tag="ACFOF2_001502"
     CDS             9067..10116
                     /locus_tag="ACFOF2_001502"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC7107796.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(10185..11726)
                     /locus_tag="ACFOF2_001503"
     CDS             complement(10185..11726)
                     /locus_tag="ACFOF2_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC7107797.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         3097 a         3654 c         2967 g         2291 t
ORIGIN      
        1 tgggaatctg ttttacccca atatataaag caccatatta aggcggagtg tcttccccac
       61 tttgacccga acccggaaaa gacaccgccc aagccaatcc tgatgctgcc ccgacagcca
      121 accattaagg aaatcctaat gaactttgct ttatccgtca ttatgttgac cctcgcctct
      181 ttcctgcccg tcccgcctgc cggagccgcc gtctttactt ggaaggacgg cggcggcaac
      241 agctattcgg atgtaccgaa acagcttcat cccgaccaaa gccaaatctt aaacctgcgg
      301 acgcgccaaa ccaaaccggc ggtcaaaccc gcccaagccg acgcagggaa gcgcacagac
      361 ggcgcggcac aggaaaacaa tcccgacact gccgagaaaa accggcagct tgaggaagaa
      421 aagaaaagaa ttgccgaaac cgaacggcag aacaaagaag aaaactgccg gatttcaaaa
      481 atgaacctga aggcggtggg aaattcaaat gcaaaaaaca aggatgattt gattcggaaa
      541 tacaataacg ccgtaaacaa atactgccgt taatcggctc tagcgcaaac ccgatgccgt
      601 ctgaagcggc acggggtttg tcatttctgc cagtaggttt tgacgttgac gaactcgtac
      661 agcccgaatt cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg
      721 cgcaaatcgc tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca
      781 aaccgccaag cgcgttcggt atcggcggta taaatgcagg caccgagccc gaacggggaa
      841 tcattggcaa gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      901 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      961 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
     1021 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
     1081 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttagcgca ttcggcaaga
     1141 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta
     1201 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc acgctccaaa
     1261 tcagcatcgg gcatcacgat aaaggcgttg ctaccgccga gctccaacac ggttttctta
     1321 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat gcgttgaacc ggtaaacgcc
     1381 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
     1441 agaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
     1501 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
     1561 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
     1621 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
     1681 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
     1741 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1801 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1861 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg
     1921 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1981 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     2041 catacccata atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     2101 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat
     2161 caacgcgatg tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc
     2221 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     2281 ggcggggcgc gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     2341 gttgccgttc atcatagaaa tggcaatcag caacaccaaa attccgccgc cgacctgaaa
     2401 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     2461 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     2521 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattga tcagcaccaa
     2581 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     2641 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     2701 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     2761 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2821 ctgcacctga ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact
     2881 atggtcgaac agatgcagaa catcaacaca gacggcatcg aaccgatggc gcacccgcac
     2941 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     3001 caggcggtcg caccggaagt acgcgaccgc ctgtacatcg taccgcaagt tatcgaagaa
     3061 taatccgaat acgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     3121 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     3181 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     3241 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     3301 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     3361 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     3421 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     3481 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     3541 aaaacccatg gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg
     3601 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac
     3661 ccgcatcgca ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg
     3721 gtatggtcgc ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag
     3781 actgcgcgat tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg
     3841 agcgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg
     3901 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa
     3961 acaccattga tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa
     4021 ccaagctgtc catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt
     4081 cacgttacga cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa
     4141 tgtacggcaa aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg
     4201 gcacttatgt actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     4261 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     4321 cgaccgcacc cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact
     4381 tgagcgatat ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     4441 caggcttcag cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg
     4501 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     4561 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     4621 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     4681 agaaatttac gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct
     4741 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4801 caaacgccgt ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac
     4861 agacatcctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4921 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4981 atctttgcgc gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac
     5041 acacctgctg acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg
     5101 ccgcataacc aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     5161 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     5221 caaactgcgc tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga
     5281 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     5341 agaccttacc gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct
     5401 ggacaacatg gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga
     5461 cggcatccaa acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga
     5521 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     5581 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     5641 caacgcgcac gccggcgtag tggaatgtgc tttgccgggc gtactgccgg taatgaaccg
     5701 agaagtcgtt gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa
     5761 aaacgtgttc gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag
     5821 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5881 gaaaaccatc aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga
     5941 aggcttgaac ggcgcgaccg gtatcgattt gaaccgcgcc ggcacgcctt tattggaagt
     6001 cgtatccgaa cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca
     6061 cagcttggta acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt
     6121 cgatgccaac gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat
     6181 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca
     6241 aatcgagatt ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga
     6301 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc
     6361 cgaccccgat ttgctgcccg tcatcatttc agatgcccaa atgcaaaaag ccaaagcaga
     6421 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga
     6481 atacgacgcg cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc
     6541 caaagagagc ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc
     6601 actgaacaaa gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc
     6661 gctggtaggc aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga
     6721 agccatgtgg gcagaatccg aagccaccat tgccgaaatc attgaaaaac acggtttgca
     6781 gcaaatgacc gataccggcg cgattgaagc catggtggac gaagtgctgg caaacaacgc
     6841 caaagccgtg gaacagttca aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6901 agtgatgaag gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6961 caaacttgcc tgacaagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt
     7021 ttcacagtct gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta
     7081 ccctgcccga ccaaaccacc tttgccgcca acgacggcga aaccgttttg gccgctgccg
     7141 cccgacaaaa cctcaacctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag
     7201 ccgaactggt cagcggcgat attcaaatgg acggacactc ggaacaggct ttatccgaag
     7261 cagaaaaagc gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca
     7321 tcaacatccc cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg
     7381 aaagtattat tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc
     7441 cgtttgcctt ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca
     7501 gctactccat cgccaatccg cccgaccaag aaggcatttt ggaactacac atccgcaggc
     7561 gcaaaaacgg tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag
     7621 gcatcgtccg cgttaaaggt ccgctcggtt cgtttaccct gcaggaagat agcggcaaac
     7681 ccgtcatcct gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc
     7741 ttatccgcca aaacagcagc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg
     7801 atttgtatgc cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca
     7861 cccccgtatt gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca
     7921 tcgcggcaca agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg
     7981 ccatgaccga acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt
     8041 ttttctccga cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg
     8101 ctttccgttc ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct
     8161 gaagcccgat tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct
     8221 cgcgtatcca gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc
     8281 tgccctgcca aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac
     8341 gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct
     8401 tgttcggaat cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc
     8461 ggctctcaaa atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc
     8521 gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca
     8581 gcgaacgtcc cttacggctg tgataattcg taaagaaaac aaaaccttca gaattaactt
     8641 ccttcagcag caccatacgg ctgttgggtc tgccgcgtcc gtcgaccgcc gccacattaa
     8701 ccgccgtcgg ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct
     8761 cgatcggatt gtcggcgcaa tcgccttccg acaattcccg tttactgtaa tcttcccgaa
     8821 tattgtgcaa atccatttac cgctcctctt gtcatttgaa agattctacc cccgcacgca
     8881 aaccgatttc aaccgttgca caaactttgc ctcgcttctc caaacctcag gacacaacga
     8941 tttcatccgc aaaaccgccg cataaggtac aatatcgaac cgtccgaccg agggcggcat
     9001 tttatcaacc cgtcctgccg cacacgccgc agaagaaccg ccttatcagg cgagttagga
     9061 aaaatgatgt ccaaacagcc caccagcaaa cgccaatggc gcgacggcgt agccccgtct
     9121 gccaagaaaa ccgccaaacc tttcaaaagc aaagcccgtc ccaaagacga aacggacaaa
     9181 accgcatccc aaccttacgg acaaaaagct tcagacggca tcaaacctca aaacctcccc
     9241 aaacagcgcg ccgccaaagc caaaaaactc gtcgtccgca atcccaacca aaaaattatg
     9301 gaacacgcgc gcgatttaaa agaacgccgc agcgacctat cgcgcatgga acccgaacgc
     9361 ctgcaaaaag tgcttgccgc gtccggcgtc ggctcgcgcc gcgaaatgga agaatggatc
     9421 aacaacggct gggtaacggt caacggcaaa accgcgcaac tgggcgacaa agtcaccccc
     9481 gacgaccacg ttaccgtcaa aggcagcatc atcaaactca aatgggccga ccgcctgccg
     9541 cgcatcatcc tatattacaa acaagaaggc gaaatcgttt cccgcgacga cccgcaaggc
     9601 cgcgtcagca tattcgaccg cctgccgcag gccgccagca gccgctgggt cgccatcgga
     9661 cgcttggaca tcaacaccag cggacttctg attctcacca cctccggcga actcgtccaa
     9721 cgtttcgccc accccagctt cgaagtcgaa cgcgaatacg ccgtgcgcgt actgggcggg
     9781 ctgaccggcg aacaaatgcg cgtcctcacc gaagaaggcg tgatgctcga agacggcttg
     9841 gcaaaagtcg aacgcatccg cgaacaaggc ggcgaaggcg cgaacaaatg gtacaacgtc
     9901 gtgattaaag aaggccgcaa ccgcgaagtg cgccgcattt ttgaaagcca aggactcacc
     9961 gtcagccgcc tcgtgcgcgt cggcttcggc cccatcggac tgcccaaccg cctcaaacgc
    10021 ggacagttct acgaactcaa ccccgccgaa gtcgccaaca tcctcaaatg ggcggaaatg
    10081 ctgctgccgg gcgaacgccg ccgcaaaaaa gcctaaaccc gccaaaacac aaaaatgccg
    10141 tctgaaacat ctactgtttc agacggcatt ttattcgggc gttttcagga gaaaaggtcg
    10201 agtgctttga caaagaccat caccacgccg taggcgagcg gtatgacggc caatgcccaa
    10261 cgccaccaga ccgatatgcc gccgccggaa actttttcgc ccacaaggta agcgtcggat
    10321 atggcggttt cgtcatcggg gttgccgctg tgtgcggcgg ttttgatgtc tttttcgtgg
    10381 tgtttttcgt gtacggattt gacggcgagg ttgcacaaca aaccgataat cagcaggcac
    10441 gccatgatgt acatggttac gctgtatgcc tgtgccgccg gtatgccgct gtcgatttgg
    10501 ctttggcgta tgtaattgac cagtaccggg ccgatgacgg cggcggttga ccaggccagc
    10561 aggatgcgcc cgtgaatcgc gccgacctga taggtgccga acaggtcttt caggtaggcg
    10621 ggaatggcgg caaatccgcc gccgtacatg gaaataatca cgcaaaagcc gatgatgaac
    10681 agggctttgc tgccgccctc gccgatggag ggaacggcga aatacagcag cgaaccgagt
    10741 acgaagaaaa tggtgtaggt gtttttgcgt ccgattttgt cggaaacgct cgaccataaa
    10801 aagcgtccgc ccatgttaaa caggctcagg aggctgacga agcctgccgc cgcacctgcg
    10861 ccgactgacg cctgcctgcc tacggaggtt tcggaaaaga gttcctgaat catcacggat
    10921 gcctgaccca atacgccaat gccggcggtt acgttcaggc acaataccca gaacaacagc
    10981 caaaattgcg gcgttttcat ggcttgtgac acgttgacat gattgctgct gaccagcttg
    11041 ttttgcgttt tcggcgcggt atagccttca ggtttccagc cgtcggcagg tacgcggatg
    11101 gtaaacgcgc cgaacatcat cagtgcgagg taaagcaggc ccaatacggc gaaggtttca
    11161 acaaccccga ctgaagcggc gtttgaaaag gcgttcatca gtgatacgga aagcggagag
    11221 gccagcattg cgccgccgcc gaaacccata atcgccaaac cggtcgccat acccggcttg
    11281 tcgggaaacc atttcatcag tgtggaaacc ggcccgatgt agcctaagcc taagcctacg
    11341 ccaccgatga cgccgttgcc caaataaagc aggaagaggt tgtgcgtact cacgccgaat
    11401 gcggatacga agaagcccag gctgaaacag caggcggcgg caaatatggc tttgcgcggc
    11461 cctacccgtt ccatccatgt gccgaacagg gcggccgacg cgcccagcat cgcgagtgcg
    11521 atactgaaaa tccaacctac ggtcgtcagc ttccaatctc cggccgccga ttcggttatg
    11581 ccgataagtt tggtcagcgg cgcgttgaat acggaatagg cgtaaatctg cccgatggca
    11641 aggtgtaccg ccaatgctgc gggcggtacg agccaacggt tgaaacccgg cttggcaatg
    11701 cttgcctcac ggtctaaaaa cttcataaca tcctctttct gtcagttgaa aaataaaatt
    11761 tcatttgctc aatggaaact tattgaaaac tataaaaaaa tatcggatcg gttttttatc
    11821 cgccccaaga tgccccgtct gaaacatttc gggtgtacgg aaaggtttct gttttttccg
    11881 acaaattcct gcggcttttc gcttccggat tcccgctttt gcgggaatga cgaattaaag
    11941 attatcttaa ggtcaaggga ctggattccc gcttttgcgg gaatgacggc ggcgggttgc
    12001 tgtttttta
//
LOCUS       DAXIHS010000063        11879 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXIHS010000063 DAXIHS010000000
VERSION     DAXIHS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.063"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            321..4283
                     /gene="purL"
                     /locus_tag="ACFOF2_001504"
     CDS             321..4283
                     /gene="purL"
                     /locus_tag="ACFOF2_001504"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC7107798.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDDEKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPCLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            4378..5136
                     /gene="gloB"
                     /locus_tag="ACFOF2_001505"
     CDS             4378..5136
                     /gene="gloB"
                     /locus_tag="ACFOF2_001505"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC7107799.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            5443..9916
                     /locus_tag="ACFOF2_001506"
                     /pseudo
     CDS             5443..9916
                     /locus_tag="ACFOF2_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            10148..11602
                     /gene="mgtE"
                     /locus_tag="ACFOF2_001507"
     CDS             10148..11602
                     /gene="mgtE"
                     /locus_tag="ACFOF2_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC7107800.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(11688..11801)
                     /locus_tag="ACFOF2_001508"
                     /pseudo
     CDS             complement(11688..11801)
                     /locus_tag="ACFOF2_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated molecular chaperone Hsp33"
BASE COUNT         3057 a         3704 c         2896 g         2222 t
ORIGIN      
        1 ttcagcaata tttaaaggtt tctgtaaatc caaacttcca tattcctaac cgtacggaaa
       61 cgacggcatt tgatctaaaa acatacggaa gcagttgccc gcccctctac ttcctgcaat
      121 tatccgcata cccggctttg ggtaaaaatc agaaaaacag ataaaaccct tattccttaa
      181 tatcttcaga cggcacctgt attttcccca actgtatgcc gtctgaagat tgtcaacatt
      241 ttaagccaaa atacccaagc aatacagccc cgttgcgcgt ataatgacag attttccaac
      301 cgcatttgag agccgaatcc atgtctgtcg ttttgccctt gcgcggcgtt accgcccttt
      361 ccgatttccg tgtcgaaaaa ctcttgcaaa aagccgccgc actcggactg cccgaagtca
      421 aattaagcag cgaattttgg tatttcgccg gcagcgagaa agcattggat gccgcgactg
      481 tcgaaaaact gcaagccttg ttggcggcgc aaagcgttga acaaacgcca aaagcgcgcg
      541 agggcttgca tttgtttttg gtaacgcccc gtttgggtac gatttcgccg tgggcttcca
      601 aggcgaccaa tatcgcggaa aactgcggtt tggcaggcat cgaacgcatc gagcgcggca
      661 tggcggtgtg gctggaaggt cgtctgaacg acgatgagaa acagcaatgg gcggcgttgc
      721 tgcacgaccg catgaccgaa tccgtgctgc ccgattttca gacggcatcc aaattattcc
      781 accatctcga atccgaaacc ttttccaccg ttgatgtttt aggcggcggt aaagaggctt
      841 tggtcaaagc caataccgaa atgggtttgg cactctccgc cgacgaaatc gattatctgg
      901 ttgaaaacta tcaggctttg cagcgcaatc cgtccgatgt tgaattgatg atgttcgcgc
      961 aggcaaacag cgaacactgc cgccacaaaa tcttcaacgc cgatttcatc ctcaacggcg
     1021 aaaaacaacc gaaatccctg ttcggcatga tacgcgacac gcacaacgcg catcccgaag
     1081 gtacggtcgt cgcttataaa gacaattcgt ccgtaatcga aggcgcgaaa atcgagcgtt
     1141 tctatccgga tgcggcggaa aaccaaggct accgtttcca cgaggaagac acgcatatca
     1201 tcatgaaagt ggaaacgcac aaccacccga ccgccatcgc gccgtttgcg ggcgcggcaa
     1261 cgggcgcggg cggcgaaatc cgcgacgaag gtgcgacggg caaaggttcg cgtccgaaag
     1321 caggcttgac cggctttacc gtgtccaacc tcaacatccc ctgcctcaaa cagccgtggg
     1381 aacaagatta cggcaagccg gaacatattt cctcgccgct ggacattatg attgaaggcc
     1441 ccatcggcgg cgcggcgttc aacaacgaat tcggccgccc caacctcttg ggctacttcc
     1501 gcacctttga agaaaaattt gacggtcagg ttcgcggcta ccacaaaccg attatgattg
     1561 ccggcggtct gggcagcatt caggcgcagc agacgcataa agacgaaatc cccgaaggcg
     1621 cattgctgat tcaactgggc ggcccgggca tgcttatcgg cttgggcggc ggcgcggctt
     1681 cttcgatgga taccggcacg aacgacgcgt ctttagactt caattccgta caacgcggca
     1741 accccgaaat cgaacgccgc gcgcaggaag tcatcgaccg ctgctggcag ctcggcggca
     1801 aaaacccgat tatctccatc cacgacgtgg gcgcgggcgg tttgtccaac gccttccccg
     1861 aactcgtcaa cgatgccgga cgcggcgcgg tattcaagct gcgcgaagtg ccgttggaag
     1921 aacacggttt aacgcctttg caaatctggt gtaacgaatc gcaagagcgt tatgtgttgt
     1981 cgattttgga aaaagatttg gacaccttcc gcgccatctg cgaacgcgaa cgctgcccgt
     2041 ttgccgtggt cggcacggcg accgacgacg gccatttgaa agtgcgcgac gatttgttcg
     2101 ccaacaaccc cgtcgatttg ccgctgaacg tcttgctcgg caaaccgccc aaaaccacgc
     2161 gtaccgacaa aacggttacg ccgtccaaaa aaccgtttca cgccgacgat atcgacatca
     2221 ccgaagccgc ctaccgcgtt ttgcgcctgc ctgccgtagc cgccaaaaac ttcctgatta
     2281 ccatcggcga ccgcagcgtc ggcggcatga cccaccgcga ccaaatggtc ggcaaatacc
     2341 aaacccccgt agccgactgc gccgttacca tgatgggctt caatacctat cgcggcgaag
     2401 caatgtctat gggcgaaaaa ccgaccgttg ccctgtttga cgcgcccgcc tcgggcagaa
     2461 tgtgcgtcgg cgaagccatc accaacatcg cggcggtcaa catcggcgac atcggcaata
     2521 tcaaactctc cgccaactgg atggcggcgt gcggcaacga aggcgaagac gaaaaactct
     2581 accgcaccgt cgaagcggtt tccaaagcct gtcaggcatt ggatttgagc attcccgtgg
     2641 gcaaagacag cctgtcgatg aaaaccgtat ggcaggatgg cgaagagaaa aaatccgtcg
     2701 tttcgccgtt aagcctgatt atctccgcgt tcgcgccggt tcaagacgta cgcaaaaccg
     2761 ttacgcccga attgaaaaac gtcgaggaca gcgtattgct gtttgtcgat ttgggcttcg
     2821 gcaaagcgcg tatgggcggc tctgcattcg gtcaggtata caacaacatg agcggcgacg
     2881 cgcccgattt ggacgacaca ggccgtctga aagcgtttta caacgtgatt caacagcttg
     2941 tcgccgaaga caaactcttg gcataccacg accgcagcga cggcggcttg tttgccgttt
     3001 tggtagaaat ggcgtttgca gggcggtgcg gcttggatat agatttaaat ttattgcttg
     3061 cacaaacatt tattaccaac cataccgctc tgtctcaatc attgcggact gaagaggtaa
     3121 aagcgttggc tgaatggcaa gaaaccattg cccgcacatt atttaatgaa gagttgggtg
     3181 ctgttatcca agttagaaaa caagatgttg ccgatattat caatttattc tatcaacaac
     3241 agctgcatca taatgtcttt gaaatcggta cgttaactga tgagaacacg ttaatcatcc
     3301 gcgacgggca aacgcacctt atttctgaca acctaatcaa actgcaacaa acctggcaag
     3361 aaaccagcca tcaaatccaa cgcctgcgcg acaaccctgc ctgcgccgac agcgagtttg
     3421 ccctgattgg cgacaacgac cgcagcgcat tgtttgccaa cctgaaattc gacgtgaacg
     3481 aagacatcgc cgcgccgttt atcaacagcg gcgcgaaacc caaaatcgcc atcctgcgcg
     3541 aacagggcgt gaacgggcaa atcgaaatgg ccgccgcgtt cacccgtgcc ggattcgatg
     3601 cctacgacgt gcatatgtcc gacctgatgg caggccgctt ccgccttgcc gacttcaaaa
     3661 tgctggcggc gtgcggcggc ttcagctacg gcgacgtact cggcgcgggc gaaggctggg
     3721 cgaaatccat cctgttccac cctgctctgc gcgaccagtt tgccgccttc ttcgccgacc
     3781 cggacacgct gacattgggc gtgtgcaacg gctgccaaat ggtcagcaac ctcgccgaga
     3841 ttatcccggg tgcagaggca tggccgaagt tcaaacgtaa cttgagcgaa cagttcgaag
     3901 cgcgcctgag tatggttcac gtccccaaat ccgcgtcgct gattctgaac gaaatgcaag
     3961 gctccagcct gcccgtcgtc gtcagccacg gcgaaggccg cgccgacttc gcgcttcacg
     4021 gcggcaacat ttctgctgat ttgggcatcg cgctgcaata tgtggacgga caaaaccagg
     4081 ttacccaaac ctatccgctc aaccctaacg gctcgccgca aggcatcgcc ggtgtgacca
     4141 acgccgacgg ccgcgtgacc attatgatgc cccaccccga acgcgtgtac cgtgccgcgc
     4201 aaatgagctg gaaaccggaa ggctggacgg aactgtccgg ctggtaccgc ctctttgccg
     4261 gcgcacgtaa agccttgggc taaccgccct actcaaacca atgccgcctg aagaatattt
     4321 cagacggcgt tccggcatac catcctttaa acggtatccg tccaccgagg aacactcatg
     4381 aaaatcaccc ccgtcaaagc cttgaccgac aactacatct ggatgataca gcacggcaac
     4441 catgccgtct gcgtcgaccc ttccgaaccc tcgcccgtct tggaattcct cgtccgcaac
     4501 cgcctcatgc ttgcccaaac atgggtaact cacccccatc ccgaccacga gggcggtgcg
     4561 gcggcactct ggcgcggcta catggaatcg cccgtttacg gcgaatccga catcgaagca
     4621 gcaacccaca ccgtaaccgc cggcacccaa ttcaccttcg gcgacggaca ggttaccgtt
     4681 tgggcaacac ccggccacac agaccgccac accagctacc ttctcgaaac ttcagacggc
     4741 atacacgtct tttgcggcga cacccttttt tccgccggct gcggacgcgt gtttaccggc
     4801 acaatcgaac agctttacga cagcttccaa cgcttcaacc gcctgcctga aaacaccctg
     4861 ttctatccgg cgcacgaata caccgccgcc aacctggatt ttgccgccca tatcgagccg
     4921 gacaacgccg acattcagac ggcactgaaa gcggcggcgc atacgcctac cctgcccgtt
     4981 accctcgcgc acgaacgccg tgtcaatccg tttttgcgcg tcgacctgcc gcacgtcaga
     5041 gaccgcgccg aggcattgag cgggaaaacg ttaaacagca gcctcgatac ctttgtcgcg
     5101 ctgcgtgaac ttaaaaacca ataccggacg aaataaaaca acgggaaaac gcagccattc
     5161 ctaggatttt tattaaaatc ttaaataaaa tcatacaatc atcgccaata gacgaaagga
     5221 caccgttgcc ttataatcaa acaaaaacaa aatatataat atagtggatt gaatttaaat
     5281 caggacaagg cgacgaagcc gcagacaata cagatagtac ggcaaggcga ggcaacgctg
     5341 tactggtttt tgttaatcca ctatataaat ccagcacaaa acgggatcgg tgattcttgt
     5401 ccgcaagaat cgttgatttt ctctattaca cggataatca tcatgcgctt cacacacacc
     5461 accccatttt gttccgtatt gtccaccctc ggtctttttg ccgtttcccc tgcttactca
     5521 tccattgtcc gcaacgatgt cgattaccaa tattttcgcg actttgccga aaataaaggc
     5581 gcgttcgccg taggtgcaag caatatttcc atccaagaca agcaaggcaa aatattaggc
     5641 agggttctca acggcatccc catgcccgac ttccgcgtca gcaaccgcca aaccgccatc
     5701 gccaccctgg ttcaccccca atacgtcaac agtgtcaaac acaacgtcgg ctacggttcc
     5761 atacaattcg gcaacgacac ccaaaatcca gaagaacaag cctataccta ccgcctcgta
     5821 tcacgcaacc cgcacccgga ctacgactac caccttcccc gcctcaacaa actggttacc
     5881 gaaatctcac ctgccgcact cagcagcgta cccttgcttg gaaacggcca gccaaaggcc
     5941 aatgcctacc tcgataccga ccgcttccca tactttgtac gactcggctc aggcacgcaa
     6001 caagtccgca aagcagacgg cacgcgtaca cgaaccgccc cggcatacca atacctgacc
     6061 ggcggcacgc cgctgaaagt attggggttc caaaaccacg gcttactcgt cggcggcagc
     6121 ctgaccgacc aaccccttaa cacctacgca atcgccggag acagcggttc ccccctgttt
     6181 gccttcgaca agcatgaaaa ccgctgggtg cttgcgggcg tactcagcac ctacgccggc
     6241 ttcgataatt tcttcaacaa atacatcgtc acgcaacccg aattcatccg ttccaccatc
     6301 cgccaatacg aaacccggct ggatgtcggg ctgaccacca acgaactcat atggcgcgac
     6361 aacggtaatg gcgacagcac cctgcaaggg ctcaacgaac gcatcaccct gcccattgca
     6421 aacccttcgc ttgccccaca aaacgacagc aggcacatgc cgtctgaaga tgccggcaaa
     6481 acgctcatcc tatccagcag gttcgacaac aaaacactga tgctggcaga caatatcaac
     6541 caaggcgcag gcgcattgca gttcgacagc aacttcaccg tcgtcggtaa aaaccacaca
     6601 tggcaaggtg caggcgttat cgtagccgac ggcaaacgcg tcttctggca agtcagcaac
     6661 cccaaaggcg accggctctc caaactgggc gcaggcacgc ttatcgccaa cggacaaggc
     6721 atcaaccagg gcgacatcag catcggggaa ggcactgtcg tactcgccca aaaagctgct
     6781 tcagacggca gcaaacaagc attcaaccaa gtcggcatca ccagcggcag gggcacggcc
     6841 gtcctcgccg acagccagca aatcaaaccc gaaaacctct atttcggctt caggggcgga
     6901 cggctcgacc tcaacggcaa caaccttgcc tttacccata tccgccatgc ggacggcggc
     6961 gcgcagatcg tcaaccacaa ccctgaccaa gccgcgacac tgacgctgac cggcaacccc
     7021 gtcctcagtc ccgagcatgt cgagtgggtg caatggggca accgtccgca aggcaacgcg
     7081 gcggtttacg aatacatcaa cccgcaccgc aaccgtcgga ccgactactt catactcaaa
     7141 cccggcggca acccgcgcga atttttcccg ttaaatatga aaaactcaac aagctggcaa
     7201 tttatcggca acaacaggca acaggccgcc aaacaagtcg cccaagccga aaatgcccgc
     7261 cccgacctaa ttaccttcgg cggatacttg ggtgagaacg cgcaaacggg caaagccgca
     7321 ccgagttaca gcaaaaccaa tatcgaatgg gtgaatggtc caaacacccc acacaagccc
     7381 tcgccctata cgaatacgcc aacccgcaca ggggcaggcg taccgacgat taccgcctca
     7441 aagaaagcgg caaccccggc gcctacttcc catggaatgc ctacagcacc caagcctggg
     7501 aatttctcgg acatgaccgg cgaagcagcc atagaaaaaa cccgccatat cgcaaatgcc
     7561 gccgtatacg gcaggcccga ataccgttac aacggcgcac tcaacctgca ctatcgtccc
     7621 aaacgcaccg acagcacgct gttgctcaac ggcggcatga accttaacgg ggaagtcttg
     7681 attgagggcg gcaatatgat tgtgtcaggc aggcccgtac cccatgccta cgaccaccag
     7741 gccaaacgcg aacccgttct tgaaaacgaa tggaccgacg gcagcttcaa ggctgcacgg
     7801 ttcaccctgc gaaaccatgc ccgactgacg gcagggcgca ataccgcgca tctggacggc
     7861 gacataaccg catacgatct gtccggcatc gacctcggct ttacccaagg caaaacaccg
     7921 gaatgctacc gctcctacca tagcggcagc acccactgca cacccaacgc cgttttaaaa
     7981 gccgaaaact atcgtgcact acctgcaacg caagtacgcg gcgacattac ccttaacgac
     8041 cgttcagagc tccgcctggg caaagcacac ctgtacggca gcatccgtgc cggcaaagac
     8101 accgcagtcc gcatggaagc agacagcaac tggacacttt cccagtccag ccacaccggc
     8161 gcactgacgc ttgacggcgc acaaattacc ctgaaccccg atttcgccaa taatacacac
     8221 aacaaccgct tcaacacact gaccgtcaac ggcacacttg acgggttcgg cacattccga
     8281 ttcctgaccg gcatcgtccg aaaacaaaat gccccccctc aaactggaag gggacagccg
     8341 cggcgcattc caaatccacg tcaaaaacac cggacaagaa cctcaaacaa ccgaatcgct
     8401 tgcacttgtg agcctcaatc cgaaacacag ccaccaagcc cgattcaccc tccaaaacgg
     8461 ctatgccgat ttgggtgcct accgctacat cctccgcaaa aacaacaacg gatacagcct
     8521 gtacaacccg ctcaaagagg ccgaacttca aattgaagcc acgcgtgcgg aacatgagcg
     8581 caaccaacag gcatacaacc aattacaggc aaccgacatc agcagacagg ttcaacatga
     8641 ctctgacgcg accaggcagg cactacaggc ctggcagaac agtcaaaccg aacttgcccg
     8701 catcgacagc caagtccaat atctgtccgc ccaattgaaa cagacagacc cgctgaccgg
     8761 cattctgacg cgtgcccaaa acctgtgtgc cgcacaagga tacagtgtcg atatctgccg
     8821 tcaggttgcc aaagccgccg acacgaacga cctgacactc ttcgaaaccg aactggatac
     8881 gtatatagaa cgtgtagaaa tggccgaatc cgaacttgac aaagcacggc aaggcggcga
     8941 tgcgcaagcc gtcgaaacag cccggcacgc ctacctgaac gcactcaacc gtctgtcccg
     9001 acaaatccac agtttgaaaa ccggcgttgc cggcatccgt atgccgaacc tggccgaact
     9061 gatcagccgg tcggccaaca ccgccgtttc cgaacaggcc gcctacaata ccggccggca
     9121 acaggcggga cgccgcatcg accgccacct taccgatccg cagcagcaaa acatctggct
     9181 ggaaaccggt acgcaacaaa ccgactacca tagcggcaca caccgtccct accaacaaac
     9241 taccaactat gcacatatcg gcatccaaac cggcatcacc gaccgtctca gtgtcggtac
     9301 gattttaacc gatgagcgca caaacaaccg ttttgatgaa ggcgtatccg cccgaaaccg
     9361 cagcaacggc gcacatctgt tcgtcaaagg ggaaaacggc gcactctttg ccgcggcaga
     9421 tttaggctac agcaacagcc gtacccgatt taccgattat gacggggctg ccgtccgccg
     9481 ccacgcatgg gatgcaggca tcaacaccgg catcaaaatc gataccggca tcaacctcag
     9541 accctatgcc ggcatccgta taaaccgcag caacggcaac cggtacgtac tcgacggcgc
     9601 agagataaac agcccggcgc aaatccaaac cacatggcat gccggcatcc gtctcgataa
     9661 aaccgtcgaa ctgggtcaag ccaagctgac ccccgctttc agcagcgatt actaccatac
     9721 ccgccaaaac agcggttccg ccctcagcgt caacgaccgt accttactgc agcaagccgc
     9781 ccacggcaca ctgcataccc tgcaaatcga cgccggatac aaaggctgga acgccaaact
     9841 tcatgccgct tacggcaaag acagcaacac cgcccgccac aaacaggcag gaatcaaaat
     9901 aggctacaac tggtaacaag ccggtaaaaa tgccgtctgg aacccgcgtt tcagacggca
     9961 tttgcgttaa aaatagtaaa ccgtttcaaa agggagtaga atagtgccgt ttccaaccct
    10021 gcgcctgtac cgtcaggctt ttattatgga ccttcccagt tcgtttttac tgaacgcccc
    10081 ttccgattcc aaacgacaat aaccatcccg ccggaatgcc tccccgcaca cggcgggcgg
    10141 agcatttatg agcatcgaac acactcctcc gacacacgac ggcgaaaccg gtcaaaacca
    10201 tgccgaacgc ccttccgccg atttcgaccg tgtccactcc ctctgcgaaa tcctcgaacc
    10261 tgcttttgaa caaatcgaaa acggcacacc gctcgaagac gcgccgctgc gcgacaagct
    10321 gaccgagctg accgtcctct tgggcgagct gcaccctgcc gacgtggcgg cggtgttgga
    10381 atcgctgccg ccgcgcgaac gcaacgtcgt ttggcttctg gtcaaacccg aagacgacgg
    10441 cgaagtattg ctggaagtat ccgacgcggt gcgcgaaaca ctgatcgagt cgatggataa
    10501 ggacgaattg ttggcggcag ttgacgattt ggacgcggac gaattggcgg aactggcaga
    10561 cgatttgccg caccaagtgg tttacgaagc cttacagacg cgtgatgagg aagaacgcgc
    10621 ccaagtcaag gcggcaatgt cctacgaaga caaccaagtc ggtgcgatta tggacttcga
    10681 gttggtcagc atccgcgccg atgtcgcctg cgaagtggtg ctgcgttacc tgcgccgctt
    10741 cgacagcctg cccgaccaca cggataaaat cttcgtggtt gacgaaaacg atgtgttgca
    10801 gggcgtgctg cccatccgca aacttttggt cgccgatccc gaagacttgg tggaaaacgt
    10861 gatggcgaaa gatgtcgtgc gtttccgcgc cgaagacgac gtggaagaag cagcgcaggc
    10921 gtttgaacgc tatgacttgg ttaccgcgcc cgtcgtcgat gaaaacaaaa agctcatcgg
    10981 cagggtgacc gttgacgaaa tggtggacgt gatccgcgaa gaatccgaag cagatatgtt
    11041 gaacatggcg ggtttgcaag aagaggaaga cttattcgcc cccgtcttgg attccgtcaa
    11101 aaaccgctgg atgtggctcg ccgtcaacct ctgcaccgcc ttccttgcca gccgcgtcat
    11161 cggcgcgttt gaaggcagca tcgaaaaaat cgtcgcactc gccgcgctga tgcccatcgt
    11221 cgccggtatc ggcggcaact cgggcaacca gacgattacc atgattgtcc gcgccatggc
    11281 gatggggcaa ctgacaggca tgcaggcagg caggctgttg aaaaaagaag tcggcgttgc
    11341 cctagtcaac ggcattatct ggggaaccgt catgggcgcg gtttcatggc tgctctacgg
    11401 cagcctcggc atcgggctgg ttatgattgc cgcgatgacg ctcaacctcc tgctggcggc
    11461 aaccgtcggc gtattaattc ccgtggtaat ggaaaagttc ggacgcgatc ccgcactggg
    11521 cagctcggtg ctgattaccg ccgttaccga ctccggcggc ttcctgattt tcttgggtct
    11581 cgccaccctg ttcctgcttt aaatgccgtc tgaacccgcg caaaaatgcc gtctgaagcg
    11641 gaagctgctt cagacggcat ttgactattt atccttgttg cacaagatta ttggacggta
    11701 tgccggggca gccctttggc aacgccgacc acatcctccc cgaacagcgc gttgacatcg
    11761 gtttcgtcaa acacatattt gctgagacct ttgcaaaatt cctttccctc acgacaaccg
    11821 aaatcccaac acaggttttc ggctgttttc gccccaaata ccgcctaatt ttacccaaa
//
LOCUS       DAXIHS010000064        11769 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXIHS010000064 DAXIHS010000000
VERSION     DAXIHS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.064"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..1377
                     /locus_tag="ACFOF2_001509"
     CDS             <1..1377
                     /locus_tag="ACFOF2_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC7107801.1"
                     /translation="KHTKDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSN
                     AENDSDPLADSDPFYEFFKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHV
                     VTGMGSIKVLLNDKREYTAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWV
                     AAIGAPFGFDNSVTAGIVSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVG
                     INSQIYSRSGGFMGISFAIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFG
                     LDKAGGALIAKILPGSPAERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSL
                     GVWRKGEEITIKVKLGNAAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSG
                     GHLVVVRVSDAAERAGLRRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNT
                     LFIALNLQ"
     gene            1579..3099
                     /locus_tag="ACFOF2_001510"
     CDS             1579..3099
                     /locus_tag="ACFOF2_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC7107802.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            3156..4241
                     /gene="nagZ"
                     /locus_tag="ACFOF2_001511"
     CDS             3156..4241
                     /gene="nagZ"
                     /locus_tag="ACFOF2_001511"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC7107803.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAALASPQDTAGGVKVGEAF"
     gene            4322..4398
                     /locus_tag="ACFOF2_001512"
     tRNA            4322..4398
                     /locus_tag="ACFOF2_001512"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4356..4358,aa:Pro,seq:cgg)
     gene            complement(4733..5371)
                     /locus_tag="ACFOF2_001513"
     CDS             complement(4733..5371)
                     /locus_tag="ACFOF2_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC7107804.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5368..5739)
                     /locus_tag="ACFOF2_001514"
     CDS             complement(5368..5739)
                     /locus_tag="ACFOF2_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC7107805.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGVTVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(5844..5969)
                     /locus_tag="ACFOF2_001515"
     CDS             complement(5844..5969)
                     /locus_tag="ACFOF2_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107806.1"
                     /translation="MKSMDIRFARIAALTKQLAAIQRQIEKRAYKAKAATMKKVA"
     gene            complement(5989..6345)
                     /locus_tag="ACFOF2_001516"
     CDS             complement(5989..6345)
                     /locus_tag="ACFOF2_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107807.1"
                     /translation="MNTTSSMIQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            complement(6454..6876)
                     /gene="queD"
                     /locus_tag="ACFOF2_001517"
     CDS             complement(6454..6876)
                     /gene="queD"
                     /locus_tag="ACFOF2_001517"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC7107808.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(6883..7401)
                     /locus_tag="ACFOF2_001518"
     CDS             complement(6883..7401)
                     /locus_tag="ACFOF2_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC7107809.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(7429..8088)
                     /gene="queC"
                     /locus_tag="ACFOF2_001519"
     CDS             complement(7429..8088)
                     /gene="queC"
                     /locus_tag="ACFOF2_001519"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC7107810.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            complement(8292..8478)
                     /locus_tag="ACFOF2_001520"
                     /pseudo
     CDS             complement(8292..8478)
                     /locus_tag="ACFOF2_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            8633..9859
                     /locus_tag="ACFOF2_001521"
     CDS             8633..9859
                     /locus_tag="ACFOF2_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC7107811.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(9880..10161)
                     /locus_tag="ACFOF2_001522"
     CDS             complement(9880..10161)
                     /locus_tag="ACFOF2_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC7107812.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(10240..10374)
                     /locus_tag="ACFOF2_001523"
                     /pseudo
     CDS             complement(10240..10374)
                     /locus_tag="ACFOF2_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(10373..10831)
                     /locus_tag="ACFOF2_001524"
     CDS             complement(10373..10831)
                     /locus_tag="ACFOF2_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107813.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(11395..11598)
                     /locus_tag="ACFOF2_001525"
     CDS             complement(11395..11598)
                     /locus_tag="ACFOF2_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107814.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT         2778 a         3068 c         2895 g         3028 t
ORIGIN      
        1 aaacacacca aagacgacgg cagcgtcagt atgctgctgc ccgactttgt ccaactggtt
       61 caaagcgaag gcccggcagt cgtcaatatt caggcagccc ccgccccgcg cacccaaaac
      121 ggcagcagca atgccgaaaa cgattccgac ccgcttgccg acagcgaccc gttctacgaa
      181 tttttcaaac gcctcgtccc gaacatgccc gaaatccccc aagaagaagc agatgacggc
      241 ggattgaact tcggttcggg cttcatcatc agcaaagacg gctatattct gaccaatacg
      301 cacgtcgtta ccggcatggg cagtatcaaa gtcctgctca acgacaagcg cgaatatacc
      361 gccaaactca tcggttcgga tgtccaatcc gatgtcgccc ttctgaaaat cgacgcaacg
      421 gaagagctgc ccgtcgtcaa aatcggcaat cccaaagatt tgaaaccggg cgaatgggtc
      481 gccgccatcg gcgcgccctt cggcttcgac aacagcgtga ccgccggcat cgtgtccgcc
      541 aaaggcagaa gcctgcccaa cgaaagctac acacccttca tccaaaccga cgttgccatc
      601 aatccgggca actccggcgg cccgctgttc aacttaaaag gacaggtcgt cggcatcaac
      661 tcgcaaatat acagccgcag cggcggattc atgggcattt ccttcgccat cccgattgac
      721 gttgccatga atgtcgccga acagctgaaa aacaccggca aagtccaacg cggacaactg
      781 ggcgtgatta ttcaagaagt atcctacggt ttggcacaat cgttcggttt ggacaaagcc
      841 ggcggcgcac tgattgccaa aatcctgccc ggcagccccg cagaacgtgc cggcctgcgg
      901 gcgggcgaca tcgtcctcag cctcgacggc ggagaaatac gttcttccgg cgaccttccc
      961 gttatggtcg gcgccattac gccgggaaaa gaagtcagcc tcggcgtatg gcgcaaaggc
     1021 gaagaaatca caatcaaagt caagctgggc aacgccgccg agcatatcgg cgcatcatcc
     1081 aaaacagatg aagcccccta caccgaacag caatccggta cgttctcggt cgaatccgca
     1141 ggcattaccc ttcagacaca taccgacagc agcggcggac acctcgtcgt cgtacgggtt
     1201 tccgacgcgg cagaacgcgc aggcttgagg cgcggcgacg aaattcttgc cgtcgggcaa
     1261 gtccccgtca atgacgaagc cggtttccgc aaagctatgg acaaggcagg caaaaacgtc
     1321 cccctgctgg tcatgcgccg tggcaacacg ctgtttatcg cattaaacct gcaataaccc
     1381 acataattta accaaaatat tttttaaatg ataaaatgcc cgtcaatacc gattaacggg
     1441 cattttacaa aaatcagttg gttgatttta aacaaatatt ttttacgaca aaaaatattt
     1501 ccgcccattt tgccaacaaa acccaaaccg gaggcggaca gatacgctcc catccggacg
     1561 cttaataccc ccataaccat gcagctgatc gactattcac attcattttt ctcggttgtg
     1621 ccaccctttt tggcactggc acttgccgtc attacccgcc gcgtactgct gtctttaggc
     1681 atcggtattc tggtcggcgt tgcctttttg gtcggcggca accccgtcga cggtctgaca
     1741 cacctgaaag acatggtcgt cggcttggct tggtcagacg gcgattggtc gctgggcaaa
     1801 ccaaaaatct tggttttcct gatacttttg ggtattttta cttccctgct gacctactcc
     1861 ggcagcaatc aggcgtttgc cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg
     1921 aaaatgctga ccgcctgcct cgtgtttgta acctttatcg acgactattt ccacagtctc
     1981 gccgtcggtg cgattgcccg ccccgttacc gacaagttta aagtttcccg cgccaaactc
     2041 gcctacatcc tcgactccac tgccgcgcct atgtgcgtgc tgatgcccgt ttcaagctgg
     2101 ggcgcgtcga ttatcgccac gcttgccgga ctgctcgtta cctacaaaat caccgaatac
     2161 acgccgatgg ggacgtttgt cgccatgagc ctgatgaact attacgcact gtttgccctg
     2221 attatggtgt tcgtcgtcgc atggttttcc ttcgacatcg gctcgatggc acgtttcgaa
     2281 caagccgcgt tgaacgaagc ccacgatgaa actaccgttt cagacgctac caaaggtcgt
     2341 gtttacgcac tgattattcc cgttttggcc ttaatcgcct caacggtttc cgccatgatc
     2401 tacaccggcg cgcaggcaag cgaaaccttc agcattttgg gggcatttga aaataccgac
     2461 gtaaacactt cgctggtatt cggcggcact tgcggcgtcc ttgccgtcgt tctctgcacg
     2521 ctcggcacga ttaaaaccgc cgactatccc aaagccgttt ggcagggtgc gaaatctatg
     2581 ttcggcgcaa tcgccatttt aatcctcgct tggctcatca gtacggttgt cggcgaaatg
     2641 cacaccggcg attacctctc cacgctggtt gcgggcaaca tccatcccgg cttcctgccc
     2701 gtcatcctct tcctgctcgc cagcgtgatg gcgtttgcca caggcacaag ctgggggacg
     2761 ttcggcatta tgctgccgat tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt
     2821 atcccgtgta tgtccgcagt aatggcgggg gcggtatgcg gcgaccactg ctcgcccatt
     2881 tccgacacga ccatcctgtc gtccaccgga gcgcgctgca accacatcga ccacgttacc
     2941 tcgcaactgc cttacgcctt aaccgttgcc gccgccgccg catcgggcta cctcgcattg
     3001 ggtctgacaa aatccgcgct gttgggtttt ggcacgacag gcattgtatt ggcggtgctg
     3061 atttttctgt tgaaagataa aaaacgcgcc aacgcctgac ccctttccct gttcagacgg
     3121 catatgccgt ctgaagcttt ttggagcaaa ccgcaatgac cgtcccccat attccgcgcg
     3181 gccccgtcat ggcagacatt gccgccttcc gcctgaccgg agaagaaaaa caacgcctgc
     3241 tcgatcccgc cgtaggcggc atcatcctct tccgccgcaa cttccaaaac atcgaacagc
     3301 tcaaaacact caccgccgaa atcaaagccc tccgcacacc cgaactcatc atcgccgtcg
     3361 atcacgaggg cggaagggtg caacgtttca tcgaaggctt cacccgcctg cccgcaatga
     3421 gtacgctcgg cgagatttgg gacaaagacg gcgcgtccgc cgccgaaacc gccgccggac
     3481 aagtcggccg ggttttggca accgagcttt ccgcctgcgg catcgacttg tccttcacgc
     3541 ccgtcttaga tttggactgg ggaaactgcc ccgtcatcgg caaccgcagt ttccacagta
     3601 accccgaagc cgtaacccgc ctcgccctcg cccttcaaaa aggtttggca aaaggcggta
     3661 tgaaatcctg cggcaaacat tttcccggac acgggtttgt cgaaggggac agccatttgg
     3721 tgctacccga agacgggcgc agcctcgacg aactcgaagc cgccgacctc attcctttcc
     3781 gcattatgag ccgcgaaggt atggcggcgg ttatgcccgc ccacgtcgtt tatccacaag
     3841 tggacacaaa gcccgccggg ttttccgaaa tctggctgaa acagattttg cgccgcgaca
     3901 tcgggttcaa aggcgtgatt ttctcggacg atttgactat ggagggcgcg tgcggggcag
     3961 gcggcatcaa agaacgcgcc cgcatttcct ttgaggcagg ttgcgacatt gtcctcgtct
     4021 gcaaccgtcc cgaccttgtg gacgaactgc gcgatgattt ccgcattccc gacaatcccg
     4081 ctttggcgca acgttggcaa tacatggcca acacgctcgg cagcgccgcc gcgcaagccg
     4141 ttatgcagac ggcggatttc caagcggcgc aagcctttgt tgccgccctt gcctcgccgc
     4201 aggatacagc gggcggcgta aaagtcggcg aagcctttta aatccgcccg acacgcggca
     4261 aaaaggcaaa acggtatagg cacgccgtat tttttgcgct ataatcacgc cttacttaat
     4321 tcggaatgta gcgcagcccg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga
     4381 atcctctcat tccgaccaaa tccaaaccgc ctcggcatcc attgccggag cggtttttcc
     4441 ttttggcgtc aacagaaaaa tgccgtctga aacagcattc tgtttcagac ggcatttcgt
     4501 tttccctaac ccttctattt gcgtgcactg acaaagggta tcggcaaggt tcaaaagacc
     4561 atcggaattt aggaagccgg gcttgtaccc aacggcagca tcttattcag taaggctccg
     4621 tcctgattgg gcggtggaaa taccggcatt tccacgcaga agcggcacac tttccccgta
     4681 tgtcaaacat cggtaaaacg gtacgccggc cctttgagtt acactgctta aactactcta
     4741 ttcccgccca tttgtgcgtc tgcacgctca actgccaatg cacggatgcg tcggggcgac
     4801 tgtttaaaat accgatttgg cggatggtgt cgtagatgtt catcgcaccg tcttgctcac
     4861 agggcgaaag gtagtaatga tgtgcgcgga ttttgcgttc catgttttcg cagaacgcaa
     4921 ggacatcacc atcggcaaca atccgcactt cgtcggctgt ttcgatacag ctattttcat
     4981 atttggcggc gtagcaggct ttggggctgg tggcgacgta gtcgatttgc ggcggcgcgg
     5041 gattgagtcc gttggtttcg agacagagga aatagccttc cgccttgagc gtgtccagca
     5101 gcatatcgag atgcggctgt atggtcggtt cgccgccggt gatgatgatg ttgcgggcgg
     5161 cataggtttt cagacagcct aagatgtcgg acaggctcat catgccgaat gtcaaataat
     5221 cggtatcaca ccagccgcag gcgaggttgc atttgcccaa gcggacgaaa acggcgggca
     5281 tgcccgtgtt ccagccttcg ccttgcaggc tttcaaagat ttcgacgatg cggtattggg
     5341 gattttcggg ggcaacattg atttttttca taccgccaac accgccgccg ctgccaaaaa
     5401 tgcgggcaaa ccttgtttga cgagtatgcc tttgttgccg gaagagaacg cgccccacgc
     5461 ggcggcaatc aggacgaagc cgagaaacag taccgtcacg ccgtacaccg cgttatccgg
     5521 cgcagcaaac cgcgaccaca ccagcccgat gccgagaaag ccgttataca gcccttggtt
     5581 gccgaacagg gtctgcacgc gattttgttc cataaattca taaggcagct tgaacgtttc
     5641 cgccgctttt tcgctgggaa tctgtgtcat ttcaagccag gcgatgtaga aatgttcgac
     5701 ggcgacgaag aggactagga ggatgaagag gagtttcatg ggtaagcacc ttaatttgca
     5761 ctaagattta tatttaacaa actttaccaa ttttatttaa caaaaaagcc acccattatg
     5821 gatggcttct ctaccaaaac ttttcatgct acttttttca tggttgcagc ttttgcttta
     5881 taagctcgtt tttcaatttg tctttgaata gctgcaagtt gcttcgttaa tgcagcaatg
     5941 cgagcgaaac ggatatccat tgatttcata aataactcct tgctgttgtt aataaaggct
     6001 cgactgatta tttttgagca tagaacgatt ccgagaacat tcctcagtat ctttatttct
     6061 aacgttagta atcatagatt gtgcgtagag aagtccttga gccaaactaa tttttttgtt
     6121 ttccaaagaa aaatcattgg gactgatcgg aagtttatca acctcttgtt gtaaattttt
     6181 cacaatggat tccaaagtat ccaaatcagg atcaactaaa tcatgcatac catcaatccc
     6241 aaaatcatcg tacagtttca aaagaaattg tacgaactta ttaattacat ctatgctctc
     6301 ggcaataatg ccttcctctt gaatcatact actagttgta ttcatggtta ttatgcctga
     6361 aattttctat tacggtcgat tttttagaaa gtatggaatt ttacaattga ttatatgaca
     6421 agtcaaaaca aatctttaaa atttgtttca atattactcc ccttcatact ccgcacacga
     6481 tgtcggcgtt tcccacaatt tgacgcggca cacgttcagc cccgcgtttt tcagacggtc
     6541 gtacatttcg accgccatat tttcggcagt ggtgcggcag ggcaggcgca gggttttcat
     6601 gttccagccc tccaaaagcg cggcgatttg gcattcgcgg ctgttgccgc cgtggtagat
     6661 gaaggcgtgg tcgaaggggt cggtaatgtg ttgtttgaca atggctttca agtcggtaaa
     6721 gtccatcacc ataccgtctt tcgcgccgcc tttgataatg ccgtctgaaa cggtgatttc
     6781 gagtttgtag gtatgtccgt gcaggttttg gcatttgccg tcatgcccgt cgagcatatg
     6841 cgaggagtcg aaggtgaaga ttttggtgat tttcatgggt tctcaaatgg gcaaatagcc
     6901 ttgaaagccg atttcggcgg gtttgccgtg cggattcggg gtcaggcgga tatggaaatt
     6961 gccgatgcgg tcgagcgcaa gcaggttgtc cacgtctttt aagttgtctt tatgttgctg
     7021 aacatagctg tcggtcaaca gggtttgcag ggctttttgt ttggcttcgg caggcgtggc
     7081 ggcggcgaac agcccaaaag cgtgcgcttc ggcgagctgc cctgcgcgat aaccgccgac
     7141 attggcgaaa tacaggcgcg gggcgtgttc agacggcatt ttcgttttgg gcgtttcgga
     7201 tacggcgatg tcgtaaccgt ccgcccattc gacaacctgc cagccgtcga tgtggatttt
     7261 gtccgcatcg ccgaaccacg cggctttgag tgcggggacg gcttcacggt agtcgtcgca
     7321 cacggcaaat tggatgtcgt gcacttcgat attcgacctg ccggcattgc cgccgaggta
     7381 aaacatatgg agcttgggca tatcgtattc cttatggttt gcgcgtgttc agacggcctt
     7441 tttactttcc agatattccg ccagcccgcg ttcgcgcaag atacagctcg ggcattcgcg
     7501 gcagccgccg acgatgccgt tatagcaggt gtgggtttgc tcgcggatat agtccagcgc
     7561 gcccatttcg tccgccaacg cccaagtttg cgccttggtc agatacatca gcggcgtgtg
     7621 gatttggaaa tcatagtcca tcgccagatt aagggtaacg ttcatggatt tgacgaacac
     7681 gtcgcggcag tcaggatagc cggagaagtc ggtttcgcac acgcccgcga tgatgtgccg
     7741 tatcccctgc cctttggcgt aaatcgcggc atagagcagg aaaagcgcgt tgcggccgtc
     7801 tacaaaggta ttcggaacgc cgttttcggc agtttcgatg gcggcggtgt cgtccatcag
     7861 ggcattgtgc gtaatctgcc gcatcaggct caagtcgagt acggtttgtt tgacgcccaa
     7921 atcctgcgca atccagctgg cacgttccag ctcgacggca tggcgttgcc cgtattggaa
     7981 agtaatggct tggacgtttt cgcgcccgta gatttggatt gcctgaatca ggcaggtggt
     8041 cgaatcctga ccgcccgaaa agatgaccaa ggctttttgg tttgacatat cgaatcctag
     8101 ttttggtaac gcgggaggtt tgcgaaccgc gaagttggac agtaaagacg cggattatac
     8161 aggcaaacgc gttcagacgg catctgaacg ccatgccgtc tgaacacgcg gcttatgccg
     8221 acttgacggc gagttccgcc aagcgtttgc tttgtgcgaa ggcgatattg ttttcttcgg
     8281 cggtcaggac gggagccgct gtcgggaata tcttttttga cggcttcgca gacggcggag
     8341 actttgggga cggtgcgcaa tacggcttcg caaccttcaa cgctgtcgat gccgcgagcg
     8401 attcggagtg cgggattgcg ggtgctgccg ttttggaaat agtagaggac gaggattttg
     8461 agggggattt gggttcatgg gcggattccg ttaaaatggt tcggtcttca gacggcataa
     8521 cagttgttcc gatgccgtct gaaacagccg cagttcaggc atttgttcct gacggcaaaa
     8581 ttggctataa catcgggcat caatgcccaa cttactgacc gggcaagaaa ttatgacctt
     8641 tttacaacgt ttgcaaggtt tggcagacaa taaaatctgt gcgtttgcat ggttcgtcgt
     8701 ccgccgcttt gatgaagaac gcgtaccgca ggcggcggca agcatgacgt ttacgacgct
     8761 gctggcactc gtccccgtgc tgaccgtgat ggtggcggtc gcttcgattt tccccgtgtt
     8821 cgaccgctgg tcggattcgt tcgtctcctt cgtcaaccaa accattgtgc cgcagggcgc
     8881 ggacatggtg ttcgactata tcaatgcgtt ccgcgagcag gcgaaccggc tgacggcaat
     8941 cggcagcgtg atgctggtcg ttacctcgct gatgctgatt cggacgatag acaatacgtt
     9001 caaccgcatc tggcgggtca attcccagcg tccgtggatg atgcagtttc tcgtctattg
     9061 ggctttactg acgttcgggc cgctgtcttt gggcgtgggc atttccttta tggtcggctc
     9121 ggtacaggat gccgcgcttg cctcaggtgc gccgcagtgg tcgggcgcgt tgcgaacggc
     9181 ggcgacgctg accttcatga cgcttttgct gtgggggctg taccgcttcg tgccaaaccg
     9241 cttcgttccc gcgcggcagg cgtttgtcgg ggctttggca acagcgtttt gtctggaaac
     9301 cgcgcgctcc ctcttcactt ggtatatggg caatttcgac ggctaccgct cgatttacgg
     9361 cgcgtttgcc gccgtgccgt tttttctgtt gtggctgaac ctgttgtgga cgctggtctt
     9421 gggcggcgcg gtgctgactt cttcactctc ctactggcag ggagaagcgt tccgcagggg
     9481 cttcgattcg cgcggacggt ttgacgacgt gttgaaaatc ctgctgcttc tggatgcggc
     9541 gcaaaaagaa ggcaaagcct tgcctgttca ggagttcaga cggcatatca atatgggcta
     9601 cgacgagttg ggcgagcttt tggaaaagct ggcgcggtac ggctatatct attccggcag
     9661 acagggttgg gtgttgaaaa cgggggcgga ttcgattgag ttgaacgaac tcttcaagct
     9721 cttcgtttac cgtccgttgc ctgtggaaag ggatcatgtg aaccaagctg tcgatgcagt
     9781 aatgatgccg tgtttgcaga ctttgaacat gacgctggca gagtttgacg ctcaggcgaa
     9841 aaaacagcag caatcttgag tatttttgaa actgtatttt tatcctaaag acattttcct
     9901 atctgctgat tccaccgttt cctttctgtg tgccgccata atttttatga tagacaagcc
     9961 attcaagttt gcattgactg ctttttcatt ggcaatatcc aaatggctgg tcgctgcatc
    10021 taaaaatagg attttcggtt cgcaatataa ggcccgcgcc aatacgatgc gttgtttttg
    10081 tccgcctgac agtgcgctgc ccatatcgcc gatcaaggtt tcatagccca taggcatggc
    10141 ggagatttct tcgtgaatca tagcaaggca ggcgcaatgt tcgatttttc ctctatccgg
    10201 ttccgtatcg aaaaaacaag gggctgtact agattagccc taaatcccac accaatcccg
    10261 caggatttta agctgttgag acggtgtgcc gaagttaaat cgaaattcgc attctttcaa
    10321 gaacagcggg aaagatttac gatcgattcc gttgtatttt cgcaagacgc gtttagtcta
    10381 gagtctgtat attacattta tttttagggt ctgctagcca atttcttgtt cccttcatta
    10441 ttttatcttc tgaaagaaaa ttattttttt ccatgctatt aatattaatg atatgatttt
    10501 tatttaaaat aatttttcca tataaaattt ccggtttgta atagcaagag tctgaagcaa
    10561 atccttgccc gtcaatataa gaaaaataaa tgttatcatc ctccttataa tattctaaaa
    10621 ccaatccgga tattatttta tcaaaattcc cttcatttcc atctttcatg actaaaatat
    10681 aattagcagg ccaaccttta tcgtaaacat attcaaactg atagttcccg aaactctcga
    10741 tatccgaact aaaaaagaag aaaagcaaat taatagccaa tacaaaaaca aataagaaca
    10801 aaaatagcaa aattttcaac ttagttaaca atatttacct ctcctttaaa ttcaatcctg
    10861 aaaggtaccc cttaccctgg gctaccaatt atagtttcca tatttctaaa tattgttttt
    10921 acattacttt tttctccccc caaaggaatg cattttaaaa tcatgctttt caggtgctaa
    10981 tcgatactta ccattaacat ctttaatcac agatatattt ccatgtatag cccaacgtga
    11041 aaaatctgag tattatatac agttatacct tactgaaaca aagctagctg tacatatctg
    11101 taccatattt ttactacaaa caaaaaatat ttataattat taaggataag ataaaaaccc
    11161 ccaacgctag attaattctt aataatttca aataaaaaat atttgagatt ttttcttttc
    11221 caaataatat attgaaatat ttaaagttaa ggtagcttga tttaagtaga aaatcaaaaa
    11281 aaccattaat taatttttct tctttaaata ttttatatga tattataaaa ttaaataaca
    11341 ttgcgaaaac atatgaaatc atcaaaatac caaatatata gtgggaatgc attattattt
    11401 agctccttct tttggctctg acaaattgac agtccattga tatccaatgc ttgcatttgc
    11461 aattgctgac gcacctgccc ctgttttccc tgttagtatc aatggttctt ttcttgcaag
    11521 atgtgcacct gcacaaccac ctaatactaa accaccacct attgtaaatc cttcctgttg
    11581 tatctctaat gataacattt tcttcttgtg ccgcccttaa tacagattgt ggaattggat
    11641 tgacctgagc aggaacttct aaaataagtt ttccttcaag ctgtcttcct cttagcgaag
    11701 caggtgtact tggaacattg gaaatgacaa agtgagtgcg caaagccacc tgaaggcgat
    11761 gtgtttgaa
//
LOCUS       DAXIHS010000065        11603 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXIHS010000065 DAXIHS010000000
VERSION     DAXIHS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.065"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(238..642)
                     /locus_tag="ACFOF2_001526"
     CDS             complement(238..642)
                     /locus_tag="ACFOF2_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107815.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKFNGIPKAHF
                     ELYLKGYERRFNNSEIKVQISILKQLVKSSLS"
     gene            complement(948..2207)
                     /gene="rho"
                     /locus_tag="ACFOF2_001527"
     CDS             complement(948..2207)
                     /gene="rho"
                     /locus_tag="ACFOF2_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC7107816.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2427..4811)
                     /gene="ppsA"
                     /locus_tag="ACFOF2_001528"
     CDS             complement(2427..4811)
                     /gene="ppsA"
                     /locus_tag="ACFOF2_001528"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC7107817.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPMLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5223..6044
                     /locus_tag="ACFOF2_001529"
     CDS             5223..6044
                     /locus_tag="ACFOF2_001529"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC7107818.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(6283..6945)
                     /locus_tag="ACFOF2_001530"
     CDS             complement(6283..6945)
                     /locus_tag="ACFOF2_001530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC7107819.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPDHTDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(7006..7971)
                     /locus_tag="ACFOF2_001531"
     CDS             complement(7006..7971)
                     /locus_tag="ACFOF2_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFC7107820.1"
                     /translation="MSHHHEHHHHDHSHAHTHTANKKVLRVSFIIIAAFMLLEAFGGW
                     LTHSLALLSDAGHMFSDAFSLGVALWAFKLGEKETTLQKTFGYKRFEILTAMFNGLSL
                     VVIAVLIFYEAVKRLLYPPEIATLGMLVISVVGLLVNIGVAVYMLKNSDTEANVNMRG
                     AYLHVLSDLVGSVGAIAAAVLMMAFGWQWADPLASVFVAALVGRSGWSLLKQTLHILM
                     EGAPENIHTDDLLAVIRNTDGVKSVHDLHVWTITSNINVLSCHIVVDGSMTVAESEQI
                     AYRIEHELSHKNIGHCTIQIESERHPHPDSVLCANETDGAHHHHH"
     gene            complement(8017..8844)
                     /locus_tag="ACFOF2_001532"
     CDS             complement(8017..8844)
                     /locus_tag="ACFOF2_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC7107821.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQSKKVV"
     gene            9274..9897
                     /gene="lolA"
                     /locus_tag="ACFOF2_001533"
     CDS             9274..9897
                     /gene="lolA"
                     /locus_tag="ACFOF2_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC7107822.1"
                     /translation="MMKPHNLFKFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            10170..11309
                     /locus_tag="ACFOF2_001534"
     CDS             10170..11309
                     /locus_tag="ACFOF2_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7107823.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            11442..>11603
                     /locus_tag="ACFOF2_001535"
     CDS             11442..>11603
                     /locus_tag="ACFOF2_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC7107824.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT         2619 a         2908 c         3258 g         2818 t
ORIGIN      
        1 gggcagtcct tccaacaacg gcgacaggtg tttgcactca tgggcattgg cggaggtata
       61 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa cagtacggaa
      121 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
      181 acgctgtact ggtttttgtt aatccactat aaaaatttat ggggctgtcc tagataacta
      241 ggataaactc gattttacta attgttttaa aatggaaatt tgaactttta tctcactgtt
      301 gttaaaacgc cgttcgtacc cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt
      361 aaacttgcgt aaatgacgtt ttgcccgatt ccaaaagttc tcaattccat taatatggtt
      421 ttgtcgttca gcaaaataac tttcatctgc ttctacttcg ccatcaaaca tttccaaatg
      481 tggactgttt tgataaataa gtaatcgtaa acgatgaaaa taataggctg cggtactttt
      541 attaacgcct actaactctg ctgctgttct tgcagttaca cctgcgacaa acagttcaat
      601 gagtttattt tgtttatact ggcttagacg actttttctc ataggggcaa ctctaactta
      661 atttgaattt ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc
      721 ggcgcaacct tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag
      781 ccggttttgc aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc
      841 gttaacgggg ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc
      901 gattttcagg cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc
      961 atcagttcaa agaaatcgtc gttgttttta gaggctttga ttttcccgtt taagaactcg
     1021 gtagcttcga tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgtaac
     1081 tggtcgttcg gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg
     1141 gggaagaggc gtttttccgc catacggcgg tcgaggtgca attccatatt gccggtgcct
     1201 ttgaattctt cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgcggtggca
     1261 atgatggtca gcgaaccgcc ttcttccacg ttgcgcgctg cgccgaagaa acgtttgggg
     1321 cggtgcagcg cgttggcatc gacaccgccg gtcaggattt tgcccgaggt ggggacgacg
     1381 gtattgtagg cgcgggcaag gcgggtaatc gaatccagca ggatgaccac gtcttttttg
     1441 tgttctacca tacgcttggc tttttcaagc accatttcgg caacttggac gtggcgttgc
     1501 gccggttcgt caaaggtgga ggaaaccact tcgccgcgga cggaacggct catttcggtt
     1561 acttcttcgg gacgctcgtc aatcaagagg acgatgagct cgacttcagg atagtttgcg
     1621 gtaacggcgt gggcaatatt ttgcaacatc acggttttac cgcttttggg cggggcaacc
     1681 aagagggcgc gctgaccttt gccgatgggg gaaatcagat cgatggctcg tccggtcagg
     1741 ttttcttcgg actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc
     1801 aggttttcaa acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggaatcc
     1861 agtctgacta gggcaaaata gcgttcgttg tctttgggga cgcgcacgct gccttcgatg
     1921 gtgtcgcccg tatgcaggtt gaagcggcgg atttgggtgg gcgagacata gatgtcgtcg
     1981 gggcccgcaa gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt
     2041 tcgagcgtgc cggagcaggt gaaaccctcg ccttttttca tcatttggcg gacgatggca
     2101 aatacgaggt cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat
     2161 tctaagagtt tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat
     2221 tttgaagagg aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt
     2281 gctcggggaa gggggaattg taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg
     2341 gggcatcggc aggaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt
     2401 ttcaggctcg ggggtatggg cattgattat ttgttcaatt cattcgccaa atatagccaa
     2461 gtttcgatga cggtatccgg gttcagggaa acgctttcga taccttcctc aaccagccat
     2521 ttggcgaagt ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc
     2581 ttgcggcagg cggagatggc aaggtgcagc atcactttga cggcagggtt gcgttcgtca
     2641 aacgattcgg ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg
     2701 ttcgagccga tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg
     2761 ctcggcagct cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt
     2821 tctttcaggg ctttgacaac ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg
     2881 atttcaacgt tggtcaaccc catttcatcg cggacgcgtt tcaaggcttt gcattccaag
     2941 gcgaaacagt ctttgaagtt gtcggcgaca taacgcgccg caccacggaa gcccaacatc
     3001 gggttttctt catgcggttc gtatacgctg ccgccgacca ggttggcgta ttcgttggat
     3061 ttgaagtcgg acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc
     3121 acgccttcgg cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg
     3181 cgggtaattt ccgcttttaa ttcgtcatct tgtttgtcaa attccaacaa ggctttcggg
     3241 tggataccga tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg
     3301 ggcaggttgg cgaagctgaa tgcgagttcg gggttgccga cgttcatcat gactttgacg
     3361 ggtgctttgg gcatattgtc caaggcgaca tcggtaattt gtacgtccaa cagaccggca
     3421 tagatgaagc cggtatcgcc ttcagcacag gatacggtaa cttcttgacc gtttttcagc
     3481 aattcggttg cattgccgca gccgacaacg gcaggaatgc ccagttcgcg cgcgataatc
     3541 gccgcgtggc aggtgcgtcc gccacggttg gtaacgatgg cggaagcccg tttcatcacg
     3601 ggttcccaat ccggatcggt catgtcggta accagtacgt cgccggcttc gacggaatcc
     3661 atctcggaag catctttaat caggcgtact ttgccctgtc cgactttctg accgatggca
     3721 cggccttcgc ataatacggt tttttcgccg ttgatggcga agcggcgcag gttgcggctg
     3781 ccttcttcct gggatttgac ggtttcggga cgggcttgca ggatgtagag tttgccgtcc
     3841 aagccgtcgc gtccccattc gatgtccatc gggcggccgt agtgtttttc gatggtcagt
     3901 gcgtaatgcg ccaactcagt gatttcttcg tcggtaatgg agaagcggtt gcggtcttcc
     3961 tcggggacat cgacgttggt tacggattta ccggcttctg ctttgtcggt aaaaatcatt
     4021 ttgatgtgtt tcgaacccat ggtcttgcgc aggatggcgg gcttgcccgc tttgagcata
     4081 ggtttgaaca cataaaattc gtccgggttg accgcacctt gtacgacgtt ttcgcccaga
     4141 ccgtaagagg aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac
     4201 atcacacctg atgcgccgct gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg
     4261 acgatgtcgt gttcgaagcc tttgtggacg cggtaggaaa tggcgcggtc gttgtacagg
     4321 gaggcgaata catggtgcat cgcttcttta acgttttcca atccgttgat gttcaagaag
     4381 gtttcctgtt ggccggcaaa tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt
     4441 acggcaacgg aaatgtccgc accgccggca tcggcaacca ttttgttcca tgccgcttcg
     4501 atttcggcat cgagctgttc ggggaagggc gtgtccaaaa tccattggcg gatttctttg
     4561 ccgacgcgtg ccagttcggc aacgtcttcg acatctaatt ttgccagtgc ggcggaaatg
     4621 cgttcgctca gaccgttgtg tgcgaggaat gcgcggtagg catcggctgt ggtggcaaag
     4681 ccgccgggga cgcgaacgcc tttttcggtc agctgactga tcatttcgcc cagcgaggcg
     4741 tttttaccgc ccacgcgttc aacatctgtc atacgcaggt tttcaaacca gattacgtag
     4801 ttgtcggcca tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct
     4861 tattttaagc gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc
     4921 cgtctgaaaa attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatgttt
     4981 ctttttttgg gtattttctt tgtaaaacag atggtttgaa taggttaatg ttttttctgt
     5041 ttgatttttt gtttattttt ttaaaatttt atgccaaaaa acactttatc taaataattt
     5101 tatttcaaaa taatattgtg tctgtttggg tgtaatccga ggtaggtgtg ctgcgggtgc
     5161 tttccttgtg tctgctgctg ctgttatgat gagattttaa acctgtgttt taaggatgga
     5221 agatgagcag tccgcgccat gtgttttaca tttccgaccg taccggtctg actgccgaga
     5281 atatcggcga ggctttgctg aaccagtttg gcaatctgtc gttcaaacgc catacgcatc
     5341 cgtttgtcga tacgccggaa aaggcgcgcg cggtggtgga gaaggtcaat cggagccggc
     5401 aggaaaacgg tcagcgtccg attgcgtttg tcagtgtggt tgatgacgaa atccgtcgga
     5461 ttatcaaagg ggcggatgct tttcagatta atttctttga gacttttttg ggactgttgg
     5521 agaaagaact caataccgaa gccacggcat ccgggcaggg gcatcacagt atcggtaata
     5581 cgaagcgtta cgatgcacgt atggaggcgg tgaatttctc attgaaccac gatgacgggg
     5641 tcagcgataa gaaccttcag gaagcggatg taatcttgat gggtgtatcg cgttcgggca
     5701 aaacgccgac ctgcctttac ctcgccctgc aatacggcat ccgtgcggca aactatccgc
     5761 tgattcccga cgatttggaa tcggccgatc tgccgcgtat ggtcaagcct tatagggata
     5821 agctgttcgg gttgaccatc cagccggaac gtttgcaggc catccgccaa gagcgccgcc
     5881 cgaattcaac ttatgccaaa atcgatacat gccgcagcga ggtggcggac gcgcagagta
     5941 tgttcagacg gcatggcatt ccgtttgcga atacgacgga taaatcagtg gaagagcttg
     6001 ccgtacacat ccttcaggcg tgcaagctca aacgcaggtt ttgacgggct ttgattcggt
     6061 ttgaaggtgg aaatgccgtc tgaaatcagg tttcagacgg cagttttata gtggattaac
     6121 aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggaa ccgattcact
     6181 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgctgtact
     6241 ggtttttgtt aatccactat atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     6301 cagacattcg agcagatcga gtggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     6361 atcgtcttcg ggagcgatgt agccccattc ggcgaggacg gttttcatgc cggcgttgcg
     6421 gcctgcttgg atgtcgcgtt cggcatcacc gacgtagagt gtgtgttgcg ggtcggcgtg
     6481 gatttgtccg cacgcataca gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc
     6541 gccgctgacg acgacggcgg gtgggatgat gaagccgagt ttggggacga gtttgtcggt
     6601 gaagcgcata ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgt cgagttcggc
     6661 gatgagttcg tttacgccgc cgaagagggt ggtgtcttgg gcgtagcggc tgtcgtattc
     6721 gtcaaggtat tcggtgcgcc atcgggcata gtcggtgtgg tcgggggtga tgcctgcgcc
     6781 gagtttgagc agacctgccg cgccgtggct ggcttggttg cggatttcgt ccatgctttt
     6841 gggcggcagt ccgtggcggg cgagcagggt attgagtgcg ccgccgaggt ctagggcggt
     6901 gtcggcgagc gtgccatcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttggt
     6961 ttttcgatgg ggtcgtctga aaagggtttc agacgacctc tgggtttagt ggtggtgatg
     7021 gtgtgcgccg tcggtttcgt tggcacataa gacgctgtcg ggatgcgggt ggcgttcgct
     7081 ttcgatttgg atggtgcagt gtccgatgtt tttgtgggac aactcgtgtt cgatgcggta
     7141 ggcgatttgt tcggattcgg caacggtcat gctgccgtcc acgacgatgt ggcaggataa
     7201 tacgttgata ttgctggtaa tcgtccaaac gtgtaagtcg tggacggatt tgacgccgtc
     7261 ggtgttgcgg ataacggcga gcaggtcgtc ggtgtggatg ttttcgggtg cgccttccat
     7321 caggatgtgc agcgtttgtt tgagcaggct ccagccgctg cgtccgacca acgcggcaac
     7381 gaacacgctg gcgagcgggt ccgcccattg ccagccgaac gccatcatga gtacggcggc
     7441 ggcaatcgcg ccgaccgagc cgacaaggtc gcttaaaacg tgcagatacg cgccgcgcat
     7501 gttgacgttg gcttcggtgt cgctgttttt cagcatataa accgccacgc caatgttgac
     7561 cagcagcccg acgacgctaa tcacgagcat gcccagcgta gcgatttcgg gcggataaag
     7621 caggcgtttg acggcttcgt agaaaatcag cacggcgatg accaccagcg acaagccgtt
     7681 gaacatggcg gtgaggattt cgaaacgttt gtagccgaag gtcttttgca gcgtggtttc
     7741 tttttcgccg agtttgaacg cccacaacgc cacgcccaag gaaaatgcgt cgctgaacat
     7801 atgccccgcg tcggaaagca gcgcgagcga gtgggtcagc cagccgccga acgcttccag
     7861 cagcataaag gcggcgatga tgatgaacga cacgcgcaat acttttttgt tggcggtatg
     7921 cgtgtgggcg tgggaatgat cgtgatgatg gtgttcgtgg tggtgagaca taatgttcct
     7981 caacagaggt cgtctgaaaa tccggattcg gggttttcag acgacctttt tgctttgttt
     8041 caaaccctgc tcgaaatctt ccaacatatc caattcgaag cggctgaagc ccgctttttc
     8101 gcgcgcttcg atgttcacat agccgcggaa gataaacata tcgtaacggg caatcagact
     8161 gcggaacagg gcgacaggtt ccaaaccgcg ttcgcggcag aggtgttgat accaatggtt
     8221 gccgatggcg acatgaccca cttcgtcgcg gtaaatgatg tccaatacgc cgcaggtttc
     8281 cgaatcgccg cgctgcgcca ccttcgcgcg tatccccggc gtaacgtcca gcccgcgcgc
     8341 ttccaacacg cgcggcacta aagccatacg caacaaagga tcgtaggcgg ttttgtatgc
     8401 catatcccat aaatgattgt gcgcctcgaa atcgccgtaa tcgaagccga aagcgcgcag
     8461 cctttcgcgc atcaggcgga agtggtacac ctcttccttc gccactttca cccagtcgcg
     8521 gacaaactga aacggcagcg tgcggaaacg gtatgccgcg tccaaagcca gattgacggc
     8581 gttgaattcg atatgcgcaa tcgcgtgcag catcgccgca tagccttctg ccgtgttcat
     8641 tttgcgcggg gtcagttgcg acggcgcgac caaaacaggc ttgtccggcc gccctgcgcg
     8701 ggggaaatcc agcggcggcg tgtttgtttc agcaccgtcc atgttctgaa cggcggcaaa
     8761 cgcctcatcc gtcagccgtc ctttttcatc tgggtcgccc gaaagcaggg cgcgttccag
     8821 caaagcataa atatcaagtt tcatctcaag tccgccgtgt tcggaaaaca aatattatag
     8881 cgttttaaaa aaacaagatg aggcatataa tctccgcgat tcggcattcc gcgcccaaac
     8941 cgtcaaatat agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag
     9001 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatc tgtactgtct
     9061 gcggcttcgt cgccttgtcc tgatttttgt taatccacta taacgcggca cacattaaag
     9121 ggcagcgtgg cgcgcctgcc ttttccgagc ggtcaaaaaa atcagccctc ggaaaacgcg
     9181 gtttgtaaaa tgcaaaccgc ccgtaacgcc gcccgtatga ttgttttgcc gcgccgatac
     9241 tttacgccac acccatccca acaaggaaaa ataatgatga aaccgcacaa cctgttcaaa
     9301 ttcctcgccg tttgctccct gaccgtctcc gtcgcttccg cacaggcggg cgcggtggac
     9361 gcgctcaagc aattcaacaa cgatgccgac ggtatcagcg gcagcttcac ccaaaccgtc
     9421 caaagcaaaa agaaaaccca aaccgcgcac ggcacgttca aaatcctgcg cccgggcctc
     9481 ttcaaatggg aatacacttc gccctacaaa cagactattg tcggcgacgg tcaaaccgtt
     9541 tggctctacg atgtcgattt ggcacaagtg accaagtcgt cccaagacca ggccataggc
     9601 ggcagccccg ccgccatcct gtcgaacaaa accgccctcg aaagcagcta cacgctgaaa
     9661 gaggacggtt cgtccaacgg catcgattat gtgctggcaa cgcccaaacg caacaacgcc
     9721 ggctaccaat acatccgcat cggcttcaaa ggcggcaacc tcgccgccat gcagcttaaa
     9781 gacagcttcg gcaaccaaac ctccatcagt ttcggcggtt tgaataccaa tccccaactc
     9841 tcgcgcggcg cgttcaagtt taccccgccc aaaggcgtgg acgtgttgag caactgatgc
     9901 cgtccgcccc gatgccgtct gaaagccgcc gaggcttcag acggcatttt cacgcaggcg
     9961 gaacaatgtc ccgcattacc gcccgatcgg gcatcggaac ggcaaacccg tgaaaattaa
    10021 cggttgcgcc ccggctgttt ttgccgttta atgcaaacct tgctgcacca agggccaaga
    10081 aagccgaccg gccgccccca cagcttccga tgcaggcggc ccggccgtcc ctgcaatgtt
    10141 ttttattttt gaacgaaagg tcgaaaacca tgaaaaaaac actggtggcg gcggcaatcc
    10201 tgagcctcgc cttgactgcg tgcggcggcg gaagcgatac cgccgcccac cccccctccg
    10261 ccaagcccga agccgaacaa tcgggcaaac tcaatatcta caactggtcg gattatgtcg
    10321 atcccgaaac cgtcgccgcc tttgaaaaag aaaccggcat caagacgcgt tccgattatt
    10381 acgacagcaa cgaaacactg gaggcaaaag tcctgaccgg caaatccggc tacgacctga
    10441 ccgcgccgtc catcgccaac gtcggccggc aaatcaaagc gggcgcgtat cagaaaatcg
    10501 acaaggcgca aatcccccat tacggcaaca tcgataaaga tttgctgaaa atgatggaag
    10561 ccgtcgatcc gggcaacgaa tacgccgtcc cctatttctg gggcatcaac accttggcaa
    10621 tcaacacgcg gcaggtgcaa aaggcattgg gtacggacaa gctgcccgaa aacgaatggg
    10681 atttggtgtt caaccccgaa tacaccgcca aactcaaatc ctgcggcatc agctatttcg
    10741 acagcgcaat cgaacagatt cccttggcgt tgcactattt gggcaaagac cccaacagtg
    10801 aaaatcccga agacatcaaa gccgccgtcg atatgatgaa agccgtccga ggcgacgtga
    10861 aacgcttcag ctcttccggc tatatcgacg atatggcggc gggcaacctg tgtgccgcca
    10921 tcggttacgg cggcgatttg aacattgcca aaacccgtgc cgaagaagcc gcaaacggcg
    10981 tggaaatcaa agtattgacc ccgaaaaccg gcgtgggcgt gtgggtggat tcctttatga
    11041 ttccgcgcga cgcgcaaaac gtcgccaacg cccaccgcta tatcgactac acgctccggc
    11101 ccgaggtggc ggcgaaaaac ggcagcttcg ttacctacgc gcccgccagc cgtcccgcgc
    11161 gcgagctgat ggatgaaaaa tacacctccg acgcatcgat tttcccgaac aaagaactga
    11221 tggaaaaaag tttcatcgta tcgcccaaat ccgcagaatc cgccaaactg ggcgtgaagc
    11281 tgtggcaagg gctcaaggcc ggaaaataac cgcaacccct gccgtctgaa acctttcggg
    11341 cggcaggaaa cggcgcgtcc gttatcaaac aggggggcgt ttcccctcct gccggttatg
    11401 attgggttaa gattaaaatg atttagtaaa atgagaaaga tatggattta agtatcgtag
    11461 ttcccattta taatgtcgaa agttatttgg aagcgtgttt aagttccata gaacctatat
    11521 taagtaatga aaatgtcgaa cttattcttg tgaatgacgg gtcaaaagac ggaagtgaag
    11581 atatatgtta taaatatata gat
//
LOCUS       DAXIHS010000066        11176 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXIHS010000066 DAXIHS010000000
VERSION     DAXIHS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.066"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            122..304
                     /locus_tag="ACFOF2_001536"
     CDS             122..304
                     /locus_tag="ACFOF2_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107825.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(366..1367)
                     /locus_tag="ACFOF2_001537"
     CDS             complement(366..1367)
                     /locus_tag="ACFOF2_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC7107826.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1444..5277)
                     /locus_tag="ACFOF2_001538"
     CDS             complement(1444..5277)
                     /locus_tag="ACFOF2_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC7107827.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKVRYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5538..5634
                     /gene="ffs"
                     /locus_tag="ACFOF2_001539"
     ncRNA           5538..5634
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFOF2_001539"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5802..7493
                     /gene="dld"
                     /locus_tag="ACFOF2_001540"
     CDS             5802..7493
                     /gene="dld"
                     /locus_tag="ACFOF2_001540"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC7107828.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7625..7732
                     /locus_tag="ACFOF2_001541"
                     /pseudo
     CDS             7625..7732
                     /locus_tag="ACFOF2_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7788..8159
                     /locus_tag="ACFOF2_001542"
     CDS             7788..8159
                     /locus_tag="ACFOF2_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC7107829.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8314..8862
                     /locus_tag="ACFOF2_001543"
     CDS             8314..8862
                     /locus_tag="ACFOF2_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC7107830.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8996..10087
                     /locus_tag="ACFOF2_001544"
     CDS             8996..10087
                     /locus_tag="ACFOF2_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC7107831.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10561..10731
                     /locus_tag="ACFOF2_001545"
     CDS             10561..10731
                     /locus_tag="ACFOF2_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC7107832.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2626 a         3060 c         2992 g         2498 t
ORIGIN      
        1 tttgtactgt cttcggctcg ccgccttgtc cttatttaaa tttaattcac tatatttgcc
       61 cgtgcatttg tgataaattc ggcactgtat ttgatatgat tttaacaaat cgggaggggc
      121 aatgttttgg tatgtaatcg gcttttgctc ctttgtcgtc gcgctgctgt cgctgtgggt
      181 caatgtcggc gcattcggta tgcaggaaga cgataccccg caatcggatt atgaaaaacg
      241 tttgggcttg ggtgcgaagc tgaaaaacaa aaatacgccg gatacggaag aaaagcagca
      301 ataaagatat gaaatgccgt ctgaaagatt ttcagacggc atttgctttg aagcatggga
      361 gacagtcaaa catttatctc atgctcaaat gcgttcagga ttcggcggac ttcatccgca
      421 tccttaaccg ttcggacgcg gtcaaacagc gtttgcgccg ggtcgaacgt ttttttcatc
      481 atgcccaacc attgcttaag acgggcgatc gggtatttat tgtttgcctc ttttgtcagg
      541 cacagctcga aaaactgccg tatccatttg gaaacttcgg caaaatccgt gtctttgacc
      601 ggcccgccgt tctcgtattg cttgatttgc cgcgccaaat cggggcggat gaccgcgccg
      661 cgaccgagca tcacgctgtt gcagccgctg attgttttga tgccgatata gtcttgcagg
      721 ctgaaaacgt cgccgttggc ggtaacggga atattgacgc tgtctcggat tttccttatc
      781 cattcccaat gcgccggcgg ttcgtaaccc tcggctttgg tacgcgcgtg tacggtcagt
      841 ccgcatgcgc ccccttcggc aatcgcacag gcgcattcca aagcccggct tttgtcttcg
      901 taaccgagcc gcatttttgc ggtgagcgga atatgtgcgg gcaaacgtcc gcgcagcgtt
      961 ttgacgatgt ggaatatcag ttccggctct tttaaaagga ttgcgccgcc tttgtgtttg
     1021 ttgacggtgg gcgcggggca gccgaagttc aaatcgattt tgttcgcacc gaagcggacg
     1081 gcttccagcg cattcgccgc catattgtcc gcatcgctgc ccaaaagttg gacggtgcag
     1141 ggcgtgccgg aaaacgtttt gtttccgttg gcgatttcgg ggacatattt taaccatatg
     1201 gatcgggaat gcacggtatg ggtaatgcgt acaaattcgc tgacgcattc gtcgtagccg
     1261 ccaatacgcg tcagcaggtc gcgcatcacg tcatcgacca gaccctgcat aggggcaagg
     1321 atgattcggg ttttttgttt gggttcggtt gtttgtccgt caatcatgat ttgggtaggt
     1381 attgggttta aacaatgccg tctgaaggct ttcggcgttc agacgacatt tccgtgtccg
     1441 ttgttacagc aatacttttt ccacgccgcc gttgttggct tttttcacaa actcgtctag
     1501 ccagttttcg ccgagaatgt gtttcgccat ttcgatgacg atgtagtcgg caggcatatt
     1561 gttgtcgtcg gcgtagcggc tcaaaccttg caggcaggcg gggcaggaag tcagcatttt
     1621 gacgggttcg ccctgcggca gctctttgag gtttttctcg atttcctctt gtttgcggaa
     1681 cttgacctga gtggcgatgt ctggccgttt gacggcaaac ataccggatt cgccgcagca
     1741 gcggtcgctt aaaacgactt tctgtcccat caggctgctg gccatttggg tggcgttcat
     1801 ggttttaatc ggggtgtggc aggggtcgtg gtagaggtat tgctgacctt tcacgccgtc
     1861 gagtttcacg cctttttcga gcaggtattc gtggatgtcg atgatgcggc agccagggaa
     1921 gatttcctcg aagcggtatt tttcgagctg gtcgtaacaa gtgccgcaac tgacaacgac
     1981 ggttttgatg tcgaggtagt tgagggtgtt cgccatacgg tggaaagcca cgcggttgtt
     2041 ggtgctcatt tcttcggctt tagccttgtt gccgcccgcg tcttgcggat agccgcaaca
     2101 catatagccg ggcggcagga cggtttgtac gccgacgtgc cagagcatgg cttgaacggc
     2161 aagtccgatt tggctgaaca ggcgttccga accgcaaccc gggaagtaga acacggcttc
     2221 ggcatcttcg ggcgcggcgg ggttgcggat gatggggatg cttttgccgt cttcgatgcc
     2281 caataaggag cgcggtgttt tagcgggtac gcttttgggc agtgggcggt tgatgaaatg
     2341 gataacctgt tctttaatcg gggctttgcc gacggttgcc ttgggttcgg ctttttgctt
     2401 tttcgtgccg atcggaagaa gcttgccgat tttgtaggca aagttttgcg cggggaagcc
     2461 ggtttgtatc atggcggcgc gaagggcttt gatggttttc gggccggtgg cgttcaaaaa
     2521 cgccataccc atagctgcgg caggcgcaaa tcgtttgtgg ccggaatcgg caaggtagtt
     2581 gcggacggct acggttacgt cgccgaagtc gatgttgacg gggcagggtt tgacgcagcg
     2641 gtggcacacg gtgcagtggt cgccgatgtc catgagttct tcaaagtgtt tgatggaaac
     2701 gccgcgacgg gtttgttctt cgtataagaa ggcctcggtc agcaaaccca cgccgaggat
     2761 tttgttgcgg gggctgtaca gcaggttggc acgcggaacg tgggtggagc agacgggttt
     2821 gcatttgccg cagcgcaggc agtctttgac ggaatcggca atcgtgccga ggtctgattt
     2881 ttccataatc agcgattccg cgcccaaaag ctcgaaagac ggcgtgtagg cgttgcgtaa
     2941 gtccgagcct ttcatcagtt tgtgacggtt gaaggtgtgt ttgggatcga cttggttttt
     3001 gtagtcccaa aacggctgca tttcttcgtc gcttaaaaat tcgagcttgg tgatgccgat
     3061 gccgtgttcg cccgaaatca cgcctccgag cgaacgggcg attttcataa tgcgttcgac
     3121 tgagcggtat gccgtctgaa gcatttcggc atcgtctgag ttaaccggaa tattggtgtg
     3181 aacgttaccg tcgccggcgt gcatatgcag ggcgacaaag acgcggctgc gtacggtttt
     3241 tgcgtggatt tttcccaagc cttggataat tttggtgtcg gttttgccgc tgaagatttc
     3301 agaaagcggc ttcattacgt ccgctttgac agacacgcgc aggcggaaat cgcggaatgc
     3361 ggtaaagcag ctttcattgt ctttggcttc gggcgcggcg tggacggctg agccatagcg
     3421 tactttatag tcggcaagcg gcgcgtctaa gttagcaagc agccattccc aacgcgcttt
     3481 gacggcggaa acgtgggcaa gggcgtgttt gccgcgttcg cccaaaagtt cggcggtcgg
     3541 caggtcggtt cccattttgt cgatggggag tttgccggac agatattgtt ccaaggcggc
     3601 acagagtttg agtttgtttt ggatggaaag ctcaatgtta atgcgttcga tgccgtctga
     3661 atactcaccg agcctttcga gtgggatgac cacgtcttcg ttaattttaa aggcgttggt
     3721 gtgtttggcg atggcggcgg tgcggctgcg gtcgagccag aaggttttgc gcgcttcggg
     3781 cgatacggcg ataaagcctt cgccgtcgcg ggcgcgggca agttcacaga tgtgctcggc
     3841 ggctgcctct acggcggctt cgtcgtctga aaccacgtcc gccagtaaga ccattttcgg
     3901 ccggcctttg cctgccgctt tggtggcgta gccgacggcg cggacataac gccagtccaa
     3961 atgttccaaa cctgccagcc gaacgctgtc gtgggcgagc aggaaatcgc ggatttcgac
     4021 gatagaaggc gtagcggtgg cgaccgtgcc gaaaaactcc atacacacag tgcgcgtgta
     4081 tttcggcatt ttgtgcaaca cgaaagcaac gccggtgatg atgccgtccg tgccttcttt
     4141 ctgtacgccg ggcaggccgc tcaagaattt gtcggtaacg tctttgccca aaccgacttt
     4201 gcgaaatttg tggccgggaa tttccaaacg ttcggtttta acgatattga ggccgtctga
     4261 atccagcgtg tgaacgtcga acacggcggt ttcttcgtcg tggattttgc cgaaattgtg
     4321 gcgcacgcgc tcgatgcgca gccattcgcc ctgcgggttg accatctgcc agtaggcgag
     4381 gttgtccaac gccgtccccc acagcacggc ttttttaccg cccgcgttca tcgccacatt
     4441 accgcccaca caggacgcat cggcggaagt cggatcgacg gcgaacacca aacctgcctg
     4501 atgcgcggtt tcttccaccc gccgcgtaac cacgcccgcg ccgcaccgga taatcggatg
     4561 cttgccgtcc aagcctgcca gctcaacgta ttcaacacca cgatgcttgt cgagtttttc
     4621 ggtattgatg actgcgctgt ttgcgtccaa aggaatcgcg ccgccggtat aacccgtgcc
     4681 gccgccgcgc ggaataatga ccaaatccag ctcgattaag gcgcgcacca aaggcgcgat
     4741 ttccgcctcc gtgtcgggat tgacgacgac aaacggatac tccacgcgcc agtcggttgc
     4801 atcggtaacg tgcgttaccc gcgccaatcc gtcaaacata atattgtgcg gcttggtgat
     4861 tttgctcaaa cgctccaaaa tctgccgccg cttttggctg gtttcatcaa aactgctatc
     4921 aaaacgctcg actgcttttt ctgctgcggc aaccaagaca tccacttgcc gattatcgtc
     4981 gcggcgtttg cggatttcgt tcaggcggtg gcgcatttcg cgtatcaacg cggcgcggcg
     5041 tttcggatgc tccagcaaat catcgaccag atacggattg cgcacgacca cccaaatatc
     5101 gcccaacact tcaaacagca tccgcgccga acgtccagtt ttacgctgtc cgcgcaagtc
     5161 ttgcagaatc tgccacgcct cgtcgcccaa taatcggatg acgatttcgc ggtcggtgta
     5221 ggaagtatag ttgtagggga tttcccgaat acgctgaggg gcggtagtcg tggtcatggt
     5281 gtgtccgtgt ttggcagttt ttatttgaat gccgtctgaa aagtcggttt caggcgagtt
     5341 tgaaaaggcg ataatgtagt tcaatttttg atacttttca atgccgaaac aggaaatacc
     5401 cttatagaaa caaaataacg ctttgagaaa ttgttgacaa tattatcaaa tataaatttt
     5461 aggtggtgtt tccggcgtat gaccgtatca aaacggttga gtttttcaga aaacccgtta
     5521 taatgccgtt tctctgaggc gggtctcccc gcatggcaaa tcggaacacc gggtcagggg
     5581 cggaagccag cagcccactc cgatgcgcca gtgccggggg tttggcccgc cgccctattt
     5641 gaaacgccgg agcttcgatg ttccggcgtt cccgtatcgc accgatgccg tctgaaagcc
     5701 gttcagacgg catttccttt actttgccat aacgcataac tacaatggga aaacaatttc
     5761 aatgtgcata tgcacctgcc gacaatttta aggaaactgc catgagcgca tcacaattac
     5821 tgtcaagact gacccaaacc gtaggcgaaa aatacatcat caccgacccc gcgaaaaccg
     5881 aacaataccg ccaaggctac cgcttcggcg agggtaaggc gttggcggtg gttcgccccg
     5941 gaagcattct ggaaatgtgg aaaattctgc aggcctgcgt cgaagcggac gtgattgtga
     6001 ttacgcaggc ggcgaatacc ggtttgacgg gcggctcgac ccccgacggc aacgattacg
     6061 accgcgacat cgtgattgtg aacaccatgc ggatgaacat catccaaacc atcaacaaca
     6121 acgaacaagt cgtctgcctg cccggttcga ccctgaacca gcttgaattg ctgctgaaac
     6181 ctttggggcg cgaaccgcat tcggtcatcg gctcatcctg tatcggtgcg tccgtcttgg
     6241 gcggcgtgtg caacaactcc ggcggcgcgt tggtacagcg cggcccggcc tacaccgaaa
     6301 tggcgttgtt cgcccaaatc aacgaagaag gcaggttgga actggtcaac cacttgggca
     6361 tagacttggg cgacacgccc gaagaaatcc tgaccaacct gcaaggtcat cattatcaaa
     6421 acaaagacat caaacaagac gcgggcaaag ggcacgacca cgcctattgc gaacacgtcc
     6481 gccaagtgga cgaaccgacc gccgcgcgtt tcaatgccga ccccgcccgc cattacgaag
     6541 cctcgggctg cgcgggcaaa ctgatggttt ttgccgtccg tttggacacc ttcccgcaag
     6601 aaaaacaaac cgccgtgttc tacatcggca cgaacgacat caacgagctg accgacatcc
     6661 gccgcgccgc cttgggcgaa tttgaaagcc tgcctgtttc cggcgaatac atccaccgcc
     6721 acgctttcga cattgccgac gtgtacggca aagacacgtt ttacgtcatc aaaaaattcg
     6781 gtacgcacca actgccgaaa ttatttgact tgaaggcgcg cgtggacagg ttcggcaaaa
     6841 aagccagctt cctgcccaaa catttttccg acaaggccat gcagttcgtc agcaaatacc
     6901 tgcccgacca cctgcccaaa tccatgcgcg attaccgcga caaatacgaa caccacctga
     6961 ttctgaaaat gggcgggaaa ggcgtggatg aggcgcgcgc gttcttaaaa gaatattttg
     7021 cacaccacag cggcgcgttt ttcgagtgca acgccgaaga aacccaagcc gcgatgctgc
     7081 accgtttcgc cgtcgcctcc gccgccatcc gctaccgcgc cgtgcatgat gacgaagtgg
     7141 aagacttggt cgcgcttgac atcgccctgc gccgcgacga ccgcgactgg tttgaaaaac
     7201 tgccgcccga aatcgacaat aaaatcatcc acaaactcta ctacggacat ttcatgtgcc
     7261 acgttttcca tcaggattac atcatcaaaa aaggcaacga ctgcatggcg ttggaacacg
     7321 aaatgctgca tctcttagac caacgcggcg cgcaatatcc cgccgaacac aacgtcggtc
     7381 atttgtatga agccaagccc gcgctcaaac agttttaccg caaactcgac ccgaccaaca
     7441 gcttcaaccc gggtatcggc aaaaccagca aaaagaaaaa ctgggcggaa taagcgcgtc
     7501 cgctttgaag gcaggcaatg ccgtctgaag gcaaaacgcc gttcagacgg cattgtcggg
     7561 tttgaagttc cccgtcgggt aatcgctttc aaaccgtatg ccgacacacg ggcgcgcccc
     7621 tcccaaatat agtggattaa atttaaatca ggacaaggca acgaagccgc agacagtaca
     7681 gatagtacgg caaggcgagg taacgccgta ctggtttaaa tttaatccac tataaagtct
     7741 gatcctacct tcccaaaggg cagggtttca accgaaaagg aaacacgatg aaaccataca
     7801 aaatctacac ccatcccgcc ctgccgcccc aagccgtcaa acaaggctgg tcatggccgg
     7861 ggctcttgtt cggcacgctg tgggcgtgtt tcaaaagaat gtggggtttg gggctggggc
     7921 tgacgggcgc gatattcgtg ctggctgtgt ttgcccaact ggtttacggc gacacgcccg
     7981 ccacagactc ggcgtttaac gtattgggct tggcggtttc cgtctggttc ggcgcaaagg
     8041 gcaacagcct ttatgcacgc caccttttat cgcgcggcta taccgaattg cccgaaacgg
     8101 ttgaggcagc caatccccaa gccgcattgg cgcaatattt cgggcgcggg ggccgataag
     8161 cgcgttttct tttccatcat gccgtctgaa aaatgttcag acggcatttc ttataacctt
     8221 ttgtttgcag cacataacca atcagtcttt cctattccgt tccgaaatgc ataccatgcg
     8281 cccgtaccca atatttgcga acaaggaaag aaaatggcac gtttaaccgt acacaccctc
     8341 gaaaccgccc ccgaagccgc caaagcgcgc gtcgaggcgg tacttcaaaa caacggcttt
     8401 atccccaacc ttatcggcgt attatcaaac gcccccgaag ccttggcgtt ttaccaagaa
     8461 gtcggcaagc tcaacgccgc caacagcctg accgccggcg aagtcgaagt catccagatc
     8521 atcgccgccc gtaccaacca atgcggtttt tgcgtggcag ggcacaccaa actcgcaacc
     8581 ctgaaaaaac tcctgtccga acaatccgtc aaagccgcgc gcgctttggc agcaggcgaa
     8641 tttgacgatg ccaaactcgg cgcgctcgcc gcctttaccc aagccgtaat ggcgaaaaaa
     8701 ggcgcggtat ccgacgagga actcaaagca tttttcgatg cgggctacaa ccagcagcag
     8761 gcagtcgaag tcgtgatggg cgtagccttg gcaaccctgt gcaactacgt caacaacctc
     8821 ggacaaaccg aaatcaaccc cgaattgcag gcttacgcct gatacggcaa accgcccgaa
     8881 tatcgggcgg tttttcaaaa acaccccctc aaacaaaaac aagccgccaa gcggcaggga
     8941 agcagccgcg catcaggcgc gcccccgatt cccccgcaaa ccttaaggaa caaagatgaa
     9001 cgcccaaacc ctgattgcca acgttgccga attcgtcaaa accaagctca aacccatagt
     9061 ggacgacatc gaccgcaaag gatactaccc cgaagcattt atgcgcgaac tcggcgcaat
     9121 cggtggattc ggcgcagtcg gtacagaagc cgaaggcggc aacggcttgg gtttggcaac
     9181 gcaaatcgcc gtattgcgcg aaatcggcaa agaatgcggc gcaacctctt tcagtgcgtg
     9241 gtgtcaggcg gcttgcgcgt ggtatctgca ccaaacgccc aaccgggccg tcaaagacaa
     9301 ataccttgcc gacatcctgc aaggcaaagt attggcgggt acgggcatgt ccaataccgt
     9361 caaacacctt gccgacatcg aaaaacacaa cctccaagcc gagcgcgtgg agggcggcta
     9421 cacagtcaac ggcgcgctgc cgtgggtgtc caacatcggc gaagaccaca tttgggcgaa
     9481 taccgcccaa atcggcggcg gctacgtcat gttcatcaca ggcggacaat gggaaggcgt
     9541 aagcctgcaa aactgccccg aattttgcgc cctcgaaggc acgcgcacct tcagcctgaa
     9601 cttcaaagac gtatttatcc ccgacgaaga catcatcgcc gcgcccgaac agtttgccga
     9661 ctacatccaa agcatcaaag caggctttat cctcctgcaa atcggcatcg gcgcgggcgt
     9721 gatagacggc agcctcggca tcatccgcct cgccaacgtc gtcaacgccg aagtcaaccg
     9781 ctatctcgac gacggctacg acagcctcaa aacaaggctg gacggcgcgt gggcagaaac
     9841 cgaacggctc gccggattgg cttggagcgg cacgcccgac aaccttacca ccctcaagct
     9901 gcgcgaagcc gccgccgtac tgacacttgc cgccgcccaa tccgccgccc tgcattccgg
     9961 cgcaaaaggc tacctgatgc ggagtcccgc ccaaaggcgc gtccgcgaag caatgtttgt
    10021 cgccatcgta actcccgcga tcaaacacct ccgcaaagaa atagcggcaa tcgaagccgc
    10081 aaaataaaca ggcggaaaaa accgaaaccc ccatcgcgtc attcccgcga aagcgggaat
    10141 ccagccccca acgtaacagg aatccatcgg aaaaaacaga aaccctcaaa ccgtcattcc
    10201 cgcaaaagcg ggaatccagc cccccctaac gcaacaggaa ttcatcgaaa aaaccgaaac
    10261 ctccaaaccg tcattcccac ggaagtggga atccagtaac cgaaaaacca caggaatcta
    10321 tcgaaaaaaa acccaacccc caaaaaaacc gggcggatac ctcaccatcc tcccaaaccc
    10381 tgatttatag cggatgaaca aaaatcaggg caagacggcg aaaccgcaga cagtacaaat
    10441 agtacgaaac cgattcactt ggtgcttgag caccttagag aatcgttctc tttgagctaa
    10501 ggcgaggcaa cgccgtactg gtttttgttc atccgctata caaaaaacaa ggaaacacaa
    10561 atggcgcaat atatgtgcgg cccctgcggc tggatttacg atgaagaaca cggcgaccca
    10621 gaacacggca tcccccccgg gacaaagttt gaagacattc ccgacgactg gaaatgcccc
    10681 gaatgcggcg tgggcaaaga agatttctac ctgttggatt tcgtgatata gcggcagccg
    10741 cacccttagg gaaaaccccg tcatccggcg ttccgcctgc ggcggcaggg aacgccgttt
    10801 tatagtggat taacaaaaat catgacaagg cgacgaagcc gcagacagta caaatagtac
    10861 ggaaccgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga
    10921 gccaacgatg tactggtttt tgttaatcca ctatatccgt ttgcgcccgc caagccgtgt
    10981 tgcacagatg cgacacggct tttttctttc tgttgtcaga atgtaaattt tcgtcaaaaa
    11041 acatcaaaaa tcaccccccc ccccgaattt gcgtaaaata aagtgtaatc cctttgatca
    11101 taagggaatg ttgacaatta gatcgattct cctaggctga ctttcgcaat aaatggggtt
    11161 gtcaataatt gtgtat
//
LOCUS       DAXIHS010000067        11159 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXIHS010000067 DAXIHS010000000
VERSION     DAXIHS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.067"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..183
                     /locus_tag="ACFOF2_001546"
     CDS             <1..183
                     /locus_tag="ACFOF2_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC7107833.1"
                     /translation="KKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQNHIN
                     GIENFWNQAKRVLRKL"
     gene            complement(420..1523)
                     /gene="dnaN"
                     /locus_tag="ACFOF2_001547"
     CDS             complement(420..1523)
                     /gene="dnaN"
                     /locus_tag="ACFOF2_001547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC7107834.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1758..3314)
                     /gene="dnaA"
                     /locus_tag="ACFOF2_001548"
     CDS             complement(1758..3314)
                     /gene="dnaA"
                     /locus_tag="ACFOF2_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC7107835.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3606..3740
                     /gene="rpmH"
                     /locus_tag="ACFOF2_001549"
     CDS             3606..3740
                     /gene="rpmH"
                     /locus_tag="ACFOF2_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC7107836.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3743..4108
                     /gene="rnpA"
                     /locus_tag="ACFOF2_001550"
     CDS             3743..4108
                     /gene="rnpA"
                     /locus_tag="ACFOF2_001550"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC7107837.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            4173..4394
                     /gene="yidD"
                     /locus_tag="ACFOF2_001551"
     CDS             4173..4394
                     /gene="yidD"
                     /locus_tag="ACFOF2_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC7107838.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4566..6203
                     /gene="yidC"
                     /locus_tag="ACFOF2_001552"
     CDS             4566..6203
                     /gene="yidC"
                     /locus_tag="ACFOF2_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC7107839.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(6284..7009)
                     /locus_tag="ACFOF2_001553"
     CDS             complement(6284..7009)
                     /locus_tag="ACFOF2_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC7107840.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVA
                     DWRRMKEMPNLKKRPTIFVMYAG"
     gene            complement(7056..7646)
                     /locus_tag="ACFOF2_001554"
     CDS             complement(7056..7646)
                     /locus_tag="ACFOF2_001554"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC7107841.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7794..8297
                     /locus_tag="ACFOF2_001555"
     CDS             7794..8297
                     /locus_tag="ACFOF2_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC7107842.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8331..8510
                     /gene="rpmF"
                     /locus_tag="ACFOF2_001556"
     CDS             8331..8510
                     /gene="rpmF"
                     /locus_tag="ACFOF2_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC7107843.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8685..9212
                     /locus_tag="ACFOF2_001557"
     CDS             8685..9212
                     /locus_tag="ACFOF2_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC7107844.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            9300..10355
                     /gene="plsX"
                     /locus_tag="ACFOF2_001558"
     CDS             9300..10355
                     /gene="plsX"
                     /locus_tag="ACFOF2_001558"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC7107845.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10491..10749
                     /locus_tag="ACFOF2_001559"
                     /pseudo
     CDS             10491..10749
                     /locus_tag="ACFOF2_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
     gene            10776..>11159
                     /locus_tag="ACFOF2_001560"
     CDS             10776..>11159
                     /locus_tag="ACFOF2_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107846.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTVYFEKGYYLPMIQTILEDGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHTSKNISTIQNIISEFM"
BASE COUNT         2900 a         3282 c         2597 g         2380 t
ORIGIN      
        1 aagaaaatca tgccggacag cattgtttat accgatagtc tgagcagctg cgacaagttg
       61 gacgtgagcg gttttatcca ttaccgcatc aaccattcca aggagtttgc agaccgtcag
      121 aaccacatta acggcattga gaatttttgg aatcaggcaa aacgcgtctt gcgaaaatta
      181 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacga
      241 aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
      301 caacgccgta ctggtttttg ttcatccact atacctttcc gacagccgaa caaaaccccg
      361 aatccgtctg cacggttcgg ggtatatctc caatacgggc atcgtgttcc ggaaaaccgt
      421 caaatccgca tcggcatcac aatatatttg aaattcggat tgttcggcac ggtaaacagc
      481 gtcgagcggt tggcatcgcc gaaggcaagc tgcatatcgt cggaatggat gttgcgcaac
      541 acgtccatca gatagccgat attgaaaccg acttcgagtt cgccgccctg ataggcgatt
      601 tcgatttctt cgcgcgcttc ttcctgctcg ttgttgctgc acacaacgct caacaggccg
      661 ggttgcaaaa acaatcgcgc accgcggaat ttttcattgg caagaatcga tgcacgttcc
      721 aacgcgccca acaattctgc cctcgacaac acgaaaatct tgtcgttgtc caaaggaatc
      781 acgcggttga aatcggggaa tttgccgtcg atgaccttgc tgacgatggt cgtgccgttg
      841 cattggaaac gcacctgttt gtccagcagc tcgatttgaa tcggatcgtc ggggttgttc
      901 aacagtttga acagttccag caccgttttg cgcggcaaaa tcacttcggc gcgcggcaaa
      961 tccgcatcaa tcgcgcaggc tgcataggca aggcggtgtc cgtcggtcgc cacaaggcgc
     1021 aactggctgc cctcaacctg catcagcaga ccgttgagat aatagcggat gtcctgcacc
     1081 gccatgctgt actgcacttg cgacagcatg gttttgaaac gctcctgctc cagcgagaaa
     1141 gtcgcgctga tgtcctcgcc gacattcatc atcggaaaat cggcggcagg cagggtctgc
     1201 agggcaaaac gcgatttgcc cgccttcagc gtcagacggc tgtcgtccca atccagcgac
     1261 accagcgcac cggcaggcag cgcgcgcaaa atgtcctgaa atttcttggc attggtggtg
     1321 atgcggaagt cgcccgcgcc gccctcggga cccgcagtgt cgatttggat ttccaaatcg
     1381 gttgccaaga gtttggtctg accgcctttt ccctcaatca ggacgttgga caggatgggc
     1441 agggtgtggc ggcgttcgac aatgccggta acggcttgca acggcttgag caggctgtcg
     1501 cgctcggctt gtaaaatcaa catgttcgct cctttaaatc ggtttgtata gtggattaaa
     1561 tttaaatcag gacaaggcga cgaagccgca gacggtacaa atagtacgga accgattcac
     1621 ttggtgcttc agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac
     1681 tggtttaaag ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg
     1741 tctgaaaacg ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc
     1801 aattccggat cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta
     1861 tggtcgcgcc caccaaacga atcgccgata gacggcaggc tcaaagtagt cagttctttg
     1921 gtcaggctca tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc
     1981 acatcgctga ttttgatgcg gtaatatttc gccaccgcat cgatgatgat gtcggcggtg
     2041 atgactttgt gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg
     2101 atgacgggac ggttcataaa gcggctgctc gctccgacac gattaaacgc gccttcaagc
     2161 tcgcgcacgt tggaacggat cagattggca atgaacagcg cggcttcgtc ttcgatactg
     2221 atgcccgccg cttccgcctt tttctgcaaa atggcgatgc gcatttccaa ttcgggcggc
     2281 tcgagttcca aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc catgccttcg
     2341 attttcgcag gcaacacatc gcaagtgagg atgagctgtt ttttctcgtt gtggaaatgg
     2401 ttgtacagat agaaaaactc ttccatcgta cggtctttgc ctttgatgaa ctggatgtcg
     2461 tcgataatca gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta agtgttgttg
     2521 cgaaccgcct tcataaagct gcggatatag tcatccgaat gcatatagcg cactttggca
     2581 tcgggacggt ttttcagcag ctcgttgccg accgcctgca caaggtgggt tttgcccaaa
     2641 cccgtactgc catagaggaa gaacgggttg taactctgcc ccgggctttc cgcaatcgcc
     2701 tgcgccgcag ccgccgcaag gcggttgccc ttaccttcta ccaacgtatc aaacgtgtaa
     2761 tccggagaca ggttggtctg ctcgtaacgc gcctcttccg catcgcgctg cacgtccgtc
     2821 cgtgctttgg caactgccac cgattccggc cgggaagcag acccggcagc ctgacgcggc
     2881 tcgtgcggca ggtttttcat acgttccgcc aaaatatccg ccgccgtttt cgacgcagcg
     2941 ggtttgacag gctcttcaga cggcagctcg tccaacagaa cctcctgcac gggcattccc
     3001 tccgacaccg catgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc
     3061 tcataacgca cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct
     3121 tccctcaccg cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg
     3181 ttcttgccgt acaccaccca tacgccaccc tcctcaccaa cggtaagggg cgcaatccat
     3241 tgcgcaaact gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac
     3301 tctgctaatg tcatggataa gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata
     3361 taatattttc aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc
     3421 cgattatacc gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa
     3481 ggcggcggaa atccgcaggc aatcgggcaa tcttcctgca aagtttctat attgattgac
     3541 aaaagcggca aattggagtg taattcacgg tttaattatc tacccattct attttaggaa
     3601 acatcatgaa acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc
     3661 tggtgcgctc caaaacgcgc ggcggccgcg cagtattggc cgcacgccgt gccaaaggcc
     3721 gcaaacgcct ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa
     3781 aacggatgat ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca
     3841 agtttcgcgc tcaaacggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa
     3901 aaaaaccgcc aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt
     3961 tagattgaac aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt
     4021 cgacagggct accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc
     4081 cgcaaccgga tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac
     4141 ggcaacgggt ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc
     4201 tgggactgat acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt
     4261 atacgccgac ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag
     4321 gcggccggct cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg
     4381 accccgttcc ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg
     4441 gtttctatat aatgccgtct gaagcttcgg gcaggcggca cgaccgccgg gtatgaagcc
     4501 cgcccttatt ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa
     4561 ctcttatgga ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg
     4621 gctgggaaaa gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac
     4681 aacaggccgt aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa
     4741 cgaccgacac ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc
     4801 tgctcaaata caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca
     4861 aagaatacac ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa
     4921 aaggcatcgg ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag
     4981 tccgcctgag cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca
     5041 aaggcagcta tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca
     5101 acctgagcgc ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact
     5161 ttacccactc ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca
     5221 gcttctccga cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca
     5281 aaaccccgac cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc
     5341 aacccaaagg cggacaaagc gtttgcgccg ctggcgactg ccgtatcgac atcaaacgcc
     5401 gcaacgacaa gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg
     5461 cgaaagccga agcctccatc aacctctacg ccggcccaca gaccacatcc gttatcgcaa
     5521 acatcgccga caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcttccc
     5581 cgctcttctg gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta
     5641 tcgttttaac catcatcgtc aaagccgtac tgtatccatt gaccaacgcg tcctaccgtt
     5701 cgatggcgaa aatgcgtgcc gccgcgccca aactgcaggc aatcaaagag aaatacggcg
     5761 atgaccgcat ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc
     5821 cgctgggcgg ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg
     5881 cattgttcgc ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca
     5941 gccgcgccga cccctactac atcctgccca tcattatggc ggcaacgatg gtcgcccaaa
     6001 cttatctgaa cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt
     6061 tggttttctc cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca
     6121 acaacctcct gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca
     6181 cccaaggcga agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc
     6241 agacggcatt tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca
     6301 caaaaatcgt cgggcgtttt ttcagattgg gcatttcctt catcctgcgc caatccgcaa
     6361 ccgttttact gaccaccgac tgagtcggca acgtcaaatc cgtagccgtg cacaagcgcg
     6421 tttcaggatg caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt
     6481 cgataaacag ctgcgtttca ttctgctggc gcgaacgttg ttccaaagcc ttcaaactct
     6541 gaatccgctc atttttttca gacggcaaat aacccttaaa cgcaaagttc tgcccattcg
     6601 cacccgaagc catcagcgcc agcaacaagc tggacggacc gaccaaaggc cgtacttcaa
     6661 aaccgtgttt atgcgccaat gccaccaaat tcgcacccgg atcagccaca gccgggcaac
     6721 ccgcctcgct gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca
     6781 aagtcttcaa atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg
     6841 tcacgcccaa atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatccg
     6901 tcagcccgac aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca
     6961 aaggcgtagg aatcaaatac aaaacaggag acaccattcc ctcactcatc ggttaaaaat
     7021 gccgtctgaa cccttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt
     7081 ttcaaaaaat caaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc
     7141 tcaatccttt cgatcaacaa tgcacccaaa gcctcgctct tcaacgcaca cgagcaataa
     7201 accgcatcag gctcgcgctc caaatagcgg aggatgtgta gctcgtccaa cttcctcatc
     7261 acgaccaccg tcttatcgat atgccgctgc atcctgcccg taaccgtatt tagcagaacg
     7321 accgcgctgt aaaactcaat ctccctgccg ctcaaatgca tcaacatctt gtgcgcatta
     7381 gcaaggttca tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg
     7441 acaatcaacg cctctgggaa acgcccggcc aacgaccgcg ccttaccctc ggcaaggcgc
     7501 aatgccgtct gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc
     7561 gcctgaaagg caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc
     7621 aaaatcaaag gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag
     7681 gggcagtaaa atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca
     7741 tttttcttaa aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag
     7801 accctaattt gattgactcg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt
     7861 ttctgctgga agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga
     7921 ccaaaatatc gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc
     7981 tgaacgtcaa agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca
     8041 tgcttgatga aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca
     8101 tgcttgccga cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat
     8161 tggtagaaga ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg
     8221 acaatgggac actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag
     8281 gtttgaaaag caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc
     8341 aacaaaacaa aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg
     8401 cgcccgcact gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatttccc
     8461 ccaacggtat gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg
     8521 actgactgaa aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca
     8581 ccatccgtcc aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataataa
     8641 cccgtgaaaa tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct
     8701 ggtacaccta cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg
     8761 ccgacatcaa atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta
     8821 tgctgcgctg ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg
     8881 acaacgaagg acaggttaca gaagaagtca aaaaagtcca atggctcgac cccgttcccg
     8941 aagccctgcg gggcaaaaac gtactcgtcg tcgatgaagt ggacgacagc cgcgtaacca
     9001 tggaattttg cctgacagag ctgctcaaag aagacttcgg tacgatcggc gtcgccgtac
     9061 tgcacgaaaa aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg
     9121 gcatcaccgt agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac
     9181 acaaccgcct tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc
     9241 gatgccgtct gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaacccta
     9301 tgattacact tgccgtagat gccatgggcg gcgaccaagg acttgccgtt accgtacccg
     9361 gcgcaaccgc attcctccaa gcacaccccg atgtccgcct gattatgacc ggcgacgaaa
     9421 cgcaactgcg ccaagccctg accgcggcag gcgcaccgat ggaacgcatc gacatctgcc
     9481 ataccaccca agttgtcggc atggatgaat cccctcagtc cgccctaaag aacaaaaaag
     9541 actcctccat gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa gccgccgtat
     9601 ccgcaggcaa cacgggcgca ctcatggcaa ccgcacgttt cgtcctcaaa accattcccg
     9661 gcatcgaacg ccccgccatc gccaaattcc ttccttccga caccgaccac gttaccctcg
     9721 ccctcgacct cggcgcaaac gtcgactgca cgcccgaaca gctcgcccaa tttgccgtta
     9781 tcggcagcga actcgtccac gcactccatc ctcaaaaagg acagccgcgt gtcgggctgg
     9841 tcaacgtcgg cacggaagac atcaaaggta cggacaccgt caaacaaacc tacaaactgc
     9901 tgcaaaacag caaactcaac tttatcggca acatcgaaag caacggcatc ctgtacggcg
     9961 aagcagatgt cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa accatcgaag
    10021 gcgcggtcaa attcatgagc ggagccatcc gccgcgaatt ccaaagcaac ctgttcaaca
    10081 aacttgccgc cgttgccgcc ctacccgccc tcaaagggct gaaaaacaaa ctcgatccgc
    10141 gcaaattcaa cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa agccacggcg
    10201 gcacagacga aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa gccaagtccg
    10261 ccggcctttc caaaatcgaa caaggcgttg ccgaacaact cgccgcactc gaaactgcca
    10321 aagccgtcca aaacgaaaat gccgacggtc tgtaacacac acgatgccgt ctgaacgccc
    10381 ccgccccttt cagacggcat ccgcccgccc cgaacctgcg ggcgcggacg gcgatgcacc
    10441 tgtccggcac ttcccaaata cttccctgta aaataaggag tatttgaaaa atgaagacat
    10501 tagaaaaacg gatgaaagct ctagacaaac agataatgaa gttcggaaaa tcccttgaag
    10561 gcaggcttga ggcttgatgc ccgtctgatt gaatccgcat tggattatat tcattattcg
    10621 gaacgttttt tggcttttga aatcctgtgt acttatatcg aagatttcga tgtccggctg
    10681 acggaacaag aatcccggga aatttctttt atcaacaagg aatttgagat agaaagcacg
    10741 tccgattaac caataaagcc aatgggttga taaacatgaa aacatcaacg gttgtttttg
    10801 gcggattttt tattacagat aatggagaac gaatccaaat ccccgttttg gaaaatcctg
    10861 gcattaaaga aatcaatcac tttttttcca tatcaaattt tgagaaaaaa gccggcgtcc
    10921 ttgttttcag aatcatcccc gagccggaat ttggcgatac cgaattaact gtctattttg
    10981 aaaaaggata ttatttgcca atgattcaaa caattttaga agacggggat attgaagtca
    11041 aaaaccttaa aacggaaaat tacagtggga aggcaaggga aattttggga gattcctacc
    11101 ctattgagca tacctctaaa aatatttcta caattcaaaa tataatttct gaatttatg
//
LOCUS       DAXIHS010000068        10973 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXIHS010000068 DAXIHS010000000
VERSION     DAXIHS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.068"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..75
                     /locus_tag="ACFOF2_001561"
     CDS             <1..75
                     /locus_tag="ACFOF2_001561"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC7107847.1"
                     /translation="NDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(392..754)
                     /gene="ssrA"
                     /locus_tag="ACFOF2_001562"
     tmRNA           complement(392..754)
                     /gene="ssrA"
                     /locus_tag="ACFOF2_001562"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(817..1422)
                     /locus_tag="ACFOF2_001563"
     CDS             complement(817..1422)
                     /locus_tag="ACFOF2_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC7107848.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(1813..1998)
                     /locus_tag="ACFOF2_001564"
     CDS             complement(1813..1998)
                     /locus_tag="ACFOF2_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107849.1"
                     /translation="MPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKMPSE
                     AVGATILIWGALHQRGA"
     gene            complement(2004..3059)
                     /locus_tag="ACFOF2_001565"
     CDS             complement(2004..3059)
                     /locus_tag="ACFOF2_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7107850.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGSSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(3165..3647)
                     /locus_tag="ACFOF2_001566"
     CDS             complement(3165..3647)
                     /locus_tag="ACFOF2_001566"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC7107851.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(3694..4830)
                     /locus_tag="ACFOF2_001567"
     CDS             complement(3694..4830)
                     /locus_tag="ACFOF2_001567"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC7107852.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(4827..5372)
                     /locus_tag="ACFOF2_001568"
     CDS             complement(4827..5372)
                     /locus_tag="ACFOF2_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC7107853.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(5369..6844)
                     /gene="trpE"
                     /locus_tag="ACFOF2_001569"
     CDS             complement(5369..6844)
                     /gene="trpE"
                     /locus_tag="ACFOF2_001569"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC7107854.1"
                     /translation="MISKQEYQAQAAQDYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPIGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDNVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWSFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            complement(6956..7321)
                     /locus_tag="ACFOF2_001570"
     CDS             complement(6956..7321)
                     /locus_tag="ACFOF2_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107855.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     ATAGYLFKWKQYQQRKTKP"
     gene            complement(7342..9252)
                     /locus_tag="ACFOF2_001571"
     CDS             complement(7342..9252)
                     /locus_tag="ACFOF2_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC7107856.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(9271..9915)
                     /locus_tag="ACFOF2_001572"
     CDS             complement(9271..9915)
                     /locus_tag="ACFOF2_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC7107857.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(10079..10897)
                     /gene="opc"
                     /locus_tag="ACFOF2_001573"
                     /gene_synonym="opcA"
     CDS             complement(10079..10897)
                     /gene="opc"
                     /locus_tag="ACFOF2_001573"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC7107858.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2370 a         2385 c         3103 g         3115 t
ORIGIN      
        1 aacgatagaa tcaagttgaa attaaaaggg ctgagccttg ttcagtacag aattcagtcc
       61 ctgaaagccg cttgattaaa ctgtccgact ttttggggtc agttcggctt cggcattttt
      121 ttatccgttt gggggtaact tgtttggaaa gctgcaagct tataaataaa ggattacatt
      181 taagttttgg gtaacctttt taaaaaaatg cgtgatgact tttgcatttt taaggcgttt
      241 tttggggtaa ttcgtgaaaa gttaccccaa aagttacccc ataaatggcg aaaactcaag
      301 catacgccag catcctgcaa cacaaaaaag ccttgaaact gttgaagttc aaggcttttt
      361 tgtgttgcag gatgctgctg aaaatagggt atggtggagg cggggggaat cgaacccccg
      421 tccgaaagtc ctctacaaag cgttctacat acttagttgt gtctatttga aaatcttatt
      481 tccatcatgc cgaccaacag gccttttgga aaccagttac cttaagtctt atttcctgcc
      541 aagtaacccg gtaggaaacc agtcaatgta agatgacgtt gcggtggctt tcgccacaca
      601 gcccattgac cgactgctgc aacggctagc cttaagcggc taaagcgtaa gtttcgtcgt
      661 ttgcgactat ttgaattcag tgttttacgg gaatctgaga ccccggtatg cccgcatctg
      721 cttcgcaacc ctcgtcgaaa ccaaggtcgc ccccagaaat ggtttgcaaa ttatacggat
      781 attgtgcggt gctgccaagt ctgtcggaga aatttgtcag tcttgctgcc ttaatttgcg
      841 tttgagcagg atgcggacgc agccgtcgtt gccttcccgg ggttcggcgt aggcgagtac
      901 gtcggggtgt tgcatcagcc agtttcgggt catatttttc agaacgggtt tgtagccttt
      961 ggaacctaat ccgctgccgt ggatgatttc gccgcatacg ccgcgttttt gggtgaatgc
     1021 gatgaattcg ttgaggactt tttgggcttc ttcctgtgtg tagccgtgca ggtcgacatc
     1081 ggtaacgacg ggatagtatc cgtttttcag gcgttggatg tcgttttttc cctgtccgtt
     1141 tttgctgaag ctggcgggcg ggtcgttgta tgtgctgccg atgtagaagt agttttcttc
     1201 gtcggcgcgg ttgtctttgg gacggacttt gatgggggtt ttgtcgggcg gcgcataata
     1261 ttgctgccgg ttttttaatg gggagacttg tccgactgct tgtgaaaaat cgaaatcctg
     1321 ttcttgtttt tgcttgtttt tttcggctgc ctgtttttct gccgcttctt tttgggcttg
     1381 tttgcccagt tgtttgagga tgttttggaa gtcggtattc atattttttc ctgttatttg
     1441 tccgatggct gtttcgggcg gggttttaat ttgccggaat gtttaccaat cgggggagga
     1501 tgattttgtt gcctgcgtat gttttttgaa agtgtgattg tatatcaaaa agaaatgcgg
     1561 caaccgtcgg cagtgttgat tgccggaaat gcggaccggt cgaaccgata tgcccgaacg
     1621 cctgataaag ttttaaaaac ctgccttgcg aagcaggctg acgtgttttg ccaatcttga
     1681 attgccggaa acgcgaaaca cggaaatctg atgttttata aatgttccga tacgaactgc
     1741 aaaatattgg ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaagggat
     1801 ggttaaggaa agttatgcgc ccctttgatg aagcgcgccc cagataagga tggttgcgcc
     1861 gacggcttca gacggcattt tggcggcggt gttgggtttt gtatccggtt tgccgttgtg
     1921 tcttgtgatg atgattttgg gcgcggtttt tctgttttga tgtgtggaat gccgtctgaa
     1981 aggcggttca gacggcatag cggtcatttt tgtgcggtca ggcggtcgaa tatgccgccg
     2041 tcggcgaagt aggttttcat gatgttgtcc catccgccga atttttcttc gggagagaat
     2101 gtgtctaagt ctgggaagtc ggctttgtgt cttgccaata cttcggggtt gcgggggcgc
     2161 aggtagagtg aggcggcgag ttcttgcgcc ggttcgctcc aaaggtattc gagataggcg
     2221 cgggcggttt tttgcgtgcc ttttttcgcg acgatgctgt tgacgacggc gacggggctt
     2281 tcggcggaaa tggtgtagct cggatagacg atttcaaact gtccttgggt cagttttttg
     2341 ctgacgtagt tggcttcgtt ttcaaaagta atgagtacgt cgccgatgtt gcgttgtgtg
     2401 aaggtggtgg tggcggcgcg tccgccgttt tcaaaaacgg gggtgttttt gaggatggat
     2461 gcgacgagtt tttgggcttc ctgttcgttg ccgttggtgg ctttcagacc gtaaccgtat
     2521 gcgccgagga aggcgtagcg tccgttgccc gaggttttgg gattggcgat gacgatgtta
     2581 acgccgtctt tggcaaggtc gttccaatcg cggatctgtt tggggttgtt ttttcggaca
     2641 aggaaaacca tagtgctggt gtagggcgcg gcgtggtcgg ggagggcttg ttgccagcct
     2701 ttttctacca gtcctttttt ttcgagcagg tcgatgtcgg aggattggtt catggttacg
     2761 acatcggctt gaaggccgtt ggctacggat aatgcctgtt tgctggagct gccgtgggac
     2821 tgttggatgc tgacggatgt gccggggtgt tcggattggt atgttttgat aaataagggg
     2881 ttgtattctt tgtaaaaatc ccgtgccaca tcgtatgagg cgttgagcag ggtaatgttt
     2941 tttccgtcgg attcggtatt ggccggggca ttttgtccgg acggatggtt tgaatcggct
     3001 gcggggctgc aggcggtgag cagggctgcg gtatagagtg ccggtgcgta ggttttcata
     3061 tgcttgtcct gtcggttggt agatggggca actttatacg gctgtctgcg cttgtggaaa
     3121 taatgtttga tttgaagatt atcagttttg gttataagga cggatcagag gtgtttccgc
     3181 atcagttcgc atttgatttt gatgctgggg tcaagctgca atactgccga accgagcgat
     3241 tcgtagcggt tgagaaggta aaacgggact gagttgagcg atgcgtagag tttcagaaag
     3301 ctcaaaccgg atttgtgggc gatggtttcc gcctgatgaa gtagggcagt gcccagtccg
     3361 aggttgtgga acagggggtg gacgtaaagt gcatcgagtt gtgcttcttg gcagtcgatt
     3421 tggaaaaatc cctgtatgtt gcctttgtat tcggcaaccc aaagtgcttt gtcgggatcg
     3481 gaaatggtcg gcaggtagct ttctgtgttt aacaagcctt cccatacttt tagggcgtgt
     3541 tcgttgtagc tgaggatgca ggtgtattgg acggagtgca ggtggacttt gaagatgtct
     3601 ttgcagtctt gcacggtggc ggggcgtaac agtgtcagca ggttcatggc agtatgtcgg
     3661 cggcttcaga cggcatctgt gccgttggtc ggattatagg gactgatgca gtttttttgc
     3721 ttcttgaaat gcggtgtccg aatcggtggt taaaacggta aagtgtccca ttttccgccc
     3781 tttgtgcgcg gtttttttgc cgtaaaggtg caggtgcgcg tccggccggc tttgcagggg
     3841 aaaccaatcc ggttcgccgc cgtcttcctg ccaaacgtcg cccaaaatat tcgccataca
     3901 gcaaggcgat aataatttgg tgtcggcagg cggcaggttg cacataatgc gtacctgttg
     3961 ctggaattgg tctgccgcgc aggcatcgac ggtatggtgg ccggagttgt gcggacgcgg
     4021 cgcgatttcg ttgacgacca attcatgtgt gtcaccgaca acaaacattt ctaccgccaa
     4081 tacgccgaca taatccaatt cgtccgccaa gcgttgcgcc atctgccgcg cctgttgctg
     4141 cacgtcggca ctcagtcgcg cggggacgat ggaataagcc aagatgccgt tttcgtggat
     4201 gttttcggca ggatcgaaag tttgcacgtt gtcattgttc agacggcata cgataacgga
     4261 aatttcgccg cgcaagtcca ccattttttc caaaacgcaa tccacgccgc ggtgttcggc
     4321 aaacgcggct ttgagttcat ccaccgtttt tacgcggatt tggcctttgc cgtcatagcc
     4381 caatgtagcg gttttcagga tgccgggcag aaattgtatg ctttcttcag tgatgtcttc
     4441 ggctttgcaa attgcttgat acggcgcggt ttgcaggcct gctttgcgta tccatgactt
     4501 ttcctgaatg cggttttgcg cgatggcaac gcagtcgccg ctgggggaaa cattggtatg
     4561 tttggcgaga aaacgcatcg catcggcatt gacgttttcg aactcggtcg taacagccgc
     4621 acattttgcc aattcttcca aagcggtttg gttgtcaaac ggcgcacaca aatggcggtc
     4681 ggcaaattcc gctgccggcg cattcgggtt gggatcgagt acggttactt tgtagcccat
     4741 ggttttggca gcaacagtaa acattctgcc taattgtccg ccgccaagaa tgccgagcat
     4801 ggcgggcgga gaaagagata tgtttttcat gctgactctt caaattgtac aagttgatag
     4861 ctataactaa ttcttgacgg atgtcttgta tcgctggaat taccagtttc agaaatacag
     4921 aatacttttt ccataaattt ttctgctttt agaaattcca gtattctgtt tttttcatcc
     4981 ttataagcac cgcggtctgt accccatgca agaataatca tatcagcatc ttccaaacat
     5041 cctttaaatt tggaaaaatc ggtttgggtg tttgccctaa ttcctgtttg ctgtgtagag
     5101 taactggaaa aaatatttaa cattttgaag ttggtaaaac cgtacatatc caagaaacgt
     5161 gcaagttggg taagggtttt gtcgcttctt tcatcatttg ctttacttgg attaattcct
     5221 atagcaacag ctgagaaatt tttaggattt tctgtgttgc cgctccatct aaccgttagg
     5281 atttctcgac ttttttcatt atctgtatag agaccgtctt tggtagtcgg tctgagtgtt
     5341 tggcgaagct cataaagttt ttcataagtc atttatccaa cccttcctgc accatctgcg
     5401 cggcgtgtat gacggctcgg gctttgtttt gtgtttcctg ccattcggag gccggatcgg
     5461 agtcggcaac cacgcccgcg ccgctttgga cgtatagcgt gttgtttttt actacggcgg
     5521 tgcggatggc gattgccaaa tccatgtcgt tgttgaaact ccatacgccg acggcgccgc
     5581 cgtagatgcc gcgtttgctg ggttcgactt cttcgatgat ttccatggcg cggactttgg
     5641 gtgcgccgga aagtgtgccg gcggggaacg tggcggcgag gatgtccatg ttggtcatgc
     5701 cgtctttcag acggccttcg acgttggaga cgatgtgcat tacatgggag tatttttcaa
     5761 tcaccatttt gtcggtgact ttgacttcgc cggttttact gatgcggccg acgtcgttgc
     5821 gccctaaatc gataagcatg acgtgttcgg cgatttcttt ggcgtcgctt aataagtctt
     5881 gctcgttggc aaggtcttcg gcgggggttt tgccgcgcag gcgcgtgccg gctatggggc
     5941 ggacgataac gttgtcgcgt tcgcggcgga caaggatttc gggtgaggaa ccgacgatgt
     6001 ggaaatcgcc gaaatcgtag taaaagaggt aaggcgaagg gttgagcgtg cgcagggcgc
     6061 ggtagagggc gagcgggctg tcggtgaatt ccatgctcat gcgctggctg gggacgacct
     6121 gcatacagtc gcctgcgaag atgtagtctt tgattttatc gacgcaggct ttgaacggct
     6181 cttcgccgaa ctcgctgacg gcttcggtgt gtttgctgcc gagcgagagt gggatggcgc
     6241 agctttggcg cagctgggtg cggatgtctt cgaggcgttc gcgggcgcgt tcgtagccgt
     6301 cgggctgcga cggatcggcg taaacgacga gatggatttt gccgctcaaa ttgtcgatca
     6361 ccgccaattc ttgcgacagc atcagcaaga tgtcgggcgt gccgatcggg tcggctttgg
     6421 tggtgttttt caggcggtgg gcgaagtgtt caaaattgta gatggtttcg taaccgaagt
     6481 agccgaccag tccgccggta aagcgcggca ggcttgggat ttcgggcgtt ttgaagcggt
     6541 tgtggaaggc ttcgataaag ggcaggggat tgccgtcgtg ttgttcgacg atttcgccgt
     6601 tttgataaac atcgacgtgt ttgccgctgg ctttgagata gtggctgcaa ggcaggccga
     6661 taaaagaata gcggccgaaa cgttcgccac cgacaacgga ttcgagcagg taggtatagg
     6721 ggcggttggc gagtttgaga tagagggaaa gcggcgtatc caagtcggca agaagttctt
     6781 gcacgagcgg gatgcggttg tagtcttggg cggcttgggc ttggtattct tgtttgctga
     6841 tcatttctgc tttcccaaag ggcggttttg gacggcgcga caatgggcgc gagtatagca
     6901 ttttatcgga attgttgaca gtctgaccgg agatgccgtc tgaaaaaaga cgggcttacg
     6961 gctttgtttt gcgctgttgg tattgtttcc atttaaacag ataacctgcg gttgcgggaa
     7021 ttagggctaa gatggttttg aacgtccatg atgcatacca aggtaaagaa ccgggtgcga
     7081 ccgattgatt cagatacggc caccaacagg cagtcaggca gccgagcagc agcggccaga
     7141 tatgccggct gaggtttttt tgccaaagga ggaatgatgc ggcagaccaa agcagggtaa
     7201 gggcagtgat ttttaacacg gtataagcgt ccgctccaaa cagggcggcg agaaaatacg
     7261 taccggcaat ccagaggacg gcaagtatca ggacgatgat ttcttcaggt cgtttgaaca
     7321 tttttttctt cctgtttgat ttcagacggc attgccgttc tgtttggttt ccagcagctc
     7381 ccagcgttcc agcttttcca aaagcagcat ttcgatttct tcggcgcggc tttgcagtgc
     7441 gccggctttt tcgtaatctt tgaagatttc gggatcggaa agctgcgcgt tgatttctgc
     7501 ctgttcggct tccaaggcgg cgatttcgtc gggcagggca tcgagttctc gctgttcttt
     7561 gtaggacagt ttgaccgtgc ggttggcttt gggtttttct ttcaccggtt cggcatcggc
     7621 tgttttgggt gcggattccg tctgaatctt cgcttcgcgt gattttgcgt cgatgtagtc
     7681 ctgatagccg ccgatatatt cttttaacct tccatttcct tcaaaaacaa ggctttgggt
     7741 aatcacgtta tcgaggaaca tacggtcgtg cgagacaagg aataccgtgc cttgataatc
     7801 gcgcaacagg tcttcgagca gctcttgggt gtcgatgtcc aagtcattgg tcggttcgtc
     7861 caagaccaaa atattggcgg gacgggtaaa gagttttgcc agcaaaaggc ggttgcgttc
     7921 gccgccggag agtgaagaaa caggactttg cgcgcgggca ggggggaaca ggaaatcttc
     7981 caaatagctc atcacatgct ttttcttacc gccgacttca acgtaatcat tgccctgtcc
     8041 gagcgtgtaa aacacggtgt cgttttcatt caacgcgctg cggaactggt cgaaataggc
     8101 gacttcctgc ttgctgccga tacggattct gccgtaggtc ggctgcaatt cgcccaaaat
     8161 cagttttaaa aatgtggttt tgccgatgcc gtttggaccg attaagccga ttttgtcgcc
     8221 gcgctgcaag atggcggaga atttgtccat aatgactttg tcgccataag caaacgaggc
     8281 gtgttccagc tcggcgatga ttttgccgct tttctcgccg ctgtcgagct tgaaattgac
     8341 ctgcccttgt acgttgcggc gttcggcacg ctggcggcgc agttcttcca aacggcgcac
     8401 gcggccttcg ttgcgggtac ggcgcgcttc gatgcctttg cgtatccacg cttcttcctg
     8461 cgcgtggaat ttgtcgaaga ggcggttgtg ttccgcttcg actgccaact cttgcgcttt
     8521 tttctcgcta tatttggaga acgagccggg ataggaacgc aaaataccgc gatcgagttc
     8581 gacaatccgc gtggcgatat tgtccaaaaa acggcggtcg tgggtaatca caaccaagct
     8641 gccttcaaac gccttgagca ggttttccaa ccagataatc gcgtcaatat ccaaatggtt
     8701 ggtcggttcg tccagcagca atacgtcggg cttctgcacc caagcctgcg ccaaggcgac
     8761 acgctttttc tgaccgccgg aaaggttgcc gattttttcg ttttccggca aaccgagttc
     8821 gcccaaagtc tgcttgactg ccgcatccag cttccagcca tccttcgctt cgatttcaag
     8881 ttgtaattcg ttgagttctt tcaacaaagc ctcacccgaa ccgttttcca actcatgacc
     8941 gacgcggtgg taacggcgca ataaatcgcg aattttaccc aaaccttcgg caacggtatc
     9001 aaatacggtt gcgtccttat caaaaaagga ttcctgtggt acataaacga ttttgaggtt
     9061 gttttgaaca ataatctgcc cgtcgtcgag cttttgcaaa ccggcgagga tttttaaaaa
     9121 cgaagacttg cctgcgccgt tgcgtccgat taagccgact ttttcgccgc tgtcgagttg
     9181 aaaagaagtt ttgtcgagca aggcaacgtg accgatggca aaagaagcgt tttctacaga
     9241 taatatattc atgatgcaaa ttctcaacag ttaccgtttg gattttaccg caagtttggc
     9301 gcgggcaatt tcaaccgcac ccggcaggac ggaaacaata atgatgccgc ccatcaccaa
     9361 gcccagattg ttttttacga cggggaagtt ggcaaagaaa tagcccgcgt aagaaaacag
     9421 gataacccac aacaagccac cgatgatgtt gtagcggata aatttggcat agtgcatttt
     9481 ccccataccg gcgacgaagg gggcgaaggt gcggacgatg ggcataaaac gggcaatgat
     9541 gatggttttg ccgccgtgtt tttcgtaaaa acggtgggtt ttatcgagat attcacgtcg
     9601 gaagatttta gaatcggggt tggcgaacag cctgccgccg aaatatttgc cgacggtaaa
     9661 attgagcgcg tcgccgagta tggcggcaag gcttaataat gcaaccatca aatgaatatc
     9721 cataccgccc agcgcagcaa tcccgccggc ggcaaacagc agcgaatcgc cgggcagtaa
     9781 gggcgtaaca atcaggccgg tttcgcaaaa aacaatcaaa aacagaatcg cataaatcca
     9841 cacaccgtat tgcgccgaca gcgcgagcag gtgttggtcg atatggagga tgaagtcgat
     9901 ggcggaggca agcacggcgc gttcctaaaa aaaacaaacc gcgtatttta accgattgga
     9961 aaaatgccgt ctgaaatttt cagacggcat cggctattca aattcatttc acgtaaaaac
    10021 cgcaaaccaa aatagtttgc ggtttggcat ttaaagtgac aatgatgatt tcaaatcatc
    10081 agaattttat gccgacgcgc aagccgtatt cacgaatact ggttttcggg atggtgagcg
    10141 atacgtcgcc actcttggtt gttacactaa actcgccgga ttctttgtaa gtgcgttgtt
    10201 tgtagaacgg ccccgcctcg atgctggcgg attcgcccag ttttttacca atatttgcac
    10261 ccaatccaaa gccccagccg ttggtttttt gattgatgtc ttttttgaga ttggtaacgc
    10321 cggtgtttaa tttatagcgg gaattgaggt caaatttcac ttcagaccaa gggttgatat
    10381 accagccgtt acccagttgg gaaagcaaat ccgcgtgaac tttggctaac cacgactgac
    10441 ggctgctgtg aagcgtatgc ttggtggttt taatgctgtc ttttgaagat tcaaaaccca
    10501 agccggcacc cacacggaaa tttaaagaat cacttaacgt ttgggtgtag gtgtagcctg
    10561 tgtaaagatc gatacggttt tcaggaacgc cggtgggcag ttttacattt ttagtcttac
    10621 ccagcttgtt ctcatctgtt tccaaattaa taatattttt tttgctgcgc ccgaaaccgg
    10681 cttccaagcg gatgccttgg ttggcatcaa aaggaatatc agcacgtacg ccgatgtgtt
    10741 tggcagcttt gtgtttttct ttcaggaaag cacgagttga agaaatggaa gagaggtcgg
    10801 tgtggacggt aaactcatta gcggtttgaa gctcttgtgc agcggcggca gtaccggtca
    10861 gggcaatcat ggcacatgta aaaactgttt ttttcatagt taaaatctcc aaaatttgga
    10921 ttgtagtcgg atatggtaac ataacgtaaa taatcgttac gcttacaatt ata
//
LOCUS       DAXIHS010000069        10444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXIHS010000069 DAXIHS010000000
VERSION     DAXIHS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.069"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFOF2_001574"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFOF2_001574"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC7107859.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2843..3637)
                     /locus_tag="ACFOF2_001575"
     CDS             complement(2843..3637)
                     /locus_tag="ACFOF2_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC7107860.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYHLPSDLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKIHIGTIGEADDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGEGEFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAANTYL
                     MYLRFCLVSGRMDADEYEMEIKRMRNYLSAQVGEKPHWEEFVRAWE"
     gene            complement(4387..5265)
                     /locus_tag="ACFOF2_001576"
     CDS             complement(4387..5265)
                     /locus_tag="ACFOF2_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC7107861.1"
                     /translation="MYRDEFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIG
                     EINLAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLD
                     DALAAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNV
                     FLIGRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAAL
                     ANAKVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFW
                     LHKRFKTRPEGSPDFY"
     gene            complement(5306..5842)
                     /gene="ruvC"
                     /locus_tag="ACFOF2_001577"
     CDS             complement(5306..5842)
                     /gene="ruvC"
                     /locus_tag="ACFOF2_001577"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC7107862.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5845..6084)
                     /locus_tag="ACFOF2_001578"
     CDS             complement(5845..6084)
                     /locus_tag="ACFOF2_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC7107863.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6114..7124)
                     /gene="dusB"
                     /locus_tag="ACFOF2_001579"
     CDS             complement(6114..7124)
                     /gene="dusB"
                     /locus_tag="ACFOF2_001579"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC7107864.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
     gene            7490..8863
                     /locus_tag="ACFOF2_001580"
     CDS             7490..8863
                     /locus_tag="ACFOF2_001580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC7107865.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            9100..10125
                     /locus_tag="ACFOF2_001581"
                     /pseudo
     CDS             9100..10125
                     /locus_tag="ACFOF2_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT         2413 a         2883 c         2705 g         2443 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcctgc
      241 accaaacggg cggcgacctg cgggttgaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tgaacctgct gcagggtgtc gctgcggcgg cttgagccga taagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgcg caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcaagcgtgt ccaacaaggc ttcgcgcgcc tgatggtagc gcagcgggtc gatgttttcc
      841 gcgccgtccc acagctcggc ttcagacggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 agttcaatac cgtctgaaag tgcggcaagg ttggcggcga cggcacggcg gtagagcgtt
     1021 tgtgcggctt cccagcgcgt gaaggcgtcg ctgtcatggg cgagcagaag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcacc ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgcc ttcgagcagg aagatctgtt cggcttcggt cagcagcaat
     1201 acggcttcgg tcgcgcgttt gccctgataa tcgaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ctttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaaa ctattgtttt tcagacgacc ttccgcttcc
     1381 aagacgggcg tacccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtgtcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcag cccttccttc aacgaaagct ggaaccagtc gcggcaggtc
     1801 acgcggttgc ccgtccagtt gtggaaatac tcgtgtccga cgacagattc gatgccttcg
     1861 aaatcggtat cggtggcggt gcggctgtcg gcgaggacaa acttggtgtt aaagatgttc
     1921 aaacccttgt tttccatcgc gcccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcatcgcgtt tttcaaggat
     2041 tccacggcaa agccgacctt gggcttgtcc gcctcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaacggtct tccgtgaccg ccaagtcgcc cgcgaccaaa
     2161 gcaaacagat agctcggttt ggcaaacgga tcttcccatt tcacccaatg gcggccgtct
     2221 gaacactcgc cgccgtcgat tttattgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atcacatcag gacggtcgat gtagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacagat tgccgccgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggatggca cgcctgcaat cgtcagcgtc tcaccttcca acacataatc cgccgccgcc
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagtgg ctcccccacc
     2581 ctctgcggct cgaccgtcaa acgggatttc acgacggttt gcggttcggc aatatcgaaa
     2641 tgcaggtcgg tttcgaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 gttttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacggc
     2761 atgggcgcat cccgcgtatg ccatctgaag ccgcaacggc ggcacgggcg cgccgccgga
     2821 caaccgattt gaatttcaat ctttattccc acgcgcggac aaactcttcc caatgcggct
     2881 tttccccgac ttgtgcggac aggtaattcc gcatccgttt gatttccatt tcgtattcgt
     2941 ccgcgtccat cctgccgctg accaggcaga aacgcaggta catcagataa gtgtttgccg
     3001 cgtcggtttc gcaataattg cggatttcct tcagcctgcc cgtatggaac gcctcccaaa
     3061 ccttgctgcc gtccataccc agcttgcccg gaaaaccgca cagtttcgcc atatcgtcca
     3121 gcggcacgtt tgccctcggc tggtaaagcg cgagcaaatc catcaaatcg cagtggcgtt
     3181 ggtgatagcg gctgatgtag ttgttccact tgaaatcgcg gctgtcgcca aattcccctt
     3241 cgcccatatc ccaataacgc gcggcattga tgccgtatat cagggagcgg taatgcagta
     3301 cgggcaggtc gaacccgccg ccgttccaac tgaccaactg cggcgtatgt ttttccacca
     3361 actcgaaaaa tttggcgata accacttctt cgccgtcgtc cgcctcgccg attgtgccga
     3421 tatggatttt gtcctgcccc cagcgcatac agcacgaaac cgccacaacc tgatggagat
     3481 gatgctgcat aaaatccccg cccgtctggg cgcggcgttt ttgctgggca aacagcacca
     3541 cttcatcgtc gggcaaatcg gacgggagat gatataaagt ccgtatccct tgcacgtcgg
     3601 gcacggtttc aatatcgaag gccaaaatcg tattcatggc agtaccttgc attcaaaaac
     3661 agacttgcgc ctattgtgtc attaaaaggc ggataaaaaa agaggcaacc ccccacagga
     3721 ttgccccaat acctcaaatc agagatttac gcttcacaaa caatacaggc tttcgcctgc
     3781 ggctttaccc gcgtagctca actctacgcc ggcaaacttt cgtttcaccg tttccgatga
     3841 aaccccgacc aatcgcaaga ctgaccggaa aatcctttca gacggcattt cctgcctgtc
     3901 gtgtaattcc atgtagcgaa atgtacgcca ttttctacgc tttgccaagc attttttaca
     3961 atataaatgt caaaacatta attttataaa attgctgaaa atattaaata catgaatttt
     4021 tattttaaat attttaataa atataaattt aattttgatt tatatttaaa tttaagcata
     4081 aaatgttaaa tattaaagga agcatgaaag gcatatatta aatatttatt tataacgcta
     4141 tgtttttaaa gaaaattaat tttaatatat taaccagatt gtctgcatat attcatgggt
     4201 ttgcggtatt tcttccaaaa cctgcttcga atttcccgac caagtcttaa aaatattgtt
     4261 tttgagatac ttaaatagca gcgattatca aatgaaatct gttcatataa tctgccattt
     4321 cgcatttaaa aaaacaatca gtagtttcga ctcgaaacgc ctgatatgtt ttgtaatttt
     4381 acgtagtcag taaaaatcgg ggctgccttc cggacgggtt ttgaaacgct tgtgcagcca
     4441 aaaatactgc tcgggatgtt cgcgcacgcg ttcctcgata aaacggttca tgcgctgcgc
     4501 gtcggcttgc gcatcttcac tcggaaagga tttccaagca gggtagaaat gcaatgtaac
     4561 cgtattgtct gcctcgcgga caggaatggc gggtatcact tttgcatttg caagcgcggc
     4621 aatgcggctc aagccggtaa tcgttgccgt ctgaatcccg aaaaaatcca caaaaaccga
     4681 atcgttgcgt ccgaaatcct gatcgggcag atacagaaac ggtgcgctgc ttttgcggaa
     4741 ctgtttgacg agggcacgca gcccttcggt gcgcccgatg aggaaaacgt tgtgatagcg
     4801 gtttcggcct ttcaaaatct gttcgtccaa tatcttgttt ttttgatggg aatacatact
     4861 gatcagcggg acatcctgat taagcgcgta taccgccatc tcgaacgcgg taaagtgcgg
     4921 atacaggatg atgacttttt cccccgccgc cagcgcgtcg tccaaataat gcttattgcg
     4981 gtagcgcacc agcgatttca aacgcccggc aggcgcgtac caatataaac cgtattccaa
     5041 catcaacttc gccatgtgtt tgaaatgctg tttcaacacg gttttacgct tttcctcact
     5101 ccattcggga aaacattttg ccaaattgat ttcgccgata cggcggcgcg gtttgaccag
     5161 aaggtaggca agcaaacccg tcaggtcggc aatcttgtgc agcagcgcaa acggcagaaa
     5221 ctgcaaaaca tacagtacaa aaaatataaa ttcatctcga tacacatttt cttttcagac
     5281 ggcaaaatac aaatgccgtc tgaaactatt gaaaccttcc gcgcttgacc tgcatccctg
     5341 aaggattgag tttggcggca agcccgtggt tgcgtaaggc gtgggtcagc gcgacggcaa
     5401 gaccgtccgc cgcatccgcc tgcggtgttc ccgaaagccc cagcatctgc accaccatat
     5461 gctgcacctg ttctttcgct gccttaccct tgccgaccac cgcctgtttg acctgcaagg
     5521 ccgtgtattc cgaaacgggc agcttatggc tgaccaatgc cgccaatgcc gcgccccgcg
     5581 cctgaccgag catcagcgtc gatgcaggat tgacgttgac gaacacctgt tccaccgccg
     5641 cctgttgagg cttgtaaacg gtaacgactt cgccgatgtg ccggacgatg acggcaatcc
     5701 tgtctgccag aggcgcatcg gcaggcgttt tgatgcagcc tgaggcaacg taaaaatgat
     5761 cgcgccccct gacatcgatg atgccgaaac ccgttacgcg gctgcccggg tcaatgccca
     5821 agatacggac ggttgccgac atattcacag caaaccgtgt tgaatcagtt ttttacgcaa
     5881 ggtattgcgg ttcagtccca acatgacgga ggctttggac tgattaccgc cgcattgctc
     5941 catcacgcac accagcagcg gcttttccac ctgatgcaat accatatcgt acacgccgca
     6001 aggttcggta ccgttcaggt ctttgaaata ttgttctaaa ttttgtctga tgcattggga
     6061 aatatcggga agggtatggg gcatgattgc actttcaaag gataatcaag tgttcagaag
     6121 gcatttgggc ggtaggcgca cgcccaactg tcggtttttt cggcaagtct ttcaagataa
     6181 cctgcaagca tgtcgtattg cgccgccgca ctgtccaagc ggttgatttc acgacgtgtc
     6241 tgttcgccgt cgggcatttc gtcgatgtac cagcctatgt gtttgcgtgc gatgcgcaca
     6301 ccggcggtgt cgccgtaaaa cgcgtgtatg gcgcggatgt ggttcaaaat agcggcggcg
     6361 cattctgcca aactcaaggc aggcggcaaa acaccgtgtt cggcataatg tttcaaatcg
     6421 cggaagaacc acggcctgcc ttgcgcgccg cgccctatca taatgccgtc ggcggcggtt
     6481 tgtttgagga cggcttgggc tttttgcggc gaagtaatgt cgccgttgac ccagaccggg
     6541 atgttcagac ggcatttggt ttcggcgatg agttcgtaac gcgcttcgcc tttgtacatt
     6601 tgcgtacgcg tgcgtccgtg gacggcaagg gcggcgatgc cgcaatcttc ggcgattttg
     6661 gcgatgacgg gcaggttttg atggtcgtcg tgccaaccca aacgggtttt gagggtaacg
     6721 ggtacgcctg ccgcacggac gacggcttcc aaaatggcgg caaccagcgg ctcgttctgc
     6781 atcagcgcgc taccggcttg gacattgcag acttttttag cgggacagcc catgttgatg
     6841 tcgataagct gcgccccaag gctgacgttg taacgcgcgg catccgccat ctgctgcgga
     6901 tcgcttccgg caatctgcac ggcaacaatg ccgccttcat cggcaaaatc gctgcggtgc
     6961 aaggtttttc tagtatttct gagcgtcggg tcgctggtca gcatttcgca caccgcccaa
     7021 cctgcgccaa aatctcggca aagtcggcgg aacggtttgt cggtaatgcc cgccatcggc
     7081 gcaagtgcga tggggttgtc gataaaatag ccgccgatgt gcataatgga tccgcgtttc
     7141 aaaaaagtac gccattgtac attttttaag caggatttcc aatctccgga cgcgcccgcg
     7201 attgggtcgg acaccgtttt atggcataat ccgcacacag attccctgcc ccgccactca
     7261 caggcgggca gtttatagtg gattaacaaa aaccagtacg gcgttgcctc gccttagctc
     7321 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggtttcgt gctatttgta
     7381 ctgtctgcgg ctcgccgcct tgtcctgatt tttgttaatc cactatattt ccccgtccta
     7441 tcggtttccc gtttcagacg acataaggtc tgaaagaaag actacaatta tgagtaatcc
     7501 attttcctct ttaggtttgg gtacggaact cgtttccgca ctgaccgcgc aaggttacga
     7561 aaacccgacg cccatccaag ccgccgccat tcccaaagca ctcgccggtc atgatttgct
     7621 agccgccgcg caaaccggca caggcaaaac cgccgccttt atgctgccca gtctggaacg
     7681 cctcaaacgt tacgccaccg ccagcacctc gcccgcgatg caccccgtgc gtatgctcgt
     7741 cctcaccccc acgcgcgaac ttgccgacca aatcgaccaa aacgtgcagg gctacatcaa
     7801 aaacctgccg ctgcgccaca ccgtcttgtt cggcggtatg aatatggaca aacagaccgc
     7861 cgacctgcgt gccggctgcg aaatcgtcgt cgccaccgtc ggacggctgc tcgaccacgt
     7921 gaaacagaaa aacatccatt tgaacaaagt cgaaatcgtc gttttggacg aagccgaccg
     7981 tatgctggat atgggtttta tcgacgacat ccgcaaaatc atgcagatgc tgccccgcca
     8041 acgccaaacc ctgctctttt ccgccacctt ctccgccccg atacgcaaac tggcgcaaga
     8101 cttcatgaac gcgcccgaaa ccgtcgaagt cgccgcgcaa aacaccacca acgccaacgt
     8161 cgagcagcac atcatcgccg tcgataccat tcagaagcgc aacctgctcg aacggctgat
     8221 tgtcgatttg catatgaacc aggtcatcgt gttctgcaaa accaaacaaa gcgtcgaccg
     8281 cgtaacgcgc gaactggtgc gccgcaacct gtccgcacag gcgatacacg gcgaccgttc
     8341 ccaacaaagc cggctcgaaa cactcaacgc cttcaaagac ggcaacctgc gcgtcctcgt
     8401 cgccaccgat atcgccgcgc gggggctgga tattgccgaa ctgcccttcg tcatcaatta
     8461 cgaaatgccc gcccagcccg aagactacgt ccaccgcatc gggcgcacgg ggcgcgcggg
     8521 cgcggacggc gtggcgattt ccctgatgga cgaatccgaa cagaaaatgt ttgaatccat
     8581 taaagagctg accggcaaca agctgctcat cgagcgcatc gagggcttcg agccgcaatg
     8641 gtgggaacag ggcggcgcaa aaccggaaaa acccgaaatg cgcgaaccaa gacaacgcaa
     8701 ccgctacgaa tccgccaaag cgcaacgcga aaaaaacacc cggccggaaa atgcggcaaa
     8761 cgatgcgggc gcggcttgcg gaaaaattgc cggacgcagc cgccgaagcc gccgggaaca
     8821 ccggacgtgc gccctgctcc aaccgcgtta cggcgtaaaa taaccctgaa aatcaaatgc
     8881 cgtctgaaca tttcccgttt cagacggcat ttttcaaacc ggactgacgc attgggcgca
     8941 accgcccgca ccggataaat ttctgccgca aacagtttca gacggcattt gccgcctgta
     9001 caatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     9061 tacggaaccg attcacttgg tgcttcagca ccctagagaa tcgttctctt tgagctaagg
     9121 cgaggcaacg ccgtactggt ttttgttaat ccactatatc aagtctgcgt gttggaaaaa
     9181 cagcactttc cgcgcttcgt catcggcgaa agcctgctgc cgcactgtat ggaaatgctg
     9241 gaagaagccg gatttgccga tgccgtgcgc gccgagcccg gctttcagtt gaaaaacggc
     9301 gcggcgtttt cgtggggcag ccgttatacc gaattcgatt tcaccgataa attttcagac
     9361 ggccccggca cgacttatca agtgcgccgc gccgtgttcg ataaaatcct gattgaagaa
     9421 gccgccaaac aaggcgttga agtacgtttc gggcacggcg taaccgcgtt cgacaacagc
     9481 ggcgattttg tccgcctgaa catcgaaacc gacaccggca agcgctatga actgaccgcg
     9541 aaattcgtct tggacgcaag cggttacgga cgcgtgctgc cgcgcctgct aaacttggaa
     9601 acgccctcgc acctgccgcc gcgccaagcg catttcacgc acatcgacga caacatcacc
     9661 aacccgaaat tcgaccgcaa caaaatcctg attaccacct atccgcaaca ccgcgacgtg
     9721 tggatttggc tgattccctt cggcgacaac cgctgttccg tcggcgtggt cggcacgccc
     9781 gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat ttgtttacga atgcccgatg
     9841 ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt ttccgttccg ctccatccaa
     9901 ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt tcgcgctatt gggcaatgcc
     9961 gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca tcgcgctgca ctccgccaaa
    10021 cttgccgccg atctgttggc aaaacaactc aaaggcgaag ccgccgattg gcaaaccgaa
    10081 tttgccgaac ccttgatgat cggcgtggac gcgttccgca cctatagtgg attaaattta
    10141 aatcaggaca aggcaacgaa gccgcagaca gtacaaatag tacggaaccg attcacttgg
    10201 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtactggt
    10261 ttaaatttaa tccactaaat agtgtatatt aagtacgtct gatatacgcg ataccctccg
    10321 agggtgtaaa ctttagttca catttaaaat gacctcttta aacctgtctt tcggcaggtt
    10381 tctttttagg ttgtttggaa atcgtgtgca gacaaggtgt aaaataggta acaacataaa
    10441 ataa
//
LOCUS       DAXIHS010000070        10031 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXIHS010000070 DAXIHS010000000
VERSION     DAXIHS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.070"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(597..830)
                     /locus_tag="ACFOF2_001582"
     CDS             complement(597..830)
                     /locus_tag="ACFOF2_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC7107866.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(996..1196)
                     /locus_tag="ACFOF2_001583"
     CDS             complement(996..1196)
                     /locus_tag="ACFOF2_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC7107867.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1368..2243)
                     /gene="xerD"
                     /locus_tag="ACFOF2_001584"
     CDS             complement(1368..2243)
                     /gene="xerD"
                     /locus_tag="ACFOF2_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC7107868.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2295..2735)
                     /locus_tag="ACFOF2_001585"
     CDS             complement(2295..2735)
                     /locus_tag="ACFOF2_001585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC7107869.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            3056..3994
                     /locus_tag="ACFOF2_001586"
     CDS             3056..3994
                     /locus_tag="ACFOF2_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC7107870.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            4123..4416
                     /gene="hfq"
                     /locus_tag="ACFOF2_001587"
     CDS             4123..4416
                     /gene="hfq"
                     /locus_tag="ACFOF2_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC7107871.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4501..5067
                     /locus_tag="ACFOF2_001588"
     CDS             4501..5067
                     /locus_tag="ACFOF2_001588"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7107872.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            5121..5495
                     /locus_tag="ACFOF2_001589"
     CDS             5121..5495
                     /locus_tag="ACFOF2_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC7107873.1"
                     /translation="MQYLIVKYSHQIFVTVTILLFNIRFFLLWKNPEKPLAGLWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSVKFYAV
                     YLLAICCVACIVYLAKTKVLPF"
     gene            5505..5999
                     /gene="folK"
                     /locus_tag="ACFOF2_001590"
     CDS             5505..5999
                     /gene="folK"
                     /locus_tag="ACFOF2_001590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC7107874.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRERSFRNAPRTLDLDII
                     DFDGLSSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDK"
     gene            complement(6126..6605)
                     /locus_tag="ACFOF2_001591"
     CDS             complement(6126..6605)
                     /locus_tag="ACFOF2_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC7107875.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6825..7562)
                     /gene="ubiE"
                     /locus_tag="ACFOF2_001592"
     CDS             complement(6825..7562)
                     /gene="ubiE"
                     /locus_tag="ACFOF2_001592"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC7107876.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7605..7973)
                     /locus_tag="ACFOF2_001593"
     CDS             complement(7605..7973)
                     /locus_tag="ACFOF2_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC7107877.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            8093..9712
                     /gene="mnmC"
                     /locus_tag="ACFOF2_001594"
     CDS             8093..9712
                     /gene="mnmC"
                     /locus_tag="ACFOF2_001594"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC7107878.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITPAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9826..9943)
                     /locus_tag="ACFOF2_001595"
                     /pseudo
     CDS             complement(9826..9943)
                     /locus_tag="ACFOF2_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2683 a         3050 c         2014 g         2284 t
ORIGIN      
        1 tgctcgccag cctactaatg aaaaactgaa ataacaaggg gtggcaaatt accgcgcccc
       61 tttcgccaac ctttcttcga gccattttac tacaatggtt ttaaaaatca aggattgcct
      121 gatgccgccc gcccctctgt ttcctccgtc aagcaccgcc ttggcggttt tcttatatag
      181 tggattaaca aaaaccagta ctgcgttgcc tcgccttgcc gtactatttg tactgtctgc
      241 ggcttcgtcg ccttgtcctg atttttgtta atccactata aaatctaccg attacacaaa
      301 cacatatcat ctttacacaa tcatgcttcc atcaacagta aaacatgata tgattgccaa
      361 caataacatc tcacaataaa ttttctaatt tttattgaaa aaatcaataa ataagaattc
      421 tcccaaccga caaataaaat aaagaaaggg ttaatatgca acatcgaaga agactagcaa
      481 tttaccaagc atccaaacgt gcttccttta ccggcaggtc atcagccccg caaaaacgta
      541 aagaacgtta atttgaaaaa aatgccgtct gaagtcctgc ttcagacggc atttttttac
      601 cgttcgagaa actgtttcaa cctgtcctca tccaaaaacc agtggcagat ttcctcatct
      661 cccgccaaca aaacgggaac cagctcattg tatttttctt ccaaaacagg attttcatcc
      721 acatcgacca cttccagccc gaacccgtat tcattttgaa aaggtttgag tgcatcgcgc
      781 attttgtggc acaagctgca atattcacga aacatcaagg tcaatttcat ccgcgtttcc
      841 ttatctgtca atttgcacac gccaaagcct tagacgcagc agaatcatgg tctatttggg
      901 aaaaaacaat gttttcaagg aagatgatac tcaagtcctg ccaaaaacag caaaaatgcc
      961 gtctgaacag ttcagacggc atttccaaaa ccgttttacg cttgaacatt gatacccgcc
     1021 gtaaccgtcg gttgcgcatt gtgcgccgtc agaaacgaag cggcaagctc cccacagcag
     1081 ccgcagcagc tcgctacgcc caattgcgac tgcaaatcgc ccattgtggt cgcgccggcg
     1141 gcgatggttt ccttgatttg atggtcggta acggcattgc agatgcagac aaacattttg
     1201 tgctccgtgt gttctcaaac tatcgtaccg atagcggctt ttattatcgt atgcgaatat
     1261 aaataaaaac ggttcgcatt gcaaggtcgg tatacacggt ttgtcttggt aattttttat
     1321 caagtttgca ttttcgattg atttcttatt aaaccaaagt agaagcctca gtttcgggaa
     1381 tggtgttcct tcactacacc ctgcaaccat acgttggcaa catgggtata aatctgcgtc
     1441 gtattcaaat cggcatgtcc caacatatcc tgaaccacgc gcaaatccaa gccgtgccgc
     1501 accagatgcg tggcaaaggc gtggcgcagg ctgtgcgggc tgatgtgccc gatgcctgcc
     1561 tgacttgcat attctttgac aatcatccat gccaactgac gggaaatgcc cgtctttttc
     1621 tgactgacaa acaatgcgtc gcaattcctg cctttcagca gaagtgggcg tgcctccgta
     1681 taatagcgtt ccacccaata cgccgactcc tgccccatcg ggaccatcct ctgcttatca
     1741 ccctttccca gcgcggtaat acagcccctg tccaaatcca cattgccgaa gttcagcccg
     1801 accgcctcgc tgacgcgcaa gccggtcgcg tacatcaatt cgagcaaagc cttatcccgc
     1861 aaaccgtgcg gcgtgtcggt atccggggcg gcaagcagtc gggaaatctg ctgctcggtg
     1921 atcagggtcg gaatattctt gtcgattttg ggcggtttca gcaggcaggt gggattgtcc
     1981 gtccttatgc cttcacgctc catccatata tacaggcgtt tgcacgccga taatgcgcgc
     2041 gcctgcgaac tccgttgctc tccgtcaaca taaaccgccg ccgccaaatc cgcttcgtcc
     2101 gcatccttca gcgttctgcc cgattgggac aggcggcggg cgattttttc caaatcgcgc
     2161 cggtaaccgt ttaaagtatt ctgactgagc cgcctgtcca accacagcgt ttcaagcagc
     2221 ctgtcgatca aaccttcttc cataccgttc caaacaaatg ccgtctgaat cttcttcaga
     2281 cggcatggtt cacattatcg ggaaagcgtt tccaatactt cctgcgcgtg acccgccact
     2341 ttgactttcc gccattcatg ggcgatttct ccatccttat tcaagacgaa cgtactgcgc
     2401 tcgataccta acgactcttt cccgtacagt ttcttcaatt tgatgacatc aaacaggcgg
     2461 cacactgttt catccttgtc gctcaacagc tcgaaccgga aaccctgctt ggcgcaaaaa
     2521 ttctgatgcg cctttacgcc gtcgcgggaa ataccgacca cggtataacc caatgcctca
     2581 aactgttcca aacgcgcatt gaaatccaag ccttccgtcg tacagccagg cgtactgtct
     2641 ttcggataaa aatacacgac caaaggcaga tgttctgccg aatgaaaatc cgcaccgctg
     2701 ctcgaaggca gggtaaattc atatttcaca tccatagtcc tactcccgat attcccatta
     2761 ttcaaaacgg cacgcagacg caccgccgca attgccaaac caaccccgat tctaccgccc
     2821 caaaggacaa ggattcaacc gccggaaaca tccaaaccga cacacgacgg catgaaaaat
     2881 atccatgtca aaccacaaaa tatgttccga tttaaaaaca gaatgttata aaacccaatc
     2941 cccaaaacac aacaagaccg cccgcctacg ggcagtctcc tgtcagacga catactttac
     3001 agatggctgt tttttcaaca aaataacgcc aatactcaaa aatatggaat caaaaatgtc
     3061 catccatact ctgaaacgcc tgccctcatc gctgctgctc ggtctctgcc tttccctgcc
     3121 gtcagcccac ctttttgccg acaacgacat tttagggcaa tttttagaac agaacatgct
     3181 tacctcctcc gatccgatag aaatattcgc cgaaagcaca atacacccca ccaacaccca
     3241 agccattaca ggcggtctga ttctctcctc acagtctgcc ctggtcgtca acaacaaaac
     3301 cggacagata ctgtatcaga aaaacgccga caggattatg cccatcgcct ccatttccaa
     3361 actgatgagc gcgatggtcg ttttggatgc aaacttggac atgaacgaaa ccgttaccat
     3421 tacgcccgac gaaatcgacc gcatcaaagg gaccggcagc cgtcttgcca taggtacggc
     3481 acttacacgc aaagaactgc tgcacctgag cctgatgagc agcgaaaacc gcgccaccca
     3541 tgcattgggc agaacctacc ccggcggcat gggcgcattt gtcgccgcca tgaaccgcaa
     3601 agcccaaagc ctcggtatgt acggcagccg cttttacgaa ccgaccggac tcaacttcca
     3661 aaacgtttcc accgccaaag acctgagcct tatggtcaac gccgccgccc aatatccgca
     3721 aatccgcacc aactcgactt ccaactacgc ctcggtacag accaaaaacg ggcagcagaa
     3781 ctacaaaaac tccaatgccc tggtcagaga aggcatgtgg aacatcgaat tgcagaaaac
     3841 cggctacata cgcgaagcag gcaggtctat ggttgtcaaa gccaacattc aaaatcaacc
     3901 cgttaccatc gtattgctga actcgcccac atccgccaca cgcgtcaacg acgcccgcaa
     3961 aatcgaatcg tggatgctgc agcaacgctc ctgacataca aatgcccggc ggaaaaccgc
     4021 ccaaacacaa aaaaaattgg taatttttcc tattcaaggc tacaattcga cacgcacagg
     4081 gcgtctgccc gatttcacaa tcataataac ggagttaaaa atatgacagc taaaggacaa
     4141 atgttgcaag atcccttcct gaacgccctg cgtaaagagc acgttccggt ttcgatttac
     4201 ttagttaacg gtatcaaatt acaaggtcag gttgagtctt tcgatcaata cgttgttctc
     4261 ctgagaaaca cttccgtcac ccaaatggtt tacaaacacg ccatttccac catcgtaccg
     4321 gcacgctccg tcaacctaca acatgaaaac agaccccaag ccgcaccgac ttcgaccctc
     4381 gtccaagtgg aaaccgtcca gcagcctgcc gaataatccg cacgaagcat gacgtgtcat
     4441 atctttcaat accttaccgg acaacggtaa ggtattttta ttttcagaca gcatttaaaa
     4501 atgttattgc aaaacatcct tccattcgcc cattgccttt tgcggaaggc acttcccgaa
     4561 ggtggcaatg ctttggacgg caccgccggc aacggacacg acaccctttt cctcgcacaa
     4621 accgcaggca tccgggggaa agtgtgggca ttcgacatcc agccgcaagc cctgagccgc
     4681 actcgatgcc gtctgaaaga agcgagatac gagaatgtgc aactcatctt agacggacac
     4741 gaaaacctga aacaatatgt ttcagaacca ctaaatgccg ccattttcaa ctttggctgg
     4801 ctgcccggcg gagataaaag ccttaccacc catacggaaa ccagcattcc cgccctttgc
     4861 gcaaccctct ccctgctgaa agaaggcggt atgcttattg ccgtcctcta tccgggacac
     4921 gaaaacggca aacaggaggc agaagcaatc gaacaatggg caaaaaacct gcctcaagaa
     4981 cagtttgccg ttttgcgtta cggctttacc aaccggaaaa acaacccgcc ctatctgttg
     5041 gcatttgaaa agttacgtca aaaataaatg tttgcggtaa aataagccga ttccaaccct
     5101 tattttgata agagccgact atgcagtatc tgattgtcaa atacagccac caaatcttcg
     5161 ttaccgttac cattttgttg ttcaacatcc gctttttcct gctttggaaa aatcccgaaa
     5221 aacctttggc gggcttgtgg aaggcactgc cccacctcaa cgacacgatg ctgctgttta
     5281 cgggattgtg gctgatgaaa atcacccatt tctccccgtt caacgcgcct tggctcggca
     5341 caaaaatcct gcttctgctc gcctatatcg cattgggtat gatgatgatg cgcgcccgcc
     5401 cccgctcggt caaattctat gccgtttacc tgcttgccat ttgttgcgtc gcctgtatcg
     5461 tttaccttgc caaaaccaaa gtcctgccat tctgaaacac tgccatgaac aacagacatt
     5521 ttgccgtcat cgccttgggc agcaaccttg acaaccccgc acaacaaata cgcggcgcat
     5581 tggacgcgct ctcgtcccac cccgacatcc ggcttgaaca ggcttcctca ctgtatatga
     5641 ccgcgcccgt cggttacgac aatcagcccg atttcgtcaa tgccgtctgc accgtctcca
     5701 ccgatatgga cggcatctcg ctgctctctt tgctcaaccg tatcgaggcg gatttcgggc
     5761 gcgaacgcag tttccgaaac gcgccgcgca cattggattt ggacatcatc gactttgacg
     5821 gcctatccag cgacgacccc cgcctcaccc tgccgcatcc gcgcgcacac gaacgcagtt
     5881 tcgtcatacg ccctttggca gaaatcctcc ctgattttgt tttaggaaaa cacggaaaag
     5941 ttgccgaatt gtcaaaacaa ctgggcaatc aaggtatccg tcttttaccg gataagtaat
     6001 tccgcacgcg gatgccgtct gaatgccggt ttcagacggc atttttcctt tgccgccaac
     6061 acgcgtgcaa aaaaatcgcc ccttggaaaa ggggcgcaaa aggaacacaa accactacca
     6121 aaactttaaa tctgcaacat tgcctgccgc atactgtatc cgacaggata taaagccctc
     6181 actaaatcgt ttcgagaaat ccaaacttct tcatcgccga cagaaaatct gcctttctcc
     6241 ggcaccagct ccaacaaaaa cggttgaacc gccgtatgca gcctgtcctt acaccgcccc
     6301 agctttctgg acaaggcgga cagggcacgc ttatagacac tatccttgcg gtcaagttcc
     6361 cgcgcactgc cgacccagct caaacgaagg ttgcggcttt catcctcaat cagcaaaatg
     6421 cctgttttga cttcccccac ctctggctcg catgaaagcg aaatataata tttgccgtcg
     6481 atgttgacat acgccgcctc atgcacctga cagttgcagc cggaagtggc aagcagcgaa
     6541 taaacagaat cccgatgcag tttcaaaata tcaagggaaa tctgtttgaa aatactttcc
     6601 ggcatttttc cctcctttcg tctgctgcgt tgtttactgg attgactgca ttatacagac
     6661 atctcccaat cttgcaaatg ataattgttc ctatttttta aaaaatttat aaattattga
     6721 tagatatgtg aattaatttt aagatttaaa atctctttct caaaaaaaca caaaaatgcc
     6781 gtctgaaacg tgttcagacg gcattggctg cacaggcaat ccgtttaaaa tttcacgccc
     6841 ttatgcagcg caacgatccc cgcactcata ttatgatagt ccacactgtc aaatccggca
     6901 tccagcatca tttgctttaa agtttcctga tcggggtgca tacggatgga ttcggcgaga
     6961 tactggtagc tgtccgcatc tttggcaatc agtttgccca tgaccggcag cagcttgaac
     7021 gaatagaaat catacgcgcc ttcgagcggc ttgtagattt tggaaaactc caacaccagc
     7081 aacgtgccgc caggtttcaa aacacgatac atctctttca gcgcggcatc tttatgcgtc
     7141 atgttccgca agccgaacgc cacggaaact aagttgaaat aattgtcagg gaaaggcagt
     7201 ttttccgcat cggcaagcga cacgggcaaa atcatgcctt cgttcaacag acggtcgcgc
     7261 ccgacggtca gcatagagga attaatatcg gtcagccaaa cctcgccttc cttgccgacc
     7321 cgtttcgccc aaccgcgcga caaatcgccc gtaccgcccg caatgtccaa cactttatcg
     7381 ccttttttca ggtgcgccgt gttgatggtg aaatgcttcc acaccctgtg cagccctgcc
     7441 gacatgacat cattcataat gtcgtagttt ttggcgacgg agtggaacac ttccgccact
     7501 ttgccggctt tttcatcttc gttgaccgtg ctaaacccga aatgcgtttt ttgtccgccc
     7561 atattctaac cacctccctg ataccgccct acggcatccc aaacttaaca atctgattgc
     7621 ttttcaaaac gcgacgcgcc cgccgccgcc aatttgtcca aatattcctg ccacatcgca
     7681 tcgtatccgt atgccagtct gtgcagatac gcccaatcgt aaagaccgct gtcgtgcccg
     7741 tccgaaaaac tgattttcag cgcgtactgt ccgacaggct gcaaatccgt aatttgcaca
     7801 tctgccttac cggtctgcaa aacatcctgt cccacaccgt gtccgcgcac ttccgcactc
     7861 ggcgaataga cgcgtaaaaa ttcggcaggc agatttttag gttcgtcccc ataagtcaaa
     7921 accaacacgg tacggttttt caacaaacgg atttcaacag gaatattatt caaaaaacac
     7981 ctcggttcgg catacaatat ccggcaggcg caggcgtgcc ttcaaacggc gcgtatttta
     8041 acagctttat cccgataccg atattcaaac ccacacttta aacggcattg atatggacaa
     8101 tctcgtatgg gacggcgttc ccgacatccg cacactcgac caaaccatcc gcaaacacgc
     8161 acacccgctc aacctgattg tctgcctccc cgataatcag attcccgatt ttcaaaccgc
     8221 acaagatgct tcggacgcgg aatgccgtct gaagcaccgt ttggatcagg caacgcagtg
     8281 cctccagttc gacagcatca acctcatcga acacatcctg cccgatgtcc gcttctggct
     8341 ggttccccct tcacgcaccc gccgcctgca cgaacacttc caccacattt cctggcagac
     8401 cgaagccatc ccgcaaaccg aaagcaaacc cgacaaaccc tggtttgcac ttccacaaac
     8461 atccgaacgg caaaaaccgg aacacatcct cgttatcggc gcgggcatat ccggcgcggc
     8521 aaccgcccac gccttagcat cacacggcat ttccgttacc gtattggaag cccgaaaagc
     8581 cgcccaagcc gccagcggca accgccaagg gctgctctac gccaaaatct cgccgcacga
     8641 caccgaacaa accgaactgc tgcttgccgg ctacggctac accaaacgcc tgctcggaca
     8701 catcctgccc gaatccgaaa cctggggcgg caacggcatc atccacctca attacagccg
     8761 caccgaacaa caacgcaatc acgaattggg tttgcaaaaa caccataacc acctctaccg
     8821 cagcatcacg ccggcagaag ccgaaaaaat cgccggcatc ccgctgaaca cgccctacgc
     8881 cgaaccatta tgcggactgt tttggcagta cggcgtatgg ctcaatccac ccgcattcgt
     8941 ccgcaccctc ctcaaccatc cgctcattgg actacacgaa gacacaccgt taaccgacat
     9001 ttcccacgac ggggaaaagt ggattgcaag cacgccaaac ggcatatttt ccgccacaca
     9061 catcatctac tgcaccggcg cgcacagccc tcgcctgccc gaaaccaacc tcgccgccct
     9121 gcctctcagg caaatacgcg gacaaaccgg cctcacaccg tccaccccgt tttccgaaca
     9181 actgcgttgc gccgtttcag gcgaaagcta catcagcccg tcgtggcacg gactgcactg
     9241 ctacggcgcg agttttattc ccaacagcag ccataccgga tggaacgaag ccgaagaagc
     9301 ctcaaaccgc caagcattgg cacaccttaa ccccgccctt tccgaatcat tgtttgccgc
     9361 caacccaaac ccccaaaaac accaagggca cgccgccata cgctgcgaca gccccgacca
     9421 ccttccccta gtcggcgcac tcggcgacat tgccgccatg cggcagacct acaccaaact
     9481 cgcgctggac aaaaactacc gcatcgacac cccatgccca tacctgccta atgcctacgt
     9541 caacaccgcg cacggcaccc gcggactcgc caccgccccc atctgcgccg ccgccattgc
     9601 agcccaaatc ctaggcttgc cccatccctt ttcacaacgc ctgcgccacg ccctacaccc
     9661 caaccgcacc atcatccgcg ccatcgtcag aaggaaggat ctaacccctt aagcctttgc
     9721 cgcatgccgt cccgaccaca gaaaatcata ttcaaataaa cataccaatc ctgacggtaa
     9781 aatatcgaaa acacccataa cgttttcttc ccaaaataag gagtaaggaa tgcctaacaa
     9841 attgcctttg ctgtccaaga tgaatgttcc gtaacattcg aagacttcct taaccctgcc
     9901 agccgtttcg gtttctgtac cccctgttag ataaggaagg aatgaaaaag ccctgttgat
     9961 tgtatgaagt aaagggcgtt aatttttgtt aatcttccct tctcaggaac tcaatatata
    10021 aggatgttaa t
//
LOCUS       DAXIHS010000071         9979 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXIHS010000071 DAXIHS010000000
VERSION     DAXIHS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.071"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(147..965)
                     /gene="hslO"
                     /locus_tag="ACFOF2_001596"
                     /pseudo
     CDS             complement(147..965)
                     /gene="hslO"
                     /locus_tag="ACFOF2_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            1210..1965
                     /locus_tag="ACFOF2_001597"
     CDS             1210..1965
                     /locus_tag="ACFOF2_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC7107879.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            1984..2202
                     /locus_tag="ACFOF2_001598"
     CDS             1984..2202
                     /locus_tag="ACFOF2_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107880.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            2347..2691
                     /locus_tag="ACFOF2_001599"
     CDS             2347..2691
                     /locus_tag="ACFOF2_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC7107881.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            2691..3383
                     /locus_tag="ACFOF2_001600"
     CDS             2691..3383
                     /locus_tag="ACFOF2_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC7107882.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            3450..4670
                     /gene="argJ"
                     /locus_tag="ACFOF2_001601"
     CDS             3450..4670
                     /gene="argJ"
                     /locus_tag="ACFOF2_001601"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC7107883.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            4730..6132
                     /locus_tag="ACFOF2_001602"
                     /pseudo
     CDS             4730..6132
                     /locus_tag="ACFOF2_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6321..7712
                     /gene="hrpA"
                     /locus_tag="ACFOF2_001603"
     CDS             6321..7712
                     /gene="hrpA"
                     /locus_tag="ACFOF2_001603"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC7107884.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            7813..8940
                     /locus_tag="ACFOF2_001604"
     CDS             7813..8940
                     /locus_tag="ACFOF2_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC7107885.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQVGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKIATEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            9118..>9979
                     /gene="lpt3"
                     /locus_tag="ACFOF2_001605"
     CDS             9118..>9979
                     /gene="lpt3"
                     /locus_tag="ACFOF2_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC7107886.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGG"
BASE COUNT         2333 a         3045 c         2461 g         2140 t
ORIGIN      
        1 tacctgatcc aacttcaata gcgggaaacg gtcgatgtgt ttggcaatca tggcttgggc
       61 ggtttgttga aagaaggtgc tcatgagaaa tcccctaaat gtcttggtgg gaatttaggg
      121 gattttgggg aattttgcaa aggtctcttg ctgtggcaga aatcgcaatc gacttcgatg
      181 ctgccttgtt ccaccaccac gccgccgact tcttccccgc ccagcatcag cagcatatcg
      241 ctgactttgc cgcgcgaaca ggtgcatgaa aattcaaacg tttccggctc gaacacgcgc
      301 ggcggcgttt cgtggaacag gcggtacaaa acatgttgcg catccaattc tgccagctcg
      361 tccgccgtca gtgtacgcgc cagcgtactg acgtgttccc atgcctcttc atccaacacc
      421 gcttcaggca gacgctgcac cagcagaccg cccgccgctt cgtcgcttgc agacaggacg
      481 atgtgcgtat cgatttgttc ggaacgcttc atatagttcg ccaacatttg cgcgataccg
      541 ccgccttcca aaggcactac gccctgccag ggttcgccgt ctttgggctg cagcgtcagc
      601 acgaatacgc cgccctcgcc caaaaggtcg ccgaggcttt cgtcatcggc tatttctgcg
      661 gtttcgtccc aacgcgcggt tgcacggacg gtacggtcgg aagccgcttc cgcaaccagc
      721 attttcagcc gcccccgccc ctgaacctgc acaatcagcg tgccttcgtt tttgaggttg
      781 cccgacagca acacacccgc cgccaacaac tcacccaaag cgcggcggat ggcggcggga
      841 tagtttttct gttttacaat gtgctgccac acgttttcca gacggacgtg cagcccgcgc
      901 acgggcatat cgtcgaagat aaagcgggta cgcacatcgg cgcggttgat ggcggtttga
      961 ttcatgattt tctctgactg attgttcgga tggcggctat atggttgcgg tcggcgcgaa
     1021 aacaagacgg acggcggatg cgcttcccaa attatcaata aattatataa aaatcaacat
     1081 attaactcaa tctaacaagc cgttttttgc caaacagccg tttttttata tacaatcaac
     1141 aagatatttt cgactgatac agcataacat cgcacggcgg cacgatgcct cctgcgcgga
     1201 aacaccgata tggattcttt tttcaaaccg gcagtttggg cggttttgtg gctgatgttt
     1261 gccgtccgcc ccgcccttgc cgacgagttg accaacctgc tcagcagccg cgagcagatt
     1321 ctcagacagt ttgccgaaga cgaacagccc gttttaccca tcaaccgagc ccccgcccgg
     1381 cgggcgggca atgccgacga actcatcggc agcgcgatgg ggcttaacga acagcccgtt
     1441 ttacccgtca accgagtccc cgcccggcgg gcgggcaatg ccgacgaact catcggcaac
     1501 gcgatggggc ttaacgaaca gcccgtttta cccgtcaacc gagcccccgc ccggcgggcg
     1561 ggcaatgccg acgaactcat cggcaacgcg atgggacttt tgggtattgc ctaccgctac
     1621 ggcggcacat cggtttctac cggttttgac tgcagcggct tcatgcagca catcttcaaa
     1681 cgcgccatgg gcatcaacct gccgcgcacg tcggcagaac aggcacggat gggtacgccg
     1741 gttgcccgaa gcgaattgca gcccggagat atggtgtttt tccgcacgct cggcggcagc
     1801 cgcatttccc atgtcggact ttatatcggc aacaaccgct tcatccacgc gccgcgcacg
     1861 gggaaaaata tcgaaatcac cagcctgagc cacaaatatt ggagcggcaa atacgcgttc
     1921 gcccgccggg tcaagaaaaa cgacccgtcc cgctttctga actgatttcc caaggaatac
     1981 gcaatgagta tgcccgaaat gcccaaatgg tacgacgatg acggacagat cgtgtcctgt
     2041 accgaaaagg tcaaagtgat gtccgaaaat atggccgagc tgtatcagac ggcacaagac
     2101 gcgtttgaag acgcgctgct gatgggttgc ggcgaacgtc aattgcgcga ttacctgctc
     2161 gcgctgattg aaggtttgga aaatccctac cgcaaagtct gaacacgccc cggttgctgc
     2221 ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc ttcggctttt cagacggcat
     2281 atttgacgtt atgattaaac agttaacaag atttatcaca acgccgtcaa aagacagaca
     2341 cacaacatga acatcatccg cgcgctcctc atcatcctcg gctgcctcgc cgccggcgaa
     2401 accgccgttt tcctagcagg catcaaactg cccggcagca tcgtcggcat gggcgtgctg
     2461 tttgcgcttt tgcaggcggg ttgggtcaaa acgtcttggc tacaacagct taccgacgcg
     2521 ctgatggcga atctgacgct gtttctcgtg ccgccctgcg tggcggtcat cagctatttg
     2581 gatttgattg ccgacgattg gttttcgata ctggtttccg cctccgccag cactttgtgc
     2641 gtactgctgg ttacaggcaa ggttcaccgc tggatacgga gcattatctg atgaacgaaa
     2701 tcctcaggca gcccagcgtt ctgcttttcc tcacgcttgc cgtgtacgcg cttgcgatta
     2761 tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt actcgtcagc actatcgtgc
     2821 tgattgccta cctgaaaatc ctcgacatcg attatgcggt gtaccacaac gccgcgcaat
     2881 tcattgattt ttggctgaaa cccgccgtcg tcgtgcttgc cgtgccgctc taccaaaacc
     2941 gccgtaaaat cttcaaccag tggctgcccg tcatcgtttc acagcttgcg ggcagcgtta
     3001 cgggcattgt tacggggatg tattttgcca aatggctggg cgcggaacgc gaagtcgtcc
     3061 tctcgctcgc gtccaaatct gttaccaacc ccatcgccat tgaaatcacc cgctccatcg
     3121 gcggcattcc cgccattacc gccgccaccg tcatcattgc cggtctggtc ggacagattg
     3181 ccggttacaa aatgctgaag aacacagtcg tcatgccctc gtccgtgggt atgtcgctcg
     3241 gcacggcttc gcacgcgatg gggattgccg cctcgctcga acgcagccgc cgtatggcgg
     3301 catacgcggg actggggctg acgttcaacg gcgtactgac cgcgctgatt gcgccgctgc
     3361 tcatccccgt tttgggattt tgaacccgtt tcagacggca tttcagccca tgctgtctga
     3421 acgccgacac actcgcaagg agaaccgtta tggctgtcaa cctgaccgaa aaaaccgccg
     3481 aacaactgcc cgacatcgac ggcattgccc tctacaccgc ccaagcaggc gtgaagaagc
     3541 ccgggcatac cgacctgaca ctgattgccg tagccgccgg cagcaccgtc ggtgcagtct
     3601 tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc caaatcgcac cttttcgacg
     3661 aagacggcgt gcgcgccctc atcatcaaca cgggcaacgc caacgcgggt acgggcgcac
     3721 agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc ccggcaaatc ggctgcaaac
     3781 cgaaccaggt gctgcccttc tccaccggcg tgattctcga accgctgccc gcagacaaaa
     3841 tcatcgccgc gctgcccaaa atgcagcctg ccttctggaa cgaagcggca cgcgccatca
     3901 tgaccaccga caccgtgccc aaagccgcct cgcgcgaagg caaggtcggc gacaaacaca
     3961 ccgtccgcgc cacgggcatc gccaaaggct cgggcatgat tcatcccaat atggcgacca
     4021 tgctcagttt catcgccacc gatgccaaag tttcccaacc cgtcctccaa ctgatgacgc
     4081 aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga cggcgacacc agcaccaacg
     4141 acagcttcgt catcatcgcc accggcaaaa acagccaaag cgaaatcgac aacatcgccg
     4201 acccgcgtta cgcccaactc aaagaattgt tgtgcagcct cgcgctcgaa ctcgcccaag
     4261 ccatcgtccg cgacggcgaa ggtgcgacca agttcatcac cgtccgcgtc gaaaacgcca
     4321 aaacccgcga cgaagcccgc caagccgcct acgccgtggc acgttcgccg ctggtcaaaa
     4381 ccgccttttt cgcctccgac cccaacctcg gcagactgct cgccgccatc ggttatgccg
     4441 gcgttgccga cctcgatacc gacctcgtgg aaatgtatct cgacgatatt ttggttgccg
     4501 aacacggcgg acgcgccgca agctacaccg aagcacaagg gcaggcggtg atgtcgaagg
     4561 ccgaaatcac cgtccgcatc aagctgcatc gcggacaagc cgccgccacc gtctatacct
     4621 gcgacctgtc gcacggatac gtttccatca acgccgacta ccgttcctga cccgacacgg
     4681 cttcagacgg catacataaa atgccgtctg aaccgccgga caacatacca tgacctccac
     4741 attcccccgc cgcctcgccc gcaaaatccg ccaaacccgc cgcctgtcgc gcaaaagcat
     4801 cgcctttctg ttccttttgg caggttcggc actcgtcgcc ctgaccgcgc tgttttttgc
     4861 ccatcttgcc gattttgcgc tggaactgaa cgccaaactg gttcaacaat acccgtggtt
     4921 cgcgtgggtc gcgcttcctt tgggtttacc gcttattgcg tggctcacac gcaaattcgc
     4981 ccccttcacc gccggcagcg gcatcccgca ggtcatcgcc tcactgtcgc tgccctacgg
     5041 cgcacagaaa acgcggctga tccgcctcgg gcagacactg ctgaagattc cgctgacctt
     5101 tttgggtatg ctgttcggcg cgtccatcgg acgcgaaggt ccgtccgtgc aggtcggcgc
     5161 ggcagtgatg ggcgcgtggg gcgcgtggtg caagaaacac ggcttggcat tcaaagggat
     5221 gcaggaaaac gatttgatgg cggcgggcgc ggcgggcggt ttggcagccg cgttcaacgc
     5281 gccgctggcg ggcgtgattt tcgccattga ggaactcggg cgcggcatca tgttgcgctg
     5341 ggaacggcag attctgctcg gcgtactcgc cgccggtttc atacaggtcg ccattcaggg
     5401 caacaacccg tatttttccg gcttcaacgg cggcgtattg gaacatatct ttctgtgggt
     5461 cgcactgtcc ggcctggttt gcggcgcggc gggcgggctg ttcggacgtt tgctctatcg
     5521 cggtgcggcg gcgtttgcac cgcgcaagat acgcggcttc atccgcaacc gtccgctgct
     5581 gctggcggca ctgatggggc tgctgctcgc cctgctcggc acgttctacc aaggcaaaac
     5641 ctacggcacc ggctaccacg aagccgccca agccctgcac ggcatctacg aagccccctt
     5701 cggactcacc gccgccaaat ggctcgccac cgtattcagc tattgggcag gcattccggg
     5761 cggcattttc actccctcgc tgaccatagg cgcggttttg ggcgagcata tcgccgccat
     5821 cgccgacata tcgcagggtg caaacatcat cgtcctcatc tgcatggcgg catttctggc
     5881 gggcgcgaca caatccccga ttacttccgc cgtcgtcgtc atggaaatga cgggcggaca
     5941 aagcctgctg ttttggatgc tgattgcctg cattttcgcc tcgcaggttt cgcgccagtt
     6001 ttcgccgcgt ccgttctacc acgcatcggg aatgcgcttc cgccagcgcg tgcttcaaga
     6061 aaccgccgcc caaaccggca atgcgcccgc aagaccgcaa acagcaaaac gggaatgccg
     6121 tctgaaaatt aaaacgcccc cgatcaaacg ccggcagccg ccttgattcg aataccgttc
     6181 cgccgccgct tgaaatttca gcaacaatgc cgtctgaacg acagaatgcg gttttcagac
     6241 ggcatttccc catcccgata ttgcctaaac aaaaccgaag cgtttgctat aattgcactt
     6301 tttaccgcac acgcacgccc atgtttcccg atttctccca aaccctctcc aaagaccgcc
     6361 acttcctgca atccgccttc aaaaatccca acaaatacgg cggtttgtcc aaagtcgaag
     6421 aaaaataccg aaaatcgcac gaaatctttt tgaggcgttt ggccgcattg cccaaacccg
     6481 aattcgacaa caccctgccc gttcacgaaa aactcgaaga aatcaaaaaa accgttgccg
     6541 aacatcaggt aacgattatt tgcggcgaaa ccggttcggg caaaaccacg cagctgccca
     6601 agatttgttt agaactcggg cgtggggcgg cggggctgat cgggcatacc cagccgcgcc
     6661 gtttggccgc gcgctccgtg gcagagcgga ttgccgaaga gctgaaatcc gaaatcggca
     6721 gcgcggtcgg ctataaagta cgcttcaccg accacacctc gcgcgatgcc tgcgtcaagc
     6781 tgatgaccga cggcatcctg ctggcggaaa cgcagaccga ccgttatctc gccgcctacg
     6841 acacgattat cattgacgaa gcgcacgagc gcagcctgaa catcgacttc ctcttgggct
     6901 atttgaaaca actgctgccg cgccgccccg atttgaaagt catcatcacc tcggcaacca
     6961 tagacgcaga acgcttctcc caacacttca acggcgcgcc cgtgctggaa gtgagcggac
     7021 gcacctatcc cgtcgaaatc ctctaccgac cgctgaccgg caaagacgaa gacgatgcag
     7081 aagtcgagct gaccgacgcg attgtcgatg cagccgacga attggcgcga cacggcgaag
     7141 gcgatatctt ggtattcctg ccgggcgagc gcgaaatccg cgaagccgcc gaagccctgc
     7201 gcaaatccac gctgcgccgc aacgacgaaa tcctgcccct gttcgcacgc ctgtcgcacg
     7261 ccgagcagca caaaatcttc cacccctcag gcgcgaaacg ccgcatcgtg ctggcaacca
     7321 acgtcgccga aacctcgctt accgtgccgg gcatcaaata cgtcatcgac accggcctcg
     7381 cacgcgtcaa acgctattcc gcacgggcga aagtggagca gcttcatatc gaaaaaatct
     7441 cccaagccgc cgcccgccaa cgctccggcc gctgcggacg cgtctccgca ggcgtatgta
     7501 tccgattgtt ttcagaagaa gatttcaaca accgccccga atttaccgac cccgaaatcg
     7561 cccgcagcaa cctcgccgcc gtcatcctgc gcatggcatc gctgaaactc ggcgacgtag
     7621 cggcattccc atttttagaa atgcccgatt cgcggtatat taatgatggg tttcaggtgt
     7681 tgctggaatt aggggcagtg gaggccgcct gaaaacaagt aggatataca cttctacctg
     7741 acaaagacca acaaacgata aaactacggc acatctagat gcccaaccta caacattgca
     7801 cacggtaaaa acatgaaaca cttactcatc gattttgaaa acgtccagcc gcaaaactta
     7861 gacaaattac taaccgaaaa tacccatatt tggctattta taggtgtatt acacaaaatg
     7921 ttacctatta gtctggtgca atccctacta cgtttcggcg aacgtgtcca tcttgtccag
     7981 ttacaaaaaa cggggaaaaa cgcattggat ttttacctgt cctattacct cggacaaatt
     8041 accgccacag accccaatgc ccaagtcggc atactctcgc gtgatggagg atacgatgtt
     8101 ctggtcgaac atattttgaa aaaccaccag gcgaagggta tcgtgcgcct agccaatata
     8161 gatgaagtac aacatcagaa aattgctacc gaaccgccgt cagcattgct ggaaaacact
     8221 cctcagcctg aaaccaccct caaaccacag caaccattaa cttcctattt ccaagcagcc
     8281 ctaactgcac tgcgccgccc cgacgctttc cgcccctgcc gcctgcataa cctgcgacaa
     8341 aatctgcgta agcatatttt gagtgatttg tttaaagaaa aaaccgatga agaatgcgaa
     8401 ataaccactg ctaacgttat caataaactc aaagcacaaa acttcatcag cattgatgaa
     8461 caggaaaccg tttcctacca tctcagtgat aatgatttgt tacaaagaat ccaacgccat
     8521 attttaagcc aacgtcccaa aacctacgct gattttcaag ccgtcgtgca aaaccgagca
     8581 gatgcacttc acttaacagt cggtaccaac gacattcaat cctttgcgcg acatttgcgc
     8641 gaccaaaacc tgatccgcca aaacaatggg aaaattgaat atgcaccgtt tactgaacct
     8701 aaaccacagc caacgcccaa gcagcctaaa aaaaccgcat gggaacctga tgaaattatt
     8761 tggaaaaaag tgattgccgc gttatcgtta aagaaccgtc ctaataaaac caaaacttta
     8821 cgcaatacaa tccaggcact cacaaaatcc aatgcacaag aaactgacaa actgctacaa
     8881 catttacaag atacccaagt cctacgtatt gacggtacta aaattgtgta tatcaagtag
     8941 gatgggcatt tatgcctgac ctacaaaacc aaaaacaacc taccaccctt aatcaactcc
     9001 acaaaccctt ttcagacaac ctcgtttttt gaaaaacaat ctgtaaacag atagctgctg
     9061 aagaataccg ttgccgagcc ccaaaacccg tcctgcaact tttattgtga acttcccatt
     9121 atgaaaaaat cccttttcgt tctctttctg tattcgtccc tacttactgc cagcgaaatt
     9181 gcttatcgct ttgtattcgg aattgaaacc ttaccggctg caaaaatggc ggaaacgttt
     9241 gcgctgacat ttatgattgc tgcgctgtat ctgtttgcgc gttataaggc ttcgcggctg
     9301 ctgattgcgg tgtttttcgc gttcagcatt attgccaaca atgtacatta tgcggtttat
     9361 caaagttgga tgacgggcat caattattgg ctgatgctga aagagattac cgaagtcggc
     9421 agtgcgggcg cgtcgatgtt ggataagttg tggctgcctg cgttgtgggg cgtgttggaa
     9481 gtcatgttgt tttgcagcct tgccaagttc cgccgtaaga cgcatttttc tgccgatata
     9541 ctgtttgcct tcctaatgct gatgattttc gtgcgttcgt tcgacacgaa acaagagcac
     9601 ggtatttcgc ccaaaccgac atacagccgc atcaaagcca attatttcag cttcggttat
     9661 tttgtcggac gcgtgttgcc gtatcagttg tttgatttaa gcaggattcc cgcctttaag
     9721 cagcctgctc caagcaaaat cgggcagggc agtgttcaaa atatcgtcct gattatgggc
     9781 gaaagcgaaa gcgcggcgca tttgaagctg tttggctacg gacgcgaaac ttcgccgttt
     9841 ttaacccggc tgtcgcaagc cgattttaag ccgattgtga aacaaagtta ttccgcaggc
     9901 tttatgactg cagtgtccct gcccagtttt ttcaatgcga taccgcacgc caacggcttg
     9961 gaacaaatca gcggcggcg
//
LOCUS       DAXIHS010000072         9900 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXIHS010000072 DAXIHS010000000
VERSION     DAXIHS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.072"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..708
                     /gene="lpt3"
                     /locus_tag="ACFOF2_001606"
     CDS             <1..708
                     /gene="lpt3"
                     /locus_tag="ACFOF2_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC7107887.1"
                     /translation="MFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            774..4007
                     /locus_tag="ACFOF2_001607"
     CDS             774..4007
                     /locus_tag="ACFOF2_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC7107888.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTAKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPNFYTADAVSDDLHPTNPQQTAPSPVGEGWGEGKTVAAQTNFSATAA
                     NPLPNPLPQEREQSAAASAVSNDLHPANPQQTAPSPVGEGWGEGKTVAAQTNFSATAA
                     NPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTLSDD
                     PKPKKQPAPPKGRLKPLPLADIRTFQAWIKTAERDNLRLLFLSRDDLMQHAAAHITEE
                     QFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEWLVPGMLREKIQ
                     LLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDN
                     VTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTIEAAEQAHRQGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEK
                     AFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPS
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLVKQ
                     GLPISDGLAGFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLNK"
     gene            4066..5673
                     /locus_tag="ACFOF2_001608"
     CDS             4066..5673
                     /locus_tag="ACFOF2_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC7107889.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNAIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPLKFKPNILF
                     HKGDKKSDVNERLIHSLTQSISDIRKQLIDTQKQVEKKYEKYQQYLQSPFVAQADIDV
                     AISGILDQIDKLNLRIKDCERLESLCR"
     gene            5664..7124
                     /locus_tag="ACFOF2_001609"
     CDS             5664..7124
                     /locus_tag="ACFOF2_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFC7107890.1"
                     /translation="MSLNRKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQAKLALLQKNPFILHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPNKQNLPLKLLTGKWQQQLDYAKSQWYLKQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(7478..7951)
                     /locus_tag="ACFOF2_001610"
                     /pseudo
     CDS             complement(7478..7951)
                     /locus_tag="ACFOF2_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8183..8395
                     /locus_tag="ACFOF2_001611"
     CDS             8183..8395
                     /locus_tag="ACFOF2_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFC7107891.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            8579..8847
                     /locus_tag="ACFOF2_001612"
                     /pseudo
     CDS             8579..8847
                     /locus_tag="ACFOF2_001612"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(8937..9386)
                     /locus_tag="ACFOF2_001613"
     CDS             complement(8937..9386)
                     /locus_tag="ACFOF2_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC7107892.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(9474..>9900)
                     /locus_tag="ACFOF2_001614"
     CDS             complement(9474..>9900)
                     /locus_tag="ACFOF2_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC7107893.1"
                     /translation="HPYGVRSGLRRNGLKLWDIHFRMTRFIVARCGLLFATLKGKTMK
                     KMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDD
                     LTKVKGIGPAVLAKLKDQASVGAPAPKGPAKPVLPADKK"
BASE COUNT         2929 a         2499 c         2142 g         2330 t
ORIGIN      
        1 atgttccgcc tcgccaaaga gcagggctat gagacgtatt tttacagcgc gcaggcggaa
       61 aacgagatgg cgattttgaa cttaatcggt aagaaatgga tagaccatct gattcagccg
      121 acgcagcttg gctacggcaa cggcgacaat atgcccgatg agaagctgct gccgctgttc
      181 gacaaaatca atttgcagca gggcaggcat tttatcgtgt tgcaccaacg cggttcgcac
      241 gccccatacg gcgcattgtt gcagcctcaa gataaagtat tcggcgaagc cgatattgtg
      301 gataagtacg acaacaccat ccacaaaacc gaccaaatga ttcaaacagt attcgagcag
      361 ctgcaaaagc agcctgacgg caactggctg tttgcctata cctccgatca tggccagtat
      421 gttcgccaag atatctacaa tcaaggcacg gtgcagcccg acagctatct cgtgccgcta
      481 gtgttgtaca gcccggataa ggccgtgcaa caggctgcca accaggcttt tgcaccttgc
      541 gagattgcct tccatcagca gctttcaacg ttcctgattc acacgttggg ctacgatatg
      601 ccggtttcag gttgtcgcga aggctcggta acgggcaacc tgattacggg tgatgcaggc
      661 agcttgagca ttcgcgatgg caaggcggaa tatgtttatc cgcaataagc gacgtgaaaa
      721 ccaataaaaa caaatttaga tgatgtcggg gaagatgccc gaccgacaag actatgcaaa
      781 atatgaaaaa ccaagtacgc ggatcaggca tggatgcccg atccaatccg gccaatgttt
      841 cagacggcct gcaaaacagt tcgggtcata tcggtaccaa cacgcgctac cgcctgacca
      901 aactcggcga acaaatggcg cgcctgccca tcgacccgaa aatcgcgcgc attttgctgg
      961 cggcgaagaa acacgactgc atggcggaaa tattggtgat agcgtccgcg ctgtcgattc
     1021 aagacccgcg cgagcggccg ttggaagcgc gcgatgccgc cgccaaggca cacgagcgtt
     1081 ttaccgacaa gcaatccgac ttccttgcct atctgaacat ttgggacagc ttccagcgcg
     1141 agcgcgacaa aggtttgtcc aacaagcagt tggtgcaatg gtgccgtcaa tatttcctgt
     1201 cgcacctgag gatgcgcgag tggcgcgagc tgcaccacca gcttgcccaa accgcgattg
     1261 aaatgggttt gaccgccaag gaaaccgctt tcagacgacc tcccgaaatc aggcagctca
     1321 cgtcgtctga aaatgcgggt gaccaagact tatccgccaa gctcaaacaa aaacaactgg
     1381 ataaaaagca acaccgcgcc caaatccgcg ccgccaaaga agcgggctac gaacaaatcc
     1441 accgcgccct gctcactggc cttatcgcca acgtcggcat gaaatcgccc gacggcaacg
     1501 actacaccgg cgcgcgcggc agccgcttcc accttttccc cgcctccgcc ctgttcaaag
     1561 ccaaacccaa atgggtgatg gcggcagaac tcgttgaaac gacaaaactt tatgcgcgcg
     1621 acgtcgccgt tatccagccc gaatggattg agcaggaagc cccgcacctc gtccgctacc
     1681 actatttcga gccgcactgg gaacaaaaac gcggcgaagt cgtcgccagc gaacgcgtga
     1741 cgctttacgg tctgaccgta ttgccgcgcc gccccgtgtc ttacggcaaa gttgcccccg
     1801 aagaagcgcg cgaaatcttt atccgcagcg cgttggtggc gcaggaatgc gatttgaaag
     1861 cggatttttt tgtccacaac aaaaagctga ttaaagaaat taccgaactc gaacacaaat
     1921 cgcgcaagca agacgtgctg gtcgatgacg aagccctgtt tgcgttttat aacgaacgac
     1981 tgcccaattt ttatacggcg gacgcggttt cagacgacct gcatcctaca aatccgcagc
     2041 aaactgcccc ctcccccgtg ggggagggct ggggagaggg caaaacagtt gccgcacaaa
     2101 ccaacttttc cgcaaccgca gcaaaccctc tccctaaccc tctcccacag gagagggagc
     2161 agagtgccgc agcttcagcg gtttcaaacg acctgcatcc tgcaaatccc caacaaaccg
     2221 ccccctcccc cgtgggggag ggctggggag agggcaaaac agttgccgca caaaccaact
     2281 tttccgcaac cgcagcaaac cctctcccgc aggagaggga acagagtgcc tcagcttcaa
     2341 cgttttcaga cgacctgcgt cctgcaaatc tgcagcaaac cgccccctcc cccgtggggg
     2401 agggctgggg agagggcaaa acagttgcca cccaaaccaa cttttccgca acctcaacac
     2461 tttcagacga ccccaaaccc aaaaagcagc ctgcaccccc aaaaggccgt ctgaaacccc
     2521 tccccctcgc cgacatccgt accttccaag cctggatcaa aaccgccgaa cgcgacaatc
     2581 tgcgcctgct gttcctcagc cgcgacgatt tgatgcaaca cgccgccgca cacattaccg
     2641 aagagcaatt ccccaaattc tggcaaaccg cagacggcaa gttcaaactt tcctaccgct
     2701 tcgagccgca ccatccgctc gacggcgtta ccctcaccct accgctgacc gtcctcaacc
     2761 gcctgcacgc gccgtcgctc gaatggctgg tgcccggtat gttgcgcgaa aaaatccagc
     2821 tgctgattaa agctctgccg aagcaaatcc gccgcatttg cgtgcccgta cccgatttca
     2881 tcacccaatt tttaagccaa aaccctgatc gcaacgcccc catcctgccc caacttgccc
     2941 aagccatcgc caaaaccgca ggcgacatcc gcatactcga gcaaatcaac caagacgaat
     3001 gggccgcgtt caggctgccc gaacactgct atttcaacct ccgcattatc gacgacggcg
     3061 gacaagagct tgccggcggc cgcaaactgc acgaattgca acaacaactc ggtcaagccg
     3121 ccgccgttac cttccgcgac aacacccaag aattcgagcg cgacaacgtg accgcatggg
     3181 acatcggcac cctgcccgaa tccatcaaat tcgcacgcgg caaacaacag ctcaccggct
     3241 atctcggtct gcaaaaagaa aaagacggcc gcatcgccct gcgcctgtgt gacaccatcg
     3301 aagccgccga gcaggcacac cgtcaaggcg tcatcgaatt gatgaagctg caattaaaag
     3361 agcaggtaaa agacctgaac aaaggcatcc aaggcttcac ccaagccgcc atgctgctca
     3421 aacacatcaa cgccgacacc ctgcgcgacg acctcaccca agccgtctgc gaccgcgcct
     3481 ttatcggcga agacgagctg ccgcgcaacg aaaaagcctt caaagaacaa atcaaacgcg
     3541 cccgcagccg cctgcccgcc gtcaaagaag ctctcagtcg ttacctgcag gaaaccgccg
     3601 ccgcctatgc cgaactgaac ggcaaactcg gcaaacaccc gctcacccat ctcctaagac
     3661 tacgcctgca aaccctgctt gcccccagct tcgccacccg caccccgtgg gcacaatggc
     3721 cgcgcctccc catctatctc aaagccatga ccctgcgcct cgaaaaatac agcagcaacc
     3781 ccgcccgcga cgcagcccgc gaagccgata tccaagagct ggaacaaatg tggcaggaaa
     3841 aaaccgatag cttggtgaaa caaggattgc ccatttcaga cggccttgca gggtttaaat
     3901 ggatgattga agaattgagg gtgtcgctgt ttgcgcagga attgaagaca ccgtatccgg
     3961 tgtcggtgaa gcggctgatg aaggagtggg agaggttgaa taagtagtga tattatccaa
     4021 tacatttaca ggaaaatttc taacttacat aaaaggtaca actcaatgaa aagcgaaaat
     4081 aattatgaac gtattgttca aaattctaaa aaattccaac actctcagaa gaatgagatg
     4141 cagaaacgca taacagattt gctgcaacaa ctcacaaatg gcattcatga acgcgaccaa
     4201 attatggcgg tttccctact tggtgcgatt gccggacata atactttcct atttggaccg
     4261 ccgggaaccg cgaaaagtct gatttcccgc cgtttggcat gtgcatttga aaatccgcaa
     4321 tactttgaat acttgatgaa ccgtttcagc acacctgaag aagtattcgg acctgtatct
     4381 attaaggcat tgaaagaaga ccgatatctc cgaaaaatcg aaggctattt gccaactgcc
     4441 gattttgcat ttttggatga aatttggaaa tccagtcctg caattttgaa taacctgctg
     4501 accattatca acgagcatat ttttaaaaac ggcgatgaga ggtttaatgt gccacttaaa
     4561 gccctgattg cggcttccaa cgaaattcca cctgaaaatc aaggtttaga ggcactgtat
     4621 gaccgcttta ttatccgtct gcttgtgccg ccgattgaac aagaagagaa cttcaaccgt
     4681 ttgctggata gcaaaccaag cagcgacaaa cctgaaatca gtgaagattt acggattagt
     4741 tatcatgagc ttgccaaatg gcgtgaacaa ctccacaaag tcaggctaag tacagataca
     4801 ttactgatta ttaagtatat cagagaagaa ttagcagccc agtttgataa gttaggcgtt
     4861 tatgtatccg accgacgttg gcaacgtgca gccgtattat taaaggcttc tgctttttgc
     4921 aatggacgca aagaaaccaa tcatagcgat gtgatcttgc tgaaatattg tctgtggaca
     4981 acacctgaaa atcgagagtc agtggaacaa attgtgatga atgccattaa atcctgtggg
     5041 tttgatacag gtattgatct ggctgaatta gatagagaaa aagagagttt ggataaggaa
     5101 ataaataagg aactttatca ttcacaagat gtttatgaga cggttgaaat gagtggaaaa
     5161 aaatattttc cagtacaggc tgttttcaaa aacgtacata atagtcatgc taataaagaa
     5221 cttaagattt atgttccata tagcaaattt cgtagtaaag aaaaatttta tccaatagat
     5281 agtacaggaa atgaaattca agaaattgaa tgtgaatttg atgggcaagg ttgctgtaaa
     5341 ttggaattga aagtgcgggg tggtcactgg aatgagcaat ataagccatt aaaatttaaa
     5401 cccaatatct tatttcacaa aggagataaa aaatcagatg ttaacgaacg tctaattcat
     5461 tcactaaccc aatctatatc tgatattcgc aaacaattga ttgatacaca gaaacaagtt
     5521 gagaaaaaat acgaaaaata ccagcagtac ctgcaaagcc cctttgttgc ccaagcagat
     5581 attgatgttg ctatatcagg tattctagac caaatcgata aactcaattt gcggattaaa
     5641 gactgcgaac gtttggagtc tttatgtcgt taaacagaaa attatccgaa caaataagat
     5701 ggattaaagc ttctctacaa atagattatg cagatttatt taaacagcat ggttcgttaa
     5761 atggacagat tcagcaacgt ttatcagatt ggcaatatca ggccaaactg gctttgttgc
     5821 agaaaaaccc atttatatta catgaagaaa agctaaaaaa acaccaacaa acagaaattt
     5881 ttaatcaaca gtctgttgat gattatcggt atttttgcaa attagcaggc gtgccatttg
     5941 aaaaagaatt ctggcagcag gaattaacac aaagcaaaaa ttcaaaaccc aataaacaga
     6001 atttgccttt gaaattgcta acaggaaaat ggcagcagca attagactat gccaaatctc
     6061 aatggtattt aaagcaactt aatcaattac gacaggaatt tatagataag ttaaaacagg
     6121 aactcgacac tatccgacag cttgcagaac agttagagca atttggtttt gaagcagggc
     6181 tgtggtttga taacagtatc ggcaatttaa gccagcaaaa tattgaagaa atgaaacgct
     6241 ggctgaatta cttgacccaa gataagaatg cccaacagat tgctgaatta ctaggcaaaa
     6301 tgcgccaaat cgaacaatct gaaaaaatcg aacaggtaaa acagactgtt tacacaaaaa
     6361 gcccacaaat tgatgtgaat tcgcgtgaag aaattatcgg attacgttta ggtaaagatt
     6421 tagagtatgt attgccgtct gaattggctt taatggcaga tgaagaaaca tcgactttat
     6481 ttgatttgaa gtttttggaa tccaaattaa tgtgttttga attgcaagga atgacctatt
     6541 gcgatgcccc aactgaaatt atagttgaac aaaaaagcca agaggatgaa aagctcggac
     6601 caatgatttt atgtgtagat accagtggtt caatggttgg tctacctgaa aatattgcca
     6661 aagcaatggc tttattttta ggagcaaaag ctaaaagcga aaaccgttct tgttttgtta
     6721 tcaatttttc cactggaata gaaacttttg aattaacaag taaaacaggg atttccaatt
     6781 tgattgcttt tttacgtcaa tcctttcatg gtggtacaga tgctgcccca gctttacgtc
     6841 atgcgctaaa aatgatggag caagaaatct atcaaaaggc agacttatta atgatttccg
     6901 attttgtgat gaacagctta cctgatgatt tgttggcatc aatcgaaata caacgagaaa
     6961 ccggtaatca gtttaattct ttggttattg gtgatgcttt tatgagtaaa cgcctaaaaa
     7021 cacattttga tcgtgagtgg atatacaatc ctaatgttca gacaattcaa gaattagtac
     7081 agttcaagaa agatattttc aataaacaag ttgtcaactc ataacactat cctactcata
     7141 tgtatttttg gaagatacca ccctatacaa cttggaatcc atacgcagaa tatgtgtgga
     7201 ctttctaaac tactcccctt tccaccctaa aaaaggtcgt ctgaaaccca ttttcagacg
     7261 acctttttcc acttcgcaaa acaagaattc ccacccctct ccccaaacgt cgcccgaacc
     7321 agtaaactcc ccatatttca acaacttctt ggaagccaac catgccccgt atccaccctc
     7381 ctcacctgtt cccgccccat atcgccgaac gcggcctttt gtattttcag cagggcaagg
     7441 ttcgcgatgt ccgaaaaact tccgccgggc gttatcgggc ctggcgccgt ttgctgccgg
     7501 cggtctgaat aatggtggcg tcaacgacgg cagcggatgc tttctctact tttaggtttt
     7561 tttcggtcag ttggcggtta atcagtttga gcaattcgga cagggtgtcg tcttgcgcca
     7621 gccagttgcg gtaacggcat aaggtgctgt aatcggggat gctcagttcg tcaaagcggc
     7681 aaaacaggtt gaagtcgatg cgggtgatga ggctgtgctc gagttcggga tcggagaggc
     7741 tgtgccattg tccgagcaga acggctttga acatggacaa cagcgggtag gcgggacgac
     7801 cgcggtggtc tcggaggtaa cgggtttttt gacggttcag gtattgttcg atcggttgcc
     7861 aatcaatcac ctggtccaac ttcaatagtg ggaaacggtc gatgtgtttg gcaatcatgg
     7921 cttgggcggt ttgctgaaag aaggtgctca tgagaaatct cctaaatgtc ttggtgggaa
     7981 tttaggggat tttggggagt tttgcaaagg tctcagtctt tttaaaccac agcccttccc
     8041 aactaaacca aaaggtcgtc tgaaccctat tttcagacga ccttttgcca ctttgtaaaa
     8101 caaatcttcc caccatcctc tccccaaaca tcgcccgaac cagtaaactt ctcatatttc
     8161 aacaacttct tggaagcaaa ccatgcccca tatccacccg ctccacctgt tcccgcccca
     8221 tatcgccgaa cgcggactgc tctattttca gcagggcaaa gttggtgctg tccaaaaaac
     8281 ttccaccggg cgttatcggg cggaggtatg cggtttggaa aactattggg tatggctgaa
     8341 gctggatagt gatttgtata ttaaagacga aagctgcgat tgcccttatg tctaaaggtg
     8401 caaacatacc ttaaattact atattacata ggcaaaatac aagcctataa cgaattggaa
     8461 acaaaatgcc gtctgaaagc attataaaat ctgttcacct tttcagatga gtaatgtaca
     8521 cccctataca atttttgcta ctttacccca taaatccacg gctaaagata tccttattat
     8581 gtcctatgat ttatcgaaac gacttgtaat cggcttagca tcaagtgccc tattcgactt
     8641 atccgaatcg gataatatat ttagaatgga aggggcagaa acctataggc aatatcagag
     8701 agaaaaacaa aaccatcccc taaaaaaggc gttgtctttc catttattaa aaaacttctg
     8761 tcaatcaatg aaataaaccc aaacgaccca acgattgggt ttattctttt atccagaaac
     8821 aatccagata cagattacga gtcataacta taggcttaat attacacgat tctcattcca
     8881 tcaaggcgga aaaccgcaca aatactgaaa cactatcgat cgatttgtaa acaagcctac
     8941 ttaagtaact tgcagtcctt atcatttcct ttaaaataat ccagcccgtc actacacgaa
     9001 ctggcggact tcttgcaaat aaaggttact agattttcat tcatcttaat aataaaagga
     9061 tttttatctt tatctatggc taccgccttc aacatgaatt tactgtctaa ggccccgcgc
     9121 gcgattccat tcaaacggat acaaaagcct tctgcctctt taatcggcaa acttggccat
     9181 ttggtagatg tttgtttaaa tctcccattc tgcagataaa acttttccat aaaatgtgca
     9241 ttttctaaca aggctgcccg cactgcattt atctttgctt tctcaacata attgcgatag
     9301 ctcggataaa caattaaagc aagtacagac aatatcaaga ccactgatat taattcaacc
     9361 agcgtaaacc cccgattatc agtcattact ttacttccaa taagaacaga ttattcaaca
     9421 tatttctttg aacagactta ctatcccatt cagcagtatg catatttccc actctatttt
     9481 ttatccgcgg gcagcactgg tttagctggg ccttttggtg cgggtgcgcc gaccgaagcc
     9541 tgatccttca gcttcgccag caccgcaggg ccgatgccct ttaccttggt caaatcgtcc
     9601 acagacttga acgcaccgtt ttgcgcacgg tattccgcaa tggccttcgc cttcgcaggg
     9661 cctatgcccg gcagcgcctc caactcctgc tgcgaagccg cattgatgtt taccgccgca
     9721 agggagaagg cgcaggagaa cagcatacag aacagcacga acattttctt catggttttt
     9781 cctttaaggg ttgcaaacaa taaaccgcat cttgcgacga taaaacgggt cattctaaaa
     9841 tgaatatccc aaagtttcaa gccgttcctc cgcaaacccg accggacgcc gtacggatgc
//
LOCUS       DAXIHS010000073         9693 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXIHS010000073 DAXIHS010000000
VERSION     DAXIHS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.073"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..335
                     /locus_tag="ACFOF2_001615"
     CDS             <1..335
                     /locus_tag="ACFOF2_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC7107894.1"
                     /translation="GVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNA
                     PIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQ
                     AHLEKVFG"
     gene            complement(543..1928)
                     /gene="pntB"
                     /locus_tag="ACFOF2_001616"
     CDS             complement(543..1928)
                     /gene="pntB"
                     /locus_tag="ACFOF2_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC7107895.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1994..2377)
                     /locus_tag="ACFOF2_001617"
     CDS             complement(1994..2377)
                     /locus_tag="ACFOF2_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC7107896.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2381..3922)
                     /locus_tag="ACFOF2_001618"
     CDS             complement(2381..3922)
                     /locus_tag="ACFOF2_001618"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC7107897.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKDVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            4212..5045
                     /gene="serB"
                     /locus_tag="ACFOF2_001619"
     CDS             4212..5045
                     /gene="serB"
                     /locus_tag="ACFOF2_001619"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC7107898.1"
                     /translation="MPHALVLQFPSAETLPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKVQAAADACINFGGLERVRGLF
                     G"
     gene            5109..6332
                     /locus_tag="ACFOF2_001620"
     CDS             5109..6332
                     /locus_tag="ACFOF2_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC7107899.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6493..8073
                     /gene="purH"
                     /locus_tag="ACFOF2_001621"
     CDS             6493..8073
                     /gene="purH"
                     /locus_tag="ACFOF2_001621"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC7107900.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRITARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
     gene            8686..9642
                     /locus_tag="ACFOF2_001622"
     CDS             8686..9642
                     /locus_tag="ACFOF2_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC7107901.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHK
                     VALVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFF
                     AQMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNRLHQVHETQVGSVK
                     QLIAHFDRLIDELDKQIDNHTHTHFDGKDQVAEQIKGIGSITTATLMAMLPELGRLSH
                     KRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFHQRPL
                     SEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT         2226 a         2628 c         2712 g         2127 t
ORIGIN      
        1 acggagtatt aaccgctgga cacggcaaac tctaccaaag cgacggcaga cacagcgtca
       61 atccgactga gggctacggc acaggcggct tgttgcaagg caaaaaacat atgctttcac
      121 tgacttggaa tgcgccgatt gaggcgttta cccgcgaagg cgatttcttt gagggcaaag
      181 gcgttgatgt tttgtatatg cacttccaca aagccaacga gtttttgggt atgacccgcc
      241 tgccgacatt cttatgtaac gatgtggtta aaaatccgca agtggaaaaa tacttggcag
      301 attatcaggc acacttggaa aaagtgttcg gctaattaaa aatccatctt caacacggag
      361 atggattttg tttgtttcgt tgattttgtg tcagtttcag atgtaggtgc ttattcggac
      421 gggccgtctg aaaatgtttg ccccaatgca aaaaaatcac tgcaaacctt cataaacggg
      481 gtttgcagtg attttttcaa atcaaacaga ttgaaaacct gcgccgaatt gttcagacgg
      541 cattattttt tcagttcgga cagaatgtca tctacggttt tcttcgcatc tccgaaacac
      601 atcacgctgt tttcgttgaa gaacagtggg ttttgtacac ctgcgtagcc ggtattcatc
      661 gagcgtttga agacgacgac ttcttttgcc ttccacactt ccaacacggg catacccgca
      721 atcgggctgt tcgggtcggt ttgggcggcg gggttgacgg tgtcgttcgc accgatgacc
      781 aagaccacat cggtttcggg gaagtcgtcg ttgatttcgt ccatttccaa aacgatgtcg
      841 taggggactt tggcttcggc gagcagtacg ttcatatgac cgggcaggcg gccggcgacg
      901 gggtggatgc cgaagcgtac ttcggtgccg tttttacgta aaagctcggt gatttcggca
      961 acggggtatt gcgcttgtgc gactgccata ccgtagcccg gggtaatgat gacattgttt
     1021 gcgcctttca gcatttcggc aatatcggca gctttgactt ctcggtattc ccctatctct
     1081 tggctgccgg aagataatgt gccgctgtcg ctgccgaaac cgccggcaat taccgagaca
     1141 aacgagcggt tcatggcttt gcacataatg taggacagaa tcgcgccgct tgagccgacc
     1201 agcgcgccgg taacgatgag caggtcgttg gagagcatga agcctgccgc tgcggccgcc
     1261 cagccggagt aggagttgag catggacacg accacgggca tatctgcgcc gccgatggag
     1321 gcaaccaagt gccagccgaa tgcgagggca atcagggtca taatcagcag gatgaagccg
     1381 ctaccgttaa tgccgacgaa tacgagcagc aacacaaacg atacggcgag cgccagtgcg
     1441 ttgagcttgt gtttggcggg cagttgcagc gggctgctgc tgattttgcc gttgagtttg
     1501 ccgaatgcga ccagcgagcc ggtaaaggtt accgcgccga tgaaaatgcc caaatagact
     1561 tcgaccagat ggatggtgtg catatcgtgc gaaacgttgc ccggctcgat atagctgttg
     1621 aagccgacca aaaccgccgc taggccgacg aagctgtgca gcagggcaat cagttcgggc
     1681 atttcggtca tttccacctt tttggctttg tagatgccga ttgccgcgcc gatgagcatg
     1741 gcgatgatga tccagcccag tccgtgggta ttgtcggaaa aaacagttac aaaaagggcg
     1801 accgccatac cggcgatacc ggaatagcag ccctgtttgg cggtttcctg tttggacagc
     1861 cccgccagtg agaagatgaa taaaattgcg gcaacgatat acgccgctgt tacgagtcct
     1921 gaagacatgg aaattctccg attttcgatg atttgttttt aatgccgtct gaactgttca
     1981 gacggcatct gtttcaagca gccgcgacaa acagcccgac ggtgcaggca aatccgcccg
     2041 cccacatcag cgagcgcaat gctgctttgt ctgcgatgta gcaccagata aaggcgaggc
     2101 ggaacaggat gaacaggccg gcaagcgtgt tgacggttgc ttgtccggca ttgccggttg
     2161 cgtgtgccgt caaaacggcg gcggcaaacg gtgcaaaggc ttcaaaaccg ttttgctgcg
     2221 cggcgtgggc acgggcggct gtgccttgcg tgcgcgccag aaaatcgcgc ggattgtggt
     2281 tgtctttaaa ccggaatccg cccgcttttt tggcatatgc cgcacaaaaa agcggcaata
     2341 agcaggcaat caggatgcac caataggcaa aagtcatgct ttatcctttc ctaaacatat
     2401 tcaacatgcg gcgcgttacg aagaagccgc cgaagatatt gatgctggca atcaggatgg
     2461 cgatgaaggc aagcagggta acgaaaccgt tgccttggct gatttgcagc agcgcgccga
     2521 cgacgatgat gccggagatg gcgttggtta ccgacatcag cggtgtgtgc agcgagtggc
     2581 tgacgttcca aacgacatag tagccgatga cgcaggcgag gacgaagacg ataaagtggt
     2641 tcaggaatgc tgcgggtgcg accgcgccga cccacagtac caacacggcg gcgatgacgg
     2701 cgggcgcgag ttttttccac aggggaacgg gtttcggttc gggcttggcg gcaggcgcgg
     2761 ctttttcaga cggcgtttgc tgcggttggg cggaaacttg aatcggcgga ggcgggaagg
     2821 tgatttcgcc gtcgcgggta acggtcatgt tgcggataat cacgtcttca aaatccagcg
     2881 tgatttcgcc gtctttgttc gggcttaaca acttggtcag gttgaccagg ttggtggcgt
     2941 aaagctggga agactgtccg gcaaggcggt ttgccatgtc ggtgtagccg atgattttca
     3001 cgccgttgcc ggttacggac aattcgcccg gtcgggtgag ttcgcagttg ccgcccgtcg
     3061 ccgccgccaa atcgacgatg acggagccgg atttcatgct ttccaccatt tctttggtaa
     3121 tcagcttggg cgcgggtttg cccggaatgg cggcggtggt gatgatgatg tccacgtctt
     3181 tcgcctgctc ggcaaagagc ttcatctcgg cggcgataaa ttcgtcgctc atcactttgg
     3241 catagccgtc gccgctgccg cccgattctt gcgggaagtc gagtttcaaa aacttgccgc
     3301 ccatcgattc gatttgttcc gccacttcca agcgggtatc gaacgcgcgc accactgcgc
     3361 cgagcgagtt tgccgtaccg atcgccgcca aacctgccac acctgcgcca atcaccaaaa
     3421 cctgcgcggg cggcactttg ccggcggcgg taatttgacc ggtgaagaaa cggccgaagg
     3481 cgttggcggc ttcaattacg gcgcggtagc cgctgatgtt tgccatcgaa gacaaagcgt
     3541 ccaaagcctg cgcgcgcgaa atgcggggca ccatatccat cgccagcgcg ttcactttct
     3601 tggcgcgcaa ggcttcgacc aaagcctcgt tttggcgcgg ccacaggaag ctgacgatgg
     3661 tttgaccttc gttcaaaagc ggcagttcct gttcggacgg cgcgttgacc ttataaatca
     3721 aagggcagac ccaaaccgcc gctttgtcgg caacggttgc gcctgctgtt tggtaagcgg
     3781 catcgtccaa acttgccgcc aaacctgcac cgctttcgac aacggtttca aagcccagtt
     3841 tgcccagcag ggcgacggtg gcgggcgtgc aggcgacgcg ggtttcgccg gataatgact
     3901 cgcgtgggat accgattttc atctctgaat cctttttcgg gtagtttata tgtatcgtgg
     3961 gttaaattta aatcggggca gggcggagca acgccgtacc ggtttaaaac cgattcactt
     4021 caaatgttaa tatattttag ataatcccct tataacgaat tttcatcagg ctggcaatag
     4081 ttgcggcatt ttcccgtgtt gtccgacaca tactgccgtt tgcgtttgaa gtcattttcc
     4141 gattgcagtc ccgcccgccg taacattctt tgccgtttca ctatataatc cgcatttttt
     4201 gagccgccgt tatgccgcac gccctcgtcc tccaatttcc ctccgccgaa accctgcctt
     4261 ccgacttccc ctcacgcctg cccgaacctg attatgctga tgaaaagcgt atgcgtttta
     4321 tcgttgaaga agggttttct ttaagcgaaa aagacgcggc gttacttggc agccgtcaaa
     4381 tcgaccacgc cgtgttgtcg gatatggatt ttggcgaact cggtttgatt gtcagcgata
     4441 tggattcgac gctgattacc atcgaatgcg tcgatgaaat tgcggcaggc gtgggtttaa
     4501 aagacaaggt agcggaaatt accgagcgtt cgatgcgcgg agagttggat ttcgagcagt
     4561 cgttgcgcag ccgcgttgcg ctgttggcgg gattggacga acagattttg gcggacgttt
     4621 atgaaaacgt tttgaagctc tcgcccggtg cggaattctt gttggacgaa tgcaaaaggc
     4681 acgatgtgaa attcctgctg gtgtccggcg ggttcacatt ttttaccgaa aggctgcaac
     4741 aacgactcgg cttcgaatac caacacgcca atgttttgga aattgaaaac ggcaggttga
     4801 ccggtcgtct gaaaggcaga atcatcgacg cgcaggcaaa ggcggattta ttgcgcgaat
     4861 accgcagccg cctcggattg cagccgcatc aggtgttggc gatgggcgac ggtgcgaacg
     4921 atattccgat gctcaaagaa gcgggcatag gcgtggctta ccgtgccaaa ccgaaagtgc
     4981 aggcagccgc cgatgcctgt atcaacttcg gcggtttgga gcgtgtacgc ggcctgttcg
     5041 gataggcgga taggaaacgg atgccgtctg aaaggttttc agacggcatt tgaacggcag
     5101 gaacgacagt gggacgcaga aaactttggt ttgccctggc agcagcgggc gttatcggcg
     5161 gtttggtcgg cattgtgctg acggaactga tgcacttcat acaacatacg gcatacggtt
     5221 atggcacgga cggcgtgtcc atctcgttcc gcgaaggtgt ggcacaggct tccggtatgc
     5281 ggcgcgttgc cgtgctgacg ctgtgcggcg cggtcgcagg cagcggctgg tggttgctga
     5341 aacgtttcgg caagccgcaa atcgaaatca aagccgcctt gaaacagccg ttgcaggggc
     5401 tgccgtttct gacgacggtt ttccatgttc tgctgcaaat cataacggtc ggactcggtt
     5461 caccgctcgg acgggaagtc gccccgcgtg aaatgactgc cgcgttcgct tttgccggcg
     5521 gcaggcggtt gggtttggat gaagacgaaa tgcggctgct gattgcttgc gcttcgggtg
     5581 cgggtttggc ggccgtgtat aacgtgccgc tcgcctccac actttttatc ctcgaagcca
     5641 tgctgggcgt gtggacgcag caagccgtcg ccgccgcatt gctgacttcc gtcatcgcca
     5701 ccgccgtcgc gcgcatcggc ttaggcgacg tgcagcaata tcatcccgcc aaccttgccg
     5761 taaacacttc attactttgg ttttccgccg tcatcggccc gatactgggc gcaaccgccg
     5821 tctttttcca gcgcaccgcc caaaaattcc ccttcatcaa gcgcgacaac atcaaaatca
     5881 ttcccttggc agtctgcatg tttgcgctca tcggcgtgat tgccgtttgg tttccagaaa
     5941 ttttgggcaa cggcaaagca ggcaaccaac tgacctttgg cggtttgacc ggttggcaac
     6001 acagccttga gctgaccgcc gtcaaatggc tggttgtctt gatggcgttg gcggcaggcg
     6061 catacggcgg actgattacg ccgtctatga tgctcggcag caccatttct tttgcagccg
     6121 ccgccgcgtg gaacagcgtt ttccccgaaa tgccgtctgg aagcgcggca gtcgtcggag
     6181 cggcggtgtt tctcggcgta tcgctcaaaa tgccgctgac cgccattgtg ttcgtattgg
     6241 aactgaccta cgcgccgctg tccctgctgc tgcctttgtg catcggcatg gcgggggcat
     6301 tgggaacatc gggaaaaatg ggcttcaaat aaatgccgac aaatgccgtc tgaaaggttt
     6361 tcagacggca tttctttgta aattgtcagc aatatctgca aaaacctact gccaaaaaac
     6421 tagaaatagc ggataatacc gccccgaaaa agttcccctt tccccatcca actgtcagaa
     6481 aaggaagccc caatgtccgt catcaaacgc gccctgatca gcctgtccga caaggcaggc
     6541 gcggtcgaat ttgcccaaac cctgaccaag ctcggtgtcg aaatcctctc caccggcggc
     6601 acggcgaaac tgcttgccga tgcgggcgtt cccgtgattg aagtcgccga ctataccggc
     6661 ttccccgaaa tgctcgacgg ccgcgtgaaa acgctgcatc cgaaaatcca cggcggtatt
     6721 ttgggtcggc gcgatttggg cgaacacgtc gccaagatgg aagaacacgg catcggcaat
     6781 atcgaccttg tgtgcgtcaa cctttatccc ttcgccgcca ccatcgccaa accaaactgc
     6841 acactggaag acgcgattga aaacatcgac atcggcggcc cgaccatggt gcgttctgcc
     6901 gcaaaaaact ggaaacacgt cgccatcgtt accgacaccg ccgacttccc cgccatcgcc
     6961 gccgaactcg aagccaacaa cggcgcgttg agcgacaaaa cccgcttcaa cctgtcgcgc
     7021 aaagcgttca gccacaccgc ccaatacgac ggcatgattt ccaactacct gacctcgctt
     7081 tcagacgaca tcttgagcgg cacgcccgaa atcggtgaat tcccgagcca gttcaaccaa
     7141 agctggatta aagtgcaaga catgcgctac ggcgaaaacc cgcatcagcg cgccgcgttc
     7201 taccgcgata ttgaccccgc cgcaggcagc ctttccgcct acaaacaact gcaaggcaaa
     7261 gaattgtctt acaacaacat cgccgatgcc gatgccgcat gggaagccgt caaatccttt
     7321 gacgcgccag cctgcgtgat cgtcaaacac gccaatccgt gcggcgtcgc cgttgcagcc
     7381 gatacattga ccgcctacaa actcgcctac gtcaccgaca ccaccagcgc gttcggcggc
     7441 atcatcgcct tcaaccgcga agtggacggc gaaaccgtca aacagattac cgacaaccag
     7501 tttatggaag tcttgatggc gccgaagttt accgccgaag ccctcgaaat cgccgccgcc
     7561 aagaaaaacg tgcgcgtatt ggaagtgccg cttgaggcag gcgcaaaccg cttcgaactc
     7621 aaacgcgtcg gcggcggact gttggtacaa acgcccgaca tctgccgcat cagccgcgcc
     7681 gatttgaaag tcgtctccaa acgccaaccg accgagcagg aatggaacga tttgctgttc
     7741 gtctggaacg tcgcaaaata cgtcaaatcc aacgccatcg tgttcggcaa aggcggacaa
     7801 acctacggca tcggtgcagg ccaaatgagc cgcgtggaca gcacccgcat caccgcccgc
     7861 aaagcgcagg acgccaattt cgacctcaac ggcgcgtgtg ccgcctccga tgccttcttc
     7921 cccttccgcg acggcgtgga tgtgattgcc gaacagggca ttaaagccat catccatccg
     7981 gcaggttcga tgcgcgatca agaagtcttc gacgcggcag acgaacacgg cattgcgatg
     8041 gtggtaaccg gcatccgcca cttccgccat taaggcagat gaacaaggca atgccgtctg
     8101 aagggctttc agacggcatt ttgcgctatt ttgtgaaggt agggatgacg gttcgggtat
     8161 tcctgacagg gtggattttc aaggtgttgt atagggtgta ggaggattcg taaaaggtgg
     8221 gatgcagggt gtgcttcagc ccgctgcatc aaaaattttt ggagaaccgg cgggagtcgg
     8281 cggttttggt ttcggcgggg acggtggaaa tgggtaacat tgacggaatc gacggaagcg
     8341 gtgggctgaa gcccaccctt gtatattgga atcactgtat catagcaaca aaccgcccgg
     8401 ccgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag cggcagggca
     8461 acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg caggtttatg
     8521 atgagtaccc aataccatta cgcaggtata gtgaattaaa tctaaaccag tacagcgttg
     8581 gttcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag tgagtcggtt
     8641 ccgtactatc cgtactgtct gcggctcgcc gccttgtcct gatttttgtt aattcactat
     8701 atcgacatcg ccaaacgaaa cttcgtcatc gccgtttcgt ctttgtctaa aaccaaaacc
     8761 gaaaccaaca accccaaagg tatcgcccat actatcgaat accttaaaaa acacaaggtc
     8821 gccctcgtcg tgacggaaag tactggcggt ctcgaaatcc ccgccgccaa agccatccgc
     8881 cgagcaggca tagccgtgat tatcgccaac ccgcgtcaga cgcatcagtt tgcccaatcg
     8941 cagccgctga ccaaaaccga cgccaaagat gccaaaatgc ccgccttctt cgcacagatg
     9001 acggcacaga aagaagattc gcaaaccatg ccctaccaac cgcccaccga agcggaagaa
     9061 gtgttggaag ccttggttaa ccgccgcaac caaccggcgg atatgcggac tgccgagaaa
     9121 aaccgtctgc atcaggttca cgaaacgcaa gtcggaagcg tcaaacaact gattgcccat
     9181 tttgaccggc tgattgacga attggacaaa caaatcgaca accacaccca cacgcatttt
     9241 gacggcaaag accaagtggc ggagcaaatc aaaggcatcg gttcgataac gacggctacg
     9301 ctgatggcga tgttgcccga attggggcgg ctgtcgcaca aacggatagc gggtttggcc
     9361 ggcattgccc cgcacccgag ggagggcggg gaaaccaaat tcaaaagccg ctgctttggc
     9421 ggaaggtctg cggtgcgtaa agcactgtat atggctaccg tggcagcgac acgttttgaa
     9481 ccgcttattc gggatttcca ccaacgcccg ctgtccgagg gtaagccgta taaggttgcc
     9541 gttacggcat gtatgcgcaa actgctgacg atatcgaatg cccggatgcg tgattatttt
     9601 gccgaaaacg ataccgccga aaacggtatc cggacggctt gatttgagtt ttggtatttt
     9661 tgcccgacgg ggtgaaaaat acagttgctt ttt
//
LOCUS       DAXIHS010000074         9548 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXIHS010000074 DAXIHS010000000
VERSION     DAXIHS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.074"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            107..217
                     /locus_tag="ACFOF2_001623"
                     /pseudo
     CDS             107..217
                     /locus_tag="ACFOF2_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            468..1925
                     /locus_tag="ACFOF2_001624"
     CDS             468..1925
                     /locus_tag="ACFOF2_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC7107902.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2397..2756)
                     /gene="rnpB"
                     /locus_tag="ACFOF2_001625"
     ncRNA           complement(2397..2756)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFOF2_001625"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2835..3938)
                     /gene="prfB"
                     /locus_tag="ACFOF2_001626"
     CDS             complement(2835..3938)
                     /gene="prfB"
                     /locus_tag="ACFOF2_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC7107903.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(4041..4934)
                     /locus_tag="ACFOF2_001627"
     CDS             complement(4041..4934)
                     /locus_tag="ACFOF2_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC7107904.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5276..5725
                     /locus_tag="ACFOF2_001628"
     CDS             5276..5725
                     /locus_tag="ACFOF2_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC7107905.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            5799..6107
                     /locus_tag="ACFOF2_001629"
     CDS             5799..6107
                     /locus_tag="ACFOF2_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107906.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            6223..7065
                     /locus_tag="ACFOF2_001630"
     CDS             6223..7065
                     /locus_tag="ACFOF2_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC7107907.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            7055..7801
                     /locus_tag="ACFOF2_001631"
     CDS             7055..7801
                     /locus_tag="ACFOF2_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC7107908.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLST"
     gene            7823..8410
                     /locus_tag="ACFOF2_001632"
     CDS             7823..8410
                     /locus_tag="ACFOF2_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC7107909.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8416..8595
                     /locus_tag="ACFOF2_001633"
     CDS             8416..8595
                     /locus_tag="ACFOF2_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC7107910.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8827..9267
                     /locus_tag="ACFOF2_001634"
     CDS             8827..9267
                     /locus_tag="ACFOF2_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC7107911.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         2400 a         2584 c         2169 g         2395 t
ORIGIN      
        1 agaatcgttc tctttgagct aaggcgaggc aacgccgtac cggtttttgt taatccgcta
       61 taatgcgccc ttcggcgtgg cggatatata aggaagtgat tttccatcta agtatagtgg
      121 attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
      181 cgaggcaacg ccgtactggt ttaaatttaa tccactataa aaaaccgccc tatcggataa
      241 gcccttaaca gaaaaggctt tacccgcgcc gtatcggaac acatcctcta aaatacaatc
      301 cgttgaattg aaaaaaatat aaaaacatcc gcccgcgaaa aacggcagcg cgtcgtttga
      361 caaagaatga aaatatcggt taaaaaccga ttttcataca aaaaacaccg ctgccgtccg
      421 catccgtttc agacggtatt gagagaaaat cttttaggag aacctttatg tcccggcatc
      481 ccgcccccac cggagaaaaa acattcttcg gccacccctt ccagctttcc accctcttcc
      541 atatcgaatt gtgggaacgt ttttcatttt acggaatgca gggcatcctg ctgatttacc
      601 tctactacac cgccgacaaa ggcggcttgg gcatagacaa aaccctcgcc ggcggcattg
      661 tcggcgcata cagcggcagc gtgtacctgt ccaccatttt gggggcgtgg tttgccgacc
      721 gagtatgggg tgcggaaaaa accctcttcc tctcgggcat cgtcgtgatg ctcggacaca
      781 tcgtccttgc cgccgccccg ggcctgtacg gccttttaat cgggctgata ttcatcgcat
      841 tgggcagcgg cggcgtgaaa tctacggcca gttctatggt gggcgcatta tacgaacagg
      901 acgaaatgcg gccgctgcgc gatgcgggat tttccatttt ctacatcgcc atcaatatcg
      961 gcggcttcct aggcccgctg ctgaccggcc tgctgcagga aaacatcggt ttccattatg
     1021 gtttcggcgc ggcggcggtc ggtatggcat tcggcttgtg gcgttattcc ttgggacgta
     1081 aaaacctgcc ccatcccatc gtcccccatc cgctttcaaa aggacagggc aaaactgcgg
     1141 ccgccgtcgg cattgctctc atcgccgcac ttgcaaccgc catcaaaacc gggcttgtca
     1201 acctcgacaa tttctccggc atcctcttat ctaccgtcat ccttgccgtc attgcctatt
     1261 ttgcccgcct gctgactaat ccccgcgtca gttccgacaa caaacggcac atcatcgcct
     1321 acatcccgct tttcctgacc atctgtatgt tttgggccgt ctggtttcag atttacaccg
     1381 tagcaaccgt ctatttcgac gaaaccgtca accgcaccat cggttcgttt accgtgcccg
     1441 tcgcctggaa agattccatg caaagcctgt gggtcatcct gttttccgga ctgatggcgg
     1501 caatgtggac aaaaatgggg cgcaaacagc ccaaaacccc gctgaaattc gctatggcgg
     1561 tatttgttac cggcgcgtcg tttttgggat tcgtcccctt tatttccgcc ggcacgccga
     1621 tgcctattgc ggttttcgca ctgattgtcc ttgccatcac gataggcgaa ctgatgattt
     1681 ccccgattgc gctgtccatc tccaccaaaa tcgcaccgcc tttattcaaa acccaaatgg
     1741 tcgcccttaa tttccttgcc ttttcattag gcttcacttt gggcggcgta ttgtttgaaa
     1801 aatgctatca ggcgggcgac gaaatcggct tctatcggct gctgttctac atcggcgcag
     1861 ccacaggctt cctgctgctc ctgctcgtcc ccaaattgaa caaaatgctc gaaggcacag
     1921 actaagtccc gccccgatgc cgtctgaacc cttcagacgg catttttccg cataatgaaa
     1981 ccaaaccgtt tccacccgac aggacaggct ccccccaacc ggaaggcagc ctgccgattg
     2041 tcatttaaat atctgactga ttccgtctgt ttgcaaggga aatagccgtt tatttccgct
     2101 tgggcggaaa ccggttctat tgagtaaaag gcattttatc cgactaaatt agtgctgcat
     2161 caacggaata tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
     2221 caaacagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
     2281 gctaaggcga ggcaacgccg taccggtttt tgttaatcca ctataaaaac acaacctaaa
     2341 taaaaatgcc gtctgaacca tatttcaggt ttcagacggc atttgcgtgt cggatgcaca
     2401 ccggacgggc ggtaagccgg gttctgtctc ggacagtcat tcctctaggc ataccgttac
     2461 cggtatgctc aagcaaccta cccgaacgct cggcgggcag cgtcattgcg ttctgtttgg
     2521 tcttgctccg aatggggttt ggcctgccgc atattgttac caaatgcgcg gtgcgccctt
     2581 accgcacctt ttcacccttg cctgtgctgc caaagcagcc atcggcggtt ttgctttctg
     2641 ttccactttc cgtcgcgtta ccgcgcccgg ccgttaaccg gcattctacc ctgcggagcc
     2701 cggactttcc tccccgtatg ccttacgcga tacgcggcga ctgtctgccc gtcccgtgtg
     2761 cggcgcggat tataacacga aacgcaaaaa tgccgtctga aacggtacag gtttcagacg
     2821 gcatacagcc taaactacac gccctgtttc aggctggctt cgataaagcc gtccaaatcg
     2881 ccatccaata cggctttggt gttgccgact tcgtagcctg tacgcaagtc tttgatacgt
     2941 gaggaatcca aaacatacga acggatttgg ctgccccaac ctacatcgga tttaccttct
     3001 tccaacgcct gtttctcttc attgcgtttg cgcatttcca attcatacag tttggacttc
     3061 aacatttcca tcgcggcggc tttgttggcg tgttgcgaac ggtcgttttg acattgcacc
     3121 acaatccccg tcggctcgtg ggtaatgcgc acggcggagt cggttttatt gatgtgctga
     3181 ccgcccgcac ccgatgcgcg ataggtgtcg atgcgcaaat cggcggggtt gatttcgatt
     3241 tcgatggaat cgtcgatttc gggatagacg aacacggagg caaacgaggt atggcgtttg
     3301 ttgttcgagt caaacggaga gtaacgcacc aagcggtgaa cacctgtttc cgtgcgcagc
     3361 aagccgtagg catattcgcc ttccactcgg atagtcgcgc ggttgatgcc cgcgatttcg
     3421 ccgtcgtctt cttcgaggat ttcgattttg aagcctttgc gctcggcgta gcggctgtac
     3481 atgcggaaca gcatacccgc ccagtcttcc gcttccgtac cgcctgcgcc tgcggtaatg
     3541 tcgataaagc agttgttcgg atcggcgggc tggttgaaca tccgtttgaa ctccaaatcc
     3601 gccatctgtt tttccagccc cgccacatct tcctgcacgg cggcaaaacc ttcttcgtcg
     3661 ttttcttcga cggtcatttc aatcagcatg cggttgtctt cgatgcccga agcgatgttg
     3721 tcgagcgtca acacgatgcc ttcgaggatt ttgcgctctt tgccgatttc ttgggcgcgt
     3781 ttcgggtcgt tccaaagttc ggggtcttcg gaaagaccga caacttcttc caatcggtct
     3841 ttcttaccct gataatccat ataaactcgg atgtcttcgc tgcgcttttc caaatcattc
     3901 agggtattgt tgagctggtt gattacttcg gcttccatga ttcttttgtt ctttcaaaat
     3961 tttaggggcg tattgtacgg gattcgggta tttttttcta tggataaagc cttctggaaa
     4021 cacgttcgga cggcatagcg tcaataacgg tatgccgcca gtttgcgttt gatttcaggc
     4081 aatgcggcac gtgctgcctc ctcacccaac cggatggcgc gttttttctg atcgaatccg
     4141 ccgactgcac ccaaatccaa aacctgcggt ttgataacca catccgcctg ccccaactca
     4201 ttttgcaacg cggaaacgct cattacgttc agcgtctgat cgagataaga gaagaagcct
     4261 tggctgatgt ttttgcccgg acgggcggaa atatcgacgg caatcacgaa attcgccccc
     4321 tgccgccggg cggcactgac gggcacgggc tgcgacagac cgccgtcaac atatgtatgc
     4381 ctgccgatga taacgggttg gaacacattg ggaatggcgg cggaagcgcg cacagcctgc
     4441 ccggcattcc cttgattgaa agcgacggcc ttgccggttt caaaatcagt agcaacagcg
     4501 gcaaatttga tgggaaactg ctgaatctgc ctgccgccga cttttcggtt gatgtaattt
     4561 tgcagctttt cgcctttgat aaaaccactg gtggacaagg ttaaatcgac taaatcggtt
     4621 ttgcctaaaa tttcggcttc caattcgagg cggtcgggcg acatacccga tgcaaaaagg
     4681 ctgccgacta tcgaacctgc cgatgtgccg gtaaccacct tcacaggaat accgttttct
     4741 ttcaaaacct taataatacc tacatgggca aatcctttag atgcgccgcc accgagtgcc
     4801 aaaccgacca ctgcggcggg tttggcggtt tgcaccggct tgcgggcagc attatttccc
     4861 gccgtgccgc aggcggcaag caacgcggcg gcggcgattg ccaaaagcgg tctgattttt
     4921 gaaaacgtta ccatattttc cattccttta tatatcgcac cccgtcaaaa agagggattg
     4981 cttttcttaa caaccccctt tgacagccaa gcaaatgggg gctttgttaa gtcatcatca
     5041 aaattaatat ttcttttttt cctttacgga aattatattt gaaggcatac tatccaaggc
     5101 gggaattatc tcacaacacc gccgttatcc aaatatcccg cctttttcct ttcctttcca
     5161 tcaaaatact ttctttttat attcattaac ttgttaaatc attggctgcc gggtgtcagt
     5221 ttttccgaca aaatccgtct aatggggtat caacagaacc aaaacaggaa cacttatgaa
     5281 aatcggaaca acttggcaga cggcatccgc tatgctggtt ttgcgtctgt ttgccgcata
     5341 tgaatttttg gaatcgggtt tgcaaaaatg gaacggggag aattggtttt ccgaaatcaa
     5401 cgatcagttt ccattcccgt tcaacttgct gccggacgcg ttaaactgga atctcgccat
     5461 gtatgcggag cttttactgc ccgtactgtt gcttttgggt ttggcaacgc gtctgtcggc
     5521 attagggctg atggtcgtta ccgccgtcgc ttgggctgcg gttcacgccg gttcgggtta
     5581 caatgtctgc gacaacggtt ataaaatggc tttaatttat atcgtggtat taatcccgct
     5641 gcttttccag ggtgcgggcg gatggtcgct ggatacgctg ctgaaaaaac ggttttgccc
     5701 ccaatgccgt ctgaagcaag attgattcag tcgtggaatc tgactttaaa cattccaacc
     5761 ttatctcgtt aacttgatat tctgaaaagg aaatagaaat gaacaaaaac attgctgccg
     5821 cactcgccgg tgctttatcc ctgtctttgg ccgccggtgc agttgctgcc aacaaaccgg
     5881 caagcaacgc aacaggcgtt cataaatccg cccatggctc ttgcggcgcg tccaaatctg
     5941 ccgaaggttc gtgcggcgcg gctggttcta aagcaggcga aggcaaatgc ggcgagggca
     6001 aatgcggtgc gaccgtaaaa aaaacccaca aacacaccaa agcatctaaa gccaaggcca
     6061 aatctgccga aggcaaatgc ggcgaaggca aatgcggttc taaataatcc ccccccttca
     6121 aaccaagccg cgtttttcag taaaatgcgg cttttttaac ggcaaacaaa gattttttaa
     6181 caagcacatc attcttttgt gccatccgaa ccgggtaaaa atatgattca acacgcaggc
     6241 ttgggctacc gccgcgactt ggcggaagac tttctctcgc tttccgaaaa cagcccgata
     6301 tgctttatcg aagccgcacc ggaaaactgg ctgaaaatgg gcggctgggc gcgcaaacag
     6361 tttgaccgtg tggcggaacg gctgccgctg gcgttgcacg gattgtctat gtcgctgggc
     6421 gggcaagcac cgctggatac tgatttgata gacggcatca aagaaatgat gcgccgttac
     6481 gattgcacgt ttttctccga ccatttgagc tactgccacg acggcggtca tctttacgat
     6541 ttgttgccgc tgcccttcac cgaggaaatg gtgcatcata cggcgcggcg tatccgtgaa
     6601 gtgcaagacc gtttgggctg ccgcatcgcc gtggaaaaca cgtcctacta tctgcattcc
     6661 ccgcttgccg agatgaacga ggtcgagttc ctcaacgccg tcgcacgtga ggccgattgc
     6721 ggcattcatc tggatgtgaa caatatctac gtcaacgccg tcaatcacgg cctgttgtcg
     6781 ccggaggctt ttttgaaaaa cgttgacgca gatcgcgtgt gctatatcca tattgccgga
     6841 catgacgtgg aaacgccgga attgttgatt gatacacatg gcgcggcagt tttgccgact
     6901 gtttgggact tgctcgaact tgcctatgcc aagctgccga cgattccgcc caccctgttg
     6961 gaacgcgatt ttaatttccc gccttttgcc gaactcgaag ccgaagtcgc caaaatcgcc
     7021 gattatcaaa cgcgtgccgg aaaggaatac cgccgtgcag cctgaaacct ccgcccaata
     7081 ccagcaccgt ttcgcccaag ccatacgcgg gggcgaagcc gcagacggtc tgccgcaaga
     7141 ccgactgaac gtctatatcc gcctgatacg caacaatatc cacagcttta tcgaccgctg
     7201 ctataccgaa acgcggcaat actttgacag cgaagaatgg agccgtctga aagaaggttt
     7261 cgtccgcgac gcgcacgccc aaacgcccta ttttcaagaa atccccggcg agttcctaca
     7321 atattgccaa agcctgccgc tttcagacgg cattttggca ctgatggatt ttgaatatac
     7381 ccaattgctg gcagaagttg ctcaaattcc ggatattccc gacattcatt attcaaatga
     7441 cagcaaatac acaccctccc ctgcggcctt tatccggcaa tatcgatatg atgttaccga
     7501 tgatttgcat gaagcggaaa cagccttgtt aatatggcga aacgccgaag atgatgtgat
     7561 gtaccaaaca ttggacggct tcgatatgat gctgctagaa ataatgggtt cctccgcgct
     7621 ttcgtttgac accctcgccc aaacccttgt cgaatttatg cctgaggacg ataattggaa
     7681 aaatattttg cttgggaaat ggtcaggctg gactgaacaa aggattatca tcccctcctt
     7741 gtccgccata tccgaaaata tggaaggcaa ttccccgggc caaaaccatc tatccacata
     7801 aaattacctt gttcccgata ctatgccgct acccgacctg accgatgccg aattaataga
     7861 gtcgcgtaaa ctgcttctgc attttgcgcg gcttcagttg cccgaccacc ctgatttggc
     7921 tgaagattta gtgcaggaaa cattgctgtc cgcatacagc gcaggcgaca gttttcaagg
     7981 cagggcactt gtcaacagct ggctttttgc catattgaaa aacaaaatta ttgacgcatt
     8041 acgtcaaatc ggaaggcaga ggaaagtctt taccacactg gatgacgagc tactggatga
     8101 agcatttgaa agccattttt cccaaaacgg gcattggacg ccggaagggc agccgcaaca
     8161 ttggaacact ccggaaaaat cattaaacaa caacgaattc caaaaaattc tgcaaagctg
     8221 cctatacaac ttgcctgaaa acaccgcacg ggtatttacc ctgaaggaaa tactcggttt
     8281 ttcatccgac gaaatacaac aaatgtgcgg tatcagcacg tccaactacc acaccattat
     8341 gcaccgcgcc cgagaatcat tgcgccaatg cctgcaaatc aaatggttca accaagaaaa
     8401 cccgaagtaa acgttatgaa aaaatgccgc gatatcgccc tgcttctttc caaacatcag
     8461 gaccgggaaa ccaccccggg cgagaagatt tccatataca cacacctgct gttctgccca
     8521 cattgccgag aatataaaag acagcttcaa accatcaaaa tatcactggc aaaaacaacc
     8581 agaacttcaa aataaatgcc gtctgaaaag gcttcagacg gcataagctg acggaaaaaa
     8641 atcaaaccga tttactgtta tcggcagttc atccataata cacgcttcaa aagcagcata
     8701 ttcctccata cggaatatat aaatacgcaa aacacgaagg ctgcatcaat ttgctttatt
     8761 tgccttattt cacagacggc gctacccctc ccgcccaacc cgttctttct gaatgagcag
     8821 atttcaatga ttaaggaaac cctaatgcgc ccaatcttcc tatctttcgt tttactccct
     8881 attttgataa ccgcctgcag cacaccggac aagtctgccc gatgggaaaa tatcggcaca
     8941 atctcaaacg gcaatattca tacatatatc aataaagaca gcgtgagaaa aaacggaaat
     9001 ctgatgattt tccaagataa aaaagttgtt accaatctaa aacaagaacg ttttgccaac
     9061 acccccgcat acaagactgc cattgccgag tgggaaatcc actgcaacaa caaaacatac
     9121 cgcttaagtt cgctacagtt gtttgataca aaaaacacgg aaatttccac acaaaactac
     9181 acagcctctt ccctccgccc gatgagcatc ctgtccggga cattaaccga aaaacaatat
     9241 gaaaccgtat gcggaaaaaa actctgattg caacttatac acaaacttac ccacaaacct
     9301 tatcataaaa atgccgtctg aaatactgaa atatcagcat ttcggacggc attttgccat
     9361 ttctccgaaa agttatcctc gaagttatcc acattatttt tttaaaccag cttccatccg
     9421 aaatataaac ctgctataca atttcattat caaaacgtaa attgttccat tgatacacaa
     9481 aactgcttac ccccataatt ttgataaagc atttcttaca ttcccggctc cgtcccgtaa
     9541 tcaacaca
//
LOCUS       DAXIHS010000075         9510 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXIHS010000075 DAXIHS010000000
VERSION     DAXIHS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.075"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFOF2_001635"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFOF2_001635"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC7107912.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHTQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3486)
                     /locus_tag="ACFOF2_001636"
     CDS             complement(3151..3486)
                     /locus_tag="ACFOF2_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107913.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            complement(3500..3973)
                     /locus_tag="ACFOF2_001637"
     CDS             complement(3500..3973)
                     /locus_tag="ACFOF2_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC7107914.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4026..4307)
                     /locus_tag="ACFOF2_001638"
     CDS             complement(4026..4307)
                     /locus_tag="ACFOF2_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107915.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4394..4717)
                     /locus_tag="ACFOF2_001639"
     CDS             complement(4394..4717)
                     /locus_tag="ACFOF2_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107916.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(4856..5035)
                     /locus_tag="ACFOF2_001640"
     CDS             complement(4856..5035)
                     /locus_tag="ACFOF2_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107917.1"
                     /translation="MPSERTFCRSDGIFISFSCLPPFENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(5051..6058)
                     /locus_tag="ACFOF2_001641"
     CDS             complement(5051..6058)
                     /locus_tag="ACFOF2_001641"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC7107918.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            6290..6526
                     /gene="acpP"
                     /locus_tag="ACFOF2_001642"
     CDS             6290..6526
                     /gene="acpP"
                     /locus_tag="ACFOF2_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC7107919.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6682..7929
                     /gene="fabF"
                     /locus_tag="ACFOF2_001643"
     CDS             6682..7929
                     /gene="fabF"
                     /locus_tag="ACFOF2_001643"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC7107920.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(8171..9124)
                     /locus_tag="ACFOF2_001644"
     CDS             complement(8171..9124)
                     /locus_tag="ACFOF2_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC7107921.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT         2275 a         2619 c         2402 g         2214 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatgtttaag agggcgatgt
      481 tgccgtagtt gtccaagagt tgcggatact ggcgggcatg ggcaagtatc agatattgga
      541 cgataaattc gacatcgacc acgccaccgc gcgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgataatttc gcctgctaag gcggtttggt
      661 cgcgttcggc agtcagcatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggtg
      721 tgccgcagat gaagcgggcg cgggtgaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 caacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggtg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gctctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttttcct
     1681 gccatgccca ttgccattcg ctggtgtcta gggtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttggtgcggt gggcattgag gtcgtctgaa aaagcggcgt
     1801 agctgtcgaa acccatactt tcggcaagca gttgctgctg ttcgggcgaa gccggcaggg
     1861 tttgggtttg ccggtcatcc cagtattgca ggcggtgttc gacatcgcgc aggaatcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagtttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcacgcat ttggccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagc ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc tgatttggcg gtgcagctta cgcatcgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacgaaggg gcgcaccagc gatttgattc
     2221 cgttcggata cggcgtaacc acgcggcctt tgcaccacgc gtagcgttcc cattcgcgcc
     2281 cctgcgtaat caggtattgc tccagcgcgg tttcgctcaa caccaacgcg cccgaatcac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgccc gtcggcggtg atgtcgttca
     2401 acagcgcaat cagtttctgc ccgactttgg tgaaaaactc ctgattgccg cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggataga cgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tataacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt tatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccagcatagg caggaaaatt tcgggcttga
     2881 gtttgccgtt gtcgagctgg cgggcgagga agagggaatg gcggcgggcg gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttgc cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaatagc
     3181 ataagccgca cttttctccc gtgtggaatt tttaaaaaaa ccatcactct tgacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattgcc
     3301 ggcttcaatc attccctctc ccactgcttt tgctgctttt gccacaccat tccaaaaacc
     3361 cataatcatg tcctccaatc gtttcaaata aataaggtcg ttgtttctgt caagaaacaa
     3421 cgacagttta taaactgtcg gaaaacaaaa tgccgtctga aggccggata atgcttcaga
     3481 cggcatcgga ttgccggttt taaccctgcc ctccgccgtt gttcaaatag tgttcccagt
     3541 gagaatataa ccgggcaata ttttcttcat aggtgtgggc agaaggaata taaccgtctt
     3601 tgggcggcat atacctgaca aatgattcta tttcttccgg ataatcaaaa tctgcataaa
     3661 ttttttcaac ttcccccaaa ggatcctcaa attcttcttt tctatggaat acatcgctta
     3721 agaggacgta cagccaattt ttaggggaat aatccctcat ttccgaagcc aaatcttcca
     3781 gaacaggctt taattcaaga atattggatt tgttgattaa agatagtttg aacacacgtt
     3841 catcatgatc tgaaagcgtc atttttcggg cataagctgc cacatcagcc caacccaagt
     3901 atttattccc atacccccac agaatatcct tccaagaaag acggactttt tgcgcggtta
     3961 aatcaaggtt cattttttac tccgattgcc tgccccgtca ttccgcccct tccgattgac
     4021 gcctgttacg aaaagtcttc caaatgacgg ctgattgcct cttcaacagt cggatgccaa
     4081 tccataaatg aagcctgcaa ttcctctaaa accaactcat tgaataaatc atccaaattc
     4141 cgccaatccg ttttttcatc cggatagatg aaatttgcca taacgacagt atgtttgttt
     4201 tgggaataat ccgataaggc gcaaatcatc agataccgcc ccgaatgctc cagataaaaa
     4261 attcgttttt ccaaaggaaa ccggttattc cgttgcccgt tttccatttt tcatcctttt
     4321 taacgtatcg gcttccgcgt ccttcaaatt gagttacctg ccttccgcca agcaaacgcc
     4381 cgcgcccctt tcatcatttg aatatccgtt tgaccatatt taccagataa ctatcttctt
     4441 tatataaatc aatcagcaaa ggcacgatac ccggctcata aaataattct gtcgtatcag
     4501 atgtttcttt taacgcggaa attatctcca acagttcata cccgtatcgg ggataagcaa
     4561 acaaataggg gactgcttcc cgcaaacatg aaaatgcttc gtaactttgc atttcccata
     4621 tatgttcatc tttaaaatca ctcaaatacc attcatccaa ttcactttcc ggatcggata
     4681 tcaatcggat aaattcattt agttcgtcct taaacatatc aaatccttaa atataaaatc
     4741 ttttgattat ccgccgtatc cttattttcc acatcatgaa tttcaacgcc gttcatattt
     4801 tgcgttttct caaaccgtcc accacggcat aaaattattt tttcatatca attatttata
     4861 tgtttaacaa cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg
     4921 cgggcgtttg ctttgtttca aacagggcgg cgcgtaaggg attttcaaag ggcggcaggc
     4981 aggaaaagga aataaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgt
     5041 gcgggacgcg tcattgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt
     5101 atatcaatcc gctgtacacc tggacggcag ttgcgcccaa gcggattttc tctgccgcgt
     5161 ccctgccttc cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg
     5221 ccaacagctt caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct
     5281 gctcgcctgc gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga
     5341 tgatgccgtc catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat
     5401 ccaaatcggg ggcgattttg acggcgagcg ggacgtattt cccgtgtaca gaggcaagct
     5461 gtgcctgttt gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg
     5521 cgcggaggtt tttagtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt
     5581 aggctttttc gaggcagatt aaataatcat cggcagcgtt ttcaatcggt gttacagcgt
     5641 ttttgccgat gttgatgccc aatacgccgc tgaatttact tttttcgatg ttttgaatca
     5701 tggcatcgat gccgtggttg ttgaagccca tgcgattgat gatgccttga tattcgggaa
     5761 cgcggaagag gcgcggctgc gggttgccgg gctgcgggtt gggcgttacc gtgccgattt
     5821 cgatgaaacc gaagccgagc gcgcccaatg cgtctatata ttcgccgttt ttatcgagtc
     5881 cggcggcaag cccgacaggg ttgggcaaat ccaaacccat cagttttaca ggtttggtac
     5941 ggttgccggt tacgggaatc agacccaatt tataaaccat gttgagcgcg tcgagcgtga
     6001 agtggtgggc tttctcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     6061 cagggctttc tgcttcagat ggaaatgccg tcattttaac cgaaatcccg tcaaacatac
     6121 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca
     6181 aatattgata taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg
     6241 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     6301 cgaacaacaa gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa
     6361 aaacgaatct tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt
     6421 tatggctttg gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     6481 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     6541 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     6601 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     6661 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     6721 gtttcccctg tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc cggcaaaagc
     6781 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     6841 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta
     6901 ttcatccact acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat
     6961 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     7021 cccagcatcg aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     7081 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     7141 taccgcggcc cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc
     7201 aattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     7261 ggcgcaatca gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc
     7321 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc
     7381 ggcgaaggcg cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg
     7441 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     7501 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     7561 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     7621 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg
     7681 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     7741 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     7801 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     7861 gccatttcca actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc
     7921 aaaggctgat tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     7981 ggcattttta tagtgattaa caaaaatcag gacaaggcgg cgagccgcag acagtacaaa
     8041 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta
     8101 aggcggggca acgccgtacc ggtttaaagt taatccacta tatatcgtgg gacggttcgg
     8161 acacgcccga ctaagcgtcc cgacgcttgt cccacagagc gaacaagttg cccaataccg
     8221 tccccgaaac cgaatgccac acgcaggcga ccgccgccgc aaccgccgat tcggggttgc
     8281 ctgggaaaaa tttcgcgctc aatcctgttg ccaaaccggc attctgcacg ccgacttcga
     8341 ttgccaaggt gcgttttttt gccgtatcca taccaaacag cgcggcggcg taatagcctg
     8401 acacatagcc ggcgatattg tgtaccgcaa tcgccaacac cattaccaac gccgattcgg
     8461 caaaacggtg tccgtgaacc gaagccacgc cgccgacaat gcaggcaaat gccaataccg
     8521 ccacgcccgg cataacggca cggacatttt caaaccaatg ttttccgtgc agcaggatat
     8581 ttgccgccga acccagcacc accggcaaaa gcgtgaccag cagcataaac cggaacatcc
     8641 cccaaccgtc catatccacc gtctgaccga ccaggcaggt catccacaaa ggcgtaacgg
     8701 ccggagccag caccgtagac accgtcgtca taccgactga aaacgccaca tcgcctttgg
     8761 caaggaaact cataatgttg gacgacacgc cgcccggaca cgtccccacc aacaccagcc
     8821 ccaaagtcag ccccggcgaa aggtcgaaca gcttggcaat accgatggcg agcaacggca
     8881 tcacggtata ttgcgccgcc gcaccgataa acacatccaa aggacgctgc gccaaaatcc
     8941 ggtagtcctc cctgcccaaa gtcatcccca tacccagcat aatgatgccc agcaccaaga
     9001 cctgaacatc gcccttaacc cacgcaaacg aagccggtcc gataaacgca accgccgacg
     9061 caagcaaaat gaccaaagcc gtaaaccgcg tcaggttgcg gctcaaacca atcagaaaac
     9121 ccatcacaca cccctcccga aacggcgcac cttgcgccgg acattattac cgcccgtgca
     9181 aaaccgaccg acggccactc atcgtccccg ccccatttta ctaaccggaa ttaacagata
     9241 cagcacaaat attaacccga accgcaaacg cccatcaagc cctttctgac ggcatcggca
     9301 ggcataccgt tggctatatc caaagaatca ggaattattt agcccgaatc atccggtttg
     9361 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
     9421 cggaaccgat tcactcggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
     9481 aggcaacgcc gtactggttt ttgttaatcc
//
LOCUS       DAXIHS010000076         9156 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXIHS010000076 DAXIHS010000000
VERSION     DAXIHS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.076"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..309)
                     /locus_tag="ACFOF2_001645"
     CDS             complement(<1..309)
                     /locus_tag="ACFOF2_001645"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC7107922.1"
                     /translation="MTIKSGGIADGYKGRLKDVVWKTLGEVFDLKNGYTPSKSNKEYW
                     ENGSIPWFRMEDIRENGRILDNSLKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIK
                     V"
     gene            complement(281..877)
                     /locus_tag="ACFOF2_001646"
     CDS             complement(281..877)
                     /locus_tag="ACFOF2_001646"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC7107923.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(1043..2053)
                     /locus_tag="ACFOF2_001647"
     CDS             complement(1043..2053)
                     /locus_tag="ACFOF2_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HFC7107924.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(2050..3594)
                     /locus_tag="ACFOF2_001648"
     CDS             complement(2050..3594)
                     /locus_tag="ACFOF2_001648"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC7107925.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3667..4671)
                     /gene="rfaD"
                     /locus_tag="ACFOF2_001649"
     CDS             complement(3667..4671)
                     /gene="rfaD"
                     /locus_tag="ACFOF2_001649"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC7107926.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(4712..5831)
                     /locus_tag="ACFOF2_001650"
                     /pseudo
     CDS             complement(4712..5831)
                     /locus_tag="ACFOF2_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(5833..7068)
                     /locus_tag="ACFOF2_001651"
     CDS             complement(5833..7068)
                     /locus_tag="ACFOF2_001651"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC7107927.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(7105..8076)
                     /gene="hldA"
                     /locus_tag="ACFOF2_001652"
     CDS             complement(7105..8076)
                     /gene="hldA"
                     /locus_tag="ACFOF2_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC7107928.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(8131..8871)
                     /gene="pyrF"
                     /locus_tag="ACFOF2_001653"
     CDS             complement(8131..8871)
                     /gene="pyrF"
                     /locus_tag="ACFOF2_001653"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC7107929.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
BASE COUNT         2160 a         2042 c         2166 g         2788 t
ORIGIN      
        1 aacttttatt aacgcatgct ctcctatggt tgccgttgtc gacatcatta ttgatttagc
       61 aggaaacagc ttgccgccct taactgcgct ttttgatata tgcttcaatg aattatctaa
      121 aatacggcca ttttccctaa tatcttccat tctaaaccaa gggattgagc cattttccca
      181 atattcttta tttgatttgg aaggggtata gccattcttc aagtcaaaca cctcccccaa
      241 cgtcttccaa accacatctt tcagacggcc tttatagcca tcagctatcc cccccgattt
      301 gattgtcaaa atctaaaaga aagtcgcggt aataccggta ttggcgtttg cgcagggcta
      361 attcagcttc cagctcggtg aatttgtcaa gtatttttac aattttttgt tgggtttcca
      421 ggggtgggat agggattaat aattttttca agttactggc atttagagca ggaatacctg
      481 taaatgtaca taaggcatga atctcttgtt caaattcaag taatatatga tagagaaaac
      541 gaacatccaa ttctgttcta ttcttaatag taactgcata atttaagttg ccacgaaaat
      601 atcttccttc ttgccacgtt attgagccga ctcccgctcc tcttgttgtg atgccgatag
      661 ggtcattttc agtgttccat tcatcaatat agccaagcgg ctctttgcca ctgttaataa
      721 ctggataact accaatatta tttgagattt tctgcttact tacagcttgt cctgttttta
      781 tgatagctat cccattttct ccacccaacg gcttccactc caccggtgcc gtctgaatca
      841 tctcaatcaa ttttttcgct ttgctttgca tatccatggt tgttttccgt ttctttcccg
      901 ctgtggcggg atgtggggcg gttgtggggt gcttcggctc actctgtccg tttgccgtta
      961 tttctgcggg ctgaagtccg tcctgctttc aggctgcctg caatggtgtg caggctgctt
     1021 tttcaggtag cctgaatggt agttattttg tgcggtcttt tttctgtttg atctgttgca
     1081 cggctgtttt ttccaacgct ttaacgcttt ccagataggc ttgctccacc ggcgacagga
     1141 tgcgcgcttc ataggcgcgg tattcgcgtt cggctttctg ctctgcctgt ttgcggctga
     1201 tgctgcctgc accctgtaat gtgccttgtc cgtacagccc ggaaaatttg tccaattcgg
     1261 ctatccagtc gcgcatatac atggggcttt gctcctgcgc tttgatttcc gctaggtcga
     1321 agaaggcgga aaccagacgg ttcaggcgga acagttcgtc ttcggtcaga tagtttttgg
     1381 cgattttggc ttcattcagc gtggggattg cgccttgaaa ggtggtcagc cccataaagt
     1441 ctttgctgct gtcggcacgg ctgtatatca gctcagctgc ggtttgccgg ctggcggcat
     1501 agtgcagttt gttttgaacg gcggcaaaaa aggtttggct ttcgctgctt ttggggttgt
     1561 agtcttggct ggtggcatat aaatcaagca cttgccggta tagggctttt tcgctgctgc
     1621 ggatgtcgcg aatgcggttg agcagttctt tccaatagtc gccgccgcct gtgcctttca
     1681 ggcgttcgtc gtctatggca aagcctttgg tcagatattc gtccagccgt tcggttgccc
     1741 attggcggaa ttggatgccc cgcgcggaac ggacgcggta gccgacggca ataatcatgg
     1801 gcagggaata atgggcgatt ttgcggttta ccttgcgccc gttttcattt tgaactgtca
     1861 actggaagtt gacagttgcc ttctcttcca attcttgctc tgcaagaatg gttttaatgt
     1921 gtttgctgat attttgtttg gtggtttggt acagttctgc catgtccgcc tgcgtcagcc
     1981 aaagctgtcc gccaaattcc tgcaaggcaa attgggcagt gccgtcgttg gcggtgtata
     2041 ggatgatgct catgcttcaa tctctgcaat cacttcgtca atttcacgcc gcagccgttc
     2101 gattttggca acggtttcgc tgatttcggc gttaagctgt ttgatgtcga taatttcgcg
     2161 ggtgtcttcg ggttcaacat agctgctgac ggcgaggttg tagccgttgt ctttgacggt
     2221 ttgctgggcg gcgttttggg cgatatgcgg cacatcggct ttatcggcga agagtttgac
     2281 gatttcggca atgtgttctt cggttaagac gttgttgttg gtttcttttt taaagaagcc
     2341 gcctgcgtcg atgaattgga tgtcggtatt gtctttgtgt ttggacaaaa ccagtatatt
     2401 gacggcgatg ccggtgccgt aaaagagatt gggcgcaagg gcgatgacgg tttccacgta
     2461 gttgccctcc accagatatt ggcggatttt ctgctctgcg ccgccgcgat agaaaatgcc
     2521 ggggaatgag acgatggcgg cgcggcctct gccggaaagg tagttcagtg cgtgcagaat
     2581 gaaggcaaaa tcggctttgg atttcggggc gagtacgcct gcaggggcaa agcggtcgtc
     2641 gttgattaag gtggggtcgc cgctgcctat ccagttgatg gaatagggcg gattggaaac
     2701 gacggcatca aagggtttgc tgtctttgag tttgggattg gtcagtgtgt cgcccaattc
     2761 gatgtggaat ttgttgtaat tgacgttgtg cagaaacata ttcatgcggg cgaggttgta
     2821 ggtggtgtgg ttgatttcct gcccgaagaa gccttcttcg atgatgtgct cgtcaaactg
     2881 ttttttcgcc tgcaagagca ggctgcccga gccgcaagct gggtcgtaga ttttgtttac
     2941 tttctcctgc ccgtgcaccg ccagccgcgc aatcagcttg gatacgcttt gcggggtgaa
     3001 aaattcgccg ccggatttgc ctgcgttggc agcgtagttg gaaatcaggt attcgtaggc
     3061 atcgccgaaa aggtcgatgt ggtggtcttc aaaactgccg aaatcgagtt ccgccacgcc
     3121 ttttaggacg gcggcaaggc gtttgttctt gtcggcaacg gtgctgccga gccggctgct
     3181 ggtggtgtcg aagtcgtcaa acaggccttt aatgtcttgt tcggacggat agccggaggc
     3241 ggagctttca atcgcggtaa aaatttcttt cagcttggtg ttgagctctt cgttttgatg
     3301 ggcttcggcg gcaatattgc aaaaaagctg gccggggtag atgaaatagc ctttgacttt
     3361 gacggcatcg tctttgattt cgggcgtgat gatgctgtcc ggcatagcgg cgtaatcaat
     3421 actgctgtcg cctgcctgca tatagtcggt aaagttttcg ctgataaagc ggtagaaaag
     3481 tgtgccgaga acgtattgtt taaagtccca gccgtccacc gcgccgcgta cttcgtcggc
     3541 aattttccaa atttggcggt gcagttgggc gcgttgttgc atttcggtca tcatcgaaat
     3601 ccatataaaa gttaaacaaa tcgaaatcgc ctgatatttt cagacgattt tttacgggca
     3661 ttcaaattaa gccaaatttt ccagcatcca tttgacgtag cgctcgacgc ctgatttgac
     3721 atcgaaaaat tcttccgtat aacctgcctc acgcaatttg gtgatgtcgg cttgagtgaa
     3781 gctttggtat ttgcctttga gcgcatcggg aaatgggatg tagcggataa gttcttcttc
     3841 taccagctct ttcaagctca tttcgggttt gccttcggcg gcgcggcagg cgttgacggt
     3901 ggcggcggcg agttcgttga actgttggct gcggccggtg ccgaggttgt agatgccgga
     3961 aagttcggga tggtcgaaga agtagaggtt gactttggca acgtcttcga cgctgacaaa
     4021 gtcgcgggtt tgttcgccgt tgccgtagcc gtcgttgctg ccgaacaggt tgacgtaacc
     4081 gtgttcgcgg tattggtgga aatggtggaa ggcgacggat gccatacggc ctttgtgttg
     4141 ttcgtgttgt ccgtacacgt tgaagtagcg gaagccgacg acttgggcgg tcaagccttc
     4201 tttcatgcgg cggcgcaata cttggtcaaa caggaatttg gaatagccgt acacattgag
     4261 cggtttttcg agttcgcgct cttcacggaa gatttcgcct ttaccataca cggcggcact
     4321 ggaggcataa aggaagggaa tgcgttcgtc ctgacaccag tccaacaaat ccagcgtgta
     4381 ctggtagttg ttttccatca tatacaaacc gtcgtgattc atggtgtcgg aacacgcgcc
     4441 ttgatggaaa acggcttcga tgttttgata aggtaaaatg tgttccctca cttggcggat
     4501 gaattcatgt ttgtcgagat aatgggcgat ttcgcactcg gcaaggtttt tgaatttttc
     4561 gcctttgctc aaattatcga cggcaacaat gtcagtaata ccgcgttgat taagtgcttt
     4621 gacgatgttg ctgccgataa agccggccgc gcctgttacg atgatggtca tattcggttt
     4681 cctttgtttt gagtaaaatt aataaactta gttacaagaa atcatcgata ttgtcctgaa
     4741 acacaaattt gcgatgatat tccaaatatt tttctcttcc ttcgggatat attctcatct
     4801 taattttttg catttggatt gatattaagt ttttcccatg tgtaacggct tagtcgagta
     4861 ccgcaaatca aacgaccatc atccaagaaa gtaatataac cttgatcgaa caaccaatca
     4921 taagtcggcg ttaaagctaa cccatttaaa taatctaatg cctctttctc atttttctca
     4981 gtaatacaaa ccatataagg tttaatatga ctagccctta acaaaatctc atctgtaatt
     5041 aatgtaaatg gacattgcgg catatgattt ataacatcct tgcgatattt ctgctgccct
     5101 tttctacttt tctgtttaat gatttcttgc tctacgaatt gattaaattc tgaagccggt
     5161 atttctaatt tttctcttga taagagttgc gggtgcacga tagagcaaaa ttgataatcc
     5221 aaaaatattc ggaaataaaa taatggttct gaatcttcta tatctttgac aggcaatagt
     5281 ttaagaattg ataaataact aattttgggc aatacaatct tacgccaaat tgaccatata
     5341 tcatcctccg aacggatata acccctacgg taacctttag aattttccaa atggtctgat
     5401 acatcatgga ttgaaaagaa aatattttct gtaggcagat tattaatact taaaacgtaa
     5461 tcatcataaa accttgaaat atcatgatga tatttctgtt cttgtttgtg atattcaact
     5521 tttgcatcaa ccaaataacg ctgtaaattt ttcttattaa aaaaacaatt tttctgaatg
     5581 aaaggatatt ctaccctcct tccatttttt gtctcagtaa tcccccaatt ttcatactca
     5641 aaaaatgtac ttagttttcg accactatca tcaatataac tacctacata tttgcgtgct
     5701 tccccattac caccaaaaac agataacttg ttattcctgt gaataaaact atcttccgca
     5761 cgtaaatttt gaatactatc gacaataaaa tactctatgt tttcaatagt aattctttga
     5821 aaatccggca ttttactctc cataaagcgt aatatccatt tgttttaata tagcgattaa
     5881 tacatcaaca acaatactgt ttcccgcttg gcgatacatt tgagtatcac taactaaaat
     5941 tttaaaatca tctctaaaac ccattagccg caaacattcc ctaggtgtta atttacgaat
     6001 acgtccacga ttatgagtaa cataattatc aaccccggca cgatgcattt tatgcatagt
     6061 ttgcaataat gggcgagcta caggcaaatc agtttccgta ctggttttaa aattttttgt
     6121 tcctcctgcc aaaacataat ttttgatttt ttcagaaaga taatattttt cctcaacgtt
     6181 atttacatca aaaataaagt catcaaattc agattcgcgg gctgcctgaa aaataaaatc
     6241 accatgccaa ttaaattgtt gatttgcttt ttggcataag gcaatttctc cattaatctg
     6301 tgtataacgt ttttgtctat ttttagaact ggtaacaaat ttcacaccct tttcacgtaa
     6361 aaaatatttg ctatcagtat agtcctccaa aaaatcttgc atagtatgtt ctaattcaat
     6421 cttttcagga aactgaaaac catttatagg aggggtatga aagccaacaa caaaaatacg
     6481 ctcacgatgt tgaggaatcc cataatcctt actattcatt atttggaaat ataagtcata
     6541 accaagtgaa taaaaaacac tttttacaac tttccaagtt tttccattat catgattgag
     6601 caagcccttt acattttcat aaataaatat ctttggttgg acttcatcca caacgcgagc
     6661 aaattcatag aataatgttc ctcgtgtatc ttctaatcct gcacgtttgc caaccatgga
     6721 aaatgcttgg caaggactgc ctccaactaa aatatcaact tgatttctaa actttctcgc
     6781 atcaaattga gtaatgtcgt tatgccaaaa atcttcattt aatttatagt ttccaagata
     6841 acttttttta acgtatggat caatatctcc tgcaaaaaca atggtatggt ttaaatttaa
     6901 tcggtggaat gcctgttcaa ccgcaccaat cccgctgaat acggttgcta atctaatatg
     6961 tgaatcaggt ttaagaaaag ttttagattt ccaaccttgt tgactgggaa agagcaaagt
     7021 tttttgtaat cgagtatcgt gtgtctgtgc cattgtcgaa atagtcatac ttatatcgtt
     7081 ctgtttatct tatcaatatg aaaactacat tgttgattgc cctgacaatg ccttgatcaa
     7141 ttcggcaaac gagcaaaccg ccgtaccgag tttcgccacg acaaccccgg ccgcagtatt
     7201 ggcaaggtac atggcttcgg gcatggtgca gcctgccgcc aaacccaagc ccattccggc
     7261 aatgacggtg tcgcccgcac cggatacgtc gtaaacttct tgggcgcggg tgggctggta
     7321 aatcggttcg ccttcgctga acaaggtcat gccttcttcg cttcgggtca gtaaaacggc
     7381 ggtcaggtcg aggtggcggc gcaggttttg cgctttttcg gtcagctcgc tttcgttttt
     7441 ccaactgccg accacttctt tcaattcggc gcggttaggc gtaatcagag ttgcaccgac
     7501 atatttttcg taatcgtcgc ctttggggtc gattaagacg gttttgccgg cgtgtttcgc
     7561 ccaatcgatc atatcggaga tatgcgacag gccgcctttg ccgtagtctg aaaaaatgat
     7621 tgcgtcgtat tcgggcaaga tttcgcggta tttctgcttg atttgttcca acacttcgca
     7681 gttgggatgt tcttcaaaat caagacggat aagctgctgg ttgcgggcga cgacgcgcag
     7741 tttgacggtg gtggcgattt gtttgtcgcg catcagatag gaggcgacgc cgtcctgcac
     7801 catcagcgca tcgagcgcgt cggcggcttc gtcgttgccg gttacggaca acagccctgc
     7861 cctgccgccc aacgaagcga tgttgcgcgc gacatttgcc gctccgcccg cgcgttggtc
     7921 gattcgtccg attttcgcca ccggcacggg ggcttcgggc gaaatacggg acacatcgcc
     7981 gaaccaatag cggtcgagca tcacgtcgcc gacaaccagg actttggctt gcgcgaaacg
     8041 ggatttgagg gtttcttgtt ggaacttggc ggacatattg cctccgttgt attttttcag
     8101 acggcctcag cctgtaaggc cgtctgaaaa tcagtttgct tcaaggtttg ccacgcggtt
     8161 cacttcgcgc aatacggcta ccggatcggc agcttgggtt acaggacgac ccattaccaa
     8221 ataagtcgaa gcggcagcca aggcttcggc cggtgtcatg atgcggcgct ggtcatcatt
     8281 attgccggca acgtccaagc ggatgccggg cgtgaccaag acaaaatctt gtcccaactc
     8341 gcggcgcagc ggtgcggctt cttgggcgga acagaccacg ccgtccaagc ccgaactttg
     8401 cgccagtttt gccaagcgga tgacttgttc ttcaggggcg gtgttcaaac cgatttccgc
     8461 caaatcgctt tgttccatgc tggtcaacac ggttacgccg attaagagcg gctttgtacc
     8521 gtatccggca acggcttctg ctgcggcttc catcatacgg cggccgcccg atgcgtgcat
     8581 atcgaccatc caaacgccca tatcggcagc gactttgcag gcttgcgcga cggtgtgggg
     8641 aatatcgtgg tatttcaaat cgaggaaaag tttgaaacct tgattgatta agctttctgc
     8701 caaattgcgt cccgtcgcgg taaacagctc tttgccgatt ttgatttgac acaatgtcgg
     8761 gtcaaggttg cggacgaatc cgagcgtgtc tttttcgttg gaaaaatcaa gggcgacgat
     8821 gacgggggtg cgttgttgcg gagtttggaa gtcggagatt aaaggattca tggtgttttc
     8881 gtttcgggtt ttcagacgac ctttatcgtc tttttcgcag tatagtggat taactttaaa
     8941 ccggtacggc gttgtctcgc cttagttcaa agagaacgct tctctaaggt gctgaagcac
     9001 aaagtgaatc ggttccgtac tgtttgtact gcctgcggct tcgtcgcctt gtcctgattt
     9061 ttgttaatcc actataacaa cgtaaattca aaccgtaggt cgggcattga tgtccgacct
     9121 acgcggactc aagcctccga caaactgttt cggata
//
LOCUS       DAXIHS010000077         8859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXIHS010000077 DAXIHS010000000
VERSION     DAXIHS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.077"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(203..1420)
                     /locus_tag="ACFOF2_001654"
     CDS             complement(203..1420)
                     /locus_tag="ACFOF2_001654"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC7107930.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAVS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1622..2350)
                     /gene="rph"
                     /locus_tag="ACFOF2_001655"
     CDS             complement(1622..2350)
                     /gene="rph"
                     /locus_tag="ACFOF2_001655"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC7107931.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2432..2896)
                     /locus_tag="ACFOF2_001656"
     CDS             complement(2432..2896)
                     /locus_tag="ACFOF2_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC7107932.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3113..3889
                     /locus_tag="ACFOF2_001657"
     CDS             3113..3889
                     /locus_tag="ACFOF2_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC7107933.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4027..4554
                     /locus_tag="ACFOF2_001658"
     CDS             4027..4554
                     /locus_tag="ACFOF2_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107934.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4725..5579
                     /locus_tag="ACFOF2_001659"
     CDS             4725..5579
                     /locus_tag="ACFOF2_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC7107935.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5620..6828
                     /gene="pncB"
                     /locus_tag="ACFOF2_001660"
     CDS             5620..6828
                     /gene="pncB"
                     /locus_tag="ACFOF2_001660"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC7107936.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTPY
                     PLANIREDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYHLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6920..8638
                     /gene="argS"
                     /locus_tag="ACFOF2_001661"
     CDS             6920..8638
                     /gene="argS"
                     /locus_tag="ACFOF2_001661"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC7107937.1"
                     /translation="MNLHQTVEREAAAAFAAAGISGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALAGNAVIESAEVAGPGFINLRLRPEFLARNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYNTLGLKLHPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLEEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPEDAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATVPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGATRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
BASE COUNT         2153 a         2439 c         2190 g         2077 t
ORIGIN      
        1 agcaccgagt gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttgtcct
       61 gatttttgtt aatccactat aaatttaatc cactatattt tttgttccaa agtcaaaata
      121 tgccgtccga acattcgggc ggcagacaaa acggcactgc ccgataaagg cagtgccgtt
      181 gtccgtttca aaccgtgaaa catcagccca aattaaaggc tttatgcaat accctggttg
      241 ccagttccat gtatttttca tcaatcaata cggaaacttt gatttcggag gtggaaatca
      301 tttggatgtt gataccctct tcggcgagcg tgcggaagat tttggcggct acaccgacgt
      361 gcgaacgcat acccaaaccg actgcagaga ctttgcatac ggtgtcgtcg ccatcaatag
      421 aaaccgcgcc gatactgtct tggcgttccg acaggatttc caaagtctgc ttgtaatcgc
      481 cgcgcggtac ggtaaaggaa aaatcggttg tgccttcgct gccgacattt tggataatca
      541 tatcgacttc gatgttggca tcggcaaccg cgcctaaaat ctgataggcg acaccgggtt
      601 tgtcgggtac gccgcgcacg ttgatgcggg cttggttttt atcgaatgcg ataccggtta
      661 cggcagctct ttccatgttg tcgtcctctt caaaggtaat taaggtgcca ttgccgccgt
      721 cttgcaggct gctcagtacg cgcaggcgca ctttgtattt tccggcgaat tctactgaac
      781 ggatttgcaa aactttcgaa ccgaggcttg ccagttcgat catttcttca aatgtaaccg
      841 tatccatgcg gcgcgcttcg ggtacgacgc gggggtcggt tgtgtaaacg ccgtctacgt
      901 cggtatagat ttggcactcg tcggctttga gggctgcggc aagcgcaacg gcggaagtgt
      961 cggaaccgcc gcgtccgagc gtggaaatat cgccttcact gctgatgcct tggaagccgg
     1021 caacgatgac gactttgcct gcatcgaggt cggcacgcat tttttcgtca tcaatgcttt
     1081 cgatgcgggc tttggtgtgg gcggtatcgg ttttgagggc gacctgccag cctgtgtagc
     1141 ttttggcatc cacgccgatg tctttcaatg ccatcgccaa aaggccgatg gttacttgtt
     1201 cgccggtgga caaaaccacg tccaattcgc gcggatcggg atgctcctgc atttcgtgcg
     1261 ccaacgcgac caggcggttg gtttcaccgc tcatggcgga tacgacgacg acgatgtcgt
     1321 gtccttcggc gcgggctttg gcgacacgtt tggccacgtt tttaatgcgt tcgggcgagc
     1381 ctacggatgt gccgccgtat ttgtgtacga ttaacgccat gtttcgtgct ttcttgtgtg
     1441 ggattggtcg ggcagcatgg tttgctggaa aagcggctat tattactatt ttttacatga
     1501 agttcaagaa cggactgcgc tttcccgcct gccgtttgac agcggtcagc gaaaaacctg
     1561 ttctttcaga ttgttgacaa aatgccgtct gaacggtttt cagacggcat ccggacgaca
     1621 atcaggcggc ggacaacgca ttttgctggt gttgcagcag ttcgcctatg cctttttgcg
     1681 ccagtgcaac cagtttgccc aattcgtcca aactgaacgg cgcgtcttcc gccgtcccct
     1741 gtatttcgat gatttttccc gatgcggtca tgacgatatt cacatcactg tcgcaaccgg
     1801 agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt cactacgcct actgacacag
     1861 cggcaacggc ttcgcggatg gggttttcac tcaaaatgcc gtctgaaacc agtttgccga
     1921 cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc cgtgcgcgta ccgccatctg
     1981 cctgaatcac atcgcagtca atcaagattt gtcgttcgcc gagtttttcc atatccacga
     2041 ccgcgcgcag cgaacgcccg ataaggcgtt ggatttcttg tgtgcgcccg gactgtttgc
     2101 ccgccgaagc ttcgcggcgc atccgggaag cagttgaggc aggcagcatc ccgtattccg
     2161 ccgttaccca gccttggttt ttaccgcgca gaaacggcgg gacgttttca tctatggaag
     2221 cggtacaaat cactttggta ttgccgcatt caataaggca cgaaccgtcc gtatgcggca
     2281 ggaaatgagg ggtgattttg atatcgcgca ggctgtccgc ggcgcgcgag atgcggatgt
     2341 aatcaggcat actgccctcc cgttaaaaac agataaatta aaaagcctta aatatgaaaa
     2401 atcacattta aggccttcaa actgaaaatt tctacgcctc ttcggctttg ctgcggataa
     2461 tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc aaaactgccc attaaaaggt
     2521 gcatcagtcc ggtacgtccg tgcgtgccca acaccagcag gtcggcaccg ttttcatcgg
     2581 cataatcaac caaatcctgc gccatttcac gcgcaccctt attggcaacc agcaggtgtt
     2641 tgacggtatt ttccacaccc agttcccggg cggtgcgctc ggcggcatcc aaaacttcgt
     2701 tgccttgcgc gacggcggcg gcttcgtagc tttcgtgttg caaaaattcg ggggcgagcg
     2761 ccatatattc ggcaggattg gcaacgtgta ccaaagtcag acgcgcaccg ttgaccccgg
     2821 caagctcggc ggcatgtttc agggcattga tggacgtttc actgccgtca acggcaacaa
     2881 ccaaatgttt gtacatatcg tattctcctt ttgcaccgcc tcgcggtgcc ctcttgtcgg
     2941 atgggcgcag ggacagtttg cgctgtttca ttatagaccc gccgtcgggc tttatacaac
     3001 agccgaacag cccgaccgct ttccagtata atatgccgct tccgtgcagt caggcatttt
     3061 ttgccggctt tcgttcactt tttgatttga cgcaatcttg caggattcga ccatgtccga
     3121 caacgctttg acctcttcgc gacgcttcgg cggcatcgcc agactctacg gagactctgc
     3181 cttggcgcac ttttcacagg cacacgtctg cgtagtcggc gtgggcggtg tcggctcgtg
     3241 ggcggtcgag gctttggcgc ggacgggcat cggacgtttg actttgattg atttggacaa
     3301 cgttgccgaa tcgaatgtca accgccagct gcacgccctg accggagact tcggcaaagc
     3361 aaaagttacc gccttgcgcg aacgcgtcgc ccaaatcaac ccagaatgcc aagtgtttga
     3421 aattgaagat ttcgttaccg aagacaattt gccggaatac ttcggaaaag gttttgattt
     3481 cgtcatcgac gcgatcgacc aagtgcgcgt caaagcagca atggcggctt attttgtgga
     3541 acgcaaacaa ccgtttgtcc tcagcggcgg cgcgggcgga caaaaaaatc cggcgttaat
     3601 ccaaaccgcc gatttgagcc gcgtaaccca cgacccgctg cttgccaacc tgcgctacac
     3661 cttgcggaaa cgctacggat tcagccgcga tacgaaagca aatatgcgcg tgccttgcgt
     3721 gtattcgacc gaaaatatcg tgccgccgca gtctagggag gcttgttcgg cagatgccgc
     3781 tccgcaaggc ttgtcgtgcg ccggctacgg tgcaagcatg ctcgttaccg cttcgttcgg
     3841 gctatattgc gcacaggcgg cggtggaaca catcgcagac aaaaaataag caatgccgtc
     3901 tgaaacagga ttcagacggc atttgaacaa actatggtta tgatttaaga caacaaagga
     3961 tacggataaa aaataacata aaatatatga ttcctaataa tataccaagt atcggagagc
     4021 tatttaatgg aattcgttaa taatttagtt attttttcat ttttattact aatgcttatt
     4081 ccgatatttt ttgtagtata tggtatatac cataagatac gttatcgcaa aatatatatc
     4141 ctaagaataa gttttacatt attagtggta atactttgca gtatgtatta catatattgc
     4201 cgttatcttg accaacaaaa agtagcttat tattgcatag atgaacaatg tatttctatt
     4261 gttcatctat acaaagatta tggtataaac tctcccacat atgcgagaat ttacgcagga
     4321 aaaatattgt ttagatttca agtaagagct aaaaattacg ctgaattact tatggaagat
     4381 gatatatcaa ttagtaaaaa aattttgggg aataaattta tcatttatgg gtcgctacct
     4441 gtaatatacg gtaatgtaga taatattgaa gtaaaagaag ctactggtta tatagataga
     4501 gccagtactg attatattgt ctcaagaaac ttaaaattca gacatttata ttaattaaga
     4561 ggttttagca agaccgtatt cacaacctgc tccttttcaa aacatttgca tttaaaagcc
     4621 gttataatgc cgtctgaaca tctgcccgac cacattatgc gtgaatgccg gcagattgtt
     4681 ttcttttgta aacttatatt aaaatccact taccgattca cgccatgccg cccatccctg
     4741 ccccatccgc accatccgag cacactgtcg catgggtatt cggccaaccc gttaccgatt
     4801 tgccccagga tttgtttatt ccgcccgatg cattgaaagt cgtattgggc agttttcaag
     4861 gccctttgga tctactgctg tatctgatcc gcaagcagaa catcgatgtt ctcgatattc
     4921 cgatggtgaa gattaccgag cagtatctgc actacatcgc ccaaatagaa acctatcagt
     4981 ttgatttggc ggcggaatat cttttgatgg cagcaatgct gattgaaatc aaatcgcgcc
     5041 tgctgctgcc gcgtaccgaa gccgtcgaag acgaagaagc cgacccgcgt gccgagttgg
     5101 tgcgccgcct gttggcttat gaacagatga agctggcggc acaagggttg gatgcgcttc
     5161 ctcgtgcggg ccgggatttc gcgtgggcgt acctgcctct ggaaattgcc gtcgaagcca
     5221 agctgcccga agtctatatt accgacttga cgcaagcgtg gctgggtatt ttgtctcggg
     5281 caaaacacac gcgcagccac gaagtaatca aagaaaccat ctccgtgcgc gcgcaaatga
     5341 cggcaatcct gcgccgtttg aacggacacg ggatatgcag gtttcacgac ctgttcaatc
     5401 ccgaacaggg cgcggcttac gtggtcgtca acttcatcgc actgttggag cttgccaaag
     5461 aaggtttggt cggaatcgta caggaagtcg gtttcggaga aatccgaatc agcctcaatc
     5521 atgagggggc acattcagac ggcatttccg gcacacgggg cgggcgcgat gtgttctaat
     5581 acgccccaag ccgccaccag aaactgggag aaccgccgca tgacaggcat catacattca
     5641 cttcttgaca ccgacctcta caaattcact atgctgcaag tggttctgca ccagtttccg
     5701 cagacacaca gcctttacga attccgatgc cgcaatgcct cgacccccta cccgcttgcc
     5761 aatatcaggg aagacttgga agtcgaactc gacgcgctct gccaactgcg ctttacccat
     5821 gacgaactcg gctacctccg cagcctgcgt ttcatcaaaa gcgactttgt cgattatctc
     5881 gaactcttcc agctccaacg ccgctttgtc gaagtcggca cagacgataa aggccgtctg
     5941 aacatccgta ttgaaggccc gatgatacag gcgatgtttt ttgaaatctt catcctcgcc
     6001 attgtcaacg aactttactt ccgccggctg gaaacccctg ccgttatcga agaaggcgaa
     6061 cgccggcttc aagccaaagc cgcgcgcctc aaagaaatcg ccgccgcaca aaaccccgac
     6121 gaaccgccct tcctgatttc cgacttcggc acgcgccgcc gttatcatct tgcctggcag
     6181 gaacacgtca tccgcaccct gctcgaagcc gccccaagca tcgtacgcgg aaccagcaac
     6241 gtttacctcg ccaaaaaact cggcatcacc cccatcggca caatggcgca cgaattcctc
     6301 caagccttcc aagccctcga cgttcgcctg cggaatttcc aaaaggccgc gctcgaaagc
     6361 tgggtacacg aataccgggg cgatttgggc gttgccctga ccgacgttgt cggtatggat
     6421 gccttcctgc gcgatttcga cctctatttc gccaaactct tcgacggttt gcgccacgac
     6481 agcggcgacc cttacgtttg gggcgacaaa gcctacgccc actatcaaaa gctcaaaatc
     6541 gacagccgca ccaaaatgct gaccttctcc gacgggctgg acatcgaacg ctcttgggca
     6601 ttgcaccaat atttcaaaga ccgcttcaaa accggcttcg gcatcggcac caacctgacc
     6661 aacgatatgg ggcatacgcc cttgaatatc gtgttaaaac tggtcgaatg caacgggcag
     6721 tccgtcgcca agctgtccga ctctccgggc aaaaccatga ccaacaacag caccttcctc
     6781 gcctacctgc gccaagtgtt cgacgtaccc gaacccgaaa cgccgtaaac cggcagaaaa
     6841 agcgcacaat tcctgtttct gccgcataaa atcttttaaa ataccgcctg atttgaattt
     6901 aaccgaaaga ccgaacttca tgaacctaca tcaaaccgtc gaacgcgaag ccgccgccgc
     6961 ctttgccgcc gcaggcatct ccggcagccc cgttgtcttg cagccgacca aaaacgccga
     7021 acacggcgat ttccaaatca acggcgtgat gggtgcggcg aaaaaagcca aacaaaatcc
     7081 gcgcgaattg gcgcaaaaag ttgccgaagc attggcgggc aacgccgtca tcgaaagcgc
     7141 ggaagtcgcc ggtccgggct tcatcaacct gcgcctgcgt cctgaattcc tcgcccgaaa
     7201 cattcagacg gccttaaacg acgcgcgttt cggcgtggca aaaaccgaca agccgcaaac
     7261 cgtcgttatc gactattctt cgcccaatct ggccaaagaa atgcacgtcg gacacctccg
     7321 ttccagcatc atcggcgaca gcatttcgcg cgtgttggca tttatgggca acaccgtcgt
     7381 ccgtcaaaac cacgtcggcg actggggtac gcagttcggt atgttggtcg cttatttggt
     7441 cgagcagcaa aaagacaatg ccgcattcga gctggcggat ttggagcagt tttaccgcgc
     7501 cgccaaagtg cgctttgacg aagatcctgc cttcgccgac accgcgcgcg aatacgttgt
     7561 gaagctgcaa ggcggcgatg aaaccgtatt ggcattgtgg aaacagtttg tcgatatttc
     7621 gctctcgcac gcccaagccg tttataacac gctgggcttg aagctgcacc ccgaagacgt
     7681 ggcgggcgaa tcgaagtaca acgacgattt gcaagccgtg gtcgatgatt tggttcaaaa
     7741 aggtctggcg gtcgaagacg acggcgcgaa agtcgtgttc ttggaagagt ttaaaaacaa
     7801 agagggcgaa cccgccgcat ttatcgtgca aaaacaaggc ggcggcttcc tttatgcttc
     7861 caccgacttg gcgtgtctgc gctaccgcat aggccgtctg aaagccgacc gcctgctcta
     7921 cgtcgtcgac catcgccaag ccctgcactt cgagcaactc tttaccactt cccgcaaagc
     7981 aggctatttg ccggaagatg caaaagccga gtttatcggc ttcggcacga tgatgggcaa
     8041 agacggcaaa ccgttcaaaa cacgcagcgg cgacactgta aaactggtcg atctgctgac
     8101 cgaagccgtc gagcgcgcca ccgctttggt gaaagaaaaa aatcccgaat tgggtgcgga
     8161 cgaagccgct aaaatcggaa aaaccgtcgg catcggcgca gtcaaatacg ccgacttgag
     8221 caaaaaccgc accagtgatt atgtgttcga ttgggacgct atgctctcgt ttgaaggcaa
     8281 caccgccccc tacctgcaat acgcctacac ccgcgtgcaa agcgtgttcc gcaaagccgg
     8341 tgaatgggac gcaaccgtgc caaccgtttt gaccgaacct ttggaaaaac aactagccgc
     8401 cgagctgctg aaattcgaag acgtgctgca aagcgtggcg gacacggcgt atccgcacta
     8461 cctcgccgcc tacctctacc aaattgccac cctgttcagc cgcttctacg aagcctgccc
     8521 gattttgaaa gccgaaggcg caacccgcaa cagccgcctg caactggcga agctgaccgg
     8581 cgacacgctg aaacaaggcc tggatttgct gggcattgat gtgttagacg taatgtaaaa
     8641 ccgcaccgcc cgattgcgga caacagcccc gcaatcaacc ggcggaccat cctcatccga
     8701 atctgaaaaa agcggcgcga tacaccgtat ccgccgcccc tcccaaaatg cgaaacagac
     8761 aaacgccaag caagcaagca agcaagcaag caagcaaaaa attataaccc ccttcctgcc
     8821 gacgcacgca ctttccgcgc ggcgcattcc ccttttccc
//
LOCUS       DAXIHS010000078         8809 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXIHS010000078 DAXIHS010000000
VERSION     DAXIHS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.078"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..109)
                     /locus_tag="ACFOF2_001662"
     CDS             complement(<1..109)
                     /locus_tag="ACFOF2_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC7107938.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGF"
     gene            283..486
                     /locus_tag="ACFOF2_001663"
     CDS             283..486
                     /locus_tag="ACFOF2_001663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107939.1"
                     /translation="MPDGFLQQRGRRFVLQESGRANRKCGRDFILMQPHSGGMETPKT
                     TKTGFRRHFWQSRLKTCRIWVTR"
     gene            complement(476..1567)
                     /gene="ychF"
                     /locus_tag="ACFOF2_001664"
     CDS             complement(476..1567)
                     /gene="ychF"
                     /locus_tag="ACFOF2_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC7107940.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1680..3356)
                     /locus_tag="ACFOF2_001665"
     CDS             complement(1680..3356)
                     /locus_tag="ACFOF2_001665"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC7107941.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            3531..3962
                     /locus_tag="ACFOF2_001666"
     CDS             3531..3962
                     /locus_tag="ACFOF2_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC7107942.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4193..4888)
                     /gene="murU"
                     /locus_tag="ACFOF2_001667"
     CDS             complement(4193..4888)
                     /gene="murU"
                     /locus_tag="ACFOF2_001667"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC7107943.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4906..5406)
                     /gene="hpaC"
                     /locus_tag="ACFOF2_001668"
     CDS             complement(4906..5406)
                     /gene="hpaC"
                     /locus_tag="ACFOF2_001668"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC7107944.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(5521..5961)
                     /gene="nadR"
                     /locus_tag="ACFOF2_001669"
     CDS             complement(5521..5961)
                     /gene="nadR"
                     /locus_tag="ACFOF2_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC7107945.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6304..6813)
                     /locus_tag="ACFOF2_001670"
     CDS             complement(6304..6813)
                     /locus_tag="ACFOF2_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC7107946.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAILVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(6892..6967)
                     /locus_tag="ACFOF2_001671"
     tRNA            complement(6892..6967)
                     /locus_tag="ACFOF2_001671"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6932..6934),aa:Lys,seq:ttt)
     gene            7178..8257
                     /gene="apbC"
                     /locus_tag="ACFOF2_001672"
     CDS             7178..8257
                     /gene="apbC"
                     /locus_tag="ACFOF2_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC7107947.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         2031 a         2523 c         2273 g         1982 t
ORIGIN      
        1 gaaacccgcc cggcagccaa tccttttcga tatggaacac gatgacggac aagacggcgg
       61 cggcgcgcaa tgtgtcgatg tccgggcggt agggtaaggc ttggctcata atgtttttat
      121 agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat ctgtactgtc
      181 tgcggtttcg tcgccttgtc ctgatttaaa gttaatccac tatactcgaa acgcggcggc
      241 gcaaatgccg tctgaaaggt cattttgtac gggatattcg gaatgccgga cggatttttg
      301 caacagcggg gcaggcggtt tgttttgcag gaatcgggga gggcaaatcg gaaatgcgga
      361 cgggatttta ttttgatgca gccgcattcc ggcggtatgg aaacgccgaa aaccaccaaa
      421 accggcttca gacggcattt ctggcaaagc cgtctgaaaa cctgccgcat ttgggttaca
      481 cgttaaacaa aaagtgcatc acatccccat cctgcacgac gtattcctta ccttccacac
      541 gcattttgcc ggcttctttg gctttggctt cgccgccgag cgagacaaag tcgtcgtaag
      601 aaatgacttg ggcgcggatg aagccgcgtt cgaagtcggt gtgaatcacg ccggcggctt
      661 gtggggctgt gtcgcctttg tgtatcgtcc acgcgcggac ttctttgacg ccggcagtga
      721 agtaggtttg cagccccaaa aggtcgtaac cggcgcggat caggcggttc aggcccggtt
      781 cttccaagcc catttcggcg aggaactcgg ctttttcacc gtcttccaac tcggcgattt
      841 cgctctccat cgcggcgcac acggcgacga cgggggcgtt ttcttttgcc gccaattctt
      901 tcaggcggtc gaggtgcggg ttgttttcaa aaccgtcttc ggaaacgttg ccgacgtaca
      961 tggcaggttt ggcggtcagc aggaacagcg gtttgagcat cgcgcgttct tccgcgtcca
     1021 aaccgaagga acgcacgggt ttgccttcgt ccagatgcgg cagcagtttt ttgcacaaat
     1081 cgaccagctt ttgcgcgtct ttgtcaccgg aacgggcgcg tttttcttcg cggacgatgg
     1141 ctttttccac acttgccaag tcggcaagcg ccaactctgt accgatggtt tcgatgtcgg
     1201 caatcggatc gactttgccg gaaacgtgga cgatgttgtc gtcgtcaaag cagcgcacga
     1261 cattcacaat cgcatcggtt tcgcggatgt tggcaaggaa ctggttgccc aagccctcgc
     1321 ctttgctcgc gcctgcaacc aaaccggcaa tatcgacaaa ttcgacgatg gcaggctgca
     1381 ttttttgcgg attgacgatt tttgccaatt cggccatacg cggatcgggg acttcgacga
     1441 tgccgacgtt gggttcgatg gtacagaaag gatagtttgc cgcttcgata cccgattggg
     1501 tcagcgcgtt aaaaagggtg gatttgccga cgttgggcaa accgacgatg ccgcatttca
     1561 aactcatgtt ttttcctgaa aatagagaaa tttaacggcg gattatagca taccgccgcc
     1621 cgcgttccga aaaatgccgt ctgaaacggc ttcagacagc atccggtttc aaaaaccgtt
     1681 cagaacaagc cgtgaatcac gccgtgttcg tccacatcga ttttctcggc ggccggaact
     1741 ttcggcagac ccggcatctt catcatattg ccgcacagcg cgacgatgaa gcccgcgccg
     1801 gcggaaacgg tgatgccgcg cacggcgatg cggaagcctt cggggcagcc caacagtttg
     1861 gcgttgtcac tcaaagagta ttgggttttc gccatgcaga tcggcatttt gtccaagccc
     1921 agtttttcca gtgaagcgat ttcggcagac gcttccgcgc tgaaatcaac atcttccgcg
     1981 ccgtacactt tttgggcaat cgcacggatt ttgtctttga tgcccaactc gacatcgtag
     2041 gcgaaaccga agttattggt ttgactttca atggcgttga cgactttgcg cgccaaatcc
     2101 gcgccgcccg caccaccttt gccccacact tcggtcaggg aaacttcaac gccgtgttcg
     2161 gcacaggctt tttcaatcat cgccaactcg gcatcggagt cggacacgaa gcggttgagc
     2221 gcaacgacga cgggcagtcc gaatacgttt ttcaggttgg aaatgtgttt cagcaggttg
     2281 ggcaaacctt tttccaaagc gtctaaattt tcttcgccga ggttggcgcg ttccacgccg
     2341 ccgttatatt tcaacgcgcg gacagtcgcc acgacaacag ccgcatcagg tttcaaaccg
     2401 gcaaggcggc atttgatgtc gcagaatttt tccgcgccca agtccgcgcc gaagcctgct
     2461 tcggttacgg cgtaatcggc aaggtgtttc gccagacggg ttgcggttac ggagttgcag
     2521 ccgtgggcga tgttggcgaa cgggccgccg tgtacgaagg cgggcgtgcc ttcgatggtt
     2581 tgcaccaagt tgggcttaat cgcatcttta agcaatgccg ccatcgcgcc attcgctttc
     2641 aaatctttgg cgtaaacggg gctgccgtct ttggcgtagg cgacaaggat gttgcccaaa
     2701 cgctctttca aatcgctgat gtctttggca agacagaata ccgccatcac ttcggaagca
     2761 acggtaatat cgaaaccgtc aggacgcatc acgccgtcaa caggcttgcc catgccgtcg
     2821 atgatgttgc gcaactggcg gtcgttcata tcgaccacgc gccgccacag cacgcgtttg
     2881 gggtcgatgt tcaactcgtt gccttggtag atatggttgt cgagcatcgc ggcaagcaga
     2941 ttatttgccg caccgatggc gtgaaaatct ccggtgaagt gcaggttgat gtcttccatc
     3001 ggcaaaactt gggcatagcc gccgcctgcc gcgccgcctt tcacgccgaa caccggccct
     3061 aaagaaggtt cgcgcagggc aatcacggca tctttgccga tgtggcgcaa cgcgtccgcc
     3121 aaaccgatgg ttacggtggt tttgccttcg ccggcaggag tcgggttgat ggcggttacc
     3181 aaaatcagcc tgccttgttt ttgcggcagt ttaaacgctt cggcaggatt gattttggct
     3241 ttgtaatgac cgtaaggctc aatgttgtcg gcgttcaggc ccagcttggc ggcaatttcg
     3301 ccaatcgggc gcatggtgga agactgggcg atttcggcat cggttttgaa gctcatgatt
     3361 ttcctttaga aatgaggagg gacataccgt ctgaaagcat caggcgacaa acaggtggat
     3421 tgaaaataat atcaggcata ttataacgtt atccgcacca aacccgcagt gaaatttttg
     3481 acgctgcaac aaaaataccg ttcatattgt tcacaatcca aggagaaaac atgggcagca
     3541 acgcatggct gttttgggca ttggcatcgg caggcttcgc ctcattgacc gccgttttcg
     3601 ccaaaatggg cttgcagggt atagattcag atttcgctac cttcatccgc accttggtca
     3661 tcctcgccgc tttggtactg tttttgacct acaccggcaa atggcagggc gtgaacggct
     3721 ttacggggca caactggaca ttccttatcc tatccggcct tgctaccggc gcatcttggc
     3781 ttgcctactt caaagccctg caactgggta acgcctcgca agtcgccccc gtcgataaat
     3841 tcagcctggt tctggtcgcg ctgatggcgg tggttttctt ggacgaacgc ccgaacacgc
     3901 aggaatggat aggcttgggg ctggtaacgg cgggcgtgtt ggtgctggcg ttgaaacgtt
     3961 aaaccgaatc cgccataccg cctgaaaccg ggttttgaca atctgacggg cggcggtctg
     4021 aacaaagtta cccaataaaa tccgacaccg cgccgacggc aaaccgccgc gcttcagatg
     4081 gcatttgttg gacggaaagc cccgaacgcc ctattttcac gcctccacga aaatatgccg
     4141 gacatccggt cggaaccgtt cggcagccgg ctcggcaagc caccgtttta tttcacttcc
     4201 aagcccctgc aagggcttga gcctctttca gacggcatac cgtgccgaca tccagccaca
     4261 agcccgtatg cttctgaccg ctcacgcggt tttgccgcat ttcgttccgc aatacgggcg
     4321 cgagtttcgc cacactgccc gcttcgattc cgtcaaacat ttcaggacgg taaataccca
     4381 cgccgctaaa tgtcaatccg ttgccgccat ttacttccgg ccgcacgctg ccgtcgggca
     4441 gcagggaaaa atcgccgtca ggatggtgcg ggggattttc caccagccac agatgggcgg
     4501 aaatgtgctc gggcagggac gatgccgcct gaaacgcggc ggtaaaatcg atgtcggtca
     4561 acacgtcgcc gttgaccacc aaaaacgggg catcgcccaa cagcggcaat gcccgtgcga
     4621 taccgcctgc ggtttccaag ccgccttcag gttcgggcga ataggcgatg ttcacgccat
     4681 aagccgagcc gtcgcccaaa gcctcttcta tctgccgacc cagccaagcg tggttgatga
     4741 cgatttcggt aaaccctgcc tgcttcagac ggcataggtg ccaaccgatt agaggcttac
     4801 ccgccacatc gagcagcggc ttcggagtgg tatcggtcaa agggcgcata cgctcgccgc
     4861 gtcctgccgc cagtatcatc gctttcatat atatctgtcc gaatatcagt ctaaagatct
     4921 aaactgccgt ctgaaataaa gcagcgcggg acgtttgccc acgcaatttt taatttcgtc
     4981 gagcctgacg taaaacacaa aatgtgtgcc gatttcatgt tggccgacaa tatgcccgtg
     5041 cagatgcgcc aacgcgcctt ctatttcagg ttgtccggtt ttaccgtaat gccaaatatg
     5101 ataggcaaaa cgttcttcgg gtgaaagatt ggtcaatccg gcaaaatgtt cggccacatc
     5161 ctgatgttcg tctgccaacg tattgatgca caggctgccg ttttccgaca ggatcggaat
     5221 gattcgcgca ctccggttga tgcacagcat cacggtcggc ggctcgtcgg taaccggcgc
     5281 gaccgccgtc attgtaatgc cgtaacgccc tgccgcaccg tctgtcgtga tgacatgaac
     5341 gcctgccgcg caagatgcca tcgcatcacg gaacgaagtt tgaaaagttt tctgcaaatc
     5401 cgccattttt cccctttaaa ctgtccccta tataagaatg ctgcacacaa ggcacccccc
     5461 atgtgcagca gttctgattc aaaaagccgt cggtcggaca tttccgcgcg ttacggcgta
     5521 ttacgagttc aacgcatcct cgattttggc aagttctgcc aacaggtctt taagcagcag
     5581 cattttctcg cggcccagca cttcctcgat agcgtcgtaa cgctcgtcca cttcttcgcc
     5641 gatttcctca tacagcttct cgccctcggc agtcagcttc agaaaaacac gtcgttggtc
     5701 gttggaaggt ttcaggcgga caaccaaacc cgctttttca aggcgggtca ggataccggt
     5761 caggctgggg cgcaaaatgc acgcctgatt cgccaaatct tgaaagtcca gcgtgccgtt
     5821 ttccgccaaa agacggataa tccgccattg ctgatcggta atattcgcct gattcagaat
     5881 aggcctgaat tgggtcatca gggcttccct tgcctgtatc agactgatat tgatagacgc
     5941 atgttttgat tgggtaggca ttgttcaagt ctccaagtta tcgaaaatca aactttcaaa
     6001 ccgtcgggaa agcctgtggg cgtaattttt gatgcaaccg ttatataaca aaacgaacat
     6061 atagcaacaa tacgctataa accgcatcgg acgactgggt ataaaagact ttaattccga
     6121 taatcctatc taaaaatatt ttaatagtta tatcttagtc tattttccct acaatctcaa
     6181 caaagtatta catttactgt cgaaagaact ttcttccaga aaaaacaaaa gccgccgcat
     6241 cagacgagca aggtatatgc cgtttgattc tctacatagc gtaaagttta acaaaaacaa
     6301 aagttaaccg aaaacatccg cctgaaaaat tcgtgcgcgc aagccccaat aactgctgat
     6361 tcccgtcgta tagtgaacca ttttcccatt tttgaccaaa acgacggcag gcgttgcgac
     6421 aatccgccaa gaccttgcca aacccccgtc ctcatcgttg accgtcggaa agcccaagcc
     6481 ccgctttgcc atatacgccg ccacttcgga cgcgctgccg gaacgtaccg ccacgccgac
     6541 gaccggaacg ccgtccgccg ccaaatcatc gattatcggc gactgataac ggcacacgcc
     6601 gcaccagctt ccccaaaaat acaccaaaat cgccttatcc cggctaaact gtcccaaagt
     6661 cagccgctgc cccgacagca gggtcaaagg ccgccctgcc gcaccggccg gctcttcggg
     6721 cttgcgtatc caatccaaaa acagcgacac caataaaaac accaatgccg tctgaacggc
     6781 aaatttgatg cccgaaagca gtttcttttt catacgctct ctcaaacggt acgcccgcgc
     6841 aaccggcagg caaacaaaaa gccaagtctc aaaacttggc ttccggttat ctggtgggtc
     6901 gtgagcgatt cgaacgctcg accaacggat taaaagtccg ctgctctacc ggctgagcta
     6961 acgacccgat aagccgtgca ttatacagca ccatcctacc tcgtcaagca aattttacag
     7021 gcttaattgc agaccactgt ttgcacggga tattttgaca acggattttc acaatccgcc
     7081 gcataccgtg taaaagttcg cacaaggaaa agcaaaccgc ccgaaatcaa tgtacacttt
     7141 ccgcccgttt cccaaactgc acacagaaac acacattatg aacatacaaa acatccgcac
     7201 cctcctcgac accgtcgccg ttccgaatac ggcacgcacg ctcggcggcg aaaaggccgt
     7261 ccgttcggtc gaacagcgtt cagacggcat ccatatcgcc ctgcatttcg gcttccccgt
     7321 cgcgcacatt gcctcagaaa cagccgaccg catacaggaa atcctgatgc cgctgacagg
     7381 cgacacacac atccatctgt ccatggacac tgaaatcggc acacacaaag tccagcccgg
     7441 cgttaccact attaaaggcg tgaaaaacat catcgccgtc gcctctggca aaggcggcgt
     7501 gggcaaatca accaccaccg ccaaccttgc cgccgcaatg gcgcgcatgg gcgcgcgcgt
     7561 cggcgtactc gatgccgacc tttacggccc gagccagccc acgatgctgg gtgtggacga
     7621 ccgcaaaccc gatcagaaaa accaaaaact cattcccgtc gaatcttcag acggcataca
     7681 ggtcatgtct atcggctttc tcgtcgatac cgaccaagcc gtcgtctggc gcgggccgat
     7741 ggtcagccaa gccttgcagc aactgatgtt tcaaagcgag tgggacgaag tggattatct
     7801 tttcatcgac ctgccccccg gtaccggcga catccagctc acgctgtccc aacgcatccc
     7861 cgttaccggg tccgtcatcg taaccacgcc gcaggacatc gcgctgatag acgcgcgcaa
     7921 ggctgtggat atgttccgca aggtcaacat tcccatttta ggcgtattgg aaaatatgtc
     7981 cgtccacatc tgcaccaact gcggacacag cgaagccctg ttcggcacgg acggcggcaa
     8041 agatttagcc gcacgcctca acgtccccct gctcggacag cttcccctaa gcctgcccgt
     8101 gcgcgaagca atggacggcg gcacaccggc gcaactgttc gacgaacacc ccgccatcgc
     8161 ccgaatctac accgatgccg cattccaaat cgccctgggc attgccgaca aaggcaaaga
     8221 cttcagcagc cggttcccta aaattgtcgt cgaataaagc cgcgtccgaa accgcaacag
     8281 caatgccgtc ccaagccccg cgcctgccgg cgggcttcat agtcctctct ccccgtggga
     8341 gagggctgga gagagggcaa caagccgcaa ggcttgtatt ggggcggtta agctgttggg
     8401 aaagattgcc gcaacttgga gaatgccctc tccccggccc tcccccacag gggagggagc
     8461 ggattgcggc aggttttgcg gttgcaggcg gtttgaaaaa cagattggat ttacaaccgt
     8521 tgatgcaggt cgtctgaaaa gcaaaaagca gcctgcacaa cctgattagt ttacaaaacc
     8581 cttaacccca acagcctccc cgtcctctct ccccgtggga gagggctgga gagcgggcaa
     8641 caagccgaaa ggcttgtatt ggggcggttg agttgttggg aaaggttgcc gcaacttgga
     8701 aaatgccgcc tccccggccc tcccccacgg gggagggagt gggtttcggc agatttttcg
     8761 gttgcaggcg atttgaaagg caacttagat ttgcagctgt tgtttcagg
//
LOCUS       DAXIHS010000079         8797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXIHS010000079 DAXIHS010000000
VERSION     DAXIHS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.079"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(94..774)
                     /gene="tsaA"
                     /locus_tag="ACFOF2_001673"
     CDS             complement(94..774)
                     /gene="tsaA"
                     /locus_tag="ACFOF2_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC7107948.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            888..1523
                     /locus_tag="ACFOF2_001674"
     CDS             888..1523
                     /locus_tag="ACFOF2_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC7107949.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSTKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            1560..2528
                     /gene="waaC"
                     /locus_tag="ACFOF2_001675"
     CDS             1560..2528
                     /gene="waaC"
                     /locus_tag="ACFOF2_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC7107950.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVETYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTAPEAGRLKNLEQPYYAALHATSRDSKLWPVENWRALLQKLNEEQQCNIYLPWGS
                     EVEKTRAEQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            2754..3503
                     /locus_tag="ACFOF2_001676"
     CDS             2754..3503
                     /locus_tag="ACFOF2_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC7107951.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKTLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3514..4449
                     /locus_tag="ACFOF2_001677"
     CDS             3514..4449
                     /locus_tag="ACFOF2_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC7107952.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4512..5123
                     /locus_tag="ACFOF2_001678"
     CDS             4512..5123
                     /locus_tag="ACFOF2_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC7107953.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(5175..5492)
                     /locus_tag="ACFOF2_001679"
     CDS             complement(5175..5492)
                     /locus_tag="ACFOF2_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC7107954.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5650..6921
                     /gene="purD"
                     /locus_tag="ACFOF2_001680"
     CDS             5650..6921
                     /gene="purD"
                     /locus_tag="ACFOF2_001680"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC7107955.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6955..7557
                     /locus_tag="ACFOF2_001681"
     CDS             6955..7557
                     /locus_tag="ACFOF2_001681"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC7107956.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(7628..8281)
                     /locus_tag="ACFOF2_001682"
     CDS             complement(7628..8281)
                     /locus_tag="ACFOF2_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC7107957.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGWRKGRCGRGAAGKVVVFDILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSRQLKILQDWCGI"
     gene            8296..8652
                     /locus_tag="ACFOF2_001683"
                     /pseudo
     CDS             8296..8652
                     /locus_tag="ACFOF2_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2325 a         1745 c         2308 g         2419 t
ORIGIN      
        1 ttaatccgct ataacttgat attgcggctt atggcgaaca aacaacagcc gtttgttcaa
       61 ctgctatgcg gcaaaaccgg atttttgccc gatttaaagc ggggttgggg aaagatcaat
      121 aacggttgca cggttttcct cgatttgaaa tctgacttcg taatctgcaa tattcatcac
      181 ataaatccgt tcgggaatat tctgataggc ggggcgcgga tcttgggcaa tgctttggct
      241 gataaggttt ttggtgtttg cagataaatt ttccgcgccg atgttttcct gccaaacgac
      301 ttccaactct acgggtttgc cgctgacgaa accggatgcg gcatcgggtt tggattcgac
      361 aaaggggata taaggtttga tgtccacaat cggtgtgccg tccagcaggt ctgcgccgct
      421 gcaatagagg cggacgggtt tgccggtttc gatgcgttcg agtttcagga gcgagagtcc
      481 gagatggttg gggcggtggg ggctgcgcgt ggcgaacacg cccatttttt gtttgccgcc
      541 gagccgtggc gggcgcacca tttgcgccca gccttcatcc aatacgccgt gaaaaataaa
      601 acttatccac acataatcga aatcttccag cccgcgcacg ctgtctgcgg tgaatttggg
      661 attcagctcg atgcatactt ctgcggcaga gaccaaaccg ggctggcggg cgatgccgaa
      721 tttctgtttg tagggcgagc gggcggtgcc gatgggggta atggtataag tcataaggtt
      781 gtggataagt tttttggaaa aaccttatct tatcacaatc ggttttaccg cttttgggca
      841 gtaatttgtt ataatacagg gcattttaat cgcgttgaaa taatgagatg attgtttcca
      901 ttgatgttga tgcacaaaaa acgtttacgc cactgtgtcc tgacgaactg cccgtgaacg
      961 aggggcattt gattgtcgag gagttgaatg cgcaagccgc tttggcggat ttgcgcgtga
     1021 tgacgaaaga tgcgcatcat atggtagcga aatggcttgt ggataaccct gttgatatgt
     1081 tgaagccgac aggtttgcct gatgcggatt tgacttgggt ggctcatgcg atggtcggta
     1141 cgcgcggcta tgaattattg gacggactgc cttctaccaa agaatacgat tattgcgttt
     1201 ggaaaggtgt tgatcctgaa ttgcatcctt acggcgcgtg ttttcacgat attgaggaaa
     1261 aactaagcac agggctgatt gaatggctgc gttgtcaaaa tacggatacg gttattatcg
     1321 gtgggttagc tacggattat tgtgttaaaa caacggtttt gcagttactc aaaggcggtc
     1381 gttggcaggt tgtcgtcaat gaagcggctt gtcggggtat tgcgccggaa accatcgaaa
     1441 cagcatggca ggaaatgcat tctgctgggg caatcatctt aaaaaacgct gatgaaataa
     1501 ataaatatat taataatcaa taagttaaaa ttgtttcacg tgaaaccccc ttcctaaata
     1561 tgaaaatttt gcttgtccgc ttgtctagta tgggcgattt aattcacact ttgcccgcaa
     1621 tcgaagattt ggcgcgacaa tgtcctgatg tagaactgca ttggctatgt gaggctggat
     1681 ttgcagatat tgcgcgcctg catccgtttg taaaaaaaat ccatgtgatg aaatggcggc
     1741 aatggcgcaa acatctcttt cgggctgaaa cttggcggga aatgggtcat ctgaaacaga
     1801 ctttgcggca ggaagtattt gatttcgtat tggacagtca aggtctgatt aaaagcgcgt
     1861 gtttcgccaa aatggcaaaa tccccgattt gtggtttgga taaaaacagt gcgcgcgagg
     1921 gatgggctgc tttggcgtat gtagaaacat acgctgtacc gaagggaaaa aatgccgttt
     1981 ggcgcaaccg tgaactgttt gcccaagtat ttgggtatgt aatgcctgaa acgcaggtat
     2041 ttggcttgac tgctcctgag gcaggtcgtc tgaaaaattt agagcagccg tattatgcgg
     2101 ctttgcacgc gactagccga gacagtaaat tatggcctgt ggaaaactgg cgggcgttac
     2161 tgcaaaagtt gaatgaagaa cagcaatgca atatttacct gccttgggga agtgaagttg
     2221 aaaaaacgcg tgccgaacag attgcagacg gactgccgtt tgctattgtg tgcgacaaaa
     2281 taaatttatt gcaggcagcg tatctgctga aatacgcggt cggaattgtc ggcgtggata
     2341 ccggtttgct gcatttggca aatgccttgg aaaaacctgt ggtcggcatt tataccgata
     2401 ccgatccgat taaaacaggc gttcaggttt cgccaattgc aaaaaatttg ggcaatatcg
     2461 ggcagattcc gaccgcagat ttggtttatc aaacgttgat ggattgtgtt gcagcagaca
     2521 aaggctaaag ggttgtctga aactgatata gcacctgata tttttcagtt tgttaagcat
     2581 aagaatgtat atctaccagc ctgagtaaat gctttaaaaa tggtataatc ggcaagcatt
     2641 atccgaaaga tttttgaatg aattcgtttt taaggaaaac ggatttggtt ttatagggaa
     2701 gaaggtttgg tttcgctgga tggtgaaagc agcgttcttt ttaggagggt tcaatgaaag
     2761 cactggtcgc agtaaagcgc gtagtggact acaacgtcaa agttcgtgta aaagccgatg
     2821 gttcggatgt ggatatcggc aatgtcaaaa tgtcgatgaa tccgtttgac gaaatcgctg
     2881 tggaagaagc tgtccgtttg aaagaagccg gaaaagtaag cgaaatcgta gcggtttctt
     2941 tgggtgagaa aaaatgcgaa gaaaccttgc gtacagcttt ggcgatgggt gccgaccgtg
     3001 ccattcatgt tgaaaccgat actaaactgg agtctctggc cgttgctaag ttgctgaaag
     3061 ccgttgcgga caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgatg
     3121 atgccaatca agtggcgcaa atgctggcag ctttgctgaa tgcggcgcaa ggtacgtttg
     3181 cttcaaaagt acacattgaa ggtggtgaag tacagattgt gcgcgaaatt gatggcggcg
     3241 aagaaaccat agcattaaaa ctgcctgctg ttatcagtgc tgatttgcgt ttgaacgagc
     3301 cgcgctttgt caaactcccc aatattatgg cggcaaagaa aaaaactttg gaaaaactga
     3361 ctcctgacga tttggttgcc gacatttcac ctcgtttgaa aacggtgaaa tttgccgaac
     3421 ctaaggcgcg tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga
     3481 aaaacgaagc caaagtgatt tgaggaggct gaaatgagcg tattgattat tgtcgaacac
     3541 gacaacaaac agttgaatcc taccactttg catgctgtta ccgctgccgc taagctgggt
     3601 aaaatcgatt tattggttgc cggaagcggt gcatctgctg tagtagagtt cgcaaaacaa
     3661 gtggcgggtg tggaaaaagt tttggttgca gatgctgccc attatgccga aggtttggct
     3721 gaagagttgg ctccgctggt tgtcaaattg gcagcggatt actgctatgt tgcagcaacg
     3781 gcaactacat tcggtaaaaa ccttttgcct cgcgtagcag ccttattaga cgtaccgcaa
     3841 atttctgatt tgaccgaaat tgtggataac acgacttttg tgcgccccat ttatgcgggt
     3901 aatgcatttg aaaccgtgca agctgattca gaaaaattgg tgctgacctt ccgtgcgacg
     3961 gtttttgacg cagtggcggc gcaaggtggt aatgctgagg taattaatgt tgaagcaacc
     4021 cctgcccaaa acctgagccg ttttgttaac cgtcagcttt cccattccga tcgtcctgaa
     4081 ctgactcagg caaaagtgat tgtttccggt ggtcgtgcgt tgggtagtgc ggaaaaattc
     4141 aatgaagtgc tgacaccgtt ggcggatgtt ttaggtgcgg caatcggtgc atcccgtgcg
     4201 gcggttgatg ccgaatatgc gccaaacgat gctcaagtcg gacaaaccgg taaagtggtt
     4261 gcgccgcaac tctattttgc aatcggtatt tcaggtgcga ttcaacacgt tgccggtatg
     4321 caagacagta aggtgattgt tgcaatcaat aaagatgctg atgcgccgat tttcaatgta
     4381 gccgattacg gattggttgg cgatttattt gaaatcgttc cacaattaac agaagcattg
     4441 aaaaattaat gtttcacgtg aaacgttcag acgaccttgt tattaatgag gtcgtctgaa
     4501 aacatttcag catgattacg acttataaaa ccattacttc ctcgacgcag gctgagttta
     4561 aagataaagg cagccgtttt attgcatttg cctatccgat tcggacattg gctgatgtga
     4621 aaaaatacct cgatccgtta aaggaagagc atcataaagc acgacactgg tgctatgcct
     4681 atcgtttggg tgtggatggc atgcaatttc gtgccaacga tgatggagag ccatcaggaa
     4741 gtgctggacg accaattttg ggacagattg attcggtggg tattaccgac gttttggtcg
     4801 tggtcgtccg ctatttcggt ggtactttgt taggtgttcc aggtctgata catgcgtaca
     4861 aagaggcgac ggctcaagcg ttggcagttg cagaagtagt tgaaaagaat attgaaaaaa
     4921 ctgtttggct gaaatgtgaa tatccgtttt tgaatgaagc gatacgcatt gctaaacaat
     4981 atcaggcgga tgtattggag cgggatttgc agttggattg ccgattgacg gtcagtttgt
     5041 cgttggtaaa ttatgaggcc tgtgttgcgg cttggaagag tacacggcag attgaagtaa
     5101 atacggaaaa gccttttgaa tgaaaggtcg tctgaaatgc tttgtgatgg tatttcagac
     5161 gacctcttcg ctgactaatc ttcttgtgta tatttgtcta aggcatcgac gctggagcgc
     5221 aaatataacc aagccagttg atggaattcg cccagcgcgt tccatagtgc atcttcactc
     5281 ataatgctgt attcgccttc tgtgcgcaaa tccacctccg ctccatcttc aatagcccga
     5341 tgacaagcct gatattcgtg ggtataaaaa ttgtccatat cgcgaatcaa agcgacggca
     5401 tgtttccaac ggcggatgtc ttcttccaat tgcggtagta aatgacacca ttcgcggtat
     5461 ttgctttgga tttcatcaat acgtttttgc atggttgctt gtcctttttt aatttatgaa
     5521 taagttgaat aggttgcaga gtggaatgaa gcggtctttt gtttgattct actaaaattt
     5581 cttcagatgg gataaaagtc cctttgtatc gagttttcgt taaaagtaca tatttgtcaa
     5641 ggagatggca tgaaactgct ggttatcggt aatggcggtc gcgaacacgc gctggcttgg
     5701 aaattggcgc agtcgcctaa agtggaaaca gtatttgttg cgtctggtaa tgccggtaca
     5761 gcgattgaac ccaagctgca aaacatcgac ttgactgcgc atcaggattt gattgaattc
     5821 tgtcgtaaag aaaatattgt ttttaccgtc gtcggccctg aagcgccttt ggcggcaggt
     5881 gttgtggatg atttccgtgc tgcaggacta aaaatattcg gtccgacaca atatgcggcg
     5941 cagttggaaa gttccaaaga tttcgccaaa gcatttatgg cgaaatacaa tattccgacc
     6001 gcgcaatatc aaacctttga aaacgccgat gccgcacatg attacgtcaa tcagaaaggt
     6061 gcgcctatcg ttatcaaagc cgatggtttg gcggcaggta aaggcgtgat tgtggcgatg
     6121 actttggatg aagcgcatgc tgcgattgat gatatgctgc tggacaacaa aatgggcaat
     6181 gccggcgcgc gtattgtgat tgaagatttc ctgcaaggcg aagaagcgag ttttatcgtc
     6241 atggttgatg gcaataatgt actgcctatg gcaaccagcc aagaccacaa gcgtctttta
     6301 gacggcgaca aaggtctgaa tacgggcggt atgggtgcgt acagccctgc gcctgtggta
     6361 acgcctgttg tgtacgaacg cgcgatgaat gaaattattt tgccgaccgt agcagggatg
     6421 aaagcggagg ggcatgagtt caccggtttc ctgtacgcag gtttgatgat tgatcaaagc
     6481 ggtgcacccc atacgattga gtttaactgc cgtttcggcg atcctgaaac ccagccgatt
     6541 atgagccgac tgaacagcga cttatcggat ttggttgaag cggcaataga cggcaaactc
     6601 gatagcgtaa ccgcggaatg gagcccgcaa actgccgtgg gcgtggtact ggcggcgcaa
     6661 aattaccccg aaacgcccaa aaaaggcgat gttatttccg gtctggatgc tgccaaccaa
     6721 gtaggcaaag ttttccatgc aggtacaacg gcaaacgaga aaggtgacgt attgaccaac
     6781 ggcggacgcg tattgtgtgt tgtgggattg ggcgataatg tggcgcaagc gaaagctaag
     6841 gcatatggcg cgttggaaaa aatcagtttc gacgggatgc aataccgtaa agacattgcc
     6901 gataaagcga ttaaccgtta attagaaaga ggatgatgcc gtctgaagcc aagtatgact
     6961 aatgtgcttc agacggtatt ttatattatg ttatttccga gaattatcgc agcatcagcg
     7021 caacgtaagt taagcgttta tttgaaaaaa ggtggtttgg tcgcttatcc gaccgaatct
     7081 tgttatggct tggggtgtct tccgacatta gccaaagcac tcggtaagtt ggcgcatttg
     7141 aaaaaaagac cgcaacacaa gggtatgatt gtcatcggga atcaatttga acaattgcag
     7201 cctttacttc aaatgccgtc tgaaaatctt caggatatgc ttagaaaaga atggcccgcc
     7261 cctaaaacgt tcctattatc cgccaaatcc tgtgtcttac ccgagttacg cgggaaacag
     7321 cgcagcaagt tggcggtcag ggttcccgcc catgtaggtg cgcgccgctt gtgtcaggct
     7381 ttgcaaacgc ctttggtctc gacttcgtgc aaccgcgcag gcaaacgcgc gtgtcggacg
     7441 gagagggaag tcagaaggca attcggacgc gatgtgtgga ttgttggtgg gagaatcggc
     7501 aggcagaaat cgccgagtca gattattgac ggggaaacag gtaaacgttt gcgttagata
     7561 tgggtacttg aatgaaaact tttttgcata cacaaagaaa aaattttggg gctgtactag
     7621 attagcccta aatcccacac caatcctgta ggatttttag ctgccgggac ggtgtgccga
     7681 agttaaatcg aaattcgcat tctttcaaga acagcgggaa agatttgcga tcgattccat
     7741 tgtattttcg caagacacgt tttgcctgat tccaaaaatt ctcaatgccg ttaatgtggt
     7801 tctgacggtc tgcaaattcc ttggaatggt tgatgcggta ataaatgaaa ccgctcacgt
     7861 ccaacttgtc gcagctgctc agactatcgg tataaacaat actgtccggc atgattttct
     7921 ttttgatgac agggagtaac gtttcagact tggcattgtt tacgacaacg gtatagaccc
     7981 gtccgttgcg tttcagaatg tcgaagacaa ccacttttcc tgccgcacca cgaccacatc
     8041 tgcctttacg ccacccgccg aaatcgcttt cgtccggctc gacaggatcc tcaaaaacct
     8101 catcggcagc cagggccaaa tgatggttga taaccgtgcg gattttacgg tagaacagtg
     8161 ctgccgaatt gggatggata cccaaaatat cggcggcaga acgggcggta acttccagca
     8221 caaaaaaacg gagcagttct ttctgtactt ttttctttaa tttgcagtgc gttatcttca
     8281 tatttcgagg gtaacatatc tgctaatcta gtacagcccc taaatttttc ggatggtatt
     8341 tctctgcatg attgttattg cacagagata aaggtagaag gacgcaacgt ctactttgat
     8401 tttgaggacg gttttgtcgt tccggacaat aatccgaaca atcaagcggg caagcatctg
     8461 aaaacagatt tttcccgtgt tgttttaacc cataaaaatg aaaacggcga agatgattat
     8521 ttggtcgata tttttgaaga catcgttttt ttagggaaac gtttgtttac ggtcagaaaa
     8581 tttcttgatt tttctgaatt gcttgagatg gttaatacac aaggttattt tcttgagttt
     8641 ctgtatttgt atagtggatt aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact
     8701 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatgagt
     8761 gtatcggtgt caaaacaagc gattattttt tagaggc
//
LOCUS       DAXIHS010000080         8139 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXIHS010000080 DAXIHS010000000
VERSION     DAXIHS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.080"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            445..1758
                     /locus_tag="ACFOF2_001684"
     CDS             445..1758
                     /locus_tag="ACFOF2_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC7107958.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILMPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1979..2136
                     /locus_tag="ACFOF2_001685"
                     /pseudo
     CDS             1979..2136
                     /locus_tag="ACFOF2_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2149..3399
                     /locus_tag="ACFOF2_001686"
     CDS             2149..3399
                     /locus_tag="ACFOF2_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC7107959.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3434..4927)
                     /gene="rng"
                     /locus_tag="ACFOF2_001687"
     CDS             complement(3434..4927)
                     /gene="rng"
                     /locus_tag="ACFOF2_001687"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC7107960.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEKTQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAASNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5140..5397
                     /gene="grxC"
                     /locus_tag="ACFOF2_001688"
     CDS             5140..5397
                     /gene="grxC"
                     /locus_tag="ACFOF2_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC7107961.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5418..5861
                     /gene="secB"
                     /locus_tag="ACFOF2_001689"
     CDS             5418..5861
                     /gene="secB"
                     /locus_tag="ACFOF2_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC7107962.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5947..7989
                     /gene="recG"
                     /locus_tag="ACFOF2_001690"
     CDS             5947..7989
                     /gene="recG"
                     /locus_tag="ACFOF2_001690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC7107963.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLQHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1724 a         2096 c         2396 g         1923 t
ORIGIN      
        1 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg
       61 tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctgcggc ttgccgcctt
      121 gtcctgattt ttgttaatcc actataaaat cccacgtgta cggcacgccc cgcgctccgc
      181 aatgataggg gcggtttcgt ttgaatcggc gtaccgggat gaatgccccc gcgccgtccc
      241 gataccggtt tgccggttgt tccgtatcgg ttgccgccgt gaaatgacgg acggatacag
      301 gaatggcggc aggcgtaaat gggaggaaat gaccgattga cttgtttcaa aataaaaaag
      361 tagaatgcaa ggcttatccc gcgtctgtgg gcggttttgc ctgcacggtc ggttttctgt
      421 ttaaaataaa ataaaggaga aatgatgctg acgtttatcg gactgctgat tatcggggtc
      481 atcgtatggt tgctgctgac ggaaaaagtg tcgcccatca tcgcattaat cttgatgccg
      541 ctgtttgggg cgttgctggc ggggtttgat gtatcccaat taaaagaatt ttattcgggc
      601 ggcaccaaat cggtgatgca gattgtgatt atgtttatgt tttccatttt gttttttgga
      661 atcatgaacg atgtggggct gttccgtccg atgataggcg gtttgattaa gctgactcgg
      721 ggtaatatcg tggcagtgag tgtggggacg gtcttggtgt cggtggtggc gcagttggac
      781 ggggcgggtg cgacgacgtt tttattggtc gtccccgccc ttttgccgct ttacaagcgt
      841 ctgcatatga atccttacct gctgtttttg ctgctgactt ccagtgcggg attgattaac
      901 cttctgccgt ggggcgggcc gactgggcgg gttgcaagcg tgttgggcgc agatgtgggc
      961 gaattgtata aacctttgtt gacggtgcaa attatcggtg tagtgtttat ccttgcgctg
     1021 tccctgcttt tgggtgtgcg tgaaaaaagg cggattgtcc gggagttggg cgcgttgccc
     1081 gccgtggcgg atttgataaa gccggtgcct ttgtcggaag aagaacaaaa attggcgcgt
     1141 ccgaaactgt tttggtggaa tgtcctgctg tttttggcgg cgatgagcct gcttttttcg
     1201 ggtatcttcc cgccgggtta tgtatttatg ctggctgcaa cggcggcgtt gcttttgaat
     1261 taccgcagcc cgcaggaaca gatggagcgg atttatgccc acgccggcgg cgcggtgatg
     1321 atggcgtcca ttattttggc ggcaggtacg tttttgggga ttttgaaggg tgcggggatg
     1381 ttggacgcga tttccaaaga cattgtgcat atcctgccgg acgcgctgct gccttatctg
     1441 catattgcca tcggtgtgtt gggcattccg cttgagttgg ttttgagtac ggacgcttat
     1501 tatttcggac tgtttccgat tgtggagcag attacctcgc aggcgggcgt ggcgcccgaa
     1561 gcagcaggtt atgcgatgtt gatcggcagt atcgtcggca cttttgttac gccgctttcg
     1621 ccggctttgt ggatgggctt gggtttggcg aaattgtcga tgggcaaaca catccgttat
     1681 tcgttttttt gggcgtgggg tttgtcgctg gcgatattgg ccagttcgat agcggcagga
     1741 atcgtgcctc tgccgtaaac ggcgaagccg tctgaagccc gaatcgttcc tgtggtctgt
     1801 cgcaggctgc ctgcctgccc gtgtgttgca atcgcgcccc aatgccgtct gaaggcagtt
     1861 gccggcgcgg tttttttgtg ctgcagtttt gaaaaatacc ggcaagcgta aaggccgcta
     1921 aattgcggct aattttgggg tgttagaata ggcacggttt gtctgaaagg gaaagttgat
     1981 gcgtgttttg ctggtggaag acgatgcgat gattgcggag gcggtgtcgg caagtttgaa
     2041 agacggcggc tatgcggtgg attgggtcaa aaacggcgcg cggctgccgc tgcccagcct
     2101 tatgacttga tgctgctgga tttgggtttg ccggggcgga cggtttggat gtttgttccg
     2161 ctggcaatgc ttgcaggtat gttttcctac tacgaaacct tccacgaaac ggaagcgttg
     2221 caggacgacc tgctccgtca ggcggcattg tacgtcgccc ccgattccaa acccgaaact
     2281 ttgcccgagg gcgacggcga tacgcgtatt ttggtacaga tgccgcagca ggaagaccct
     2341 gttgtcagcc tgcctgcgca tctggcggac ggtctgcaca cgcttcaggc ggacggggac
     2401 gacgattatt accgcgtcta tatccgcacg acggagcggg ggcggattgc cgtgatgcag
     2461 gaaagcgaat accgggaaga tttggcggcg gatgcggcac ggcaaagcgt gttgcccctg
     2521 ctggcggcac tgccgctgat gatactgctg accgtgtgga ttacgcacaa agccatgcgc
     2581 cccgtccgca aattgtcgca aagtctcgaa caacgccgaa tcaatgacct gtctgccttg
     2641 agtgtggaca atattcccag tgaaatcaga gggttcgtaa ccgccatcaa cttgcttttg
     2701 aaacgtgcgg atgaagacat ccgccaccgt cagcggtttg tcgccgacgc ggcacacgaa
     2761 ttgcgtacgc cgatgacagc cctttccctt caggcggaac ggctcaacaa tatgtctctg
     2821 ccacccgatg cggcgcggca gtctgccgtt ttgcagcaaa gcatacggcg caacaaacac
     2881 ctgctcgagc agcttttggc actggcgcgt tcgcagtcgg acgaaacccc tttggcgaaa
     2941 acgacattcg gtctgcaaag ccgtttccgc caagtgttgc aggaactgat gccgcttgcc
     3001 ttggaaaaac gtcaggacat tggtgtggcg gtcggaggtg atgtcgaaat atttgccgat
     3061 gaaactgaaa tctatacctt ggttaaaacg tttgttgata acgcaatacg ttataccccg
     3121 tccgaaggca ggatagattt gggtttcaca gacgaaggga aatatcttgc cgtgtgggtg
     3181 gaagataacg ggaacggcat tccggaatcc gaacgcgtcc gcgtcctcga tccgttttac
     3241 cgtattttgg gaacggagca gcaggggacg gggctggggc tgtcgattgc cgacacgcta
     3301 gccaaaaaat acggcggata tttggaactg accgacagcc gcaggttcgg acacggtctg
     3361 ctgatacgcg ccttgttgga caaggaaacc ctgaaataga cagatgccgt ctgaaggctt
     3421 cagacggcat tttttacatc agcacaatat cgtattgttc ctgcgtgtaa gcggtttcga
     3481 ccgccagaga aatcggtttg ccgatgaaat ctatcagcat tgccaaggat tgcgattctt
     3541 cgtccaaaaa caaatcgatg acgttggagg cggcgaggat gcggaaactt tcggcatcgt
     3601 aacggcgcgc ttcgcggacg atttcgcgct ggatttcgta gcacacggtt tgcggcgttt
     3661 tcagacggcc tctgccttgg caggaagggc agggttcgca gaggacttgg tttaagtttt
     3721 cgcgcgagcg tttgcgcgtc agctcgacca gccccaggct ggtaaaaccg tgcagggtaa
     3781 cgcgggtacg gtcgaaggcg agggctttgg caagctcctg caacacggct tcgcggtggc
     3841 tttcctgcgc catatcgatg aagtcgatga tgatgatgcc gccgaggttg cgtagcctca
     3901 attcgcgggc gatggtgtgg caggcttcga ggttggtgcg gaagatggtt tcgtcgaagt
     3961 tgcgtgcgcc gacgaagccg ccggtgttca cgtctatcgt ggtcatcgct tcggtggact
     4021 cgataatcag gtagctgccg aagttgaggt tgacgcgcgg ttgcagggcg cggctgattt
     4081 cctgctcgac gttgtgggtt tcaaacagcg ggcgttcgcc tttgaacaat tctatcctgc
     4141 ccaatgcgcc gtggacgtat tgttcggcaa aacgcgtcat ccgtccgtgg tttacggtgg
     4201 aatcgacgag gattttttgc gtgtcgcagc cgaccatatc gcgcaatacg cgcaaactca
     4261 agggcaaatc ctgataaagc agggtttccg gcggccggat tttcgcctgt tcttggatgt
     4321 gttcccacac tttggtcagg tagtcgatgt cggactggag ctgttcgtcg gtggcgtttt
     4381 cggcgttggt gcggatgatg tagcctcggc aggcattttc cggcaggagc ttgtcgaggc
     4441 gttcgcgcag gctgctgcgt tcggcatcgt cttcgatgcg ttgggacacg ccgatgtggt
     4501 cttcttgcgg aagatggacg aggaaacgcc ccgccagcga gatttgggtg gaaagccgcg
     4561 cgcctttggt gttgatcggg tctttgatga cctgcaccaa aacagactgc ccttcaaaca
     4621 gcatatgttc gatgcgctgg gttttttcgg ggttgcggcg ttgttcgagg acatcgacga
     4681 tgtgtaaaaa cgccgcgcgt tccaagccga tgtcgataaa cgcgctctgc atcccgggca
     4741 gcacgcggcg caccacgccc agatagatat tgccgaccag gctgtgttcg ctgttgcgct
     4801 cgatgtgcag ctcgcagata ttgttttcct ccaacaccgc tacgcgcgtt tcctgcggcg
     4861 tgatgttgac caatatcgtt tcgggcgggc gcgcgatgtc tttggggatg gggagtcctg
     4921 acaacatggt ttttcctgaa aatgtaataa aaatattttg catccgttcc gccgcgcagc
     4981 gcgggtgcaa aaatattttt cacaatgtct atcatacttt aaaaaagaaa ctttgacaca
     5041 ttccgcacat gccgtttgaa cgtgcatctg ttccgcactt gccaaacagg ggaaatgccc
     5101 ctatattgat tatcattgca aaactttcgg aaacccaata tgcagaccgt taccatgtac
     5161 acaggtccgt tttgtcccta ctgcgcgatg gcgaaaaggc tgctgcacgc ggcaggtgtc
     5221 ggacatatcg acgaaatccg tgtcgatgca agccccgaag cctttgccga aatgcagcag
     5281 ctttcgggac agcgcagcgt gccgcagatt ttcatcggcg aaacgcacgt cggcggattt
     5341 accgacctct accgcctcca gcaggaaggc gggctggacg gactgctgaa cccttaaccc
     5401 ccaactagga aaacaaaatg agcgaagaac tgcaacccgt attcagcatc gagcgactgt
     5461 atgtcaaaga cttgtctttg gaagtgccgc acgcgccgca aatctttttg gaacagggcg
     5521 agcccgaagt ggatatgcgc gtttccaccg gcagccaaaa gctggaagac ggctactaca
     5581 acgtggatgt aaccgttacc gtaaccgcca aattggataa cgagcgcacg atgtttttga
     5641 acgaagtaac ccaaagcggc atcttccgtt tggaaaacat ccccgaagaa gatgtgcagc
     5701 tgctgctggg cgtggcgtgt ccgaacatcc tcttccctta cgcgcgcgaa gccgtttcca
     5761 gtacggtaac gcgcgccggc ttcccgcccg tcctgctcgc gccgattaat tttgaggcga
     5821 tttaccaaca acagcaggaa gccgaagccg ccggggcttg attcctgcct gccgatgccg
     5881 tctgaatccg tttcagacgg tatttttgtt ttcggataaa ataggtgcgt tcaaaggaat
     5941 ctgcctatga tgtcgcccga aacccagaaa cagctcaaaa tcaccgatgt ttccgccaag
     6001 aagctcgaca aactcaacct ccataccgcg tgggatttgg tgttgcacct gccgctgcgt
     6061 tacgaggacg agacgcacat tatgccgatt aaggacgcgc cgattggcgt gccgtgccag
     6121 gtcgagggcg aggttatcca tcaggaagta acgttcaaac cgcgcaagca gttgattgtc
     6181 caaatcgccg acggttccgg cagcgtcctt tttctgcgct tcatccactt ttacgccagc
     6241 catcagaaac agacggcggt cggcaaacgc atccgcgccg tgggcgaaat caaacacggc
     6301 ttttacggcg acgagatgat tcatcccaaa atccgcgatg ccgagggcgg cggtttggcg
     6361 gaaagcctca cgccggttta cccgaccgta aacggtttga accagcccac tttgcgccgt
     6421 attattcaga cggcgttgga cgttacgccg ctgcacgaca cgctgcccga tgccctgctg
     6481 tgccgtctga agctgcaaca cctcgccgaa agcctgcgtc ttttgcattc gccgccgccg
     6541 agtttcacga ttcatcagct ttcagacggc acgctgcctg catggcaacg gctcaaattc
     6601 gatgaacttt tggcgcaaca gctttcgatg cgcttggcgc gacagaagcg tatcggcggc
     6661 acggcggcgg cattgggcgg cgacggcaca ttgacccaag ccctgcgcca agccctgccg
     6721 tttgccctga ccgatgcgca agaaaaagtt gtttccgaaa tctgccgcga tatggcgcaa
     6781 acctacccga tgcaccgcct gctgcagggc gatgtcggca gcggcaaaac cattgtggct
     6841 gctttgtctg ctttgacggc tatcgaatcc ggtgcgcagg tggctgtaat ggcgcccact
     6901 gaaatccttg ccgaacagca ttttattaaa tttaaacaat ggctcgaacc tttgggcatt
     6961 gaagttgtcc gcctttttgg cagtttgcgt aaaaaagcca aagacgaagc caaagccaaa
     7021 ctggctgacg gcagcgtcaa aatcgccgtc ggcacgcacg ccctgttttc agacggcgtg
     7081 gcgtttcaca atttgggctt gaccattgtg gacgaacagc accgtttcgg cgttgcccaa
     7141 cgcctcgccc tcaaaaacaa agggcgcgaa gtccatcagc tgatgatgtc cgccacgccc
     7201 atcccgcgca cgcttgcgat gagttttttc gccgatttgg acgtgtccgt catcgacgaa
     7261 ttgccgcccg ggcgcacacc gattaaaacg cgcctcgtca acaacgtccg ccgcgccgaa
     7321 gtcgaaggct tcgtcctcgg cacttgccga aaagggcggc aggcgtattg ggtctgccca
     7381 ttgattgaag aaagcgaaac cctgcaactg caaaccgccg ccgaaaccct cgcccggctt
     7441 cagacggcat tgcccgaact caatatcgga ctggtacacg ggcgcatgaa ggccgccgaa
     7501 aaggccgaag tgatggcgcg gttttcttcg ggggggctga acgtcttggt cgccaccacc
     7561 gtcatcgaag tcggcgtaga tgtgcccaat gccgccctga tggtcatcga acacgccgag
     7621 cgcatgggct tggcgcagct tcaccaatta cgcggacggg tagggcgcgg cgcggcggaa
     7681 agcgtgtgcg tcctcctgtt tgccgaaccc ttgggcgaac tcgccaaagc gcggctgaaa
     7741 gtcatctacg aacacaccga cggcttcgaa atcgcccgcc aagacctcaa tatccgcggc
     7801 ccgggcgagt ttctcggcgc gcgccaaagc ggcgtgccta tgctgcgttt cgccaaactc
     7861 gaagaagacc tgcacctttt ggaacaggcg cgcgaaaccg ccccgatgct gattgagcaa
     7921 aaccccgaaa tcgtcgaagc gcatttggca aggtggcttt ccggcaggga agggtatttg
     7981 ggcgtgtgag caaaatgccg tctgaaatat tagaaaaaaa agtgtaacgg aaatctgatt
     8041 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     8101 tacggaaccg attcacttgg tgcttcagca ccttagaga
//
LOCUS       DAXIHS010000081         8132 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXIHS010000081 DAXIHS010000000
VERSION     DAXIHS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.081"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..116
                     /locus_tag="ACFOF2_001691"
     CDS             <1..116
                     /locus_tag="ACFOF2_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC7107964.1"
                     /translation="NGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            329..1864
                     /locus_tag="ACFOF2_001692"
     CDS             329..1864
                     /locus_tag="ACFOF2_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC7107965.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLSG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1861..1953
                     /locus_tag="ACFOF2_001693"
     CDS             1861..1953
                     /locus_tag="ACFOF2_001693"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC7107966.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1989..3233)
                     /gene="lysA"
                     /locus_tag="ACFOF2_001694"
     CDS             complement(1989..3233)
                     /gene="lysA"
                     /locus_tag="ACFOF2_001694"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC7107967.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRINPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3244..3414)
                     /locus_tag="ACFOF2_001695"
     CDS             complement(3244..3414)
                     /locus_tag="ACFOF2_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC7107968.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3485..3808
                     /gene="cyaY"
                     /locus_tag="ACFOF2_001696"
     CDS             3485..3808
                     /gene="cyaY"
                     /locus_tag="ACFOF2_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC7107969.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            3841..4260
                     /locus_tag="ACFOF2_001697"
     CDS             3841..4260
                     /locus_tag="ACFOF2_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC7107970.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAELCVNDYIHAVYDFVAQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            4282..4788
                     /gene="luxS"
                     /locus_tag="ACFOF2_001698"
     CDS             4282..4788
                     /gene="luxS"
                     /locus_tag="ACFOF2_001698"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC7107971.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4934..7726
                     /gene="polA"
                     /locus_tag="ACFOF2_001699"
     CDS             4934..7726
                     /gene="polA"
                     /locus_tag="ACFOF2_001699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC7107972.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAADQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         1913 a         2148 c         2185 g         1886 t
ORIGIN      
        1 acaatggaat cgatcgcaaa tctttcccgc tgttcttgaa agaatgcgaa tttcgattta
       61 acttcggcac accgtctcaa cagcttaaaa tcctgcggga ttggtgtggg atttagggct
      121 aatctagtac agcccctaaa atttttcgtt ttcaagcctt caccgcttgc catcggcgtt
      181 aaattttttt acgataagca catagattgt aaacaatcgg tcacaagccg gtttgttttt
      241 tcagaagaca ttatccctgt cagacgctat ttctatatat ttcgcctata acggcttgtt
      301 tttaataaat aattcaagag gtatcaacgt gtctgattcc aaaacgaaag aacgcgccac
      361 attcggcacg cgccgcgcgt ttatgattgc cgccattggg tccgccgtcg gcttgggcaa
      421 tatttggcgt ttcccctata ttgcttttga aaacggcggc ggcgcattca tcctgcccta
      481 tctggtcgcg cttctgacgg cgggcatccc gctgctgctg ctcgattatg ccatcggaca
      541 ccgttaccgg ggttctgcgc ccttggcttt ccgccgcctc ggacgatggt ttgagccggt
      601 cggctggtgg aacgtgatga ccaatatcgt catctgcatc tattacgcgg taattatcgg
      661 ttgggcggca agctatacct attattcggt caacgccgcc tggggtgcgg atccgcaggg
      721 ttttttcttt aaggacttcc tgcaaatggc gggcccggaa gccttgggtt tggattttgt
      781 cggcaaagtc gccggtcctt tggcgggcgt gtgggttttt accgccgcca ttatggcgtt
      841 gggcgtgcaa aagggcgtgg cgcgcgcctc gtcgttcttt atgccgctgc ttttggtgat
      901 gtttttgatt atggtcggca tttcactaac cctgccgggt gcggcaaagg gcttggacgc
      961 attgtttacg cccgactggt cgaaactcgc cgattccaag gtctgggtgg cggcatacgg
     1021 gcagattttc ttttcgcttt ccatctgctt cggcattatg gttacctatt cttcttactt
     1081 gaagaagaaa acggatttgg gcggcacggg cttggtggtc ggttttgcca acagcagctt
     1141 tgaactgctc tcgggcatcg gcgtgtttgc cgcattgggc tttatggcgc aggcgggcgg
     1201 taaggcggtc aacgaggttg cctcaggcgg catcggtttg gcgtttatcg cctttccgac
     1261 cattatcaac caggcaccga tgggctggct gatcggcata ttgtttttcg gttcgctggt
     1321 attcgccggc gttacatcga tgatttccat ccttgaagtg attgtggcgg cgattcagga
     1381 caagctgaac atcgggcgcg tcaacgccac gctgctggtc tgcattccga tgggcattgt
     1441 ttccacgctg ctgttcggta cggcgacggg gctgccggtt ttggacgtga tggacaaatt
     1501 cgtcaacacc tacggcattg ttgccgccgg ctttgtttat gttgccgcca tcatcatcag
     1561 cggcaggctg ccggaattac gcaagcacct gaacgctttg tcctccatcc gcatcggcgg
     1621 cttgtggacg gtctgcgtcg tggttaccgt cgtgatgctc ggctatatgc tgtttaaaga
     1681 taccagcggc ctgatggaga aaaattacga aggttatccg gatggtttcc tcagtatttt
     1741 cggctggggg atgtcggcgg cgttggtcgt gttcgggctg ctgctgtcgt tgctgccttg
     1801 gaaacacggt caggatttca acgtcaaaga cgaacacgaa catgaacaag gagacgaaaa
     1861 atgagtactt ccgccattgt gatgatgatt gtctcaatcg tgataatctg gggagggctg
     1921 ctgctttccc tgttaaggct gccgaacgag taagccttta gagcgttaaa aatgccgtct
     1981 gaaccgcttc agacggcatt ttgatgttcg gcttgcaggc aggcgagttc gtttgccatt
     2041 tgctgttcta aggtttcgcg tcggcggatg agtttgtatc cgccaccgtt aaccaacact
     2101 tccgccgcac ggttgcgcgc gttgtaattg ctcgccatac tggctccgta tgcgcccgcg
     2161 ctgcggataa gcagcaaatc gccttcttcg caggcgatgg tgcggtcttt gccgaggaag
     2221 tcgccggttt cacaaatcgg gccgacgatg ttggcagtca gcggcgcgat gttttttggt
     2281 tctaccgctt cgatgtgatg ataggcatca tatagggcgg ggcgcattaa atcgttcatc
     2341 gccgcatcga ccatcacaaa gtttttctct tcaccgtgtt tgacgaattc gacacgtgtc
     2401 agcagcgaac ctgcgttgcc gaccaagctg cggccgggct caagaatgag tttcagacgg
     2461 cgtgtaccga tcagtttttg aactgcttgg gcatacgcgc ccaaatcagg cactttttcg
     2521 tcttggtaaa caatgccgac gccgccgcct aagtccaaat gttctaaaac aatgccttcg
     2581 gcggcaagag cgtcaaccaa aatcaaaatg cgttcgcagg cttcgaccag cgggcttaag
     2641 tcggtcagtt gcgaaccgat gtggcagtcg atgccgatga ttttcaaatt gggctgttgt
     2701 gcggcgtggc ggtaggcttc gagcgcgtcg gcgtaggcga tgccgaattt gttggctttc
     2761 agacctgtgg agatgtaggg atgggttttt gcatcgacat cggggttgat gcgcagggaa
     2821 acggacgcgg ttttgcccaa acgcgcggca actttttgaa tacggtcgat ttcgggaatg
     2881 ctttccatat tgaagcattt cacgccggca ttcagcgcga actcgatttc tgcctcgctt
     2941 ttgcccacgc ctgaaaatat ggtttttgcc gcatcgccgc ctgccgccaa aacgcgtgcc
     3001 aattcgccgc cggacacaat gtcaaaaccg ctgcccaaag aagcaaagtg tttgatgatg
     3061 ctcaggttgc cattggcttt aaccgcatag cagacgagcg gggagagctc ggcaaaggca
     3121 gtttggtagt gttcaaatgc ttcggtcagc gcggattggc tgtacacata aagcggtgtg
     3181 ccgaatgctt cggcaaggcg ggggtagggg acttgttcgc aaaacagggt catattttcg
     3241 ttttcatttt tgggtttgtg gagcggattg cggtttgctt tgaagctgca aaccggtttg
     3301 gattacgccg aaacgcgcct tgtcgccttc tttgggcagg tagaggtcgc ctttgtaacc
     3361 gcaggccgag agcaggaggg cggttgccgc cgcaaaaaat acgccgtatt tcatcggtaa
     3421 acttccttca taagcgcgaa tgtggcaaga ttcggtatct taaacaaaaa acacgcaaaa
     3481 agctatgatg accgaaagcg agtttatccg cgcgagcgaa gcattatttg aacacatcga
     3541 agaccaaatc gacgaaaacg gctgggattt cgactgccgg tttgccggaa acgtcctgac
     3601 catcgaagcc ggagacggca cgcaaatcat cgtcaaccgc cacacgccca atcaggaatt
     3661 gtggattgcc gcaaaaagcg gcggctacca tttcgccgag caaaacggca aatggctggc
     3721 aacgcgcgac ggacgcgatt tttacgacgt tttaaacgaa gccctgagcg cggcttcggg
     3781 cgaagcggtt gagattgccg aattgtaatt tgagagtgat cacaaagaga gcgttaccca
     3841 atggcacaat taccgctata tctgacttct gaaatcaaag actttactgt cggcacgcct
     3901 aaagttttag aatcattttc caaacatatc ccttatggtg tcgtctttga agacgacggc
     3961 gacacaggct acttctatgc cgcttcgcaa gacgggattt tagatgcctt gcacatctat
     4021 aatgtcgaag atgtatccga caaacatatc cccaatcatg tcttgatttt atgggatgat
     4081 gcctgcacca tagccgaatt gtgtgtcaac gactacattc atgccgtcta tgattttgtc
     4141 gcgcaggcag gatattgccg caacggcttc cctgaagcag gcggcgaatg ggtgaaagtc
     4201 gaaaaccgcg tcttggacga tgaattgctg gacaaaatcc tatcccgaaa atctacataa
     4261 cctactcaaa aggataacaa aatgccccta ctagacagtt tcaaagtcga tcacacccgt
     4321 atgcatgccc ccgccgtacg cgtggcgaaa accatgacca cgcccaaagg cgacaccatt
     4381 accgtgttcg acctgcgctt ttgcgttccc aacaaagaaa tcctgcctga aaaaggcatc
     4441 cacacgctgg agcatttgtt tgcaggcttt atgcgcgacc acttgaacgg aaacggcgtg
     4501 gaaatcatcg acatttcccc gatgggctgc cgcaccggtt tttatatgag tttgattggt
     4561 acgccttccg aacagcaggt tgccgatgcg tggctcgctt cgatgcagga tgttttgaat
     4621 gtcaaagacc aaagcaaaat ccccgagttg aacgaatacc aatgcggcac ttatcaaatg
     4681 cactcgctcg ccgaagcgca gcaaatcgcg caaaacgtgt tggcgcgcaa agtggcggtg
     4741 aacaaaaacg aagagctgac gctggatgaa gggctgctga acgcctaatc cgccaaaaat
     4801 gccgtctgaa caagggtttc agacggcatt tgccttttcc gttataatcc ggggttgtgc
     4861 gggggcgggt tttaagccgg catcgtcctt ccctattttt ttctgtccct tatcggtttt
     4921 aagcgggttt tttatgtcca acagacctac actcctcctc gttgacggct cttcctatct
     4981 ctaccgtgcg tatcacgcga tggggcaaaa cctgaccgca cccgacggcg cgccgacggg
     5041 cgcgctgtat ggcgtattga atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta
     5101 ttgcgcggtg gtttttgatg cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata
     5161 caaggcgacg cgcccgccga tgcccgacga tttgcgcccg caggcggaag cactgccgga
     5221 tttagtgcgc ctgacgggct ggccggtatt ggtgattggg caagtggagg cggacgatgt
     5281 gatcggcacg ctggcgaaac agggggcgga acatggtttg cgagtcattg tttcgaccgg
     5341 cgataaggac atggcgcagt tggtggatga gcgcgttacg ctggtgaaca cgatgagcgg
     5401 agaaacgctg gatattgaag gcgtgaaggc aaaattcggc gtgcgccccg accaaatccg
     5461 cgattatctc gcgctgatgg gcgataaggt ggacaacgtg ccgggcgtgg aaaaatgcgg
     5521 cccgaaaacg gcggtgaagt ggctggaagc ctacggctcg ttgcaaggcg tgatagaaca
     5581 cgcttcggaa atcaagggca aagtgggcga aaacctgcaa gccgcgctgc cccaactgcc
     5641 gctgtcgtat gatttggtca cgattaaaac cgatgtggac ttgcacgccg agctttcaga
     5701 cggcatcgaa agcctgcgcc gtaccgcgcc gaaatgggcg caactggttg tcgatttcaa
     5761 acgctggggc ttccgcacct ggctgaaaga agcggaatca aacatgaata ccggctcgac
     5821 cgatgatttg ttcggcagcg acagcatcgg cgagcaggcg gctttgaatg cggaaatgcc
     5881 gtttgaaaaa caagccgaaa aagccaccgc ccccgaaaaa ctggattatc aagccgttac
     5941 caccgaagcg cagtttgccg ctttgttgga caaactgtcg cgggcggaca caatcggcat
     6001 cgatacggaa accacgtcat tagacgcgat gaacgcctcg ctggtcggca tcagcatcgc
     6061 tttccaagca ggcgaagcgg tttacatccc cgtaggacac agcctgaccg ccgcgcccga
     6121 acagcttgat ttgcaagacg tattaggccg tctgaaaccg catttggaaa accccgccct
     6181 gaagaaaatc gggcaaaacc tcaaatacga ccaacacgtt ttcgctaact acggcatcgc
     6241 cctgaacggc attgccggcg acgctatgct cgcttcctac atcatcgaaa gccatcttgg
     6301 acacggcttg gacgaattgt ccgaacgttg gctgggcttg gaaaccatta cctacgaatc
     6361 attgtgcggt aaaggcgcga agcaaatcag ttttgccgat gtcgccatcg ggcaggcgac
     6421 cgaatacgcc gcccaagacg ccgatttcgc cctgcgcctc gaagcgcacc tgcgcgcgca
     6481 aatggacgaa aaacagcttg aaatgtatga aaaaatggag ctgcccgtcg cgcaggtatt
     6541 gtttgaaatg gaacgcaacg gcgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc
     6601 ggaactcggc gccgagctga tgaagctcga acaacaagcc tatgccgccg cagaccagcc
     6661 gttcaacctc aattcgccca aacagctgca agaaatcctg ttcgacaaaa tgggcatccc
     6721 caccaaaggc ctgaaaaaaa ccgccaaagg cggcatttcc accaacgaag ccgtgctcga
     6781 acagctcgcg cccgactacc ccctgcctaa aatcatcctg caaaaccgca gcctggcgaa
     6841 gctcaaatcc acctacaccg acaaactacc cgaaatgatt tcccccaagg acggccgcgt
     6901 gcataccacc tacgcccaag ccgtcgccat taccggccgc ctcgccagca acaaccccaa
     6961 cctgcaaaac atccccatcc gcaccgccga aggccgccgc gtgcgccgcg cctttaccgc
     7021 accgcaaggc agcgtcatcg tttccgccga ctattcccaa atcgagctgc gcattatggc
     7081 gcacctctcc ggcgacaaaa ccctgattgc cgcgttccaa aacggcgaag acgtacaccg
     7141 ccgcaccgcc gccgaagtgt tcggcactgc gcccgaaaac gtctcgtccg agcaacgccg
     7201 ctacgccaaa accatcaact tcggcttaat ttacggtatg gggcaatacg gtttggcaaa
     7261 atcattgggc atcgacaacc tttccgccaa aaactttatc gaccgctact tcgcccgcta
     7321 ccccggcgtc gccgaataca tgcagcgcac caaagaacaa gccgccgccc aaggctacgt
     7381 cgaaaccctg ttcggcagaa ggctctattt gcccgacatc cgcaacaaaa acgccaacgc
     7441 ccgcgcagga gccgagcgcg ccgccatcaa tgcccccatg caaggcaccg cgtccgacct
     7501 catcaaacgc gccatgatag acgtgcgaaa ctgtctttca gacggcatcg gcagcaaact
     7561 ggtcatgcag gtgcatgacg aactggtgct ggaagtcgtt gaaaccgaac tggattttgt
     7621 caaagaaaaa ctgccgcaga ttatggcgaa agtggacggc ggattattgg atgtaccgct
     7681 ggtggctgag gttggcgtag gggagaattg ggaagaggca cattgattga aaggtgttat
     7741 atgctatctt tatttaaata aaatttaatt tttggtatat tttttctaaa tgttcctata
     7801 gtatagtgga tcaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
     7861 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
     7921 aggcaacgcc gtactggttt ttgttaatcc actatattcc gccatctcta agatttacag
     7981 cgatacacgg gtgatttaag gaatgcccga accgtcattc ccgcaacttt tcgtcattcc
     8041 cacgaaagtg ggaatctaga aataaaaagc agcaggaatt tatcggaaat aactgaaacc
     8101 gaacagacta gattcccgcc tgcgtgggaa tg
//
LOCUS       DAXIHS010000082         8131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXIHS010000082 DAXIHS010000000
VERSION     DAXIHS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.082"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(70..2106)
                     /locus_tag="ACFOF2_001700"
     CDS             complement(70..2106)
                     /locus_tag="ACFOF2_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC7107973.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     DKEARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRGAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2194..3066)
                     /locus_tag="ACFOF2_001701"
     CDS             complement(2194..3066)
                     /locus_tag="ACFOF2_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC7107974.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3336..5726
                     /gene="gyrB"
                     /locus_tag="ACFOF2_001702"
     CDS             3336..5726
                     /gene="gyrB"
                     /locus_tag="ACFOF2_001702"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC7107975.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            5823..7208
                     /locus_tag="ACFOF2_001703"
     CDS             5823..7208
                     /locus_tag="ACFOF2_001703"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC7107976.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVADVMEGCIK
                     RGLAADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTALTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKSTYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7279..7377)
                     /locus_tag="ACFOF2_001704"
     CDS             complement(7279..7377)
                     /locus_tag="ACFOF2_001704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC7107977.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(7950..8026)
                     /locus_tag="ACFOF2_001705"
     tRNA            complement(7950..8026)
                     /locus_tag="ACFOF2_001705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7990..7992),aa:Asp,seq:gtc)
BASE COUNT         1885 a         2249 c         2268 g         1729 t
ORIGIN      
        1 ccgtgcaaat gctgatgccg tctgaaacac tgtttgattg tttcagacgg cattttttgt
       61 tcacatcgat caagccgcgt tgtcgaaacc gctgtggcgc aagagtgcgt ctatgcgcgg
      121 ttcgcgtccg cggaaggctt tgaaggattc tgccgcgccg cgcgatccgc cgacggcgag
      181 gatttcctgc caaaagcgtt tgcctgtggc ggcgacatcg tcgctttctt caaaggcggc
      241 gtatgcgtcc gcactcaata cttccgccca cgcgtagctg taatagcctg cggaatagcc
      301 gcctgcgaag atgtggccga agctcaaggc gaagcggttg tattcgggcg gttggatgac
      361 ggcgactttt ttgcgcacgc tgtctaaaac ctgttgccag tttttcagac ggccttcgtc
      421 gtcttcgctg taaatcatca tatcgaagag ggcaaactcc atttggcgga cgaggaacat
      481 accgcgttgg aagtttttgg cggcgagcat tttgtcgaag agttctttcg gcaggggaac
      541 gccggtttct tcgtgggctg acatttgtgc caagacgtcg tattcccaga cgaaattttc
      601 cataaactgg ctgggcagtt cgaccgcgtc ccattctacg ccgttgatgc cggatacgcc
      661 cagttcgtcc acttgggtaa gcaggtggtg cagcccgtgt ccggtttcgt ggaagaggat
      721 gaggatttcg tcgtggctca agcgggcttc tttgtcgccg acgggcgggg cgaagttgca
      781 gacgaggtag gcggtgggca gttgcagcgt gccgtcggcg aagcggcggc ggcctttgta
      841 gtcgttcatc cacgcgccgc cgcgtttgcc ttcgcgtgcg tacaaatcca tataaacgcc
      901 gcctatggtt tcgccgtttt gttgcaattc aaaatagcgc acgtctttgt gccagacggg
      961 gacggttttt tcggtaaatc cgatgccgta gagttttttg atttgggcga acagtccgtt
     1021 taatactttg ccgacgggga agtatttttt gacttcggtt tcgctgaacg cgtatttggc
     1081 ttcgcgcagt ttttcgctgg cgtagcccaa gtcccacggt tgcaaatcgg cgaggttcag
     1141 gctttcgcgg gcgaaggctt tgacttcggc gaggtctttt tcggcgtagg gtttggcgcg
     1201 gcgggcgagg tcgtgcagga agtttaaaac ttgttcgggc gtgtccgcca ttttggttgc
     1261 cagcgacaat tcggcgtagt ttttgaagcc gagcagtttg gcggtttgca gggcgtttgc
     1321 gagcgtgcgg tcgatgttgg cggtgttgtc gaatttgccg tcgtctgaaa gttcgctggc
     1381 gcgggtaacg taggcgcggt agatttgttc gcgcagttcg cggttgtcgg cgtattggat
     1441 gacggcgagg tagtgcggaa tctgcaaacc gattttgtag cctgttttgc cttcgctttg
     1501 cgcggcggcg gcaaacatgg cgagcgcgtc ttcgggaatg ccggcaagcg gtgcggcatc
     1561 gtcaaagtaa atgccgaacg cgtcggtcgc gtctaggacg ttttgggaga atttggcgga
     1621 aagttgcgcg ccttcggttt gcagttttgc cagttctgcc tgctgttcgg gcggcagttc
     1681 cgcgccgctg aggacgaaat cgcgcagatc gtggttgagt ttggtttttt gcgcggggga
     1741 gagggtgtcg aactcggggg agtttttgat ggttttgaag cggttgtaca gctcgatgtc
     1801 ttgtccgatt tcggtgaaga agacggtgat ttcgggcatc agttcgttat agacggcgcg
     1861 cagttcgggc gtgtcggcga cggagttgag gtgcgacacc acgccccaaa tcctgccgac
     1921 gcgctcggtg atgccggtca gacgctcgac ggtgttcgcc cagccggtgt gcgtttgggc
     1981 tttgatggcg gcgatttgtt cgcgcgcttc ggcaatggcg gtttgcaggg cgggtttgat
     2041 gtcttcggtt ttgatttgat caaaacgggg ttcttcgccc aaatggagca gtgcgttgtc
     2101 agtcataaga tgggtttcct ttgcgtgttg tgcttcagac agcatatcgg caatatgccg
     2161 tctgaagccg gaaatggggt cggtcgggga aagttaaagt gtttcggagc cggcggggta
     2221 atttaacagg cggtattgta cgcgcaaaaa ttcgtccata accgcctgtt ggatttccag
     2281 ccgctttgaa acgggggagg cgaagcggac gatgatgcgg tatgccttgt catcgtacgg
     2341 cacgcgggta acgcgcggtc tggcggcggg cgtgataaac agtttttccg cctgcacgtt
     2401 ttccaaatgc cgttggatgg cggggatgta gggcgcgcac aagggctcga gtacggcttt
     2461 cagacggcat acggcttcat ccgaatccaa atggatgggg acggggattt cgaccgtatg
     2521 gatgacgtag tcgcccaaaa tattgtcgcg gcgcacggga tggctcaaca acaggctgtt
     2581 ggggaaagaa acggtggttc ccgcaagctg tccgaccagg gggttcggac cgacctgcat
     2641 catcagcgtg ttcaacaggt tgatgtcgac cacgcgcccg cgcaggccgt tgatttcgat
     2701 atagtcgccg accgagtatt gctgggtggc agaccttaaa atactgcccg acagacacat
     2761 aatcagttcc tttgtcgcca cgacgaccgc cgccgccacc gcaaacatcg acaaagccag
     2821 cgtttggatt tgcgccgacc agataaatgc cagcgaaaac agcaccaaaa gcagcgttat
     2881 attgcggctg gcaaccaaaa accgccgctt gctttcgatg ccgaaatccg gatgccgttt
     2941 gaagtggata ttcaacagaa gggcgcgcgc cagcagcaaa gccgcgacca ccgccacgga
     3001 ttcgaccgcc tccgcacgta tcgggacggc accgagccaa gtgtccaaca tattccatat
     3061 ttccatttcc gcgcccctgt ccgaaaagta tagggtgtag attttagtgg caaaaaaacg
     3121 cttttgccac tttacgggac aatccccgac ctgatgccgg aggcaaacgt accgtttcat
     3181 cttttcccgg cggcacactg atgcggataa cgctgaatta ggcggcaaaa tcggctataa
     3241 tacacaatta tatatgccgt cccgcccgct ttttctacaa ggtgcggact tgttttggcg
     3301 gcatcgcaaa atcttttcaa aatccggcaa aaaatatgac tgaacaaaaa cacgaagaat
     3361 acggcgccga cagcattcag gtgctcgaag gcttggaagc ggtacgcaaa cgccccggta
     3421 tgtacatcgg cgacacgcag gacggcagcg gcctgcacca catggtgttc gaagtattgg
     3481 acaacgccat tgacgaagca ctcgccggac attgcgacaa aatcaccgtt accatccacg
     3541 ccgaccattc cgtcagcgtc gccgacaacg ggcgcggtat gcccaccggc atccacccga
     3601 aagaaggacg ctccgccgcc gaagtcatca tgaccgtatt gcacgcgggc ggtaaattcg
     3661 acaacaacag ctacaaaatc tccggcggcc tgcacggcgt gggcgtgtcc gtcgtcaacg
     3721 cgctgtccga ctgggtaacg ctgaccatct accgcgacgg caaagaacac ttcgtccgct
     3781 tcgtgcgcgg cgaaaccgaa gagccgttga aggttgtcgg cgattccgat aaaaaaggca
     3841 cgaccgtgcg cttcctcgcc agtgcggaaa ccttcggcaa cgtcgaatac agcttcgaca
     3901 tcctcgccaa acgcatccgc gaactttcgt tcctgaacaa cggcgtggac atcgaattga
     3961 ccgacgagcg cgacggcaaa cacgaaagct tcgccctttc cggcggcgtg gctggattcg
     4021 tgcaatacat gaaccgcaaa aaaacgccct tgcacgaaaa aatcttctac gcgttcggcg
     4081 agaaagacgg catgagcgtc gaatgcgcaa tgcaatggaa cgacagctat caggaaagcg
     4141 tgcagtgctt caccaacaac atccctcagc gcgacggcgg tacgcacctg accgcgttgc
     4201 gccaagtgat gacgcgcacc atcaacagct acatcgaagc caacgaagtc gccaaaaaag
     4261 ccaaagtgga aaccgccggc gacgatatgc gcgaaggttt gacctgcgtg ttgtccgtca
     4321 aactgcccga ccccaaattt tcgtcccaaa ccaaagacaa actggtttcc ggcgaaatcg
     4381 gtcccgtcgt caacgaagtc atcagccaag ccctgaccga cttcctcgaa gaaaaccctg
     4441 ccgaagccaa aatcatcacc ggcaaaatcg tcgatgccgc ccgcgcgcgc gaagccgccc
     4501 gcaaagcccg cgaaatcacc cgccgcaaag gcgtaatgga cggcttgggg ctacccggta
     4561 aactcgccga ctgccaagaa aaagaccccg cattgtcaga actttatctg gtcgagggcg
     4621 attctgcagg cggttccgcc atgcaggggc gcgaccgcaa attccaagcg attttgccgc
     4681 ttaagggcaa gattttgaac gtcgaaaaag cgcgctttga aaaaatgctc gccagtcagg
     4741 aagtagccgc tttgattacc gcgttgggcg cgggcatcgg caaagaagaa ttcaatgccg
     4801 agaagctgcg ttaccaccgc atcattatca tgaccgatgc cgatgtggac ggcgcgcata
     4861 tccgcacctt gcttttgacc ttcttctacc gccaaatgcc cgaactggtc gagcgcggct
     4921 acatctatat cgcccagccg cctttgtata aagcgaaata cggcaaacag gaacgttacc
     4981 tcaaggacga gttggaaaaa gaccaatggc tgctcggtct tgccttggaa aaagccaaaa
     5041 tcatttcaga cggccgcacc atcgaaggcg cagaacttgc cgacaccgcc aaacaattcc
     5101 tgttggcaaa aaccgtcatc gaacaggaaa gccgcttcgt agacgaactc gtcctgcgcg
     5161 ccatgctaca cgcgtcgccc attgatttga cgtcgtctga aaacgccgat aaagccgttg
     5221 ccgaactttc cggtctgctt gacgaaaaag aagtcgccct cgaacgcatc gaaggtcatg
     5281 aaggacaccg gttcatcaaa atcacgcgca agctgcacgg caacgtcatg gtcagctaca
     5341 tcgaacccaa gttcctcaac agcaaagcct accaaaccct cacccaaacc gccgccgcgc
     5401 tcaaaggcat ggtcggcgag ggtgccaagc tttacaaagg cgaaaacggg tacgacgcgg
     5461 acagctttga aaccgctttg gacatcttga tgagcgttgc ccaaaaaggt atgtccatcc
     5521 aacgatacaa aggcttgggc gagatgaacc ccgagcagct gtgggaaacc acgatggatc
     5581 ccgccgtgcg ccgcctgttg aaagtgcgca tcgaagatgc cattgccgcc gacgaagtgt
     5641 tcgttacgct gatgggcgac gaggtcgagc cgcgccgtgc ctttatcgaa aacaacgcgc
     5701 tgattgccca aaatatcgac gcataagtgc cgttttaaaa aaggagacgg gcatcgtgcc
     5761 gcgtctcctt tttggttggt caaacggaac ctgtgccgtc tgaaaaaccg tcggagcaaa
     5821 atatgatcag cattttcgat attttcaaaa tcggtatcgg gccttccagt tcgcatacgg
     5881 tcggcccgat gaaggcagcc gccgcctttg cggcaggttt ggatgcacag gctgttcgca
     5941 tcgtcatcga catttacggc tcgctcgcac tgaccggata cggacacggt acatttgacg
     6001 cgctgatgct cggtttggaa ggcagcctgc cgcacgacat cccgcttgcc ggcattcccg
     6061 aacgcctcga acgcatccgc acgcagcaca tcctccggct caacgggcaa gaaatccgct
     6121 tcatccccga ccgcgacctg aacatactcg gcaatcaagt gctgcccaaa caccccaaca
     6181 gcctgcgttt taccgcctat gcttcagacg gcacggtatt gaatgaacag gtttattatt
     6241 cggtcggcgg cggctttgtc gttaccgaag aagattttga ccggcaggcg gaaacggaaa
     6301 aagccgttcc ctatccctat accagttgcg ccgaactgct tgcccgatgc cgtctgaacc
     6361 ggctcgacat ctccgaagtc gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag
     6421 ccgaaatccg ccgccgcgtt gccggcgttg ccgatgtgat ggaaggctgc atcaaacgcg
     6481 gtttggcggc agacggcgag ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc
     6541 ttgccgccaa gctcaaagtc ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga
     6601 tggtgtacgc catggcggtc aacgaagaaa acgccgccgg cggacgcgtc gttaccgcac
     6661 tgaccaacgg cgcggcaggc atcattcccg ccgtattgca ctatttccgc aagttcaatc
     6721 cgcacgccac acaggaacgc gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc
     6781 tctacaagac caacgcctcc atttccggtg cggatgtcgg ctgtcagggc gaagtcggcg
     6841 tagcgtgttc gatggcggcg ggcgcatacg ccgaagtcat cggcggcacg cccaaacaag
     6901 tggaaaacgc cgccgaaatg gcgatggaac accatttggg gctgacttgc gaccccgtcg
     6961 gcggactggt gcaaatcccc tgcatcgagc gcaacggcat cgccgccgaa aaagccctca
     7021 aactcggcac gctcgcgctt ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag
     7081 tcatccgaac catgttacag acagggcgcg acatgaagtc cacctacaaa gaaacctcgc
     7141 ttgccggact cgccgccacg ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg
     7201 agtgctgagg atggacagaa acgaaaatgc cgtctgaaaa cagtttttta gacggcattt
     7261 catttatttc ttgcggcttt accgtttcag tgccagcgtc aacacgcccg ccgtaaccag
     7321 ccccaagcct atccattcct gcgtgttcgg gcgttcgtct aagaaaacca ccgccatcag
     7381 gaatgcaaac cggcgttgtt tgcagcatcc ggcaagaatt tttatagtgg attaacaaaa
     7441 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggaaccga ttcacttggt
     7501 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
     7561 tttgttaatc cactataaaa ggtcttttct aaaatatgca gatattcgaa tatcgaattt
     7621 gccttatgat tacggttttc tgtcttatct gccttaaaac gccggtattc cgtttgaacc
     7681 aaattatatt tgccgaaaca ctcaaaaatc tcccttacct gcccgacaga catcaagcct
     7741 tcgttgttgt agctgagaaa aatatattga aagtccgcat ccctaatcaa agcctcaaat
     7801 accggaacca catccgattt tgagcaagtc cgcgctaaaa cacatcgtac agcgcaaaac
     7861 cggatgggaa tggcaaaatc cggaaacaaa catttttcaa agcatacaaa caaaaaaata
     7921 ccaaccggag gttggtattc cttaactttt ggcgcggcgg acggggctcg aacccgcgac
     7981 ccccggcgtg acaggccggt actctaacca actgagctac caccgcgcat ccattgtttg
     8041 caacaatgaa agaaaacttg gtgggtgatg acggagtcga accgccgaca ttctgcttgt
     8101 aaggcagacg ctctaccaac tgagctaatc a
//
LOCUS       DAXIHS010000083         7924 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXIHS010000083 DAXIHS010000000
VERSION     DAXIHS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.083"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(238..993)
                     /locus_tag="ACFOF2_001706"
     CDS             complement(238..993)
                     /locus_tag="ACFOF2_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC7107978.1"
                     /translation="MKYIRLFFLCTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1173..2345)
                     /gene="nirK"
                     /locus_tag="ACFOF2_001707"
     CDS             complement(1173..2345)
                     /gene="nirK"
                     /locus_tag="ACFOF2_001707"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC7107979.1"
                     /translation="MKRQALAAIIASMFALAACGGEQAAQAPAETPAASAEAASSATQ
                     AAAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETIEKTMKMDDGVEYHYWT
                     FNGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGPGGGAAASFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIIPAGGSAMVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2719..4974
                     /locus_tag="ACFOF2_001708"
     CDS             2719..4974
                     /locus_tag="ACFOF2_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC7107980.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPNETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFI
                     FYNMGFVRRSTATASTLAAGAIFMLGGIPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFSRDK"
     gene            5153..5686
                     /locus_tag="ACFOF2_001709"
     CDS             5153..5686
                     /locus_tag="ACFOF2_001709"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC7107981.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5749..6933)
                     /locus_tag="ACFOF2_001710"
     CDS             complement(5749..6933)
                     /locus_tag="ACFOF2_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC7107982.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLNASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6973..7377)
                     /locus_tag="ACFOF2_001711"
     CDS             complement(6973..7377)
                     /locus_tag="ACFOF2_001711"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC7107983.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1839 a         1842 c         2177 g         2066 t
ORIGIN      
        1 ggatgtaggt tcgtgggaat gacgaaaagt tgcgggaatg acgaaaagtt gcgggaatga
       61 cggaaagttg cgggaatgac ggaaagtggc gggaatgacg gttcgggcat tccttaaatt
      121 acccgtgtat cgctgtaaat cttagagatg gcggaatata gtggattaac aaaaaccagt
      181 acggcaaggc gaggcaacgc cgtactggtt tttgttaatc cactataatt gaaggggtta
      241 tcggcttgtg caacggaagc ccaagttgtg caagacatat ttggattgca ggctggtacg
      301 gatgccgtag cggaggaagg cggcatagtt ggacgagtcg ctcgacccga tagacgcgcc
      361 gctgcaaaac atttgcgcgt tggcattgcc ggaagaaagc aggctgctgt tgaaatcttc
      421 cgtccattcc caaatcagcc cgtgcatatc ataaacgccc cagtagttcg ggcggccttt
      481 gccgacatcg tgcaggcctt tccgtccgcc gtcggcatac caatcgagaa tagtgcggtt
      541 gtagccgggt tcgtttgagc cgtttttctg cgtggcggaa gcaagtccgg caaattccca
      601 ttcgtcaatg gtcggcaggc gtttgccttg tgcggcgcaa taggcgttgg cggcaaacca
      661 ggaaacattg gttaccggtt gttttaattc gcccgccttc ggcgcatagc tgcggctgcc
      721 gtttttcatc caatgcttca ggtaagcggg ttctgcctgt ttggaaccga tcctgccttt
      781 ttgccattgg gggtggctgt tgacaaattc ggcaaactcg gcattggtaa cgggatattt
      841 atccagtttg aacggtttga ctttaatcag gccggtatct tttttcagat aaagcgggcg
      901 gtagctgccg ccttcgattt gaaccatttc ggcagccgcc gcttgagtgc cggcgagtgc
      961 cgtacagaga aaaaataacc ggatgtactt catatcaagc ctcctgacag acggttaaaa
     1021 ttgatttttc agacagtatt ttaaacgaaa gctttgaatg ccgtctgaag aatcaatctt
     1081 tcgaagtaaa aaaattgatt atgaaaaaac cactgccgtg cgtaattaag tacggcggca
     1141 gtggttcgtt tgcctaaaaa cgggtcttca atttagtaat cgctttttgc gggggcagag
     1201 gctgcaggag cagaagctgc gggagcggaa gcagcaggag ctgcaccgtt accggcataa
     1261 gcagtatcac tcaatttttg agtcatgatt tcagggtttt ctgcaccttc tactttcaat
     1321 tgacccagtg cgcctttgtt gaatgcacgg aagatagagt ggtcaaccaa ggtgtagctg
     1381 cccgggatgt cgactttgaa ttcaaccata gcagaaccac ctgcaggaat gatggtgctt
     1441 tgtacgtttt cgttaatcag tttaccgcct tcaacataaa ctttgtcgaa gatttcgccg
     1501 attacgtgga aggaagatac caagttagga ccaccgttac caacgtacat acgtacagtt
     1561 tcgcctgctt tggctttcag ggcgttgtcg ccggcgatag cgcctacgtg accgttaaat
     1621 acaacgtatt caggttgttc ggcgacggct ttatccatat cgaacggttg caggccttgt
     1681 gcaccttttt tacctttggt gtagaagtcg ccttgaacga tgtagaattc tttatccact
     1741 ttcggcagac cttctttagg ttcaaccaaa atcagaccgt acataccgtt ggcgatgtgc
     1801 ataccgacgg gcgcaacggc gcagtggtag atatacagac cgggttgcag ggctttgaag
     1861 ctgaatgtag aggtacggcc cggggcagtg aaggaggctg ccgcgccgcc gccaggaccg
     1921 gtagctgcgt ggaagtcaac gttatgagga acggtagaag aaggattgtt agagaattct
     1981 acttctactg tatcaccttc gcgtacgcga atcatgcggc ccggaacatc gccgttaaat
     2041 gtccagtagt ggtactcgac gccatcatcc attttcatgg ttttttcgat ggtttccatt
     2101 ttcacgcgaa ctttggcagg gtagtcgcgg tcgattgcag gaggtacttc gggagcgtgg
     2161 gtggtaaccg catcgataac gggcagttcg cctgccggag tttcggcggc agcttgtgta
     2221 gcggagcttg cagcttcagc ggaagcggca ggggtttcag caggggcttg agcagcttgt
     2281 tcgccaccgc aggcggctaa agcaaacatg gaagcgataa ttgcggctaa tgcttggcgt
     2341 ttcataatgt tttccttttg taagaaaagt agggagattg gttaagaact ggcgtgatta
     2401 tgcgtttgcc ccattacagg tattttgatt taagtcaaat aattcataaa atatgaactt
     2461 aaaatgatac ttctaatact ttataactac taaaaattat aataaataag ctgatttgct
     2521 taagggcttc ggcaaatcag gcaaaggcag ggtagttgcc gactaaagaa taatgatagt
     2581 cattatcatg tatgtatttg ttttatatgt gagtttgttt gaacaaatgt ttatttgtgc
     2641 ggtaaaccgt gctacaatct ttaacattca tattctgtga attttaaaca accactttta
     2701 ccaaaaggag ctctaaaaat gggacagtac aagaagctat ggtacttgct gtttgccgtt
     2761 ctggcagtat gctttaccat tcttggctat atgggcagcg aggtttataa aaaagccccg
     2821 ccttaccctg aacaggtcgt ttctgcatca ggcaaggttc tgatgacgaa agacgatatt
     2881 ttggcaggtc agtctgcgtg gcaaagtacc ggcggtatgg aggtcggttc gattctgggt
     2941 catggtgcat atcaggctcc ggactggact gcggactggc tgcatcgtga gttggttgca
     3001 tggttggatt tgactgcgca acagacttac ggcaaaaaat tcgatgaggt ttcccctgag
     3061 gaacaagctg tcttgaaaac acgcctggct gatgaatacc gtaatcaaag ccgtgtgaaa
     3121 gaagatggtt ccgttgtcat cagcgatacg cgtgtaaaag ctatcgaaag cattctgcct
     3181 tactaccacg gtgtttatgg cgatgatcct aagctgcaaa ccactcgtga acactttgca
     3241 atgaaaaaca atacattgcc aagtcaagag gcacgtgaaa aactgttcaa cttcttcttc
     3301 tggacttcat ggtctgcttc gaccaaccgt cctaatgaaa cctttaccta taccaacaac
     3361 tggccgcacg agcctttgat caacaatgtg ccgaccactg aaaactatat gtggtcattc
     3421 accagcgttg ttcttctgct gatgggcatc ggtttgctga tgtggggtta ttccttcctg
     3481 accaaacacg aagaagtgga agtgccgtct gaagacccga tttccaaaat ccaactgacc
     3541 ccttcgcaaa aagcattggg caaatacgtc ttcctgacgg tcgccctgtt tgtggtacag
     3601 gtattgctgg gcggtctgac cgcgcactac accgtcgaag gccagggttt ctacggcatt
     3661 gacgaggcgt tgggctttga aatgtccgac tggttcccct acgccctgac ccgtacttgg
     3721 cacatccaat ccgccatctt ttggattgca accggctttt tgacggcagg cttgttcctc
     3781 gcgccgattg tcaacggcgg caaagatccc aagttccaac gtgccggcgt aaacttcctc
     3841 tatatcgccc tcttcatcgt ggtcggcggt tcttacgcgg gcaacttctt cgcgttgacg
     3901 cacatccttc ccccggaatt taacttctgg ttcggacacc aaggttacga atacctcgat
     3961 ttgggacgtt tctggcaact tctgctgatg gtcggtctgt tgttgtggct gttcctgatg
     4021 ctgcgttgca cgatttccgc ctttaaagaa aaaggcgtgg ataaaaacct gctggcaatc
     4081 tttgttgctt ccatggttgg tgtcggtgtg ttctacgcac cgggcctgtt ctatggcgaa
     4141 aaatctccga ttgccgtaat ggaatactgg cgctggtggg tggttcacct gtgggtggaa
     4201 ggtttcttcg aagtgttcgc taccgctgcc ttcgcattta tcttctacaa catgggcttt
     4261 gtccgccgta gtaccgctac tgcttctacc ctggctgcag gtgctatctt catgctgggt
     4321 ggtatcccgg gtacgttgca ccacttgtat ttctccggct ctacttctgc ttctatggca
     4381 attggtgcct gcttctccgc tttggaggta gtaccgctgg tattgctggg ccgtgaagca
     4441 tacgaacact ggtcttacca acacttgtcc gattgggcga aacgtttgcg ttggccgctg
     4501 atgtgcttcg ttgcagtcgc cttctggaac atgattggtg ccggtgtatt cggtttcctg
     4561 attaacccgc cgatttccct gttctatatc caaggtttga acacttccgc cgttcacgcg
     4621 cacgccgcct tgttcggtgt gtacggtttc ttggcattgg gcttcgtatt gctggttgcc
     4681 cgttatttga aaccgaacgc gcagtttgac gacaaactga tgacttgggg cttctggttg
     4741 ctcaatggcg gtttggtcgg tatgattgcc atcagcctgc tgcctgtcgg cgcgattcag
     4801 gcatacgcct ccatcacgca cggtctgtgg tatgcccgca gcgaagagtt cctgcaaatg
     4861 gaaatcctcg acaccctgcg ctgggtacgt acggcagctg acttgatctt tatcggtggt
     4921 gcaatctgtg tcgccatcca agccactaaa atcgtattca gccgagacaa ataagttttg
     4981 cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt tttttattgc ggattgggac
     5041 gggaatggaa aaggatgtgg attttttatt ttaatcggct atacaattgc tgctttcatg
     5101 ccgtctgaag cacgtttcag atggcatcgg tttacatcag ataaggataa aaatgggtat
     5161 ttacgatttt caaatgaaag atgcggaagg caatgcggtt gatttgtcag gctatcgcgg
     5221 caaggttctg ctgattgtca acacggcaac gcgttgcggt ctgaccccgc aatacgaggc
     5281 tttgcagaag ctgtatgcac aatataccgc agaaggcttg gagattttgg attttccgtg
     5341 caaccagttc cgcgaacagg ctcccgaaag cagcggagaa attgcccaag tgtgtatgat
     5401 gaagtttggc acgaagttta agattttcga caaaatcgaa gtcaatggag caaataccgc
     5461 gcccttgtat gcctacctga aatccgtcaa accgcaagat aaaggcaatc atctgtttaa
     5521 agatttcgtg ttgaagctcg cagctttggg agagaagcgc gacgaaggcg acatcaaatg
     5581 gaactttacc aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt ttgcgcctag
     5641 tgttacgccc gaagaaatcg aagccgatat tcgggcattg ctgtaatctg ccgccgtctt
     5701 gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt attccgaaat
     5761 atcggcaaca ccgctttcgg ggaagatggg caaatccggc ggtgggagca gggtatccaa
     5821 agtcagggcg gacgcattga gtgtcgggta gccttcaaac gcgaccgaat aaccgctgtt
     5881 tccgttcata cggattcggg cgtgcgcgcc tagagggaaa gtgattttgt cggcaatgtg
     5941 tccgaacggg aagcccgtca gcacggggat tttcgccgtg cgcgaaacat ggttggcaac
     6001 ggcggaaaaa tcataagacg gatcatagac atctcgaatt ttttccatac ggaaattgcc
     6061 gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat tgagcatacg
     6121 ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc cgtcgatgtc
     6181 gggcatataa ggcgtaccgg cgagcgaggc gaggacgctt aagttgccgc cccacaacgt
     6241 gccttcggtt tcgacatcgg cgcgttggat ataaggaaca tcaacggtca ggcggttttg
     6301 ggttgcaccc ttgataaagg catccatcgt aaacgcaccg ggggcgggtt tgccaaaatc
     6361 gctataagcc atcgggccgg caaaactcat catattgcct tttgccaaca atgccagctg
     6421 gacggcgcat acgtcgctga atccgaaaaa cagcgtgccg tgttcgcgca tccttgcgcc
     6481 gagcgaagca aaatcgatat gcggcagaat gcgtgccgca ccataaccgc cgcgcaagcc
     6541 catcagcact ttaggtgtgg caacgcgacc ggaagcgaca tcttggaaat cggcggcacg
     6601 ttgtgagtcc gtgccggcaa accgttggaa acggcggctg cccgcctgtt ggttggttac
     6661 ggtaaaaccg acattgtaaa ggcgggttaa ggctgtgttg acgcggttgg tgtcttcggc
     6721 aaatcctgac gaagcgacaa cgcgcagaag gttgccgtcc gaactttgac ggcgtggcgt
     6781 ttggagaggc acggttcggg ctttcacaac ggaatgggaa gagggaaggg gcggaacgga
     6841 tgtggcggat gtgccgcaag cctgaagcag ccccgcgccg gcagcggcgg tgcaggtttt
     6901 cagaaaacgg cggcgggagg tcggttcggt cataatttcc ctttcatacg gtttcagacg
     6961 gcatcggcgg gcttaaagca gctttttaag ctgttccgtc caatcttgcg gcagggtcat
     7021 accgtgtttc cggacggggt ttgcccaaat cggcgcgggg aaggacgcat cgttttcaaa
     7081 gcgggcgata atatgccagt gcaggtgcgg cacgacattg cccaagctgg cgaggttaat
     7141 cttggcaggt tgaaacactt ggcgcatagc ggcttcgact ttgtacacca tttccatcaa
     7201 ttcgccgcgt tccgctgccg aaaggtcggt catttcggcg atgtgcttac gccaaatgac
     7261 gcggcagaat gcaggcgaac cgctgtcgtt atggatggcg atgacgcgga ggttgggggt
     7321 ttgcagcaaa atatcttcat cttgtgccgt gcagatgggg cagggttggg cggtcatttt
     7381 tccttattcc tgaaagtgtt taaaaaacgg taggataatt tctgatatag tggattaaca
     7441 aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggaac cgattcactt
     7501 ggtgcttgag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg
     7561 gtttaaattt aatccactat agtttaattt gtctgtccgc attgccgcta attgtaccac
     7621 ttcatcgtat ttccttttcg gttgaaaccc cgccactcgg acatctgtcc ttcggggcgg
     7681 caggatcaga ctttatttgg gagggggtgt aaccccttcc aaatcaggac gacacatagg
     7741 gaggtgcttt atgtgtcgtc ctgtgtgttg aaatatatcg gacgcgtttg aacagttgtg
     7801 cggcaaactg ttttagcggc aggcaagaga tgccgtctga tgccgtctga agcccattgt
     7861 ccgtcattcc cacacaggca gttgctacgg ttgctgaagc cccttagccg tcgcgcaggc
     7921 ggtt
//
LOCUS       DAXIHS010000084         7906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXIHS010000084 DAXIHS010000000
VERSION     DAXIHS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.084"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            52..591
                     /locus_tag="ACFOF2_001712"
     CDS             52..591
                     /locus_tag="ACFOF2_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC7107984.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(781..996)
                     /locus_tag="ACFOF2_001713"
     CDS             complement(781..996)
                     /locus_tag="ACFOF2_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7107985.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(1120..1479)
                     /locus_tag="ACFOF2_001714"
     CDS             complement(1120..1479)
                     /locus_tag="ACFOF2_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC7107986.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            1532..1908
                     /locus_tag="ACFOF2_001715"
                     /pseudo
     CDS             1532..1908
                     /locus_tag="ACFOF2_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            2107..2727
                     /locus_tag="ACFOF2_001716"
     CDS             2107..2727
                     /locus_tag="ACFOF2_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC7107987.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGILYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            2750..4201
                     /locus_tag="ACFOF2_001717"
     CDS             2750..4201
                     /locus_tag="ACFOF2_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC7107988.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            4663..5019
                     /locus_tag="ACFOF2_001718"
     CDS             4663..5019
                     /locus_tag="ACFOF2_001718"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC7107989.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGVYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            5010..5492
                     /locus_tag="ACFOF2_001719"
     CDS             5010..5492
                     /locus_tag="ACFOF2_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC7107990.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            5505..6098
                     /locus_tag="ACFOF2_001720"
     CDS             5505..6098
                     /locus_tag="ACFOF2_001720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC7107991.1"
                     /translation="MASIQNLYETVIGVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEDLHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            6088..7344
                     /gene="nuoD"
                     /locus_tag="ACFOF2_001721"
     CDS             6088..7344
                     /gene="nuoD"
                     /locus_tag="ACFOF2_001721"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC7107992.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            7344..7817
                     /gene="nuoE"
                     /locus_tag="ACFOF2_001722"
     CDS             7344..7817
                     /gene="nuoE"
                     /locus_tag="ACFOF2_001722"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC7107993.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
BASE COUNT         2004 a         1861 c         1849 g         2192 t
ORIGIN      
        1 tatagatacc taacccttgt ttatagctga aaatcaataa ggaagaacat tatgtcccaa
       61 gtttttaaag attttgattt gtcctccgta tgggaatccg acagttgggc agatgaaaac
      121 taccaagaag ccccgtttac ccctgaaatt ttggctgccg tagaaagtga actgggctat
      181 aaattgccgc aaagttttat tgaattgatg gcggtacaaa acggcggaat atttgtcaaa
      241 aactgttttc cgaccacgca gagaaattcg tgggcggaaa atcatgtgca aatttgcgag
      301 gtatcgggca tcggctttga aaaagaaggg agtttgtgcg gcgaaacagg gcaaaaactt
      361 tggctggaag aatgggaata tccgcccatc ggcgtgtatt ttgccaacga cccgtcaggc
      421 ggtcatgcca tgtttgcctt ggactatcgg gcgtgcggca aagacggcga gccgaaagtg
      481 gtgtttgtcg agcaagaatc ggattttgaa atcgtcgaac ttgcccccga ttttgaaacc
      541 tttatccgca gcttgcggca tgaagatgag tttattgacg aggaaatata aaacggcggt
      601 tgaaaaaccg gaatcatcaa gggaaaacgg gcgaaataac ggataatcgc ttgaatccgt
      661 aaggaaaacg gtttggtgga acgcgccatc caagaccttt gcaaagaaaa actgtctccg
      721 acagcattga cattattaac agaacttatc aagtttggag ctatctcaaa tataattcgg
      781 ttatcctttt gtatccatta aatcatatgc ttcaattaat tgttgttcta gctcttgtac
      841 caattttgaa ttgcgaattc ctgacacaat ctcaaattct tctgcatcta tgcaaacacc
      901 tgcataaatt tcaataacaa gggaacgcaa taattgaagc tcttctcttg ttaaagaaat
      961 aataatgtca tcacctgtgt aattgattat attcataata attttatttt tgtttgtcaa
     1021 agtaagtttt gcctaaggtt ggtctaaatg cagttccacc atcttttgaa tttgggtctc
     1081 tgattacaat tgctccagac ttatcatccc aaattgctct tatgtgtttg gattgtaatc
     1141 ttcgaattcc caagaaaaaa atcgtaataa gtttgaaagt gtcaaatccc aagtttcttt
     1201 tgagcaatat tctaatattt tatcaatttc acttttaata atcttatgat caaactgttc
     1261 taatattaat gcattagacc aaaaaaaacc ttctttatta caatgatggg aaatccattt
     1321 aggagaacaa atgcaaagtg aaaaaataga tgagccttgt tctccttcga ttccgatatc
     1381 caaatctatc catctatgga aattatctgg aatttcgggg gtaaattttt caaaatcaat
     1441 atcatataaa tttatgcttt ttaaatccaa tttaatcatt agggctgtcc tagataaata
     1501 gggaaattca aattaagtta gaattatccc tatgagaaaa agtcgtctaa gccggtataa
     1561 acaaaataaa ctcattgagc tatttgtcgc aggtgtaact gcaagaacag caacagagcc
     1621 tgacagcatt gtttatacgg attgttatcg tagctatgat gtattagatg tgagcgaatt
     1681 tagccatttc cgtatcaatc acagcacaca ttttgctgaa cgacaaaacc atatcaatgg
     1741 aattgagaat ttttggaacc gggcaaaacg tcatttacgc aagtttaacg gcattcccaa
     1801 agcgcatttt gagctgtatt taaaggagtg cgaatggcgt tttaacaaca gtgagataaa
     1861 agttcttgtt ccattttaaa acaattagta aaatcgagtt tatcttagtt gtccaggaca
     1921 gccccattat ttttataaca ccgtgaagcc gcacagcagt ttgaacagtg atacgccgtt
     1981 tgcgggctta cgagtttatt ttcccggcct gcagtttgag caatacggtg atttcctacg
     2041 gttaatacaa atgtttacac attgatacat ttcatttata gttccgccta tttgaaaata
     2101 gaaaatatga attcgaccgc aagtaaaacc ctgaaaggat tgtcgctggt gtttttcgcc
     2161 tctggattct gcgccctgat ttaccaggtc agctggcaga ggcttctatt cagtcacata
     2221 ggtatcgatt taagttcgat tactgtcatt atttctgtat ttatggtcgg cttgggtgtg
     2281 ggtgcgtatt tcggtggacg cattgctgac cgttttcctt caagtatcat ccccctgttt
     2341 tgcatcgctg aagtatccat cggtctgttc ggtttggtaa gcaggggtct gatttccggc
     2401 ttggggcatc ttttagttga ggctgatttg cccatcatcg ctgctgccaa tttcctctta
     2461 ttgctgcttc ctacctttat gatgggcgcg accttgccct tgctgacctg tttttttaac
     2521 cggaaaatac ataatgttgg cgagtctatc ggtatcttat attttttcaa cactttgggt
     2581 gcggcactcg gatcgcttgc cgccgccgaa tttttctacg tcttttttac cctctcccaa
     2641 accattgcgc tgacagcctg ctttaacctt ctgattgctg cttcagtatg gctgcgttac
     2701 agaaaggatg gatatagtga acactaaacc gaatactagt ttgatttata tgctttcttt
     2761 ccttagcggc ttattgagct tgggtataga agtcttgtgg gtaaggatgt tttcgttcgc
     2821 agcacagtcc gtgcctcagg cattttcatt tactcttgcc tgttttctga ccggtatcgc
     2881 cgtcggcgcg tattttggca aacggatttg ccgcagccgc tttgttgata ttccctttat
     2941 cgggcagtgc ttcttgtggg cgggtattgc cgactttttg attttgggtg ctgcgtggtt
     3001 gttgacgggt ttttccggct tcgtccacca cgccggtatc ttcattaccc tgtctgccgt
     3061 cgtcagaggg ttgattttcc cgctcgtaca ccatgtgggt acggatggca acaaatccgg
     3121 acgacaggtt tccaatgttt atttcgccaa cgttgccggc agtgcattgg gtccggtcct
     3181 tatcggcttt gtgatacttg atttcttgtc cacccaacag atttacctgc tcatctgttt
     3241 gatttctgct gctgtccctt tgttttgtac actgttccaa aaaagtctcc gactgaatgc
     3301 agtgtcggta gcagtttccc taatgttcgg catcctcatg ttcctactgc cggattctgt
     3361 ctttcaaaat attgctgacc gtccggatag gctgattgaa aacaaacacg gcattgttgc
     3421 ggtttaccat agagatggtg ataaggttgt ttatggggcg aatgtatacg acggcgcata
     3481 caataccgat gtattcaata gtgtcaacgg catcgaacgt gcctatctgc taccctccct
     3541 gaagtctggc atacgccgca ttttcgtcgt tggattgagt acaggttcgt gggcgcgcgt
     3601 cttgtctgcc attccggaaa tgcagtcgat gatcgttgcg gaaatcaatc cggcataccg
     3661 tagccttatc gcagacgagc cgcaaatcgc cccgcttttg caggacaaac gtgttgaaat
     3721 tgtattggat gacggtagga aatggctgcg tcgccatcct gatgaaaaat tcgacctgat
     3781 tttgatgaat acgacttggt actggcgtgc ctattccacc aacctgttga gtgcggaatt
     3841 tttaaaacag gtgcaaagcc accttacccc ggatggtatt gtaatgttta ataccacgca
     3901 cagcccgcat gcttttgcta ccgccgtaca cagtattccc tatgcatacc gctatgggca
     3961 tatggtagtc ggctcggcaa ccccggtagt tttccctaat aaagaactgc tcaagcaacg
     4021 tctctcccgg ttgatttggc cggaaagcgg caggcacgta tttgacagca gcaccgtgga
     4081 tgctgcagca caaaaggttg tctctcgtat gctgattcag atgacggaac cttcggctgg
     4141 ggcggaagtc attaccgacg ataatatgat tgtagaatac aaatacggca gagggattta
     4201 accgtcttaa agggtttcag gcaacgcagg ttttaggtaa cgtcctgcta gttcaaaaaa
     4261 accgcatcac agcagtcggg acaaaatggt ttaaacattt tgtcccgaat tcttattcct
     4321 atatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag
     4381 tacggaaccg attcacttgg tgcttgagca ccttagagaa tcgttctctt tgagctaagg
     4441 cgaggcaacg ccgtactggt ttttgttaat ccactatacc acgaattacg gtgtaaaaat
     4501 ttatatgacc ttataaaatc aaataagaat cgttatcata acatgattgt atttattggg
     4561 tttttttggg cgttttgccg atatttacct tttaatggtt tttgaaattc gctaaaatac
     4621 gaaattattg tagaaatttt gttaacggat ttgggtgtaa ccatgttgtc cgcttacttt
     4681 cccgtctttg tctttatcct tgtcggtctt gcagccggcg tactgtttat cctgctcggc
     4741 acaattttgg gcccgaaacg tcactatgcc gaaaaagacg cgccttacga atgcggtttt
     4801 gaagcctttg aaaacgcaag gatgaagttc gacgtgcgct attacctcgt cgccatcctc
     4861 ttcatcctgt ttgatttgga ggtcgcgttt atgctgccgt gggctgtcgt gttcaaagat
     4921 ttgggcgtgt acggcttctg gtctatgctg gtgtttatcg tcgttctgac ggtaggcttt
     4981 gtttacgaat ggaaaaaagg tgcgctggaa tgggaataga aggcgttttg aaaaaaggtt
     5041 tcatcaccac cagcgcggat acggtgctga actatatgcg taccggttca ttgtggccgg
     5101 ttactttcgg cttggcctgc tgcgccgtgg aaatgatgca tgcaggtatg gcgcgttatg
     5161 accttgaccg tttcggcatc attttccgtc cgtccccacg tcaggccgac ctgatgattg
     5221 tggcaggtac gctgaccaac aaaatggcgc ccgccctgcg ccgcgtatac gaccagctcg
     5281 ccgagccgcg ttgggtattg tctatgggct cgtgtgccaa cggcggcggt tattatcact
     5341 attcttattc cgttgtgcgc ggtgccgacc gcgtcgtgcc ggtagacgtt tatgtgccgg
     5401 gttgtccgcc gactgcggaa gccctgattt acggcctgat tcagctccaa caaaaaatca
     5461 agcgcacttc caccatcgcg cgtgacgagt aaggagagga cgatatggca agcattcaaa
     5521 atttatacga gaccgtcatc ggtgtgctgg gcaatcaggc aggcaaagtc atttcagctt
     5581 tgggcgagat taccgtcgag tgtctgcccg aacactatat ctcagtcatg accgcattgc
     5641 gcgaccatga agatttgcat ttcgagcttc tggtcgattt gtgcggtgtc gattacagca
     5701 cttacaaaaa cgaagtatgg cagggcaaac gctttgccgt cgtcagccag ctgctttccg
     5761 ttaaaaacaa tcaacgcatc cgcgtacgcg tctgggtttc agacgacgac ttccccgtag
     5821 tcgaatccgt agtcgatatt tacaacagcg cggattggta cgaacgcgaa gccttcgata
     5881 tgtacggcat catgttcaac aaccacccgg acttgcgccg catcctgacc gattacggct
     5941 ttgtcggaca tccgttccgc aaagacttcc cgatttccgg ctatgtggaa atgcgttacg
     6001 acgaagagca aaaacgcgtg atttaccaac ctgttaccat tgagccgcgc gagatcacgc
     6061 cgcgtatcgt ccgtgaggag aactacggtg gccaataaat taagaaacta caccatcaac
     6121 ttcggcccgc aacaccctgc ggcgcacggc gtattgcgta tgattttgga gttggagggc
     6181 gaaaccatcg tccgtgccga tccgcatatc ggcctcctgc accgcggtac tgaaaaactg
     6241 gctgaaacca aaacctatct gcaagccctg ccctatatgg accgcttgga ctacgtttcc
     6301 atgatggtca atgagcaggc gtattgtttg gcagtagaaa aacttgccgg tatcgatgta
     6361 cctatccgcg cccagtatat ccgtgtgatg tttgccgaag taacgcgcat cctcaatcac
     6421 ttgatgggca tcggttcgca tgccttcgac atcggcgcga tgaccgccat cctctacgcc
     6481 ttccgcgacc gcgaagagct gatggacttg tacgaagccg tttccggcgc gcgtatgcac
     6541 gcagcctact tccgtcccgg cggcgtttac cgcgacctgc ccgacttcat gcctaaatac
     6601 gagagcagca aattccgcaa tgccaaagta ttgaagcagc tcaacgaatc ccgcgaaggc
     6661 accatgctcg actttatcga tgccttctgc gaacgcttcc ccaaaaatat cgacacactc
     6721 gaaaccctcc tgaccgacaa ccgcatctgg aaacagcgta ccgtcggcat cggcgtcgtc
     6781 tcccccgaac gcgccatgca aaaaggcttt accggcgtga tgttgcgcgg ttcgggcgtg
     6841 gaatgggacg tgcgtaagac acagccttac gaagtgtacg acaaaatgga tttcgacatc
     6901 cccgtcggcg tcaacggcga ctgttacgac cgttacctct gccgtatgga agaaatgcgt
     6961 caatccgtac gcatcatcaa acaatgttcc gagtggttgc gtgtcaatcc gggtccggtc
     7021 attaccacaa accacaaatt tgctccgccc aaacgtaccg aaatgaaaac aggtatggaa
     7081 gacctgattc accatttcaa actctttacc gagggtatgc acgttcccga gggtgagacc
     7141 tacaccgctg tcgaacatcc gaaaggcgag ttcggcgttt acatcatttc agacggcgca
     7201 aacaaaccct accgcctgaa aatccgcgca cccggcttcg cccatctgca aggcatggac
     7261 gaaatggcaa aaggccacat gctcgccgac gtcgttgcca tcatcggtac gcaggacatc
     7321 gtattcgggg aggttgaccg ataatgttat ccgcagagtc cttaaaacaa atcgacacag
     7381 aactggcgaa atatcccgcc gaccaacgcc gttccgccat catgggtgcg ttgcgtatcg
     7441 cccaaaccga aaaaggctgg ctcgcccccg aaaccatcgc ttttgttgcc gactatatcg
     7501 gcatcacgcc tgcacaagcc tacgaagtcg ccactttcta caatatgtac gaccttgagc
     7561 ctgtcggcaa atacaaactg accgtttgta ccaacctgcc ctgcgccctg cgcggcggta
     7621 tggctaccgg cgaatacctc aaacaaaaac tcggtatcgg ctacggcgaa actacccctg
     7681 acggcaagtt tacccttgtc gaaggcgaat gcatgggcgc atgcggcgac gctcccgtta
     7741 tgctggtcaa caaccacagc atgtgcagct ttatgaccga agaagcgatt gagaagaaac
     7801 tggcggagtt ggagtaggtc gtctgaaacg acgatttaaa cgtaggtcgg atacttgtag
     7861 ccgacagagt gggtaaaaag gcaaaatgtc ggatttaaga atccgc
//
LOCUS       DAXIHS010000085         7873 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXIHS010000085 DAXIHS010000000
VERSION     DAXIHS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.085"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(232..648)
                     /gene="dksA"
                     /locus_tag="ACFOF2_001723"
     CDS             complement(232..648)
                     /gene="dksA"
                     /locus_tag="ACFOF2_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC7107994.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(795..1604)
                     /gene="proC"
                     /locus_tag="ACFOF2_001724"
     CDS             complement(795..1604)
                     /gene="proC"
                     /locus_tag="ACFOF2_001724"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC7107995.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1635..2042)
                     /locus_tag="ACFOF2_001725"
     CDS             complement(1635..2042)
                     /locus_tag="ACFOF2_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC7107996.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(2042..2728)
                     /locus_tag="ACFOF2_001726"
     CDS             complement(2042..2728)
                     /locus_tag="ACFOF2_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC7107997.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2848..3891
                     /gene="pilT"
                     /locus_tag="ACFOF2_001727"
     CDS             2848..3891
                     /gene="pilT"
                     /locus_tag="ACFOF2_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC7107998.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            4054..5280
                     /locus_tag="ACFOF2_001728"
     CDS             4054..5280
                     /locus_tag="ACFOF2_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC7107999.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRENMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHKQILIDIALNLSGIVCQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNINKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5566..7716
                     /gene="yccS"
                     /locus_tag="ACFOF2_001729"
     CDS             5566..7716
                     /gene="yccS"
                     /locus_tag="ACFOF2_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC7108000.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETADDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1923 a         2597 c         1726 g         1627 t
ORIGIN      
        1 cttctgcagc agcgaagaac cgaactatac gcccacaggg aaaaacggtc aatgctttca
       61 gtgggatttt tttggggaaa ttcgtcatgt cgctgtcgga taaggttttt tatttctgct
      121 aaatgctgcg ccgcctccaa caatcccttc ctctccctcc tctgactggt gcgcctttgt
      181 gaatatactg tctgaaactt agggcttcag acggcatttt gtttgccgcc atcagtcggc
      241 aaactgtttt ttcatcctct ctcggcgttc ttgggactca acagataaag tggctgtcgg
      301 gcgtgccagc agccgcttca gaccgatagg ctctcccgta tcggcacaga atccataatc
      361 cccttcatca atattgcgga tggtcgcctg tattttactg agaagttttc gttcccgatc
      421 gcgggtacgg agttccaatg cgtactcttc ttcctgtgtg gcacggtcgg caggatcggg
      481 ggctgattcg tgttcttgga gatgccctgt cgtagcggaa gcattttcga tgagttcgtc
      541 ttgcattttt accagcaatt cgcggaaaaa agccaaatgg tcgtcattca tataatcgtc
      601 ttccggcccg ctccaattca aaatatcttg ttctgtcagc tttgccataa tgtatatcct
      661 ctcggttcac cgaaaaaaac aaagcaccac aaagtggctt tattcctttg atttttataa
      721 ggcttattgc ctaaggcgtt tttgaaaacg cgcatcataa cacgttttgg attggttttt
      781 ttattttctt tacattattg atattgccgt tccatttcct gcgaacggcg cacacaggca
      841 caaacgccct cacttatggc ttcggcgaca cgatgccgcc tgaaggcttc cacggcttcg
      901 tgggttgtcc cgccttttga cgttacattt ttttgaagct tctcgaaatc ttcacccgtc
      961 tgctcggcaa gggtaaccgc tcctttaaac gttgccagac tgagcgcgcg tgcttctgcc
     1021 atatcaaacc cttgtcggat ggcggcattt tgcaatgcgt ccagcagata aaacacatat
     1081 gccggcccgc tgccgctgat accggtaatg ctgtgcattt gcgcttcatc ttccaaccaa
     1141 acagtcaaac cgactgattt catgattcga tcggcaatcc tgcggtctgt ttccgatact
     1201 tccgcttcgg catacatacc ggatacgccc agcccgattt ttccgggtgt attcggcata
     1261 acccggacaa tgcggcgtgt tcccccgagg taacggctga gcgtatcgac cggcaatccg
     1321 gctgcgacag aaagcaccaa tgcgccgttg gtgcggatat ttttgcacgc ggcttccata
     1381 tcctgcggct tgacggcaag gattaaaaca tcgtcggaat gaagctccgg caaggtttca
     1441 gaagtttcga cccccaactc tttttgcaaa cgttcgcgct tttccgcacc ccggttggca
     1501 atatggatgc ggtaaccgcc ttgtttgacc aatccgcccg caacggcagc cgccatattg
     1561 ccgccgccga gaaaataaac attcattatt tctaccctat tcaaaattac ctgactaaat
     1621 tcattcccaa ttttttacca tcggacgcgc tcggacagac cgttgcgccg caacccctgc
     1681 ttttccaagc agtcaaatcc tccctgtttg aacgtctcaa tcaaaggttt gccgtcacgc
     1741 gtacgccaat ccagcccgca ggcttcaaac ttccctattt tcctgtaaaa gtaagattgt
     1801 tgtgcggatt catattcgtt tgccctgacg gaacgattgc cgtctgaaag cggtatctcg
     1861 tagtcctcta aagacggcgg actctcgccg ccaatgtccc aaaaatcgat ggcagccggt
     1921 tttctcggct tacaccagcc ggacagggac gaacactcat accaacccgc caactggcag
     1981 cctgcaaccg taaatgcaag caacgcagcc attgccgtat attttattgc ccgaatttcc
     2041 atcagcccct tttcccgaaa atcgcgctgc cgatacggac gtgtgtcgca ccgcactcaa
     2101 tggcggcagg catatcgtcc gacatcccca tagacagcac gtctgcctta acgccagccg
     2161 tattgaggtc ggcaagcagt ttccgcatcg tttgaaattg caccttcaac tccgtttcac
     2221 tgctgttggc tttggcaaca cacatcagtc cgcgtacgac gatattcggc agcttcgcca
     2281 cttccacagc aagcgcgact gcttcttcgg gcgcgacacc gtgcttcacc gcctcgcccg
     2341 caatattcac ctcgatacac acctgcaagg gcggcattga ggaaggacgc tgttcgctca
     2401 gccggacagc ggttttcaga cggcatacgg tatgcaccca atgcgcgcgt tcggcgacaa
     2461 acttggtttt gttggactgc acatcgccga tgacgtgcca cacgatgtcg gtcaaatccg
     2521 ccaactcttc cgttttgccg taccactcct gaatatagtt ctcgccgaaa tcacgctgtc
     2581 cggcggcgta aacttcgcgg atgccgtctg aagggaaagt cttaccgacg gcaatcaggc
     2641 tgacggaatg cggcttcctt cccgcctgca gaaccaattt tccaatacgg tcggacacct
     2701 cccgataacg ttcttgcaac accgtcatag attatcccct aattaaaaat gattaaacag
     2761 ttgaaacccc tccagtcagg gcggtacaat caaggttgtt agaaccattc caaccaatcg
     2821 aaacattata ctaaacagag ccgcattatg cagattaccg acttactcgc cttcggcgct
     2881 aaaaacaaag catccgacct tcacctgagt tcgggcatat cccctatgat tcgggttcac
     2941 ggcgatatgc ggcgcatcaa ccttcccgaa atgagcgcgg aagaggtcgg taatatggta
     3001 acttcggtga tgaacgacca ccagcggaaa atctaccagc aaaacttgga agttgacttc
     3061 tcgttcgaac tgcccaacgt cgcccgattc cgcgtcaacg ccttcaacat cggccgcggt
     3121 cccgccgccg tattccgcac cattcccagc accgtcttat cgctggaaga attgaaagcc
     3181 ccgagcattt tccaaaaaat cgcagaatcg ccgcgcggca tggtattggt taccggccct
     3241 accggttcgg gcaaatcgac cacgcttgcc gcaatgatca actacatcaa cgaaacccag
     3301 ccggcacaca tcctgaccat cgaagacccg atcgaattcg tccatcaaag caaaaaatcc
     3361 ctgattaacc agcgcgagct gcaccagcac accctcagct tcgccaacgc gctgcgttcc
     3421 gcattgcgcg aagaccccga cgttatcctt gtcggcgaga tgcgcgaccc agaaaccatt
     3481 ggcttggcac tgaccgccgc cgaaaccgga cacttggttt tcggcacgct gcacacgacc
     3541 ggcgcagcaa aaaccgtcga ccgtattgtg gacgtattcc cggcgggaga aaaagaaatg
     3601 gtgcgctcta tgctgtccga atcgctgacc gccgtcatct cccaaaacct gctgaaaacg
     3661 cacgacggca acggccgtgt cgcctcgcac gaaatcctga ttgccaaccc cgccgtccgc
     3721 aacctcatcc gcgaaaacaa aatcacgcag attaactccg tcctgcaaac cgggcaggcg
     3781 agcggtatgc agacaatgga ccaatcgctg caatcgctgg tgcgccaagg gctgatcgca
     3841 ccggaagtcg cacgcagacg cgcgcaaaac agcgaaagta tgagtttctg acacacaacc
     3901 gccttccggc cataccggcg ggaaaacaag gcgcaaacac gcggggcggg acgcagcatc
     3961 ccgcccggct acctttccga acaagaagcg tccgccttcc tgttgaaacc tgccgccgca
     4021 aactgcaagg cttaaaccga aaagaagcta acgatgaata ccgataacct gcacgacatc
     4081 ttggacgaaa tggttcaagt gtattctcaa aaaaaacaaa gccgatccga aaccccggcc
     4141 gaaatcggcg cacacttcca cccgctgctc gaccgcctgt gcgaaaccgc agaagcacaa
     4201 aacgcgtccg acatccttat cagcaaagga ttcccgccct cgttgaaaat caacagcgca
     4261 ttaaccccgc agccgcaaaa ggcgctgacg ggcgaggaaa ccgccgccat cgccgcatcg
     4321 acgatgaacg ccgaacaatc ggaaatattc cggcgcgacg gcgaaatcaa ctactccgtc
     4381 cagtcgcgca gcggcacgcg ctaccgcgcc aacgcctacc acagccaagg cagcgcaggt
     4441 ttggttttgc ggcgcatcaa ccacgtcatc ccgcaaatgc aggaattggg cctgcccgaa
     4501 aaactcaaag acctcgccgt cgcaccgcgc gggctgctga ttatcgtcgg gcctaccggt
     4561 tcgggcaaat ccaccacgat ggcgactatg ctcgaacacc gcaacaaaac cctgcccagc
     4621 catatcgtta ccatcgaaga cccgattgag tttatctaca aaccgcgccg ctgcatcttt
     4681 acccagcgcg aaatcggcgt cgacaccata aactggcaga cggcggtaca aaacgctatg
     4741 cgccagtccc ccgacgtggt ctgcatcggc gaagtccgca gcagggaaaa tatggaatac
     4801 gcgatgcagc tcgcccaaac cggccacctg tgcattttta cgctccacgc caacaccgcg
     4861 ccgcagtcgc tcgaacgcat actcaacttc taccccaaag aacagcacaa gcaaatactg
     4921 atcgacatcg ccctcaacct gtccggtatc gtctgccaac gtctcgccct caaaaaagac
     4981 aaaaccggca ggacggcggt tgtcgatttg ctcatcaaca cgcccgccat ccaagacttc
     5041 atcctgaagg gcgacctgat gaacatcaat aaaatcatgg aaaccgccaa aaccgacgga
     5101 atgcagacga tggaccaaaa ccttttcgaa ctgtaccgtc acggcatcat cagttacgaa
     5161 gaagccctgc gccagtccgt ttccgccaac aacctgcgat tgcacatcca actgcacaaa
     5221 gaaggcaaaa cgcccgaact cctttacgac agggtcaacg gtctcaacct catttcctga
     5281 tccgcaaaac ccaatgccgt ctgaaaaccg catccccgtt ttcagacggc atgattttat
     5341 ccgccccatt catgtgctac actttatagt ggattaaatt tatagtggat taacaaaaat
     5401 caggacaagg cgacgaagcc gcagacagta caaatagtac ggaaccgatt cacttggtgc
     5461 ttcagcacct tagagaatcg ttctctttca gctaaggcga ggcaacgccg tactggtttt
     5521 tgttaatcca ctatattcac ttcataacaa taaaccggta aaaccatgaa aaccccactc
     5581 ctcaagcctc tgctcattac ctcgcttccc gttttcgcca gtgtttttac cgccgcctcc
     5641 atcgtctggc agctaggcga acccaagctc gccatgccct tcgtactcgg catcatcgcc
     5701 ggcggccttg tcgatttgga caaccgcctg accggacggc tgaaaaacat catcaccacc
     5761 gtcgccctgt tcaccctctc ctcgctcacg gcacaaagta ccctcggcac agggctgcct
     5821 ttcatcctcg ccatgaccct gatgaccttc ggctttacca ttttaggcgc ggtcgggctg
     5881 aaataccgca ccttcgcctt cggcgcactc gccgtcgcca cctacaccac acttacctac
     5941 acccccgaaa cctactggct gaccaacccc ttcatgattt tatgcggcac cgtactgtac
     6001 agcaccgcca tcatcctgtt ccaaatcatc ctgccccacc gccccgtcca agaaagcgtc
     6061 gccaacgcct acgacgcact cggtggctac ctcgaagcca aagccgactt cttcgacccc
     6121 gatgaggcag cctggatagg caaccgccac atcgacctcg ccatgagcaa caccggcgtc
     6181 atcaccgcct tcaaccaatg ccgttccgcc ctgttttacc gccttcgcgg caaacaccgc
     6241 cacccgcgca ccgccaaaat gctgcgctac tacttcgccg cccaagacat acacgaacgc
     6301 atcagctccg cccacgtcga ctaccaagag atgtccgaaa aattcaaaaa caccgacatc
     6361 atcttccgca tccaccgcct gctcgaaatg cagggacaag cctgccgcaa caccgcccaa
     6421 gccctgcgcg caagcaaaga ctacgtttac agcaaacgcc tcggccgcgc catcgaaggc
     6481 tgccgccaat cgctgcgtct cctttccgac ggcaacgaca gccccgacat ccgccacctg
     6541 cgccgtcttc tcgacaacct cggcagcgtc gaccaacaat tccgccaact ccagcacaac
     6601 ggcctgcagg cagaaaacga ccgcatgggc gacacccgca tcgccgccct cgaaaccggc
     6661 agcctcaaaa acacctggca ggcaatccgt ccgcagctaa acctcgaatc aggcgtattc
     6721 cgccatgccg tccgcctgtc cctcgtcgtt gccgccgcct gcaccatcgt cgaagccctc
     6781 aacctcaacc tcggctactg gatactgctg accgcccttt tcgtctgcca gcctaactac
     6841 accgccacca aaagccgcgt ccgccagcgc attgccggta ccgtactcgg cgtaatcgtc
     6901 ggctcgctcg tcccctactt caccccgtct gtcgaaacca aactctggat tgtcatcgcc
     6961 agtaccaccc tctttttcat gacccgcacc tacaaataca gtttctccac cttcttcatt
     7021 accattcaag ccctgaccag cctctccctc gcaggtttgg acgtatacgc cgccatgccc
     7081 gtacgcatca tcgacaccat tatcggcgca tcccttgcct gggcggcggt cagctacctg
     7141 tggcccgact ggaaatacct cacgctcgaa cgcactgccg cccttgccgt atgcagcaac
     7201 ggcgcctatc tcgaaaaaat cgccgaacgc ctcaaaagcg gcgaaacggc cgacgacgtc
     7261 gaataccgcg ccacccgccg ccgcgcccac gaacacaccg ccgccctcag cagcaccctt
     7321 tccgacatga gcagcgaacc cgcaaaattc gccgacagcc tgcaacccgg ctttaccctg
     7381 ctcaaaaccg gctacgccct gaccggctac atctccgccc tcggcgcata ccgcagcgaa
     7441 atgcacgaag aatgcagccc cgactttacc gcacagttcc acctcgccgc cgaacacacc
     7501 gcccacatct tccaacacct gcccgaaacc gaacccgacg actttcagac agcactggat
     7561 acactgcgcg gcgaactcga caccctccgc acccacagca gcggaacaca aagccacatc
     7621 ctcctccaac agctccaact catcgctcgg cagctcgaac cctactaccg cgcctaccga
     7681 caaattccgc acaggcagcc ccaaaacgca gcctgaaaaa gtttcggcat tttgtaagag
     7741 agggcagatt gtcagacagg ttgcaagata gtggataaag ctttgcgcag ggtaaatgcg
     7801 tagcaactga accgtcattc ccacgaaaac agaaaaccaa aaacagaaac ctaaaatccc
     7861 gtcatttcca cga
//
LOCUS       DAXIHS010000086         7464 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXIHS010000086 DAXIHS010000000
VERSION     DAXIHS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.086"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(71..1483)
                     /gene="trkA"
                     /locus_tag="ACFOF2_001730"
     CDS             complement(71..1483)
                     /gene="trkA"
                     /locus_tag="ACFOF2_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC7108001.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1581..3104)
                     /locus_tag="ACFOF2_001731"
     CDS             complement(1581..3104)
                     /locus_tag="ACFOF2_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC7108002.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRIDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLDKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            complement(3411..4217)
                     /locus_tag="ACFOF2_001732"
     CDS             complement(3411..4217)
                     /locus_tag="ACFOF2_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC7108003.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4269..4520)
                     /locus_tag="ACFOF2_001733"
     CDS             complement(4269..4520)
                     /locus_tag="ACFOF2_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108004.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4620..5381
                     /locus_tag="ACFOF2_001734"
     CDS             4620..5381
                     /locus_tag="ACFOF2_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108005.1"
                     /translation="MDVNKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRDHVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMALFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5680..6849
                     /gene="metZ"
                     /locus_tag="ACFOF2_001735"
     CDS             5680..6849
                     /gene="metZ"
                     /locus_tag="ACFOF2_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC7108006.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            6944..7150
                     /locus_tag="ACFOF2_001736"
     CDS             6944..7150
                     /locus_tag="ACFOF2_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC7108007.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7249..7361)
                     /locus_tag="ACFOF2_001737"
                     /pseudo
     CDS             complement(7249..7361)
                     /locus_tag="ACFOF2_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1614 a         1837 c         2158 g         1855 t
ORIGIN      
        1 gtctgaagcc cacctaccat tatttctgct tccataccgc ttggcggcaa tgtgtccgga
       61 atggggcggt ttatccgaaa aagcccattt tgacttggat gagtttttcc agttcgttca
      121 ggatgcgccg gcgcgagacg aaaaagatga tgtggtcgcc gtcttggatg acggtttcgg
      181 tatggtgtcc cataatggtt tcgccggttc cggcgcggac gacggcggca atgtggcagc
      241 cttcgggcca tttgatgccg ctgatgcgcc tgccgatgat ggcggaagtt tttttgtcgc
      301 cgtgtgcgac gacttcgatg gcttccgccg tgccgcgccg gatggggtgg acggcaacga
      361 tgtcgccgcg ccggatgtgg gcgagtatcg agccgatggt gatgaggtgg ggggagacga
      421 cgatgtcgat tttgttgcct tcgagcaaat cgacgtagct tgagcggttg acgatgccga
      481 tgacgcgctt cgcgccgagg tttttcgcca aaagggcgga cataatgttg ctttcgtcgt
      541 cgttggtcag ggcgcagaat acgtcgattt cgtcgatgta ttcgttgtcg agcagggttt
      601 cgtcggttgc cgaaccttgc aggacgaggg tgttgtcgag gttttcggct atccattcgg
      661 cacggcgcgg ccgacattcg atgattttga cgttgtatgc gtgttcgagc tgcttggcga
      721 gacggtagcc gatgttgccg ccgccggcaa tcatgatgcg gcgggtgctg gtttctttgg
      781 ggcgcaattc gggtatgacc gcgccgatgt tttcggcggc ggcggcaaac aggatttcgt
      841 cgccttcgat gatgacggtt tgcggcgcgg ggacgatgag gcggttgttg cggtaaacgg
      901 cacagatttg gcagtcggcc ccgtcgggca aatcttgggc gatgtcggca atgctgcgtc
      961 cgacaagcag tccgccgcgc cgcgcctgta tgatgaccat ccgcacgcgg tcgtctgcaa
     1021 aacgtaaaac ctgcaatgcg cccgggcagt ctatcaggcc ggcaagctgt tcggtaacga
     1081 gctgttcggg gctgatggtt tcggttatgc cgaatatgga aaggctgccg ttttcgttgt
     1141 tttcgagctt ggggctgagg tattcgaggt attcgctgga acggacgcgc gcgatgcggc
     1201 cggggatgtt gaacaggtcg gcggcaactt tgcaggcgac gatgttggtt tcgtcgctgc
     1261 gggagagcgc gagcagcaag tcggcatctt ccgcgccggc gcgttctaat gtgaaggggg
     1321 atgcgccgtt gccgaaaacg gtttggacat cgaggcggct gcctgtttcc tgcaatgctt
     1381 tttcgtcgat gtcgataacg gttacgtcgt tgttgggtat ggcggcaagg ttttgtgcga
     1441 cggtagaacc tacctgtccg ttgcctaaaa tgaggatttt cacggtgtgg gtcgccgagt
     1501 gatgaaatgg tggattgtac cgcaaccggg ggcggaaatg ccgtctgaaa gcagattttg
     1561 tgttcagacg gcatggcgcc tcaagattcg acggggatga tgccgatttt cgcctgccat
     1621 tggggcgggg cggtggcgtg gatggattcg cctttgctgt ccacggcgac ggttacgggc
     1681 atgtctttga cttcaaattc gtaaatggct tccatgccca attcggggaa cgccaagact
     1741 ttggaagatt tgatggcttt tgccacgaga tacgccgcgc cgccgaccgc catgaggtac
     1801 acggctttgt tgtcggcaat ggcttcgcag gtggccgcgc cgcgctcgga tttgccgatc
     1861 atacccaaga ggccggtttg ttcgagcatt tggcgggtga atttgtccat acgggtggcg
     1921 gtggtggggc ctgccgggcc gacgacttca tcgccgaccg gatcgacagg gccgacgtag
     1981 taaatcaggc ggttggtgaa atcgacgggc aattcttcgc ctttgtcgag catatcgacg
     2041 aggcgtttgt gtgcggcatc gcggccggtg aggattttgc cgttcaacag caatacgtcg
     2101 ccggttttcc agctcgccac ttcttctttg gtcagcttat caacatcaat gcgtttgccg
     2161 ttgtcggggc tgtaagtcaa atcgggccag tcttcgacgc gcggtggagt cagctcgaca
     2221 gggcctgagc cgtccaattc aaactctacg tggcgggtgg cggcgcagtt gggaatcatc
     2281 gcaatcggct tagaagcggc atgggtcggg taatcgagga ttttcacgtc caacacggtg
     2341 gtcagaccgc ccaagccttg cgcgccgatg cccagcgcgt tgactttttc aaagagttcg
     2401 aggcgcaggg cttcggtggt ggacaattct gcacctgacg ctgctttttc ctgcaactct
     2461 tgaatgtcga tgtggctcat caaggattct tttgccatca acacggcttt ttcgggcgtg
     2521 ccgccgatgc cgatgcccaa gatgccggga ggacaccagc ccgcgcccat cgtcgggatg
     2581 gttttcaata cccaatcgac gatgttgtcg gaaggattga gcatggcgag tttggatttg
     2641 ttttcagaac cgccgccttt tgccgcgcag gttacttcga ctttgtcgcc cggcacgatg
     2701 ctcatatgga tgacggcggg cgtgttgtct ttggtgtttt gacgtttgcc ggccgggtcg
     2761 gcgagaacgg aagcgcgcag cgtattgcct tcccaagtgt aggcgcggcg tacgccttcg
     2821 ttaaccattt cttccacgct catgtccgca tcccactgca catccatacc gactttcaaa
     2881 aacacggttg cgatacctgt gtcttggcag atggggcggt tgttttcggc acacatgcgg
     2941 ctgttgacca aaatctgcgt catcgcgtct ttggcggcgg ggttttcttc tttctgccac
     3001 gctttataga gcgcgtcgat gtagtctttg ggatggtagt agctgatgaa ttggaaggca
     3061 tcgcaaatgc tttggataaa gtcttcctgt ttgatgacgg tcatgattgt gttccttttc
     3121 aaggttgtgg cggtaagtgt ctgctgttgt tttaagcaac ctttttgaag gctgtttttt
     3181 atcaggttga aatagtgcca gcgtttgtac ggtaaagaca atacataatt ctaatctctt
     3241 gattatcaat ttgttttaat aaaatactgt tttactcgga ttttgatatt tgtaatcttt
     3301 gcctgttgct ttgcggtagg gagaaatcag caggcagttc attgtaggcc gattgatgaa
     3361 caatgccgtc tgaaagggct tcagacggca ttgtgttcgg atgggcgggc ttattttgcg
     3421 gcctgtccgt cttcttttgc aaaatatttg gcgtagattt tgtcgtattc gccgctttcg
     3481 cgtacttttt tcaacgcatc gttcagcatt ttgacggttg cttcgtcgcc tttgcgtacc
     3541 gcgatgccgt agtgttcggt ggtgaagtcg ggcagggtaa cgaagtccat tcctttggtc
     3601 ggattgtttt tcacataatt ggcgatgacc gcgctgtcgc tgactacgga atctaagccg
     3661 ccgttttcca gttctttgat aatcagggga acgttttcaa agcgcgcgat tttcgggttg
     3721 tcgttgccca agagtttgga tacggagaaa tcgcccgtgt agccggttac cacgccgact
     3781 ttgttcatgt ttttcaaatc ttcggaagaa gatatttttt tgcctttcgg aacgaggacg
     3841 acttgggtga tttcaaaata cgggtcgctg aagtccatag actgtttgcg gtcgtcggta
     3901 atggttacgc ccgacatcac aacgtccgca tcgccgttgt tcaaggcggg gaaaaggctg
     3961 tcccacggct ggtgtttgaa ttcgatttta aaattgcccg ccttcgccat cgcgttcatc
     4021 aaatccacat cgaaaccttc gacattgcct ttcgagtcta aagattcaaa gggggcaaac
     4081 tcggcgttgg aagccacgcg gtacactttg tcggggtttg cggcgggcgc ggcggcatct
     4141 ttaccctgac cgccgcaggc agacagcgcg agcgcggaac aggcaagggc ggcggcaatc
     4201 cattttttca tattcatcaa aattcctttc ggtaatcaaa aagcctaaaa aatcagttta
     4261 acataaaatt aacgttcttt ttcacggaaa aacgcataag taacgtcgtg gaacaaatcg
     4321 gtatgggggc tgtcctcata gctcaattct gccaaggcgt cctcaaaagc aagctgaatc
     4381 gactcgaccg cgatttcggc ggtttcggcg ggaagggcgc ggattaaggc tttgaatgcg
     4441 gtggacagga cgtggttctg catacgcagc acgtcgttcg cctcttccag atattccaaa
     4501 cgctgttcga tgctgcccat gatgtatcct cgcgaatctg ttaaaatgcg ccattatatc
     4561 aaaccgcgct ttgggcgcgg gtttgtctgc ctgcaaagat taagaaccgg aagccgaaaa
     4621 tggatgtgaa taaagagccg aattgggaaa aacgtttgga aaacctgcgt cagacgcgcc
     4681 gtatgaaggc agtgccgact tacgcgccgc tgtcccgccc ctctgaaacc ggaacggacg
     4741 cgcctgtgcc gaaactggac gatgagagcc gagaccatgt gttgaaggcg tatctgaaaa
     4801 aatggcagga ggaacacaat gccgccgttg ccgattgcgg cgaggaagtc gaaaccgacc
     4861 cgatgattgt ccttcaggaa aactggctca acgcgcaggc ttccctgcaa atgtcggttt
     4921 cggaaaaaca catcgaatcg cggcggagaa tcctgttcga tgccaaaaaa gacagtgccg
     4981 aatttgaaac cggacagatt gaaggcgggg aaaacgccga tgcggcagaa tcgggtacgg
     5041 cggcagacgg ggttgcggaa aacggcgaag gcggtgcgga agaggcgttg atgcccgtcc
     5101 aaatcaatat cctgaacccg aaagccgtca accggcgcga agtgttctgc ctgtccgaac
     5161 aggagctgac cgaacggctg atcaaacgcc tgcgtccgca tctgaccgat accgtcaacg
     5221 gcatcattcg aacggcggtg caaaagcaga tggcactctt tacttatcag ctccagcaaa
     5281 tgctgcacga gcaggcgggc gcggtggtcg aagacgtgtt ggaacacgat gtccgcaaaa
     5341 tcctcaacga catcaagtac gagttaaaat acaaacgctg accgtcatgc cgtctgaaag
     5401 ccgtgttcag acggcatttg cattctcatc cgacttgaaa gcctcacgga acgggcgcaa
     5461 aatattaata tagtggatta acaaaaatca ggacaaggcg acgaagccga agacagtaca
     5521 aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
     5581 taaggcgagg caacgctgta ctggtttttg ttaatccact ataaattaaa caacgggcgc
     5641 acgctccgtt gccgaacgga taaaaccgag gaatcaccga tgagcaaaaa actccatccg
     5701 caaacgctcg ccatacgcgg cggcaaagaa caaaccggat accgcgaaca caatcaggca
     5761 ttgttcctga ccagcagttt catgtgggac aacgcccaac acgctgccga tttgttttca
     5821 aaaaaaatca aaggcttcac ttatacccgt accgccaacc cgaccacagc cgcctttgaa
     5881 aaacgcatcg ccgctttgga aggtgcggaa cgcgcggtcg ccacttcgac cggcatgtcc
     5941 gcgattcagg cggcgttttt cacctttttg caggcgggcg accatgtgat ttccagccgc
     6001 agcctgttcg gcacgaccgt cggctttatc aataacatcg ttaccaaatt cggcatcgaa
     6061 gtgagtcacg tgtcgccgac tgatataaac gaatggaaag ccgccgtcaa agccaacacc
     6121 aaactgctgt ttttggaaac gccgtccaat cccttgggag aagtggccga cttggaagcc
     6181 ttggcggaat tggcgcacgg catcggcgcg ctcttggtgg tggacaacag cctgctgtcg
     6241 cctgtcggct ctcagccttt gaaacacggc gcggatattt ccgtttcctc cgcgaccaaa
     6301 gccatagacg gacacgggcg cgtgatgggc ggcgtgttgg cgggttcgga agaactgatg
     6361 gcgcaggtcg ccgtttactg caactcttgc gggctggcaa tgtcgccgtt taacgcatgg
     6421 cagttgttga gcggcgtgga aaccctgtct ctgcgtatgg aaaaacagtt cgacaacgcc
     6481 ttgaaaatcg cgcaatggct gcaagcgcag ccgcaggttc aagccgtgta ttacaccgga
     6541 ttacccgacc atcctcaggc ggagcttatc cgcaaacagc aaaacggcgg cggcatcgtc
     6601 atcggctttg aagtcgccga ccaagcagcc gcgtggaaag tcgtggacgg cgtcgaactt
     6661 ttctcccgta ccgccaacct cggcgacgtg cgctcgacca ttacccatcc gtggacaacc
     6721 acgcacggca ggatgcagcc cgaagaaaaa ctcgccgcca acatccgccc cggattggtg
     6781 cgcctgtcgg tcggtttgga atatgttggc gatttgattg acgatttgaa acaggcactc
     6841 gcgcgctgat tgcgtgttca gacggcatcg gcggaaaaac accgcaaatg ccgtctgaaa
     6901 cccttatctt atatgacata cacgtcaaca ataggaaaaa aatatggatg aagcggtttt
     6961 tgccgattgg gcgttgaaaa tctgcctgac cggcttgatt atttttttgg gttttatcgt
     7021 ttggaatttg ggcaaggagt ctaaagcggg caaattcggc atcgccattt tgtttttggt
     7081 tttgggcttg ggcgtgtttg gttttgtgtt caaagagtta ttgattaagt ttttggtatt
     7141 gccgaaatag gtttgcggtc tgtcaaaatg ccgtctgaaa tcataaaaag taatctgcac
     7201 cctaaaagtt tgatttaacc gccaactgat taaggtgcgg atttttatga caaaatatag
     7261 tggattaaca aaaaccagta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc
     7321 ggcttcgtcg ccttgtcttg atttaaattt aatccactat aaaaatcatc tttccgcagt
     7381 cattcccacg gatgcgaaag cctgtctttt tgaatcccgg ataattttac atcgtcattc
     7441 ccgcgcaggt gggaatccag atca
//
LOCUS       DAXIHS010000087         7399 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXIHS010000087 DAXIHS010000000
VERSION     DAXIHS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.087"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(229..858)
                     /gene="nth"
                     /locus_tag="ACFOF2_001738"
     CDS             complement(229..858)
                     /gene="nth"
                     /locus_tag="ACFOF2_001738"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC7108008.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(904..1317)
                     /locus_tag="ACFOF2_001739"
     CDS             complement(904..1317)
                     /locus_tag="ACFOF2_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC7108009.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            1907..3130
                     /locus_tag="ACFOF2_001740"
     CDS             1907..3130
                     /locus_tag="ACFOF2_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC7108010.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            3421..4800
                     /gene="nhaC"
                     /locus_tag="ACFOF2_001741"
     CDS             3421..4800
                     /gene="nhaC"
                     /locus_tag="ACFOF2_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC7108011.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4929..5753)
                     /locus_tag="ACFOF2_001742"
     CDS             complement(4929..5753)
                     /locus_tag="ACFOF2_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC7108012.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(5755..6270)
                     /gene="ybeY"
                     /locus_tag="ACFOF2_001743"
     CDS             complement(5755..6270)
                     /gene="ybeY"
                     /locus_tag="ACFOF2_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC7108013.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAANLPSEHDFYRWAWSAL
                     KSGYRRADISLILLDEEAARAYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            6389..7324
                     /gene="hemC"
                     /locus_tag="ACFOF2_001744"
     CDS             6389..7324
                     /gene="hemC"
                     /locus_tag="ACFOF2_001744"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC7108014.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
BASE COUNT         1549 a         1800 c         2058 g         1992 t
ORIGIN      
        1 ccgcaccgaa aaagctgccg gctttgtcgc agcctgccag cgaggcggca cacagtgctg
       61 ccaaagcgag gtattggtat tttttgaaca cgttttgtcc tttgtcggat gccggtaccg
      121 gctttaatgc cgtctgaagc gcattttgtc ggcttcagac ggcataggtt gaaattctac
      181 aacgtccgtc cgaattttca agcgtttcat tttgaagggc ggcggcggtc aggctttggc
      241 gggatattcg cacaaatcgt tgatgatgca ggtttggcat tgcggtttga gtgccttgca
      301 ggtgtagcgt ccgtgcaaaa tcagccagtg gtgcgcgtcc atcagaaatt ctttaggaat
      361 gaagcgcatc agtttgtctt cgacttcgcg cacatctttc ccgggggcga ttttggttcg
      421 gttggacacg cggaaaatat gcgtatcgac cgccataacg gggtgtccga acgctgtgtt
      481 caataccacg tttgccgttt tgcgccccac gcccggcaac gattccaaag cctcgcggtc
      541 ttccggcact tcgccattgt atttttccag caggatgcgg caggtttgca tgatgtgctt
      601 ggacttggtt ttatacagcc cgatggtttt cgtgtattcc atcacgccgt ccaaacccaa
      661 atccagcatc gcctgcggcg tatcggcaac cggaaacagc ttcgccgtcg ctttgtttac
      721 gccgacgtcg gttgcctgtg ccgacagtaa aacggcgatt aaaagctcga aaggggagtt
      781 gaagttgagt tctgtggtcg gatgggggtt ggcggcgcgg aagcgttcga agatttcttg
      841 gcggatgtgt ctgttcattt ttttatacgg tgggtttgtg tgttcggcat tataacgtat
      901 ggttcaggcg gcgtaatatt gcattcccca cagaatgaag gcgtaacgcg ccgttttgcc
      961 gataaccagc atcagcccgc ttgtccacgg attcaaccgc agccagccgg cggcaagcgg
     1021 cagtgcgtcg ccgacgacgg gcagccaggc aaacgcaagc agccaaatac cgaaacgccg
     1081 catcagattc agtgtttttt cagacggcat ttttcgggag ggcagcaaac gccccatcca
     1141 ataggaaacc atactgccca atccgttggc aaggccggcg cacagcaacg cgccgtatgc
     1201 gtgttcggga aagcggtgga cgaacagggc aaaggcggct tcggatgtgc cgggcaggag
     1261 ggtggcggaa gtgaaggcgg aaaaagcgag cgcggcgtag gcgtaggcgg gcatcattgc
     1321 aaacagtctc aaacaggcaa aaatcggcga cggattgtac ggtatagtgg attaacaaaa
     1381 accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca
     1441 ccgagtgaat cggttccgta ctatctgtac tgtctgcggc tccgtcgcct tgtcctgatt
     1501 tttgttaatc cactatattt tcacgccccc gccgaagggc ggaggacggt gcaaaaaata
     1561 cggcacagcc gtatgcccct tttttgtcgg gcatacgaca ttctttccgc tccggttttg
     1621 atgccacgat gcggcatttc cgaattttcc ggatacggcg gcggattttc attttattgg
     1681 gaacggtttt tgcaagtccg ccggaatttt ttaaaatcta ttaaaatcta tgcaagcaac
     1741 tgtaaaatat taatttctgc tgcttgaatt tcagatcggc gcattgcctg catccgataa
     1801 agtttgcaaa atgttcaaat atcagtatga tttgcattgc cgttaagaaa tgtcaatttc
     1861 tattttcttg aaacgggtaa tattccgaca ccacgaaagg caaatcatgt ctgcgcaatc
     1921 acaaaacaat catacgtccc cattggtcgt cttgaccacg ctgttcttca tgatgggttt
     1981 tattacctgc atgaacgaca tccttatccc tcatttgaaa gaaattttcg acctgtctta
     2041 cgttcaggcg atgctgatcc aattctgttt ctttaccgcc tatgcggtga tgtccatccc
     2101 gatgggggct tttgtcggca aagtcggcta caaaaacggc gttatcggcg gctttctgct
     2161 gacggcggtc ggatgcctgc tgttttatcc tgctgcgggt agccattctt acgcggtatt
     2221 tttgggcgcg ttgtttattt tggcttccgg cgtaacgctg cttcaggtcg ccggtaatcc
     2281 ttatgttacc ctgctggcga aacccggcaa ggaatcggca acactgacgc tggttcaggc
     2341 gtttaacgct ttgggtacga ccattgcgcc gcaaatcggc gcgttcctga ttctggcgga
     2401 cgcaacccaa accgtcagca aggcggaaca gatttcttcc gtacagattc cctatttggg
     2461 actggcgggg ctgctgatta tccttgccgt tttcgtgaaa atgatccggc tgcccgacgc
     2521 gcgcaaaatt gccgccgagg aaagcgcgca caaccacgac ggcaaaacca gcgtatggca
     2581 atacaaacat ctcgtgttcg gtacggcagg cattttctgc tatgtcggcg cggaggtgtc
     2641 tatcggttcg ttgatggtca acgtattggg ttatctgaaa gggctggatc atgcttctgc
     2701 cgcgcattac ctgtcgttct attggggcgg cgcgatggtc ggacgtttcc tcggttcggc
     2761 ggtgatggcg aaattcgcgc ccaaccgtta tttggcgttt aacgcatcgg ctgcggtcgt
     2821 actgcttgcc gtcgcgatgg cgacgggtag cggcaatgcg gatgtggcga tgtggtcgct
     2881 gcttgccatc ggttttttca actcgattat gttcccgacg attttctctt tggcaaccaa
     2941 aggattggga aaatttacca acgcggcttc cggtgtactg tgtaccgcga ttgtcggcgg
     3001 tgcggtcgtt cctgtcgtgc agggctgggt ggcagatact tacaccctga tgtcttcgtt
     3061 tgtcgtttcc gtcatctgtt atctgtatat cgtgtttttt gcggtgtacg gatatagggc
     3121 ggacaaataa tctttttctt gagaaatgtc gtctgaacat ctttcagacg gcatttttgc
     3181 gtaccggtgt ttgcggcgtg tgtgccgagg ttttaatact tcaatccata aaagtcttat
     3241 atgtcaacaa acaaaaaaat aaaaaattat atttcaaaaa aattaattta aattgagaaa
     3301 attgccgttt tgtttctgtc cggcttttgt aaaacgctaa aatgccgtct gaaaacgtcg
     3361 ggcggattcg gtatggtgtg ttagaatccg ttaactttat atcaaatcgg gcaaagaatc
     3421 atgttcgctt tcaaatcctt actcgatatg ccgcgcggtg aggcacttgc cgtcgtcgtc
     3481 gctctgattg ccgcgatggg ctataccatc atttcattgg agtggctgcc gcatatgtcc
     3541 attattgccg ccatcgtcgt gctgattttg tacggcttgg cgcgcggttt gaaatacaac
     3601 gatatgcagc agggcatgat aggcgcgttg aatcagggta tgggcgcgat ttacctgttt
     3661 ttcttcatcg ggctgatggt cagcgcgctg atgatgagcg gcgcgattcc gacgctgatg
     3721 tattacggtt ttggactgat ttccccgact tatttttatt tttccgcctt cgcgctgtgt
     3781 tccgtcatcg gcgtgtccat cggcagcagc ctgaccacct gcgccactgt cggcgttgcc
     3841 tttatgggta tggcggcggc gtttcaggcc gatatggcga tgacggcggg cgcgattgtt
     3901 tcgggcgcat tttttggcga caaaatgtcc ccgctttccg atacgacggg catttccgcg
     3961 tccattgtcg gtatcgacct gtttgaacac atcaaaaata tgatgtacac caccattccc
     4021 gcgtggctca tcagtgcggc actgatgctg tggcttcttc ccagcgtcgc cgcgcaggat
     4081 ttgaacagcg tcgaatcctt ccgcagccag cttgaagcca cgggattggt gcactgctat
     4141 tcgctgattc cgtttgcgct gttggtcgtt ttggcattga tgcgcatcaa cgccgtcgtc
     4201 gccatgctct ttaccgtcat ggttgccgtt gctgtaacgt atctgcacag cacgcccgat
     4261 ctgcgtcagc tcggtgcgtg gttttacggc ggctacaaac tcgaaggcga agcgtttaaa
     4321 gatgttgtca aactgatttc gcgcggcggt ttggaaagta tgtttttcac gcaaaccatc
     4381 gtgattctcg ggatgagttt gggcggactg ttgtttgcgc tcggtgtgat tccttccctg
     4441 ttggaggcca tccgtacctt cttgacgaat gccggacgcg cgacgttcag cgttgccatg
     4501 acttcggtcg gggttaattt cctgattggc gaacaatatt tgagcatcct gctttctgga
     4561 gaaacgttta aacccgttta cgacaggctc ggactacatt cccgcaatct gtcgcggacg
     4621 ctggaagatg cggggacggt gattaacccg ctcgtaccgt ggagcgtatg cggcgtgttc
     4681 atcagccacg cgctgggcgt gccggtttgg gaatatctgc cgtatgcctt tttctgctat
     4741 ttgagtttgg ctttgaccct gttattcggt tggacggggc tgactttgag caaaaaataa
     4801 gcggataagc gaaatgccgt ctgaaacttg caacggtttc agacggcatt tttatgtttg
     4861 gcggatgggg cggattgaaa cagaaaacgc ccgtaccgtc atcctaaact gtgcagaaac
     4921 ggcagtgctt acttcacgcg ggtcgccatc agcgtgtgca ggcggcggtt gtcggcgcgg
     4981 gcgacggtga actgcaaacc gccgataagg actttttcgc cgcgtacggg caggtgtccc
     5041 aactcttgaa tgaccaagcc gccgatggta tcggcttctt cgctgctgta ttccgtgccg
     5101 aagaaggtgt tgatgtcttc gatttcggta gctgcatgga tgcgccagcg ttcggaagaa
     5161 acggcatgga tattgtcggc gctatcgtct tcgtcaaact cgtcttcgat ttcgccgacg
     5221 atttgctcga tgatgtcttc aaaggtgacc aagccggatg tgccgccgta ttcgtcgatg
     5281 acaatcgcca tatggttgcg ctgttcgcgg aactctttta aaagggcggt cagcgatttg
     5341 ccttcgggga cgaagacggc ggggcggaga atggatttga ggtggaactg ctcggggtta
     5401 aacatatatt tgagcaggtc tttggcgtgc aaaatgccca aaacttcgtc tttgtcttcg
     5461 ccgatgacgg ggaagcgcga atgggcggta tcgataacgt aggcggtgat gcgctcgatg
     5521 ctgtcgtttt cttttaaaac gttcatacgg ctgcgcgtaa tcatcgcgtc gcgcacttcc
     5581 aaatcggaaa aatcgaggac tttttccaac cttaaaagcg tatccgcatc aaatacttcc
     5641 tgctcgtgcg cctgccgaag caggtttaat acgtcttcgg cggaatcggg ttcgcgggcg
     5701 agtcgggcaa tcaggcgttc aaaaaaattt gttttcggtt gtgcgccgtc cattttaatg
     5761 tccgtcctct cggtaggggt tgggatagcc tgccgcccgc atcaggcgga tttcttcggc
     5821 ttccattatt tcggcttcat cgtcttcgat gtggtcgtag cccatcaggt gcaaaacacc
     5881 gtgtatggtc aggtgggcaa aatgccgttc gggcgttttg ccttgttcgg cggcttcttt
     5941 caaaacgact tgcgggcaga taatcaaatc gccgtacagt ttttccgaaa cttggcaggg
     6001 cagggtttcg ccttcgttga gcgcgaaact caagacattg gtggcgtagt ctttgccccg
     6061 gtagtcgcgg ttgtaggcgc gggctgcttc ttcgtccaga agaatcaggc tgatgtctgc
     6121 gcggcggtat ccgcttttca gggcagacca cgcccagcgg taaaaatcgt gttcgcttgg
     6181 aaggttggcg gcggaagagg cgttttcaaa gttcaaatgg aaacgttgcc gctgcaacgt
     6241 taagaaaggg tattttttgg tgcgtttcat tgtggcgggt ttcgtgtttt gtgggtgtaa
     6301 atataacata gaccacggca taccgtctga agaaacgttc aaaatatgat agactttacg
     6361 ccgtttccat tctttgaacg cattgaacat gaacccgaaa aaactcgtca tcgccagccg
     6421 cgaaagcctg cttgctatgt ggcaggcaaa gcatatccaa ggccgtctga aggctctgta
     6481 tcccgattgc gaagtcgaga ttttgggcat gaccacgcgc ggcgatcaga ttttggacaa
     6541 aactttgtca aaagtcggcg gtaaaggctt gtttgtcaaa gagttggaac aggctttata
     6601 tgacgggcgc gccgatttgg cggtgcattc gattaaagac gtaccgatgg atttgcctga
     6661 aggtttcgcg cttgcggcca tcggcgaacg cgccaatccg tttgacgcat ttgtgtccaa
     6721 ccaatacacg cgtttggaag aaatgcccga aggcgcggtt gtcggcacat ccagcctgcg
     6781 ccgcgaagcc cagttgcgtg cgcgctatcc gcatttggtt attaaacctt tgcgcggcaa
     6841 tgtgcaaacc cgtttgtcca aactcgataa cggcgaatac gacgcaatta tcttggctgc
     6901 cgccggtttg cagcgtctga aattggacgg acgcatccgc atgattttgt cggaatccga
     6961 cagcctgcct gccgccggac aaggcgcatt gggtatcgaa attgccgcgc accgcgaaga
     7021 tttgtatgaa gttttgaaac ccttgaacca cggcgttacc aatgcctgtg ttaccgccga
     7081 acgcgccctc gcacgcgctt tgggcggaag ctgccaagtg cctttggccg catattgcac
     7141 ggaagaaaac ggcttgctga ccttgcgcgg cttggtcgga caccccgacg gttcggttgt
     7201 gttgcgggcg gacgcgcaag cccctgccga atatgccgac gcgctcggac gcgctgtcgc
     7261 taagaaattg gcggacgacg gtgcgcggga attgattgga gcagtattga atacggaaaa
     7321 ttgattttat cgaaaattta aataaaataa tataagttat tgtttttaat caatttgttt
     7381 catcagtttt actcgcctt
//
LOCUS       DAXIHS010000088         7182 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXIHS010000088 DAXIHS010000000
VERSION     DAXIHS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.088"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            110..691
                     /locus_tag="ACFOF2_001745"
     CDS             110..691
                     /locus_tag="ACFOF2_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC7108015.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            760..942
                     /locus_tag="ACFOF2_001746"
     CDS             760..942
                     /locus_tag="ACFOF2_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC7108016.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            939..1700
                     /gene="kdsB"
                     /locus_tag="ACFOF2_001747"
     CDS             939..1700
                     /gene="kdsB"
                     /locus_tag="ACFOF2_001747"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC7108017.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            1723..2040
                     /locus_tag="ACFOF2_001748"
                     /pseudo
     CDS             1723..2040
                     /locus_tag="ACFOF2_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            2142..2342
                     /locus_tag="ACFOF2_001749"
                     /pseudo
     CDS             2142..2342
                     /locus_tag="ACFOF2_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            2499..2591
                     /locus_tag="ACFOF2_001750"
     tRNA            2499..2591
                     /locus_tag="ACFOF2_001750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2533..2535,aa:Ser,seq:gct)
     gene            2894..3679
                     /gene="trpA"
                     /locus_tag="ACFOF2_001751"
     CDS             2894..3679
                     /gene="trpA"
                     /locus_tag="ACFOF2_001751"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC7108018.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            3716..4588
                     /gene="accD"
                     /locus_tag="ACFOF2_001752"
     CDS             3716..4588
                     /gene="accD"
                     /locus_tag="ACFOF2_001752"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC7108019.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(4648..5259)
                     /locus_tag="ACFOF2_001753"
     CDS             complement(4648..5259)
                     /locus_tag="ACFOF2_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC7108020.1"
                     /translation="MRRTALLLLALAAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIIPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            5368..5592
                     /locus_tag="ACFOF2_001754"
     CDS             5368..5592
                     /locus_tag="ACFOF2_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC7108021.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGVPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5593..5739
                     /locus_tag="ACFOF2_001755"
     CDS             5593..5739
                     /locus_tag="ACFOF2_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108022.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            5955..6989
                     /gene="pyrC"
                     /locus_tag="ACFOF2_001756"
     CDS             5955..6989
                     /gene="pyrC"
                     /locus_tag="ACFOF2_001756"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC7108023.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
BASE COUNT         1721 a         1830 c         1972 g         1659 t
ORIGIN      
        1 atgcctagga ggcttcagaa atcccaaatc tccggatttc cacttggaca ggaatgagaa
       61 aatcggtcgt attttttatc tgcattaatc attcgttaaa gtattgaata ttaaactgaa
      121 aaccttgtta ttgcccttcg ccacgctggc attgtgcacc aacgcttttg ccgccccgcc
      181 tagcgacgcg tcgttggcgc gttggctgga tacgcagaat tttgaccggg atatagaaaa
      241 aaatatgatt gagggcttta atgccggatt taaaccgtat gcggacaaag cccttgccga
      301 aatgccggaa gcgaaaaaag atcaggcggc agaagccttt aaccgttatc gtgagaatgt
      361 tttgaaagat ttgattacgc ccgaagtgaa acaggctgtc cgtaatactt tattgaagaa
      421 tgcccgtgag atatacacgc aagaagaaat tgacggcatg attgcctttt acggttcgcc
      481 tgtcggtcag tccgtcgttg ccaaaaatcc gcgcttaatc aagaaatcga tgagtgaaat
      541 agcagtatct tggactgcat tgtcagggaa aatcgcgcga catcatctgc ccgcgtttac
      601 ggaagagttg cggcgcatca tctgcggcgg taaaaatccc gatgcgggct gtaaacaagc
      661 cggacaggtt gggaaaaggc atcagaaata aatgatagcc gtctgaaata ttgaagaggg
      721 catccgattg attgaaccat caaacccgaa agcaacccta tggaaaaaaa attcttagac
      781 atcctcgtct gccccgttac caaaggcagg ctggaatatc atcaggacaa acaggaattg
      841 tggagccgtc aggcgaagct tgcctatccg attaaagacg gcattcccta tatgctggaa
      901 aacgaagcgc gtccgttggg cgaagaggaa ctcgaagcat gaccgaattc gtcgtattga
      961 ttccggcgcg gctggattcg tcgcgcctgc ccggaaaagc cttggcggac atccacggca
     1021 aaccgatggt cgtgcgcgtt gccgaacagg cggcaaaaag taaagctgtg cgcgtcgtcg
     1081 ttgccaccga ccatcccgat attcagacgg cctgtcaggc gcacggcgtc gaagtcgtca
     1141 tgacttcaaa ccggcacgaa agcggcacga cgcgccttgc cgaagcctct gccgcgctga
     1201 agctgccgcc gcatctggtg gttgtcaacg tacagggcga cgagccgctg attgcacctg
     1261 aactcatcga ccgcaccgcc gaagtactcg tcgaaaacaa cgtccaaatg gcgaccgccg
     1321 cccacgaatt gcacgatttc gacgaattga tgaatcccaa tgccgtcaaa gttgtcctcg
     1381 acaaaaaccg caacgccatc tacttcagcc gcgccccgat tccctatccg cgcgatgcga
     1441 tgcgtgcagg aaaacgcgaa atgccgtttg aaaccgccgt cctgcgccat atcggcatct
     1501 acgcctaccg cgtcggcttc ctgcaacgct acaccgaaat gagcgtttcg ccgctggaaa
     1561 ccattgaatc gctggagcaa ctgcgtgtcc tgtggcacgg ctacccgatt gccgtcgaaa
     1621 ccgccaaaga agctcctgcc gccggcgtag atacgcagga ggatttggac aggattcgcg
     1681 ctgtatttca gactatataa aatagattca aagggaaaag atatgcagca acatattgaa
     1741 aaatggcaac acttgagccg ggaagaacag aaaatccttg ctgaagtatg gggtctcgtg
     1801 caaaacgacg atcaggaggt tcactatgaa atgctcaaat tgaacgcacc cgacgaagcc
     1861 agtggcgaat tttggttcag aatggcagaa acactcagca ccctgccgcc caaccgttcc
     1921 ctcgacctta gaatgaacgg cggcaggctg gcgaccgccg tatccatcct ttccgtcatg
     1981 attgaagaca atcccgacat accgcagctt tgggcgcaaa aaattaccgc gctcaattat
     2041 agtggattaa atttaaacca gtacggcgtt gcctcgcctt gtcgtactat ctgtactgtc
     2101 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatttggca cacggacaca
     2161 aagcccgtgc cgacggtttg gcacaacagc ccgacaaagc ggcagaagcc aacgaggagg
     2221 aatacctgac caaagccctg tcgcaaaacc tactgtcaac attggatgcc gcgcttgcac
     2281 gttttcctga agacgcgtgg tttcaggaaa tcaaacagga tgcacaaaag cattttgctt
     2341 gaggatgtgg cagtcaggaa tatttccatt caggaagaaa agaagtgcct gattgggtat
     2401 aatcagggta aatcttattt tatttcaaaa gattaatatt tgctttctgt ttttccttga
     2461 cggtatcgga aaagttgatt atagttacag cttccttagg agtaatggct gagaggctga
     2521 aggcacttcc ctgctaagga agcatgtggg gtcaacctgc atcgagggtt cgaatccctc
     2581 ttactccgcc aagatgagaa gacgctgtgt tttacagcgt ctttttttac gcgctttgtt
     2641 gcggaagtgc cgagggctgt ctgccgtgcg ccgcctggat ttgcaatgtg cccgcccgtg
     2701 caaaatccga tttgaccaac ctgaagggca ggtttcggaa gagcggggaa ttttgataaa
     2761 caaaaaactg cacggcataa tctttcccgg ttttcatttt ccgtattcgc ctatccgtac
     2821 cgcaaatgct atactgggaa aaatttaccg attgtgtttt acggcatatt tgccgatagg
     2881 atggaagaga caaatgagca gaatccggca ggcttttgcc gctttaaacg gtgaaaaggc
     2941 attgattcct tatattacgg ttggagaccc ggacattggg gcgactttgg caataatgca
     3001 cagtatggtg gaaaacggtg cggatatttt ggagttgggc gtgccgtttt ccgatccgat
     3061 ggcggatgga ccggttattc agcgtgcggc ggagcgggcg ttggcaaacg ggatttcgct
     3121 gcgcgatgtc ttggatgtcg tcagaaaatt ccgtgaaacc gacacgcaaa cgccggttgt
     3181 tttgatggga tatttgaacc ctgtacataa gatgggttat cgggagtttg ctcaggaagc
     3241 cgcaatggcg ggtgtggacg gcgtgttgac ggtggattcc cctgtcgaaa ccatcgatcc
     3301 gctctatcgc gagctgaagg ataacggggt cgactgtatt ttcctgattg cgccgacgac
     3361 gacggaagac cgtattaaaa ccattgccga gctggcaggc ggatttgtct attatgtttc
     3421 gctcaagggc gtaacgggcg cggcaagttt ggatacggat gaggtttcgc gtaaaataga
     3481 gtatttgcat cagtatatcg atattcccat cggtgtcggt ttcggcatca gcaatgcgga
     3541 aagcgcgcgc aaaatcggcc gggttgccga cgcggtcatt gtcggcagcc ggattgtgaa
     3601 agagattgaa aacaatgcgg gcaacgaggc tgcggtcgtc ggtgctttgg tcaaagagtt
     3661 gaaggatgcc gtgcgctgat tctgtcacgc attctaaata ttttaggagt tgtccatgag
     3721 ctggttagat aaaatcctgc cacccaaaat caaaaaccgc agcaaagacg gttcttccaa
     3781 tgttcccgag ggtttgtggc acaaatgtcc gtcttgttcg gcaaccgttt attcgaccga
     3841 gttgcagcag aacaatcagg tttgtccgaa atgcaaccac cacaatccgt tgtcggcacg
     3901 acaacgcctg aacctgcttt tggatgagga tggcagggag gaagttgccg gaaatgtcaa
     3961 accgatagat cctttgaagt ttaaagacag taaaaaatat cccgaccgtt tgggggcggc
     4021 acgcaagctg accggggaag atgacgcgct ggtggtgatg aaggggatga tgaacggcct
     4081 gcccgtcgtc gttgctgcgt ttgaattccg ctttatcggc ggttcgatgg gttcggttgt
     4141 gggagagcgt ttcgtacagg gcgtccgccg tgcggttgcc gacaattgtc cgttcgtctg
     4201 tgtggcggct tccggcggtg cgcgtatgca ggagggtgtg aactcgttga tgcagatgac
     4261 gaaaaccagt gccgcgctgc atttgctgac ggaaaaacgc ctgccgttta tatcggtatt
     4321 gaccgatccg actatgggcg gcgtatccgc cagcttcgca tttttgggtg atgttgtgct
     4381 tgccgaaccg aacgcgctga tcggttttgc cggtccgcgc gtgattgagc agacggtgcg
     4441 cgaaacgctg ccggaaggct tccaacgcgc cgagttcctg ctggaaaaag gcgcaatcga
     4501 ccagattgtc gaccgccgcg atatgaagcg gcgcatcagt gatttgatta cgctgttgtg
     4561 ccgtcaggac aaagtttccg ccgcttgatg gctgatgaat cgaataccgt ctgaaaccga
     4621 tgtttcagac ggtatttttg tgtctggtta tttgttgtgc ggcttcatcg acggggcata
     4681 gcgtccggca cgttctttca ggcgttgtac caaacctttc gtgtcggcgg gtacaccgcc
     4741 ctcgcagaat gcctgataca ggacggtgcg cagtgcgtcg ttgcggctta atgtaccgcc
     4801 tatcggtttc cattcggcgt ttcggggctg tatccagcgg cggttgaccg tgtcgccgta
     4861 gccgaacact ttataggagg aaggtttgcc gttgccgaac ctgatggaga agcgggcgca
     4921 gaatatgcct tcggcagtca ggttgtcgta gcctttgtcg gagcggatat tgagaatgta
     4981 gcggatgctg ccgtcgggcg cgggtataat ttgcaggctg tcgagcagga ttttcggctg
     5041 tttgccgtaa ttttcatcca cataaatgtc aaaccagccg tccgagtgcg tatcgggcag
     5101 cggcggcagt ttggcggtat gttctttaaa ttcgcgggcg gcggcttctt cgggcgtttc
     5161 gcggtagcgg gtgttgatcg gcgtgtcttt ttggctgaag ctggtggcaa gggacgtgcc
     5221 ggcggcgagt gcgagcagca ggagggcggt gcggcgcata agtttctcca aattgaaaac
     5281 ggcgttattt tatgggttgg caaagggggc tgcaagcaac tggggtataa tctccccccg
     5341 attcccattt tttaaacggt acaaacgatg aacagcgaaa ctttagacgt aaccggattg
     5401 aaatgtcccc tgccgatttt gcgggcgaaa aaggctttgg cgcaaatgca gcagggcgac
     5461 gtgttgaccg ttctggcaac cgacggcggc gtaccggggg attttgaggc tttttgccgc
     5521 caaaccggtc atgtgctgtt ggatgcttcc gaacaggacg gcgtgttcac gctggtcgtc
     5581 caacacaaat aaatgccgtc tgaaatgcgg atgtcccgcc cgacggcgtt gtttttgaat
     5641 atcgtatgtg ccgcgtgccg tttcaaaaac aaaccgtccg gcgtgcagtg tcggcatttc
     5701 gggcgtatcg ccgccgattt gttccggaat ggctgttaac cgccttgccg tcggcaaaga
     5761 agcaaaaccc aagcacaatc aaaatctaaa ggctgtgttt gaagattccg ttgatgcaac
     5821 ccaatcagtc ggacaaaatg cctttcatcc aatggaaccg gtttccgacc ggacggaata
     5881 accggctttc ccccggcaaa cggatggaat cgaccgggta ttcaaacgca gccaaaacct
     5941 aaaaaggaac aaccatgcaa accctgacca ttatccgccc cgacgatatg cacctgcacc
     6001 tgcgcgacgg cgacgcgctc aaagccgttg ctccttatac cgcccgccaa atggggcgcg
     6061 ccgtcatcat gcccaacctc aaaccgcccg tcgtcagcgt agccgatgcg cttgcctaca
     6121 aagcgcgtat tatggcggcg ttgcccgaag gcagcgcgtt tgagccgctg atgacgcttt
     6181 atttgaccga caacgccacg cctgcacttg tgcgcgaagc caaagccgcc ggcatcgtcg
     6241 ccttcaaact ctaccctgcc ggcgcgacca ccaattccga ttccggcgta accgacctgt
     6301 tcaagctcat ccccgtgttg gaagaaatgg cgaaacaggg cattttgttc ctcgttcacg
     6361 gcgaagtaac cgaccccgaa atcgacatct tcgaccgcga agccgccttt atcgggcgcg
     6421 tgatgaaacc cgttttggcg caagtgccga atcttaaagt cgtgttcgaa cacatcacca
     6481 ccgccgaagc cgcccgcctg gttttggaag caggcgacaa cgtagccgcc accgttaccc
     6541 cgcaacacct cctgctcaac cgcaacgacc tcttggtcgg cggcgtgcgc ccccatcatt
     6601 tctgcctgcc cgtactcaaa cgcgaaaccc accgtcaggc attggttgcc gccgttaccg
     6661 gcgagaaggc gcataaattc ttcctcggca ccgactccgc gccgcacgcc aaatccgcca
     6721 aagaaaacgc ctgcggttgc gccggtatgt tcagcgcgat gaccgccatc gagctttatg
     6781 ccgaagtgtt tgaaaaagca ggcgcgttgg acaaactcga agccttcgcc tcgaaaaacg
     6841 gcgcaaggtt ttacggcatt cctgaaaata ccgacacgat caccctcgtc aaacaaagcc
     6901 aaaccgtccc cgcaagtatt ccctacggcg acggcgaact tgtcccgatg cgcgcgggcg
     6961 gcgaaatcgg ctggacggta cagtattgat ggggctggaa acaaaatgcc gtctgaaagg
     7021 tttttcagac ggcatttgtg ttgttgactg attgatacgt caacggcgtt tgagtttgtt
     7081 acgtttcggt tatttccgat aaattcccac aattttcaaa tttcgccatt cccacgaagg
     7141 caggaatccg gaaattcgat gcgaccagag tttatcaaaa ac
//
LOCUS       DAXIHS010000089         7170 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXIHS010000089 DAXIHS010000000
VERSION     DAXIHS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.089"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            21..224
                     /locus_tag="ACFOF2_001757"
     CDS             21..224
                     /locus_tag="ACFOF2_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108024.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            251..1213
                     /locus_tag="ACFOF2_001758"
     CDS             251..1213
                     /locus_tag="ACFOF2_001758"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC7108025.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            1260..1631
                     /locus_tag="ACFOF2_001759"
     CDS             1260..1631
                     /locus_tag="ACFOF2_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC7108026.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            1657..2583
                     /gene="fabD"
                     /locus_tag="ACFOF2_001760"
     CDS             1657..2583
                     /gene="fabD"
                     /locus_tag="ACFOF2_001760"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC7108027.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDGQVKAICAESAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQIAAFIEAH"
     gene            2722..4587
                     /gene="msbA"
                     /locus_tag="ACFOF2_001761"
     CDS             2722..4587
                     /gene="msbA"
                     /locus_tag="ACFOF2_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC7108028.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            4687..6252
                     /gene="guaA"
                     /locus_tag="ACFOF2_001762"
     CDS             4687..6252
                     /gene="guaA"
                     /locus_tag="ACFOF2_001762"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC7108029.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            6314..7060
                     /gene="fabG"
                     /locus_tag="ACFOF2_001763"
     CDS             6314..7060
                     /gene="fabG"
                     /locus_tag="ACFOF2_001763"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC7108030.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1717 a         2264 c         1779 g         1410 t
ORIGIN      
        1 atggaaaccc aacaatcaaa atgccgtctg aagcagcttg ggctttcaga cggcatttcc
       61 tccgcttaaa acagcatatc ggcaaccccg ccccgcccgt ccacggcaat ctgcatctga
      121 aaaccatctg tatcccaaac cacaccccca tcccgatttc cgtcatttgc accctgcccg
      181 tattgggcaa tcatcggttt ttcgcttaca atagccgaat ctgaaccaac tatccaaaaa
      241 ggccgttccc atgcagtatg ccaaaatttc cggcacgggc agctatcttc ccgccaaccg
      301 cgtcagcaat gacgaccttg cccaaaaagt ggatacctcg gacgagtgga ttaccgcgcg
      361 cacgggcatc aaattccgcc atattgcagc cgaaaatgag aaaaccagcg atcttgccgc
      421 cgaagcagcc cgccgcgcac tggatgcagc cggattagac agcggcgaaa tcgatttaat
      481 tattgtggcg acggcaacgc cggatatgca gtttccttct accgccacca tcgtccaaca
      541 aaaactgggc atcaccaacg gctgccccgc gtttgacgta caggcggtgt gtgcgggctt
      601 tatgtacgcg ctgaccacgg caaacgccta catcaaaagc ggcatggcaa aaaacgcgct
      661 ggtcatcggc gcggaaactt tcagccgcat cgtcgattgg aacgaccgca caacctgcgt
      721 attgttcggc gacggcgcgg gcgcggtggt tttgagcgcg gcggacaaac cgggcatcat
      781 ccacagcaaa ctcaaagccg acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat
      841 cgcctgcggc aaagtttccg gttcgccgta catttcgatg gacggtcccg gcgtgttcaa
      901 gtttgccgtc aaaatgctgt ccaaaatcgc cgatgacgtt atcgaagaag ccggttatac
      961 cgccgctcaa atcgactgga tcgtgccgca tcaggcaaac cgccgcatta tcgaatcgac
     1021 cgcgaaacat ttaggtttga gtatggacaa agtcgtcctg accgtccaag accacggcaa
     1081 cacatccgcc gcatcgattc cgctggcttt ggatacgggc atccgcagcg gacaaatcaa
     1141 acgcggtcaa aacctgctgc tcgaaggcat cggcggcggt ttcgcgtggg gcgcggtgct
     1201 gttgcaatat tgaacccgat gccgtctgaa acaggctttc agacggcatt tcccatatca
     1261 tgaagcggca ggctttcttc aaaccaattg cgtgtgcggc atttctgtct gccgtttcgc
     1321 tgcgcctccc cgtattgggc gcgtgttacg caatattgtc cctctatgcg tttgcacttt
     1381 acggcatcga caaacggcgt gccgtgcggg gaaaacgccg cattcccgaa caccgcctgc
     1441 tgctgcctgc cttgttcggc ggttgggcgg gcgcatactt gggcagcagg atattcaggc
     1501 ataaaacggc gaaaaagcgt tttgttgtgc tgttccgtct gactgtttcg ggcaatgtcc
     1561 tggcgacctg catcctgatt gattatttcg ttccgcccga actttttgtc aaactcgggc
     1621 aacatctctg acaatccaac caaacaaagg aataccatgt cttttgcatt cttctttccc
     1681 ggacaaggtt ctcaaagcct cggcatgatg aacggcttta cagggcaggc cgtcgtcaaa
     1741 aacacatttg acgaagcctc cgccatattg gggcaggact tgtgggcgat gattaacggc
     1801 agcgatgccg aaatcatcgg tcaaaccgtc aatacccaac ccatcatgct cgccgccggc
     1861 gttgccgttt accgcgccta tttggaagcg ggtggcaaaa cgcctgccgc cgttgccgga
     1921 cacagcctcg gcgaatatac cgcgcttgtc gccgccggcg cattggattt tgccaacgcg
     1981 gtcaaactcg tgcgcctacg cgcagaactg atgcagtccg ccgtaccgca aggcgtgggc
     2041 gcaatggcgg cgattctcgg cttggaagat gggcaggtta aagccatttg tgccgaatcc
     2101 gcacagggcg aagtggtcga agccgtcaac ttcaactcgc ccgggcaaat cgtgattgca
     2161 ggcaacgccg ccgccgtcgg acgcgccatg gctgccgcca aagaagccgg agccaaacgc
     2221 gccctgccgc tgcccgtgtc cgtaccttcc cattgcagcc tcatgaaacc cgccgccgac
     2281 aaacttgccg aagccttgaa aaccgttgaa atcaagcagc cgcaaatccg cgttatccac
     2341 aacgccgacg ttgccgccta cgatgatgcc gacaaaatca aagacgcgct cgtccgccag
     2401 ctttacagcc ccgtacgctg gacggaaacc gtcaacgccc tcgtttcaga cggcattgcc
     2461 gaatccgccg aatgcggccc gggcaaagtt ctggcaggtc tggcaaaacg catcaacaaa
     2521 gccgccgcgt gcagcgcact gaccgatgcc ggacagattg ccgcctttat agaagcacac
     2581 tgacttcgtt ctgtaaaaag cagcctgcat gcctcaggct gcttttcacg tccgaacggc
     2641 aaacagccgc atatttgcgc tataattgat gacgaccaaa ccaccgacag cggctgccgt
     2701 tgcagttccc gccccaccga catgatagaa aaactgactt tcggactgtt taaaaaagaa
     2761 gacgcgcgca gctttatgcg cctgatggcg tacgtccgcc cctacaaaat ccgcatcgtt
     2821 gccgccctga ttgccatttt cggcgttgcc gccaccgaaa gctaccttgc cgccttcatc
     2881 gcccccctga ttaaccacgg cttttccgca cctgccgcgc cgcccgagct gtctgccgcc
     2941 gccggcatca tttccaccct gcaaaactgg cgcgaacagt ttacctatat ggtttggggg
     3001 acggaaaaca aaatctggac cgtcccgctc tttctcatca ttctcgtcgt catccgtggc
     3061 atctgccgct ttaccagcac ctatctgatg acttgggtct ccgtgatgac catcagcaaa
     3121 atccgcaaag atatgtttgc caaaatgctg accctttcct cccgctacca tcaggaaacg
     3181 ccgtccggca ccgtactgat gaatatgctc aacctgaccg aacagtcggt cagtaacgcc
     3241 agcgacatct tcaccgtcct cacgcgcgac acgatgatcg ttaccggcct gaccatcgtc
     3301 ctgctttacc tcaactggca gctcagcctc atcgttgtcc tgatgttccc cctgctctcc
     3361 ctgctctcgc gctactaccg cgaccgtctg aagcacgtca tttccgactc gcaaaaaagc
     3421 ataggcacga tgaacaacgt gattgccgaa acccatcagg gacaccgcgt cgtcaagctg
     3481 ttcaacgggc aggcgcaggc ggcaaaccgg tttgacgcgg tcaaccgcac catcgtccgc
     3541 ctcagcaaaa aaatcacgca ggcaacggcg gcacattccc cgttcagcga actgattgcc
     3601 tcgattgccc tcgccgttgt catcttcatc gccctgtggc aaagccaaaa cggctacacc
     3661 accatcggcg aatttatggc attcatcgtc gcgatgctgc aaatgtacgc acccatcaaa
     3721 agccttgcca acatcagcat ccctatgcag acgatgttcc tcgccgccga cggcgtatgt
     3781 gcatttctcg acaccccgcc cgaacaggac aaaggcacgc tcgcgccgca gcgtgtcgaa
     3841 gggcgtatca gcttccgcaa cgtcgatgtc gaataccgtt cggacggcat caaagccctc
     3901 gacaacttca acctcgacat cagacaaggc gaacgcgtcg ccctggtcgg acgttccggc
     3961 agcggcaaat ccaccgtcgt caacctgctg ccccgctttg tcgaaccgtc tgccggcaac
     4021 atctgcatag acggtatcga catcgccgac atcaaactcg actgcctgcg cgcccaattc
     4081 gccctcgtct cccaagacgt attcctgttt gacgacaccc tgtttgaaaa cgtccgatac
     4141 agccgtcccg acgcaggcga agccgaagtc ctgtccgccc tccaagccgc caacctgcaa
     4201 agcctgattg acagctcccc gctcggactg caccagccca tcggatcgaa cggcagcaac
     4261 ttgtccggcg gacagcggca acgcgtcgcc atcgcccgcg ccattttgaa agacgcgccg
     4321 atattattat tggacgaagc caccagcgca ttagacaacg aatccgaacg cctcgtccaa
     4381 caagcgctcg aacgcctgat ggaaaaccgc accggcatca tcgtcgccca ccgcctgacc
     4441 accatcgaag gggccgaccg catcatcgta atggacgacg gcaaaatcat cgaacaaggc
     4501 acacacgaac aactgatgtc ccaaaacggc tactacacga tgttacgcaa tatctcaaac
     4561 aaagatgccg ccgtccggac ggcataaaca aaatgccgtc cgaaatggta taatcgcccc
     4621 gaccctttca gacggcacta tatccgccga cccatccgat tatcttcaat cactgtaaaa
     4681 cccattatga cccaagacaa aatcctcatc ctcgacttcg gttcgcaagt tacccagctg
     4741 attgcccgcc gcgtgcgcga agcccacgtt tactgcgaac tgcattcctt cgatatgcct
     4801 ttggacgaaa tcaaagcctt caaccccaaa ggcatcatcc tttccggcgg ccctaattcc
     4861 gtttacgaat ccgactatca ggccgatacc ggtatttttg atttgggcat tccggttttg
     4921 ggcatctgct acggaatgca gtttatggcg caccacttgg gcggcgaagt gcagcccggc
     4981 aaccagcgcg aattcggtta cgcgcaagtc aaaaccatcg acagcgaact gacccgcgat
     5041 attttcgacg acgcgcccaa cacactcgac gtatggatga gccacggcga caaagtgtcc
     5101 aaactgcccg acggcttcgc cgtcatcggc aacaccccgt cctgcccgat tgccatgatg
     5161 gaaaacgccg aaaaacaatt ctacggcatc cagttccacc ccgaagttac ccacaccaaa
     5221 caaggccgcg ccctgttgaa ccgctttgtc ttggatattt gcggcgcgca accgggctgg
     5281 acgatgccga actacatcga agaagccgtt gccaaaatcc gcgaacaggt cggcagcgac
     5341 gaagtgattt taggtttgtc aggcggcgta gactcttccg tagccgccgc gctgattcac
     5401 cgcgccatcg gcgaccaact gacctgcgtg ttcgtcgatc acggtttgtt gcgcctgaac
     5461 gaaggcaaaa tggtgatgga tatgttcgcc cgcaacttgg gtgtgaaagt gattcacgtc
     5521 gatgccgaag ggcagtttat ggcgaaactc gccggcgtta ccgaccctga gaaaaaacgc
     5581 aaaatcatcg gcgcggaatt tatcgaagta tttgatgcca aagagaaaaa actcaccaac
     5641 gccaaatggc tggcgcaagg cacgatttac cccgacgtaa tcgaatccgc aggcgcgaaa
     5701 acccaaaaag cccacgccat caaatcgcac cacaacgtcg gcggtctgcc cgaaaatatg
     5761 aagctcaagc tgcttgagcc gttgcgcgac ttgtttaaag acgaagtgcg cgaactgggc
     5821 gttgctttgg gtctgccgcg cgaaatggtg taccgccatc cgttccccgg ccccggtttg
     5881 ggcgtgcgca tcttgggcga agtgaaaaaa gaatacgccg acttattgcg tcaagcagac
     5941 gatattttta ttcaagaatt acgcaacacc accgacgaaa acggcacgtc ttggtacgac
     6001 ctgaccagcc aagccttcgc cgtgttcctg cctgtgaaat ccgtcggcgt aatgggcgac
     6061 ggacgcactt acgactacgt cgtcgcactg cgcgcagtca tcaccagcga ctttatgacc
     6121 gcacattggg ctgagttgcc ttactcactg ctcggacgcg tgtccaaccg catcatcaac
     6181 gaagtcaaag gcatcaaccg cgtggtgtac gatgtcagcg gcaaaccgcc tgctaccatc
     6241 gagtgggaat aaccagcaaa cagggctgca ccgtccggcg cagtctttcg attatcggaa
     6301 aaaaggaaaa aatatgagca cacaagattt aagcggcaaa atcgctttgg taacaggcgc
     6361 ctcgcgcggt atcggtgcag caattgccga cacactggcg gcagccggtg ccaaagtcat
     6421 cggtacggcg accagtgaga gcggtgcggc ggcgattagc gagcggttgg cgcaatgggg
     6481 cggcgaaggc cgcgtattaa attccgccga acccgaaacc gtcgaaaacc tgattgccga
     6541 catcgaaaaa acgttcggca aactcgatat tctggtcaac aacgccggca tcacccgcga
     6601 caacctcctg atgcgtatga aagaagaaga gtgggacgac atcatgcagg tcaacctcaa
     6661 atccgtcttt cgcgcctcca aagccgtctt gcgcggcatg atgaagcagc gcgccggccg
     6721 tatcatcaac atcacatccg tcgtcggcgt gatgggcaat gccggtcaaa ccaactatgc
     6781 cgcggcaaaa gcaggcttaa tcggtttctc caaatccatg gcgcgcgaag tcggcagccg
     6841 gggcattacc gtcaactgcg tcgcccccgg ctttatcgat accgacatga cccgcgccct
     6901 gccggaagaa acccgccaaa cctttaccgc ccaaaccgcc ttgggcagat tcggcgacgc
     6961 gcaagacatt gccgatgcag tcttgttctt ggcttccgat caggcaaaat acattaccgg
     7021 ccagacgctg cacgtcaacg gcggcatgct gatgccctga cggacagccg tgccgacaat
     7081 gccgtctgaa gccctttcag acggcatttg cattcccaaa caaaatgaac acacgccaca
     7141 ccccgccctg cccctgcgga tcaggcacaa
//
LOCUS       DAXIHS010000090         7109 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXIHS010000090 DAXIHS010000000
VERSION     DAXIHS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.090"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(129..2336)
                     /gene="uvrD"
                     /locus_tag="ACFOF2_001764"
     CDS             complement(129..2336)
                     /gene="uvrD"
                     /locus_tag="ACFOF2_001764"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC7108031.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            2520..3167
                     /gene="pxpB"
                     /locus_tag="ACFOF2_001765"
     CDS             2520..3167
                     /gene="pxpB"
                     /locus_tag="ACFOF2_001765"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC7108032.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            3164..4093
                     /locus_tag="ACFOF2_001766"
     CDS             3164..4093
                     /locus_tag="ACFOF2_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC7108033.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            4080..4817
                     /gene="pxpA"
                     /locus_tag="ACFOF2_001767"
     CDS             4080..4817
                     /gene="pxpA"
                     /locus_tag="ACFOF2_001767"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC7108034.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            4946..6139
                     /locus_tag="ACFOF2_001768"
     CDS             4946..6139
                     /locus_tag="ACFOF2_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC7108035.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            6247..6939
                     /locus_tag="ACFOF2_001769"
     CDS             6247..6939
                     /locus_tag="ACFOF2_001769"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC7108036.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
BASE COUNT         1478 a         1852 c         2117 g         1662 t
ORIGIN      
        1 gtccttgcta cagttttttg ccataggtcg gattctcgaa tccgacattt tccaacagcg
       61 gtttttcgga aacgatagac gcgtcaaata tttttgtcgg atacgagtat ccagcctgca
      121 tttcaaattt acatctcttc caatttcgca aacttggtgt ccaactcttt cacgccctgt
      181 ttgccgaaat tgatggtcag tcgggcggat tcgcctttat ctgcggcatc gataatcacg
      241 ccggtgccaa atttggcgtg gcggacgttt tgtccgatac ggaaacctgc gtaggtttgg
      301 ggctgtttgt agtcgtcgat gattttgtct ttggatgcgg cggtttggcg cgggctgccg
      361 tagctgtcgt aggcggtctt tttgacggac aggtagtgca atacttcggg tgggatctct
      421 tcgacgaagc gggagacgat gccgaattgg gtttgtccgt gcagcatgcg ttgttgcgcc
      481 atggtgatgt agaggcgttt gcgggcgcgg gtgatggcga cgtacatgag gcggcgttct
      541 tcttcgaggc cgccgcgttc ggcaaggctc atttcgctgg ggaagcggcc ttcttccatg
      601 ccggtgagga agacggcgtt gaattccaag cctttggcgg cgtggacggt catcagttgg
      661 acggcttttt cgcctgcgcc tgcttggttt tcgccggatt cgagggcggc gttgctcaag
      721 aaagcgagga tggggaaggc ggggtcgtct gaaatgttgt cgggcagggt ttcaaagttg
      781 ctgtcttcgg gtttgaactc gatggcggcg ttgacgagtt cgtcaaggtt gtcgaggcgg
      841 tcttggttgt cgcctttttg ggtgcggtag tgttcggtca agccactgtc tttgaggatg
      901 ccggcgatga tttcgggcag gggcatttgt ccgacttggt tgcgcagggc ttcaatgagg
      961 cggacgaagg cggcgacttt ggcggctttt gcgccggcat tgcaggctgc ctgccaaagt
     1021 gaaacgcctt gctcttgcga tgccgtctga aggttttcga cggtgcgtgc gccgatgccg
     1081 cgcggtggga agttaatcac gcgcaagagg gcgttgtcgt cgtcgggatt gacggcgagg
     1141 cgcagatagg caagcgcgtg tttgatttct tggcgttcgt aaaagcgcaa gccgccgtag
     1201 attttgtagg ggatgccgct gcggaacagg ctttgttcga taacgcggga ttgggcgttg
     1261 ctacggtaga ggacggcgat ttcgtccaaa tcccagcctt cgcgttcgag ggctttggtt
     1321 tcgtccaaga tgaaccgggc ttcttcgagg tcggtaaagg cggagtagta gcggattttg
     1381 tcgcctgctt cggcgtcggt gcgcaggttt ttgccgagtc gttcgtcgtt gttttcaatc
     1441 acggcattgg cggcggcaag gatgttgccg acggagcggt agttttgttc gagtttgacg
     1501 ggcgcgtcga tgtggaattc ttccatcagc gcggtcatgt tgccgacgtt tgcgccacgg
     1561 aaacggtaaa tgctttggtc gtcgtcgccg acggcaaata ccgctgcgtt tccgcccgcc
     1621 atgagtttga gccaggcgta ttgcagtttg ttggtgtctt ggaactcgtc gacgagaatg
     1681 tggttgaagc ggttttggta atgctggcgc aggatttcgt tgctttgcag catttcgtag
     1741 ctgcggagca tgagttcggc aaaatcgacc acgccttcgc gttggcagat tttgtcgtat
     1801 tcggcgtagc attcaatcat gcggcttgtg tgcggatcgg gcgcactcaa gacggaagcg
     1861 cgcaaaccgg attctttttg cgcgttgata aagccttgca gcgaacgcgg cgcgatgatt
     1921 tcttcggcga tgttgaggct tttgagcagg cgtttgatga gggaaagctg gtcgccgccg
     1981 tcgaggattt gaaaggaaga cggcagaccg gcgtcgcggt ggtgcaggcg caaaaagcgg
     2041 tggcagaggc cgtggaacgt gccgagccac atggcgcgga cattgatggg aatcatcgcg
     2101 cccaaacggg tttgcatttc tttggcggct ttgttggtaa acgttaccgc cataatgctg
     2161 tgcacgcttg cctgaccggt ttgcaacagc catgcgatgc gcgtggtcag cacgcgcgtt
     2221 ttgccgctgc ccgcgcccgc cagcacaagt gcggattgcg gcggccaggt tacggcggag
     2281 agttgttcgg gattcaagcc ttgcagcagg ttgggggcgg attggtcggg aaacataagg
     2341 atgccgtctg aaaagtggaa tgcgctattt taatagaaac ggtttaaggt cgtctgaaaa
     2401 agtgcagcgg cagggcagca cttttccaat cgacggtttg atgatgcagc acagaagata
     2461 ttgacaaacc gccgcccctg catatagatt cattaaaaca taaccaaaat gaacaacaaa
     2521 tgagaataga gatcacacca atcagcgaat ccgctttggt ctgccgactg aatgcgcctt
     2581 ccgaactggg caaacagcaa aagttgtggg cgtttgccgc tgcgctcggg cagcacgaca
     2641 ggattgagga agtggtggtc ggcatgaaca atctgaccgt gtttacccgt ttcgataccg
     2701 atttggcgac gcttgccgat gaattgcaat atgtgtggga acacaccgcc gttacagacc
     2761 atcagggcaa actggtggaa attcccgtct gctacggcgg cgaatacggc ccggatttgg
     2821 cggaagtcgc tgctttccat cagacggtta tttccgaaat cgtccgccgc catacggcgc
     2881 aaacttatac cgtatttatg atgggcttcc agcccggttt cccttatctg ggcggcttgc
     2941 ccgaagcatt gcacacgccc cgccgtgccg tgccgagaac gtccgttcct gccggttcgg
     3001 tcggtatcgg cggcagtcag accggtgtgt atccgttcgc ttcgcccggc ggctggcaga
     3061 ttatcggcag aaccgaatta cccttgttcc gagccgattt gaatccgccg accctgctgg
     3121 cggcgggtga ccaagtccgc tttgttgcag aaaggattga gccatgattc acgtttcggc
     3181 agtgcaggca ccggcgcata ttcaggatac cggacgctac ggacaccggc gttacggcat
     3241 cggtcatgcc ggtgcgatgg acacggttgc tttggcggcg ggtaatattt tattgggcaa
     3301 cgacgaaggc acggccgcaa tcgaaatcgc tttgggcggg ataatgctgg tgtttgaacg
     3361 ggatacgccg ttttgtctca ccggtgccgt gtatcaggcg gaattggacg gcgaaccggt
     3421 ctattcgtat tggcgttata ccgcccgcaa agggcagacc ttgaaaatgg tgcgtgccgt
     3481 gcagggcatg tacggctatg tgtgcgtggc gggcggattt gatgtgccgg aagtgatggg
     3541 ttcgagaagt accgacctga aagccggttt cggcggccat cagggcagaa tgctgcaaaa
     3601 aggcgattat ctccccatcg gcaaaggtgc gcaggaattg tccaaagtcg gcattgcccc
     3661 gataccgttt accgataccg tccaccttgt tccttcgtcg gaatatgccg ctttcagtga
     3721 aaaagggcgt ctgaatctgg aacgggaaac gtggacgctg caaagcgata gcaaccgcat
     3781 gggctaccgc ttcgacggac agccgctgac cctgtcgcaa cctttggaaa tgctgtccca
     3841 cgctgttcag gcaggaaccg tgcaggtgcc gcccggcggc aaaccgatta tcctgctggc
     3901 cgatgcacaa accaccggcg gttatccgaa aatcgctacc gttgccgccg ccgatttggg
     3961 caggctggca caggtgcgct tcggcagcaa agtcaaattc aaaataatcg gcttgaaaga
     4021 agccaccgcc ctgcggcgca aaaaccaagc ctatctgaac caaatacgga gaatcaccca
     4081 tgaagcaggt tgatttaaac gccgatctcg ccgaaggctg cggcagcgac gaagccttgt
     4141 tgcagctgat tacttcggcc aacatcgcct gcgcccaaca cgccggcagc attgccgata
     4201 ttcgggcggc attggcgtat gcccaacaaa acggcgtgcg catcggagca caccccggct
     4261 atcccgatcg ggaaaacttc ggccgtaccg aaatgaatct gtccgaagcc gatttgcggg
     4321 cgtgtctgaa ttaccagttg ggcgcattgc aggccttgtg ccgtgatcag ggtttggaaa
     4381 tggcttatgt caaaccgcac ggcgcaatgt acaaccaagc ggcgaaaaac cgtgcgctgg
     4441 cggataccgt tgcccgaatt gtggcggatt tcgacccgaa attgaaattg atggcacttt
     4501 ccggcagcct gctcttggaa gccggaaaag ccgcaggctt gggtgtgatt tccgaagtat
     4561 tcgccgaccg ccgctatatg cccgacggta cgctggttcc ccgcagccgc cccgatgcgc
     4621 aggttgacag cgacgaagaa gccatcgccc aagtattgca gatggtgcgg gacgggcggg
     4681 tcaaagcagt gaacggcagc ctggttgccg tgcaagccga cagcatctgt ctgcacggag
     4741 acgggccgca cgccgtggtg tttgccgaaa aaatccggca ggaactactg gcggcaggca
     4801 tcaaagtttc tgcttgaaaa acagttcata ggccgtctga aaaccgcagg tttcgccaaa
     4861 acgagcaaag cgagtttctg cgtagctaaa accgcaggtt tcgcccaaac acagcaacaa
     4921 ccaattttat agaggataag cgattatgtc tgatcaaaaa aaccgcagaa atgccttaat
     4981 cggcgctgca ttcctgatgg cgacttccgc catcggcccg ggctttctga cccaaaccgc
     5041 caccttcacc caagcactgg cggcaagttt cggctttgtg attctgctct cgattctgct
     5101 cgacatcggg gcgcagctca atatttggcg gattgtcgcc gtttccgaaa aacaggcgca
     5161 ggatattgcc aatcaggtct tgcccggcgc aggctatttc ttggctgtgc tgattgtgat
     5221 gggcggtttg gcgttcaata ttggcaacgt cggcggcgca ggcttgggtc tgaacctgct
     5281 gaccggactg tcgccggaaa ccggtgccgt gatcagcggc gtgattgcca tcggtgtgtt
     5341 tctgtttaaa gaagcaggca aagtgatgga caaattcgcc caagtgatgg gtttcgtaat
     5401 gattgcgctg acggtttatg tggcatggca ggcgaatccg ccgctggcag atgccgccgt
     5461 gcataccttt atgccggaaa aactcgatgc aatggcgatt gttacactgg tgggcggcac
     5521 ggtcggcggc tacatcacct tcgccggtgc gcaccgtctg ctggacgcag gcatcaaagg
     5581 caaatcggcg ttgccggaag tgagccaaag ctcggtgcgg gcgatcctga ttgcctcgat
     5641 tatgcggatt gtattgtttt tggcggtttt gggcgtggtc agccaaggcg tacagctcaa
     5701 tcccgacaac cctgcttcca caccgtttga atatgcggcg ggatacatcg gcctgctgat
     5761 tttcggcgtg gtgatttggg cggcttcgat tacttcggtg attggtgcgg cttatacttc
     5821 ggtgtcgttc ttctccggtc tcagcccgtc tatcgaacgc aataaaaaca aatggattat
     5881 tgcctttatc gccgtgtcca ccgccgtatt ttccaccatc ggcaaaccgg cgcaggtgct
     5941 ggtgttcgta ggcgcattaa acggcctgat tttaccgatt tccctcggtc tgattctgat
     6001 tgccgcctac aaaaccaaaa ttgtcggcga ctacaaacac ccgctgtggc tcaccgtttc
     6061 cggcgtgatt gtggtcggtt tgatggcagt actcagcgcc atcaccatca gcaaatatat
     6121 cggcggcttg ttcggctgaa ccttgtagcg cagatgtttg attttgtata ttatccgaag
     6181 tttgccgcct gatatggaaa attcctgttt cagacgatgt atccggtctg aaacctattc
     6241 acaactatga aacgcttcac ctatactctt tccgacggct tgtgcatcga aatcgaactc
     6301 aaacgcagtg ccaagaaaaa tctgattctg cgccccgtca atatgcagac ggtcagcatc
     6361 aacgtcccac ccttttttca agaccacgcg ttagcaaact ggctggcggc aaacgaaacg
     6421 attttgcgga acacgcttgc caaaacgccc gtgcatcctg tttcccaccc aaacttaccc
     6481 gagtggattt ggtatcgggg aataaagacc aagctggata cccacagcca aagccatatc
     6541 cgtatcacgt cgtctgaaat cctgcttccc cgaaaagaaa ccgccgcaca aatcgaccac
     6601 ctgcgccgcc tgttgaacga acgcgcccgc gaatacctgc tgccccgcct tgaaaaacac
     6661 gcagccgaaa caggactgac gcccaccgcc acagacctga gcaacgccaa aaccttttgg
     6721 ggcgtatgcc gcccgcacac cggcatccgc ctcaactggc ggctgatcgg cgcgcccaag
     6781 tacgttgccg actatgtctg catccacgaa ctctgccacc tccgccaccc cgaccacagt
     6841 ccgcgctttt ggcatttggt gaacacgctg acgccgcata ccgacaatgc taaaagttgg
     6901 ctgaaggcgc acgggcggga attgtttgtg ctggggtaaa ggctaaccgt agcgtgggct
     6961 tcgcccgcga gaatccaccc cctcccgtac ggagggagag ggttggagag agggtggctc
     7021 ttgtcccttg ttctgtatat ttgcaagcca ccctatccta gccttccccc gttggataag
     7081 ggaaagggat caagctgctg taactgaaa
//
LOCUS       DAXIHS010000091         7069 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXIHS010000091 DAXIHS010000000
VERSION     DAXIHS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.091"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(246..1592)
                     /gene="mnmE"
                     /locus_tag="ACFOF2_001770"
     CDS             complement(246..1592)
                     /gene="mnmE"
                     /locus_tag="ACFOF2_001770"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC7108037.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1723..1878
                     /locus_tag="ACFOF2_001771"
     CDS             1723..1878
                     /locus_tag="ACFOF2_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108038.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1875..2306
                     /locus_tag="ACFOF2_001772"
     CDS             1875..2306
                     /locus_tag="ACFOF2_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC7108039.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGS
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2440..6810
                     /gene="app"
                     /locus_tag="ACFOF2_001773"
     CDS             2440..6810
                     /gene="app"
                     /locus_tag="ACFOF2_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC7108040.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETQPATTAFP
                     RARRARRDLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAE
                     DRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTF
                     DDGIGNSARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQA
                     RYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQ
                     HISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAA
                     AKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1890 a         2026 c         1710 g         1443 t
ORIGIN      
        1 ttcaaaaaaa ctatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa
       61 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact
      121 gtctgcggct tcgttgcctt gtcctgattt ttgttaatcc actataaaac caaacggatc
      181 ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg atgctttcga
      241 tccgtttatt ttccgataca aaacctcgaa aaaatcacgc cgagcaggtc gtccgccgta
      301 aactcgcccg tgatttcgcc gcatgcgacc tgcgccaagc gcaagtgttc ggcaaacagc
      361 tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc tgctttgagt
      421 gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc cctcgccctg ccatccggcc
      481 tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc cgacagcgcg
      541 atgacggttt ccgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa atcggatttg
      601 ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc ccgtgtcttg
      661 tcgttcacgc cttcgcgcgg atcgaccaac accagcgcga catcggcttc ggatacggct
      721 ttgcggctgc gttcgatgcc gatgcgttcg accacgtcgt ccgtctcgcg caaacctgcc
      781 gtatcgacaa tatgcaccgg aacgccgtca atcaggatac gttccctgac cgcgtcgcgc
      841 gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccagcgc gttcaacagg
      901 ctggatttgc cgacattcgg cgcgccgacc aatacgacat tcagaccttc gcgcaaaatc
      961 gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc gtccagtttg
     1021 ccgcgtgcgt ctgccgcttc gagaaaatca atgtcttcct cgggaaaatc taacgtcgct
     1081 tcgaccagca tccgcaaggt aatcaagtct tcgaccagac cgtgtatccg ccgcgaaaaa
     1141 tcgcctttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc gtcaatcaaa
     1201 tccgccacgc cttccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc acgcttcgta
     1261 aactcgcccg gttcggcaag gcgcgcgccc aattccaaac agcggttcag cagcatatcc
     1321 atcactaccg gcccgccgtg tccctgaagc tcgataacat cttcgcccgt aaaacttgcc
     1381 ggtgcggcaa aaaaaagcaa aagcccgctg tcgattgcct gaccgtccgc gtccgtaaaa
     1441 tcagcatagg ttgccacgcg cggcttgggc gttttcccac acaaagcctc tgccatcggc
     1501 agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgcccggg tgcggtagcg
     1561 actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca aaacagccgc
     1621 gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca taaaaccgca
     1681 aaaatgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa acgaaacaca
     1741 tacgccttcc tgctcggtat aggctcgctg ctgggtctgt tccatcccgc aaaaaccgcc
     1801 atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca acgcgccata
     1861 gagaaagcgc gcaaatgacc gaaaacgcac aggacaaggc gcggcaggct gtcgaaaccg
     1921 tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg caaataatga
     1981 tagcccggcg tttccattcg ggatcgttgc cgccgccgtc cgacttggcg caatacaacg
     2041 acattatcag caacggggca gaccgcatta tggcaatggc ggaaaaagaa caagccgtcc
     2101 ggcacgaaac catacggcaa gaccaaacct tcaacaggcg cgggcaactg tacggcttca
     2161 tcagcgtcat cctgatactg ctttttgccg tcttcctcgt atggagcggc taccccgcaa
     2221 ccgccgcctc ccttgccggc ggcacagtgg ttgccttggc gggtgctttc gtgattggaa
     2281 gaagccgaga ccaaggcaaa aattaatgac aaatcctagg gcgtgcttca tatccgcccg
     2341 aacgccgaac cgcacatata ggcacatccc gcgcgccgcc cggaagcgga agccgcgccc
     2401 tcccaaacaa acccgaatcc cgtcagataa ggaaaaataa tgaaaacaac cgacaaacgg
     2461 acaaccgaaa cacaccgcaa agccccgaaa accggccgca tccgcttctc gcctgcttac
     2521 ttagccatat gcctgtcgtt cggcattctt ccccaagctt gggcgggaca cacttatttc
     2581 ggcatcaact accaatacta tcgcgacttt gccgaaaata aaggcaagtt tgcagtcggg
     2641 gcgaaagata ttgaggttta caacaaaaaa ggggagttgg tcggcaaatc aatgacaaaa
     2701 gccccgatga ttgatttttc tgtggtgtcg cgtaacggcg tggcggcatt ggtgggcgat
     2761 caatatattg tgagcgtggc acataacggc ggctataaca acgttgattt tggtgcggaa
     2821 ggaagcaatc ccgatcaaca tcgttttact tataaaattg tgaaacggaa taattataaa
     2881 gcagggacta acggccatcc ttatggcggc gattatcata tgccgcgttt acataaattt
     2941 gtaaccgatg cagaacctgt tgaaatgacc agttatatgg atgggcggaa atatatcgat
     3001 caaaataatt accctgaccg tgttcgtatt ggggcaggca ggcaatattg gcgatctgat
     3061 gaagatgagc ccaataaccg cgaaagttca tatcatattg caagtgcgta ttcttggctc
     3121 gttggtggca atacctttgc acaaaatgga tcaggtggtg gcacagtcaa cttaggtagt
     3181 gaaaaaatta aacatagccc atatggtttt ttaccaacag gaggctcatt tggcgacagt
     3241 ggctcaccaa tgtttatcta tgatgcccaa aagcaaaagt ggttaattaa tggggtattg
     3301 caaacgggca acccctatat aggaaaaagc aatggcttcc agctggttcg taaagattgg
     3361 ttctatgatg aaatctttgc tggagatacc cattcagtat tctacgaacc acgtcaaaat
     3421 gggaaatact cttttcacga caataataat ggcacaggaa aaatcgacgc caaacatgaa
     3481 cacaattctc tgcctaatag attaaaaaca cgaaccgttc aattgtttaa tgtttcttta
     3541 tccgagacag cacaagaacc tgtttatcat gctgcaggtg gtgtcaacag ttatcgaccc
     3601 agactgaata atggagaaaa tatttccttt attgacgaag gaaaaggcga attgatactt
     3661 accagtaaca tcaaccaagg tgctggagga ttatatttcc aaggagattt tacggtctcg
     3721 cctgaaaaca acgaaacgtg gcaaggcgcg ggcgttcata tcagtgaaga cagtaccgtt
     3781 acttggaaag tcaatggcgt ggcaaacgac cgcctgtcca aaatcggcaa aggcacgctg
     3841 cacgttcaag ccaaagggga aaaccaaggc tcgatcagcg tgggcgacgg taaagttatt
     3901 ttagatcaac aagcagatga aaataataaa aaacaagcct ttagtgaaat cggcttggtc
     3961 agcggcaggg gtacggtgca actgaatgcc gataatcagt tcaaccccga caaactctat
     4021 ttcggctttc gcggcggacg tttggatttg aacgggcatt cactttcgtt ccaccgtatt
     4081 caaaataccg atgaaggggc gatgattgtc aaccacaatc aagacaaaga atccaccgtt
     4141 accattacag gcaataaaga tattgctaca accggcaata acaacagctt ggatagcaaa
     4201 aaagaaattg cctacaacgg ttggtttggc gagaaagata cgaccaaaac gaacgggcgg
     4261 ctcaaccttg tttaccagcc cgctgcagaa gaccgcaccc tgctgctttc cggcggaaca
     4321 aatttaaacg gcaacatcac gcaaacaaac ggcaaactgt ttttcagcgg cagaccaaca
     4381 ccgcacgcct acaatcattt aaacgaccat tggtcgcaaa aagagggcat tcctcgcggg
     4441 gaaatcgtgt gggacaacga ctggatcaac cgcacattta aagcggaaaa cttccaaatt
     4501 aaaggcggac aggcggtggt ttcccgcaat gttgccaaag tgaaaggcga ttggcatttg
     4561 agcaatcacg cccaagcagt ttttggtgtc gcaccgcatc aaagccacac aatctgtaca
     4621 cgttcggact ggacgggtct gacaaattgt gtcgaaaaaa ccattaccga cgataaagtg
     4681 attgcttcat tgaccaatac cgacatcagc ggcaatgtca gccttgccga tcacgctcat
     4741 ttaaatctca cagggcttgc cacactcaac ggcaatctta gtgcaaatgg cgatacacgt
     4801 tatacagtca gccacaacgc cacccaaaac ggcgacctta gcctcgtggg caatgcccaa
     4861 gcaacattta atcaagccac attaaacggc aacacatcgg cttcgggcaa tgcttcattt
     4921 aatctaagcg accacgccgt acaaaacggc agtctgacgc tttccggcaa cgctaaggca
     4981 aacgtaagcc attccgcact caacggtaat gtctccctag ccgataaggc agtattccat
     5041 tttgaaagca gccgctttac cggacaaatc agcggcagca aggatacggc attacactta
     5101 aaagacagcg aatggacgct gccgtcaggc acggaattag gcaatttaaa ccttgacaac
     5161 gccaccatta cactcaattc cgcctatcgc cacgatgcgg caggggcgca aaccggcagt
     5221 gcgacagatg cgccgcgccg ccgttcgcgc cgttccctat tatccgttac acctccggct
     5281 tcggcagaat cccatttcaa cacgctgacg gtaaacggca aattgaacgg tcagggaaca
     5341 ttccgcttta tgtcggaact cttcggctac cgcagcgaca aattgaagct ggcggaaagt
     5401 tccgaaggca cttacacctt ggctgtcaac aataccggca acgaacctgc aagcctcgaa
     5461 caattgacgg tagtggaagg gaaagacaac aaaccgctgt ccgaaaacct taatttcacc
     5521 ctgcaaaacg aacacgtcga tgccggcgcg tggcgttacc aactcatccg caaagacggc
     5581 gagttccgcc tgcataatcc ggtcaaagaa caagagcttt ccgacaaact cggcaaggca
     5641 gaagccaaaa aacaggcggg aaaagacaac gcgcaaagcc ttgacgcgct gattgcggcc
     5701 gggcgcgatg ccgtcgaaaa gacagaaagc gttgccgaac cggcccggca ggcaggcggg
     5761 gaaaatgtcg gcattatgca ggcggaggaa gagaaaaaac gggtgcaggc ggataaagac
     5821 agcgccttgg cgaaacagcg cgaagcggaa acccagccgg ctaccaccgc cttcccccgc
     5881 gcccgccgcg cccgccggga tttgccgcaa ccgcagcccc aaccgcaacc tcaaccccaa
     5941 ccgcagcgcg acctgatcag ccgttatgcc aatagcggtt tgagtgaatt ttccgccacg
     6001 ctcaacagcg ttttcgccgt acaggacgaa ttggaccgcg tgtttgccga agaccgccgc
     6061 aacgccgttt ggacaagcgg catccgggac accaaacact accgttcgca agatttccgc
     6121 gcctaccgcc aacaaaccga ccttcgccaa atcggtatgc agaaaaacct cggcagcggg
     6181 cgcgtcggca tcctgttttc gcacaaccgg accgaaaaca ccttcgacga cggcatcggc
     6241 aactcggcac ggcttgccca cggcgccgtt ttcgggcaat acggcatcgg caggttcgac
     6301 atcggcatca gcacgggcgc gggttttagc agcggcagtc tttcagacgg catcggaagc
     6361 aaaatccgcc gccgcgtgct gcattacggc attcaggcac gataccgcgc cggtttcggc
     6421 ggattcggca tcgaaccgca catcggcgca acgcgctatt tcgtccaaaa agcggattac
     6481 cgctacgaaa acgtcaatat cgccaccccc ggccttgcgt tcaaccgcta ccgcgcgggc
     6541 attaaggcag attattcatt caaaccggcg caacacattt ccatcacgcc ttatttgagc
     6601 ctgtcctata ccgatgccgc ttcgggcaaa gtccgaacgc gcgtcaatac cgccgtattg
     6661 gctcaggatt tcggcaaaac ccgcagtgcg gaatggggcg taaacgccga aatcaaaggt
     6721 ttcacgctgt ccctccacgc tgccgccgcc aaaggcccgc aactggaagc gcaacacagc
     6781 gcgggcatca aattaggcta ccgctggtaa ccgccggata tgccgaaagg ggtctgacga
     6841 tgccaccgtg cgctgtcaaa ccctttttct gccgccgcct tgtgcctgct ccattgtctg
     6901 ataaccgtca aattgatttt tagcccatgt ttggtgggtc ggggaaatct atatcttcgt
     6961 ccgtgccgaa atagtctgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
     7021 aaacacaggt tttcgtctat tttcgcccca aatacctcct aattctacc
//
LOCUS       DAXIHS010000092         6929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXIHS010000092 DAXIHS010000000
VERSION     DAXIHS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.092"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..266
                     /locus_tag="ACFOF2_001774"
     CDS             <1..266
                     /locus_tag="ACFOF2_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC7108041.1"
                     /translation="CPIKPKPTNHAQQRKEEAKNDSHRSVGDSNRVVREGKQYLDSDT
                     GNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKNGKWTPK"
     gene            244..525
                     /locus_tag="ACFOF2_001775"
     CDS             244..525
                     /locus_tag="ACFOF2_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108042.1"
                     /translation="MGNGHQNNRSPLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            904..2037
                     /locus_tag="ACFOF2_001776"
     CDS             904..2037
                     /locus_tag="ACFOF2_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108043.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NTNAFVAKLARLFRNADRAVVIVKESMRTEESLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGYGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMTRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLDKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(2124..2399)
                     /locus_tag="ACFOF2_001777"
     CDS             complement(2124..2399)
                     /locus_tag="ACFOF2_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108044.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKARLMCISVFWIVVFNL"
     gene            complement(2416..3189)
                     /locus_tag="ACFOF2_001778"
     CDS             complement(2416..3189)
                     /locus_tag="ACFOF2_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC7108045.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFT
                     VQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3575..3850)
                     /locus_tag="ACFOF2_001779"
     CDS             complement(3575..3850)
                     /locus_tag="ACFOF2_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC7108046.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(3847..5130)
                     /locus_tag="ACFOF2_001780"
     CDS             complement(3847..5130)
                     /locus_tag="ACFOF2_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC7108047.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="ACFOF2_001781"
     CDS             complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="ACFOF2_001781"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC7108048.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
BASE COUNT         1872 a         1640 c         1674 g         1743 t
ORIGIN      
        1 cgtgtccgat caaaccaaaa ccaaccaatc atgcccaaca aagaaaagaa gaagctaaaa
       61 acgattctca tcgaagtgtg ggagattcca atcgtgtcgt tcgcgaagga aagcaatatt
      121 tagattccga cacaggaaac catgtttatg taaaaggaga taaagtggtt attctaactc
      181 ctgatggaag acaggtaact caatttaaga actcgaaagc caatacgtca aaaagggtaa
      241 aaaatgggaa atggacacca aaataatcga tctcccttag aaaaaagaat tttttatctg
      301 gagcattcgg ggcagtatct gatgatttgc gccctatcag attactccca aaataaacat
      361 actgtcgtta tggcgaattt tctctatcca aatgaaaaaa tggattggag aaatttagat
      421 gatttgttca atgagttggt tttagaggaa ttacagtctt catttatgga ttggtatccg
      481 actattgaag aggcaattag ccatcattta gaagactttt cgtaacaagc ataaattaaa
      541 ggttgtctga aactatgttc gattttcgac gacattttcc tcattggagg gggcggcaat
      601 ttatggaaaa tagatttgaa aaaatcgtta aggatatttt tagttttacc caatttgttc
      661 cgaagcagaa aatgagattc ccaccccatc ctcccgttat gacgactgtt ggtgggaatt
      721 ggaaatcttg aatgcctctg catttgaatt agtcaaaaaa ttgaatcaaa gcaaacaaga
      781 gtaacaagct gcagcctgca taggggtttt taaagtacgg cagcaaccgt agattaaatt
      841 aaaaatcaaa caatcaaagc tcgtctgata taatcgatat attctgttaa acgaaaaata
      901 aatatgttta aaaaattcaa accggtactg ttgtcatttt ttgcacttgt atttgccttt
      961 tggctgggga cgggtattgc ctatgagatt aatccgcgtt ggtttttgag cgatacggca
     1021 actgaaatac ctgaaaatac gaatgctttt gtggcgaaac ttgcccgcct gttccgaaat
     1081 gccgacaggg cggttgtcat cgtgaaggaa tcgatgagga cggaggaaag tcttgccgga
     1141 actgtggatg acggtccgtt gcagtcggag aaggattatc tcgcgctcgc tatccggctc
     1201 agtcgtttga aagaaaaggc gaaatggttt cacgtaacgg agcaggaaca tggggaagag
     1261 gtttggctgg attactatat cggcgagggc ggtttggttg cggtttcgct ttcgcaacgc
     1321 tcgccggaag cgtttgttaa tgccgaatat ctgtatcgga acgatcgtcc gttttctgta
     1381 aatgtgtacg gcggaacggc tcacggagaa aattatgaaa cgacaggaga atatcgggtt
     1441 gtttggcaac cggacggttc ggtatttgat gcgtcgggat acgggaaaat cggggaagat
     1501 gtttatgagc attgcctcgg gtgttatcag atggcccagg tatatttggc gaaataccgg
     1561 gatgtcgcga atgatgagca gaaggtttgg gacttccgcg aagagagcaa ccggattgcg
     1621 tcggactcgc gcgattctgt gttttatcag aatatgcggg aattgatgac ccgggggatg
     1681 aaggcgaaca gtcttgtggt cggctatgat gcggacggtc tgccgcaaaa agtctattgg
     1741 agtttcgaca atgggaaaaa acgccagagt ttcgaatatt atttgaaaaa cggaaatctt
     1801 tttattgcac aatcttcgac ggtagcattg aaagcggatg gcgtaacggc ggatatacag
     1861 acctatcatg cgcaacagac gtggtatttg gatggcgggc ggattgtccg cgaagagaaa
     1921 cagggagaca gactgcctga ttttcctttg aacttggaag atttggacaa agaagtgcgc
     1981 cgttatgcgg aggctgcggc gagacgttcg ggcggcaggc gcgacctttc tcactgaacg
     2041 caaatgccgt ctgaaaccgg ttttcagacg gcattggaga gtcaaacgct ttttgcaata
     2101 ccgtatatgc cgaatccgat tcgctacaaa ttgaaaacca caatccaaaa aacggaaata
     2161 cacattaaac gggccttttt accgaccgta tcatcccata ggttttccac accttttttc
     2221 atccataaat tcaaaccatt taagtttgta tccttaccga caattgcagc agcatcatcc
     2281 gcataacggt cacccaaatt agccaaataa cgccaaccgc cataggtatc gccacggaaa
     2341 gcataaccac gcaaggtttg gatttgttct ggtgttaaaa caacttcacc ctcattcata
     2401 ataagatcct ttaaattatt ttttacttga attgtttaca aaactctgta ccaattgctc
     2461 tacttgttgg tgataaccta ccaactcctg ctgtgtgagc tgctgctttt cccgaataaa
     2521 cgggtaatac tgtaccgtga aaccgttaat acaggaacct acgctagtgc tataattatc
     2581 ttcttcagcc tggtttactc gattattcca atgagtaata atcgccggat tttctccttt
     2641 tttaatatag taaaattcat gaaaatatgg attttcagga atatgtcttt tatcttcata
     2701 agcttcatat tcaggatagg cagatggcat ttttttaacc ccgtaagtaa gcaatctttc
     2761 tcccatagtc gtacttaaac tacacgcatc ttttcggata acaatatcgt ccacgctatc
     2821 caaaccatcg cgtagaaatt ggataaagtg tgctttgttt tgctggttgt atggctcaag
     2881 aacccagcct tggtcggttt ttttgtaata gacgctaatc tctatcaaat taatggagta
     2941 agcatcctgc ccgggcgtgt cggaaagcca ttttgtggca ttgtcttgcg tataccgtgc
     3001 aagatagcca taagggataa ccattttggt gccctgaaaa tcaaatgtaa ccgtatgttc
     3061 aaatcctgtc attggctcgg gattgttgaa actggtttgt tcattaacag gttcacatga
     3121 ggtcatagta aaaacactcc caataaacaa aataaaaagt tgataaatgg tagtagcata
     3181 tggtctcata atttcaagcc tagaaattag ttaaagaata tattatggta gatactaact
     3241 ctttaagttg acatattgtg cgatgtcgag ggctgtacca tattttccca tcgtattttc
     3301 ccgttcttta tatgagcggc aggcatcctc ataaaagaag ggataccgat acggaaaaat
     3361 accccggcat aaaaaatata ctgattaatc aatacaaatc caacaaacag ttcatcgtat
     3421 tcttttctgt tggcgtataa aaaatactga tatatgcctt cactttcata gggcgacgct
     3481 ttatgtaccg tcctgtgtgt tgaaacgtcg gttttcctta ccgcctgccc cgcttttccc
     3541 tgattgctgc caactgccgt ccgaacctga accgtcaggt ttcagacggc attttttccg
     3601 cctgttccca caatttccgt ttttgtgcgg cggtcagttt tttgacggcg atttcctgtt
     3661 tgagcgccgt gcctttatcc atcccgcctg caacgatacg catcgccacc ggtttgaata
     3721 cgcgggtata tttcgcgcct ttaccggttg tgtgggcggc aagccgctgt tgcggattcg
     3781 ggctgatgcc gcaatagaac gcgctgtttt cacacaatat caggtaaacg ctccagtttg
     3841 acgcgttcat ctgcctgcat cctgagtttt ttccccgttc caaggcgcaa ctttcagcag
     3901 cagcagcata cccggcaggg caagtatgaa acacagctgg aaaaacggta cataaccgag
     3961 ccattcgatc agataaccgg caaaggaatt gatgaccgtg cgcgggacgg cggacaggct
     4021 ggtaaacagc gcaagctgcg ttgcggtaaa tgcgggattg gtttcacgcg ccatatacga
     4081 tacgaacgcc gccgtcccca accccacgcc gaccgcttcc gcgccgataa ctgccgccag
     4141 catcagcctc tcgcctgtgc cgaccgtgtc gaaaggtccg aaccctgcca gccatacaaa
     4201 ccccaaaacg gttacagcct gcaccgcgcc gaatagccac aaggctttgt ttacgccgat
     4261 tttcagcatc cacacgccgc ccaagatacc tgccgccact gccggccaca gtcctgcatt
     4321 ttttgcaatc aaaccgatgt ctgtcttact gaaacccata tccagataaa acggcgttgc
     4381 caacgcggtt gccatactgt cgccgagttt gtaaaggaag ataaacagca gcacgcacac
     4441 cgccgaagcg atgcccttgc gcgtaaaaaa ttctttaaac ggctctacca cggtctgctt
     4501 caacgtttta ggaacggcag gcggcaacac gggttcgcgc gcaagaaaca gcgtcatcag
     4561 aagaccgggc agcataaaca gcgaagtgat gacaaacacc tccggccacg gcatcctgtc
     4621 tgccagcacc aaactcaatg aaccgggaat cagggcggca acccgatagg cgttcacatg
     4681 aaccgagttg cccaaaccca attcctcatc cgacaaaatc tcgcgcctga acgcatccaa
     4741 tacaatgtcc tgactggcgg aaaaaaaagc gacaagcacc gacaagccgg caatcagcgg
     4801 cagatgatta cgagggttta aaaaggcata agccgccaaa gccgccagca aacctgcctg
     4861 cgtcagcagc atccacccgc gccgccgccc caaaacgggc agcctgaccg cgtccatcag
     4921 cggcgaccac aaaaatttcc aagtaaacgg cagaccgatt aacgccatca gcccgatgct
     4981 cttcaaatcc acctgctcgc tgcgtaacca cgccggaatc aggttaatca gaaagtacag
     5041 cggcagcccc gaggcaaacc ccgtaaaaat acagacaagc atattgcggg aaaagatttg
     5101 cccgataaaa cctgatttcg atgcagtcat cgttcgtgca gcccaaaaaa aacaaaagcc
     5161 tattgtagca aatatcgccc gcctgctgaa aaacgccgcc ccgcgccgcc ggtttgccgt
     5221 tcaaatgcaa aatgccgtct gaaactgtgt tcagacggca ttgcttttct gaaaaccaga
     5281 cgtgcttaca ggctgtaata catttcaaat tccagcgggt gcggcgccat gcggatgcgg
     5341 cgtacgtctt cttctttgaa agcgatgtag ctgtcgatcc agtctttgct gaacacgccg
     5401 ccgcgcagga ggaattcgtg gtcggctttg agggcggcca gtgcttcttc caaagaagcg
     5461 caaacggtcg gcaccaatgc atcttcttcc ggcggcagat cgtacaggtt tttatcggca
     5521 gggtcgcccg gatggatttt gttttgaatg ccgtccaaac ccgccatcaa cagggcggca
     5581 aatgccaaat acgggttggc ggtcggatcg gggaaacgcg cttcgatgcg gcgcgccttg
     5641 ctgctgttca cggacggaat gcggatggaa gcggaacggt ttttggcgga gtatgccagt
     5701 ttggtcggcg cttcaaagtg cggcacgagg cgtttgtagg agttggtgga cggattggta
     5761 atcgcgttca aggctttggc gtgtttgatg atgccgccga tgtagtagag cgcggtgtcg
     5821 ctcaagccgg catagccgtc gcctgcgaac aggttttgac cgtctttcca aatggattgg
     5881 tgaacgtgca taccgctgcc gttgtcgccc ataatgggtt tgggcatgaa agtggcggtt
     5941 ttgccgaagt tgtgggcaac gttttgaatc acatatttca tgtcttgggt ttggtcggcg
     6001 cgtttgacca aagtagcaaa gcgcgtgccg atttccattt ggctgccggt gccgacttcg
     6061 ctgtggtgca cttccacttc aataccgagt tcttccaaaa tgtttaccat cgccgaacgc
     6121 aaatcctgac cgcagtcaat cggtgcaaca ggtgcgtaac cgcctttgac ggtcgggcgg
     6181 tggccggtgt tttgaccgtc catatgcaga ccgcttgccc acgcgccgct ttcggacgtg
     6241 atttcgtaac gggttttgtg catatcggtt tcaaattcta tgccgtcgaa tacgaaaaac
     6301 tcgggttcgg gaccgaaata ggcggtctcg ccgataccgg aagatttcaa ataggcttcg
     6361 gctcggcggg cgatggagcg cgggtcgcgg tcgtaaccct gaccgtcggc gggatcgata
     6421 acgtcgcaag tcaacacaac agtcgcatca tcataaaaag gatcgacgaa ggctgtagac
     6481 gcatcgggac gcaactgcat atcggaagcc tgaatgcctt tccagccgcc gatagacgaa
     6541 ccgtcaaacg cctgaccgtt ttcgaaccac tcttcggggt cttccaacac gatgcgcgca
     6601 ggcacggtaa agtggtgctg cttgcctttg gtatcggtaa agcgcaaatc gacaaagcgg
     6661 gcttcgcttt cttcaatcaa ttttacggcg tttttaatag acatttttca gctcctggaa
     6721 aagggaaata gcaaaatata cggaacgcgg cagattagcc caaagccagc atgattctac
     6781 cacgtccatg ccgtctgaaa ctcgccctgc gtcaaatttc aagctggaaa atatagtttc
     6841 agacggcaag attgtcaaca atatgcttcg gtttgtctgt tcaaaagcag aatacccgcc
     6901 cttttgtccg aatgccgaca aaacagatg
//
LOCUS       DAXIHS010000093         6621 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXIHS010000093 DAXIHS010000000
VERSION     DAXIHS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.093"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..1739
                     /locus_tag="ACFOF2_001782"
     CDS             <1..1739
                     /locus_tag="ACFOF2_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC7108049.1"
                     /translation="FFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAELLT
                     DANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTGMC
                     LGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRD
                     GKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKSDL
                     QIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTD
                     LTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQS
                     SLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNR
                     WQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQFSY
                     ETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRHQF
                     YLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQTA
                     NTVYGFNLNYSF"
     gene            1832..>6621
                     /gene="hrpA"
                     /locus_tag="ACFOF2_001783"
     CDS             1832..>6621
                     /gene="hrpA"
                     /locus_tag="ACFOF2_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC7108050.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTGAGAAIIALAVTVVTSGAG
                     TGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAA
                     TAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAA
                     LVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDG"
BASE COUNT         1938 a         1708 c         1584 g         1391 t
ORIGIN      
        1 aattttttcc tgctccatgt ctgttggtta tcctggctgt catacccctt aaaaccttag
       61 ctgccgatga aaacgatgca gaacttatcc gttccatgca gcgtcagcag cacatagatg
      121 ctgaattgtt aactgatgca aatgtccgtt tcgagcaacc attggagaag aacaattatg
      181 tcctgagtga agatgaaaca ccgtgtactc gggtaaatta cattagttta gatgataaga
      241 cggcgcgcaa attttctttt cttccttctg tgctcatgaa agaaacagct tttaaaactg
      301 ggatgtgttt aggttccaat aatttgagca ggctacaaaa agccgcgcaa cagatactga
      361 ttgtgcgtgg ctacctcact tcccaagcta ttatccaacc acagaatatg gattcgggaa
      421 ttctgaaatt acgggtatca gcaggcgaaa taggggatat ccgctatgaa gaaaaacggg
      481 atgggaagtc tgccgagggc agtattagtg cattcaataa caaatttccc ttatatagga
      541 acaaaattct caatcttcgc gatgtagagc agggcttgga aaacctgcgt cgtttgtcga
      601 gtgttaaaac agatattcag attataccgt ccgaagaaga aggcaaaagc gatttacaga
      661 tcaaatggca gcagaataaa cccatacggt tcagtatcgg tatagatgat gcgggcggca
      721 aaacgaccgg caaatatcaa ggaaatgtcg ctttatcgtt cgataaccct ttgggcttaa
      781 gcgatttgtt ttatgtttca tatggacgcg gtttggcgca caaaacggac ttgactgatg
      841 ccaccggtac ggaaactgca agcggatcca gaagttacag cgtgcattat tcggtgcccg
      901 taaaaaaatg gctgttttct tttaatcaca atggacatcg ttaccacgaa gcaaccgaag
      961 gctattccgt caattacgat tacaacggca aacaatatca gagcagcctg gccgccgagc
     1021 gcatgctttg gcgtaacagg tttcataaaa cttcagtcgg aatgaaatta tggacacgcc
     1081 aaacctataa atacatcgac gatgccgaaa tcgaagtgca acgccgccgc tctgcaggct
     1141 gggaagccga attgcgccac cgtgcttacc tcaaccgctg gcaactggac ggcaagttat
     1201 cttacaaacg gggtaccggt atgcgccaaa gtatgcccgc acctgaagaa aacggcggcg
     1261 gtactattcc aggcacatcc cgtatgaaaa tcataaccgc cggattggat gcagcggccc
     1321 cgtttatgtt gggcaaacag cagttttcct acgaaaccgc tatccatgca caatggaaca
     1381 aaacgccttt ggttgcccaa gacaagttgt ctatcggcag ccgctacacc gttcgaggat
     1441 ttgacggtga acagagcctt tccggagagc gtggtttcta ctggcagaac actttgggct
     1501 ggcatttcca tccgcgccat caattctatc tcggcgcgga ctatggtcgg gtatcgggcg
     1561 aaagtgccca atatgcctcg ggcaagcggc tggcgggtgc agtggccggc ttcagaggca
     1621 gccataaagt aggcggtata tttgcttatg acttatttgc cggcaagccg ctgcacaagc
     1681 ccgaaggttt tcagacggcc aacaccgttt acggcttcaa tttgaattac agtttctaac
     1741 cctctcctgc attttttacc gacattcaga cagtctttca tcatttacag gccgtctgaa
     1801 gcgattacaa acatcattta aagggataaa tatgaacaaa ggtctgcatc gtaccgtttt
     1861 cagcaaaaag cacaacacta tggttgcagt tgccgaaacc gccaacagtc agggaaaagg
     1921 caaacaggca ggaacttccg ctccggcttc gccacaaatt tcagacggcc tttacagtaa
     1981 actcaaaacc acgcttaaaa ccttggtctg ctctttggtt tccgtcagta tggtattgcc
     2041 tgcccacgca caaatcatta ccgataaaac cgcgcccaaa aaccagcagg ccgttattct
     2101 taaaaccaat accggtgcgc cgttggtcaa catccaaact cctaatgccc gtggattgag
     2161 ccacaaccgc tatacccagt ttgatgtcga cgctaaaggt gctgtactga acaacgatcg
     2221 caacaacaac ccgtttctgg ccaaaggcag tgcgcagttg attttgaacg aggtgcgcgg
     2281 tgcggccagc aaactcaatg gcatcgttac cgtaggtggt caaaaggccg atgtgattat
     2341 cgccaacccc aacggcatta ccgtgaacgg cggcggcttt aaaaacgtcg gccgcggtat
     2401 cttaaccacc ggtacgcctc aaatcggcaa agacggtgcc ttgacgggat ttgacgtgcg
     2461 ccaaggcaca ttgaccgtcg gtacgtcagg ttggaacgac aaaggcggag ccgattacac
     2521 cgaagtgctt gcccgtgcgg ttgctttgca ggggaaattg cagggtaaaa acctggcggt
     2581 ttcgaccggc gcacagaaag tagattacgc cagcggtgaa atcagcgcag gtacggcagc
     2641 gggtacgaaa ccgaccgttg ccctcgatac tgccgcattg ggtggtatgt acgccgacag
     2701 cattaccctg attgccaatg aaaaaggcgt gggtgtcaaa aatgcgggca cactcgaagc
     2761 ggccaagcaa ttgatcgtta cttcctccgg ccgtattgaa aacagcggcc gcatcgccac
     2821 cactgccgac agcactgaag catcaccgac ttatctctcc atcgaaacca ccgaaacagg
     2881 cgcagcaggc gcatttatct ccaatggcgg ccggatagag agcaaaggct tattggttat
     2941 tgataccggg gaggacatcg gtttgcgtaa cggagcggtc gttcaaaaca acggcaaccg
     3001 ccctgctgct acggtattaa atgccggccg taatttggtt atcgaaagca aagcaaatgt
     3061 gaacaatgct aaaggttcga ccaatctgtc gaccggcggc cgtaccgtca tcaaggaggc
     3121 cagtattcag actggcacta ccgtatacag ttccagcaaa ggcaacgccg aattaggcaa
     3181 taacacacgc attaccgggg cagatgttac cgtattatcc aacggcacca tcagcagttc
     3241 cgccgtaata gatgccaaag acaccgcaca catcgaagca ggcaaaccgc tttctttgga
     3301 agcttcaaca gttacctccg atatccgctt aaacggaggc agtatcaagg gcggcaagca
     3361 gcttgcttta ctggcagacg ataacattac tgccaaaact accaatctga atactcccgg
     3421 caatctgtat gttcatacag gtaaagatct gaatttgaat gttgataaag atttgtctgc
     3481 cgccagcatc catttgaaat cggataacgc tgcccatatt accggcacca gtaaaaccct
     3541 cactgcctca aaagacatgg gtgtggaggc aggctcgctg aatgttacca ataccaatct
     3601 gcgtaccaac tcgggtaatc tgcacattca ggcagccaaa ggcaatattc agcttcgcaa
     3661 taccaagctg aacgcagcca aggctctcga aaccaccgca ttgcagggca atatcgtttc
     3721 agacggcctt catgctgttt ctgcagacgg tcatgtatcc ttattggcca acggtaatgc
     3781 cgactttacc ggtcacaata ccctgacagc caaggccgat gtcaatgcag gatcggttgg
     3841 taaaggccgt ctgaaagcag acaataccaa tatcacttca tcttcaggag atattacgtt
     3901 ggttgccggc aacggtattc agcttggtga cggaaaacaa cgcaattcaa tcaacggaaa
     3961 acacatcagc atcaaaaaca acggtggtaa tgccgactta aaaaacctta acgtccatgc
     4021 caaaagcggg gcattgaaca ttcattccga ccgggcattg agcatagaaa ataccaagct
     4081 ggagtctacc cataatacgc atcttaatgc acaacacgag cgggtaacgc tcaaccaagt
     4141 agatgcctac gcacaccgtc atctaagcat taccggcagc cagatttggc aaaacgacaa
     4201 actgccttct gccaacaagc tggtggctaa cggtgtattg gcactcaatg cgcgctattc
     4261 ccaaattgcc gacaacacca cgctgagagc gggtgcaatc aaccttactg ccggtaccgc
     4321 cctagtcaag cgcggcaaca tcaattggag taccgtttcg accaagactt tggaagataa
     4381 tgccgaatta aaaccattgg ccggacggct gaatattgaa gcaggtagcg gcacattaac
     4441 catcgaacct gccaaccgca tcagtgcgca taccgacctg agcatcaaaa caggcggaaa
     4501 attgctgttg tctgcaaaag gaggaaatgc aggtgcgcct agtgctcaag tttcctcatt
     4561 ggaagcaaaa ggcaatatcc gtctggttac aggagaaaca gatttaagag gttctaaaat
     4621 tacagccggt aaaaacttgg ttgtcgccac caccaaaggc aagttgaata tcgaagccgt
     4681 aaacaactca ttcagcaatt attttcctac acaaaaagcg gctgaactca accaaaaatc
     4741 caaagaattg gaacagcaga ttgcgcagtt gaaaaaaagc tcgcctaaaa gcaagctgat
     4801 tccaaccctg caagaagaac gcgaccgtct cgctttctat attcaagcca tcaacaagga
     4861 agttaaaggt aaaaaaccca aaggcaaaga atacctgcaa gccaagcttt ctgcacaaaa
     4921 tattgacttg atttccgcac aaggcatcga aatcagcggt tccgatatta ccgcttccaa
     4981 aaaactgaac cttcacgccg caggcgtatt gccaaaggca gcagattcag aggcggctgc
     5041 tattctgatt gacggcataa ccgaccaata tgaaattggc aagcccacct acaagagtca
     5101 ctacgacaaa gctgctctga acaagccttc acgtttgacc ggacgtacgg gggtaagtat
     5161 tcatgcagct gcggcactcg atgatgcacg tattattatc ggtgcatccg aaatcaaagc
     5221 tccctcaggc agcatagaca tcaaagccca tagtgatatt gtactggagg ctggacaaaa
     5281 cgatgcctat accttcttaa aaaccaaagg taaaagcggc aaaatcatca gaaaaaccaa
     5341 gtttaccagc acccgcgacc acctgattat gccagccccc gtcgagctga ccgccaacgg
     5401 tatcacgctt caggcaggcg gcaacatcga agctaatacc acccgcttca atgcccctgc
     5461 aggtaaagtt accctggttg cgggtgaaga gttgcaactg ctggcagaag aaggcatcca
     5521 caagcacgag ttggatgtcc aaaaaagccg ccgctttatc ggcatcaagg taggcaagag
     5581 caattacagt aaaaacgaac tgaacgaaac caaattgcct gtccgcgtcg tcgcccaaac
     5641 tgcagccacc cgttcaggct gggataccgt gctcgaaggt accgaattca aaaccacgct
     5701 ggccggtgcg gacattcagg caggtgtagg cgaaaaagcc cgtgccgatg cgaaaattat
     5761 cctcaaaggc attgtgaacc gtatccagtc ggaagaaaaa ttagaaacca actcaaccgt
     5821 atggcagaaa caggccggac gcggcagcac tatcgaaacg ctaaaactgc ccagcttcga
     5881 aagccctact ccgcccaaat tgtccgcacc cggcggctat atcgtcgaca ttccgaaagg
     5941 caatctgaaa accgaaatcg aaaagctgtc caaacagccc gagtatgcct atctgaaaca
     6001 gctccaagta gcgaaaaaca tcaactggaa tcaggtgcag cttgcttacg acagatggga
     6061 ctataaacag gaaggcctaa ccggagccgg agccgcaatt atcgcactgg ccgttaccgt
     6121 ggtcacctca ggcgcaggaa ccggagccgt attgggatta aacggtgcgg ccgccgccgc
     6181 aaccgatgca gcattcgcct ctttggccag ccaggcttcc gtatcgttca tcaacaacaa
     6241 aggcgatgtc ggcaaaaccc tgaaagagct gggcagaagc agcacggtga aaaatctggt
     6301 ggttgccgcc gctaccgcag gcgtagccga caaaatcggc gcttcggcac tgaacaatgt
     6361 cagcgataag cagtggatca acaacctgac cgtcaaccta gccaatgcgg gcagtgccgc
     6421 actgattaat accgctgtca acggcggcag cctgaaagac aatctggaag cgaatatcct
     6481 tgcggctttg gtgaatactg cgcatggaga agcagccagt aaaatcaaac agttggatca
     6541 gcactacata gtccacaaga ttgcccatgc catagcgggc tgtgcggcag cggcggcgaa
     6601 taagggcaag tgtcaggacg g
//
LOCUS       DAXIHS010000094         6591 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXIHS010000094 DAXIHS010000000
VERSION     DAXIHS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.094"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(215..2011)
                     /locus_tag="ACFOF2_001784"
     CDS             complement(215..2011)
                     /locus_tag="ACFOF2_001784"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC7108051.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGTNPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2469..3722
                     /locus_tag="ACFOF2_001785"
     CDS             2469..3722
                     /locus_tag="ACFOF2_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC7108052.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3877..5388
                     /gene="lysS"
                     /locus_tag="ACFOF2_001786"
     CDS             3877..5388
                     /gene="lysS"
                     /locus_tag="ACFOF2_001786"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC7108053.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHTQYGEIGKEELDPQGIPVKIAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5465..5653)
                     /locus_tag="ACFOF2_001787"
                     /pseudo
     CDS             complement(5465..5653)
                     /locus_tag="ACFOF2_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5657..6535)
                     /locus_tag="ACFOF2_001788"
     CDS             complement(5657..6535)
                     /locus_tag="ACFOF2_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC7108054.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
BASE COUNT         1421 a         1709 c         1879 g         1582 t
ORIGIN      
        1 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
       61 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
      121 atttgcttgc ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa
      181 gggctttcag acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt
      241 tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg
      301 cgtggtagcg gttgacccag tcgatttcgc cgtcggtcat gagggcggtg tccatcaggc
      361 gggtgtcgat ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt
      421 cttgaggggc ggcgacggct tggttggcgg caaggttttc aatgcggatg ccccattttc
      481 ccgggcggta gagtccgggt tcgatggagg taaccatgcc ttttttcatg gcggtttcgg
      541 gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc
      601 ctacgccgtg tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc
      661 aaatcgcatc aatcagcggc gaggggatgt tttctgggaa cacggcttcg gcaagcgcga
      721 tatgggcttt gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag
      781 ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagca
      841 gcagcccgtt gccgctgatg gtgctgtggc tttcgggcgt tgcgctgtaa tgcggcaggg
      901 cgccgttggc gttgaagcct gcgatggtgt cgaaactcaa tgacacaaaa cccgggcgca
      961 cgctgcggtg gcggtaaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc
     1021 cgatgatgtc ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg
     1081 cttcgcggat gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt
     1141 tggtgccttc gattaggcgt acgctttcgg gcaggcgcac gagcgtactg acggcggttt
     1201 tgttcggttc gataagcagc gcgccgccga tttgcgcgag cttgtcggca acttgggcgt
     1261 aaggttcgac cgtgatgccg gcggtttgca gcgcggcggc ggcttcggcg ttcagacggc
     1321 attgttcggt aaacaggacg gcgttgtctt tgccaatcag caggaaggac acgaaaacgg
     1381 gattgaaagg cacgtcgctg ccgcgcaggt tggtcagcca agcgatgtcg tcaagcgatg
     1441 aaaccaagtg gtaatccgcg cctttttccg ccatcacggc gcgcacgcgg gcgagttttt
     1501 cggcggcggt ttcggagacg taggcggggt cgtggatgaa cacggtttcg gcggggatag
     1561 cgggacggct tgtccacact tggtcaagca gatcgtccgg gtgttcgatg cggatgtttt
     1621 tggcggtcag ggattgcgcc aaagtgcgtt tgccggtgag cgagaccata tcggaaggga
     1681 tgccgacggc ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt
     1741 gcccgctttt ttgcagttcg atgccgctgc ccgcaagctg cttggcggct tgttcccaat
     1801 agcggctgtc cacccatacg cccgcttcat cggtggtcag gacaaacgtg ccgaccgagc
     1861 cggtaaagcc cgataattcg cggcgcgcct gccaatgctc gggcaggtat tcggacaggt
     1921 gggggtcggc ggaagggatg acgagcgcgt ccaagccttg cgccttcatg gcttcgcgca
     1981 atgcggacag gtaattcgat acggtattca taacagttcc tccaatcggg ttttgcggct
     2041 tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat
     2101 cgccagcctg ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaaagc
     2161 gatgtaaggt tgtttgttgt aaaattttag gcataaaatg tcgttttatg cctttttttg
     2221 gaagtaatat ggaaaatata gtcaagtttg agcatgaagt tctaatagtg tgtgttggca
     2281 ggtatttgga ttacgttaca atacgtgttt tcatcaaaat ttccctaccg agcggtatcc
     2341 tgtttttgaa gacggtagga tttgggtttg tttgggggca atcccgccca aattggagca
     2401 atagataggg ctgtgtccga atatgcggct ctgtgtgttg aaacacagtt aaaaatacgg
     2461 aagactttat gtccgaacaa catatttcga cttggaaaag taaaatcaac gcattgggtc
     2521 cggggatcat gatggcttcg gcggcggtcg gcggttcgca cctgattgcc tcgacgcagg
     2581 cgggcgcgct ttacggctgg cagatcgcgc tcatcatcat cctgaccaac ctcttcaaat
     2641 acccgttttt ccgcttcagc gcgcattaca cgctggacac gggcaagagc ctgattgaag
     2701 gttatgccga gaaaagccgt gtttatttgt gggttttcct gattttgtgc atcatttccg
     2761 ccacgattaa cgctggcgcg gtcgccatcg taaccgccgc catcgtcaaa atggcgattc
     2821 cctcgctgat gtttgatgcc ggcacggttg ccgccttgat tatggcatcc tgcctgatta
     2881 ttttggtgag cggacgttac cgcgctttgg atcgcgtttc caaaatcatc atcgttactt
     2941 tgagtatcgc cacgcttgcc gccgccggca tcgctatgtc gcgcggtatg cagatgcagc
     3001 cggactttat cgagccgaca ccgtggacgc ttgccggttt gggcttcctg atcgcgctga
     3061 tgggctggat gcccgcgccg attgaaattt ccgccatcaa ttctttgtgg gtaaccgaaa
     3121 aacaacgcat caatccttcc gaataccgcg acgggatttt tgatttcaac gtcggttata
     3181 tcgccagtgc ggttttggct ttggttttcc ttgcactggg cgcgtttgtg caatacggca
     3241 acggcgaagc agtgcagatg gcgggcggca aatatatcgg gcaattgatc aatatgtacg
     3301 ccgttaccat cggcggctgg tcgcgcccgc tggtggcgtt tatcgctttt gcctgcatgt
     3361 acggcacgac gattaccgtt gtggacggct atgcccgtgc cattgccgaa cccgtgcgcc
     3421 tgctgcgcgg aaaagacaaa acgggcaacg ccgaattctt tgcctggaat atttgggtgg
     3481 cgggcagcgg tttggcggtg attttttggt ttgacggcgt aatggcgaat ctgctcaaat
     3541 ttgcgatgat tgccgctttt gtgtccgccc ctgtgtttgc ctggctgaat taccgtttgg
     3601 tcaaaggtga tgaaaaacac aaactcacat caggtatgaa tgcccttgca ttggcaggct
     3661 tgatttatct gaccggcttt accgttttgt tcctattgaa tttggcggga atgttcaaat
     3721 gataacgatg ccgtctgaaa ccgcaaaccg ctttcagacg gcattgccgc gtttatggaa
     3781 aaaaacaccg acatcgcgta aaatatgcgc aaattttgtg attcggtcag gtcgtctgaa
     3841 acagattgcg cctgatttat tttttcggaa aaccctatga gcgaacaaaa ccatccgcaa
     3901 accgagccgc agttggacga aaaccaaatc atcgccctgc gccgcgaaaa actgcacaac
     3961 atccgccaac agcgcaacgc ctaccctaac gatttcaaac gcgacagctt tgccgccgac
     4021 ctgcataccc aatacggtga aatcggcaaa gaagaactcg acccgcaagg cattcccgtc
     4081 aaaattgccg gccgcatgat gctcaaacgg caaatgggca aggcgagctt tgccaccatt
     4141 caagacgtgt ccgggcaaat ccagctttat ctgaacaaca aaggcgtgag ccaagaaatt
     4201 ttggacgact tcaaccattg ggacttgggc gacatcgtcg gtgcggaagg cactttgttc
     4261 aaaaccaacc acggcgaact gaccgtacgc gtgtccggca tccgcctgct gtccaaatcc
     4321 ctgcgtccgc tgcccgacaa acacaaaggt ttgagcgatc aggaaaccaa ataccgccaa
     4381 cgctatgttg atttgattgc caacggagaa tcgcgcaata ccttcatcaa acgcagccaa
     4441 atcatccaat ccgtgcgtaa ttttatggtg ggcgagcatt atctcgaagt cgaaaccccg
     4501 atgatgcacc cgattcccgg cggcgcgacg gcaaaaccct tcgttaccca ccacaatgcc
     4561 ttggacattc cgctctacct gcgcatcgcc cccgagctgt atctgaaacg cttggttgtc
     4621 ggcggtttgg agcgcgtgtt tgaaatcaat cgcagcttcc gcaacgaagg tatgtccgtg
     4681 cgccacaatc ccgaattcac catgatcgaa ttctacgaag ccttctccga ctacgaacgt
     4741 atgatgcaga tggcagaaga catcatccgc aacgcatcgc gcacagtaaa cggtacggca
     4801 aacatcactt acaatggcaa agaagtcgat ttggaaagcc cgttcgagcg tctgaccatc
     4861 ctcgaagcca tcaaaaaata caatccgcac tacaccgacg agcagttgaa cgatgcggaa
     4921 tggctgaaaa aagaaatcgt caaacacggt gaaagcctgc cgccgtcccc gggcatcggc
     4981 agcctgcaac tcgcgctgtt tgaaggttgc gccgagggca agctgtggaa cccgaccttc
     5041 atcatcgatt acccggtcga agtttccccg ttggcgcgcg cttcggatac caaacaaggt
     5101 ctgaccgaac gtttcgaatt gttcgttgtc ggccgcgaac tggcaaacgg ctattcagaa
     5161 ttgaatgacc ctgaagacca agccgaacgc ttcaaatcgc aagtcgcaca aaaagacgcg
     5221 ggcgacgacg aagccatgca ttacgatgcc gactacatcc gcgcgatgga atttggtttg
     5281 ccgccgaccg gcggttgcgg catcggtatc gaccgcttgg tgatgttgct gaccgattcg
     5341 caaaccatcc gcgatgtgat tctgtttccg cagatgcgcc ccgagtagtc ataaaaacag
     5401 ttgaaatggc aatgccgtct gaacccgatt ggattcagac ggcattttgt atggcggtac
     5461 ggatttattc gctttctaac tgaccgaccc attcggacaa ggcagtcagg cgttgttcgg
     5521 atttcgccac gaacgggttt tcggtatccc acgcgtagcc tgccaaaatc gaagaaagca
     5581 tacggctgat ttccgggctg cggtcgggcg cgtacacgac gttttggaag cggaaatcgt
     5641 accaaccgtc cacacatcag gggtggtaat ccgaatcaac acgccttctc gcctgtgcgg
     5701 cggacataaa ccgttttttg acctccagca ccacacctaa aatcgtgctg ccttcgggca
     5761 ggtcaaaggc ttcagacggc ctgcccgtct gataaatcag catcctgccc gcaatatcga
     5821 taacaaggtg cgcgacgacc agccgcttgt cggacagttc aatcagcacg atgtcctcgt
     5881 ccgcagggcg catacgcggc tcgaccacca ccaaatcccc tttttcgata accggcgaca
     5941 tggacgtgtc gggcatggca acggcaaacg tgtactgcga gggggcgacc accgaagcaa
     6001 acaagtcgaa actgcctgtg agtttgcctg tacgggcgaa agccgccgcc tgacggtctg
     6061 acaacagggg gcgtggtgtg agcctcatcg acagtgcttt gccgtcggca tcgctgtggt
     6121 tgtgcgtatg cgtatgatgg ttgttgatgg tcgtatcgcc ttggacatcg ttaaggacta
     6181 tgctgtttga acctgccacg gatttggtca tctcattgta ggcttgcgtc gtgatgcgct
     6241 cttgcttggt catacctgtt acagcctgtt tcagcatttc taacgctatg tgattcttcc
     6301 taagggtcgc tataccggct tttgcagctt gctcattacc acaagagggg caaccccagc
     6361 tacttcctat gaacatattg gagtacctcg aacaggtttg gtttccatgt ttagggcagt
     6421 tgattacaca cggcttggct gtaccctcaa actcgaccaa cttaatgcca cgatgaccga
     6481 aacgttcctc aacctcagca gcagcttctt caaatgtttt tgaccttctt gccatattga
     6541 cctccggatt attgattatt ttggctatgc tttaacgagg gattggaatg a
//
LOCUS       DAXIHS010000095         6439 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXIHS010000095 DAXIHS010000000
VERSION     DAXIHS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.095"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(116..799)
                     /locus_tag="ACFOF2_001789"
     CDS             complement(116..799)
                     /locus_tag="ACFOF2_001789"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC7108055.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1095..3398
                     /gene="parC"
                     /locus_tag="ACFOF2_001790"
     CDS             1095..3398
                     /gene="parC"
                     /locus_tag="ACFOF2_001790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC7108056.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTI
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            3418..4994
                     /locus_tag="ACFOF2_001791"
                     /pseudo
     CDS             3418..4994
                     /locus_tag="ACFOF2_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            4987..5454
                     /locus_tag="ACFOF2_001792"
     CDS             4987..5454
                     /locus_tag="ACFOF2_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC7108057.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            5558..6310
                     /locus_tag="ACFOF2_001793"
     CDS             5558..6310
                     /locus_tag="ACFOF2_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC7108058.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1568 a         1997 c         1596 g         1278 t
ORIGIN      
        1 cagaaaaaca aaaacctaaa atcccgtcgt tctcgcgaaa gcagttgcta cgtttaccgt
       61 caaaaatgcc gtctgaacgc tcaagccttc agacggcatt ttttatttca cacccttaca
      121 ggtagaattt ttcgatgact ttcaaattgt cgtccaattt gtacaccaac ggctgaccgg
      181 tcgggatttc caagcccatg atgtcttcgt cggaaatgcc ctcgatgtgt tttgccagcg
      241 cgcgcaggga gttgccgtgc gccgccacca agacgcgttt gccgctcaaa atcgcggggg
      301 cgatttggtc ttcccaaaac ggcaatacgc gctccagcgt tacttgcagg ttttcgccgt
      361 cggggataac atcggcaggc agatgggcat agcggcggtc tttgtgtgcg gaaaactcat
      421 cgtctttgtc caaaagcggc ggcagggtgt cgtagctgcg ccgccagatg cggacttgct
      481 cgtcgccgta ttgttcggcg gtttgttttt tgtccaggcc ttgcagtcgg ccgtagtggc
      541 gttcgttcag ccgccatgtt ttgatttgcg gtacgaacag ttggtcggat tcttccaaaa
      601 cgatgttgca ggtcttaatc gcgcgggtca ggacggatgt gaaggcgatg tcgaactcat
      661 agccgttttc tttcagtttt ttaccggcgg cagcagcctc ggcaagcccc tgctcgctca
      721 gcttcacgtc gcgccagcct gtaaacaggt ttttcgcgtt ccattcgctt tgtccgtggc
      781 ggataaatac caattccata tcgtctccaa tgtgtgaaag tgggaaagcc ttatttataa
      841 catattttca cattcccgta tttgattcag attcagacac gcgcccacta tggtttgccg
      901 ttttgattta caataatgcc ctttgcttta cattccgcat acacaatgtt tcaacacaca
      961 ggacgacaca taaagcaccg ccctatgtgt tgccctgatt tggaaggggt tgccccctcc
     1021 caaataaagt ctgattctac cgccccgaag gacagatgtc caagtggcgg gggttcaacc
     1081 gaaaaggaaa tacgataaat atgcaaccgc acgctccaca taccgattcc aatacgctga
     1141 tgctcggccg atacgccgaa cgcgcctatc tcgaatacgc catgagcgtg gtcaaaggcc
     1201 gcgcgctgcc tgaagtttca gacggccaaa agcccgttca gcggcgcatt ttgtttgcca
     1261 tgcgcgatat gggtttgacg gcgggggcga agccggtgaa atcggcgcgc gtggtcggcg
     1321 agattttggg taaataccac ccgcacggcg acagttccgc ctatgaggcg atggtgcgga
     1381 tggcgcagga ttttaccttg cgctatccct taatcgacgg catcggcaac ttcggttcgc
     1441 gcgacggcga cggtgcggcg gcgatgcgtt acaccgaagc tcggctgacg ccgattgcgg
     1501 aattgctgtt gtccgaaatc aatcagggaa cggtggattt cgtgccgaac tacgacggcg
     1561 cgtttgacga gccgctgcac ctgcccgccc gcctgcctat ggtgttgctc aacggcgcgt
     1621 caggcatcgc ggtgggcatg gcgaccgaga ttccgtcgca caatttgaac gaagtgacgc
     1681 aggcagcgat tgcgctgttg aagaagccga cgctggaaac cgccgacctg atgcaatata
     1741 ttcctgcgcc cgattttgcc ggcggcggtc aaatcatcac gccggcggac gaattgcgcc
     1801 ggatttacga aaccggcaaa ggcagcgtgc gcgtgcgtgc gcgttatgaa atcgagaagc
     1861 tggcgcgcgg acagtggcgc gtcatcgtaa ccgagctgcc gccgaacgcc aattccgcca
     1921 aaatccttgc cgaaatcgaa gaacaaacca acccgaaacc gaaagcgggt aagaaacagc
     1981 tcaaccaaga ccagctcaac accaaaaagc tgatgctgga tttaatcgac cgcgtgcgcg
     2041 acgagtccga cggcgaacat cccgtgcgac tggtgttcga accgaaatcc agccgcatcg
     2101 ataccgatac cttcatcaac acgctgatgg cgcaaacttc gctggaaggc aatgtgtcca
     2161 tgaacttggt gatgatgggg ctggacaacc gccccgcgca gaaaaacctg aaaacgattt
     2221 tgcaggaatg gctggatttc cgcaccgtaa ccgtaacacg ccgtctgaaa ttccgtttga
     2281 accaagtgga aaaacggctg cacatcctcg aaggccgtct gaaagtcttt ctgcacatcg
     2341 acgaagtgat taaagtcatc cgcgaatcag atgacccgaa aaccgatttg atggcggcgt
     2401 tcgggctgac cgaaatccaa gccgaagaca ttttggaaat ccgcctgcgt cagttggcgc
     2461 gtttggaagg tttcaaactc gaaaaagaat tgaacgaatt gcgcgaagaa caaggccgtc
     2521 tgaacatcct tttgagcgac gaaaacgaaa aacgcaagct gattgtcaaa gagatgcagg
     2581 cggacatgaa acagttcggc gacgcgcgcc gcacgctggt ggaagaagcc ggacgcgccg
     2641 tgctgacaca aaccaccgcc gacgaaccca tcacactgat tctgtcggaa aaaggctgga
     2701 tacgcagccg cgccggacac aatctcgatt tgagccaaac cgcgttcaaa gaaggcgacc
     2761 gcctcaaaca aaccctcgaa ggcagaacga ttttacccgt cgtcattctc gattcatcgg
     2821 gcagaaccta cacccttgat gccgccgaaa tccccggcgg gcgcggcgac ggcgtgccgg
     2881 tttcatcctt gatcgagctg caaaacggcg cgaaacccgt tgcgatgttg acaggattgc
     2941 cggaacaaca ttatttatta tcaagcagcg gcggctacgg cttcatcgcc aaactgggcg
     3001 atatggtcgg gcgcgtgaaa gcgggcaaag tggtgatgac cgcagacagc ggcgaaaccg
     3061 ttttgccgcc ggttgccgtc tatgcctcct cgttcatcaa ccccgactgc aaaatcattg
     3121 ccgccaccaa tcaaaaccgc gccctcgcct tccccatcgg cgaattgaaa attatggcga
     3181 aaggcaaagg gctgcaaatc atcggattaa acgccggcga atcgatgacg cataccgccg
     3241 tttcttccga gccggaaatc ctgattgaaa gcgaaggcag gcgcggcgca gcgcacaaag
     3301 accgcatccc catctccctg cttgaggcaa aacgcggcaa aaaaggcaaa ctattaccca
     3361 tatcgggcag cctgaaacag ctttcttccc cgaaataaac ccgactctgc acatactatg
     3421 gtaatttcca atacccgcga acttgagaaa ctcaaagacc ggattcccaa tctgatcaac
     3481 atcgcccgca tcgccatcgt cttccccctg ctgattatgc atatcttcgg gttggaggtc
     3541 agcggacatg aaaacctgca cgcttcgtgg acggcgtggg cattttatgt ttggctcgcc
     3601 atttcctgct ggctgatttt cttttccatc atccatccgc attggcaatg gcagtcgctg
     3661 aaaatgccgc gtttcagcgc ggtggcggac atcacgatga tcggcgtgct gacctacctg
     3721 ttcggcggca tcgattccgg cttcggcatc ctgattctgc ccttcgtcgt ctgctcctgc
     3781 ctgctcagct acgggcgtta ccccctgctc tatgccagct acgccgccat cctgctgata
     3841 ttcaacgcca ttgccgacgg cgatatcggc aaatatccgc acatatcgga tgcccgaacc
     3901 gcctcggcaa ccttcatcct cgtcgccgcc tcctatcttt ccgccatctt cacctcactg
     3961 tcggtcagat acatcgacag cgccggcaaa cttgcccatg aaaaccacgc cgcctaccgc
     4021 cgcatcaggg gcttgaacca aatcgtactc aaccgcgttc aggaagcggt cgtcgtcatc
     4081 aacgtcgagc atcagaccat actgttcaat aaaaaggcaa aagatctgct ccccatgctt
     4141 gaaatcggac agcataccgc cctattcgac cctattaccg ttttatggga caaggcctct
     4201 tcgcgtactt tcgaacgcaa tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg
     4261 ccgatgaaca aagagcaaaa caaactgctc atcctctaca tccgcccgca aagcgaaatt
     4321 caggcagaag ccctgtccgt caaacttgcc gcactcggac aactgaccgc caacctcacc
     4381 cacgaaatcc gcaacccgat gtccgccatc cgccacgcca acggcctgct gcgcgaaaat
     4441 atggaagcgg gggcggcaga tccgttcaac gccaaattgt gcaaaatcat cgacggcaac
     4501 gtctgccgta tcaacaaaat gctcgaagat atttcctcgc tcaacaagcg caacaaaacc
     4561 gaacgcgaag ccatcggcct gataccgttt tgggaagaat tcaaacaaga gttcctgctc
     4621 ggccatcccg atgccgccgg ctgcatccgc ctagatatgc agggcaatca cctgaccgcc
     4681 tatttcgatc ccgcccacct gcggcaaatt atgtggaacc tcgccaacaa cgcgtggcgg
     4741 cacagccgca aacagcccgg ctcgatttcc gtcaccatcc gccccgcgca aaaaaacacc
     4801 gtctgtatcc tctttgccga ccgcccgaag tgcaggaaca cctgttcgaa cccttttaca
     4861 ccacggcgga aaacggcacc ggcctcgggc tgtatgtcgc ccgcgaactg gcgcacgcca
     4921 atttcggcga tttgacctac ctaccggaag ccaaatgttt cgaactcaca ttaccggaaa
     4981 aaaccaatga ctgaactgca acaccccgtc ctcgtcgtcg atgacgaaac cgacattctc
     5041 gacctgatgg aaatgaccct gatgaaaatg ggcttgcgcg tccataccgc gtcaggcgtt
     5101 gccgaagcca aaaacaagct cgacagccaa cgctattcgc tcgtcctgac cgatatgcgt
     5161 atgccggacg gctcggggct ggaagtcgtc caacacatca acagccgcct gctcgatacg
     5221 ccggttgccg tcatcaccgc cttcggcaac gccgatcagg cacaggaagc gttgcgttgc
     5281 ggcgcgttcg accccgatac catgcagata caggactatc tcgaccaaat cgaacgcgac
     5341 attatcgaac aaaccctcaa acaaaccgaa ggcaaccgca cgcaggccgc caaacgcttg
     5401 ggcatcagct tccgttccat gcgctaccgt atggaacgcc tcaacatcgg ctgacgacaa
     5461 aacggcatcc gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg gcacgggaaa
     5521 gcgggttcgc cccacgcccg aacggacaca aaacaccatg accgacatcc ttattgacaa
     5581 caccgccacc gaaaccgtcc gcaccctgat acgggcattc ccccttgtgc ccgtttccca
     5641 accgcccgaa caaggcagtt acctccttgc cgaacacgat accgtcagcc tcaggcttgt
     5701 cggggaaaaa agcagcgtca tcgtcgattt tgcctccggc gcggcacaat accggcgcac
     5761 aaaaggcggg ggcgaactca tcgccaaagc cgtcaaccac accgcgcacc ccaccgtttg
     5821 ggacgcaacc gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg ggctggccgt
     5881 taccgccttc gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca tccgccgcgc
     5941 cctcctcaat cccgaaacgc aagacaccgc cgcgcgcatc accctgcatt tcggcaacgc
     6001 cgcagtacaa atgcccgcac ttgttcaaac acaaggcaaa cccgacatcg tctatctcga
     6061 ccccatgtat cccgaacgcc gcaaaagtgc cgccgtcaaa aaagaaatga cctacttcca
     6121 ccggctcgtc ggtgaagcgc aagatgaggc cgccctcctc cataccgcac gccaaacggc
     6181 aaaaaaacgc gtcgtcgtca aacgcccccg cctcggcgaa caccttgccg gacaagcccc
     6241 tgcctaccaa tacaccggca aaagcacccg cttcgacgtt tacctgccct acgggacgga
     6301 caagggataa cgcccataaa acaagacacc gaaaaatttg ccgttcttat gcaaacgaga
     6361 aaccggtttt tgcgtttcga ctgttttgga taaatcatca caccttaaag tttgtcattc
     6421 ccacggaagt gggaatcca
//
LOCUS       DAXIHS010000096         6174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXIHS010000096 DAXIHS010000000
VERSION     DAXIHS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.096"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(110..697)
                     /locus_tag="ACFOF2_001794"
     CDS             complement(110..697)
                     /locus_tag="ACFOF2_001794"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC7108059.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            860..2266
                     /gene="dnaB"
                     /locus_tag="ACFOF2_001795"
     CDS             860..2266
                     /gene="dnaB"
                     /locus_tag="ACFOF2_001795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC7108060.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2568..3236
                     /locus_tag="ACFOF2_001796"
     CDS             2568..3236
                     /locus_tag="ACFOF2_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC7108061.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSRFVYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            3266..3889
                     /gene="pilV"
                     /locus_tag="ACFOF2_001797"
     CDS             3266..3889
                     /gene="pilV"
                     /locus_tag="ACFOF2_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC7108062.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGAAPTLSAGGAFSQNCD
                     GSANGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            3886..4821
                     /locus_tag="ACFOF2_001798"
     CDS             3886..4821
                     /locus_tag="ACFOF2_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC7108063.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIGSDVSNVAQKKPLF
                     SLKRSSTNKLIPIAESSNIGYQGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIVKP
                     GKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQW
                     GNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVLLS
                     SGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            4800..5393
                     /locus_tag="ACFOF2_001799"
     CDS             4800..5393
                     /locus_tag="ACFOF2_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC7108064.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTAKSTGLCIDNKGMEYKKGTRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5398..5895
                     /locus_tag="ACFOF2_001800"
     CDS             5398..5895
                     /locus_tag="ACFOF2_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC7108065.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQIILKNPQDSNDILNIKLTRFVSGYKMNPKIAEKYSVSGEFVDAPKSRA
                     SRALKSRAYRLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASARAYSETLSADAGCEAF
                     SNRKK"
BASE COUNT         1699 a         1356 c         1592 g         1527 t
ORIGIN      
        1 cccacggaag tgggaatcta gaaataaaaa gcagcaggca tttatcggaa ataactgaaa
       61 ttcaataccg caaaaatcta cccgaaatga tatagcggat taacaaaaat caggacaagg
      121 cggcaaaacg tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt
      181 taggacggct gttgcggtag tcgatgtagt aggcgtgttc ccacacgtcg caggtcagca
      241 gcggcgtgtt ttcagtggtc agcggcgtag cggcgttgga agtggaaacc aaatccaatc
      301 cgccggcagg ggtttttacc agccacgccc aaccggagcc gaaagtaccg gccgcgcagg
      361 cattgaacgc ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca
      421 gttcgccgga aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaagtgtggt
      481 tccaagtttg ggctgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca
      541 aaggcaggtt ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt
      601 gatggtgttt gccgtagtgg aattccaaag tttctttgct cagatgcggg gacaatgcgt
      661 ccagttcata aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta
      721 aatgttgtat tttggcagtg ttgctgcaaa tagctcagca gcgcacatat tttacctgtt
      781 ttgagggatg gaaaccaatt aaacctgctt tacgctataa tagaaggttg caatttcggc
      841 acgacagata ggatgtacca tgaacgatta cacagctatg ccgtctgaag accgtgaggt
      901 cggcgcattg tcgctgcctc cacattcgat ggaggcggaa caatccgttt tgggcggttt
      961 gatgctggaa aatccggctt gggacaggat tgccgatgtg gtttccggtg aagacttcta
     1021 ccgccacgaa caccgcctga ttttccgatc cattgctaaa ttaatcaatg agagccgtcc
     1081 cgccgatgtg attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg
     1141 cggattcgaa tatctgatta cgctggcgca aaacaccccg tctgccgcca acatccgccg
     1201 ctacgccgaa atcgtgcgcg agcgttccat catgcgccaa ctcgccgaag tggggacgga
     1261 aatcgcccgc agcgcataca atccgcaagg cagggacgcg gggcagcttt tggacgaggc
     1321 ggaaaacaaa gtattccaaa tcgccgaaag caccgccaaa tccaagcagg gctttttgga
     1381 gatgcccgat ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa
     1441 tcccgatgaa gttaccggcg tgccgacagg gttcatcgac ctcgacaaaa aaacctcggg
     1501 tctgcaaccc ggcgacctga ttatcgttgc cggtcgtccg tctatgggta agaccgcctt
     1561 ttctatcaat atcgccgaac acgttgccgt agaaggcaag ctgccggttg ccgtcttctc
     1621 aatggaaatg ggcggggcgc aactggtcat gcgtatgctc ggctcggtcg gacggttgga
     1681 tcaaagcgtt ttgaaaaccg gcaggctcga agacgaacac tggggtcgcc tgaacgaagc
     1741 agtcgtcaaa ctctccgacg cgcccgtgta catcgacgag accccgggtc tgaccgcgct
     1801 cgaactgcgc gcccgtgccc gccgtctcgc ccgtcaattt aacaataagc ttggattgat
     1861 tgtcatcgac tacctgcaac tgatggcagg atccggccgt tccgacaacc gagcttcgga
     1921 gctgggagag atttcacgtt cgctcaaagc gttggcgaaa gaattgcaag tccccatcat
     1981 cgccctgtcg caattgagcc gcacggtcga atcgcgtacc gacaaacgcc cgatgatgtc
     2041 cgaccttcgc gaatccggtg ctatcgagca ggatgccgac ctgattatgt ttatgtaccg
     2101 cgacgaatac tacaaccaag actcgcccat gaaaggcctt gccgaatgta tcatcggcaa
     2161 acaccgcaac ggtcccgtcg gtaaaatctt cctcacatgg acgggacaat tcaccaaatt
     2221 cgacaatgct gcctatattc ccgaggaggc aaagatagag gattaaatgg ctatataaaa
     2281 atttattagg cgaaatcagg caaaatcgtt taaaatcatg cttagagatt gccctaaaaa
     2341 ataaaacgcg gtcttgaggc atttttgcat tcagcccgca tataattgaa aatatagtgg
     2401 attaacaaaa atcaggacaa ggcaacgaag ccgcagaccg tacaaatagt acggaaccga
     2461 ttcacttggt gcttgagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc
     2521 cgtactggtt tgaatttaat ccacgataca ttaccagtta acgtcctatg tgtacacgaa
     2581 aacaacaagg tttcacgcta acagagctgc tcatcgtgat ggtcattgca gccattatgg
     2641 cgatgatagc cctccccaat atgagccaat ggattgcatc ccgccgcatt gccagtcatg
     2701 cggagcggat tgccaacctt ttgcgtttct ccaggggaga agccgtccgg ctcaatcttc
     2761 ctgtctatat ctgtcctgtt caagttaaaa aaggcggtgc gcccaacaat aaatgtgact
     2821 tcggcaagaa ggggcaggga atgttggctt ttggcgataa gaatggcaat aagacatatg
     2881 acggcgatac ggcggatgtt ctccttcgca gtgtggtatt gaatgatgat atcaatgata
     2941 agcggattaa ttatgccttt aaccatatcg ctttcggtca aacgcagccg accgctgacc
     3001 gtgtagtttg gacattcaat caaaacggga cgttcggtta ttctaccaat caggatctca
     3061 cgaatacttc cagatttgtt tactccgacg gttatatcca aatcgtgctg acagatgcga
     3121 gagcagtttc agatgccgat aagaaattcc gttcggcggt ggttttgatt aacagcagcg
     3181 gcagggttga ggtttgtcct agaaacgata ggcgtaccgt atgccaatat aaatgattgt
     3241 tttagtcggt ttgggaaata aaaaaatgaa gaataatgat tgcttccgcc tgaaaaaccc
     3301 ccagtccggt atggcgctga tagaagtctt ggttgctatg ctcgttctga ccatcggtat
     3361 tttggcgtta ctgtctgtgc agttgcggac agtcgcttcc gtcagggagg cggagacaca
     3421 aaccatcgtc agccaaatca cgcaaaacct gatggaagga atgttgatga atccgaccat
     3481 tgattcggac agcaacaaga aaaactataa tctttacatg ggaaacaata cactatcgca
     3541 tacactatca gctgtggatg gtgagtttgc gattgatgcc ataaaaacta agacacagtt
     3601 ggcagaggca caattgaaga gatttagtta tgagctgaaa aatgccttgc cggatgcggc
     3661 agccatccat tacgccgtct gcaaggattc gtcgggtgct gcgccgacat tgtccgccgg
     3721 cggtgctttt tctcaaaatt gcgatggtag tgcaaatggg gatactttga ttaaagtatt
     3781 gtgggtaaat gattcggcag gggattcgga tatcgcccgt acgaatcttg agacgaacgg
     3841 caacaatatc gtatatacct atcaggcaag ggtcggaggt cgggaatgag acgtaaaatg
     3901 ctaaacgtac caaaaggcag ttatgatggt atgaagggtt ttaccattat tgaatttttg
     3961 gttgcgggca tgctcagtat gattgtcctg atggcggtcg gatcgagtta cttcacgtcc
     4021 cggaaattaa atgatgcggc aaacgagcgt cttgctattc aacaggattt gcggaatgcg
     4081 gcaacattaa ttgtccgcga tgcaagaatg gcggggagct tcggttgttt caatatgtct
     4141 gagcatatcg gaagtgatgt ttccaatgtg gcgcaaaaaa aacctctttt ttccttaaaa
     4201 aggagcagta caaataaact tatccccata gcggaatctt caaatatcgg atatcagggt
     4261 tttacccagc gccttaacgc attgattttc caatacggaa tcgatgatgt taatgcaagc
     4321 gccgatacta ccgtcgtcag cagctgtgcc aaaatagtaa aaccgggtaa gaaaatatct
     4381 accttgcaag aagcaaagag tgcattacag attactaatg atgataaaca aaatggaaat
     4441 atcacccgtc aaaggcatgt ggtcaatgcc tatgcggtcg gcaggattgc cggtgaggaa
     4501 ggtttgttcc gcttccaatt gaatgatgat gggcagtggg gcaatcctca gttgttggtt
     4561 aaaaagatta ataaaatgga tatacggtat atttatgttt ccggctgtcc tgaagatgac
     4621 gatgccggca aagaggaaac attcagatat acggataaat tcaacagcgc ccaagatgct
     4681 gttacgcccg ccggggtgga ggttttattg agtagcggta ctgataccaa gattgccgct
     4741 tcttcagaca atcatattta tgcttaccgt atcgatgcga caatacgcgg gggaaatgta
     4801 tgcgcaaaca gaacactttg acgggaatcc cgacttctga cggacagagg gggtttgcac
     4861 tgtttatcgt gctgatggtg atgatcgtcg tggctttttt ggttgtaact gccgcgcagt
     4921 cttacaatac cgagcagcgg atcagtgcca acgaatcaga caggaaattg gctttgtctt
     4981 tggccgaggc ggctttgcgg gaaggcgaac ttcaggtttt ggatttggaa tatgatacgg
     5041 acagtaaggt tacatttagc gaaaactgtg gaaaaggtct gtgtaccgca gtgaatgtgc
     5101 ggacaaataa tgataatgaa gaggcttttg acaatatcgt ggtgcaaggc aagcccaccg
     5161 ttgaggcggt gaagcgttct tgcactgcaa aatctacagg cctgtgcatt gacaataaag
     5221 ggatggaata taagaaaggc acgagaagcg tcagcaaaat gccacgttat attatcgaat
     5281 atttgggcgt gaagaacgga gaaaatgttt atcgggttac tgccaaggct tggggtaaga
     5341 atgccaatac cgtggtcgtc cttcaatctt atgtaagcaa taatgatgag taataaaatg
     5401 gaacaaaaag ggtttacatt gattgagatg atgatagtcg tcgcgatact cggcatcatc
     5461 agcgtcattg ccataccttc ttatcaaagt tatattgaaa aaggctatca gtcccagctt
     5521 tatacggaga tggtcggtat caacaatact gtcaaacaga ttattttgaa aaatccacag
     5581 gatagtaatg atatcctcaa tataaaactg acaaggtttg tctcaggcta taagatgaat
     5641 ccgaaaattg ccgaaaaata tagtgtttcg ggggaatttg tcgatgcgcc aaaatcaagg
     5701 gcaagcaggg cgttaaaatc aagggcatac aggttggtcg gcgttccgaa ggcggggacg
     5761 ggttatacct tgtcggtatg gatgaacagc gtgggcgacg gatacaaatg ccgtgatgcc
     5821 gcttctgccc gagcctattc ggagacttta tccgcagatg ccggctgcga agctttctct
     5881 aatcgtaaaa aataaggttg ttttgccgat gccgtctgaa aatcaatgtt cagacggcat
     5941 ttttatgggt atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     6001 aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag
     6061 ctaaggcgag gcaacgctgt actggttttt gttaatccac tatacatccc gtcattccca
     6121 cgaaagtgga atctagaaat ttaatgttgc ggcactagcc aaaaaaaccg aaac
//
LOCUS       DAXIHS010000097         6163 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXIHS010000097 DAXIHS010000000
VERSION     DAXIHS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.097"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            57..1610
                     /locus_tag="ACFOF2_001801"
     CDS             57..1610
                     /locus_tag="ACFOF2_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC7108066.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVSETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1701..3728)
                     /locus_tag="ACFOF2_001802"
     CDS             complement(1701..3728)
                     /locus_tag="ACFOF2_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC7108067.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPNQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTVIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4017..6023
                     /locus_tag="ACFOF2_001803"
     CDS             4017..6023
                     /locus_tag="ACFOF2_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC7108068.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1583 a         1868 c         1433 g         1279 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgacggc aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc catctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggtcg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc catcgaaaaa atcatttgga cgggcaaaag ccggccggtc agcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgttcgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgttttccc gttttccaat gttttgaaac agaccctgct caaacgcgcg accgtgtttt
      841 tgggcatacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg cattcgcctg tttatcagcg gcggcgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctctcccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagcc aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg cttcatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag atcgaagagg aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca tttttttggc
     1681 aaacgccgga gcgaggatat ttactctgcc aattccacct gctcgtgcgc catcagttcc
     1741 tgaccgacat catccaacaa catcaaataa cgttcgtagt ttttcaaaat gtcggcaatc
     1801 agctcgtcct tgttcatata ctgcacatcg tacccgacgc gcccgtcgaa aaaataagcg
     1861 tagggtttgt aagttgtctg atgccggata tgcggcagct tgccgtcgtt aatcaactgg
     1921 tcggatacat cctgcccgac agacttaatc ccgtacataa aatcgcgcat cgtctctttc
     1981 cgaatgacga actcgattgc gggctcgtcc tgatgaaaca tcttatcgac ccggacgctc
     2041 aagccgtatt cttccgaaag ctcccgttgc aactcgtgca tagcgggcga tgcggtatgt
     2101 ttgaggaatt ttaaaatatc ctgctcctgc gtctggctca ttatctgcac cagccgttct
     2161 ttccacttgc cgcccgtcca aaatacactg gtagggttaa cccgggtctc aaaatatttc
     2221 ttatccgcac tcaatccttt ccacaggctg aaacacatta tcagcatcag cagggcaaac
     2281 ggcagggaaa caatcagggt catagactgc aggttgccga gtccgcccga gcgcatcagc
     2341 aaaacggcaa cggcagacat cagcacgccc cacataaccg cctgccaccg tggcgcgctc
     2401 aagcctttgt cccgagaggt aatattgttc aggacataaa tcccggaatc ggcagaagtt
     2461 acaaaaaaca gagaaatgac cagcaggctg acgatgctcg tcaattcggg cagggggagg
     2521 taattaaaga atttaaaaag cagcgtttcc ggagaggagg tcatcttttc gagcattccc
     2581 cccgcaaccc cgtcattcag ccaaatcacc gtattgccga agacggtaaa ccacaaaacg
     2641 ccgaacaggc cggggatgag caaaaccccg aagacaaact cgcggatggt gcgccccttt
     2701 gaaatgcgcg cgataaacaa acccacaaac ggcgcccaag aacaccacca cgcccaataa
     2761 agcaccgtcc aagattcaaa ccacggcttg tgttcccgtt cgtacgcata agttttaaaa
     2821 ctgaggcgca ccagatttcc gaggtagttc cctatgttgt cgccgaatgc cgacaacagg
     2881 taaacagtgg gacccgccgc caaaacaaaa aacagcagca aaaacgcaag gctcaggttc
     2941 aactcgctca acaccttcac gcccttcccc acgccggata ttgccgaaac gacggcgagg
     3001 gacatgacgg cggcgataat caaaacctgc acgctgaagc tgttttcggc aatccagccc
     3061 atttcctgca atccggcgcc cagttgcgaa gccccgaacc ccaatgtggt gatgatgccg
     3121 aaaaaagtag caagcaacgc cataatatca atggcatcgc cgaaccttcc ggaaattttt
     3181 tctttcaaca ggggataaaa acaagaacgc agggcaagcg gcagcttgta gcggaaaccg
     3241 aaataagcca aagccaatgc aatcgtaccg tacaccgacc aagcgtgaac gccccaatgg
     3301 aacaccgtgt gcagcaatgc ctgctgctgc ctgtgttccg gcgtgccggc cgtaatgtcc
     3361 gaaaaataat gcatcaacgg ctctgccacg ccgaaaaaca tcagacccac gcccatcccg
     3421 gccgcaaaca gcatcgccag ccacgacagg aagccgaatt ccggcacatc ttcatcccgt
     3481 ccgagcctga tgtttcccaa actgctgacc gagagtatca gcaggaaacc cagaaaaatg
     3541 gaaaacgtta aaacataaaa ccagctgaac tcggtaaaaa tgacttcttt tgcccgatcg
     3601 agccacatct gcacctgatt cggcacggtt aaaaccaata ccaccaaaac acacacaaaa
     3661 aacaaagtcg tcaaaataac catcggatta aatgacgttc ggcgttctat aaattcagac
     3721 agggacaaac cttctcactc ctttgttaaa aacagacaaa cccggtcatc gggcaaagcg
     3781 gtcaaaacct gccggtaaaa taccggcttc cggatgacga aatgcacaaa ccggccgaaa
     3841 ttgttataat cgaaaaaatt aaaaatcaat acggattatt ctatcagatt gatttatcga
     3901 tatttattat atcattaata tagtttgatt tcaaaccggc ggaaaatgcg ccgcccaccg
     3961 caggcgcgcc ctccccgcta tcggggcgtt taacgccgga ttgccgatgc ggtacaatgg
     4021 ctgatatgaa gaaaattacc cctcaaaacc tgcgccccct gctttcggaa agcttgggac
     4081 ataccgattt tgtcaacgtc ctcaacgcac tgattaaatt tttgcgccgt ggcggcaaaa
     4141 aatgtgcggg ggaacgtttc gacctgatta tcgacacatt caaacaagac agggaattac
     4201 tgtcccgctt cagccggtgt ttttacatct ggctcgcgca aatacacatt tatccggcac
     4261 tcatcaaact cggcatcttc tcgcgccaca gctttgcccg ggaaatgggc atacgcatct
     4321 acgaacgctt cagcccgtca tataaagatt ttgccaactt gggcgaagtc ttcctttatc
     4381 ttttccattc cgaaaacgac gacaaatggc tgcaaacgct caatatccgc caatggctgg
     4441 ttttatacga actcatccgg agccacgccg agccgtccaa attgcagacg gcgggcatcc
     4501 gccttgccga tgcgcgtttg cgcgccatcg aaatgctgtc tgtctggacg gcatccgaag
     4561 ccatcgaacc cgacctcatc cgcatcgccc cgcgcctgct ggaagccgat tcttccttcg
     4621 tcgccctcca acgcgaaacc gccaaactgg tcgaacacta ccgcaacggc accacgcctt
     4681 acgacaccgc ccacctcgaa gtgatgttcg accaatgttt cagccagatt gactatttgc
     4741 gccgcaaagg gacgggcgcc ggctccggtt cgtcggtcaa agtcgcccac ctgctcgaac
     4801 ggctccggca gaccgtagac cgtctaaagc tgctcaccga catccaaacc ggtgccggca
     4861 acagcaaccg cctgaccatc gcgctgatga actccctcat ctacgcggcg gtcgaacaat
     4921 acagcacccg ccacctgcgc cgcagcagca tccgtatgct cgcccgcagc attaccgaaa
     4981 acaaaagcca ccacggcgaa cactacatca cccgcaaccg caaagaatat ttcaaaatgt
     5041 tctactcggc ggcaggcggc ggcatcatca tcgccctgat ggcgctgctc aaaatccgca
     5101 tcggcacact cggcctcagc cccttcctga cttccttgtc ggctgggttc aactacggca
     5161 tcggctttat gatcatccat atgctgcact gcaccgtcgc caccaaacag cccgcgatga
     5221 ctgccgccag ctttgccgaa caggtcgatc tcaacgaagg cggcaaagcg gtggacaaca
     5281 aactcgccaa gctcctcatc gacgtatgcc gttcccaaag tgtcgccgtt ttcggcaacg
     5341 tttccatcgc catccttttg gcgtgcgcca tatcgttcgg ctatgcccat ctgtaccggc
     5401 agcccatact cgatgcccac accgccgcct accagttcaa atccatagac atcatcgctt
     5461 acccgacgct gtggtatgcc gccattgcag gtctgtggct gttctgctcc ggcatcatcg
     5521 caggtttttt cgacaaccgc gccgactacc tcaacctgcg ccaacgcctg cccttcaacc
     5581 ccttgctgcg taaaatcatg cgccccgggc cccgccgcgt cctcgccgcc tacatccaca
     5641 aacactacgg ctcgttggca ggcaacttca tcttcgggat gctcttgggt atgaccggct
     5701 atttcggaca cctcctcggg ctgccgctgg acatccgcca cgtcgccttt tcctccgcca
     5761 acctcggcta tgccgccgtc agcggcaacg tcggtttggg cacgttcgta ctcggcattt
     5821 tcagcgtcct cgccatcggc ctggtcaacc tctgcgtcag cttcagcctc gccctcttcg
     5881 tcgccctgcg ctcgcgcggc acgaaaatcg gcagcatccg caatctgatt aagagttttt
     5941 ggaatcagat taaaagcaat ccctgcatac ttttcctccc gcccgccaaa gaacagggac
     6001 atcctccttc ggacaagcct tgaccggcaa tgccgtctga agcgggattc gccccgaata
     6061 ccgccctgat gcgggaaatc cccataaaag gatgagacct ttgcaaaaat agtctgttaa
     6121 cgaaatttga cgcataaaaa tgcgccaaaa aattttcaat tgc
//
LOCUS       DAXIHS010000098         6054 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXIHS010000098 DAXIHS010000000
VERSION     DAXIHS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.098"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            121..567
                     /gene="yciA"
                     /locus_tag="ACFOF2_001804"
     CDS             121..567
                     /gene="yciA"
                     /locus_tag="ACFOF2_001804"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC7108069.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(618..1439)
                     /locus_tag="ACFOF2_001805"
     CDS             complement(618..1439)
                     /locus_tag="ACFOF2_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC7108070.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGDTEFSNVRFKGDDERAAGVYESVEFIRPEDIAETSLW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1558..2016
                     /locus_tag="ACFOF2_001806"
     CDS             1558..2016
                     /locus_tag="ACFOF2_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC7108071.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2180..3295)
                     /gene="lst"
                     /locus_tag="ACFOF2_001807"
     CDS             complement(2180..3295)
                     /gene="lst"
                     /locus_tag="ACFOF2_001807"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC7108072.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPKDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3522..3662
                     /locus_tag="ACFOF2_001808"
     CDS             3522..3662
                     /locus_tag="ACFOF2_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC7108073.1"
                     /translation="MPPHIRHSTSDTAAKSGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3729..5954
                     /locus_tag="ACFOF2_001809"
     CDS             3729..5954
                     /locus_tag="ACFOF2_001809"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC7108074.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1626 a         1769 c         1343 g         1316 t
ORIGIN      
        1 cgaatctgcc ctacgcccgc ccttgtatgt ctcaaatggg ttaaactacg cacttcccga
       61 cttcccgttt caggcagggg tgcaggtccg atttatattt caataaaaca aggaaacttt
      121 atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg aactgctcct gcgtaccgtc
      181 gctatgccgc gcgataccaa tcccaaccaa gacattttcg gcggctggat tatgtcgcag
      241 atggatttgg gcggcggcat attggcggcg gaaatcgcgc ggggacgcat cgttaccgtc
      301 gccgttcagg aaatgaactt tatccgcccg gtcaaggtcg gcaacgtcgt ctgctgctac
      361 gggcattgcg tccgcgtggg caatacttcc ctccagctta aaatcgaagt ctgggtgaaa
      421 actttgatga acgattgcgt taccgaagac cgctacctcg taaccgaggc ggtgttcact
      481 tatgttgcca tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg caaccccaaa
      541 ttgtcgggct tattgcctac tccgtaaaaa tacccgtaaa aatgccgtct gaaactgtct
      601 tcagacggca ttccgcatca tttgaaccag gacctgatgc gggaaaacag cgacatactc
      661 tgtttttcaa agtcttcccg cacttcgggc acggcttttt ttatcacttt cattcctgca
      721 aaagtctgcg ccacgggcat aatttcaatc gtgttcacat tcatatgcgc cggccggcgg
      781 tacagccaca atgaggtttc cgcaatatct tcggggcgga taaattccac actctcatac
      841 acgcccgccg ccctctcgtc atcgcctttg aagcgcacgt tggaaaactc cgtatcgccg
      901 cacaaacccg gctcgatatt ggtaacgcgg atatttttat ccgccaactc cgcgcgcaaa
      961 ttcaggctga actggcgcac aaacgccttg gtcgccccgt aaacgttgct gccggcataa
     1021 gcataattac ccgcaatcga acccaaattc atcacataac cgccgccgcg ttccaccatt
     1081 tgcggcaaaa tcttgcgcgt caggaacgtc aaacccaaaa cattggtttg aatcattgtt
     1141 tcccaatctt caaaatccgc cttgtccgcc gtgtccaaac ccaaagccag cccggcattg
     1201 ttgatgaggc agtcgatgtc ggaaaattca tcggggatgc cgtttaaggc attttccacc
     1261 gactcgcgtc gcgacacgtc catttccaaa gggtaaaaca aagcacccaa ttcatccgcc
     1321 aaaacctgaa gcctgtccgc acggcgcgcc gcaccgataa cgcggtatcc agccccgaca
     1381 aacgcgcggc acatcgcttc gccgaatcct gccgaagcac cggtaattaa aacagccatt
     1441 gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt atcataacac ccttcagacg
     1501 gcatcgcgcc gactgaaaat gacaacaacc ataccaccac gacaggacac attcaatatg
     1561 aaaacccaaa tcagccttgc tgctgccgcc atcacactgc tcctttccgc ctgcggaggc
     1621 agcggaatac cttcccaacc caaaggcgaa atttccgaaa accgcaccgc cgcgttcaaa
     1681 tctatgatgc ccgacttctc gcgtatgggc aaaatggtca aaggcgaaga gccttacgat
     1741 gtcgaaaaat tcaaacaggc tgccgcggcg tttgccgaaa gcagcaagaa accgttcaca
     1801 ctttttgagt ccgatccgca aggcaacggc cgcgccctgc cagccgtttg gtcggatggt
     1861 gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg tcgaaaaact caatgccgca
     1921 gcacaaaccg gcaaactgga tgaaatcaaa gccgcctacg gcgaaaccgg cgcaagctgc
     1981 aaatcctgcc acgacagttt ccgcgcaccc gaataacatc cgaaacagaa caatgccgtc
     2041 tgaagccttc agacggcatt ttctttgcca tttcagcaca cgcaaagcaa tcagaaatta
     2101 tcgtcaattc aaaaaatacg ccatatcctg atttttgcct tacttactcc gccattttct
     2161 attttgattt gctatgcgat taatctttat cgtcaaatgt caaaatcggg atgccggatt
     2221 gggtaaacag ggcatacacc ggcttgagcc aataatcttt cggaagggaa gccggtttca
     2281 atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa cgccgcgccg ctgaaaaagg
     2341 tataaatttc atacctcgta tgcgggtttt tcttaatctc gcgcaaaata tagtcttcga
     2401 tgacataggg cgaatttaat gtggttacgc cggaaagccc gtaggtttgg cgggggtgcg
     2461 gtgcgacata ttgtatgttg aagttttttg ccgccttttc cgaaatttcc ttcatctcct
     2521 tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt ttcatccccc gctttcagtt
     2581 cggacgcatc gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc ataatgtttt
     2641 tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt acgggttttg gcgatactcc
     2701 aatctccgat catcatccgg gcaaaattcc gcttgatcgt cccgtttaca gaaaactcat
     2761 cgcccaaata gctgctgctt tgaattaaat tgcctgtccc gtcgtcaaag gttttgattt
     2821 ccgcatccgg gtaagtgctc aaaaaggcgg caatgctgac tttttccaaa cttgccaaat
     2881 aaatccgctt gaccttcggc agcagcattg ccttcacctt cagctccgcc atcgtcggaa
     2941 tgaaattaaa cgatttgttc aaaccgtagg gcaggtggaa aaaatacgcc cgctccgcct
     3001 tatcctttat ctgattgaaa taataatcgt atttttcatt cctgttttca gacatcagca
     3061 ccacataaaa ccgctccccc ggatgctgcg ccataatcct ttccgccacc ttcatctgca
     3121 atatggtata gcagaaaatc agattgaccg gttccccctc ttcattgaag agtttgtcct
     3181 tcagcaggga aaccgcattc ctttccccct gatttacccg gtcaaatgta taaaatatcc
     3241 cgaaacaaaa aacaatcaga cacaacacgg tcaaacaagc ctttttcaag cccatatccc
     3301 taaaactcca ttccgacaaa ttaaacatac gccccgagtg tcccgggcgg aaaaatgcgg
     3361 aattttagca gatattccga cagttaccga atattatagt ggtttaaatt taaatcagga
     3421 caaggcgacg aagccgcaga cagtacaagc agtacgacaa ggcgaggcaa cgctgtactg
     3481 gtttaaattt aaaccactat aaaatcaatt tcaccgaaaa tatgccgccg cacatcaggc
     3541 acagcacctc cgacaccgcc gccaaaagcg ggaaaaccat ttcccccttc ctttcacgcc
     3601 gccgcgttca aacacgccaa gatcgaacaa agcgcgaaca aagccgccaa aatatgccat
     3661 aatagccacg cacattcccc gcgcaatgtg ttccaaaaga aaaaaccaac ctacaagaga
     3721 ataatcctat gactcaaaaa tccaccattg tttataccca taccgacgaa gcccccgcgc
     3781 tggcgaccca atcgctgctg ccgattgtgc aggcttttgc ccgccacgcc gatattgatg
     3841 tcaaaactgc cgacatttcc ctgtccggcc gtattttggc ggcattcccc gaatatctga
     3901 ccgaggcgca gcgcgtaccc gacgcgcttg ccgaattggg cgaactggta aaacaacccg
     3961 atgcaaacgt aatcaaactg ccgaacatca gcgcatccgt acctcaactg accgccgcga
     4021 ttaaagaatt gcagtctaaa ggctttgccg ttcctgacta tcctgccgac cctcaaaccg
     4081 atgaagaaaa agccgtacgc gaacgctacg accgcatcaa aggcagcgcg gtaaaccctg
     4141 tcctgcgtga aggcaactcc gaccgtcgcg cccccaaagc ggtgaaaaac tttgccaaaa
     4201 aaaatccgca cagcatgggc gcatggacaa aagactccaa aacccacgtt gccaccatgc
     4261 aaagcggcga ctttttccat aacgaacaat ccgttaccgt acctgaagcg acttccgtat
     4321 ccatcgtgtt caccgacaaa caaggcaata aaaaagagct gcgcgcgcct gttgccctga
     4381 aagccggcga aatcatcgac gcaaccgtga tgagcaaaaa agccctgctc gccttccttg
     4441 ccgaacaagt gaaagacgcg aaggcaaaag gcgtattgtt ctcactgcat atgaaagcca
     4501 cgatgatgaa agtgtccgac ccgattatct tcggacacgc cgtcaaagtg ttcttcgcgc
     4561 ctgtatttga aaaattcggc ggcaaactgg ctgccgcagg tgtcaacgtc aacaacggct
     4621 tcggcaacct gctggccaat ctggacaaac tggatgcgga cacccgcgcc gccgtcgaag
     4681 ctgaaatcgc cgccgtttac gccgccaacc ctgatttggc gatggttgat tccgataaag
     4741 gcattaccaa cctgcacgtt cccagcgatg tcatcgttga tgcttctatg cctgcgatga
     4801 ttcgtaactc cggccgtatg tgggataaag acggcaaagc gcaagacacc aaagccgtca
     4861 ttccggacag cagctatgcc ggtgtttacc aagcaaccat cgacttctgc cgcgaacacg
     4921 gcgcattcga tccgacaaca atgggtactg tgcccaatgt cggtttgatg gcgcaagcgg
     4981 cggaagaata cggctcacac aacaaaacct ttgaaatcga agccgacggc caagtccaag
     5041 tcattgatgc ggcaggaaaa gtcctgatgc agcatgacgt tgaagcaggc gacatttggc
     5101 gtatgtgcca aaccaaagac gctccggtta aagactgggt gcaacttgcc gtcaaccgcg
     5161 cccgtctgag caacacgcct gccgtgttct ggctcgacga aaaccgtccg cacgacaaga
     5221 gcctgctcgc caaggttaaa gcctaccttg ccgaactgga taccaatggc ctcgacatcc
     5281 gcgtcctcgc tcctgaagaa gccgccaagt tcagcttggg tcgtctgaaa aacggcgaag
     5341 atactatctc cgtaaccggt aacgtcctgc gcgactacct gaccgacttg ttcccgattt
     5401 tggaactcgg cacaagcgcg aaaatgctgt ctatcgttcc attgatgaac ggcggcggta
     5461 tgtttgaaac cggcgcgggc ggttctgcac cgaaacatgt tcaacaattc ctcgaagaaa
     5521 accacctgcg ttgggactct ttgggcgaat ttctcgcact cgccgtatcg tttgaacatc
     5581 tggcgcaaaa aaccggcaac accaaagccc aagtcctcgc cgacactttg gatgcagcca
     5641 ccgaaaaact gctgttgaac gacaaatcgc ctaaacgtaa agccggcgaa ctcgacaacc
     5701 gcggcagcca tttctacctc accctctact gggcgcaaga attggcggcg caagacaaag
     5761 atgccgaact gaaagccgca tttacgccat tggcagccgc tttgaccgcc gacgaagcga
     5821 aaatcgttgc cgagctttcc gccgtacaag gcaaagcggc cgacatcggc ggctactacg
     5881 ccgccaatcc tgaaaaagcc gcacaagtga tgcgtccgag cgtaaccttt aatcaggcac
     5941 taaacgcctt ataagcacaa aggtaaaaat gccgtctgaa cattcgtcgt tcagacggca
     6001 ttagttatcc ctatctgcct gattatgatt aaacgccgca cccattggat ataa
//
LOCUS       DAXIHS010000099         6021 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXIHS010000099 DAXIHS010000000
VERSION     DAXIHS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.099"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..207
                     /locus_tag="ACFOF2_001810"
     CDS             <1..207
                     /locus_tag="ACFOF2_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108075.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMG
                     IKSISDTSRAMPSENQSPLSDGIV"
     gene            284..1231
                     /locus_tag="ACFOF2_001811"
     CDS             284..1231
                     /locus_tag="ACFOF2_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC7108076.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="ACFOF2_001812"
     CDS             complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="ACFOF2_001812"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC7108077.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2507..3310
                     /locus_tag="ACFOF2_001813"
     CDS             2507..3310
                     /locus_tag="ACFOF2_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC7108078.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3368..5587)
                     /locus_tag="ACFOF2_001814"
     CDS             complement(3368..5587)
                     /locus_tag="ACFOF2_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC7108079.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVFPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQTGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1492 a         1892 c         1433 g         1204 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgctata aacagatggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggcc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatggcctat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggtg aaatgctgga aatccaagcg gccggtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacga aaccggcggt ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cagggacgat tgtccggctg ctccgattcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctcactcc cttacataaa ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc
     1441 gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg ctcaaagacg aatccaaacc
     1501 ggcttcaaga cggaggacag acaacaaatg atatatgtcg tccggaatcg gtgcttcaaa
     1561 cgaaacggtt tcgccggttt tcggatgagt gaaacttaag cggtaggcgt gcaatgcctg
     1621 acgcgcaccc aaacttttaa cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt
     1681 gccgtaaacc gggtcggcgg caagcggatg gttggcctca cgcatatgga cgcggatttg
     1741 gtgcgtcctg cctgtttcga gcgagcattc gatatagctg tgcgccagat agcgttccaa
     1801 cactttgacg tgggtaacgg ctggtttgcc gccgaatttg acgactgcca ttttcaggcg
     1861 gttgtgcgga tcgcgtccga tttgggtttc gattttaccg tcaaagggaa cgatgccgtt
     1921 ggcgacggcg cggtaaatgc gtttgaccgt gcgctcttga agctgttgca cgagggaatt
     1981 ttgcgccggc agggttttgg caaccaccat cagcccgctg gtttctttgt ccaaacggtg
     2041 tacgatgcct gcacgaggta cttggctcaa ttcgggacag tgcgccaaga ggccgttgag
     2101 cagcgttccc gtccagttgc cggccgccgg atgcaccacc agtccggccg gtttgttgac
     2161 gacgatgacg gtatcgtctt cgtaaacaat atccaaagcc ataggctctg gaacaaacgc
     2221 cagattttcc tcactcggac ggacggttac acaaatttgc tcgccgccta tcattttgtc
     2281 tttgggttgc gaaggtttat cgtttacaat aaccgcgcct tctttaatcc atgatgtcag
     2341 gcggctgcgc gagtagtcgg gcagaagttt cgccaacacc gcatccaacc gcccgccggc
     2401 aagctcgagc ggaacggtca gaccgatacg gttttcagtt ccggaagctg acgcaaagtc
     2461 tgaatcgtcg ctataatcgg attcattatc aaaggaagta ttctgcatga aaaaaattct
     2521 tttaacggtt tcattaggtt tggcactgag tgcctgtgcc actcaaggta cggtcgataa
     2581 agatgcccag attacccaag attggagtgt ggagaagctc tatgccgaag cccaggacga
     2641 attgaacagc agcaattata cgcgggctgt caagttatac gaaatcttgg aatcgcgctt
     2701 ccccaccagc cgccatgccc ggcagtcgca actggatacc gcatacgcct attataaaga
     2761 cgatgaaaaa gacaaagctc tggcggcaat cgaacgcttc cgccgccttc acccgcaaca
     2821 cccgaatatg gattacgcgc tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc
     2881 cttcttgaac aaactggcct cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga
     2941 agcgtaccag gcgtttgcgg aactcgtcca acgcttcccc aacagcaaat acgccgccga
     3001 tgcgaccgca cgcatggtca aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc
     3061 gcgctactac atgaaacgcg gtgcatacat cgccgccgcc aaccgcgccc aaaaaatcat
     3121 cggcagctac cagaatacgc gttatgtcga agaatcgctc gccatattgg aacttgccta
     3181 ccaaaaactc ggcaaaccac agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt
     3241 cccgaaaagc ccgtttttga cgcacgcttg gcagcccgac gatatgccct ggtggcgtta
     3301 ctggcattaa gcccatcttc gtaatgccgt ctgaagccgt ttcagacggc attttatgtt
     3361 tcaaacctca ttcaaacggt tttttctgcc aatagaattt ataccctctc aaacgttgag
     3421 ccttcacgcc gccgcgcccc aagccgaatt gcagcgcgcc tgacaaatcg gtacgcagca
     3481 gtttgatgcc gtgtgcgcgg acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt
     3541 tggcataacc gcttgaagca acagcatatt cgggcgagac ggcattgagg aacacgcccg
     3601 acgaggacgt attgctgccg tgatgtccca acaccaaaac ctggctgtac aggttgcctc
     3661 catacttgcc gaccaggctt tcctcaccct tcgtatccaa atcgcccgtt accagcagtg
     3721 ccgcaccgcc cgccacaaca cgcaaaacac aacttttccc attatcatcg atgtttttgc
     3781 gttcagacgg catcaaaaac tcgaaatcca cgccgtccca ttgccaacgc tgttccgcac
     3841 aatgccgcgc cccctcataa aattccggct gtccggcata aatcccgccg ttcggtatct
     3901 tgccgactgc ctgaaaaccg ccgtcgtggt cgctgtcgtg atgcgacaaa accaaataat
     3961 ccaaatacct gattcccgac gcattcaggc tcggcacaat ccccgtctgc gccgccgctg
     4021 ccgttccggt gtcaaacaaa agatgatgat ttgccgtctg aaccgacacc gacaaaccct
     4081 gccccgcatc ccaaaccgta accgcaacct cattttccgg cacgccgggt gaacggtaaa
     4141 acacaaaccc tgccaacagc aacaccgccc acggacgcaa acccaagccg cgcggcagca
     4201 gcaacagcaa agcggcacac accgccaaca ccaacagcgg caaaggtgcg gaggcaacgg
     4261 caaactcggg cgacacatcg gcaagccaca ccaaaaaccg caaagtgtat tccgccaaaa
     4321 acgcccccgc ctgctgaagc ggcgcaaacg gaaaaaccga acccagcaac gccagcggcg
     4381 tcaataccca agaaaaccac ggaattgcca ccgcattgac caaagggctg attaaaggca
     4441 gcgaagcaaa cagataaccg agcaaaacca gcgacaacac cgaagccgcc cactgcccgc
     4501 gcaaagcagt ttgccgtttt ccctcgtgca aacgccccga gcacgcccat atcagggccg
     4561 ccaccaaacc gaaagacagc caagtcccca cacccaagac cgccgacggg tcgaacagca
     4621 gcaccgccgc caacgcctgc caccacgtcg cccacgccga caatcttccc ctgcgccaag
     4681 cccacgcaaa cgccgccaac atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa
     4741 gcagcgcgta aaacagcgcg cctgcacacc ccgccgccaa aacccacgcg cgcggccggg
     4801 caggaaggcg cggggaacag gcaagcagcc gcttcgccag ccacgcaaac attaccgcca
     4861 ccatcgtaac gtgcaaaccc gaaatactga ccaaatgcgt cagccccaac ggccggaacg
     4921 cctgccacaa ttcggggcgc aatgccgact gttcgcccac gctcaacgcg cgcatcagcc
     4981 cgatgccgtc tgaaagcccg ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc
     5041 tgcgccaaac cgcaatcccc caaccgcttc cgccatgcag caaaaccctg tccgcaccga
     5101 ccgtccccgc accgcctatt ccgttggata atgcccacgc ctcacggttc aaaccgcgca
     5161 ggttcaactc cccgacgaca gggtgcacac gtgccgttat ccgccatctg ctcccgaccg
     5221 cccattcgcg ccgtttgtag tccgacagca acaaatcaaa cgtccgacca ccgctgtcca
     5281 cagcctttgc cgcaaactgc acacgccgcc cgtccgacct cggcatatcc gccacttcca
     5341 ccgtcaacgg cacacctgaa accgcctccg cccgccattg cgaagacagt gccgcttccg
     5401 ttctgaatac gccgtaagcc gcgcccgcca acacgcacag catcagaccg gcaaacgcaa
     5461 aacgccttgc aaacacagca aaaaccgcaa aagccgccag ccaaaacagc caatgcggca
     5521 cgaccggcag cgcaaacgaa gccgccgcgc cgacaaccca acacggcaaa aaccaacgac
     5581 ggaacacgtc tccccacccc agatatttca aaaataaacg gataataaag caaatccgaa
     5641 aaaccgtcat aacaagattt gaaaatgata tagtggttta aatttaaatc aggacaaggc
     5701 gacgaagccg cagacagtac aagcagtacg acaaggcgag gcaacgctgt actggttttt
     5761 gttaaaccac tataagaatg caaacgaaaa gcctcagtat tatccgagac aacggcaaac
     5821 tttccatcgt cattcccacg aacccacatc ccgttattac cacgaaagcg ggaatctagg
     5881 actcagggtt aagaaaacct acatcccgtc attccctcaa aaacagaaaa ccaaaatcag
     5941 aaacctaaaa tcccgtcatt ccctcaaaaa cagaaaacca aaatcagaaa cctaaaatcc
     6001 cgtcattccc acgaaagtgg g
//
LOCUS       DAXIHS010000100         5977 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXIHS010000100 DAXIHS010000000
VERSION     DAXIHS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.100"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            170..1144
                     /locus_tag="ACFOF2_001815"
     CDS             170..1144
                     /locus_tag="ACFOF2_001815"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC7108080.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALCGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1303..1521)
                     /locus_tag="ACFOF2_001816"
     CDS             complement(1303..1521)
                     /locus_tag="ACFOF2_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC7108081.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            1865..2127
                     /locus_tag="ACFOF2_001817"
                     /pseudo
     CDS             1865..2127
                     /locus_tag="ACFOF2_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            2295..4070
                     /gene="ggt"
                     /locus_tag="ACFOF2_001818"
     CDS             2295..4070
                     /gene="ggt"
                     /locus_tag="ACFOF2_001818"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC7108082.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            4133..4396
                     /locus_tag="ACFOF2_001819"
     CDS             4133..4396
                     /locus_tag="ACFOF2_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108083.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            4551..5801
                     /gene="glyA"
                     /locus_tag="ACFOF2_001820"
     CDS             4551..5801
                     /gene="glyA"
                     /locus_tag="ACFOF2_001820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC7108084.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSAIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYTVYGA"
     gene            complement(5761..>5977)
                     /locus_tag="ACFOF2_001821"
     CDS             complement(5761..>5977)
                     /locus_tag="ACFOF2_001821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108085.1"
                     /translation="VFGNHGLGGLLEEGAHEKSPKCLGGNLGDFGEFCKGLRLKHCEI
                     SGGIGLLGGIFLKRLTRRKRCICRRGR"
BASE COUNT         1657 a         1494 c         1370 g         1456 t
ORIGIN      
        1 ccactataag aataaatatc ccctcatccg cctgccgcgt tccgacttgc cccgcgccgt
       61 ttttttccac tacaatccca cgcgatatct ggcggaacgc accaaaggaa cgccccgcag
      121 caatctgccg cctttcagac ggcacaaaca tttccgaaac gagcaaacta tggacacact
      181 gacccgattc ctccccgaac acctgcaaca aaaccagctg cccgaagcac tctgcggcgt
      241 tttactgtcc gttgtttccg cctgcaccga aatcaacgcc aaagtccgcc tcggcgcact
      301 ggccggcgta ttgggtatgg cgggcacggg caatattcag ggcgaagacc aaaaaaaact
      361 ggatgttatc gccaacaaca tcatgattga cacactcaaa gccaaccctg ccgttgccgg
      421 tttggcaagc gaggaagaag actctttcgt aagtgccggt gaaaacggac gctatctcgt
      481 cctattcgat cctttagacg gatcgtccaa tattgatgtc aacatttccg tcggtacgat
      541 tttctccatc cttgaaaaac ccgaaggcgc attggcaacc gaatcattcc ttcaaacggg
      601 cagacagcag cttgccgctg gttacgttct ttacgggcct caaacccagc tcgtattcac
      661 attcggacac ggcgtatata tgttcacgct caatgccgaa aacgaatttg tgctgacaaa
      721 agaaaaaccg aaagtacccg aaagtaccaa agaatttgcc atcaatatgt ccaaccgccg
      781 ccactggctg ccccccgtcc aacaatacat cgacgagctc ttggcgggcg aaaccggcac
      841 gcgcggcaaa aactacaata tgcgctgggt agccagcatg gttgccgaaa tccaccgcat
      901 cctgatgcgc ggcggcgtgt tcatgtatcc gcaagacaaa cgcgaccccg ccaagcccgg
      961 caaactgcgc ctgatgtacg aagccaaccc tatgagcctg atactcgaac aggcgggcgc
     1021 atcggcaagc aacgcatatc aagccatgct cgacatacag cccgaaaacc tgcaccaacg
     1081 cgtcgccgtc attatgggca gcagcgaaga agtggattac ctcaaccgcc tgcattcaaa
     1141 ataaaaggca gcctgaccga taaagaaagg cggcagcgcg catccgacgc tgccgccgat
     1201 atttcgttta tagccttaaa aagatagaaa accacctttc cgaacggcaa aaatgccgtc
     1261 tgaaaagttt tcagacggca ttttaaaaat taaagcccga atttatttgc ccgctgcttt
     1321 ttgcaggatg gcgtacaact cgtctttcag tttcaatttg gcttttttca gctcatcgat
     1381 ggtttccgca ccgctggtaa ccggattgtt gaccagaccg gtaattttat cgtccagctc
     1441 gttgtgttcg tcgaacagac gggcgaagcg ggaattttcc tgtttcaatt tggaaatcaa
     1501 atcacggtat tctgggaaca tatcgctttc ctctcttggt ttacggttaa aaagtcaaat
     1561 tgaatcttta cattaattat aacaaagata atatttttat ccagtgtttt atcggatacg
     1621 gcaaaatttt cttttaacac cgccgtccgg ttgataaaaa cgttgacaat atcacgcttt
     1681 acgcataaat tttcagcctt tcctactgta tagtggatta acaaaaatca gggcaaggcg
     1741 acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta
     1801 gagaatcgtt ctctttgagc taaggcgaga caacgccgta ctggtttttg ttaatccact
     1861 atatatgaaa ggcacgcccc agtttcccca atgtggattt tcagcgcgtg cagtagaggt
     1921 gctgacccaa ttggcagacc ctttgctttt gttaatatcc tagaaaaccc agaaattcgc
     1981 agtactctac cactcatcgc caatcggcca acctttccac agctttgggt taatggtgag
     2041 ttaatcggtg gtagtgatat tatcctacaa atgtaccaat caggtgagct aaaaccactc
     2101 attgaagagc atagcccaaa ggtctgatta cttaatatct taaaataaat atcaaaaaag
     2161 actgctgatg acattagcgg tctttttttg atggtcaaga ttgatagttt ggattgaatt
     2221 atttagccat attaaataca ataatcagca tgccttgtat gaatcatcaa tcaaactcag
     2281 gcgaaggagt gcttgtggct aaaacatatt tattgactgc attgataatg tctatgacaa
     2341 tctctggatg tcaagtcatc catgccaatc aaggtaaggt taatactcat tctgctgtca
     2401 tcacaggtgc agacgctcac acgcctgaac atgcaacggg actgatcgaa caaaagcagg
     2461 tgattgcaag tgattttatg gtagcgtcag ccaatccatt agcaacacaa gctggctatg
     2521 atatcttaaa gcaaggcggt agcgctgcag atgcgatggt ggcggtgcag acgacactaa
     2581 gcttggtaga gccacagtcg tcaggcttgg gcggtggtgc atttgtgttg tattgggata
     2641 ataccgccaa aacattgacc acatttgatg ggcgtgagac ggcaccgatg cgtgcgacgc
     2701 cggaattatt tttggataaa gatggtcaac cattgaaatt tatggaagcg gtggtcggtg
     2761 gtcgctcggt gggtacgcct gctatcccta aactgatgga gacaatacat cagcgatacg
     2821 gtgtattgcc ttggggaaaa ttatttgata caccgatccg cttggcaaaa caaggctttg
     2881 aggtgtcgcc aaggcttgcc atctcggttg agcaaaatca gcagcatttg gcacgctatc
     2941 caaaaacagc cgcttatttt ttgccgaatg gtgtgccgct acaagcaggc agcttgctga
     3001 aaaatttaga atttgctgac agtgttcagg cgttagcagc tcaaggtgca aaagctctgc
     3061 atactggtaa atatgcccaa aatatcgttt cagttgtcca aaatgctaag gataaccccg
     3121 gtcaattatc cttacaagat ttatctgatt atcaagtggt ggaacgcccg cctgtttgtg
     3181 tgacttatcg tatttatgaa gtatgcggta tgggtgcacc aagctcaggt gggattgctg
     3241 tgggtcaaat tttggggatt ttaaatgaat tttcacccaa tcaggtgggg tatgatgctg
     3301 aaggtttacg tctcttgggc gacgcttcta ggcttgcgtt tgcggatcgt gatgtatatt
     3361 tgggcgaccc tgattttgta ccagtaccca ttcgccagtt gatttctaaa gactacctaa
     3421 aacatcgcag ccagctgctt gagcagtcgg ataaggcatt gcctagcgta tctgcaggcg
     3481 attttattca tgagtgggtg tcctcacaag cgattgagtt accttctacc agtcatatat
     3541 caattgtgga taaggcgggc aatgtgctat caatgacgac ttccattgaa aatgcgtttg
     3601 gctcaacttt gatggcaaat ggctacctgc tgaataatga gctgacagac tttagttttg
     3661 agccaataaa gcagggcaaa caggtcgcca atcgtgtcga gcccggcaag agaccacgct
     3721 cctcaatggc accaaccatc gtatttaaag caggcaagcc ttatatggca atcggctccc
     3781 caggtggtag ccgtatcatt ggctatgtcg ctaagacgat tgtggcacat agtgactgga
     3841 atatggatat ccaagatgcc atcagtgcac ccaatttatt aaatcgcttt ggtaactatg
     3901 aactagaaac gggtacaacc gccctccagt ggcaacaagc attaaatgat ttgggctata
     3961 agactgacgt gcgtgagcta aattcaggtg ttcaagccat tattatcgag ccaagcaggc
     4021 tggttggtgg tgccgatcca cgccgagaag gtcgagtgat gggtgattag aggtgaagct
     4081 tgtattttgt gaatttgacc gcattaagtt aatattaaaa tagggggaaa ttgtgagtaa
     4141 tcaaacaaaa atacaagcca tcaagcaagc ttctgagcag atcttcgcaa tttgtgagac
     4201 gccaaacaca gcattacaag ccattcatct gattttaaga catggtgggg caggagagtt
     4261 gtcttggcaa gtggtgtatc agcgggttat ggctgataaa gatgtggttg gtgctggtta
     4321 tttgattgat tttgcacaaa ctgccgaaaa tttaccattt gatgtattgc ctttgggtag
     4381 ccttgtacta acataaaggt aatgagacgc taaaaaccag catgttaaat aaattacctg
     4441 ataatgccaa agaaaattta cgcattatgg actatctgcc ttaggttttg aagtattttt
     4501 ttatacaaaa aacactcatg ataccctgat tacacaaagg agccgagaac atgttttcaa
     4561 aaagcgttac cctcgcacaa tatgaccccg atttggcagc agccattgcc caagaagacc
     4621 aacgccagca agaccacgtc gagctgattg cctctgaaaa ctacgtcagc tgcgccgtaa
     4681 tggaagcgca aggttcccag ctgaccaaca aatacgccga aggctacccc ggcaaacgct
     4741 actacggcgg ctgcgaatac gtcgatattg tcgaacaatt ggcaatcgac cgcgtcaaaa
     4801 aactgttcgg cgcgcaatac gccaacgttc aaccgcactc cggctcgcaa gccaaccaag
     4861 ccgtgtacgc ttccgtttta aaaccgggcg acaccatttt gggtatgtct ttggcgcacg
     4921 gcggccacct gacccacggc gcgagcgtta atatttccgg caaactctac aatgccgtta
     4981 cttatggttt ggatgaaaac gaagtgctcg attatgccga agtggaacgc ttggctctcg
     5041 aacacaaacc taaaatgatt gtggccggtg cgtctgccta cgcgttgcaa atcgactggg
     5101 caaaattccg cgagatcgcc gataaagtcg gcgcatacct ctttgtcgac atggcgcact
     5161 atgccggact gattgccggt ggcgaatatc ccaaccccgt gccattctgc gacttcgtga
     5221 ccaccaccac ccacaaaacc ctacgcggcc ctcgcggcgg cgtgattttg tgccgcgaca
     5281 atacccacga aaaagcgtta aactccgcca tcttcccaag cctacaaggc ggtccactga
     5341 tgcacgtcat cgccgccaaa gccgtagcgt ttaaagaagc gttgcaaccc gagttcaaac
     5401 aatacgcaaa acaagtgaaa atcaatgctg ccgccatggc agaagagttg gttaaacgcg
     5461 gtttgcgcat cgtttccggc cgcaccgaaa gccacgtttt cctcgtcgac ctgcaaccga
     5521 tgaaaatcac cggtaaagcc gccgaagccg ctttgggcaa agcgcacatc accgtcaaca
     5581 aaaacgccat cccgaacgat ccggaaaaac ccttcgttac ctccggcatc cgcatcggct
     5641 ccgctgccat gaccacacgc ggttttaacg aagccgacgc gcgcgtattg gcgaacttgg
     5701 ttgccgacgt attgtctaac cccgaagacg aagcgaatct ggaaaacgtc cgcaaacaga
     5761 ttaccgccct ctgcgacaaa tacaccgttt acggcgcgta agccttttta aaaatattcc
     5821 gccaagcaat ccaatgccgc ctgaaatctc acaatgtttc aggcggagac ctttgcaaaa
     5881 ttccccaaaa tcccctaaat tcccaccaag acatttaggg gatttctcat gagcaccttc
     5941 ttccagcaaa ccgcccaagc catgattgcc aaacaca
//
LOCUS       DAXIHS010000101         5796 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXIHS010000101 DAXIHS010000000
VERSION     DAXIHS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.101"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(389..1189)
                     /locus_tag="ACFOF2_001822"
     CDS             complement(389..1189)
                     /locus_tag="ACFOF2_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC7108086.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTMAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1325..1828)
                     /locus_tag="ACFOF2_001823"
     CDS             complement(1325..1828)
                     /locus_tag="ACFOF2_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108087.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(2165..2806)
                     /locus_tag="ACFOF2_001824"
     CDS             complement(2165..2806)
                     /locus_tag="ACFOF2_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC7108088.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            3134..5347
                     /locus_tag="ACFOF2_001825"
     CDS             3134..5347
                     /locus_tag="ACFOF2_001825"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC7108089.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LTFDGLFDIRAVRILVDTIPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
     gene            5640..>5796
                     /locus_tag="ACFOF2_001826"
     CDS             5640..>5796
                     /locus_tag="ACFOF2_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC7108090.1"
                     /translation="MSQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVKSDRQK
                     PAQYIGQH"
BASE COUNT         1749 a         1481 c         1173 g         1393 t
ORIGIN      
        1 ctcttttggg cttattcaaa ttagggacac gccctagaac caagttgcat gataatacct
       61 ttcattaact gaaacactga ttaagaaact ccagtctgtc taatgatgag gttttcacat
      121 cgccaaaact tgccaatcaa atgctggatt tattgccgtc tgagatttgg tcaaatccaa
      181 aggcgacatt cttagaccct gtgtgtaaat caggggtatt tttgcgtgaa atcgtcaaac
      241 gcttggatga aggcttgacc aatcaaatac cagataaaca aactcgcatt aaccacattt
      301 taaaaaatca agtttttgga agtactgcca cgtatgtagg tagctttgac cgatatttgc
      361 ataaaaactc ctttgctggt gaaaggaatt attttgccaa ttttaaaata tttctggcac
      421 caaatagtac aatgacaaag acaatcatgc caatgattaa atcaggatag ctagaatgag
      481 tcaataacgt caatgctccc gccgctatca caccgatatt gatgataatg tcattggatg
      541 taaaaatcat gctggctttg atatggattt ctttattttg atttttgctc agtagatata
      601 agcacagcca gtttgcaatc aatgccaaaa atgccgtgcc aatcatcagt tgataattgg
      661 gcagctgctc agcaccgata aaacgcctaa tcacttctat caccccaaat aacgccaata
      721 ttatctgcgt tatccccgcc aaaaatgcca cacgtttttt atacgccatc gtcataccaa
      781 tggctgatag cgccaatata tagacaaagc tgtccgccag catatctaga ctatcagcaa
      841 tcagccccat agaattagca aaaataccaa ccgaacactc tatgataaaa aacacaaagt
      901 taatcatgag cacttgatat aataatcttt tttctaagtg ctcatcaggc ttgttaaaca
      961 ctatcttatc aacaatcact tcggtggaaa tgatatgact atcaaaatta agcggttcaa
     1021 gtacttgtaa aatcgttgta tcttgattat cgtgatagac ggttaagcac cgcccagcaa
     1081 tatcaaactg taattcataa atatcagaca catcttttaa acgcatgcga atgagctgtt
     1141 cttcggacgg gcagtccatt ttggtaatgt taaaaatggt ctttttcatc tatttagttc
     1201 cttgttttga tcaggttggc tcaaataaat ctgtgtttat attgctgctt ggtaattttt
     1261 ggatggtttg agtaaattga ttaggttaaa atttaccttt ggaagtaccg ccacgcataa
     1321 tagtttagat atgtttataa tctctggata aaaaaacgta ataagtgctt actggataac
     1381 aaagtccaaa ccaatagcag gcaaaataag gcatccaccc cccttcttca ttaaggatat
     1441 atattgagaa acaaatcgca actaaacaga aaaaacttgg gagataaagc catttcattc
     1501 ccctattcaa gaatctagcc aagataggta ttttgtattc tacaaaaaag aaaggcattt
     1561 ccaagggaaa catgtcagat aaaaactttt gtttattttt tactatagat agaaccttgc
     1621 ttctcaagag aaagccatta ataataccga tgacagctat taatatatag agaatagtat
     1681 aagtatgaat aatcttcatt agacaaaaag aagaaatggc agataaatta catacgatat
     1741 attggaatat aaaatattta cggtctaaac cttgttcagt tgcaattttt ttaaaattgc
     1801 cttgcataaa aaaatcaaag gcgtccatta aactatcttt cacattagaa atttaaaagc
     1861 taaataatac gacaaacaat gtgaagtact attcatggtt tatttaaaaa ataatactat
     1921 tctgaacatt atttagatac agaaattaac aaattagaac taaacaagct tttaaatact
     1981 ttaattttat tggaaagcta taaaaggaac tataacttta cacactagtc acttcttttt
     2041 aagaggcaaa agggattggg aaggtcgtct tggagataag cactggtatt tcggccaatg
     2101 gtaaatagag tttacctcaa atagggtaga acctccttca tctgtcagtt aataacagcc
     2161 acttttacaa tgccctgtca aaataaagcg gcacgcccga tttttcactc atcgtcatca
     2221 aataacccat caccttttgg ggccattcga tgccgcgcac cacggtcaga ttcctcaaaa
     2281 cggggaaaac caaaatatcc tccataccga ttccgccgtt gatgccgtct gaagcaccgt
     2341 ccatcaaatt ttccaactct tgcaaatctg cgtttatgcg ttcgagatat tgggcggttt
     2401 tattcaaatt ggcggaaaag ctgccgatgc ttttctcttt tttgtctgta aaatatttta
     2461 ccgcttccgg cgttgcaaat tcgggcagcc cgattttgat cacgcgcggc tgcaccagtt
     2521 tgtcgttgta tccgcccacc ttgtccagcc acgcccgtat ctcggggcgg acttcgtctt
     2581 tcagacggtc ttcgcggtcg aaatgccgca caatgtccaa actctcgccc ataaacgaac
     2641 cgtcttcttt ttgcaggacg ggcacttgtt tcgcaccgat cataccgatc ggcgttgcct
     2701 cgtcgtcgtt tgccagcacg gcttcttcaa cgtccgcgcc aaacagcccg gcagccatcc
     2761 gcgcacgcac acaaaacggg caatggtcgt aaatatacag tttcatcaaa atattcctcg
     2821 tcaacctgtc ggtaccggct accttaacac cccgcgccac ccgaaacaag tttatcttcc
     2881 cgcctatgca ccgtaaataa ataagctgag acctttgcaa aaatagtctg ttaacgaaat
     2941 ttgacgcata aaaatgcgcc aaaaaatttt caattgccta aaaccttcct aatattgagc
     3001 aaaaagtagg aaaaatcaga aaagttttgc attttgaaaa tgagattgag cataaaattt
     3061 tagtaaccta tgttattgca aaggtctcaa gctgttacaa taaactcgtt tttatcggaa
     3121 cgaaagaccc atcatgaccg ccatcagccc gattcaagac acgcaaagcg cgactctgca
     3181 agaattgcgc gaatggttcg acagctactg cgccgcgctg ccggacaacg ataaaaaact
     3241 tgtcttagcc gcccgttcgc tggcggaagc acattacccc gccgatgccg tcacgccgta
     3301 tggcgagccg ttgtccgacc acttcctcgg cgcggcacaa atggtcgacg aactcgacct
     3361 gctgcccgat gccgtcgccg ccaccctgct tgccgacatc ggacgctacg tccccgattg
     3421 gaatctattg gtttccgaac gctgcaacag caccgtcgcc gagctggtca aaggtgtgga
     3481 cgaagtgcag aaactcaccc acttcgcccg ggtggacagc ctcgccacgc cggaagaacg
     3541 cgcccagcaa gcggaaacca tgcggaaaat gctgctggcg atggttaccg acatccgcgt
     3601 cgtgttaatc aaactggcga tgcgtacgcg caccctgcaa tttttaagca acgcccccga
     3661 cagccccgaa aaacgcgccg ttgccaaaga aaccctcgac atcttcgccc cgctcgccaa
     3721 ccgtttgggc gtgtggcagc tcaaatggca gctcgaagat ttgggcttcc gccatcaaga
     3781 acccgaaaaa taccgcgaaa ttgccctgct tttggacgaa aaacgcaccg aacgcctcga
     3841 atacatcgaa aacttcctca atatcctgcg tacggaactg aaaaaataca atattcattt
     3901 tgaagttgcc ggaagaccga agcacatcta ctccatttac aaaaaaatgg tgaagaaaaa
     3961 gctcactttc gacggcttgt tcgacatccg cgccgtacgg attttggtgg acaccattcc
     4021 cgagtgttat accacgctgg gcattgtcca cagcctctgg cagccgattc cgggcgagtt
     4081 cgacgactac atcgccaacc cgaaaggcaa cggctataaa agtttgcaca ccgttatcgt
     4141 cggcccggaa gacaaaggcg tggaagtgca aatccgcacc ttcgatatgc accaattcaa
     4201 cgaatttggt gttgccgccc actggcgtta taaagagggc ggcaaaggcg attccgccta
     4261 cgaacagaaa atcgcttggt tgcgccaact cttggactgg cgcgaaaaca tggcggaaag
     4321 cggcaaggaa gacctcgccg ccgccttcaa aaccgagctt ttcaacgaca cgatttatgt
     4381 cctgaccccg cacggcaaag tcctctccct gcccacgggc gcgaccccca tcgacttcgc
     4441 ctacgccctg cacagcagca tcggcgaccg ttgccgcggt gcgaaagtcg aagggcagat
     4501 tgtgccgctg tccaccccgc tcgaaaacgg acagcgcgtc gaaatcatta ccgccaaaga
     4561 agggcatcct tccgtcaact ggctttacga aggctgggtc aaatccaaca aggcaatcgg
     4621 caaaatccgc gcctacatcc gccagcaaaa cgccgacacc gtgcgcgaag aaggccgcgt
     4681 ccaactcgac aaacagcttg ccaaactcac gcccaaaccc aacctgcaag agcttgccga
     4741 aaatctcggc tacaaaaagc cagaagacct ctacaccgcc gtcggacaag gcgaaatttc
     4801 caaccgcgcc atccaaaaag cctgcggcac gctgaacgaa ccgccgcccg tacccgtcag
     4861 cgaaaccacc atcgtcaaac agtccaaaat caaaaaaggc ggcaaaaacg gcgtgctcat
     4921 cgacggcgaa gacggtctga tgaccacgct tgccaaatgc tgcaaacccg cgccgcccga
     4981 cgacattgtc ggcttcgtta cccgcgatcg cggcatttcg gtacaccgca aaacctgccc
     5041 ctctttccga cacctcgccg aacacgcgcc cgaaaaagta ctggacgcaa gttgggcggc
     5101 gttgcaggaa ggacaagtgt tcgccgtcga tatcgaaatc cgcgcccaag accgctccgg
     5161 gcttttgcgc gacgtatccg acgcgctcgc ccgccacaaa ctcaacgtta ccgccgtgca
     5221 aacccagtcc cgcgacttgg aagccagtat gcggttcacg ctcgaagtca aacaggtcaa
     5281 cgacctcccg cgcgtcctcg ccagcctcgg cgacgtcaaa ggcgtattga gcgttacccg
     5341 gctttaaata caaaaatgcc gtctgaaagc cgtataacgc ttcagacggc attttgattg
     5401 acggggtttg ctattttttg ttgcatagtc aattaaaaac aaaatagtac aatactcaac
     5461 tttgaaggtc taaccatggc atactctgcg gacttaagaa acaaagcttt aaactatagt
     5521 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag
     5581 gcgaggcaac accgtactgg tttaaattta atccacgata ttacgaacaa tgcaaaaaca
     5641 tcagccaaac cgcagcaacg tttaacttgt caagaaacac gctttacctg tggattcgcc
     5701 ttaaaaaaca aacaggcagc ctaaaacatc aagttaccgg tctaaatgcc gtcaaatcgg
     5761 ataggcaaaa accggctcaa tatattgggc aacacc
//
LOCUS       DAXIHS010000102         5591 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXIHS010000102 DAXIHS010000000
VERSION     DAXIHS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.102"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(52..1044)
                     /gene="pdxA"
                     /locus_tag="ACFOF2_001827"
     CDS             complement(52..1044)
                     /gene="pdxA"
                     /locus_tag="ACFOF2_001827"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC7108091.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPTAEAVAAGRLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1204..2631
                     /locus_tag="ACFOF2_001828"
     CDS             1204..2631
                     /locus_tag="ACFOF2_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC7108092.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            2956..4851
                     /gene="mnmG"
                     /locus_tag="ACFOF2_001829"
     CDS             2956..4851
                     /gene="mnmG"
                     /locus_tag="ACFOF2_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC7108093.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            4919..5503
                     /gene="rnhB"
                     /locus_tag="ACFOF2_001830"
     CDS             4919..5503
                     /gene="rnhB"
                     /locus_tag="ACFOF2_001830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC7108094.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT         1278 a         1654 c         1508 g         1151 t
ORIGIN      
        1 cgaacagacc ggattcccgc ctgcgcggaa atgacgggtc ttttatcatc tttaaaggct
       61 gccgcgcgcc atctcgacgg cggtctccac ggcagttatc aggctgccgg aatccgccct
      121 gccggttgcc gccaaatcga gcgcggtgcc gtgatcgacg gaggtgcgga taaagggcag
      181 ccccagcgtg atgttcacgc cctgtccgaa gctgtggtat ttcaacacgg gcagcccttg
      241 gtcgtggtac atcgccaata cggcatccgc accttcgagc ataaacggtt ggaacaaggt
      301 atccgccgga tacgggccgg cgaggtttat cccttcgcgg cgcaggtttt ccaatgcggg
      361 gataatggtg tcggtttctt cgtgtccgag gtgtccgctc tcgccggcgt gggggttgag
      421 tccggcgact aggattttgg gatttttgat gccgaatttg tgttttaagt cgtgatgcaa
      481 aatgcgtgcg acgctttcaa tcagcggttg cgtgatggcg gcggcaacgt ctttcagcgg
      541 caggtgggtc gttacgaggg cgacgcgcag gcctttgccg gcaagcatca tcacaacctg
      601 ccccgtgccg cttttttccg ctagatattc ggtgtgcccg ctgaaaaaac ctgtgcttgc
      661 gcgcgcgtcg ttgatgatgc ctttgtgcag cggcgcggta acgatgccgt cgaaaatgcc
      721 gtctgaaatg cctgcgagcg cggtgtccaa aagttgcagc acataggcgg cgttggcggg
      781 attgagccgc cctgccgcaa ccgcttcggc ggtcggaatg tgcagcactt ccagctcgcc
      841 ttctccgggt gcgccgcctg attccggatc gaagtcgcgc aagacgacgc ttttgcccaa
      901 ggcttcggcg cgcgcgcgca ataggttttt gtcgcccaat accgcgcagc ggcagggcag
      961 gcgtgcaaac gccaagtcca aacaaatatc ggggccgatg ccggcaggct cgccggaagt
     1021 aacggcaaaa acaggctgtt tcatcgtgtt tgctccaaac aaaatgcgat tctaacgccg
     1081 cagccgcgcg gcgatgtaaa tttttctgat tttgttgaca atctgctaga atgggcgttt
     1141 acaaaattta aaccctgctt gcataccgcc aatatgtgcg agtttcaact ttaaggaagc
     1201 gatatgaacg agaactttac cgaatggctg cacggctggg tcggcgccat caacgatccg
     1261 atgtggtcat acttggttta tatgcttttg ggtacggggc ttttcttcac cgtaaccacg
     1321 ggctttgtcc aattccgcct gttcgggcgc agcatcaaag aaatgctcgg cggccgcaaa
     1381 cagggggacg accctcacgg catcacgccg tttcaggcat ttgtaaccgg ccttgccagc
     1441 cgcgtgggcg tgggcaatat cgcgggcgtg gccatcgcca tcaaagtcgg cggaccgggc
     1501 gcggtgtttt ggatgtgggt aaccgcctta atcggtatga gttcggcgtt tgtcgaatct
     1561 tcgctggcgc agctctttaa agtccgcgac tacgacaacc accatttccg gggcggccct
     1621 gcctactaca tcactcaagg gctggggcag aaatggctgg gcgtgttgtt cgccctgagc
     1681 ctgattttct gtttcggctt tgtgtttgaa gcggttcaga ccaataccat tgccgatacc
     1741 gtcaaagcgg cgtggggttg ggagcctcat tatgtcggcg tcgccctggt gattttaacc
     1801 gcgccgatta tcttcggcgg catcaggcgc atatctaaag cggcggaaat cgtcgtcccc
     1861 ctgatggcgg ttttgtacct ctttatcgcg cttttcatca ttttgaccaa tattccgatg
     1921 attccggacg tgttcggtca gattttttcg ggcgcgttca aattcgacgc ggcagcaggc
     1981 ggcttactcg gcggtctgat ttcgcaaacg atgatgatgg gcatcaaacg cggcctgtat
     2041 tccaacgagg cgggtatggg ttccgcgccg aacgccgccg ccgccgccga agtgaaacac
     2101 cctgtttcgc aaggtatgat tcaaatgctg ggcgtgtttg tcgataccat catcgtttgt
     2161 tcttgcaccg ccttcatcat cttgatttac caacagcctt acggcgattt gagcggtgcg
     2221 gcgctgacgc aggcggcgat tgtcagccaa gtggggcaat ggggcgcggg cttcctcgcc
     2281 gtcatcctgt ttatgtttgc cttttccacc gttatcggca actatgccta tgccgagtcc
     2341 aacgtccaat tcatcaaaag ccattggctg attaccgccg ttttccgtat gctggttttg
     2401 gcgtgggtct atttcggcgc ggttgccaat gtgcctttgg tctgggatat ggcggatatg
     2461 gcgatgggca ttatggcgtg gatcaacctt gtcgccatcc tgctgctctc gcccttggcg
     2521 tttatgctgc tgcgcgatta caccgccaag ctgaaaatgg gcaaagaccc cgagttcaaa
     2581 ctttccgaac atccgggcct gaaacgccgt atcaaatccg acgtttggta aatcccgccc
     2641 ttaccagagc cgcttccccg cgaagcggct tttccctttc cgcacactgt aaaaacaggg
     2701 cgaacaagcg tacaatccca accctttact tttgaatcca tttcgttttt cagacggcat
     2761 attgaatata gtggtttaac aaaaatcggg acaaggcggc gaagccgcag acagtacaga
     2821 tagtacagaa ccgattcact tggtacttca gcaccttaga gaatcgttct ctttgagcta
     2881 aggcgaggca acgctgtact ggtttttgtt aatccactat aaatgccgtc tgaaacaccg
     2941 tcaggcaata cacacatgac ccacatgatt taccccaaaa cctacgacgt tatcgtcgtc
     3001 ggcggcggac acgccggcac agaagccgcg ctcgccgccg cccgtatggg cgcgcagacg
     3061 cttttgctca cacacaatat tgaaacgctc ggacaaatgt cgtgcaaccc ttccatcggc
     3121 ggcatcggca aagggcattt ggtgcgcgaa ctcgacgcgc tcggcggcgc gatggcgttg
     3181 gcaaccgaca aatccggcat ccagttccgc cgtctgaacg ccagcaaagg cgcggcagta
     3241 cgggccacgc gcgcgcaggc ggatcgtatc ctatataaag ccgccatccg cgaaatgttg
     3301 gaaaaccaag aaaacctcga ccttttccaa caagccgtcg aagacgtaac gctcgatggc
     3361 gaccgcattt caggcgtaat caccgcgatg ggcgtggagt ttaaagcacg cgccgtggtg
     3421 ttgaccgcag gcacgttttt gtccggcaaa atccacatcg gtttggaaaa ctacgagggc
     3481 ggacgcgccg gcgaccccgc cgccaaatcg ctgggcggac gtttgcgcga attgaacctg
     3541 ccgcaaggcc gtctgaaaac cggcacgccg ccgcgcatcg acggacgcac gattgacttc
     3601 tcccaactga ccgaacagcc cggcgacacg cccgttcccg tcatgtccgt acgcggcaac
     3661 gccgaaatgc acccgcgcca agtgtcctgc tggattacgc ataccaacac gcaaacccac
     3721 gacatcatcc gctcaggctt cgaccgcagc ccgatgttta ccggcaaaat cgaaggcgtg
     3781 ggcccgcgtt attgtccgtc tatcgaagac aaaatcaacc gcttcgccga caaagacagc
     3841 caccagattt tcctagaacc cgaaggcctg actacgcacg aatactaccc caacggtatc
     3901 tccaccagcc tgccgttcga catccaaatc gcgctcgtcc gcagtatgaa aggtttggaa
     3961 aacgcccata tcctgcgccc cggctacgcc atcgaatacg actacttcga tccgcgcaac
     4021 ctcaaagcaa gcctcgaaac caaaaccatt gccggattgt ttttcgccgg gcaaatcaac
     4081 ggtacgacag gttatgaaga agccgccgcg caaggactgc tggcaggcgc gaacgccgtg
     4141 caatacgtcc gcgaacaaga cccgctcctg ctgcgccgcg aacaagccta tctcggcgta
     4201 ttggtggacg acctcatcac caaaggcgtg aacgaaccct accgaatgtt caccagccgt
     4261 gccgaatacc gcctgcaact cagggaagac aacgccgata tgcgcctgac cgaagacggc
     4321 tacaaaatcg gcttggtggg tgaagcgcaa tggcgcatgt tcaacgaaaa acgcgaagcc
     4381 atcgaacgcg aaatccaacg tttgaaaaca acgtggtaca cgccgcaaaa actcgccgaa
     4441 gacgaacaaa tccgcgtatt cgggcaaaag ctcagccgcg aagccaacct gcacgacctc
     4501 ctgcgccgcc cgaacctcga ctacgccgcg ctgatgacgc tcgaaggcgc gatgccgtct
     4561 gaacgcctct ccgccgaagt ggtcgaacaa gtcgaaatcc aagtcaaata ccaaggctat
     4621 atcgaccgcc aaaacgaaga aatcgacagc cgccgcgaca tcgaaacctt aaagctgccc
     4681 gaggatatcg attacagcaa agttaaaggt ttgtcggcag aagtgcagca aaagctcaac
     4741 cagcacaaac ccgaaaccgt cgggcaggca agccgcattt ccggcgtaac ccctgcggca
     4801 gtggcattgc tgatggtgca tttgaagcgc gggtttaaag acgcgaaata aacacatcgg
     4861 cgcgatgccg tctgaaacac cgttcagacg gcatcgcgcc atcccgacag gaaacatcat
     4921 gcacatactg accgccggcg tggacgaggc agggcgcggg cctttagtcg gcagcgtgtt
     4981 tgccgccgcc gtcatccttc cggaaatatt cgacctgccc ggactgaccg actccaaaaa
     5041 actcagcgag aaaaaacgcg acgcgcttgc cgaaatgata aaaaatcagg cggttgcgtg
     5101 gcacgttgcc gccgcctcgc ccgaagaaat cgccagcctc aacatcctgc acgccaccat
     5161 gctcgcgatg aaacgcgccg ttgacggttt ggctgtgcgt cccgaaagaa tattcatcga
     5221 cggcaaccgc attcccgaac atttgaacat ccctgccgaa gccgtcgtca aaggcgacag
     5281 caaaatcatc gaaatctccg ccgcatccgt tttggcaaag accgcacgcg atgcagaaat
     5341 gtacgcactg gcgcaacgcc atccccaata cggtttcgac aaacacaaag gttacggcac
     5401 gaagcagcat ctggaagccc tcaaacaata cggcgtgctg cccgaacacc gccgagactt
     5461 cgcccccgtc agaaacctgc tcgcgcagca ggccttgttt taaaccggca caaaaatgcc
     5521 gtctgaactt cagacggcat tttcagcatc gcaacttcaa agagaaagaa cccaaaccgt
     5581 cattcccgcg a
//
LOCUS       DAXIHS010000103         5562 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXIHS010000103 DAXIHS010000000
VERSION     DAXIHS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.103"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(52..165)
                     /gene="rrf"
                     /locus_tag="ACFOF2_001831"
     rRNA            complement(52..165)
                     /gene="rrf"
                     /locus_tag="ACFOF2_001831"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(262..3153)
                     /locus_tag="ACFOF2_001832"
     rRNA            complement(262..3153)
                     /locus_tag="ACFOF2_001832"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3559..3634)
                     /locus_tag="ACFOF2_001833"
     tRNA            complement(3559..3634)
                     /locus_tag="ACFOF2_001833"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3599..3601),aa:Ala,seq:tgc)
     gene            complement(3640..3716)
                     /locus_tag="ACFOF2_001834"
     tRNA            complement(3640..3716)
                     /locus_tag="ACFOF2_001834"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3680..3682),aa:Ile,seq:gat)
     gene            complement(3818..5358)
                     /locus_tag="ACFOF2_001835"
     rRNA            complement(3818..5358)
                     /locus_tag="ACFOF2_001835"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1204 a         1620 c         1187 g         1551 t
ORIGIN      
        1 tttccgggca aagcaaaaac ccccgaataa tcgggggttt tctgaaaggg tgtttggcag
       61 tgacctactt tcgcatggaa gaaccacact atcatcggcg ctgagtcgtt tcacggtcct
      121 gttcgggatg ggaaggcgtg ggaccaactc gctatggccg ccaaacttaa actgttacaa
      181 atcggtaaag ccttaatcaa tatattcggt aatgactgaa tcagtcagta agcttttatc
      241 tcttgaagtt cttcaaatga tagagtcaag cctcacgagc aattagtatg ggttagcttc
      301 acgcgttacc gcgcttccac accccaccta tcaacgtcct ggtctcgaac gactctttag
      361 tgcggttaaa ccgcaaggga agtctcatct tcaggcgagt ttcgcgctta gatgctttca
      421 gcgcttatct cttccgaact tagctacccg gctatgcaac tggcgttaca accggtacac
      481 cagaggttcg tccactccgg tcctctcgta ctaggagcag cccccgtcaa acttccaacg
      541 cccactgcag atagggacca aactgtctca cgacgtttta aacccagctc acgtaccact
      601 ttaaatggcg aacagccata cccttgggac cgactacagc cccaggatgt gatgagccga
      661 catcgaggtg ccaaactccg ccgtcgatat gaactcttgg gcggaatcag cctgttatcc
      721 ccggagtacc ttttatccgt tgagcgatgg cccttccata cagaaccacc ggatcactat
      781 gtcctgcttt cgcacctgct cgacttgtcg gtctcgcagt taagctacct tttgccattg
      841 cactatcagt ccgatttccg accggaccta ggtaaccttc gaactcctcc gttacgcttt
      901 gggaggagac cgccccagtc aaactgccta ccatgcacgg tccccgaccc ggatgacgag
      961 tctgggttag aacctcaaag acaccagggt ggtatttcaa ggacggctcc acagagactg
     1021 gcgtctctgc ttcaaagcct cccacctatc ctacacaagt gacttcaaag tccaatgcaa
     1081 agctacagta aaggttcacg gggtctttcc gtctagcagc gggtagattg catcttcaca
     1141 accacttcaa cttcgctgag tctcaggagg agacagtgtg gccatcgtta cgccattcgt
     1201 gcgggtcgga acttacccga caaggaattt cgctacctta ggaccgttat agttacggcc
     1261 gccgtttact ggggcttcga tccgatgctc tcacatcttc aattaacctt ccagcaccgg
     1321 gcaggcgtca caccctatac gtccactttc gtgttagcag agtgctgtgt ttttaataaa
     1381 cagtcgcagc cacctattct ctgcgaccct ccagggctta cggagcaagt ccttaacctt
     1441 agagggcata ccttctcccg aagttacggt atcaatttgc cgagttcctt ctcctgagtt
     1501 ctctcaagcg ccttagaatt ctcatcctgc ccacctgtgt cggtttgcgg tacggttcaa
     1561 ttcaaactga agcttagtgg cttttcctgg aagcgtggta tcggttactt catgtccgta
     1621 gacactcgtc atcacttctc ggtgttaaga agacccggat ttgcctaagt cttccaccta
     1681 ccggcttaaa caagctattc caacagcttg ccaacctaac cttctccgtc cccacatcgc
     1741 atttgaatca agtacaggaa tattaacctg tttcccatcg actacgcatt tctgcctcgc
     1801 cttaggggcc gactcaccct acgccgatga acgttgcgta ggaaaccttg ggctttcggc
     1861 gagcgggctt ttcacccgct ttatcgctac tcatgtcaac attcgcactt ctgatacctc
     1921 cagcacactt tacaatgcac cttcatcggc ctacagaacg ctcccctacc atgccagtaa
     1981 actggcatcc gcagcttcgg ttatagattt gagccccgtt acatcttccg cgcaggacga
     2041 ctcgaccagt gagctattac gctttcttta aatgatggct gcttctaagc caacatcctg
     2101 gctgtctggg ccttcccact tcgtttacca cttaatctat cattggggac cttagctggc
     2161 ggtctgggtt gtttccctct tgacaacgga cgttagcacc cgctgtctgt ctcccgagga
     2221 accacttgat ggtattctga gtttgccatg ggttggtaag ttgcaataac cccctagcca
     2281 taacagtgct ttacccccat cagtgtcttg ctcgaggcac tacctaaata gttttcgggg
     2341 agaaccagct atctccgagt ttgtttagcc tttcacccct atccacagct catccccgca
     2401 ttttgcaaca tgcgtgggtt cggtcctcca gtacctgtta cggcaccttc aacctggcca
     2461 tggatagatc actcggtttc gggtctacac ccagcaactc atcgccctat taagactcgg
     2521 tttccctacg cctcccctat tcggttaagc tcgctactga atgtaagtcg ttgacccatt
     2581 atacaaaagg tacgcagtca cacctctagg gcgctcccac tgtttgtatg catcaggttt
     2641 caggttctgt ttcactcccc tcccggggtt cttttcgcct ttccctcacg gtactggttc
     2701 actatcggtc gatgatgagt atttagcctt ggaggatggt ccccccatat tcagacagga
     2761 tttcacgtgt cccgccctac ttttcgtacg cttagtaccg ctgttgagat ttcgaatacg
     2821 ggactgtcac ccactatggt caagcttccc agcttgttct tctatctcga cagttattac
     2881 gtacaggctc ctccgcgttc gctcgccact acttgcggaa tctcggttga tttcttttcc
     2941 tccgggtact tagatggttc agttctccgg gttcgcttct ctaagtctat gtattcaact
     3001 taggatactg cacagaatgc agtgggtttc cccattcgga catcgcggga tcattgcttt
     3061 attgccagct cccccgcgct tttcgcaggc ttacacgtcc ttcgtcgcct atcatcgcca
     3121 aggcatccac ctgatgcact tattcacttg actctatcat ttcaagaact tctttgactt
     3181 tgcctaacat tccgttgact agaacatcag acttgaattt cctactttga taaagcttac
     3241 tgctttgttg tgtcttaatc ctgccttttg tgtttcagga ttaagtcgat acaatcatca
     3301 cccaaatact gtgtttgttt tcttttctct cgcgagagat ttttatcctt tgcaaagaat
     3361 aaaaaatcaa aacaaacgct ttgtctttgt ttgttgattt cggctttcca atttgttaaa
     3421 gatcgatgcg ttcgatattg ctatctactg tgcaaatcaa aacgagctga ttattatatc
     3481 agcattttgt tcttggtcaa gtgtgacgtc gccctgaatg gattctgttc cattcttccg
     3541 ttttgatttg tacagtattg gtggaggcaa acgggatcga accgatgacc ccctgcttgc
     3601 aaagcaggtg ctctaccaac tgagctatgc ccccgttctt ggtgggtctg ggaggacttg
     3661 aacctccgac cccacgctta tcaagcgtgt gctctaacca gctgagctac aaacccggat
     3721 tctcttctta agcgaatctt gccttcactc aagcttcttc cgcatctttt tcagtttacc
     3781 gataagtgtg aatgcctaaa gcctcttctt tctctagaaa ggaggtgatc cagccgcagg
     3841 ttcccctacg gctaccttgt tacgacttca ccccagtcat gaagcatacc gtggtaagcg
     3901 gaccccttgc ggttacccta cctacttctg gtatccccca ctcccatggt gtgacgggcg
     3961 gtgtgtacaa gacccgggaa cgtattcacc gcagtatgct gacctgcgat tactagcgat
     4021 tccgacttca tgcactcgag ttgcagagtg caatccggac tacgatcggt tttgtgagat
     4081 tggctccgcc tcgcggcttg gctaccctct gtaccgacca ttgtatgacg tgtgaagccc
     4141 tggtcataag ggccatgagg acttgacgtc atccccacct tcctccggct tgtcaccggc
     4201 agtctcatta gagtgcccaa ctgaatgatg gcaactaatg acaagggttg cgctcgttgc
     4261 gggacttaac ccaacatctc acgacacgag ctgacgacag ccatgcagca cctgtgttac
     4321 ggctcccgaa ggcactcctc cgtctctgga ggattccgta catgtcaaga ccaggtaagg
     4381 ttcttcgcgt tgcatcgaat taatccacat catccaccgc ttgtgcgggt ccccgtcaat
     4441 tcctttgagt tttaatcttg cgaccgtact ccccaggcgg tcaatttcac gcgttagcta
     4501 cgctactaag caatcaagtt gcccaacagc taattgacat cgtttagggc gtggactacc
     4561 agggtatcta atcctgtttg ctacccacgc tttcgggcat gaacgtcagt gttgtcccag
     4621 gaggctgcct tcgccatcgg tattcctcca catctctacg catttcactg ctacacgtgg
     4681 aattctacct ccctctgaca cactcgagtc acccagttca gaacgcagtt cccgggttga
     4741 gcccggggat ttcacatcct gcttaagtaa ccgtctgcgc ccgctttacg cccagtaatt
     4801 ccgattaacg ctcgcaccct acgtattacc gcggctgctg gcacgtagtt agccggtgct
     4861 tattcttcag gtaccgtcat cagccgctgg tattagcaac agccttttct tccctgacaa
     4921 aagtccttta caacccgaag gccttcttca gacacgcggc atggctggat caggcttgcg
     4981 cccattgtcc aaaattcccc actgctgcct cccgtaggag tctgggccgt gtctcagtcc
     5041 cagtgtggcg gatcatcctc tcagacccgc tactgatcgt cgccttggta ggcctttacc
     5101 ccaccaacta gctaatcaga tatcggccgc tcgaatagcg caaggcccaa aggtcccctg
     5161 ctttctctct caagacgtat gcggtattag ctgatctttc gatcagttat cccccactac
     5221 tcggtacgtt ccgatatgtt actcacccgt tcgccactcg ccacccgaga agcaagcttc
     5281 tctgtgctgc cgtccgactt gcatgtgtaa agcatgccgc cagcgttcaa tctgagccag
     5341 gatcaaactc ttatgttcaa tctctaactt tttaacttct ggtctgcttc aaagaaacca
     5401 acaggacaat gttcaaaaca ttatcttgtc tgtctttcaa acagtgtgag gctcaaggca
     5461 ctcacactta tcggtaatct gttttgttaa agagcgttgc gaattataaa gtatctcttc
     5521 cgcctgtcaa gatatctctc gatatcccca acattctgtg ct
//
LOCUS       DAXIHS010000104         5310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXIHS010000104 DAXIHS010000000
VERSION     DAXIHS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.104"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            169..2415
                     /locus_tag="ACFOF2_001836"
     CDS             169..2415
                     /locus_tag="ACFOF2_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC7108095.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAPTLSDSKS
                     SNPADKPAPAPAEPSVEITPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLS
                     FKEGDVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGK
                     DEIKWTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGK
                     AVGIDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRD
                     RNGPYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEYLFFHTDADQRLEGGFFG
                     DNGEELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEA
                     ADKKARPFEVSPMPDFGHPDKLLVEGREIPLVSQEKTIKLADGREMTVRACCDFLTYV
                     KLGRIKTDRPAVKPKVQDEEGDEEDTGVDSVEEGEIGIGDEDEGTEDEAVEDGEESEE
                     EISEDDNGEDEEEIAEDEGEEEPEEESPEEGNGSSGSIPPAPEASKGRDIDLFLKGIR
                     TAETDIPHSGTAHYTGTWEARIGTPIQWDNQADKTAKAKFTVDFGGKSISGTLTEENG
                     VEPAFYIEKGVIDGNGFHATAHTRDTGINLSGTGSTNPQRFKASNLRVEGGFYGPQAE
                     ELGGIIFNNDGKSLGITEGTENKVEADVEAEAEAEVEAGVEQLKPEVKPQFGVVFGAK
                     KDMQEVEK"
     gene            2412..5243
                     /gene="lbpA"
                     /locus_tag="ACFOF2_001837"
                     /gene_synonym="tbpA"
     CDS             2412..5243
                     /gene="lbpA"
                     /locus_tag="ACFOF2_001837"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC7108096.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIQREKTEPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEKNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1506 a         1226 c         1434 g         1144 t
ORIGIN      
        1 gggaatgacg ggatgcaggt tcgtgggaat gacgggatgt atctgtactg tctgcggctc
       61 gtcgccttgt ccggattttt gttaattcac tataaaaacg gcttgatatt atctgtacat
      121 attaatataa tgataattat tattaatcaa ataggaggaa aagtagggat gtgtaaaccg
      181 aattatggcg gcattgtctt gttgccctta cttttggcat cttgtatcgg cggcaatttc
      241 ggcgtacagc ctgttgtcga atcaacgccg accgcgccaa ctctgtcaga ttccaaatct
      301 tccaatcctg cggataagcc tgctccagct cctgccgagc cttcggtaga aatcacgccg
      361 gtcaaccggc ccgccgtcgg tgcggcaatg cggctgccaa ggcggaatac tgcttttcat
      421 cgtgaagatg gcacggtaat tcccgatagc aaacaagcag aagaacagct gtcgtttaaa
      481 gaaggtgatg ttctgttttt atacggttca aaagaaaata aacttcaaca acttaaaagc
      541 gaaattcata aacgtgatcc cgatgtagaa attaggacat cagaaaatga aaataaaaaa
      601 tatgattata aatttgtaga tgcaggttat gtatatgtaa agggaaaaga tgaaattaag
      661 tggacttcag atcacaagca gttttccaac cgcttaggtt atgacggttt tgtatattat
      721 tccggagaac gtccttccca atctttaccg agtgcgggaa cggtggaata ttctggtaac
      781 tggcaatata tgaccgatgt caaacgtcat cgagcaggta aggcggttgg cattgacaat
      841 ttgggttata tcacatttta tggtaacgat gttggtgcaa cttcttatgc ggctaaggat
      901 gtcgacgaaa gggaaaagca tcctgccaaa tatacggttg atttcgacaa aaaaatccta
      961 aagggcgagc tgattaaaaa tcagtatgtg cgagatagaa atggtcccta taatccgctg
     1021 accatttacg acattactgc aacattggac ggcaaccgct ttaccggcag tgccaaagtt
     1081 aacaccaagt tgaagacgag ccacgctgat aaagaatatt tgtttttcca taccgatgcc
     1141 gatcagcggc ttgagggcgg ttttttcggt gataacggag aagagcttgc cggacggttt
     1201 atcagcaacg acaaaagcgt attcggcgta ttcgcaggca aacaaaaaac agagacagca
     1261 aacgcatcag atacaaatcc tgccctgccg tctggaaaac acaccaaaat cttggattct
     1321 ctaaaaattt ccgttgacga ggcggctgat aaaaaagccc gtccgtttga ggtttcccct
     1381 atgcccgatt ttggtcatcc cgacaagctt cttgtcgaag ggcgtgaaat tcctttggtt
     1441 agccaagaga aaaccatcaa gcttgccgac ggcagggaaa tgaccgtccg tgcttgttgc
     1501 gactttttga cctatgtgaa actcggacgg ataaaaaccg atcgtcccgc cgtcaaaccg
     1561 aaggtgcagg atgaagaggg ggacgaagag gatacaggcg ttgatagcgt cgaagaaggc
     1621 gaaatcggaa tcggcgatga agacgaaggc acggaagacg aagccgtaga agacggcgaa
     1681 gaaagcgaag aagaaatttc cgaagatgat aacggtgaag acgaagaaga aatcgccgaa
     1741 gacgaaggcg aagaagaacc cgaagaagaa tcgccggaag aaggaaacgg cagttcaggc
     1801 agcatcccgc ctgccccgga agcctctaaa ggcagggaca tcgacctttt cctgaaaggt
     1861 atccgcacgg cggaaacgga tattccgcat agcggaacgg cgcattatac cggcacttgg
     1921 gaagcgcgta tcggcacacc cattcaatgg gacaatcagg cggataaaac cgcaaaagca
     1981 aaatttaccg ttgatttcgg cgggaaatcg atttctggaa cgctgacgga ggaaaacggt
     2041 gtagaacctg ctttctatat tgaaaaaggt gtgattgatg gcaacggttt ccacgcgaca
     2101 gcacacactc gggataccgg catcaatctt tcgggaactg gttcgactaa tcctcaacgt
     2161 ttcaaagcca gtaatcttcg tgtagaagga ggattttacg gcccgcaggc ggaggaattg
     2221 ggcggtatta ttttcaataa tgatgggaaa tctcttggta taactgaagg tactgaaaat
     2281 aaagttgaag ctgatgttga agctgaagct gaagctgaag ttgaagctgg tgttgaacag
     2341 ttaaaacctg aagttaaacc ccaattcggc gtggtattcg gtgcgaagaa agatatgcag
     2401 gaggtggaaa aatgaataag aaacacggtt ttcagcttac tctgactgcc ttggctgttg
     2461 cagccgcttt cccatcatat gcggcaaacc ccgaaacggc gacacctgat gcggcgcaga
     2521 cccaatccct gaaagaggtt accgtccgtg ccgccaaagt gggacggcga tcgaaagagg
     2581 cgaccggttt gggcaaaatc gtcaaaacgt cggaaacttt gaacaaagaa caggtactcg
     2641 gtatccgcga cctgacgcgc tacgatccgg gcgtggcggt tgtcgaacag ggcaacggcg
     2701 cgagcggcgg ctactcgata cgcggcgtgg ataaaaaccg tgtggcggtt tcggttgacg
     2761 gcgttgccca aatacaggcg tttaccgtgc agggatcgtt gagcggatac ggcggacgcg
     2821 gcggcagcgg tgcaatcaac gaaatcgaat atgaaaacat cagcacggtg gaaatcgaca
     2881 aaggtgccgg ttcgtccgat cacggcagcg gcgcactcgg cggcgcggtc gccttccgca
     2941 ccaaagaggc ggcagacctg atttcagacg gcaaaagttg ggggatacag gcaaaaaccg
     3001 cctacggcag taaaaaccgc caatttatga agtcgctcgg cgcggggttc agcaaagacg
     3061 gttgggaagg gctgttaatc cgaaccgaac gtcaggggcg ggaaacgcgc ccgcacggcg
     3121 atattgcgga cggggtggca tacggcataa accgtttgga cgcgttccgc cagacatacg
     3181 atattcaacg cgagaaaaca gagccatttt tctcagtaga gggcgagcgt gaatccaagc
     3241 ccgtggcaaa attggcgggc tacgggaaat atttgaacaa ccagctcaac cgctgggtaa
     3301 aagaacgtat tgaaaaaaat cagcctttaa gtgctgaaga agaggcgcag gtgcgggagg
     3361 cgcaggcgcg ccacgaaaac ctgtccgccc aagcttacac gggcggcggc aggatattgc
     3421 ccgatccgat ggattaccgc agcggctctt ggcttgccaa gctgggctac cgcttcggcg
     3481 gcaggcatta tgtcggcggc gtgtttgagg ataccaaaca gcgttacgac atccgcgata
     3541 tgacggaaaa acagtattac ggtacggacg aggcggacaa gtttagagac aagagcgggg
     3601 tgtacgacgg cgacgatttc cgcgacggct tgtattttgt gccgaatata gaagagtgga
     3661 agggcgataa aaagttggtc aggggcatag gtttgaaata ttcccgcacc aaatttattg
     3721 acgaacatca ccgccgccgc cgtatgggtt tgctgtaccg ttatgaaaac gaagcgtatt
     3781 ctgacaattg ggcggacaag gcggtgttgt cgtttgacaa acagggcgtg gcaaccgaca
     3841 acaacacgct gaagctgaat tgtgccgtgt atcctgctgt ggacaaatcc tgccgcgcgt
     3901 cggcggacaa accgtattcc tacgacagca gcgaccgttt ccactaccgc gaacagcaca
     3961 atgttttgaa tgcctcgttt gagaaatcgc tgaaaaacaa atggacgaaa caccatctga
     4021 ctttgggctt cggttacgat gcttccaaag cgatctcccg ccccgaacag ctttcccaca
     4081 atgcggcaag gatttcggaa tccacgggat tcgatgataa aaatcaagat aagtatcttt
     4141 tgggtaagcc cgaagtcgtc gaagggtcgg tctgcggcta catcgaaact ttgcgttccc
     4201 gcaaatgcgt gccaagaaaa atcaacggca gcaatatcca catttctttg aacgaccgtt
     4261 tttcaatcgg caaatatttt gatttcagct tgggcggcag gtacgaccgg aaaaacttca
     4321 ccacgtcgga agaactcgtc cgcagcgggc ggtatgttga ccgttcgtgg aacagcggca
     4381 tcgtgttcaa accgaaccgg catttttccg tgtcttaccg cgcctccagc ggcttcagaa
     4441 cgccctcatt ccaagaactt ttcgggatag acatttatca cgattatccg aaaggctggc
     4501 agcgtcccgc cctgaaatcg gaaaaggcag ccaaccgcga aatcggtttg cagtggaagg
     4561 gcgatttcgg ctttttggaa atcagcagct tccgcaaccg ctataccgat atgattgccg
     4621 ttgccgatca caaaaccaaa ttgccgaatc aggcaggaca attgacagag attgatatac
     4681 gcgattatta caatgcccaa aatatgtcgc tccaaggcgt taatatattg ggaaaaatcg
     4741 actggaacgg tgtgtacggc aaactgcccg aaggtttgta taccacattg gcgtacaacc
     4801 gtatcaaacc gaaatcggta tccaaccggc cgggactgtc cctccgcagc tatgctttgg
     4861 atgcggtaca gccgtcgcgt tatgttttgg ggttcggata cgaccagccc gaggggaaat
     4921 ggggcgcaaa cattatgctg acctattcca aagggaaaaa ccctgacgag cttgcttatc
     4981 tggcaggcga tcaaaaacga tattcgacaa aaagagcgtc gtcttcttgg tcgacggcag
     5041 acgtttccgc ctatctgaat ctgaaaaaac ggctgacctt gagggcggct atctacaata
     5101 tcggcaacta ccgctacgtt acttgggaat ccttgcgcca gaccgcggaa agcacggcaa
     5161 accggcacgg cggcgacagc aactatggaa ggtatgccgc accgggcagg aacttcagcc
     5221 tcgcgctcga aatgaagttt taaaggaaat accgtctgaa agcttgatct gcaccccaaa
     5281 agtcggacta aaccgccaac tgattaaggt
//
LOCUS       DAXIHS010000105         5246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXIHS010000105 DAXIHS010000000
VERSION     DAXIHS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.105"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            222..2366
                     /locus_tag="ACFOF2_001838"
     CDS             222..2366
                     /locus_tag="ACFOF2_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC7108097.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPKAQKDQGGYGFAMRLKRRNWYPQAKEDEVKLNESDWETTGLPTEPKKLP
                     LKQESVISKVQANNGDNNIYTSPYLTPSNHPNGNTGNGINQPKNEVKDYKDFKYVYSG
                     WFYKHARNEIIRENSSIKGAKNGDDGYIFYHGKEPSRQLPASGTVTYKGVWHFATDVK
                     KSQNFRDIIQPSKKQGDRYSGFSGDDDEQYSNKNESMLKDGQEGYGFTSNLEVDFNNK
                     KLTGKLIRNDKVTNATTGGKHATQYYSLEAQVTGNRFNGKATATDKPGTGETKQHPFV
                     SDSSSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKTGNGAAASDGAGAAASGG
                     AAAMPSENGKLTTVLDAVELTHGGKAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNT
                     NQGTNGGTAFTRKFDYTPKSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYEVEVCCS
                     NLNYLKYGLLTRKTAGNTGEGGNGSPTAAAQTAQGAQSMFLQGERTDEKEIPSEQNVV
                     YRGSWYGHIANGTSWSGNASNATSGNKADFTVNFGEKKINGTLTAENRQAATFTIEGT
                     IQGNGFSGTAKTADSGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEELGGWFAYPGD
                     KQTENATVASGNGNSASSATVVFGAKRQQLVQ"
     gene            2453..5197
                     /gene="tbpA"
                     /locus_tag="ACFOF2_001839"
     CDS             2453..5197
                     /gene="tbpA"
                     /locus_tag="ACFOF2_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC7108098.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDDNQYAHFVVEEECESRNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYHQSANRAYSSKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTGQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIENGKETAKGDPAYLNAQSARITGINVLGK
                     IDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1499 a         1310 c         1368 g         1069 t
ORIGIN      
        1 aggttcgtgg gaatgacgtg gtgcaggttc gtgggaatga cgggatatac gtgggaatga
       61 cgggatatag gtttccgtgc ggacgcgttc ggattcccgc ctgcgctgga atgacgggat
      121 tttggtgtat tccctaaaaa aaaaataaaa aagcatttgc aaatttgtta aaaataaata
      181 aaataataat ctttatcatt ctttaattga attgggttta tatgaacaat ccattggtaa
      241 atcaggctgc tatggtgctg ccggtgtttt tgttgagtgc ttgtctgggc ggaggcggcg
      301 gcagtttcga tcttgattct gtcgataccg aagccccgcg tcccgcgcca aaatatcaag
      361 atgtttcttc cgaaaagccg aaagcccaaa aagaccaagg cggatacggt tttgcgatga
      421 ggttgaaacg gaggaattgg tatccgcagg caaaagaaga tgaggttaaa ctgaacgaga
      481 gtgattggga gacgacagga ttgccgacag aacccaagaa actgccatta aaacaagaat
      541 ccgtcatttc aaaagtacaa gcaaacaatg gcgacaacaa tatttacact tccccctatc
      601 tcacaccatc aaaccatcca aacggcaaca ctggcaacgg tataaaccaa ccaaaaaatg
      661 aagtaaaaga ttataaagat ttcaaatatg tttattccgg ctggttttat aaacatgcta
      721 gaaacgaaat cataagagaa aacagctcaa ttaagggtgc aaagaacggc gacgacggct
      781 atatctttta tcacggcaaa gaaccttccc gacaacttcc cgcttctgga acagttacct
      841 ataaaggtgt gtggcatttt gcgaccgatg tcaaaaaatc ccaaaatttt cgcgatatta
      901 tccagccttc gaaaaaacaa ggcgacaggt atagcggatt ttcgggcgat gatgatgaac
      961 aatattctaa taaaaacgaa tccatgctga aagatggtca agagggttat ggttttacct
     1021 caaatttaga agtggatttc aataataaaa aattgacggg taaattgata cgcaatgata
     1081 aagttacaaa cgctactact ggcggcaaac atgccaccca atattacagc cttgaggctc
     1141 aagtaacagg caaccgcttc aacggcaagg caacggcaac cgacaaaccc ggcactggag
     1201 aaaccaaaca acatcccttt gtttccgact cgtcttcttt gagcggcggc tttttcggcc
     1261 cgaagggtga ggaattgggt ttccgctttt tgagcgacga taaaaaagtt gcggttgtcg
     1321 gcagcgcgaa aaccaaagac aaaacgggaa atggcgcggc ggcttcagac ggcgcaggtg
     1381 cggcagcatc gggcggtgcg gcagctatgc cgtctgaaaa cggtaagctg accacggttt
     1441 tggatgcggt cgagctgacg cacggcggca aagcaatcaa aaatctcgac aacttcagca
     1501 atgccgccca actggttgtc gacggcatta tgattccgct cctgcccgag gcttccgaaa
     1561 gcgggaatac caatcaaggt acaaacggcg gaacagcctt tacccgcaaa tttgattaca
     1621 cgccgaaaag cgatgaaaaa gacacccaag caggtacggc ggcgaatggc gatcaagccg
     1681 cttcaaatac ggcaggtgat accaatggca aaacaaaaac ctatgaagtc gaagtctgct
     1741 gttccaacct caattatctg aaatacgggt tgctgacgcg caagactgcc ggcaatacgg
     1801 gggaaggcgg caacggcagc ccaaccgccg ccgcccaaac ggcacagggt gcacaaagta
     1861 tgttcctcca aggcgagcgc accgatgaaa aagagattcc aagcgagcaa aacgtcgttt
     1921 atcgggggtc ttggtacggg catattgcca acggcacaag ctggagcggc aatgcttcca
     1981 atgcaacgag tggcaacaag gcggacttta ccgtgaattt tggcgagaaa aaaattaacg
     2041 gcacgttaac cgctgaaaac aggcaggcgg caacctttac cattgaggga acgattcagg
     2101 gcaacggttt ttccggtacg gcaaaaactg ctgactcagg ctttgatctc gatcaaagca
     2161 ataccaccgg cacgcctaag gcatatatca cagatgccaa ggtgcagggc ggtttttacg
     2221 ggcctaaagc cgaagagttg ggtggatggt ttgcctatcc gggcgataaa caaacggaaa
     2281 atgcaacagt tgcatccggc aatggaaatt cagcaagcag tgcaactgtc gtattcggtg
     2341 cgaaacgcca acagcttgtg caataagcac ggctgccgaa caatcgagaa taaggcttca
     2401 gacggcatcg ctccttccga tgccgtctga aagcgaagag tagggaaaca ctatgcaaca
     2461 gcaacatttg ttccgattaa atattttatg cctgtcttta atgactgcgc tgcccgttta
     2521 tgcagaaaat gtgcaagccg gacaagcaca ggaaaaacag ttggatacca tacaggtaaa
     2581 agccaaaaaa cagaaaaccc gccgcgataa cgaagtaacc ggtttgggca aattggtcaa
     2641 aaccgccgac accctcagca aggaacaggt actcgatatc cgcgacctga ctcgctacga
     2701 ccccggcatc gccgtcgtcg aacaggggcg cggcgcaagc tcgggctatt cgatacgcgg
     2761 tatggacaaa aaccgcgttt ccttgacggt agacggcttg gcgcaaatac agtcctacac
     2821 cgctcaggcg gcattgggcg ggacgaggac ggcgggcagc agcggcgcaa tcaatgaaat
     2881 cgagtatgaa aacgtcaagg ctgtcgaaat cagcaaaggc tcaaactcgg tcgaacaagg
     2941 cagcggcgca ttggcgggtt cggtcgcatt tcaaaccaaa accgccgacg atgttatcgg
     3001 ggaaggcagg cagtggggga ttcagagtaa aaccgcctat tccggcaaaa accgggggct
     3061 tacccaatcc atcgcgctgg cggggcgcat cggtggtgcg gaggctttgc tgatccacac
     3121 gggtcggcgc gcgggggaaa tccgcgccca cgaagccgcc ggacgcggcg ttcagagctt
     3181 caacaggctg gtgccggttg aagacgacaa ccaatacgct catttcgtgg tcgaagaaga
     3241 atgcgaaagc agaaattacg aaacgtgtaa agcgaatccg aaaaaagatg ttgtcggcaa
     3301 agacgaacgt caaacggttt ccacccgaga ctacacgggt cccaaccgct tcctcgccga
     3361 tccgctttca tacgaaagcc ggtcgtggct gttccgcccg ggttttcgtt ttgaaaacaa
     3421 acggcactac atcggcggca tactcgaacg cacgcaacaa actttcgaca cgcgcgatat
     3481 gacggttccg gcattcctga ccaaggcggt ttttgatgaa aataaaaaac aggcgggttc
     3541 tttgtccggc aacggcaaat acgcgggcga ccacaaatac ggcggactgt ttaccaacgg
     3601 cgaaaatggt gcgctggtgg gcgcggaata cggtacgggc gtgttttacg acgagacgca
     3661 caccaaaagc cgctacggtt tggaatatgt ctataccaat gccgataaag acacttgggc
     3721 ggattatgcc cgcctctctt atgaccggca gggtatcggt ttggacaatc attttcagca
     3781 gacgcactgt tctgccgacg gttcggacaa atattgccgt ccgagtgccg acaagccgtt
     3841 ttcctattac aaatccgacc gcgtgattta cggggaaagc cacaggctct tgcaggcggc
     3901 atttaaaaaa tccttcgata ccgccaaaat ccgccacaac ctgagcgtga atctcgggtt
     3961 tgaccgcttt ggctctaatc tccgccatca ggattattat catcaaagtg ccaaccgcgc
     4021 ctattcgtcg aaaacgcccc ctaaaaacgg caatcaaaac agccccaacg gcgacaagag
     4081 caaaccctat tgggtcagca taggcggggg aaatgtggtt acggggcaaa tctgccgctt
     4141 gggcaacaat acttatacgg actgcacgcc gcgcagcatc aacggcaaaa gctattacgc
     4201 ggcagttcgg gacaatgtcc gtttgggcag gtgggcggat gtcggcgcgg gcttgcgcta
     4261 cgactaccgc agcacgcatt cggacgacgg cagcgtttcc accggcacgc accgcaccct
     4321 gtcctggaac gcaggcatcg tcctcaaacc tgccgactgg ctggatttga cttaccgcac
     4381 ttcaaccggc ttccgcctgc cctcgtttgc ggaaatgtac ggctggcggg cgggtgttca
     4441 aagcaaggcg gtcaaaatcg atccggaaaa atcgttcaac aaagaagccg gcatcgtgtt
     4501 taaaggcgat ttcggcaact tggaggcaag ttggttcaac aatgcctacc gcgatttgat
     4561 tgtccggggt tatgaagcgc aaattgaaaa cggcaaagaa acagccaaag gcgacccggc
     4621 ttacctcaat gcccaaagcg cgcggattac cggcatcaat gttttgggca aaatcgattg
     4681 gaacggcgta tgggataaat tgcccgaagg ttggtattct acatttgcct ataatcgtgt
     4741 ccatgtccgc gacatcaaaa aacgcgcaga ccgcaccgat attcaatcac atctgtttga
     4801 tgccatccaa ccctcgcgct atgtcgtcgg cttgggctat gaccaaccgg aaggcaaatg
     4861 gggtgtgaac ggtatgctga cttattccaa agccaaggaa atcacagagt tgttgggcag
     4921 ccgtgctttg ctcaacggca acagccgcaa tacaaaagcc accgcgcgcc gtacccgccc
     4981 ttggtatatt gtggatgtgt ccggttatta cacgattaaa aaacacttca ccctccgtgc
     5041 gggcgtgtac aacctcctca actaccgcta tgttacttgg gaaaatgtgc ggcaaactgc
     5101 cggcggcgca gtcaaccaac acaaaaatgt cggcgtttac aaccgatatg ccgcccccgg
     5161 ccgaaactac acatttagct tggaaatgaa gttttaaacg tccaaacgcc gcaaatgccg
     5221 tctgaaaggc ttcagacggc attttt
//
LOCUS       DAXIHS010000106         5178 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXIHS010000106 DAXIHS010000000
VERSION     DAXIHS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.106"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(358..1170)
                     /locus_tag="ACFOF2_001840"
     CDS             complement(358..1170)
                     /locus_tag="ACFOF2_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC7108099.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            1255..2127
                     /locus_tag="ACFOF2_001841"
     CDS             1255..2127
                     /locus_tag="ACFOF2_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC7108100.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWRAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(2184..3338)
                     /locus_tag="ACFOF2_001842"
     CDS             complement(2184..3338)
                     /locus_tag="ACFOF2_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC7108101.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3383..3751)
                     /locus_tag="ACFOF2_001843"
     CDS             complement(3383..3751)
                     /locus_tag="ACFOF2_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC7108102.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            3925..4368
                     /locus_tag="ACFOF2_001844"
     CDS             3925..4368
                     /locus_tag="ACFOF2_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC7108103.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            complement(4495..5115)
                     /locus_tag="ACFOF2_001845"
     CDS             complement(4495..5115)
                     /locus_tag="ACFOF2_001845"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC7108104.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
BASE COUNT         1174 a         1594 c         1396 g         1014 t
ORIGIN      
        1 agacccctga cgcggcggga atctatcgga aatgactgaa accccgagat tctagattcc
       61 cactttcgtg ggaatgacgt ggtgcaggtt cccgtatgga tggattcgtc attcccgcac
      121 aggcgggaat ccagacctcc gacgcggcgg gaatctatcg gaaatgactg aaacccccga
      181 gattctagat tcccacttcc gtgggaatga cggttcagtt gcgtaggact ggggcgtcga
      241 aaggggcgga ttcgatggat tcgatgaaaa cggtagaaat gtgggattga tggaaatggc
      301 aggctgaagc ccatcctaca gcccgctgat tccccggcac cgatgccgtc tgaaccttca
      361 gacggcatcc aaaacccggt atttcaaggc gggcaggcgg ttgcggacgc tgttcaggcg
      421 gttggcatcg atgtctgccg taacaacgcc ttcgccttcg ggcaatacgt ccaacacgtc
      481 gccccacgga tcgacaatca tgctgtgtcc gaacgtgcgc cgtccgtttt cgtgcaaacc
      541 gccctgtgcc gccgccacga cgtaacattg gttttcgacg gcacgcgcgc gcagcagcag
      601 ctcccaatgc gccttgcccg tcgtgtgcgt aaacgcagcg ggcagcatca atacgtcaaa
      661 cggcagctgg cggcggaaaa attcgggaaa gcggacatcg taacaaatac ctgccgccac
      721 cggcacgcct tctgccgaca agtgcggcac ttccccgccc gcgcggatgg tatcggcttc
      781 ggcatagcgt tcgcccaaac cggaaaagcc gaagaggtgc attttgtggt acagccccgt
      841 ccgtttgccg tcgcgcccgt acaccaataa agtattcatg accttgcccg cctcggggct
      901 ttgcagcggc acggttccgc caaacagcac cacgccgcat tctttcgccg tttcgctcaa
      961 tgccgtctga aagcgtccgc cgcccaaagg ctcggcaagc gcgagtttgt cggtatcgtt
     1021 tgcgcccatc agcacccaat attcgggcag cagcacccaa tccgcaccct gctccgccgc
     1081 ccgtgcgacc aggcgtttca tggcggcgac gttggtttcc ggcgacacgc ccgacaccat
     1141 ctgcacggcg gcaactctga ttttgtccat ttcttctcct ttttcctgcc gaccttgcgg
     1201 catattccgc cgataggcta ccatgacggt actttgcgaa aaccgtttac acaaatgccc
     1261 attttgaacc atattgccct catcggtgta gggctgatcg gcggttcgtt cgttctcgac
     1321 ctcaaaaggc agggactcgt ccgcaccgtt accggtatcg acaccgaccg cgacaacctc
     1381 gaacgtgcat tggaacgcgg cgtgattgac cgggcttccg ttgccatcga cgcggacagc
     1441 atcggcggtg cggacttggt actgattgcc acgcccgtcg ccaccgttcc cgccattttg
     1501 accgcgctgc gccccgtttt gccggaacac acttggattt ccgatgtcgg cagcaccaaa
     1561 tcttcggtca tcgaagcctt ccgccgctgt ctgcccgacc gcctgcaccg ctgcatcgcc
     1621 gcccacccga ttgccggttc ggacagaagc ggtgcgcaag ccgcgcagtt cgggctgttc
     1681 cgccacagaa aactcatcat cacgccacac ggcggcgaac attcagacgg cattgccttg
     1741 atagaaaacc tgtggcgcgc ggtcggggcg gaaatttata cgatggacgc gcaacgccac
     1801 gacgcggttt tcgccgccgt ctcccatatg ccccacctga ccgccttcgc ctatgtccac
     1861 cagattctcg accaccccga cggacaggaa tatctgaaat tcgccgccac gggctttcgg
     1921 gacttcaccc gcatcgcctc cggtcatccc gccgtgtggg cggacatctg ccttgccaac
     1981 aaagacagcc tgctgcaact ggttcaaggc ttgggcaaac agttggacgt tttggcaaac
     2041 atcctgacca ccgacgaccg cgaagccctg taccgctatt ttgaagaagc caaaaccaca
     2101 cgcgaccgct ggctggacgg caactgaccg ccctgccgtc tgaaaagcaa accgcccaga
     2161 cccgaaatct gcggcggttt gtctcaaccg ggcctgccgt ccgaacgcgg acgaatcagc
     2221 caaggaatat acttccacgc atacaccaaa agcgcgagtg caaacaaaac cgaagaggtg
     2281 cgtatgctgt gcgtgtaggc agtgccggaa gaaaatacgg caaccatacg gacggcggtt
     2341 gccgccatca tcagccaaaa cgcaacggga acggctttgg gcggcggata aatcggattg
     2401 cccgtatgac cgagcgcggt acgcgccatc atgcccaaag tcagcacgcc gataccgccg
     2461 accccgatca gatgcacacc cagattgagg aaagcgggtt tgaaataaga cgcgccgacc
     2521 gcaatcagcc ccaatccggt aaacagatag ccggcaaaca gaatccacag catcggctct
     2581 ttcaacacgg gtttatacca ccagcggtac acctgcacgg taaagatcac gcctgccgca
     2641 aacgcgaaag ccgccgacag ccaaggcatc acgccgtgcg ccatcagcat ggcggtcagc
     2701 atgggcagcc acagcgaagc ctgcgccacc catttcggac tgggaatctg cggcacattc
     2761 aaccgtttgg acgtaaaaaa cgaaataatc cgcgtaccaa tcagaccgat aaaacccgac
     2821 accatcacca agcccgactg taatccgctc aagagtccgc ctaggttgcc gttgtgcagc
     2881 tggacgtgga acgccgcatg cgtgccgccc aagacgaaca gcgcgaacac ggcaacatag
     2941 ttgcgttgat tctgtgaacg gataacgggc aaagccatgc acaccgcgcc gtaccagaaa
     3001 aacagcgtac cgagtatgcc gcttgccgac gcaccccaac ccgggataaa ggcggcaatc
     3061 cgcgcagcca gccaaaagat agtcaagccg accagaacgc cgccccgcgt gggcggctgc
     3121 cccgtccaag tggcgacggc ggtcagcagg aaggcgacga cgaccagtcc ggcataaccc
     3181 caaatcatct catgcgcgtg ccaatagaaa ccggacagct cgtgcgttcc cgtgtagccg
     3241 aaaccccaca gcaatacgga caatgcgccg tacagagccg ccagcgaata aaacgggcgg
     3301 aacgccattg cccagacggg gtgcttggta aatttcatgt gtgttccttg tttatcatta
     3361 atatatttgc cgcaaaacgg aatcatgccg gcaaaaacgg ttcggcggcg ggaaacagct
     3421 cgcgttcttc aaagcgcgcg tggtcgcgca gggttgtggc aaaagcggta ttccacgccg
     3481 cgttaccgta ttcggggctt gccatcatct gccgcagtcg ggcgtggtct ttctcgaaac
     3541 gttgtttcaa ttcgggggcg acattctgcc aaattggggc aaacttggct tcttcttcgc
     3601 gaaaatgggt ttccaattcg gcaaaatgcg gttcgagttc gtcccgatgc ctttcttccg
     3661 gcgtccgcaa cagacgcacg cacagggaaa gcgaatggtg gtggtcacgc gaaagcccga
     3721 taagggcggg atgtcgtttc aacggtttca tgattttgag ttacaataac aaattatcaa
     3781 acggtagcgc ggatgtttca gacggcatac cgccgcagac aggctgcatt ctaccgcccc
     3841 gacggacagg gtttcaacca ttaaggtatt ttccgtgaaa cttaaaccgt accgataaga
     3901 ataacgatcc ataccgagaa aatcatgtat ttgacacaac atacggacta cgggctgcgc
     3961 gtccttatct acactgccat caacgacgat gcgctggtca acatcagtac catcgccgta
     4021 acctacggca tttccaaaag ccatctgatg aaggtcgtta ccgcgctggt caaaggcggc
     4081 ttcctccaca gtgtgcgcgg aaaaggcggc ggtctgcggc ttgccgcacc gcccgaccgc
     4141 atcaacatcg gctcggttgt ccgccacctc gaaccgatgc agctggtcga gtgcatgggc
     4201 gagaacaacg aatgcctgat tacaccgtcc tgccggctga cgggcatact cggcggcgcg
     4261 atgaagtcat ttttcacgta tctggacggt ttcacgctcc aagacctgct caacaagccg
     4321 acctacgacc tgctctacga accgagaatt ccgattgcgg tgcagtaatg catcaaaatg
     4381 ccgtttcaaa cggatattcc gaccgagcgc gatacgcgcc ggcatttcgg actgccgccg
     4441 gcaaggcgcg caatgccgtc tgaacagccg atgcttcaga cggcatcggt ttccctacaa
     4501 accgaacacc gccgtatcgc cgcaaccttg gcgcaccaat tccgtgccgt cggtcatatc
     4561 gacgacggtg gtcggctcgg ttccgcacca gccgccgtca atcaccaaat cgacggcgtg
     4621 ttccaaacgc tcgcggattt cataaggatc ggtcaatggt tcgccgtctt cgggcagcat
     4681 cagggtgcag cttaaaagcg gctcgcccaa ttcccccagc agggcttgtg caatggcatt
     4741 atcgggaata cgcagcccga tggttttgcg tttcgggtgc agcgtgcgcg ccggcacttc
     4801 cttcgtcgcc tgcaagataa aggtataagg cccgggcgtg gcggctttaa gctgacgaaa
     4861 ctgtacgttg tcaattttgg cgtacgtgcc caactcgctc aaatcggcgc acatcagggt
     4921 caggtggtgt ttcaaatcga ttttgcggat ggagagtatg cgttccatcg ccgccttatc
     4981 gccgagtttg caacccaagg cgtaacacga atcggtcgga taaaccacca cgccgccttg
     5041 attgacgatt tcaaccgcct gcttgatgag gcgttcttgg ggattgtcgg gatgaacggc
     5101 gaaaaactgt gccatggttt gtgtccttat cgtcatgttt tccgtattgt acgcccgaaa
     5161 ttcaaaatcc cgtcattt
//
LOCUS       DAXIHS010000107         5080 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXIHS010000107 DAXIHS010000000
VERSION     DAXIHS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.107"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            200..852
                     /locus_tag="ACFOF2_001846"
                     /pseudo
     CDS             200..852
                     /locus_tag="ACFOF2_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1023..1538)
                     /locus_tag="ACFOF2_001847"
     CDS             complement(1023..1538)
                     /locus_tag="ACFOF2_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108105.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMYIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNVRIQ"
     gene            complement(1832..2356)
                     /locus_tag="ACFOF2_001848"
     CDS             complement(1832..2356)
                     /locus_tag="ACFOF2_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HFC7108106.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLK"
     gene            complement(2894..3178)
                     /locus_tag="ACFOF2_001849"
     CDS             complement(2894..3178)
                     /locus_tag="ACFOF2_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108107.1"
                     /translation="MKLALKRSGKYNVYDPLALDLDGDGIETVATKGFSGSLFDHTNN
                     GIRTATGWIAAYDGFSVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(3175..3468)
                     /locus_tag="ACFOF2_001850"
     CDS             complement(3175..3468)
                     /locus_tag="ACFOF2_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC7108108.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
     gene            complement(3644..3865)
                     /locus_tag="ACFOF2_001851"
     CDS             complement(3644..3865)
                     /locus_tag="ACFOF2_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC7108109.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWKR"
     gene            4888..>5080
                     /locus_tag="ACFOF2_001852"
     CDS             4888..>5080
                     /locus_tag="ACFOF2_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
                     /protein_id="HFC7108110.1"
                     /translation="MAIVSTPHPALSALIILAHYHGIAAKSLGLKAKVVRQPVKRLAM
                     VTLPALVWCDDGNHFKEKWL"
BASE COUNT         1652 a          961 c          776 g         1691 t
ORIGIN      
        1 actttgacaa cacacaagat atacgggaag gacagggcat cagcatatat ttcagcccga
       61 agccgtttca ttcgtttaaa aaactgcttt tttcacaatt tacctttaca aagcctatac
      121 ctatccttac aatttgtttt gctgtttttg gtatattttt ggggctgtac tagattagca
      181 gatatgttac cctcgaaata tgaagataac acactgcaaa ttaaagaaaa aagtacagaa
      241 agaactgctc cgtttttttg cgctggaaat taccgcccgt tctgccgccg atattttggg
      301 tatccatccc aattcggcag cactgttcta ccgtaaaatc cgcacggtta tcaaccatca
      361 tttggccttg gctgccgatg aggtttttga gggccctgtc gagccggacg aaagcgattt
      421 cggcggacgg cgtaaaggca gacgtggtcg cgggcggcag gaaaagtggt tgtcttcggc
      481 attctgaaac gcaacggacg ggtctatacc gttgtcgtaa acaatgccaa gtctgaaacg
      541 ttactccctg tcatcaaaaa gaaaatcatg ccggacagta ttgtttatac cgatagtctg
      601 agcagctgcg acaagttgga cgtgagcggt tttatccatt accgcatcaa ccattccaag
      661 gagtttgcag accgtcaaaa ccacattaac ggcattgaga acttttggaa tcaggcaaaa
      721 cgcgtcttgc gaaaatacaa cggaatcgat tgcaaatctt tcccgctgtt cttgaaagaa
      781 tgcgaatttc gatttaactt cggcacaccg tctcaacagc ttaaaatcct gcgggattgg
      841 tgtggaattt agggctaatc tagtacagcc ccgatttttt tatctctagc cgaatacagt
      901 tcaacccaaa cgtacccgtc ttggatacac cccctcttgt ttcagtggcg taccctgctt
      961 cgcaaatttt acagttatcg cataataatc agccataagt aaccccacat ttctagaaaa
     1021 atttactgaa ttcgtacatt aactactatt cttatagaat tatttatttc atcgtaaagt
     1081 ggaatatatt cactattatc agaaatctca aataaatatt ccccttccgg caaataaaaa
     1141 tcataaatat acatactgta ttttccccca gctccaaata ctccgcctcc taattttcta
     1201 cttctctctg taagcaactc atctataata agttttttac catcttcttc cactttaaat
     1261 attttaaccg atatattata tggatagttt aacttatcat caatccacct ctccccctgt
     1321 tttggatcat aatgtataaa attagttcct aacgatattg cataaggttt accaattttc
     1381 tgtttgagtt caatagatct ttttgttttt ttaccaagag gaaagtctac cacaacaggt
     1441 tctgggctaa tagccatata aaaaatagtt tcaacaaaac tgcagctagc aatcaatagg
     1501 caagatatta ttaataatat ttttctaaat gcagacataa taaaaatttc caaaaaatcc
     1561 atatcagaat atactaatta tttgtataat taataaggaa gagaaaaatt caaaacggct
     1621 atgagatttc cgcctgaaaa taatgaaata aaagtccata gtaatgaata atagctttat
     1681 cagaccccct ttaatgcaaa catagagaat acttcccttg aagcactatc aatttctact
     1741 taactaaaac tctagctcta tttctaaaat tttgtcaaaa atagttaaat acttttattt
     1801 attttaaata atgatatgca tttatggtta tttattttag acagcctaaa acaaacgggg
     1861 aaatcgtgtt ttcccgaaca tggctaatgg atagcatttt gtccgtgagg cggctgtgca
     1921 ggttgtcagc agccaacagc aaatcgcctg cttgcgcggt tgtgccgtct tttttggtgt
     1981 agctgccttg ttgagccaaa gtgttgccgt taccaagatt tttgtttaca tcttttcgag
     2041 aaaattggct ttgcatttgc cttaacaaag atatttctgc tgctcgtgct ttattattta
     2101 tattatattt actctgcatt tttcttacaa cttgtctcaa ctgactttgt gttttttgta
     2161 attcacgttg taccgatttt cctaaacttt catgtttggc tgcataattg gtagaatcag
     2221 ttgacatttg tgtataaaaa tttttacgaa aattttcgta actcagatta attacatccg
     2281 tacttgcagt tataaaatct tgtataaatt caatagaaac tgaattatca tcagatttgg
     2341 ctttgttatt ttccatatta agcctctctt aatttgaaaa aattaacata aacatttaaa
     2401 aataaaacaa aattatataa taaaaaaaat aatgagaata taaaggataa aaagagagtt
     2461 aagttattta atcggacttg ttctctataa atcataaggc accaaataag ccataataaa
     2521 tttagtaaat aaaaactgct tatacccaac tttcttttct tggaacctat tattatttca
     2581 actctggatg aaaaaataga atttaaaaat ttttttcttt gatatatcgt taatatctcc
     2641 ctagtttttc ttaaaaaaaa tataatcata tatatactac acaacattgg taataaataa
     2701 attaaaagcg aaaggtaatt aaattgataa ttgtaaatta tataataaac aacatacgaa
     2761 gaaaaaatta ttatagatga tttactatac gaataaaaaa atatcctacc tagcccgaac
     2821 cttcttataa tttgtactat gtgatcattc atttcaatat ccatttaagc tttcaattaa
     2881 ttttctatat ttttcaatgg catgtatcat ttggttgatg atcaagccat gtatgcaaag
     2941 attggaatat ggtatctgtc gtgctaataa tgcccccgtt actgtttaat ttgcgcacag
     3001 aaaaaccgtc atatgcagca atccagcccg tggcggtgcg gatgccgttg ttggtgtgat
     3061 caaataagct gcctgaaaag cctttggtgg caacggtttc tataccgtcg ccgtccaaat
     3121 ctagggcgag gggatcgtaa acgttatatt tgcctgaacg tttgagagct aatttcaacc
     3181 actctcttgc ccattcaggg aattcaggca gccaatcttc aaatttcgga aattggtccc
     3241 attcttttca agataaggga gaaaatcttg tttataccat ttttccagta actctcccca
     3301 ttcacttatc cctcgtccaa tataatcttc gccccctttc caagggtttg catcatcagg
     3361 aactaaggaa tatataaacc tagatacttc ttctaataca ttgctgtatg tatcaggatc
     3421 cgcaaaaaag tttgctatct tacttggtgt aaaatcatct atattcatta tagattcgcc
     3481 aagcctctcg tttccataat ccttatttaa ttcaattcat gctgtaaaac agtagaggat
     3541 tttagcattt ttctcgtata tctcgagata catttcttaa atttaaaaaa atgttatcga
     3601 gggatgcaag taaagaagca gtatttgtta ttttgttatt ttgttatctc ttccaattac
     3661 tgcttaaaaa gctatctgca gcttgaatag cattattaac agtaatttcc attttatttc
     3721 tattgttatc cacattatag attaaatcag ataaggattg aatagaacca ataaatgaat
     3781 cccaagcttt atatcccaag agtggtgaat agggtgttaa tttcacatct ccaaaaccgt
     3841 taatattaaa atttgcatat gtcatatctg ctttcctttc aagtaaatta aaaaaataac
     3901 gtgaggaatt aaaattaaaa ataaattaat tatcgactca atataatatg aatcaattag
     3961 ctctcttatg gtaaatataa gaggtaaaaa tactaaaaat aaactaataa agttcagaaa
     4021 aatagtaaat ggaatatttt catatggaaa aattctacaa aatacaagta atattccatg
     4081 acatattaac aaatgagtaa aattcattat aaaatatata aatatttccc catcgtaaga
     4141 tatattcaaa taaagacata taaacaatat aaagtatata aaatatattt tttggcatct
     4201 aaaaaataaa cttttagcaa tcatatatga atagcttgta gaatagaaaa cgaaaattat
     4261 taattatcgt aggtgtgcat ttattccaac acggtttcat gcagcttaaa aaccagaaat
     4321 tctttctccc tttatccttt cttaaaaata gcgtttccaa attctgcaac gacaggtttc
     4381 attcaagtag gactgccgtg attgcatcaa ttgcttcgcg gcatggcatc catatcacac
     4441 aacaggacat ttattctcgg gtaagtccac cttcatcctc ttcggccagc aggaacagta
     4501 aaatatgctc tgtactcgga tagtaaatat ttcctttctc actattgttt gtcatttaat
     4561 cgacagtcta ctctttctct aaaatttcgt caataatagt taaatatatt taaatacatt
     4621 taaaataata atatacacct gtttcagtct gtctaaaaca cgggaaaata tgttatttga
     4681 tcacaacggt gactttgggc agcctgtacc ttcaataacg cgtggaaaat agatatatac
     4741 ttatatcgta ttcgaggctg tcttgtttcc ctcaaacaac cattcttcca cacttttgct
     4801 ttacaatttt cgtgtgtatc cttacaatta cccctattgt ttttgtatat tcctttgcca
     4861 accgttattg ctatccaatc taatattatg gctatcgtat ctacaccaca ccccgctctt
     4921 tccgccctca tcatcctcgc ccattatcac ggcattgccg ccaaatcttt gggattgaag
     4981 gcaaaggtag tacgccagcc tgtcaaacgt ttggctatgg tgaccttacc tgcattggta
     5041 tggtgtgatg acggcaacca ttttaaggaa aagtggttgt
//
LOCUS       DAXIHS010000108         4906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXIHS010000108 DAXIHS010000000
VERSION     DAXIHS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.108"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(458..802)
                     /locus_tag="ACFOF2_001853"
     CDS             complement(458..802)
                     /locus_tag="ACFOF2_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108111.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(840..1163)
                     /locus_tag="ACFOF2_001854"
     CDS             complement(840..1163)
                     /locus_tag="ACFOF2_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108112.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYETFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(1166..1450)
                     /locus_tag="ACFOF2_001855"
     CDS             complement(1166..1450)
                     /locus_tag="ACFOF2_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108113.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNVNKIEIHYIENSRTGEKTDFKFKD"
     gene            complement(1433..1759)
                     /locus_tag="ACFOF2_001856"
                     /pseudo
     CDS             complement(1433..1759)
                     /locus_tag="ACFOF2_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1865..2236)
                     /locus_tag="ACFOF2_001857"
     CDS             complement(1865..2236)
                     /locus_tag="ACFOF2_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC7108114.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(2233..2588)
                     /locus_tag="ACFOF2_001858"
                     /pseudo
     CDS             complement(2233..2588)
                     /locus_tag="ACFOF2_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2616..2822)
                     /locus_tag="ACFOF2_001859"
     CDS             complement(2616..2822)
                     /locus_tag="ACFOF2_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108115.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="ACFOF2_001860"
                     /pseudo
     CDS             complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="ACFOF2_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(3774..3986)
                     /locus_tag="ACFOF2_001861"
     CDS             complement(3774..3986)
                     /locus_tag="ACFOF2_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108116.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(4063..4422)
                     /locus_tag="ACFOF2_001862"
     CDS             complement(4063..4422)
                     /locus_tag="ACFOF2_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC7108117.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(4427..>4906)
                     /locus_tag="ACFOF2_001863"
     CDS             complement(4427..>4906)
                     /locus_tag="ACFOF2_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC7108118.1"
                     /translation="KWDWVKNTDYKTPAARPMQTLDGEMAGGNKPPKSITSGGKANAA
                     TYPQLVNQLTGQNLKNIAAQDPRLSLALHKSEKNFPIGTATYEEADRLGKIWVGEGAR
                     QTSGGGWLSIDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
BASE COUNT         1360 a          960 c          777 g         1809 t
ORIGIN      
        1 tcattaatac cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca
       61 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
      121 aatccgacct acggctattt cgtttaagtc ataaatagcc attcatgttc cttctgaatt
      181 tgcttttgct atccttagaa ccaatcatat aaacattatc atcattaatt aatgttctaa
      241 gtttatgaag cggtcaaatc tgtcaaacta ccagagatga agcatatcct cctggaagta
      301 attctgttcg tgctgtttcc atatcttctt tgccatcatc agttacttct ctgtctaaat
      361 aaaatcttac gataagtttt tgtctaattt catcaaattc tgccgtaatt aatcttatat
      421 tgatatgaat acacggttca tacctgctat tagccagtca ggtgtagttg gatggggatt
      481 catttttaat aatatcttca agcaagtgaa atgtaagaaa agcgtagata cttgaaggcc
      541 ggctatctga ttggcaaaaa gaaatttcgg agataaagaa tttccgttta tatttttcat
      601 aaattaaatc aacaccttgt tttacataaa aactaacttc ctcaggaagg atatttataa
      661 cattctctcc ttttactttt atagttaatg gtttgatttt tatgttttca tgaatatctg
      721 ataggattat ttctaaaaaa ttatttttat ttccagtcat tctggctatt ttatgtatat
      781 ttccattgtg ttcgattatc attttatttt ttccaaggat ttccttctat attgatagtt
      841 tatctaaaga tttcatttaa aatatcctga ctaatttctt gtttatataa ttctacaatt
      901 ttctgtttct gtgtatttgt ataaaatttt tcgttggtat ctgcttgata ttttaattgt
      961 ctcaatattt ccatgatttc atattctgat ttttcatcat attcttcaat catgtaatca
     1021 acaaattgtt ttagaatatc aaaggtttcg taactttcca aaattttaat atgtttatcg
     1081 gcaaaattgg acatatacca ttcgtcagtg attggattag tatttaaatc atctaaaaaa
     1141 acatttacat catctaaaaa catgactaat ccttaaactt aaaatctgtt ttctcaccgg
     1201 ttcttgagtt ttcaatgtaa tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc
     1261 aaccgtcttt tgctaaccaa ccatttttag tatcggacat aggaggtatt ctcgcaggag
     1321 ttttgccctg tgggtttgcc ttaacttctt ccattgctaa ttgttccttt aaattattag
     1381 gtattgtacg accggttgat ttttcaacag attgtgtccc tatttttaat gatttgtttc
     1441 ccccagccat ttccccgtct acagtctgca tagggcgggc agcaggtttt ttatagccgg
     1501 tatttttaac ccaatcccat ttagtcgggt taagttctac ttttttaccg cgccaaaccg
     1561 tacctgcggc ctctgctacg gaaagggcag tttgggctgt tgcagttata ttcggatggg
     1621 catcagccca ttgtctggcg gaattaatgc cgttttttac cgcaaaagca ctgtcctgta
     1681 ataggctcgc ggcagcaagt tgtgcttccg gacttaaatt tcctaattca ttaatatctt
     1741 gtaaagtttg ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa atatttgacg
     1801 cgtctatcgt ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg
     1861 gctgctacca accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct
     1921 cgtaatattt tgtattatct aaattaacta ctataatttt aggattacct gatttatctg
     1981 aaatatcaaa acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc
     2041 cagttatttc aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc
     2101 accaaggaaa agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta
     2161 aataactttg cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac
     2221 tattttgttt tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct
     2281 tttgctccac ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat
     2341 agcttctccc caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga
     2401 tgataatcgg tcattaaaat tatgaacatt cgcttccact tttacccctg cttcatgtgc
     2461 tgccataata cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc
     2521 aaccctgtcg ccaacccatg atttttttcg ttcattgcag ctacaagaga atcatagttg
     2581 taatttgcac taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt
     2641 ccagcaaact gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca
     2701 aattctcttg aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca
     2761 aaaacttcat cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc
     2821 atggcttttt cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta
     2881 cctccatcgt cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac
     2941 gcagttctac cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata
     3001 tgccgagcaa aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg
     3061 acatgcttgt tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa
     3121 gacggttgtg tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc
     3181 ccccctgcca tctccccatc tacagtctgc atatggcggg cagcaggttt tttatagccg
     3241 gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc
     3301 gtagttgcgg cctctgctac ggcaagggca gtttgggctg ttgcagttat attcggatgg
     3361 gcatcagccc attgtttggc agagttgata ccgtctttta ccgcaaaagc actgtcttgt
     3421 aatgcggttg cagccgcaag ttgtgcttcg ggacttaaat ttcctaaatg attaatacct
     3481 tgtagagtct gctgcgcggc tgtatctgtg accgggctta ctgcactgtc tgtaattgcc
     3541 ccaatcccta ctccttgaaa acccattaaa aaaggattaa ccgcaccttg gacgatgccg
     3601 cgaacatcat ccatacggtt agcccaccaa tttttattgg ggtcgttttc ccatatcagt
     3661 tttcctgctt catcagcaca gttaggattg gcattgatgc ccacccagtt tcaggcagcc
     3721 tgaaaaggat ttttaatata cggcaaaagg tgggcaacaa gtgcccacct tacctagcta
     3781 acatttatcc caagtatcat atattttttt aagttcttta tccaaagaaa gaactttatt
     3841 ttttcaacac catgaattat ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt
     3901 tctcctacaa ttgcttgaat aatatacaac tcatctttat cgagactaat tttaactgaa
     3961 ctctcttcaa aattaattaa tttcatgaat aattcctttt attttaattc gtcatcaaaa
     4021 taccgtttgc ctgtttttgg tctaaatgct gtcccaccat ctttagtctt ggatatttag
     4081 attataatcg tcaaattccc agtcaaaata ttttgccaaa tttgatatag acttatcttc
     4141 agacgttgaa ttacatattt ccaatatatc atttatcaat tttaatatgt gttcaatatt
     4201 aaaacggttt gtaacaattg agttttttaa tgacatcggt ctatgtatat ttgaaagtaa
     4261 ccattcagtg ctatatatat aaacataaaa ataatcagca ccattacttt catcaaaacc
     4321 tattgataat gtcaaaagta tacaaaaatt ttctaacttg tcttgtggga aatcttccaa
     4381 atcaaagtca gaacaatcat aattttttaa ttttaatctc atataattac ctaatattta
     4441 aatgtccatt tttaatttta ttcctttttc catttgaatc aatagtataa gtttcaaaat
     4501 ttgcttgaat acctgtagtt gcaaattgtg aatttttttc tgttggtggc cgatattgtc
     4561 gagtgccatc tatacttaac catccgcctc cactagtttg tcttgcaccc tcaccaaccc
     4621 aaattttacc tagcctatct gcctcttcat aagttgcagt tcctattgga aaattttttt
     4681 cactcttatg aagagctaga ctcaatcttg gatcttgagc cgcaatgttt tttaagtttt
     4741 gcccagttaa ttgattaact aattgaggat aagttgcagc attggctttt cctccagacg
     4801 ttatagattt aggcggttta ttcccaccgg ccatttcccc atctaaagtc tgcataggtc
     4861 gggcagcagg tgttttataa tcggtatttt taacccaatc ccattt
//
LOCUS       DAXIHS010000109         4837 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXIHS010000109 DAXIHS010000000
VERSION     DAXIHS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.109"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..744
                     /locus_tag="ACFOF2_001864"
     CDS             <1..744
                     /locus_tag="ACFOF2_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC7108119.1"
                     /translation="KLGVKTGLSIDKNRLPYLDEYYKTTKPAGTLFEPDYEALNAYKP
                     QLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDALAQIFGKQAEADKLKA
                     EIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWLHKDIGVPAVDEAIKEG
                     SHGQPVSFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNPLVAETTAWKKGQVVYL
                     VPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            908..1877
                     /locus_tag="ACFOF2_001865"
                     /pseudo
     CDS             908..1877
                     /locus_tag="ACFOF2_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1867..2841
                     /locus_tag="ACFOF2_001866"
     CDS             1867..2841
                     /locus_tag="ACFOF2_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC7108120.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2886..3512
                     /locus_tag="ACFOF2_001867"
     CDS             2886..3512
                     /locus_tag="ACFOF2_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC7108121.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3596..4354
                     /locus_tag="ACFOF2_001868"
     CDS             3596..4354
                     /locus_tag="ACFOF2_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC7108122.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(4413..4751)
                     /locus_tag="ACFOF2_001869"
     CDS             complement(4413..4751)
                     /locus_tag="ACFOF2_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC7108123.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
BASE COUNT          990 a         1459 c         1297 g         1091 t
ORIGIN      
        1 aaattgggcg tgaaaaccgg tttatccatc gataaaaacc ggctgcctta tttggacgaa
       61 tattacaaaa ccacgaaacc tgccggaact ttgttcgagc cggattacga agccttaaac
      121 gcctacaagc cgcagctcat catcatcggc agccgcgccg ccaaggcgtt tgacaaattg
      181 aacgaaatcg cgccgaccat cgaaatgacc gccgataccg ccaacctcaa agaaagtgcc
      241 aaagagcgta tcgacgcgct ggcgcaaatc ttcggcaaac aggcggaagc cgacaagctg
      301 aaggcggaaa tcgacgcgtc ttttgaagcc gcgaaaactg ccgcgcaagg caaaggcaaa
      361 ggtttggtga ttttggtcaa cggcggcaag atgtcggctt tcggcccgtc ttcacgactg
      421 ggcggctggc tgcacaaaga catcggcgtt cccgctgttg acgaagccat caaagaaggc
      481 agccacggcc agcccgtcag ctttgaatac ctgaaagaga aaaatcccga ctggctgttc
      541 gtcctcgacc gaagcgcggc catcggtgaa gagggtcagg cggcgaaaga cgtgttggat
      601 aatccgctgg ttgccgaaac aaccgcttgg aaaaaaggac aagtcgttta ccttgttcct
      661 gaaacttatt tggcggccgg tggcgcgcaa gagctgctga atgcaagcaa acaggttgcc
      721 gacgctttta acgccgcgaa ataatgaaac ggcggcattc gatgccgtct gaaacacgga
      781 tgcaaaccgc ttcctgtgtt tcagacggca ttgcccgata cggaggcttc aaacaaggct
      841 ttccgctccg acggttcgga ctgccttgtt tgaatcttct acgccgtaac gcttttccct
      901 tctgtttatg actgccaaac ctttttccct caacctgact aacctcctgc tgctggcggt
      961 gttgtttgcc gtcagcctgt cggtcggcgt tgccgatttc cgctggtcgg atgtgttttc
     1021 gctgtccgac agccagcagg ttatgttcat cagccgcctg ccgcgcacgt ttgcgattgt
     1081 gctgacgggc gcgtcgatgg cggtggcggg gatgattatg cagattctga tgcgcaaccg
     1141 ttttgtcgaa ccttctatgg cgggcgcgag ccaaagtgcg gctttgggtt tgcttctgat
     1201 gtccctgctg ctgcctgccg cgccgctgcc ggtcaaaatg tcggttgccg ccgttgccgc
     1261 gctaatcgga atgctggtgt ttatgatgct tatccgccgc ctgccgccga cggcgcaact
     1321 gatggtgccc gctggtgggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta
     1381 tcgcgtatga aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcga
     1441 gcgtgctgct ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct
     1501 atctgattgc cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg
     1561 gtttgaaccg gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1621 tggttatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1681 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     1741 ctttggtttt attgtgcgat attttgggac gcgtgattgt gtttccgttt gaaattccgg
     1801 tctcgaccgt cttcggggta ttgggtacgg cgttgttttt gtggctttta ttaaggaaac
     1861 ctgcccatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     1921 gtagcttttg cattgttgct ggtttcctgc gtcctgttta tgacgctcaa cgtcaaaggc
     1981 gattgggatt ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2041 tatgcggtcg gcgtgtccac gcaactcttc caaacgctga ccaacaatcc gattctgacc
     2101 ccttcaattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2161 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaacttgcc
     2221 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2281 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2341 ctgctttcgc gcatgatcga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2401 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2461 gcggcggtcg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2521 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2581 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2641 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     2701 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     2761 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     2821 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     2881 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     2941 aagacccgct tgccgcattc ctcggcgcgg ccgaaaacgg catcctcact taccgctacg
     3001 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3061 ttatcaaagg tctgaaagca ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3121 cctttatgca gggcgcgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3181 tccttaccgg cgccgcaccc gaaaccggct ttggcggcgt aggccctgcc ggacgctttg
     3241 cccgccgcaa cctcttgtcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3301 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgccctgcaa cccttcgcac
     3361 ccgaaatgcg cgaacttatg cccaaaaccg tcggcggcag cgcaagcgca gaagaactcg
     3421 aacgcttcgg acaactctgg caggcacgcg ttaaagcatt tttgaccgaa tcggcggacg
     3481 acccgcagtt cgtcatcgtc cgcgaagtgt gagcgttcag acggcattcc gaatttcaaa
     3541 tgccgtccga acccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3601 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3661 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac
     3721 cctgttttcc tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg cctaccgcgg
     3781 caaaaacctt gcctgcaccc ccaccgccga actcgccaaa accctgtcca tcctcaccca
     3841 agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctcatgttcg gccgctatcc
     3901 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     3961 attccacctg caagacttcg ccgaccgcta cctgaccgag ctttccggcg gacaacgcca
     4021 acgcgccatg attgccatgg tgttctgcca gcgcaccgac tacgttttgc tggacgaacc
     4081 gctgaacaac ctcgacatgt atcatgcccg ctccctcatg caaatcctgc gccggctgac
     4141 cgacgaacac aagcgcacca ccgtcgtcgt attacacgac atcaaccagg ccgccgccta
     4201 cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc atgcagggca agcccgacga
     4261 cattttcacc gccgcaaaca tcaaaaccct gttcgatatg gatgtgtccg tcctcgacca
     4321 ccaaagcaaa aaactcatcg tccaccatat ctgaagctca aaaagaaaat gccgtctgaa
     4381 aacctttcag acggcatttc cattccaaac gctcaaaccg ccgcgtccgc acgttcgccc
     4441 gtgcggatac ggattgcctc ctcgaccggc agcacaaaaa tcttgccgtc gccgattttt
     4501 cccgaacgcg ccacctcgac aatcacgtca atcgcgcgtt ccacagcgtc atccgccaac
     4561 accagctcga ttttgacctt gggcaggaaa tcgacagcgt attccgcgcc ccggtaaatc
     4621 tccgtatgcc ccttctgcct gccgaaccct ttgacctcgc tgacggtcat acccgtaatg
     4681 ccgatttccg tcaacgcctc gcgcacgtcg tcgagtttga acggtttgac aatcgcctcg
     4741 atttttttca tcacattctc cattaattaa cgtaaacaaa cgacaaatat agcagaaacc
     4801 gccggcaaac gataaacggt actgttcgcc cggcgat
//
LOCUS       DAXIHS010000110         4699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXIHS010000110 DAXIHS010000000
VERSION     DAXIHS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.110"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..191
                     /locus_tag="ACFOF2_001870"
     CDS             <1..191
                     /locus_tag="ACFOF2_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108124.1"
                     /translation="VKKERFSLMLHQVWGDVSTHRIAPDARFMRLARSAAGNLPAFPA
                     SGGRNGRHTVYKPQYRLQ"
     gene            224..1144
                     /gene="ribF"
                     /locus_tag="ACFOF2_001871"
     CDS             224..1144
                     /gene="ribF"
                     /locus_tag="ACFOF2_001871"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC7108125.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            1285..4074
                     /gene="ileS"
                     /locus_tag="ACFOF2_001872"
     CDS             1285..4074
                     /gene="ileS"
                     /locus_tag="ACFOF2_001872"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC7108126.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
BASE COUNT         1144 a         1276 c         1253 g         1026 t
ORIGIN      
        1 tggttaaaaa agaacgattt tcactgatgt tgcatcaggt ttggggcgat gtttcaacac
       61 ataggatcgc gcctgatgca cgctttatgc gtttggcgcg ttcggcggcg ggaaatttgc
      121 ctgcttttcc agcgtcgggc gggcgtaacg ggcggcacac tgtctataaa ccgcaatacc
      181 gtttacaatg accgcctgtt tcaccacata cccgaacgca acaatgagaa tcaggctggg
      241 gcggcacaac gcgcccgact ttccgcacgg ggccgccgta accataggca atttcgacgg
      301 cgtacacctt ggacacaaac acatcctcca aaaactccgc ctcgaagccg atacgcgcgg
      361 attacccgtc gtgaccgtcg ttttcgaacc ccaacccaaa gaatttttcg cactccgcac
      421 cggcaggatg ccaccgtgtc ggatcagccc cctgcgcacc aagctcgaat tattggaagg
      481 cacaggctgt gtcgatgccg tctgggtttt gcgtttcgat caaaattttt ccgaaatatc
      541 cgcgcaaggg tttatcgacc gcctgctgcg tcaaaccttg aatacgcgtt atttgctcgt
      601 cggcgatgat ttccgtttcg gtgcggggcg ggaaggctgt tttgaacttt tggcacaaca
      661 gcccgatatg cagaccgagc gtacgccttc cgtcatcgtc gaagacatcc gcaccagcag
      721 taccgccgtg cgacaagccc tttcagacgg caaccttgcc tatgcgaaaa aacttttggg
      781 acacgactac gtcttgagcg gcagggtggt gcacggcaga aaactcggac gcaccttaaa
      841 cgccccgact gccaacatcc gcctgccccg ccaccgttat gcactcggcg gcgtgttcgt
      901 cgtcgaagcg gacggcgcat tcggcacgcg gcgcggcgtg gcgagcttcg gtttcaatcc
      961 caccgttgat agcggctgtt ctcaaaagct tgaagtccac ctgttcgact ttcaaggcga
     1021 cctgtacgga caacggctga acgtccgctt cctgcacaaa ctgcgcgatg aagaaaagtt
     1081 tgacggtatg gcagaactga aaaggcagat agcagccgat atggaagccg caaagtgttg
     1141 gtagaaaaac cttatacaaa ccatccgatt gggctacaat caatctttta actgttcaga
     1201 cggcataggg gtttcccgtt gtgaaatact gtttgaggcg caatgccgtc tgaaccgaaa
     1261 tattgtaaca atagagatta aaaaatgacc gattacagca aaaccgtcaa cctgctcgaa
     1321 agcccgttcc cgatgcgcgg caatcttgcc aagcgcgagc ctgcatggct gaaaagctgg
     1381 tacgagcaaa aacgctacca aaaactgcgc gaaatcgcca aaggccgtcc gaaattcatc
     1441 ctgcacgacg gcccgccgta tgccaacggc gacatccaca tcggtcatgc cgtcaataaa
     1501 attctcaaag acatcattat ccgcagcaaa acccaagccg gttttgacgc gccttatgtg
     1561 ccgggttggg actgccacgg cctgcccatc gaagtgatgg tagaaaaact gcacggcaaa
     1621 gacatgccca aagcgcgttt ccgcgaattg tgccgcgaat atgccgccga acaagttgcc
     1681 cgccagaaaa aagactttat ccgcttgggc gtgttgggcg attgggaccg tccttacctg
     1741 accatggatt tcaaaaccga agccgatacc gtgcgtatgc tcggcgaaat ctacaaatcc
     1801 ggctatctct accggggtgc gaaaccggtt caattctgct tggactgcgg ttcttcgctg
     1861 gccgaagcgg aagtggaata taaagacaaa atctcgcccg cgattgacgt tgcctatccg
     1921 tttaaagaca ccgccgcgct tgccgccgca ttcggtttgg caggtatcga aggcaaagcg
     1981 tttgccgtta tttggacaac cacgccttgg actttgcctg cgagccaagc tgtttcagcg
     2041 ggtgcagacg tggtgtatca attaatcgat acgcccaaag gcaaattggt attggccaaa
     2101 gatttggcgg aagacgcttt gaaacgctac ggcttttcag acggcatttc tattcttgcc
     2161 gaaaccaccg gcgacaagct ggaaaacctg cacatgaacc atccgttcct cgaacgcgat
     2221 attcccatgc ttaacggcga acacgttacc accgatgccg gtaccggctt ggtgcatact
     2281 gcgcccgcgc acggtttgga agactacgcc gtctgcaaca aatacggcat cgagctttac
     2341 aatcctgtca acgccgaagg caaatacatc ggcgaaacgc tgcgcgttgc cggtatgcgc
     2401 gtttgggagg cgaaccccgt catcctgcaa tggttggaag aaaccggcaa ccttttggca
     2461 agcagtaaaa tcgaacacag ctacgcccac tgctggcggc acaaaacgcc gctgatttac
     2521 cgcgcgaccg gtcaatggtt tgtcggtatg gacaaagccg gtgccgacgg caaaaccctg
     2581 cgcgacaaag ccatcaaggc cgtggacgac accgaattct tcccgtcttg gggtcgcgcg
     2641 cgtttggaag ccatgattga aggtcgtcct gactgggtgg tttcacgcca acgctattgg
     2701 ggcacgccga tgactttctt tgttcacaaa gaaacgggcg agctgcatcc gaactctgcc
     2761 gaacttttgg aaaaagttgc cctgaaaatc gaagaaaaag gcatcgaggc ttggttctcc
     2821 ctcgataaaa gcgaattatt aagcgcggaa gattgcgaac attacgacaa actccccgat
     2881 acgatggacg tatggttcga ttccggttcg acccattatt ccgttgtgaa acaacgcgaa
     2941 gaattggaat ggccggcgga tttgtatctc gaaggcagcg accagcaccg gggctggttt
     3001 caatcgtcca tgctgaccgg ctgcgcctca tcaatgggac gcgcaccgta taaacagctg
     3061 ctgacccacg gttttgttgt agacggcgaa ggcaaaaaaa tgtcgaaatc catcggcaac
     3121 gtcgtcgcgc cgcaagaagt ctataacgaa ttcggcgcgg acatcctgcg tctgtgggcg
     3181 gcatctaccg attacagcgg cgaattggcg atttctaaag aaatccttaa gcgcgtaacc
     3241 gaaagctacc gccgtatccg caataccttg agctttttgt ttgccaactt gagcgacttt
     3301 aatccgattg aagatgccgt gcaacaggcg gatatggtgg aaatcgaccg ctatgccctg
     3361 gtgttggcgc gtcggctgca agagcgtctg gcaggcgatt actatccgcg ctacgccttc
     3421 cacttcgccg taaaagacat tgtttctttc tgctcggaag acttgggcgc gttctacctc
     3481 gacatcctga aagaccgcct ctataccacc aaagcagaca gccatgcacg ccgcagcgca
     3541 caaactgccc tgtatcacat cacacgcagt ttggttctct tgattgcacc gattttgtgc
     3601 ttcaccggcg aggaagcgtg ggacatcatc ggcggcggcg aagaagacag cgtccttttc
     3661 catacttggc acgagttccc gaccatcaac gaaaaagccg aagccgaact ggtgaaaaaa
     3721 tggacggcaa tccgcgaagc ccgcgaagcg gtaaccgccg ccattgagcc tttgcgcgcc
     3781 gacaaaaccg ttggttcgtc cttgcaggcg gaagccgaaa tcactgcgcc ggaagaaatg
     3841 gccagctacc tgaacgcttt gggcgaagaa ttgcgctttg ccctgctggt gtctaaagcc
     3901 gaagtgaaag taggtgatga acttgccgtt gccgccaaag ccagcgatgg tgaaaaatgc
     3961 gaacgctgtt ggcactacac ccacgatgtg ggcgcagttg caggccatga aaccatctgc
     4021 aaacgctgcg ccgggaatgt cggcggagaa ggcgaaacgc gccattacgc ctgataaagt
     4081 ttgagcaaat gccgtctgaa accgccaaac agcatttcag acggcatttt ttgtaccgcg
     4141 atttgtcttt ataacggcgg aggggtttca agattgcggt gttgtcggaa tgcaactgaa
     4201 ccgtcattcc cacgaaagtg ggaatctaga atctcgaggt ttcagtcatt tccgatagat
     4261 tcccgcctgt gcgggaatga cggatttcga gattacggtg ttgtcggaac gcaactgaac
     4321 cgtcattccc acggaagtgg gaatctagaa tctcggggtt tcagtcattt ccgatagatt
     4381 cccgccgcgt cggaggtctg gattcccgcc tgtgcgggaa tgacgggttt taagattacg
     4441 gtgttgtcgg acgggtttca agattacggt gttgtcggaa tgcaactgaa ccgccattcc
     4501 cacgaaagtg ggaatctaga atctcgaggt ttcagtcatt tccgatagat tcccgccgcg
     4561 tcgtgggtct ggattcccgc ctgtgcggga atgacggatt tcgagattgc ggtgttgtcg
     4621 gaacgcaact gaaccgtcat tcccacgaaa gtgggaatct agaatctcgg ggtttcagtc
     4681 atttccgata gattcccgc
//
LOCUS       DAXIHS010000111         4675 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXIHS010000111 DAXIHS010000000
VERSION     DAXIHS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.111"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(176..2452)
                     /gene="znuD"
                     /locus_tag="ACFOF2_001873"
                     /gene_synonym="tdfJ"
     CDS             complement(176..2452)
                     /gene="znuD"
                     /locus_tag="ACFOF2_001873"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC7108127.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     NKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFKNKTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGKRPFIAQADQNAPRVPAARLGVHLKASLTDLIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2874..3464
                     /locus_tag="ACFOF2_001874"
     CDS             2874..3464
                     /locus_tag="ACFOF2_001874"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC7108128.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3527..4585
                     /gene="trpD"
                     /locus_tag="ACFOF2_001875"
     CDS             3527..4585
                     /gene="trpD"
                     /locus_tag="ACFOF2_001875"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC7108129.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
BASE COUNT         1045 a         1085 c         1343 g         1202 t
ORIGIN      
        1 ttttgatttt tctgcttttg cgagaatgac ggcgtgaaag caagaatgat gaaacaaaga
       61 aaatgggaat gatggcatag tggtttgttc tttgtctttg ccatatttcc taaaaaattg
      121 attaaaaaag gttttcagaa tgccgtctga aaaccttttt tctgcctgtc cgattttaaa
      181 acttcacgtt cacgccgccg gtaaagctgc ggcccatttg cggcgtatca gagagaaagc
      241 tgctgtgggc gtaaacggat tggttgagca ggttgtcggc tttgacgtac caattccact
      301 cgccatagcg cgtattgcgg cggtagtttg cgccgaggtt gagcatatgg tgtccgggcg
      361 tacgcgtttc gtagcgggcg agtttgtttt gggcgaacac gcggtagtag tccaagttgg
      421 catcgatgag gtcggtcagc gaggctttca ggtgaacgcc gaggcgcgca gccggaacgc
      481 gcggcgcgtt ttggtcggct tgtgcgatga agggacgctt gccgtaagca tcttccctgc
      541 cgggtaggga aggcaggttt ttcagacggc ctcgtacata gtcgccggaa acaccgatgc
      601 ggtagcgcgg tgtcggtttg aagtagattt caccttccgc gccgtagaag tctgcgccgg
      661 attggttgta gcgcacgagc ttcatttcgc tgtcgtcttc gatggatttg gggccgcgtc
      721 cgtcgtttaa ggtttgggcg taaatgtagt tgccgaagcg gttgcggtag agtgccagat
      781 tgtattgcca gcggtcgcct tcgtagccca acgcgagttc gatgttgttg gaacgctctt
      841 tgttcaggtg tttgttgccg acttcaaaag tgttggtggc aacgtgtttg ccgtgtgcgt
      901 acagctcttg cgttgacggc aggcgttcct gatgggaggc ggtcaggctg agtttgtgtt
      961 gcggcgtgaa ataccagttg cccgaaagtg cgaacgagcg ggcggtttgg cggtgcgcgc
     1021 cgaggtcggg cagggggtgg ttgtagtagt tttcccgatc aatcaatgct ttgtcgtacc
     1081 ggatggaggc tttttgtttt tccacgcgta cgccgccttc aagcgtgaag ttgtcccagt
     1141 ttgcctgttc tacaccgaaa aagctgtaat ggcgcacatt gttgtcaatc aacatcggtt
     1201 gtttgaccgc ttcggatgtg gcagataaag cactggattt ttgttgtaaa tattgcacgc
     1261 cccagctgcc tttcagacgg cctatgggtt ggtggcgcaa ctcgatgcgg gcgttttgcg
     1321 ttttgttttt gaagaagttt tccactgcat cgcctgcttt ttcgtcgtgg cggtagtcgt
     1381 tgcggttcaa gtatacgcgc agggcttcaa aaccggggaa cggttgcttc cattcggcac
     1441 ggagttcgta gcgtttgttg cgcaggtcta tccacggtct gccgctgtgg gtgtgtgcgt
     1501 gtgcattatc gtcgtcgtgg aagccgcagc tcaagcccgg attgtcgtaa tcgatgtctt
     1561 cttcggtcaa caggtgcgga taaagctgta aatagcgttt gttaatcaag ctcttttgcc
     1621 agatgatgtc ggcgtggcaa tcatcgtatt cgtggctgtg ggcaggcaga ccatattggt
     1681 cgcgacggtc gctgtacgct acgccgataa aacctttttc gccaacccaa gacagcccga
     1741 tgctgcccgt ttgcgaatcg gcgcggctgt cgggcaggcg tttcagattg cggtaacgcg
     1801 gtacggcgta atcgcccgat ttgcggtaca agccttccgt atgcaataca aagtttttgc
     1861 ccaagccgat attgatgcct gcggatgtca gtttttccaa attgccgctg ctcaaacgca
     1921 atccggcttc gcccgatacg ccgttttcag gcattttttc gggaatttta ttgtcggcaa
     1981 catcaaccag ccccgcgaca ttgcccgaac tatataaaag cgtaaccggg ccgcgcagga
     2041 tttcgacctg ttgcgacaag gcggtatcta ccataatggc gtgatcgggc gaaaaatccg
     2101 ccatatcgcc cgtttcgccg tgatggttca aaaccttaat ccgcctgcct gtttgaccgc
     2161 gtatcaccgg tgcggacgcg ccgccgccgt attgcgaggc gtgaataccc ggtacgccgt
     2221 ccaaagcatc gcctaagttg acggcttttt ggcgcaaggt atcgccggag atgattttgt
     2281 cggaggcggt cgaagtgtgc agcagccccg aagtggcgcg cggacggctt ttgccgacga
     2341 cgctgaccgt ttccaaatcc accgattgct cagtttcatg cgcttgggcg aggaggggtg
     2401 tgttgattaa aagaattgat aaaacaatgg gtttgagtgt agtttgtgcc attttggttt
     2461 ctcgtcgcat ttcaaaagtt tgttattata taacattaca ttccttatat cataagattt
     2521 tgagaacact cagagggcat aggcaaaagt ttttcaaatg aaacggttgc ggcatcgggc
     2581 ggtgtccatt tgtatccgcc gtccttcggg ggcgcggttg atgttgacgc agattccgct
     2641 gtgtttgccc attatgttcg gctgcgggat aaccaaaatt tatgagtgca taaaaacggt
     2701 acgttcccga acggtcgaaa aggtggcaaa atggcgtact tgtcagaacg cgaggctctg
     2761 cgccaggttc acgagggtgc atcgggcacg ctaagatata agagggtatg gatgggggta
     2821 tcggaaatgc agttaataac aaacaaatta taaatcaata ggttaataac aaaatgcttt
     2881 tgtttatcga caattacgac agttttactt acaacatcgt ccagtatttc gctgaattgg
     2941 ggcaggaagt cgccgtgcgc cgcaacgatg atattacgtt ggaagaaatc gaggcattga
     3001 atccgcaata tctcgttatc ggacccggtc cgtgttcgcc caaagaagcg gggatttccg
     3061 tggcggcgat gcgccatttt gccggccggc tgccgattat gggcgtgtgc ctcgggcatc
     3121 agacgatagg cgaggcgttc ggcggcagga tagtccgcgc caaaacgctg atgcacggta
     3181 aggtgtcgcc cgtgtcccat tcgggcaagg gtatgtttaa aggtttgccc aatccggtta
     3241 cctgtacgcg ttatcacagc ctcgttatcg atcgggataa tttgcccgat tgtttggaaa
     3301 tcacggcttg gactgacgac ggcgagatta tgggcgtgcg ccataaggaa tatgccgtcg
     3361 agggcgtgca gttccacccc gaagccctct tgaccgaaca cggacatgat atgttgaaca
     3421 attttttaat cgaatttcaa aacttcaaac cgcaaaaaat ctgacgcgat gccgtctgaa
     3481 ggcttcagac ggcattttcg tccgaatatt gaacggagga caaaaaatga ttacaccgca
     3541 acaggctatc gaacgattaa tcagcaataa cgagttgttt tacgatgaaa tgaccgactt
     3601 gatgcgtcag attatgagag gacaggttct gccggagcag atagcggcca ttttgacagg
     3661 attgcgtatc aaggttgaaa ccgtttccga aattaccgca gctgcagccg tcatgcgcga
     3721 gtttgcgaca aaagtgccgc tggagaatgc agaggggctg gtcgatatcg tcggtacggg
     3781 cggggatggt gccaaaacct tcaatatttc gacgacctcg atgtttgttg ccgcagcggc
     3841 aggcgcgaag gttgccaaac acggcgggcg ttccgtttca tcttccagcg gcgcggcgga
     3901 cgtggtggag cagatgggcg caaacctcaa cctgactccc gaacagattg ctcaaagtat
     3961 cagccagacc ggtatagggt ttatgttcgc ccccaatcac cacagtgcca tgcgccatgt
     4021 cgccccggta cgccgttcgc tcggtttccg aagcattttc aacatattgg gtccgttgac
     4081 caatcctgcg ggcgcgccga accaactttt gggcgtgttc cacaccgatt tgtgcggcat
     4141 tttgtcgcgg gtcttgcaac aacttggttc aaaacacgtt ttggtcgttt gcggggaggg
     4201 cggcttggat gaaattacac tgacgggcaa aacgcgcgtt gccgaactca aagacggaaa
     4261 aatcagcgaa tacgacatcc gcccagaaga tttcggtatc gaaacccgcc gcaatttgga
     4321 tgaaatcaaa gttgccaata ctcaggaatc tttgttgaaa atgaatgagg tgctggatgg
     4381 gaaagaaggg gctgcgcgcg atatcgtatt gcttaatacc gccgccgccc tatatgccgg
     4441 aaatgtcgcc gcttcgcttt cagacggcat atctgccgca cgggaagcca tcgattcagg
     4501 tagggcaaaa tcgaaaaaag aggagtttgt cggttttcaa ccacaacaaa gatgccattt
     4561 tcttggaaag atggagcttg ggtgatgccg ccatgattat ggaacttttg tggcaaaaca
     4621 taagcacttc acgaagagaa cttaccaaac tgtttttata taaaaacttg gggct
//
LOCUS       DAXIHS010000112         4642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXIHS010000112 DAXIHS010000000
VERSION     DAXIHS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.112"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..454)
                     /locus_tag="ACFOF2_001876"
     CDS             complement(<1..454)
                     /locus_tag="ACFOF2_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7108130.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGF"
     gene            complement(451..1869)
                     /locus_tag="ACFOF2_001877"
     CDS             complement(451..1869)
                     /locus_tag="ACFOF2_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC7108131.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(2064..3173)
                     /gene="ribD"
                     /locus_tag="ACFOF2_001878"
     CDS             complement(2064..3173)
                     /gene="ribD"
                     /locus_tag="ACFOF2_001878"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC7108132.1"
                     /translation="MFSDTDISMMEIALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPQLNVRDFPTLRQP
                     ARIVLDSRLRLPPYSHLVTDGQSPTLIATLERDEDKLRPYREHAHIRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            complement(3206..3670)
                     /locus_tag="ACFOF2_001879"
     CDS             complement(3206..3670)
                     /locus_tag="ACFOF2_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC7108133.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAVRFAALHKRFDNPSDFASWLAQAVKTDRKA"
     gene            complement(3699..4541)
                     /locus_tag="ACFOF2_001880"
     CDS             complement(3699..4541)
                     /locus_tag="ACFOF2_001880"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC7108134.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     NDRVAVIATLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          931 a         1168 c         1589 g          954 t
ORIGIN      
        1 ggaaaccgtg gcggatgagt ttttcgcgtt cgtgtttgca gttaccgatg atgtacacgc
       61 ccagcttgtc gaagagtttg gcgattttgg ggtaggttgc cgcgtccaaa ccgtgcgagt
      121 ggtagatgat tttggttttc ggcgaaacga ttttggcggc aatggcgcag gcgggtacga
      181 cgcgcgccat ttggcagtcg atgatgtcgg gttgttcgcg ccgcagcatt cgggcgtagg
      241 caatcatacc tttgaggtag ccgaaaaggc cgccgcgata gaaatcgacg ggttgccaac
      301 gtatgcccaa ggcttgcgcc tctttgagca acggtccgtc cgaagaggca agcatgatgt
      361 cgtgtccgtg ctgcctgagt agcctgccga ggcggacggt ggcggtttcc gtgccgccca
      421 agcctgccat agatgtggtc aaaacgattt tcataatggg aaaccttgtt ttttcaaata
      481 atgaaacagt ttgtgcaaat ctttccggtg gcgcaggatg cagcctgcca gatatgccgc
      541 ccatacgccg gcaaacaggg ggtagtttgc cggtgtgccg aagcaggtgt aggccgccga
      601 ggaggtcagg cagagcaatg tgtgcgtata aagcggcagg cgtttgagcg gctgccacag
      661 gcggcaggag ctttcggtct tgaaggcaaa aaacaaccag aatgaggtgg cacaggcaac
      721 cgccgcgccg cgcgcgccgc cggacggtac ggcaagcccc agcagcagca ggtttgccgc
      781 cagcgcgccc aaggtggcga gcgcgatcgg gcgtgttttt cggacgacgt tcaaaccgat
      841 gccgctgatt tctaccagcg tgcaaaacag cggaggcagc atacacgata cgacgataaa
      901 ccggacggcg gcgtagtttt ccggcagcag gagggaggcg aggggcgaga aaatgccggt
      961 caggcagagg gcggaggcaa gcagggcggc ggcgaattct gccgttgctg agagacgggc
     1021 gggcggggcg ttttcttcga ttgcgcggaa aatatacggt gtccagaccg ttgaaaagat
     1081 gctttggaac aataatgccg ccccgccgaa cgaaataccc atcgaataaa cgccgagctg
     1141 ttctaggccg gcatattttt tcaggaacaa acggtcggcg gatgccagcc cccaataggc
     1201 gatgctgctt agtgcgatcg gtatgccgta gcgcaggccg cgatgcagga cggcggatga
     1261 aaacggtgcg cgccggacgg ccttcagacg gcatcggttt tgaaacagca aaaaggcggc
     1321 ggcggcaagg tttgccagcg cgtaaacggc ggtcaggacg gcggtgttcg ccggaaagtg
     1381 cagcagcccg accgtcagca gcagcagcag gatggcgagc ttggacacga gttgcgagga
     1441 cgaaaaggca agggcgcgtc cttccatacg caaaaccagt aagagaaagc ggatgggcag
     1501 gaagctcagt tcaaacagca ccagcccgat gccggcggcg gcatcgtcga gcgaaaacag
     1561 gatttcagac ggcagggacg ggcgggaaag cagcagggcg gctatcgcgg cggcagacag
     1621 cagcggcggc aggaacaggg ttttgaacaa agtgtctttg tcggcggcgg catagtattc
     1681 gcggacgtat gcctgatcca gcccgaggca caataccgac accgtcagtc ccgccgccgt
     1741 ctgcatcagc acgatgcgcc cgatgtcgtc ggcagggaaa taccacgaca gcagcggcag
     1801 gatgatgacg gctaaaaccg cgctgccgat cgagcctgcc gcgtagccga ggatttcttt
     1861 tgtgtccatt tttgatgtcc gggcggcggc gggatgctgc ctgtgccgtc tgaagccttt
     1921 cttgatcgga atttgacggc tttcagtccg ttgcggctgc cggcggggtg cggcagtccg
     1981 ggttgcgctt ttccgggcgg gcggcggtct gaacggggcc gttttttatc ggcgttatta
     2041 tatagtgaaa cggcgcaaac cctttaaaag gcgttatcgt ttttccggaa cacggttttg
     2101 atgtcgtgtc cgaggatttc ggtggaaacg ggtgtccaca agggcggtgc ggaaagggcg
     2161 gcgcggtttt cgggcaggga aagcaggtct ttgccgctgc cgaggatttt gggcgaacgg
     2221 tacaggacga tttcgtctgc cagattttct gccaaaaatg cggatgtgag ttcggagcct
     2281 gcttcgacca tgatttcgcc gaaaccttcg tcagcaagga ggcgcatcag gtggtgcagg
     2341 tcgattttgc tgtctgccgt ttcagacggc atcaggatac ggatgtgtgc gtgttcccga
     2401 taggggcgga gtttgtcttc atcgcgttcc aaggtggcga taagggtcgg agattgtccg
     2461 tcggtaacca aatggctgta cgggggcagg cgcaggcggc tgtctaacac gatgcgcgcg
     2521 ggttggcgca gggttgggaa gtcgcggacg ttgagttggg gattgtccgc caacaccgtg
     2581 ccgatgccgg tcagcaccgc gcagctttcg gcacgcaaaa cctgtacgtc ggcacgcgcg
     2641 tcttcgccgg taatccaaaa gctgctgccg tctgaaaggg cggttttgcc gtccagcgaa
     2701 acggcgcatt tgaggcggac gaaggggcgg cggcgttcga tgcgcgacag gaagcctcgg
     2761 ttgagttccc ttgcctgatg ttcgagtaaa ccgcattccg tcttgatgcc tgctgcttcg
     2821 agcatggcaa gccctttgcc ggcaaccagc gggttggggt cgcgcatggc ggcaacgacg
     2881 cgtgttacgc cggaacggag cagagcttcg gcgcagggcg gggtacgccc gtaatggctg
     2941 cacggttcga gggtaacaaa ggcggtcgcg ccttgtgcca tttcgcccgc ctgacgcagg
     3001 gcgtggactt cggcgtgcgg ttcgcccgct ttgacgtgga agccttgccc gacaatttgg
     3061 ctgccgtgtg cgataacgca gccgacgcgc ggattgggcg aagtggaaaa acgtcccaaa
     3121 gcggcaagtc ggagggcgat ttccatcatg gatatatctg tgtccgaaaa catagggata
     3181 ccgtatcagt atgggttggg gagaatcagg ctttgcggtc tgttttgacg gcttgcgcca
     3241 gccacgaggc aaagtctgac ggattgtcga agcgtttgtg cagggcggcg aagcggacgg
     3301 cggcttccgt gttgtgctca agcagctctt ttagcaccat gtcggcaagt tcggtggagg
     3361 ggatgtcgcg ctgacccgaa gtgtagagcc tgtgttccgt caggcggacg gtttcgtcga
     3421 tctgttcggg tgtcagggcg gattttcggg cggcggcggt caggtcgttg cggaggcgtt
     3481 gtgcattaaa gggcgagcgt tttttgtccg tgccgatgac ggcgggcatt ttgagttcgg
     3541 cggtttcgag cgtgccgaag cgtttgccgc agttggggca gtggcggcgg cggcgcacgg
     3601 cgttgcgttc ttccatcaga cgcgaatcgg cgacgcgggt gtcggggtgg gcgcaaaacg
     3661 ggcatttcat agcgtttctt cctgatgccg tctgaacgtc aaaccgatac gccggcggcg
     3721 cgggcgattt ccagaccttc ttcggcactc atatagacgg ggttttcggg acggtcgtgt
     3781 cggacgatgt tgccttcgcg gaacatgacc agttcgttca cggcaagttg ggaccatgat
     3841 tcgtcgcggg tcagcggcag ggtggcgata acggcgacgc ggtcgttggg cgtggttact
     3901 tcggcaaaat cgaccatcac gtcgtcgtcg agcaggcgcg ccttgccgaa cggggcttgg
     3961 cggacgatgt agtgcagtag tgtgctggcg tgggcaaaca gggcaatgcc gtctgaaagc
     4021 ataaagttaa acagcccgaa cttgcggatt tcgtgcgtca gccccgcaat cgcgtcaaac
     4081 agcgtgtcgt cgtcgggacg ggcggcaaag cgggtgcgca ggcggttgag gatgtggcag
     4141 aacgcgcgtt cggaatcggt tgtgccgacg gggtggaaaa attcgccctg ttcgggaaaa
     4201 aaatcaatca aatgtccgtt gtgggcaaac agccagtagc cgccccacat ttcgcgcata
     4261 aagggatggg tgttcgccag cgaggtttgt ccttgtgatg ctttgcggat atgggcgacg
     4321 acgttttccg atttaatctg gtaggcgcgc acgaggtcgg cgacgggcga attggcactg
     4381 ggtttgtcgt catggaacag gcgcacgcct ttgccttcga aaaagccgat gccgaaaccg
     4441 tcggcatggt ggtcggtaat gccgcccctg cggcggaagc cttcaaagga aaacataata
     4501 tcggtcggcg tattgcagtt catgcccagc agttgacaca tggtttgtcc caatgattca
     4561 gatggtcgca agtattcgga ttataccccg aactgaaaat gccgtctgaa atacggcttg
     4621 ttccccgtta ttccctcgaa aa
//
LOCUS       DAXIHS010000113         4570 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXIHS010000113 DAXIHS010000000
VERSION     DAXIHS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.113"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(259..1302)
                     /gene="argC"
                     /locus_tag="ACFOF2_001881"
     CDS             complement(259..1302)
                     /gene="argC"
                     /locus_tag="ACFOF2_001881"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC7108135.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSKAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1299..1592)
                     /locus_tag="ACFOF2_001882"
     CDS             complement(1299..1592)
                     /locus_tag="ACFOF2_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108136.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            1846..3000
                     /locus_tag="ACFOF2_001883"
     CDS             1846..3000
                     /locus_tag="ACFOF2_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC7108137.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3009..3236
                     /locus_tag="ACFOF2_001884"
     CDS             3009..3236
                     /locus_tag="ACFOF2_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108138.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            3229..3582
                     /locus_tag="ACFOF2_001885"
     CDS             3229..3582
                     /locus_tag="ACFOF2_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108139.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3643..4269
                     /locus_tag="ACFOF2_001886"
     CDS             3643..4269
                     /locus_tag="ACFOF2_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC7108140.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4173..4421
                     /locus_tag="ACFOF2_001887"
                     /pseudo
     CDS             4173..4421
                     /locus_tag="ACFOF2_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
BASE COUNT         1245 a         1022 c         1156 g         1147 t
ORIGIN      
        1 aactatagtg gattaaattt aaatcaggac aaggcgacga agccgcagac agtacagata
       61 gtacggcaag gcgaggcaac gctgtactgg tttaaattta atccactata gattcccgct
      121 tttgcgggaa tgacggtatt ttagattttt gttttcgcgg aataatgagc caaaagccgt
      181 ccgaacccaa aaaaaccgtc gctttcagac ggcatcaaaa aaccgcaggc acgatgcctg
      241 cggtttgccg tttgcgcttc aggggagcag ggggattgcg cccaagccgt gtgtttcctt
      301 cagtccgaac atgatgttca tattttgcac cgcctgaccc gccgcgcctt tgacgaggtt
      361 gtcgatgacg gaaaggacga cccacacatc ggattgcgcc gcctgttgga tgctgatgcg
      421 gcagaggttt gcgccgcgca cgctgcgggt ttcgggtgtc gaaccggcgg gcaggatgtc
      481 cacgaacagg ctgtcgcggt aatagtcgcg caggatggtt tcggggcaga tgccgtctga
      541 aaggtggagg taaacggtgg cgtgcatacc gcgtatcatc ggcgcgaggt gcggcgtgaa
      601 cacgaatcct tcggcgatgc cgtcctgaag cccggcgatg gtctgcctga tttcgggcag
      661 gtggcggtgt ccagctatgc cgtatgcttt gaagttgtcg ccgacttcgc acaacagcga
      721 accgacattg cctttcctgc ccgcgccgga cacgccggat ttgcagtcgg caatcagcgg
      781 cataccgggc ttcagacggc attgccgcaa cagcggcacg agcggcaggg atacgcaggt
      841 cgggtagcag ccggggttgg cgacgaggcg tgcctgtgcg acggcttcgc ggttgagttc
      901 gctcaatccg tacacggctt gggaaacgag gtcgggggcg gcgtgggtca tgccgtacca
      961 gtgttcccag gtcggaatgt cccgtatgcg gaagtcggcg gaaaggtcga tgacgcgcac
     1021 gccctgttca atcaggcgcg gcgcgtcttt catggcgatg ccgttgggcg tggcgaagaa
     1081 gacgatgtcg cattgttcca aacctgcctc gtcgggcgtt tggaaggcga ggccgtacac
     1141 gccgcgcaaa ctcggaaagt aatcggcaac tgcagttccc gctttgctgc ggctggttac
     1201 ggcggcgact tcgacatcgg gatgggcggc aagcaggcgc agcagttcca cgcccgtgta
     1261 gcccgtcgcg ccgacaatgc cgactttgat ttttttactc atggtttttc ctttgtgtgg
     1321 ttggcgggta tgccgtccga acgctgtccg acggcttgtc cgacggggtt ttgattggcg
     1381 gcggaaggac ggtacacaaa gcgtttgaaa agcgtgtcta atttaatggc atccgcctct
     1441 gtttttaaaa cccagccctg ccgtccggaa taaacataac cgtaggcgcg aaagtcggca
     1501 ggatatttcg gtgcctgccc caaacccaaa ccgtatgcgg cgaaagcggt ggtcgtggag
     1561 aaggaaagca ggaggaggac gagggttttc ataaggttcc aaaccgaacg gggcgatgaa
     1621 gcccgattat agcaaaaggt cgtctgaaaa ccgttttcag acgacctttt atttaattac
     1681 aataaaaatg ttaacaacaa aaaacaaacc gcttttttcc gtttggagac aaatttttaa
     1741 ctaaaattgt caacaattcc ttgacgcaca cacaaaaaaa actttaatat ttgtaaatat
     1801 atgtaacatt ataaatttta aatattttct gagtaagggg aagtaatgag catccatctc
     1861 gacttcggca ttagtcctaa aacgttccga cagacttatc tgtatcaaaa gcccaagctc
     1921 tttaaaggag cggttcggaa tctcgaatcc gcatcttgga aagatatcaa cgagatatac
     1981 caacgagcag acccaaccgc accgctgttt catctgcgta aaaaaggcgc aatcgttcct
     2041 aaagaagaat acgtcgaaag tttcgacgat ttgggcaaaa ctcgctaccg ttttattaaa
     2101 tccgttatct acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac
     2161 gagccgtttt cagaccatat cgcccgtcaa gtcgcccgct ttgccggcgc acatactatt
     2221 gttagtggat atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacccccgc
     2281 gatgtgtatg ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc
     2341 cctatgccat tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc
     2401 gatatggata ttatccttga agcaggtgat gtcctctaca tcccacgcgg ttggtggcac
     2461 agacctatcc cgctcggctg tgaaaccttc cacttcgctg tcggtacctt cccaccaaac
     2521 ggctataatt acctcgagtg gctaatgaag aaatttccca ccatagaaag tctgcgccac
     2581 agtttctcag actgggagca agataggacg cgtatcaacg atactgccac acaaattgct
     2641 gccatgattg ccgaccccgt caattatgaa gccttcagtg aagactttct cggcaaagaa
     2701 cgtaccgata ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca
     2761 gacgacgtca ggttgagatt aaatgccaat aatttggata cgttggaaaa gggatatttg
     2821 attgggaatg ggatgaagat aagcgtagat gagttgggga aaaaagtgtt agaacacatc
     2881 ggtaagaatg aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaatat
     2941 gaagaagtta ggaagttgat ttatcagttg atagagttag attttctgga aattttgtga
     3001 gggattctat gaaaaactgg aagcaattta catttttcgt aatattagta atagcttgtt
     3061 atcaagtgct atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt
     3121 ggaatttgaa ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt
     3181 ttgttagccg aatttttagg aataccaacc aagaaaagga gtataaaaat gattgaactt
     3241 caacttcatg aattgaagct ggtttcaggg ggaggtcctg taacagacaa tatagctgga
     3301 aatgtagcta atgctgccac aaccaaagga ggtcccacat ggggggattt ggttgcaata
     3361 cctgctgccg cagcggcagt ttattatctg ccgaaaaatg cttacggtgc tgctggtgca
     3421 aatggtgtat acaatgtgac tcgtaattgg gtaaatgatg ccgttaatgc acctccttat
     3481 aacggaagac caatctttga gattgaacat ggattaactg ctcccgcaac aaaagcagat
     3541 aaatcaggaa acggctacac tgacggtaca gattactgct gatatttctc atcgtccaga
     3601 caatctctag gggtcgtctg aaactttctt aacttcaatt ttatgaatag acccaagcaa
     3661 cccttcttcc gtcccgaagt cgccgttgcc cgccaaacca gcctgacggg taaagtgatt
     3721 ctgacacgac cgttgtcatt ttccctatgg acgacatttg catcgatatc tgcgttattg
     3781 attatcctgt ttttgatatt tggtaactat acgcgaaaga caacagtgga gggacaaatt
     3841 ttacctgcat cgggcgtaat cagggtgtat gcaccggata cggggacaat tacagcgaaa
     3901 ttcgtggaag atggagaaaa ggttaaggct ggcgacaagc tatttgcgct ttcgacctca
     3961 cgtttcggcg caggaggtag cgtgcagcag cagttgaaaa cggaggcagt tttgaagaaa
     4021 acgttggcag aacaggaact gggtcgtctg aagctgatac acgagaatga aacgcgcagc
     4081 cttaaagcaa ctgtcgaacg tttggaaaac cagaaactcc atattttgca acagatagac
     4141 ggtcagaaaa ggcgcattag acttgcggaa gatcagtggc aaaaacggca ctgggcagac
     4201 aggaattgtc gggattgggc atggtatcct ccgatttggc gaagagcaac gaacctgttt
     4261 atctcgtgaa aataaaaccc gacaaaccaa ccatcactgc atacggtgag gaaaaaccgc
     4321 tgcaaatcgg catgacgttg gaagcagaca tcctacacga gaaacggcga ttgtacgaat
     4381 gggtattgga gctgatttat agtatgtcgg gcaaactgta agacttgagg ctgtaaagat
     4441 aaagaagttg tctgaaacct tttcagacga ccagaagaca atatgagacc tttgcaataa
     4501 cataggttac taaaatttta tgctcaatct cattttcaaa atgcaaaact tttctgattt
     4561 ttcctacttt
//
LOCUS       DAXIHS010000114         4534 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXIHS010000114 DAXIHS010000000
VERSION     DAXIHS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.114"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(135..1073)
                     /locus_tag="ACFOF2_001888"
     CDS             complement(135..1073)
                     /locus_tag="ACFOF2_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC7108141.1"
                     /translation="MLELNGLCKRFGNKTVADDICLTVGRSKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVAHLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRYAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1082..1957)
                     /locus_tag="ACFOF2_001889"
     CDS             complement(1082..1957)
                     /locus_tag="ACFOF2_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC7108142.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWTDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2146..2222)
                     /locus_tag="ACFOF2_001890"
     tRNA            complement(2146..2222)
                     /locus_tag="ACFOF2_001890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2186..2188),aa:Met,seq:cat)
     regulatory      2372..2471
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2564..4465
                     /gene="thiC"
                     /locus_tag="ACFOF2_001891"
     CDS             2564..4465
                     /gene="thiC"
                     /locus_tag="ACFOF2_001891"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC7108143.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1089 a         1291 c         1205 g          949 t
ORIGIN      
        1 gcttttgcgg gaatgacggc agggtggttt ctgttgtttc ggataaattt tgcagccctg
       61 ataaaaaata tggctgcttt ggtaaaaaaa tgccgtctga aaggttttca gacggcattt
      121 gtttttaaga agcatcagcg gaagcggacg atttcccgtt cttcgatatg gatgcgtacc
      181 gtatccttgc ccgataccgc cccggcgtac cgcatatcga ggttcagcca taggatgccg
      241 tgttccggat ggaggacgga caggctgaac gattcgggca aacaggtacg ggataatacg
      301 cggcactcca tgccgtcttg gtcgaaacgc accgcatgtt gcggaatatg gcggtcatcg
      361 tcggtattgg gcagccccat cagatgggcg acctgcacgc cggcaggcgt ttgaaccaag
      421 gtttcgggcg taccgcattg aaggattttc ccctcgtgca tgacggcgat ttcgtctgcc
      481 gccgtgcagg cctcttcggg cgaatgcgtt accaaaacgg cagggatgcc gcccttgcgg
      541 atacgttcgg cagtcatgcg gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac
      601 gattcgtcca acagcagcag ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt
      661 tgcttttcgc ctccggaaag tttttcaggc ttgcggtgcg cctcgttttc cagtccgact
      721 tcggcaagtg ccgccatggc gaggctttcg gcttcggctt tcggcatttt ttgcattttc
      781 aaaccgaatg ccgcattttc cagtgcactc atatggggaa acagcgcgta atcttgaaac
      841 atcagcgaaa tacggcgttt ttcgggcggc atacgggtaa tgttttcccc gttcagccga
      901 atttcgccgc cgtccggacg gataatcccc gcaatcatat tcagcagggt ggattttccg
      961 cagcccgacc gtcccaaaac ggcaagtatt ttgctgcggc cgacagtcag gcagatgtcg
     1021 tcggcgacgg ttttattgcc gaagcgtttg cagagtccgt tcagttcaag catggcgtat
     1081 cctataaacg tatgccgtcc agccactcgg acagcggatg ggcggcatca atcagaccgt
     1141 aacggcaaaa cgcgtccagc gtaaccagtt gcgcgtcgtg catcatgttt tccgacaaca
     1201 tggcatccaa cagaccgccg atgtccatct tttcaaaaca cgcaacctcg ccgtcctgat
     1261 tttcaggcag gaaggtttcg ggcaggatgg catcgaatac atacaggatt tcattgtgca
     1321 caccccggct gacggggcgc aggctgtgca gccgagatac cggacggata aacgggaaca
     1381 gcgttttatc caaaccggct tcttcgctgc tttcgcgaca cacggtttca gacggcaatt
     1441 caccgctgga aacaccgccg gcggcagtat tgtcgagttt gtcgggatcg actgctttgt
     1501 gcggactgcg cctgcctatc cagaaatgcc atcggccgtc cgattcggtc agaccgttga
     1561 gatggacggc gcggctgagt agtccgaacg ggcggaaagc ggcgcgttcg agcgtgaaca
     1621 aggggttgcc gccgccgtcg gtcaggtcga aacactcgtt gcgccagccg tgaagcagcc
     1681 ccgccttgtt ccatgtgcgg gcgaggtgct gcaagcgtcc gcccatatcc gtccagccgt
     1741 ccgcattcag aaaaatgccg tctgaagact ccgagcagcc tgcctcccag tcttttttga
     1801 cgcgctccgc ccattccggc gacagattgc ccaaaggcag acggttcaga tacagcgttt
     1861 tccagcaact ttttgcaccg taacttgctt ttgcccactc gaacagggca tcaaggtctt
     1921 gtttgctgac ggattcggta aaacggacgg tcggcatagt cggctttctt gaacataatg
     1981 cggaagattg tagccaaacc gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt
     2041 ccgatattgc ggcaggaagg aaaatccggc aaaaaacgga ggcggcagga aacaggaaac
     2101 aggcaaaaca aaacccctcg gcattgaacc gaggggttta atatttggtt gcgggagcag
     2161 gattcgaacc tacgaccttc gggttatgag cccgacgagc taccatgctg ctccatcccg
     2221 cgtcagaaga tggaactata cggcagattt tttatgttgt caaacattat ttccgcgaaa
     2281 aatcataaaa ttttgccgtc cggcggttta tgcctgattt gaaatatcat tttctttaca
     2341 aaagttcatg ttcgtgattt aattttggtt aacattgaaa caggggtgct gcatgatgtt
     2401 caggcggctg agaaataccc tttacacccg atctggataa tacctgcgtg gggagttttc
     2461 acggattctg cctttcagac ggcatttttt gatactcggg ccgtttgaaa aagcaaagtg
     2521 ctcctgtttc tttattctaa acgagaaaac aggagcattt tttatgacta cgccaaaaaa
     2581 aactgccaaa acttccggca acgaagcgcg cgaacttgcc gacttgagcg aagacatcgg
     2641 catccgcttc aaatatccga actcggagcg cgtgtatctg caaggcagcc gcgacgacat
     2701 ccgcgtgcct ttgcgcgaaa tccgtcagga cgacacctac acggcgcaag gcacggaagc
     2761 caacccgccg attcccgtct atgacaccag cggcgtgtac ggcgatccgg cggcgcacat
     2821 cgacctgaaa caaggtctgc cgcacatccg cacggcgtgg ctggacgaac gcggtgatac
     2881 cgaaatcctg cccaagcttt ccagcgaata cggcatcgaa cgcgcacacg atccgaaaac
     2941 cgcccatctg cgcttcaacc aaatcacccg cccgcgccgc gccaaagccg gccgcaacgt
     3001 aacccagctc cattacgccc ggcaaggcat tatcacgccg gaaatggaat ttgtcgccat
     3061 acgcgaacgt ttgaaattag acgaattgtc ccaaaaaccg gaatacgcca aactcttgaa
     3121 gcaacacgcg gggcaaagtt tcggcgcgaa catcccgacc cgtcccgatc aaatcacacc
     3181 cgaattcgtg cgccgggaaa tcgccgccgg acgtgcgatt attcccgcca acatcaacca
     3241 tcccgaactc gaaccgatga ttatcggccg caacttccgc gtcaaaatca acggcaactt
     3301 gggcaattcc gccgtcactt ccagcctgac cgaagaagtc gaaaaaatgg tgtggtcgct
     3361 gcgttggggc gcggacacaa ttatggactt atccacaggc gcgcacatcc atgaaacgcg
     3421 cgaatggatt atccgcaacg cgcccgtccc catcggcacc gtgcctattt atcaggcttt
     3481 ggaaaaaacc ggcggcattg ccgaagattt gacttgggat ttgttccgcg acaccttaat
     3541 cgagcaggcg gaacaaggcg tggactattt caccatacac gcgggcgtgt tgctgcgtta
     3601 tgtaccgatg accgccaacc gcctcaccgg catcgtctcg cgcggcggtt cgattatggc
     3661 gaaatggtgt ttggcacatc atcgggaaaa cttcctctac acgcatttcg acgaaatctg
     3721 cgaaatcatg aaagcgtatg atgtgtcgtt cagcctcggc gacggcctgc gccccggctg
     3781 cattgccgat gccaacgacg aatcccaatt cgccgagctg cacaccttgg gcgaattgac
     3841 cgataaagcg tggaaacatg acgtacaagt catgatcgaa ggccccggcc atgtgccgct
     3901 gcaacgcgtc aaagaaaaca tgaccgaaga gctgcaacac tgctttgaag caccttttta
     3961 cacactcggc ccgctcgtta ccgacatcgc acccggctac gaccacatca cctcgggcat
     4021 aggcgcggcc aatatcggct ggtacggcac ggcgatgctt tgttacgtta cccccaaaga
     4081 acatttgggg ctgcccgaca aagaagacgt gcgcaccggc atcatcacct acaaactcgc
     4141 cgcccacgcc gccgatctcg ccaaaggctg gccgggcgca caattacgtg acaacgccct
     4201 gagcaaggcg cgtttcgagt tccgttggcg cgaccaattt cgcttaagcc ttgaccctga
     4261 acgtgccgag agcttccacg acgaaaccct gcctgccgaa ggcgcgaaaa tcgcccactt
     4321 ctgctcaatg tgcggcccca aattctgctc gatgaaaatc acgcaggaag tgcgcgacta
     4381 cgccgacaag caaaaagccc aacggcaggg catggaggaa aaagcggtcg agttcgtcaa
     4441 aaaaggcgcg aagatttaca gttgaaacgt caagcaaaaa atgccgtctg aaacccggaa
     4501 aaaaggcttc agacggcatt ctttcgcttg tgaa
//
LOCUS       DAXIHS010000115         4413 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXIHS010000115 DAXIHS010000000
VERSION     DAXIHS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.115"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            141..518
                     /locus_tag="ACFOF2_001892"
     CDS             141..518
                     /locus_tag="ACFOF2_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC7108144.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            547..792
                     /locus_tag="ACFOF2_001893"
     CDS             547..792
                     /locus_tag="ACFOF2_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108145.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            1026..1994
                     /locus_tag="ACFOF2_001894"
     CDS             1026..1994
                     /locus_tag="ACFOF2_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108146.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            1991..2473
                     /locus_tag="ACFOF2_001895"
     CDS             1991..2473
                     /locus_tag="ACFOF2_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108147.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            2563..3066
                     /locus_tag="ACFOF2_001896"
     CDS             2563..3066
                     /locus_tag="ACFOF2_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC7108148.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            3175..3507
                     /locus_tag="ACFOF2_001897"
     CDS             3175..3507
                     /locus_tag="ACFOF2_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108149.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAAVPQLAEVKKS
                     ANQEGGRQ"
     gene            3504..3914
                     /locus_tag="ACFOF2_001898"
     CDS             3504..3914
                     /locus_tag="ACFOF2_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC7108150.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            3919..>4413
                     /locus_tag="ACFOF2_001899"
     CDS             3919..>4413
                     /locus_tag="ACFOF2_001899"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC7108151.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAP"
BASE COUNT         1338 a          937 c         1023 g         1115 t
ORIGIN      
        1 tagtttttca acagtatagc catagatagc cctttcccat tgtgataaaa tagtgaacta
       61 tttatgcact agaaattaat tagtgcatta ccaatgcgtg aattatgaga ttttaaaatc
      121 tcttttacaa ggattatttt gtgatttcta aatctctttt tggtaatcgt ttgaaagaaa
      181 aaagaaagtt tttagggctt acccaagcac aagctgctga aaaagcaggt attgagcgcg
      241 aaacatgggg caaatacgaa cgtggcgtat ttatgcctag tggagatgta ttgctctctt
      301 ttttgaatat gggaatagat gtgagcagct tatttgctgc tgaacaggga attaggccgt
      361 ctgaaaattc tgaaattagt aaggaagagt cagagttatt agggtattac cgccaagcca
      421 gcgataacgg caaatttgtc attctcagtg tggcaaaagg cgcagagaaa aaagcagccg
      481 aaaatactca agtttcagcc acgcataaaa cagcgtgatt tttaaatcca aaatggagac
      541 agtcttatgt ttgactttga acaacaaatc aaatggggtg agcgagcgga agaaatcgtt
      601 aaagaggcag ccacccagaa caatatcgaa attcccgaac ctttggcttc ggcattagct
      661 aaggcggtaa aagtgcatta tctaagccaa gctggtgtgt tttctttggt agaagcctat
      721 gcagacacgg taaatccaac tgaaaaagaa gtagattacc aagcaatcgg caaagagcta
      781 tttgaaaaat aaaaaatgcc gtctgaaatt ttcagacggc atttaggaca ctccgacagt
      841 cgggctgctc cggtgttctg aaacggatat taagcaattg aagcggccgt gcattttaaa
      901 acgcattaaa aactaccccg acatacaggt catcgggcag gtggttcagg tgttgaaaga
      961 tttgaattga tgccgtctga atttattttt tcgacacaga tattaaaaga gaaaggactg
     1021 tacaaatggc aaaaaaaact actaaaacta ttcattattt acgtgcgcag caaaaccata
     1081 gcttgttcga tttagaggta gcattaagaa gggttttgtc agctgctcct actgttcaag
     1141 atacacaaat tgaacaatac aatcagctta cccaaatcat gcatagaact ttggatccta
     1201 aagatgtaaa aggcaattca attggtggat tactgataca cattggcagc ggtacaaaag
     1261 atgaacatat ccgcactatg agcaatagac ctgtacagca agatgatcat ggtggaacac
     1321 aagctcctcc aagtggatat tcctttttgc gtaaagaggc ttttttgtat attgttggac
     1381 accatgtaat tttttgcgga catggttttt tgtcagcatc aacggttgct tcttatttaa
     1441 gtttacttag caataaattg agagagaaca atccggatat tgttcaattt aatattgaat
     1501 tcaaagcagt aggcaattgc gacaagttgt cattgataca acaacatggg gtaaaaagta
     1561 ttcttttaga tgcttcggca tatcaattat ccagaaacag actgtatcaa gatagccgtt
     1621 caaccattgc taaagcatta gggaaagttg gtagtgtatt tacaagcgaa ttgagtgatg
     1681 aggaattgga agcacaatcc gaaattcata tcaatttaga agtcttgcta aacggtaatt
     1741 cgagagccag tattgaagcg caatcgctga tgcaggagca ggcagaggaa attattgatg
     1801 atgaaacggt taatcaagga ttttccatta ccacgcaaca aggtgaagtg attcagccct
     1861 ctgatgtaaa actgtctaaa tctgttagaa taggacgata cgatgaagcc aactcgttac
     1921 tccctgactc ggcatttaca gcaatcagtg aatatttcct agaactccaa agcagaaact
     1981 taactgaaca atgaaaaaat atctgccctt actacgtttt ttgttgattg cttgtatatc
     2041 agcattttta gcatggaaag ggcaaccttt tgttcatggg aacgagaagg ccgttgattt
     2101 aattattaac gtatttgcaa tcttagccgg ttttttaatt gccattatga cattgtttag
     2161 tgatatgagg tttgatgaag atgcaaattg gcgacaaatt caaattcgtg aaggcgtaca
     2221 agaacaacgg tatataaaac attccttact tttttacaca tatcttgccg tattagtttg
     2281 tgtttttatt gtcatcttgt tggctcataa agaagaatac aaaaatggac cagccatttt
     2341 ttggcttgaa cggagctatt tatttttagc ctgtatttcc attttttatt ctgtattctt
     2401 acctgggaat ttaattaaaa gccgaaaaga agagtttaaa aaattaatgg aaaagaagaa
     2461 acccaaaatc tagaacgagt tttaaccccc tttaaaatcc cattcagacg accttttcta
     2521 aaatccctgt attgattttt aattcaatac agggattttt ccatgtcaga caagttcaac
     2581 caattcatca accgcgtcct ctctcacgag ggtggttacg ccaaccatcc caaagacccc
     2641 ggcggcgaaa ccaattgggg catcactaag cgcaccgcac aggcaaacgg ctacaacggc
     2701 tccatgcgtg ccatgacgcg tgaacaggca atcagcattt accgtaaagc gttttgggag
     2761 cgttaccgcg ccgaccaaat gcccgaagcg gtcgcttttc aattttttga tgcctgcgtc
     2821 aaccacggtt acggcaatgc cgcccgtatg ctccaacgcg ccgcaggcgt gccggacgat
     2881 ggaatcatcg gcgaaatcag cctcaaagcc atcaattccc ttcccgaaaa cgacctttta
     2941 ttgcggttca acgccgagcg tctggtcttt tataccaagc tgggtacgtt cacctctttc
     3001 ggcaagggct gggtacgccg tgtggcgcaa aacctgattc acgccgccgc agacaatgcc
     3061 gattgaggag caaaccatgt caaaaaagtc cattctcgcc ctgatggccg cagccatgca
     3121 gcccgatttc agccacagcg acctaggcat tcgctacgcc atgccgactc agggatgttg
     3181 gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa cgcgcagcca aaaaaaagcg
     3241 tcacaaataa ccgccttttt ccgatggctg ggcggcttgg tctctaatcc ggccacaggg
     3301 aaaatcagcc ataccaaact ttgggcaaac gtcgccgcag ccgccatgac ttggaagttc
     3361 gtgcaggcgg cggacgcgcc cgaatggctt tggtgggcat acggcgcgat ggtcggcggg
     3421 tatgcattaa tcaaacgcgg catcgccgcc gtaccgcagt tggcggaagt caaaaaatcc
     3481 gcaaatcagg aaggggggcg gcaatgattg aatttgtccg agccaaaaaa cggctgcttt
     3541 gggcatttgt gcttttgctt gtgtggacgt gcggttaccg atacgccgcc gacaaggccg
     3601 aagcggaaca aaccgccctg attgccacct atcggcattc ttctatggtt gcggcggaac
     3661 aatatgcctt gcagcttaaa aaagcgcagg acgaaaggca gcggtggtac gacttttccc
     3721 aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc gcaaacgaaa aaagccggct
     3781 atctgaaaac caaggaagaa ctgcttgcgg aattggcttg ccttacagcg gaaatggctg
     3841 ccctaaaaaa gcccgatgcc ttaatccatg ggaaagaagt gcggcagaaa gaacgcaact
     3901 cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt tggagattgt cggactgcca
     3961 cgcggcacct tctattacca attggccgtc caatcggcag aagacaaata tgccgatttg
     4021 aaacggcata tccatgatat ttatcgacga cataagggaa gatacggcta ccggaggatt
     4081 gcggcagcca tccgtcacgc aggaacaccg gtcaatcaca agaaagtcag ccgtctgatg
     4141 gcgaagacgg ggctgaaggc agtgatacgg cggcgcaaat accgctcgtt caaaggagaa
     4201 gtcggcaaaa ttgcgccgaa tatcctgcga cgctgtttcc atgcagaaaa gccgaatgag
     4261 aaatgggtaa cggacgttac cgagttcaat gtaggcggag aaaagatata cctttctccg
     4321 attatggatt tgtttaacgg ggaaatcgtc agttaccgta ttcaaatccg cccgactttc
     4381 gatttggccg gcgagatact gaaaggtgcg ccg
//
LOCUS       DAXIHS010000116         4129 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXIHS010000116 DAXIHS010000000
VERSION     DAXIHS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.116"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(163..1566)
                     /locus_tag="ACFOF2_001900"
     CDS             complement(163..1566)
                     /locus_tag="ACFOF2_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC7108152.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(1635..3062)
                     /locus_tag="ACFOF2_001901"
     CDS             complement(1635..3062)
                     /locus_tag="ACFOF2_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC7108153.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3298..3651
                     /locus_tag="ACFOF2_001902"
     CDS             3298..3651
                     /locus_tag="ACFOF2_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108154.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3642..>4129
                     /locus_tag="ACFOF2_001903"
     CDS             3642..>4129
                     /locus_tag="ACFOF2_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108155.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHPARYAVTFENNVDSKLRRHWVAG
                     ATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKERLTSSEPLSDFVL
                     SV"
BASE COUNT          968 a         1102 c         1018 g         1041 t
ORIGIN      
        1 tatacatttg aattaattcg cacttaattt aaatgtgttt ttaactgtgc tttatttaaa
       61 ggcaatgaga atgtgaaagt atcggatcaa tcccaaagca gcctgcactt tcgaaacggg
      121 gtgcaggctg ctttgggaat ttcataaccg tttcagcctg ctttattccg caaataccgt
      181 ttccaaccct aacccgctct ctttcaccaa gcgcaaataa gccagcatga atttataccg
      241 tgcttgagcc agtttctgtt ctgcttgggc gacttcctgc cgcgcccgta ttacttccag
      301 ccggttgcgg atgccgtatt gttggccggt ttcggtcgat ttcagtttca aacggctgct
      361 ttccaaaacc cgttcttgcg ccatgatttg gtaacgcgcc gcaccgcttt cggtataagc
      421 ctggcgtacg gcgagtttga tgtgccgctc ggttgcggtc agctgtgctt cggcggcccc
      481 gtattgcgct tcggcttcat ggattttgcc cgacaattct ccgccggtat aaagcggcaa
      541 attcaactgt acgccgacgc tcatcccttt gccccgatag tggtagtcat tattctgcgc
      601 agatgaagtg tagaggttat tctgatagcc gacatgggca gaaacggtgg gatagcggct
      661 gttctgtgct gcccgaagcg cctgtccgct gctttgcagg gcaagctgct gcatccggta
      721 ttcatgattg ttggataagg caatgcgctg ccattcatcc agactgtaac gttccagctt
      781 gggcagatag cgtgccaaca ggttggcggt atctatggcc tcgatttgtt tgctatccag
      841 gtcggtgtag tcgttcaact ggttttcata ggtttgtttc tcagccaata cggcgatttc
      901 ttgggccagg gcattgtcgt aaccggcttt ggcttcgtga atatccagcg cggtggcagc
      961 acctttattg aataaagcct gcgcctgcct tacctgctgg gcataagcct ctttttccgc
     1021 cgcatgggcg gcaacggtgt ctcggctgag taaaacgttg aaataacttt cggcaacttt
     1081 caacagcaat tcttcgcgtg ccgcatcgaa acgctgttct gcagcctgcg tatcgaacct
     1141 gctttggcgg tattgtgcaa atttggcagc gtcaaataag gtttgtccca cctgcacgct
     1201 ccatccctgt gtttcgcggg tggaagaaat cgatggcggc tggcgctggt agctggcatt
     1261 ggcggataca tggggaagga atgcggcctt ggcttgttgt tgccgtgcgc gcactgcatc
     1321 acgctggtaa tgggacgctt gaaaatcagc cgaatgttgc tgcgccgccc gccatgcttc
     1381 aggcagcgta aaagccgaaa cggatgggga aagggatagt ggcaaggtaa aaagtgaaac
     1441 gggtaggata tatttggaaa aataggattt catagccgaa aatagttcat gttgcaaata
     1501 gggcgtcagt gtcaggcaaa cggaaatacc gtaatcttgc attatcatta gattgagcaa
     1561 tgtcatccgg gcaatggttt caggcagtct gcatgtccga accggcggat aacaaatgcc
     1621 cagtacggat ccgcctatcg ctccctaaag ctttcgtcca atttggtttg cagcgggctt
     1681 aacagataat ccagcacccg ccgtttaccc gttttaatct ccgccgtgac attcatgccc
     1741 gccgtcagat tcactgcttt gccgtcaata ttcaaggtat gtttgtccag cgacaccacc
     1801 gccgtataaa ccaagcccaa ctgttcgtgg cttaccgcat catggctgac acttttcacc
     1861 ttgcccgtca gataaccgta gcgcgtatag ggaaaactct caatcttcac caccgcatcc
     1921 tgtccctgtt ccacaaaacc gatgtctttg ttcaatacca aaacttccac gtccattttg
     1981 tcgtcatcgg gcgcaaccac catcattttt tgggcagcct gcaccacacc gcccaccgta
     2041 taggtggcca attcctgcac cgtgccgtcc gcaggcgact gtattgtcat cagctgctgc
     2101 cgctgctttg ccttatccgt ttggccgcgg tattggtcaa tctgttcgtt tgcctggcgc
     2161 agcgcatcca gcgtatcgcg tttcaggttc tgcgtattca gcacccgact ctgctccgcc
     2221 tgtgcaatgg ccgcctgaat ctgcctcatc tgaccgcgcg tactttccaa atcgttccaa
     2281 ttgctgaccg atttgctctg ctgctccaaa aacgcatgtt ccgaaataaa attgtcggcc
     2341 cgcaaacggc ggtagtctgc tgttttctgc tgctcgatcg cccccaccga aaccagcttc
     2401 tgctcctgcg ccttggccga ctgcaattcc gcctgatggc cgcgcaaagc cgactgcaat
     2461 tgcgcatcct gcgccgccca tgcctgatac tggtgctgcg ccaacacctg cgccgattgc
     2521 acatcggcat cggagagacc taaagaccgt gcttgcgcca tatcgatatg cggcacggta
     2581 cggctttcca atgccgccaa taccgcttca taacgcagtt tggacaattg ggcagcctgc
     2641 aaagcctgct ccgactgcac cacatcgctg tctgttccca cagcctccag ttccgccagc
     2701 gtttctccct gtttcacatg ctgcccgtcg cgcacatgta ccgccttaac caccgccgtt
     2761 tccagcggct ggatggtttt gctgcgcccg cccgacaccg ttttgcccga agccgccgcc
     2821 acaatatcga ttttgccgaa ccaggaccac aacaaagcca aaagcgcaaa cgccataata
     2881 aaacgcgccg cccatttcgg agcggcagag accggcgtat cggtcagttc caaatgcgcg
     2941 ggcaaaaacg cctgttcttc cgccgtgcgt ttgggcggtt ccaactggtc gcgcaccgcc
     3001 caaacattgc gccatacagt aatgtatcga gaaagaaagg atttcagggc ggagaaaaac
     3061 ataacgggta taaccttggc aatatagaaa caggaaacaa tataaatatg taaaggaatt
     3121 ttaacggaaa gcgcggcagc tgttaaggga aaggcgggaa tattgacaaa aaatacccaa
     3181 gtcgttacaa atattcatta ttttactgcg taacgcaacg ctgaagcgca ggctgctttt
     3241 gagatgcggc aaggttcggc aaaaagcagc ctgcacattt aaccacagga acaacccatg
     3301 tttaccacaa acgatttacg ccatttccta gaaggtttgg ccatcctatt ctcaatcggc
     3361 tattggggca ccatgctgct gttgctttgg tttctcgtcc gctttgccta taaaaagccc
     3421 aaacggaacc ccggcaaaat cctttcaggc agcctgaccg ccgccgctat cctgatgctg
     3481 tttgtttgga ttattcccaa acaattcggc ccgatcaaag aagaaataca ggcacaagaa
     3541 gagtgggaca gaaaatacaa agaagccgaa gccgtgttta acgaacaatg caaaacggcg
     3601 ggggaaagat ttaccagacg gcggacaatg tggaagggat tatgctgttg aaggtagtac
     3661 ctgagcgtac cgtttcggca gatgcaaaaa ccagagaccc gatgtgggac aatgcggctt
     3721 tacagaccag cgaaggcgta aattttattg ctcgtttcct aggatttttt agcgatgggg
     3781 aataccgcta tgtggatgtc ctgcaaccca accattccga tattattcgg tattcaggta
     3841 aagattttcc gataaatcaa atacttaatc atatacaccc cgcccgttat gcggtaacgt
     3901 tcgaaaacaa tgtcgattcc aagctgcgca ggcactgggt ggcaggtgcg accatacgga
     3961 ttatcgaccg ccaaactgac gaagtgattg ccaagaaaac catctatgtc tttgaaaaag
     4021 gcttggacgg cacgggtggg gcgagaatgc cgtggaagtt tgctatcttg tgcaataaag
     4081 aaagacttac ttcttcagag ccgttatcgg attttgttct tagcgtttt
//
LOCUS       DAXIHS010000117         4098 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXIHS010000117 DAXIHS010000000
VERSION     DAXIHS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.117"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(148..804)
                     /locus_tag="ACFOF2_001904"
     CDS             complement(148..804)
                     /locus_tag="ACFOF2_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC7108156.1"
                     /translation="MKNKSKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQIGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFNLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="ACFOF2_001905"
     CDS             869..1759
                     /locus_tag="ACFOF2_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC7108157.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWVVGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
     gene            2029..3840
                     /gene="typA"
                     /locus_tag="ACFOF2_001906"
     CDS             2029..3840
                     /gene="typA"
                     /locus_tag="ACFOF2_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC7108158.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(3852..>4098)
                     /locus_tag="ACFOF2_001907"
     CDS             complement(3852..>4098)
                     /locus_tag="ACFOF2_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108159.1"
                     /translation="LPKLVRTQKAYSFKRHCSTCCREAEPDTETRRGDIPLEKWRQFL
                     DWVGKTPSETASRSRKARPNKKCRLKFFRRHRVSFKH"
BASE COUNT          977 a         1082 c         1175 g          864 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttaccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca ggttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc tgtatgttcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cccgtgccga tttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggctggat
      541 atgttcggga ataccgtttt tcagataatc cctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgaagcata tcgccgccgg cttttgcgct
      721 ataccacata aagggcaagg acgcggcaag cagccagtgg aaaaggcggg tggggaggtc
      781 ccagactttg cttttgtttt tcataatcgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcattgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggtgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggtttccccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcctgggttg tcggttgcgg
     1201 cttgggtacg gataaggcgg cggtcggaac gcttgccgga attttgacgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggca cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aagggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgcctcacc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgcgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggca gggctgttgg ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg
     1741 gataactgaa agtatgtaag aagatatagt ggattaacaa aaaccagtac atcgttgcct
     1801 cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg
     1861 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac
     1921 cgtctgaaag gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc
     1981 gtggtataat gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc
     2041 cgcaacatcg ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg
     2101 ctgcgccaat ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc
     2161 aacgacctcg aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac
     2221 gaaggctacc acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagtg
     2281 gagcgcgttt tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg
     2341 atgccgcaaa cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc
     2401 gtcatcaata aaatcgacaa accgtccgcc cgtccgagct gggtcatcga ccaaactttc
     2461 gagctgttcg acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttatgct
     2521 tccggtctgt ccggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg
     2581 ctgttcgata ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg
     2641 caactgcaaa tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt
     2701 atcttgaacg gacgtatcaa gcccggtcaa gttgttgccg tcatgaacca cgatcaacaa
     2761 atcgcccaag gccgcatcaa ccagcttttg ggtttcaaag gtttagaacg cgtgccgctt
     2821 gaagaagccg aagccggcga catcgtgatt atttccggta ttgaagacat cggcatcggc
     2881 gtaaccatca ccgacaaaga caaccccaaa ggcctgccga tgttgagcgt ggacgaaccg
     2941 acgctgacca tggactttat ggttaacacc agcccgttgg cgggtacgga aggcaaattc
     3001 gtaaccagcc gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg
     3061 cgcgtggaag acaccgccga tgccgacgtg ttccgcgtat ccggacgcgg cgagctgcac
     3121 ctgaccattt tgttggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg
     3181 cgcgtcgtgt accgcgacat cgacggtcaa aaatgcgaac cttatgaaaa cctgactgtg
     3241 gacgtacccg acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa
     3301 ctgactaata tggaaagcga tggcaacgga cgcacccgcc tcgaatacca tattccagcg
     3361 cgcggcttga tcggtttcca aggcgaattc atgaccctga cgcgcggtgt cgggctgatg
     3421 agccacgtgt tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc
     3481 gtgctggtgt cccaagagca gggcgaggcg gtcgcttacg ccttgtggaa tcttgaagac
     3541 cgcggccgta tgttcgtatc acccaacgac aaaatctacg aaggtatgat tatcggcatc
     3601 cacagccgcg acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc
     3661 cgcgccagcg gcaccgacga agccgtgcgc ctgaccacgc cgatcaagct gacgctggaa
     3721 ggtgcggttg agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt
     3781 ctgcgcaagc gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga
     3841 tgtttactgg attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat
     3901 tttttattcg gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc
     3961 caatcaagaa actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg
     4021 ggttcggctt cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc
     4081 cttacaagct taggaaga
//
LOCUS       DAXIHS010000118         4091 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXIHS010000118 DAXIHS010000000
VERSION     DAXIHS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.118"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            301..1818
                     /locus_tag="ACFOF2_001908"
     CDS             301..1818
                     /locus_tag="ACFOF2_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC7108160.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRLVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1877..3757)
                     /locus_tag="ACFOF2_001909"
     CDS             complement(1877..3757)
                     /locus_tag="ACFOF2_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC7108161.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNRLTDKQRVIISPTILP
                     AQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          855 a          926 c         1334 g          976 t
ORIGIN      
        1 tggattaaca aaaaccggta cggcgttgcc tcgccttagc tcaaagagaa caattctcta
       61 aggtgctgaa gcaccgagtg aatcggtttc gtactatttg tactgtctgc ggcttcgtcg
      121 ccttgtcctg atttttgtta atccgctata aatgtgaaat ccgccctttg aaaatcgggc
      181 ggcggttttt tgcctgcccg ctttcagacg gcaaagccgg ttttcacggg tttctgcctg
      241 tttttcggat ggtttgacgt gcttcggcgg cgtgtttgcc agaaaggtaa atgacagggt
      301 atgttgtatt tcagatacgg ctttttggtt gtttggtgtg cggcaggtgt ttctgccgcc
      361 tatggggcgg atgcgcccgc gattttggat gacaaggcat tgttgcaggt gcaacggttg
      421 gtgtcggata agtgggcgga atcggattgg aaagttgaaa atgatgcccc gcgcgtggtt
      481 gacggggatt ttttgttggc gcatccgaaa atgttggaac atagtttgcg cgacgcgctc
      541 aacggcaatc aggcggattt aatcgcttcg ttggcggatt tgtatgccaa gctgccggat
      601 tatgacgcgg ttttgtacgg cagggcgcgg gctttgctgg cgaaattggc gggaaggccg
      661 gcggaggcgg tggcgcggta tcgggaactg cacggggaaa atgcggcaga cgagcggatt
      721 ttgctggatt tggcggcggc ggagtttgac gatttccgac tgaagtcggc agaaaggcat
      781 tttgcggagg cggcaaaatt ggatttgccg gcaccggttt tggaaaatgt ggggcgtttt
      841 cggaaaaaaa cggaggggct gacgggctgg cgtttttcgg gcggcatcag tccggcggtc
      901 aatagaaatg ccaataatgc cgcgccgcag tattgccggc aaaacggagg ccggcagata
      961 tgcagtgtca gccgggcgga gcgggcggca ggcttgaatt atgaaatcga ggcggaaaaa
     1021 ctgacggcgt tggcagataa tcattatttg ttgttccgtt ccaatatcgg cggcacgagc
     1081 tattatttca gtaaaaaatc agcttatgac gacgggttcg gcagagcgta tttgggttgg
     1141 cagtataaaa atgcacggca gacggcgggg attttgccgt tttatcaggt gcagttgtcg
     1201 ggcagcgacg gctttgatgc gaaaacaaaa cgggtaaaca accgccgcct gccgccgtat
     1261 atgctggcgc acggagtcgg cgtgcagttg tcccatactt accgcccaaa cccgggatgg
     1321 caattttcgg tcgcgctgga acattaccgc caacgctacc gcgaacagga tagggcggaa
     1381 tacaataacg gcaggcagga cgggttttat gtttcgtcgg caaaacgttt gggcgaatcg
     1441 gcaactgtgt tcggcggctg gcagtttgtg cggtttgtgc cgaaacgcga aacggtgggc
     1501 ggcgcggtca ataatgccgc ctaccggcgc aacggtgttt atgccggttg ggcgcaggag
     1561 tggcggcagt tgggcggttt gaacagtcgg gtttctgcgt cttatgcccg ccgcaactat
     1621 aagggcattg cggctttctc gacagaggcg caacgcaacc gcgaatggaa tgtctcgctg
     1681 gctttgagcc acgacaagtt gtcgtacaaa ggtatcgtgc cggcgttgaa ttatcgtttc
     1741 ggcaggacgg aaagtaatgt gccgtatgcg aaacgccgca acagcgaggt gtttgtgtcg
     1801 gcggattggc ggttttgaat ggtgggataa tgccgtccga actttggaaa caggttcgga
     1861 cggcattttt gcgcgttcag gcaagggcgg cggcaaatac gccgcgcagg gcgttggaga
     1921 ggcggatttc ttcggcttct tgcagtgttt tttgtgtgat gtgtgtttcg attacttgat
     1981 ttgtttgcaa atatttttgc ggttcgtcca atacggcttg gcgcattatg ccgtttaaaa
     2041 tgtctaaatc taaagagggc gtaagccatt gtccttggta tttgacgaac acgttgcttc
     2101 tgccgccttc gagcaggatg ccgtctgaat tgaaaaacag gctgtcgaac gcgccttgtg
     2161 tttcggcggt ttgccacgct tggtcgaaga cggtgcggca ggtggttttg aagcggcgca
     2221 ggtagttttg tgcgggcagg atggtcggcg aaatgatgac gcgttgtttg tcggtcagac
     2281 ggtttaaaac ggcgcgggac aggctgatgc cgtctgaagc gagcagggct ttgacgcgga
     2341 acgcgccatc gggcaagtcg gcaatgtatt gtttgatttg attttcgcag ccgtcgggca
     2401 ggggcaggtt gagggcttgg gcggaggttt tcagacggca taggtggcgg tcgagcaggg
     2461 tgcagcgtcc gttttccgcg cgcagggttt caaaaatgcc gaagtcgggg cgcaattcgt
     2521 tgaggaaacg ggctttccag ccgcattcgc gatattcggc ggcggggtcg ctgtcgatga
     2581 cgatgccgga accgacaccg tacacgcccc gatacggcgg attggagtca aacggatgcg
     2641 gcgttgcccc gccttgccct gccgttcggg cttgcgcgtt tgaatccggg ccgccgatgc
     2701 ctgaaacaat gccgtctgaa gccgaaacgg gtttgagcga caaggtgcgg atgacgacgt
     2761 tgaatatgcc ttcaaacccc aacccgcccg cgcaaggttt caggtagccg atgctgcccg
     2821 tataaagccc acgcgcttcg gtttcgagcg attcgataat ctgcatactc atttttttgg
     2881 gcgcgccggt gatgctgccg caggggaagg cggcgcggag gatgtcggcg aacgaggtgt
     2941 ttggcagggc ttgggcttgg atggtgctgg tcatctgcca aacgctgccg aaacgcgaca
     3001 ctttaaacgg ctcgggcacg catactttgc cggtttgggc gattttgccg aggtcgttgc
     3061 gcagcaaatc gacaatcatc acgttttcgg cgcggttttt cgggtcggct tgcaattcgg
     3121 cggcgcggcg ttcgtcttgt ccgtcgccca aaatcggcgc ggtgcctttc atcggttcgg
     3181 tgctgatggt gccgtctgaa gcgatgttga ggaagagttc gggcgagaaa cacagcgtcc
     3241 acgcggattg cccctccgca tcgggcaggt gggacaagac ggcatagggg acgggctggc
     3301 gcaggcggcg gtagaggctg acgggattgc cgtaggcttg caggtgcagg cgggtggtgt
     3361 agttgatttg ataggtgtcg ccgcgccgga tggcttcgtg gatttggcgg atgcggtcga
     3421 ggtaatcggt ttcggatacg gaggattgcg gcgtggaaat gccggcggga aggtcgtctg
     3481 agtgtcgggc aagccagctt gcggcatcgg tgtcggcgca gtcggcaaac cagtgcaggg
     3541 caaggttgcc gccgcgctcg gactcaaccc ccgtcagcgg caaaccgaat tcgtagtctg
     3601 caaacaacac cgaatgcaac cctttttgcc agcccttttg cagcgcgccg tccaaagcat
     3661 cgagttcttc gggaccgaaa aaacggcttt ctacatgatt ttgatagcgt tttgcgcggc
     3721 cgcttacggc atcgtcaaac agggcgaaat agggcatggc aattcggggc aaatgttttg
     3781 attatacgct ctttttacac atattttcag acgattcggc aaatatcggc aaaatgtaat
     3841 tttatgtaga gaaagtgggg ggcgaaggtg taaaattgtg aaaacaaatt ccgatttcca
     3901 acctgaaata caataaggag acctttatga cagaccacca gctgcaaccg tttgaaaacg
     3961 tagaattagg cgaaaagcaa gaccagctcc aagtatagtg gattaacaaa aaccagtaca
     4021 gcgttgcctc gctttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa
     4081 tcggttccgt a
//
LOCUS       DAXIHS010000119         3862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXIHS010000119 DAXIHS010000000
VERSION     DAXIHS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.119"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            346..3552
                     /gene="nalP"
                     /locus_tag="ACFOF2_001910"
     CDS             346..3552
                     /gene="nalP"
                     /locus_tag="ACFOF2_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC7108162.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGDGKDIEASFDDEVVIETEAKPTDIRHVKEIGHIDVVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSA
                     PYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGA
                     VGVDSKFGWGLLDTGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGG
                     SQLQLHGNNTYTGKTVIEGGSLVLYGNNKSDMHVETKGALIYNGAASGGSLNSDGIVY
                     LADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGY
                     LNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGN
                     AARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPG
                     IRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDH
                     NATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWS
                     ENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL
                     MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLA
                     GLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAG
                     LGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1041 a         1045 c         1023 g          753 t
ORIGIN      
        1 gaaccgattc acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact
       61 ggtttttgtt catccactat aactagggaa attcaaatta agttagaatt atccctatga
      121 gaaaaagccg tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt
      181 caaatttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc
      241 cctataataa tataattcaa aatataaaaa cgggttacat ctaaacatta cggaattttt
      301 attccctcgc ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca
      361 accttcccta caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt
      421 tctgcctgct taggcggcgg cggaggcggc acttctgcgc ccgacttcaa tgcaggcggt
      481 accggtatcg gcagcaacag cagggcaaca acagcgaaat cagcagcagt atcttacgcc
      541 ggtatcaaga acgaaatgtg caaagacaga agcatgctct gtgccggtcg ggatgacgtt
      601 gcggttacag acagggatgc caaaatcaat gccccccccc cgaatctgca taccggagac
      661 tttacaaacc caaatgacgc atacaagaat ttgatcaacc tcaaacctgc aattgaagca
      721 ggctatacag gacgcggggt agaggtaggt atcgtcgaca caggcgaatc cgtcggcagc
      781 atatcctttc ccgaactgta tggcagaaaa gaacacggct ataacgaaaa ttacaaaaac
      841 tatacggcgt atatgcggaa ggaagcgcct gaagacggag acggtaaaga cattgaagct
      901 tctttcgacg atgaggtcgt tatagagact gaagcaaagc cgacggatat ccgccacgta
      961 aaagaaatcg gacacatcga tgtggtctcc catattattg gcgggcgttc cgtggacggc
     1021 agacctgcag gcggtattgc gcccgatgcg acgctacaca taatgaatac gcatgatgga
     1081 accaagaacg aaataatgtc tgcagccatc cgcaatgcat gggtcaagct gggcgaacgt
     1141 ggcgtgcgca tcgtcaataa cagttttgga acaacatcga gggcaggcac tgccgaccat
     1201 ttccaaatag ccaattcgga ggagcagtac cgccaagcgt tgctcgccta ttccggcggt
     1261 gataaaacag acgagggtat ccgcctgatg caacagagcg attacggcaa cttgtcctac
     1321 cacatccgta ataaaaacat gcttttcatt ttttcggcaa gcaatgacgc acaagctcag
     1381 cccaacacac tgaccctatt gccattttat gaaaaagacg ctcaaaaagg cattatcaca
     1441 gttgcaggcg tagaccgcag tggagaaaag ttcaatggct ccaaccattg cggaattact
     1501 gccatgtggt gcctgtcggc accctatgaa gcaagcgtcc gtttcacccg tacaaacccg
     1561 attcaaattg ccggaacatc cttttccgca cccatcgtaa ccggcacggc ggctctgctg
     1621 ctgcagaaat acccgtggat gagcaacgac aacctgcgta ccacgctgct gacaacggct
     1681 caggacatcg gtgcagtcgg cgtggacagc aagttcggct ggggactgct ggatacgggt
     1741 aaggccatga acggacccgc gtcctttccg ttcggcgact ttaccgccga tacgaaaggt
     1801 acatccgata ttgcctactc cttccgtaac gacatttcag gcacgggcgg cctgatcaaa
     1861 aaaggcggca gccaactgca actgcacggc aacaacacct atacgggcaa aaccgttatc
     1921 gaaggcggtt cgctggtgtt gtacggcaac aacaaatcgg atatgcacgt cgaaaccaaa
     1981 ggtgcgctga tttataacgg ggcggcatcc ggcggtagcc tgaacagcga cggcattgtc
     2041 tatctggcag ataccgaccg atccggcgca aacgaaaccg tgcacatcaa aggcgatctg
     2101 cagctgggcg gcgaaggtac gctgtacaca cgtttgggca aactgctgaa agtggacggt
     2161 acggcgatga ccggcggcaa gctgtacatg tcggcacgcg gcaaaggggc aggctatctc
     2221 aaccgtaccg gacaacgtgt tcccttcctg agtgccgcca aaatcgggcg ggattattct
     2281 ttcttcacaa acatcgaaac cgacggtggt ctgctggctt ccctcgacag cgtcgaaaaa
     2341 acagcgggca gtgaaggcga cacgctgtcc tattatgtcc gtcgcggcaa tgcggcacgg
     2401 actgcttcgg cagcggcaca ttccgcgccc gccggtctga aacacgccgt agaacagggc
     2461 ggcagcaatc tggaaaacct gatggtcgaa ctggatgcct ccgaatcatc cgcaacaccc
     2521 gagacggttg aaactgcggc cgccgaccgc acagatatgc cgggcatccg cccctacggc
     2581 gcaactttcc gcgcagcggc agccgtacag catgcgaatg ccgccgacgg tgtacgcatc
     2641 ttcaacagtc tcgccgctac cgtctatgcc gacagtaccg ccgcccatgc cgatatgcag
     2701 ggacgccggc tgaaagccgt atcggacggg ttggaccaca acgctacggg tctgcgcgtc
     2761 atcgcgcaaa cccaacagga cggtggaacg tgggaacagg gcggtgttga aggcaaaatg
     2821 cgcggcagta cccaaaccgt cggcattgcc gcgaaaaccg gcgaaaatac gacagcagcc
     2881 gccacactgg gcatgggaca cagcacatgg agcgaaaaca gtgcaaatgc aaaaaccgac
     2941 agcattagtc tgtttgcagg catacggcac gatgcgggcg atatcggcta tctcaaaggc
     3001 ctgttctcct acggacgcta caaaaacagc atcagccgca gcaccggtgc ggacgaacat
     3061 gcggaaggca gcgtcaacgg cacgctgatg cagctgggcg cactgggcgg tgtcaacgtt
     3121 ccgtttgccg caacgggaga tttgacggtc gaaggcggtc tgcgctacga cctgctcaaa
     3181 caggatgcat tcgccgaaaa aggcagtgct ttgggctgga gcggcaacag cctcactgaa
     3241 ggcacactgg tcggactcgc gggtctgaag ctgtcgcaac ccttgagcga taaagccgtc
     3301 ctgtttgcaa cggcgggcgt ggaacgcgac ctgaacggac gcgactacac ggtaacgggc
     3361 ggctttaccg gcgcgactgc agcaaccggc aagacggggg cacgcaatat gccgcacacc
     3421 cgcctggttg ccggtctggg cgcggatgtc gaattcggca acggctggaa cggcttggca
     3481 cgttacagct acgccggttc caaacagtac ggcaaccaca gcggacgagt cggcgtaggc
     3541 taccggttct gacggacagg aagcagacag ccgcaaagat cacggtcttt gcggctgttt
     3601 cttatgaaaa gaaaacccta ttccaattgc ctgcttctat tgtttcaaga cttcttccaa
     3661 agattcggca ttaatcagat gtatagcgga ttaacaaaaa tcaggacaag gcggcgaagc
     3721 cgcggacagt acaaatagta cggaaccgat tcactcggtg cttgagcacc ttagagaatc
     3781 gtttctttga gctaagtcga ggcaacgccg tactggtttt tgttaatccg ctatattccg
     3841 ccatctctaa gatttacagc ga
//
LOCUS       DAXIHS010000120         3858 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXIHS010000120 DAXIHS010000000
VERSION     DAXIHS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.120"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            143..1222
                     /gene="hupA"
                     /locus_tag="ACFOF2_001911"
     CDS             143..1222
                     /gene="hupA"
                     /locus_tag="ACFOF2_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HFC7108163.1"
                     /translation="MKYKALSLLPLAAALAACAGGGVAEPHVPVSIPTATPLTGEVKL
                     SSDNSKIENINTANTETHPPRRTRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKS
                     FIFQTPGGAQYALSSYADPIVPSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSY
                     AEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPPPAFSYGNSTPETALSKGKITYQV
                     WGIRVKDGQFVTSSYTPPKGSSFTGYTNTPVLSFITANFNSNKLAGEIRGNSDYGPSV
                     KIENATISGPSFSGNATSGGKTGNLEGKFFGKFNGSYGNTETSIGGKITFKDDRSLDT
                     VFGGVSYVKKLDETANRDTEHLTKQ"
     gene            1250..3676
                     /gene="hupB"
                     /locus_tag="ACFOF2_001912"
     CDS             1250..3676
                     /gene="hupB"
                     /locus_tag="ACFOF2_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC7108164.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFSKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAKNF
                     ELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSPVW
                     QNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALSP
                     WSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
BASE COUNT         1028 a         1214 c          867 g          749 t
ORIGIN      
        1 ctaaggcgag gcaacgccgt actggttttt gttaatccac tataccgatt tttgcaccga
       61 cccacaaaac cagttgacat catataacat ttaaactgat aatcaaaact attattaaca
      121 catcatcaag attgagtatg cgatgaaata caaagccctc tccttactgc cgcttgctgc
      181 cgcccttgcc gcctgtgccg gggggggggt agccgaaccg cacgtccccg tgtccatccc
      241 caccgccacg ccgctgaccg gtgaggtaaa gttatcaagc gataacagca aaatcgaaaa
      301 catcaacacc gccaacactg aaacacatcc gccgcgccgc accagacgct ccctctatgc
      361 ctccccgcaa aacacatcct ccggtatttc cattcagcag cgggaagtag aaaaaatcta
      421 tttcggtgtt aaatcaccag aaaaatcatt catcttccaa acacccggcg gcgcgcaata
      481 cgccctttca tcctacgccg accccatcgt ccccagctac tcctcccccg atttcaaaat
      541 tcccgaccgc cacgcagggc agcggcttgc cgacggcagc cgcatcttta tctgctgcag
      601 cgactccggg gctacttcgt atgcggaaat taccaaacaa gattatatga aattcggggc
      661 gtggataggt cccaacggcg aaatcgacct cttcgccggc ggcttccccg tcggcaaaac
      721 gccacctcct gcgttctcct acggcaacag cacgcccgaa accgcattgt cgaagggcaa
      781 aatcacttat caggtttggg gcatccgcgt caaagacggg caatttgtta cctcctctta
      841 tacgccgccc aaaggcagct cttttaccgg ctacaccaac acccccgtcc tttcctttat
      901 caccgccaat ttcaacagca acaaactggc aggcgaaatc cgcggcaaca gcgattacgg
      961 accgagtgtg aaaattgaaa acgcaacgat cagcggtccg agcttttccg gcaatgccac
     1021 ctccggcggc aaaaccggca acttggaagg caagtttttt ggaaaattca acggcagcta
     1081 cggcaacact gaaaccagca taggcggcaa aatcactttt aaagacgacc gctccctcga
     1141 taccgtattc ggcggtgtga gttatgtaaa aaaacttgat gaaaccgcca atcgagatac
     1201 cgaacacctt actaaacaat aaaccatctc atccctacaa aggtttctta tgtccatccc
     1261 cttcaaaccc gtattggctg ccgccgccat cgcccaagcg tttcccgcct ttgcggcaga
     1321 ccccgcgccg cagtccgccc aaacgctgaa cgaaatcacc gttaccggca cgcacaaaac
     1381 ccaaaaactc ggcgaagaaa aaatccgccg caaaacctta gacaagctct tggtcaacga
     1441 cgaacacgac ctggtgcgct acgaccccgg catttccgtc gtcgaaggcg gcagggcggg
     1501 ttctaacggc tttaccatac gcggcgtgga caaagaccgc gtcgccatca acgttgacgg
     1561 gctggcgcag gcggaaagcc gctcttccga agccttccaa gaattgttcg gcgcgtacgg
     1621 caacttcaac gccaaccgca acacttccga gccggaaaac ttttccgaag taaccatcac
     1681 caaaggcgcg gactcgctca aatccggcag cggcgcattg ggcggcgcag tcaattacca
     1741 aaccaaatcc gcaagcgatt atgtttccga agacaagccc taccatttgg ggataaaggg
     1801 cggcagcgtc ggcaaaaaca gccaaaagtt cagcagcatc accgccgccg gcaggctctt
     1861 tggtttggat gccttattgg tttatacccg ccgcttcggc aaagaaacca aaaaccgctc
     1921 gaccgagggc gatgtcaaaa ttgaaaacaa gggatatgtt ttcagcccaa cccaatcatt
     1981 cagcaagtac cagacctacg tagccacagg ggttgcgcgc tcccaacccg acccgcaaga
     2041 atgggtaaac aaaagcacct tgttcaagct gggctacaat ttcaacgacc gcaaccgcat
     2101 cggctggatt tttgaagact cccgcaccga ccgttttacc aacgagctgt ctaatttgtg
     2161 gacgggtaca atttactctc ccgcaacggg cgactaccgc caccgccaag acgtgagcta
     2221 ccgccgccgc tccggcgtcg aatacaaaaa cgaattggaa cacggcccgt gggacagcct
     2281 caagctgcgc tacgacaagc agcgcatcga tatgaacact tggacttggg acatcccgaa
     2341 aaattacgat ttgcgcggca tcaacagtga agtttaccat tcgttccggc atatccgcca
     2401 aaacaccgcg caatggactg ccgattttga aaaacaactc gacttttcca aagccgtttg
     2461 ggcggcgcaa tacggcttgg gcggcggcag aggggacaat gccaactcgg attacagcta
     2521 tttcgtaaaa ctgtacgacc ccaaaatctt cacttccaac caagccaaaa tcacgatgct
     2581 gatcgaaagc cggtcgaaat ataaatttgc ctattggaac aatgcgtttc acttgggcgg
     2641 caacgaccgc ttccgcctga atgcgggcat acgctacgac aaaaacagca gcagcgcgaa
     2701 agacgatccg aaatacacca ccgccatccg ggggcagatt ccccatttgg gttcggaacg
     2761 cgcgcacgcg ggcttcagct acggcacggg gttcgactgg cggtttacca agcatctgca
     2821 cttgttggca aaatacagca ccggcttccg cgcaccgact tcggacgaaa cctggctatt
     2881 gttcccgcat cctgactttt accttaaagc aaaccccaac ctgaaggcag aaaccgccaa
     2941 aaacttcgag ctgggacttg ccggcagcgg caaagcagga aactttaaat tctccggttt
     3001 ccaaacccgc taccgtaatt ttatcgaact gacctacatg ggcgtatcct cagacaagcc
     3061 taacagccct aattatgcgc cgatttccga cggtactgca ttggtcagct cgcccgtttg
     3121 gcaaaaccaa aaccgctcct cggcatgggt gaaaggtttg gaatttaacg gcacatggaa
     3181 cctcgacagc atcggtttgc cgcaaggcac gcacgcaggt gtcaatgtca gctacatcaa
     3241 aggcaaggcc aaacaaacca acggacagga aacccctatt aacgcccttt cgccatggag
     3301 tgcggtttac aatctgggct acgacgctcc ttccaaacgt tggggcatca acgcctacgc
     3361 cacgcgcacc gccgccaaaa agccgtccga caccgtccac agcaacgacg acttgaacaa
     3421 cccgtggcct tatgccaaac acagcaaggc ctatacgctg ttcgaccttt ccgcctacct
     3481 caacatcggc aaacaggtta cgctccgcgc cgccgcgtac aacattacca acaagcagta
     3541 ctacacttgg gaatctttac gcagcatccg cgagttcggc acggtcaacc gcgttgacaa
     3601 caaaacccac gccggcatcc aacgctttac ctcgccgggc aggagctaca atttcaccat
     3661 cgaagcgaag ttctaagctc ctgatgtgcc aatgccgtct gaaacccgat gatttgggtt
     3721 tcagacggca ttttttacgg tcgcaccgcc gccccctttc aggcttgtac caagcccaat
     3781 cccgcaactc tccgtcattc ccgcaactct ccgtcattcc cgcgactctc cgtcattccc
     3841 acggaagtgg gaatctag
//
LOCUS       DAXIHS010000121         3724 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXIHS010000121 DAXIHS010000000
VERSION     DAXIHS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.121"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..460)
                     /locus_tag="ACFOF2_001913"
     CDS             complement(<1..460)
                     /locus_tag="ACFOF2_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC7108165.1"
                     /translation="MMHTPSPHNEFIRAIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKATGLPAFNMPFYSGLTKTSTALPRL"
     gene            602..1054
                     /gene="dut"
                     /locus_tag="ACFOF2_001914"
     CDS             602..1054
                     /gene="dut"
                     /locus_tag="ACFOF2_001914"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC7108166.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1131..2318
                     /gene="dapC"
                     /locus_tag="ACFOF2_001915"
     CDS             1131..2318
                     /gene="dapC"
                     /locus_tag="ACFOF2_001915"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC7108167.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2481..3260
                     /gene="yaaA"
                     /locus_tag="ACFOF2_001916"
     CDS             2481..3260
                     /gene="yaaA"
                     /locus_tag="ACFOF2_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC7108168.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3343..3433
                     /locus_tag="ACFOF2_001917"
     tRNA            3343..3433
                     /locus_tag="ACFOF2_001917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3377..3379,aa:Ser,seq:tga)
     gene            3511..3600
                     /locus_tag="ACFOF2_001918"
     tRNA            3511..3600
                     /locus_tag="ACFOF2_001918"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3545..3547,aa:Ser,seq:cga)
BASE COUNT          955 a          888 c          903 g          978 t
ORIGIN      
        1 taaggcgagg caacgctgta ctggtttttg ttaatccact ataaaaaggc atattgaatg
       61 cgggtaaacc ggttgctttc cgtttttgga tttcggagaa tgccatcgcc cagctttcat
      121 cacacataaa aaacagtgcg ggcacggctt ttttcagcgg tatttctttc aggtgcaggg
      181 caagtgccgc ccccatcagg atatgccgcg aattaatcat aaaggttacg gtggcgataa
      241 gcagtatcgg cagaggttcc gcccacaggt tgaccgtggc aaactcggag ccgccggcga
      301 agttcatacc ggtcatcaac aacatttcca gccagctcat gcctttttgt ccaccctgca
      361 taccgagtat taatgcccaa ggcagaagtc cgataagtat gggcgaactt tctttgatgg
      421 cgcgtataaa ttcgttatgc ggggaaggtg tgtgcataat gttcgtcttc ataacgggaa
      481 gggcgggcat tatacactgg caacggattt caaaacaaaa ccgatttgcc gtgtttcagc
      541 gtaaacacgg cttgtgtata atctcccatc tttgaaaccg gccgtatgca ggagcaagac
      601 gatgaatatt gaagtagaaa tgaaagtgtt ggacgagagg atggcggatt ttatccctac
      661 ctatgcgaca gcgggttctg ccggtttgga cttgcgcgcc tgtttggatg aggaagtcgt
      721 cttgcagccg ggggaaacgt ttcttgtgcc gacaggtttg gcaatttatt tggccaatcc
      781 ttcatatgcc gccgttttgc tgccccgttc cggcttgggg cataaacacg gcattgtttt
      841 gggcaatttg gtcggtttga ttgactccga ttatcaaggg gaattgaagg tgtcgttatg
      901 gaacagaagc agcgaaccgt ttgccgtcaa gccgtttgag cgtatcgcgc agatggttat
      961 cgttccaatc gtgcaggcgc gtttcaaacg tgtcgaagaa tttgtcggaa gcagccgtgg
     1021 agaaggcggt ttcggcagta cgggtttgca ttgaatataa aatgccgtct gatgggtgcc
     1081 gtcaggttca gacggcatat cttccgatat accgacaacc ggagaacatt atgaatacct
     1141 tactcaacca actcaaaccc tatccctttg cccgactgcg tgaagcgatg cagggcattt
     1201 ccgcccccga aggtctggaa gccgtcccac tgcatatcgg cgaaccgaaa catccaacac
     1261 cgaaagtcat tacagatgcg ctgaccgcct cattgcacga gttggaaaaa tatccgctga
     1321 cggccggttt gcctgaactg cgtcaggcgt gtgcgaactg gttaaaacgc cgttatgatg
     1381 ggttgacgct gaattccgac accgaaattc tgccggtttt gggcagtagg gaggcgttgt
     1441 tttcttttgt ccaaaccgtg ttgaaccctg tttcagacgg catcaaaccc gtaatcgtca
     1501 gcccgaatcc cttttatcag atttatgaag gtgcgacact tttgggcggc ggtgaaatcc
     1561 attttgccaa ttgccccgcg ccgtctttca accccgattg gcgtagtatt tccgaagagg
     1621 tttggaaacg caccaaactg gtgttcgtct gctcgcccaa caaccccagc ggcagcgtgc
     1681 tggatttgga tggatggaaa gaagtttttg atttacagga taaatatggt ttcattattg
     1741 cctcggatga atgctattcc gaaatctatt tcgacggcaa caaaccttta ggctgcctgc
     1801 aggccgctgc acagttgggt cgaagcaggc aaaaactgct tatgttcacc agtttgtcca
     1861 agcgttccaa cgttccgggc ctgcgttccg gttttgtcgc cggcgatgcc gaactgctta
     1921 aaaactttct gctttacaga acctatcacg gcagtgcaat gagcattccc gtgcagcgcg
     1981 caagcattgc cgcttgggat gacgaacagc acgttatcga caaccgccgc ctgtatcagg
     2041 aaaaatttga gcgcgttatt cccattttgc aacaggtatt tgatgttaaa ttaccagatg
     2101 cctcgtttta catttggttg aaagtccccg atggcgacga tttggcattt gcacgcaatt
     2161 tatggcaaaa agccgctatc caagtattgc ctggacgttt tttggcacgg gataccgaac
     2221 agggcaatcc cggggaaggt tatgtgcgta ttgctttggt tgccgatgtc gcaacttgtg
     2281 tcaaagctgc ggaaaccatt gtttccctat atcggtaaag aataaaaaat accgtctgaa
     2341 cttttgttca gacggcattt ttcaatattt tacggttgaa tttgctataa cggtatttac
     2401 ttcactttta atctaatcgg cttgcccgca aacacgttta aacttaaaat ccccgtgttt
     2461 gacacaatac cgagcagatt atgttttttg tcctttcccc tgcgaaaaac cttaacgaaa
     2521 aagaccctgc ccctgtcagc gagtttaccc aacccgacct gctggcagag tccgaaattc
     2581 taatgcagca gctgcgcgag cttgcgttgc aacagattgc cgaactgatg cacgtttccg
     2641 acaaaattgc cctcttaaac gcgcagcgca atgcagaatg gcacacgccg tttacgccgg
     2701 aaaacgccaa acaggcggtc tttatgttca acggcgatgt ttacgaaggt atggatgcaa
     2761 acacattgga tattggacag atacgctatc tgcaaaacca tgtccgcctg ctgtccggtc
     2821 tatacggtct tcttcgcccg ttagacctga tacagcccta tcgtttggaa atggggacgg
     2881 catttgccaa tttgcgcggc aagaatttgt atgagttttg gggcgacatc attaccaacc
     2941 ttttaaatga cacgcttgcc caagcaggca gcaatacgct tgtcaacctt gcctcacagg
     3001 aatatttcaa gtccgtcaac acgaaaacac ttcgggcgca gctgattacc ccgatattta
     3061 aagacgaaaa aaacggtaaa tataaaatca tcagtttcta tgccaagcgt gcgcgcggat
     3121 taatggtgcg ctatgcggca gaacatcata ttaccgaccc tgaaatgctg aaaaatttta
     3181 attacgaagg ctacgcattc aatgacgcgg cttcaaatga aagcgaatgg gtttttatgc
     3241 gttcggaaca aataaagtga aaacaataaa ttaagtattt tccgaaaaaa gtgcttggca
     3301 aaatgtataa acttcattat tattcttctt cttcaagaag acggaagcgt ggcagagcgg
     3361 tttaatgcaa cggtcttgaa aaccgtcgag ggttgatagc cctccgtgag ttcgaatctc
     3421 accgcttccg ccaatccttg gggtcaaaaa ccaaataaaa atgaagtagc ataaagcatc
     3481 ggcattttta tttggtttta cttcattatc ggaaacgtgg cagagaggct gaatgcagcg
     3541 gactcgaaat ccgctgaggg tgcaaatcct ccgtgggttc gaatcccacc gtttccgcca
     3601 caaaacaaaa ccgccctgat tcggggcggt tcttttttgt tcaagttgag acctttgcaa
     3661 aattcccttc cctcccgaca gccgaaaccc aaacacaggt tttcggctgt tttcgcccca
     3721 aata
//
LOCUS       DAXIHS010000122         3587 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXIHS010000122 DAXIHS010000000
VERSION     DAXIHS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.122"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            975..1298
                     /locus_tag="ACFOF2_001919"
     CDS             975..1298
                     /locus_tag="ACFOF2_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC7108169.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            1412..>3587
                     /locus_tag="ACFOF2_001920"
     CDS             1412..>3587
                     /locus_tag="ACFOF2_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC7108170.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     HTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLAGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          880 a         1219 c          910 g          578 t
ORIGIN      
        1 gagtttctgc gaagctaaaa ccgaagctgc aaaaaatcga aaagcagcct gcacgttgaa
       61 aatgccgccc aagcaaacct gcggtttgcc gccctctccc tagccctctc ccacagggag
      121 aggggattgg ggtgcaggct gcctttaagg tttaggcaaa tttttaactt cgttgaagct
      181 gcgatttcag aagctccgtt ttcagacgac cttttggaac accacaggca cacgcatcga
      241 acggctgaat caaagattca gaccgatggc agtccgcacc cgagtttatg cggcaaacag
      301 cgaggctacg gcaacccgtc ccctctccct gtgggagagg gttagggaga gggcagtaag
      361 ccgtaggctt acatcaaagc cgataacgct ttcacaaagc ctgtgtgcac tgaaaactga
      421 aaggtcgtct gaaaacggag ctttcgaagg aagcagagtt tctgcgaagc taaaaccgaa
      481 gctgcaaaaa atcgaaaagc agcctgcacg ttgaaaatgc cgcccaagca aacctgcggt
      541 ttgccgccct ctccctagcc ctctcccaca gggagagggg attggggtgc aggctgtctt
      601 taagcttcag gcgattttta actcttgttg aagctgcgat ttcagaagct ccgttttagc
      661 ttcgcagaaa ctccgcttcc ttcgaaagct ccgttttcag acgacctttt ggaacaccac
      721 aggcacacgc atcgaacggc tgaatcaaag ttcagaccga tggcagcccg cacccgagtt
      781 tatgcggcaa acagcgaggc tacggcaacc cgtcccctct ccctgtggga gagggttagg
      841 gagagggcgg taagccgcag gcttacatca aagccgataa cgcttccgtt acaactccgc
      901 ccactgaaag cagcctgcaa cgaagccaaa acgacaaacc gcatcgtaaa ccacccaacc
      961 cataggagaa ccccatgcaa aacgaaacca tcaacccgaa acagcacctt gccgccatca
     1021 aagaatactg gcagcccgaa atcatcaacc gccacgggtt ccaattccgc ttggtcaaac
     1081 ttttgggcga ttacggctgg catacgcacg gatacagcga caaagtgctg tttgccgtgg
     1141 agggcgacat ggcggtggac ttcgccgacg gcggcagcat gacgatacgc gagggcgaga
     1201 tggcggtcgt gccgaagtcg gtgtcgcacc gcccgcgttc ggaaaacggc tgctcggtgg
     1261 tgctgattga gttgtccgac ccgtccgagg ccgtctgaaa acgaagtttc cgaaggaagc
     1321 tgagtttctg cgaagctaaa agcagcctgc accttcaatc aatatgccga aaatacaacc
     1381 ccaccgcaca ccaacacaca aaggaaatcc catgacacgc ttcaaatatt ccctgctgtt
     1441 tgccgccctg ttgcccgtgt acgcgcaggc cgatgtttct gtttcagacg accccaaacc
     1501 gcaggaaagc actgaattgc cgaccatcac cgttaccgcc gaccacaccg cgagttccaa
     1561 cgacggctac actgtttccg gcacgcacac cccgctcggg ctgcccatga ccctgcgcga
     1621 aatcccgcag agcgtcagcg tcatcacatc gcaacaaatg cgcgaccaaa acatcaaaac
     1681 gctcgaccgc gccctgttgc aggcgaccgg caccagccgc cagatttacg gctccgaccg
     1741 cgcgggctac aactacctgt tcgcgcgcgg cagccgcatc gccaactacc aaatcaacgg
     1801 catccccgtt gccgacgcgc tggccgatac gggcaatgcc aacaccgccg cctatgagcg
     1861 cgtagaagtc gtgcgcggcg tggcggggct gctggacggc acgggcgagc cttccgccac
     1921 cgtcaatctg gtgcgcaaac gcccgacccg caagccattg tttgaagtcc gcgccgaagc
     1981 gggcaaccgc aaacatttcg ggctgggcgc ggacgtatcg ggcagcctga acaccgaagg
     2041 cacgctgcgc ggccgcctgg tttccacctt cggacgcggc gactcgtggc ggcagcgcga
     2101 acgtagccgc gatgccgaac tctacggcat tttggaatac gacatcgcac cgcaaacccg
     2161 cgtccacgca ggcatggact accagcaggc gaaagaaacc gccgacgcgc cgctcagcta
     2221 cgccgtgtac gacagccaag gttatgccac cgccttcggc ccgaaagaca accccgccac
     2281 aaattgggcg aacagccgcc accgtgcgct caacctgttc gccggcatcg aacaccgctt
     2341 caaccaagac tggaaactca aagccgaata cgactacacc cgcagccgct tccgccagcc
     2401 ctacggcgta gcaggcgtgc tttccatcga ccacaacacc gccgccaccg acctgattcc
     2461 cggttattgg cacgccgacc cgcgcaccca cagcgccagc gtgtcattga tcggcaaata
     2521 ccgcctgttc ggccgcgaac acgatttaat cgcgggtatc aacggttaca aatacgccag
     2581 caacaaatac ggcgaacgca gcatcatccc caacgccatt cccaacgcct acgaattttc
     2641 ccgcacgggt gcctacccgc agcctgcatc gtttgcccaa accatcccgc aatacggcac
     2701 caggcggcaa atcggcggct atctcgccac ccgtttccgc gccgccgaca acctttcgct
     2761 gatactcggc ggcagataca gccgttaccg caccggcagc tacgacagcc gcacacaagg
     2821 catgacctat gtgtccgcca accgtttcac cccctacaca ggcatcgtgt tcgacctgac
     2881 cggcaacctg tctctttacg gctcgtacag cagcctgttc gtcccgcaat cgcaaaaaga
     2941 cgaacacggc agctacctga aacccgtaac cggcaacaat ctggaagccg gcatcaaagg
     3001 cgaatggctt gcaggccgtc tgaacgcatc cgccgccgtg taccgcgccc gtaaaaacaa
     3061 cctcgccacc gcagcaggac gcgacccgag cggcaacacc tactaccgcg ccgccaacca
     3121 agccaaaacc cacggctggg aaatcgaagt cggcggccgc atcacgcccg aatggcagat
     3181 acaggcaggt tacagccaaa gcaaaacccg cgaccaagac ggcagccgcc tgaaccccga
     3241 cagcgtaccc gaacgcagct tcaaactctt cactgcctac cactttgccc ccgaagcccc
     3301 cagcggctgg accatcggcg caggcgtgcg ctggcagagc gaaacccaca ccgaccctgc
     3361 cacgctccgc atccccaacc ccgccgccaa agcccgcgcc gccgacaaca gccgccaaaa
     3421 agcctacgcc gtcgccgaca tcatggcgcg ttaccgcttc aatccgcgcg ccgaactgtc
     3481 gctgaacgtg gacaatctgt tcaacaaaca ctaccgcacc cagcccgacc gccacagcta
     3541 cggcgcactg cggacagtga acgcggcgtt tacctatcgg tttaaat
//
LOCUS       DAXIHS010000123         3502 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXIHS010000123 DAXIHS010000000
VERSION     DAXIHS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.123"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            386..1195
                     /locus_tag="ACFOF2_001921"
     CDS             386..1195
                     /locus_tag="ACFOF2_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC7108171.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1212..>3502
                     /locus_tag="ACFOF2_001922"
     CDS             1212..>3502
                     /locus_tag="ACFOF2_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC7108172.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKM
                     GDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSA
                     AETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSIDWTQTANEGIALTPSQVAQLKKN
                     ALVSLSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAK
                     NIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEML
                     AYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNV
                     GFGHNKNVSLYGNDGNDTLIGGAG"
BASE COUNT         1118 a          664 c          769 g          951 t
ORIGIN      
        1 ttacagtcga tgaaccacca ttttgcttta tttcagcggg cttgagatgt gtataagaat
       61 attgttttga ataaatttaa agaaaatgat aatcgttatt gacgattttt aaaggaaagc
      121 gtagagtgcc aattctatga agcaatacgg taagtaacaa tgaaaatatc tactgcttgg
      181 gtatagagca tatttcacaa cccgtaacta ttcttgcgga aacagagaaa aaagtttctc
      241 ttctatcttg gataaatata tttaccctca gtttagttaa gtattggaat ttatacctaa
      301 gtagtaaaag ttagtaaatt atttttaact aaagagttag tatctaccat aatatattct
      361 ttaactaatt tctaagcttg aaattatgag accatatgct actaccattt atcaactttt
      421 tattttgttt attgggagtg tttttactat gacctcatgt gaacctgtga atgaaaagac
      481 agatcaaaaa gcagtaagtg cgcaacaggc taaagaacaa accagtttca acaatcccga
      541 gccaatgaca ggatttgaac atacggttac atttgatttt cagggcacca aaatggttat
      601 cccctatggc tatcttgcac ggtatacgca agacaatgcc acaaaatggc tttctgacac
      661 gcccgggcag gatgcttact ccattaattt gatagagatt agcgtctatt acaaaaaaac
      721 cgaccaaggc tgggttcttg agccatacaa ccagcaaaac aaagcacact ttatccaatt
      781 tctacgcgac ggtttggata gcgtggacga tattgttatc cgaaaagatg cgtgtagttt
      841 aagcacgact atgggagaaa gattgcttac ttacggggtt aaaaaaatgc catctgccta
      901 tcctgaatat gaagcttatg aagataaaag acatattcct gaaaatccat attttcatga
      961 attttactat attaaaaaag gagaaaatcc ggcgattatt actcatcgga actatcatag
     1021 gtatggagag aacgattaca gcactagcgt aggttcctgt attaacggtt tcacggtacg
     1081 gtattacccg tttattcggg aaaagcagca gctcacacag caggagttgg taggttatca
     1141 ccaacaagta gagcaattgg tacagagttt tgtaaacaat ccaagtaaaa aataatttaa
     1201 aggatcttat tatgaatgag ggtgaagttg ttttaacacc agaacaaatc caaaccttgc
     1261 gtggttatgc tttccgtggc gatacctatg gcggttggcg ttatttggct aatttgggtg
     1321 accgttatgc ggatgatgct gctgcaattg tcggtaagga tgcaaactta aatggtttga
     1381 atttatggat gaaaaaaggt gtggaaaacc tatgggatga tacggtcggt aaaaagaccc
     1441 gtttagagaa atttgatcgg gttgcattgc aacatttcag ccaatatgta gatctaatta
     1501 atgaaaataa tggtagatta cctaacacta gtgaaattga gagaagttac tataaagccg
     1561 ttaccgaaaa tggtgtttct tctagtgcag ctattgattt agttattaat cgctcacttc
     1621 cggatatggc agatggttat tgggcattag gtttggggat agaagccgaa cgtatccaca
     1681 atgcgcaagc agtaaataat ccgaacggta gcgaatggga taatagaaag cagttaatat
     1741 ctgctttaga taaaggattt gatggatctt ttaaagagaa gcattttact tttttacaat
     1801 ctgtgataat ggatgtaaca aagttaggtg ttgaatatac aatagatggt tggcaaaaaa
     1861 ttggaggttg gggtaatggg ataatcaatg atttatataa aagtgttgca aaaagagagt
     1921 ggactggaat atttgagatc gttaataata acatcaagca atttagagat ctgttcccaa
     1981 atccggaagg ctggatcgat gatggtcacc aatgtttcgc tccttgggtt aaagaaacta
     2041 aaaaacgcaa tggcaaatat catgtctacg accccctcgc cctagatttg gacggagacg
     2101 gtatagaaac cgttgccacc aaaggctttg caggcagctt atttgatcac accaacaacg
     2161 gtatccgcac cgccaccggt tgggtttctg ccgatgacgg tttactcgtc cgcgatttga
     2221 acggcaacgg catcatcgac aacggtgcgg aactcttcgg cgacaacacc aaactggcag
     2281 acggttcttt tgccaaacac ggctacgcgg ctttggccga attggattca aacggcgaca
     2341 acatcatcaa cgcggcagac gccgcattcc aaaccctgcg tgtatggcag gatctcaatc
     2401 aggacggcat ttcccaagct aatgaattgc gtacccttga agaattgggt attcaatctt
     2461 tggatctcgc ctataaagat gtaaataaaa atctcggtaa cggtaacact ttggctcagc
     2521 aaggcagcta taccaaaaca gacggtacaa ccgcaaaaat gggggattta cttttagcag
     2581 ccgacaatct gcacagccgc ttcaaagaca aagtggaact cactgccgaa caggcaaaag
     2641 ccgccaatct tgcgggcatt ggtcgtctgc gcgatttgcg cgaagctgcc gcattgtccg
     2701 gcgatttggc caatatgctg aaagcttatt ctgccgccga aactaaagaa gcacagttgg
     2761 cattgttaga taatttgatt cacaaatggg cggaaaccga ttcgaactgg ggcaaaaaat
     2821 cgccaatgcg actttcaatc gattggacgc aaacggctaa tgaaggtatt gcactgacac
     2881 catcccaagt agcacaacta aaaaagaacg ctttagtttc cctttctgat aaagctaaag
     2941 cagctattga cgccgcccgc gaccgcattg ccgtgcttga tgcctacacg gggcaggatt
     3001 ccagcacact ctattacatg agcgaagaag acgcgcttaa tatcgtcaaa gtaaccaacg
     3061 atacatacga ccatctcgcc aaaaacatct accaaaacct gttgttccaa acccgtttgc
     3121 agccatattt gaatcaaatc agtttcaaaa tggaaaatga tacgttcact ttggatttta
     3181 gtggtcttgt tcaagcattt aaccatgtca aagaaactaa tccgcaaaaa gcttttgtgg
     3241 atttggccga gatgcttgca tatggcgaac ttcgttcttg gtatgaaggc cgaagactaa
     3301 tggccgatta tgtggaggag gcaaaaaaag caggtaaatt tgaagattac cagaaagtgt
     3361 tgggtcagga gaccgttgca ttattagcta aaacatcggg tacgcaagca gatgatatcc
     3421 tgcaaaatgt aggctttggt cataataaaa atgtttcttt atatggtaat gacggcaacg
     3481 acactctgat cggcggtgcc gg
//
LOCUS       DAXIHS010000124         3440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXIHS010000124 DAXIHS010000000
VERSION     DAXIHS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.124"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(143..1066)
                     /gene="era"
                     /locus_tag="ACFOF2_001923"
     CDS             complement(143..1066)
                     /gene="era"
                     /locus_tag="ACFOF2_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC7108173.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1114..1833)
                     /gene="rnc"
                     /locus_tag="ACFOF2_001924"
     CDS             complement(1114..1833)
                     /gene="rnc"
                     /locus_tag="ACFOF2_001924"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC7108174.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            2000..2323
                     /locus_tag="ACFOF2_001925"
     CDS             2000..2323
                     /locus_tag="ACFOF2_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC7108175.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2371..2847
                     /gene="ribH"
                     /locus_tag="ACFOF2_001926"
     CDS             2371..2847
                     /gene="ribH"
                     /locus_tag="ACFOF2_001926"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC7108176.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2925..3350
                     /gene="nusB"
                     /locus_tag="ACFOF2_001927"
     CDS             2925..3350
                     /gene="nusB"
                     /locus_tag="ACFOF2_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC7108177.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
BASE COUNT          849 a         1117 c          743 g          731 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccact ataatatgca cagataatat caacccgttt
       61 ttaacaaaga tattcccgat tgaaacaaaa tgccgtctga aacctgttcg gacggcattt
      121 gcgtaaagtt cagcaagaaa aactacaaac ccagctcgcg caggaagcgg atgtcgtccg
      181 cccaaccgga tttgactttg acccagacct tcaaaaatac tttggtatca aacagttttt
      241 ccatatccaa ccgcgcttcg gtggaaatct tcttcaaacg ctccccgcct ttgccgatta
      301 aaatcgcctt ttggctttcc ttatcgacca aaacggcgat gtagatgcgg ttcaaaccgt
      361 cttcctcttc aaactgctcc acttcgacat tcatcgcata aggcaattcc tcgcccaaat
      421 agcggaagag tttttcacgc acgatttcca tcgctaaaaa acgcgccgat ttgtccgtaa
      481 ccatatcttc gggatacatc ggcacgcttt cgggcagata cggcttaatc agctccaaca
      541 ggttggcaat ccgcaatccg tgtttcgcgc tgaccgcctc cgccgccgca aattcaaatt
      601 cggcgcgcac ttgggcaaca aacgcctcca gcgcgtaacg gtctttcgcc ttgtccttgt
      661 cgattttgtt gaccactaaa atgaccggcg tgtgcttggg cagttgtttc aacacgacgc
      721 ggtcggcatc ggtaaagcgc atcgcctcca cgacgaaaac caccacatcc acgccgccga
      781 gcgcctcggt aacattttga ttcagcctgt cgttgagcgc gttgcggtgg tcggtttgaa
      841 agccgggcgt atcgacaaac acgaactgcg cggtatcgtc ggtataaatc cccgttacgc
      901 ggttgcgcgt cgtctgcgcc tttttgctgg tgatgctgat cttctgaccg atgagatggt
      961 tcatcagcgt tgatttgccc acgttcggac ggccgacaat cgccacgaag ccgcaacggt
     1021 atccgccggc ggcgcgttcc cctgcaagga aggtttcaat atccatattt gctttcgtat
     1081 ccatatgttc agacggcata ttcacgcgcc gcctcatttc tttttcttct tcagcggcag
     1141 cttctcttcc agccatttca aagcctcttt cgccgcctcc tgttccgccg ccttgcggct
     1201 cgtccctttg gcacggcaca cgaaacccag ttcgcccaaa tcgcaggaaa ccacaaacat
     1261 actgtcgttg gcatggccga tttgctcttc gatacggtat ttcggcaaag caaagcggcg
     1321 tgcctgcaac gcctcctgca aagcagtttt gccgtctttt gcctgatttt ggaaatcggc
     1381 acaccggacg cggtcggcaa acaaatggcg caccaccttt tccgccgtgt tgaaatcggc
     1441 atcgaagctg accgccgcaa acatcgcctc catcgcgtct gccagtatcg aaggccgtct
     1501 gaagccgccg ctcttcaact cgcccgcccc caagtacaga ccgtcgccga cattcatttc
     1561 cgccgccatt tccgccagca cgccctcatt gaccagactt gcccgcaacc gcgacaactc
     1621 gccctcggtc aacttcggaa acgtgtcaaa cagcatccgc gccaccgtat aattcaaaat
     1681 cgaatcaccg acaaactcga accgctcgtt gtgcttcgca tgatggctcc tgtgcgtcaa
     1741 agcctgccgc aaaagcgaaa tatcgcggaa cgcgtagccc agtctcttct gtatcgccgc
     1801 gtgtgcctgc tgtttcaaaa catcgtcttt cattaccgtc ttccgcaaaa aacagcccga
     1861 atgcgtttcc atactccaaa aatacagaaa caccttaagg ctgcaaacgg cgcacattgt
     1921 accaccaaac cgccgccgtc tgccgacaac gcaagaatcg tggtaaaatc cgcaagtttt
     1981 cccccaacgt attcccgcaa tgaacgcatt tgcacaagca ctttcctcgg cactcgaccg
     2041 ctgcatcgcc accaacaccg tcgccaaaca aaaccagccc gtcggcttcc tctaccgcga
     2101 agcccccgtt tttgaaaacg acagcggctg gcgtttcttc agcggcgacg agaccgacga
     2161 atacaccgac gacccggaca acttcagcat cgtcagcctc gccgatatca ccaaaaccaa
     2221 ccctgaaacc gccgcactgc tctcccaacc tgaaggcagc gcgtgggaat tggcagaaga
     2281 cggcacattc caaaccgtcg ccgactggca gccgcaagac taaaaccttc agacggcatt
     2341 ccgaaacctc cgaataccaa aggacaccca atgaacacca tcgcccccaa cctcgacggc
     2401 aaacacctcc gcatcggcat cgtacaggca cgcttcacca acgaaatcgg cagcgaaatg
     2461 ctcaaagtct gctgccgcac cctccaagaa ttgggcgtgg cagacgaaaa cattaccgtc
     2521 gccaccgtac ccggcgcgct tgaaatcccc atcgcgctga tgaactttgc ctcttctgaa
     2581 aaatttgacg cactgattgc catcggcgtc gttatccgtg gcgaaaccta ccatttcgag
     2641 ctggtttcca acgaatccgg agcaggggtc agccgcgtcg cactcgacta caacatcccg
     2701 attgccaatg ccgtcctgac cacggaaaac gacgcacagg caatcgaacg gattgaagaa
     2761 aaagcctcgg atgccgccaa agtcgccgta gaatgcgcca acctcgtcaa cctcctgctc
     2821 gaagaacagt ttgaagacga agaataacaa acagggcgta caccgatacg ccctttccgt
     2881 ttcagacggc ataccgtgcc gcccaaccgt ttcaaggaaa aatcatgaaa acagcccgcc
     2941 gccgttcccg cgagcttgcc gtacaagccg tttaccaatc ccttatcaac cgcaccgccg
     3001 cgcccgagat tgctaaaaac atccgcgaaa tgcccgactt tgccaaggca gacgaagaat
     3061 tgttcaacaa acttttcttc ggcacgcaaa ccaatgcggc agagtacatc cgacaaatcc
     3121 gccccctgct cgaccgcgac gaaaaagacc tcaaccccat cgaacgcgcc gtcctgctga
     3181 ccgcctgcca cgagctgtcc gccatgcccg aaacgcccta ccccgtcatc atcaacgaag
     3241 ccatcgaagt aaccaaaacc ttcggcggca cggacgggca caaattcgtc aacggcatcc
     3301 tcgacaaact cgccgcccaa atccgtcccg acgagcccaa acgccgttga tgcatcacaa
     3361 atttaaagaa agttctatgt ccttatttct gctttctttg tttcgtcatt cccacgcagg
     3421 tgggaatttg gttttttgaa
//
LOCUS       DAXIHS010000125         3417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXIHS010000125 DAXIHS010000000
VERSION     DAXIHS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.125"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..527)
                     /locus_tag="ACFOF2_001928"
     CDS             complement(<1..527)
                     /locus_tag="ACFOF2_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC7108178.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGN"
     gene            complement(585..775)
                     /locus_tag="ACFOF2_001929"
                     /pseudo
     CDS             complement(585..775)
                     /locus_tag="ACFOF2_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1530..1688)
                     /locus_tag="ACFOF2_001930"
     CDS             complement(1530..1688)
                     /locus_tag="ACFOF2_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108179.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLERTGAEVYINGRRVYHNEA
                     EMASASLR"
     gene            complement(1845..2264)
                     /locus_tag="ACFOF2_001931"
     CDS             complement(1845..2264)
                     /locus_tag="ACFOF2_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108180.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(2322..2687)
                     /locus_tag="ACFOF2_001932"
     CDS             complement(2322..2687)
                     /locus_tag="ACFOF2_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108181.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(2669..3214)
                     /locus_tag="ACFOF2_001933"
     CDS             complement(2669..3214)
                     /locus_tag="ACFOF2_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108182.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
BASE COUNT         1076 a          782 c          535 g         1024 t
ORIGIN      
        1 aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat cgaaacatga
       61 cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa taccgagaaa
      121 tgatcatcct tacggtattt cagatacacg atgacgggga ttttcaactg cgcgagctgc
      181 tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat gcgccgcata
      241 tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc ttcttccgtc
      301 agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa
      361 tccaaatcct gctttacaat attgaaatcc cgccgcgctt tccaactctg cactttgatt
      421 tttccgtaaa caacaggatt atcgttaaac atcggtgcag cattcagacg ataagataaa
      481 gaaacgacaa cacacgccaa cagaaaaaca tatttgaact tcatcatatt gtccacataa
      541 agggcagcct gaaaatcttt caggctgccc ttgtcaaatt attcctagct ttcggctttt
      601 ttggcaaacc aaacaatccg attacccgca taatactttc catttattga aatccgacaa
      661 gccgcgccca aaaatgccat gcactgtcga tttccgcagc aatctttgta ccgttttctt
      721 caaattccaa atattcaccc aataataaac ttgaaacaga acgcgtgggc agcaagtgcc
      781 caccctacgc ttattcaaat aatttgatta aataaagaat aaagaaaatg catcaggaac
      841 aaaattataa tctgccacct gactcacacc gcttttaaag taaggggcat caaaatcaaa
      901 accgcaaaaa aaataatttt tgcattgatt tttaatagat ttaaaattcg aatatagtgt
      961 ttctccagat ttaaaaagtt tatctttatc tatatatttg atgctttccc tatccaaaat
     1021 aaatattcaa acattaaaaa atcattacat gaccaagcca aatcataaat ttgattcaaa
     1081 tgggtatcat aaagataaaa ataataaggt ttgggaacag gtaaaatatt taatggaaaa
     1141 ggaggactaa ttttcttaat atctgatgac tgatatccat atctttctat attcttaaaa
     1201 atttcatctt tcttaagata acaagacatt ctgaagataa caagacattc tgacaatcac
     1261 aacctgttca tcggatatat tatctatttg catggcgcgt ataacacgcc atgcctgatt
     1321 aaaattagtc tcccttacct tagaatctat tatcttttcc aaagatgaaa atgcccctta
     1381 tcttgaactc tccagttaga acctggaagt ttcgtacctc ttaaatgtgt attaatattt
     1441 cttatagttt cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt cgtaccgctt
     1501 tcttcaaatt ccaggtattc ccccattagc taacgcaaag aagcagacgc catttcggct
     1561 tcgttatgat aaacccgcct tccgttgata tagacttccg cccctgtccg ctccaaattc
     1621 caaaaattcc gtactgaaat ttccatatcc cgatattgtg cagaccatgt tttttcgaag
     1681 gttttcataa aatttcctat acctgtccaa tcggcacata tcaattgcat tattatagtg
     1741 gattaacaaa aatcaggaca aggcaacgaa gccgcagaca gtacaaatag tacggaaccg
     1801 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg
     1861 ccgtactggt ttaaatttaa tccgctatac atctcaatac gataaatatt tcttaagtca
     1921 aaatgcaagc ctgaccgtac cttaactgtc aaaattttat tattttttat tgattttaaa
     1981 acaatttctg caaaattctc ttcgctttct ctctttttta gaagcacata agaaaaaata
     2041 aaacttcccc gattaaattc ataaatatgt ttcaaccatt tgcctcctct ttctgtaaga
     2101 caagattcag tttcattctt ccttattgta taaatatttc cttcacaaaa tctgaaataa
     2161 atccataaat ccatcttatc cataattaaa gaaaaagttt cacctcgaga ttttgtcaac
     2221 aattcgcaag gttgcgatgt tgcaatcaaa tagccgaaag acatttttta cctcatacat
     2281 ggtcgaaatc agtttctgtt agttcagaat ccattttttc gtcaacaact gaatccgcat
     2341 ttttgaatta acgttttcat cagctgccgt ttatctaaac cggcaggttc agtttcaaaa
     2401 taagccttat atgaagactg taagcatttc agaaaaagat catcagaaga catatctgcc
     2461 gaatcaaata caactgtttt gattttggta cttacccaaa accctttttg ctctttttct
     2521 actatacgga aattcagaat atttccaacc gaatcaaaag cacggtaaac atcatccatc
     2581 aaatcctgcg gctctatttt cttttccaat tccgacaatc cttgaaatat atccaaagac
     2641 acatcttcaa atagaaaaaa aggaggagtt agaagcggtt tttccatgat ctgtccgtag
     2701 attttgattc ccaagggcga tgacgaccaa ttccctgtcc aggcaaagtc ttgcccgtat
     2761 tatccgtaac tcgacgatga taatggggaa attttccaat aggatgacct gttctattac
     2821 cgaaaggggc tatccgcata ttattgccga ttttaatctc acgtccatat ttagcaaagg
     2881 aaacaacctt tcctgcggcg cctacaccac caggaattgc gcctaatccg ccagcaatag
     2941 caacatctct aacagaagct ggtctgcctg tcgttgcata actaaaacca tgctgtgtcc
     3001 acataccaat ggcagcacca cccaagatag ccaatggaag aaacgccctc tcagtctcct
     3061 tcatctcctt ttgagaaagt tccgccaact gcatcggcgc atctgcccgc gtatggaata
     3121 cttggttttc aaatatctga ttgtccatgc tgtttgccat tgcgggggca atcatagaca
     3181 gcatcattac ggctgcggta atttgttttt tcataataac tcctttggat tacaaggttg
     3241 gaaaatcaaa gccctgctta gaacgtatgt tgcacaccca atttcaatag gtaaatcaga
     3301 ttgcaaatcc agcaatttga atattgtcat tgttccgtgc aaaagggatt tttattgatg
     3361 agttgtgtac tgggtttcag cttggctttt tagataatct tttcacaaga acataca
//
LOCUS       DAXIHS010000126         3405 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXIHS010000126 DAXIHS010000000
VERSION     DAXIHS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.126"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(97..735)
                     /locus_tag="ACFOF2_001934"
     CDS             complement(97..735)
                     /locus_tag="ACFOF2_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC7108183.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            990..1832
                     /locus_tag="ACFOF2_001935"
     CDS             990..1832
                     /locus_tag="ACFOF2_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC7108184.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            1898..2560
                     /gene="exbB"
                     /locus_tag="ACFOF2_001936"
     CDS             1898..2560
                     /gene="exbB"
                     /locus_tag="ACFOF2_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC7108185.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2581..3015
                     /locus_tag="ACFOF2_001937"
     CDS             2581..3015
                     /locus_tag="ACFOF2_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC7108186.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          897 a          799 c          957 g          752 t
ORIGIN      
        1 taatgatgaa aaaaataaga ttacactaca ggaaaacgcg gcagggcaat atgtcctgcc
       61 gcgttttctg gagcgtttat gccgtctgaa agccagtcag acgaacatct gcacaaacgc
      121 aatcaccagc cgcacaatcg gtgcaataaa cgcacccaaa accccggtca gcatcagcag
      181 taggataatc cacgtcccat aaggttcgat tttgcggaac gcttgcgaat atttcgccga
      241 caggaaggtg tcgatgaaaa tgccgccgtc ccaaggcagg atggggatga tgttgagcgc
      301 gaacagaatc gcattgatca gaataccgta gtttgccatt tgagccaacg gcatctgata
      361 cgccccaccg acatacggag tcagcaccaa aaccacgccc cacagaacag ccatcgctag
      421 attcgacagc gggccggacg cggcaacgca acgccaggca aggcgcgggt tgcggaagtt
      481 gcgcgaatcg ataggaatcg gacgcgccca gccgaacagg aagggcgtga acatcaaagt
      541 aagcagcggt acgatgattg tgccgaccaa atcgatatgg ggcagggggt tcagtgtcag
      601 cctgccgtat tgttcggcag tgttgtctcc ccagtagcgc gccgtatagc cgcgcgccac
      661 ctccctgacg gtaatggcga gcagcacggg caggacggca agcagaaaca cgcccaaatc
      721 aaaattttga aacatggttt tcctttgaac agatgtggtg tgcagcccga aacggctgac
      781 agacagaagc ggcaatatat catctaacct gattttattg gaataagaaa tcagacggaa
      841 tcattttttc tgccggcccg ttatctgatg atttgctgta atacagcgtt taaacacggt
      901 ttgcctgtga acggaacaga cattcatatg accaaaaatt gtaaaaatag gttgcaaata
      961 ggaattgttg ctattattat gcccgaatta tggataaaga acgaatttta acccccgcag
     1021 tcgtgttttc cgtcgcactg ctgcatttgg caatggtggc attgctctgg caggcgcaca
     1081 agctgcccgt gatagagtca ggaaatgtta tcgaatttgt cgatttgggt gattttggcg
     1141 gaggggacgg tgcacccgaa ggcgcaggtg cgcctgctgc gcctgagccg caacctgagc
     1201 ctgagccgcc caaacctgtc gagccgccca agccggtttt gaagccggtg gttacgaaaa
     1261 aggcggatgc ggatattcag cagcctaagg aagagccgaa acctgaagaa aagccgaaac
     1321 ccgaagaaaa accgaaacca gagcctaagc cggaagcgaa gcctgtcccg aaaccggcgg
     1381 aaaaaccggt cgagaagccg tctgaaaaac ctgccgaaca tcccagcaat gcttctgcca
     1441 aagcagacag cgagcagggt aatggggaag ataagggaac cggtaccaaa ggagacggaa
     1501 cggggcgcgg agaaggcagc ggtaaaggta gcggcggtgt caaaggcgaa cacggggaag
     1561 gagccggcag cagcaaaggc aatcctttac gcgccaacgg cagcattccg cgcccggctt
     1621 atcccacgct ttctatggag aatgacgagc agggtacggt tgttttgagc gttttggttt
     1681 ctccgggcgg tcatgtcgag tccgttaaaa tcgtgaaaag cagtggtttt tctcggttgg
     1741 acaatgcggc acgcaaggcg gcgcaaaacg ggcattttca agccaatgcc tggacggagt
     1801 ttaaagtccc cgtcaagttt gaattgaatt aggcggttgc cgcagcggtt tgcggcagct
     1861 tggatgtttg attggttttt tcagtgttgg aatcattatg aatttgaaat tagtatttga
     1921 atcgggcgat cccgtcctga tcggtgtgtt tgtgttgatg ctgttgatga gtatcgtaac
     1981 gtggtgtttg gttgtcttgc gctgcatcaa gctgtatcgg gcgcgcaaag ggaatgccgc
     2041 cgtcaaacgg catatgcgcg atactttgtc gctgaacgac gcggtcgaaa aagtgcgcgc
     2101 cgtcgatgcg cctttgtcca aactggcgca agaggcattg cagtcttacc gcaactaccg
     2161 ccgaaacgaa gcgtccgaac tggcgcaggc tttgccgttg aacgagtatt tggtcattca
     2221 aatccgcaac agtatggcgc agattatgcg ccggtttgat tacgggatga ccgcgcttgc
     2281 ctccatcggc gcgaccgcgc cgtttatcgg gctgttcggc acggtttggg ggatttacca
     2341 cgccctgatc aatatcgggc aaagcgggca gatgagtatt gcggcggttg ccggcccgat
     2401 tggcgaggca ctggtggcga cggcggcggg tttgttcgtg gcgattccgg cggtgttggc
     2461 atacaacttc ctcaatcgcg gcacaaaaat actgacccag gatttggatg cgatggcgca
     2521 cgatttgcac gtccgcctgc ttaatcaaaa ggatagctga ttataaaagg atagctgatt
     2581 atggcatttg gttcgatgaa ttccggcgac gattctccga tgtccgacat caacgttacg
     2641 ccgttggtgg acgtgatgct ggtgttgctg attgtgttta tgattactat gccggtgctg
     2701 acgcattcca tccctttgga actgccgacc gcgtccgagc agacaaacaa gcaggacaaa
     2761 cagcctaaag accccctgcg cctgacgatt gatgcgaacg gcggctatta tgtcggcggg
     2821 gattctgcaa gcaaagtgga aatcggggaa gtggaaagcc gtctgaaagc cgccaaggag
     2881 cagaatgaaa acgtgattgt ggcgattgcg gcagacaagg cggtggaata cgattatgta
     2941 aacaaagctt tagaagccgc ccgtcaggca ggaatcacca aaatcggttt tgtaaccgaa
     3001 accaaggcgc aataaagttc gacgcttgaa cgaatgccgt ctgaaagtct ttcagacggc
     3061 atttcttatg tttaatcagc tatatatagt tgattaaaac aaaaatgaga cgacagagca
     3121 ccgtctccat ttctgcgcag gcgggaatac aaacatctga tttttctttt attttcaaac
     3181 attactgaaa tgggtaagtc tggagtcctg cctgcgcgag aataatgaaa atgtgcattt
     3241 ctaattttta tagtggatta acaaaaatca ggacaaggca acgaagccgt agacagtaca
     3301 aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagt
     3361 taaggcgagg caacgccgta ctggtttttg ttaatccgct atacc
//
LOCUS       DAXIHS010000127         3396 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXIHS010000127 DAXIHS010000000
VERSION     DAXIHS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.127"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(27..467)
                     /locus_tag="ACFOF2_001938"
     CDS             complement(27..467)
                     /locus_tag="ACFOF2_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC7108187.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(409..726)
                     /locus_tag="ACFOF2_001939"
     CDS             complement(409..726)
                     /locus_tag="ACFOF2_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC7108188.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(854..1132)
                     /locus_tag="ACFOF2_001940"
     CDS             complement(854..1132)
                     /locus_tag="ACFOF2_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108189.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(1139..1357)
                     /locus_tag="ACFOF2_001941"
     CDS             complement(1139..1357)
                     /locus_tag="ACFOF2_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC7108190.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(1431..1628)
                     /locus_tag="ACFOF2_001942"
     CDS             complement(1431..1628)
                     /locus_tag="ACFOF2_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108191.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(1633..1923)
                     /locus_tag="ACFOF2_001943"
     CDS             complement(1633..1923)
                     /locus_tag="ACFOF2_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108192.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQPVKKG"
     gene            complement(1968..3311)
                     /locus_tag="ACFOF2_001944"
     CDS             complement(1968..3311)
                     /locus_tag="ACFOF2_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC7108193.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
BASE COUNT          851 a          769 c          748 g         1028 t
ORIGIN      
        1 taccttttag gcggcaacag gcgcacttag cctgagacct ttgcaaattt gtcggtttcg
       61 gggtcgtatt ggtagcctcg tgcctgtatg tcttctttga aagtttcgta tacgtcgtgg
      121 gctaaaaggg ctgttccgac atagggaacc gcccttgtgc tgaatttcgc gcctaagcgg
      181 gcaagtttgc cgacccccgc caatacgccg gcgcgggaaa cgctggcggt tattttggcg
      241 ttgattcggg cttttgcgcc cgtagggatg tgctcaaaat ttgccgcttc tatgaatttg
      301 gcaaaactct tagaacttga atctaattta aaaccacttg attttaaatt attacttgat
      361 tgaaatttaa tttgtgatct ttctaatctt acaggttcag aataagaatt actcgaacat
      421 agaattaaaa tcagaactat cggtgtactc ttgataaacg aattcatgaa cagtttttcc
      481 gttctcttct gattttctta taaaaataac agattcatca gaaaaataaa tcttccaaat
      541 attatgtgcg actctcttat ttaagaaata agagaaactc tctaaaatat cgtactcttt
      601 tacgttatta acaaattctt caaattcttt ctgagcaatt aatgccatcg attgcccaaa
      661 atacttgcta gacggctgat atttataaag tgccaactgc gcctgagtga taaacggctt
      721 gttcatggtt ctgcctttca atgattgttt tgaaagcctg attttgacac cataacttca
      781 tgcgctcaat tcttaaacag aaccgccccg attaatacgg gtacggaaac gccgagataa
      841 aaataaaaat ccatcatttc aaaacctttt tcagcaggga aacaaagtaa acggacgcga
      901 ggatgccgaa tactatccaa cctgtttcaa gaccgctttg caggttgtct ttcggactgc
      961 attccgccaa taaaagcctt agcggctgac cgtccgacat cttccacagg ctgccgttat
     1021 attccggcct gacaatctgt ccgttttctt tgattcttgg tactaccaag ctgaaataaa
     1081 ggttttcagc ctggtgcttc tcaagacatt tatttccgac ttggtagtac atgccgtctt
     1141 acttcatcac tctcttaacg atggaaaaca caaaaagcgc ggcgaaaacg ccaacgacaa
     1201 tccaaccggc ttccataccg tccgcttttg cggcttccaa agcgtttttt gccgtatcgg
     1261 gcaacgttgc atttgcatgt gcggccaaag ccaggggagc agctgttaca acagccagtt
     1321 ttgcgccgta tttacggcag gtgttaataa atttcatgat attttccttt aaaaagtgtt
     1381 tggcggtaat ggatggagcg tttttcagac gaccgccgaa catccgaaaa tcagtctttc
     1441 aaaaatccga atacgacaaa ttcgtattgg ttgccgattt cttccaaacc tgcgttaatc
     1501 gcttcttcga agtcgtagaa ataatcggca ttggtgatta atttggtatg tccgatgtcg
     1561 cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac aacatagact
     1621 ttctgcattc aatcagcctt tcttaacggg ttgaaaaccg atgactttca gtttttgggt
     1681 tttgcccgta gtgacgattt ctacgttcag gtttgcttcg atcggaaatt gggcgtttcg
     1741 gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc tgcccaatgc
     1801 gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt cgaagctctt
     1861 gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa gttggatgtt
     1921 cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta agcccatgaa
     1981 atcggtagtc ttgcgaattt gtcgtaaatg aagttgttat agctttcttc attgttgacg
     2041 tgtttttgct gttcaagctg tttttcaaga ttctcgtaat attcgtacat atagtaaggg
     2101 tctttgtacg gtttgaatgc gggctgttca tgaatggctt gagctttcaa aaaggcgcag
     2161 tcgtaggctt cgggagccaa agacttgggc agcttgtgat gactcggctc aatcagttca
     2221 aacagtttgg ctttgtccaa ttcgggaaaa atgaatttca gaccgtttgc cgcacgtcca
     2281 aactgttttt ttacccattc aaggtagcgg tcggctgaaa tgaccttatc ttccttaacc
     2341 gcgtgtatgc gcgttgcctt ttggacgaat cgttcgcaaa tcggatatgc gccgccgaaa
     2401 tattcgcccg gattctgcaa aacttcaaaa gggataacga tgtcttttgc tttgaattca
     2461 atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt ttcatagacg
     2521 cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt ggtcattttg
     2581 gcttcatcgt cttcttccca atctgacccc aaacattcgc cttttggttt gacgtgatga
     2641 caggtaaaca tacctttatt tcggtcttca cgggcttggt tcgggctgta ttcgccgttg
     2701 aaaaagtctt ttgcgatgtc aacgcgtgtg atttttgggc ggattgcatt agtcaggaat
     2761 gcgaaaagtc gtgattccca gccttctttt gcgacgccgc aaccggtgcc ggtcagttcg
     2821 aaaaggatgg tattgttttg tccgccaaaa tggacgcgac cgtataggac gtcttccgaa
     2881 cccatcaacc aacagcgctc ataaaaacga ccgcccgagc ctttggactc tttgtagata
     2941 ccgaaaccga aaacttcttc ggcgagcatg gacgcggcgc gaatgaaatc ttcgtcttcc
     3001 aaaagactta cacgaacgcc gtatttatcg aaaaaggttt tttcatgaaa tgaaaagcta
     3061 atttgatcaa tgaaagccga atctgataca ccgcgccgaa gaggaacgcc taacaggttt
     3121 cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac cctgcctgcc
     3181 gtttcggttt ctgtaccccc cctgttagat aaggggggaa gatttgaagc ggttgccggc
     3241 ttcctgccgt ccgctaacgc gtccgtcatc acgccgacaa ccgcctttgt catcccttgc
     3301 ttatcttcca tggtgcgaat cctcaaaaac gggcaaaaaa aagccctgtt acttgtagaa
     3361 agtaaagggc gttaattttt gttaatcgtc ccttct
//
LOCUS       DAXIHS010000128         3276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXIHS010000128 DAXIHS010000000
VERSION     DAXIHS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.128"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..551
                     /locus_tag="ACFOF2_001945"
     CDS             <1..551
                     /locus_tag="ACFOF2_001945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7108194.1"
                     /translation="RFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFF
                     EHTDLPVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVES
                     VLCGTRVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMR
                     ALNYNPSLSHHIDRLTDILASV"
     gene            827..1390
                     /gene="gmhB"
                     /locus_tag="ACFOF2_001946"
     CDS             827..1390
                     /gene="gmhB"
                     /locus_tag="ACFOF2_001946"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC7108195.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            1419..2162
                     /locus_tag="ACFOF2_001947"
     CDS             1419..2162
                     /locus_tag="ACFOF2_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC7108196.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPYASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            2159..2851
                     /locus_tag="ACFOF2_001948"
     CDS             2159..2851
                     /locus_tag="ACFOF2_001948"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC7108197.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHSRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT          786 a         1082 c          777 g          631 t
ORIGIN      
        1 aacgcttctt tccacaacct agagaaactg ccgctctgcg cgccaaatac ggttttgccg
       61 accatgaaac cgttttccta tttccatcga ccggccacac gcgcaaaggc ctggaactgc
      121 ttgccgactt tttcgaacat accgacctgc ccgtcaagct tgccgttgtc ggctccccgc
      181 tcccccgccc tatgaaaaac gtcgtcggac tgggcttctg caccgatatg cccgaactct
      241 accgcgccgc cgactttacc attatggcct ccctgtatga acccttcggg ctggtcggtg
      301 tcgaatccgt cctatgcggc acacgcgtcg tcctctccga aaacatggca tgtacagagg
      361 tcatgaacga agaagccgga ttctttttct cacgccaaaa cccggaaacc ctagcgcaag
      421 ccgttgccca agccgtcagc cttaaaaaac agggcggaca ccgcctgtcc gacccgatgc
      481 gtgcactgaa ctacaacccg tccctgtcgc accacatcga ccgactgacc gacatactgg
      541 catccgtata acaaaatgcc gtctgaacga gttttcagac ggcattgctg cgttatagtg
      601 gattaacaaa aatcaggaca aggcaacgaa gccgcagaca gtacagatag tacggaaccg
      661 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaata
      721 ccgtactggt ttttgttaat ccactatatc acgcggcaca ggcgcatcac tgccgatatt
      781 tcagtataat gccacccccg acctgcccca acccaaagga aacgcgatga aactcatcat
      841 tctcgaccgc gacggcgtca tcaatcagga ccgcgatgac ttcgtcaaat ccgttgacga
      901 gtggatacct gtcgaaggca gcatggatgc ggtggcattt ctgacgcagg caggctgcac
      961 cgtcgccgtt gccaccaacc aatccggcat cgggcgcaaa tattttaccg ttcaaaacct
     1021 taccgaaatg cacgccaaaa tgcaccgcct cgtccgtcag gcaggcggcg aaatcaacgg
     1081 catctggttc tgcccgcaca ccgatgccga caactgcaac tgccgcaagc ccaaaccggg
     1141 tatgattgaa gacatcatcg gacgcttcaa cgcccaagcc tcggaaacct ggctggtcgg
     1201 cgacagcctg cgcgatttgc aggcaatcga tgccgtcggc ggcaaacccg cgctggttct
     1261 gaccggaaaa ggcaaaaaaa cgctctccca acacggacac gaattgcccg aacacacaca
     1321 ggttttcgat accctgctcg atttctcaca atacatcatg caggaaaaca ccgcaccgca
     1381 agccgactga acataccgca ttccgacaag gcaaaaccat gctcatcatc cgcaacctga
     1441 tttactggct gatactctgt tccaccctga ttttcctctt tccctttatg ctgctcgcct
     1501 cgcctttccg agacggggcg cacaagatgg cgcgggtctg ggtcaaaatc ctcaacctct
     1561 cgctcaaaca catcgtcggg ctcaaatacc gcatcatcgg cgcggaaaac atccccgacc
     1621 gccccgccgt catctgcgcc aaacaccaaa gcggctggga aacgctcgcc cttcaggaca
     1681 tttttccgcc gcaggtttac gttgccaaac gcgagttgtt caaaatcccc tttttcggct
     1741 ggggcttgaa actggtcaaa accataggca tagaccgcaa caaccgccgc gaagccaacg
     1801 agcagctcat aaaacagggg ttggtgcgca aaaacgaagg ctattggatt accattttcc
     1861 ccgaaggcac gcgccttgcg cccggaaaac gcggcaaata caaactcggc ggcgcgcgca
     1921 tggcgaaaat gtttgagatg gacatcgtcc ccgtcgccct caacagcggc gaattttggc
     1981 cgaaaaactc ctttctgaaa tatccggggg aaatcaccgt cgtcatctgt ccgaccatcc
     2041 cgtacgcaag cggcagcgaa gccgaattga tgggaaaatg cgaacacctc atcgaaacgc
     2101 agcagccgct catttccggc gcaggcccgt ttgccgccaa aatgccgtct gaaaccgcat
     2161 gaccgccttt gtccacaccc tttcagacgg catggaactg accgtcgaaa tcaagcgccg
     2221 tgccaagaaa aacctgatta tccgccccgc cggcacacat accgtccgca tcagcgtccc
     2281 accctgcttc tccgtctccg ctctaaaccg ctggctgtat gaaaacgaag ccgtcctgcg
     2341 gcaaacactg gcgaaaacac cgccgccgca aactgccgaa aaccggctgc ccgaatccat
     2401 cctcttccac agcagacagc ttgccctcac cgcccatcaa gacacgcaaa tcctgctgat
     2461 gccgtctgaa atccgtgttc ccgaaggcgc acccgaaaaa cagcttgcgc tgctgcggga
     2521 ctttttggaa cggcaggcgc acagttacct gattccccgc ctcgaacgcc acgcccgcac
     2581 cacacaactg ttccccgcct cctcctcgct gacctctgcc aaaaccttct ggggcgtgtg
     2641 ccgcaaaacc acaggcatac gcctcaactg gcggctggtc ggcgcaccgg aatacgttgc
     2701 cgactatgtc tgcatacacg aactctgcca cctcgcccat cccgaccaca gccccgcctt
     2761 ttgggaactg acccgccgct tcgcccccta cacgcccaaa gcgaaacagt ggctcaaaat
     2821 ccacggcagg gaacttttcg ccttaggctg acgcggaccg gaccgacccg ccgctttcag
     2881 acggcatccg tgccggaaca ggcacgcgcc cgcccgattc aaaccgcgat gacgctttgc
     2941 cgccggttcg gggcaggatg gcggcacaca cgccgtctgc cgcgtttcat ttcacaccac
     3001 tcttccgaaa cccgaaaccc gcccggtccg acgtgcggta tgaaacgctt aagctgacgc
     3061 gaagtctttt actgatttgc ccgcgaaaat gccgtctgaa aggttttcgg acggcatttt
     3121 tttgcgtttc ccaggatggc ggcggattcg taaaaggcgg tcagggtgga ttgtaggatg
     3181 ggttgagacc tgccgaatcc gccgcatctg ccaaatctac cgccgtcatt cctacgaaag
     3241 tgggaatcta gaacgcgggg ttaagaaaac ctgcat
//
LOCUS       DAXIHS010000129         3189 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXIHS010000129 DAXIHS010000000
VERSION     DAXIHS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.129"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            68..238
                     /locus_tag="ACFOF2_001949"
                     /pseudo
     CDS             68..238
                     /locus_tag="ACFOF2_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
     gene            308..691
                     /locus_tag="ACFOF2_001950"
     CDS             308..691
                     /locus_tag="ACFOF2_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC7108198.1"
                     /translation="MYCLRLRRLVLIFVNPLYIIATTVPAQQTFLDAAELMQWSIETE
                     WLGLNVIAHCNFGKQHAQVKFEACFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPA
                     MKQPCICGSGKKFKACCGKYLKPVA"
     gene            complement(910..1704)
                     /locus_tag="ACFOF2_001951"
     CDS             complement(910..1704)
                     /locus_tag="ACFOF2_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC7108199.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1913..2638
                     /locus_tag="ACFOF2_001952"
     CDS             1913..2638
                     /locus_tag="ACFOF2_001952"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC7108200.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          854 a          934 c          722 g          679 t
ORIGIN      
        1 tttgggtaga attaggagat atttggggcg aaaacagccg aaaacctgtg ttgggatttc
       61 ggttgtcgtg agggaaagga attttgcaaa ggtctcaagc tatgccggct gctgccgccc
      121 acttcatctg cgccaaatcc tgccgcccac cgccgaagcc ctcatgcgtt cgcggtacag
      181 cgcgtatgtg ctgcacctta tcgactatag tggattaaca aaaaccagta cggcgttgcc
      241 tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc
      301 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
      361 catcatcgct actaccgttc cggcgcaaca aacattcctc gatgccgccg aactgatgca
      421 atggagtata gaaaccgaat ggctgggctt gaatgtcatc gcacactgca acttcggcaa
      481 acagcacgcc caagtcaaat ttgaagcctg cttccaagac ggacaacacc gatccgcaca
      541 tcacgaactg tccggcttcg tcaacatcgg cggacaatgg tattttatcg atcccaccgt
      601 tccgcatcct gcgatgaaac aaccctgcat ttgcggatcg ggcaaaaaat tcaaagcctg
      661 ctgcggcaaa tatctgaaac ctgtcgcata aaaatgccgt ctgaacgttc agacggcatt
      721 ttcaacgtgc aaaaaaaaac cattcatacc aagggtaagt atgaatggtc aatacattgc
      781 gggaaaacgt cttacttgct gcactgccga aaagggagaa acggcagcgg taatcagcgg
      841 aaaggattgt acccgaatta atgttaagaa acgttaatcg tgaaaatata ttaacaaacc
      901 tgttgaaacc tattggtttt cccgtatcca ctcgacccag cgttcaaaca gcttcggttc
      961 gagcgcggcg acgaccgagc gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc
     1021 caaagtcgtc agcctgccgc ccgcctcctc gaaaatcaac gcgccggcgg cataatccca
     1081 cagcttctgc ccgccgtgga cataaacatc ataacgcccg cacgccagat aacaccagtc
     1141 caacgtactg ctgcccatac tccgtatcgt tccaaaaggc gcgagcgtac tcatgcggct
     1201 ggacagtttg cccgaacgca gatatttgat ttccacgccc gcgattgcct cattcaactt
     1261 cttatccacg agacgcaaag gcaggcgtgt cccgtttaaa aacgcaccct gcccgcgttc
     1321 agcataaaaa cattcgccgc tgaccggatt gtagattacg cccaattcgg cgcgcccgtt
     1381 gcggacaaac gccaccgaca cggcaaaatg cggcagcccg ttgacaaaat tgtttgtccc
     1441 gtctatcgga tcgacaatcc acagcccctt ttcccccgaa tattgttccc acagagccga
     1501 ctgttcctgt cgcgacattt cctcgcccaa catcgggcaa tcgatcagaa gcggcaacgc
     1561 ggcggcaaaa gccgtctgcg cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt
     1621 gcggtgggac ggcgtattca aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac
     1681 gactttctgc aaacggtgta acacttccta ctgtcctcat attttgaact tgcggcacgc
     1741 gaacgtatat agtggattaa atttaaatca ggacaaggcg acgaagccgc agacagtaca
     1801 aatagtacgg caaggcgagg caacgctgta ctggtttaaa tttaatccac tataatgtcc
     1861 gtttccatca cgccgctgcg acagattata accgtccgaa ccgccaaaaa ctatgccccg
     1921 attccacctg cccgaaaacc tttccgtcgg acaaaccgtc gccctgcccg acaacatcgt
     1981 ccgccacctc aacgtcctgc gcgtccgccc caacgaaaac atcaccctct tcgacggcaa
     2041 aggcaaggca cacgccgcac ggctgaccgt tttggaaaaa cgccgcgccg aagccgaaat
     2101 cctgcacgaa gacacaaccg acaacgagtc cccgctcaac atcacactga tacaatccat
     2161 ctcctccggc gatcgcatgg atttcaccct gcaaaaaagc gtcgaactcg gcgtaaccgc
     2221 catacagccc gtcatcagcg aacgctgcat cgtccgcctc gatggggaac gcgccgccaa
     2281 acgcctcgca cgctggcagg aaatcgtcat ctccgcgtgc gaacaaagcg gcaggaacac
     2341 cgttcccccc gtgctgccca tcatcggcta tcgtgaagca ctcgacaaaa tgccgtctga
     2401 aagcaccaag ctgattatga gcatcaaccg cgcccgcaaa ctcggcgaca tccgccaccc
     2461 gtccggcgca atcgtcttta tggtcgggcc cgaaggcggc tggacagaac aggaagaaca
     2521 acaggcattt gaagctggct ttcaggcggt tacactcggc aaacggattt tacgcacaga
     2581 aaccgcccca ctcgccgccc tcgccgccat gcagacgctt tggggcgatt tcgcataaac
     2641 agaaatgccg tctgaaaccc gttcagacgg cattttgcag ccgattaaga tagtaggttc
     2701 aaataagatt tcccgtgtcg tcattcccgc gaaagcggga atctagaaac gaaaaactac
     2761 agggatttat ccgaaacaac aaaccctctc cgccatcatt cccgcaaaag cgggaatcta
     2821 gaaacgaaaa actacaggga tttatccgaa acaacaaacc ctctccgccg tcattcccac
     2881 gaaagcggga atctagaaat ttaacgttgc ggtgatttat cggaaatgac tgaaactcaa
     2941 cggactggat tcccgcctga gcgggaatga cgaattttag gttgctgttt tttggttttc
     3001 tgtttttgcg ggaatgacga gattttaggt ttctgttttt ggttttctgt tctcgcggaa
     3061 ataacgaaat tttaagtttt aggaatttgt cggaaaaaca gaaatcccgc cgccgtcatt
     3121 cccgcaaaag cgggaatcta gaaacgaaaa actacaggga tttatccgaa acaacaaacc
     3181 ctctccgcc
//
LOCUS       DAXIHS010000130         3149 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXIHS010000130 DAXIHS010000000
VERSION     DAXIHS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.130"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(99..311)
                     /locus_tag="ACFOF2_001953"
     CDS             complement(99..311)
                     /locus_tag="ACFOF2_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC7108201.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(323..604)
                     /locus_tag="ACFOF2_001954"
     CDS             complement(323..604)
                     /locus_tag="ACFOF2_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108202.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(651..854)
                     /locus_tag="ACFOF2_001955"
     CDS             complement(651..854)
                     /locus_tag="ACFOF2_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108203.1"
                     /translation="MNIIEQEMKHSSLRKVDAEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(934..1506)
                     /locus_tag="ACFOF2_001956"
     CDS             complement(934..1506)
                     /locus_tag="ACFOF2_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC7108204.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1481..1825)
                     /locus_tag="ACFOF2_001957"
                     /pseudo
     CDS             complement(1481..1825)
                     /locus_tag="ACFOF2_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1818..2693)
                     /locus_tag="ACFOF2_001958"
     CDS             complement(1818..2693)
                     /locus_tag="ACFOF2_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC7108205.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2738..3028)
                     /locus_tag="ACFOF2_001959"
     CDS             complement(2738..3028)
                     /locus_tag="ACFOF2_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC7108206.1"
                     /translation="MAESKRVQRLLRVFITLDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3040..>3149)
                     /locus_tag="ACFOF2_001960"
     CDS             complement(3040..>3149)
                     /locus_tag="ACFOF2_001960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC7108207.1"
                     /translation="RENGFKPRDVYLVVGGQRKGNYGKGHEIAKKLGLK"
BASE COUNT          523 a          847 c          886 g          893 t
ORIGIN      
        1 tgagtttatt ttggggcggc ggcaggtcgg gggcaagcgg tgtggggctt ggttgtggtt
       61 tttaggtttt tgggggtaaa aaatgccgtc tgaacttttc agacggcgtt tgttttttct
      121 gtccaatcga ggaactgccg ccatttttcc agcggcatat cggcccgccg cgtttcggta
      181 tcgggttcgg cttcccagcg gcctacctgt atgtagtgct tcaccccgac gatttgcgcc
      241 aactcggcct gtgtcagttt gcagcggttg cgcagggtgc gaaggttgta aggcgtgtag
      301 ccgagttcca tgtcgttgcg gtttattttg tttcgcatat ttttttgact gcccggcggc
      361 aggtttcggt aaggacggcg gcaaatcggg cttcgtctgc cggttttccg tcgaaaaaaa
      421 taaaatcgta aagggtgatg ccgttttcgg aatggcggta atgcccgccc gggcattcgc
      481 tgtctgtctg cctgtggatt ttggcaatca ggcggggata gcggcaatgg gtaatgaaac
      541 ggcttgcgcc gtcttgcccg ataatccatt cggggtagcg gttgaatagt gcggctctgt
      601 tcattttgtt cgggttgttc gggggataaa gcccctcgcg gggcttgtgg tcaggcaaat
      661 ttgaatatca gtgccaacac ggcggcgatg gcggtaacca gcccggtggc cgccatcatg
      721 ggataccatc gggattcttg ggctattttt acggattcgg cgttgatttt gtgcgcgtct
      781 gcaatgattt tggcgatttc ggcatctact tttctcagac tggagtgttt catttcttgt
      841 tcgattatgt tcatcgtact tcctttcgtt tttggcggtt gccgccgctt gtcggatggt
      901 aggatgtctg ccatgtgtat atattgatac cttttaggtt ttattgcaag tgttttgggc
      961 ggcggcttcg tatgcttggc ggtggcggcg gctgtaccat tcggcgagtt cgcgccgctc
     1021 ttggaggcgg tagctgtcgg cggtcaggta gtggtgcagg cttgagaagc cggcgcgttg
     1081 gagggcggcg cggctgatgt ggccgagggc gaggtctgtt gtgagggtgt cttttccggc
     1141 ggtcaggctg atgttggtga agaggtggcg gaatccgtgc attgtgtgtt tggatttgcc
     1201 gggggtgctg ccgtcatagc ccagtcgtcg gatggcgttg tgggcgaatt tgatgctgat
     1261 gtggtcggga tggggggcgg gtttgcgccg tgggcggatg ccggggaaaa ggtggatgtt
     1321 gtcgccggtc tgtgtgtgca gctctcggag tatttctacc gcccagtccg acagtgggac
     1381 ggtaaagggg tgtttggtct tcatgtcggc ggcggggatg tgccataacc gggcggtgag
     1441 gtcgatgtct tgccagcggg cggaaagcag tgcggaggga cgggatgact tccccggttt
     1501 ccccatcaat ccgcgccgcc tcgatttttc cgttggcgcg cagcatttcc accgcctgcc
     1561 gcaccgacat caaccgcttg ccgttgcgcc tcatcgcctc caccaaaacc gccgaaatca
     1621 atttggcttc ttccggttta agctccgtct tgcccgcatc ggtgcgccgt ttgcgcgtcg
     1681 gcttgacgct gaccgcctcc agcttgcggt atagcgtggc aaggctgatg cccaattcct
     1741 gcgcctgctg cttaagatat gcagagcgtg cgccgcgttc cattgcttcc gcctgattct
     1801 cgactgcctt aagacgctca atcattgccg gattcatcgc cttctcccgt ttcaccgccc
     1861 aaccattccg gcacattgtc tgtcggtgct tcagtcggca gggcatagct ttcgcgcagt
     1921 tgctcgcagt ccaaaataat ttgattgagc gtgccgacca tctttgcctg atggctgatc
     1981 ccgtgtgcct cactgtgcgc attaagttgg tcgaacaaat ctttcagacg gctcacttga
     2041 ctgcggatac cgacctcaag gcttgttaac tgcatcgcca actcgctgcc tacgtcttcc
     2101 gccttcggct ctctgacaac ggtttgcttc ttagccagct tctcggccag ctcatcaatt
     2161 ttggctgttt tggttttcat cacttcgtct ttggcggcga ggttttcgcg gctttcgcgc
     2221 agggcgacgc gcagctcgcg caccgtcatt cggtccacat cgtcaaaggt catgccgttg
     2281 acttcttccc cttcggccaa acccaccagc gtaacgtctt cttcgaccaa gagttccagc
     2341 agcttcgact tgcccaaatc catcagcttc ggcgcggctt tctgcatttg cggggtcgca
     2401 aagcggcgag tggctgacat cagacgtgat gtttctgcga tacccagccc aaattggctt
     2461 ttcacaattt ccataaaccg tccgtgttcc gtatgctctt ttaaaataat cagcgcacgt
     2521 cccagttcga acatgccttc catcgtctgc cgtactgctt gacgaccgcg ttcaacccaa
     2581 cgctcttcgc tataagtctc gccgttaccc cactgctcca tcaccagcac actgcgcatc
     2641 gctgcctgat tgcttacttt gtcggttgcg ataatttcaa tttctgtatt cattttttat
     2701 ctccaaagtt tccgacgtcg gaaactttca aaatccgtta atccacatcg acccgcttgc
     2761 cgatttcggc aatcttgctt tgcagccgtt catgctgctg cctgaaccgc tctgcgattt
     2821 gcagggtttt gatgccgtag gcgtagttgc cgttttcaag tttgatgacc aatcccgagg
     2881 caaccaaatc atcaatatcc ctgctgactt gcgatggcgt cagccccagt ccgaccgata
     2941 aatccttatt gctcagaccg ataatcggat gctcgtcaag cgtgataaag accctcaata
     3001 gccgttgtac ccttttactt tctgccatcc gcatcctcct tatttcagtc ccagcttctt
     3061 ggcaatttca tgccccttgc cgtaattgcc tttgcgctgt ccgccgacca ccagatacac
     3121 atcgcgcggc ttaaagccgt tctctcttg
//
LOCUS       DAXIHS010000131         3114 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXIHS010000131 DAXIHS010000000
VERSION     DAXIHS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.131"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(17..1312)
                     /gene="tyrS"
                     /locus_tag="ACFOF2_001961"
     CDS             complement(17..1312)
                     /gene="tyrS"
                     /locus_tag="ACFOF2_001961"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC7108208.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1375..3048)
                     /locus_tag="ACFOF2_001962"
     CDS             complement(1375..3048)
                     /locus_tag="ACFOF2_001962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC7108209.1"
                     /translation="MKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSAL
                     TALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQ
                     FGFLAALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAVGRRCAAV
                     GALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQSKITV
                     AAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKW
                     QGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWG
                     DTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAA
                     CRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQA
                     VYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAKYPNVYII
                     DAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(3045..>3114)
                     /locus_tag="ACFOF2_001963"
     CDS             complement(3045..>3114)
                     /locus_tag="ACFOF2_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC7108210.1"
                     /translation="ISGFLMTTILHREMSGGGGGFP"
BASE COUNT          730 a          751 c          922 g          711 t
ORIGIN      
        1 ttctgcggca atcggattat ttccaaacca aaagcgcgtg gttgcgtttg ccgcgccgaa
       61 ggatggtgta tttgccgaaa cgtttgtgtt cgtcggtcag caggcaggca tcgtcggggc
      121 gttcggcggc gtggttgggg ttgttggctt cggcaggttt gccgttgagc aaaaccgctt
      181 tgctgttcac aaagccgcgc gcttctttat tggaggatgc caaaccggtt tttaccaaga
      241 cttcgacgac attgatgccg tctgaaactt caaatgcagg cagaccgtcg agggcgagct
      301 gctcgaagtc gctttcggtc aggctgcttt ggtcttcggc aaacaggctt tcggaaatgc
      361 gttgcgcggc ggcaagggct tcttcgccgt gaatcaggcg ggtcatttct tcggcgagga
      421 tgcgttgcgc ttcgggcttg ctgccgcttg ccttgtcttt ggcttcgatg gcatcgattt
      481 cttcgatgga caggaaggta aagtatttca ggaatttata cacatcggca tcggcgactt
      541 tcagccagaa ttggtagaac tgatagggcg aggttttttt cgcgttcagc cataccgcgc
      601 cgccttcggt tttgccgaat ttggtaccgt ctgatttggt taccaaaggc agggtcagac
      661 cgaatacttg tttttggtgc aggcggcggg tcaggtcgat accggcggtg atattgcccc
      721 attggtcgga gccgccgatt tccaaaaccg cgccgtggcg tttgttcaac tcggcgaagt
      781 cgtaaccttg cagcagggaa taggcgaact cggtgaagga aatgcctgcg ccgtcgcggt
      841 cgatgcgctg tttgacggat tctttgttca gcatggcgtt gacggagaaa tgcttgccga
      901 tgtcgcgcag gaagtcaagg cagttcatgc tgccgaacca gtcggcattg ttcgccataa
      961 tggcggcatt tccgccttca aagctcaaga aaggggttaa ttggttgcgg atactttcca
     1021 cccagccggc aacagtttcg gcggaattca agctgcgttc ggcggctttg aagctggggt
     1081 cgccgatcat accggtcgcg ccgcccacca aagcaatcgg cgtatgcccc gcctgttgga
     1141 agcggcgcaa tgccaatacg ggcagcaggt gtccgatgtg caggctgtcg gcggtcgggt
     1201 cgaagccgca ataaagggca attttttgtt cgttcaacaa agcgtctaag gcttcgatgt
     1261 cggtggtttg cgcgataagg ccgcgcgatt gcaggtcttg gatgacgctc atcggtctct
     1321 ttcaaaaaaa attagcgttt ttgcaaaccg ccgattgtaa caaatttaag cgaatcaatg
     1381 gttatggcgc gtatcgagaa accgttgttt ttcggaaaaa cgctttgcca attccgtgcc
     1441 gccgtaaggg ttgatgtggt ctttgtccga gtaaaccggc aatccgccga tttgaaaatc
     1501 tgcggggata taggcggcgg catcaataat atagacgttg gggtatttgg ctgccaattc
     1561 cctgatgcgt gcattggctt tcagggtgct ttcgtcgtcc gggcgcaggg cttggcggta
     1621 acccggtatg cgtgaagaca agatataggc gcgctggacg ttgtaagacg aggcaaggtt
     1681 gtccgccatc aggtaaacgg cttgtttttc ggacgagagt ttatgcagca tacggtcgaa
     1741 tttttggaaa aaaccggcat cataggcaag ggagcggctg ttttcgggca tttggctgcc
     1801 ccagcgcatc gccaaaacca cttttgaata ccggggcagg tgttcttcgg catagcgata
     1861 aacggcgcgg caggctgccc agttttggaa cacacgggac gcgtagcctt ccacataggc
     1921 gcaagcgtcg gcggaaacca tagtggcgga ccatttttct tttttgccca cggcatcgaa
     1981 gaatgttttg taatggtcgg cgtgggagtc gcccaaaacc agcagttccg gctgtttttc
     2041 cgtatccccc cataggcatt gtttgccggt attgttgtgg caggaggtgt tggaacgcgt
     2101 cagccccaag cggtcgtatt gcgccataaa cggcagtctc atcgcaaaaa acgagcccgc
     2161 ccccaaaatg agcataggca aggcataaat ccataaaacg gattgtgcga acgaaccttg
     2221 ccatttttta aacggttttt cgatgcagtg gtaagaaaac agggaaagca gcaatatcag
     2281 gacgaccgcc gccgccggcg aataaggcgg caggttgtcc gggccgatat agcgcataaa
     2341 ggccaatatc ggccaatgcc acagataaag cgaataggaa atcaaaccgg cggcaacagt
     2401 gattttcgat tggaaaaatt ttttaagcgg gtgttcgtaa tgattgaaat aaatcagcgc
     2461 ggcaacagcc agacagggaa tcaaagcggc ggggcccggg aaataggcgg tttgttccga
     2521 ataggaaaac aggcaggttg acaatatgca cacggcaaac aatgcgccga cggcggcaca
     2581 gcgtctgccg acggcaggtt gccggcagcg catccacacg gcggtcagcg atcctatcag
     2641 taattcgcag gcgcgcaggt ggggcaggta atatttatcg agcgcggaag gtataaagga
     2701 ggcggcaagg cttaaggcac acagtgcggc aaggaagccg aactgtacgc gcaggctttt
     2761 gcgggcgaca agcagcagca gtatcggaaa gacaaagtaa aattgttctt cgaccgacaa
     2821 agaccagatg tgcagcaggg gcttttcttc ctgcgcggga tcgaaataat ccttccccct
     2881 tgcaaaatac aggttagagg cgaaacccaa ggcggtcagc gcggatttcc acaaaagaaa
     2941 gaaatcatct ttggtgaata aaaagaagcc gcctgccagc gttgccgcca atacggcgaa
     3001 aaatgcgggc agaatccgct tgatgcggcg gatataaaat gccttcaggg aaaacctccc
     3061 cccccccccg acatttcgcg gtgaagaatc gtcgtcatca aaaagcctga aatc
//
LOCUS       DAXIHS010000132         3045 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXIHS010000132 DAXIHS010000000
VERSION     DAXIHS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.132"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            132..353
                     /locus_tag="ACFOF2_001964"
                     /pseudo
     CDS             132..353
                     /locus_tag="ACFOF2_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(335..592)
                     /locus_tag="ACFOF2_001965"
     CDS             complement(335..592)
                     /locus_tag="ACFOF2_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108211.1"
                     /translation="MTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            1259..2314
                     /gene="aroG"
                     /locus_tag="ACFOF2_001966"
     CDS             1259..2314
                     /gene="aroG"
                     /locus_tag="ACFOF2_001966"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC7108212.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            2382..2870
                     /locus_tag="ACFOF2_001967"
     CDS             2382..2870
                     /locus_tag="ACFOF2_001967"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC7108213.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
BASE COUNT          779 a          657 c          815 g          794 t
ORIGIN      
        1 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
       61 tgaagcacca agtgaatcgg ttccgtacta tttgtactat ctgcggcttc gtcgccttgt
      121 cctgattttt gttaatccac tatattttag ataatgcgtg atttcaccgt atgggtgtct
      181 tacgggaaat ggcggaaaaa ttgggacata aggtattgcc tcttgcacct tattcacctg
      241 agctcaaccc gattgagaaa gtgtgggcga atattaagcg gtatctgcga accgttttgt
      301 ctgattacgc ccgatttgac gatgcactac tgtcctattt tgattttaat tgactataga
      361 acgttgcggc tacgcggaag ccgtactcgt tggatttgga gcggcccatt ttggttttgt
      421 cgccgtccaa aacaatctca cggggtttgc agattgtttt gtgacggtag tatggatcaa
      481 actcgagacc gacgctgtcg gtcaactgtt tgcctacatt cagaccgata ccgacactcc
      541 aacctttggc gcttttgctg acatcgcggg aagcacacat ctgggtcgtc atcactttgg
      601 ttttgccgcg caaatctgca tatgcatccg cccaaggggt tagggatcat ccgtccccca
      661 aatcttggcg gatttcgcca tggactttca aagcaaggtt ttcatgcttg gtaacggtgt
      721 ttttccttat cgccgatgat ggctttgcct ttgccgttag actcgggaat atcggctacc
      781 gtaacggcgg acacggctgc aagtgagagt gcaagcaggg ttttttcatg tttttcttcc
      841 tataatgagg ataaataaat ggaaaaagtg tgggaaatac ccgcattccc attaaatctt
      901 ttttcaagca atgagttctt tttgttttca acattttcct tgtgtatgaa attttgccgg
      961 atgtgcaggc ggaatcggca gcggggtgtt ctgtaccgga ttgtcgtgga aatgggaaaa
     1021 cggatgttcc gtgcggtttt gtccgaatga atggcgggta ttgtttttat caatctgttt
     1081 ctttttcttt ggaataaaat tcctaaaata ttaaaaatat gaaatttaaa atctatgaaa
     1141 aaagatatat cagttatttt gaaataaaat agctttgtag taatatgttg cacttgtttg
     1201 tgcaaggtaa acgatgtaac ctaagccgcg tataaaaaac catcaggaaa gatgcaagat
     1261 gacacaccat taccccacag acgatattaa gattaaagaa gttaaagagt tgttgccgcc
     1321 gattgcccat ctttacgagc tgccgatttc caaagaggct tcgggcttgg ttcaccgcac
     1381 ccgtcaggaa atttccgatt tggttcacgg cagggacaag cggctgttgg ttattatcgg
     1441 gccgtgttcg attcacgatc cgaaagcggc gttggaatat gcggagcgtt tgttgaaact
     1501 ccgcaagcag tatgaaaacg agcttttgat tgtgatgcgc gtttatttcg agaagccgag
     1561 gacgacggtg ggttggaaag gtttgattaa cgacccgcat ttggacggta cgttcgatat
     1621 caatttcggt ttgcgtcagg cgcgcagcct gttgttgtcg ctgaacaata tgggtatgcc
     1681 tgcctctacc gagtttttgg atatgattac gccgcaatat tatgcggact tgatttcttg
     1741 gggggcaatc ggtgcgcgga cgaccgaaag ccaagttcac cgcgaattgg caagcgggct
     1801 gtcctgcccc gtcggcttta aaaacggtac ggacggcaat ttgaagattg ccatcgacgc
     1861 aatcggtgcg gcgagccatt cgcatcattt cttgtctgta accaaggccg gacattccgc
     1921 cattgtccat accggcggca atcccgactg tcatgtcatt ttgcgcggcg gaaaagagcc
     1981 gaattatgat gcgggacacg tcagcgaggc ggcggaacaa ctgcgtgcgg caggggtaac
     2041 cgataagctg atgatagatt gcagccacgc caacagccgc aaggattaca cccggcagat
     2101 ggaagtggca caagatattg ctgcccaatt ggaacaggac gggagcaata ttatgggcgt
     2161 gatggtggaa agccatttgg tcgaaggcag acaggataag ccggaagtgt acggcaaaag
     2221 cattaccgat gcgtgtatcg gttggggcgc gactgaagaa ctgttggcat tgttggcagg
     2281 tgcaaacaaa aaacgtatgg cgcgcgccgg ttgagatttt tgacacggaa tgtcataaaa
     2341 tgccgtctga agcgttcaga cggcattttt gtggaggaaa tatgctcaaa ataaccctaa
     2401 ttgcggcgtg tgcggaaaac ctgtgcatcg gggcgggcaa tgctatgcct tggcacatcc
     2461 ccgaagattt cgcatttttc aaagcctata ccttgggcaa acccgtcatt atggggcgga
     2521 aaacgtggga atccctgccc gtcaaacccc tgcccggacg gaggaacatc gtcatcagcc
     2581 ggcaggcgga ttattgcgcg gcaggcgcgg aaacggcggc aagtttggag gcggcattgg
     2641 cattgtgcgc aggcgcggaa gaagccgtca ttatgggcgg cgcgcagata tacggacaag
     2701 cgatgccatt ggcgaccgat ttgcggataa ccgaagtgga tttgtctgtg gaaggagatg
     2761 cgtttttccc cgaaatagac cggacgcatt ggaaagaggc agggcggacg gaacgccgcg
     2821 tcagcagcaa aggcacgcgc tatgcttttg tgcattattt gagatattga aatataaact
     2881 ctctataaaa tcccccgcaa atgatgggct gaaatagaaa atattgttat tcccccgaag
     2941 atgggaatcc gggattttaa agttagggta atttatccga aataacaaca atcttccatc
     3001 gtcattcccg caaaagcggg aatccggaaa cgaaaagcta aagca
//
LOCUS       DAXIHS010000133         3028 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXIHS010000133 DAXIHS010000000
VERSION     DAXIHS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.133"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            82..243
                     /locus_tag="ACFOF2_001968"
     CDS             82..243
                     /locus_tag="ACFOF2_001968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108214.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            293..577
                     /locus_tag="ACFOF2_001969"
     CDS             293..577
                     /locus_tag="ACFOF2_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108215.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWAIGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(702..1418)
                     /gene="trmB"
                     /locus_tag="ACFOF2_001970"
     CDS             complement(702..1418)
                     /gene="trmB"
                     /locus_tag="ACFOF2_001970"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC7108216.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1540..2943)
                     /locus_tag="ACFOF2_001971"
     CDS             complement(1540..2943)
                     /locus_tag="ACFOF2_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC7108217.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT          641 a          625 c          910 g          852 t
ORIGIN      
        1 atacgattaa agtaatgtcg gattcaagaa tccgacctac ggaaaactaa agattttata
       61 cgggctacgg cttgctggct tatgcgttgg cttaatttga aaaacagcac atcggttcat
      121 aatacacaag gtattagaac aaaagaagca gttgaacagg gattgcgtgg taataataga
      181 cctgaaaaag gaataggtaa tagatggaat tatgatggat ttataaagga attagcgaag
      241 taacaggtat tccaaatatt tctgataaat ataaagaaca aggagtaaaa aaatgaaagt
      301 aatcactatt acattgttta ctagctgcat actatctgga tgctatgggc ttgaatatgg
      361 aatgagtggc ggcattcctc atgatgactg ggcaataggt agccatgtta gtcaagccaa
      421 agcagatgct gcgtggaaag aatgttctga caaatatatg agaaatggtg gttcaggatt
      481 agtagaacaa tgtatgaatg ataaaggatt aatacgttta tctgaaatac gtcgtaggca
      541 aaagagagca gaaacacaaa gagcagtaat taagtaacaa taatgaaata ctactgttcc
      601 cgaacaaagc ggcatttacg caggtgatga cggctatatt ttgaaaaatg ccgtctgtaa
      661 gcaaacgtgc ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat
      721 accaagtccc aaacgccgtg tccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg
      781 cggtagtccg gggtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctgctg
      841 aggacttcaa gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg
      901 ccggttttga gtttgggcag cagtttggcg atgaaggggg cttgtatcag acggcgtttg
      961 tggtggcgtt ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg
     1021 tcttgcagca tattttcgac aacttctacg gcgtcgtgcc gcatcacgcg gatgttttcc
     1081 aaatggtttt cttctatgag tttgagcagg ttgcctacgc cgggaccgtg tacgtcgatg
     1141 gcgagaaagt cggtttcggg cagcctgcgg gcgatttctg cggttgctgt ccccataccg
     1201 aagcctattt cgaggatttt ggggcggttg cttccgaaat tggcatcaag atcggccggt
     1261 gttgcttggt aatcgatgcc gaagctgtcc cataaggtat cgatggcgcg ttgctgcgcg
     1321 gcggtcatat ggctttggcg gaggacgaaa ctgcggatgc tgcggggatg tccggcttga
     1381 cggtcgtttg gtgtttggtt ttcggcggtg tctgtcataa tgcttccgtg cgcttgtgca
     1441 atcaggatgc cgtctgaatg tgaaaggcct gttcggacgg cgggaaggga aaggggcgat
     1501 tatacctgtt tcgtttcggc agggtaggtg taacgctgtt cagacggcat tttgccattg
     1561 ttgcagcagt tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag
     1621 gtagcggcag gctttttctt tgtccggccc gacaccgtgt ccgctgtaat ggatgcaggc
     1681 gaggttgtag aacgccatac tgtctttttt tgcggcggct ttttcaaacc atttgcgtgc
     1741 ttcattgtag tcggcggtca taccctgtcc gaagtaatac atgctgccca gattggtttg
     1801 ggctttgctg tgtccgagtt ctgcggcttc tgtgtagagt ttgagcgcgg ctgcgtattc
     1861 gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagct gttctgccct
     1921 ttgccgccta atggcggaat ctttaatgcc gccgtattgg tcaggatggt ttaacgcgct
     1981 gtcggatatg agtttctgat aggcggtaag gctgccggct tcggctgcct gccggtaata
     2041 atgccgggct ttttccgtat cgggggctat gcccaaaccg tagcggcaga tgtcgcccag
     2101 tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcgtgat gcagggcaag
     2161 tttgtagttt ctttccggat gcttgccttg caggtagatg tcggcaagtg cggcatgggc
     2221 atcggggtca ttttcctgtg cggcctgttg aaaccggtaa acggctttat cgggagccgt
     2281 aggtaggatg agctcggcaa gcggggtgta ggcgggaatg tatccttgtt gtgcggcggc
     2341 gcgcaaatga ttgatagctt gggtcgtgtc ggttggtgta ccttgtccga aacggtattg
     2401 aaggccgagc tgccattgtg ccgccgccaa gccttgtgct gcagcctcgc ggtagagcag
     2461 gtgtgccgtt tcaaaatccg gcggcgtgcc gtaccggcag tattgggcaa gcaggtattg
     2521 cccgtggggg ttgttgtttt ttgcgcttgt ttttgcccat ttcagggctt cttcaaaatg
     2581 tcgggacgca tacagcaggt acatcaggcg gacttgtgct tcaggcagcc cttcctctgc
     2641 taatggtctg taaagcgata tcagggtttc cgggggtgcg ccttccaatt cttggatata
     2701 tgccagattg taacgcgcgt agggatgtcc ctgttcggcg gcgatgcgga gcatttccat
     2761 accttgttcg cggtcttggt aggggctgtc cgcgtccatc aggtgttttg ccaactcgaa
     2821 tgcggcggcg ggatgacctg ccgaggccgc ttctgtcagc agtcggcgga cttcggtaaa
     2881 gttatattgt ttttgagaca ggcgttctaa ggcctgccga tagggtaagt cgggattgtt
     2941 catatcagat cgttttccgt tttgtcgaat catcgttatt gtgttatgaa gttaggttta
     3001 tagtggatta acaaaaatca ggacaagg
//
LOCUS       DAXIHS010000134         3027 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXIHS010000134 DAXIHS010000000
VERSION     DAXIHS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.134"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(234..710)
                     /gene="greA"
                     /locus_tag="ACFOF2_001972"
     CDS             complement(234..710)
                     /gene="greA"
                     /locus_tag="ACFOF2_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC7108218.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1102..2403
                     /gene="aroA"
                     /locus_tag="ACFOF2_001973"
     CDS             1102..2403
                     /gene="aroA"
                     /locus_tag="ACFOF2_001973"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC7108219.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIATTPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2446..2979)
                     /locus_tag="ACFOF2_001974"
     CDS             complement(2446..2979)
                     /locus_tag="ACFOF2_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC7108220.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          640 a          826 c          813 g          748 t
ORIGIN      
        1 ttcccgcttt tgcgggaatg acggcgacag ggttgctgtt atagtggatt aacaaaaacc
       61 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
      121 agtgaatcgg tttcgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt
      181 attaatccac tataatttcc tgcgtgtgtc gggtgtatcg aaatcaagcc gaatcaaata
      241 tatcggactt cgataatgtc gtattcgcgc acgccgcccg gggcttggac ttccgccgta
      301 tcgccctctt ccttgccgat taaggcgcgg gcaatcggtg agccgacata gattttgccc
      361 tgtttgatat ccgcttcgtc ttcgccgaca atttggtagg taacgtgttc ttccgtttcc
      421 aaatcttcca gcgtaaccgt cgtaccgaac acgattttgc cttcggcgtg gatttcggtc
      481 ggattgatga tgtgggcaac ggaaagtttg tgttccagct cggaaatgcg gccttcgata
      541 aagccttggc gttctttggc ggcttcatat tcggcgtttt cggacaaatc gccgtgcgat
      601 ctggcttcag caatcgcttc gatcacttcg gggcgcgcca cgcttttaag ctgctgcaat
      661 tcctgtttca gcaattccgc accgcgtacg gtcaggggga ttttttgcat cggtcttttt
      721 tctccatatt ccggcacacc ggtttgcggc agcaaacata ccgcataccg tcttgttttg
      781 tgcgtccgga tattaaaata aaaacacaag ccgcccggaa aatcggcggc ttgtctgtcg
      841 ttgaacagcg gctattctac caaattctat gaaattggca atcgtgccgt gccgtccgca
      901 aacacgctat gtccgcaaca aaagccgaaa atatgccggc aaaaaaattt tagaaacaaa
      961 aaatttaaaa ataatcaatt ttcggcacaa aaaaccatat tcacggactt taaaaccgaa
     1021 aatgccaaac ctgagatttt tcagacggca tttgcaccag tataatgcag gctgttttta
     1081 tctttatatt tacgttttgc catgaccgaa tccctccgcc tccctgccgc ctcgctcaaa
     1141 ccttccactg tcgccctgcc cggctccaaa agcatcagca accgcaccct gctgcttgcc
     1201 gccttgtccg acaatgcttg cgaaatccat tccctgctca aatccgacga taccgaccgt
     1261 atgctcgaag cactcgataa actcggcgtt gaaatcgaat atcttgccga agaccgtctg
     1321 aaagtgcacg gcacaggcgg acgcttcccc aaccgcactg ccgatttgtt tttgggcaac
     1381 gcgggcacgg cgttccgccc gctgaccgcc gcgcttgccg ttttgggcgg cgattatcgc
     1441 ctgcacggcg ttgcgcgtat gcacgaacgc cccatcggcg atttggccga tgcactgcgg
     1501 attgccggcg ccgatgtcga atatctcggc aaggaacact atccgccgct tcatatcggc
     1561 gaacgccaag acaacggcga gcgcgtgatt ccgattaaag gcaatgtatc cagccagttt
     1621 ctgaccgccc ttttaatggc gttgccgctg accgggcagg cgtttgaaat ccgtatggtc
     1681 ggcgagttaa tttccaagcc ctatatcgac attactttaa aactgatggc gcaattcggc
     1741 gtacaggtta tcaatgaagg ctaccgcgtc ttcaaaattc ccgccgatgc gcactatcac
     1801 gcgcccgaac acttgcacgt cgaaggcgat gcctccagcg cgtcctactt cctcgcagcc
     1861 ggtttgattg ccaccacgcc cgtccgcgtt accggtatcg gcgcaaacag catacagggc
     1921 gatgtcgcct ttgcctgcga gctggaaaaa atcggtgcgg acgtggtttg gggcgaaaac
     1981 ttcgtcgaag tctcgcgccc gaaagaacgt gccgtccaag cctttgattt ggatgcaaac
     2041 catatccccg atgccgccat gaccctcgcc gtcgtcgcgc ttgctacagg gcaaacctgc
     2101 acgcttcgca acatcggttc gtggcgcgtc aaagagaccg accgcattgc cgcaatggcg
     2161 aacgagttgc gcaaactcgg tgcaaaagtc gccgaagaag ccgaagcaat tcacatcacc
     2221 ccgcccgaaa cgccgacacc cgacgccgtc atcgacactt acgacgacca ccgcatggca
     2281 atgtgtttct cgctggtttc gctgttggac gtacccgtcg tcatcaacga tccgaaatgc
     2341 acccacaaaa ccttcccgac ttattttgaa gtgttctcat cgctgaccga aacagcggaa
     2401 taaggcggca ttttgccgtg attccggcgc agcgcggcgg gcggctcatt ctgtaaaaaa
     2461 agtatgtgcg ccgaggtagt ttttggcgta aaacggtgtg gagagttttt cggttttgat
     2521 ggttttgccg ctgctggggg catggatgaa ttcgccgttg ccgatataga gtccgacgtg
     2581 tgagtagcgg tgtgtgccgc cggtgttgaa gaatacgagg tcgccggcct taaggcggct
     2641 gtcggggatt ttgcggcttg ccgccgccat gtcgcgggcg gtgcgcggca gcttgacgtt
     2701 gagggcgttt ttgtaaacga attgaatcat gccgctgcaa tcgaagccgg ttgcggtgct
     2761 gctgccgccc catttgtagg gcgtgccgat gagtccgagg ctgtggagca tgagttcctg
     2821 cgagccttgt gtgcggtcga tgtggctgat gcggacggct tggatttgcc ggactgtctg
     2881 tttgggtttc ggttggcggt gtttgccgga ggtcgtgccg catgaggcga ggagcagtgc
     2941 gctgagacag aggaaaaggg ttttgtcggg gggaaacatg gtttttcctt tgcgggttcg
     3001 gatatccgtc tgaaggcgtt tcagacg
//
LOCUS       DAXIHS010000135         3018 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXIHS010000135 DAXIHS010000000
VERSION     DAXIHS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.135"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(293..1927)
                     /gene="groL"
                     /locus_tag="ACFOF2_001975"
     CDS             complement(293..1927)
                     /gene="groL"
                     /locus_tag="ACFOF2_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC7108221.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(2020..2310)
                     /locus_tag="ACFOF2_001976"
     CDS             complement(2020..2310)
                     /locus_tag="ACFOF2_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC7108222.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2540..>3018
                     /locus_tag="ACFOF2_001977"
     CDS             2540..>3018
                     /locus_tag="ACFOF2_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC7108223.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSATDILGIHPNSAVLFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGWV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADR"
BASE COUNT          642 a          774 c          781 g          821 t
ORIGIN      
        1 acgtaaaatc tgaagaaacc gttttatccg atacgtttcc gcaccgacag acctagattc
       61 ccgctttcgc gggaatgacg gcggaaaggt tgctgttttt ccgataaatt cctgccgctc
      121 ttcgtttttg ggatggcggg aaataaaaca aaagcgcgcg tatcaaaaaa caaaaatgca
      181 aagaacggcg ttggcaattt taaggtttgc gccttagccg cacttattcg caaaaaaaac
      241 cgcacggcgt tgaccgtgcg gcttgttgtc tgaaagcttc agacggcatt tcttacatca
      301 tgccgcccat accgcccatg ccgcccatgt caggcatagc aggtttttct tcagggattt
      361 cagcgatcat gcagtctgtg gtcagcatca gaccggcgat agatgcggcg tgttgcagcg
      421 cggaacgggt tactttggca ggatcgagta cgcccatttc aatcatatcg ccgtattcgc
      481 cgctgccagc gttgtaaccg tagttgcctt tgccttccaa cactttgttc acgactacgc
      541 tgggttcgcc gcctgcgttg gcaacgattt ggcgcagcgg agactcaatg gcgcgcaaga
      601 cgatttgtac gcctgcgtct tgatcggcat tgccggtgtg caggttttcc agagcggcac
      661 gggcgcgcaa cagggctacg ccgccgcctg caaccacgcc ttcttcaacg gctgcgcggg
      721 tagcatgcag cgcgtcttct acgcggtctt ttttctcttt catttcgact tcggtagcgg
      781 caccgacttt gattactgcc acgccgcctg ccagtttggc cacgcgctct tgcagttttt
      841 ctttgtcgta atcgctggtt gcggtttcga tttgttggcg gatttcggca acacgcgctt
      901 cgatttgggc tgcgtcgccg aagccgtcga tgatggtggt gttttcttta ccgatttcga
      961 tgcgtttggc ttgacccaag tcgtccaaag tcgctttttc cagagacagg ccgacttctt
     1021 cggaaatcac cgtgccgccg gtcaggatgg cgatgtcttg cagcatcgct ttgcggcggt
     1081 cgccaaagcc cggagcttta acggcaacgg ttttcaggat gccgcgaatg ttgtttacca
     1141 ccaaagtcgc caaggcttcg ccttctacgt cttcagcgat aatcaagagc ggacggctgg
     1201 cttttgccac ttgttccaaa acaggcaaca agtcgcggat gttgctgatt tttttgtcga
     1261 acagcaatac aaacggattg tccaagccgg cgatttgttt ttccgcatcg ttgatgaagt
     1321 aaggagacag gtagccgcgg tcgaattgca taccttcaac cacgtccagc tcgttttcca
     1381 aagatttgcc gtcttcaacg gtaatcacgc cttctttgcc gactttttcc atcgcttcgg
     1441 caataatcgc gccgacttgt tcgtcggagt tggcggaaat cgagccgact tgggcgattt
     1501 ctttagaagt gtcgcaaggt ttggcgatgt ttttcagctc ttcaaccaaa gcggcgacgg
     1561 ctttgtcgat accgcgtttc aggtcggtcg ggttcatacc ggcggtaacg tatttcatac
     1621 cttcggcaac gatggattgc gccagtacgg tggcggtagt cgtaccgtcg cccgccacgt
     1681 cgttggtttt ggacgcgact tcttttacca tttgcgcgcc catattttca aacttgtctt
     1741 tcagttcgat ttctttagcg acggttacgc cgtctttggt aatgtgcggg ccgccgaaag
     1801 cgcggtcaac caccacgttg cggcctttgg gacccagggt tacgcgcacg gcgtttgcca
     1861 gaatgttcac gccgtttacc attttttgac ggacttcatt gccgaattgt acgtcttttg
     1921 ctgccatttc aattctccaa aaatcattaa aactgtctga taaaaccgtt tatgccgtct
     1981 gaagacggtt tgccgtttca gacggcatcg tgtccgtatt tatttttcaa cgatgccgaa
     2041 aatatcttct tcgcgcatta ccaacagctc ttcgccgtcg gcttttacgg tttggccgct
     2101 gtatttgccg aagatgattt tgtcgccgac tttgacatcc agcggacggc gcgcgccgtc
     2161 tttaccgatt ttgcccgcgc ccacggcgat gacttcgccc atgtcgggtt tttcggcggc
     2221 cgcacccggc aaaacgatgc ccgatgcggt tttttcttca gcttccaagc gtttgacgac
     2281 aacgcggtcg tgtaaaggac ggatggtcat atttatgctc cgataaaatg atttgaaaac
     2341 aatcatctgc ccgaacggtt cggacagatt gaagtggaaa ccgggctgcc gcctgccggc
     2401 ccgcccgtat aagtcggcaa aattagggtg tgtgcgggta aattcaagtg aggcggaaaa
     2461 aaatttattt ccgccgtttt ttatagtgga ctaaatttaa ggggctgtac tagattagca
     2521 gatatgttac cctcgaaata tgaagataac gcactgcaaa ttaaagaaaa aagtacagaa
     2581 agaactgctc catttttttg tactggaagt taccgcccgt tctgccaccg atattttggg
     2641 tatccatccc aattcggcag tactgttcta ccgtaaaatc cgcacggtta tcaaccatca
     2701 tttggccttg gctgccgatg aggtttttga gggccctgtc gagccggacg aaagcgattt
     2761 cggcggacgg cgtaaaggca gacgtggtcg cggtgcggca ggaaaagtgg ttgttttcgg
     2821 cattctgaaa cgcaacggat gggtctatac cgttgtggtg gataatgcca agtctgaaac
     2881 gttactccct gtcatcaaaa agaaaatcat gccggacagt attgtttata ccgatagtct
     2941 gagcagctgc gacaagttgg acgtgagcgg ttttatccat taccgcatca accattccaa
     3001 ggagtttgca gaccgtca
//
LOCUS       DAXIHS010000136         3018 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXIHS010000136 DAXIHS010000000
VERSION     DAXIHS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.136"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            110..2878
                     /gene="tdfH"
                     /locus_tag="ACFOF2_001978"
     CDS             110..2878
                     /gene="tdfH"
                     /locus_tag="ACFOF2_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC7108224.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRTGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          860 a          757 c          730 g          671 t
ORIGIN      
        1 attgaaatct tcagacggta tatcgaattt acacttttta atgtttatgc cgcctaaaaa
       61 aatgctaata tatttcttaa ttgtctgact gtttattgtt gaggaaaata tgagatcttc
      121 tttccggttg aagccgattt gtttttatct tatgggtgtt atgctatatc atcatagtta
      181 tgccgaagat gcagggcgca cgggcagcga ggcgcagata caggttttgg aagatgtgca
      241 cgtcaaggcg aagcgcgtac cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc
      301 gacccgtcag gatatattca aatccagcga aaacctcgac aacatcgtac gcagcatacc
      361 cggtgcgttt acacagcaag ataaaagctc gggcattgtg tctttgaata ttcgcggcga
      421 cagcgggttc gggcgggtca atacgatggt ggacggtatc acgcagacct tttattcgac
      481 ttctaccgat gcgggcaggg caggcggttc atctcaattc ggtgcatctg tcgacagcaa
      541 ttttattgcc ggactggatg tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag
      601 ccttgccggt tcggcgaatc tgcggacttt aggcgtggat gacgtcgttc agggcaataa
      661 tacctacggc ctgctgctaa aaggtctgac cggcaccaat tcaaccaaag gtaatgcgat
      721 ggcggcgata ggtgcgcgca aatggctgga aagcggagca tctgtcggtg tgctttacgg
      781 gcacagcagg cgcagcgtgg cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg
      841 aaattttggc gcggaatatc tggaacggcg caagcagcaa tattttgtac aaacaggcgg
      901 gttgaaattc aattccaaca gcggaaaatg ggaacgcgat ttgcaaaggc aattctggaa
      961 aaccagttac tataaacaat acaaagaccc ccagaaactg aaaaaataca tcgaagacca
     1021 agataaagac tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggcaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat cagttcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 gcttttaaag gattttgaaa cctacaacaa cgcgaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgcg aaaccgagtt gcaaaccact ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtcctga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca gggaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggactgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggga tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacatc gcaatttcaa agacaaagtg cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggctga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagctc gttcgacccg aaagacaagg acgaagaagt
     2761 aacgtgtaat gatgataaaa cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgatgacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtagaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccattt
//
LOCUS       DAXIHS010000137         3008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXIHS010000137 DAXIHS010000000
VERSION     DAXIHS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.137"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(125..976)
                     /locus_tag="ACFOF2_001979"
     CDS             complement(125..976)
                     /locus_tag="ACFOF2_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC7108225.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1324..2838
                     /gene="katA"
                     /locus_tag="ACFOF2_001980"
     CDS             1324..2838
                     /gene="katA"
                     /locus_tag="ACFOF2_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC7108226.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
BASE COUNT          724 a          834 c          791 g          659 t
ORIGIN      
        1 gtttctagat tccctctttc gcgggaatga cgggatgcag gtttccgcgc ggacggattc
       61 ggcattcccg ctttcgcaga aatgccggct tttggtttgt tgtctttata agattattcg
      121 gcaattattc tgcggttttg cgtttgtgtg ctgccggaat ctcaaaggat tctctgtatt
      181 tggcaatggt gcggcgagaa acctccatgc cgcggaaagc cagcaggttg gcgagcgcct
      241 cgtcagaata cggcgtgggt gtggacgagg caaaaccggc ggcgggaaag ggattgagcg
      301 aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc ggtttggggc aggcgttttt
      361 ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct gtcgagggcg tttcggacga
      421 tatgcagggc ggagggtttg gcagcacatt cgcccgatct ttctatctgc agtatcagcg
      481 attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa ttttttcaat gcggtcaggg
      541 cttgtttcag cattgcttca tccaacatcc actccaaggg cgtatggtcg aggatgtctt
      601 cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc gatgaggatg tggacacagg
      661 cggattcttg gtcggaaagc gggtgttcgc atacttgcgc gtgcaggtat tgcttgaaat
      721 cctgctcgcc ggcgatgttg gacagcatat cttcgccttc gtctccgccg atttgacggg
      781 caggcgcagt gtaatggctg aactcggcat cggaaaattc atccgtgtct ttgcgttcga
      841 gcagggggtt gtccgacagc cagttttcga cctcgcgttc aagttcgata cccgacatct
      901 gcaatacgcg caaagattgt tgcagccgct ggttgagctg ctgggtctgt ttgagcttta
      961 ttccgagtaa ggtcatgata atgtggggaa aattgttatt ttcagcctgt cggcgcaaaa
     1021 aatgccgcaa agcgtcattg cattataaat ggttttaatg agcgggttcg gattccgttc
     1081 gataacaaaa aacaaacgaa aatcaagaac cgattgctta taataatatt aaatcgattt
     1141 catagtttta atagcgaaaa tcttggcgta tagtcgcatc catagttttt acaaaaggga
     1201 aataaaatgt cgattcaaga aatttattgc aatcaagaaa ccggttacga atacgctttc
     1261 cgccaaatcg tactgtaagc cgtttacccc catttgacca acctgagaaa aggaacaaga
     1321 gcgatgacta cctccaaatg ccctgtaacc catctgacca tgaacaacgg cgcgcctgtt
     1381 gccgacaatc aaaacagcct gaccgccggt cctcgcggcc ctctgctggc gcaggatttg
     1441 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1501 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc acgacatcac caaatacacc
     1561 cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1621 gtggcaggcg aacgcggcgc agccgatgca gaacgcgaca tccgcggttt tgcgttgaag
     1681 ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1741 cgcgacccgc gtaagttccc cgacctgaac aaagccgtca aacgcgaccc gcgcaccaat
     1801 atgcgctctg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc actgcaccaa
     1861 gttaccatcg tgatgagcga ccgcggcatc cccgccggct accgccatat gcacggcttc
     1921 ggttcgcata cctacagctt ctggaacgaa gcaggcgagc gtttttgggt gaaattccat
     1981 ttccgcaccc aacaaggcat taaaaacctg accaacgaag aagccgccaa aatcatcgcc
     2041 gacgaccgcg aaagccatca gcgcgactta tacgaagcca tcgaacgcgg cgagtttccg
     2101 aaatggacga tgtacatcca agtcatgcct gaagcagacg cggaaaaagt accttatcat
     2161 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2221 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2281 ccgagcaacc tcgttcccgg tatcggcgcg agcccagata aaatgttgca agcccgtttg
     2341 ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga acttccgcca aatccctgtc
     2401 aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggacg cgccgacggc
     2461 aactacggca gcctgccgca ctacgaaccc aatagcttcg gccaatggca gcaacaaccc
     2521 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cgcactggga ctaccgccaa
     2581 gacgatgacg actatttcag ccaaccgcgc gccctgttca acctgatgaa cgacgcgcag
     2641 aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg acgcgcccga cttcatcaaa
     2701 taccgccata tccgcaactg ctaccgttgc gatccggcat acggcgaagg cgtggcaaaa
     2761 gccctcggac tgactgtcga agacgcacaa gccgcccgcg cgaccgaccc cgcactcggc
     2821 cagcccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     2881 gccgtccgct accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgc
     2941 ttcagacggc atttccccat cccgcccccg ccgtttcagt gggcgttttt tattaaacgc
     3001 aaaatatc
//
LOCUS       DAXIHS010000138         2925 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXIHS010000138 DAXIHS010000000
VERSION     DAXIHS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.138"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(145..235)
                     /locus_tag="ACFOF2_001981"
     tRNA            complement(145..235)
                     /locus_tag="ACFOF2_001981"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Ser,seq:gga)
     gene            complement(274..360)
                     /locus_tag="ACFOF2_001982"
     tRNA            complement(274..360)
                     /locus_tag="ACFOF2_001982"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Leu,seq:cag)
     gene            complement(396..482)
                     /locus_tag="ACFOF2_001983"
     tRNA            complement(396..482)
                     /locus_tag="ACFOF2_001983"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Leu,seq:cag)
     gene            571..>2925
                     /gene="rnr"
                     /locus_tag="ACFOF2_001984"
     CDS             571..>2925
                     /gene="rnr"
                     /locus_tag="ACFOF2_001984"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC7108227.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVK"
BASE COUNT          764 a          735 c          781 g          645 t
ORIGIN      
        1 ataccgcaaa acataccata aaaaaaacgg taacggatag ttgtaagcgg ttgatgacga
       61 ttatagacaa taacgggttt tcccaatgaa attattgttt gaataataaa aaatcccaaa
      121 ccgtaaaagt ttgggatttg tatttggcag agaggaaggg attcgaaccc tcgatacgct
      181 attcacgtat acacgctttc caggcgtgcg acttaaacca ctcatccacc tctctaatgg
      241 cggaaattat cccaatcggg ataatttatt atttggtgcc cgggagaaga ctcgaacttc
      301 cacacccatg aggataccag cacctgaagc tggcgcgtct accaattccg ccacccgggc
      361 aatctaaata ttgaaataaa gcaaagcatt tgatttggtg cccgggagaa gactcgaact
      421 tccacacccg tgaggatact agcacctgaa gctagcgcgt ctaccaattc cgccacccgg
      481 gctttgcttt atttgctgtt ttgcggtaca gtgtcctgcc gcaaagaagt cgctattata
      541 tagttcataa agagaatgtc aacagtccaa atgaataaaa atattaaatc tttaaattta
      601 cgggaaaaag acccgttttt aagtcgtgaa aaacagcgtt atgaacatcc tttgcccagt
      661 cgggaatgga taatcgaatt gttggagcgc aaaggtgtgc cttcaaaaat cgaatcgctt
      721 gcgcgcgagc tgtcgattac ggaagacgag tatgtctttt tcgaacgccg tctgaaggcg
      781 atggcgcggg acggtcaggt tttaatcaac cgtcggggcg cggtttgcgc ggcggacaaa
      841 ttggatttgg tcaaatgccg cgtcgaggcg cataaggacg gtttcggttt tgccgtgccg
      901 ctcacgcccg ccaaagacgg tgattttgtt ttgtatgaac gccagatgcg cggcattatg
      961 cacggcgata ttgtcactgt tcgtcctgcc ggcatcgacc gtaggggccg ccgcgaaggg
     1021 acagttctgg atattgtcga acgcgcgcaa agcaaagtgg tcggccgttt ctatatggat
     1081 aggggcgtgg cgattttgga gccggaagac aagcgtctga accaaagcat cgtattggaa
     1141 ccggacggcg tggcgcgttt caaacctgaa tccggtcagg tcatcgtcgg cgaaattgag
     1201 gtttatcctg agcaaaaccg gcaggcagtg gcaaaaatca tcgaagtttt gggcgattat
     1261 gccgacagcg gcatggagat tgaaattgcc gtgcgcaagc atcatttgcc gcaccaattc
     1321 agtgaagcgt gtgccaaagc cgtgaaaaaa attcccgacc atgtacgcaa aagcgatttg
     1381 aaaggccgcg tcgatttgcg cgacctgcct ttggtaacga ttgacggcga aacggcgcgc
     1441 gattttgacg atgcggtgtt tgccgaaaaa atcggacgca attaccgtct ggtcgtggcg
     1501 attgccgatg tcagccatta tgtccgcccc gatgacgcta tcgacacgga cgctcaggaa
     1561 cgcagcacca gtgtttactt cccgcgccgc gtgattccca tgttgccgga aaacctgtcc
     1621 aacggcatct gctcgctcaa tcctaatgtc gagcgtttgt gtatggtgtg cgatatggtc
     1681 atcacttacg cgggcaatat taaagaatac cgcttctacc ccgccgtgat gcgctctcat
     1741 gcccgcctga cctacaacca agtttggaaa tggctttcag gcggcatcgg gcatccgttc
     1801 aaaacacaaa tcgacacgct ttacaaactc ttcaaaatcc ttcagaaaaa gcgtttcgaa
     1861 cgcggggcgg tggagtttga cagcatcgaa acccaaatgc ttttcgacga caacggtaaa
     1921 attgaaaaaa tcgtccccgt tgtccgcaac gatgcccaca agctgattga agaatgtatg
     1981 ctggcggcga atgtttgcgc ggcggatttt ctgttgaaaa acaagcatac ggctttgttc
     2041 cgcaaccatt tgggccccac gcccgaaaaa ctcgccaccc tgcgcgagca gctcggtctg
     2101 ttggggcttc aacttggcgg cggcgacaac ccgtcgccga aagactatgc cgcgcttgtc
     2161 gaacaattca aaggcagacc tgatgccgaa ttgctgcaag tcatgatgtt gcgctccatg
     2221 caacaggcgg tttacgaacc gcattgcgac ggacactttg gtcttgccta cgaagcatac
     2281 gcccacttca cctcgcccat ccgccgctat cccgacctga ccgtacaccg cgccatcaaa
     2341 gccgtgttga atcagcaaac ctacacgcca aaaaaaagct ggcaggcttt gggcgtgcat
     2401 acctcgttct gtgagcgccg tgccgacgac gccagccgcg acgtggaaaa ctggctgaaa
     2461 acctattata tgcgcgataa ggtcggcgaa gtattcgaag gtaaaatctc cggcatgacc
     2521 agttttggta tctttgtaac actggacggc atccacattg acggcttggt gcatatcagc
     2581 gatttgggcg aagactattt caacttccgc cccgaaatca tggcaatcga aggcgaacgc
     2641 agcggcatcc gtttcaacat gggggacagg gttgccgtcc gggtcgcccg tgccgatttg
     2701 gatgacggaa aaatcgattt tgtcctgatt gccgggggga gcggcagggg gcggaaagtt
     2761 aaatcatccg cgtctgccaa accggcaggg acggcgggga aagggaagcc gaaaaccgcc
     2821 gccgagaaaa aaacagcccg aggcggcaaa gtaaggggaa ggggcgcgtc tgccgccgca
     2881 gaatcgggga aaaaggcaaa gaaaccggtt ccgattaagg taaaa
//
LOCUS       DAXIHS010000139         2910 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXIHS010000139 DAXIHS010000000
VERSION     DAXIHS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.139"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            43..1260
                     /locus_tag="ACFOF2_001985"
     CDS             43..1260
                     /locus_tag="ACFOF2_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC7108228.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1257..1388
                     /locus_tag="ACFOF2_001986"
     CDS             1257..1388
                     /locus_tag="ACFOF2_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108229.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1463..1603)
                     /locus_tag="ACFOF2_001987"
     CDS             complement(1463..1603)
                     /locus_tag="ACFOF2_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108230.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1616..2740
                     /locus_tag="ACFOF2_001988"
     CDS             1616..2740
                     /locus_tag="ACFOF2_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC7108231.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGDLERLAARDIIRFPGFVNNVSEVIKAHH
                     IFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKM
                     IYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          684 a          869 c          683 g          674 t
ORIGIN      
        1 cactataaaa tccgcaaact taaccgactc aaaatacaca ttatgacgca caccgcatcg
       61 aaaacaccca aattttggct gaccatcacc gccgccgcat tcatcctgct gattaccatc
      121 ggcatgagga tgacgctcgg actgttcgtc caacccgtcg tcaacaccac cgaattgagc
      181 attgcccaat tcagcctcat catcgccgtt ttccaactga tgtggggcat atcgcaacca
      241 ttgtccggcg cgcttgccga ccgtttcggc gcgttcaggg tattaagcgg cggcgccgtc
      301 ctgctcgtct gcgcctgcct gattgccccc aacatcccga cttattgggg gctgatgatt
      361 gccgtcgggc tgctgctcgc attcggcacc ggttccggcg gtttctccat cattatgggg
      421 caggtcgccg cccaagtccc cgcacacaaa cgcggcttgg catccggact ggtcaacgcc
      481 ggcggttcgg caggacaatt cctgttcgca ccgctggttc aaggactcgt cgtcctgccc
      541 gaagtcggct ggacgggcac attttacgtt tggggcgcaa tcgccctgct gattctgcct
      601 gtctcatggt ggcttgccgg cggcaacaac gccgcccaca cccaacacgc ccaagccaca
      661 cacggacaaa gcctcggaga agcagtcaaa accgccttca aaaccccaag ctacatcctg
      721 ctgcacctaa gcttttttgc ctgcggcttc cacatcgcct ttctcgtaac ccacctaccc
      781 acggaagtcg ccctgtgcgg actgcccgcc accgtcgcct cgacatccat cgccatcatc
      841 ggactggcaa acatcgccgg ctgcattttt tccggctggt gcacaagccg cgttcggggc
      901 aaatacgtcc tgttcggact gtacgcctcg cgcgccgcga tgatactcat ctacattttt
      961 tcgcccaaga ccgacctcaa cttctacatt ttcgccgccg cactcggatt tacctggctc
     1021 gccaccgtca cgccgaccgc ctccattacc ggcaaactct tcggcacacg ctacctcgcc
     1081 accctgttcg gactgacgat gctcagccac caaatcggcg gattcctcgg ctcgtacatc
     1141 ggcggcatcg tgattacaca attcggcgac tacggctgga tgtggtacgc cgacgcactg
     1201 ctggcgggaa ccgccgccct cctcaacctc cccatccgcg aaccccgaac cgccgcatga
     1261 aagaatgccg tctgaaaacc ctcagacggc attttccaaa caccccgccc gtttccggac
     1321 tgcacgacac gcaaatcagc cttatcaaac cgcaaacaac cgccgcgatt aaaccgccat
     1381 tcaaataagt ccgttttccc ttcgggtgta aaatcatcac aaaacgcgat actgtatatc
     1441 gccgggcgtt atgcagcgga tttcaggata aaatgccgtc tgaagccgcc cctgcccggg
     1501 cgggcgaagc gtcggatgcg gtgcgcttgg caaaggtttg cccgccgcgc cccgtatccg
     1561 gcatttcatc aatgcagacc gcatttccaa tcctataatt caaggtgtcc gccttatgaa
     1621 aatcgttttt atcacaacag tcgcatccag catttacggt ttccgcgccc ccgtcattaa
     1681 aaaattaatc ggcaaaaacc atcaggtgta tgcctttgta tcggagtttt ccgataatga
     1741 attggatatt atcagggaaa tgggggtaac acccgttacc taccgttcaa accgcagcgg
     1801 gctgaacccg ttttccgata taaaatccac cttcctcatc tttaaagaac tcaaaaaaat
     1861 atcgccggat ttggttttcc cttatttcgc aaaacccgtg attttcggca cttttgccgc
     1921 aaaactggca ggcgtgccca gaatcgtcgg gatgctggaa ggtttgggat tcgcatttac
     1981 cccgcagccg gaaggcatac cgttaaaaac aaaaatcata aaggggattt tgattgcctt
     2041 gtaccacatt gccctgccga tgttggaaag cctgattgta ttaaaccccg acgacaaaga
     2101 cgaactgacg gacaaatacg gcatcaaaat aaaaaacatc catattttgg gcggaatcgg
     2161 tctggattcg cggcaatatc cttattccga ggcggatatt cccgatgaaa aagaacccgt
     2221 aaaattcctc tttatcggca gatttctgaa agaaaagggg attgatgatt ttattcgggc
     2281 ggcggaacag gttaaggaca aataccccga tacggttttt accgctttgg gcgcaatcga
     2341 caaatcacgc ggggggggcg atttggaacg gcttgccgcc cgcgatatta tccgtttccc
     2401 cggttttgtg aacaatgttt ccgaagtgat aaaggcgcat catatattcg tattgccgtc
     2461 ttatagggaa ggcgttcccc gaagcactca ggaggcaatg gccgtcggca gggcagtgat
     2521 tacgacggat gtccccggat gcagggaaac ggtcgccgac aaggtcaacg gcttcctgat
     2581 cgagccttgg aatccccgca tcttagccga aaaaatgatt tattttatcg aaaacaggga
     2641 agccgtccgc ctgatgggga atgcaagtta tgcgattgcc aaagataaat tcgatgccga
     2701 aaaagtcgat ttgaaattgc tcgatatttt gaaggcgtaa acaaggctgc ccgcttttga
     2761 gtttcggtca tttctgataa acccccgtca ttcccgccac acccccgtca ttccctcgaa
     2821 agcgggaatt caggttcgtt tagtttcggt catttccgat aaattcttgc aactttgcgt
     2881 ttctagattc ccactttcgt gggaatgacg
//
LOCUS       DAXIHS010000140         2750 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXIHS010000140 DAXIHS010000000
VERSION     DAXIHS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.140"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            84..443
                     /locus_tag="ACFOF2_001989"
     CDS             84..443
                     /locus_tag="ACFOF2_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC7108232.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGFSLIYNNLQTKRLI"
     gene            440..1096
                     /locus_tag="ACFOF2_001990"
     CDS             440..1096
                     /locus_tag="ACFOF2_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC7108233.1"
                     /translation="MILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(1075..1209)
                     /locus_tag="ACFOF2_001991"
     CDS             complement(1075..1209)
                     /locus_tag="ACFOF2_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108234.1"
                     /translation="MPSEAGFVASDGIAAILAGRGLFVPTSRALLIWGLPEAYSEKNF
                     "
     gene            1318..2655
                     /gene="ribBA"
                     /locus_tag="ACFOF2_001992"
     CDS             1318..2655
                     /gene="ribBA"
                     /locus_tag="ACFOF2_001992"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC7108235.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
BASE COUNT          738 a          784 c          628 g          600 t
ORIGIN      
        1 gttacagggt gcggatgcaa acgcattgcg agcgcgggtt tgaggcatac gcgcaaacat
       61 cttaatataa tggattgata tttatgattt tctccatcat cgtccctatt tacaatgtgg
      121 aaaaatacct ccgctgctgc gtggattccg tgcttgccga aaattttgcc gattatgaaa
      181 tgattttggt cgatgacggt tcgccggacg gctgcgggaa gatttgcgac gaatatgcag
      241 gcaaatatcc gcatataaaa gtgattcatc aagaaaacgg cgggctgtcg gatgcccgca
      301 acgccggtat ccgggcggca aaaggcgatt acctaatctt tttggacagc gacgactatt
      361 gggccgatac caaccgttca aaaaacgcgg gggggggatt ctctttgatt tacaacaact
      421 tgcagacaaa aaggttgatt tgatcctgca tccctcgtcc ctcaattacc acgtcatccc
      481 caaaggggcg gacttttcgg ataatgattt tgtccgccat tttgaaacgc tggtggaggg
      541 gcggtactat atcgccaacg cgtggacaaa aatcgtcagg cgggaaataa tcatcaaaaa
      601 caatctgttt ttcccaaaag gatacattca cgaggatttc ccgtacagtt tgcaattggc
      661 gcgttttatc aagacttttg ccttttacga taaccctttt taccagtacc gcgttctcgg
      721 cggctctatc agccacaaca tcaaatacaa aaatttcagc gatgtgctga cgcatctcga
      781 ccggggtgtg gattttttag tcgaaaacaa aaattccccc atctacggcg gtttgcaaaa
      841 atttgtcttc gacaatatcg gctatctgag gtctatattg gtaaggcttt atttttccaa
      901 aaacattatc ctcatctacc ggaaatattt ttcatttaaa gaaaaatgca gaaagatatt
      961 cggcgcgaag acaatccgtc cggtttttat cgggaagacc gcattcatca taggattgcc
     1021 gatattgcgc ctgctcgtac cgcctatgct gtacccggca atcaaggccg tttatcagaa
     1081 atttttttcg gaataagctt ccggcaaacc ccaaatcaga agtgcgcggg aagttgggac
     1141 aaacagcccc cggccggcaa ggattgcggc aatgccgtct gaagccacga atccggcttc
     1201 agacggcatc tgtttaccaa aaagcaaata attcggtttg gcgaaaaaaa cagatttgct
     1261 ttttggtaaa tacgcgatta caatccgcta catccgattt ctacaaagga tgaaacgatg
     1321 accgacacag ccggtctgcg ccgccgcaac ctgcggcaat ggatagcaga acactacggc
     1381 ggtttgcaaa cccgttttgc cgaagccgtt gccctcaaca caggcgaact ctccgccctt
     1441 ttgaaaaaca aatccttcgg cgagaaaaaa gcccgtaaaa tcgaacaggc ggcaaaaatg
     1501 cccgccttct ggctcgatac cgaacacacc gcccgcccgc ccgaacacac aggaaaacac
     1561 accatgtccc atatttcccc catccccgaa atcctagccg acatcaaagc cggcaaaatg
     1621 gtcatcatca ccgatgccga agaccgagaa aacgaaggcg acctgctgat ggcggcgcaa
     1681 ttcgtcacgc ccgaagccat caacttcatg atcaaacacg cgcgcggctt ggtctgcctg
     1741 ccgatggacg gcgaaatggt cgaaaaactc gggctgccga tgatgaccca aaaaaacggc
     1801 gcgcaatacg gcaccaactt taccgtctcc atcgaagccg cacacggcat taccaccggc
     1861 atttccgccg ccgaccgcgc cctgactatt caaaccgccg tttccccgac cgctaaaccc
     1921 gaagacatcg tccaacccgg tcatatcttt ccgcttcgcg cccaaaaagg cggcgtactc
     1981 gtccgcgccg gacacaccga agccggcgtc gacctggcgc aaatgaacgg gctgattcct
     2041 gcctccgtta tttgcgaaat catcaacgac gacggcacga tggcgcgtat gcccgaactg
     2101 atgaaattcg ccgaagaaca caagctcaaa atcggcacga ttgccgacct catcgaatac
     2161 cgcagccgta ccgaaagcct gcttgaagac atgggcaatg cgcctgtaca aaccccgtgg
     2221 ggcgagttcc aacaacacgt ttacgtcgac aaactctccg gcgaaaccca cctcgccctc
     2281 gtcaaaggca cgccctccgc cgacaccgaa accctcgtcc gcgtccacga acccttcagc
     2341 gtcatggact tcatccaagc caacccgcgc cattcatggt cgctgcccaa agcactcgag
     2401 cgcatccaac aggccgaaag cggcgtagtc atccttctgc accgcaccga agacggcgcc
     2461 gccctgctcg accgcaccct gcccaaaggt gcaaaccaag cctacaaatg ggacagcaaa
     2521 agctacggca tcggcgcaca aatcctcgtc ggcctcaacg tcaaaaaact gcgcgtcctc
     2581 gggcagccct catctttcac cggcctgacc ggcttcggtt tggaagtcgt cggctttgaa
     2641 gaagcggaaa aataatatag taaattcaaa tactttatat ttgctttatt tattgcatta
     2701 tttccgtgca aacgaaaacc cggtctgttg ggttggattt tgtttttgca
//
LOCUS       DAXIHS010000141         2685 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXIHS010000141 DAXIHS010000000
VERSION     DAXIHS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.141"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..958)
                     /locus_tag="ACFOF2_001993"
     CDS             complement(<1..958)
                     /locus_tag="ACFOF2_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC7108236.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQTVKDVNWNQVQLAYD"
     gene            complement(1103..1534)
                     /locus_tag="ACFOF2_001994"
     CDS             complement(1103..1534)
                     /locus_tag="ACFOF2_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108237.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(1550..>2685)
                     /locus_tag="ACFOF2_001995"
     CDS             complement(1550..>2685)
                     /locus_tag="ACFOF2_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC7108238.1"
                     /translation="EIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVN
                     AAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAAS
                     IAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQ
                     SYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKY
                     PEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGIT
                     RIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIA
                     QNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          639 a          532 c          573 g          941 t
ORIGIN      
        1 tgtcgtaagc gagctgtact tggttccagt tcacgtcctt gaccgtctga agctgtttca
       61 gataagcata ctcgggctgt ttggccagct tttcgatttc ggttttcaga ttgcctttgg
      121 gaatatcgac aatgtaaccg ccgggtgcgg acagtacggg cgcaacggga ccggtgaaac
      181 tcggcaattg caaggtttcg atgttactgc cccgtcctgc ctgtttttgc catagagcag
      241 atttgctgct gctcacggtt tcgttgtgga tcgtggtttt gatgccttcg aggataatct
      301 tggcatctgc ccgtgcctgc tcacctacgc ctgcgcgtat ggttgcgcca cccagtgtgg
      361 tttcaaactg tgtgccttgc agtttggtat cccagcccga ttggaggttg gctgattctg
      421 caactaccct tgagggcagc gcggttttca tgacttgggt ggtggtgtcg tgtgctttgc
      481 tgtagctgat gccgagaaat ctgcgccttt tttggctgtc tagtttgtcg tagttgagct
      541 cttctacggc atagagtgtc aatttccgcc cggcttcgat gttaatgctg cctttggggg
      601 catcgaatgc ggtggcgtag gcgtcgatgc tgccaccaga tttgatgtcg atgccttggg
      661 atgcgctgag gtttactgcg tcgggcttgg cgtttttgtg ttctttgact tcggtaatgt
      721 gtttgcggtt gtaccatttg ccagttttgt agctgcggcg ttcaaaggta tagagttcgc
      781 tctgtctcgc atagtaatat tggtcaccgt aagattgaat tttgattttg ccgttttccg
      841 aactgatatc cgtggtgctg agcaggatgc gactgttctc attggcatac ggtgcgctaa
      901 tgctcacacc ggttttaccc gaaagttctg cagcaagcgt agggggtgtc ttcagcacgc
      961 acgcgtctgg gcagtatata gggaatctga atatttactt gcataacaaa tgccgtctga
     1021 aaaattgtga gcttttcaga cggcattgag ccgtaaatca tggaacgcgt gcgtgctgaa
     1081 gcacacacct tacgcatgga ttttaggttt catgcaggct acagtttgtt tgagaatatg
     1141 ttttttgtat ttctttacac tttttttgat attcagggtg agctattaag aaatcagata
     1201 ttgcaatatt aaagtaatga tatgctaaat taataggtat aattaattct tcaggattga
     1261 aatcatctcc aactgaaaaa taaatagaag gagctttatc aatatactca aatccattta
     1321 cctcttcata atctccaatc catctggtag catctatttt ttcatattga gcacctatta
     1381 ctccaaaatg tccggtagtt gtaatatttt taaaacactc taaaagcgta tctttagttt
     1441 caaattggta aattgtttca aaaaaaatcg aaataacaaa atggttgtta tttatagatt
     1501 ttttatttaa gtttagaaaa atattatttt tcataatttt tcctttaaat tattctgggt
     1561 gaatgtttgt aattcttcct gtatttacat caaaatatga tctaaatttg attccgtcaa
     1621 aggtttctga gaattgaatg acattttttc tttccgatat tgatttagtt ctttcatttt
     1681 gagcaatttt agaggctttt gaatatcctt gtgaagcagc attttgagcc atttgaagta
     1741 ttttatcatc agaaaatttt ttaggattat aaacagtttt tatacttgaa atttccttaa
     1801 atccaccatc aggtttacct gtcctgtcta gtgtaggaat ctcatattta attcgggtaa
     1861 tgccttcaat atcagtttgg gtttcagatt ttacgcgtcc tcctcgtgaa tttagttctg
     1921 ccataaaatt ggtgcggtta tgggctcctt taatgccctg tttttgacta aaaccatcag
     1981 catttgccag atgtcttttt aggtttgtat cgaaactgat gccttccggg tatttgacat
     2041 tagtaatagg tgtattttgt tttacaaacc ctcttggtat tggaatgaat ctaggattaa
     2101 ctcctgaaat aagtgtataa ccttggtcgg acatccattc ggataaaggc tttgtattgc
     2161 ccgattgaag ccaagattta gcagcagtat tcaaatgata agactgtaaa gccaaatctg
     2221 cttgggtgta agatttgctg aataatttat accattcatc atctttacca attagacctg
     2281 cttcccaaga tgaatgaagg cttctactat cgatcaaatg ttgttttctg attgttctac
     2341 attcagtact acgggatata tccgtacata ttgcaatcga agcagcaagt cttttatcag
     2401 caacattttg atactttttt acagtatttt ttctgcacag ttgaggatta ttctgtttgg
     2461 cgcatgcagt catttcgtta tcaaattctc taccctcttt gtcgctaagc tgattatttt
     2521 tcaccgctac ctcagccgca ttcgccgccg catttacatc gccgccgacc acaccgctta
     2581 ccgtaccggc aaccagtttg ctgtatgcca aaatctgttc gcgttcttta gctgtcaaag
     2641 tgtcaggatt tttgccgttt gtcaaagcct ccccgactat ctcgc
//
LOCUS       DAXIHS010000142         2642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXIHS010000142 DAXIHS010000000
VERSION     DAXIHS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.142"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(17..454)
                     /gene="rnhA"
                     /locus_tag="ACFOF2_001996"
     CDS             complement(17..454)
                     /gene="rnhA"
                     /locus_tag="ACFOF2_001996"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC7108239.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            568..1437
                     /gene="tehB"
                     /locus_tag="ACFOF2_001997"
     CDS             568..1437
                     /gene="tehB"
                     /locus_tag="ACFOF2_001997"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC7108240.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAESLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPEVIADMQAHTAVG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVKYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1487..2293)
                     /gene="thiD"
                     /locus_tag="ACFOF2_001998"
     CDS             complement(1487..2293)
                     /gene="thiD"
                     /locus_tag="ACFOF2_001998"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC7108241.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETIFAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          558 a          646 c          789 g          649 t
ORIGIN      
        1 tttgccggag cggcagtcag gaaaactgcg ctgcgccacg gtttgccaaa tcgtcggcgc
       61 gttcgttttc cgcgtgtccc gcgtgtcctt taacccaagt ccagctgact tggtgctgcc
      121 caaccagaac gtcgagttct ttccacaagt cgtcgttttt gacgggctgt ttggcggcgg
      181 ttttccagcc gttgcgcttc caaccgtgta tccagttttc catgccgttt ttgacgtatt
      241 gcgagtcggt gcagatgatg acggtgcagc ggcgtttgag cgatttcagt ccttcgatga
      301 cggcggtcag ctccatgcgg ttgttggtgg tttgcgcttc gccgccgaaa agttcttttt
      361 cgtggctgcc gtagcgcatc aacacgcccc agccgcccgc gccgggattg cctttgcacg
      421 cgccgtcggt gtaaaggtaa acggtttggt ccattattgt gcctttgtgt ggtgtccggc
      481 gtgattataa gcggtttgtg cgccgtgccg tctgaaagat tgcggcggat taaaataggc
      541 atataatgtg caactttatt tttgaatatg aaggagcgga tagtgggtca aagcggcgag
      601 ttgttttgtt tcggacaaat gcccgtatgg aaggcggaaa acctgccgga aattttgttg
      661 tcgggttatt cgtccgaagg aggggaatgg gtctgcctga atgtgttgca gggcgatgtc
      721 gaagtccgca cgcaggacgg ggcggcagag gtttggtcgg cggaaagcgg cgattgcgtg
      781 tttgcgccgc agcaggtgtt ttcggtcaaa ctgaaaacgg acgatgccga aatccgtttg
      841 tcgctgtatt gcgcggcggc agactatttt cacaaaaaat acgggatgag tgccacgcat
      901 tctgcggtcg cggcggcacg ggataccgta ccggcgggca gggcgttgga tatgggctgc
      961 ggacaggggc gcaacgcgct gtttctcggt ttgaaggggt ttgaagttac tgcagtcgat
     1021 cacaatcccg ccgccttggc aaacgtggcg gagctggcag aggcggagag tttgaacgtc
     1081 cgcacgctgg aatatgattt gaacgccgcc gccttgcagg gcgagtttga ttatattgtg
     1141 gcaacggtgg tgctgatgtt cctgatgccg cagcgcgtgc ctgaagtgat tgccgatatg
     1201 caggcgcata cggcggtggg cgggtacaac ttaatcgtat cggcaatgga tacggcggat
     1261 ttcccctgtc cgatgccgtt ccccttcaaa tttaaagagg gagagctgaa ggattattat
     1321 cgggattggg agctggtcaa atataaagaa gaattgggcg cgatgcacgc caaagacgaa
     1381 aacggcaatc cgatacggtt taaatttgta acgatgctgg cgaaaaagcc tgaataatcc
     1441 tcaaaaatgc cgtctgaaca ttttgtttca gacggcattt ttacgtctag tcccgatacg
     1501 cccaatgatt gaccggcccg tgtcctgcgc cgatttccaa agggtttgag attgccgccg
     1561 tgatgtatgc cttggcagtc tgtacggctt tgcaaacgtc taagcctttt gccaactcgg
     1621 cggtaatgca ggcagaaaac gtgcagcccg tgccgtgcgt gtgggcggtt ggaaagcgcg
     1681 ggctgtccaa ttccagcgtt tcattttgcg taaacagcca atccgtgcag cgtccgcttg
     1741 tgctgccgtt caaatgtccg cctttgataa cgacattttt gacaccgtaa tcaagcagga
     1801 tttttgccgc acgttccgca tctttacggt tttcgatatg cacgccggtc agggcttccg
     1861 cctcgggcag gttgggggtc aatacatccg tatcgggaag cagcaggcgc gtcagtgccg
     1921 caacggcgga atcctgcaac agcggcgcac cgcctttggc aatcatcacg gggtcgagta
     1981 cgcgcctgcc gaagtcgcag tgtttcagtt tgtcggcaac gcattcgatg atttccgccg
     2041 tgccgagcat tcctattttg taggcgcgga tgtcaaagtc ttccctgatt gcttggattt
     2101 gtgcgaagat ggtttcggtc gggacgagat gaaccgccga cacgcccaag gtattttgcg
     2161 cggtaacggc ggtgatgacg cacgttccga acacgccgcg catctgaaac gttttcaaat
     2221 ccgcctgaat gcccgcaccg ccgcccgaat ccgaaccggc gatggtaagc gtttgcacaa
     2281 aagagctttt catttgttcc tccttaatat tgtgtccgat aaaaagggct aatgcctgta
     2341 aaaaagcacg gcgatgccgt ctgaaaaccg cagcgcgggg tttgtggtgc attatatgcc
     2401 gtattgcagg acaaggcaca gggcagggcg gcggggtgca tcgtagcaac tgtatttttg
     2461 cccgacgggg tgaaaaatac agttgctaca cccactgata atgaaaaaag agagcaatgc
     2521 cgtctgaacc gcgtgttgcg gcgagcataa accggcacgc tttattcgtc attcccacaa
     2581 aaacagaaac ctaaaattcc gtcattccta cgaaagcggg aatccagtgt gttcggtttc
     2641 ag
//
LOCUS       DAXIHS010000143         2611 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXIHS010000143 DAXIHS010000000
VERSION     DAXIHS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.143"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            388..900
                     /locus_tag="ACFOF2_001999"
     CDS             388..900
                     /locus_tag="ACFOF2_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC7108242.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            980..2059
                     /gene="aroB"
                     /locus_tag="ACFOF2_002000"
     CDS             980..2059
                     /gene="aroB"
                     /locus_tag="ACFOF2_002000"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC7108243.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          644 a          776 c          624 g          567 t
ORIGIN      
        1 cgattcactt ggtgtttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa
       61 cgctgtactg gtttttgtta atccactata gtattgataa acatattatc ttcaatatat
      121 tcaattggat aattgtttac ctaagcaaag ataattgcct tttcctgaca aataagtgaa
      181 atcaacggat tgtcaaaaca cagcctgaaa taaaaaacct ccctgatttc ttttattttt
      241 ccttaaaatc agaaaggttc gggatggtcg ggttattttt ccaaacgtac cgctgccctg
      301 ccgatttcgt ataaaattcc gccgtaaccc gacaagcccg aaccctgtcg ccccgaaagg
      361 cggggtgtca aacattaagg aattgtgatg aaaaacttta acggcaaact catcctcatc
      421 ggactgatgg gcgcgggcaa aaccacgctg ggccggcaaa tggcgcagcg gctggattac
      481 cgtttttacg acagcgatca cgaaatcgcc gtagccgccg gcgttcccat ccccaccata
      541 tttgaaatgg aaggcgaaca gggattccgt tcgcgcgaaa ccgccatact caaaaagctg
      601 gttatcctgc cccatatcgt cctgtccacc ggcggcggcg cggtgttaaa agaagaaaac
      661 cgcgccctta tccgcaaaag cggcacggtc gtctatctgc acgccccgcc cgaaaccctg
      721 ctcgaacgca cgcgctgcga caacagccgt cctttgctgc aagttgccga tcctttggcg
      781 aaattacgtg aactctacgc cgcacgcgac cccgtttacc gccaaaccgc cgactttacc
      841 gtagaatcgg caaactgccg ggaaaccgtg caaaccctgc tcaaacgctt atcccgataa
      901 accggcatat gcgccgcgcc cagaaaacca agccgcgccc cgcccggcgg gccggcggtt
      961 caaactttaa ggaacaacaa tgaaaacact gaccgtacac accccgtccc acagctaccc
     1021 catctttatc ggcaacgggc tgctgccgca ggcaggaagc ctgctcaaac cgcatttggg
     1081 caaacgcgcc gccatcatcg ccaacgaaac cgtcgccccg ctctacctcg gcacgcttca
     1141 gacggcattg gatgcggcag gcgtatccca tttcagcatc atcctgcccg acggcgaggc
     1201 gcacaaaaac tggcagacgc tcaacctcat ctttgacggg ctgatgcaaa accgcgccga
     1261 acgcaaaacc acattaatcg cactgggcgg cggcgtgatc ggcgacatgg tcggctttgc
     1321 cgctgccacc taccagcgcg gcgcaccgtt cgttcaaata ccgaccacac tgttgagtca
     1381 agtcgactca tcggtgggcg gcaaaaccgc catcaaccac ccgctcggca aaaatatgat
     1441 cggcgcattc taccagccgc aggcggtgct tgccgacttg gacacgctgc acaccctgcc
     1501 cgtccgcgaa ttgtccgccg gtatggcgga agtcatcaaa tacggcgcgc tcggcgacat
     1561 cggttttttc gaatggctcg aacaaaacat atccggactg atgacgctcg atcgggaaaa
     1621 actcgcccaa gccgtgtacc gctgctgcca aatgaaggca gacatcgtcg cccaagacga
     1681 aaccgaacag ggcatacgcg catggctcaa cctcggacac accttcggac acgccattga
     1741 aaccgagatg ggttacggca cttggctgca tggagaagcc atcgccgccg gctgcgtgtt
     1801 ggcggcgcgt ttgtccgaac aactgggcaa aacctccgcc gcagataccg cgcggctcgc
     1861 cgccctgctc gaagccgccg gactgccgtc cgcaccgccc gtgtttgcct ttgaaaaatg
     1921 gctcgaacat atgagccacg ataaaaaagt cagcggcggc atcatgcgct ttatcggtct
     1981 gaaccggctg ggcgaagcca acatcaccga aattaccgac acggacatcc tccgccgcac
     2041 cctgcaaccg tatctctgat ttcctctgcc gatgtgctgc cgcgcgggtt tgacgcacga
     2101 tgatgatttt ccatcatctt tctccgtaaa agcgggaatc cggtccgttc ggtttcggtc
     2161 gtttccgata aattcctgct gcttttcatt tctagattcc cactttcgtg ggaatgacgg
     2221 cggagaggtt tttgttgttt cggagaagtt tctgcaaccc tagaatctcg ttatttccac
     2281 aaaaaacaga aacccaaaac agcaacttaa aacctcgtca ttcccgcaaa agcgggaatc
     2341 cggtccattc agtttcggtc gtttccgata aattcctgct gcttttcatt tctagattcc
     2401 cactttcgtg agaatgacgg cggaagggtt ttggtttttt ccgataaatt cttgaggcat
     2461 tgaaattcta tagtggattc acaaaaatca ggacaaggcg acgaagccgc agacaataca
     2521 gatggtacgg aaccgattca ctcgtgcttc agcaccttag agaatcgttc tctttgagct
     2581 aaggcgagac aacgccgtac cggtttttgt t
//
LOCUS       DAXIHS010000144         2486 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXIHS010000144 DAXIHS010000000
VERSION     DAXIHS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.144"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            256..2421
                     /gene="fetA"
                     /locus_tag="ACFOF2_002001"
                     /gene_synonym="frpB"
     CDS             256..2421
                     /gene="fetA"
                     /locus_tag="ACFOF2_002001"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC7108244.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKGADGKEVD
                     VDDAQKEKNRANEKIVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          709 a          716 c          570 g          491 t
ORIGIN      
        1 cgcgggaagg acgcggaaag gttgcttttc gtcattcccg catttgttaa tccactatat
       61 ttccgccgtt ttttacattt ccgacaaaac ttgtcaacaa aaaacaacac ttcgcaaata
      121 aaaacgataa tcagctttgc aaaaatcccc ccccctgtta atataaataa aaataattat
      181 taattatttt tcttatcctg ccaaacctta acggtttgga tttacttccc ttcatacact
      241 caaaaggacg aacaaatgaa cgccccgttt ttccgcctca gcctgctctc gcttaccctg
      301 gcggcaggtt ttgcccatgc ggcagaaaat aatgccaatg tcgcattgga taccgttacc
      361 gtaaaaggcg accgccaagg cagcaaaatc cgtaccaaca tcgttacgct tcaacaaaaa
      421 gacgaaagca ccgcaaccga tatgcgcgaa ctcttaaaag aagagccgtc catcgatttc
      481 ggcggcggca acggcacgtc ccaattcctg acgctgcgcg gtatgggtca gaactctgtc
      541 gacatcaagg tggacaacgc ctattccgac agccaaatcc tttaccacca aggcagattt
      601 attgtcgatc ccgctttggt taaagtcgtt tccgtacaaa aaggcgcggg ttccgcctct
      661 gccggtatcg gcgcgaccaa cggcgcgatt atcgccaaaa ccgtcgatgc ccaagacctg
      721 ctcaaaggct tggataaaaa ctggggcgtg cgcctcaaca gcggctttgc cagcaacgaa
      781 ggcgtaagct acggcgccag cgtattcgga aaagagggca acttcgacgg tttgttctct
      841 tacaaccgca acgatgaaaa agattacgaa gccggcaaag gtttccgcaa tgtcaacggc
      901 ggcaaaaccg taccgtacag cgcgctggac aaacgcagct acctcgccaa aatcggaaca
      961 actttcggcg acgacgacca ccgcatcgtg ttgagccaca tgaaagacca acaccggggc
     1021 atccgcactg tgcgtgaaga atttaccgtc ggcgacaaaa gttcacggat aaatattgac
     1081 cgccaagccc ctgcttaccg cgaaactacc caatccaaca ccaacttggc gtacacgggt
     1141 aaaaacctgg gctttgtcga aaaactggat gccaacgcct atgtgttgga aaaagaacgc
     1201 tattccgccg atgacagcgg caccggctac gcaggcaatg taaaaggccc caaccatacc
     1261 cgaatcacca ctcgtggtgc gaacttcaac ttcgacagcc gccttgccga acaaaccctg
     1321 ctgaaatacg gtatcaacta ccgccatcag gaaatcaaac cgcaagcgtt tttgaactcg
     1381 aaatttgaga tctccgataa gaaaaaagga gccgacggaa aagaagtcga cgtagatgat
     1441 gcacaaaaag aaaaaaatcg tgccaatgaa aaaattgttc acgcctacaa actgtctaac
     1501 ccgaccaaaa ccgataccgg cgcgtatatc gaagccattc acgagattga cggctttacc
     1561 ctgaccggcg ggctgcgtta cgaccgcttc aaggtgaaaa cccatgacgg caaaaccgtt
     1621 tcaagcagca accttaaccc gagtttcggt gtgatttggc agccgcacga acactggagc
     1681 ttcagcgcga gccacaacta cgccagccgc agcccgcgcc tgtatgacgc gctgcaaacc
     1741 cacggtaaac gcggcatcat ctcgattgcc gacggcacga aagccgaacg cgcgcgcaat
     1801 accgaaatcg gcttcaacta caacgacggc acgtttgccg ccaacggcag ctacttctgg
     1861 caaaccatca aagacgcgct tgccaatccg caaaaccgcc acgtctctgc cgctgtccgt
     1921 gaagccgtca atgccggtta catcaaaaac cacggttacg aattgggcgc gtcctaccgc
     1981 accggcggcc tgactgccaa agtcggcgta agccacagca aaccgcgctt ttacgatacg
     2041 cacaaagaca agctgttgag cgcgaatcct gaatttggcg cacaagtcgg ccgcacttgg
     2101 acagcctccc ttgcctaccg cttcaaaaac ccgaatctgg aaatcggctg gcgcggacgc
     2161 tatgttcaaa aagccgtggg ttcgatattg gtggcaggtc aaaaagaccg cagcggcaaa
     2221 ttggaaaacg ttgtacgcca aggtttcggt gtgaacgatg tcttcgccaa ctggaaaccg
     2281 ctgggcaaag acacgctcaa tgttaatctt tcggttaaca acgtgttcaa cacgttctac
     2341 tatccgcaca gccaacgctg gaccaatacc ctgccgggcg tgggccgcga tgtacgcttg
     2401 ggcgtgaact acaagttcta aaacgcacat cccgaaaaaa tgccgtctga aagcctttca
     2461 gacggcatct gttctgataa tttgat
//
LOCUS       DAXIHS010000145         2484 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXIHS010000145 DAXIHS010000000
VERSION     DAXIHS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.145"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..>2484
                     /gene="pilC"
                     /locus_tag="ACFOF2_002002"
     CDS             <1..>2484
                     /gene="pilC"
                     /locus_tag="ACFOF2_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC7108245.1"
                     /translation="QAQAQTQTQTHKYAIVMNNQKLPEVKWGNDYSSLTDKSNEREVT
                     HTSKFVVAKKHISFSFNNTDEVVAEKKDAVVFGAATYLPPYGKVSGFDTAKLAERKNA
                     LDQIGTTHPGLIGYNYQDVTCASGNCPELSYRTQFTFNNGLKRNGNGGRLDIYEDKSR
                     DSSPIYKLKDYPWLGVSFNLGGESSFKPKRQGSLVSSFSEDVTQQNGTQDQYKSKNLV
                     YTTDDYNSKGNKNHQDKHHAVAFYLNAKLHLLDKKQIKNIAQGVTVDLGTLKTRIEPT
                     DAWKNRQGSYFNNGTWTYEEKGSVSVKLKLPEVKAGRCITTPNPNPKSKAPSPALTAP
                     ALWFGAGQDGKAEMYSASVSTYPDSSSSRIYLQNLKRKTDSNRPGRYSLADLSASDIQ
                     SKEPTFTSRQTIIRLDGGVRHIQLDRNNEVTSFNGDNGTFGIVNEGSVVPESNEWKKV
                     LLPWTVRGFADDSKFKAFNKEKNNDNKPKYSQRYRIRENGNNGKRDLGDIVNSPIVAV
                     GEYLATSANDGMVHIFKKGNGGDARDYSLKLSYIPGTMPRKDIQSQDSTLAKELRAFA
                     EKGYVGDRYGVDGGFVLRQVNNLNGQDRVFMFGAMGFGGRGAYALDLTKADGNDPTKA
                     SLFDVKDNGNNGNNGNNRVELGYTVGTPQIGKTHNGKYAAFLASGYATKEITSGENKT
                     ALYVYDLENNNGTPIAKIEVPNGKGGLSSPTLVDKDLDGTIDIAYAGDRGGKMYRFDL
                     SSQSPDQWTVRPIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDKMDEQYIYG
                     IFDDDTETTGNVKVDLKGLGGGLLEQHLTQ"
BASE COUNT          745 a          602 c          623 g          514 t
ORIGIN      
        1 caggcgcaag cgcaaacgca aacgcaaacg cataaatacg ctattgtaat gaacaatcaa
       61 aagctgcctg aggtaaagtg ggggaacgac tatagctcat tgacggacaa aagcaatgaa
      121 cgcgaagtta cccatacgag taaatttgtt gtagcaaaaa agcacattag tttctcattc
      181 aataataccg atgaagttgt tgctgaaaaa aaagatgctg tcgttttcgg cgcggcgact
      241 tatctgccgc cctacggcaa ggtttccggt tttgataccg ctaagctggc cgagcgcaaa
      301 aatgcccttg atcagattgg tacgacccac cctgggttga taggctacaa ctaccaagat
      361 gtcacttgcg ccagcggaaa ctgtcctgaa cttagctata gaacccaatt taccttcaat
      421 aacgggttga aaagaaatgg aaacggcggc aggctggata tatacgaaga caaaagccgc
      481 gacagttcgc ccatttacaa attgaaggat tatccttggt tgggcgtgtc tttcaatttg
      541 ggcggcgaga gctccttcaa accaaagaga caaggttctt tggtatcttc ttttagcgag
      601 gatgtgacgc agcaaaatgg tacacaagac caatacaaaa gcaaaaacct cgtttatacg
      661 acagacgatt acaatagtaa gggtaataaa aaccatcagg acaaacacca cgccgtcgcc
      721 ttttatctga acgccaagct gcatttgctg gataaaaaac aaatcaaaaa tatcgcgcaa
      781 ggcgtaacag ttgatttggg taccttgaaa acacgcatcg agccgacgga cgcgtggaaa
      841 aacaggcagg ggagctattt taataatggt acttggacgt atgaagagaa aggatcagtc
      901 agcgtcaaac tcaaattgcc ggaagtcaaa gcaggccgct gcatcaccac accgaacccc
      961 aatcccaaat ccaaagcccc ttcgccggca ctgaccgccc ccgcgctgtg gttcggagct
     1021 gggcaagatg gtaaggcgga gatgtattcc gcttcggttt ccacctaccc cgacagttcg
     1081 agcagccgca tctaccttca aaatctgaaa agaaaaaccg actccaacag acccggccgc
     1141 tattccctcg cagacttgag tgcgtcggat attcaaagta aagagccgac tttcacaagt
     1201 cggcaaacaa tcatccgctt ggatggtggc gtacggcata tccaactgga tagaaacaat
     1261 gaggtcacta gttttaatgg cgacaacgga actttcggca ttgttaatga agggagcgtc
     1321 gttcctgaaa gcaacgagtg gaaaaaagtg ctgctgcctt ggacggttcg gggttttgct
     1381 gatgacagta aatttaaagc attcaacaaa gaaaaaaaca acgacaacaa gccaaaatac
     1441 agccaaagat accgcatccg cgaaaacggc aacaacggca agcgcgattt gggcgacatc
     1501 gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta cttccgccaa cgacgggatg
     1561 gtgcatatct tcaaaaaagg caacggcggg gacgcgcgcg actatagtct gaagctcagc
     1621 tacatcccgg gcacgatgcc gcgcaaggat attcaaagtc aagactccac cctcgccaaa
     1681 gagctgcgcg cctttgccga aaaaggctat gtgggcgacc gctacggcgt ggacggcggc
     1741 tttgtcttgc gccaagttaa caacttaaat gggcaagacc gcgtgtttat gttcggcgcg
     1801 atgggctttg gcggcagagg tgcgtatgcc ttggatttga ccaaagccga cggcaatgac
     1861 ccgacaaaag cctctttgtt tgatgtaaaa gataacggca ataatggcaa taacggcaat
     1921 aatcgcgtgg aattaggcta caccgtcggc acgccgcaaa tcggcaaaac ccacaacggc
     1981 aaatacgccg ctttcctcgc ctccggttat gcgactaaag aaattaccag cggcgagaat
     2041 aaaaccgcgc tgtatgtgta tgatttggaa aacaacaacg gtacgccgat tgcaaaaatc
     2101 gaagtaccca acggcaaggg cgggctttcg tcccccacgc tggtggataa agatttggac
     2161 ggcacaatcg atatcgccta tgccggcgat cgcggcggga aaatgtaccg ctttgatttg
     2221 agcagtcaaa gtcctgatca atggactgta cgccctattt ttgaaggcac aaaaccgatt
     2281 acttccgcgc ccgccatttc ccaactgaaa gacaaacgcg tggtcatctt cggcacgggc
     2341 agcgatttga gtgaggatga tgttgataaa atggacgaac aatatattta cggtatcttc
     2401 gacgacgata cggagacgac gggtaatgta aaggtagacc ttaaaggttt gggaggcggg
     2461 ctgctcgagc aacaccttac tcag
//
LOCUS       DAXIHS010000146         2437 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXIHS010000146 DAXIHS010000000
VERSION     DAXIHS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.146"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(2..838)
                     /gene="cysT"
                     /locus_tag="ACFOF2_002003"
     CDS             complement(2..838)
                     /gene="cysT"
                     /locus_tag="ACFOF2_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC7108246.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            977..1084
                     /locus_tag="ACFOF2_002004"
                     /pseudo
     CDS             977..1084
                     /locus_tag="ACFOF2_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1129..1461
                     /locus_tag="ACFOF2_002005"
     CDS             1129..1461
                     /locus_tag="ACFOF2_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108247.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            1790..2299
                     /locus_tag="ACFOF2_002006"
     CDS             1790..2299
                     /locus_tag="ACFOF2_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC7108248.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          671 a          590 c          584 g          592 t
ORIGIN      
        1 ctcaaccctt cgcgcccgaa cgcctgccca acgcccactg catcacgttc agcgcaaaca
       61 gaatcacaaa cgaaaccagc agcataaaca acgccaccgc cgacgcgccc tgtacgtcga
      121 actgttccag cttgcccgta ataatcagcg gcaggatttc agaaaccatc ggaatattgc
      181 ccgcgataaa aatcaccgag ccgtattccc ccgttgcccg cgcgaacatc attcccgcgc
      241 cagtcaagag tgccggcgtg atttccggca gcagcacgcg gcggaacgtc gtaaagcggt
      301 ttgcgcccaa agtcgccgcc gcttcctcat attcgcccga taattcctcc aacaccggct
      361 gcacggcgcg gacgataaag ggcaggctga cgacgaccag cgcaatccaa atgccgacgg
      421 gcgtaaacgc gattttgatg cccaaaggct cgaaaaaacg gcctatccaa ccgttgggcg
      481 catacagggt cgccaacgcg atacccgtaa ccgccgtcgg cagcgcaaac ggcaaatcga
      541 ccagcgcgtt cgccagaccc ttgcccggga attcataacg caccaatacc cacgccacca
      601 gcgtgccgaa cacgacattg gtcagcatcg cataaaacga catccgcaaa ctcagccaca
      661 ccgccgccaa cacgttcggc tcggcaatcg tgttccaaaa gccgccccag ccgatttccg
      721 ccgccttcgc cgccatcatc gcaaacggca agaccacaag cagcgacagg cacaatacgg
      781 tcagaccaag gctgagtttg aagccgggca gtacgccggg cgttttgagc ggtaacataa
      841 aacaatgctg aaaataagga aaaggaagga ctactttaac gatgccgtcc gaaaaacgga
      901 aagaatggaa agtttggtgc aaagacgaat ttgttataaa gcggttggca gtttctcaag
      961 cgggcgcgat gttttaaaat atagtggatt aaatttaaat caggataagg cgacgaagcc
     1021 gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggttta aatttaatcc
     1081 actataacgc cttgttttga cggaaaacca tcatataaag gaacacttat gcagatttta
     1141 tcttttcagg cggacattgc ggaacgtatg ctggaaggta cggaaggcga gtcggtcaac
     1201 gaaaacgcac aattcgtccg tacggacaac ggctattgga ttgcgtggca tgaaggcgta
     1261 gcggcactgc ttgcgcccga tacgccgccg ggcattccct gtttttgggt ggaaggcgcg
     1321 gaaagccttg aagagttgtg cgccatggtg gaacgcggcg agtttgacga agtggaagag
     1381 tttgacggcg atgacgacga atggctcgaa acggcacagg gttgcggaca ccacggcgac
     1441 gcttgcgctt gcggacatta aaggcattgc aggcttgccg caagggggta aggctttgcc
     1501 gttttttaaa taatcggtgg attgcatctg tttgcaatgg gcggaacgct tccctttgta
     1561 ttttattgaa taaaaaaact tatcttagta ttatataaag caatatcaat tattttggga
     1621 tggcaataaa cgcaaagcat tgatttgcgc cgattgcaga cttattatag caggttgcgg
     1681 cgcggactta atgggaattg tttatagagc atgatggtct gaaacatcat taattaaaaa
     1741 taccattaat ccgatttata tttatttcaa tttcaatgga aaaacatcaa tgacaatgat
     1801 tttaagcatt ttaagcctgt tttttatcat cagactgtta tttttagccg tctctattaa
     1861 acatgaaaaa gccttgattg ccaaaggggc gaaacaatac ggaaaaacca attccacgct
     1921 gcttgcggca gttcatacgc tttattattt ggcgtgtttt gtttgggtat ggctttctga
     1981 cactgctttt aatggcatat ccttgattgg tacgctgacg gtgatggctt catttgtgat
     2041 attgtcattg attattaagc agttggggga gatttggacg gttaaaatct atattttacc
     2101 aaatcatcaa attaatcgtt cgtggttgtt taaaacattc cgccacccca attatttttt
     2161 aaacatcata cccgaactga ttggcatcgc cttattatgt caagcgtggt atgttttatt
     2221 gattggcctg ccgatttatt tgctggtctt atttaagcgt atccgacaag aagaacaggc
     2281 gatggcaaca cttttttaac ccgtttcatc aattatagcg gattaacaaa aaccagtacg
     2341 gcgttgcctc gccttgccgt actggttttt gttaatccgc tatattccgc catctctaag
     2401 atttacagcg atacacgggt gatttaagga atgcccg
//
LOCUS       DAXIHS010000147         2415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXIHS010000147 DAXIHS010000000
VERSION     DAXIHS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.147"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..107
                     /locus_tag="ACFOF2_002007"
     CDS             <1..107
                     /locus_tag="ACFOF2_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC7108249.1"
                     /translation="AYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            211..768
                     /locus_tag="ACFOF2_002008"
     CDS             211..768
                     /locus_tag="ACFOF2_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC7108250.1"
                     /translation="MGFGCREGREFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMSLFPQALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGKLGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(855..1823)
                     /gene="ispH"
                     /locus_tag="ACFOF2_002009"
                     /gene_synonym="lytB"
     CDS             complement(855..1823)
                     /gene="ispH"
                     /locus_tag="ACFOF2_002009"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC7108251.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1852..2349)
                     /gene="lspA"
                     /locus_tag="ACFOF2_002010"
     CDS             complement(1852..2349)
                     /gene="lspA"
                     /locus_tag="ACFOF2_002010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC7108252.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          544 a          718 c          621 g          532 t
ORIGIN      
        1 cggcctattt cggactgatt aaagtgagtg cgcaaagcca cctgaaggcg atgtgtttga
       61 acctgttgaa agccgccaac aggctaagtg cgcccgctgc cgcctaaaag gcagcccgga
      121 tgcctgatta tcgggtatcc ggggaggatt aagggggcgt ttgggtagaa ttaggaggta
      181 tttggggcga aaacagccga aaatctgtgt ttgggtttcg gctgtcggga gggaagggaa
      241 ttttgcaaag gtctcagcct gcccgaaatc atccgcgacc tgctcgaaat gccgtctgaa
      301 accgccattg ccgacatcac acgcacttat tccgcacatt acctcaatcc caacaaccgc
      361 aatatgtcct tatttcctca ggcactgccc tgtctggaca agctcaaagc acaaggatac
      421 tggcttgccg tcgccacggg caaagggcgg gcgggtttgg acaacgccat cagccaaacc
      481 gccaccggcg gctattggct cgccaccgcc tgcgcggggg aatatccctc caaaccctcg
      541 cccgaaatgg tattcggaat ctgcggcaaa ctgggacttg acccgaaaga ggcattggtc
      601 gtcggcgata cggcgcacga cctgcatatg gcggcaaacg caggcgcggc ggcagtcggc
      661 gtggccaccg gcgcacattc gcgcgaacag ctccgcaccg caccgcatct cgccgtattg
      721 gacggtttgt ccgaactgcc cggttttctt gcacagcatt acgcctgatt ggtttccgca
      781 tcagacactc aaaacaaatg ccgtctgaag cctgttcaga cggcatttgt gttgcccaaa
      841 cattcaacgc ttgctcaacg tttgcacaaa tcgggtttgg tttcgccctc gcggcgcagc
      901 tctttgggca ggacgaacac aatgctttct tccgcaccct cgccttcgcg tacggtttcg
      961 tgcccccatc cgcgtatggt tgccagtact tcccgcacca acacttcggg cgcggacgcg
     1021 cctgccgtta cgccgacttt gttcttgccc tcaaaccacg cgcgttgcag gtagcctgca
     1081 ttgtccacca tatacgcatc ggttccgatg cctgccgcca cttcgcgcag gcggttgctg
     1141 ttggacgaat tgggcgaacc gaccacaatc acgatgtcgc actgttctgc caactctttg
     1201 acggcggttt gccggttggt cgtcgcatag cagatatctt ccttgtgcgg attgcggata
     1261 ttggggaaac gcgcgttcag cgcggcgatg atgtctttgg tttcatcgac cgagagcgtg
     1321 gtttggctga cataggcgag tttgtcgggg tttctgactt cgagttttgc cacatctccg
     1381 accgtttcga ccaaaagcat tttgcccggc gcaagctgcc ccatcgttcc ttcaacctcg
     1441 acgtgcccct tatgcccgat catgatgatt tcacagtctt gggcatccag tcgggcgact
     1501 tccttatgca ctttcgtcac cagcgggcaa gtcgcatcaa atacgcggaa accgcgctcc
     1561 gccgcttctt gccgcaccgc cttcgatacg ccgtgtgccg aataaaccag tgtcgcgccc
     1621 ggcggcactt ccgccaagtc ttcaataaac accgcacctt tttcacgcag gttgtccacg
     1681 acgaatttgt tgtgaacgac ttcgtggcgc acataaatcg gcgcgccgaa ttcttccaaa
     1741 gcacgttcga caatactgat tgcccgatcc acgccggcgc agaagccgcg cggattggca
     1801 agaatgatgg ttttctcgtt cataagcccg gtatttcgtt ttcagacggc atcaatattt
     1861 ttcttcttgg gttttacggt ggacgatgtt gtccaacact gccaacaccg caccgacgca
     1921 gataaagctg tcggcaatat taaaggcggg ataaaaccaa ttttgccaat aaaacaataa
     1981 gaaatcgacg acatgaccgt gtatcaggcg gtcgatgaca ttgcctaacg caccgccgat
     2041 aatcatcgcc gcacccgttt tgccgagggt tgcaaactca tcgcgcaaga tggcgcgtac
     2101 caaatacgcg ctcaccgcca ccgccagcac caaaaaaaag tatttttgcc agccgccctg
     2161 atcggcaagg aagctgaacg ccgcacccgg gttgtacacc aaggtcaaat cgaaaaagga
     2221 aggaatgaca ttgacgcgtt cccgatactg aaacgacgac agcaccgccc acttcgacca
     2281 ctggtccagc acgatggcgg caagtgccaa tacccaatag cgcgttttac ttgaaacaga
     2341 tgaagacata tttttcaaca gccggtaaaa gagtaccatt ttacccgaaa accccctgcc
     2401 tgtacccgaa acggc
//
LOCUS       DAXIHS010000148         2291 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXIHS010000148 DAXIHS010000000
VERSION     DAXIHS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.148"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            291..2093
                     /locus_tag="ACFOF2_002011"
     CDS             291..2093
                     /locus_tag="ACFOF2_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC7108253.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDEEDDEELEPVVRTAPVLSFHSDKEGTGEKEEVGASSNLTVYFDKKGVLK
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     KTEVKIGAKTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETININAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQL
                     KGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIG
                     YSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          718 a          570 c          557 g          446 t
ORIGIN      
        1 gcgggaatcc agcaaccgaa aaaccacagg aatctatcag caaaaacaga aaccccaccg
       61 ccgtcattcc cgcaaaagcg ggaatccaga cccgtcggca cggaaactta ccgaataaaa
      121 cagtttcctt agattccacg tcccagattc ccgccttcgc gggaatgacg agattttaag
      181 ttgggggaat ttatcagaaa acccccaacc ccaaaaaacc gggcggatgc cgcaccatcc
      241 gcccccaaac cccgatttaa ccattcaaac aaaccaaaag aaaaaacaaa atgaacaaaa
      301 tataccgcat catttggaat agtgccctca atgcctgggt cgtcgtatcc gagctcacac
      361 gcaaccacac caaacgcgcc tccgcaaccg tggcgaccgc cgtattggcg acactgctgt
      421 ccgcaacggt tcaggcgaat gctaccgatg aagaagacga tgaagagtta gaacccgtag
      481 tacgcactgc tcccgtgttg agcttccatt ccgataaaga aggcacggga gaaaaagaag
      541 aagtaggagc aagttcaaat ttgacagtat atttcgacaa gaaaggagta ctaaaagccg
      601 gaacaatcac cctcaaagca ggcgacaacc tgaaaatcaa acaaaacacc aatgaaaaca
      661 ccaatgaaaa caccaatgcc agtagcttca cctactcgct gaaaaaagac ctcacagatc
      721 tgaccagtgt tggaactgaa aaattatcgt ttagcgcaaa cggcaataaa gtcaacatca
      781 caagcgacac caaaggcttg aattttgcga aagaaacggc tgggacgaac ggcgacacca
      841 cggttcatct gaacggtatt ggttcgactt tgaccgatac gctgctgaat accggagcga
      901 ccacaaacgt aaccaacgac aacgttaccg atgacgagaa aaaacgtgcg gcaagcgtta
      961 aagacgtatt aaacgcaggc tggaacatta aaggcgttaa acccggtaca acagcttccg
     1021 ataacgttga tttcgtccgc acttacgaca cagtcgagtt cttgagcgca gatacgaaaa
     1081 caacgactgt taatgtggaa agcaaagaca acggcaagaa aaccgaagtt aaaatcggtg
     1141 cgaagacttc tgttattaaa gaaaaagacg gtaagttggt tactggtaaa gacaaaggcg
     1201 agaatggttc ttctacagac gaaggcgaag gcttagtgac tgcaaaagaa gtgattgatg
     1261 cagtaaacaa ggctggttgg agaatgaaaa caacaaccgc taatggtcaa acaggtcaag
     1321 ctgacaagtt tgaaaccgtt acatcaggca caaaagtaac ctttgctagt ggtaatggta
     1381 caactgcgac tgtaagtaaa gatgatcaag gcaacatcac tgttaagtat gatgtaaatg
     1441 tcggcgatgc cctaaacgtc aatcagctgc aaaacagcgg ttggaatttg gattccaaag
     1501 cggttgcagg ttcttcgggc aaagtcatca gcggcaatgt ttcgccgagc aagggaaaga
     1561 tggatgaaac catcaacatt aatgccggca acaacattga gattacccgc aacggtaaaa
     1621 atatcgacat cgccacttcg atgaccccgc agttttccag cgtttcgctc ggtgcggggg
     1681 cggatgcgcc cactttaagc gtggatgacg agggcgcgtt gaatgtcggc agcaaggatg
     1741 ccaacaaacc cgtccgcatt accaatgtcg ccccgggcgt taaagagggg gatgttacaa
     1801 acgtcgcaca acttaaaggt gtggcgcaaa acttgaacaa ccgcatcgac aatgtggacg
     1861 gcaacgcgcg tgcgggcatc gcccaagcga ttgcaaccgc aggtttggct caggcgtatt
     1921 tgcccggcaa gagtatgatg gcgatcggcg gcggtactta tcgcggcgaa gccggttacg
     1981 ccatcggcta ctcgagcatt tctgacactg ggaattgggt tatcaagggc acggcttccg
     2041 gcaattcgcg cggtcatttc ggtacttccg catctgtcgg ttatcagtgg taagggcttt
     2101 ttcgcctgtc tgctgttggg gcaggcggaa ggtttgaagg gaagggtggc gatttgccgc
     2161 ctgagacctt tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggggat
     2221 tttccatgag caccttcttc caacaaaccg cccaagccat gattgccaaa cacatcgacc
     2281 gtttcccgct a
//
LOCUS       DAXIHS010000149         2290 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXIHS010000149 DAXIHS010000000
VERSION     DAXIHS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.149"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFOF2_002012"
     CDS             complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFOF2_002012"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC7108254.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1600..2166
                     /locus_tag="ACFOF2_002013"
     CDS             1600..2166
                     /locus_tag="ACFOF2_002013"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC7108255.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          519 a          555 c          666 g          550 t
ORIGIN      
        1 atttaatcca ctatattttc ctgtttcagg ctcggcgttc tttgcacatc agtttcgcat
       61 ccagccagcc atcgccgccg gagatgatgt tgttggcaac gaagttgtcc aagtcgggca
      121 gcagggaaac ggcgcggccg ctgttgatga tgacgaccac cgccatcggt ttgtcgccca
      181 gccaataacc tgcaagggcg cggacattgt tgagcgtgcc ggtttttaag cgcaacagcc
      241 cgccgctttg tttgaagcgg ttgcgtaaag tcccgtctgt gccggcgatg ggtagcgtat
      301 cgatgaaatc ttgtgcaaac gggctgaaat aagccgtttc caacatttgc gccatcattc
      361 tcgccgttac cctttctttt ctggacaggc ctgaaccgtt ttccaaaacc aaatccgcaa
      421 tatcgatgcc cgacacggca agttcgcgcc ggacggcaga tgccgcctgt tcggaaacgg
      481 cgggcagttt gccgtcgccg ccgagtttga ggaagacgga acgcgcaatt agattgtccg
      541 aacgcttgtt catgtccgtc aaaatttcct tcatcggctt tgagtgtgca acggcaagcg
      601 tctgcgcgcc ttccggcgtg tcggatatgc cgatgccgtc tgaaatccgt ccgccgccga
      661 gcagccagtg gttggtaaaa ctttgccgga tcagttcgtc aagcgcgaac atccggacac
      721 cgacaggctt gcccaaacag ctctcgggaa tattgccgcg caatttcagc gtattgtccg
      781 aaaaagatgc acgcatcagt tttttgatcg aagggcaggc agcttgggag gcggtaattt
      841 tcaagttgtt ttgggcgaaa atatgcggca aaggcggatc ggtgaggatg tcggtactgt
      901 cggcggcatt gcgttcggcg cgcaccataa ccataccggc agacagcata gttggattgg
      961 ggggcgtcat aaacggcgaa ccgctgtcgg cttcgaaatc gtcggggctg ccgacttcgc
     1021 cccacaggct gtggtcgagc atcaggtgtc ccgtgatatt gcgtatgcct tgttcgcgca
     1081 actggcgttg gacggcaagc aggttttcct gattgaaaac ggggtcgccg ctgcccgccc
     1141 aatacaggtt tccgtcaagc gtgccgtcgt ttaccgtacc gttgctttta aactcggtcg
     1201 cccagcggta attgctgccg aaggttttga aggcggcaaa cgcggtaacg agtttcattg
     1261 tggaggcggg gttgacgggg acatccgagc ggtggtcaat gatgactttt ccgctgtcaa
     1321 gctcttggac atatacggcg atttcgtttt gcggaatgcg gccggtatcg agcgcgtgtg
     1381 cggcgaggga ggcgagaagc agcagcaggg aggccgctgt tttggggaaa ttcataggta
     1441 aatctgttac ataaaaaaag caattataag gcaaagccgg ataagtggga acgctgtggc
     1501 ggcgggccgg cttgtttgca ggggaaatca catatataat aatcgctatc attatgaaat
     1561 gattgagacg cacaaactta atattcagga ggaatgatta tggctaagaa aatcagtatt
     1621 ttggtgggca gcctgcgccg tgcttctttt gcgcgcaaag tggcattgaa tgcggcggag
     1681 atgttccccg aaggctggca ggcggaaatc gtcgaaatcg gacatctgcc gctttacaat
     1741 ttcgattatg acgaccctgc ggtggaagat gtgccgctgc ccgaaagcta cacggctttc
     1801 cgcgaaacga ttaaggcttc ggacggcatt ttgttcgtta cgtccgaaaa taaccgcacc
     1861 attcccgcct gtttgaaaaa tgcggtggac atcggctcga aaccgaatgc cgacgtggcg
     1921 tggaaaaaca aaccggccgg tatcatcagc cattccgtcg gcaagatggg cggttacagt
     1981 tcgcaaaaaa acctgcgcct tgcgctgtct tatttcgata tgcccgtaac cggacagccg
     2041 gaagtgtttt tgggcaattc gccgacgctg tttgatgaaa acggcaaatt gatcgactcg
     2101 gcaagggatt ttgttcagtc atatattaat cagtttgttg gtttgattga aagaaatgcc
     2161 aaataaacaa accaagtcga aaggccgcaa aaccggttcg ggttttgcgg cttttttata
     2221 tgggctgggc agcgtcaagg tttgccgttt gccggataat gcgcggcaaa tgaccggcgt
     2281 gtttatagcg
//
LOCUS       DAXIHS010000150         2274 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXIHS010000150 DAXIHS010000000
VERSION     DAXIHS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.150"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..848)
                     /gene="mafB"
                     /locus_tag="ACFOF2_002014"
     CDS             complement(<1..848)
                     /gene="mafB"
                     /locus_tag="ACFOF2_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC7108256.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAA"
     gene            complement(888..1013)
                     /locus_tag="ACFOF2_002015"
                     /pseudo
     CDS             complement(888..1013)
                     /locus_tag="ACFOF2_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(1342..1752)
                     /locus_tag="ACFOF2_002016"
     CDS             complement(1342..1752)
                     /locus_tag="ACFOF2_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108257.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1756..>2274)
                     /locus_tag="ACFOF2_002017"
     CDS             complement(1756..>2274)
                     /locus_tag="ACFOF2_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC7108258.1"
                     /translation="KWDWVKNTGYKTPAARPMQTLDGEMAGGNKPAKQATPNGKAGVS
                     TQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHKPDASSLDKIDSKSLFK
                     SDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDVVGYTDGKPTHIINIYRRKPNKDG
                     FRPVHGTPGKER"
BASE COUNT          607 a          486 c          436 g          745 t
ORIGIN      
        1 gccgcaagtt gtgcttccgg acttaaattt cctaaattgt gaataccctg taaagttttc
       61 cgtgctgccg cataggttac cgggtttacc gcactgtctg taattgcccc aactcccaat
      121 ccctgaaaac ccgttagaaa aggattggct gcaccttgga tgatgccgcg aatatcatcc
      181 atacggttac cccaccaatt tttattgggg tcgctttccc atatcagttt tcctgcttca
      241 tccgcacggc tgaaaaaacc agaggcggca ccggcatttt cttttagcca acggtctgaa
      301 aatggggctt gagggacaat attagtcttt gtttttgttg aagttccttt gacatagtag
      361 ctgtatatat cccttgctcc tccggggggc ggataatcgc tgccttgcgg tccgtcatat
      421 ccatcctccg gatggatttc cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg
      481 tctacaccgc cgctgaaatc agaagtgctt tttgaatcat gatgatcgaa cggactgtgt
      541 acttcatgtc cgtgccctga aaaatgggtt tcatagccga taacaccttc aaaccctgtc
      601 cgttcgtgtg taataggcag tatggggccg accgcagttg catcaaatgt ttggacggcg
      661 taaacccgat ttttaacact gccgcgacca tcaccgaaca gatggtattt gccacccggt
      721 tcgtagtgtt ttgcctgcat atcatcgcgc aaacgggcat ccaaaccgtt cgcatgacta
      781 atggggattt gcagcaacga tattgctgct gcaaacagca tcatgaattt ttgaataggc
      841 aaattcattt tgttttcctt ggtagcaagc ggttatggta gtgtgaatca aggttgccct
      901 tgtctatgtt gtcgcactac ttcatcgctg tatccatacc cctcatgact gttatcagtc
      961 tctacggatg gggcggagtt acccgtatga ttgccgtatg gtcggatact tgtatccgac
     1021 aaaaatattt gacgcgtcta tcgtttccga aatgccgctg ttgaaaaacg tcggattcga
     1081 gaatccgacc tacggttgct gaacaatgtt ttgtagaact tgctcaaaat ccatgtttcg
     1141 ccattttcct ttatatatac ctaatgcagc acgggttacg atttcgttta agtcataaat
     1201 agccatttac gtcctctgct aattttcagc gagtagattg ggttaccaaa cccaacaaaa
     1261 aagcagcctg aaaaatttca ggatgcttgt tttatgtttt ttatcaatga attaggtttt
     1321 aatccagcct gcgcttgcta gttaaacagt atttacataa aaatcttgca ctgaacaaac
     1381 aagcgtatca tgttcatcat gaaatgatgg gcataacgtt aatctacaaa tgtttacatt
     1441 taaagatatt ttttcagttt caatattttt aatttgtaag gttaatacat gaggatattt
     1501 gtgatatagc tgatttttta attgttcgtt gtctaatata gcaaaaggta attttgcaca
     1561 caaataacca ataactttgt ttttatgaaa aaaaactatc caattttcaa ttccttcatc
     1621 ccaatcaaaa gtaaaatccg ttccttttat gataaattta gcacaagata ttaaatctaa
     1681 cttaacccaa gtggatttat acagactcaa tgctgatgaa gttataatat tatctaaatt
     1741 ataaattttc ataaatcaac gttccttacc tggtgttcca tgcacaggtc taaacccatc
     1801 tttattcggc tttctacgat aaatgttaat tatatgtgtc ggttttccgt ctgtatagcc
     1861 aactacgtcg ttcgttggaa cttttgcctt attttgacta tcccataaat tatgtttatc
     1921 tgcatatcta gcagcatcta atgtagcttt ttccgcatca acatctgatt taaataaact
     1981 tttgctatct atcttgtcta gactagatgc atcaggtttg tgtcgtacca tactgagagg
     2041 gtcagtaata tatttaccat ctgtaatagc tgatccacca ttataggtag tatgcgtata
     2101 atttttttgt ggaacgagac tattttgcaa agattgttgt gtagaaacgc cagctttgcc
     2161 attaggagta gcttgcttcg ctggcttatt cccaccggcc atttccccat ctaaagtctg
     2221 cataggtcgg gcagcaggtg ttttatagcc ggtattttta acccaatccc attt
//
LOCUS       DAXIHS010000151         2112 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXIHS010000151 DAXIHS010000000
VERSION     DAXIHS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.151"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(175..>2112)
                     /locus_tag="ACFOF2_002018"
     CDS             complement(175..>2112)
                     /locus_tag="ACFOF2_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC7108259.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
BASE COUNT          394 a          464 c          707 g          547 t
ORIGIN      
        1 atcaacaacc gaaaatgaag aaaaacggtg ggctgaagcc caccctacgc aattgctttt
       61 gcggagatga cggggtttta gggtgaagat ggtgggagtt ggtgggagtt ggtgggctga
      121 agcccaccct acgcaactat cctacgcaac tactacgcaa ctactacgca actactacgc
      181 aattgccgcc gccttgcggt acagctcgaa caggaaggcg gtgcgttcgg cttcgtcgtc
      241 ggtgtttttg cctgttttgt agccgtaggc tttatcgact gccttgtcga gggcggcgtg
      301 ggctttgtct agggcggtat agcctgcgtc gggcgcatag agttcggcga gggtcggctc
      361 gggcaaaccg gcttcccgcg cttctcggcg gtattgtccg cgcgcgtcga ggacggcttg
      421 ggcggcggtt tcgacggcgg cgcggagcgg gtcttggcgg tcgttttcag acggcatccg
      481 gcagctttcg gggaagggga agttgttgta cacaacggta ttggaatagc ggtaacgact
      541 ttccagacgg ccggcaacgg tacgcataaa ggcgttgtgc atggtggagc tgaggatgcc
      601 gaagtggtag aggctggcgt ttggaagggt aaataccaaa tcgctgacaa ctgtttcata
      661 tgatagataa ccgatgggga cgaaacggcg gctttcggat gagacgcgtg ggataatcag
      721 aaaattgccg tctgaaggct ggcggatttt ttgaaaaagc cacggggttg ctgcatcttt
      781 ttgagtttgt ttgtcgctgc tggcttcgcg catggttttg actgcctgaa tacgggcttg
      841 aacttggggc atctgtttca ggtcgtggtt gcgtttaacg tcggatacgc cgtgaaacca
      901 caggcaccaa cgggttttgc cgttgataaa ttcctctgcg ccgacaaagg gacggatgta
      961 ttgttccgcc aagggttcgg cggcaatcag ggcttctttt tcatcggttg aaaggataag
     1021 gttgccgcct tcggtcggtt tgcttccgtt gaccatgtca ggttcgcaat gtacgggacg
     1081 gctgcgcttg gcaataatca catcgggcgc gtcaatcaga tacggattga tgttggccgc
     1141 ggcgtgtttt acgggttcgc ctttgatgtc gggatagtcg tagagggttt tttcagacgg
     1201 catttgcggc ttttggcgga agccgacgat gatgcagtgg acggcggctt tgcctgcggc
     1261 ttggctcgtc cattgaaagg tgcggtgggc aaagtggatt tcgatgtctt gcttcaaaag
     1321 gctgccccaa aggatttcga cctgctggcc ttggcagatg gaattggtgg aaacaaatgc
     1381 cgtctgaact tggggatgct gcgccatgat accggcggct ttgacatacc agttgcagac
     1441 gtaatccaac aggccgtagc ctttgatatg gccgcagatg ctttcgaggt cgtttttctg
     1501 ctctttggtt tggtaggtcg agccgataaa gggcggattg ccgaagatgt agtccgccgc
     1561 ctgccaaggt gtgcggaggc tgttggcgtg gatgatttcg gcggcgtctt cgagcggcag
     1621 cgtatggcag gcgacttcgc cgtcgaagcg gatttgcgtg cggatgttgc attggtggtt
     1681 tttgagccac atggcggtgc gggcgatgag gacggcaaat tcgtctattt cgatgccgtg
     1741 aaactgtttc agacggcatt ggacggacgg gctgtcgaat aagccgcctg ttttgtcttt
     1801 gagcgcttcg gcgatgatgt cgtcttcgag ggcgcggata cggtcgtagg cgacgataag
     1861 gaagttgccg cagccgcagg ccgggtcgag gaattgcagg ttttggattt tttggtagaa
     1921 ggcggcgagt tttttgtctt tgtcgcgttt gagggctttg acggcttcaa attcggcgcg
     1981 caggttttct aaaaaaaggc cgttgatgac tttgtcgatg ttggcggttt cggtgtagtg
     2041 tgcgcccgct tcgcgccgct cttgtgcgtc catgactgat tggaagagcg tgccgaagat
     2101 ttcgggactg at
//
LOCUS       DAXIHS010000152         1975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXIHS010000152 DAXIHS010000000
VERSION     DAXIHS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.152"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(195..1355)
                     /locus_tag="ACFOF2_002019"
     CDS             complement(195..1355)
                     /locus_tag="ACFOF2_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC7108260.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQW
                     GNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQPPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          515 a          498 c          464 g          498 t
ORIGIN      
        1 tatatgtgca aaccaagccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtcgc ggcaatttcg gtcgtactgt ttttggtttt gtcggcattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttc atcactcccg
      601 ctgccgagca agaacaactc aaaagcatca cggcccacat tggcgtgttt cgcgtattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gaccccggct tgccgacaac agccggaacg agagtaggcg gctgattttg cttattctga
      781 acaaaatgag ccggcgtata ggcggacttg ctgttttggg ccggaacgaa ttggacgctg
      841 ccgctaaaac cggaaaattc gggggaatcg tagcgtacgg aaaccgacat atcgtcgtgg
      901 cgtttgaaaa tacccaattg cgaagccaca tcattattgc tgtcccaagg atcaatggct
      961 tggctggcat cgtcaaactg atttgcaacg cgaccggcgc gcagcgtgcc gaattcgcct
     1021 gccaagccga taaaggattc cctgttgccc cactgggacg cgccgccgcc ggcaacggat
     1081 acgtcttgct caagctgcca aacagccttc agcccttcgc ccaaatcctc gctcccctta
     1141 aagccgataa acgagccgaa atcgctgatt ttcgtcctga tgcggctttt gcgcttagta
     1201 acctgaggtt gttgaatatt ttggagcggt tcggtcaact gcagctggat gttcctgcct
     1261 tccacgccgg ctttgatttc gccgtacagg ctaacatcgg caacggccgc aagcggcagt
     1321 gcggacaata cgagcgcggt aagttttttt cgcatatcgg cttccttttg taaatttgat
     1381 aaaaacctaa aaacatcggg caaacacccg atatgtcatc aattataccc cccccccccg
     1441 caaaaaacca tttttcagaa caaatatctg ataaatgccg caacctttat tttaaaaatg
     1501 attatatttt gatataaaac aatagcttat ttttcaaaaa cgttgtgttt ctacaacaca
     1561 attcaagcgc agacctcgtg cgggccgatg cgctgctgcc cggatgcagt ctcggctttt
     1621 taaaacgcca taaaaaaaca cacgcggcac tttatagtgg attaacaaaa acaagtacgg
     1681 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
     1741 cggttccgta ctatttgtac tgtctgcggc ttcgtcgtct tgtcctgatt tttgttaatc
     1801 cgctataaag accgtcgggc atctgcagcc gtcattcccg cgtaggcggg aatctggaat
     1861 ttcaatgcct caagaattta tcggaaaaaa ccaaaaccct tccgccgtca ttcccacgaa
     1921 agtgggaatc tagaaatgaa aagcaacagg aatttatcgg aaatgaccga aactg
//
LOCUS       DAXIHS010000153         1878 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXIHS010000153 DAXIHS010000000
VERSION     DAXIHS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.153"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            271..1689
                     /locus_tag="ACFOF2_002020"
     CDS             271..1689
                     /locus_tag="ACFOF2_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC7108261.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKITGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIIAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT          420 a          591 c          446 g          421 t
ORIGIN      
        1 tcctttcccc gacacgcgcg cgcgacccct gccgcaccgt gccgcacttt cgcgcccgga
       61 cggcatcgtt ccgccatccg gttctctgtt tcaaataccc ctgtttcaga aagaaattcc
      121 agatgcttca acacacagga cgacatataa agcaccgccc tatgtgttgc cctgatttgg
      181 aaggggttgc gcctcccaaa taaagtctga tcctgccgcc ccgaaggaca gatgtccgag
      241 tggcgaagtt tcaaccgaaa aggaaatacg atgaatattc acaccctgct ctccaaacaa
      301 tggacgctgc cgccattcct gccgaaacgg ctgctgctgt ccctgctgat actgctggcc
      361 cccaatgcga tgttttgggt tttggcactg ctgaccgcca ctgcccgccc aattgtcaat
      421 ttggactacc ttcccgccgc gctgctgatc gccctgcctt ggcgtttcgt caaaattacc
      481 ggcgtattgg cgttttggct ggcggttttg tttgacgggc tgatgatggt gatccagctt
      541 ttccctttta tggacctcat cggcgccatc aacctcgtcc ccttcatcct gaccgccccc
      601 gccccttatc agataatgac cgggctgttg ctgctgtata tgctggcgat gccgtttgtg
      661 ttgcagaaag ccgccgccaa aaccgacttc cggcacattg ccgtctgcgc cgccgttgtg
      721 gcggcagccg gctatttcac cggccatttg agttactacg accggggtcg gatggccaat
      781 atcttcggcg caaacaactt ctactacgcc aaaagtcagg cgatgctcta caccgtcagc
      841 cagaatgccg actttattac cgccggcctg gtcgatcccg tcttcctccc cttgggcaat
      901 cagcagcgtg ccgccacgca tctgaacgag ccgaaatctc aaaaaatcct ctttatcatc
      961 gccgaatctt gggggctgcc ggccaatccc gaacttcaaa acgccacttt tgccaaactg
     1021 ctggcgcaaa aagaccgttt ttcggtttgg gaaagcggca gttttccctt tatcggcgcg
     1081 acggtcgaag gcgaaatgcg cgaactgtgt gcctacggcg gtttgcgcgg gttcgcactg
     1141 cgccgcgcgc ccgacgaaaa atttgcccgc tgcctcccca accgtttgaa acaagaaggt
     1201 tacgccacct ttgcgatgca cggcgcgggc agttcgcttt acgaccgctt cagctggtat
     1261 ccgagggcgg gctttcaaga aatcaaaacc gccgaaaacc tgatcggtaa aaaaacctgc
     1321 gccattttcg gcggcgtgtg cgacagcgag ctgttcggcg aagtgtcggc atttttcaaa
     1381 aaacacgaca agggactgtt ttactggatg acgctgacca gccacgccga ctatcccgaa
     1441 tccgacattt tcaaccacag gctcaaatgc accgaatacg gcctgcctgc cgaaaccgac
     1501 ctctgccgca acttcagcct gcacacccaa ttcttcgacc aactggcgga tttaatccaa
     1561 cgccccgaaa tgaaaggcgt ggaagtcatc atcgtcggcg accatccgcc gcccgtcggc
     1621 aacctcaatg aaaccttccg ttatctcaaa caggggcacg tcgcctggct gcacttcaaa
     1681 atcaaataac aacaatgccg tctgaacgca ccaacagcct tcagacggca ttttgcagac
     1741 agaccgaccc cctcaagccc actttttcat catctccgat aaattgcttt gtatagtgga
     1801 ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt
     1861 gctgaagcac caagtgaa
//
LOCUS       DAXIHS010000154         1734 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXIHS010000154 DAXIHS010000000
VERSION     DAXIHS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.154"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(257..1246)
                     /gene="porB"
                     /locus_tag="ACFOF2_002021"
     CDS             complement(257..1246)
                     /gene="porB"
                     /locus_tag="ACFOF2_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC7108262.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQKGKGESKFVSTAGGVGLRHKF"
BASE COUNT          452 a          381 c          443 g          458 t
ORIGIN      
        1 cacaggcggg aatctagaca ttcaatgcta aggcaattta tcgggaatga ctgaaactca
       61 aaaaactaga ttcccacttt cgtgggaatg acggaatgta ggttcgtagg aatgacggca
      121 tattttgaca ttgaataaaa aagactaaaa acaggaaaaa gccaaacaga aaacagcgaa
      181 acagaaaaca ggaaaaagcc aaacagaaaa aagcctgtct ggcgacaggc tttttgttga
      241 taccaatctt tgcagattag aatttgtggc gcagaccgac accgccggca gtcgatacga
      301 atttgctttc gcctttgcct ttttgcaacc aaccggcaga aaccaaggca gaagtgcgtt
      361 tggagaagtc gtattccgca ccgacaacca cttggtcgta atcgttggtg tagtctgcat
      421 catcaaacga gcctttgaag ccgtgggcgt aagaaacgcg gggcgttacg ttgccgaagc
      481 ggtatgccaa ggtagcggca acttcggttt gagagttgtg cgaattgtct ttaaccagtt
      541 tcgcgtcttg ttgctgtacg gctacggaag cgtacagggc atcattgtcg taaccgctga
      601 ccaaacggtg aatctggtat ttctcaatct tcacgttatc cacattctga tgtcttttat
      661 aggcaccgcc atattgcatg aagaagccgc cgtttttgta gttgaagccg gcgtggtaag
      721 attcgctgtt atgtctgcct gcattgtcgt taagcgcgta ttgtacgctg ccgctgaggc
      781 cggcaaattc gggagaatcg tagcgtacgg aaatgaggcg tgcttcgggt tcggcaattt
      841 tgtttacacc caaatagtcg cttttgctat cccaaggatt gatgtcgccg gtgtctttca
      901 ggacgctgtt caaacggccg acgcgcaatt taccgaagcc acctttcaaa ccgatgaagg
      961 attggcggtt gccccaaccg gagtcagtac cggcgataga tgccttttgc tcaacctgcc
     1021 aaatggcttt cagaccgtta ccgaggtctt cttggccttt gaagccgatt ttcgaaccca
     1081 aatcaacgat gccggtaccg gtttcaacgc tagccgcctg agctccattg tgagctacgg
     1141 agcgggaagt ttctacgccg gctttgatgg tgccgtacag ggtaacgtca gccattgctg
     1201 caacaggaag ggctgccaaa gtcagggcaa tcagggattt tttcattgct gtattccttt
     1261 tttggttaag aaatttaagc cggccgggct ttccaagccg cttagctttg catttaccgc
     1321 cgacgttttc ggcgattcca tgtctgaaac tatagatgtt cctgtctgaa aaaacaaact
     1381 tctgcctgtt tttgtgtgga attcgatgtg tattttgaag ggcggataga gatattatgg
     1441 gtattttttg ttttataaca tatggttatt taaatttttt aagatttgca tttttacaac
     1501 acttactcgg gagggtattg gagggcattg caaaccgggg gttataaaga cagcaaaaaa
     1561 cgcgcacgtg gtttctttcg gaacgggcgg atgcaaaacc cgcccctgcg gcaggagata
     1621 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa
     1681 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggc
//
LOCUS       DAXIHS010000155         1698 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXIHS010000155 DAXIHS010000000
VERSION     DAXIHS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.155"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(100..423)
                     /locus_tag="ACFOF2_002022"
     CDS             complement(100..423)
                     /locus_tag="ACFOF2_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC7108263.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IIPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(411..650)
                     /locus_tag="ACFOF2_002023"
     CDS             complement(411..650)
                     /locus_tag="ACFOF2_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC7108264.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(773..1570)
                     /gene="truA"
                     /locus_tag="ACFOF2_002024"
     CDS             complement(773..1570)
                     /gene="truA"
                     /locus_tag="ACFOF2_002024"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC7108265.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          365 a          401 c          503 g          429 t
ORIGIN      
        1 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttaaagtt
       61 aatccactat aatgaacaca atccattcag actattcaat caggcaaaca tctcctgcaa
      121 tactgcaaac agtttttcag ccgtactgtt gtctaaattt ccaagatgtt tgaccaatcc
      181 ggctttatcc acagccctaa tctgttcggg caaaagcaaa ccgtctttat cctgaaagcg
      241 gacattgacg cggaacgggg caggacggct tccgctcgtc atgggaatga tcagcacagt
      301 cttgagatag ttgtgtattt caggaggaga gactacgaca caaggacgtg tctttttgat
      361 ttcgcttcct acggtcgggt ctaaggagac cagatagatt ccgccgcgta ctaccatatc
      421 cattctttat ccgcttcgtt ttcaatttcg gaaaaaaaat gctcctgctc ggtttcgaca
      481 agcattgcgg cagcttctgc ccatcccctg cgaacggtag gacagcttaa aatgatattg
      541 cccttttcaa ctgtaacagc caagctgtct actgccccta tttgacccaa taatgatttg
      601 ggcagaatca cgccttgcga gtttcccatt ttgcgtatgt tgagaatcat atacgtaccc
      661 tatttaccag tttatgtaat aacaatgtta ttaccttgat gaggtagtgt caatattgaa
      721 aaagattgcc tgacagtttg tccgatttca aaatctccgc gacaagaatg ttttaaagcc
      781 attcggggat ttgggggcgg atgatgccgt atgcctcggg atagtcgacg ccggtcaggt
      841 aaagtccgtc gggcatgaag gtcggcgggg ctttgaggcg gctgcgttct tgaatcagtg
      901 cggcgaagcc ttcgacgctg agtctgccgc tgccgacata gacgagcgcg cccatgatgt
      961 tgcgtaccat gtggtgcaaa aaggcgttgc cgtgcaaatc gaggcggacg agtcctgcgc
     1021 tttgggtaag gtcggcgcgg tagatggttt tgacggggga ttttgcctgg cattcggcgg
     1081 cgcggaagct ggagaagtct tgttcgccga tcaataaggc ggcagcccgg cgcatcggcc
     1141 cgatgtcgag tttgaggtgt gtccagcctg ccctgttttt gagcaggggg gaacggacgg
     1201 gggcggattc gagcaggtag cggtaatgcc gtccgtatgc gtcgaagcgg gcgtgaaatt
     1261 cgggggcgac ctgtcgggcg tgcaaaacgg caatgccttc gggcaggtgg gcatttacgc
     1321 cgcgcaccca tgcctgttgg ggacgggcgg cagttgtgtc gaagtggacg acttgggcgg
     1381 tggcatgcac gccggtgtcg gtcctgccgg caacggtggt ggaaaccgct tcccctgcta
     1441 tttgggcgag cgcggtttcc aatgccgcct gaacggtcgg tacgccgtca gcctgtttct
     1501 gccagccgta aaagcggctg ccgtcatagg atagggttat tgcccagcgt tgtttttgtg
     1561 cggtatccat cggatttggg attcggataa atgttcagac tgcattgtat cgcagatttt
     1621 gcagggaaac ggcaaatgcc cggggcgcgc ggcgttgttt ggggagttgg ggggggtgga
     1681 tgcagttgct acgaatcg
//
LOCUS       DAXIHS010000156         1683 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXIHS010000156 DAXIHS010000000
VERSION     DAXIHS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.156"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..260
                     /locus_tag="ACFOF2_002025"
     CDS             <1..260
                     /locus_tag="ACFOF2_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108266.1"
                     /translation="CPPKRAPEYHAGTVSESNFLNVAEKWLGENYKSYPNGRYVSQDG
                     MRQVRYGHHETNSSTHHGHFESYDKPNGRVIENSVVTIIQD"
     gene            267..536
                     /locus_tag="ACFOF2_002026"
     CDS             267..536
                     /locus_tag="ACFOF2_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108267.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGVLPKPVALSIVQYEDD
                     QGFYLFYLDETGREQTDTYHDTLDSAFKQAEFEFGIRKEEWIQSS"
     gene            696..1241
                     /locus_tag="ACFOF2_002027"
     CDS             696..1241
                     /locus_tag="ACFOF2_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC7108268.1"
                     /translation="MIIVEHLEHYLGEIENGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPILRGEIIRLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGGHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFVW"
     gene            1344..1683
                     /locus_tag="ACFOF2_002028"
                     /pseudo
     CDS             1344..1683
                     /locus_tag="ACFOF2_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
BASE COUNT          562 a          300 c          334 g          487 t
ORIGIN      
        1 cttgtcctcc caaaagagct cccgaatacc atgcaggaac tgtatccgaa tctaattttt
       61 taaatgtagc tgagaaatgg ctaggagaaa actataagtc atatccaaat ggtcgatatg
      121 tatcacaaga tggaatgcga caggtacgat atggtcatca tgaaacaaat tcatctacac
      181 atcatggtca ttttgaatca tatgacaaac ccaatggacg tgtaatagaa aatagtgtag
      241 taacaattat tcaggattaa aaaattatga gtgtaaaaga attattcaaa gttatcttag
      301 ataaaaataa agattttcct attagaacga ttcatcgcac tccaatgggg gtacttccca
      361 agcctgttgc tttaagtatt gttcaatatg aagatgacca aggattttat ttattttatt
      421 tggatgagac cggtcgggaa caaacggata cttatcatga cacattagat tcggcattta
      481 aacaggctga atttgaattt ggaattagaa aagaagaatg gatacaaagt tcttaggtat
      541 caaacgtaag atgggttgaa aaaatcaaca atcaaaaggt cgtctgaaac catgtttggt
      601 ttttcagacg acttttccgt taataaaaaa tagcgatatt ggaacccaaa ccagcctacg
      661 gcttgttaga atagggatga aaagtttaag aaaaaatgat tatagttgaa catttagagc
      721 attatttggg cgaaatcgaa aacggtataa aatgcttgga tcgacgttat catctcagcg
      781 tttccgtatt cccatcacaa ccatataaag gggtaactac tttttctact ttaggtttga
      841 atcgatatga cttgaactat aagagtaggt ttgaattaat ttttacctgt tctgaagaat
      901 ggaataaaga aaatattgca gcttttctat caggagtagc tgaatatttg attgacaata
      961 gaaaacctat tttgagaggt gaaattattc gattacctag ggtaattatc gaaggtagca
     1021 aaatggacgc tctgtatgtt tccgccccat tttatttcga tgatgatttt caggtctgtt
     1081 atggcggaca ctacaatact gtttttcctt tgcttgtccc gttgtataaa caggaagcag
     1141 aattggtgga aaaaaagggt tggaatgctt ttgagcagtt cttgctggat aatgaagttg
     1201 acaacctttc ggatatgaat aggaaaccgt ttgtttggta aaggattttc ttaaatttga
     1261 ttaaaggtcg tctgaaatta agcttgtttt tcagacgacc tttccttcat ttgaaacaga
     1321 atattgagaa ctgagttctt caaaaatcct acacctgctc cttccacggc agcaccttgg
     1381 tcaaaacggc agacggctac aaagccattg cccatattca agccggcgac cgcgtcttcg
     1441 ccaaagatga aaccagtgga aaaactggat acaaacctgt taccgcccga tacggcaatc
     1501 cgtaccagga aaccgtttac attgaaattt cagacggcat cggtaacagc caaaccctaa
     1561 tttccaaccg catccatccg ttttacagtc aaggaaaatg gatacaggca ggtcgtctga
     1621 aaaaaggcga caccctgctt tccgaaagcg gtgcaaaaca gacagttcaa aacattaccc
     1681 tca
//
LOCUS       DAXIHS010000157         1630 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXIHS010000157 DAXIHS010000000
VERSION     DAXIHS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.157"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            208..1407
                     /locus_tag="ACFOF2_002029"
     CDS             208..1407
                     /locus_tag="ACFOF2_002029"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC7108269.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
BASE COUNT          406 a          481 c          395 g          348 t
ORIGIN      
        1 attggtaggg gaaaacaggt ttcgtgagtt ctacattctg gattcccgcc acagcctgtc
       61 ctcgcgtagg cggggacgga ataacgatag aaaatgcggc atacgctttg cccaaagagg
      121 ccgtctgaaa caccttgcgc ctgatgtctg cctttttcag acgaccccac accaaaaaaa
      181 caaccacaaa ctacaaggag aaacatcatg tccgaccaac tcatcctcgt tctgaactgc
      241 ggcagttcat cgctcaaagg cgccgttatc gaccgcaaaa gcggcagcat cgtcctaagc
      301 tgcctcggcg aacgcctgac cacgcccgaa gccgtcatta cgttcagcaa agacggcaac
      361 aaacgccaag ttcccctgag cggccgaaat tgccacgccg gcgcggtggg tatgcttttg
      421 aacgaactgg aaaaacacgg tctgcacgac cgcatcaaag ccatcggcca ccgcatcgcc
      481 cacggcggcg aaaaatacag cgagtctgtt ttgatcgacc aggccgtaat ggacgaactc
      541 aatgcctgca ttccgcttgc gccgctgcac aaccccgcca acatcagcgg catcctcgcc
      601 gcgcaggaac atttccccgg tctgcccaat gtcggcgtga tggatacttc gttccaccaa
      661 accatgccgg agcgtgccta cacttatgcc gtgccgcgcg agttgcgtaa aaaatacgct
      721 ttccgccgct acggtttcca cggcaccagt atgcgttacg ttgcccctga agccgcacgc
      781 atcttgggca aacctctgga agacatccgc atgattattg cccacttagg caacggcgca
      841 tccattaccg ccatcaaaaa cggcaaatcc gtcgatacca gtatgggttt cacgccgatc
      901 gaaggtttgg taatgggtac acgttgcggc gacatcgatc cgggcgtata cagctatctg
      961 acttcccacg ccgggatgga tgttgcccaa gtggatgaaa tgctgaacaa aaaatcaggt
     1021 ttgctcggta tttccgaact ttccaacgac tgccgcaccc tcgaaatcgc cgccgacgaa
     1081 ggccacgaag gcgcgcgcct cgccctcgaa gtcatgacct accgcctcgc caaatacatc
     1141 gcttcgatgg ctgtggcctg cggcggcgtt gacgcactcg tgttcaccgg cggtattggc
     1201 gaaaactcac gtaatatccg agctaaaacc gtttcctatc ttgatttctt gggtctgcac
     1261 atcgacacta aagccaatat ggacaaacgc tacggcaatt cgggtattat cagcccgacc
     1321 gattcttctc cggctgtttt ggttgtcccg accaatgaag aactgatgat tgcctgcgac
     1381 actgccgaac ttgtcggcat cttgtagcca aaaaagggac gagtccgcac aatgccgtct
     1441 gaaaccccaa acgcccgatt aggctgatga ggattttaga cggcattgtt catttttttg
     1501 ttatcttgca tttttgtgcg gacggtggaa tttcatcctg taaacataaa tatttgtcgg
     1561 aaaacagaaa ccctccgccg ccatttctac gaaagcagga aaccagcaac gcaaagcgac
     1621 agggatttgt
//
LOCUS       DAXIHS010000158         1576 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXIHS010000158 DAXIHS010000000
VERSION     DAXIHS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.158"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            252..500
                     /locus_tag="ACFOF2_002030"
     CDS             252..500
                     /locus_tag="ACFOF2_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108270.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
     gene            1376..>1576
                     /locus_tag="ACFOF2_002031"
     CDS             1376..>1576
                     /locus_tag="ACFOF2_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108271.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIY"
BASE COUNT          502 a          208 c          338 g          528 t
ORIGIN      
        1 tttattgtct ttaaatgaga taacagataa aaccttattt aaagaaaatg tagtttatcg
       61 tttcagtaag tattaggtgt gatttttatt aagttatttg aggttttcgg taagtgtaga
      121 tataaggttg agttataacc aatcattcag gctctgttag gcttggcaac tcaacctgtg
      181 cttgctgaaa ttgggtatac agttatacgt tttaagcggg cttcgctccc tttatcattt
      241 aagggtaatt tatgaatatt aaaaatgaaa tagttgcttt aatttctaaa aaaattgatg
      301 gggggcaata tagttcttta gaaaattttg ctttatccgt actaagggaa aaaattataa
      361 aaaatactgt ttccagtgat gaaaaattat tgattattaa tgatttttta tcaggttttc
      421 ttgagtttga tcctgagacc gggaaacctg tcggggagac gcttaaaatt gagcaaatga
      481 ttgatttctt tttagtctaa atcatacata ccagtgaaca agctaggctt atatccggct
      541 tttaaatacg attgtcaggt ttatacttgg gataaaatca aaaataaagt tgtttaaact
      601 ctagtagatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg
      661 gaaccgattc actcggtgct tcaacacctt agagaatcgt tctctttgag ctaaggcgaa
      721 gcaacgccgt actggttttt gttaattcac tatattgcat ggtgcagcat taaatcagtc
      781 ttctagcgcc gcaatgggaa aacatagaag ataaacgaaa tggatgatct aatactatat
      841 tttttatcag gtatattcgg aaatcaagtt gctgaatgta ttataaaaaa caaccgtgag
      901 ataaaagttc cctttattgt gctttacgca atatttttta cgttgattta tactgtggct
      961 ttgttgtttc tcagtctgat ctattgggtc aatggtgcgg agattgcatg gaaggggata
     1021 ggtattttca gtatgtcggt cagtttttgt atagtcttct gtctttattt gattgacaag
     1081 gcaggaagat gtaaggataa gaaacaatag ggatcagatg cggcagcaaa cgtagattgg
     1141 gtctaggctt aacctgcgtt tgctgcatct ttggaagaaa tagtggattc gaaaaaatag
     1201 gaagaattct gattaatggt atgcgaggtg tagcagtggg gactgttgct ggtggcataa
     1261 atggttatgc aggttctacg ggtaagaaca ctgacattcg ccgttaaata ataaataaac
     1321 caacttccaa ccaaacctgc tatcggtttg gttggaagtg agaggaggta aggtagtgtt
     1381 tgctgttatt ttcttttcga cattgggctg tattttagca tggataagag atattcctaa
     1441 aataaaatca aaaaaaattt tggcaagatc cttatatatt ataggaataa taaatgtaat
     1501 aatcagctat gtattaatta aaaatatatt ggtttctgtt tcggatggag gtggaataaa
     1561 atatgttgca atatat
//
LOCUS       DAXIHS010000159         1485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXIHS010000159 DAXIHS010000000
VERSION     DAXIHS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.159"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            407..>1485
                     /gene="fic"
                     /locus_tag="ACFOF2_002032"
     CDS             407..>1485
                     /gene="fic"
                     /locus_tag="ACFOF2_002032"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC7108272.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLR"
BASE COUNT          447 a          365 c          335 g          338 t
ORIGIN      
        1 gtaggaatga cggtttaaaa gttacccgaa acctcaaaaa aaaaggctgt gttttaatat
       61 agtgttgata gacgtacttg gcttccatgt attcaatcgt ggaaatctat atcttcgtcc
      121 tcgccgaaat agtctatgcc cgatatacaa ttttgataca caaacttgga aatatgggta
      181 tcgtcgccgg agcgatagaa tgcagacagt tatatatata cggttttttt tagggaagcg
      241 ggcagatgaa gggcggattt tgaattaacc ccatgcaaat tgacttttgc gggcggtttt
      301 accgcccata tatttacaaa agccaaattt ttaaacatat atccttgata tatacacggc
      361 gtaaatatat actggaaaca tctttaaatt ttccgaaatt ttaaacatga gcaactggaa
      421 acccgatatt ccctataacg atttaccacc cctgccgcca aaacaggata ttgaaagcaa
      481 aaccatcctg aaacgttgta tagccgcccg tgcatccctt gcccgtttaa agcaggcggc
      541 agaattgata ccgaatcaag ccatgctgat taacaccctt cctgttatgg aagcccgtgc
      601 aagttcggaa attgaaaaca tcgtaaccac cacggacaag ctgtttcaat ccctgcaaat
      661 ggatacggaa cggcaagacc ctgccacgaa agaagccctg caataccgca ccgccctgtt
      721 tgcaggctat gaatcactga cgagccgccc tttatgcaca caaaccgcca tcatggtctg
      781 caacgccatc aagcacccct acgaaatggc catccgcaaa acaggcggca cagccctaaa
      841 aggaggcaac agcggaaatg ttgtctatac cccgcccgaa ggagaagaaa ccatacgcgg
      901 caagctggca aattgggagc ggtttattca cgaaagcggc gatttagacc cgcttatcat
      961 catggcggcg gcacattacc aatttgaagc catccatccg tttacggacg gcaacgggcg
     1021 gacggggcgc atattgaaca gcctgctatt gattgaaaaa gggcttttgg atttgcctat
     1081 tttgtatttg agccgctaca tcatcgaaaa cagggcggac tattaccgcc tgcttttagg
     1141 cgtaaccgaa cggcaggact gggaaagctg gataatctac atcttagacg gcgtagctga
     1201 caccgccgat tggacggtat cgaaaataga tgcgatacgc cgcctgttcg agcagacacg
     1261 gcaacacata cggacacacg cacaaggaat ctacacgcac gaactggtaa atcttctgtt
     1321 tgagcagcca tatacacgca ttgccaacct agaagcggca gggatagcca aacggcagac
     1381 ggcctctaag tacctgaaag agctttcaga cataggtgtg ctgcaagaaa tcgccatcgg
     1441 cagggacaaa ctattcattc atccgcgcct aatggaacta ttgcg
//
LOCUS       DAXIHS010000160         1455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXIHS010000160 DAXIHS010000000
VERSION     DAXIHS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.160"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(101..346)
                     /locus_tag="ACFOF2_002033"
     CDS             complement(101..346)
                     /locus_tag="ACFOF2_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108273.1"
                     /translation="MLKSIELNSHVRNRLAAYLKGRGMDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            463..888
                     /locus_tag="ACFOF2_002034"
     CDS             463..888
                     /locus_tag="ACFOF2_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC7108274.1"
                     /translation="MYSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYVR
                     LSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGVL
                     LRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            900..1376
                     /locus_tag="ACFOF2_002035"
     CDS             900..1376
                     /locus_tag="ACFOF2_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC7108275.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
BASE COUNT          296 a          349 c          386 g          424 t
ORIGIN      
        1 accagacaag tcggattccc gcgcgggcgg gaataacgat aagcgtaatt tcaatctgcc
       61 atattgtctt ttcacttgcc tgaaaacaga tttcagccgc ctatccctgc atccggcggc
      121 tgatgtagtc ggcaatcaaa tcccgctttt cccaaaaaaa tttctgcatt ttttgttcgg
      181 aatacagttt gcggaccaga gtgggcagac cgctgtggac gatggcggcg atttctttgt
      241 tgccttcttc ttcctgcatt gccgtctgaa aatccatgcc cctgcctttc agatatgctg
      301 caaggcggtt gcggacatgc gaattgagtt cgatagattt gagcattttt cctccgtttt
      361 cgataacgat tatatttaat ccgtctgtat gccttatttt atcatgtttc acgatcggtt
      421 tgcctgtaca ataccgcttt tcagtatgat gtgctggcat ttatgtattc ttggttcaag
      481 ctgtttcact tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt
      541 ttcgtcaata tggcgatgat tgatgcgccg cgcggcaatc ccgagtatgt gcgcctgtcg
      601 gatatggcgg tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc
      661 ggcgcggcga taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg
      721 tgtttgggct tgatactctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt
      781 caggattaca gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg
      841 ctgctgatgg ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta
      901 tgccgataga aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg
      961 acgaaccgct gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg
     1021 tccgcattgc cggacaacgg gcgtggctga cgctgaaagg ctatatttcg gaaatcagcc
     1081 gcagtgagtt tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt
     1141 gcccgtttaa aatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa
     1201 tcgatgtttt ccttggcgaa aacgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg
     1261 agaatgccga ttttgaccgt ccggactggc tggggcgcga aatcactgcc gacggtatgt
     1321 ttaccaatgc ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaaacg
     1381 gcttcagacg gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat
     1441 ttctgctttc tttgt
//
LOCUS       DAXIHS010000161         1380 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXIHS010000161 DAXIHS010000000
VERSION     DAXIHS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.161"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(141..1310)
                     /gene="prpF"
                     /locus_tag="ACFOF2_002036"
     CDS             complement(141..1310)
                     /gene="prpF"
                     /locus_tag="ACFOF2_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC7108276.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
BASE COUNT          266 a          380 c          389 g          345 t
ORIGIN      
        1 tatcggggtt tccgtgttct tccttgtgat ttgccgcctt ccgccacagg gcaagggaaa
       61 ggaatggtca gtgatttgat gtttacttgt ttttcagacg acctgtttat ggctcgcggg
      121 caaacccatg ctacgtcaat ttaaaaacaa tcttccggca ccctgaccca accttccatc
      181 atcacgcgtg cgctgcggct cataaccgct ttggtggccg tccattgtcc gtcctgacat
      241 tcggcggctg caccgacgcg caatgtgccg gaaggatgcc cgaagcgcac ttctttacgc
      301 gttccgccgc ctgcggcaag gttgaccagc gtaccgggca cggcggcggc ggtcgcaatg
      361 gcaacagagg cggtacccat catcgcgtgg tgcaatttgc ccatgctcag ggcgcgtacc
      421 agcaaatcga tgtcggcggc attcacggtt ttgccactgg aggcggtgta atcggcggcg
      481 ggcgcgacga aggcgacttt cggcgtgtgc gcgcgggcgg cagcttcgga tacgtcgctg
      541 attagaccca ttttcagcgc accgtaagcg cggattttct cgaatttttc caaagctgcg
      601 gcatcgttgt tgatgtcgtc ttgcaactct ttgcccgtgt agcccaagtc ggcggcattc
      661 aggaaaacgg tcggaatgcc cgcgttgatg agcgtggctt tcaaacggcc tatattcggc
      721 acatcaattt catcgaccaa attgccggtt gggaacatac tgccttcgcc gtcggctgga
      781 tcaagaaatt cgatttgtac ttcggctgcc gggaacgtta cgccgtcgag ctcaaaatcg
      841 cctgtttcca aaactgcgcc gttttgcatc ggtacatggg caataatggt tttgccgatg
      901 tttttctgcc agattttgac tgtgcagatg ccgtctgaag gaatcttgcc tttatcgacc
      961 aagccttgct cgatggcaaa tgcgcccacg gcggcggtga ggttgccgca gttgccactc
     1021 caatcgacaa aaggtttgtc gatggaaact tgcccgaaaa ggtaatcgac atcgtgatcg
     1081 gcgcgttcgg acttgtccaa aatcaccgcc ttgctggtgg acgagctggc gttgcccaaa
     1141 ccgtctatct gcttgccgta gggatccggg ctgccgagta cgcgcaagag gattttgtcg
     1201 cgtgcgcttc ccgcttcccg cgccgcctcg ggcaggtcgg aacgtttgaa aaacacgcct
     1261 tttgatgtac cgccacggta gtaaacggcg ggaattttaa tttgcggcat ttctagattc
     1321 tccttatgta gcgtgggctc tgcccacgat ttttatagtg gattaggctg ccactccgac
//
LOCUS       DAXIHS010000162         1313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXIHS010000162 DAXIHS010000000
VERSION     DAXIHS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.162"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..292)
                     /locus_tag="ACFOF2_002037"
     CDS             complement(<1..292)
                     /locus_tag="ACFOF2_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC7108277.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELI"
     gene            577..1248
                     /locus_tag="ACFOF2_002038"
     CDS             577..1248
                     /locus_tag="ACFOF2_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC7108278.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          357 a          280 c          330 g          346 t
ORIGIN      
        1 tgatcagttc gtcgaaacgg caaaacaggt tgaaatcgat gcgggtgatg aggctgtgtt
       61 cgagttcggg atcggagagg ctgtgccatt gtccgagcag gacggctttg aacatggaca
      121 acagcgggta ggcgggacgg ccgcggtggt ctctaaggta acgggttctt tgacggttca
      181 ggtattgttc gatcggctgc caatcaatta cctgatccaa cttcaatagc gggaaacggt
      241 cgatgtgttt ggcaatcatg gcttgggcgg tttgctggaa gaaggtgctc atgagaaatc
      301 ccctaaatgt cttggtggga atttagggga ttttggggaa ttttgcaaag gtctcaaacg
      361 gatgaaatca accgatattc aaacacagtc atttttagcg cattttcagc gtatcgttaa
      421 tgcggaaaat ttcgtgaaca ggttttttgc acaggtctcc gcctcgtttc gcgggatggg
      481 aaaccgtatt agaaaacgga cgcacgcaac ataaaccccg aaagtgatga taagatgatg
      541 atttaacgta ctgctttaat tatttaagga attatcgtgt ttcccgacaa atacaagttg
      601 agtttggaag aaaatatttt tctggcaaag aaagtattgg ttgcccaaat tcacaacctc
      661 agccgttttg agaattgtca gacgaccttg ttgcagaccg aacaaattat ccatggcaaa
      721 aatgtagcct ccgcgtcact ggaagacatc caaaccatct tgaacctgaa acgtgcctat
      781 caatatgtga tttcgcatat ttcaaacggc gaaccggtcg atatttcact ccttaaaaaa
      841 atcaacaaca ttgttgccaa ggacgattct ttggcacccg gtgatttccg taccggttcg
      901 gtcggcgtaa cgctattgga cggttcccgt catgccccaa atccggtgaa ggaaattgaa
      961 gtggctcgcg tgttacaaaa tatcggactg caaagcggtt cgacgacgga ggcagccgtc
     1021 cgttttatgc tttattgtat gcggcagcag gttttttggg acggcaacaa acgaacggca
     1081 accttatttg ccaacggtct gatgatggcg gggggctgcg gcatcttgga aatctccgaa
     1141 atgcagatgc cgcaattcaa tgaaaaactg tccgcattct atcgctccgg cgacgatacc
     1201 gatatttcca agtttgtgta tcaaaattgt atatcgggca tagactgaga cctttgcaaa
     1261 attccccaaa atcccctaaa ttcccaccaa gacatttagg ggatttctca tga
//
LOCUS       DAXIHS010000163         1293 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXIHS010000163 DAXIHS010000000
VERSION     DAXIHS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.163"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(235..498)
                     /locus_tag="ACFOF2_002039"
     CDS             complement(235..498)
                     /locus_tag="ACFOF2_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108279.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(517..>1293)
                     /locus_tag="ACFOF2_002040"
     CDS             complement(517..>1293)
                     /locus_tag="ACFOF2_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC7108280.1"
                     /translation="EIVGEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTG
                     GNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDA
                     AAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTT
                     PAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLP
                     NDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          361 a          300 c          220 g          412 t
ORIGIN      
        1 aattaggcgg tatttggggc gaaaacagcc gaaaacctgt gttgggattt cggttgtcgt
       61 gagggaaagg aattttgcaa aggtctcaat tggtgttggt gaatgccttc ggctgccagc
      121 agttgcattg aggccgtctg aaaaacggat attcagacgg cctattgcta ctttaaataa
      181 tatttgggtt taaaatatca tcgtatttcc caaattcggg gaacagaaaa tcaattattt
      241 aaatttttca tcctcataat atgggtgatc aaaaagctct ccttcccaca tatttatttc
      301 ttctatgcct ttatctttca taaactcatg ataatcataa aaatcttttc catattgtcc
      361 gtaaaaaatt ttcatatccc caagccacat agcataacat tcatcgcata aaatgacctc
      421 cccagagaaa tatttaggct ttgcatgata aaggtagcct tgctcacatc ttgggcaaat
      481 tttaatttct ttatccataa gagattcctt tcttaattat ttctgaggat atgcagaaat
      541 gattttattt gttccttttt gcacaacaat ccgcactttt gtttctcctt ttgttccaat
      601 aggtttcttc atatctacaa gatatacgga tgaatcattt ggcaaaggat ttcctttcat
      661 tctccaagct tcatcaacca aaggcaaaac ttctttacgg gacacattaa aaacagaatg
      721 aacaggtttg tttggatttg gaattgtatg ggctaaaaca tgtttaatcc tattaccatg
      781 cttagcatct aacccataat ctaaccccgc cggtgttgtc caaaccttat taaccttatc
      841 ataagtaagc ttgattccgg tctgcttctg aatttcctgt atggctttag ccgtatcaac
      901 tgcttcgtct cccgccttca gaacataagc cccgcctcgg ctgattttag tcgcacctgc
      961 cggaacaaag ggaacggcag ctgcggcggc atccacacca aaatcaatcg ccgcttctcg
     1021 agccatctgc ttatcgccaa tagatttggc gtaaatccat ttaccgccgt cccatacgag
     1081 gttgccgata tcccaaataa tatctaatgc attattttta acagctgttt cggaagcatt
     1141 tgccgccaca tcaacattgc ctcctgtaac acctacagtc gctccggcta ccaattttgc
     1201 atacgcagta atattcgctt tagctttttc aatttcagaa gcagtcatac cgctgaaatc
     1261 ggtattctta accaaagcct ccccgacaat ctc
//
LOCUS       DAXIHS010000164         1288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXIHS010000164 DAXIHS010000000
VERSION     DAXIHS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.164"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            267..1190
                     /gene="lpxC"
                     /locus_tag="ACFOF2_002041"
     CDS             267..1190
                     /gene="lpxC"
                     /locus_tag="ACFOF2_002041"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC7108281.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          314 a          326 c          309 g          339 t
ORIGIN      
        1 ttttctgttt tggttttatg tccttgtggg aatgacggtt tacgcgaaaa tgccgtccga
       61 aaggttttcg gacggcattt tttgcgtttt tcaggagggg ggcggcttcg tcgccttgtc
      121 ttgatttttg ttaatccact atacattttt atgcggctta cgtattgaaa acggatacat
      181 tggcaaatac tatggaaaaa catagcttgt ttttccggtt ttatcgtatt attccaaaac
      241 ttaccccatt gataaggaag cggtttatgc tgcaaagaac tttggcgaaa tccatcagcg
      301 ttaccggagt cgggctgcat tcgggcgaac gcgtcgcgct gaccctgcac cccgcgcctg
      361 aaaacagcgg gatttccttc cgccgtaccg atttggacgg cgagatgggc gaacaaatca
      421 agctgacccc ttatttgatc aacgataccc gcctttcctc caccatcgtt accgacaaag
      481 gcgtgcgcgt cggcacgatc gaacacatta tgtccgcgct gtccgcctac ggtatcgaca
      541 acgcgctgat tgagctgaac gcgcccgaaa tcccgattat ggacggctcc agcctgccgt
      601 ttatttacct tttgcaagat gcgggcgtgg tcgatcaaaa ggcgcaaaag cgttttttga
      661 aaatcctcaa gcctgtcgaa atcaaagaag cgggcaaatg ggtgcgcttt acgccgtatg
      721 acggctttaa ggtaacgctg accatcgaat tcgaccatcc ggttttcaac cgcagctcgc
      781 ccacttttga aattgatttc gccggcaaat cctacatcga cgaaatcgcg cgcgcgcgca
      841 ctttcggctt tatgcacgaa gtggaaatga tgcgcgccca caatctgggt ttgggcggca
      901 atttgaacaa cgctatcgtg attgacgaca cggatgtcct gaatcctgaa ggcttgcgct
      961 atcccgatga gtttgtgcgc cacaaaatcc ttgatgccat cggtgatttg tatatcgtcg
     1021 gacatccgat tgtcggcgcg tttgaaggct acaaatcggg acacgccatc aacaacgcac
     1081 tcttgcgcgc ggttttggca gacgaaacgg cttacgaccg ggtggaattt gccgacagcg
     1141 atgatttgcc cgacgcattt cacgagctga acatcagaac ttgtggataa cgctttctat
     1201 tttttccggt aaattcctaa aacttaaaat ttcatcattc ctacaaggac aaaaaaccaa
     1261 aacagaaacc taaaattcgt cattcccg
//
LOCUS       DAXIHS010000165         1148 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXIHS010000165 DAXIHS010000000
VERSION     DAXIHS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.165"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            342..990
                     /locus_tag="ACFOF2_002042"
                     /pseudo
     CDS             342..990
                     /locus_tag="ACFOF2_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
BASE COUNT          302 a          322 c          237 g          287 t
ORIGIN      
        1 agctaaggcg aggcaacgct gtactggttt ttgttaatcc actatagtac ggaaccgatt
       61 cacttggtgc ttcagcacct tagagaatcg ttctcttttt tgttcatccg ctatattgtg
      121 ttgaaacatc gccccaaacc tgatatagtc cgctcctgca acatcattga aaatcgttct
      181 ttttaatcag ttaaaaccga atacggagtc gaaaatgaat ccagccccca aaaaaccttc
      241 tcttctcttc tcttctcttc tcttctcttc tcttctcttc tcttctcttc tcttccgcag
      301 cgcaggcggc aagtgaagac ggcagccgta gcccgtatta tgtgcaggcg gatttagcct
      361 acgccgccga acgtattacc cacgattatc cgaaaccaac cggtacgcac ggtaagcgat
      421 tatttcagaa acatccgtgc gcattccatc cacccccggg tgtcggtcgg ctacgatttc
      481 ggcggctgga ggatagcggc agattatgcc agttatagaa aatggaaaga aagtaattat
      541 tctaaaaaag ttactgaatt taaacaccaa aacggcacca aaaaagaaga caaaacagaa
      601 catcaaggaa acggcagctt ccacgccgct tcttctctcg gcttatcagc catttacgat
      661 ttccaaataa gcgataaatt caaaccctat atcggcgcgc gcgtcgccta cggacacgtt
      721 aggcatcaag ttaattcggt caacaaagaa actattactg ttactccaat gggtggcggt
      781 tctattatac aaggcccgac cagcaaacct gcttatcacg aaagccacag catccgccgc
      841 ttgggtcttg gtgtcatcgc cggtgtcggt ttcgacatca cgtccaatct gactttagat
      901 gctggctacc gttaccacaa ctggggacgc ttggaaaaca cccgcttcaa aacccacgaa
      961 gcctcattgg gcgtgcgcta ccgcttctga ttccccgatg ccgatgccgt ctgaaccttc
     1021 agacggcatt tttgatgcac ttgccgttta caggcgcggg gcgggcgtag tgaaataccc
     1081 aaaccgtcat ccccgacaac accgcaatct tgaaacccgt ccgacaacac cgcaatcttg
     1141 aaatccgt
//
LOCUS       DAXIHS010000166         1125 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXIHS010000166 DAXIHS010000000
VERSION     DAXIHS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.166"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..489)
                     /locus_tag="ACFOF2_002043"
     CDS             complement(<1..489)
                     /locus_tag="ACFOF2_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element IS1016C family transposase"
                     /protein_id="HFC7108282.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTNSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHI"
     gene            complement(609..>1125)
                     /locus_tag="ACFOF2_002044"
     CDS             complement(609..>1125)
                     /locus_tag="ACFOF2_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108283.1"
                     /translation="NQCCPRIKNKIGGSLQPHQMPSEHVFGRRFVGGYRSGTSEKGFQ
                     AVFGRVVTVENVIRLPEKFGRFSGSLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRK
                     WMFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQ
                     SSYSHKRFIVN"
BASE COUNT          285 a          295 c          225 g          320 t
ORIGIN      
        1 aatgtggttt tgacggtctg caaactcctt ggaatggttg atgcggtaat ggataaaacc
       61 gctcacgtcc aacttgtcgc agctgctcag actattggta taaacaatgc tgtccggcat
      121 gattttcttt ttgatgacag ggagtaacgt ttcagacttg gcattatcca ccacaacggt
      181 atagacccgt ccgttgcgtt tcagaatgcc gaagacaacc acttttcctg ccgcaccgcg
      241 accacgtctg cctttacgcc gtccgccgaa atcgctttcg tccggctcga cagggccctc
      301 aaaaacctca tcggcagcca aggccaaatg atggttgata accgtgcgga ttttacggta
      361 gaacagtgct gccgaattgg gatggatacc caaaatatcg gcggcagaac gggcggtaac
      421 ttcgagtaca aaaaaacgga gcagttcttt ctgtactttt ttctttaatt tgcagtgcgt
      481 tatcttcata tttcgagggt aacatatctg ctaatctagt acagccccta gtttaaagct
      541 ttgtttctta agtccgcaga gtatgccatg gttagacctt caaagttgag tattgtacta
      601 ttttgttttt aattaactat aaaacgcttg tgtgaatagg aagactgcag aatattgctg
      661 ccttttcctg tttgctttaa ataaacagaa aatgccgtct gaaaccgatt ttcagacggc
      721 atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca caggttgcct
      781 tccatccttc gtacaacctt tgctccatca aacatccact tcctaaatac tgatgtctgc
      841 ttaggttgaa tcggcattcg tacgattacg gcacaccgcc taatctgtac ctgcgtctgt
      901 tgcgcccaat accaaaggct acctgaaaat ctcccaaact tttcaggtag ccttatcacg
      961 ttttcgactg tcaccacacg cccaaagacc gcctgaaacc ctttttcaga cgttccactc
     1021 cgataccccc caacaaaacg ccgcccgaac acatgttcag acggcatttg atgcggctgc
     1081 agacttcccc ctattttatt ttttatccgc gggcagcact ggttt
//
LOCUS       DAXIHS010000167         1113 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXIHS010000167 DAXIHS010000000
VERSION     DAXIHS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.167"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            295..804
                     /locus_tag="ACFOF2_002045"
     CDS             295..804
                     /locus_tag="ACFOF2_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC7108284.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLVEGQKSAVTEYYLNHGEWPANNSSAGVATSANIKGKYVQSVEVKNGVVTATMLSSG
                     VNNEIKGKKLSLWAKRQNGSVKWFCGQPVTRDDAKATNDDVTADNGTDKKIDTKHLPS
                     TCRDASSAS"
BASE COUNT          346 a          304 c          221 g          242 t
ORIGIN      
        1 ccccacgcca ataaaaaagt gccgaaaatc ggcactaaaa ctgacaattt tcaacactgc
       61 tgacaaaaaa cggtaatccc ctgcttcctc aaaccacacc caaacgaaag aaaatgtaaa
      121 ataaaaacaa ccatataaag ataaacgcat aaattttcac ctcaaaacac aaacttggta
      181 ccaatcttgc tttataatac acaggtatcg caaaaaaata ccgatggtca aatacattgc
      241 ataatgccga tggcgtaagc ttgaagcatt tcctttccaa ttaggagtaa ttttatgaac
      301 acccttcaaa aaggttttac ccttatcgag ctgatgattg tgattgccat cgtcggcatt
      361 ttggcggcag tcgcccttcc tgcttatcaa gactacacag cccgcgcaca agtttccgaa
      421 gccatccttt tggtcgaagg tcaaaaatca gccgtcacag agtattacct gaatcacggc
      481 gaatggcccg ccaacaacag ttctgccggc gtggcaacct ccgccaacat caaaggcaaa
      541 tatgttcaaa gcgttgaagt caaaaacggc gtcgttaccg ccacaatgct ttcaagcggc
      601 gtaaacaacg aaatcaaagg caaaaaactc tccctgtggg ccaagcgtca aaacggttcg
      661 gtaaaatggt tctgcggaca gccggttacg cgcgacgacg ccaaagccac caacgacgac
      721 gttaccgccg acaacggcac cgacaagaaa atcgacacca agcacctgcc gtcaacctgc
      781 cgcgacgcat catctgccag ctaaggtaaa ttataccata aattttaaat aaatcaagcg
      841 gtaagtgatt ttccacggcc actcggatta atccgggtgg cttccttttt aaaggtttgc
      901 aaggcaagcg gggtcgtccg ttttggtgga aaataatata tcgactgcgc ttcaaggctc
      961 tgcgcgtacc tcattgccgc ccgtttaaac acgtggttta tctgacaggc gcgcaatccg
     1021 ccctctcatt taccgaacaa gcggactaga ttcccgccta ctcgggaatg atgaagctat
     1081 ccgcacggaa acctgcacca cgtcattccc aca
//
LOCUS       DAXIHS010000168         1101 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXIHS010000168 DAXIHS010000000
VERSION     DAXIHS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.168"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..509
                     /locus_tag="ACFOF2_002046"
     CDS             <1..509
                     /locus_tag="ACFOF2_002046"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC7108285.1"
                     /translation="VRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDNLKAQAERLGI
                     DGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYDTPVVTYDMAG
                     ISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVAALCSDDEIYR
                     TTMAAYTM"
     gene            529..>1101
                     /gene="pglH"
                     /locus_tag="ACFOF2_002047"
     CDS             529..>1101
                     /gene="pglH"
                     /locus_tag="ACFOF2_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HFC7108286.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNV"
BASE COUNT          251 a          339 c          287 g          224 t
ORIGIN      
        1 ccgtccgcgc cgtgcatctg atgctggaca ttttcaaaaa aatggtcgac cgcgatatgc
       61 ccgtacgcct gaacgtggcg ggcataggcg aagaaatgga caatttgaaa gcgcaggcgg
      121 aacgtctggg catagacggc aaagttacct tcctcggcgg cgtgcgcgat ttgaccgcct
      181 acttcaaaga cgtggatatt ttggtgaaca cgccccactg catcggcgac cacggcgcag
      241 gtgtgggcaa caacatctta gaagccggac tttacgacac acccgtcgtt acctacgata
      301 tggcgggcat ttccgaaatg gtcgtcaacg gggaaacggg ttactgcatc cccttcggcg
      361 acgaagaagc ctttatcgcc gccgtagaca cgctcgtcag acaaccgctg ttgcgcgaag
      421 caatgggcaa aaggctgcac gcacacgtcg ccgcactctg ttccgacgac gaaatctacc
      481 gtaccaccat ggcggcctat accatgtaat gccgtctgaa agccgccgat gaacatcacc
      541 atagtcgccc cttattgctc gctgccgtcc gaaccgtatt tcaaccgctt ttggtatctg
      601 gcagaacgat tgtcgcaatc gcacgacgtg ttgctgatta ccagccgttt ccgccactac
      661 gacaaatcat tcagacggca tgaagatgct gccgccactt caaacggcag gctgcgcgtc
      721 aagctgctgg acgaacctgg ctaccgaaaa aacgtctccc tcgcccgcgt cgccagccac
      781 cgcgtattcg tccgcaattt ggcacgctgg ctgcacagcc cgcaggcggc ggaacaggac
      841 atcgtctatt ctgcctatcc gctgatggcg accaacctgc tgttgggcaa acacaaagcg
      901 cgtttgggct acaaactgat tgtcgatgtg caggacgtat ggccggagtc tttctcctcg
      961 gtcgtaccat ttttgaaaaa agtaccgcac aagctgctgc cctttgcttc acgcgccaac
     1021 cgtgcctacc gctgcgccga cgcgctggtt gccgtatcgc agacttatct cgaccgcgcc
     1081 aaagaagcca atccgaacgt a
//
LOCUS       DAXIHS010000169         1038 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXIHS010000169 DAXIHS010000000
VERSION     DAXIHS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.169"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            68..1033
                     /locus_tag="ACFOF2_002048"
     CDS             68..1033
                     /locus_tag="ACFOF2_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HFC7108287.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQGQQYSAEKAQRQSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHH
                     QITLHHSTIYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPA
                     IVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICRLDSLKAEDTARAAVRALK
                     AHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPK
                     QTDFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT          332 a          315 c          216 g          175 t
ORIGIN      
        1 caactttccc taacagaaaa aggccagtat gcggtagcat acggcctttc ctgcaagaaa
       61 gattgccatg agctacacgc aactgaccca aggcgaacga taccacatcc aatacctgtc
      121 ccgccactgc accgtcaccg aaatcgccaa acagctgaac cgccacaaaa gcaccatcag
      181 ccgcgaaatc agacggcacc gcacccaagg gcagcaatac agcgccgaaa aagcccagcg
      241 gcaaagccgg actatcaaac agcgtaagcg acaaccctat aagctcgatt cgcagctgat
      301 tcagcacatc gacaccctta tccgccgcaa actcagtccc gaacaagtat gcgcctacct
      361 gtgcaaacac caccagatca cgctccacca cagcaccatt taccgctacc ttcgccaaga
      421 caaaagcaac ggcagcacgt tgtggcaaca tctcagaata tgcagcaaac cctaccgcaa
      481 acgctacggc agcacatgga ccagaggcaa agtccccaac cgtgtcggca tagaaaaccg
      541 acccgctatc gtcgaccaga aatcccgtat cggcgattgg gaagccgaca ccattgtcgg
      601 caaaggacag aaaagcgcat tattgacctt ggtcgaacgc gttacccgct acaccatcat
      661 ctgcagattg gatagcctca aagccgaaga cactgcccgg gcagctgtta gggcattaaa
      721 ggcacataaa gacagggtgc acaccattac catggataac ggcaaagagt tctaccaaca
      781 caccaaaata accaaagcat tgaaagcgga gacttatttt tgtcgccctt accattcttg
      841 ggagaaaggg ctgaatgaga acaccaacgg actcatccgg caatacttcc ccaaacaaac
      901 cgatttccgt aacatcagtg atcgggagat acgcagggtt caagatgagt tgaaccaccg
      961 accaagaaaa acacttggct acgaaacgcc aagtgtttta ttcttgaatc tgttccaacc
     1021 actaatacac tagtgttg
//
LOCUS       DAXIHS010000170         1023 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXIHS010000170 DAXIHS010000000
VERSION     DAXIHS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.170"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..242)
                     /locus_tag="ACFOF2_002049"
     CDS             complement(<1..242)
                     /locus_tag="ACFOF2_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFC7108288.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGDFGEFCKGL"
     gene            complement(526..828)
                     /locus_tag="ACFOF2_002050"
     CDS             complement(526..828)
                     /locus_tag="ACFOF2_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108289.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
BASE COUNT          319 a          226 c          199 g          279 t
ORIGIN      
        1 atgagacctt tgcaaaattc cccaaaatcc ccaaaattcc caccaagaca tttaggggat
       61 tttggggaat tttgcaaagg tctcggacag tattttgaac gcagtgcgcg taaatccgta
      121 tggaaaccat gaaatcccgc cacagccgcc agacatgcca agccgcattc tgatatttct
      181 gtttgcagga taacaggcag ctttttcttt aagcccaaag acaggttttg cagatggggc
      241 atagatttcc tttttgaaaa atagggatta ggaagttgga tgtattttag aaaggccgcc
      301 tgaaaaggtt tcagacgacc ttttgcgact agctgctatt ttatttaaag cttttctcta
      361 acaaacgagc taatattttt cctgtaataa aacagataaa aaacagcatc caatacgtca
      421 gattggaaaa atcggtcgta tagagaatca acatataaag aagcagcatg atgccgagtg
      481 cgatgaattg ataatgtttg gcaaacatca tgacctcctc aactattaag gcaaaccgcc
      541 tgaatattct cgttcaatcg tttcggcaat ttccctataa cgtcgatacc atgaccagtc
      601 gaaattttca atggcatggc tcgcaaacgt accaaattca ggcatcccta tgcggctacc
      661 tgctaaagct ccgattgtag ctccccaacc aggcggatta ctgaacgtat tgtttggcaa
      721 tcctaaagat ttatcaggat ttcccgtatc gttaagccct gcagattcgc aaatttcgca
      781 ataatatgaa ctttgttgcg cattacctga gcctccgacc caagtcattt catgaacaca
      841 tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt acaaatggta
      901 cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactataaaa ctctcatttt
      961 gaaactcctt gtatcgttaa tcaaacaatc aaaagggcag atgccctatc cttgctttta
     1021 caa
//
LOCUS       DAXIHS010000171          868 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXIHS010000171 DAXIHS010000000
VERSION     DAXIHS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.171"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..>868
                     /gene="pilC"
                     /locus_tag="ACFOF2_002051"
     CDS             <1..>868
                     /gene="pilC"
                     /locus_tag="ACFOF2_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC7108290.1"
                     /translation="ETRNYAIVMNKQNQPEVKREGSYSTLREKDRKRKFDFNANNGGG
                     GSVSFNNSDELVSRQNGTAVFGTATYLPPYGKVSGFDDKRLKERGNAVNWIHTTHPGL
                     IGYSYAGVVCRNSTGCPQLVYKTRFSFDNTGLAKNAGMLDSHPDPSRENSPIYKLKDY
                     PWLGVSFNLGSENTVKNSQSSNRLISSFSENNNNQTIVSTTESHPISLGDGQREHTAV
                     VYYLNAKLHLLNKKQIQNITDKTVQLGVLKPSIDVKTQRTGFGGILSFWASWDIKDTG
                     QIPVKLGLQQVKA"
BASE COUNT          260 a          210 c          203 g          195 t
ORIGIN      
        1 cggaaacccg taactacgct attgtcatga acaaacaaaa tcagccggag gtaaagcggg
       61 agggttcata ttcaacctta agggaaaaag acaggaaacg caaatttgac tttaatgcga
      121 acaacggggg tggaggctct gtctcattca acaatagcga tgagcttgtt tctcgacaaa
      181 acggtactgc cgtttttggc acagccacct acctgccgcc ctacggcaag gtttccggtt
      241 ttgatgacaa aaggctgaaa gagcgcggca atgccgttaa ttggattcat acgacccacc
      301 cagggttgat aggctacagc tacgccggtg tcgtatgcag aaacagcaca ggctgtcccc
      361 aacttgtcta taaaacccga ttttccttcg acaacaccgg tttggcaaaa aatgcgggca
      421 tgctggatag tcacccggac ccaagccgcg aaaattcgcc catttacaaa ttgaaagatt
      481 atccatggtt gggcgtatct ttcaatttgg gcagcgagaa taccgtcaaa aatagccaat
      541 catccaacag attgatatct tcttttagtg aaaacaataa taatcaaacc atcgtctcta
      601 cgacagaaag ccaccctatt tcccttggcg acgggcagcg cgaacatacc gccgtggtct
      661 attatctgaa cgccaagctg cacctgctga ataaaaaaca gattcaaaat atcaccgaca
      721 aaacagtgca gttgggtgtc ttgaagccga gcatcgatgt gaagacacaa aggacggggt
      781 ttggcggcat tctatctttt tgggctagtt gggacattaa agataccggg cagattccag
      841 tcaagctcgg cctgcagcaa gtcaaagc
//
LOCUS       DAXIHS010000172          857 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXIHS010000172 DAXIHS010000000
VERSION     DAXIHS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.172"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..>857
                     /locus_tag="ACFOF2_002052"
     CDS             <1..>857
                     /locus_tag="ACFOF2_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC7108291.1"
                     /translation="DWQPIEQYLNRQRTRYLRDHRGRPAYPLLSMFKAVLLGQWHSLS
                     DPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDNTLSELLKLINRQLTEK
                     GLKVEKASAAVVDATIIQTAGSKQRQAIEVDNKGQVSGQTTPSKDKDARWTKKNSLYK
                     LGYKQHTRTDAEGYIEKLYITPTNAHECKHLSPLLEGIAEGTTVYADKGYDSAENRQH
                     LEERQLLDGIMRKACRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFG
                     LICARCRLK"
BASE COUNT          243 a          259 c          194 g          161 t
ORIGIN      
        1 tgattggcag ccgatcgaac agtacctgaa tcgtcaaaga acccgttacc ttagagacca
       61 ccgcggccgt cccgcctatc ccctgttgtc catgttcaaa gccgtcctgc tcggacaatg
      121 gcacagcctc tccgatcccg aactcgaaca cagcctcatc acccgcatcg atttcaacct
      181 gttttgccgt tttgacgaac tgagcatccc cgattacagc accttatgcc gctaccgcaa
      241 ctggctggcg caagataata ccctgtccga attgctcaaa ctgattaacc gccaactgac
      301 cgaaaaaggc ctaaaagtag agaaagcatc cgccgccgtc gttgacgcca ccattattca
      361 gaccgccggc agcaaacagc gtcaggctat agaagtcgat aacaaaggac aagtcagcgg
      421 ccaaaccaca ccgagtaagg acaaagatgc ccgttggaca aagaaaaaca gcctctacaa
      481 actcggttac aaacaacata cccgtaccga tgcagaaggc tatatcgaga aactgtacat
      541 cactcccacc aatgcccatg agtgcaaaca cctgtcgcct ttgctggaag ggatagccga
      601 aggtacgacc gtctatgccg acaaaggcta tgacagtgcg gaaaaccggc aacatctgga
      661 agagcgtcag ttgttagacg gcattatgcg caaagcctgc cgcaaccgtc cgctgacgga
      721 aacgcaaacc aaacgcaacc ggtatttgtc gaagacccgt tatgtggttg aacaaagctt
      781 cggtacgctg caccgtaaat tccgctacgc tcgggcagcc tatttcggac tgatttgcgc
      841 ccgctgccgc ctaaaag
//
LOCUS       DAXIHS010000173          838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXIHS010000173 DAXIHS010000000
VERSION     DAXIHS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.173"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(182..748)
                     /locus_tag="ACFOF2_002053"
     CDS             complement(182..748)
                     /locus_tag="ACFOF2_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC7108292.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          238 a          274 c          172 g          154 t
ORIGIN      
        1 gcttcagacg gcattttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
       61 acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
      121 ctttgagcca aggcgaggca acgacgtact ggtttttgtt aatccactat attttccgac
      181 atcattgaat caaacccaaa tgcgacaaga gcgtccatgt gccgatggca atcaacacca
      241 aacctccggc aaattccgca cacctgccga acaatacgcc caaagccctt cccgccgtca
      301 gcccgaccgc caccatcacc gtcgtcgcca taccgatgat tgcggcggca aaggcgatgt
      361 ttacctccat aaacgccaag cccaccccga ctatcatgga atcaatactg gttccaaaag
      421 cagtcaaaac cgtcatccat aggctttccc gtttgctttc gcgcacatct tccgcctcgc
      481 cggacagccc ttcgcgcatc attttcagac ccagcccgcc cagcaggacg aaagccaccc
      541 aatggtccca ttcgctgata aacggcttgg cataaaaacc gcctacccag cctgccagcg
      601 gcgtgagcgc ttcaaccgtg ccgaacacca aagccgttgc cgcaattttg cgcggaggca
      661 ttctgaccgc cgcgcccttt gccaatgcga cggcaaacgc atccatcgac atccccagag
      721 caatcaagag caaagcataa aaacccatac cgcacccgtc ctcaaaaagg gcggattata
      781 gcaaaagcaa aaaaatgcaa aaatgccgca cgaaaacccg catcccgtca ttcccgca
//
LOCUS       DAXIHS010000174          784 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXIHS010000174 DAXIHS010000000
VERSION     DAXIHS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.174"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(34..354)
                     /locus_tag="ACFOF2_002054"
                     /pseudo
     CDS             complement(34..354)
                     /locus_tag="ACFOF2_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(433..>784)
                     /locus_tag="ACFOF2_002055"
     CDS             complement(433..>784)
                     /locus_tag="ACFOF2_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC7108293.1"
                     /translation="EMKSSGVNKEIQGKKLSLWAKRQNGSVKWFCGQPVTRAANNTAK
                     DAVTAANDNSNNGINTKHLPSTCRDAASAICTRTPRADFKHFQKISRYRVLPESRHMA
                     EKLRHSRKSGNLVR"
BASE COUNT          153 a          173 c          219 g          239 t
ORIGIN      
        1 aatttatcgg aaaaaacaga aaccgctctg ccgtcattcc cacgaaagtg ggaatccagc
       61 cttgtcggtg cggaaactta tcgggtaaaa aggtttcttt agtttggtgt cgattttgtc
      121 ggtgccggtg gcggcggcaa cgtcgtcttt ggcgttggcg gcgcgcgtaa ccggctgtcc
      181 gcagaaccat tttaccgaac cggcttgacg cttggcccac agggagagtt ttttgccttt
      241 gatttcgttg tttacgccgg ttgaaagcat tgtggcggta acgacgccgt ttttgacttc
      301 aacttcctta acatatttgc ctttgattgt tgaagaagat gccacgccgg cggcatcatt
      361 aaatcccgtc attcccactt tcgtgggaat gacgggatta aagtttcaaa atttattcta
      421 aataactgaa actcaacgaa ctagattccc gcttttgcgg gaatgacgaa gtttttcggc
      481 catgtgccgt gattcgggca atactcggta acggctgatt ttttgaaaat gtttgaaatc
      541 ggcgcgtggt gttcttgtgc aaatggcaga tgccgcatcg cggcaggttg acggcaggtg
      601 tttggtgttg attccgttgt tgctgttgtc gttggcggcg gtgacggcgt ctttggcggt
      661 gttgttggcg gcgcgcgtaa ccggctgtcc gcagaaccat tttaccgaac cgttttgacg
      721 cttggcccac agggagagtt ttttgccttg gatttctttg tttacgccgc ttgatttcat
      781 ttcg
//
LOCUS       DAXIHS010000175          770 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXIHS010000175 DAXIHS010000000
VERSION     DAXIHS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.175"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(408..>770)
                     /locus_tag="ACFOF2_002056"
     CDS             complement(408..>770)
                     /locus_tag="ACFOF2_002056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC7108294.1"
                     /translation="RLKNKKQPAQPVFLAANLNPNSRPVLSPCGRELERGQQAERLVL
                     GRLSCWERLPQLGKCRLPGPPLPRRRQWRACRRANLPDKTVFLRFYVPDSRFRGNGGP
                     RRRLRIAENRFTMISAQG"
BASE COUNT          153 a          223 c          216 g          178 t
ORIGIN      
        1 aaaacatcaa gatggcggca taaagcgcgg tatggcggaa aacctgcctt tttaaagttt
       61 tattcatcgt atttcctttt cggtcaaacc ccccgccctt cgggcatccg tccttcgggg
      121 cggcaggatc aggctttgtc cgggaggggt gcaagccctt ccgaatcagg gcggcgcctt
      181 atgtgccgcc ctgcgtgttg gaacatattt tatcccttaa atcttgcttc gcgggatacc
      241 gtcggcggca ggcgcgccgc aaacggcggg attcggcaaa ccgcccgatt ccgcgcccgc
      301 cgaattgctg gttggaaaac ccctttggcc cggctgccaa agggggatgt tcggaaaagt
      361 aacacgcccc tttggccgga tgcaatgtat agggttaccg cattattcta cccttgcgcg
      421 ctgatcatgg taaagcggtt ttcggcaatc cgcaaccgcc tccttggccc gccattgccg
      481 cgaaagcggg aatccggaac gtaaaatctc aaaaaaaccg ttttatccgg caagtttgcc
      541 cgccggcagg cccgccattg ccgcctgcgc ggcaatggcg ggccggggag gcggcatttt
      601 ccaagttgcg gcaatctttc ccaacaactc aaccgcccca atacaagcct ttcggcttgt
      661 tgccctctct ctagctctct cccacaggga gagaggacgg gtcggctgtt ggggttgagg
      721 tttgctgcaa ggaaaacagg ttgtgcaggc tgctttttat ttttcagacg
//
LOCUS       DAXIHS010000176          716 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXIHS010000176 DAXIHS010000000
VERSION     DAXIHS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.176"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(143..>716)
                     /gene="pilC"
                     /locus_tag="ACFOF2_002057"
     CDS             complement(143..>716)
                     /gene="pilC"
                     /locus_tag="ACFOF2_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC7108295.1"
                     /translation="RSDGSGNKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGNDKC
                     GAETAILGINTADGGKLTKKSARPIVPDANTKVAQYSGDKKTSSGKSIPIGCMQKGNE
                     IVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGV
                     RTLLMNDLDSLDITGPTCGMKRISWREIFY"
BASE COUNT          147 a          196 c          179 g          194 t
ORIGIN      
        1 attgaattgt ggaaattgat ggattcagtg agattggcga gatgaagcct accctatagc
       61 ccgcctttta cgaacccgcc cctcccgaaa aacgcaaaaa atgccgtccg aaaacctttc
      121 ggacggcatt ttcgcgtgca aatcagtaga agatttcacg ccagctgatt cgtttcatac
      181 cgcacgtcgg gccggtaatg tccaagctgt ccaaatcgtt catcagcagg gtgcgcaccc
      241 ctttttggga gaagcagcgg ttgtttttgc cggaacgctt gccggcgggg tctataccgc
      301 tgccgcccgc atcgccgtct gccgttgttg aaaatccgtc tgttttcttt tcatccagat
      361 aacgcacatt aaccggtttg tcgtaaacat atccgttcgg gcagacgatt tcattgcctt
      421 tttgcataca acctataggg atggatttgc cgctggaagt tttcttatcg ccggaatatt
      481 gcgcgacctt cgtattggcg tccggcacaa tcgggcgcgc gcttttcttg gtcagcttgc
      541 cgccgtcggc ggtattgatg cccaaaatgg cggtttccgc gccgcatttg tcattacccg
      601 tatatttatg gatggttaca aaggcggtac gcaataccac ggtcggtttg acggtaacgc
      661 gctgtccgtc cttcaatttc actacccagc ccttattgcc cgagccgtcg gatcgc
//
LOCUS       DAXIHS010000177          703 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXIHS010000177 DAXIHS010000000
VERSION     DAXIHS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.177"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            26..703
                     /locus_tag="ACFOF2_002058"
     CDS             26..703
                     /locus_tag="ACFOF2_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HFC7108296.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          217 a          136 c          156 g          194 t
ORIGIN      
        1 tcaaaaaacc tgataagctc ctaatatgaa actaaaaaat aagtatcaaa agttcagcaa
       61 aatttcagag caaaagttta gagaaataat ccgctgtttc gctcttgatt tgaccgcttc
      121 cgatactgct aaaatgaccg gcattagtgt acgcggtatc aatcctattt tcctgaaaat
      181 cagacacagg attgctgctc tgtgcgaaca aagctcacca ctgtccggtg tggtcgaatt
      241 agatgaatct tactttggtg cgcgacgtat caggggtaag cgcggtcgcg gagcgtcagg
      301 taaaaccatc gtatttggca tactgaaacg caatggcgtg gtctatacgg aaatcgttcc
      361 cgacgcttcg aaagcctcac taatcaaggt catacgtggt cacatatctg ctgacagtga
      421 aatcaatact gacggctgga aagcatatga cggtcttgtc gatatgggct atgagaagca
      481 ttaccgtgtc catcatggtt ccaatgagtt tgctcgagga aagcaacata tcaatggtat
      541 tgaatctttt tggagttata ctaaggggcg tttggctaaa tttcatggta tttccaaaga
      601 gatgttttat ctgcatctca aagaaacgga gtttaggttt aaccaccggc atgaagattt
      661 aggtaaaata ttaatgaaga tgcttcgaaa taacccaatt taa
//
LOCUS       DAXIHS010000178          698 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXIHS010000178 DAXIHS010000000
VERSION     DAXIHS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.178"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(<1..698)
                     /locus_tag="ACFOF2_002059"
     CDS             complement(join(<1..479,479..698))
                     /locus_tag="ACFOF2_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC7108297.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARN"
BASE COUNT          161 a          161 c          136 g          240 t
ORIGIN      
        1 attacgagct aatttctcat agcgagtagc aatactacga aactgcttta atcgtgcaaa
       61 tgcattctcg actaagtggc gtaatttata aaggtagcta tcaaaatcag ggttaggttt
      121 cttggcattt ttacgctttg gtataatagc tttcataccg tgttctatcg ctttatctct
      181 gatttcttgc gagtcatacc ctttgtcagc gataaaatac tgggcttctt gtatgacttc
      241 tatcaagtcg tttgcaactt gactgtcgtg cacgttaccc ccagtgactt taaaatcgag
      301 cggatttcca tgcgagtcca cacataggtg tatttttgtc gtgtttccgc cacggctttg
      361 tccaattgct ctatcgaaac cacgccgagc tccacttgca tgttgatgac accgtacata
      421 acttccgtcg atgaataccc attctttgtc aatttctttt cgtagatcaa aaaaaaattc
      481 tgccacaagc cttttttaga ccatcggtta aagcggttat aagcggtttt ccatgaccct
      541 agctcaatag gtatgtctcg ccatggtgca cctgttctta gcttccatag tatggcttcc
      601 atcacggtac ggtcgttctt ccattgatga cagccgtgcg ctttcatggt tgtttgtaat
      661 tgttcccata tgttgtcagt tattgcggtt ctcgccat
//
LOCUS       DAXIHS010000179          690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXIHS010000179 DAXIHS010000000
VERSION     DAXIHS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.179"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(152..>690)
                     /locus_tag="ACFOF2_002060"
     CDS             complement(152..>690)
                     /locus_tag="ACFOF2_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC7108298.1"
                     /translation="GGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGNDYL
                     EGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQ
                     DNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGN
                     VDANTQSVQQPLLVTPSA"
BASE COUNT          201 a          162 c          128 g          199 t
ORIGIN      
        1 gtactggttt ttgtgaatcc actatattgc tagaagtgca ccgattaagg ttaattaaaa
       61 tacgaccctt atcccaatat taagaaaaac agcaatcaaa ataaacttga ttgctgtttt
      121 tcagtttaag ccatgaatgt gattaggctc cttatgcaga tggcgttacc aataacggct
      181 gctgtacgga ttgtgtattg gcatccacat ttcctcctgt cgcagcagta ttagaaccga
      241 acacagacat agactgtacc aaattattgc cagcattaat atacttggca aaatctggat
      301 tactgatagc tgcatcatca aagacaaatg tatctacacg atggttttca ccatagaaga
      361 atccggaaat acgtacgttg tcttgttcag aagcgctaag caccaaatca cttccggaac
      421 ggataaaatg aacatctgct gctttaaatc ctttaaagtg catagtgtca gagtttttat
      481 ccacatggta attatagacc gtatcctgac cgaagccttt gccgaagaca taagtatccg
      541 aaccgctgcc gccctccaag taatcattgc ctgcaccgcc gattagagtg tcgttaccgt
      601 cttcgccgtt caaatgatca ttgccttcgc caccattcag tacatcgtta ccattatagc
      661 cgtacagggc gtcgttgcct tctcctccat
//
LOCUS       DAXIHS010000180          541 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXIHS010000180 DAXIHS010000000
VERSION     DAXIHS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.180"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
BASE COUNT          118 a          124 c          151 g          148 t
ORIGIN      
        1 tctcaacagc ttaaaatcct gcgggattgg tgtggaattt agggctaatc tagtacagcc
       61 ccttagattt aattcactat acctgcgtaa tggtatcggg tactcatcat caacctgcct
      121 tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc gatgggttgc cctgccgctc
      181 cctgtgctac gcaacggttg tgtgccttgg gtgggcgcgg gtggcggccg ggcggtttgt
      241 tgctatgata cgggagttcc gatatacaag ggtgggcttc agcccaccaa tcaacgaccg
      301 aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat tacgcaactg ttatcgtggg
      361 gatgacggga ttttaggttt ttgtttttct gtttttgcgg gaaggatggg ttttaggttg
      421 cggtcattta tcggaaaagc agaaatcgct ccgccgtcat tcccgcgaaa gcgggaatct
      481 gaacacgtcc gtagggaaac ctatatcccg tcattcccac gaaagtggga atctagaacc
      541 t
//
LOCUS       DAXIHS010000181          512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXIHS010000181 DAXIHS010000000
VERSION     DAXIHS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.181"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
BASE COUNT          126 a          135 c          119 g          132 t
ORIGIN      
        1 ttcgcgggaa tgacggggcg gtcggatgcc gcctgaaact ccgtcattct tgcaactttt
       61 cgtcattccc gtgaaaacgg gaatctagaa cttcaaactt tcagataatc tttgaatatt
      121 gccgttgccc gacggtctag attcccgctt gcgcgggaat gacggtagag cggtttcagt
      181 tgctcccgat aaatgccgca atctcaaatc ccgtcattcc ctcaaaaaca gaaaaacaaa
      241 atcagaaacc taaaatcccg tcattcccgc agctcttcgt cattcccgtg aaagcgggaa
      301 tctagacctt ttaaattttc acacggcttt tgaatattgc cgttacccga aggtctggat
      361 tcccgcctgc acgggaatga cggcagagcg gtttcagttg tttctaaata aatttttgca
      421 gcgttggatt tttagattcc cgctttcgcg ggaatgacgg ggcggtcgga tgccgcctga
      481 agcccgacaa agcccccaat gccgtctgaa ac
//
LOCUS       DAXIHS010000182          491 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXIHS010000182 DAXIHS010000000
VERSION     DAXIHS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.182"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            270..>491
                     /locus_tag="ACFOF2_002061"
     CDS             270..>491
                     /locus_tag="ACFOF2_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFC7108299.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLN"
BASE COUNT          113 a          140 c          108 g          130 t
ORIGIN      
        1 gtatcgctgt aaatcttaga gatggcggaa tatagcggat taacaaaaac cagtacggcg
       61 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
      121 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
      181 ctatagatta tcatttatcc ttttctaaag ccgttccggt ttgtccgacc ggcggctttg
      241 ccccaatatc cccattttgg agacacccta tgttgcgttt gaccgcattg accctctgca
      301 ccgtcctcgc cttgggcgcg tgttcgccga aagattccga ctctgcccca caagccaaag
      361 aacaggcagt ttccgccgca caatccgaaa gcgcgtccgt taccgtcaaa accgcgcgcg
      421 gcgatgttca aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg
      481 acactttaaa c
//
LOCUS       DAXIHS010000183          488 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXIHS010000183 DAXIHS010000000
VERSION     DAXIHS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.183"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            complement(161..472)
                     /gene="rpsJ"
                     /locus_tag="ACFOF2_002062"
     CDS             complement(161..472)
                     /gene="rpsJ"
                     /locus_tag="ACFOF2_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC7108300.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT          121 a           97 c          123 g          147 t
ORIGIN      
        1 caatcatggc ttgggcggtt tgttggaaga aggtgctcat gagaaatccc ctaaatgtct
       61 tggtgggaat ttaggggatt ttggggaatt ttgcaaaggt ctcggcataa cataaaaaat
      121 ccccaagcat tgattgcttg gggattttca atagtccgaa ttattggact ttgatttcta
      181 catcaacacc ggccggcaaa tccagcttca tcagcgcatc ggtagtttta tcggtccaat
      241 ccacgatgtc catcaggcgc aagtgggtgc ggatttccaa ttgttcacgg gaagttttgt
      301 tcatgtgcgg agaacgcaga atgttgaaac gttcgatttt ggtcggcaac ggaatcggac
      361 cttttacaac ggcaccggta cgttttgcag tttcaacgat ttcttgtgca gaacggtcaa
      421 tcagggcgta atcataagct ttcaggcgga tacggatttt ttggtttgcc atttatcaat
      481 atccttca
//
LOCUS       DAXIHS010000184          453 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXIHS010000184 DAXIHS010000000
VERSION     DAXIHS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.184"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
BASE COUNT          119 a          139 c           99 g           96 t
ORIGIN      
        1 aaaccccgag attctagatt cccacttccg tgggaatgac ggttcagttg cgttccgaca
       61 acaccgtaat ctcgaaaccc gtccgacaac accgtaatct tgaaattcgt cattcccgca
      121 caggcgggaa tccagaccca cgacgcggcg ggaatctatc ggaaatgact gaaaccccga
      181 gattctagat tcccacttcc gtgggaatga cgattcagtt gcgttccgac aacaccgcaa
      241 tctcgaaacc tgtcattccc gcacaggcgg gaatccaaac ctgtccgcac agaaacttat
      301 cggataaaaa cagttgccca aaccccgcgt tctagattcc cactttcgtg ggaatgacgt
      361 ggtgcaggtt tccgtatgga tggattcgtc attcccgaca acaccccaat cttgaaaccc
      421 gtcattcccg cacaggcggg aatccagacc tgt
//
LOCUS       DAXIHS010000185          388 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXIHS010000185 DAXIHS010000000
VERSION     DAXIHS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.185"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            1..388
                     /locus_tag="ACFOF2_002063"
                     /pseudo
     CDS             1..388
                     /locus_tag="ACFOF2_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          117 a           94 c           85 g           92 t
ORIGIN      
        1 ccaccatttg ctatgcactc aaacagatgg ggataacgcg caaaaaaaga ccaccactta
       61 caaagaacaa gacccggcca aagtaacgca ttatttgaca cagccggccg aattttccga
      121 ctaccaacgt gtttatttgg atgaaacagg atttgaccgc tacctgttcc gtccctatgc
      181 ccgcagcctg aaagggcaaa tagtgaaagc gcagataagt ggaaaaagat accaacgctt
      241 atctttggtg tccgcacaag tcggcaaccg gctgattgct ccgatggttt atcaaaatac
      301 gatgaccgga gtcttttttg aagcgtggtt tcagcaatgc ctactgcccg cattgactca
      361 aaaatcggtg attattttag ataatgcg
//
LOCUS       DAXIHS010000186          384 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXIHS010000186 DAXIHS010000000
VERSION     DAXIHS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.186"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            119..>384
                     /locus_tag="ACFOF2_002064"
     CDS             119..>384
                     /locus_tag="ACFOF2_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC7108301.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQD"
BASE COUNT          123 a           51 c          104 g          106 t
ORIGIN      
        1 gtgggaatga cgaagagttg cgggaatgat ggaaagctat gggaataacg aagggttaaa
       61 gtaatcacgg gatggtgttc gcgggaatat aaattgaaat aattcaaaag ggtattatat
      121 gcagcctgcg gtttatattt tagcaagcca acgtaatggc acgttataca ttggcgttac
      181 atctgatttg gtgcaacgta tttaccaaca tagggagcat ttgattgagg gatttacatc
      241 acggtacaac gttactatgc tggtttggta tgaactgcat cctacgatgg agagtgcaat
      301 tactcgggaa aaacagttga agaaatggaa cagggcttgg aaattgcaac tgattgaaga
      361 aaataatgtt tcttggcagg attt
//
LOCUS       DAXIHS010000187          381 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXIHS010000187 DAXIHS010000000
VERSION     DAXIHS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.187"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
BASE COUNT          108 a          102 c           69 g          102 t
ORIGIN      
        1 cgggaatgac ggcggagggt ttttagtttt cccgaaaatg cacatcatcc aaaatcccgt
       61 tattcccaca aaacagaaaa tcaaaaacag taacctgaaa tcccgtcctt ccctcccgtg
      121 cgggaatccg acttatctgg tttcggttct ttttctcgtt tcaggtgatt tctaaaccgt
      181 cattcccgtg caggcgggaa tccagacctt taaaccccgg acatccccga taaattcttg
      241 cggcattgaa attctagatt cccgctttca cgggaatgac ggcggagggt ttttagtttt
      301 cccgataaat gcacatcatc caaaatcccg ttattcccac aaaacagaaa atcaaaaaca
      361 acaatctgaa attccgtcct t
//
LOCUS       DAXIHS010000188          374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NIID618
            SAMD00320054-rid22927783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXIHS010000188 DAXIHS010000000
VERSION     DAXIHS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00320054
            Sequence Read Archive: DRR291383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002330275.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002330275.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 11:34:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,011
            Genes (coding)                    :: 1,897
            CDSs (with protein)               :: 1,897
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 50 of 114
            Pseudo Genes (incomplete)         :: 75 of 114
            Pseudo Genes (internal stop)      :: 11 of 114
            Pseudo Genes (multiple problems)  :: 20 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00320054-rid22927783.denovo.188"
                     /strain="NIID618"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="Japan: Kanagawa"
                     /collection_date="2016-02-11"
     gene            <1..>374
                     /locus_tag="ACFOF2_002065"
     CDS             <1..>374
                     /locus_tag="ACFOF2_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC7108302.1"
                     /translation="YATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVTVQS
                     YFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTDGSDRLYAYQSGNTLNGGLGDD
                     YLYGADGDDLLNGDAGNDSIYS"
BASE COUNT          103 a           90 c           94 g           87 t
ORIGIN      
        1 actacgctac cggacgcaaa gacatcatcc gctttaccga cggtattaca gccgatatgc
       61 tgacttttac ccgagagggc aaccatcttc ttatcaaggc aaaagacggc agtggacaag
      121 tgactgttca gtcctatttc cagaacgatg gctcaggtgc ttaccgtatc gatgagattc
      181 atttcgataa cggcaaagta ctggatgttg ccactgtcaa agaactggta cagcaatcca
      241 ccgacggttc ggacagattg tatgcctacc aatccggaaa taccttaaat ggcggattgg
      301 gcgatgacta tctgtacggt gccgacgggg atgacctgct gaatggtgat gcaggcaacg
      361 acagtatcta cagt
//
